LOCUS       DAYYHX010000001        50615 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000001 DAYYHX010000000
VERSION     DAYYHX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.001"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            558..3197
                     /locus_tag="ACHA6Y_000001"
     CDS             558..3197
                     /locus_tag="ACHA6Y_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8986131.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3365..4063)
                     /locus_tag="ACHA6Y_000002"
     CDS             complement(3365..4063)
                     /locus_tag="ACHA6Y_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8986132.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            4281..5426
                     /gene="tgt"
                     /locus_tag="ACHA6Y_000003"
     CDS             4281..5426
                     /gene="tgt"
                     /locus_tag="ACHA6Y_000003"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM8986133.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            5523..5903
                     /gene="yajC"
                     /locus_tag="ACHA6Y_000004"
     CDS             5523..5903
                     /gene="yajC"
                     /locus_tag="ACHA6Y_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM8986134.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            6208..8805
                     /gene="adhE"
                     /locus_tag="ACHA6Y_000005"
                     /gene_synonym="adhC"
     CDS             6208..8805
                     /gene="adhE"
                     /locus_tag="ACHA6Y_000005"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM8986135.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            9034..10140
                     /locus_tag="ACHA6Y_000006"
     CDS             9034..10140
                     /locus_tag="ACHA6Y_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8986136.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            10157..11185
                     /locus_tag="ACHA6Y_000007"
     CDS             10157..11185
                     /locus_tag="ACHA6Y_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM8986137.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            11191..11670
                     /locus_tag="ACHA6Y_000008"
     CDS             11191..11670
                     /locus_tag="ACHA6Y_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM8986138.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            11686..12981
                     /locus_tag="ACHA6Y_000009"
     CDS             11686..12981
                     /locus_tag="ACHA6Y_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM8986139.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            12992..13816
                     /locus_tag="ACHA6Y_000010"
     CDS             12992..13816
                     /locus_tag="ACHA6Y_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8986140.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            13981..14880
                     /locus_tag="ACHA6Y_000011"
     CDS             13981..14880
                     /locus_tag="ACHA6Y_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8986141.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(15006..15875)
                     /locus_tag="ACHA6Y_000012"
     CDS             complement(15006..15875)
                     /locus_tag="ACHA6Y_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8986142.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            16028..17488
                     /gene="rhaB"
                     /locus_tag="ACHA6Y_000013"
     CDS             16028..17488
                     /gene="rhaB"
                     /locus_tag="ACHA6Y_000013"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM8986143.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            17485..18741
                     /gene="rhaA"
                     /locus_tag="ACHA6Y_000014"
     CDS             17485..18741
                     /gene="rhaA"
                     /locus_tag="ACHA6Y_000014"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM8986144.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            18755..19600
                     /gene="rhaD"
                     /locus_tag="ACHA6Y_000015"
     CDS             18755..19600
                     /gene="rhaD"
                     /locus_tag="ACHA6Y_000015"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM8986145.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            19602..19913
                     /gene="rhaM"
                     /locus_tag="ACHA6Y_000016"
     CDS             19602..19913
                     /gene="rhaM"
                     /locus_tag="ACHA6Y_000016"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM8986146.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            19995..21146
                     /gene="fucO"
                     /locus_tag="ACHA6Y_000017"
     CDS             19995..21146
                     /gene="fucO"
                     /locus_tag="ACHA6Y_000017"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM8986147.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            21273..22031
                     /locus_tag="ACHA6Y_000018"
     CDS             21273..22031
                     /locus_tag="ACHA6Y_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM8986148.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            22131..22775
                     /locus_tag="ACHA6Y_000019"
     CDS             22131..22775
                     /locus_tag="ACHA6Y_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8986149.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            22782..23789
                     /locus_tag="ACHA6Y_000020"
     CDS             22782..23789
                     /locus_tag="ACHA6Y_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8986150.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            23805..24647
                     /gene="kduI"
                     /locus_tag="ACHA6Y_000021"
     CDS             23805..24647
                     /gene="kduI"
                     /locus_tag="ACHA6Y_000021"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM8986151.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            24667..25470
                     /locus_tag="ACHA6Y_000022"
     CDS             24667..25470
                     /locus_tag="ACHA6Y_000022"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM8986152.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25719..26783)
                     /gene="fni"
                     /locus_tag="ACHA6Y_000023"
     CDS             complement(25719..26783)
                     /gene="fni"
                     /locus_tag="ACHA6Y_000023"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM8986153.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26780..27865)
                     /locus_tag="ACHA6Y_000024"
     CDS             complement(26780..27865)
                     /locus_tag="ACHA6Y_000024"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM8986154.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(27878..28855)
                     /gene="mvaD"
                     /locus_tag="ACHA6Y_000025"
     CDS             complement(27878..28855)
                     /gene="mvaD"
                     /locus_tag="ACHA6Y_000025"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM8986155.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(28848..29792)
                     /gene="mvk"
                     /locus_tag="ACHA6Y_000026"
     CDS             complement(28848..29792)
                     /gene="mvk"
                     /locus_tag="ACHA6Y_000026"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM8986156.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            30108..30767
                     /locus_tag="ACHA6Y_000027"
     CDS             30108..30767
                     /locus_tag="ACHA6Y_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8986157.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            30852..31757
                     /locus_tag="ACHA6Y_000028"
     CDS             30852..31757
                     /locus_tag="ACHA6Y_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8986158.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(31759..32391)
                     /locus_tag="ACHA6Y_000029"
     CDS             complement(31759..32391)
                     /locus_tag="ACHA6Y_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8986159.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    32542..32667
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            32711..33235
                     /gene="infC"
                     /locus_tag="ACHA6Y_000030"
     CDS             32711..33235
                     /gene="infC"
                     /locus_tag="ACHA6Y_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM8986160.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            33307..33507
                     /gene="rpmI"
                     /locus_tag="ACHA6Y_000031"
     CDS             33307..33507
                     /gene="rpmI"
                     /locus_tag="ACHA6Y_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM8986161.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            33560..33919
                     /gene="rplT"
                     /locus_tag="ACHA6Y_000032"
     CDS             33560..33919
                     /gene="rplT"
                     /locus_tag="ACHA6Y_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM8986162.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            34171..35508
                     /locus_tag="ACHA6Y_000033"
     CDS             34171..35508
                     /locus_tag="ACHA6Y_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM8986163.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            35556..37685
                     /locus_tag="ACHA6Y_000034"
     CDS             35556..37685
                     /locus_tag="ACHA6Y_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM8986164.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            37660..38349
                     /locus_tag="ACHA6Y_000035"
     CDS             37660..38349
                     /locus_tag="ACHA6Y_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986165.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(38757..39370)
                     /locus_tag="ACHA6Y_000036"
                     /pseudo
     CDS             complement(38757..39370)
                     /locus_tag="ACHA6Y_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(39506..39700)
                     /locus_tag="ACHA6Y_000037"
     CDS             complement(39506..39700)
                     /locus_tag="ACHA6Y_000037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986166.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(39750..40190)
                     /locus_tag="ACHA6Y_000038"
     CDS             complement(39750..40190)
                     /locus_tag="ACHA6Y_000038"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM8986167.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(40194..41039)
                     /locus_tag="ACHA6Y_000039"
     CDS             complement(40194..41039)
                     /locus_tag="ACHA6Y_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM8986168.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            41188..42606
                     /gene="pepV"
                     /locus_tag="ACHA6Y_000040"
     CDS             41188..42606
                     /gene="pepV"
                     /locus_tag="ACHA6Y_000040"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM8986169.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(42663..43610)
                     /locus_tag="ACHA6Y_000041"
     CDS             complement(42663..43610)
                     /locus_tag="ACHA6Y_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8986170.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(43833..44183)
                     /locus_tag="ACHA6Y_000042"
     CDS             complement(43833..44183)
                     /locus_tag="ACHA6Y_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8986171.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            44383..45462
                     /gene="pepA"
                     /locus_tag="ACHA6Y_000043"
     CDS             44383..45462
                     /gene="pepA"
                     /locus_tag="ACHA6Y_000043"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM8986172.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            45606..45926
                     /locus_tag="ACHA6Y_000044"
     CDS             45606..45926
                     /locus_tag="ACHA6Y_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8986173.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            45948..46412
                     /locus_tag="ACHA6Y_000045"
     CDS             45948..46412
                     /locus_tag="ACHA6Y_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8986174.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            46617..47222
                     /gene="ytpR"
                     /locus_tag="ACHA6Y_000046"
     CDS             46617..47222
                     /gene="ytpR"
                     /locus_tag="ACHA6Y_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM8986175.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(47261..48550)
                     /locus_tag="ACHA6Y_000047"
     CDS             complement(47261..48550)
                     /locus_tag="ACHA6Y_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM8986176.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            48978..49457
                     /gene="rlmH"
                     /locus_tag="ACHA6Y_000048"
     CDS             48978..49457
                     /gene="rlmH"
                     /locus_tag="ACHA6Y_000048"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM8986177.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            49542..50051
                     /locus_tag="ACHA6Y_000049"
     CDS             49542..50051
                     /locus_tag="ACHA6Y_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFM8986178.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            50151..>50615
                     /locus_tag="ACHA6Y_000050"
     CDS             50151..>50615
                     /locus_tag="ACHA6Y_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986179.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENI"
BASE COUNT        16085 a         9183 c        10867 g        14480 t
ORIGIN      
        1 aaaaagccga gctctcgata agctcggctt tcttgtacct cggaaggatt caaataaaca
       61 gaaatgattg taactttcca tcatacatgc aaagcacggt gttgaatttc ttcatgttaa
      121 acaaaaattt tgttttcgtt acatcttcaa catttgaaac atctcttgtt cctattcaat
      181 tttcagacgt ttacgttatt caattgttac gtatcgcatt acttcttgtt tcggatactt
      241 cgtgatccaa acatccatac gcattcacta tttataggtt tgtactctac acgcatgtct
      301 gggtgaaacc tgtacttcta acaactttgt ttgcttacac tgttctatcg agaaattctt
      361 ctgtctattt gatggtatcc tccttcctta catctatata atagcatcct ttggtagcgt
      421 ttgcaaatat aagcacttta aattttacgc tttcttaaca aataatcgat aagcccttta
      481 tttgaagtaa ataaaaaaag gatgttatag taaaataagt accttttttt gtacgaagat
      541 tttaaaggag gaataggatg gaagaataca agcagacaat agaagaagta aagaaggtaa
      601 cagaagcaga tccagatact gggttgtctt cacaagaagt ggatgaacgg cgaaaaaagc
      661 agggattgaa taaattcgat gaggcgccga aagaatcgat gatcaaaaaa ttctttcgaa
      721 gcttgtccga ttttacaacg atcatccttc ttgtagcagc ggtgatttcc ttttataccg
      781 catttgctac agaacatggg gatttatttg aaggactgct gatcatcgct atcgttgtga
      841 tcaactcggt attggcgatt gtccaagaag gaaacgcaga aaaagcactg gaatccttgc
      901 aagatatgaa taaacaaacg gcaactgtca tacgagacgg caaagtgacc actgttgagt
      961 cagagcaact ggtagtgggt gatattcttg ttttagagtc cggtgatgct atttctgccg
     1021 atgcccgttt gatcgaagca tcacaattac gtgtagaaga atcggcgttg actggagaaa
     1081 gtgaagcagt tgaaaaagat gcagcattta ttgctgaaga agatgagtca ttaggtgacc
     1141 aacttaatat ggtatttaaa ggatgtacgg ttgctgcagg tcgtgggaaa gcgatcgtca
     1201 ctgccattgg catggctaca gaaatgggta agatcgcgga tcttttgaac gaaaatacta
     1261 tgcaaaaaac acctttacag gtccgattga atcagctagg caaacgaatc agtatgatcg
     1321 ctttagcggc tgcggcttta gtatttgtga ttggtgaact gcaaggagaa ccactccttg
     1381 agatgttcat gacttcgatt tccttggctg ttgcagctgt accggaaacc ttgactgtta
     1441 ttgttacgtt gacacttgca tacggtgtgc agaagatggc gaggaaacat gcgatcatcc
     1501 gacagttgcc agcagttgaa acgctaggaa ccgctaatgt tatctgctca gacaaaacag
     1561 gaacgctgac acaaaatgaa atgcgcgtcc gccgggtatg gagcaaagaa gatgaagtaa
     1621 cgaatatcga agattcaatg acgaacagcg cgatggaaat attgaaaatg gctgcactat
     1681 gtacagatgt gacggtcgag caagaagatg acgaccttgt tatcaaaggt aatccaacag
     1741 aagtagccat cgtaagggct gttgaagaga attatcatac aaaggcagaa ttagaagaaa
     1801 aatatccacg agtaaacgag cttccttttg attctgaacg aaaaatgatg acaacagttc
     1861 accaaatggg cgaaaaatac atctcaatca ccaaaggagc attcgatgtt cttgctccgc
     1921 gctttagttc aggcgatgta gaacaagcgg cgatcgttaa tgatcgcttt ggtaaacggg
     1981 cactgagagt gattgctgtt ggttatgcta cttatgacga agaaccgcaa gatatttctt
     2041 ctgaagcact ggaaaaagac ttacgtttga ttggtctgat cggaatgatc gatccaccac
     2101 gtccagaaag taaaggagca atcaagcgag cgaaaaaggc tggcatcaaa acagtcatga
     2161 tcactggaga tcatgtggtg actgccagtg ccatagcgaa agaattaggt atcttgaatg
     2221 atccgtcaga agcactttca ggatcagaac tgcatcaatt atcagacgaa gaattagatg
     2281 cgcgtgtcaa agctctctct gtctatgctc gggtaacgcc agaagacaag atccggatcg
     2341 tcaaatcttg gcaacgtacg ggtgcagtcg tagcgatgac tggggatggt gtgaatgatg
     2401 caccagcgtt gaaagcaagt aacgttgggt gtgccatggg gatcacagga acagatgtgg
     2461 cgcaaagtgc cgcagatatg attttgacgg atgacaactt tgcgacgatc gtagacgctg
     2521 tcagtcaagg acgttcggtt tatcagaata ttcgtaaagc tatcaacttt ttgctaagct
     2581 gtaatatttc ggagatcttt attgttttga ttgcgatgct gttaggatgg ggagcaccat
     2641 ttacagctgt gcaactactg tttgtcaatg tagttgctga tggacttcca ggatttgctt
     2701 taggaagaga gccagcggaa cacggcatca tggatcagcc acccattccg aaaaatgaag
     2761 ggatctttgc tcgaggttta ttgcaaaaaa ttgccataaa tgcaggggtc ttcaccatcg
     2821 taaccttgtt tggttattat ttaggtagtt atgtcgatac tatcagccca tgggtagacg
     2881 caagtcagca tgtcggtcaa actgttgcat tcttgatctt agcttattca tcgattctgc
     2941 atgtgttcaa tgtacgcagc agccaatcga tcttcaaagt caatttagcg accaataaag
     3001 ctttggtaga aatggcgtta ctggcattag ctattacaac ggctgttgct ttgctgccat
     3061 ttacgcaaga actattcggc ctagtacaca tcagtttgaa tcactggttc ttggtaggga
     3121 ttttatctat cgtaccaatc gcggtgaatg aactgatcaa gttccatcga ttaccggaag
     3181 cagaagaaga ggaatgatta actgtttcta agttgatttt ctttttactg gaactataca
     3241 aaaaggaggt gtgacatgaa tgttgtcaca cctccttttt gctaatgggc tgaatgtttc
     3301 ttttactgcc tctcgataaa cggtgttctc ctgccacccc ctcggtcaca agtcacactc
     3361 tcttttaaat atcttttggc aattgttcat aaaatgctgc ttttgttgca gaaatgtatg
     3421 ggctgagcca tagataacca ataccagcag ttgctaatgc taagatatgc cagccgataa
     3481 atgaaagatc taaccagaaa agttgtcctt tataaccatc catcaatttt cgacttgcag
     3541 tgatcgtatc cagtacttta ggtgtttgtc ctgtttcttg gacgatatcg tagtaaataa
     3601 aatagctttg agaataagaa tagcttttaa tgattccagg aataactaat agcaaacccc
     3661 ataatgttgt aaagatcgag atcagcacag ctaagctgat cactccgccg ataaacaaac
     3721 ctttgaatcc acgaaatgca tctttgaatg gttcgatctg atctttttgt ccacgtaaaa
     3781 tatcaagata agtccaagaa ataccgctca tgaacaaagc gctaatgatg ctggaaataa
     3841 ttccgccacc gcctcctgaa ctaccgcctc ctgaagttgt cgcttctgag acgctcgctg
     3901 atccatcata tatggtggtg gcaaacaaga taataggaat cataataaga agaatagtag
     3961 caataccaat caatgtaggg attagattta gtaaaacagc ttgtccccaa cgtcctttta
     4021 gactctcttt tgcttggcgt tttaattctg cagaagattg catgattttt ccctcctttt
     4081 ctttaataac accaatatac catgtatcaa aaaatatttc tgtcataatg tttttctttt
     4141 ttttgttatt cataaaaaaa ggataaaaaa aagaatatta aataaagtag aaggaagccg
     4201 agggaacttg caaaaagcac ttgaaacgat tatgatggtg ggagttgtca agatatttta
     4261 tatagaaagt agggaaatga atgacgcaac ctgccattcg ctatcgttta atcaagaaag
     4321 aaaaacatac tggcgcgcgt ttaggggaat tgatcacgcc acacggaact ttccccacac
     4381 caatgttcat gccagtagga acattagcta cagtaaaaac aatgtcccca gaagagttga
     4441 aagaaatggg tgctggcgtt attttaagta atacatatca tttatggcta cgccctggtg
     4501 aagacttagt agaagaagct ggcggactgc ataaatttat gaactgggat caaccgattt
     4561 taacagattc aggcggcttt caagtttttt cattaagtga tatgcggaat atcgaagaag
     4621 aaggcgtgca tttcaaaaat cacttgaacg gttcgcgtat gtttttatca ccagaaaaag
     4681 caatcaacat ccaaaataaa ttgggctctg atatcatgat gagttttgac gaatgtccac
     4741 cattcgatga aagttacgac tacgtgaaaa aatcagtaga acgtacgtct cgatgggcag
     4801 aacgaggatt gaaagctcat gctaatccag accgtcaagg attatttgga atcatccaag
     4861 gagcagggtt tgaagatctt cgtcgtcaaa gcgcgaaaga tctagtcggg atggatttcc
     4921 ctggttactc aatcggcggg ttatctgtcg gggaacctaa atcagaaatg aatcgtgtat
     4981 tagaattcac tacaccgttg atccccgaaa acaaaccacg ttacttgatg ggtgtcggtg
     5041 ctgctgattc attgatcgat ggtgttatcc gtggagtaga catgtttgac tgtgtcttgc
     5101 ctactcgaat cgctcgaaat ggcacatgta tgacttctca aggccgtcta gtagtgaaaa
     5161 atgcgaaata tgcccatgat ttccgtccaa ttgatgagaa atgtgattgt tatacatgta
     5221 aaaactatac acgtgcctat atccgccatt tgatcaaatg tgatgaaaca tttgggattc
     5281 gtttgacttc ttaccacaat ttatacttct tgttgaatct aatgaaacaa gtacgtcaag
     5341 caatcatgga tgataatttg cttgaattta gagaagcctt ttttgaagaa tacgggttca
     5401 ataaagaaaa cgcaaaaagc ttctaaagga aaatctttta aaaattaagt aaaaaactag
     5461 tgttatcagc atttttctgc taaagtgttt tttgtagata cagaattagt gaggtgaaaa
     5521 aaatgggaac attaccaatg cttgtgatgt ttatcgcgct attagcaatg tggtttttta
     5581 tgtcacgttc gcaaaagaaa caacagcaag aacgtcaaaa cttgttagat agcatgaaac
     5641 caggtgattc agtcgtaacg atcggcggat tacatggagt tgtttcagaa atcgacaatg
     5701 acaaacggac agttgtttta gactgcgaag ggatcttcct tgagtacgat cgtgcagcaa
     5761 tcaaaacggt caaaccaggg actgtcgtaa caaatgatgc aacgactact gttgaaacaa
     5821 cagaagaacc agctgagaca gttgaagtaa aaactgaaac aacagaaacg ctggaagaga
     5881 cagaagagaa ggaagaaaaa taagtatcaa agcttattta aagacgaggt agggcagaaa
     5941 gatgtcctac ctcatttttt attcaaaaaa gaaagagata agagcatggg atatattgct
     6001 caatattgaa taatcgggtg tgtcatataa ataataataa ataactttat atatttttct
     6061 atacaaacac ctgtaaataa agcaatttta tatttaaaaa taactgtgaa aaaaatcaca
     6121 aaaaagtgtt tacatagaaa aataaatatt gtatgatata catgtgaatt atttaacaaa
     6181 taaccatgac ccattaggag gataatgatg gctaaagtaa tagaaaaaga aaaaacgaag
     6241 acaattgacg tacaagcaat gattgatgat ttggcagaaa aagcaaatgc agcattgaaa
     6301 gaaatggaaa gctttgatca agaaaaagta gaccatatcg tgcatgaaat ggcaatggca
     6361 gctttggatc aacatatgcc attagcgaag atggctgtag aggaaacagg ccgcggtgtt
     6421 tacgaagata aagcaatcaa aaatatgtat gcgtccgaat atatttggca taatatcaaa
     6481 catgataaaa cagttggtgt gatcaatgaa gatgtccaaa aagggttgat tgaaattgct
     6541 gaaccagtcg gtgtggtttg cggagtaact ccaacaacga atccgacttc cacaacgatt
     6601 ttcaaatcaa tgatcgcgtt gaaaacaaga aatccaatcg tttttgcttt ccatccaagt
     6661 gcccaaaaat cttctgcaga agcagcacga gtggtacgtg atgctgctat cgctgccgga
     6721 gctcctgaaa attgtatcca atggatcgaa catccttcga tcgaagcgac ttctatgcta
     6781 atgaaccatc caggtatcgc gatcgtctta gcgacaggtg gagctggaat ggtcaagtct
     6841 gcttactcta caggaaaacc agctttaggt gtaggtccag gtaacgtacc agcttatatt
     6901 gaaaaaacag caaaaattaa acgtgcagtg aacgatttga tcgtttcgaa aacatttgat
     6961 aatggaatga tttgtgcttc tgaacaagct gtgatcgtgg ataaagaaat ctatgcagca
     7021 gtaaaagcag aattccaagc acaccaagtt tatatcgtaa aaccagatga attgcaaaaa
     7081 ttagaagatg cagtgatgaa cgaaggaaaa tatgcggtca atccatcaat cgttggacat
     7141 tctgcgatgg aaatcgcaaa attggcagga atcagtgtac caaaaggaac gaaaatgctg
     7201 attgctgaat tggaaggcgt aggcccagat tatccgctat caagagaaaa actttctcca
     7261 gttcttgcca tgatcaaagc taacaacacg gaccatgcgt ttgatttgtg tgaaggtatg
     7321 ttgaatcttg gaggattagg acatacagca gttatccatt cagaaaacga agagttgcat
     7381 gtcaaattcg gtcttcgaat gaaagcttgt cgtattttag tgaatacacc atctgcagaa
     7441 ggcggcatcg gtgatatcta taacgaaatg attccgtcat tgactttggg atgcggttct
     7501 tatggtaaga actctgtttc tcgtaacgtc tctgctgtaa acttaatcaa tgtcaaaact
     7561 gtagcgaaac ggagaaataa catgcaatgg ttcaaattac cgccaaaaat cttctttgaa
     7621 aagaactctc tattgtactt agagaaaatg gaaaacgttg aacgtgtcat gatcgtttgt
     7681 gaccctggta tggtccaatt cggttactgc gatacagttc gtgaagtatt ggcacgacgt
     7741 aaaaatgatg tgaagatcga agtcttctca gatgttgaac cgaacccatc gacaaatacc
     7801 gtatacgcag gaacgaaact gatggcagac ttcaagccag atacagtaat cgctcttgga
     7861 ggcggttctg caatggacgc agctaaggga atgtggatgt tctatgaaca cccagacaca
     7921 tctttctttg gtgcaaaaca aaaattcttg gatatccgta aacggacata taagatcgac
     7981 aaaccagaaa aaacacaatt cgtatgtatc ccgacaacat cagggactgg ttctgaagta
     8041 acgccatttg cggtcatcac tgacagtgaa acacatgtga aatatccgtt agcagattat
     8101 gcattgacgc cagatgtcgc aatcgtcgat cctcaattcg tcatgtcagt tccagcatct
     8161 gtcacagcag ataccgggat ggatgtgtta acacatgcaa tcgaatccta tgtttcagtc
     8221 atggcttcag attatacacg tggattgagc ctacaagcaa tcaagttagt ctttgactat
     8281 ttagaaaaat cagtaaaaac acctgatatg gaatcacgtg aaaaaatgca caacgcttct
     8341 acaatggctg gtatggcgtt tgctaacgca ttcctaggta tttgtcactc agtcgcacat
     8401 aaaatcggcg gggaatacgg gatccctcat ggacggacaa atgcgattct tttaccgcat
     8461 attatccgct ataatgcaaa agatccacaa aaacatgcga tgttccctaa atatgactat
     8521 ttccgtgcag atacagatta cgcagatatc gcaaaattct taggtctgaa aggcgaaaca
     8581 acagaggaat tagtcgaagc tttagcaacg gctgtttatg aattaggaaa atctgtcggc
     8641 atcaacatga gtctaaaagc tcaaggagtc acacaagaaa cactagatac aaccgtagac
     8701 cgtatggctg aattagctta cgaagaccaa tgtacgacag ctaatccgaa agaaccattg
     8761 atctctgaat tgaaacagat catcattgat gcttacaacg gataagcaaa agaaagcgta
     8821 gaaaaaagat aagaaaatag atgaaaaaga acttggaaaa cgttgtttct ccaagttctt
     8881 tttttaattt ccaagtaact gaaagttact tttgttattt aacatttagg tcaattttag
     8941 acagtttttg ttcaaaaagc gatatttata gtggacggta ttactgctat attaaggata
     9001 taaaatgaaa gcgtttaaaa aggtggcgta aagatgatta ttaaagaaga gatttttgta
     9061 cctaaagaat tagtgaaaga aaaaattgac ctgctcgttg aaaacctgac aacgatcaaa
     9121 gacgataatg gtgaattttt gttggacttt gatggactga aagtagatga caaaagctgg
     9181 acagtgtgga attggccaca aggtgtcggt ctatatggta tttataaaaa ttaccagatg
     9241 acaaaaaatc ctcgcgctta tcaagtggtc aatgaatggt ttgaggatcg tatggaagaa
     9301 ggcgctccgc ccaaaaacgt caatacaatg gcaccgctat tgactatggc ttacttgtat
     9361 gaagacacga aagattcacg ctatttgcct tatttagaac aatgggcaga atgggtcatg
     9421 aatgacatgc ctcggacaaa tgaagacggg ttacagcatg ccacatatgg accggaaaat
     9481 aagaatcagt tgtgggacga tacattgatg atgacagtgc ttcctttggc aaaaatcggg
     9541 aaactgttga atcgcccaga atatttagaa gaagctcgtt accagtttat gatccatatc
     9601 aaatacttga tggataaacg aacaggattg tggtatcacg gctggacgtt tgaaggaaat
     9661 cataactatg ccgaagcatt ttgggctcgt ggaaactgct ggttgacgat tgctattcca
     9721 gagatcattg aaatcttaga attatcgaaa gaagatgcat tgcgcaaatt attgatcgaa
     9781 acacttgaag cacaagtgcg tgcattaaaa acttatcagt ctgaaagcgg tttatggcat
     9841 acgttgttgg atgatccttc ttcttacgta gaatcttctg caactgcagg atttgcttac
     9901 ggtattttga aagcggtcca taaacgttat ttaccacaag aatataaaga agtcgcttat
     9961 cgtgctatcc aaggattgct tgaacaaatc gatgaaaaag gagaagttca aaacgtttca
    10021 atcgggacag gaatggggga cagtttagat ttttatcgga atatcggaat cacagcaatg
    10081 ccttatggac agtcgttaac ggtgctttgt ttgacagaat tgctcgtctc ttattgttaa
    10141 aagaaaggaa acccatatga aaaaaacatc actgctgttt gcagcaagtt cgcttagcct
    10201 aggtctgcta ggtctaagcg gctgcggaaa taattctagt gtggcaactg tcgcagatgc
    10261 agatgtaaat gagatagtta ccttgcgctt tgcttatgca agcaacagcc agccagtaat
    10321 tgatgcaatg aatgaatttg ggcgtttagt aaaagaaaaa acagatggtt ccgtacagat
    10381 ccagtatttc ccagatggac aactgggtgg agaacgtgaa ctgatcgaat tgacacagtc
    10441 aggagcagta gatttcacaa aagtaagtgc ctcagcgttg gaaagtttct ctaaagatta
    10501 ttcgattttt tcagttcctt atctattcaa tgatgaagaa catttcttta aggtgatgga
    10561 taatgaagaa ataatggagc caatctacca atcgaccaaa aatttaggat ttaccggatt
    10621 gacctattat gattctggtc agcgcagctt ttatatggta gacggtccga tccatacgcc
    10681 ggaagatttg gagggaaaga agatccgtgt gatgcaaagc gaaaccgcta tcaaaatggt
    10741 agagctttta ggcggttcgc ccgtacctat gggaagtgat gaagtgtaca cgtctcttca
    10801 atccaatttg atcaacggat cagaaaacaa tgaatttgtc ttgtatacag ctggacatgg
    10861 tggtgtggct aaatactatt cctatgatga gcatacacgt gtgccagata tcatcatcat
    10921 gaatgatgcc atcaaagaac agctaactga tgagcaggaa aaagccattg aagaagcagc
    10981 gaaagaatca acggtttttg aaattgaagc ttttgcaaaa gcaattgaag aagaaaaagc
    11041 tttagcacaa gaagaacatg gagtccaatt caatgatgta gataatacac cgtttcgaga
    11101 ggcagttgcc ccacttcatg atatattcaa aaacgatcct acctacaaag acctttatca
    11161 aaagatccaa gagcaaggag cgtagctgtg atgaaaatga ttttaaacaa ggtattagag
    11221 atcctgggag ctgttttgct tgttgggatg gtcgctgttg ttttatggca agtattttcc
    11281 agaacggttt taggaaatcc aaatacggtc acggaagagc ttgttcgatt cggtcttgtc
    11341 tggtttgcca tgttgtcttc cgcgtatgtt gtgggacaaa aagcacattt agccgtaacg
    11401 gttttaagtg aaaaattaac tggcaaaaaa agtaaaattt tagaagttgt tgttcaaagt
    11461 ttatttctgg tatttgctgc gatgatcatg gtgtatggcg gatggaatgc tgtcacattg
    11521 acaatggggc agatctcgcc ttctttaagt attcctatgg gatatgttta tctgtcagta
    11581 cctgtatcgg gggtactcat cattatctat agcctgatca atctaatcga tacattaagc
    11641 aacaagccag caattcaaga aaaaatataa aaatggagga ataaaatggc aatagaagcc
    11701 ggattagtgt tattttttgt atttgttgtg ttgcttgtat tagggatgcc gattgctata
    11761 agtattgcat tctcatcgat gatgacttta ttgttagtta taccgtttga tgtttctgct
    11821 tttagttcag ctcaaaaaat ggtgggaagt atcaatagtt tttcgctgat cgctattccg
    11881 tttttcgttc tctcgggcat tatcatgaat aatggaggga tagctaagaa actagtggat
    11941 tttgcgatgt tgttcgtcgg gcgcgtgccg ggggctttgg ctcatacgaa tgtgttaggg
    12001 aatgctttat ttggttccat ctctagctcg gccattgccg cttctaccgc gattggcgga
    12061 gtgttgatcc ctcagcaagt ggaagaagga tatgaccgga agtttgcgac agcggtcaat
    12121 atcgcgtcag ctccgacagg tatggtcatt cctccaagta ctgcttttat catgtattca
    12181 cttgttgctg gtggtgcttc gatctctgct ttgtttatgg gaggttatct cgtaggcgct
    12241 ttgtgggcgc taggaatcat gctagtagca ttcatcgttg ctaaaaagaa taaataccct
    12301 gttgtcgaaa aaggacagat ggctcatgct ggaaaaataa taaaagaggc gattcctagc
    12361 cttctattga ttgtcatcat tatcggtggt atcttgacgg ggatatttac agcagtagaa
    12421 gcttcagcga tagctgtcat ttactccctg ttgattgcta tgttttacta taaaacggta
    12481 aaactaaaag atatacctaa tatgttgttt caatcagtag caatgtcagg gacgatcatg
    12541 ttcttattgg ctacttcttc catgatgtct ttcgccatgg cgtttaccgg tattcctcag
    12601 gcaattagtt cgttgattat gggcgtaacg gataataaat ttattattct gttattagtc
    12661 aatcttgtgt tattactggt gggtatgttt atggatgtgg cgcctgctat cttgattttt
    12721 acaccaatat ttttaccaat cgtcacaagt gtaggggttg atcctgtaca ttacgggttg
    12781 ttttcaatca tgaatctgtg tgtaggctcc atcacgccgc cagtaggtac aggactatat
    12841 gtcggtgcaa gtgtaggagg cgtaaaagca gaggcaatgc tgaaaccgct tcttcctttt
    12901 tatggggtta tcttagtcgt cttgtttttg attacttact tcccgcaaat cgtcatgtgg
    12961 ctacccaata tgctgagtta ggagaatagt tatgttgaac attacaatta gaggacatga
    13021 tctttccgaa gtccaaacga tagaagatct tgcagaaaaa acaaaagaac aagggattca
    13081 tacactgcag ctcgccttag gtctttcgtt tcctgaaaca ccttctggcg caaatgagat
    13141 caattctggg atgggaaatt atgtcaaacg agttttagaa aagcaagaag tgaccgtagg
    13201 aattttaagt tgctatatca atatgattca tccagattta acgatacgac aagagttgct
    13261 gacaaagttc gagcagtatc ttcgctatgc ttcttccttt ggtgcgtcga tggttgcttc
    13321 ggaaactgga tgtgttttgc cggaaatcca atatacggaa gaaaatttca cagatgaagc
    13381 atttgctgaa gctgtgtccg ttatccgtcg cttggtaaaa gctggagaaa aataccaaat
    13441 gatggtgggg atagaaccag gtttgaatca tccggtgtat tctttagcaa gggtagagca
    13501 gctgatccaa gcagtagatt cggactatct aggaatcatt ttagatccga ccaatttgat
    13561 cacaagtagt gattatcaag aacaagtcca attggtagaa gaagcttttg aacgttttgg
    13621 tgaaaagata tgtgcggttc atttgaaaga ttttcgtgtc gaacaggaaa aaatcgtgcc
    13681 ggtaaatttg ggagacggtg tgatcgagta cacaaaaatc aaagaaatca tcatgaagaa
    13741 tcgtccgtac ctgtatgtgg ttcttgaaga aacaaaagat gatgggattc gttatggaag
    13801 aagtcttctt gaataatgta aagaagacgt tggttaacta atatttagaa tggagggggc
    13861 ggggctagaa gatgccacgt ctcttctttt ttataagagt atataaaagc agctcttttt
    13921 gattacaaaa aacgtggtat aataaaattg tgcataagtg aacaaaagga ggtgcggcac
    13981 atgctccaaa agaaaatcag cggtgaacaa gtcaatacga tggtttctga tcagtatgaa
    14041 atctatcatg tcaaagatgt cgtcagccaa aataaaacga tctatcatca tcatgatttc
    14101 tatgagatcc atgctacatt aaagggagaa gcgacttttt atttgaacgg acgacaattt
    14161 acgatcaaag caggaagtgt gctgctcatc cattccaatg atttgcatcg tatcgtccgc
    14221 caatcttcag atgtattcga gcgtgtctat atctttgtca ccccttcttt tctagaaagt
    14281 cgttccacgc catggtctaa tctatcggct tgttttcaac caatgggaga gaagaggagt
    14341 cgtgtattgc aaatagaaca ggaagtattg aagcaaaaat tatcatttat cgaccatcct
    14401 cctgataaaa aaatttacgg tgaagacata aaatatgaac aagctctggt agaatacttg
    14461 atttttctca atcaagttgt acaaaaagaa gagagtgcgc aagaacagaa tgctgtagtt
    14521 cctaatgagc gtgtggaaca aatgttacaa tatatctcaa aaaatttatc tcatccgctt
    14581 tctttagagg aaatggaaaa gcagtttttt gtcagcaaat accatatcac acgagaattt
    14641 aaaaagtaca ctggatttac gtttcatcag tatgtaatga agaaaaagct gatctatgcc
    14701 aagcacttgt tgcgagaata ccgcagctct agtgctgtat atagtaaaag tggtttttct
    14761 tcttatccgc attttctgaa agcattcaag agagagttcg gcataacgcc taaagaattt
    14821 ttgaaacgtg atacacagaa acagttggtt cattatgaac attttgaaga tgaacaatga
    14881 gaatgatcac taaaaaataa ccgcagtagc tgctgttgtt tacatagaag cagaaactgc
    14941 ggttatttta tttttatcta tatatttcaa aatattttga aaaaatgacg tacatattat
    15001 atgaatcagc tgaatttacg aaagtctctc ggcgatagtc ctgtttttgc tttgaacagg
    15061 cggctaaagt aagcaaaatt ctcaatccct atttcttcgg aaatcattcg aaccgtaaat
    15121 tctgtatacc gaagaaggtc tttagcgtaa gaaagttttt tgctgatcaa ataatcgatt
    15181 ggtggagaac cgataaattt ggaaaaatct ttgctcagct gatatttatt caataagaat
    15241 tgttgttcta attgatcaag tgtaatcgtt tctttgaaat gttcgtctaa gtattgcttc
    15301 agtttgtaaa cgtaggcggg aatatcctct tcttcgaaat ctagctgata cttttgcaaa
    15361 atcagttcat tcagcaactc gtggatagct acagaggact ggaaatcggt acgggcattc
    15421 gtgccttgtt gcatgtggat caattctgtg atcaaatggt ggatcttgtt ttctgatgga
    15481 tcccctgttg aatggaaggt gttatggccg tcttttgtga actcctgata aaatgattgg
    15541 acgttctgac cattcaaatg gatccaatcc atttcccatg gttcttcgct gatcgtttga
    15601 taacgctgat attcttggca gtcaataaaa aataagtctc cagcttttag ctgttgctga
    15661 ttatcattat atgtaagcga acctttgcca gacaaagtaa actttatcaa gtaagaaggt
    15721 aaatgtgctc gttcagtata gtaagggatt ggtgctttga agtaaccgat ttcttggata
    15781 taaaaaaata aattcctgct tctttgtgta ggcgtcaaga tcactctttt tgagtcttcc
    15841 gaccatgagt ggttggcttt ttcccagtaa tccatgtaaa cactcccaac ttgtcaagat
    15901 aatgcaatta tataccaaaa tagtgtgaag caaaatataa gattctgtat tatgatagca
    15961 ttgttaataa atgataacgc tttattaata aagtgctaat caacaataaa gtaaaagagg
    16021 tggagaaatg aatccaacat ttgttgccat cgatatcggt gcatcaagcg gacgactaat
    16081 gaaaagtacg agggatgcaa aaggttcttt gatcttggaa gaagtacacc ggttcaaaaa
    16141 cggctttcgg aacaaagacg gtcacgatcg ctggaagatc gattatctta tcaaagagat
    16201 tttgattgga tttgaaaaat taaagcagac agggattgat cgcctttttg cggggatcga
    16261 tacatgggca gtcgattact gtctgattga tgaacaaggg gatcggataa atgatccgat
    16321 tgcttatcgg gacaagcgta ccaatggagc agtagaacag ttttctgaaa tctattctct
    16381 agataagttg tatgagcaga cagggattca gatccagcct ttcaatacct tgttccaatt
    16441 gtttgctgaa gaaaaagaaa atttggcaaa agcacataaa ttgttactga tacctgatta
    16501 tttagggtat gtctttactg gtaacatggt gacggagaaa actaatgcat ctactatgca
    16561 gctgctgaat gcagggacaa aagaatggga aatagaactt cttgaaaaaa tcggtgtctc
    16621 gaaggaatta tttccaccat tgacagagcc gggaacgata ctaggagatc tgcaaaaaga
    16681 acaattttca aaatatgatt tgccagatgt gacctttatc acagtagcaa gtcatgatac
    16741 ggcgtcagct gtattaggaa cgccaggtca aggagaacgt tggggttata tcagcagtgg
    16801 gacatggtcg cttttaggga ttgaaacaaa gatcgccaat gtttctcgca atgctttttc
    16861 tgaaaattat acgaatgaat ggggtgccca taacacggtg cgttttttga aaaacatcat
    16921 ggggatgtgg ttgatccagg aagttgcccg aatgcaagag tacgagtaca gttatgcgga
    16981 attggcagag cttgctgcaa aagaaaaacc gctgcagcaa gtgatggaaa tcaatgaccc
    17041 tcgatttttg aatcccccaa atatgataga agagattcag caatactgtt ttgaaacaga
    17101 gcagccagtc cctgtctcat cgggagctat cgctcgttgt gtgtatgata atcttgctct
    17161 ttgttacgca caagagctga tcaaagtaga acacttaact ggaacgaaat tggaaaagct
    17221 gcacatcgta gggggcggat cgaataatcg tctactgaat caattgacag cagattatgc
    17281 cggaatcatt gtagaagcag gacctagtga agccacagca atcgggaatg ttgccatgca
    17341 aatgctcagt acaggtcagt tttcgaatat agaacaagca agaaatgtaa tcaaacagtc
    17401 gtttccatgt gaaacttttt acccagatga aaataagcaa aacacgatgg aatggtacgt
    17461 gaataaaatc aaaggagtaa tcgtatgaac acgattgaaa gaaattacga acaagcaaaa
    17521 gaaaaatatg caactatcgg tgtagataca gatgctgtac tagaaaagat acaggacatc
    17581 aaaatttcta tgcattgctg gcaaggagat gatgtcaaag gtttcttgac accggatggc
    17641 gaattgaccg gtggaatcat ggcgacaggg aattttccag gagccgcacg aacacctgaa
    17701 gagcttagac aagatctgga gaaggcttat tcgttgatac cgggcaaaca caaattgaac
    17761 cttcatgcta tctatttgga tacagaagaa tctgtcgatc taaatgaaat tgagccgaaa
    17821 cattttgaaa aatgggtaga gtgggcaaaa aaagaagaaa tcggtcttga cttcaatccg
    17881 actttctttt cgcatccaat gatgaaagac ggctttacat tggctcatcc agataaagaa
    17941 gttcgtgaat actggatcga acacgggaaa agaagccgca aaatcgcgga gtactttggg
    18001 aaagaaactg ggcaaacaag tatcaataac ttttgggtac ctgatgggat gaaagataat
    18061 ccgattgatc gttttacccc acgggaacgt ttaatggaat cactcgatga aatttttaca
    18121 gaagagttag atgaacggta tgtacaagat gcagtagaaa gcaaactatt tggtttaggc
    18181 gctgaagctt atacagtcgg ttcccatgaa ttttatatgg gttacgggtt gactcgtgac
    18241 aaacttgttt gtctagatgc tggacatttt catccaacag aagtgatttc caataaatta
    18301 tcttcattag ctttattcag caaaggaatc atgttgcatg tttcccgtcc agtgcgttgg
    18361 gattcagacc atgtggtttt gatggatgat gaattgcagg aaatcgctaa agaattagtc
    18421 cgtaacgaat tgttggaaaa aacaaatatc ggtctggatt tctttgatgc aacgatcaac
    18481 cgtatcgctg cttgggtcat cggtacgaga aatacacaaa aagcactgct gaaagctatg
    18541 ctggaaccag ttgagcgatt gaaagaaatg gagctggctt ttgactttac ttcaagaatg
    18601 gcttatacgg aagaattgaa agatttcccg tatgccgatg tatggaatta tttctgttac
    18661 aaaaatcaag ttccagttgg tttggattgg ctggaagaag tccaggaata tcaaaaagac
    18721 gtgttggaat taagaaaata ggagtgtggc tatattgaaa aaaatagata ttttacaagc
    18781 gccttatgtg aaagaaatga tcgaaacgac gtcgaatctt tatcgcttgg gatgggatga
    18841 aagaaatggc gggaatattt cttatttact aaaagaagaa gagatcacac cgttcttgga
    18901 tcctgaaaaa gtgatccgcc gcatctcgat gatttttgat gccagccgtt tagcaggatg
    18961 ctattttatt gtaactggtt caggaagata ttttaaaaat gtagcgagcg aacctgccga
    19021 gaatcttgga atcgttcgag ttgctgaaga tggcaaaaca ctggaacttt tgtgggggct
    19081 ggaaaacgaa tgccttccta caagcgagtt gccaagtcat ttcatgagtc atatcgcccg
    19141 tttagaagta gacccagata atcggatcgt tatgcattgc catgctagtc atctattagc
    19201 gatgagtttt acgcatgaat tagacgaacg ttcctttagt cgtacgctat ggcaaatgtg
    19261 tacggaatgc ttagttgttt ttccagaagg aatttccatc attccgtgga tggttccggg
    19321 aacaaatgag attggggaag caacagctga aaaaatgaaa gaaactcgat tggttttatg
    19381 gccacaacat ggaatctacg ggtcaggaaa agacatggat gaagtctttg gtttgatcga
    19441 gacagcagaa aaggcagcag aagtctatac gtacgtcaaa gcgcaaggac caatcttgca
    19501 aacaatcacg gacgaaaacc tgtggcattt agcagatgcg tttggtgtca ctccaaaagc
    19561 aggttatttg gaggaagttc atacgaaagc aggtgtctga catggtgaaa aaagcttttt
    19621 gcatgaaagt ttacccagaa aaacaagaag aatacaaaaa aagacatgat gaactttggc
    19681 cagaaatgcg tacagcactt cgtgaacacg gggtcatcga ctattcgatc tatcttgatc
    19741 cgagtacaag tttgttattt ggttacttag tgatcgaaga tgaaaagtta tgggaaaaaa
    19801 tggcaaatac atctatcaat caaaaatggt ggaagtacat ggaagatatc atggagacaa
    19861 atccagatgc ctctccagtc agttgggaat tgatgccagt ctttgaactg tagcaacaag
    19921 caactgggca accggttgct tggggttctt tcgtttttat tgtgaaaaaa aatacaaatt
    19981 aggaggaata gaaaatgagt aatcgaatga ttttaaatga aacatcgtat tttggaaaag
    20041 gagcgattga gtcgatcgtc cctgaatttc agcagcgagg attcggcaaa gcagctgtca
    20101 ttactgacaa aggattgatc gagcacggta tagcaacaaa agtaacagaa cttctagata
    20161 aagcagcaat cgcttatgct ttatacgacg ggatcgtacc taatccaacg atccaaaatg
    20221 taaaagatgg agtggctttt gttaaagagg cagaggcaga ctgtttgatc gcaatcggtg
    20281 gaggttcgcc aatcgatacg gcaaaagcca ttgggattat tttgacgaat ccagagtttt
    20341 cagacgtcat tagtttagaa ggtgttgctg atacgaaaaa tccatgttta cctatattag
    20401 ctataccaac gacttctgga acagcagcag aagtaacgat caactacgtc atcacagatg
    20461 aagtaaatca ccgaaaattt gtttgtgttg atcctcatga tatcccaatc gtcgcgttca
    20521 tcgacagcga tatgatgatg ggaatgccga aaaaattggc tgcatcaaca ggtatggatg
    20581 ctatgacgca tgctattgaa gggtttatca caaaaggcgc ttgggaaatg actgatatgc
    20641 tgcatttaaa agcaatcgaa atcatcggcc attctttgga agtttctgta gatggcgatc
    20701 aaaatgggcg agaaaaaatg gcattaggac agtatattgc gggaatgggc ttctcaaatg
    20761 tcggtttagg actggtacac gggatggctc acccgttaag tgcatggtac aacattcctc
    20821 atggcgtagc gtgtgcagca cttttgccaa ctgtcatgaa atataacaaa gaatatactg
    20881 gagaaaagta ccgtgagatc gctcttgttc tagggattaa aggagcagct gaaatgtctt
    20941 tagaagatgt aagagaagcg gcttgtggtg agatcgatcg gttgagcaaa gcggttggaa
    21001 tcccagaaac catttctgaa ttaggtgtaa aagaagcaga tattccagca attgcagaag
    21061 atgctttgcg agatgtatgc acaccaggaa atccaagaga aacgacagta gaagaaatca
    21121 ttgctttata tcaaagtttg atgtaaaaac gaagcggatc ttcaacatta aattgcctgt
    21181 aaagagaaat ggtagtttat atctggatat ctcttttaaa ttgatggtat ttccgctata
    21241 atttttctta taaagaaata ggagaatatt atatggaaaa taaaaagcct tatggaactg
    21301 tattgatcaa agcctctaaa atactagact atttagcgga aaaccaagat gtctctttgc
    21361 agacgatcgc aaagggtgtg ggaatgacct cttctacagt cttgaaaata ctagatactt
    21421 tgttgatgat cggctacgta aataaaaatt cagagaaaaa ctatcgttta ggtgcaaagc
    21481 ttattcgcta cgcgaataaa aacattgaac aaatcgattt ggtagagttg actttaccgt
    21541 ttttagaaca gctgcagcaa aagatcgatg agactatcca cttaggggta ttggacaaca
    21601 atgagatctt gtacgtgaac aaactagatc caaaacatca aacgatccgc atgtcttcaa
    21661 aaatcggaat cacccgacca ttatacaatt ctgcgatggg caaagccgta ttagcagaat
    21721 tcagtgaaga gcagtacact gattatcttg aaaagcatcc gttgatccct tacacagaat
    21781 atacgatcac caatgcatta cggttgaaaa aggaaatcca aacggtaaaa gaaacccaca
    21841 tcgcatttga cgatgaagaa atcgaacgtg atattttttg tataggagcc tcaatcgtga
    21901 aaaacgaaca aatcattggt gcatttagtg taagtatgcc gaaatatcgt ttaacagatg
    21961 agacaaaaga acaaataatc agtgccctca agcaaacaaa aagcgaaatc gagcaaagaa
    22021 ttcaaaaata atgaaaaaag cgtttgcaat ttttcttttc tgccgtataa tatgggtata
    22081 gatttccaag ttataaaata aaatttctaa ataggaaata ggtggagaag atgaaaaaaa
    22141 ttgatatttt atctcgattg aaaaacgccg gagtgattgc ggtggtacgc ggaaaaagca
    22201 aagaagaagc tttgaatgct tgccacgcaa tcatcaaagg tggtctaact ggtattgagt
    22261 tgacttttac agtccctcaa gcagatcaag taatcaaaga acttttgtca ttctacaaag
    22321 accaaccaga aatagtgatt ggcgcaggca cagtattaga cgctgtcaca gctcgcttgg
    22381 ctatcttagc tggcgcagag tatattgtta gcccttcctt tgatcaagaa acggcagaga
    22441 tgtgcaatct gtatcaaatt ccttacttac caggatgtat gactattacc gagatcaaaa
    22501 cagcgctgaa aagcggggta gatatcgtca aactatttcc tggaagcgct tatggaccaa
    22561 gtatcatttc tgccttcaag gcgccgatgc ctcaagtcaa tatcatgcca actggcggtg
    22621 tgagtctgga caatatgaaa gagtggttcg atgctggtgt cgtaacagtc ggagtaggtg
    22681 ggaacctttt agcacccgca gctacaggag attttgacaa agtaacggaa gtcgcgcaac
    22741 agtatgcagc aaaaatgaaa gaaatcaaga ggtagctgct tatgggaaaa gtagtgacac
    22801 tcggagaaat catgttgcga ttgtccacag agcagggaac ccgtatcgat tcaagtgaac
    22861 gattcgctgc ccattatggc ggaggggaag caaacgtagc aatttcttta gctaattacg
    22921 gacatgaagc gatttttgct acaaaagtac ctgataacgc attgggaaaa gcagtcaaaa
    22981 agcacttgca atgttatgga gtagatacga aacaggtatt atctggtgga tcaagactag
    23041 gtacttatta tatggaaact ggtagtggcg aacgcgccgc ctcagtgatc tatgatcgtg
    23101 ccggatcaag ttttgctgaa atggatcatt tggaatggtc gctagaggag ttattccaag
    23161 gaatagatat atttcatgtt tcaggaatca ccccagcttt gtctaaaaaa tggcaaaaaa
    23221 tgacgaaaca ggtgatagaa gctgcaaaaa aagctggctg cctgatcagc ttcgatatca
    23281 actatcgtgg aaaattatgg acacagcaag aagccagcga agtaatccat cagcttttac
    23341 ctttagtaga tatctgctca gctggaaaaa tggatgctct ttatttgtta ggtatcgaga
    23401 aagcgccaga agcagaagaa caacctctaa tttattatta tcaaaaaatg caggaaaagt
    23461 tcccgaatat tcaggtgttt tattcgacca agcgtaccgt tcattcagca agtgaaaatg
    23521 aattgaaggg gacattgtgg atgaaccatg aatatgtgga atcccaagtc catagaatca
    23581 ccccaatcgt ggatcgagta ggtggcggag atgcttttgc aggtggcgtg ctccatggga
    23641 ttttatccaa tatgtctccg caagaaatca ttgattttgc tacggcagct tctgccttga
    23701 agcatacgat ccatggggac tgcaaccaat ttagtcaaga agaagtcgca caatttttag
    23761 catgcggctc tggaaaaatc attcgataga aggagtagaa gaaaatgcaa gaaatggaaa
    23821 cacgttatac acacagtcca gaagatatcc gtcattattc aacggaacaa ttgagaaaag
    23881 aatttttagt agaaaaggta tttgaaccag ggaaaatcag tttgacttat acgcacaacg
    23941 atcgaatgat ctttggtgga gtgacaccta ctgaaaaaga attagaaatc acattagaca
    24001 aagaactagg tgttgattac ttcttagagc gtcgtgaact aggtgtgatc aatatcggtg
    24061 gaccaggatt catcgagatc gatgggcaaa aagaagaaat gaaaaaacaa gacggatact
    24121 atatcggaaa agaaacacga catgttgtat tttcttctca agatgctgaa aacccagcta
    24181 aattctatat tagttctgtt ccagctcatc ataaatatcc aaatgtaaaa atcagtatcg
    24241 accaagtaaa accaatggag actggtgaag gcctgacact gaatgaacgt aaaatctacc
    24301 aatacatcca tccaaatgta tgcgaaagct gtcaattaca aatgggttat acgattttag
    24361 aaccaggaag cgcttggaat acgatgcctt gtcatacaca tgaacgtcgt atggaagctt
    24421 atgtctactt tgattatgcc aatgaagata cacgtgtgtt ccacatgatg ggcaaaccag
    24481 atgaaacaaa acatttagtt gtagacaatg aacaagccat tatttcgcca agctggtcga
    24541 tccattcagg tgtaggtaca agcaattact cattcatctg ggcaatgtgc ggagaaaata
    24601 tcacctatac agacatggat atggtgcaaa tggatcaatt aaaataagca taagggagag
    24661 aaaatgatgg aatttagtat ggatatgttt cgtttagatg gaaaagtagc attagtcacc
    24721 ggtgctgtct atggtattgg atttgagatt gcacgctctt tagctacagc aggagcaaaa
    24781 atcgtattta ataacttgaa tcaagaatca gttgatgaag gtatagcgca ttataaagaa
    24841 gcaggcatcg atgcaaaagg ttatgtctgc gatgtgaccg atgaagaagc ggttcaagcg
    24901 atggttcaac agatcaaaga agatgtaggt tcagttgata ttctagtcaa caatgcaggg
    24961 atcatcaaac ggacaccgat gatcgaaatg gatgcggcag acttccgcca agtaatcgac
    25021 gtggatttaa atgccccatt catcgtttca aaagcagtta ttccggatat gatcgaaaaa
    25081 ggcggcggta aaatcatcaa tatctgctcc atgatgagtg aattaggacg cgaaacggtc
    25141 agtgcttatg ctgcggcaaa aggcggatta aaaatgctga cgaaaaacat cgcttctgaa
    25201 tacggtcaat acaatatcca atgtaatgga attggaccag gttatattgc tacaccgcaa
    25261 actgctccat tacgcgaaac acaagaaaac ggtgaacgtc atccattcga tcaatttatc
    25321 gttggacgta cgccagcagc acgctggggc gagccaatcg atttggctgg accatctgtc
    25381 ttcttggctt ctagcgcatc tgatttcgtt aacggccatg tcttgtatgt ggacggaggc
    25441 atacttgcct atatcggaaa acaaccttaa aagagcttta gtacactgtt tagtggagag
    25501 gttgtgaaat cagcggattg aacgaatacc gaaggacagc tgattaaaca tcatttattc
    25561 gaaaaaagag gctgggacag aagcatttaa ctccaagaaa taagaaggaa ttcacgaaaa
    25621 ttgcttttca aatttttgtg aatttcagct tatttccgaa ggagttgctt ttacttccgc
    25681 cgtttgtacg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt
    25741 tctgtgcgtc ttccatactt cactaagtcg atccctcttg cttcgcacca atccttcact
    25801 ggtccggaaa agatcagaga ttgttggtgt agggccgcgg tattcgttgc gccgaccata
    25861 gtataaagca gacgaagctc ttcttgccat tgcttcatca gtataatagt ttcttctacg
    25921 ccgtgattca tcagatgcgt cagtacggtt cctgaagtac ccacggcatt tgcgccgaga
    25981 caaagggctt taaatatgtc ataggcattg cggatacctc cggaggctag gatttccata
    26041 gaagtgtctg cttcatttgc ttcaagcaat gaagagactg tcgattgccc ccagtcagct
    26101 aaataactca gttcccgctt ggagcgacgg gcgttttcga tctgggtgaa gcttgttccg
    26161 cttcgaccgc tgatatcaac tgtatgaact cctaaagcag ctagatcatt gagcgtttct
    26221 cttgtcatgc caaaaccgac ttcttttacg atgaggggga cgtcaatggc tgtttgagtt
    26281 tcttgaataa gtactttcca attggtaaaa tcacggtctc cctcaggcat aaccaactct
    26341 tgaggtgcat tcaaatgaat ctgtaaggca tctgcgtgaa acaatcggat agcttctttg
    26401 gctctttcga cagaagtccc tgcaccgatg ttggcaatga ttttcccgtg aggatattct
    26461 tgtcgcataa tcgtataagt atcggctaaa gaagggactt tcaaggcagc gctgactgaa
    26521 ccagtggcaa tcatcaaatc tgcttctctt gcgacgattg caagatcttg gttgattttt
    26581 tttgtttttt cgcttccgcc agtcatcgca ttgatataaa atgggctgct tagtgtaaac
    26641 ccaactgcct gtgtacttag atcgacatca gaaactgcta tttgcggcaa agggttatgg
    26701 atgatccgta cgaagtcaaa ttcgtttttt tgtttatcat gaaaggcttt agctagtgaa
    26761 acatgttcgt cttttcgatt catcggtttt cctttctttg atcgctatag acatgtaacg
    26821 gcagtggagt gatttctgct ttttcccatg cactcattaa aggaagaatg ccagatttct
    26881 ggtcaacaat tacgattccg caatcgcccc cacctgcacc agaagatttt gcggctcctt
    26941 catactgttc agctaaatta cacaatttgt tcaaagcagg cgtttcgatg acgaccccag
    27001 tgattgcaga taaatcatgc agtaattgtc ggttttttcg aatcatctgt tggatcaacg
    27061 ttacattatt ttctttaaac cctttgatca tttcgttgac acattctgta ctatttttta
    27121 agaaaagctg ataagccacc attttatctt ctctcgaacg gtgaacttga tcgaccaaat
    27181 cagaagtaga ggcagggcta cccgtccaac caatcaataa acgtaaatct tcaggagcaa
    27241 tcaatggctc aatggataga cctggccaat ccagggcaag taactcactg atagaatgct
    27301 gagtttcttg ttcttggagc caaggatggt cgaaggttga gaaagcgatc cagccaccat
    27361 aacagctagc agcgatgtcg ccgcaggaac cattatcttg aactgctaaa ttggctagtg
    27421 ctgcaatctt gaaaatctcc aactgagata aattcaacgc gtaaaaaaca ttcaaggctt
    27481 tgaccgttgc aacggtcacg gcaccgctag aacccaatcc atatttccgg ccatttgaac
    27541 tgtctaactc acttgttact ttcagatcat aaaaggataa aagaatgttt ttttcttgtg
    27601 catacttttc agtcaagcga atagcagcaa ggatataatg aaaaggattt tcccgaatat
    27661 ctaaaactaa ttctccgttg cgtcttgtcc aacgtacagg catcccacta tattgtgcag
    27721 attggatact tccgactttt cgtgcagatt ctacagtgac tgtcacgaat tgatcgactg
    27781 cagcgataac tgctggatgg cctgtttcaa caactgcata ttctccggca atatagagtt
    27841 tgcctggtgc agatacttca atcataaaaa tctcccttta ttcaataatc gcaattcctg
    27901 gaccagcatg tgcagtaatt acttgttctt tgctaaataa tttggtaaat gtttcttgta
    27961 cctcagataa atggctgttt tccactaaaa ctttgacatt agggccagca tccatcgtaa
    28021 agtagcatgg aatgccttgc tttctcaatt gtcgcaccgc atccatcgct tttaagctgt
    28081 caggagacca gtaagtgaat ggaggctggg cagctaaagt cgttccatgc atttttagtc
    28141 cgtttctttc catcgtttct ccgagaagtt ggaaatcttt tgttttgatt gcttgtttca
    28201 attgatatag atccccttca acggaatcta accagccttg ataaaaactg gatgtttcga
    28261 ctgtccgacg catcccattt ctgctggaca cttctttttt ctggtcgttg atcaaaacga
    28321 aaaccattgc taaatcgtct tcgaaagaat cggaagggac tggcttagcg taagaactta
    28381 agtcgtcatg gcctttttcc cattcgacga aaccaccgaa aatacttcgg caagctgaac
    28441 cagacccgcg tcgagcaaat cttgaaagag agagatcatc taatccaagc ttaagagcag
    28501 tattgcaagc gcctgctaaa gcagctagcc cgctggcaga ggaggctaat ccagctgcgg
    28561 taggaacgaa attctgactg attactgaag cttttagtga gaggccggct tctttacgaa
    28621 aaaggtcaag aaactgactg actttttttg ttgctttttc gtcttgcaag gtgccatcta
    28681 gataaaattc atctaccata tagctatcag aaaagatgac ttctgtctct gtataaaatg
    28741 catccaatgt taacgaaagg ctgttgttca ttggaaggat gagttcttca tttttcttac
    28801 cccaatattt gattagagca atgttggtat atgcgcgtgc tttgccttta aacataggta
    28861 tgtactccta atccttgtat ccaagtttca gcagcacccg catcttcaag tgcttgactg
    28921 atttcttggg cttttgtttt tgtttgtgct aatgcaatca tacagccacc gcgtccaccg
    28981 ccagttaatt tggcacccaa tgccccggta tcttttgctg tttggatcaa tagattaaga
    29041 aaatcattgc tgattgtcag cttttccaga agtgattgtg attcatccat tgtctgtgcc
    29101 aacgtttctg gtgaattttc aatgattgct tgttttgctt gcttcgtcaa gtatcctaat
    29161 ttttggatca tttgtccagt ttcatgaggc tgggtttcaa agaggtgagc gacatctttg
    29221 acggcttctc ttgtttgtcc tttgattcct gtatcagcga caatcaaaaa agcatcgatg
    29281 ttcaaagaaa agtagtcgaa aggatgccct tttgtaaaat aaatcggctg caagctgcta
    29341 gtagcggctg catcgattcc actaggatta ccgtgggcga ttttttccga aagctggaca
    29401 ttttctaata gtatttcacg agacaaagga aatgctaagt aatcataaaa agcacgagtg
    29461 actgctgtgg cgactgcagc gcttgatccc attccacgtt cagcaggaat cgtgctttca
    29521 atggtcagtt gcaagttttt ttggatgtca tgctgttttt ttaagtgaat gaataatttt
    29581 ttgatatttt ttaatgcatg aggagcttcg gctaaatttc ctgaatagta ggaagaaacc
    29641 agttgatcgt ccattgcatc cagctcttca aggaatgcgg tgacttttgt tgcatagaaa
    29701 ggaaaggcaa tcgctggttc tccataaaca accgcgtgct cgcccatcaa tatgatcttt
    29761 ccgcttgatt ctccttggcc atagtttgcc ataatctaac tccttctcac gtaaaagtga
    29821 tttttaagca aaaaataaag cgcccaaaac gagttgcgct gcttcacgaa tttttttgtc
    29881 cactaagggt ctcaaaaaac attcctcctt tattttacta aaaccatctc agaagtaaaa
    29941 ctaagaatac gttttctaaa gaaaaaacac ttttttctta agaatttttt gacttttttt
    30001 cttctttgac tagaaaaaaa tacaattgac actactagat attgggggta tatttaaaaa
    30061 ggttggagtt aaacacaata tttagtgatg aagtgagctg gaggtaaatg agcgtgatcg
    30121 ttttagcagg tacaattggt gccggaaaat caagtttaac agaaatgatg gctgaacact
    30181 ttgatagtca ggcattttat gaatcgattg atgataatga agtcttgccc ttattttatg
    30241 caaaccctga acagtatgct tttttgctgc aaatttattt tttgaataaa cgttttgcaa
    30301 gcatcaaaca agcaatgaaa gatgacaata atgtattgga ccgttcgatt tatgaagatt
    30361 cactgctttt ccatctaaat gcagatctag gaagagcaac agaaacagaa gttcgtgtgt
    30421 atgatgaact gctggaaaac atgatggaag aacttcccta tgctgcccat aaaaaacatc
    30481 cggatttatt agttcatatt cgtgtatctt ttgatacgat gttggagcgt atcgaaaaac
    30541 gtggtcgttc gtatgaacaa ttgtcctttg atccaagtct atacgattat tacaaagaat
    30601 tgaacagacg ttacgatcaa tggtatgaag aatacaaaga aagtccaaaa atccaaatcg
    30661 atggagaccg ctataatttt gtggaagatc ctcaagcaaa agaagaagtc ttgaaaatga
    30721 tcgaagaaaa attagcagag attcgccaaa ccaaagtagc catttgattt acagttgctc
    30781 aaataaaggg taaaccttta tttgagcgac ttttttcttt atactggaaa caaatgtcag
    30841 gaggatagag tatgcgggaa aaacactatt ttaatcaaga ccgtaatttt tggatcaatg
    30901 tacagcaaaa cgatgtgcta gctgttaaaa acttaaaaga agaatatggc attagtgacg
    30961 agatgctgac ttattctcta gataaaaatg aacgggcgcg cgtagagtac gattcttttg
    31021 atgaagcgtt actgcttgta tcaaatgttc cgcaccagca aaaagtcgat aatcattatg
    31081 aaactagtcc tatcgctttt attttaaaag aagacggttt gtttacattt accacgccga
    31141 ataccgagta cgtgattcga ctaattcgat cgcttttgga acgaatgcca gacatgagtg
    31201 tatacagttt attgtttcgt acattgtttt tgatttctga ttcctttttt cctttaatcg
    31261 aggaagtgaa tagtgaacgt cagcggctta atctgaaatt aagagaaaaa acaacgaaca
    31321 agaatctgct gcaactttca gatttagaga tcggtttggt ttacttagtt acaggaacaa
    31381 aacaaaatgt agtactttta gaacaaatca aggcattggc tatttatcgc aagttgtcag
    31441 aaaaggaaaa agaagagttg gacgatgcgc tgatcgaagc acgacaagca gttgaaatga
    31501 cgaacttagc ttcgcagatt ttagaccagc tctctggcac ttataataat ttgctgaata
    31561 acaacttgaa tgacacgatg aaatttttga cggtctggtc gctgatcttg accgttccaa
    31621 ctattgtgac aggattcttc ggaatgaatc tccagttacc ctttacccat tccgttttcg
    31681 gatggggaat cgccttgatc atcagtttgg tcttatctat ttggatgctt attgcactgt
    31741 ggcggcggat tcgttgatct agtctttgat ctgcaaacca agggcttggg cgatcacaag
    31801 tgcttgctct tggttgacga ttagtcccgc caaagaatct tgagataaag cgattgctgt
    31861 gaagaaatta gtcgtaaagt ctaatccctt cagagaagtt cgaaaccagt tcgaaccggt
    31921 caactgattg tcagacaaaa aaaggttttt ccaagtgacc tcataaaatt cgctgtcttc
    31981 taattgacaa tgatcgaagg agacagcttt caagtttgta ctactaaagg aagccatcga
    32041 agcaatacaa tcagtgaaag tacagtctcg aagataactt tcggcaaaat tcgtacctgt
    32101 taatttacat tgatgaaaca cgacttggtg gaaactgcca ccaatccact ccagattgga
    32161 aaaatcacat ttttcaaaaa tgacattact gcattcaaaa tgacgtaaaa ctgttttttg
    32221 catcgtaagt ttttggatat gactctctct taataccata tttttggcat cgagatagct
    32281 ttggtcaccc ccgttgatca gaagaccaga gaagatctcc tcatcttcta aataaaactc
    32341 tccctttgta agaagaggaa gagaaggtgg aataggttcg atttttttca tgtgggttca
    32401 cctcgttttt ctttagtata gcaaagaagc gggaatcact gctagaaagt ctgctaatct
    32461 aatcattttc agaaaaggaa gacaaactgt atgaaaaaca ttgacaaaaa gattagtttt
    32521 tgctatacta tgatggttga gaaataaaag cagaagcacc cgcttctcgc cttagttgat
    32581 aatgtcactg ggctagatag ttttcactgg ttctattagg tgaagtacgc gcgggttctg
    32641 taagagaact cgttttttta ttgccgttta ttcggtaagc ttttctttct ctcataatat
    32701 ttggaggtga atgaccatag caaaggatat gatggttaac gacggcattc gtgcacgaga
    32761 gttacgattg atcggttcag atggtgagca attaggagtt aagacaaaag cagaagcatt
    32821 gaatattgca gaacaagcaa acttggatct tgtgttagtt gcccctggtg cgaaaccacc
    32881 agttgcgcga atcatggact acggtaaata ccgtttcgaa caacaaaaga aagaccgcga
    32941 agcgcgtaaa aaacaaaaag tgatcaatgt aaaagaagtt cgcttaagtc ctacgatcga
    33001 cttgaatgac ttcaatacaa aacttcgtaa tgcacgtaag ttcttggaaa agggcgataa
    33061 agtgaaagct tctatccgtt tcaaaggccg tgccattacc cataaagaaa ttggtcaaaa
    33121 agtcttgaat cgcttagctg aagaaactgc ggatattgca acagtggaac aaaaagcgaa
    33181 aatggacgga cgcagcatgt tcttgacgct ggcaccgaaa aacgacagta agtaatttga
    33241 atgactaact ggctgttgga cagccgatag tcggataacg aaagattttg aggaggaaat
    33301 atagtcatgc caaaacaaaa aacacaccgc ggatcagcaa aacgtttcaa acgtactggt
    33361 aaaggcggat tgaaacgctt ccgcgcgttt acaagtcacc gtttccacgg caaaacaaaa
    33421 aaacaacgcc gtcaattgcg taaagcaagc atggtttcta gtggcgactt caaacgtatt
    33481 cgccaacaac tagcaaaaat gaaataagaa gtacaaataa cagaatctga agatttattt
    33541 ttattaggag gaattataca tggcacgtgt taaaggtgga acagtaactc gtaaacgtcg
    33601 taaaagagtg ctaaaattag caaaaggtta ctacggttca aaacacactt tatttaaaac
    33661 agcaaaagaa caagtaatga attcatacaa ctacgcatat cgcgatcgtc gtcaaaagaa
    33721 acgcgacttc cgtaaattgt ggattgctcg tatcaacgca gcagctcgta tgaatggctt
    33781 gagctactca aaattaatgc acggtttgaa agttgctgaa atcgacatca accgtaaaat
    33841 gttagctgac ttggctgtta acgatgcagc agcatttact gcattggctg accaagcaaa
    33901 agaagctttg gctaaataag ctaaacaatt ataaagagga aagaagataa cgaaaagtta
    33961 tgttctttcc tcttttttta tttacaatcc tccatcaaat gaaacaggac aataacgtat
    34021 aagtctaaaa tagcataatt aaggtttttt aattgaaaac tgaacattta tggtcaccct
    34081 ttaatgaaag cgctaactac ataagttaga atgagtttga ctgatatatt agtttgctta
    34141 tcagaaaaac aaaatggacg gaggaataac atgagtgata ttattattgg tattttgtta
    34201 gttatctcat tctttttcat ggtctggtac tgtgtaaaag gctacaactt gatggtcggc
    34261 tttgccatca tggctactgt atggatggga ttagcgctag tcggaaacac gttttcacca
    34321 aacccggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta tacgacgggg
    34381 ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt tttcgggcgt
    34441 gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga acttggaggg
    34501 gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat attcatgtcg
    34561 atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccaat cttgatgtca
    34621 ttaggtattt cagtaccggt tgcactgttc tcatttatgg gctcgattat ggctggaatc
    34681 tttgctaata tcgtcaattt caaacaatat caaacgattt atgctggttt caatccagcg
    34741 gctgaaagct atacttataa tgactatttc caaattggta tgattgggat gattgtaagc
    34801 ttagttgtgg tactaacggt cgctaatata tcaatgaaca aaaagaaacg ctatgcaatg
    34861 gcagcaaacg taccagctga aggtggcgac gcaccaatga tctcatggtt agccgtatta
    34921 ttaccagttt taggggtcgt tcttttagat cttccaatca tcttaggttt tattctagca
    34981 ggtatctggg cattgctgtt tactggaaaa ttgcgcggag gttataaaga aatctgtcgt
    35041 cagtttgcga aattatttac agatggcgca gtagatgttg caccaatggt tggtttcttg
    35101 atgaccttag cgatgttcaa taattcagct gcgtatgctt ctccttattt ttcagctatt
    35161 tttggagatt ggataccgca atcaccatta gtcttggcaa tcgtatttgc tatattgaca
    35221 ccactaggat ttttccgcgg tccaatgaat ctagtcggtt caggctcagc tattttagct
    35281 gtcgtattag ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt atttgcaatc
    35341 acaacgattg ctcctcagca tttagatatt acacaatctt gggttgcttg gggattaggt
    35401 tatacaaaag taacttctcg agaatatatg aagaaatcca tcccaactgg ttggatcatc
    35461 ggagcgatct tatgcctgat cacgttctta ttgtacggga acgcctagta aacaaaacgt
    35521 cgataaaaac gattagatga atggaggaaa agaaaatgaa acgttttgtg cgaatgggaa
    35581 ttgatgttgg cggtacacat acgaaagcag tcgcaatcga taatgcgacg cacgaaatca
    35641 tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg tggagtagcc gcaggtgtcg
    35701 ttcagtcttt tcaaaattgt ttgcgggaaa ataatatcag tccagaagat gttgtgtttg
    35761 ttgcacattc taccacacaa gcgactaatg cactgattga aggagacgta gcaaaagtcg
    35821 gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt agcaaaacga caaactagat
    35881 tgaacgatat cgatttaggg aataagaaaa aaatagagat tgtcaatgca ttcttacctg
    35941 tcaaacactt aaatgtagac cgtgtttcag aaacgatttc ttctttagaa agagaaagag
    36001 cggaggtctt ggtttcttct atggcatttg gagtagataa tggggaacca gaacgagttg
    36061 tttatgaagc agcatcggta aaatcgatcc ctacaacgat ggcttctgac atcacaaaat
    36121 tatatggatt gacaagaaga actcggacag cagcaatcaa tgcatctatt ttaccaaaaa
    36181 tgctagatac ggcaacctct acagaagatt ccgtacgaga agcaggggtc aatgtttctt
    36241 tgatgattat gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa aaacgacctg
    36301 ttttaaccat gctttccggt ccagcagcat cagtcatggg ctctttgatg tacttgagag
    36361 cgtctaatgg tgtttatttt gaagtaggcg gaacaacaac caatatcggg gtcattaaaa
    36421 atggacgtcc agcaatcgat tattcaattg taggtggcca cccaacatat atctcttcat
    36481 tagatgttcg ggttctaggt gtggcaggcg gttcgatggt acgggctaat cagtcaggaa
    36541 tcatcgatgt cggtccacgt tctgcacata ttgccggtct agattatgca gtatttacgg
    36601 agacagaaaa aatcaaaggt cctaaggttg aatttttctc tcctaaagaa ggagaccctg
    36661 ctgattacgt caaggtagtg atggaagacg gagaagaagt gactattaca aatacatgtg
    36721 cagccaatgt ccttggatta gtgcaagaag aacatttttc ttatggaaat gtcccatctg
    36781 cacgaaaagc catccaagca ttagctgatt attgtcatac gacggttgaa gacatcgcag
    36841 aacagatcat ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa ttggcagaca
    36901 agtatcactt ggagaaagat cagatttctc ttgtaggtgt agggggcggc gctgcctcat
    36961 tgattactta ctttagtaat aaaatgggtg tgaaatattc gattcctgaa aacgccgaag
    37021 tgatttcttc tattggtgta gcacttgcaa tggtccgaga tgttgtagaa cgtatcatcc
    37081 cttcgccaag taaagaagat atacgatcgt tgaaaaatga agcgatgaat aaagcgattg
    37141 aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga aatcgatcca cagacttcaa
    37201 aagtaacggc tatcgctact ggttctacgg aagtgaaagc aaccgatcta accaaggaga
    37261 tcacaacaga agaagcacta gagcttgctg cagaggatat gcgattaaat aaaaatgaag
    37321 tttgcttatt ggaaaataca cctttctttt atgtatgcgg cgaacagaat cgttcaaaaa
    37381 atgctggaag tcttcggatc atcgatcaaa aaggatttat caaggtccaa cgtggacatg
    37441 cttcatgtat gaaaacaact gcagccaatt atatgacagc tgtcgaacaa ctttgggaag
    37501 atatggcagt ttatcaaact gaattgattg cccgaccaga attttatctc tgcttaggag
    37561 caagagtctc tgattttaca gcgacggatc tggaacaact acagcttttg atggatctgg
    37621 aagtatctac tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat atcaaacaaa
    37681 cataagttag aagagttact aaacgaatta caacagatcc ccgaagaaat ttggggattc
    37741 taccagtttc ggcgagattt attttggaaa aagattcctt tgtccaagca gaaaattctt
    37801 atacaacaat caatcgattg tggtatcgaa acggcatgta gcatcaaaaa aaagtaccct
    37861 tttgccgatg taggagaaat ttgtgaacaa atggctgttc caatcgtttc ctgtgaatca
    37921 gagcaaataa acgaacgaat cacttttgca acttatgcag aagatgaagg gattcgattg
    37981 atgacggagc cgttagaaaa attaaaatgt tcaggactag cttcgatctc taaagaaaca
    38041 gcgcaagctt tgattattgg acatgaacta tttcaccaca tcgaagcttc tgttaaggga
    38101 atttatacgc aaagcgaaaa aattgtgctg tggcggttgc ctttttatac acatcaatca
    38161 aacattcgtg cattgagcga gatagcagcg atgagttttt caaaagagat gaatcaaagc
    38221 gatttttcgc cttatgtatt agaggcagtc ttactttggc catataatga aattcgtagc
    38281 caagggatct tagaagaaat aaaaaaaata gaaaaaagat gtacggagta tgatttagat
    38341 tacaaataaa gagactgtga caaaagtcgt gtcacagtcc tttttttccg aataaacgat
    38401 gttctcctgt caactcctcg gtcacaagtc aaaatattca acaaacatga gaagctgttt
    38461 tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc ttggaaaacg
    38521 gtgttcaaaa gcttgcatct gcggtaataa gtccagccaa gttaaaaaca taagaagcta
    38581 tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctctcaataa
    38641 acggtgtgca atcagctgac cctcggtatt agctcaaaaa aaacggcaaa atatgaggaa
    38701 ctattttgcc gtttctttct ctaatactca ggtcaaaacg ctgattccaa aacctcttat
    38761 ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt aattatgaat cacgtattct
    38821 tgctcctcga taatcatatg taaatgcatc gtgcaaagaa atccgacttc atcaaagtta
    38881 tgatcaatca ttgattgaag cattgtctga tgttcatcta atactcgacc ccagtctaat
    38941 tctgaaatcg tcaaacgtaa ccatctgaaa cggttcaagt gcgtactata actttctagc
    39001 caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa atcagtatcc
    39061 aaatcaaaaa aagctttttt attttgagcg attactgcct gatcggtttt tccaactttt
    39121 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
    39181 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
    39241 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
    39301 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
    39361 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
    39421 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
    39481 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttgtatc ggtttgacac
    39541 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
    39601 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
    39661 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
    39721 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
    39781 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
    39841 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
    39901 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
    39961 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
    40021 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
    40081 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
    40141 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
    40201 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
    40261 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
    40321 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
    40381 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
    40441 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
    40501 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
    40561 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
    40621 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
    40681 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
    40741 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
    40801 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
    40861 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
    40921 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
    40981 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
    41041 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
    41101 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
    41161 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
    41221 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
    41281 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
    41341 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
    41401 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
    41461 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
    41521 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
    41581 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
    41641 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
    41701 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
    41761 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
    41821 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
    41881 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
    41941 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
    42001 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
    42061 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
    42121 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
    42181 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
    42241 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
    42301 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
    42361 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
    42421 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
    42481 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
    42541 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
    42601 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
    42661 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
    42721 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
    42781 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
    42841 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
    42901 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
    42961 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
    43021 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
    43081 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
    43141 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
    43201 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
    43261 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
    43321 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
    43381 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
    43441 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
    43501 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
    43561 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
    43621 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
    43681 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
    43741 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
    43801 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
    43861 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
    43921 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    43981 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    44041 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    44101 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    44161 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    44221 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    44281 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    44341 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    44401 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    44461 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    44521 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    44581 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    44641 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    44701 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    44761 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    44821 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    44881 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    44941 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    45001 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    45061 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    45121 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    45181 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    45241 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    45301 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    45361 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    45421 ttggataaat caacagtgaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    45481 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    45541 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    45601 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    45661 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    45721 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    45781 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    45841 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    45901 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    45961 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    46021 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    46081 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    46141 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    46201 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    46261 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    46321 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    46381 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    46441 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    46501 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    46561 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    46621 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    46681 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    46741 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    46801 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    46861 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    46921 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    46981 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    47041 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    47101 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    47161 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    47221 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    47281 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    47341 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    47401 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    47461 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    47521 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    47581 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    47641 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    47701 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    47761 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    47821 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    47881 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    47941 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    48001 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    48061 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    48121 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    48181 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    48241 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    48301 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    48361 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    48421 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    48481 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    48541 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    48601 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    48661 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    48721 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    48781 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    48841 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    48901 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    48961 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    49021 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    49081 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    49141 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    49201 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    49261 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    49321 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    49381 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    49441 cgccttatca taagtgatgc ggtttttatc attgatccta aaaatcttag caagacaaaa
    49501 agtacgtaag aaagtaaaga ctaaatactt cggaggaaca aatgaataac aatgataggt
    49561 tattgcgatt gcgttatgcg attgatttga aagatacgga tttgattaaa gcttttgagc
    49621 taggcggtgt accactgact aaagaagaag ctcaggcggt actgaccaaa gtacaggata
    49681 agaacaaaga taatgcagaa aataatgtgt atgaaaaaac catcaacaac caagtttttg
    49741 acgcattcct aaatggcctg attctcttgg cgcgtggtcc gcaaaaagat aagccgatag
    49801 tagacacgcc gcaaaaaagc aaggaaatca aatatatcaa caatgtgttg ctaaaaaaat
    49861 taaaaatcgc cttgtctatg accactgatg atattttaga cgtttttgct gaggcagaaa
    49921 tctatccatc aaaaggcgaa atcggggcat ttttgagaaa agaaggccaa cgaaatttta
    49981 agccttgcgg agataaatat atgcgtaatt ttctcaaggg gttaggaatt tataatcgga
    50041 gaaaagtata aagaaatttc atgagaaatc aaaacaatgg ttgttttgat gagagctagt
    50101 ttttgtatga gattaaaata ttatcttaat cgaggagctg gattggtttt atgaatgatg
    50161 aatataagaa tgatgaagat aagatgcttt ttgaagagat agagaatcga tgtaggctca
    50221 attttgagct tcggggaaag atgtctttaa tacaacaaaa aaagtattta gcgaataaat
    50281 cagagtttac tctgggacat gttgaaaaat taatctcgga ttggattagt agtagaagcg
    50341 aatttacaaa aataaaacag ccgattaaat ttgacatgaa aaaacttttg ttaaataaga
    50401 gtgagatagg taatagagat caatatatta gagctaaagg gcaagaaata attgattcat
    50461 taggtgaaat gaggtcatac aattatttgt atgtaacaca tagagctgat ggaatggtca
    50521 ttactgtggg gaaatcttct tctaatgata tttttttaga tggagatctt ttttatcaac
    50581 tcaatacaaa tcacttgtct ggcactgaaa atata
//
LOCUS       DAYYHX010000002        46922 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000002 DAYYHX010000000
VERSION     DAYYHX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.002"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..1097
                     /locus_tag="ACHA6Y_000051"
     CDS             27..1097
                     /locus_tag="ACHA6Y_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8986180.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            1112..1780
                     /locus_tag="ACHA6Y_000052"
     CDS             1112..1780
                     /locus_tag="ACHA6Y_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8986181.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1800..2378
                     /locus_tag="ACHA6Y_000053"
     CDS             1800..2378
                     /locus_tag="ACHA6Y_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8986182.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2779..3690
                     /locus_tag="ACHA6Y_000054"
     CDS             2779..3690
                     /locus_tag="ACHA6Y_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8986183.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(3778..4695)
                     /gene="rnhC"
                     /locus_tag="ACHA6Y_000055"
     CDS             complement(3778..4695)
                     /gene="rnhC"
                     /locus_tag="ACHA6Y_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM8986184.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            4897..5334
                     /gene="zapA"
                     /locus_tag="ACHA6Y_000056"
     CDS             4897..5334
                     /gene="zapA"
                     /locus_tag="ACHA6Y_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM8986185.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5393..5941
                     /locus_tag="ACHA6Y_000057"
     CDS             5393..5941
                     /locus_tag="ACHA6Y_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM8986186.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            6056..8416
                     /locus_tag="ACHA6Y_000058"
     CDS             6056..8416
                     /locus_tag="ACHA6Y_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8986187.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8503..8817
                     /gene="trxA"
                     /locus_tag="ACHA6Y_000059"
     CDS             8503..8817
                     /gene="trxA"
                     /locus_tag="ACHA6Y_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM8986188.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            8999..10777
                     /gene="uvrC"
                     /locus_tag="ACHA6Y_000060"
     CDS             8999..10777
                     /gene="uvrC"
                     /locus_tag="ACHA6Y_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM8986189.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            10922..11821
                     /locus_tag="ACHA6Y_000061"
     CDS             10922..11821
                     /locus_tag="ACHA6Y_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8986190.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            11814..13076
                     /locus_tag="ACHA6Y_000062"
     CDS             11814..13076
                     /locus_tag="ACHA6Y_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8986191.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13237..15135
                     /locus_tag="ACHA6Y_000063"
     CDS             13237..15135
                     /locus_tag="ACHA6Y_000063"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM8986192.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(15465..16184)
                     /locus_tag="ACHA6Y_000064"
     CDS             complement(15465..16184)
                     /locus_tag="ACHA6Y_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM8986193.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    16374..16421
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            16521..16853
                     /locus_tag="ACHA6Y_000065"
     CDS             16521..16853
                     /locus_tag="ACHA6Y_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986194.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            16840..18843
                     /locus_tag="ACHA6Y_000066"
     CDS             16840..18843
                     /locus_tag="ACHA6Y_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM8986195.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            18862..19332
                     /locus_tag="ACHA6Y_000067"
     CDS             18862..19332
                     /locus_tag="ACHA6Y_000067"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM8986196.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19353..19937
                     /locus_tag="ACHA6Y_000068"
     CDS             19353..19937
                     /locus_tag="ACHA6Y_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFM8986197.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            19952..20938
                     /locus_tag="ACHA6Y_000069"
     CDS             19952..20938
                     /locus_tag="ACHA6Y_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM8986198.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            20928..21239
                     /locus_tag="ACHA6Y_000070"
     CDS             20928..21239
                     /locus_tag="ACHA6Y_000070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM8986199.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21261..23042
                     /locus_tag="ACHA6Y_000071"
     CDS             21261..23042
                     /locus_tag="ACHA6Y_000071"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM8986200.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23035..24411
                     /locus_tag="ACHA6Y_000072"
     CDS             23035..24411
                     /locus_tag="ACHA6Y_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM8986201.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24418..25053
                     /locus_tag="ACHA6Y_000073"
     CDS             24418..25053
                     /locus_tag="ACHA6Y_000073"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM8986202.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25083..26438
                     /locus_tag="ACHA6Y_000074"
     CDS             25083..26438
                     /locus_tag="ACHA6Y_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8986203.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(26630..28024)
                     /locus_tag="ACHA6Y_000075"
     CDS             complement(26630..28024)
                     /locus_tag="ACHA6Y_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8986204.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            28116..29087
                     /locus_tag="ACHA6Y_000076"
     CDS             28116..29087
                     /locus_tag="ACHA6Y_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8986205.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            29084..29866
                     /locus_tag="ACHA6Y_000077"
     CDS             29084..29866
                     /locus_tag="ACHA6Y_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM8986206.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(30035..30406)
                     /locus_tag="ACHA6Y_000078"
     CDS             complement(30035..30406)
                     /locus_tag="ACHA6Y_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8986207.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            30540..30983
                     /locus_tag="ACHA6Y_000079"
     CDS             30540..30983
                     /locus_tag="ACHA6Y_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8986208.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(31026..31835)
                     /gene="yidA"
                     /locus_tag="ACHA6Y_000080"
     CDS             complement(31026..31835)
                     /gene="yidA"
                     /locus_tag="ACHA6Y_000080"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM8986209.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(31854..33221)
                     /locus_tag="ACHA6Y_000081"
     CDS             complement(31854..33221)
                     /locus_tag="ACHA6Y_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8986210.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(33205..34035)
                     /locus_tag="ACHA6Y_000082"
     CDS             complement(33205..34035)
                     /locus_tag="ACHA6Y_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM8986211.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            34198..34635
                     /locus_tag="ACHA6Y_000083"
     CDS             34198..34635
                     /locus_tag="ACHA6Y_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM8986212.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            34711..35331
                     /gene="rpoE"
                     /locus_tag="ACHA6Y_000084"
     CDS             34711..35331
                     /gene="rpoE"
                     /locus_tag="ACHA6Y_000084"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM8986213.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            35520..36026
                     /locus_tag="ACHA6Y_000085"
     CDS             35520..36026
                     /locus_tag="ACHA6Y_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8986214.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            36043..36942
                     /locus_tag="ACHA6Y_000086"
     CDS             36043..36942
                     /locus_tag="ACHA6Y_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM8986215.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            37029..37913
                     /locus_tag="ACHA6Y_000087"
     CDS             37029..37913
                     /locus_tag="ACHA6Y_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8986216.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(38105..38176)
                     /locus_tag="ACHA6Y_000088"
     tRNA            complement(38105..38176)
                     /locus_tag="ACHA6Y_000088"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38141..38143),aa:Arg,seq:ccg)
     gene            38236..39570
                     /gene="rpoN"
                     /locus_tag="ACHA6Y_000089"
     CDS             38236..39570
                     /gene="rpoN"
                     /locus_tag="ACHA6Y_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM8986217.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            39719..40810
                     /locus_tag="ACHA6Y_000090"
     CDS             39719..40810
                     /locus_tag="ACHA6Y_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM8986218.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            40851..41852
                     /gene="gap"
                     /locus_tag="ACHA6Y_000091"
     CDS             40851..41852
                     /gene="gap"
                     /locus_tag="ACHA6Y_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8986219.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            41962..43152
                     /gene="pgk"
                     /locus_tag="ACHA6Y_000092"
     CDS             41962..43152
                     /gene="pgk"
                     /locus_tag="ACHA6Y_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM8986220.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            43256..44011
                     /gene="tpiA"
                     /locus_tag="ACHA6Y_000093"
     CDS             43256..44011
                     /gene="tpiA"
                     /locus_tag="ACHA6Y_000093"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM8986221.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            44127..45425
                     /gene="eno"
                     /locus_tag="ACHA6Y_000094"
     CDS             44127..45425
                     /gene="eno"
                     /locus_tag="ACHA6Y_000094"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM8986222.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            45824..>46922
                     /locus_tag="ACHA6Y_000095"
     CDS             45824..>46922
                     /locus_tag="ACHA6Y_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM8986223.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDM"
BASE COUNT        15577 a         8133 c        10022 g        13190 t
ORIGIN      
        1 atgattattt caggaggagg agttaaatgt ttttagcatt aaatgaaatt atgcattcaa
       61 agttacgtta tgcgttagtt gcaggggtga tgtttttgat cgcctacctg gtattttttt
      121 tgactgggtt agcttatggc ttagctcaag ataatcgaac agcagtcgac aagtgggagg
      181 cagattcgat tgtgctttcg aaagatgcaa attcaaatct agggatgtcc atgattacta
      241 agaagatagc agaagaagtc gaaggtggga aagtggcgta tcttgcccaa acacctggag
      301 tcgtgactag taaagacagt acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg
      361 ataaaaatca attcatcatg cccaatcttg ttgaaggaaa agcttttgat aacgatgatg
      421 aggcagtagg cgatattagt ttgaaagaag aatatggtct agccatagga gatacagtta
      481 agttatctgg tagtgacaag acgtttaaac taactggctt tacggatcat gccaaattca
      541 atgtctcacc agttctctat acaaccatca atgcttacca agaaatccgc ttcgaaaaag
      601 aagatacaag tgaaaatgca cggattaacg caatagttgt cagaggtaaa atcagtaatt
      661 tacctgaaga tttagaacag atcaaaattt ctaaattcat caatgaatta cctggatata
      721 atgcgcaagt attgactttt ggatttatga tcggattttt gatcgttatt gcggctattg
      781 tgatcgggat tttcatttat gtactgacga tgcaaaagat taatatcttt ggtgtaatga
      841 aagcacaagg gatcacagga ggatttattg cgcgttctgt agtggcacaa acattcattt
      901 tatcatttgt tggtattctt cttggtctat taggaacagt gggaacatcc cttgttctac
      961 ctgatgcggt tccattccaa agcaattggc tgttttttgg cgtcattagc ttgttgatgt
     1021 tagtagtagc tgtgttaggc gcactatttt ctgttcggac aatcgtaaaa atcgatccgt
     1081 tgaaggcaat tggataagaa ggaggaagaa catgaaagca atagaattta aagaagtaga
     1141 aaaaagtttc caagatggcg accaagtagt taaagcattg aaagaaacaa ccatgagtat
     1201 cgataaaggg gaatttgttg caatcatcgg tccttctggt tcaggtaaaa gtactttttt
     1261 gactttggca ggcggattgc agacgcctag cagtggctct gtatttatca atgatcaaga
     1321 attcagtaat caaaaagaaa gcgaacgcgt taaactacgt tttaaagaaa ttggctttat
     1381 tttgcaagct tccaatctgg ttccttttct gacagtaaaa aagcagttat tattagtaga
     1441 taagattaaa catgaacatc gtcaaaaaga agcaaacgaa ttatttgagc agcttggcgt
     1501 ggataagttg atcaacaagt atccagaaga actatctggt ggagaacggc aaagggtcgc
     1561 aatcgctcgt gcattgtaca atgatccgtc cattattcta gcagatgaac caactgctag
     1621 tttggattca gaaaaggcac gagaagtagt cgatatcttg gcaaaagaat caaaagacaa
     1681 aaataaagca acgatcatgg ttactcacga tacacggctg attgacaaat gcgatagaat
     1741 atttatgatg gaagatggtg tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa
     1801 tggtccatcg atacaacaaa gatacacagc aaaaaatttt gaaagaagcg actgacttgt
     1861 ttatgtccaa gggatatctc ggcacttcaa caagagaaat cgcacaaaaa gttgggatca
     1921 cccagcccaa tctctatcat tatttcggtg ataaagaaaa actatatacg gcagttttag
     1981 aaaaccactt aaaagatgtt ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg
     2041 gatttcaagt aaccttgaca aaaatggcgc agtttttgat tgagacacat ctggtcgatt
     2101 tgtttctgat gcttcacgat cttgaaagca atctttcaaa ggaaacaaga gatcatcttt
     2161 tctttctatg gaagaaaaat tatcgtgaac cttttgaaga aatcttttca gcaaaccaat
     2221 ctgtgctaag aaatgggatt tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag
     2281 caccttacat cactcggtcg aaaagacctg tggaagaacc gctaaatgtg gcgcaattga
     2341 tcgatttata tttgaatggt gtattaggag aagcgtaaag ctgttatata gggagaaaat
     2401 agttaggtga agaagcaaga tatgacgaag atcgttatat cttgcttctt tatataggta
     2461 gtttttcata aagacaaatt tgttattctc tcatgattat cagggtatat ttttatttat
     2521 tatgtcggtg ttccaggaag tgttttaatt taataacgaa gaaattaatt gtttgtagaa
     2581 tgaaggaaac tatttattgg gtagatgaat caccacgaat agtcaaaagg agatttcagg
     2641 atatgccaaa aatgaaaaaa atcattttag cagtaatgtt attttctacg gttatatctt
     2701 ttacagcccc ttttttgctt acttaagctg attttaagtt ttggccctta gtctggatct
     2761 acgaggtgag agtcaagaat gaaaaaaata attggagctt ttgtatttgc aatcattatc
     2821 attattatct gttttcaaat agatgttttt ggtcaaaaaa agatagttgg acaaacggtc
     2881 agcagtacga acagtacggt tacaaagaca aaggaatcat ttgatgatac agaacaagag
     2941 acattaatca aaatcggaaa aaatgaccgt acactatata cagatgactc gctaactacc
     3001 ccacttgcaa ctataaacgg cggagagctg gcggagtttc tttctgaaac aaaaaatgct
     3061 tatcagatca agacaaatga tggctatact ggatatttag ccctcgaaga tggaaccaaa
     3121 gtcacaaaga atatacaaac aaaaccggaa gagctatctg atgcagtgat tgtattagat
     3181 cctgggcatg gcggaaatga tactggggca ttaagcaatg atgaacaaac agaagaaaaa
     3241 gatataacat taagtacagc cataaaagta aaaaaggcgc tagaggatgc tggagcaacg
     3301 gtttatttga cccatactac agatgaatta gtacagcttg gagatatatg cgactacagt
     3361 gaagaaaaaa aggcagatgt ctttatcagt ttacacgcag actctacgga atatgcgaat
     3421 gaagcaactg gaatcacgac atattattac tatggacaag aggaaacatt agcacagaca
     3481 attgctgact ccttcactga tctgcctctg gattcaagag gaatctccac tgggaactat
     3541 caagttcttc gagaaaatct tcaaccttct attttaatcg agatgggtta tatgaataat
     3601 gatagtgatt taaaggaatt agtaacagac agttatcagc agcagattgc tgcaagtttg
     3661 acacaagcat tgaccactta ttttcagtaa taaataagcc cagccaaaca aaaatagctc
     3721 ctcatatttt tcgtttggcc gggcttatta tcgcagatgc aagcttttga acaccgttta
     3781 tttttttagt agcttcaaag ctttttctgt atttgcaaag tgaagtttgg catatttttc
     3841 aagcataggc atgccgcctt tctccaatag ttttgccgct acttggtcag atttcgttcc
     3901 agctcctgat ggaagggtga ttccagcttc agctgatgct ttgtgtaact cttccaaaaa
     3961 gctggcacga ctgatgatag aagcagcagc aacagctaaa tgatattgtt ctcctttagt
     4021 aacgaagtac agtttttctt gtaattgatt tttttctaaa cgcgcatatt ttttaaaatt
     4081 tgcttccggc gtaaactgat cgatcaaaat cgcttctggt ttttctggct cgattttttt
     4141 tagcagcaaa tggatcgctt gattgtgcaa agcgactttc atccgaacgg cattgtattt
     4201 tggttggatc tcattgtatt tttctggaga taagacaagc agtttatagg ggatggtttt
     4261 ttttaagatg tcagcaagct ggatgatttg gctatctttt aactcttttg aatctctaac
     4321 accaagttta cgtaattctg caagctgatc acgtttcaca taagcagcac atactgttag
     4381 cggaccgaaa tagctgccat ttccgacctc atcactacct aaaacggata attgactgat
     4441 attttttggc aaagaagagg ctgtttttgc tggcttggct tgtttatcct gtgcctcccc
     4501 ccatttatca gcttctattg ctgcattcgt accttgaaac atcacttttc cggatgtata
     4561 agcggtaatg gtcgtcccgt ctttttttgc agcaaagcta gtataaggaa tctgtttatt
     4621 taacaaataa gagcgatagt atgctttcat ttgactaagt tggtcgtttg ttgctttgat
     4681 aatgacgttg ctcataaaat tctcctcgtt tttatacata aaactatacc gcaactcacg
     4741 acttttcgtt ttctaagaga ttgcgagaca tagcattttt ttaaagttta tcatataata
     4801 gttaagtatg tgaaaaatat ttgtggagaa cggtgaaaga accaacttca tggtacacta
     4861 aataaaactt tcatcaaata ggaggaaatg attcgtatgg cgcacgaaaa aacacgctac
     4921 aaagcggtga ttgccaacca aacatatact attatcggac gggaaaccaa gcatcatatg
     4981 gatatcgtaa cgaagctgat caatgaacag ttagcagaat tgaaacaatt atcaccacaa
     5041 atggataatg aacaagcagc aatcttgatg gctgtcaatg cgctttcgga tcaattgaag
     5101 aaacaagaaa gaatcttgga gctggaagaa gaaacagcag aattaaagaa aaaaatgatc
     5161 aaatttacag agcttgaaaa tcgtgtgaaa cgcattgaag caatcgagaa cgaagctcgg
     5221 gaagtattga aagaaaatgg tcaagcagat cataccatta aaaatcatgt ggaagcacaa
     5281 cagattttaa acgagaaacg aaaaggacaa attaaacaaa aatcttctaa ttaagatcct
     5341 tttatcaaaa taagaaaaat ttaaaaaaag ttagaagaga aaggaacgta gcatgctgag
     5401 tttgctgatt ttatttttat tgctaattgc gtttttttct ggagcacgtc gcgggtttgc
     5461 tttgcaaatc atattcgcga ttggatatgt attgtcattt attgctgccc agcattttta
     5521 caaaccatta gcaagtcatt tggaattata cattccatac ccagctgtca cgccaacatc
     5581 aaagttagca ttttttgatc aggtatttgc ctttcaccta gatgaagctt tttatgcagg
     5641 aactgctttt ctgattattt tgctaattgg ctggctgctt acccgatttg tgggggtttt
     5701 tgtccatgga ttgacgtatg tgccgatctt gagacaggta gactggattg caggaggcat
     5761 cctgtcacta atcatggctt acgttactat atttttgatt ttgcagttac ttgcctttgt
     5821 tccattagat gtggtccaaa atcaattcgg cgagcatagt ttcgctcgct ttatcgttga
     5881 gagaacgcca tttcttacca acaagatgta tgagctttgg gtaacgaata ttttgaatta
     5941 aggaaaatag tgctggaaaa tatttcgttt tttgtacaga gtagattatt taagaaggct
     6001 gcgtagcgta tgtccggcac agctcttttt tcatagtgaa agataaggtg ataaaatgaa
     6061 taaccgaatc cttgaaacat tggaatttga aaaagtcaaa cagatggtca gacaatttgt
     6121 agtgactgca caaggaaaag aagaactggc agaattggta ccagtgagcg aggaacaaac
     6181 gatcacaaac tggcttcaag aaactgagga tggattgaaa gtgcaacgtc tgcgaggcgg
     6241 gatccctata cctaaacttg aaaatatccg tccccatatg aaacggattg agatcggagc
     6301 agacttgaat ggagtggagc ttgcacaagt ttccagagta ttgtctacga caagcgaatt
     6361 gaatcgtttc attgatgatc tttatgacag tgaaatcgaa tttgcacgtt tgtatatgtg
     6421 ggcagatcag ttagtcacga tccctgtttt gagccgccgt ttaaaggaag caatcgatga
     6481 agacggacgc gtgactgatg acgcttctcc tgagttaaag agcataagac agaacattcg
     6541 gcgaagtgaa caagctgttc gtgaacagtt agatgggatc gtccgtggga aaaatgcaaa
     6601 gtatttaagt gatgcgatca tcaccatgcg aaatgaccgc tacgttatcc cagtcaaaca
     6661 agaataccgc ggtgtatttg gcggtgtagt ccacgatcaa agtgcttcag gacagacttt
     6721 atttattgaa ccaaaacaag tggtggattt gaataatcgt ttgcgtcaat accaaatcgc
     6781 tgaacgaaat gagattcaac gtattttaag tgaactttct gctgaacttg ttccccatcg
     6841 tcaagaaatc atccacaatg cttatgtgat tggtaaaatg gatctgatga atgcaaaagc
     6901 acgatttggt aaagaagtaa aagctatcgt gccgggaatc agtgaagaca atcatgtcgt
     6961 cttgaaacaa gcaagacatc cactgatcga tcaggaaaaa gtagtttcta atgacatcac
     7021 aatcggaaaa gactatcaag ccatcgtcat tacaggacct aacacaggtg ggaaaacgat
     7081 cacgttgaag actttaggtc tgttacaact gatggggcaa gcaggattac cgattcctgc
     7141 aggtgaagaa agtcaaatcg gtatttttga ggaagtattt gcggatatag gagacgaaca
     7201 gtcgattgag caaagtctat cgaccttttc ttctcatatg acgaacactg tagatatttt
     7261 atcaaaagtc aatgaaaaaa gtctggtatt atttgacgag ctcggtgcag gaacagatcc
     7321 gcaagaaggt gctgctctag caatcgcaat cttagatgat cttggtaaaa agtctgctta
     7381 tgtgatggca acaacgcatt atcctgaatt gaaagtttat ggatataatc gggcaaatac
     7441 gattaatgcc agcatggagt ttgatgtgga tacattaagt ccaacgtatc gtttgctgat
     7501 tggcgttcct ggacgaagca atgcgtttga aatctctagc cgattaggtc ttgatacaga
     7561 agttattgat gaagcgaagc agttgatgaa tgatgagagc caagatttaa acgaaatgat
     7621 cacagattta gagaatcgcc ggaaaatggc agagaccgaa tatctggaaa tgcgtcattt
     7681 tgtttctgag gcacaagaac ttcacgatga tttgaaagaa gcttacagct atttctttga
     7741 agaacgtgaa aaagaaatgg aaaaagcaaa gaaaaaagca aatgaagtcg tgtcagaggc
     7801 ggaagaaaaa gcagaaaaaa tcattgctga tattcgtaaa atgcaacagc agattgggca
     7861 aggaaatgtg aaagagcatc agttgatcga tgcaaagacc caattagcaa atctacatca
     7921 agaagaaact ttgaagaaaa ataaagtatt gaagaaggcg aaagaacaaa aaacgttgaa
     7981 accgggagac gaagtgttgg ttacgactta cggccaaaga gggacgcttt tacgtaaaaa
     8041 cgggaatcag tggcaagtag aaatcggtat attaaaaatg aatgtgtccg aagacgagct
     8101 gacgcctgtt gctcctcaaa aagagccgac acaacgagtt atccatgctg tacgatctga
     8161 atctagtagt cacgtgccga atcaattgga ccttcgagga aaacgatacg aagaagcgct
     8221 aagtgaggta gatcaatatt tggattctgc cattctagct ggttatcctc aagtgactat
     8281 tgtccacgga aaaggaacag gcgcacttcg caagggaatt actgattatc ttaaaaacca
     8341 tcggagcgtc aaaagctttg agtttgcacc ggcaaaccaa ggcggaaatg gtgcgacaat
     8401 tgttaaattt aagtaaagct tactaaaggt aagcagatgg ttcgctatcc aagtatagaa
     8461 atgctatact agtcatagat tttaaaaatg gaggtctttg atatgtcaca agtaattaca
     8521 gataagacat ttaatgaaga aacagataaa ggacttgtat tgattgattt ttgggcaact
     8581 tggtgtggtc catgtcgtat gcaagcaccg attttggatc agttggaaca agaatatgat
     8641 gaagaagaat tcagaattgc taaaatggat gtggatgaaa atccagaaac tcctcaacaa
     8701 ttcggtatca tgagcattcc aacattaatg ttgaaaaaag atggtcaagt agtagaaaaa
     8761 gctgttggtg ttcattcaaa agaacaacta cgtcaaatga tcgatcaata tttataaaaa
     8821 cttacagcaa aaataagctg agggtgggac tgaatccagt tccgtcctcg gtttttttgc
     8881 ggttgagtta aggttattca aattaatatt ttttgagtat aattacgttg tcaggctgga
     8941 ttgttttttt gacagacatg ctataataac tttaattgaa tatgaagggg agtaccagat
     9001 gaacgaacga atcaaaaaca aattagcttt acttcctgat cagcccggct gttacttgat
     9061 gaaagataaa aatggaacga tcatttatgt cggcaaagcc aaaatattaa aaaatcgtgt
     9121 ccgctcatat tttactggta gccataatac gaaaacggaa cggcttgtta gtgaaatcga
     9181 agactttgaa tacatcgtta cagaatcgaa tatcgaagca ttgctcttag agatcaatct
     9241 gataaagaaa aatgatccta aatacaacat tatgttaaag gatgataaaa cctatccttt
     9301 ccttaagata acgaatgaaa aatatccccg tttggtgatt actcgtaagg tactaaaaga
     9361 caaagcactg tattttggac catatcccga tgtcggtgcg gcgaatgaga caaagaagat
     9421 cctcgatcga ttatttcctc tgagaaaatg caaacctagt cagacgaagg agccttgtct
     9481 ttactatcat ttgggacaat gtctgtgtcc ttattatttc gacgtagatc cgaaagtcta
     9541 tacgaaaatc gttgatgaag tgaaaaactt tttaaacgga ggacacgaaa aaatcgagtc
     9601 agagatccat cagaaaatgg aaaaagctgc tgaaaacatg gagtttgaaa aagctgcaga
     9661 ataccgagac cagatccgtg ccattgagac aatcatgact cgccaaaaaa tgacaaatac
     9721 tgatttactg gatcgagatg tatttggtta tgcgattgat aaaggatgga tgtgtgtcca
     9781 agtgtttttc gtaagacaag gaaagctaat tgaacgagat gtttcaatgt ttcctttcta
     9841 taacgaagca gaagaagatt tcttgactta cctcggtcag ttttatcagg aaaatgagca
     9901 tttcattcct aaagaagtct tgattcctga tacgatcgac aagcccagtg tagaagcttt
     9961 gttagctacg aaagtgctgc aaccaaaacg aggagaaaag aaaaaattag ttgaattggc
    10021 gagtaagaat gcctcagttg ctttgaatga acggtttgat ctgattgcca gaaaacagga
    10081 acggacgatc ggagcagttg aacgtttagg aaacgcgatg aatatcccga ccccaatacg
    10141 tatcgagtct tttgataact ctaatatcat ggggacgaat cctgtggctg cgatggtcgt
    10201 attcattgat ggaaaaccgg caagaaaaga atatcggaaa tataaaataa aaacagtcaa
    10261 cggaccggat gattatgcat caatgagaga agtcatctat cgcagatatt cgcgagtgat
    10321 tcgcgaaggg ttgcctttac ctgatttgat cttgatcgat ggaggaaaag gacaagtaga
    10381 tgtggcaaaa gatgtcttag aaaatcaatt aggaatcgat attcctattg ctggcatggc
    10441 aaaaaacgat aaacataaaa caaatgagtt gctattcggt acagatttgg ctgtagtacc
    10501 gcttgagcga aattcaccag agtttttctt actccagcga atccaagatg aagtccatcg
    10561 attcgcaatc actttccatc ggcagctacg cagtaaaaac agttttgctt cccgattaga
    10621 tgaaattgaa gggttaggac caaaacgaaa aaaaatgctg cttaaagagt tcaaatcgtt
    10681 gaaaaatatc acagcagcca cgacagaaga attacaagaa atcggactgc cgaaaaatgt
    10741 cgcacaaaac gtatttgata aattacatca ggaatgagga aagggcactt tacaaagaaa
    10801 gtaaagtgtc ctatcatttt atgtacaaca aaaagagcgt ttacgttttg ttggtaaata
    10861 ataaatcgct tacgtctggt gtctaaggat atatgtgatt tcttgaagaa ggagtgaaga
    10921 aatgttagaa gtcaaggatc ttgttaaaac ttttgggaac tacacagctg tcgatcatgt
    10981 ctctttccaa atccccgatg gaaaaatcat ggggctaatt ggccaaaacg gggctgggaa
    11041 gacaacgact tttcgtttga tcttggattt tttgacacaa gaccaaggag aagtgttatg
    11101 gaatggccat catcttgggg agaaagatta tgatgtcatc ggttatcttc cggaggaacg
    11161 gggactttat ccgaaagtaa cgattcaaga ccaactgctt tattttgcag aacttagagg
    11221 gaaatcaaga aaagagattg aaccaaaaat cgacttttgg atggaaaaat ttcaggtaaa
    11281 agggaaaaag acggataaag taaaatcttt gtctaaagga aatcagcaaa aagtccagct
    11341 gatcgccacg ttaatccatg aacctaaatt gatcattttg gatgagccat tcagcgggtt
    11401 agatccggta aatgctgaat tgctaaaaga tggaatattg gaattgaaaa aacatggttc
    11461 atgtgtcatt ttttctagcc acaatatgga taatgtagaa aaaatctgcg atcatttgat
    11521 catgttgcga aacggaaaaa tggttttgaa tggaaaagta catgagattc gtgaatcatt
    11581 tggccgtacg aagttattct tggaatctgg tttaagccaa caggaagtag aagaaatcga
    11641 aggcgtgaaa aaagtgatcc ttcatgaaga caatacgtta gagattacct tagaagatcc
    11701 tgaagctggg aaagaaatct ttactcgtgc gactcagttc ggctacattc ctatgtttaa
    11761 ccagcaaccg ccaactttgg aagagatttt caaattgaaa gcaggtgaaa tcgatgcgta
    11821 agttttggat tattaccaaa gatgtctatt taaaaaatgt taagtcgatt tctttcttga
    11881 tcatgatatt agtccctttt atcatgctgg gtatcattta tgtagcagga aactttgctc
    11941 aacaaaatag tgatgtcaat aaaatcggcg tattggctga taatgagcaa atcagtcagc
    12001 aaatgagcca agtcaaaaca gatgattttg agttcaaagc agttgattct gaagaaaaag
    12061 cacaaaaaga gctttcggaa gaaaaaatcg atgcgtattt agtagtctca actgagaatg
    12121 ataagataaa tgggatgctt tatagcgaaa attcattagg acaaagtaca caactactga
    12181 tacaacaaca actgactggc ttgcaatcga tgcttcgagc gagtcaatta ggcatttcac
    12241 cagaagaagt agctagcttg agccaacaag caagtttgac tcgacaaaaa gtcaattttg
    12301 attcaaaagg aaaaatgaca gtcggagaag ataactcgac agtccaatac gtcgtaagtt
    12361 atgtagcaac gattgtcttg ttcattatta ttttgacgta tgcgcagatc attgcacaag
    12421 agatcgcttc tgaaaaaggc acgcggatca tggaagtgat cctgtccagt acgactgcac
    12481 aaaagcattt ttacggaaaa ttaacaggtg ttttacttgt tgcattaacg caaatggcat
    12541 tatacggtat tatttttggt ataggataca gtcaattcaa agatatggaa atggtcaaaa
    12601 atatattaga cggtatctca ttggataact tgattggacc attcttgtgg tattctcttc
    12661 tattcatgat ctttggcatt ttgatttttt ctgttttagc agctttatgt ggctctttgg
    12721 tcaacaaagc agaagataca gcaaaagcaa ttttgccagt gacttatcta tctctaggcg
    12781 gatacatgct tggattgata ctaggagctt cagatccaaa caatatcgtt attcggatca
    12841 cttcgtatat tccattctta tcttcatata ttatgccggt tcgcttagca aatgagacag
    12901 tcggtatggg tggagcattg atttccttgt tggtacttgt agccgtaaca ttcttattga
    12961 tgatcttgtc tgcaaacatg tacaaatcga atgtactcgt ttatagcgaa ggcggattat
    13021 ggagttcatt gaaacaatcg atttcaatta tgaggaatga acgaaaaaaa gcataaaaca
    13081 atcctaaaat acagaaggaa gctggacatc tattgttccc agcttctttt tgtatagacc
    13141 tattttattt attttactat attggatata tattcattga aaatagtgta taacaacttt
    13201 ataatattga gtataccaat ttttggaggc gttttaatgg accgttattt agatttactt
    13261 tcagaaaaat tcccctcaac ggaggatgtc atcacggaaa tcatcaatct cgaagcaatt
    13321 ttgcagcttc caaaaggaac agagttattt ttgagtgata tacatggcga atttcctgct
    13381 tttgatcata ttctgcgtat cggttcagga aatcttaaag aaaagatcaa agaccttttt
    13441 tctgaacaaa tgacggaaac ggatatcagc caattcacta tttttacggc ttatccagaa
    13501 tatgcattaa cgactgattg gtacaaagaa caagataaat cgcagctgat ccatcatttg
    13561 atcgatcttt tacggtttac gacagtcaaa tatacccggt caaaagttcg aaaaggattg
    13621 ccgaaagaat atagctatat cattgaggaa ctcttgtata tcgatgaccg tatcaatgga
    13681 aaaaaagact atgtaaaaaa aatcattgaa caacttgttt tgatgaagga agaagaaaaa
    13741 tttttaacga aacttgcaca gacgatccaa aaatctgtca tcgatcattt gcatatcgtt
    13801 ggagatattt ttgaccgagg aactcaagca gccaaagtga tggatcgaat catggatttt
    13861 tcttctgtag atatccaatg gggaaatcat gatattttgt ggattggtgc gtattgcggc
    13921 tctgaggcat gtttactgac acttttacgg attgcagcac gctataatta tttatttgaa
    13981 ttagagaaag aatatgggtt gaatttacgt ccattgtttt cttttgctca tcaaacttat
    14041 cagaagaatc cagtatttgc gcctaaaaac agtaaaaatc tatccgaaag agagcaagaa
    14101 gaattagaaa aaatccatca ggcactttct atccttcaat tcaaatcaga acatcagtta
    14161 ttggatcgtc gacctgaatt taaaatggac gatcgtaaat tgctggaaaa aatcgactat
    14221 gaagcatcaa cgattgattt agaaggtcag ctctatgagt tgaaaggaac ttgttttcaa
    14281 acgatccagc cagatgatcc aaaaaaattc ttggaagagg aacaaaaagt gattgatagt
    14341 ttgatgtatt cattccagca ctctttgaga atgcaaaaac atatgacgtt tttgatcgaa
    14401 aaagggggaa tgtatctcac gaacaatcag catgtgctgt ttcatggctg tatacctgta
    14461 gacgagaagg gacagctctt agcatttgaa ttagaccaat cttatcgcgg aaaagaactg
    14521 ctgggctttt ttgaaaaaca gataactgaa agcgcaaaag atctgactgc aaaagaagat
    14581 ttagcaaccg atttgatttg gtatgcgtgg agtgggccat tttcgccttt attcggcaag
    14641 agtaaaatgg cgacttttga acgttatttt ctcacagagc ctcatacgca cgtcgagaaa
    14701 agcaatcctt attatcactt gcgagatgaa gagtggttca gtgaaaaaat tttagcagaa
    14761 ttcgacacaa aagaagaagg gtcagcaatc attaatgggc acacgccagt aaaagtaaaa
    14821 aaaggggagt caccgatcaa agcaaatgga aaagtatttg tcattgacgg aggtttatcc
    14881 aaagcttatc aaaaaacgac aggaattgcc ggttattcac tattatgcaa ttcttatggt
    14941 ttccaaatcg tgacacatta tccgtttttg cctattgaac aacaatttgc aaaaaaatcc
    15001 gaccaaacaa atgtgaaaaa agtaatcgaa cagcaactac cacgcaaact caatcgtgat
    15061 acaacaaaag gtatggaact gcagcagcag attacccagc tcaaaaggct gttggactat
    15121 cgaaaggacg attgagaaga attccagtga acattgttcc atagactgga gactgagaca
    15181 aaagtcgtgt cgcagtctct ttttccggat aaacggtgtt cttctgccaa ctcctcggtc
    15241 acaagtcaca ctattcaaca aacatgaaaa gctgtttttt gaaattgctc cttgtgactc
    15301 agagttgaac ggcaggctca cgacctcttg gtataaacgg tgttcaaaag ccagtttctc
    15361 ggtattaagc caaatttatg caaaaatgtg aaaagccatt ttcacaaaat ttttcttaat
    15421 actcgaaact aaacagcttt ttcacaaccc cctcagaaag tctattaaaa ttcattcaca
    15481 aagaaatcga ataagcgctg ttcagatgga tgtacacggt gggtaagttc cggatgccat
    15541 tgaacaccga ggattttatg catatcatct ttgctttgaa ccgcttcaat gagtccatcg
    15601 ttagaaaaag cgatggcttt taataaaggt gaaagatctt tgactgcctg atgatggtaa
    15661 gaattcacaa aatatggatc ggggagaagg ttgcttaaca aactgtctga aacaatcttg
    15721 acttcgtgag tcgtaaattg cggggcagtg ggctgttgtt catgttttac tgaccacttt
    15781 ggatataatg aaagatcttg ataaagtgta ccttccaaaa cgacattcaa tagttggagt
    15841 ccccggcaga ctgcaaaaat tggttttctt tgtttcaatg cttccaagat cagtgcctgt
    15901 tcaaattggt ctcgttgaat atttgtttcc gttagctttg gatgaggatc ttccatgtaa
    15961 agttgtggac tgacatcttg accacctgca agaataagtt tgtcgatttg tccaatcagt
    16021 tgaggagctg aatctggaga catgactggc agaataagag gcaatccttg agcatctaaa
    16081 actgcatcca cgaatccttg cggtgtatag ctcacttgat ttccttggaa agtatctgtt
    16141 gctcgaatca gttgatttcc ggcaatgccg atgattggtt tcatttgtat tccccctaaa
    16201 gtagacttat tgtaacaagg atttaaaaaa aattaaacta tgtaagactt gcccctatac
    16261 ttatctgttc ttgctgtgtt actatctgta aagagaaatt tcgtgtgaaa ttcatgacga
    16321 tagttgaaaa tattgcttaa tttgttataa taaaaagtaa gcagaaataa aaaggttggg
    16381 cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg acattttatt gaaggggcct
    16441 gcctatacta agttatcttt agaggtacat actttcaatg aaaaaagaaa gcatgtacct
    16501 cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa atccgaaaag
    16561 cagaagaaga aaatgaaaag cgtgctcaaa cactgcggca gcagctgaag atctatgcaa
    16621 cagaaaaaga gcaggaattg gaaacgatcc aaacaaacta tcgacaacaa ctaaaacagt
    16681 tggtggaaga aaaagaacaa tttgaaatac aagaattaga aaaagaaaaa atgaaattga
    16741 aagcagaagc agacaaggtg caagcaactt tgaaagatca atatcaaaag catcagagtc
    16801 aagcgatcgg tgcgatcatt gaaagggtga aagaaactta tggccgtcac taagatggaa
    16861 aaagtgaccc tcatttcaga taaaagaaat caggagagga ttttacaagc agtacaagga
    16921 atccaaaacg ttgaaatccg ggatctgtat caaaatagaa caaacaacca gtgggtggag
    16981 aagtattttc cagataccac gattattgat aaagaagcaa aattaagcgc tttaggtaat
    17041 cggttgaaag acatccaaga agctatccaa ttcatacaac accacggcga tagccgtcaa
    17101 aagaaactcc atttgaaacg tcaagaactt tccttacaag atttggaagc caattattcc
    17161 gaagaaaatt tcataaataa attgaaagaa gtccttgatt taaaagaaaa gtgggaacaa
    17221 ctaactgaag aacgtgatcg tttagaagaa gaggaagaat ggcttcttca ttggcaacag
    17281 ctggatattg ctccaagtat gtatgcaagt aagacgacca tttttgagat ggggactgtg
    17341 agtgcagcaa attttgaaag tttcaaggaa gaactgagta agcttgatga cgtttatatg
    17401 gaagaaatag attatacgcc aaaacaagtt tatctttcgt atgttgcttt aaaaaaatca
    17461 gttgctgtta tagaagaaat tgctgggaaa tacgggttca ataaagaaac gtatccttat
    17521 gaacaaaatc ctaaagaaaa actgcaagag accaagcagc aattatccat tgttcacgag
    17581 cagcaaaaga agataacgac agcgttaggt cagtgcagtg gctatatcca agaatttgaa
    17641 tgggtagaag aagtcacact tgccgttgca gaacgagaaa aaataaaaga acggtttatc
    17701 catgcgactt atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa acagaatttg
    17761 ctgcatgtgt taaacgaaac cgtatcagaa gaagatatat acgtcagttt cgaaacacca
    17821 aactcagaag agattcagca ggaagtccct actaaattga aaaaccatcc gcttgtcgaa
    17881 ccttttgaat tgctgactga aatgtacagt ctgccaaaat atgaagaagt agatccaaca
    17941 ccttggatga caccatttta tcttgtcttc ttcgggatga tggtcgcaga tgttggttat
    18001 ggtttgctga tgctcattgg gacaatacta gctcaaaaac tacttgttct accacgcggg
    18061 atgaaacgtt tcgtaaaatt ctttgagatt ctctcgatac catcgatcat ctggggattg
    18121 atctacagtt catttttcgg acagtcatta ccaaaagaac tgtttggcat ccgcttgcct
    18181 ttcccaatcc tatctacgac ggatgacgtc aatacgattt tgattttatc cgtgattttc
    18241 ggtctgatac agattttggt tggattattt gtggcagcaa aggaaaatat caggagaaaa
    18301 gattacttga gtgctgtcag tgatggattt gcctggcaag ggatcctaat tgggatcgtt
    18361 attgcgttgg ttggtgccat gttgataaaa aatacagcat ttgtttacct tggcgctggg
    18421 ctatcgatca ttgctgcctt atgtatcttg atcgttccga ttatccaaac gccgtctaaa
    18481 gtcaaaggtg ctgccaaagg tgcgtataat ctttacggac tgaccggcta tatcggtgat
    18541 ttagtcagtt atacacgttt gatggcattg ggtatttcag gcggaagtat tggagcggca
    18601 ttcaatatgc tagttgcttt catgccgcca gctgctcgat tcagtgtagg gatccttttg
    18661 atcatcgctt tacacgcact gaatatgttt ttgacactgc taagtgccta tgtccatggc
    18721 gcacgattgc agtatgtcga attttttggg aagttttata caggaggcgg acgtgccttt
    18781 gatccgctga aaacggcgga aaaatacgtc aacatcaatc acaagaaaaa aaacaataaa
    18841 taattggagg atttttatat aatgatggat tacttaatta gtcaaaatgg tggaatggta
    18901 tttgcggtat tagcgatggc aacagcgacg attttttcag ggatcggttc agctaaaggt
    18961 gttggaatga caggtgaggc agcagcagca ttgacaacaa gtcaacccga aaaattcggg
    19021 caggctctga ttctgcaatt gcttccaggt acgcaaggtt tgtatggatt cgttattgcc
    19081 tttttgattt ttatcaactt aggaaatgac atgtcagttg tacaaggact gaactttcta
    19141 ggtgcttctc tgccgattgc ttttacaggc ttgttttcag gtattgccca aggaaaagtc
    19201 gcagcggcag gtattcaaat tttagcgaaa aaacctgaac atgctacaaa aggaatcatt
    19261 tttgctgcga tggttgaaac atacgcgatt ttaggtttcg ttatttcctt cctattagtg
    19321 ttgaatgcct aagcataaag gaggaaaagc agatgaacgc cattgaaaaa atcatctctc
    19381 agatgaacga agcagcagag caggaaagag cggcgttaga acaagaagag cgaatgaaga
    19441 tcgatcaaaa ctttgaacaa aaacgtacgc aagttgaaac agaacatcaa aaacaaaaag
    19501 aaaaacaaat cgaattactc gaaaaaaaat accgtcagct aaggaatcgc cagcaagtag
    19561 aagtacgaca agaaaatttg aatgcgaagc aggaattttt aagacgattg tttgcagatg
    19621 cggtaactga aatggaaaat tgggatgaat ctgaacaaat ccagtttatc aaaaatgcat
    19681 tgtattcatt acctttgaca ggaaaagtag cattcattgc tggagaaaaa tcagcagcct
    19741 atctttctca gacgttgttg gatgaatgga acaatgaact tccctttatg atggttttaa
    19801 gtgatgaaac tgttgctgat caagcgggtt ttctgataaa cgatcaagga gttcagtata
    19861 attttctttt cagcagcctt gtacaagata tccaaggaac gatgagtttt gagattgcca
    19921 atcaattatt cgaataagga gggatcagtg tatggaatac cacgaactaa atcccttgat
    19981 tcgcggaaga gagcttgaac ttttgtccaa tgagacattt gaacggatga ttcaaacaaa
    20041 ttctgtcgaa gcacttggtg aaatattgaa atccacgatt tacagtccat atatttatga
    20101 cggatatgaa tatgacttcg aacagaatct tttaaaggag cagagccgtc tatttcgctg
    20161 gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc tatacgatgc gttatacttt
    20221 ccataatctg aaggttttga caaaagctga gatgacaaat caaaatctgg accatttgta
    20281 tatcgacgat ggattttatt cattagaaac cttgaaggat gccattcata cgcaagtttc
    20341 aaatgaactt cctgaaagca ttatggacag tatacgagaa gtacgtgatt attttgaaga
    20401 gtctaccatt cttcagggaa ttgacgtgat ttatgatcga cagtttttga cagagcagcg
    20461 acggcttgcc gataagctag gatatcaaga attgctgaca gaagtcattg cttttattga
    20521 cttgaccaat atcacaacga tggctcgagg aatattgcag aaaagaacat atgggttcat
    20581 gacgacggta ctttccagct cgggaagtat cgaaaaagaa gtatttttga actttgtaga
    20641 taaagatatg gaagattaca cgaattttct attagagaca gattatgctg attttctgcg
    20701 ccctgtcatc cgtgagggaa atattgatct cgtaaagctt gaacagttga aagatgatta
    20761 tctaagttca ttgtatcaag aagcacaaac ccaggctttc ggaccactgc cactactagc
    20821 ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta tcggcaaaag
    20881 aagccatttc gaatcagaaa caatcagaaa gcgggtgaga caagtctatg acgcataaga
    20941 tcggtgtagt tggcgacaaa gattctgttt tgccattcaa attgtttggc tttgatgttc
    21001 gttatggtac aaccaagcaa accgttcgtc aggcaattga agaaatggca aagaatgagt
    21061 atggcgtgat ttatgtcaca gagcaatgtg cagcattgag cccagaaacg atcgaacgtt
    21121 acaaggaaca aatgatacct gctatcgtat tgattcccaa tcatcaagga tcattaggga
    21181 tagggctgga agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat atcttatagt
    21241 atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc ggacctcttg
    21301 taatggctga gaacatgtct gatgccagta tccaagacat gtgtctagtg ggagatctag
    21361 gagttatcgg agaaattatc gagatgcgtg gcgatgtggc gtctattcaa gtatatgaag
    21421 agacttctgg aattggtcca ggcgaaccgg ttcgctctac tggtgaagct ttatctgttg
    21481 aattaggacc agggatcatt tcacaaatgt ttgacggaat ccaacggcca ttagatacct
    21541 ttatggaaat cacacaaagt aatttcttag ggcgcggagt tcaattgccc gcattggacc
    21601 atgagaaaaa atggtggttc gaaccaacag tagaagccgg agaaacggtg agtgctggag
    21661 atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg gtgccaaatg
    21721 ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt cactattgat gaaacagtat
    21781 gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa tggccagtaa
    21841 gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc acccatgatc actggacagc
    21901 gagtgattga tacattcttc ccagtgacaa aaggaggagc agcagctgta cctggtcctt
    21961 ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa atgggccgat gtggacatgg
    22021 ttgtctatgt gggatgtggc gagcgtggaa atgagatgac ggatgtttta aatgaattcc
    22081 ctgaattgat tgaccctaat acaggagaat cgttgatgga acgtacggta ttgattgcta
    22141 atacatcaaa catgccagta gcagctcgag aagcttctat ctatacggga atcacaattg
    22201 cggaatattt ccgtgacatg ggttatgacg ttgctatcat ggcagactcc acttcacgat
    22261 gggcagaagc acttcgtgag atgagcggac gtttggaaga aatgcctggt gatgaaggtt
    22321 atccagctta tcttggttca cggcttgcgg aatactatga acgatctgga agagtcattg
    22381 cattaggatc agaaggtcgc gaaggaagta taaccgcaat cagtgctgta tccccgtctg
    22441 gaggggatat ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa gtattctggg
    22501 gtcttgattc cagtttagcc caaaaaagac attttccttc gatcgattgg atacaaagct
    22561 attctcttta ttcaacagaa gtagggaagt atatggatca tatccttcaa gctgactggg
    22621 catccatggt caccgagggc atgcgtattt tgcaggaaga acagcagctg aatgaaatcg
    22681 tgcgtttggt agggattgat tccctttcag ataatgaccg tctgacatta gaagtggcaa
    22741 aatccattcg tgaagattat ttgcagcaaa atgcttttga tgatgtcgat acattcactt
    22801 caagagagaa acaattcaaa atgttgaaga tgatcttgac ctttggtgaa gaaggacgcc
    22861 gtgcgttgtc gttaggtgct tatttaagtg agatcatgga cggtaccgta tcgattcgtg
    22921 aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt agaaaaacta gatcaaatta
    22981 ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga aggaggcatg agcaatgatt
    23041 aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga tggcggttga aaaagttgct
    23101 ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat tcgtcgtgga
    23161 caagtattgg aagtccaaga agataaagca atggtccaaa tattcgaagg aacaagtggt
    23221 atcaatttac gtgactcttc tgtacgattt ttaggccatc ctttagaact aggtgtatcg
    23281 gaagacatga tcggtcgagt attcgatggt ttgggacgac cgaaagataa cggaccagag
    23341 atcctgcctg aaaagcattt ggatattaac ggagaagtaa tcaatccagt agctcgggat
    23401 tatccagatg agtttatcca aacaggtatc tctgcaatcg atcatttgaa tacattagtt
    23461 cgtgggcaaa aattaccagt attttctggc tctggtcttc ctcataaaga attagctgct
    23521 cagatcgcgc gacaagcaac ggtactcgat tcatcagatg atttcgccgt agtctttgct
    23581 gctattggaa tcacttttga agaagctgaa tttttcatgg aagattttcg tcaaacgggc
    23641 gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg accctgcaat cgaacgtatc
    23701 gctactcctc gtatggcttt gactgctgca gaatatcttg cttatgaaaa gggaatgcat
    23761 gttttggtta tcatgacaga catgaccaac tatgcagagg cattgcgaga gatttctgca
    23821 gcacggcgag aagttccagg aagaagagga tatcctgggt atctttacac gaacttagct
    23881 actttattcg aacgagcagg aagaatccgc ggactaaaag gatcggttac tcaaattccg
    23941 attctaacaa tgccagaaga cgataaaact catccaatcc ctgacttgac tggttatatt
    24001 acagaaggac agatcatctt gactagagag ctttacaaga gtgggattca gccaccaatt
    24061 gatgtactcc cgtctctttc gcggttaaaa gataagggaa ctggacccgg aaagacaaga
    24121 gaagaccatg cagctacgat gaatcagctt tttgcagcct atgcacaagg aaaacaggca
    24181 aaagaattag ccgtagtttt aggtgaatct gctttatctg atgtagacaa gatctatgcg
    24241 aagtttgctg agcggtttga aaaagaatat gtcaatcaag gattctacac gaaccgttcg
    24301 attactgaaa cacttgatct agggtgggaa ctgctatcga tgttgccaag aaccgaatta
    24361 aaacgaatca aagacgacat gctggacaaa tatctgccag aagggaagtg agttctgatg
    24421 gcacgattga atgtgaatcc gacccgcatg gaattgactc gattgaaaaa acagttgaca
    24481 actgccacaa gagggcataa attattaaaa gataagcaag atgaactgat gcgtcaattc
    24541 atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc tatgacagaa
    24601 gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg aacctttcat cgaagaatta
    24661 tttgttttgc cggcggaaaa cgttgatttg tctatcgtag acaaaaatat catgagtgta
    24721 aaagtacctg tgatgaattt tgaatatgat gaacagctga cagacacacc tttggaatac
    24781 gggtatttga attccagtgc cgaactggat cggtcaatcg atcggtttac taccctgctt
    24841 ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc aactgatggc gagtgaaatc
    24901 gaaaagacac gtcggcgagt caatgcatta gaatatatga cgatccctca attagaagaa
    24961 acgatctatt atattcgtat gaagctggaa gaaaacgagc gcgcagaagt aacaaggttg
    25021 atcaaagtaa aaaatatggg taccactgac taagtggtat ctttttttaa aagaggaaaa
    25081 cgatgattaa acaaagcaaa caaataaaga aacgtttgcc ggctgttcag cttctcgcat
    25141 taggattttt tctcctgatt ttagttggcg gcggcctact taccctacca ttttttagta
    25201 atagtgggca tgggacaaat tttatcgatg cgcttttcac tgcaacttct gctgtctgtg
    25261 taacgggatt gacaacattg aatacagcag agcattggaa cagtgctggt caatttttga
    25321 tcatgctttt gattgagatc ggcggtcttg gttttatgat ggttccgatt ctatgctttg
    25381 cattgataaa gaagaaagtc agtctcagca cacgtattat attaaaagaa gcgctgaatc
    25441 tagaagaagt atcaggtgtg atgaagctca tgctttacat cctaaaattg gcctttgtga
    25501 tccaattgat tggtacggtt gctttatctt ttgtatttgt gcctgaattt ggctggttga
    25561 aaggtatctg gtacagtttg ttccatgcaa tctctagttt ttgtaatgca gggtttgatt
    25621 tgttaggaga cagtttagcc aatcaccagc aaaatgttta tttgatcatg gtggtatctg
    25681 ctttgatcat ttctggtggg ttaggcttca tcgtatggcg tgatttactg gaatatcatc
    25741 agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac cgttacaggt acccttttat
    25801 taggcggatt cgttgtgttc tttttcacag agcgcaatgg ggcaacgcta gtggaaggga
    25861 atatgtttca acgattggca aacacctttt ttatgagtgt gacacctaga acggctggat
    25921 attattcagt tgattacctc catatgagcc atgcaggtct gattttaacg atgtttttga
    25981 tgtacatcgg cggaacatct gggtcgacgg ccggcggatt gaaaacaact acgttaggtg
    26041 tgttgctgat ccagatgcgt tcgatgttga aaggacgtac gagagcggaa gcatttggcc
    26101 gtaccatccg acaaggcgca gtgttgcggg cacttacttt attttttgtt acgttgacgt
    26161 tgtgtgtagt cgccatcatg attttatcgg tgactgaaac gatacctaaa acttctggca
    26221 tcgaatatat tgcttttgag gtcttctctg cgtttggtac agtaggtctt acgatgggat
    26281 tgacgccaga cttgactgca atagggaaat tgatcattat agcgcttatg tacattggac
    26341 gagttggtgt attgactgtg gtcttctctc tgatgacaaa agcacaaaga acacaatcaa
    26401 aatacaaata tccagaagaa agtgtcatga ttggataatt tcatcttctg acagaaacct
    26461 agtgatttag taaaataaga aaataaaata atttttcttg cttcttggat actttaagtg
    26521 caaaatgttc gtttattttt tgaggcacta cccgttgttg aacctcgggt agtgcctctt
    26581 ttttattgaa aggaatcaat aagatttgat tcctttattt atcaataggt tataattcat
    26641 atttttttat tttttctttt acgatactta gtccagaagc taaaatttca aggttattag
    26701 aggataggga aattcgaacg aatgcatctg gttgcgtacg tgtagtaaag cgtctggaat
    26761 gataaagtcg gataccgtta gcaagaaagt cattctcaat tatcttaggg tcaatctcag
    26821 gatcaactgg aagaattcga tagtaaggga cacgattcgt tggtttttct actttgaaaa
    26881 gagaatcaaa aagctgattg gctctttttg taagacttaa tttagtttct aagatcctat
    26941 ttgcttcatt tgaatggagg atttgagtga tgacttccac atctaatcca gaagttttca
    27001 catttatatt aaaaattgct tgttctatct tgctttggac atgtactgga actacaagat
    27061 aagcaactcg caatccagaa caaataaatt ttgtcattcc agctaagtag attgtttggt
    27121 taggtaataa attttggaaa ggaggaatga tcgtatgttg atagtaagtt gttaaaaatg
    27181 aatggatatc atcttcaata acccatagac cttctttttc aatcacttcg gctaattgct
    27241 ctcgtctttt gtgagagatt tgaaacccta taggattatt acaagaaggc ataagaaaga
    27301 caccatgaat cttctttttt ctacattcct gaatcaatat atcaggtcgc attccttgct
    27361 cgtcaaaatc aattggaacg atctctaaat gatataactg agccaattca atgaaattag
    27421 cgtatgtata tcggtctgtt gctattctat cacctggaga gaaaagtgca gaaagtgtga
    27481 tagccaatcc attttgaaca ccggaaacaa tagatagaga ttctaaggac ggaatgactc
    27541 cttgagtttt taaccaattt gctgcagtag tcaattgaaa atttgtgcct ttgggatctt
    27601 ggtaattaag caaaccaaca agatttgggt tattactagt tgattgaata aacgggacaa
    27661 cgaatttatt acattcttca aaagaactaa ccagaccaaa atcaatgata tgttcggtta
    27721 cttcattggt agagatggtg atggactcaa agacattggg agaaataaat gtgccacttc
    27781 caatattcgt atacagcaaa cctttctcaa ttccatattt ataagccttg ccaacggtag
    27841 taaaatttaa atctaaataa tcagctagct ctctttggga gggtaactga gtgtttttct
    27901 gtaatttatt ctttaaaata tcttgttcta attgttgaat caacgatcga taataaggac
    27961 gcttcaattt ttgtttgtct ggtcgccaag ttaacgggta attgtcataa gaattaatag
    28021 gcattttctc cactccttaa aattgtatac catacaattt taccattgat tgtattgatt
    28081 ttagaagtta atcttttgtg aggagggaat tgtctatgaa aattattaat tttgaggaag
    28141 tagtagcttc ttttagtttt gaagaagcga ttcaagtaat gaaaaaatgt ttttttgatt
    28201 atgaaagtgg taaaatcagc caaaatcctc gattggtgga aattttacct gacggaaaag
    28261 gcaaaaatat gtttgcgatg atgcctgctt atttggggga gaacagatat tttggcacaa
    28321 aagtcattac cgcttttccg gataatcatc agtatcattt accttctcat ttaggagaga
    28381 ttttcttgtt tgatgctcaa aacggactac ccaaagcatt gataaacgca aatgcagtca
    28441 cctggattcg aacagctgct gtatctgcgc tggcaacaga ttacttagca aatccgcatg
    28501 cagaaacact tgctttaatt ggagctggtc aacaagcatt gtcgcatttg actgcgattt
    28561 ctaccattcg tcccatcact acagtgtttg tttacgatat tttagaagaa agacgaaata
    28621 cttttatcgc taaagctaaa aaacagtatc ctaaaataac gtttataaat gcaatatcag
    28681 tagaagatgc agtacgtcca gcggaaatca tttgtacgtt gacctctagt aaagaggcat
    28741 ttcttcaaaa agagtgggta agggaagacg cacatatcaa tgctgtgggc acatttactc
    28801 ctgatacaag agagataagt acatcgttga tggaaacttc tcaaatctat gtagatgatt
    28861 actcagctgc cttaacagaa agcggggacc tgcttatagc catggcagaa ggagatttag
    28921 aggaatcttg tattgttggt acattaggcg aattagtttc tgagaaaaaa acattttata
    28981 aaggagaatc gaggccgacg attttcgacg cagttggtct agctattgaa gatctttgtt
    29041 gtgcagaata tatttataac aaattagaga aaggggcttt aaaatgaact tagattatta
    29101 cgtagttgat gcatttgcag atgaggtgtt caaaggaaat ccagcagcgg tttatgtgct
    29161 agaagaatgg ctgccagaag gtacgatgca aaaaattgcc attgaaaaca atttatctga
    29221 aacagcattt acagtgaaga aaaaccaaga atttgagctt agatggttta cgccagatcg
    29281 agaaatcgat ctttgtggtc acgcgacatt agcaacagca tttgttttat ttaactatta
    29341 caaaatacct gatgaaacga ttaaattttc tactcaaagc ggtaatctat ttgtgacaag
    29401 aattaaagat tattattaca tggattttcc tagtattatg ccgaaaaaag tgccgatatt
    29461 gtctgaatat gaagaagcga ttggggctaa gattaaagaa gcctacttag cacgtgattt
    29521 atttttcgta ttagaagatg aaaaaacggt ggctaaattg cagccagatt ttacagctat
    29581 caaaaatttt gaattaggaa taggagtcat cgtgacagca gaaagtgtgc aaaaagactt
    29641 tgtttctaga accttctttc caaaattaac aatcaatgaa gatccagtat gtggatcagc
    29701 acattctaat cttattcctt attgggcaga aagaatgaat aaaacaaaat tgtcagctta
    29761 tcaaatgtca tcaaggggcg gggatctaga atgtgaatta aaagaagatc gtgtgataat
    29821 aggaggaaaa gcagttttgt tttctaaagg tacctcgttt atttgatttc tttcaaatga
    29881 attaaaaata aattggtgga gagtggcaac cttatagcta caatggttgt taaaaaaaga
    29941 cgcaaacttt ttcaatttaa tgaaaagttt gcgtctcatt ttaattattg gtgtttgttt
    30001 tttattcaat caagagtaaa atgccccata ctaactatcc tcggatgact tcgattttat
    30061 aaccatcagg gtcaatgaca aagtagtaag aaggcgcagt gccaggtaat ccttttaagt
    30121 cagtaatctc aagaccagca gctttatgtt gctcgtgtaa tttttctaga tcatcagctg
    30181 ctatagcgat atgaccatag ccgtctccta gatcatacgc accatgatca tagttgtaag
    30241 tcaactctaa ttcatagtca tccccaggaa gtgtcaaata aacaagtgta aatttgtttt
    30301 ctggaaaatc acggcggcgg ctttcttcaa aaccaaatgc cttttgataa aattcgattg
    30361 atgcttcaag atctttcact cgaacacatg tgtgagccat tttcatagat tcatctctcc
    30421 ttttcctttt actcaatcat aacaaaaaga agtaaggaaa tagttgattt gtgcttgttc
    30481 atgagaaatt tctcatgtaa aatggataga aaataaaact gttaaggtgg aaactattca
    30541 tggataaacc aatttttggt gaaaaaaagc aatttgctga ttatcaaacc cgttatgcag
    30601 catatattgt catagaaaga gatcagcaga ttgcagtaat cgaggctcca aatggtgctt
    30661 tttttcttcc tggcggggaa attgaaggag aagagacgaa agaagaagcg attgtccgtg
    30721 agctgataga agaaatggga attgtggcag aaatttcttc ttatctggga gaagctgatg
    30781 agtattttta ctcgaattat cgtgcaactt attattataa tccaggatat ttctttgttg
    30841 ctgattcttg gaagcgtata ggagaaccaa ctgaaaagac caataaaatt tggtgggtga
    30901 gccctcaaga agcgcttgtg aaattgaaac gtggtagcca ccgatgggca gtcgagagat
    30961 ggatcgaaaa aaataaaaac tgaaagaaag cgagtagatc agctttctct cagttttttt
    31021 atttattaca gtacatattt tttgattgct tcagctacac cgtgctcgtc gtttgatgaa
    31081 gtgatgacat ttgctgcttc tttgacaaga gaaacggcat ttcccattgc tactcctaga
    31141 ccagcatatt caatcatgga taagtcgttc tcgttatctc cgatagccat gatttcatct
    31201 tgatcaatac caaggtgctg tgcgagattc gcaactgccg cacctttact tgcttcttta
    31261 tttagtactt cgaagtaata gggcgcactt ttgaccgttg tgtatttatc acggaaagtt
    31321 tgcggtaaac gcgcaattgc tgcatctaaa atttctggtt cgtcgatcat catcattttg
    31381 acgatgttca tgtctggtgt catttcttca ggtgttcgat attttagcgg catcttgact
    31441 aaacttgctt cgtggatcgt ataagcgctg atatctcgat taggcgtgta gatcgctttg
    31501 tcatcgatcg aatggagatg actgccgaca cggcgtgcca tcacttcgat ctctaggaaa
    31561 tcttcatgtg tcagtccgtg acgggcgatg attttacctg atttcgtttg ttgtacgagt
    31621 gccccgttat aagtaataac gtaatcatta tcttgaaaaa ggttcaactc gtttaactgt
    31681 tcctggacac ctggtaaggg acggccagtg cataaaacga tcgaaacacc ttttgcaaca
    31741 gcagcggcaa tcgcttcttt tacttctttt gttacttctc ttttctcatt taataaagta
    31801 ccatcgatgt cgatagcgac tagtttgata gacataattt cctccaatat attttaattt
    31861 atttcaatca atgcgccatt acggatatgg ctggcaaatt cttgataagt ttcgtcaaat
    31921 aaatcataat gatcacgcaa ggacggatca accatttctt ttggaaagaa aaatcgctca
    31981 tctccttgtt cttgtcccgc taatgcagca acaagctgac taacctgtga taattctatc
    32041 aaagttccgt catttcgcaa aatctcgatt tgtgtgcgat gtctgccttg ttctgggcga
    32101 taaaaatcat aaggtagatc gtagctagaa ttcacagcag tgtaataaac aggattatat
    32161 ccgacctttt ctactaacaa agtgagttct tggactaatt ttgaatcccg gttgccagaa
    32221 aaagtagcag atttcagtgg tttacgattc aaaaaacgtt tagctaaatc atttaaaata
    32281 ggatcagcag attcagacca ttgagtaaag taagtgccca agaccccatc atcaagtttg
    32341 aggtattctt ctaaagtaaa gcttccgtca agaaatggca gaagtaattg agaatggagt
    32401 tcaaaactgc tggcatcttc ttgatacaac tcgtgtgcgc gatgaagcag atgctccaaa
    32461 attacttcca taccacggga aacaggatgg aagtacactt gcacatacat ttgatatcgg
    32521 ctaacgatat aatcttccac tgcatgcatg ccactcatgg aaaaagcgat cccgccttca
    32581 taaggacgaa tcacacgtaa tattcgggta agatcaaatg ttccatattc tgttccagtg
    32641 aaataagcat cccgaagcaa atagtccatt cgatctgcat cgatctgact agaaatcatt
    32701 tggacaactt gtgggttggg gtaagttttt tggatcacac tagctacctt ttcaggaaaa
    32761 ccttcttcta ctcggttaag aatctgatac acttctgttt ctggagaagt gatgatctct
    32821 acagtgatgg cttcatgatt cgtatggaag atatgttcaa atgtatgaga gtaaggacca
    32881 tgaccgacat catgcaacag tgccgcacat aaagtcacca gccgttcctg atcatcccag
    32941 cctccgttgc ctattttttc ttttgaaaaa tttcgagaaa agatatcgca gattcgtctt
    33001 gaaatctcat aaacgcctaa tgaatgagta aaacggctat gttctgctcc gtgaaaagtg
    33061 aaagaagaag tgcctaattg tttaatgcgt cgtagacgtt ggacttcttt cgaattgatc
    33121 aaatctaaga ttacttgatg ctggacatgg atatagttat ggactggatc acgaaaaact
    33181 ttttccatag gcagcatttg atatttataa ggggttgtca taatgtagct cctttatgtt
    33241 attacgttgt tccattctct ggatttggtt gttccattgc tccagctgga tttctttttt
    33301 tagccagtcg gtagaatcct gtaaatcact tgcagaatac agcttttcaa aagcttttgc
    33361 aaatgcttct ttgacttctt ttacaaaaag gggttggtca agcagttctt caagtgttgc
    33421 cattgtagca ggatccactg caggatagcc atttgtacca aagtcttctt ttaaagcaga
    33481 ttgataaaat cgcttcatga cttgcccgcg tttttcctga ttaccgttga cacttaagta
    33541 catcataaca gaaatcccat ttttgatccg acgctgagcg ataccggcaa attttttgcc
    33601 atggatgctt aaatcataag ttccaggaca ataagaatgg gtgatttcga atgcttgtat
    33661 cggtgccttt ggaaaagcgt atttagtcaa taggagcatt tgctcgtaag cgtcatctat
    33721 ggttattttt tttgtgtcac tttgggggaa aatcaaagaa atattcaaaa ttccttcatc
    33781 tgatacgatc cctaatccac ctgaatttct gatcactgga gcgtaacctg catctttcag
    33841 tgtttggatc ccgtcactta gaaaaggagt tcgaacatcc ttcataccta agatgactgt
    33901 gcagtcatat tgccagaaat gtaaaaagat ttgctggtgt tctttagaat attccgttaa
    33961 tatatctgtt aatgcaaaag gggaaaagtc gtttttttga taaatgtgct gatctagtcg
    34021 aatcgcaggg ttcattcaac cggctccttt gtataaattc atattttcta caatattata
    34081 ctctaaaatc aaagagttgt tttggaaaaa aggttttctg taaaaatgga caagttggtt
    34141 gacaaaacaa aagaaactag gaacaatgta gctatgagaa gtaaaggaga atgaataatg
    34201 gaattatcac aaggaacacc gatttcaatc aaattgcgta caaaagtaaa acagcaaggg
    34261 gaagtgcaag atttttattt tgatttgaaa gggcaaatgg tgacaattgg cgatacgctg
    34321 tacatacgtt ataaagagat tcaggaggag acgggcgatg agattcctgt aacgatcaaa
    34381 ttagttccag atggtcatgt acagctgatc cgtgcaggag aaatgcggat gcgtttgaaa
    34441 tttggctata aagaacggtt agaaacatcc tatcgaacac catacggtat gatccagatt
    34501 gctactttta cgaaagaact gcatgttagt ttgaaagacc gtcccacagc agggaaggtt
    34561 cgaatcgatt atgatctgtt catgggaccg gaaaaaatcg gcgaatatta tttgaccttg
    34621 gattttacag cgtaaatata aaagaaaagt ttgatttttt gtgtctgatt ttgtatgata
    34681 aggagtagac tgtgaaagga cgtgtcctga ttggaaatta gtgtatttga aggtgcaaac
    34741 aaaagcgaat tatcaatgat cgaagtagca cacgcgatct tagaacaacg tggcgatgtc
    34801 atggattttt ctgatttagc taaccagatc caaaactatc ttgggaaatc agacagtgaa
    34861 attcgtgatt cactcgcaca attttatact gacttgaata ttgacggcag ctttatctca
    34921 ttaggggata atcgttgggg cttgcgttca tggtatgcta tcgattcgat cgatgaagaa
    34981 gtaaaccatg gaatggacga agaggatgaa gatactccac gtcgccgtaa acgcaagaaa
    35041 gtcaacgcct ttatcaatga tgatgaagac gcgatcgatt acaatgacga tgacccagaa
    35101 gatacagatc tgacagaaga agatgacgat gatctgtttg atgacgatga tgatgaagat
    35161 gaagaaatcg ctgcttataa ttctgacttg caagaaatcg gagcagatga cccgacagat
    35221 gatgaagaag acttgccagg aaatatcgaa gaagatttaa gtatcataga cgaagacgat
    35281 gatgatgaag attatgaaga ggaagaattt tctgactctg aaaaagaata aacattattt
    35341 tcttacgaga ggttgtgaca gagacgatca gcctatcaga ataaggaggt gtgacacgat
    35401 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
    35461 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
    35521 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
    35581 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
    35641 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
    35701 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
    35761 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
    35821 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
    35881 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
    35941 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
    36001 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
    36061 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
    36121 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
    36181 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
    36241 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
    36301 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
    36361 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
    36421 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
    36481 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
    36541 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
    36601 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
    36661 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
    36721 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
    36781 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
    36841 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
    36901 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
    36961 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
    37021 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
    37081 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
    37141 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
    37201 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
    37261 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
    37321 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
    37381 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
    37441 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
    37501 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
    37561 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
    37621 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
    37681 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
    37741 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
    37801 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
    37861 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
    37921 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
    37981 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
    38041 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
    38101 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
    38161 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
    38221 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
    38281 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
    38341 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
    38401 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
    38461 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
    38521 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
    38581 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
    38641 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
    38701 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
    38761 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
    38821 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
    38881 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
    38941 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
    39001 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
    39061 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
    39121 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
    39181 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
    39241 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
    39301 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
    39361 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
    39421 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
    39481 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
    39541 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
    39601 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
    39661 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
    39721 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
    39781 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
    39841 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
    39901 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
    39961 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
    40021 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
    40081 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
    40141 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
    40201 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
    40261 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
    40321 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
    40381 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
    40441 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
    40501 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
    40561 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
    40621 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
    40681 catggcaatc gcgggcggaa aatcaaaagc taaagcaatt cgagcttata tgaaaaatgc
    40741 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
    40801 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
    40861 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
    40921 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
    40981 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
    41041 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
    41101 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
    41161 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
    41221 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
    41281 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
    41341 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
    41401 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
    41461 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
    41521 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
    41581 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
    41641 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
    41701 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
    41761 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
    41821 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
    41881 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
    41941 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
    42001 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
    42061 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
    42121 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
    42181 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
    42241 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
    42301 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
    42361 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
    42421 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
    42481 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
    42541 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
    42601 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
    42661 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
    42721 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
    42781 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
    42841 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
    42901 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
    42961 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
    43021 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
    43081 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
    43141 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgaaca aaaggtgaag
    43201 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
    43261 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
    43321 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
    43381 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
    43441 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
    43501 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
    43561 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
    43621 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
    43681 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacagg tgtcaaacat
    43741 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
    43801 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
    43861 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
    43921 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
    43981 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
    44041 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
    44101 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
    44161 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
    44221 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
    44281 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
    44341 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
    44401 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
    44461 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
    44521 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
    44581 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
    44641 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
    44701 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
    44761 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
    44821 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
    44881 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
    44941 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
    45001 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
    45061 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
    45121 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
    45181 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
    45241 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
    45301 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
    45361 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    45421 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    45481 cttagttaag taatcgaact ctctacttgc tgaaatatca gcaggtagag agtttttttg
    45541 caactagctt ctgtatatga tgtttacaaa gaatggtaca agaaaagatc atccttgaaa
    45601 aaggaaagct aacaataact agcatcatat aagagctttt aaggatagaa cagtagaata
    45661 ggaatctatg agcaagtcct ctcgtataaa taaaatagga gggaagtagc agcatatttt
    45721 tcataaagtg gctctctttc ttcattttta ctttggttgc tcctatttga aaattcgagc
    45781 acactcaaat acaataaaaa gaaatggcaa gggagaggat tagatgaaaa aagaagaaaa
    45841 aatcgcgatt ttacaagaga tcatccgaat taaatcagtg aatggaaacg aaggtgaagt
    45901 agcagcctat ttgaataagc ttttggcaaa gcatgacata acaggagaga ttgtttctta
    45961 cagagatggt agagataatt tgattgctcg ttaccaaaag ggccaatcag gaaaagtgtt
    46021 aggcctatct gggcatatgg atgtcgtggc ggctggtgat gaatcgtctt ggacctatgc
    46081 accctttgca gcagaaatcc atgggaaccg tttgtacgga cgtggtgcta cagatatgaa
    46141 gagtggcttg gcagcaatgg tgattgccat gatcgaactg aaagagtcag gaaaaccatt
    46201 taacggaaca gtcaaattat tagccactgt cggagaagaa gtgggtgaac taggtgggga
    46261 acagctcacg aaagcaggct atgtagatga cttagatgcc ttgatcatcg gagagccaac
    46321 caattacagt ttgatgtata cgcacatggg atcaatcaac tatacagtca cctcgcacgg
    46381 aaaagaagcg catagctcta tgccagacca aggttataat gcaatcaatc acctaaatga
    46441 attcattacg aaagcaaatg ctgaaatgaa tcacctcgct gaaacaatcg agaatcctgt
    46501 cctaggaaaa acaatccata atgttacatt gatcagtggc gggaatcaag tcaacagcat
    46561 cccaagccat gcgcagctcc aagggaacat tcgatcgatt ccagaatacc caaatgataa
    46621 aatcattgca ttgctgcaat caatcgtcaa tgaattgaat caagagacag actatcattt
    46681 ggaattgatg atcgattaca acaaaatacc tgtcaaagct gatccagatt ctcctttgat
    46741 tcattgcatt cagcaacaat tcagccagcc attaccttta gttggagcgg cagcaacaac
    46801 ggatgcagcc gaatttacga aagctaacca ttccttcgac tttgtcgtct ttgggcctgg
    46861 tgtcgttact ttgcctcatc aagtagatga gtatgttgaa atcgataatt atttggatat
    46921 ga
//
LOCUS       DAYYHX010000003        42677 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000003 DAYYHX010000000
VERSION     DAYYHX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.003"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..1134
                     /locus_tag="ACHA6Y_000096"
     CDS             88..1134
                     /locus_tag="ACHA6Y_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986224.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            1136..2395
                     /locus_tag="ACHA6Y_000097"
     CDS             1136..2395
                     /locus_tag="ACHA6Y_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8986225.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            2401..2982
                     /locus_tag="ACHA6Y_000098"
     CDS             2401..2982
                     /locus_tag="ACHA6Y_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM8986226.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(3064..3939)
                     /locus_tag="ACHA6Y_000099"
     CDS             complement(3064..3939)
                     /locus_tag="ACHA6Y_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8986227.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            4087..4419
                     /locus_tag="ACHA6Y_000100"
     CDS             4087..4419
                     /locus_tag="ACHA6Y_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8986228.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            4531..5739
                     /locus_tag="ACHA6Y_000101"
     CDS             4531..5739
                     /locus_tag="ACHA6Y_000101"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM8986229.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            5893..6348
                     /locus_tag="ACHA6Y_000102"
     CDS             5893..6348
                     /locus_tag="ACHA6Y_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986230.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            6710..6985
                     /locus_tag="ACHA6Y_000103"
     CDS             6710..6985
                     /locus_tag="ACHA6Y_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986231.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            6998..9076
                     /locus_tag="ACHA6Y_000104"
     CDS             6998..9076
                     /locus_tag="ACHA6Y_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8986232.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            9239..11122
                     /locus_tag="ACHA6Y_000105"
     CDS             9239..11122
                     /locus_tag="ACHA6Y_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8986233.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            11134..12081
                     /locus_tag="ACHA6Y_000106"
     CDS             11134..12081
                     /locus_tag="ACHA6Y_000106"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8986234.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            12101..12628
                     /locus_tag="ACHA6Y_000107"
     CDS             12101..12628
                     /locus_tag="ACHA6Y_000107"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8986235.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(12691..13344)
                     /locus_tag="ACHA6Y_000108"
     CDS             complement(12691..13344)
                     /locus_tag="ACHA6Y_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM8986236.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            13476..14318
                     /locus_tag="ACHA6Y_000109"
     CDS             13476..14318
                     /locus_tag="ACHA6Y_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8986237.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            14476..15372
                     /locus_tag="ACHA6Y_000110"
     CDS             14476..15372
                     /locus_tag="ACHA6Y_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8986238.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            15375..16088
                     /locus_tag="ACHA6Y_000111"
     CDS             15375..16088
                     /locus_tag="ACHA6Y_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM8986239.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            16108..16626
                     /gene="msrA"
                     /locus_tag="ACHA6Y_000112"
     CDS             16108..16626
                     /gene="msrA"
                     /locus_tag="ACHA6Y_000112"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8986240.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            16623..16850
                     /locus_tag="ACHA6Y_000113"
     CDS             16623..16850
                     /locus_tag="ACHA6Y_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM8986241.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            17002..17553
                     /gene="lepB"
                     /locus_tag="ACHA6Y_000114"
     CDS             17002..17553
                     /gene="lepB"
                     /locus_tag="ACHA6Y_000114"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8986242.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(17636..18178)
                     /locus_tag="ACHA6Y_000115"
     CDS             complement(17636..18178)
                     /locus_tag="ACHA6Y_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8986243.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(18415..18813)
                     /locus_tag="ACHA6Y_000116"
     CDS             complement(18415..18813)
                     /locus_tag="ACHA6Y_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8986244.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            19073..19933
                     /gene="ylqF"
                     /locus_tag="ACHA6Y_000117"
     CDS             19073..19933
                     /gene="ylqF"
                     /locus_tag="ACHA6Y_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM8986245.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            19926..20693
                     /locus_tag="ACHA6Y_000118"
     CDS             19926..20693
                     /locus_tag="ACHA6Y_000118"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM8986246.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            20749..21606
                     /gene="dprA"
                     /locus_tag="ACHA6Y_000119"
     CDS             20749..21606
                     /gene="dprA"
                     /locus_tag="ACHA6Y_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM8986247.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            21728..23806
                     /gene="topA"
                     /locus_tag="ACHA6Y_000120"
     CDS             21728..23806
                     /gene="topA"
                     /locus_tag="ACHA6Y_000120"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM8986248.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            23775..25151
                     /gene="trmFO"
                     /locus_tag="ACHA6Y_000121"
     CDS             23775..25151
                     /gene="trmFO"
                     /locus_tag="ACHA6Y_000121"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM8986249.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            25349..26254
                     /gene="xerC"
                     /locus_tag="ACHA6Y_000122"
     CDS             25349..26254
                     /gene="xerC"
                     /locus_tag="ACHA6Y_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM8986250.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            26291..26839
                     /gene="hslV"
                     /locus_tag="ACHA6Y_000123"
     CDS             26291..26839
                     /gene="hslV"
                     /locus_tag="ACHA6Y_000123"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM8986251.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            26853..28253
                     /gene="hslU"
                     /locus_tag="ACHA6Y_000124"
     CDS             26853..28253
                     /gene="hslU"
                     /locus_tag="ACHA6Y_000124"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM8986252.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            28271..29062
                     /gene="codY"
                     /locus_tag="ACHA6Y_000125"
     CDS             28271..29062
                     /gene="codY"
                     /locus_tag="ACHA6Y_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM8986253.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            29144..30019
                     /locus_tag="ACHA6Y_000126"
     CDS             29144..30019
                     /locus_tag="ACHA6Y_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8986254.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(30151..30789)
                     /gene="plsY"
                     /locus_tag="ACHA6Y_000127"
     CDS             complement(30151..30789)
                     /gene="plsY"
                     /locus_tag="ACHA6Y_000127"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM8986255.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            30916..31350
                     /locus_tag="ACHA6Y_000128"
     CDS             30916..31350
                     /locus_tag="ACHA6Y_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM8986256.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            31516..33561
                     /gene="parE"
                     /locus_tag="ACHA6Y_000129"
     CDS             31516..33561
                     /gene="parE"
                     /locus_tag="ACHA6Y_000129"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM8986257.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            33573..36023
                     /gene="parC"
                     /locus_tag="ACHA6Y_000130"
     CDS             33573..36023
                     /gene="parC"
                     /locus_tag="ACHA6Y_000130"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM8986258.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            36300..38531
                     /gene="pflB"
                     /locus_tag="ACHA6Y_000131"
     CDS             36300..38531
                     /gene="pflB"
                     /locus_tag="ACHA6Y_000131"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM8986259.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            38775..39536
                     /gene="pflA"
                     /locus_tag="ACHA6Y_000132"
     CDS             38775..39536
                     /gene="pflA"
                     /locus_tag="ACHA6Y_000132"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM8986260.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            39621..40547
                     /locus_tag="ACHA6Y_000133"
     CDS             39621..40547
                     /locus_tag="ACHA6Y_000133"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM8986261.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            40729..41622
                     /locus_tag="ACHA6Y_000134"
     CDS             40729..41622
                     /locus_tag="ACHA6Y_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM8986262.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            41756..41881
                     /locus_tag="ACHA6Y_000135"
     CDS             41756..41881
                     /locus_tag="ACHA6Y_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM8986263.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(42015..42647)
                     /locus_tag="ACHA6Y_000136"
     CDS             complement(42015..42647)
                     /locus_tag="ACHA6Y_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986264.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT        14929 a         7308 c         8954 g        11486 t
ORIGIN      
        1 tgcaagttat gaggtgagct ttgctcttat cttttctaga atttgcttac taatggtaaa
       61 ataaaaataa gaagaggagg gatgcgtatg gaacttgatt atcaagtaga agatgcgttg
      121 acgaaagtgt tttacggaga aacgctagtc agtcagctaa gagaactttc cattgatcag
      181 ccaatcttat ttttaacgaa tcagcgctat tatgatttgt ttgcagataa gatcaacccg
      241 ttatttgcaa atcaagcaaa caatgactgg tatatttgtg ccaacactca atgcaatcat
      301 ttaacagaat taaagaacct gcttgatttc gtcaaacgct atcccgaaaa tcaatccatg
      361 tttatcatcg gatttggaaa tgaagggatc atggatctgg caggtttttt tcaaaaacat
      421 acaacccttg attcatcact atggatgatc cctgtatcga tccgttctct aagccgagca
      481 ttaacaccgc agcggacgat tttgcagcta ccaaatcgtg tagtgctgca aaccatcaat
      541 ctgccagaac gcatcgttta tgatcaaaca atcagcgagc ggcaggtgga cggaaagcag
      601 attgactttt tgacttttat ctgttgcgga atgatttgtg atcatcgttt tttgcaaaat
      661 ctgtataaaa actatccaaa taaaaagcaa ttaacgactc ggccgtttgc cgggatgatc
      721 gaagaaatga tctattttta tcaattagag gcagaaaacc tgtcaaatta tggtaaactg
      781 tttgaacaag ctttttatct gaccgaaaat ggccatctac tttctgctag tatgaaaaaa
      841 tttttaggaa tgatgtttca tttcgtgtgg aatctccaag catatccttt tgatttccaa
      901 ctaaaaaact tttttgtgtg gctggacttg cttggctatc caatccactg gctggaacag
      961 atttcaaaga tggagtattt ggaaaaagtt ctttttttgg cagaaaaaag caaaaaaata
     1021 cttgttttag aaaaaatagg tataatagac ggataccaac atccaaacga aaaagaatta
     1081 ttgagaacaa tgacggtatt tcaacaaatc gtcagtgaga taagagggaa ttgaaatgaa
     1141 tacaaatagc gaaaaaatgt tgcaagcttt atcagatgaa gacttgtcac aagcacaaat
     1201 ctttttagaa aaagcattaa aagaagaccc agcagatata ttagctgaac taggcgaaga
     1261 attgctggct attggctttt tgatcgaagc aaaacagatt tttgaacaac tgatcaacca
     1321 atacccagaa aatgacggac tgaacatccc gttagcagaa attgcaatcg aagataatga
     1381 gatcgactta gcttttgaat acctggaaaa agtggataag caaagtgatt actatccgca
     1441 aagcttattg gcgatagctg atttgtacca agttatcgga atccctgaag tgagtgaagc
     1501 aaaattgaaa gaagcagctg ggttgcttcc tgatgaacca ttgatccagt ttgcgctagc
     1561 tgaattgtat ttttcagttg atcgtttcaa agaggcagca gtggtttatg aaacattgtt
     1621 ggcaagcggg ataaatgaaa tttcaggaat ctcgatgcag gaacgtttag gacaatcctt
     1681 aagtatgcaa ggagaattcg aagcagctat cgtaccttta gaagaagcac tgaaagaaga
     1741 aaggacagat gatcgactgt tccagctagc ttttacgaat cttcaattga aagaaaatca
     1801 gcaggcaatc aactatctgg aagaacttcg agaagttaat ccgcattatc aatcgttata
     1861 tctatatcta ggacaagctc ttcaagaaga agaaatgatt gaagaagcac aaacagtgtt
     1921 agaagaagga atcaaagaaa atccttacca agtcgaactt tatcacctgg cctcggaaaa
     1981 tgctttccgt ctgcatgata gacagcgagc agaagaactt ttactgaaag ctttagaagt
     2041 aggagacaaa caagatgaaa cattacttac tttaagtaat ctttacttgg atgatgaaag
     2101 atatgaagat gtgatacaag tgatcaatca gatggaagaa actgctaatc cttatgcaga
     2161 atggaaccta gcccatgctt ataatgaatt agaagacttt accgttgcag ctgtacacta
     2221 tgaacaggct tatcatgaac tatcacatga accagatttc ttgaaagagt atgccttttt
     2281 cttaagagaa gaagggcagc tgaaaagagc gcaagaatta ttgacttatt atttaacact
     2341 tgaaccagga gatatggagg cactgtcatt attagatgac ctaacagaga ggtgagaaaa
     2401 atgctaattg atgtacggga aaagaagaat tttttaacgt ggatggtcaa tcatgtatct
     2461 tttagccgaa gagaagtttt ttggatcttg aattatcttg ccaatcatga agcgatttta
     2521 agcaatgtcc actttgttga aggggcgaat tttgcggatc gaggactgca gatcagtgat
     2581 ctatctgttg aaggggaacc catgaaattg tttttgcagg gaaaagaatt tacggacaca
     2641 gaccagattt ttcatgaaat ccgtttgaat tggaaaaagc ctttatatgt ggaatgtata
     2701 ttcgaaaatg cttggcagac aagagaatat ctatctatct tagaagacaa tccattttct
     2761 tcttgggatt cttcagtcag tgaagaagat actgaattaa ttgaacgata ttttcaagaa
     2821 aaagaagaag aggcaaaact aaaaatgctt tatgctcaaa tcgatcaagc actagaagat
     2881 ggagataggg atgctttttt gaaattatct gatgaagtta accgaaagat ccttttgcag
     2941 acacaaaaga aaagtaaccg ggtaagtgaa cataacaatt agttaaaaat agtatagtac
     3001 taaaaaagac aaaggtcagc tggatgtgga atctttcctc atagcctatc tttgtctttt
     3061 ttcttatagt tttttcagta atcttgtctg tggcttcaaa taagtaaagc cttgcccttc
     3121 cacttcatgc acattgatga ttgaaaggaa agctttttcg tcaatctcgt ggacgatcct
     3181 cttagcttca gttaattcac tagagctaac gaccatatag atgatttttt ttgaattacc
     3241 agagaatgcg ccttctccag aaaggaaagt cacacctctt tgcagaccgg tcatcagtag
     3301 tggtgcaatc tcttcgcttt tgttggagac gacaagtaga ccttttgcag aatatccgcc
     3361 atctaagatc gaatcaatca cacgactgaa aacatacgaa acaatcaacg tatacatcat
     3421 acgtttcaag tcgatatagg ttaatgaagc aagtaagacg atgatatcaa atgccaatag
     3481 ggaacgcccc atagtgatgc cgtaattttg ttctaaaatc cgagcgataa tatcactgcc
     3541 gcctgttgtt ccacccatgc ggtagacgat cccgcttcct acacctgcaa ctaaacccgc
     3601 aagtagagaa acgatcagta gatcatgctg taaattgatt tctagtggaa ccctttgcca
     3661 gatccataaa aagaatgaca aagaaagtgt acctagaaca gtataggcca aggaacgttt
     3721 accgaggatc tttcctccta gtaaaatcaa tgggatgttg attaataaag tcgtataagc
     3781 tggatcaata ccaaataaag ctcttaagat caaggtgatc cctgagactc ccccttctgc
     3841 taaatgatta gcgatgttca agtaaacaag accaaatgca taaatacttg ttcctataat
     3901 gataaaaaga acttctttta ctgtttttcg tttgaccata tgaattccag ctttcaatta
     3961 tgtattttca cagaatttcc atttagaaat tccttcctaa atgtagcatg gaaattcgat
     4021 tttctcaatt gctcttatct aggatttgcg ttaaagtttt ggtaagatag ttttggaggg
     4081 aaaaagatgg ataaaaagcg ttctttatac agtatgcagc aggaagtgga tgaatatatc
     4141 cagcaattca aggtggggta tttctctcca ttagctcaga tggctcgatt aactgaagaa
     4201 gtaggtgaat tggcaagaga agtcaaccac acttatggag aaaaaagcaa aaaggcttct
     4261 gaaccagtta actcggtagc agaagaactt ggtgatgttt tattcgttac gatgatcatg
     4321 gctaattcat tgaatatcga cttaactgaa gtttttgaaa aaaacatgga aaaattcaat
     4381 caaagagacc ataatcgctt tgagcgtaag gacgagtaaa caagatgaag tggtcctggc
     4441 cttgaagact ctgaaacgga cttgtcaaac agtcaccatg caactgagtc tttatctcag
     4501 gacaagaaac acgcaggaaa ggttggaaaa atgaaattaa aacaattacc tctggaatac
     4561 caaaaggcaa ttccagttct gaaaaaatta gagaatgcag gatatgaagc ttattttgta
     4621 ggtggaagtg ttcgtgatat cttgctgaac cagccaatcc atgatgtgga tatcgcaaca
     4681 agcgcatttc cagaagaaat caaacagctt tttccaaaaa cgatagatat tgggatcgaa
     4741 catggaactg tgttggtatt agaaaatgat gaacaatatg agatcaccac atttcggacg
     4801 gaatcaactt atcaggacta ccgtagacca gatcatgtcg aattcgttcg ttcgttggca
     4861 gaagatttga aaagaagaga ttttacgatc aatgcgtttg ctttgaaaga agatggagaa
     4921 atcatcgatt tgttcgatgg tctgcaagat ctagaagaac agatcttgcg agctgtaggc
     4981 aatcctcatg agcggttcca tgaagatgcc ttacgaatga tgcgcgggtt gcgttttgtc
     5041 agtcaactag ggttcaaatt agaggaagaa accttctacg ccatcaaaga aaaccacgag
     5101 ctattaggta aaatatctgt agaacgtata aatgtggaat tcatcaagat gctcttggga
     5161 aatgaacggg ccgcgggaat ccatgcattt attgaaacag agtgctacat ttgctgtccc
     5221 ggtttgaaag aagaaggcga agcactttta cgttttgctg aattaccatc cgtcccattg
     5281 aaagaagaga gccaagcatg ggcattattg atcagccaac tggatctaaa agaaaatgag
     5341 attcgttcct ttttgaaatc gtggaagtgt tctaaccaaa tgattaaaga cgttcaagca
     5401 cttgtccaag gactacgaca acgagaaact ggagctttgg aaccattcga attgtacttc
     5461 ttaggcaaaa aacatgcact gcaagtagaa gaattgatga tctattttga cgaaacacct
     5521 cagatggatg ccgtaggaga atcctatgat cgtttaccta tcaagtcatt aagcgaatta
     5581 gcagtagatg gtaaaatctt gctagaagaa actggaaaaa aacctggtaa gtgggttagt
     5641 gaagctttgc aattagctga aaaagccgtc gtagaacaaa gaataaaaaa tgataaaaaa
     5701 caggttttgg attttttatc gaaagaaaaa ttgatttaaa aaacactaga ctgtatctgc
     5761 tcagctttgt tggctactta aagtgggcag atacgtcatt agaagaaaaa acataagatg
     5821 tgaccactta cataaatttg tgctaaactg aggtagtaca agaataaatt acatcataaa
     5881 ggagtctttt taatgagtaa agaaatgaca gcgctaaaat tttattttag aaatggagaa
     5941 acctggacga tcaaccgccg tcatatcggc gatctttgga tcaaacaaat cacaacaagt
     6001 tttggacgaa tcaatggcag tgaatttgtt gaaatccatc cgtgtgctgg atttaaaatc
     6061 gaaatcttcc atgaagggga cgcagttgcg acacatgata tcaacctagg cggactggaa
     6121 atgggcatgt ttaatcgcgc attgaaatac gaagatatcg agcgaatgga aatcctttac
     6181 cgaaatggca cacctgatct tgtgtacttc ccatatctag ataaaggaac agaaggatta
     6241 gacaatcaat atcaatcaac aaaaatcagc gaaaaaacag gtaacttgta tatcgtgatc
     6301 aatccagatc aacgtgtaga agatgtatac ggggaatttt tcgaataaat cgatttttac
     6361 gcaaaaatga ggctgtgaaa gagatgtgtc ttattactac tgataggctg tctcttccac
     6421 accgtttatt cagaataagg gactgtgaca agatttttgt cgcagcccca tttttttgtt
     6481 gtgataaaag aacaagaaag gcaggaatgg gaaagtaaac atccttttgt cagaaacttt
     6541 tatctttctt aagttttact tgatatctga tactatttgg aatagaatga ggcgttgaaa
     6601 aatttatgga aatttctaac tagcaataaa cgatgaaaca atgaaatatt tttcatccaa
     6661 aaagaacttt gctataatga tggaagtttg tgtataggag atctggaaaa tgagagagag
     6721 ttttgaacaa caaaaaaaac tgttgcatga tcgctatggc gccctttcga tggatgaccg
     6781 ccgtcaaata ctttgtaagt tgcgtaaaag aaatatattg atgtatcgtc agttggaacg
     6841 tctgaaacat gatctgcttc gtttggagtc aaaacgtgta cagtgtgaat tagaggggaa
     6901 ccaaacacag gttgaagtag tcgaaaccaa aattttaaag aaaaaagaac aatttttaaa
     6961 aatgcttaca caaaataaaa aataaagggg atggacgatg gaatttgctg agttagttat
     7021 tgtatttgca gtaacgatca ccttatcgaa catagctagc cgcattttgc caatgattcc
     7081 agctccactg atccaaattt tccttggagt ggtattaggt ctgacagagt ggggacaatc
     7141 aatcgatttt gaacctgaat tgttcttagt tatgattatt gcaccattgt tatttagaga
     7201 aggagaaaaa gcagatattt cttcgattct caaaaatttc gggacaattt tgttcttggc
     7261 atttggagga gtcattttga cccttgtcgg ggtaggtgcg acgttatcat tcctgttgcc
     7321 aagtgtccca cttgctgcct gctttgcttt tggagcagcg ttaggtccga cagatgcggt
     7381 agcagtcagt tctctttctg gacgagtgaa tatccctaaa aaggcgatgc atattttgga
     7441 aggagaaggt cttttgaatg atgcatctgg tgttactgcc ttccagtttg ctcttggtgc
     7501 gctgatcacc ggatcttttt cagcagtaaa cgcggggatg agtctagtag tttctagtat
     7561 tgggggagct ttgatcggct ttttgcttgt ctggttcaaa caaaaaataa ttcatttgat
     7621 cgaaaaagca tcagcacaag atgtcacagg gtatctgctg atcgaacttt tgcttccttt
     7681 tctagcctat gtattggcag aggcatttga tgtatctgga atcattgctg cagtggcagc
     7741 aggtattctc caagcttctg gttttaggaa aatctctgtg tttgatgcag aattgtcgag
     7801 cttgtcccac agtacatgga caactattgc ttttacactg aatgcattag tatttatatt
     7861 tttaggaatc gaattgacgc aagtgttctc gccagtatgg ggagacggtt tatatccaaa
     7921 cggtctttta ttagcgatta tcgtattaat ctctgtcatg ctttttgtga tcagattcat
     7981 ttctatcagt cttttttatg tattcaaaga tggcagtaaa aagttcaaaa aacagttgaa
     8041 cgagatcttg atcttaacat ttggcggtgt aaagggaaca gtcagtttgg ctacgatttt
     8101 tattttgcca ttttcaatca ataatatgat gttttaccag cggtctttgc ttttgtttct
     8161 gacggcgggt gtcatcttag tgactttggt catagggatc attgtcttgc ctatgctgac
     8221 ggaaacagag gaagcgaaaa gcacggattt gaatgctttg atgattttag aagaggtcgt
     8281 tgaagttttg cgaaaagaaa taaaggaaat tgatcgtaat acaaaagaat ttttagccac
     8341 tgaagcagtg atagaaaatt atcaagaacg gatacgtgat ctgtacttgg aagatttaac
     8401 cgatgatgaa aagcaagaag tacaagaaat acaagcactg attttgtcga tcgaacggga
     8461 tggattggat gaaagttatc gttcgggcaa gctttcttct aatggttatc gtttttactc
     8521 ccgttttctt tctcgattcg aacattctat cacaagccag attttgtctt ttatcggctt
     8581 ttggttcatt gttgtccgta ggttaatgcg aatcgttctc catccgaaaa tgttttggca
     8641 aagaagatat gctgatcgtc agacatttat tagtgatgaa gatatccaag aaatcagaga
     8701 gacatatcaa aaaaatactc agttgattat tgaaagttta gataacttga ctgatattta
     8761 tgatgaaacg atggttcaat tttttatcca gcaacgcaaa cttgaaggaa taaaaatgaa
     8821 ctcggggaat ttgatttctt cgtggatgat ccagcaagat agtctgttta cgaaaaaaat
     8881 gctaagaggt tattatcttg aaagaaaaat cattgatgaa tacgaagttg ctgaaaaaat
     8941 cacgacgttt tctgcgaaca gctatcgcag gaatatcaat ttgttagaat cttatacgat
     9001 gaacaaacca tctgataact tttctttccg atttgcgttt cgagcaaagt caaaaaaaac
     9061 gagtccaata aaataaaaag gtaaaaattt tcaagtttta taaaaaacaa aacatatcag
     9121 aacaggatcg aatcggtcct gttctttttt tgagaacaca aaaagaacaa ataccaaaaa
     9181 aattttcctg aagttcacta tttttatgat acaatatcca agataaggag ggaacatgtt
     9241 gaaagaatta aaagtaactg acctcaaaaa aacttatggt gaaaaagatt tgtttgacca
     9301 gatttctttt ttggtccacg aaaaagaccg tatcgggttg attgggacga acgggacggg
     9361 taaaacaagt ctcttaaata ttattgccgg tatcgatagc agcgacggtg atcggcaaac
     9421 agtgttttat ccaactgatt accgtattgg ttatctttct caagcagctg aattttccga
     9481 tgaattaact gttctccaag cagtctttca aggaaacagc cccttgatcc aaactgttcg
     9541 agcatatgag gaagcattga tcgaactagg ggaaaacgga gaagaccctg atgtgcaaaa
     9601 acgttatgct aaagcagaag aacaaatgaa taaagaagat gcttggacta cagataccaa
     9661 cgcaaagatt attttgcaaa aattaggaat cagtgaatta gataaaaaaa tcagtacttt
     9721 atcaggtggt cagaaaaaac gagttagtct ggcgcaagtg ttgattgatg aacccgattt
     9781 actattacta gacgaaccaa caaaccattt agattatgaa gcgatcgagt ggttggaaaa
     9841 ctatttgaaa caataccgtg gcgcactatt gatggttacc cacgatcgtt attttctaga
     9901 tagagtagca aaccgaatct tcgaactttc atttggaaaa ttatacgagt ataaaggaaa
     9961 ttatgaagct tacgtcttag aaaaagcaga acgtgatcgt gtagaagtag aacaagaaga
    10021 aaagcgcaag cggctttata aacaggaatt agcttggatg agggctggcg tacaggcacg
    10081 tggcaccaag cagcaagcac ggatcaaccg tttcgaagat ctaaaggaga acttgtacca
    10141 agtaaaccaa gaagatgatt tagagctgaa cctagcgaca caacgtttag ggaaaaaagt
    10201 actggaaatc aaagatggca gttaccgtat aaatgaccaa acactgctag aacatttaga
    10261 tttattgatt caatctcggg aaagactagg tattactggt aaaaatggtg cgggaaaatc
    10321 gacattgctt aatattctag ctggcagaat tcccctggat agcggtacaa tgtctatcgg
    10381 tgaaacggtt catcttgcgt attatacaca agaaaatgaa gaaatggcac cagataaacg
    10441 gatgattgct tacttacaag aagctgctga agaagcaaaa acagcagatg gcagtcaaat
    10501 cggagtagca gaattacttg aacgattttt atttccaaga tttatgcatg ggacactgat
    10561 aggcaaactt tcaggcggag aaaaacgccg gttatttttg ttgaaactgc tgattcagca
    10621 acctaatgtt ttattattgg atgaaccgac caatgatttg gacatagcaa cattgacgat
    10681 tttggaagat tattttagaa gttttccagg tgctgtgatc actgtttccc atgaccgtta
    10741 tttcttggac aaggtagcag ataagttatt agtattccaa ggaaacggaa aacaagaact
    10801 ttattacgga aacatgtcca gttatttact gaagcaaaaa gagacccagc agcctgctga
    10861 aaaagcgaaa ccaaaaatac agagcaagga acctgctgaa aagaagaaat tatcttacat
    10921 ggaacaaaaa gaatgggaaa cgattgaaga tgagattgca gaacttgaag aaaaaatctc
    10981 attgctccaa gaagagatga atcatcaagg agataatttc actcgattac aagaattgca
    11041 aaacgacgta tctgaaacag aagcacaact tgaagaaaaa atggcacggt gggaatactt
    11101 gagtgaatgg gtagaagatt aggaggaaaa aggatggaac aagcgtatct tgacttagga
    11161 agaaaattgt tggaagaagg gcatcgtaaa gaagacagaa caggcacagg cacattaagc
    11221 ttattcggat accaaatgcg gtttgatctg caaaaagggt ttcctttgtt aacaacgaaa
    11281 cgagtacctt ttggattgat caaaagcgaa ttgctttggt ttttaaaagg agatacgaat
    11341 atccgctatc tattacaaaa taaaaaccat atttgggatg aatgggcatt tgaacgctac
    11401 atcaaaagtc cagactataa tggaccagat atgactgatt ttggccatag atgtttgaca
    11461 gatcctgaat tcaatgaaga atataaaaag cagagcaagc ttttttgcga gaagatccta
    11521 accgatgatt cctttgctga aacatacggc gatcttggac acatctacgg ctatcaatgg
    11581 cgtcactggg aaacaaaaga cggtggcttt atcgatcaaa tcaaacaagt gatcgaagca
    11641 atcaaaaaga ctcctgattc tagaagattg atcgtttctg catggaatcc ggaagatgtt
    11701 cctagcatgg ctttgcctcc ttgtcatacg atgtttcaat tttatgttca agagggacgg
    11761 ctaagctgcc aattgtatca acgcagtggg gatgtgtttc taggcgttcc gttcaacatt
    11821 gcaagttatg cgttgctgac tcacctaatt gcacatgaaa caggtttagc ggttggcgaa
    11881 tttgttcaca cattaggaga tgctcatctt tatatcaatc atatcgaaca aatgaaagaa
    11941 caattgagcc gagaaatacg cgcatttccg actttagttt tgaatcagga aaagcagtca
    12001 gtatttgatt ttgagatgga agatatccaa ttagaaggat atgacccaca tccggcgatc
    12061 aaagctccta tcgctgttta gaatcagaaa ggatgagaaa atgttagctg caatttgggc
    12121 acaggatgaa aacggattga ttggaaaaga agaccgtctt ccttggcgtt tgccaaatga
    12181 tctgaaattc ttcaaacaaa tgacagaagc aaatacacta gtgatgggac gaaaaacatt
    12241 cgaagggatg ggtagccgtc cgttgccgaa tcgtcagacg attgttttga cgagagattc
    12301 ttcctataaa gcagaaggcg tgcatgtcat gcatagtgtg gaagacgtgc ttgctctgga
    12361 aaaagaaaca gacggtatct ttttcatcgc tggagggtca gctgtttatc aagaatttct
    12421 gccattttgt acgatattat accgtacagt catccatcat gtattcgatg gagatgctta
    12481 ttttccacct gttgactggt ctgattggtc actgatcaat actagtcaag gagaaatgga
    12541 tgaaaaaaat cgttatccgc atcaatttga aacgtatcag cgtaatgaga atcctgtaaa
    12601 aagcgggaca ggcgaattga cgatataaaa aagctatgga tagactagct gttatttaga
    12661 gctgtcccat ccatagcttt atttttttta ttaagtaaag aataaaacag agaagtacat
    12721 aaaacctgca cctaacataa caaacaaatg ccaaacaaca tgcatatagc ggatagaatt
    12781 caagctataa aagaatgcgc ctatcgtgta agaaagcccg cctgctacca tcaaaataac
    12841 accagtcatt ccaagagagt gataaagagg aacagcagcc acgacacaga gccaccccat
    12901 aaaaatatag atgatcgtag atacttttga tactctctcc cgtttatgca acgtcaacgc
    12961 tttatatata atgccaagaa tcgcaaacaa ccagattaat acaaataaag tccaacctag
    13021 ccatcctcgg atactcagca aacaaaaagg agtataactt cctgcaatca aaagaaaaat
    13081 cgaactatga tcgaatactt ggaacacctt ttttgctttt gtaaaaatca atgaatgaaa
    13141 caaagtagac gttaaaaata aaagaatcat catcgatcca tagatagcat aagagacaat
    13201 atggagggag gaatgcaagt gggcaccttt tactaaaaga accactagtc ccgcgatact
    13261 caatccagtc ccaattccat gggtgactgc attaaacact tcatttaata tttcatacgt
    13321 tcgattaaat ttcgtttttt ccatgaaaac ggctccttta ctaggtgtaa ttaatgaaaa
    13381 atcattattt gtctgttata ctaaaaatag aaattgtctt acatgtagtt taacatgaat
    13441 aaggcatctt gttaaagaaa gagagagaga aaataatgac aaatgtaaaa atcgtaacag
    13501 attcttcatg tacgatggaa aaaaatgtac gcgatgcttt aggcattcgt gtcatgccgc
    13561 tatccatcat gatcgacgga acagtatatc ccgatgatga ccaactagac ggcgaaagat
    13621 ttatggaaat gatggcgaaa gcaaaaaatc tgcccaaaac aagccaacca ccaattggag
    13681 aattcatctc gatgtatgat gagctaggag aagacggcag tgaagtgatt tcgatccata
    13741 tgacaaaagg cttgagtgga acagtagaag cagcaagaca agccagtaat ctaacaaaaa
    13801 cgaaagtaac ggttatcgac agtgatacca ctgaccaagg attatctttt caggtaattc
    13861 gagcagctga attagcaaaa gaaggtaaaa cagtagagaa aatccttgaa gaagtcaaaa
    13921 aagtacaaga caatacaaaa ttatacatcg gaatatccac actagataac ttggtaaaag
    13981 gtggaagaat cagccgaaca actggtttgc tgtctagtgt tttgaacata cgcgtggtga
    14041 tgaatttcgc ccattctgaa ttaatccctg taactaaggg gcgaggaaag aaaacgttcg
    14101 ataaatggtt taataatttg aaaaatgaac tgaaagaatt gccaaatgtg cgacaaatcg
    14161 gtatttcaca tgcggatgca aaagaattgg cgcaagaatt caaagagggt ttgcaagaat
    14221 tattcccgga catgcatatc cctgtactgc atacgaatcc ggttatcgct acgcatactg
    14281 gtaaagacgc gtttgcaatc atgtattatg tagattagtc gaaaagagga taagccctca
    14341 gcctgaaagg cttgcagctt attctctttt tctttataaa attaggaaaa taattgtttt
    14401 cttttttcat aaagactacc tttttgataa aatttttgat aacattagaa ccaatcgaat
    14461 acaagaggtg aaaaaatgaa attaacgaaa cgacatttga tcctcgtttt gttcccatta
    14521 ttaattgcga tcatcatcta tgcaggttta gctgcttcta taccgaaagc tggcccacta
    14581 atgaaaccag taactactgt gagagaagat ccaaataaaa aagaaaaact tcactacata
    14641 gctatcggag attcgctgac tgaaggagta ggagatcaaa ccaagcaagg aggattcgtt
    14701 ccccttgtcg caaatgatct aaaagaccgt tataatttga ccgcggttga aacagaaaac
    14761 tatggtgtaa atggtgaacg aagtgaccag atcttaaagc gagtaaaaac aaaagaagaa
    14821 attcgaaaca atatcaaaac agctgatttt atcacactga ctgttggcgg taatgatttg
    14881 atgaaagtta tccaaaataa tttattcggg ttaacggtca attcctttaa aaagccactg
    14941 aaaaactatc aggaaaatgt cacagaacta ttggaagaat ttcgatccct caataaaacc
    15001 gcacctattt acattttagg catctacaat ccattttatt tgaactttcc tgagatcact
    15061 gacatgcaga caattgttaa caattggaac aaaggaacag aaacggtgac aaaaaaaatg
    15121 ccgaactgct actttatccc aatcaatgat ttgctttatc aaggattgaa tcaggaagta
    15181 ggtgtcacag aagaaggaac agatcaaaca actagcacga cagaaaatct aaatggcatc
    15241 aaaaataatg tgctctatga agaagatcat ttccatccaa ataatttagg ttatcaattg
    15301 atggcaaatg cagtgagaga tcagctgatc gagacacaaa accaatggtt agagaaggag
    15361 gatgatcaat aaacatgaaa acaagatctg acgtatcttc aaacaaaaca aagaaaacaa
    15421 attactggaa attcgccttc cttatccttc ttggggtagt acttggttcg accgtatttt
    15481 taggcagccg tatttttgct aatcgggaac cagaactccc agaaatacca gctcttacgg
    15541 aaagacaagg agatcctgtc ttaacgatca attcaaataa agaaaaagtc aatcaaatca
    15601 tcagcttttt ccttagtgag tatcaaaaag atagtgatat tgaatataaa ttttatttgg
    15661 aaaatgaagc actactcaat ggtactttcg aagtactagg ttttcctatc gatttctatt
    15721 tatattttga tccgtatgtg atggaaaacg gaaatgtcca actaaaagct agaagtcttt
    15781 ctatcggaac attgagtttg ccaatcaaag atgtgatgaa tatgatcaag agaaactata
    15841 aattaccaga ttggatcgaa gtcaatacag atgatctgac tatcatgctt aggttggatc
    15901 aattccgaat gcaaaacggg atgtatatta aagcagataa aatcgactta gtaaatgatg
    15961 acattcggtt tagtctatat ttgccaaaag atacctctga tgcttcaaac aactcaaata
    16021 ccaacaaaac tagcaccagc agtactgaaa gcaagacaaa agaaaataaa aaatcatcgg
    16081 atgagtagta ttagaggggg aatcataatg gaacgagcaa tatttgcagg aggctgcttc
    16141 tggtgtatga tccagccttt cgacacgctt cctggtatcc atacagtgat gtcaggatat
    16201 actggagggc atgtaccaga tccgacttat gaacaagtaa aaagccaaac gactggacat
    16261 acagaagcag tggagattat ctatgatcca acattgattt catatgaaca attagtagaa
    16321 ctttactggc agcaaactga tccaactgac gctttcggac aattcgagga tcgcggtgac
    16381 aattatcgcc cggtgatctt ttatttgaca gaagaacagc ggatcattgc tgaaaaaagt
    16441 aagaaacagt tagctgaaag tggacgtttc gaacagccaa tcgttacaaa aatcgaacca
    16501 gcccaaccat tttatctagc agaagaagac catcaagagt attataaaaa gcatcctgaa
    16561 aattttaaaa gaaaccatgc acgccgggct gcctttatcg aagaaaattg gaggaacgaa
    16621 gggtgaatcg aagctttttt cattatttaa tgacactgcg aggcggtggc acacatgatg
    16681 ctttatctgt tttcgctaca gaagccggta aagacatcca atttcctaag cacagctcta
    16741 gctacgaaga aatttctaat tatctggaac tgaatgtcga ctatttgccg tccatggata
    16801 tctttgacga agcatgggaa aaatatttag aaaataatca tgacatataa gagatagttt
    16861 tgtactttat gatcaaaaaa gttttttgtc caatagccta attgaaagaa ttatttttat
    16921 ttcagctatc tagtcgcatt tttttgatat ttttcttata ataaagaaag aatgaaaaaa
    16981 agagggatgg attgatagag attgacaaaa aaacaacgct atattaatcg cttctggctt
    17041 gtattcaaat acttacttgt ttctgtattc cttgcgttca tgttgcgggg atttttattc
    17101 atcccggtac cagtggaagg caattcaatg gaaaacgtgt tgaagcaagg tgatatggtt
    17161 gtgatggaaa aattttcgga gatcagacga tttgatattg ttgtttttca attagcagat
    17221 ggcacgattt atatcaaacg agtaatcggt cttccaggag aaaatgtcag ctaccaaaat
    17281 gatcaattga agatcaatgg gaaagtagtt aaggaacctt atctaactaa aaacctgaaa
    17341 tcagatcatg caaatgcatc ctatacgacg gactttacac tacaagagct aacgggacaa
    17401 tccaagcttc cggaagataa ttattttgta cttggagata atcgaagagt ttcaaaagat
    17461 agtcgttcgt ttggcacgat caataaaact gatattctag gtaaagcaag atttgtctat
    17521 tatccacttg atgaaataaa atggatccag tgagaaacaa taaagcgtgc agtctgttga
    17581 gactgcacgc tttattgttt aatccaaaat cagtttacta ccttagtacg taaaatcaaa
    17641 cagctcgttt aaacactttt ctagcctcta aagttttgat aaatgaataa acaaccatta
    17701 tttgaatagg gatttggatc atattcctga tgaatctacc tagaaaaatt actttgaaat
    17761 ctgcaccaaa caaaacagac atcagccaag aattgataaa aaggctgact gggatcataa
    17821 tcaataattg ggcagcgatg acggaaggca ggtttatctt tttatgatat aaaaataagc
    17881 cattaataaa tccaccaagg aaagccgtga taagaaaagc agcttgaaac gttcctgaac
    17941 caaatatcgt gaaatttaaa gtgttcgaca acatttcaag caaaccagcc cagatcggtc
    18001 catatagata acccataagc gccatactta aaaatccgaa tgtgatttga aaataagcag
    18061 gaccaatcgc tatcttgtat aagatgacat caagggctaa taaaagagca gaggtcgaca
    18121 acaatcttgc attcatttcg ggcaatctaa aaacgtcttt tgcaatcaaa gtgttcaaaa
    18181 tattctctcc tttgtcagtt aataataaaa ggataccata aaatcaccaa tgtgtcatga
    18241 cacattggtg atttacatac actggcatct aacaattaaa aaaagggtga gaagactgct
    18301 tttataaact gtaaaaaaga gattgtacga ataaaaaaaa tagaggcgga gaatacttat
    18361 cgtatcatcc gcctttgttt ttttgttata aaaaacgtca gattagttat ctgcttacat
    18421 cttcatccat acgggttcta atgtatttcc gtctagatct tggacttcta atgaaaacat
    18481 ttcttcttca ggcattccca tatctacttc gtggaaagta ccaccatttt cttttgcaat
    18541 ttgagcaaat ttttttactt catccgcatt ttcaacacta aacgaaatca aagcaccact
    18601 tgttttttta ttatcagcta tttctttatc ttttataaat tttttataaa aatcatgaga
    18661 gagaagcatt atccaaaaag tgtcatccca taccattgaa ccagcttctt cagtagaaaa
    18721 ttcttcattt tttttaaaac ccagcttttc ataaaaagca acggaacgat tgacatcagc
    18781 aacaggaaaa ttaacaaaaa ccatactatt catttgtttc gtctcctttc tgttttgttc
    18841 gtctatcatc tcttatttag gaaacgctgt caaaaattta gattcatata tagaaaccaa
    18901 aataaaaatt tgcatcatca aaaaagtgaa gatgtgatag aatgaggact ggttcacttt
    18961 cacttaacta atagatggaa tttctttata ataaaaaagt gaatttatat tgaaaaagaa
    19021 aaaaacaagt aaagccgggg aatattccgg tatgaaagga atggagagaa caatgaccat
    19081 ccaatggttt ccgggacata tggcaaaagc ccgacgagaa gtttctgaaa aaatgaaata
    19141 tgtggacatc gtatttgaat tggtagacgc acgattacct ttatcttctc gtaatccaat
    19201 gctagatcag atcattcagc aaaaacctcg ccttgtttta ttgaataaag cggatctggc
    19261 agatccacaa cagacaaagc tgtggcaaga atattttcgt aaacaaggcc atcttgcatt
    19321 agctatcacg gcacaagaaa gtaaaggaat taaaggtcta gtacctgcgg ctaaagaagc
    19381 attgaaagaa aaacgggcaa gagatgctgc tcgggggatc aagcctcgcg ctattcgggc
    19441 aatggtctta ggtattccaa atgttgggaa atctacgttg atgaatcgac tagtaggcaa
    19501 gaaaattgca cagacaggaa ataagcctgg cgtaacaaaa ggtcagcagt ggcttcgttt
    19561 aggtaatgag ttggaattgt tagacacacc agggatttta tggcccaaat ttgaagatga
    19621 agaaatcggc aaaaaactag cgctgacagg cgcgatcaaa gaccagcttg tccatttaga
    19681 tgatttagcg atttacggat tagacttttt tgcccgatat tattcaggaa gaatcagcga
    19741 tcgttatggt ttatcaatcg agcaagaaga aaaattggca ccggaattac tgatggatat
    19801 tacagcaaaa cgtgggtata aagaagacta tgatcgtggc agcgaattag tcgtgcatga
    19861 tattcgtcaa ggaaaacttg gacgatacac actggatcgt tgcgaagaaa tggagcaaaa
    19921 aagtgatgac tgaatccatc caaagtatca aagaacgatt gaaaagcata cagagaatag
    19981 atgatcctac actagaatca ttaagaaatg atccacgcaa aggcgtgcag caagcactgg
    20041 cttcttttga acgacgattg aaaaaggaag cattaattaa ggaaaaatat gaagaaatgt
    20101 tttgctttga aaagcaaggg tacgcacaag gtcaccaatg gattgccggc attgatgaag
    20161 taggaagagg accattagct ggacctgtcg tggcagctgc agttatcttg ccgaaaaatg
    20221 agatcatatt agggctgaat gactccaaac aattatcaga gaaaaagcga gaacttcttt
    20281 atcaacaagt tcaagaaaaa gccctggcaa tcgggatcgg gatcgttgat cagcagacaa
    20341 tcgatgatgt gaatatttat gaagcaagta aattagcgat gatccaagca gtagaaaaat
    20401 tgtccattgt accaagctat ttgttgatcg atgcgatgac cttggatttg cctattcagc
    20461 aggaaaaaat aatcaaaggg gatgcacgat ctgtgtcgat tgctgctgca agtatcattg
    20521 ctaaagtata tcgagaccaa ttgatgaaag aatatgacgt gctttatcct ggatatggtt
    20581 tctcgcataa tgcaggttat ggaacaaagg aacacttaga aggacttgaa cgacaaggaa
    20641 ttacgccaat ccatcgtaaa agttttgcac ctgtaaaaaa atatttacaa taaaaaacag
    20701 ccgcttttac gtatcttatt agaaagagag acgaaaggcg gttttatgat gtatcaaata
    20761 gaagaaaatt tattgaaact tgcttataca aaaggaatca gtagtcaagg aaaatgggca
    20821 cttgctaact ggatgatgca gtatccttat aaagaaatcg gatttgaaga catcattaag
    20881 ataggtaaaa taaccaaaca ccgggagctg tttcgcaaaa gttggcagga aattcacgct
    20941 aactggggca aaatccaatc gaaacagtcg tttatcactt gctttgatcc caaatatccc
    21001 cctcagctgc ttcacttgac ctatccgcca attgtactgt tttataatgg tgatctatct
    21061 ttactttctt gtaacatgtt aagcgttgtt ggtggaagac acacttctgc tttagccaaa
    21121 aaaacagttt attatttact aaaaccagtg atagaagctg gatatgtgat cgtcagtggc
    21181 tgcgcgaaag ggatagatac atacggtcac caagcagcaa tctctcatgg tggacggaca
    21241 atcgcagtca ttggaacagg gatagagcaa gtctatcctg ctgaaaatcg tgacttgcaa
    21301 gaggtgattg caaatgatca tcttcttttg agtgaatatc ctcctcaatc cggcccgaaa
    21361 aagcattgtt ttccaatgag aaatcggatt atcgcagctt tgtctaaagg aacatgtgtc
    21421 tttgaagcca agaaaaaaag cggctctctg ataacagcac agcaagcatt ggatcttgga
    21481 agaacagttt tttctgttcc aggaaatatt ttaacagaac attctaccgg atgtcatcat
    21541 ttgatccaag acggtgcagt ttgcacggtt tcgggtgaag atatcttggc agaatttaaa
    21601 gaataacacc atacttttca tttgcttttc ttgacaaacg ttttctgaat ggatatgatt
    21661 agctacgatt tgtatgaatc ctcttttaat agtaggaaca cgacaatgga aaggagtcca
    21721 ttcagtaatg gcgtataaat atttagtgat tgtagaatca cctgcaaaag ccaagacaat
    21781 tgagaaatat cttggacgaa actataaagt aatggccagt gttggccata taagagattt
    21841 accgaaaagt aaaatgggta tcgattttga aaacaattat gaaccccatt atatttctat
    21901 acgcggaaaa ggcgatgtca tcaaaagcct gaaagccgca gcaaaaaaag ctcaaaaagt
    21961 ttacttggca agtgacccgg atagagaagg agaagcgatt gcttggcatt tagcgtacct
    22021 acttgggttg gatctgaaag aaaaaaatcg ggtggtcttc aatgaaatca caaaagacgc
    22081 agtcaaagca gcttttaagg aaccaagaac gatcgatgta gatttagtag atgcacagca
    22141 agctcgtcgt accttagaca gaatcgttgg ttattcgatc agtcctattc tctggcgtaa
    22201 ggtcaagaaa gggttaagtg caggacgtgt ccaatctgtc gctttaaaaa ttattattga
    22261 ccgtgaaaaa gagatccgag aatttgttcc agaagaatat tggagcatcg acggtaattt
    22321 taaaaaagct cgcaagaaat tcaaagcaaa tttctgggga atcgacggta agaaaaagaa
    22381 attaccagat gcacaaagtg taaaagaagt cactgctaga attgatggaa cagacttcga
    22441 agttaaaaaa gtagaaaaga aagagcgcaa aagaaaccct gcgaatcctt tcactacaag
    22501 tagcctgcaa caagaagctg cccgtaagtt aaactttcga acaagaaaaa ccatgatggt
    22561 agcacagcaa ttatatgaag gaatcacttt gggtaaacaa ggaacagtcg gcttgattac
    22621 atacatgcgt actgattcta cccggattgc cgatacagca aaagcagagg ccgcagcatt
    22681 tattgaagat acgtatggaa aagaattttc tagacatgat caccgaaaga tcaaaaatgc
    22741 tcaaggcgca caagatgccc atgaagctgt gcgtccaacg agtgtgcttc gtacaccaga
    22801 tgaactcaaa caatatttag ataaagatca attgaaactt tatacattga tctggtcacg
    22861 ttttgtggct agtcagatga caccagctat tttagataca atgaaagtga cattagaaca
    22921 aaatggtgtc atcttcattg cgaacggttc aaaaatcaaa ttcaaaggat tcatgcaagt
    22981 gtatgtggaa ggtagagatg acggcaaaga agagaaagaa aatattcttc cagaacttga
    23041 agaaggcgat gtagtccaat ctgttgatat tgaaccaaaa caacatttta ctcaaccacc
    23101 agcacgtttt agtgaggcta cgttgattcg tgcactagaa gaaaacgggg tagggcgacc
    23161 ttccacttat gcaccaacac ttgatacgat ccaaagacgc tactatgtaa aattgacaca
    23221 aaaaagattt gagccgactg aactaggaga aattgtcaat tcattaatct gtgaattctt
    23281 ccctcaaatc gtcgatatcc atttcacagc agaaatggaa ggagatttgg ataaaattga
    23341 agaaggcacg gaagcgtggg tcaaagtggt tgatcgtttc tataaaccgt ttgaaaaaga
    23401 actgacgaat gcggaagaaa aaatcgaaaa aattcaaatc aaagatgaac cagcaggatt
    23461 cgattgtgat gtctgtggac atccgatggt gataaaatta gggaaatatg ggaagtttta
    23521 tgcttgcagt aattttcctg attgtcggaa tacgaaacca atcgtaaaag aaatcggtgt
    23581 gacttgccca gtatgtcatg aaggacaagt cattgaacga aaatctaaga aaaacagact
    23641 attctatggt tgttcccgtt tcccagaatg tgaattcact tcttgggata aaccagtggg
    23701 cagaaattgt ccaaaatgtg acggatatct ggtcgagaag aaagtcaaag gcggtaaaca
    23761 agtagtctgt gtaaatggag attacgaaga agacatccaa aaataataga aaagaggttc
    23821 tttcattgaa aatggtaaat gttgttggag cagggcttgc cggaagtgaa gctgcttggc
    23881 agatcgcaca agcaggagtc cctgtccgtt tatatgaaat gcgtccagtc aaatcgactg
    23941 aggcgcatca cacaagtaat tttgctgaac tagtatgttc caattcatta agaggaaaca
    24001 gtttggctaa tgcagtaggg ttattgaaag aagaaatgcg ccgattgaat tcggtagtaa
    24061 tcacttcggc agatgaaaca gcagtgccag ctggtggtgc cttagctgtt gaccgcgatt
    24121 ctttttctga aacaattacc cgaaaagtca aagagcatcc attagtaaca gtgataaacg
    24181 aagaaattac tgaaataccg gaaggaatca ccgtagtagc aacaggacct ttgacttctc
    24241 atccattagc agaaagcatc aaagcattca atggttcaga tggtttttat ttctatgatg
    24301 cagctgcacc aatcgtagac aaagctacga tcgatatgga taaagtatat ttgaagtccc
    24361 gctatgacaa aggagaagca gcttacttga actgtccgat gacagaagag gaattcaaag
    24421 ctttccatga ggcactaatc aatgccgaag tggcagaact taaatctttt gagaaagaaa
    24481 aatattttga aggatgtatg ccaattgaag taatggcaca gcgaggattc aaaacgatgc
    24541 ttttcggacc tatgaaacca gttggattgg aagatcctaa aactggcaaa cgtccttatg
    24601 ctgttatcca gctgcgtcaa gacaatgctg cagcttcttt atacaacatc gtgggctttc
    24661 agactcactt gaaatggggc gagcaaaaac gtgttttccg gatgattcct gggttagaaa
    24721 atgctgaatt cgttcgctat ggtgtgatgc accgaaacag ctttatgaat tcgccagaat
    24781 tgctgaaacc gacttaccaa tcaacgaaac gcgaagatct gttctttgca ggtcagatga
    24841 ctggtgtaga aggctatgtt gaaagtgcag cgagcggact acttgctgga atcaacgccg
    24901 cacgcttagc aaaagaaatg gaaccagtcg agtttccaca agaaacagct attggaagta
    24961 tggcttacta cattactcat gcggaaggaa aacatttcca accgatgaat gccaactatg
    25021 gtctgtttcc tgaactgcca gagcgcattc gagacaaaaa agcaagatat gaagcaattg
    25081 cacaacgagc tttaaaagct aatgaggcaa taaaaacaga attagaaaaa actgcaatcc
    25141 caaatgagta aggaaactcc atgaagaggg tgagacggat agtctcgctc tttttcttgc
    25201 gtttttctta agaatttctc aaacaaaatg tgaggaattg cgccaattgt gaacaaactt
    25261 agaaatattg gttaaattct gacaatttat ttgtcttttt ataaacgctg tgctaaatta
    25321 ttctctggga caaaaggaag gtgagaaaat gacagaagcc acgtgggaac aaaaattttt
    25381 gcgttatttg attgtcgaaa gaggatattc agaaaaaacg aaagaagcat atgaagaaga
    25441 cattctccat ttcaaacatt ttttggagga atctggagac gctgatctgc tgaaggcgga
    25501 atattttacg gttcgtgtct atttgagtgc attagctgat catcgtccta gctatagcag
    25561 aaattctatc agtcgcaaga tagccagcct acgttcgttt tatcagtatc ttctcaaaga
    25621 agaagtgata aaagaaaatc cattttctta cgttcatctc aaaaagaaaa atttgagact
    25681 gccacgtttt ttttatgaaa atgaaatgca agcacttttt gacagcgtag caggagatag
    25741 tcctttggat ttgaggaacc gcgctttact ggaagtcctt tacgggtcag gcatccgttt
    25801 aagtgagtgt agcagtttgt cggtatcaga cattgatttt gattcagagg tcatgttgat
    25861 ccatggtaaa gggaacaaag agagatatgc tcctctaggt tcttttgctc aagatgctct
    25921 gcaagaatat ttcagggaag gccgccaggt attgatgaca aaatatcacg aagagcatga
    25981 tatcgttttt gtgaatcatc atggaaaacc aatcacgcct actggaattg aatatgtctt
    26041 gaatcaaatg atcaaaaaaa gcagtttgga tagtgatatc catcctcaca tgctgcgcca
    26101 tacatttgct actcatttat taaataatgg ggcagatttg cggacagtcc aagaattgct
    26161 aggacatgcc aatttatcga ctacccagat ctatgcccat gtaacaaaag aaagtttaca
    26221 aaaaaactat cgttctttcc atccaagagc gtaaaaataa ggaaaagaac aaatttagga
    26281 ggaaacaaac atggtcgaat caccatttca ttcaaccact atctgtgccg tcgaaaaaga
    26341 cgggaaattt gctatggctg gagatggcca agtaacaatg ggtgaatctg ttgtcatgaa
    26401 aggcactgca aagaaagtac gacgtatcta caatggagaa gttgttgtag gtttcgccgg
    26461 aagtgttgca gatgcgttta ctttagaaga aaaatttgaa ggcaaactaa acgaatataa
    26521 cgggaatcta caacgcgcag ctgtcgaatt agctcaagaa tggcggacac agcaatctat
    26581 gcaaaaactt gaagcgatgc tgatcgtaat gaatgataaa gaaatgcttc ttgtttcagg
    26641 tacaggagaa gttatcacac cagatgatgg tatcttagct atcggatcag gagggaattt
    26701 tgcgttggct gcagcccgag caatgaaaaa ctatgctcaa aacgacatga gtgcgaaaga
    26761 aattgcagaa aatgcgttaa atattgccgc agatatctgt atctttacta atcataatat
    26821 tatcgtagaa gaactgtaag aggtgagtag atatgtatga attaaaacaa acaccaagac
    26881 aaatcgtaag tgaattagat gaatatatcg taggacagca cgcagcaaaa aaatctgttg
    26941 ccgttgcttt gcgtaaccgc tatcgccgtt tgcagttaga tgaaaaaatg caacaagata
    27001 ttacaccaaa aaatatgctg atgatcggac ctacaggggt cggtaaaaca gaaatcgcac
    27061 gacgtttggc aaaaatcgtc aatgcaccat ttgtcaaagt cgaagcaaca aaatttactg
    27121 aagtcggtta tgtcggaaga gatgtagaat caatggttcg ggatttagta gaaaatgcga
    27181 tccaaatcgt agaaaaagag cagtacagcc gtgtatacac aagtgctaaa aaaaatgcag
    27241 aaaaacgttt ggtaaaaatc ttggtacctg gtatcaaaaa agaacagaaa caatccacta
    27301 atcaatttga acaaatgatg aatcttttta atgctagtca gcaaaatacc gctgaaccgc
    27361 aagaagaagt gacagaagat attcgaatca atcgccgtac agtcttggaa caactacaaa
    27421 agggtttact ggatagtcgt gaagtaacca ttgaagttga agaccaaaag aaacctgcac
    27481 caatgatgaa caatggattg gagcaaatgg ggatcgacct aaatgaaact ctaggtgctt
    27541 tgacaccgaa gaaaaaagtg gaacggacgg taacagtcaa agaagcttta gagttattga
    27601 tcaaggaaga atcagcaaaa ttagtcaagg atgctgatat ccatagtgag gcaatcaaat
    27661 tagcggaaga aagtggaatt atttttatcg atgaaatcga taagatcact tctaaatcac
    27721 agcaatctgg agaagtatcc agagaaggcg tccaaagaga catattgcca attgtcgaag
    27781 gttcacaagt aaacacaaaa tacgggatca tccaaacaga tcacgtccta ttcattgctt
    27841 caggagcttt ccatttgtct aaaccaagtg atctaattcc agaacttcaa ggacgcttcc
    27901 cgatacgggt agaattagat gatttgacag ctgaagattt tgtgaagatt ctgaccgaac
    27961 caaataatgc tttagtaaaa caatacgtcg cgttgatcgg tacagaaaat gtgtcagtta
    28021 cgtttacaaa agaagcaatt gaacggatcg ctcaaatcgc tttcgatgtc aaccgtgaca
    28081 cagataatat cggcgcacgc agactgcata ccattttgga aaaactacta gaagatctat
    28141 tatttgaagc accagatatg caaatgggag aaataacgat cacagaagca tatgtggatg
    28201 acaaacttgg ctcaattgcg gcaaatgaag atttaagtcg ctatatttta taaaaaatcg
    28261 gaggacgaca atgacagact tgctaacgaa aacccgcaag ataaacacac ttttgcagca
    28321 aaagaatact tttgacatgc aagcagattt gccttacgat aaaatggctg tcacactagg
    28381 tggtattctt gacagcaaca cctatatcat aggaagtgac ggagtattac taggttataa
    28441 tgaacgtcac gatgtgaaca atgaacgaat caaaacaatg tttgttgaga aacaatttcc
    28501 tgaatcttat actgatacag tggcacagtt gatgaaaact gaagctaata tcccaattac
    28561 tagtgatatt actgcatttc cggtagagtc aagagagaaa tatccatttg gtctgacgac
    28621 tgtcgttccg atctttggag ctggcgaacg tttgggtacg attatcttat ctcgtatgga
    28681 acaatcgttt gcagatgacg atctagtctt agcagaatat ggagctacgg ttgtcggtat
    28741 gcagatcctt tatcagaagt ctcgaagcat cgaagcagat gtacggagtg caacggctgt
    28801 ccaaatggca atcaacacat tatcttatag cgaattgaag gctgtacaag ctatctttga
    28861 agcattggat ggagatgaag gtcgtctgac tgcttctaat attgccgata gtatcggcat
    28921 cactcgttct gtgatcgtca atgctttacg aaaattggaa tcagctggaa tcattgaatc
    28981 tcgttcgtta ggcatgaagg gaacttattt aaaagtattg aattcccgtt ttaaagacga
    29041 gttagcaaaa cacagttatt agaagtaagg ggactggggc agatagaaat gtttgttcca
    29101 gccttcccat tttaaaaagt aagtcgaagt tggaggaatg gcaatgactg ttattattca
    29161 aaatgaccag ctgatcgccg aaatcgctga acacggtgcg gaattgatca gtctgaaatc
    29221 aaaagaacat ggtttagagt atatctggca aggtgatcca gcgtattggg gcagacatgc
    29281 accggtttta ttcccttttg ttggacgttt aaaagatgat cagtatgttt atcaagggaa
    29341 aacatatcaa atggggcaac acggatttgc tcgggatatg gattttgaag tcattgaaca
    29401 tggacaagaa atggctagtt tcatcttaag gagtacgccg gagacattag aaaagtatcc
    29461 atttgatttt gaattagtga tcagctatga attaggagga gacggcatca cggttcatta
    29521 tcaagtggca aataccggtg aaaaagaaat gttcttttct atcggtggac atcctgcttt
    29581 caatgtacct cttgaagaag gattgtcatt tgaagatttc tatctggcat tttctccatt
    29641 gaaatcaaga ttacgcctac cattgaaagg cccattcatc gatatggatc aaaagacgtt
    29701 aggtcagaca aatacgaatc tggctttgaa tcgtgaactt tttgagcaag atgccttggt
    29761 ttttgaaaca aggggtctaa atgcgtacgc cattcgaagt gaaaaatctt cccatagcgt
    29821 aactttaagc tataaagata tgccttatgt agggatctgg tctccttatc caaaagaagc
    29881 gcctttcgtt tgtatcgaac cgtggtgcgg aatcgctgac acggtgaaca gtagtggaaa
    29941 cttgatggaa aaagtaggga tcaatcaact aggtgcccat gaaatcttca agacaaaata
    30001 ttccatcaca gtgaagtaag aggttgtgac cgagaagtta gcagggaaca ccgtttacca
    30061 aggtgtgtaa aaaagctatc ctgtaagtaa taagggagtg tgacatcgat ttgtcacact
    30121 cccgttttat ctctttggaa tgaaaaagta tcaggatttt ttcttgctgc tattcaaacc
    30181 aaacgggatt cgactttcag tatggtttct gatacgcttg atattgtctt tatgccgata
    30241 aaagataaag gaagaaacta aaaaggcaat gatcgtcagt aaccaattgg gcttagccaa
    30301 aatcacagga gctacataag gaaggacgat cgtagataaa gtgatcaata ctgcactgat
    30361 catactgctc aaactcacca tacttgtaca aaataaacta acgataaaga tgatacaaga
    30421 ataaacaaaa aatacaggat taaatcctaa aagcattcct gcacttgtcg caacagcttt
    30481 tcctcctttg aacccagcaa aaatcggaaa tgtatggcca agaatggcgc ataccccaaa
    30541 ccaaagaggg ttaatcccag aaaggtggaa gatcatcggc aaacaagtag ccaatgtacc
    30601 ttttaaaata tccataagca ggacaacaat cccggctgtt ttgcctaata cacgaaatgt
    30661 attggtcgtt ccagtattac cgctcccgtg ttgtcggata tcttttttga aaaacaattt
    30721 gccaatccag acaccggaag gaatagaacc taaaagatat gctaaaataa gtaataagat
    30781 caatttcatt ttttaccctc tttcaagttg aaaactatcc tttattttag catgaatagg
    30841 taacaggaac aattgaaaaa atgaaagata caagtataat agatggaata aaaagaaaag
    30901 caaggggtgg cagaaatgtc tgtaaaaaat ccaacacaag aaacgatttt caactatttg
    30961 gaagaagcaa aaaatattgc cgtcgtaggc ttgagcaata aaccagaacg aacaagttac
    31021 atggttgcac aggtattgca agacaatgga tataaaatca taccagtaaa tccattattg
    31081 gctggtgaag aaattctagg cgagaaagtc tatgcgaatt tgacagaaat cgatgatcca
    31141 atcgatatcg tagatgtatt tagacggagt gaatttttac ctgaaatagc aggtgatttt
    31201 ttgaaaactg atgctaaagt gttctgggca caattaggga ttgaaaacaa agaagcagct
    31261 gagatgctaa gaaaagcagg acgtacagat atcgtaatgg atcgttgtat taagattgaa
    31321 cttggtaagt tgaaagaaaa ccaaaaataa aaatgaccac aaagaagaga tgatcgatag
    31381 aggggaatgt cgaaaaagcg gttaggagat aattaaaatt gatttctcgt ctaccctctt
    31441 ttttttcacg accttttcta gtatgataat cgtggtgcgg ttttgccgca aagtattatt
    31501 gaaggagcgt ttgccttggc aaaaaaagta aacaacgaat acaatgactc gtccatccaa
    31561 gtattggaag gattagaggc agttagaaaa agaccaggga tgtacattgg atccactgac
    31621 agccgaggct tgcatcattt agtctatgaa atcgtcgata atgcagttga cgaagcatta
    31681 tcaggttatg gcagtgagat tgatgtgacc atccatgaag acaatagtat aactgttgca
    31741 gatagcggac gtgggatgcc tgtaggaatg catgcttccg gaataccaac agttgaagtg
    31801 atttttactg tgctgcatgc tggcggaaag tttggtcaag gagggtacaa aacttctggt
    31861 ggactgcacg gtgtaggagc cagtgtggta aatgccttat cgaaatggct aattgtaacg
    31921 atcgttagag atggcgtaga gtatcagcag aaatttaaaa acggcggtaa acctgacggt
    31981 actttgaaaa agataggaaa aacaaaaaaa gcaaacggga cgactgtcca ttttttacca
    32041 gatgatacaa tattcagtac aacgaaattt tcatatgaaa ttttagccga acggttaaga
    32101 gaatctgcct ttcttctaaa aggcgttaaa atctctcttt ctgatcttcg tggagaagaa
    32161 ccagtcaagg aaatttttca ttacgaagaa ggaatcaaag aatttgttga ttatctgaat
    32221 gaagaaaaag atacattgac accagttgtt tatttttcag gagaaaaaga aggtatcgaa
    32281 gtagaggtcg cttatcaata caatgacggt tattcagaaa atgttttatc ttttgtcaat
    32341 aacgttcgga caaaagatgg gggaacccat gaagctggta tgaaagcggc gatgacgaaa
    32401 tcttataatg aatatgcgag aaaagttggt ttgctgaaag aacgggacaa gaatctggaa
    32461 ggtagtgatt tcagagaagg gttagctgct gtactttcta tccgtgtacc tgaaaatctt
    32521 ctgcaatttg aagggcaaac aaaagaaaaa cttggtacgc cagttgctcg taccgtagta
    32581 gataacgtca tcagtgaaca aatgggcttt tatttgcagg aaaatagcga aatgagccag
    32641 atgttggtcc gtaaagcaat caaagccaga gaagcgagag aagcagcacg taaagccaga
    32701 gaagaaagcc gaaatgggaa gaagcgtaaa aaaggcgaat ctcttctttc aggaaaattg
    32761 acacccgcac aatcccgaaa ccctaagaaa aatgaattgt atttagtcga aggggattct
    32821 gctggcggat ctgctaaaca aggaagagac agaaagttcc aagcaattct acctttacga
    32881 ggaaaagtca tcaataccga aaaagctaaa atgcaagata tcttgaaaaa tgaagaaatc
    32941 aatacgatga tctatacgat tggtgcagga gtcggtcctg agttttctat cgaagactgt
    33001 aattacgaca aagtaatcat catgaccgat gcggatacag acggcgccca tattcaagta
    33061 ttactgctta ctttctttta tcgttatatg aaaccgctga ttgaagctgg gaaagtatat
    33121 atcgcgttac cgcctttata taaagtatcc aaaggacaag gaaagaagca agtcattgaa
    33181 tatgcgtgga cagatgacga attggcagca atgattaaaa aagtcggcaa aggatatatg
    33241 ctgcagcgat ataaaggact tggagagatg aatgcggaac aattgtggga aacaacgatg
    33301 gatccgactt cccgtacgtt gatccgcgtc cgtatcgatg atgctgcgca agctgaacgt
    33361 cgagtaacca cactgatggg tgacaaagta gaacctcgaa gaaaatggat cgaaaatcac
    33421 gtgcaattta cgctcgaaga agatggcagt atcttagata aaaaagagga tactgaaatc
    33481 tctccttcag tttctaatga tttattagat gaagaacgag cagataaaaa tgaaaacaat
    33541 cagttatttg aagtagaata ggaggtcacc tgatggaaaa tcgacaagaa gtacaagaat
    33601 taactttaga agaagtgatg ggtgaccgct tcggacgtta ttccaaatat attatccaag
    33661 aacgtgcact gcctgatatc cgtgatggat tgaagccagt gcaacgccgg atattatttg
    33721 ccatgaacaa agatggcaat acgtatgaca aaggattccg taaatcagca aaatctgtcg
    33781 gaaatatcat ggggaattat catcctcatg gtgacatcag tatttatgaa gcgatggtcc
    33841 gcatgagcca agactggaag ttgcgggaag tactgatcga aatgcacggg aataacggga
    33901 gtatggacgg tgatccacct gctgcgatgc gttatactga agccagatta tccaagttaa
    33961 gtggagagat gctagcagat atcgagaaag aaacagtcga tctcgtgtgg aattttgatg
    34021 atacagaaaa agaaccaaca gtccttccag ctcgataccc aaaccttctc gtcaatggat
    34081 cgacaggtat atctgctgga tatgcaacag aaatcccgac tcataactta gctgaagtga
    34141 tcgatggcac aatctatatg atcgatcatc cacaagcgag ccttgaaaaa ttgatggaat
    34201 atattcctgg acctgacttt ccaacaggag ggatcttgca aggaaaagat gaaatcaaaa
    34261 aagcttatga aacaggacga ggaaaagtta ttcttcgttc gaaaacaaag atagaggcaa
    34321 tgaagggtaa taaacagcaa atcgtgatca cagaaatccc atatgaagta aataaagcga
    34381 ccttggtgaa aaaaatggat gagatccgtt tgaataagaa aatcgatgga atagctgaag
    34441 tacgagatga aagtgataga acaggtctgc aaattgtcgt cgaattgaaa aaagacgcca
    34501 atgctcaagg aattttgaat tatttgttca aaaacactga gctacagatc aattacaatt
    34561 tcaatatggt cgctatcgac catatgactc cgcatcaagt tggactgaaa gacattttaa
    34621 gaagttatat cgagcatcga aagcaagtca taacaaaacg tagtcaattc gatttagcca
    34681 aagctcaaaa aagacagcat atcgtagaag gattgatgaa agctttatcg attctggatg
    34741 aagtaatcgc aacgattcgt gaaagtaaag acaaaaaaga cgccaaaaag aatttagtcg
    34801 acgtctttca atttacagaa gaacaagcag aagcaattgt cacattgcaa ttataccgtt
    34861 tgacgaacac ggatatcaca gagcttcaaa aggaatccga aagcttgatt gcgcaaatca
    34921 cagagttgaa taaaatcctt tcaaatgaca aagagctttt ctctgttatg aaaaaagaat
    34981 tgcgagaagt gaaaaaaaat tattcttccg ctcgtctaac gacaatcgaa gacgaaatcg
    35041 aagaaatcaa aattgatacg caagtcttag tggcacaaga agacgtaatt gtcagtgtga
    35101 cgcgagaagg ctatgtgaag cgaacaagcc ttcgttctta tagtgcttcc aaaccagaag
    35161 aaatcgggat gcgagaaggc gactacttgc tatactcagg tgaattgagt acattggacc
    35221 atgttcttct gatcacaaat aaagcaaatg tgatctaccg ccctgtccat gaactacccg
    35281 atttgaaatg gaaagatgct ggtgagcaca tttctcaaac aatctccaat ctgtctgtag
    35341 acgaatcgat tttagctgtt ttcccatatc aaaaaattca agcagagaaa acatttgtat
    35401 tcatcagcaa gaatggattg atcaagcaaa cacgaatgac agagttcgaa ccatggcgca
    35461 cctataagag ccgtccgctc agctgtatga agctaaaggc ggaagaagat gaactgattg
    35521 ctgtgtatct gaaagaagat cagccaggct tagatgtttt cttagtaacg catcaaggaa
    35581 tgggtttgcg ctatccttta acagatgttc ccgttgttgg agcaaaggca gctggagtga
    35641 agtctatgaa tttgaaagat ggagatagga ttatcaacgg gctgcttgtg ttagcagaag
    35701 gtgatacgcc gatcgtaatc gttactcagc gaggagctgt taaacgcatg cttgctcaag
    35761 aaatcagcca aacttcacgt gcgaagcggg gagtgacggt catgcgtgag ctgaaaaaaa
    35821 atccgcaccg tattatctat atgtcggaag gaagatcaaa agaaataacc ttgatgaatc
    35881 aaaaagggca gcagttaacg attgacccaa ctgatttccc tataggtgat cgaacatcga
    35941 atggatcatt tgcgatggat gagaaaaaag gtggagaagt catcgaagtt atcgatgctc
    36001 ctgagatttt catcgagtcg taactgttgt agtattttaa tgaagagacg ccatctgtat
    36061 taatacaggt ggcgtctctt tttcattcat ttttaataaa tccttatacg tttgcaaaaa
    36121 aagatgaaag tgttcacaat gcttaaatgt tggtatgtca acgttgattt aaatatatgt
    36181 aaataaaaca taaaaattgt taaataaaaa acaagaaaac ccttgcaaag atagggtgct
    36241 gtgttatact aatcacgtaa aaactgttac ataaaacaga aatttaggag gtccatacaa
    36301 tggagcaatg gaatggattt aaaggtacca aatggaaaga aggcgtagac actagagact
    36361 ttattcaaaa taactacact gaatatcgcg gtgatgacag ctttttggaa ggcccaacag
    36421 aagcaacaga aaaattatgg gatgaattgc aaaagttaaa tgatatccaa ttcgagcgta
    36481 acggtgtcta tgatatggac acagaaatcg tatcaactat tacttcacac gatgcaggct
    36541 atttggataa agatttggaa aaaatcgtcg gtctacagac agacaaacct ttgaaacaag
    36601 cattcatgcc gttcggcgga atcaatatgg ctaacaatgc gctaaaaagc aatggctata
    36661 caccaaacga tgaattgaca catattttca ctgactggcg caaaacacat aatcaaggtg
    36721 tatttgacgc ttatactgct gaaatgcgtg ctgctcgtaa aaacaaaatc attacagggc
    36781 ttccagacgc ttacggtcgc ggacgtatca tcggtgacta tcgtcgtgtg gcattgtatg
    36841 gaatcgacta tttgatggaa caaaagaaaa aagatcatga tatgactggt tatcatacaa
    36901 tgagtgaaga agtgattcgt ttacgtgaag aaatcactga acaatatcgt gcattagcgc
    36961 aaatgaaaga aatggctgcg aaatatggct atgatatttc taaaccagct gcaaatgcaa
    37021 aagaagctgt ccaatggcta tacttcggat atttagcagc aatcaaatca caaaatggtg
    37081 ctgcgatgtc aatcggacgt atctcagcat tccttgatat ctatatccaa cgtgatttgg
    37141 ataatggagt aatcacagaa aaagaagcac aagaattgat cgaccactta gtaatgaaat
    37201 tacgtatggt caaattcgcg cgtacacctg aatataacca attattctca ggattcccta
    37261 tctgggctac tttatccatt gcaggtatgg gattagatgg tcgttcatta gttacaaaga
    37321 atgatttccg tctgcttcat acattaacaa atatgggacc ttcaccagaa ccaaacttga
    37381 ctgttttgta ttctgaacac cttccagaag gattcagaac atatgctagc agaatcgcta
    37441 tcgaaagttc ttcgattcaa ttcgaaaatg ataatcagct tcgtgaacaa tggggttctg
    37501 atgattgtgc gattgcttgc tgtgtatcag gaacagttgt cggaaaagac atgcaattct
    37561 ttggcgctcg tgctaacttg gctaaaacac tattatatgc catcaatggc ggagtcgatg
    37621 aagtgactgg cgcccaagtt ggaccagaat atcgtccaat cacatcagaa atactagact
    37681 ttgaagaagt gaaacataac ttcatgaata tgatggattg gttagcagaa ctatatgtca
    37741 atacgttgaa catcattcat tatatgcatg ataaatattc ttatgaagca gctcagctgg
    37801 ctttgatgga ttctgacttg caacgcacat ttgctacagg tattgctggg atctctcatg
    37861 cggcagacag cttgtcagcg atcaaatatg caaaagtaaa agcaatccgt aatgaaaaag
    37921 gaatagcagt tgattttgaa gtggatggcg acttccctaa atacggaaat gatgatgacc
    37981 gtgcagatga attagtttca tggatcttgg aatacttcat gggacaaatc aaacgtcata
    38041 aaacttaccg caactcaaaa ccaacaactt cattattgac gattacttca aatgttgttt
    38101 acggtaaaaa tacaggaaat actcctgatg gccgtgaagc tggaaaacca cttgcgccag
    38161 gagcaaatcc aagttacaat gcggaacaaa gtgggttatt agctagcctg aactcaacag
    38221 ctaaaatgaa ttacggctgt gcattggatg gaatttcaaa tacccaaaca atcaatcctt
    38281 caggtcttgg aaaagaacaa gatacaagaa tcgaaaactt gcgtaatgtc ttagatggat
    38341 acttctcaaa aggagcttat catttgaatg tcaatgtatt cagtaatgat ttattgcgtg
    38401 atgcaatgga aaatccaatg aaatatccga acttgacgat ccgggtatct ggttatgcag
    38461 ttaaattccg tgacttgaca agagaacaac aattagatgt cttgatgaga acttcacacg
    38521 acagaatgta aaaagttcgt gaaaaaagtt tttcgactta gcgtcgaaag gacgttgaat
    38581 agccatcgca tcaaaatcga ataatcagta tgcttatttg aaagaaacga tttacagggg
    38641 caggacttgt atgttccgtc cctgtaaatt ttacacataa cgaagcggca attgcgaaaa
    38701 attttgttca gaaagcttcg cttcgacagc taacttgtac ttaaaatgaa atcaaagaaa
    38761 agatgtgaac ttaaatggaa gaaaaaacaa tcggctatgt tcattctatc gaaactttcg
    38821 gttcagtaga cggacctgga ctgcgctttg tcgtgtttat gcaaggatgc cgcatgcgtt
    38881 gccaattttg tcacaatcca gatacttgga atattggcgg agggaaagaa tacacagcag
    38941 atgaactttt agataaagca gaacgcttca gaccttactg gggagataaa ggtggtatta
    39001 ctgttagtgg tggagaacct ctattgcaga tcgatttttt gatcgaacta tttaaaaaag
    39061 caaaagagcg gaagatgcat actacattgg atacatgcgg aaaacctttt acgtatgaag
    39121 aacctttctt ttcacgtttt caagaattga tgaagtatac agatttactg ttatttgaca
    39181 tcaaacacat cgataatgaa gagcataaaa aactaacgca ttgggacaat gaaaatattt
    39241 tagaaatggc tcaatatttg tcaaaaatca acaaaccggt ttggattcgc catgtactag
    39301 ttcctgagcg aagtgattat gatgaatatt tgatccgact agacaacttt attcaaacct
    39361 tgtcaaatgt agacagagta gagattcttc cttatcatac gatgggaaaa tacaagtggg
    39421 aaacacttgg tttgaaatat ccattagaag ggatcgaacc acctacaaaa gaacgtgtcg
    39481 aaaatgctaa acgattgctt catacagaag agtatacaaa atatttaacg cgataggtcg
    39541 catcggaaag aagataagta ttgcttcttt ccgttttttt gctattatta aataggagaa
    39601 atcacaaagg agcgaatgac atgtcaaaaa ttttagtttt tggtcaccaa aatccagata
    39661 cagatgcaat cggggcagcc attgcgtttt cacacttgca aaatgaatta ggtaaagaaa
    39721 cagaagcagt tgctctaggt acacctaatg aagaaacaca atacgcatta gaccactttc
    39781 agctatctgc accacgagta gtagagtcag ctaaagcaga aaatgcagat gtcatgcttg
    39841 tggatcataa tgaattccaa caaagtattt cagatatcag cgaagtaaat attttagctg
    39901 tagtagacca ccatcgtatt gcaaactttg aaacagctaa tccattgtac tatcgtgcag
    39961 aaccagttgg atgtacaagt acgattattt taaaactata taaagaaaac caagtaacaa
    40021 ttcctaaaga aatcgcagga atcatgctct cagctatcat ttcagataca ttgttgttca
    40081 aatcaccaac atgtacgcct gaagatgaag cagctgcaaa agaacttgca gcaatcgctg
    40141 aagtcgatct aaacacttat ggacttgaaa tgttgaaagc aggaacaaat ctaagtgata
    40201 aatcagctgc ggttcttctt gatttggatg caaaaagttt cccaatgggc agttccaacg
    40261 ttcgtatcgc tcaagtcaat acagtggacc ttaatgaagt gatggatcga caagcagagc
    40321 tagaagcagc aatggaagat gaaatgaaag caaatggcta cgatctgttc gtattagttg
    40381 tcacaaacat cttagacagt gattcagaat tacttgttgc tggtaaagga aaagaaaaag
    40441 tagaagaagc tttccaaaca actttgacca acaaccgtgc tttgcttaaa ggtgtcgttt
    40501 cacgtaaaaa acaagtcgta cctcaattga cggctgtatt tagctaatca aatggtagta
    40561 tgaagcattc aaaatgaatg gataagcgat tgatataaaa agaaacatag aacatactaa
    40621 aggccgaaga aatggaggag aataacagtc aattatgact cttgttcttc ctccttttta
    40681 cgtaaaagcc ttttaagtaa aataaaacgg atagaaaaga gaacatatat gaactattat
    40741 tcaaaaaata aggaaaactt ttatcaaaaa ctgaccgatg atccgctttt ttcttcattg
    40801 accgattatc tatatgaaca tcgagaacaa gaaacgattc taagagaatt gaaaaaagaa
    40861 ttttcgcaaa ataaattttc ccattttctt gatttattga tcgatgcagg attaatcaag
    40921 agggaagaac gaagatatca tttgaacttt ccaatatttg atcctaaaga ttacttgcaa
    40981 caagccactt cggctgctga aaccatagct gaacaattga agagactctc tgttgatgaa
    41041 caaaaattag cgatgggaga agttatttgg gcgtattgtt tcgaagatga acggaaagaa
    41101 gcgtattttt atggagtaag aaacagccga gagacagaat tgctgaggac aactgcagga
    41161 aatgagaagt accgttttat cacactttcc tctatagaac acttcccgct tactttggct
    41221 aattactttt ttgtacagaa gaatcagctt cctgtaacga aggcattcaa agaattagcc
    41281 gaacttattg gagacgtaaa tgaatcgtac ttttttgatc aaatagaagt tatcgttgac
    41341 aggatacgta agaataaata taaaaatcgc cggcctagta ttttccatca gtctctactt
    41401 gtgacgaata ctataaagga agaagaatca ttcacattag aattacctat tgtcgaaaaa
    41461 aacaattttg agattgaatt accaacattg gatccttcat tgacaatgga ggaaatagcc
    41521 tttcttaaac gacaaatctt ttctgagctg agcaagaaat ttataccgca tgcattttct
    41581 tatatcaaag aatatggaac agttttagtg agtaaaacct aagagagcag taagcaaaaa
    41641 ttatactgac tcttgcagga ggagcctttc tcttccttta tggttaaaag aattatttga
    41701 gtattttgcc tatctaccac ctattagatt cactacatgg tctggaaaca aaggaatgca
    41761 aaaaaatggt gagaaatgtg gaatgacaaa ggaagtagtg ataagaaaag ttagattttt
    41821 aaataatcaa tattatgatt cagtcaagta tggcatttta tgggaagaac tagctgatta
    41881 gattaggaag tccatttgat aatccagaat cactaagaaa cgaatggcga gtcattaata
    41941 atttatccaa tcttgggtta ttcttttaga ttgaagtgtt taaaactaaa aaagcattta
    42001 tgaagtcgta atagtcaggc ttcaaaatgc tttttatttt gtttgttttt tctttgccac
    42061 ataaatacac ctagactgac gatgaagagc atatccaaca gatttcctgc gacttggatc
    42121 gtcgaagggt tggtcagtgg cattaaagca aggacaaaaa aggaaaacaa gctgtaaata
    42181 aaacaggtga caggaactaa tcgaaagaag agcggacatt tcaattggct aaaaatgagt
    42241 gaaaaaataa aataagtaaa aatggataaa atcacgacta aaccattcat gataaagaag
    42301 aaaagcgtat tgtgattaaa cgaataaaaa agaattgcta aaaaagaaaa cgttttaaat
    42361 gcaatcaata cgcttgaagc ttcgtttaac gtaccaggat gtcgatgaaa aaaataggca
    42421 ccgaaataaa cagaaaacag taagccgcaa agagaagaca aaatcaaaaa aaacggattt
    42481 tcttcggctt ctgtgatatc agaatgatcc aaaagcaacc agcctaatgc actagaaggc
    42541 gcatgtgagg tcttaaggta tgaataacat agaaaaaata agatcgtcaa agttgcaaaa
    42601 aaagagccta aatgtaaaaa attagtggtt ctacgttgaa tttccataat atctccaaat
    42661 taaattgata agtatat
//
LOCUS       DAYYHX010000004        41965 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000004 DAYYHX010000000
VERSION     DAYYHX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.004"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1824
                     /locus_tag="ACHA6Y_000137"
     CDS             <1..1824
                     /locus_tag="ACHA6Y_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986265.1"
                     /translation="YNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMK
                     EIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQW
                     ITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKV
                     YLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFA
                     LALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCT
                     FQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTP
                     WIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQV
                     DFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILE
                     EAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIH
                     ALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQE
                     RFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            complement(1869..2873)
                     /locus_tag="ACHA6Y_000138"
     CDS             complement(1869..2873)
                     /locus_tag="ACHA6Y_000138"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM8986266.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(2884..3624)
                     /locus_tag="ACHA6Y_000139"
     CDS             complement(2884..3624)
                     /locus_tag="ACHA6Y_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8986267.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            complement(3694..4497)
                     /locus_tag="ACHA6Y_000140"
     CDS             complement(3694..4497)
                     /locus_tag="ACHA6Y_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8986268.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            complement(4497..5579)
                     /locus_tag="ACHA6Y_000141"
     CDS             complement(4497..5579)
                     /locus_tag="ACHA6Y_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM8986269.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            complement(5720..6988)
                     /gene="dltD"
                     /locus_tag="ACHA6Y_000142"
     CDS             complement(5720..6988)
                     /gene="dltD"
                     /locus_tag="ACHA6Y_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM8986270.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            complement(6991..7224)
                     /gene="dltC"
                     /locus_tag="ACHA6Y_000143"
     CDS             complement(6991..7224)
                     /gene="dltC"
                     /locus_tag="ACHA6Y_000143"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM8986271.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            complement(7269..8474)
                     /gene="dltB"
                     /locus_tag="ACHA6Y_000144"
     CDS             complement(7269..8474)
                     /gene="dltB"
                     /locus_tag="ACHA6Y_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM8986272.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            complement(8471..9985)
                     /gene="dltA"
                     /locus_tag="ACHA6Y_000145"
     CDS             complement(8471..9985)
                     /gene="dltA"
                     /locus_tag="ACHA6Y_000145"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM8986273.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            complement(9996..10145)
                     /locus_tag="ACHA6Y_000146"
     CDS             complement(9996..10145)
                     /locus_tag="ACHA6Y_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM8986274.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            complement(10426..12468)
                     /locus_tag="ACHA6Y_000147"
     CDS             complement(10426..12468)
                     /locus_tag="ACHA6Y_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8986275.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLTILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            complement(12458..13225)
                     /locus_tag="ACHA6Y_000148"
     CDS             complement(12458..13225)
                     /locus_tag="ACHA6Y_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8986276.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            13551..15740
                     /gene="nrdD"
                     /locus_tag="ACHA6Y_000149"
     CDS             13551..15740
                     /gene="nrdD"
                     /locus_tag="ACHA6Y_000149"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM8986277.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            16055..16657
                     /gene="nrdG"
                     /locus_tag="ACHA6Y_000150"
     CDS             16055..16657
                     /gene="nrdG"
                     /locus_tag="ACHA6Y_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM8986278.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(16711..17832)
                     /gene="dinB"
                     /locus_tag="ACHA6Y_000151"
     CDS             complement(16711..17832)
                     /gene="dinB"
                     /locus_tag="ACHA6Y_000151"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM8986279.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(17941..18813)
                     /gene="rsmI"
                     /locus_tag="ACHA6Y_000152"
     CDS             complement(17941..18813)
                     /gene="rsmI"
                     /locus_tag="ACHA6Y_000152"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM8986280.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            complement(18882..19229)
                     /locus_tag="ACHA6Y_000153"
     CDS             complement(18882..19229)
                     /locus_tag="ACHA6Y_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM8986281.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            complement(19222..20046)
                     /locus_tag="ACHA6Y_000154"
     CDS             complement(19222..20046)
                     /locus_tag="ACHA6Y_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM8986282.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            complement(20082..21020)
                     /gene="holB"
                     /locus_tag="ACHA6Y_000155"
     CDS             complement(20082..21020)
                     /gene="holB"
                     /locus_tag="ACHA6Y_000155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM8986283.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            complement(21034..21363)
                     /locus_tag="ACHA6Y_000156"
     CDS             complement(21034..21363)
                     /locus_tag="ACHA6Y_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM8986284.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            complement(21378..22022)
                     /gene="tmk"
                     /locus_tag="ACHA6Y_000157"
     CDS             complement(21378..22022)
                     /gene="tmk"
                     /locus_tag="ACHA6Y_000157"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM8986285.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            complement(22086..22682)
                     /gene="recR"
                     /locus_tag="ACHA6Y_000158"
     CDS             complement(22086..22682)
                     /gene="recR"
                     /locus_tag="ACHA6Y_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM8986286.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            complement(22707..23021)
                     /locus_tag="ACHA6Y_000159"
     CDS             complement(22707..23021)
                     /locus_tag="ACHA6Y_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM8986287.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            complement(23041..24786)
                     /gene="dnaX"
                     /locus_tag="ACHA6Y_000160"
     CDS             complement(23041..24786)
                     /gene="dnaX"
                     /locus_tag="ACHA6Y_000160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM8986288.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            25001..26248
                     /locus_tag="ACHA6Y_000161"
     CDS             25001..26248
                     /locus_tag="ACHA6Y_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8986289.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            26273..27457
                     /locus_tag="ACHA6Y_000162"
     CDS             26273..27457
                     /locus_tag="ACHA6Y_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM8986290.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            27454..28251
                     /locus_tag="ACHA6Y_000163"
     CDS             27454..28251
                     /locus_tag="ACHA6Y_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM8986291.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(28693..29349)
                     /locus_tag="ACHA6Y_000164"
     CDS             complement(28693..29349)
                     /locus_tag="ACHA6Y_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8986292.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            29542..29820
                     /locus_tag="ACHA6Y_000165"
     CDS             29542..29820
                     /locus_tag="ACHA6Y_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986293.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            29839..31047
                     /locus_tag="ACHA6Y_000166"
     CDS             29839..31047
                     /locus_tag="ACHA6Y_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8986294.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(31195..31560)
                     /locus_tag="ACHA6Y_000167"
     CDS             complement(31195..31560)
                     /locus_tag="ACHA6Y_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM8986295.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(31707..32480)
                     /locus_tag="ACHA6Y_000168"
     CDS             complement(31707..32480)
                     /locus_tag="ACHA6Y_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8986296.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(32654..33514)
                     /locus_tag="ACHA6Y_000169"
     CDS             complement(32654..33514)
                     /locus_tag="ACHA6Y_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8986297.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(33514..34932)
                     /locus_tag="ACHA6Y_000170"
     CDS             complement(33514..34932)
                     /locus_tag="ACHA6Y_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8986298.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(34989..35438)
                     /locus_tag="ACHA6Y_000171"
     CDS             complement(34989..35438)
                     /locus_tag="ACHA6Y_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8986299.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(35883..36146)
                     /locus_tag="ACHA6Y_000172"
     CDS             complement(35883..36146)
                     /locus_tag="ACHA6Y_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM8986300.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(36245..37540)
                     /gene="rho"
                     /locus_tag="ACHA6Y_000173"
     CDS             complement(36245..37540)
                     /gene="rho"
                     /locus_tag="ACHA6Y_000173"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM8986301.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(37537..38808)
                     /locus_tag="ACHA6Y_000174"
     CDS             complement(37537..38808)
                     /locus_tag="ACHA6Y_000174"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8986302.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(38974..39843)
                     /locus_tag="ACHA6Y_000175"
     CDS             complement(38974..39843)
                     /locus_tag="ACHA6Y_000175"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM8986303.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            39946..40158
                     /locus_tag="ACHA6Y_000176"
     CDS             39946..40158
                     /locus_tag="ACHA6Y_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986304.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            complement(40135..41745)
                     /locus_tag="ACHA6Y_000177"
     CDS             complement(40135..41745)
                     /locus_tag="ACHA6Y_000177"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM8986305.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT        12392 a         8719 c         7364 g        13490 t
ORIGIN      
        1 tataataaaa aagaatcttt ttatttacgt ataaagtgga gcataatcat tatattatca
       61 ttgttatttt taacaggaat tgcttatttc ttttatttta ttttttctcc tgcggacagc
      121 aatcaaatga aagaaataaa agattcgtgg gttttctata caaaagatga tcctgatttt
      181 aagtttgaca gcaagtacac tcgtacgatt cctaccgtgg ataaagacga aacattcatt
      241 atggaaacga cacttgaaaa accgttggac gaagcaaatt tactaatcaa aggaaaccat
      301 caatggatta ctgtatatct aggcgaaaag attttatacc aacggaaaga ttatcaggaa
      361 gaaagcaatc ccggactctc attggctgtg atagatttac cagcaaatta tatcggaaaa
      421 aaattgaccc ttacagtctc atctccttat cagaactatg ctggactgcc accgaaagtt
      481 tatctaggaa caactggacc attgattagt tttatttatt cacaatcagt gccgcaagta
      541 ttgacgatga taatcgctgt ttttatcgct atagggacct ttttctatac gatctacttg
      601 atgtataaac aaaaacgtct tgaatactca cttattctct taagttgctt tgctttggca
      661 ttaggatttg aagctgtatc agaagatatt ttaagtggta ttctttttga accattcgtt
      721 cattcttttt tatctcattt atttattatc ttaacgtcag ctttcatcat ctgctactat
      781 tggagccgaa tgttttacta taaaaaatgg tacggaattt tttgtacgtt ccaacttttg
      841 atttttatcg gcgttctgct ttatgcaact tttactcctg cagaacttcc tgaattaatg
      901 ccgattgcta atattgctag tgttataagc actttagtca cttcacttgc atgcattggc
      961 gaagcatata agaataaccg tttttatgta gcttgtacac cttggatcgt cttggtagcc
     1021 attatccatt gcttgattta catccaaacg gcattaggta tctatcaaac aacgatcaat
     1081 tggtctacga ttttatatat tatcattcta atcgttatta tcagctacaa tctaatcgat
     1141 cacatcttaa ataccgacaa agatcggaga caagtagatt tcttaaaaat caaaaatgat
     1201 ttgttggagc aacattatga gcagcttcga cagcatatcc aagaagtaaa tacacttcgc
     1261 ctggaatttg tccaagagat ggagaatctg caagatttaa tgcatacgga ccaaacaaaa
     1321 gctaaaaact atgtggaaaa gatattagaa gaagcaaaaa atttcgaaat gctgtgttct
     1381 ttttgcaacc accaggtgac aaatcttatc ttagcgcgtt accagaagtt agctgccaaa
     1441 cggaatattc agataacctt ccaagcagat cttcccgaag aacttcctat caacgatgac
     1501 gatctggctc aacttttgat ccacgcttta gagcattcct ttagagaaac tcatgccatc
     1561 gaaaatccac tgtatcgaaa aatttatttg tcgatacacg aacaagagga acatttcgtc
     1621 atttgttgcg aacatagtgc acattacgat acaaacctat ttagtcgagg catcacagaa
     1681 gaattagatg atcaggaacg ttttgactta atgatgatga aagacgtagc ggatcgttac
     1741 acaggaacac taacgcagga aaaagatact ttcattgatc ggatcactgt tcaactctct
     1801 cgaaattacc cgaatcctgt ataaaaaata gcggaatcca tatatgtgga ttccgctatt
     1861 ttttattttc aataaagtaa ggtaagcaat tcttctggtt ctatcgcacc aaaatatttc
     1921 ttcacatcaa tttggctgac tatttttgat agagatgctt tatcatattt ctgtccaacc
     1981 aatttttttt ccacgtcaga gatatctcct agtccgaaga aatcgccaaa gatttttgct
     2041 tctttgatca ttccttctga gacattcaaa tggacttcaa tcgaaccgat agaaaaacgt
     2101 ttttggcgag agaaattgaa atctggagac ttcccgtaat tccaatccca atttcgataa
     2161 tattcctcag agattcgatt gatttttttc caatcttcat cagtcaattc gtacgttttt
     2221 acttcttcta ctgactctac gccgaaaatt ttcaacaaga tttcttgacg aaattcttct
     2281 gttgtcattt tttgcttttc ttctggaaga aatggtttga tattcgttac acgagaacgg
     2341 atcgatttga ttccttttga ttcgattttg tcttttttta cttttaatgc attgactact
     2401 tcattgatat cagagtcgaa cataattgtt ccatgagcaa acatccgacc gttggtagca
     2461 tacatcgcgt tgccagaaaa tttcatgcca tcaatgacta gatcatttcg gcctttcaat
     2521 tccgccccag caacacccaa ttcatgaagt gcttggatga ttggctgtgt tactttcgca
     2581 aagtcacgga atgaatcccc atcatcaggc atgataaaac taaagttcag gtttccaaaa
     2641 tcatggtaga cagctccgcc tccacttaag cgacggacaa catgaatgcc attttcttcc
     2701 acataatctc gattgatttc ttcaagcgta ttttggtttc tgccgatgat gattgaaggt
     2761 tcattgatat aaaagagcaa aataggttca tctaaaggca tttcttgcag caaatattgt
     2821 tcaattgcta aattgattct tgggtcgtgg ttttcatttg gtacaaaaat catgttcttc
     2881 ctcctaaatg ttaaacactt tgtcaactgc agcaagttct atatcaatcc ctttgcctgc
     2941 tgccttttgc gcttcatttt tcaattgttc taaatcacca aactgaggta ggtgtgtaag
     3001 tacaagtttc tttacgttag ctgatttcgc aagttctcca gcttcttttg aagtgaaatg
     3061 ggcatgatga tgttcatttc cttcaaaaag ataggtatct gctagaaaca aatcagcatc
     3121 tttcgcaaaa tcagcgaaac tttccaaata acctgaatca cctgtaaata cgaatacttt
     3181 ccccgttgtt tcttctacga tacgcatggc gtaacatact actggatgga ttgtttccat
     3241 aaaagtaatc agaaaaggac caatttttaa ttcttcagct tcaaaataag gatgtccttt
     3301 tgagacattt tccaacgtca attcttcaaa gtgaatggaa tcttttgtgt gtccataaat
     3361 tgaaagcaat gtctccggat ttctcttagg atgaagttgc cagtaatact gtaatacacc
     3421 aaggtcagca atatgatcat gatgataatg gctaaggatc acagcatcta gatcaagtgg
     3481 atcaaggtgt tcttgtaatt tgatcagcgt agcactacct gcatcgatca acaaattgaa
     3541 cccgtcagat tgaagcaaat aacctgttgt cccttgattt ttataaggat acgctcctaa
     3601 gcatcctaaa acagttaatt tcatcgtaca ttccccctca aagatttatc ctaccacaaa
     3661 agttattttt ttgatgaata aactgcttac atgctaagta aaataggcgt ctggacctcc
     3721 tttttcaatc cgtgcttgtt cataaaattc tgcaactgac acattcacat aaacaatgac
     3781 ccacaaaaga ccgatgaaca aagcgattgc tcctagcaaa taccagccga taaaagacag
     3841 ctgcaacaaa atatacgtac caattctatc tttcaataaa aaccagccat atttcaaaca
     3901 ttgaataacc gacaaatcag gatagtcgaa ctttgtccat gcagcaaatt ggaataaacc
     3961 gccaacaata ctcatgatga gtgctgaaac aaaaatcatc cctatcatcg ataaaacaaa
     4021 gctgatatca agaaaatctt ctggcatgac acccgtaggt accgcatttg aactgaaaag
     4081 cagctgcata taagtactca ctccgctgat taaaaacatg ggcagaaaga ttaaactgtt
     4141 gagtagccag ttgatgactg aattgatcaa attgatgcca agtatcgaac caaaatactt
     4201 tctttggaaa actgaaaata aaaggttact ttttggtctg ccaccccgta caactactag
     4261 cgcaatgtaa aacaatgcat agttgaatgc gaataaaagt aaattatcga ctagaaaact
     4321 caataagtgc tgctgcatgc tttcttccgg aaaaggactg aaaccgtcaa aaaagttctg
     4381 gatgacgctc gtaaatatta tagataagaa aacaagccaa gtattgattc cccattgatt
     4441 ttccaaagaa actcgtgctt ttttccgata ttcagaaata ggtattctat tattcattat
     4501 tcataactcc gaattttttc aactgtttca ctatcaagcc gtttcactaa ttctgtgact
     4561 agtttcactg tattcaaata atcatcttca tgaatcactg atgtatggga atgcagataa
     4621 cgaacaggaa cggtaatggc tacagacgga acaccatctc ttgtcaaatg catttgtcct
     4681 gcatcagttc ctccgccaga aatcactgta tattggaatg gaatatccat ttcctctgct
     4741 acatcaataa caaaattcaa taatttttta tgcgggatca tagatgcatc aaaaatcaaa
     4801 atctgtgggc cttttcctaa aatcgaatct gcttcttttg gtgtcattcc tggagtgtct
     4861 cctgctgtac cagtatctaa agcaaaagca atatcgggat ttacaaggtg tgtacttgtt
     4921 cgagcacctc gcaagcctac ttcttcttgc acatcgcttc ctgcgaataa aacatttggg
     4981 tgtgcttctt tagatagatt ctctaatact cgcagagaaa cagctgtccc gatacgatta
     5041 tcccatgctt ttgccagtaa atatttactg ccattcattc gtttgtattc aatatatgga
     5101 gtgaccatat ctccaggacg tatgccccac tctttggctt cttcttggct tgatgcgcca
     5161 atatcaatga acatatcctt tatttcataa ggtttatttc ttacttgagg tgttaaaaca
     5221 tgtggtggtt tggaaccaat cacaccgtgg acgattttcc cttcacgtgt tttgatttca
     5281 acttgctgag caagcatgac ttgcccccac caacctccaa gagtttggaa ttccaagaat
     5341 cctttttcgg ttatttttgt taccataaaa ccaacttcgt ccatatgtcc agaaataaag
     5401 accttggggc ctccgccagc accatgtttg gcaatcacac tgcctaaacc gtcataaaaa
     5461 atatcatcag cgaatggttt ggcatattct ctgaatactt ctcttacctc ttcttcattt
     5521 ccaggtactc cttttgcaga agttaaatca ataagtagct gttcttcttt tgtatccaaa
     5581 tgttttcccc catttctata ttcatattgt gattatacca ttgcttatga tttttgttca
     5641 ggcaaacttg gtatttttat cttttattca gaatattcaa aaataaagaa aacaagcgag
     5701 caacactcgc ctgttttctt tattttaatt gtagtttatc tggtatttga tgttgccaag
     5761 atttgctaaa aaaagcatcg tcaagatgat acttactagc agtaatatga ttttcttcta
     5821 aaaacggtct gatctgttga tctgcagcca accagccttt ccagcctaga tggatcgtgt
     5881 cttccataaa atagttggta cccgcttgat tgacaaaatc agctattcga ttgaatcctt
     5941 ggctatttaa ttgaaatttg atctttttgg caaatccttg taacatttct tgagatagtc
     6001 cggtataatc gctccatttc tcgttaaccg gtgggatgat gaacaatact tcgttatggt
     6061 tttttgctaa ttgatccaag accagctgaa aatcagaaaa ttctggcgaa aaacgataat
     6121 cccaattttt ttgtgactgt ttgagctctg gctcgtgggc tcggatacga tgcgtataaa
     6181 aatcgttttt caacccgaat ggattattcg tcgtttctcg ttctccaatt tgatttgcta
     6241 aaatggataa ttctgtttct tggtattgtt ttggtaatcg ttttgactca tgatcgattt
     6301 tttgctgtcg atctttcaac ccaatattgc tgaatacttc atcctctctc ttcagcatgt
     6361 tccattggct gcgcagctga ttcaaagatt caggtaaagg tagcttccct tttttgatcg
     6421 tttgcagaat cgccgtcaac gtatcattct cttttacttt tgagaaagtt aataatcttc
     6481 tagctaaata gcgatcagca ggcgttactt tcttcattga aaacaaccag tcgtatgttt
     6541 gaagttcaga ataatacgta ttaaagtaat cagtttttac accattttta acgaaccact
     6601 gaggagaaat aataaagacg actttcttgt ttttcatagc atctcctgca gaacgcatca
     6661 tcatatattg gcttaacgat tgtgtcccgg gtgctcccag caaaaatgga cgatagtttc
     6721 gctggtattt ttctgccaga acagacggat gaaatggact gattctgctt aattcacttg
     6781 agccaaaaaa aggaatatat tgtttttcag ataatgcata attttttatc gcatctcctt
     6841 tcaacacgtt cactgacatg gaagaagagg cacgtctaat cgtttctgga ttagagatat
     6901 ctacttgata ggaggaggta aaaaacacaa acagtaagcc aacagctaaa acaaccgggc
     6961 cgaaaatagc aaaaagtttc tttttcatac tcaacccttc aatgcagttg cttgttgaat
     7021 gattttattc ggtgtgcacc aatcctctcg ctcgaattca gatacaggga cttggatatc
     7081 cagctctccg tctagctcga ctaataattg aacagatgct aaagaatcaa gcaacccttc
     7141 atcaaataga tcaatatcac gattttttct tacttcatct gttcctgtta tttcttctaa
     7201 gattgataaa atttgttctt ccattttcct tttctccttt gttatataaa ttaatatgtc
     7261 actattcgtt agctaaaaaa aagtttatct aaaaatcctg aaaaaatcag gaagctaaaa
     7321 catactgctt gaaatgtcaa aaatacggct aagccatgcg taaatttgtt agaaggcaac
     7381 tgctctttat gcttcttctt aaatcggatc catgcatctg tcagacagat cagacaagca
     7441 tgatacagtc catatacgat gtagtaccac gtcaatccat gccatatacc cattagaaga
     7501 aacaaagcaa aataaccgat atttgacatt acgatcttgc ttttaaaaaa ttttttcttc
     7561 atcatgaaga acatcaatcg catatataca taatcacgga accaaaagga cagcgtcata
     7621 tgccaacgat tccagaattc tttgatattc catgataaaa aaggtttatt gaaattgatt
     7681 ggtgtctgat atcccatcat gtagcttgta ccgactgcaa ataagctata gccagcaaaa
     7741 tcaaaaaaca aatacatact gtacacgtac atataaccta ctaatgccca tgaaatacca
     7801 ccatgctgta aggcaatgcg tcccactaca ggaagcagta cttgaccaaa ataataaccg
     7861 ataatgaatt tatataaaaa accgacaaaa atattttgga cgccttttcc caaaagatca
     7921 acatatttat cacgaacagg cggatggagc tgatcttttt caaatctcct atagcgatcg
     7981 attggtccag aagagattgt tggaaagaac agcagaaact ggatataacg aaatggctta
     8041 tatgttttga tcatgccatc tcttatttcc atgatcattt gtacagattt aaaggttaaa
     8101 taagagattc ctaaaaaacc aagtaacgat gcttttccat gttcaaaaaa aggagtcact
     8161 ttcgttaaaa acagtggaat tatcgctaaa ataacggcgg caaaaaacca tcctgtctga
     8221 tttttccttt gccgataaac aaaatagccc catacaagta tcgtctgcca caaaacatac
     8281 ccaatcagcg ccgctccctg ttgccaataa ttacctccaa agctgacaaa taaaaagaaa
     8341 agtgtcactg ctgattgata aactggtaat cttttttccc atatcaataa agatagaacg
     8401 atcggtacaa gcgcaatggc taaaagaaaa aaataaaacg gctctgcata agggatcata
     8461 tgaggtaaca tcattcagga ttcacctcag ctgttaattt ttttcgatcg atttttccat
     8521 taactgtctg aggcagcaga tcgacataat ggaacttctg aggaatcata taatccatta
     8581 caacttctag taattgttct ttgatagatt tagtcaactg aaaatccggc tgtttcgttt
     8641 tgttttctaa tacgacaaaa gcaaccagct gctgtacttt tgttccttgg tattttggta
     8701 cgacgacagc ttgtttgatt cggttgtctt ggagcagata atgttcgata tccccgagtt
     8761 caatccggta tccatgtaat ttgacctgct gatccatacg tccttcataa aataaaagcc
     8821 catattcatc tagtctacct gcatcccctg tatggtaact tgctaagtca ttttgacaaa
     8881 agaacgcagc agctgtcttt tcgggattgt tcaggtaacc ttttgatacg cttggtcctg
     8941 caataatgat ttcacccgtt tctccttttg aaacaggttt accttcttgg acaatagaaa
     9001 ctttcgtgtc tttttttacg tagccaatcg gtagtcggtc ataactatct agtacatgct
     9061 ggtcaatctg aatacttgaa atagctacag ttgcttctgt tggaccgtaa gtattatata
     9121 tccgtgcatt tgggaatcga tcaagtagac tttctgcagt ttttttcgtc aattcttctc
     9181 cgcaaaataa aaacatgtcc aattccggca cattttctcc attgaatgta ggttccatca
     9241 agcaaatatc cataaatgat ggagtagaaa cccacacatt caacttcaag gctggcaatg
     9301 tcgcgaataa ttgacgaaag tcttgaacaa ctgctttatt taacggcttc agcattccgc
     9361 cagaaactaa tgcaggataa atagaaaata ccgataaatc aaaagaaaat ggcgcttggg
     9421 ctaagaaatg cataccaggc ttgatcccaa agtcttccat cgtccattga acaaagctga
     9481 ctagattatc atgactgatt tgtacgcctt ttggttctcc agtagtacca gaagtaaaaa
     9541 taatataaaa attttctgta ccttttacgc tatttctggg ataaaatcct actggtatct
     9601 tcttgatttc ttgaaattct ttttttgtta tgacaggaac cgaaggaata tcttctatcc
     9661 agtccccgat actgatgacc atagatggtt tagccacccg aaagatcgat aggatacgct
     9721 ccttgggtgt atgctcttca atcggcaaat aggcatggcc agatttgaca acccctagaa
     9781 atgagacaat catttcaaac tccaaattgc caaaaacgac aattggtcca tcgccttcta
     9841 cctttttttc taaataataa gcaagtgcgt ctgatgcttg tttcaattca ccaaaagtat
     9901 gcgtctcatc cgcctgataa gcaactcgat caggttcatt tattccccaa tcatcgattg
     9961 cttcaataat cgttttgatt tccatactct tcctcctaaa attcgttata gataaaagtt
    10021 cctccttgaa tatttttata gtgatacaga taaatcagga acagcagtac tgcactataa
    10081 aaaattgttc gaatgatgaa ttttccccaa taattcaatt ttggattaga acgccatttg
    10141 ttcattggat tccctcctca tataacctca cacaaagtgt aactaatttt gacaaaaaga
    10201 aacatgtgtc tagcttacag aaaccgtttt tttactaaca gttttgtcac atagctttta
    10261 taaaaatagc acatcaaatc actgcttttt tctttataat ttctcgtact tttaaaaaat
    10321 ataaaaaata aagaggttgt gacaaaaatc gtgtcacaac ctcttattcc aaataaatgg
    10381 tattcaaaat ttagttctat ttattatctt gtcttattcc gtatatcatt cctcgataat
    10441 ccgacaatat aactgactcg taatgacata aagcaaaaga tagataagga tcaataagcc
    10501 actcacaaga taagccaacc agtaatcggc agtttttata tactgagtga agacattgat
    10561 tgcaaatact gcgtgagtga tccctaatat catgggtgga aagaaggtaa gtgcgttttg
    10621 ctcgaagatc atccgtctga tccttctttg tggaatcccc agcttagtca ataggtgata
    10681 attttctttt tcttcctctg cttctgctaa ttgccggact gtcaaaatac taccagtagc
    10741 aataatcacg atcatcccga caaatagtgt gacaaaaata ttgataccga ttcgccgatt
    10801 aactgcacgc aatttcgaat agctggaaga gaaattcaaa cgagagcttt cttggctaaa
    10861 ttgtgcttcc ttcatttggt catccggaag tttctttgtg ctaatttctc cttgtagact
    10921 tccatttacg atatcataag aataataaac agcattgagc cactttatcg acaggtcatt
    10981 gctcagtcgt tcatctattt tttcattatt tccacctgat acatctacat tgactacttg
    11041 ataatccaca ccttttgctt tttcaaaaac ggtatcatct acaatcagta caggacttag
    11101 atataccatt gtttcgtcac ctaatacact tggaagcatt tgttgaatct tcaattcttg
    11161 attatctggc agtccaatac tagatccata taatgaaata ttccgaaaaa gagtttgcat
    11221 ttgatcaagc aaaacagtat gaccaggtga tttcaaagaa atgttcggca aatctggatt
    11281 aacttttctg aaggcttgat attgtgtttc cgtgatcaga ttagcagttt gaaacatagg
    11341 ttcttcgaga ttttgactat agatactccc aatgacttta taatgcaagg tgatttcttt
    11401 agttatcttc tgcccttcct ctgctagaat cggcttgatt tttttctctg tctcaacatc
    11461 aaactgatag tccatcgggg aggaaacttt ctcctggtag gataaaatag tagagatact
    11521 agccgtgccg ccaattgtca ttaaagaaat tccgagaatg actgtaatca gagaatttgt
    11581 acgccaactt ttgagcaaat gaacttgcgt gttacccatt atcaaaaaat tgataccata
    11641 gtaataaatc ttccatttac tcaatatggt caaaaataac cgaaatgtat aacagaaaaa
    11701 caaataagta ccgaccacac aaacgaaaaa aattaaaaaa ggaaaaaaga aaactaatgt
    11761 atcaagataa tgataactga tcatccatga agtgatttcc ctaaaatgac tagctccaaa
    11821 atatccgctc gttaaaagta gtaacccgat gactgcgagt aatcggtgcc ttgtcctgaa
    11881 gcgcattgtt gaactatctg cttgttcttt ttctccaaaa cttcgaatca ttggatagcg
    11941 ccatattttc catattgaat atacagatac tgctaaaagg atgatgaaaa aagcaaataa
    12001 agtatctact actgatggaa tggagataaa aaatggacta gtaagatcca tatccatcat
    12061 tcgaaccaaa atcattgaaa agagtttgga aaaaataata ccgagtaata tcccagaaat
    12121 acaggcgaac aatccaatta tcatgatttc taacacatat atacctgata tctgcagttt
    12181 attcatacca aaaagctgat agatgcctat ttccttttgc cttctattca agaaaaatcg
    12241 gttggcactg aatacgaaaa aaagcaaaac aacagtaatg ataaatccac caaaactcaa
    12301 tccaccatct atcgaagtat cttgacttgc tcttctgact aaagattgat cgtaggtcat
    12361 tgcgctgaag gaataataga tcattacgga taaagtcaaa ctgaaaaaat aaacaagata
    12421 gcctcgtgac tgccgaaaaa aacttttaac ggctagttta tacagcattc atttttcctc
    12481 ctccgccaat cgtcgcctgc atgtctatta ctttctcaaa gaactctctt cttgtccctc
    12541 ttctgacaac ttcagaaaaa atcacaccat ctttaataaa taaaattcta tcgcagtagc
    12601 ttgctgtata tggatcatgg gtgactaaca gtatcgttgc tttttctttt tgattcatgg
    12661 tcttcaagta gttcaacaat tctgtagccg acttcgagtc caaagagcca gttggttcat
    12721 ctgcaaaaat cacttgtggc tgaacaacaa gtgctctagc agctgcaatt cgttgtctct
    12781 gtccgacaga aagttcatcg ggataccgtt tgagcagttc ttctatattc aacaattgag
    12841 ctacatgtct tactctcttt tccatttcct ctttagtcat ccgttcaact gctaggggca
    12901 ataggatatt gtcttttgca tttagggtat cgatcaaatt gaactcttga aaaatgaatc
    12961 ctagtttttt tcgacgaaag tcactgagct cactgttttt catttttgtg atttcttttc
    13021 cttggatcga gacactgccc atcgtaggca aattgatcgt ggacaagata ttcatcagcg
    13081 ttgtctttcc tgcaccactt gggcccataa tcccaacaaa ctcgccttct tctactgtga
    13141 atgagatatt atctaaggct ttgacgggag aaagtttgct gccgtagctt ttacttagat
    13201 gtttcacttc gatcatcttt tccaattctt ctcctcctct ttcatttttc atttctattt
    13261 atttgcatag tagcatatct acgaataatt tgctcttatg tttttgtcac ttttacttgg
    13321 actgaataaa aaattttatt caattatttt attcagcaaa attaaatatg caaaaaacaa
    13381 atccaccagc tacaagtttt cacctttttt cacaataaaa tatacactac atatagtata
    13441 gaaatccaat tatacagcac attgaatact atatatagcc gatatttagt tgctccttcc
    13501 ccgattttgt tttatgatag acaagaacaa tttagcagga gttgaagata atgaaagtaa
    13561 attatgaaga aaaaactgtc gttactcagc agccagagac atcccagatg aaagtcatca
    13621 aacgcgatgg ccgccatgtt gatttcgatg aacaaaagat caatgaagca ctgatcaaag
    13681 cagaaaagaa aattcacggc tcattatcac cacttactta tgaaaaaatt caaatgattg
    13741 cagacttggt tgtccaagaa atcaaaagcc ggtttgctga aaatgtcaaa atctatgaga
    13801 ttcaaaacat cgtggaacat atattactag aaaaaaacga atatgaatta gcagaagagt
    13861 acatcaatta tcgtaccaaa cgagattttg ctcgcagtaa agcaacagat atcaactttt
    13921 cgattgaaaa gctaatcaac aaggaccaaa ctgtcgtgaa cgaaaacgca aataaagaca
    13981 gtaatgtttt caatacacag cgagatctga cagccgggat cgttggaaaa tcaatcggac
    14041 tgaaaatgtt gccaccacat atagcaaatg cgcatcaaaa aggcgatatc cattaccatg
    14101 atttggatta tcatccttat acaccgatga cgaattgctg cttgattgat ttcaaaggca
    14161 tgctgaacaa tgggttcaaa atcggcaatg ccgaagtcga atcgccaaaa tctatccaga
    14221 ccgccacagc acaaatctca cagattattg ccaatgtagc gtcaagtcaa tatggtggct
    14281 gttctgccga ccgaacagat gaattgcttg ctccttttgc agaattgaac tataaaaaac
    14341 atctaaaaga cgcggaagaa tggatcgatt cgccagaacg ccaaaaagaa tatgcaaaag
    14401 caaaaaccaa aaaagatatc ttcgatgcta tgcaaagttt ggaatatgaa atcaatacct
    14461 tatttacttc aaatggacaa actcctttca cttcattagg atttggacta ggagaaaact
    14521 ggtttgaaag agaaattcaa aaagcaattt tacagattcg aatcaatgga ttagggagtg
    14581 aaaagcggac agctatcttc cctaaactga tcttcacttt gaaaaaaggt gtgaacttaa
    14641 accctgaaga tcctaattac gacatcaaac aactagcatt ggaatgtgca acgaaaagaa
    14701 tgtatcctga tattctgaat tacgataaaa tcgttgaatt aactggaagt ttcaaagtac
    14761 ctatgggctg tcgttcattc ttgcaagggt ggaaagacga aaatggtcaa gaagtaaatg
    14821 tcggacgaat gaatttaggt gttgttacat tgaatttgcc aagaatcgcg atcgaatcaa
    14881 aaggagatca gaataaattc tggcagttac tgtctgaccg cttagaaatc atgaaagatg
    14941 cgcttctcta tcgtgtagaa cgttgtaaag aagccattcc agcaaatgcg ccaattttat
    15001 atatgtatgg tgctttcggg aaacgtcttt cacgtaccga ttctgtcaat gaattattca
    15061 aaaaccgtcg agctactgta tcgttaggct acattggatt gtatgaagtt gcttcagctt
    15121 tcttcggtgg cgaatgggaa acaaacccag aagcaaaagc tttcacactt gatatcgtca
    15181 aagaattaaa agctaatgca gatgcatggg gggatgaata tggttatcat ttcagtgtct
    15241 attcaactcc aagtgaaagt ctaactgacc gtttctgccg tttggataca gaaaaattcg
    15301 gcatcatcga aaatatcaca gataaagaat attatacaaa cagtttccac tatgatgtgc
    15361 gtaaaaaccc aacgccattt gaaaaattgg aatttgagaa ggattatcca aaatattgtt
    15421 caggtggatt catccactac tgtgaatacc ctatgctcca acaaaatccg aaagctttag
    15481 aagctgtatg ggattttgca tatgacaaaa tcgggtatct tggtacgaat acgccaatcg
    15541 atcattgcta tcaatgtggt ttcgaaggtg atttcaaacc gacagaacga gggttcgaat
    15601 gtccagaatg tggaaatcat gatccaaaaa catgtgatgt agtcaaacgt acttgtggct
    15661 atttaggaaa tccgcaagct cgtccgatgg tacatggccg ccataaagaa atttcctcac
    15721 gtgtgaaaca catgaaatga ggttgtgaaa aagctgtcct gcatcaagta ataagaatag
    15781 ctaaacaaaa atagttcctc atatttttcg tttagttggg cttattactg cagatgcaag
    15841 cttttgaaca ccgtttattc agaataagag gctatgacaa gtcttttttc accaccctct
    15901 ttcgatggct ttgtatcttt gaacgccctc tatcaaaata agaacaagct ttcttgaaca
    15961 atggatttag gtcagtttgt tcttatttat gtggtcgagt agattttatc ttcaatagct
    16021 atatctttga accttaaatg aaagaagttt ttttatgaga aatccaaaac caaaagaatg
    16081 gttagcaaaa gactacagcc aaaactatat tgctgactac aaaccgttta attttgtcga
    16141 tggtgaaggt gtccgcaaca gtctttacgt tagtggctgc ttatttgcct gcgaaggctg
    16201 cttcaacaaa gctgcacaaa attttcgata tggcaaacca ttcacacctg aattagaaga
    16261 acaaatcatc gctgacttgc gcaacgatta cgtgcaagga ttaactttac ttggtggcga
    16321 accttttttg aatacagatg tttgtctgca agtcgtacga cgtatccgaa aagagttcgg
    16381 tacgacaaaa gatatctggt catggacagg ctataccttt gatgaattgc tacaagattc
    16441 agaagataaa ttagaactcc tttcgcaaat cgatatcctt gttgacggac gctttgaatt
    16501 aagcaaacgt gacctaaaac tacaatttcg cggaagtagc aaccagcgga tcattgacgt
    16561 ccaaaaatct ttagaatcca atcaagttgt gatttgggaa aaatgtacgg atgcaacaga
    16621 aacctatgaa caaatcaaaa agcaagactt gatataaaaa cagctttggg agagaaaacg
    16681 gatttccggt ttcaactctc aaagctgttt ttatatttct tgattttccc ataatggaag
    16741 gacgatgttt tcataagtca tcgggtccag attcgtcaaa gtaatcccta acaatcggat
    16801 tcctttttct actcccaaga tttcctccca tataaggctt gcttgataaa agagtgcttc
    16861 ttttttatag atatattctg gcaatgtcac tcttttggtc acagtagaat agtccgtgta
    16921 cctgaccttc aaaactactg ttttcccatg tttttggact cttctcagtg cttcttctac
    16981 tttttcagct agttggcgca actgagtcag cactgcttct tccgtttgca acggctgccc
    17041 ataagtatgc tcttttccca cagattttcg ctctcttgta acgtttactg gagaatcatg
    17101 gattcctctg actttacgat aaagagaata gcccattttt ccaaaatttc gaatcaacat
    17161 catttctgtg cattcatata ggtcttttcc tgtataaatc cctagctcat gcattctagg
    17221 tacagttttc ttccctaccc catgaaattt ttcaattggc aaagctttta aaaatggcac
    17281 agcgtcttga ggggcaacta ccgtgattcc tctaggtttt tgatagtctg atgctaattt
    17341 ggccaaaaac ttattgtaac ttactcccgc agaacatgtt agctggacac tctcccaaat
    17401 atctgcttgg atcattcggg cgattttgat ggctgacttg caattgattt tattttcggt
    17461 cacatcaaga tacgcttcat cgatactaac tggttccaca atatctgtat aacgatagaa
    17521 tacttcccgt acttgtttgg aaatatcgct gtatttatga tggtcgccag gcttaaagat
    17581 ggcttgcgga caaagctcat acgctttttg tgcactcatt gctgaatgaa ttccgaattt
    17641 tcttgcttca taatttgctg tagtcactac tccacgccca ccagtgtcac tggggtgtct
    17701 ggcaattact aatggatgtc cgcgaaactc aggatgatct cgttcttcaa ccgaagcgaa
    17761 aaaagcatcc atatcaatat gaatgatttt tcttgatgta tcattatgta aaggaaattg
    17821 cagctccatc atcctcacct cttattcatt atacaagaac attcgttcgc gttcaagaga
    17881 tttgaaaagt tagtagctga tttcctcatt agtctttcat tatagacaac ttatactatc
    17941 tcagtctaat tcatggtact gacggtagac ttcttgtttt ttcagcccat ttttcagcgc
    18001 tactgctttg atagcagcat ttggtttttc gcctgaagcg atctttgctt ctacttgttc
    18061 ctttaacgtt cccaactctt catcaacttc cgtcactggc atttcgaaca aattcccttc
    18121 tacaagcaga caacattcac cctttactgg atgttcttcg atataagcga gtagctcttc
    18181 cgtacttcct ctaaggtatt cttcatggac ctttgtcaat tcccgacaca aaacgacttg
    18241 gcgatttcca aaaatttcta agaaattcgt caatgtagca gtgatccgat aaggtgactc
    18301 ataaaaaatc agcgtaactg tctgttcttt tagctttttc aatgtttctt tctgttcttt
    18361 tttcttacgt ggaaggaagc cataaaataa aaatggttga ggcactagcc ctgaagcaat
    18421 caaagccgtc atcccagctg taggtcctgg gatagagatc acagaaatcc cttcttttat
    18481 gcaggctaaa actagctcat gtccaggatc gctgatggaa ggcattcctg cgtcactgac
    18541 ttgagcaatc gtttttcctt cttttaacaa agcaatcaat tgagggacac gttctttata
    18601 attatgttca tgtaaacttt tttggggtgt atggatttca aaatgattca ataacttttg
    18661 tgtattacgc gtatcctcac ttgcgataag atcggattct tgcagcatac gaacacttct
    18721 gtagctcata tcttctaaat ttccaattgg cgtcggtaca agatataaat ccccatatac
    18781 cggatttccc ttgaaacttt tttgattata catctgtcac aatctcctta ataaaaagac
    18841 tggagcagta tttctccgct ccagtctcct cttgttattg gtcatcgacc gctttcgcga
    18901 tagatcactt ctagacaaaa cgcacaaggc tcgtcattgt cccttcttga tccatataaa
    18961 tcgtagcaga catggaatcc ttcttcataa attttttcaa gattcatacg agacttagaa
    19021 agttcctgct tcacattttc agctggagct tgtgcaactt ggttcaattc ccgcaggtgt
    19081 tcccgcagac gttgattttc catctccaaa gttgtatttt tttctacgat ctcatgcaaa
    19141 gcatgcttca tttcaacaag ttctgttaat gtatttctca actctgattc taattgattc
    19201 aatccgtcgt ataatgatct cttatccatt catctcacct acttggataa attctttcaa
    19261 ttcatcccat tcatattcga caggattttc ccgtccagtc agccgaatac taacgatacg
    19321 gctaagcaga ttcaacccta cgactttccc tttaccatca ggtgtagcaa ttacttttcc
    19381 aaagtcaggg agttcttttt tcgctgtctc gtattcttcg ctctcatatt gcagacagca
    19441 catcaagcgt ccgcataaac cggaaatttt tgttggattc aaagataaac tttgatcttt
    19501 tgccattttg attgatacag gcacgaaatc gcctaaaaaa gaagaacagc ataatggacg
    19561 cccacatgga ccaattccgc ctattatctt tgcttcatcg cggacaccaa tttgtcgaag
    19621 ctcaatacga gtacgaaata cagcggccag atcttttacc aattctctaa agtcaattcg
    19681 cccgtccgct gtgaaagaaa aaatcaattt atttcgatca aatgtgtatt cggctttgac
    19741 cattttcatt tccaactcac gcaactgcac tttttctttt gcgattttca atgcatcata
    19801 agcatctttt ttgttttttt tgtctttttc taattcagct tgtgacgctt tatgcaagat
    19861 cggacggata gcttcaggaa ggtcattggg atcaatctct tttttaggaa ggacaacagt
    19921 agcaatatgt aaggattgct gagattcgac taataccttg tcactataaa catattcaga
    19981 gtctcctggt gcaaaataat agatatgacc tgcatctcta tatcggacac ctactacttc
    20041 taccatttcc atcctccttg atcatcatcc tttgcggcaa gctaaaaaat cgtatgcaaa
    20101 acgaactgtt ccgcaacagc ctgaaaactg acattgctgc gtagtttctg ctctgcgatc
    20161 aatatccgtt caattatctg gtttatctgt ttcactttgc catattcgcc gtttgctact
    20221 gcttgacgct gtgcttcctg ataataaaac atcaagacag ataatcctgt aaactgttgt
    20281 gttttttctt taaatgattt taccaatttt ttttggacat agataaaggc ttgagtatct
    20341 tgcttttgca agtaaacgaa ccattgataa atcaagtcct tagcatcatt aaaccattca
    20401 tcttgagaga tttcaactgc ttttcctaaa ctgtttgtta agaaagccaa aagttttgct
    20461 ttttccaatg gaatctgctc ttcttgcaat cgatggatca gtgcttcttt cgaaagttct
    20521 tgaaaatgca gaacttgaca acgagattga atagtaggga gaattcttcc tactgcatct
    20581 gtttctaaca ccgctaaaaa atctccaggg ggctcttcca agaatttcaa caaactgttt
    20641 gccgcactgc tattcatttt ttcagcttct ttgatcaaaa aaactttttt tcttgattcg
    20701 taaccgctgc gactgaattc cgtctgcaaa cgtctgatct gatcgacttt gatggtttgt
    20761 ccttccggtt caataaccaa aacatctgga tgttctttct tttcgatccg ctgacaattg
    20821 ttgcattttc cacaaggcag attgtctttc atttgggtac agaacaggtg ctgtgcaagc
    20881 catataccta cttcatgttt tcctgttcct ttctcccctt caaaaagata agcatgagcg
    20941 atacgcccat gctcaaaact ccgttgaagc tgctgataca cgatcggttg catttgagcc
    21001 aagtttattt cttgattcat agctcagctc ctcctaaaat tgatggaatc cttcaacagg
    21061 taagacgaag actgtggcac cgcctacttc tacttctact ggataaggga cgcccccatc
    21121 catcgaaata tccagtgtaa caggtgtaga tacaaattgc tttcttgatt cacacgtttt
    21181 tttgatgatt tccaatgctt cttctacacg gtcatcttca atacccacga taaacgtact
    21241 gtttccagcc ttaaggaagc cgcctgtcga tgatagtttt gtggcacgga tatttgcatc
    21301 aatcaattca tttgataaac ggttgctgtc tttgtcttgg ataatcgcta aaataatttt
    21361 cataaacgtt caccttccta gttttcaaaa aattgcggat actgttcaat aattgtatga
    21421 taacttgttt gcagcacttc ttcaaagctt tttcttgcat caactttgtg gattctttcc
    21481 ggattctctt ctgccagctt taagtaagca tgacgtacac gctggtggaa ttccaaacct
    21541 tccgaatcta aacggtcgat ttgattttgt ctgtttttct tgattcttct taatccggtg
    21601 tctgaatcta cgtcaagata aagggtaaaa tccggcgtag taccttctgt agcaaactca
    21661 ttcaaccgag caatctcggt aacgccgatg cgtcggcctg caccttgata agcaagagaa
    21721 ctgtcgacaa atctatcgca taaaacgatt ttcccttttg ccagagcagg cagaaccttt
    21781 tcgactagat gttgtcttct tgctgctgca taaagcagtg cttccgtacg ttcatccatc
    21841 cgatcatttt ttggatcaag gatcactgct cggatctctt cagcaatcgg aatgcctcct
    21901 ggttctcttg tttgaatgat ttctgttttt gctacttctt tcaaaagagg gtaaagttca
    21961 ttcaagatgc tggtttttcc tgcaccatca ggcccctcga ttgtaataaa tagtccgttc
    22021 acattgttgc ccccatctct tgtgctgctt ttatattgta tctgaaaaaa cacttaatta
    22081 gaaatttata actcacgtct tccttcgaca gctttcaata aagtaacttc atctgcatac
    22141 tcgatatcag agcctacaga cagaccatgt gctagacgag tcactttgat acctgcgggt
    22201 ttgatcagac gtgagagata catagctgtt gcttctccct ctgttgttgc atttgttgcg
    22261 ataatcactt cttttacatc tgcatcatgc agccgctgca acagagaaga aatattgatg
    22321 tcttctggac ctgtcccttc catcggtgat aacacaccat ggagaacatg ataaagtccg
    22381 tgatattctc gcattttttc cattgccatt acatctttcg gctcttcgac cactaggata
    22441 acgcttcgat cacgtgaagg atttctacat atttcgcagg gatcttcttc tgtgatgttt
    22501 ccgcagatac tacaaaaatg caaatcgcgt tttacactta acaaagattt agcaaattca
    22561 tttactactt catctttcat atcgatcgta taaaaggcga gtcttgtcgc tgttttctga
    22621 ccgattcctg gaagtttcat gtagctgtcg atcagtttcg caattggttc tggatattgc
    22681 atattcatta ctccttcagt ttaaaactaa aatcctggca aaccttttgc gtatttccct
    22741 aattttttct cagtttccac atcaatttgt gcgatcgcat cattgactgc cattacgaca
    22801 agatcctgaa gcatctcgat atcatcagga tcaacgactt cttcattgat ttggatatct
    22861 ttcatgcgac gatcgcctgt aaaaaccgct ttgaccaatt cattggtggc tttacctgta
    22921 aactctgttt tatttagcag ctcttgtgct tctgccattt ctttttgcat tttttgtact
    22981 tgtttcatca tgccttgcat attgcccatt ccacgcatca taattatcta ctccttttta
    23041 ttaatcatct accactgtaa cagcttcgga gccaaacagt tcctctgctt ttactacaag
    23101 tgtttcttct ggtacttctt gttcttgagg aagcaactcg atttctcctt ctgagtcttc
    23161 agaagcaaaa gagttttctg ttccctcttc ttgtgcctgc gtcaaataat ttcttcgaag
    23221 ttctggccag ctttctcttg taatataaac aggatcaggc gcataatctt tgatcattcg
    23281 gcttaagttg ttgtgtaccg aaagctgcaa ttcttcgtca tttgctgctc tttgacaaac
    23341 aatctcataa tcaaacgcaa tcactagtcc tgtaggtcca gctgcaactg gttcgcttgc
    23401 tttaagcatt gctcgctgag tgacagatag actcatcagc aagtcttccc atacttcttt
    23461 tacttggttc agatgctgtc ttgtcgcttc ccgcagaact ttatatactc gttccgtcgg
    23521 tacacgatag gtacttgtct gttttttatg atttgtttgc tgtaccggct gtacttttgt
    23581 attctcaatt cctgtattac tgcgtagttc cttgatttcg tttcttagtt ccttaatctc
    23641 ttgttgtagc tgttttacta tctgatcatc tgcaaatgct acatctgctg aaggaacaga
    23701 agcagacggt agctgtgcaa gtttgactgt agcgacttcc aaataaatag cggcactatt
    23761 ggtaaaacga atttcatttt gagtatcgct caaaatcttt atcattgaaa acagctgatc
    23821 cgctgaaaca gtttgtgcca attctgtaaa tgtacttctg atcgtactcg attgttcctc
    23881 aactaggtta ggtgcttgct gatacatcag caagtcacgg cagtattgta acatatcctc
    23941 tagaaagcga cgtgattctt tgccggctgc gagaatttgg ttcaatgttt ccaatgcttc
    24001 tggtacttct tttttcacgc aagcactcaa gtaactgtcc atcatttcgt cagttaagct
    24061 acctgtcact tgcatggcat catctaaagt aattgttcct tcactaaacg agattgcctg
    24121 atcaagaata ctcaatgcat cccgcatccc gccttctgca gaacgggcaa tcacattcaa
    24181 tgcagctggt tcatagctga tagtcgattc tttcaagata aattcaagat gttcgacaat
    24241 atctgccgta ttgatccgtt taaaatcaaa tcgctgagtc cgtgaaatga tcgtagctgg
    24301 tattttgtga ggttcggtcg ttgctaagat gaatatgaca ctttcttttg gctcttccaa
    24361 tgttttcaac aaggcattga aagctcctgt tgaaagcata tgtacctcat caataatgta
    24421 cactttgtac gctgcctttg taggcgcata atttgcccga tcacggataa atcggatttc
    24481 ttcgactccg ttattgctgg cagcatcgat ttcgatcaca tcttcttgcg taccagcagt
    24541 aattgatcga cacatttcgc attcgttgca tggctcaccg tctttactgt tcggacagtt
    24601 gattgctttt gcgaaaatct ttgctgcact cgtctttccg gttcctctgg gtcctgtaaa
    24661 caagtaggca tgtgaggttt tgtgggaaac aatagcattt tttagtgtct gtgtcacagc
    24721 tttttgtcca acgatgtctt cgaaacgctg cgaacgccag actcgataaa gtgcttgata
    24781 tgccataagg atcccccttt tttaaaaaat tcactatctc ttattatacg tgatattagg
    24841 taaaaaaact actatccttc tcggcttttt caggtattta aacaatacgc aattttccat
    24901 ttcaattccg tctctagtcc gagaagtttc acatcttcct atggtataat aaataaacaa
    24961 taattgtttg gcactagctg aatcagagga ggaatttttt atgggtctta tcaaagcagc
    25021 aacaagtatg gttggcggcg gtctggcaga tcaatggcta gaagtcatcg aaccagacaa
    25081 tatgagtgat acaacggtca tgacaaaagg tgtcaaagtc cgcaaagatg acaaacgtgg
    25141 ctccaatcgt aaaggaacag aagatgtttt aacagacgga acagtcgtcc atgtctatcc
    25201 aaacatgatg atgcttttgg tcgatggtgg aaaaatcatc gattataccg ctgaggaagg
    25261 ttattacacc attaaaaatg acgcagctcc ttccatgttc aatggaacac tgaaagaagc
    25321 aattgctgaa acttttgacc gattcaaatt tggcggcgtc acaccgcaga aacaacaagt
    25381 tttttatatc aatctccaag aaataaaggg aatcaaattc ggtacaagcg cgccgttgaa
    25441 ctactttgac aatttctata acgcagaatt attcttacgg gcacatggca cttattctat
    25501 tcatgtcgtt gacccgattt tattttatac aaacgcaatc cctaaaaaca aaacacaagt
    25561 ggaaatcaat gatatcaatg agcagtattt agctgaattc ctaacagcgc tacaatcagc
    25621 aatcaatcag atgtctgcag atggtcaacg gatttcttat gtaccatcta agagtttgga
    25681 attaagcaaa tacatggata ctgcattgga tgattcttgg cgtgaattac gtgggatgga
    25741 gattgtttct gtggctgtag ccagcatttc ttacaccgat gattctgtga aactgatcaa
    25801 tatgcgtaac gaaggggcga tgctaggtga tcctagcgta cgtgaaggct acgtgcaagg
    25861 ttccattgca cgtggtatgg aagcagccgg aaaaaatgaa gcaggagcaa tgaccggctt
    25921 tatgggtgta ggtatgggaa tgaatgcaaa tggttcttat ctttctcaag cagctcaaaa
    25981 taaccaagaa caaatcaaac aacaagcaga aaaacaaaat caacaaactg cacaaggaaa
    26041 tgcggatact tggacatgtc cagtttgcgg gacagaaaat tccggaaaat tctgttccaa
    26101 ctgcggagct gccaaacctg tagaaaacgt tcagcctaag ctacaaatgc gatgcagtga
    26161 atgcaatgag atcgtggatc tttccaacgg catccctaaa ttctgtccaa actgtggtaa
    26221 accgtttaaa ggaatccccg ttaattaatt tatttagaaa ggtgtgcagc tgatggatgt
    26281 tcttacacat aaatgtccta attgcggcgg tccgttgact tttgatccca aagaccaaaa
    26341 atttcattgc gaatactgcc tgaatatcta tacagaagaa gaagtcagcc aatatgagca
    26401 aaagcaaaaa gaagcgcgta tggctggcga agaaagcaaa gaagaacctt cacctaccga
    26461 agattttact tttacagccc aagaacagct tgccgatatg aatgaagccg agcgcaaagc
    26521 ggttgacgag gcaggcggtt tttcagatac agcggacgaa tctgcagata ataccgcagc
    26581 gatagatctt tttctttgtc ccaactgtgg tgcagagatc gtgactgatg cgacaactgc
    26641 tgcgacttat tgttactact gccataatcc cgtcgtacta tcgggacgtt taagcggaga
    26701 attcttgcca aataaagtac ttccttttgc tgtcgagaaa gaagaagcaa tcaacagatt
    26761 tctcgcttgg accaagaaaa aatggtttgt tcccaaagct tttttcaata aggatcaaat
    26821 cgataagttg acaggtgtgt atttccctta ttgggctaca gatgctgaag tagatggtgc
    26881 catgcaggcg aatggtacag ttatccgcat atggcggata ggtgacatcg aatacacaga
    26941 aacgaagcaa tttgctgttt caagagcagg caagctttct tttaaagaac tagtaaaaaa
    27001 tgccttatca aaaaatacgc aacaaaagat ggtcgaaggc gtgcaacctt tcccgttaga
    27061 taaagcaatt gattttaaaa gccagtattt agccgggttc caagcagaaa aaagagatat
    27121 cgaatatgag gcaatcaaat cacagattca atctgaatta aaagactatt cagagaagct
    27181 gttaaaagat accgcaaatg gatatacaac attgaccggt gtgcgaactt ctgcttcaat
    27241 cacaaacgaa gtaaataatt acgtattact gccggtatgg cttgttactt atcgaagcaa
    27301 tgactcaagt aaaaaagtct actactacgc aatgaatgga caaacaggaa aggtcagcgg
    27361 tgtcctgccg atcagccaga aaaaattggg actgaccgct cttgggattt tcgctgggct
    27421 agcgatcctc tttctgatcg gaggttactt gatatgatga aaaaattttt tttattcttt
    27481 cttttgctag taagtgttgg ctggccggtg gctactcatg cagatacagc tacggtaaat
    27541 gatgaggctg ggttattcac aacagatcaa atccaatctt tggaacaaca ggcagaaaag
    27601 ctgaatgaaa aaatcaaagg acgagtcttg atcgtaacga ctacatctaa ttcagaagaa
    27661 ccaagagact ttgctgataa ttacttaaga gacgcagtcg gaaatgatca aaatggctca
    27721 gtattgctat tagatatggg acagcgtgaa atctacattt cgacttctgg taatatgatc
    27781 gattatttga ctgatagcag aatcgattcg atcttagatg atgtttatga tcagatgaca
    27841 aatgctgcct attacaatgc tgcaaaagct tacctgtcta aatcagcaac atatgtggaa
    27901 gacggagttc caggaggaca ttatcgtgta gacgaagaaa ctggcaaaat tacacgctat
    27961 aaggttttga cgacagtaga aatcatcatt gctgttgttt tggctcttat ccttagtctt
    28021 gtcttttatt ttgtgactgt ttcgcgttat caattgaaat caggcatcta caaatatcca
    28081 ttccgagaaa aatccagtat caaattaact gaaaaaaccg atcgtctgac aaattctttt
    28141 gtcacgacaa gaagaatccc caaaagccca cctcctggaa gcggcggtgg cggaagtact
    28201 acccattcta gcggtggcgg gacttttggc ggtggcggca gaagtttcta aaaattgcta
    28261 aatgaatgtt cgcaaaaaaa gggctgtgac aaaaatcgtg tcacagcccc ttttgttgca
    28321 aataaacggc gttcaaaagc ttacatctac ggtgataagt ccagccaagt taaaaacata
    28381 agagactgtt tttacttggc tcttcttatt acttgatgca gaacagcttt ttcacaacct
    28441 cttggtaaac ggtgttctcc taccaactcc tcggtcacaa gtcacaatat tcatcaaaca
    28501 tgaaaagctg ttttttgaaa ttgttccttg tgactcagag ttaaacggca ggctcacaac
    28561 ctcttggtaa acggtgttca atcagctgac cctcggtatt agctcaaaaa acggcaaaat
    28621 atgaggaact atttttccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa
    28681 cctctttgtt catcaaaaga taatattgtc ttctaacaag tccatattca aaataacgat
    28741 atgatggtct ttaacggtga taataccatc ttttttcagt tgttttaaaa ttctgtttac
    28801 actgctagca gttgagatgc cacaaaattt ggcgatttct tcattcgtga tcgaaaaatc
    28861 gatcagcatc ccttcttctg tccgcacacc gaataaatca tataattcgt aaagttgcgt
    28921 acaaacagct cccagctttc cgtttaatag catttgctgc atttttttca tcgaataaag
    28981 cagacgatag cggtaatact cttttacata gtgttgtagc tttgggtctt ggttgatatt
    29041 ttgccaaaag cgcacacgat tgattcgata aaactctgca tattctgatt caactcgaat
    29101 attatacggc gaatcgatga attgtgaata ttcgtctttt aaaagagaga cgatttcaag
    29161 atctttgata tagcgaaggt taaattctct cccatcattt gaaataacac ttgcttttac
    29221 cgttccctct ttcaagatat acgtatggga atcttccatc ccttcgaatg tcaaatattt
    29281 atgatatcct ttgcgtataa tcggaaaatc atttttatcc aaatattcct ttaagactct
    29341 actatccaat gatgctcccc catccaaact ttcatctaaa aagatcatac agtactttaa
    29401 aactttttaa agtccaaagg tattttacta atgatattaa catttgttaa gtaccttttt
    29461 ccatcactta tgcgattttt ttcctttttt ttattcgtta tactgcagaa gtaaaatttt
    29521 ttaagaatag agggaaaaaa catgaattac tggatgaaca caatcattaa tcgattagaa
    29581 acagcatatc aaacaagatt tgatatgaaa gcttctttag tctttttaaa tgatgcgtat
    29641 caaaactcga tcgaattgat caaagcagtg gatgagaatc caacaaacga atgcgaagaa
    29701 tttttaaatc tgtttatgtc tacaagagat ttatttattc gccaacttgt tgatcgctat
    29761 ccatccaatt atcacgatgt agaagttcaa atacaaaaat taaaagcata ttcagcttaa
    29821 ggggaatgta taacatacat ggaaaagaaa ttatttaata aatattttat tggaatcaca
    29881 ttgatcaatt ttattgtcta cctgatttat tatctgttga tggtcatcat ggcagtcatt
    29941 gcgcaagaca ccttacacgc cagcttgagc caagcaggtt ttgcttctgg gatctatatc
    30001 atcggaacat tgctggcacg cttattcatg gggaaacagt tagaactatt tggtcggaag
    30061 tttaccttaa gaggcggcgc gatcttctat ctcctttcaa cattagcata tctttatacg
    30121 ccaactatcg gggcattgta tttagttcga ttcttgaatg gattcggcta cggtacagtt
    30181 tctacagcta caaatgctat cgttactgca tatatccctg catctaaaaa aggggaaggc
    30241 atcaattatt atggattaag tactagttta gctgccgctg ttgggccatt tttaggaatg
    30301 attttgctga atatgactga ttttcgtttc attatctggt tttcaattgt actggtcttc
    30361 tttgttacga ttgcctgcct tgtctttcct gtaaaaaata ttccattatc tgaagaacaa
    30421 aaaatatctc ttcgctcatg gaatatcaat agttttattg aaaagaaagc acttttcatt
    30481 acaggaattg cctttttgat gggtctttct tattcaagtg ttttatcatt tttatcttct
    30541 tatacaaaag taatccactt agttgctgct gggtcatttt tcttcgtcgt atatgctttg
    30601 gtcatcacat ttacacgtcc attgactgga aggattttcg atgtcaaagg agaacaatac
    30661 gtgatgtatc ctagctattt attcttaaca gcaggattat ttttactgag tgtaacgacg
    30721 aatagtgtga cattgcttat ctctggagca ttagtcggcc taggttatgg tacgtttatg
    30781 tcaaatggac aggctgtttg tttaaaaatc gtccgcgaac atcgaatcag tattgcttta
    30841 tctacctact ttattggttt agacctaggc ttaggcgttg gtccttattt gatggggatg
    30901 ctaaaatctc ttacttcatt tagaggattg tatgtgattg ccggagtcat tccattgata
    30961 tgtacagcac tctatcttgt taatggccga aaacatagtc atgaagcaga gggcgaattg
    31021 attgaatcat tgaaaaatga aatttgatct tgatgaatag ataaaaaaaa ccgttcagat
    31081 ctgaaaataa tctgagcggt tttttgagta aaaatagttt atgattattc ttaattaaac
    31141 atctatttat ttttaaagct ttcatttatc gataagaata gtttaactgt tgatttattc
    31201 cattttccgt tcatctggta cgatgccttg acttagcaac cacttgtgga agaaccattc
    31261 tgccacaccg atcaatagag caggaatcaa tactttcatc caaatatcga ctgtatcatt
    31321 cataaaatat agatagagcc acaagataac tgcagctgaa ccagcatctg cgatcgtcgc
    31381 tgcgatgttc cctactttac gtaagatcaa cagatcccct agcacataga tcatgactac
    31441 aataattacc ccaacaatcg ttgaatccat aaagctaaca ccgtataaac ctgtcagcac
    31501 gacccacaga atcgcccaga tcatgattgc cttcactacc aacgccttca catgtttcat
    31561 tttctgcact cctttctcct ggataaaaaa gtaagattat ctaacaagat aaccttttca
    31621 ttattatacc atcggctgag agaagcgagc aaagataagg tcctatctaa aaatgaggaa
    31681 gccacttaga acattcacgt aataatttat ccgtacagac attcgatatg gtgttccttt
    31741 actttcgcat actgttcttt tgtcagttct ttacttgtca ccagataatc gatttcatga
    31801 aaagcaatgg cttgatgcat gaatttatgt gcaatttttt gctgtgtaac taaaacggca
    31861 agccggtctg attgttttct aaaagctttt ttgaccattg cctctccttc atgagtttct
    31921 gttactcctt ggctgaaatc tagcccgcga caactcatta tcgctaaatc cgcataatat
    31981 gacaatgcat cgttataggc ttttgaaccg ttgattgttg ctcgtttcgg aacaattgcc
    32041 cctcccaata cagatacttt tgccgttgta tattcactca acatagcagc tgtgacgatg
    32101 ccattcgtaa tgatctgcaa accatcaaat cttgctaggt ttctgactaa gtgtaaagag
    32161 gttgtactgg aatccaagaa taagaccata tcatctcgaa tcaaagaaga agctgtatct
    32221 gcaatcttac gtttttctgt ttcttcttct aatttggtga tcttcatcag gtaaggcacg
    32281 tcaatttttc gattgtcaat aagcatggcg ccaccgcgaa ctctacggat caatccactt
    32341 ttttccagaa aaacaaggtc acgtttaatc gtggcttcgc tataatttac taactcgatc
    32401 aaatcggtga ctcttgcaaa ttggttgttt tttagataat tcacaatctc ctgctgtcta
    32461 ttctccactt cattcaacat aaaatcaccc catttacttt atatagtaac aggaaaaaag
    32521 aggttgtgac agaagtgatt ggcttcgaga aataagaaga aatttttcga aaaaactact
    32581 tctgaacatc gtgtgtggtt aagagggtat gacaagacta ttgtcatacc ctcttgacca
    32641 cacattttta tttttagtat aaactagcct tgcctactgt gttcaaaatt tccattttat
    32701 gtttgaccac ttcttgtacc tttttattag ctgctggata aattgcattc ggctcgtaca
    32761 tcgatggatt ttcactcaat atttctctca aagcattgaa aaagacgctt ttaagatctg
    32821 agctcaaatt cactttggca actccgtatt ttactgcttc gctgacttct gcgtccggat
    32881 tacctgaacc gccatgtaaa acaaatggaa gatcgatttg ttggttcaat tctttcagta
    32941 agtcgatatt taattttgga gttctgtttt tcggatacaa gccatgcgct gttccaattg
    33001 ctacagcaag tgtatcgatt cctgtttctt tcacaaaaat cttcgcttga tcaggatctg
    33061 tataaatgat tgtttcagca cctccttcag cagaaccatt attcccaatc gttccaagct
    33121 cagcctccac tgagacattt accgcatgag ctaacgcaac cacttttttc gtaatctgta
    33181 cattgtcgtc gaatgatttc aaagacgcat cgatcatcac ggaagaatag ccgtttcgaa
    33241 tcgcccgtaa gacatcttct atcgtaccgc catgatccaa gtggataacg atcggcagtt
    33301 tacttgtata agcataggct cttactgccg caacaaactc atcacttaaa taggcgattt
    33361 catcaggatg aatttctaag attactggtg cttctaatct ttctgcttct tcgatgattg
    33421 ctttcatcat atcatagctg caaatattga aggctggtac tgcaaattga ttctctttag
    33481 ccactttcaa taactctttc attgtatata acattacgct tccacctcat ttgttagatt
    33541 ttcatttttc gtccacttgt cttgtttttt ctctttcact gtttttttca atagtccaag
    33601 cataaaggct gtagtgactg tacctgatgc aattgccaag acatacatag gtaaattgtt
    33661 cataacattt ggaattagca tcacccaaat ccctccatga ggtgccattg aagtaactcc
    33721 tactgccatg gaaatccctg cagcgactgc tgaacctgtc atcaaactag gtatcacgcg
    33781 gatcgggtca gataccgcaa acggtatagc accttctgta atatacgaag cgcctaaaat
    33841 ccagcatgat tgacctgaag tccgttcttc tttggtaaat ttatgtttga ataataaagt
    33901 ggctaaagca atacctaggg gaggtgtcat tcctgcagcc atacaagcag caatcggtgc
    33961 atttactcca gctgacatca aaccgatcga gaaagtcgaa gtcgatttat tgatcggccc
    34021 acccatatca gctgccatca tgatcccgat aagtaatccg aacaatagcc cggatgtatt
    34081 tcccaagcta ttcagccaat ttgtcaaagc atccaacacc catgtgatcg gttgttcaat
    34141 cacaaatacc atcaaaagcc caataattaa gatcgataaa aaggggacga caatcagctg
    34201 ataaatagca gaaaattctt tttttacgct aattttattt gccagaaaat cacagatata
    34261 ccctgctagc acgccaccaa tcattccgcc aagaaaacct gaaccacctg tagaggccag
    34321 taatccggca acaattcctg gggcaaaacc tgcttttcca gcaatcgatg tagcaatccc
    34381 cgcgcctaac gcaggaacca tcatggcaaa cgctgtatct cctccgatcc ttgaaaatgc
    34441 agctgctaac acattatagt ctgctgagtt gggatcagcc gctgtaatcc caaaggcaaa
    34501 actcagtgcg atgatgattc cgcctgcgat cacaaatgga agcatgtaat tcacaccatt
    34561 catgaaatga ttatagattg tcggcttttt ctcatttgca gctggttctt tttcattaga
    34621 cacaacgaat acgccaatat ctctcaatgc ttcgtctatt actacttgcg gttctttgat
    34681 tgctcggctt gtagagacac gctttacttt tttcccggca aatcggtcca attcgatttc
    34741 tttatctact gcaagaatga ttgcatctgc ctctttgatt tcttgttcag ataactgatt
    34801 ttctgttttt acaccttgtg tttcgaattt tgcttgatgg tttagatttc ttgctgttac
    34861 ttctaatttt tctgccgaca tataagtatg agcaattcca gtcgggcaag cagttacacc
    34921 aacgattttc atgattattt tcctcctttt tcttcactat cgatactcgg acaaagactc
    34981 tactattttc attcttgact aatctgtctg atgatatctg ctggcgtagt ggcttctttt
    35041 attccctttc taaaatctgc attcatcaat cgtctggaga gtgtgctgag aatctctaga
    35101 tgcatatctg ctgatttttc aggaactacg atggcaaaaa tatattcaat tggcatttca
    35161 tctatcccgc tatagtccgt taatttggct gtatgaaaaa aacacagtgc tggttttgtc
    35221 acatatttgc tttttgcatg gggaatccca aatccatctt ctattcctgt ggagatctcc
    35281 tcctctcgtt tgaatagtgc cttaacgact ttttgaacat ttcttacctt atttgttttt
    35341 ttcaacacat cccctattcg ctcaaataat gatccttttg aatctatcga tggatcgaaa
    35401 acaatcagtt cttctgcgat caattgatca aacctcataa aaaacctctc ccccacaaaa
    35461 aatgttaacg ttttcttgga actgaggtca gtatagcatc gctagctgta ccgttcaata
    35521 ttatgaatga acagaggaaa aagacccatc atccgcgtgt ataagtcatt aaaatgaacg
    35581 atctagctca caaacaatcg caagtctata acaaaacaaa aagccttcct tgaaaggaaa
    35641 gcttagcttc taattttagg taaatggttt tttacttcga aataacccct cttcatttcg
    35701 ttaatataag gtgtttttat caagtggata attaaaacta tatactagtt tttcattgtt
    35761 ttgtgcatta tgatcctacg actttttcag catcaaacga agttttaaat tggcaaaaaa
    35821 cataaaaaaa cagactatcc ctatcagata gtctgctaaa cgttgagtta tcagcgtttt
    35881 tattattccg cagcgtttgc gtctttgaga ccgtattttt tgttgaaacg gtccacacgt
    35941 ccgtctgctt gtgtgaattt ttgacgtcca gtgtagaatg gatgtgagtc agaagttact
    36001 tcgacacgga tgactgggta tgtattaccg tcttcccatt caactgtttc ttgtgaagtt
    36061 tttgttgaac ctgacaagaa tttgaaacca gttgttgaat ccatgaatac tactgggtgg
    36121 taatctggat ggatgttttc tttcatgatt ttttcgctcc tttgccctgc tccatttgct
    36181 gaaccagagt tgatttgtcg atttacaaca ttaacatctt aacatgtttc agtcaaggtt
    36241 gcaattatct tttcagattt ctggtcggat tttttttacc aaaggagaca tcttgaaatt
    36301 ctttgaaaaa ttgctgattg ttttttgttt tacgcaaaaa gcggatcagt tgttccgtat
    36361 attctaatga atctccagtc atattttttc gaagtttcca aatttcttct aattcttcac
    36421 ttggcatcaa taattcttct tttcgggtac ctgatttttt gatatcgatg gctgggaaga
    36481 tccgacgttc agataattca cgtgaaagtt gtaattctag atttccagtt cctttgaatt
    36541 cttcatagat cacgtcatcc atacggcttc ctgtatcgac taaagcggtc gctaaaatcg
    36601 tcaaactgcc accttcttcg atgtttcttg ctgccccgaa aaatttctta ggtttataca
    36661 atgcggctgg gtcaatcccg ccactcaatg tacgtccgct tggtggaacg actaagttat
    36721 aggcccgagc aagtctagta atactatcca tcaagataac gacatctcgt ttatcttcga
    36781 ctaaacgcat tgcgcgttcc aatactaatt cagaaactcg agtatggttt tgcggctgca
    36841 gatcaaatgt agaagatacc acgtcgcctt tcacgcttcg ttctagatcc gttacttctt
    36901 ccggtcgttc atcaatcagt agaacgatta attctacttc cggatagttt tcggtgatac
    36961 catttgcgat ttcttttaat acagaagttt tccctgcttt tggcggtgcg acgatcaatc
    37021 cacgctgacc aaaaccgatt ggagaaaaga tatcgatcat tcgggtagat agtctttgcg
    37081 gtgttgtttc cagttgaagc tgtctttgcg gataaagagg tgttaaggct ggaaaatgcg
    37141 gacgttcttt tgcttcttcc ggatcttttc cattaacgct ttccacgtgc atcaaaccat
    37201 aataacgttc cgattcttta ggtggacggg cttttcctgc cactttgtcc ccatttctca
    37261 aactgaatcg tctgatttgt gaggcagaaa tatagatatc ttctgcactt ggtccataat
    37321 tgattggccg taaaaaaccg taaccatctt gaccaacgat atccaatatt ccttccatat
    37381 aataaaatcc ttgtttctct gcttgtgcac gaatcacagc tagagataat tcctttttat
    37441 tcatcttact gtaataagga attttaaatt ctttggcata actataaact tcttttaacg
    37501 ttttgttctc tagttctgcc attgtcaaat aatcgctcat tcagctccca cttcctctat
    37561 catttcaatt gatgccccta aggctgttag tttctcgata atatgatcat aaccgcgtaa
    37621 gatataatct acattgtaaa tcgtcgttgt cccttctgcc aatagtccag caatcactaa
    37681 acaagcacct gcccgaagat cagaagccac gacttctgtg ccgtgaagtt tatttggacc
    37741 attcacgatg atcatatttc cttccacggt aatatcagcg cccattcgtg ctagttccgg
    37801 cacatggttc acacgtttcg catagatcgt atcgatgatt tctgaagtgc ctgtagtcat
    37861 caatagtaac gcagtaagtg gctgctgcag atctgtggca aatcctggat aaggataggt
    37921 catgatgttt gcgggtttta attctttcga ggggtaaact tcgatttcat cttcgctgat
    37981 tttcatatgg acaccaattt cctgcaattt tgctataaag ctttccaaat gttcaaagat
    38041 cacatttttc actttgatac catttcctac tgcagctgct aatgctagat aggttcctgc
    38101 ttcgatgcgg tcaggaatca tgaaatgccg acagccatgt aatgtttcga ctccttcgat
    38161 acggatcaca tctgtacccg ctcctcgaac ttttgccccc atattattca gtaaagttgc
    38221 cacatctatg atttccggtt ctcgtgcagc gttttcaatt actgtctgtc cttttgcttt
    38281 tacagcagct aacatcacat tgatcgttgc tccgactgaa accacgtcca tgaaaatccg
    38341 attgccacgc aatcctttat tttctgtccg taaatacatg gcaccatgtt catttgtcac
    38401 ttcagcacct agagcttcaa atcctttgac gtgtaggtca atcggtctag ggcctaaata
    38461 acagccacct ggcaggccta ctaccgcttc gccaaattta ccaagcagtg ttcccataaa
    38521 ataataagaa gcacgcaaac tattgatttt tccgtgcggc atcggaatcg atacgacttc
    38581 tcgaggatcg atttctagta tgttgttgga aaaatggaca gttgcgccca tgatctctaa
    38641 aatttcaatt aatgaatgaa catcttgtat gtccggcaca ccttccaaga tcactgggga
    38701 atcagctaag attgcagctg gaatcaatgc gactgcacta ttttttgcac cgctgatcgt
    38761 tacttcacca gctaaaggtc ggtttccttt gattacgatt tttttcatct gtttccacct
    38821 tgtttattaa tcttttttca tggatatgct tgtattttat catagataaa aaaaaagaaa
    38881 caagcatggt ctgccgtttt aagtaaaaaa gaatggagag cgacttcaaa agtcgttctc
    38941 cattcctata tatcgtatct gttatgtgga tgcttacgct ttgttagctg aaccgaacca
    39001 gtcgatatga gatttagcat cttcaacgat tgctgcttta cctggagcta ataatttacg
    39061 agggtcgaat cctttacctt ctttatcttt accagcttcg atatatttac gagtagctgc
    39121 agcaaatgat aattggaatt cagtgttaac atttactttt gagataccca ttgagatagc
    39181 tttttcgatt tgatcttgag gaatacctga tccgccatgt aatactaaag gcatgtctgg
    39241 acctacagca tcagcaattg cttgtaagtg gtcaaaagct aatcctttcc agttttctgg
    39301 atattgacca tggatgttac cgataccgca agctaagtag tcgatacctg tagcaaccat
    39361 ttgtttgcat tcttcaatat cagctaattc gccgttaccg atgattccgt cttcttctcc
    39421 gccgattgaa ccaacttcac attcaactga gatacctttt gcatgtgctt tttcaacaac
    39481 gtctttagct aatttcaagt tttcttcaaa tggtagatgt gaaccatcga acattactga
    39541 agtatagcct aattcgatac attccaaagc agcttcgtaa tcaccatggt ctaagtggat
    39601 agcaactgga acagtgatgt tcattgcttc aactaaatca gtgattaaat ctttacagat
    39661 tttgtaacca cccatgtatt tagctgcacc cattgatgtt tggataagta ctggtgcttg
    39721 ttttgcttca gccgcttcaa ggattgcttg agtccattcc aagttgtttg tgttgaatcc
    39781 gccgacagcg taaccgcctt tacgagcagc ttttaaaaat tcagctcctg atactactgg
    39841 cataataaaa ttcctcctaa gttttgtaaa actaaatttg ttaagacaat tcttaaccaa
    39901 cgaccctatt ttaccaaaca tttcggtagt tttctactaa aatcaatgaa aatgaaaatt
    39961 ttcaagaagt tttttgcaaa aaataaaata tccaatagcg ctttaatcga aaaaagtctt
    40021 ttgctttcta tcttttttcc tacaaataat aacaaatcag aaaaagagaa aagtgacaag
    40081 aaaattgctt atccccttct cttgctactt ttctctttct atcaagttca aaaattattt
    40141 tgtttcgtga tctgctaatg atgctccgac aaatgctttg attaattttt gcgggcgatt
    40201 tggacgagaa atcaattctg ggtggaattg gcatcccaca aagaattttt tctctggcag
    40261 ctctacgatt tctactaaac ggttatctgg tgagacacct gaaaagacta gcccttgttc
    40321 ttcaaacaat tgacgatatt tgttattgaa ctcatagcgg tgacggtgac gttcttgcac
    40381 aacatcggct ccatcatatg cttcagccgt cttcgttcct tttttgattt tacatgggta
    40441 taatccaagg cgtaaagttc cacccaaatt ctcgatgttt tcctgatcag ccattaaatc
    40501 aataatattg tttgctgtat ctggattcgt ttcagctgat gacgcatctt ctaatccaac
    40561 gacgttacgt gcaaattcga cacaagccat ctgcatacca agacaaatac ctaaaaacgg
    40621 aacgtcattt tcgcgagcat aacgaatcgc ttcgattttc ccttctactc cgcgatcacc
    40681 aaatccacca ggaaccaaga ttccatctgc gtctttcaaa cgttcttcta cattttctct
    40741 cgttaattct tgtgctttga tccaatcgat ctcgatgtct gaattatagg cgaaaccaga
    40801 atgtttcaat gcttcaacta ctgagatata tgcatctggt aactcgacat atttaccgac
    40861 aagcgcaatc cgtacttttt tcttcaagtt caagacacgt tcttctaatt ctttccattc
    40921 tgtcatgtct gcttctggtg tatctagttt taaatggtcg caaacaattt gatccattcc
    40981 ttgtgcttgt aaaactaatg ggatggagta aagtgtatct acatctggcg actcgataac
    41041 tgcttctggt tcgacatcac aaaattgtgc caatttgttt ttcacgcttt gagaaacagg
    41101 ttgctctgta cgtaccacca aaatattcgg ttggatacct aaaccacgta gctcttttac
    41161 actgtgctgc gttggtttag ttttcatttc tccagcagct ttcaagtaag gaatcaatgt
    41221 tgtatggata tacataacat tatctgtacc gacatctgct ttcatttgac gaagtgcttc
    41281 aaggaatggc aaagattcta tatcccctac tgttccccct acttctgtaa tgatcacatc
    41341 tgaatcagtc attttagctg ctcgcatgat tttttctttg atctcatttg taatatgagg
    41401 aataacttga acagttgctc ccaagtattc gcccttacgt tctttacgca aaacttcaga
    41461 atagattttt cctgtcgtca cgttggagta tttgttcaaa ttgatgtcaa taaaacgttc
    41521 gtagtggccc agatccaaat ctgtttcagc accatcatct gttacgaata cttctccgtg
    41581 ctgatatggg ctcatcgtcc ccggatccac gttgatgtat ggatcaaatt tttggatagt
    41641 tacttttaag ccgcggtttt tcaataagcg tcctaaagaa gctgcaacaa tcccttttcc
    41701 gattgatgaa acgacaccgc cggtcacaaa aatatatttt gtcataagtt caaaaactcc
    41761 tttttaattt atgtgcagga aaagtctcag acaataaaca aagctcccta ttcttcaata
    41821 agaataggga gccgaaattt ctgtaaactt ctgaccctct aaaagggtgc ccaaggtgta
    41881 taatacaagc tgactaaggg aaagtcaagt actcaaatga atactatccg cttacaattt
    41941 tttctttttc gatatccttt gagaa
//
LOCUS       DAYYHX010000005        33434 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000005 DAYYHX010000000
VERSION     DAYYHX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.005"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..381
                     /locus_tag="ACHA6Y_000178"
     CDS             61..381
                     /locus_tag="ACHA6Y_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM8986306.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(493..1350)
                     /locus_tag="ACHA6Y_000179"
     CDS             complement(493..1350)
                     /locus_tag="ACHA6Y_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8986307.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(1458..2762)
                     /locus_tag="ACHA6Y_000180"
     CDS             complement(1458..2762)
                     /locus_tag="ACHA6Y_000180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8986308.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(2759..3268)
                     /locus_tag="ACHA6Y_000181"
     CDS             complement(2759..3268)
                     /locus_tag="ACHA6Y_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986309.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(3443..5578)
                     /locus_tag="ACHA6Y_000182"
     CDS             complement(3443..5578)
                     /locus_tag="ACHA6Y_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8986310.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(5661..7316)
                     /locus_tag="ACHA6Y_000183"
     CDS             complement(5661..7316)
                     /locus_tag="ACHA6Y_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8986311.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(7318..7632)
                     /locus_tag="ACHA6Y_000184"
     CDS             complement(7318..7632)
                     /locus_tag="ACHA6Y_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8986312.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            7802..8062
                     /locus_tag="ACHA6Y_000185"
     CDS             7802..8062
                     /locus_tag="ACHA6Y_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM8986313.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            8076..8390
                     /locus_tag="ACHA6Y_000186"
     CDS             8076..8390
                     /locus_tag="ACHA6Y_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM8986314.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(9120..9674)
                     /locus_tag="ACHA6Y_000187"
     CDS             complement(9120..9674)
                     /locus_tag="ACHA6Y_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM8986315.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(9800..11729)
                     /locus_tag="ACHA6Y_000188"
                     /pseudo
     CDS             complement(9800..11729)
                     /locus_tag="ACHA6Y_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(12035..12751)
                     /locus_tag="ACHA6Y_000189"
     CDS             complement(12035..12751)
                     /locus_tag="ACHA6Y_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8986316.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(12752..14575)
                     /gene="glpO"
                     /locus_tag="ACHA6Y_000190"
     CDS             complement(12752..14575)
                     /gene="glpO"
                     /locus_tag="ACHA6Y_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM8986317.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(14589..16085)
                     /gene="glpK"
                     /locus_tag="ACHA6Y_000191"
     CDS             complement(14589..16085)
                     /gene="glpK"
                     /locus_tag="ACHA6Y_000191"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM8986318.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(16221..17681)
                     /locus_tag="ACHA6Y_000192"
     CDS             complement(16221..17681)
                     /locus_tag="ACHA6Y_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8986319.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(17671..18966)
                     /locus_tag="ACHA6Y_000193"
     CDS             complement(17671..18966)
                     /locus_tag="ACHA6Y_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8986320.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(19298..19975)
                     /locus_tag="ACHA6Y_000194"
     CDS             complement(19298..19975)
                     /locus_tag="ACHA6Y_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM8986321.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(20005..21096)
                     /locus_tag="ACHA6Y_000195"
     CDS             complement(20005..21096)
                     /locus_tag="ACHA6Y_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM8986322.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(21197..21631)
                     /locus_tag="ACHA6Y_000196"
     CDS             complement(21197..21631)
                     /locus_tag="ACHA6Y_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8986323.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(22215..23672)
                     /locus_tag="ACHA6Y_000197"
     CDS             complement(22215..23672)
                     /locus_tag="ACHA6Y_000197"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8986324.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(23669..25564)
                     /locus_tag="ACHA6Y_000198"
     CDS             complement(23669..25564)
                     /locus_tag="ACHA6Y_000198"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8986325.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(25622..27397)
                     /locus_tag="ACHA6Y_000199"
     CDS             complement(25622..27397)
                     /locus_tag="ACHA6Y_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8986326.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(27447..28943)
                     /locus_tag="ACHA6Y_000200"
     CDS             complement(27447..28943)
                     /locus_tag="ACHA6Y_000200"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8986327.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(29137..30759)
                     /locus_tag="ACHA6Y_000201"
     CDS             complement(29137..30759)
                     /locus_tag="ACHA6Y_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8986328.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(31087..32400)
                     /locus_tag="ACHA6Y_000202"
     CDS             complement(31087..32400)
                     /locus_tag="ACHA6Y_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8986329.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(32645..32869)
                     /locus_tag="ACHA6Y_000203"
     CDS             complement(32645..32869)
                     /locus_tag="ACHA6Y_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986330.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(32889..33311)
                     /locus_tag="ACHA6Y_000204"
     CDS             complement(32889..33311)
                     /locus_tag="ACHA6Y_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8986331.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
BASE COUNT         9575 a         7148 c         5610 g        11101 t
ORIGIN      
        1 aaatatgtgt agtgcttctt gaagtttgtt atactttaat taaaagagag gagtgatcga
       61 atgattacaa caacaacgaa cagtgtagag ggaaaagccg tagtagctta taaagggatc
      121 gtttttggtg aggtcattac tgggatccat gcatttaaag acttggaggc ggggttgcga
      181 aatatctttg gcggccgctc aaaaagctat gaaaacgaac taatgggtgc aagagaagaa
      241 gcacttgccg aaatggccga acgtgcaaaa gcattaggag ctgatgcgat cattggcgta
      301 aaaatggact atgaagtatt aggcagcgac aatggcatgc tgatggtcac atgtagtgga
      361 acagctgtct ccacgagata agtaggggac atctgtttta ataactaaag agagatggga
      421 catgtagtgg cactcaagaa ttagattttt cagtctaact tcgaggtact ctaccttccc
      481 atcctctctt ttttatttct ttttgcggat gattccgtaa gctgctgcga ctaacacacc
      541 cgcccctgct aaggaataac tgacagaagc tgttttttca tttgtttttg gcaaagtctg
      601 acttgttgat ttggatggct ctgtcgtttg ctgaactgtt tctgtcgtgc ttccagaggt
      661 cactgatttt tctcctgccg aatgcaaaat atcttttatt cgttggaaac tagctgctaa
      721 gtcagctttt tttgcttcaa aatcattagc aactgtttgt gcttcgctga tctttccgtg
      781 gattgcttga ctggccgctg tgatatccgc ttgagtttga cttagctttc cagaagctgt
      841 ttgagcaacc tgatttaatt gctgattgaa ttcttttgct tgttggatct tcccgtcaag
      901 atctgtttgg acagcttccg cttttgcttt cgcttcggca agctttttct caagttgtgc
      961 acggtcattt cccactttat ttttaagatc agctaaatct tgatcgattg ctgcttggtc
     1021 ttctttcaat ttgttttttg cagcttctaa tgcttcttcg agttcttttc ttttattttc
     1081 caagtcttgc aaatgatcga tttggtcaga agaggtagaa ctatttattg atgtatcttc
     1141 tgaagatgaa atcgtttctc ctgaattatc tgtcggtaca ttggaagtca tttctgtttc
     1201 cgaggcaccg ttttcagcac cagaagagct tatggttgtt gaaggttctg tttgagcagg
     1261 ttcagaagtt tccgcaaaag ctgtctgtcc caataagaaa atactggaaa cagcaattgg
     1321 ccagattaac ggcttcattc tttttttcat cacttttccc atccttacta ctttttaact
     1381 aatttcttat tataagcaaa aatgtttgga agaaccataa acacaacagg ctgtttgtct
     1441 aaaaaacggt gcatctttta tttttttgta cgttccgggc ttttccatgt cccgcttcca
     1501 taacctaaaa tcgttttgac tgctggaatg aaagtcgtca cgatcgttat cgcgttcact
     1561 atccagaaca aaagcatata tagtggtgcg aaaagaaggt atttcaattt tcttcgtcga
     1621 tcatctacaa tcagcgaagc tagcaactgg aaaaatcctg cgaccatttc aaagcagatg
     1681 aacacaaaac tgaatgtaaa agtaatataa atctgttcgt agtttccttg atatacataa
     1741 tgcccgatta atccagcaaa aagaactacg gaaatacaaa agaatattga ccagacaata
     1801 ctcaatgttt gatcaatgaa gatgatcgtt cttccaatat gtttaaaagg atggagcatc
     1861 acttttttga aattcgtcag ccagacttct gttccacctt ttgcccaacg ttttctgtgt
     1921 cgatatagca tcttcaacgt cacaggtact tccatgaaaa agataatctc aggagcaaaa
     1981 acagataacc agtcatttaa ctgatgatcc caagcaatac tgatgtcttc tgtcgctcga
     2041 tcttgtctga atccgcccac atcgatcaag gcttcttttc gatacatcgt atttgctcca
     2101 ctgtacgcat aaagaccacc gaaaacagct gactgtgttc gtttaatgat gcccacgata
     2161 cttgagaatt caaccgtttg agattttgca atcaatttgg tacgattttg gacatccata
     2221 tttgcagtga ccgcagcaat atgtcttgca ccagggcgaa tgaaataatt cacataccta
     2281 atcagtgcat ctggctccgg taccgtgtct gcatcattgc ttaaaatcaa tttccctttt
     2341 gcaaaggcaa gtccgatatt aaacgcgtgg gcttttcctt tatttttttc gatccgaacc
     2401 acacgaagat tagcgtattt tttcatcagt ctagctaata tttctggtgt ttggtccgtt
     2461 gaaccatcgt ctgtcactaa tacctcatag ttgtgataat tcaatttcgt catcaaatac
     2521 tcgattgtat attcgatcac aatttcttca ttatgggctg gcaccatgat cgtgatgaac
     2581 ggttcgacac tcaagggaac atctacccat tctttttgct tatgtttgta taaaaacaca
     2641 taacaccaga caccaataaa ccatacaaat ccgcctaaaa tcggataaga aacgagaaaa
     2701 aaatacataa tagacaaaca agtatctaag aataaattac ctgtatgggt gattgagatc
     2761 atttcagttc caccccattt tctttataca aatctgcaat gaatgtcgta tctagatttt
     2821 tctcttcgga gacagaataa aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt
     2881 ccgtatacac ttcttcaagt aattcttgtc tttttttcaa tttttcatct tcatattcaa
     2941 aagatttatt tacttttttg tatagatttc gattgttcca cctagtcaat acaaatgagg
     3001 taatcaggaa actgaaaaaa atcaatacaa aaaagccgat taagaaatac agcattttct
     3061 tttctgataa aaaagcgtac aaggaaaaat ccttggctct ttctggtctc gttaatggga
     3121 aaaagaccca tataaaagga agaacaacga tcatccatcc taaaagagcc actaacgttt
     3181 gccaaatctt taaccagtaa ttaccttttg aaaaatactt atctttcacc aaaattgatg
     3241 caacttcttc tcttgttact tttttcatat cctcacctca cgtttttttg ttacgacgta
     3301 ggagtttacc atacatttca tttcttttca cataaagtgt tcctaaattt ttcctaagaa
     3361 aaagaacaaa acatttctca tcagcaaatg aaaaatattt tgttcttttt atcagttgtt
     3421 ctttttattt tttatctctt aattatgttg ctctttccaa tacgacaatg cttttcattc
     3481 ctctagatat cgccgtgtac atttgtctgc gtcttctttg ctcatctcca tcgttttcaa
     3541 atggatataa gattacatga tcaaattcca gtccttttgc tagactcaaa ggaatgattt
     3601 ggcagttttc taatccatat gaattttttt taatcgattc ttgtagcgtt tgggtttcct
     3661 cttctgtttg ggtgataact gccgcttttt ctccttttag agaaaccaaa atctcagcta
     3721 gtattttcaa caattcttca gaggatcgaa aagagcggta ttcgggttct tttccttgcg
     3781 gtcggatcga tacgatgtcg atcttctctt gatccacagc ataggttttg aaaagctctg
     3841 tgattgctcc gctagaacgg taactatttc taagatcata ccgatggatc ggcagatggt
     3901 agtcgtcgaa acaacgcata atatcagcaa aagtagtgga agaattaaag attgcctgat
     3961 tctcgtcacc tactaaagta aagtgagttt ttggaaataa ttcgatcaat agacctaatt
     4021 gtgccagggt ataatcttgt acttcatcaa tcaatagata gcgaaatgct ggtacattta
     4081 ccttttcaac aagtaaatga cgaatagcta atataatgac tgcttcatca acagtacgtg
     4141 tgggttgcca agcataagct gagccatatc gtttttcata aattctttca aacaactgat
     4201 gctcatccac ccatgccatt tcttcgattt gtctggttat ttttcgatat tttttttgta
     4261 aaagtttcct tgcataagct acaatacttt gttccgaatc atcagaaatc aatttaccga
     4321 ataattccaa ctgcctatct tctgtcaacg agagtacctg attctgcaca gcaggacttt
     4381 ttgcttgttt caaaagatga ttcttccatt cgctcatcag tgcctttttc gttgcttgaa
     4441 gcctatcgat caatttagga tgagcaggaa ctttctcata tattttttcg atcgtttttt
     4501 tagaaaggac tgtttttcca tttttagtaa gtccacggat aaaattcgga tgatctaaaa
     4561 acaaaggatc tgattgtttt agattatcga taaacttttt gctacgcaaa cgttctgtct
     4621 gctccgatac attttctccc gtgatacgtt tgaaataagc ctcttcacct tctatttcct
     4681 ctgctgaaag ctgtgaaacc agctgtatga tggttagatt cagtggattt ctttcaccta
     4741 atgatggtag gacattcgaa atatagtcga taaaccgatg attaggtgat agaatcagca
     4801 agtcatcgga tgtcatcgta tctcgatata aatagagcag atacgcaata cgctgcatga
     4861 tagcagaggt tttaccactt cctgctacgc catttaccaa gatattttga cttttcacat
     4921 cccgaatgat tgtgttttgt tccgattgga tcgtagcagt gatagccttc atttcattgg
     4981 tctcatcttg gcctaagact tctaataata cgtcatcttg aatagctaca gacgtatcaa
     5041 aaaacttgac caaacgatct ctttccaaaa gaaattgtcg tctttcttta ataaccgtat
     5101 cgattttatt ttgacgaacc gtataagaag atggaccaat catgttattg taaaaaagtt
     5161 cggcaattgg tgaacgccaa tcataaacaa gattttcatc tgcatcatct gtgaaatttg
     5221 ctgtaccgat ataaaaggat tcttttggct cattatccag aaagtctaca acgacttttc
     5281 caaagtaggg acttttcaat aaacgttcga tctttgctag ctgattttct gcgtttttct
     5341 gtttgatatt catctgatcg atttctctat ttttcatttc aatcatcgaa aaagtatcca
     5401 ggttatctaa gtaactgtca aaattcaatc gaagctcgcc agccatttct ttcaacgact
     5461 ggtttccttc tatttttgcc gtatccatcg attcccgcag ttcgtttttt ttatcaatta
     5521 gcttttgata cacgttcttc agatatgctt cttctaactg tttttgtgtg ttgaccatac
     5581 acattctcct ctttttttaa agtggtggaa ccgattagtt tacctctttt aagagtcgaa
     5641 tgcaagcttt ttaacgagta tcatttgtta ggatatatca gttcttcagg tcggacagtt
     5701 tgatataaag aaaatgcacc atcaaggttt tttacgtggt agccagactg cttcaagatt
     5761 ctttccgcta gatagctacg taatccgcta tgacagctga cgatatattc ttgttggctg
     5821 tctaattcat ttaatcggtc ccgaagttca ttcaaaggga tatgtttggc ttccttgaat
     5881 ttgccctttt ccaattcttc tgctgttcgc acatctaata aaatttttcc ttttgctagt
     5941 tctgtcggca attcatgcca ttgtacggtt tcactaagcc cttctgcaat gttcattgct
     6001 gcatatccta gcatgtttac tggatctttg gctgagccaa atggcggtgc atacgtcaat
     6061 tctaattccg gtaaatcgaa gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg
     6121 atccgtttat ccacaccttt tgccccaaca ccttgtgcac cataaatctc gccagtctct
     6181 ggatggaaga tcaacttcag taaaatatct gttgcttctg gataatagct tgcatgatct
     6241 tttcctgata catgaatgac ttcaaaagac agttcgtttt ctttggctgt tctttcactt
     6301 aaaccggtgg atgcggcagt cattccaaac gttcgtataa ttgctgttcc aataccgcct
     6361 tgatttcttc gagaaattcc ggaaatcgtg tccgcaactt gtctaccttg acgattggct
     6421 ggtgaagcaa gagaaataag tgcatctttt ccagtgattt gctgttttac aacgatagca
     6481 tcccccaccg cataaatatc aggaatattc gtttgataac gttcgtctac taggataccc
     6541 ccgcgtatgc ctaattcgat tccagcggtc actgctagct ggttatcagg acgaacacca
     6601 atggataaaa taactaaatc agcagggatg gtacgcccat catccagtcg caatcgtttc
     6661 ccgtggtctt caaatccagc gacagcatga gaagtaatca cttctactcc ttgatgagac
     6721 aattcttttt ctataaaagc tgccatttct tcatccagtg ttggaagaat atgtggtgct
     6781 tgttcgatca ccatgacttc taaacctctt ctttttaggt tttctgccat ttccaatccg
     6841 atgaatcctg caccaacgat cacggcacgc tttggctgtt tttctaatgc atgcatcact
     6901 ttatcgatat ctggtacatt tctaatagaa aaaacattgt cggcttctgt tatccctgga
     6961 atcgatggaa caactggttt tgcaccagga gataaaacca atgcatcgta ctgttcaatg
     7021 tgtttttgac cctcatgtac gacttctacc gttttgtttt cgggaaagat ggctgtcact
     7081 tcatgatttg gacgcacatc taaacaaaaa cgagcggata atgattctgg cgtttggaca
     7141 agaagatttt cccgttcact gatttctccc gaaagataat aaggcaagcc gcaatttgca
     7201 aaagaaacat acggtccttt ttcaaaaaca acgatttctg catcttccat taatcggcga
     7261 agccgtgtag cagcagacat tccacctgct acacctccga caataacgat tttcatctta
     7321 tttccctcct actgttcgat caaaccattg attcatgcca ccacgtacat tcactacatc
     7381 ataaccagat ttctttaatt ctttagctgc ttgtttactc ctcatccctg attgacaaat
     7441 cacatagacg gtcttttctt tgtctccatc atatctgttt atactttgta atggaacatt
     7501 tttagctcct tttatatgcc cgccccggta ttctgcaggt gttctaacat caagtaacgc
     7561 tggattttcc aaaagctttt cctttaattc tgggactgtt atcgatttta ttgattgaaa
     7621 tagcaaattc attttctgca cctcatttac atttttataa atacctcatg gggtatatta
     7681 aacaggagct ttattaatat gtcaaccaga atgactcttc atttttttca ctacttttta
     7741 tatttgcata aaataccgat aaggggtatt atctaattat aaaaatagag gagaatgtgt
     7801 tatggagatt gataataaaa atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg
     7861 tggtatccaa aaaatgatcg aagacgaaaa agaatgtatg gatgtcatca ctcaattaag
     7921 cgctgttcgt tctagtattg atcgcgtaat gggcatgatc gttgcagata atctgaaaaa
     7981 ttgctttgag aaacctgata gtaatccaga agaacaagcg gctaaactag aacaagcgat
     8041 caagatgatt attaaaaagt aaaggaatga tttcaatgaa agtcgttttc catattgatg
     8101 aattagaaaa atggtcagaa acagggaaaa acgtaaaaaa cctgataaaa gcttctccag
     8161 aaacgacgat tgtcgtatct gtaaatggca tcgcgattac cggttatcta gactctgcca
     8221 atgcagaatt cttagatttg caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg
     8281 ctaatcatat ttcagaaagt tctttacctg aacaagtaat cgttgtacca gcaggtgtcc
     8341 ttgatttagt tgaactgcaa tcccaaggtt atgcttatat caagccttag tcagtagagg
     8401 ttgcgacaga agtgatcagc ttcaagaaat ttcggcttat ttttcgaaga agctacttct
     8461 ggaaaaccgt gaatcaaagg ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc
     8521 aaacaaaaat agcttctcat atttttcgtt tggttgggct tattaccgca gatgcaagct
     8581 tttgaacacc gtttaaggaa gaggttgtga aatcagcggt ttgacctaag tattagagaa
     8641 aaaaacggga aaatagctcc tcatattttg ccgttttttg agctaatacc gaaggtcagc
     8701 tgattgaaca ccgtttaagg aagagattgt gaaaaagtcg cttagtttcg agtattaaga
     8761 aaaattttgt gaaaatggat ttctacattt ttgcataaat ttggcttaat accgagaaac
     8821 tggcttttga ataccgttta aggaagaggt tgtaaaatca gcggtttgac ctgagtatta
     8881 gagaaacaaa cggaaaaata gctgctcata ttttgccgtt ttttgagcta ataccgaagg
     8941 tcagctgatt gaacaccgtt taccaagagg ttgtgagcct accatttaac tccgaatcac
     9001 aagaaataat ttcaaaaaca acttctcctg tttttggttc ggttggctta ttatcgagaa
     9061 gtagctactg ggagtctatt tatcaaaaaa aggaatgcga cataggtcac accccttttt
     9121 tattttcttt gaaacatctt tcggtactcg acagcataca agacaaaaga ttgtaaaaga
     9181 ccgtaagtga tgacggcaaa aatgactctc acccacagcg ttggtaattc tgggaaaagc
     9241 agcaaaagga tcatgccaac atagatgaca gcagtcgcta ctttgccata ccacttcgct
     9301 ccacttaatt tgatattttt agatagcccg attaatccag ctacaaacat aaagccttct
     9361 tttatcaaga atagaccaaa caacagccaa agccacggtc gataccatgc catagacaaa
     9421 atcagaaaaa gttgtgtcaa tttatctgca aacggatcaa gcaccttccc taagtctgtt
     9481 acctgattat atctccgagc aatgaccccg tctagataat cagacaaacc tgagacaaca
     9541 atgaccgctc ctgctatata aaatgactgt ctcagcacaa aataaagata aatcggcagc
     9601 aaaaaaatac ggaatatcga taaaagattc ggaatcgttt gtatttcttt acgccagtct
     9661 gtgcttaatt tcattttatc gccctacctc ctcagcactc ttttcttttt attttagctt
     9721 acacggggaa taaaaccaat gatttcactg tttttatttt tctttacaga cactgatatt
     9781 atgagctaat ttgagataat cacttgctgt tttcatcgtt tactcttctt tcttcctgtc
     9841 gtttgcgcat ttcttttact agtactttat gagctttttt tgatcctcgc tctaggtcac
     9901 gctgccgttt acgtgtttta taatcgataa acagactgtc taaatcgtaa cctgtcgggt
     9961 acagatcttc tgctgaacgc accagacgaa cacgctgacg aggcacgtgg attatttttt
    10021 tgtctaaata tacatcaatc gtatcttcac ctatatcttc ataaactaac gcttcttttt
    10081 ggtattcatt gacaaatatc cgatcacctt ttgcaaagag ctgacgttct tttttattct
    10141 ccttcctatt tgtaactgct gtcttaggaa gaaattcttt tttctgtgtc cgataggctt
    10201 tttccgccaa gtaacgctct gcttgatgga tcaatttcat cgacatatcc attttatgag
    10261 caatccacaa agcttggctt tctcctgttt cttccaaccc gcaactgata tttaggacga
    10321 agtgcttccc gatcgaaagc catagcagcc ggcacaaaat cttcatgatc tccggcgaat
    10381 ttcttgattt ctccataatg ggtcgtggca atgatcaatg ccccttgctc atacatagat
    10441 tccatgattg cgatcgctag tgcagcccct tcatttggct ctgttccact gccaatctca
    10501 tctaaaagta ccaacgtatt cctttttatc tttttcaaaa tagcagcgat gttttgcata
    10561 tgaccagaaa atgtactcaa cgcattttct aaattttggt ggtcaccgac atcgacgaaa
    10621 atctcatcaa agacagcaag ctctgtccct tcttttgccg gaacttgcat accgaacatc
    10681 gccatcaaag tcaataaacc gactgtcttc aacacgactg ttttgcctcc ggcatttgct
    10741 ccggtgataa tcaagcctcg atagtcttta ccgatttcaa catccaatgg taccgcatgc
    10801 tcaggtaaaa atggatgtct gccttgcttg ataatgatat gttccgattt attgattcta
    10861 ggtgtgcttc cattgatttc tcggctaaat tttgcccgag caaagatgat atctaacacc
    10921 gtgatcgttt cgatcaataa atcgatcact tcttcgtttt ctgcgatagc accagtcaat
    10981 gccgccagga cttgatattc ttcactgatt tcttcagctt ttaacaactg atagtgttca
    11041 ttcaatttag aaatcacagt tggttcaata aaaaccgtcg ttcttttatt tgattcatcg
    11101 ataatcgttc catctatttt atttttatag ctggctttga taggaatcgt atagtattcg
    11161 cctttttgaa caatcattgc ttcttggatc atttctttgt ttttcggatg acgcaagaat
    11221 ttcagtaatt ttgactggat ttctttttcg attagttgta attgtttgcg tactttacgc
    11281 aaattacgtg acgcgtcgtc actgacttta ttatttttta ttttctggtc aatcaactct
    11341 tctacattta tcaaatcagg aagatgtttg ctataagcaa acaacaaggg tgtctggtat
    11401 tgattcttat caaaaaactt tgtgatcatc cgactgcttc taagaaaatc cgcatattcg
    11461 atcaaatcgg ttggctgcag aatcaaccct tttttgactt gttctgtcag tgcatcgatc
    11521 ctcggcaacc ccataaatgg aacatgctga ctgctttcta agatcaagcg tgcttctctt
    11581 gtttcttctt gacgatccaa gacagtttgt aggtttgtcg agaccgtcat atcgctgatt
    11641 cgttttttgc tatagtttcc aattgctctt gcttggattt cctttttgat ccgatcaaat
    11701 tgtaattgac taatcatttc gttgtccata ttctctcctt ttcttgaaga gatacctgtc
    11761 ccaaaataaa taagggccta gagataaata aaaggataga acacagctta aaaaagtcat
    11821 gtcctatcct tattagaata caaagatatt gttttcctga caggtaccgt ttcataagca
    11881 caccaaataa actttgttta ttggctattc gacttactga ttagatacca gctacactca
    11941 caaaacatac cccattcttc taggtcttat ttattttcca cttaactata gcaagacaca
    12001 ttttggttgt caaactttag tatgttttat agatttacac cattaatgaa tagacaccta
    12061 ccgcgataat tgctccgatc attggacctg cgattggtac ccatgaatat gcccaatctg
    12121 aagttccttt attagctatt ggcaataccg catgtgctaa tcgaggacca aagtcccgag
    12181 cagggttgat tgcatatcct gttggccctc ctaatgaaag accgatggct aagatcaatc
    12241 cacctacagc gaatacgttc gttccaggtg caaattcgtt ttgtccaaaa gctagcaacc
    12301 caagaactaa tacaaatgta ccaattaatt ctgtgatcac atttgctgga taattccgta
    12361 tagcaggacc tgttgcaaac gttcctaaaa tcgcgctttc atctttcgtt gcattccaat
    12421 gtggcaaata agaaagccaa acaagaatgg cccctgcaaa agctcctaaa acttgcgcaa
    12481 tgatgaaagg aacgacaaga ttccaactaa ttgcccctgt catcgccata gctagagaga
    12541 ctgcaggatt caaatgagct gggcccatga atccagaaat atagacagcc attgttacag
    12601 cgagtcccca accaaatgcg atgacaatcc agccagaagc ctgtgcttta cttttgttta
    12661 aattcacagc cgcacacaca ccatctccaa gcaaaactag gatcagtgtc ccaataaact
    12721 cccccatgat ttgagtcata tcactgttca tttatttctc cccctttagt tcccgcagat
    12781 ctgattctgc aatcaatgct tctaaacgtt tagtctgttg cgccttctcc tcttctgtcc
    12841 attcaaaata tccagcaatt gcatcgatca caggttgctt gatctcatca agctgatctc
    12901 gttcaaacaa cagatgattt gtacgacgaa tgagataatc gcctggggcc aaaaccattt
    12961 cttcttctaa accataacgt aaccgagctg actcagcgag actcaaacca ggataaggtg
    13021 ccatttcttt tgctaattca aagatacgtc gggcattagt cccgtaaaaa tcagcgatat
    13081 aagtagcatc ttcttcctct aaaccggctg ctactccttc ttttgctaat tctgtcactg
    13141 tttcttctaa tttagttgga tcgaagtttc cacctgaaat ctgatatttt ttagagtcga
    13201 tttctttcgt ctctattccg tattcttctt ttaataattg gcgtatcaat cgcaaagctc
    13261 cttctgccat cttacggtaa tcggtgattt tcccaccaga taaagtcagc aaaccatctg
    13321 gttctctttc taaagaacta cctctggaaa tcgtagaagg tgctttttca tcacggctgt
    13381 tttccaaatg gttcaacacg tcttctactt cagcacgaga aactttgttt tccttatatt
    13441 cacttactgt atcgacaact ttattgaagc tcttgtctga aatcgatcca ttatctccac
    13501 cattgtaatc agaaccagaa ttaccaatca acagtggacg aagtcctgcc cagcttgctt
    13561 cgatatcatt caatgtgata tttgcttctg gatagcgatg attgatcaca tccaatagat
    13621 aatctacatc ttcttgtgtg actttcggat ccgtaaaatc tccttggtaa tccgtatctg
    13681 tcgtaccaaa gtaagtcttg ttttctcttg gaatagcaaa aaccatccgc ccatcttgct
    13741 ttcctgtatc gaagtatgtg ggttgcggta caggcagttt tttcgcatct acaactaaat
    13801 ggatcccttt agttggacgc attttaggag agactggacg cgtaaaattc aagtttcgta
    13861 ctttatctac ccaaggacca ctcgtattga tcactaattt tgctttgatt tcgatcactt
    13921 catctgtcag caggtcacga gctttgacgc caacgatttg atcgccatca tataaaaagc
    13981 caaccgcttt cattttactt actagataag ccccatcttc ggcagccttt ttgatattat
    14041 caatcactaa acgggcatca ttgttgcgga aatctagata gacacccgcg ccttttagcc
    14101 cttctttttt caaaaacggt tctctttcca atacttcttc tggtgtcagc gtataattct
    14161 catatttggt tcctgtcaca tttgccagtt tgtcataaag gtccattgct actttgaccg
    14221 agaacatatt gaaggttgtt gctccttcgc cctcatagat tggcaaaagc attggatctg
    14281 gttttgggat gtgtggggca atactttgta cgactgcacg ttcaccaact gtgtcagcta
    14341 ctacttccac atcaaatgtc ttcagataac gaataccgcc atgaacaagt ttggtcgagc
    14401 gagaggaagt cccttctgca aaatcctgca tttcgatcaa tcctgttttg attcctgatg
    14461 ctgctgactg gactgccaca ccggcaccag tgattccgcc accgatgatc aacagatcca
    14521 attcttcttg ctgcattttt tgaatgctat cttgtcttgt cttgtttgaa aacatatgga
    14581 tttcctcctt attcaaatgc ttgggtagct tttactgctt ttttccaacc gttgtaaagt
    14641 ttttctcggc gctcttcctc catttgaact tcaaatagct tgccagcttc ataaaattcg
    14701 cggatctcgt ctgtatcttt ccaataaccg acagctaatc ctgctaaaaa tgctgcccct
    14761 agcgccgttg tttccaagtt ttctgctcgt tggatcggta cattcaagat atctgtttgg
    14821 aattgcatca gatattcatt gtttgctgca ccgccatcta ctttcaatac aggaatcttg
    14881 attccagtgt catcttgcat cgtatcgatg atatctctca cttgataagc aatcgactgt
    14941 aaagtagctt taatgatatc ttcttttgtc gtaccacgtg tcaagccaaa catagcccct
    15001 cgtgctgcct gatcccaata aggagcacct agcccaacga atgcaggaac aacataaacc
    15061 tcatcttcgc ttgtagagcg tttcgctgcg tcttctgaat cgctagcttt ttgcaacatt
    15121 tgaagtccat ctctcagcca ttgaatcgaa gaaccagcca caaaaatact tccttctaaa
    15181 gcatagtaaa cctttccatt tatgccgtaa ccaattgtcg tcaataaatt atttttggat
    15241 aattgcggtt tttcaccggt attcatgaca ataaatgaac ctgtaccata agtattcttg
    15301 accatgcctg gttcgaatgc catttgtccg aataatgcgg cttgctggtc tcctgccatc
    15361 cctgaaattg gtgcttcgct tccataaaaa tgataacctt gcgtataacc ataaacttct
    15421 gagttagaag tgacttttgg taacatcact cgaggaatat ttagtaaatc taaaatctcc
    15481 tgatcccaat ctagctgatg gatattgaac aacatcgtcc gactggcatt cgagtaatca
    15541 gtaacatgtg cttgtccatc ggtcagtttc catactaacc aagaatcgat cgttccaaac
    15601 atcaattcgc ctttttcagc tcgttcttgc gcgccttcta catgatcaag aatccagcgt
    15661 atcttcgttg ctgagaaata ggcatcgatc actaaccctg ttttttcgtg gaaaaattct
    15721 tgatagcctt cattttttaa gttatctgca attccagctg actgtctgga ttgccaaaca
    15781 actgcatgat agattggacg tcctgtatct ttttcccata ttactgttgt ttcacgctgg
    15841 ttcgttatgc caatcccagc aatctgattt ggtttgatcc ctgattcgat gaacgctccg
    15901 gcaataactg actgtacaga attccagatt tcatttgcat catgttccac ccaactttct
    15961 tgcgggaaat attgcgtgaa ctctttttga gaactgccga tgtgtcttcc tttcttgtca
    16021 aagataattg ctcgtgagct agttgtacct tggtcaatcg ccatgatata cgttgattct
    16081 gtcattgtga ttccctccat tatatgcaaa cgctttcttt aactactctt atcatactac
    16141 tgttttaaaa aaagaaactg tcaaaatttt attctttttt tctttttttg acagtttttt
    16201 attatgaagc tattttttaa tcaattcttt tcttttttca gcgtctctat tcttcttttc
    16261 aatcgaataa tgtcgtaagg tgacgcaaat tctgacagat aatattcaca ctgttttttg
    16321 tttctctggc aaaatgtgat caccaaatca tattctttac cttcttcata acttccaatc
    16381 tcaataccgc cgattcccct cagttctctg atcagatatt gttgaaaagc aatacgataa
    16441 atcggcagac tttctaaatc gattccaatc actactggtt ttgcaatata aaaatcgatc
    16501 atcaaaagaa cattggcata ctggatcatc aggtaactaa gctcactatc ttcagctctt
    16561 ttcctcggca attgctgcac cgctgtttcc tgtaatttcg ccagtaactg tgttaaggaa
    16621 tgcccgagca ttttctgctg ctgttccaaa agccgatcac gctcatagac ttcgatcttt
    16681 ccaacaaaga aaaaccattc attgttgatc tgggaaattt ggtagcctac tgccttttct
    16741 tctttgattt tcaaacgacg aggacgataa tgccataaca tcgtttcccg aatgtaagta
    16801 tctaaaacag cggttggtaa ctttttgctc cgtgtcaaat catactgata aaatatttct
    16861 tcgtctacta tcgaaaaaga aacaaaaaag ctgtaaaaca tcattgtttc gtatacattc
    16921 agttctgctg ctgttcgact aagatgcatc acgatgattg gatcgattgc ttgataaagc
    16981 cggtccgact gatagagtaa tttcttttct ttcaaagttg cgtacgtact tttttcattc
    17041 aatagtcttt ttttcgtaat cccgagccag caagcaatct gtgcttctgc tgatgccgta
    17101 aacgttgttt ctaatacacg gtccaatcct agaatgaacg gtcttttttc tggtgtattt
    17161 tgcaaaaaaa gcggtcgctg atcttccggg atatattgga atagttcata ataaaaatat
    17221 cgtatctgta gctcttctcc gtataattga ccgtttttga tttgtaatcc aaattcctct
    17281 aagagctggt tcaattcttt gatctttcga aaaacagaag actcactgat attaaacgct
    17341 gtcgctaatc gaacgatgga tacttgctga tgttctaata aatagacgag catcttaaac
    17401 ttcaagcttt cctgcaataa aaacaataga attttttcca aacttacatc ttcgtccaac
    17461 acaagcagcg cctgatactc atttcgctgt acttctactt ttttttccat cattcggcca
    17521 atcaattcaa tatcttccaa atattgatca aacgaggctt ttgacagttg gacatgctct
    17581 ctcaaaccat tgatagaggc tgttccccct tgcagaacaa ggtttcgcaa aatcaggatc
    17641 tgtaactgct cttttctttc taataatgct tcaatcttca taggcagtat tcgccacctt
    17701 tgaccacacc atatctggca cacggtattc tggctggaaa aatagctcat gttcttctaa
    17761 ctgttcaaac gtccatcctt cttctactgc aattgtcagt tggtcaagaa ggtggaacat
    17821 ctcttttttg ctttggagct gtgctccaag aatctttttt tcagaatcaa atagaaattt
    17881 gagtttaact ttgctttgat ttattgctgt aagcttgtag tctacgtatc ctgtttcaat
    17941 tttttgggga taaaaaatgc tctcttcttc tgtcaaacct acgcttccta agaaatagcc
    18001 aaaccactga ttgccgactg gacgataggt tccctgatcc tttgtctgtg agccactaat
    18061 tgtcttgctg acatttcgtg ctgtacggac agcgttattt actagagaaa catacatttt
    18121 ttcatttgtt ggacgaaaag ttacttggat cgcatcgcca atcgcataaa cgtctttttg
    18181 agacgtttgc aagtagtcat ctacccagat cgttccatca tcattcaatg ttaattgttc
    18241 ttcccacatc tgattgtcgg gacgagaatg attagccagc aaaacaagat catagttctc
    18301 ctttcttttt tccgtctcta caatagcggt ttcattttcg gtcaaagaca caacttgttc
    18361 ttctaaaaaa agtgaaagcc cttgattttt catttccttt actaacggtt ctgtcatttc
    18421 aggatctaga taacgaaaaa gaattgactt tcggctttcg taaagatgga tttgtttttt
    18481 tcttgatgcc aacccttcag ctatttcgat tcctgcttgt cctgcaccaa taattgcaat
    18541 tttctttgca ttcatgatct tttgctgaag agccgttacg ttttgtatct ttttaactgg
    18601 gtgaataagc gttgattctg cagaaatatt tcgaaacttt tgatttgaac cagtcgctag
    18661 aatcaaacga tcaaaatcca atttttttcc tcctgctaaa acgacttttt tttctttcaa
    18721 tcctattact gtcttttctg tgtaaacatg gattcgatag cggtcagtca gttcttcttt
    18781 cgtgatccaa gaaagttgtt tttcactggt aacctttcct tttaatatca aattgaaccc
    18841 gctgggaatc catcctaact tcgattgctt ttcaatcaaa tatatttcac ttgttggcat
    18901 caattggcga agataaatag ccgcatgaat ccctgcaaac gacccgccga caattacaat
    18961 gttcatctgc attcctcttt cttataaggt gttctactgc taacttctca atcagaaaac
    19021 acagtattca taactcgctt tcaaccttat gatacatagc gatgcagcag aatcaaccct
    19081 ctccatcata ccgtgttcta ggtctgcttc tcaataatgt actagtggac ctaattgaag
    19141 agtatcattt ctgaagagat gtttcaaaaa atgtactctt ataaaagaaa aaaactcgcc
    19201 ttttacgtac ctctgtccaa ataggaaaca gaaaagaacg aaaagacgag ttttagcgtg
    19261 tgtaatgtaa tagttagaat agacacatca tttattttta attctttaat gcttcaacaa
    19321 ctgcttcgcc taattgatga gcagattgag gattttgccc tgtaagaagc cgcccatcaa
    19381 tttgaaccac ttctgaaaaa gctgctccag actgatagtt tgctcctttt tctttcaaag
    19441 catcttctgc taagaatggt acttcattcg ttgtaccatt tgcttcctct tcttcattac
    19501 tgaacccagt cactgtacgt ccagctaaaa tagattttcc agactcatct gtaactggca
    19561 acaatcctac aacaccgtga caaacagctg agaccagccc ttcatttcga tagatttgtg
    19621 aagcgacttt cccaagatct attgcttctg gaaaatccca cattgttccg tggccaccag
    19681 cataataaac cgctgcataa tcatttgcat ccacatcatc aggacgtaag gtatttccca
    19741 aggcccgatt tctgaaatct tcatcttcat aataggccca atcgatttca tccatattca
    19801 aaacactttg cggttctaaa ggtacatatc cgcctttagg actgacaaaa tctgcatcga
    19861 tcccattttt tactaataca tcataaaaat gagtcagttc acttaaccac aaacctgtag
    19921 gacgctcgat cttttcgtac ttttctacat ttgttaatac gatcaatact ttcattttcc
    19981 catcctcttt ctataaaaat tgcgttattg ataacgtgct ttcaagaaat tatataatgg
    20041 gcttggcaaa taaagctctt tatatgcttc ttcaaaaggt ttcgttacaa attcatcctc
    20101 acggttttcc aataatttct ctgcccagtc aggatcgacg atcatttgct gtccaattgc
    20161 tgcaagttct gcatatttca atacctgctc tacatcttcg cgattgcgga tcccaccgac
    20221 accaactaaa gggatctttc cttgaatcgt gttatggacc acttcaagaa tcgatttatc
    20281 gttgtattta tcagaacgag ctattcgatc atacatattt aatgaaacat gcagataatc
    20341 tagttttgac tcacgtaatc gttcaagaag ccagatcgtg tcttcaaaac ggatacctgg
    20401 tgtttcatat tcttctggtg agaaacgata ccctacgatg aatggttttt tagcatattg
    20461 ttcaaccgct gagaaaaccg ttgttaataa ttcttcaata aaacgataac gattttccaa
    20521 gctaccgccc cattgatcct ctcgtctatt cgaatgcgga gagaaaaatt gttggatcaa
    20581 atacgtattt gctccatgca actcgattcc atcaaatcct gattcaatcg ctctacgtgt
    20641 ggctttgcca aatgcttgga ttgtttcaac gatttcttct tcactcattg ctcgtggtgt
    20701 ttccgcatca ggacgttttg ctgcaactgc acttgcagat acaggctgtt ctccagataa
    20761 agttgctcga cttgtcattc gtccaccgtg gaaaatctga ataatcgctt ttgcgccttc
    20821 tccttggatt gctttcgcta gctcacttaa tcgaggaatc atttcatcat gagcgatgct
    20881 gagttcacct ggccagcctt ttccgatatc ttggacattt gcagcaccag taataacagc
    20941 accaagacct ttagaacgtt gtttataata agtgatctca tcttcggtga tcactccatt
    21001 gaaaaagctg agttgtgtcg tcataggcga cagcatcaat cggttcttca attgaactcc
    21061 tgaaggtaaa gtaattgatt gatcaaattg tgacattgtc attcctccca taattcgctc
    21121 tgattaacct atcgagaatc atctatcatt aaaccactcg attagttgta catgcaacta
    21181 atttgcttaa aaaattttat tttttttgta acttatctgt tgcatcattc attgtctggg
    21241 ctaattcttt acttgcttca tttcccaaaa ctgctttgta tgactgatga agttcttccc
    21301 agctaccatc gacttcctta gcaaattggc gtccttgttc tgtaagataa actaatgaca
    21361 agcgaccttc gctaactgtt ttgacgtatc ctttatattc taatttttca acaaaacgag
    21421 caatcgttga tggggcggaa tagagcattt gtgcaatatc tttttgtggt attccatctt
    21481 tttcttttaa tatcaataaa ataaaaccat aagttggtgc cagacctgtc cttctaaacg
    21541 cttcatcagc taatttattc aacatgcgat ctagcttttt cacgctaaag aaaagacatt
    21601 catctaacgc tttttcaatt ggcttttcca tttcatcccc tccattggcg tacatatcat
    21661 agtatgattt ttattttttg tcaaaaaaca cggaataatg tccgcctcct ctgtctagat
    21721 ttgaaaaaaa gaagtagcca tcttcaaaga atttcggctt gttttcgaca aaactccttc
    21781 tggaacaccg tgaatcagag gttgtgaaaa agtcgtttag ttttgagtat gaagaaaaat
    21841 tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga aaaactggct
    21901 tttgaacacc gtttatatca gaggttgtga aaaagccgtc ctgcatcaag taataagaac
    21961 agccaaacaa aaatagttcc tcatattttg tcgttttttg agctaatgcc gatggtcagc
    22021 tgattgtaca ccgtttattg agaggtcgtg agcctgccgt tgaactccga gtcacaagga
    22081 gcaatttcaa aaaacagctt ctcatgtttt atgactatgg tgacttgtga tcgaggagtt
    22141 ggcaggagaa tactgtttat tcgtaaagaa ggggctgtga caagactttt gtcacagtcc
    22201 cttatttcct tttattatag gatttcgcca tttgattgga tgacttgtct gtaccaatca
    22261 aatgattttt tctttaaccg tttctttgtg ccattccctt cgtcatcttg atcaacatag
    22321 atatatccat aacgttttga catttgtgaa gtagaagcgc tgacgatatc aatgcagccc
    22381 cacgacgtat aaccaaataa atctactcca tctttgatag cttccttcat ctgctcaata
    22441 tgctgcttca aatagtcaat gcggtaatcg tcttgaacgg ttccatcttc ttctactttg
    22501 tctatagctc cgagtccgtt ttcagctatg aacagtggtt tttgatacct atcatataat
    22561 tgattcaaag caattctcaa acccttagga tcaatctgcc agccccattc acttgatgga
    22621 agatacggat ttttaattcc tccagttatt aagtttcctt ggaccccttc ccaattttct
    22681 ggatgagcac tgactgcact agacatgtag tagctaaaag aaacaaaatc aacagggtag
    22741 gctgctaaga tttcttcatc acctacttct atttttaagt tgacaccttt ttcttcaaac
    22801 attcgttgcg tatacgctgg ataatatcct cttgcttgaa catcactaaa gaacaaagaa
    22861 gttctttgct gctcaattgt tttttgaata tcatccggat cacaagaata cggaacaaga
    22921 agctgatagc tgatcataca accgacttgt gaacctttga taatttcatg acaagctttt
    22981 gtagctaaag cacttgctat aaactgatgg tgtgcacctt ggtatttcgc ttgttccaaa
    23041 tggggatggt tttcttcaat tactcctacg ctaacaaaag ggtgatgttt tacacagttg
    23101 atctcattga aagtcatcca atattttact ttatttttat agcgtttgaa tactgtttca
    23161 gcgaaacgaa cgtaaaactg aataagcttt cgattaggcc atccaccata ttctgtaacc
    23221 aaatataacg gcatttcata atgggaaaga gaaacgactg gctcaatccc atattttaga
    23281 cattcgtcaa acacttgatc ataaaaaaat aatcccgctt cattgggtgt ttcttcatca
    23341 ccatgaggaa aaattcttgt ccatgcgata gacatccgat agcatttaaa acccatttca
    23401 gcaaataaac ggatgtcttc tttaaagtga tgatagaaat catttccgtg tcttttagga
    23461 taataatact tgctgttggg atcctgcgcc gcttttactt gctgatgcgt catcatatgc
    23521 cattgatctg tccatttttc tttaggtaaa tcgctgtcga atgtatacac atcagacaca
    23581 gataatcctt tttcatctac atcccatgca ccttcacatt gattggccgc tgtcgctccg
    23641 ccccataaaa atccttctgg aaattttttc atatcgatcc ccctaaactt gctagttttc
    23701 ttgtttgatt atttttctta tgactagtaa tgatattttt gaatctacta ctatttgtaa
    23761 caataaccat tgtggtcgga tcatatccct ctttgattaa ttcttgcaaa tctacttcca
    23821 tcaataaatc gcctttacgt actttttttc catcagatac tgcactttga aagtatttgc
    23881 catttaaatt cactgtatca ataccgatat ggatcagtat ctctgctcct tcttcactga
    23941 ctaccccaat tgcatgtttc gttggaaaaa ctgttgaaac ttccccgttt acagaactgt
    24001 agatttttcc gtccgttgga atgatcgcaa acccttctcc taaagatcca ctagcaaatg
    24061 ttggatcgtt tacttcagat aaatcaatta tttcacctgc tgcaggagta cttaacacta
    24121 tatcctttga ggattcttct tcgatttcat ccttgtagcc gaaaagaaaa gttagcatac
    24181 aggaactgat catcgctaag aaataactag caactagcca aacaaatcct tcaccaatat
    24241 aaatcgggaa agacaaaata cttcttgtag caactgccaa agcaccggct cctgcggcac
    24301 cagctactcc accaaatata gctccacata tacaagcaat gtaaaatggt cgtttatatt
    24361 taagagtcac accataaaca gcaggttcag tgattccaaa taatgctgtg ataaatgcag
    24421 ataaagataa actttttact ttcttatttt tcgttttcaa aaatacccca agtactgctc
    24481 ctgcttgcgc agctacggca gtcatacatg ctggacctag cgtatcacga ccgtagaaag
    24541 aaagattgtt ggtcattact ggtacaagtc cccaatgtaa cccaaaaatc actaacactt
    24601 gccaaaatcc gccgataaca gcaccagata aaacagggct aaagttataa acagctaaat
    24661 atccagctgc aatcagctga cttgcataat ctgcaattgg acctacaact aataaaataa
    24721 ttgggaccat cacgatgata caaatgaatg gtgtcaataa atttcgaata ctttcgtgca
    24781 agcttttgcg aatcttaggc tcaatataag aaagaaccca tacagctaaa attgctggaa
    24841 taaccgtact tgtatacctt gctagaatca cgggcatccc tagaaatcgc aaggttatgc
    24901 tatcactata tgcactgaca atcgtaggat aaacgagagc tgctgcaata accatagcga
    24961 tgaaacgatc tgtattgaac ttttttgcag cagtatatgc caaaaatata ggcaagaaat
    25021 agaaaaatgc atcagctgct gcattcaaaa tgatataagt tcctgattca gtagttaata
    25081 aattcaaact cgtacataag gcgagtactc ctttgatcaa accacttcct gccaagacgc
    25141 ctaaagtcgg ggcaaaaaca gcggcgatca tgtctataaa agagtttaaa atgccttttt
    25201 tctcagttcc tctctttcca ctagatgcat catcttctag tttcatttgt ttggataata
    25261 cgtcatacac ttcatttact gcatttccta ctacgacttg aaacattcct ccagcttcga
    25321 ctacagtgat cacaccttct agttgctcta ccttctcctt atctgccttc ttattatctt
    25381 ttagggtaaa gcgtaacctt gttgcacaat ggtaaacatt gctgacattt tcttcgcctc
    25441 cgacaaatcg gacaatatct ttcgctaatt gtgaatagtc catttttact tcccccttgt
    25501 taattaatgc cgtagcaatc cttcttacaa gataagtata gttcttagaa aacggtttct
    25561 ccattcaatt agtacccctt ctaatagcga acgaaactgt acaatagtgt gtatggttta
    25621 cttagccgag caaatcactc actgcttgaa agaaaacaag tatatcgttt ctcttatagt
    25681 caaaggagac aaaaaaaaca cgcttgatct tcctttgctg atgtataagt ggtatctcaa
    25741 gctgatactc tgtgattttt gtttctactt ggcttttgat aagacagcac aacaaagtct
    25801 cggcatttaa cggaagcata actaggtcag aactaggaga gattagttgc tgaatggaat
    25861 ctatcttttt atggattttt atttgatttt cttctttgta gtaatgatca agcagttttg
    25921 ttttcttttt ttcttcaact atggtcagct tttcacgcaa aatctgataa atctcccaag
    25981 attcaaactc atctagaaca ctaggtagat aaatcaaatc atcccactca aaaataagct
    26041 cttgctcagt agtcaaaatt gcatcatatt ccgttatttt gtttgattga tttttgaaaa
    26101 gatacactgg ttctatccgg cattctttga tccacatgtt catactttgt tgaatctgtt
    26161 tttttagtgt attcaaaata ctaattggct ggttactaac aatcagtatc tccccttgtt
    26221 ttcgaaaagg attcaacgct tcggcaagat acaaaacaaa ataagcgctt tcagctaaat
    26281 tgggacgtaa ttgaaaatgt attgggaaga aacgccgaat taaataaatt tctaaaggat
    26341 aacgggagat gatcttttgt acaaaatgat tcatggtatt atgtccggca tccatcctcg
    26401 ttttcatttg tttgatatgg gttagcaaaa attctgtttc atggaacaat aacgaaacag
    26461 gaagatctaa aaaacgaata atcgtttgct caaatgctct tagattcttc tctatttctt
    26521 tgtttttttc atcattaatc atcaaataat ttaactctaa tagtcgattt tcaattgctt
    26581 gtttttcttg attggaaaaa gaatagggta gttctgatga taactttttt gtgagggtct
    26641 ctaccatcgg aataaataat gtatacctgt ttgttttttc taaaagttct tctcttcgaa
    26701 gaatggtcaa aacgagatac cgagaaaatc gtatgaagtc ctctcctgaa acaatataat
    26761 tatatgatat aagaacctct tttaatgtct tttggacagt cggcatgata ttggaaaaaa
    26821 ttgtactgat ttgttgagga aaccttgcgt gttggatcac cgtttctgtt ccaacaagag
    26881 ataagaaaat tctttttgaa ttttcttcag caatgatttt tagtcctttt gtcgacgaaa
    26941 cttccagttc taatttagga ttgcgctcga tccaacgcaa aatcgttttt atttgtaatg
    27001 atacggctgt ttttgaaaga aagaaaatgt ctgctatctt ttgcatactg acataccctt
    27061 tttgaaacaa taagtacgtc acaatagttg aagctcggaa atccatgctg gaatagattt
    27121 tatgtttcgt cacctcttgt atgatttgct gtttagtttc agattcaatt gattctaaaa
    27181 aataacctga acgtttattt gactggataa tgattttctt ctcgtagata gcaagcatat
    27241 ttttgatttc agtttggatc gttttcttgt tggtacctaa agcttcagct aattcttcgc
    27301 ttgttatcca atgattctta tttcttaata ctacaagaat ttggagttgt cgtttattta
    27361 agtccatttg cttacacctc ctctttgtta taaacataca ggaaaaacct ctcaaatgac
    27421 aatagtcact tgcagaggtt taaaaatcat ttgaagaaag atctatattc aaggttttta
    27481 ccattagact ggataatttg tttataatat tcaaaactat cttttttcaa tctcctaaag
    27541 cttccgctcc catcattttg tctatccaca taaataaagc catatcgctt cctcatttcc
    27601 cctgttcctg ccgatacgat atcgattggt ccccaccata agtaaccaat gatttggcaa
    27661 ccatctttta ttgcctcagc aatttcctga agatgtattt ttagatactc acaacgtttt
    27721 ggatcgtgga ttcgattcaa ttcatctggt gcttcatcta gtccaatgcc attttcaact
    27781 atgaataatg gtaattcata acggtctgtc aattcattca tgacataacg aaaccctaga
    27841 ggatcaattg gccaactcca aggttcggga gatttttcct tcaaaaaggg attatcgaaa
    27901 ccatttgctc cgccagtatc tacgcgcatt tctgcttctt ttttatagac agcgctacga
    27961 taataactaa atgcaatata atctgccgtg ttagcttgca agcactgttc gtctccatct
    28021 aacattactg gtgcacagtc ttcttccttc catgtcctgt aaatataagc tggataatgc
    28081 cctcggcaca tgacatcgga gaaaaaccat gaattgcgtt taaattgcag tgaaccaaat
    28141 acattctctg gatcacagtc atatgcataa gtcgctaatg cagaaaagct acacataata
    28201 cccatcttag catttggatc gattttcttt aataattgag tagcacgggc gttcgctaga
    28261 aacatatgat gacaggcttg ataaatcact ttttttgtta gtgtagcaat gggttcaagt
    28321 ttatctctag cagggaatgt aaccaatcca gcagctgcat aaggcatacg aaaagcatta
    28381 ttgatctcat taaaggtcat ccaatatttc actttccctt ggtagcgttc aaagacggtc
    28441 tgcgcataaa attcaaaaaa gtcgattaat tttcgatttt gccacccccc atactctcct
    28501 accaatgcta agggggtttc ataatgacta agagtaatta ctggctcgat atcatatcga
    28561 tttaatgtat tgattagtct gtcataaaat acgagaccag cctcatttgg cttctgttcg
    28621 tctcctcttg gaaatattcg tgcccatgag atgcttgttc gaaaagcttt taaacctaac
    28681 tccttcagta aacgaatatc ctcttcaaat cgatgataaa aatcaatcgc ttcatgagaa
    28741 agataatgga gtgattcgtc taaagcaatc tcccaaatct ttttttcttc attccactga
    28801 atactaggat gctggttcat gatcccgata agcgtatctg tcacattagg taatttgccg
    28861 tcttccaacc atgcaccttc acattgatta gcagctactg ctccgcccca taaaaaattt
    28921 tctggaaata tttcctgatc cataactatc tctcctcgct atcaacaatt tcttacttga
    28981 agtatagctc gattcatcaa aacaagatat tctaactatt tcggcagaag agaagaaaaa
    29041 aattgaatgc tactcttcat ttttaatatt tatatataac aaatacataa gacgatcccc
    29101 gaaagaaaac actcgttaac catctaagca aacgatttat tttacttcca acacaaatgc
    29161 ttcataaggt cgtaaatcaa ctttgccgtc tggtaactgt ttcagaggat aatttgacac
    29221 aataaccctt aaagcttcac gtggaatagt gaacgtctgc ttttcactgg acatattaca
    29281 aataacgagc cattgctggt cttcatatat gcgagaatag gcaaagacat gctcaggcgt
    29341 cttttccact aattcatagt ctccccagac gatgatgtca tgttttctac gcaaatcgat
    29401 caatttcttg tacatataaa aaacagaatc ttgatctgct aacgcttctt ctgcattgat
    29461 ttctttataa tttggattga ctcgaagcca tggaattcca gtagtaaacc cagcatgttc
    29521 acgggcgttc cattgcatag gcgtcctagc attgtctctt cctttcgcct tgattgattc
    29581 caagatttcc tcttttgtaa atccagaaga aatcctttcg ttatacatat tgattgtctc
    29641 aatatctttt gcttccgcga tatcatcgat tgggcaattc gtcatgccta actcttctcc
    29701 ttgatagata taaggggtgc ctttcatcat atggagcaag atagcaaaca tcttggcact
    29761 ttcttttcga tacacatcgt catttcccca acgagaaaca attcgcggaa gatcgtgatt
    29821 attccaaaat aaactattcc atccttcatc acctaaagat gtttgccatt tggataatac
    29881 ttttttcagt tcagagatac tcaagggttt caaatcccat ttgtctttgc cttcttgctg
    29941 atctaatcct atgtgttcga attggaagac catggataat tcatttcttt taggattgga
    30001 ataaagttta gcgatttcgg tagttgctcc ccatgtttcg ccaacagtca tcagattttt
    30061 gtcaccaaaa gttgcttgat tcatttcctg aagatactca tgcagtttag gtccattact
    30121 tgtgatttct ttatccggtt ctttgccaat catatcgatc acgtccatac gaaagccccc
    30181 aataccttta tcgatccaga aattcatcat ttgataaatc tcttggcgaa cctgtttatt
    30241 tttccaattc aaatcaggtt gacgcttgct atacaagtgc agaaaatatt gccccgtttt
    30301 ttcatccaac tgccaagcag aaccactgaa tattgagcgc aatctattag gctcttttcc
    30361 attgaccgga tcacgccaaa tataatagtc ccgataagga ttttctttat ttttacgggc
    30421 ctcaatgaac cacggatgtt catcagaagt atgattcacc accagatcca tgatgatacg
    30481 gatatttctt ttctttgctt cttcaatcag ttcttccatt tcttccatcg taccgaattc
    30541 tgccatgatt gcttgataat tactgatatc atacccattg tcatcattcg gagactcgta
    30601 tactggagaa agccaaattg catcaatacc taaatacgcc agataatcca acctggaaat
    30661 aatcccccgc aaatctccaa taccatcttg atttgaatct tgaaaacttc ttggatagat
    30721 ttgataaaca actgaatggt gccaccattt ttgttccatg attttcctcc tataaatgga
    30781 tgtttctatt ttttgcttaa tcatttattt tagtctctct cactaaaaat tgtaacgctt
    30841 tcaaatagct attgcacctt gttatcggta acagaataaa acaaaaaaac agctgaggaa
    30901 attccctcaa ctgttttttg aaaggattga tttatgcagg gaagaatgct gatttcacaa
    30961 cttctttata aacggtgttc actcagctgt cccttggtat tagctcaaaa aacggcaaaa
    31021 tataagaaac tattttttcg tttttttctc taatactcag gccagaacgc tgatttcaca
    31081 acctctttat ttcacattca atatccacat gaatcccaca aatacaaaga acagaccata
    31141 catgattgga gatacttctt ttcctctttt agctgctaac atggtaattg gatagaagat
    31201 aaaacctaat gcaatcccat ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa
    31261 gaatgaaggg atcgcaatct ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat
    31321 caatactcct accataatca atgctggtgc agttacttgt gaagtaacaa ctgatagtaa
    31381 tggagaaaag aacaagccta acaagaagaa aatccctgta gtaatagctg tgatacctga
    31441 acgtcctcca acggcgatac cagctgatga ttctacaaat gcaccaacag gagaagtacc
    31501 aaataaggaa cccgcaagca tagctgttga atcagaagct aaagctttcc ctacacgagg
    31561 catcttgttg tctttcatga aacctgcttg gtttgctaat ccaactaaag ttcctgctgt
    31621 atcaaagaaa gtcacaagca agaatgtcag cactacgacc cacatttgaa gagaattgat
    31681 gtcccctaca tgttttaatg caactaaaaa tgttggtttc aaacttggtg ctgcggcaac
    31741 aatttcagaa ggcattggaa tcaaacctgt gaccagacct aaaatcgttg tagctgccat
    31801 cccgatgaag atgccacctg gtacacgccg aacaagcaag atagcagtta cgactaatcc
    31861 aaaaatcgtc aaccaagtag aaccaacgtt gagtgatcct aatcctacta atgtagattc
    31921 attcgaaacg atgatcccac cttcgctcag accaaggaaa gcgataaata gaccgatacc
    31981 gccagaaatc gcatatttca aatctgctgg gatcgcatca atgatcattt cccgcagctt
    32041 gaagattgtg atcaaaataa aaatcaaaga agcaacgaat acgccggcta atgctgtttc
    32101 ccatggaatc cccattccta aacaaacaga ataagcaaaa aaagcattga tcccaagagc
    32161 aggtgccgtt ccaattggat aacgggctaa tacccccatt aaaacacagc cgatcgcact
    32221 ggctaatgca gtcgcagtaa acactgctcc ttcatccatt ccagaagcac ctaatacagt
    32281 tggatttaca aataagatat aagccattga gataaatgtt gtaaatcctg ccagtacctc
    32341 acgttttacc gtggtgttca atttttctat ttcgaagtag gaaagtattt tttccttcat
    32401 cttccatccc cctaaatccg aacattataa ataaagtccg ttaaaatttc gatttattaa
    32461 taaaacaacg tgttttatca tatactaaaa caaaacataa gtaaacaaaa aattcggatt
    32521 ttaagcaaaa tccgaacgtt ccatctgatt aaatcccttt atctcaaagt tcatgaacat
    32581 tcactttgga ttacaagaat ttctttattg tgttaataaa gtttcttcat tctagtctca
    32641 aaaatcaata gtgtgatttg attttaccat ttcgtttctg ttcttctttg atgaattctt
    32701 ttgtatcgac aggattcaga ttatttagtc cgccaaaacc tgtatcgacc atttttcccc
    32761 agccttttgt cttctttttt cctgatttga gattctttgc tcgaagcttc tcactatgtt
    32821 ttgctcgccg atacttcaaa taaccttcta atcttcccat tagatccact gtttttcctc
    32881 ctttttattc aatcagacga ctcagctttt cctataacaa aataattgcc ttcagggtca
    32941 gaaaaattga aagtcaagat gccattgtta tctgtgattt ctcctggttc ttccatgttc
    33001 tcgtagatag tttcaaattc atccgtaaaa aatactaaag aaggcggtgg tcctaatacc
    33061 tctggtgaaa acttctgcac aaatactttt gggaatacgc ctaattctaa ttcttttgag
    33121 atgctgagta caatactctt aaattcttct ggcaattcga ttgtttcaga aatttctgca
    33181 ccaatttttt ctacaaagaa tcggcaaatc atctctacgt cattgacata aagcatcaca
    33241 cgtgtttttt gtatcatcat ttgacccttc tttctatttg attttctttc ctgtctataa
    33301 actgattcca ttatatcacg aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa
    33361 taaagcaata gtttctagga ttgctttcct ccacaaactt tttttacaaa aaaaaaacgg
    33421 caatgaatgc cgct
//
LOCUS       DAYYHX010000006        33309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000006 DAYYHX010000000
VERSION     DAYYHX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.006"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(106..381)
                     /locus_tag="ACHA6Y_000205"
     CDS             complement(106..381)
                     /locus_tag="ACHA6Y_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM8986332.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(624..1934)
                     /gene="der"
                     /locus_tag="ACHA6Y_000206"
     CDS             complement(624..1934)
                     /gene="der"
                     /locus_tag="ACHA6Y_000206"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM8986333.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2116..3345)
                     /gene="rpsA"
                     /locus_tag="ACHA6Y_000207"
     CDS             complement(2116..3345)
                     /gene="rpsA"
                     /locus_tag="ACHA6Y_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM8986334.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3480..4160)
                     /gene="cmk"
                     /locus_tag="ACHA6Y_000208"
     CDS             complement(3480..4160)
                     /gene="cmk"
                     /locus_tag="ACHA6Y_000208"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM8986335.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4209..4844)
                     /locus_tag="ACHA6Y_000209"
     CDS             complement(4209..4844)
                     /locus_tag="ACHA6Y_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM8986336.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4910..6313)
                     /locus_tag="ACHA6Y_000210"
     CDS             complement(4910..6313)
                     /locus_tag="ACHA6Y_000210"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM8986337.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(6300..7331)
                     /locus_tag="ACHA6Y_000211"
     CDS             complement(6300..7331)
                     /locus_tag="ACHA6Y_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8986338.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7362..7583
                     /locus_tag="ACHA6Y_000212"
     CDS             7362..7583
                     /locus_tag="ACHA6Y_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM8986339.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7748..8485)
                     /locus_tag="ACHA6Y_000213"
     CDS             complement(7748..8485)
                     /locus_tag="ACHA6Y_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM8986340.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8638..9978)
                     /locus_tag="ACHA6Y_000214"
     CDS             complement(8638..9978)
                     /locus_tag="ACHA6Y_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8986341.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10215..11738)
                     /locus_tag="ACHA6Y_000215"
     CDS             complement(10215..11738)
                     /locus_tag="ACHA6Y_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8986342.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12116..12727)
                     /locus_tag="ACHA6Y_000216"
     CDS             complement(12116..12727)
                     /locus_tag="ACHA6Y_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8986343.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12850..12975)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13057..13773)
                     /locus_tag="ACHA6Y_000217"
     CDS             complement(13057..13773)
                     /locus_tag="ACHA6Y_000217"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8986344.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13779..14348)
                     /gene="scpB"
                     /locus_tag="ACHA6Y_000218"
     CDS             complement(13779..14348)
                     /gene="scpB"
                     /locus_tag="ACHA6Y_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM8986345.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14332..15102)
                     /locus_tag="ACHA6Y_000219"
     CDS             complement(14332..15102)
                     /locus_tag="ACHA6Y_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM8986346.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15099..15470)
                     /locus_tag="ACHA6Y_000220"
     CDS             complement(15099..15470)
                     /locus_tag="ACHA6Y_000220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8986347.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15489..16376)
                     /gene="xerD"
                     /locus_tag="ACHA6Y_000221"
     CDS             complement(15489..16376)
                     /gene="xerD"
                     /locus_tag="ACHA6Y_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM8986348.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(16472..16945)
                     /locus_tag="ACHA6Y_000222"
     CDS             complement(16472..16945)
                     /locus_tag="ACHA6Y_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8986349.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(17050..17913)
                     /locus_tag="ACHA6Y_000223"
     CDS             complement(17050..17913)
                     /locus_tag="ACHA6Y_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM8986350.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(18109..20208)
                     /locus_tag="ACHA6Y_000224"
     CDS             complement(18109..20208)
                     /locus_tag="ACHA6Y_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM8986351.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(20428..21537)
                     /gene="rpoD"
                     /locus_tag="ACHA6Y_000225"
     CDS             complement(20428..21537)
                     /gene="rpoD"
                     /locus_tag="ACHA6Y_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM8986352.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(21561..23402)
                     /gene="dnaG"
                     /locus_tag="ACHA6Y_000226"
     CDS             complement(21561..23402)
                     /gene="dnaG"
                     /locus_tag="ACHA6Y_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM8986353.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(23584..25899)
                     /locus_tag="ACHA6Y_000227"
     CDS             complement(23584..25899)
                     /locus_tag="ACHA6Y_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8986354.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(26084..26944)
                     /locus_tag="ACHA6Y_000228"
     CDS             complement(26084..26944)
                     /locus_tag="ACHA6Y_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM8986355.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(27003..27635)
                     /locus_tag="ACHA6Y_000229"
                     /pseudo
     CDS             complement(27003..27635)
                     /locus_tag="ACHA6Y_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            27802..28563
                     /locus_tag="ACHA6Y_000230"
     CDS             27802..28563
                     /locus_tag="ACHA6Y_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8986356.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            28589..31180
                     /gene="mprF"
                     /locus_tag="ACHA6Y_000231"
     CDS             28589..31180
                     /gene="mprF"
                     /locus_tag="ACHA6Y_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8986357.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(31368..32555)
                     /gene="tuf"
                     /locus_tag="ACHA6Y_000232"
     CDS             complement(31368..32555)
                     /gene="tuf"
                     /locus_tag="ACHA6Y_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8986358.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            32923..33279
                     /locus_tag="ACHA6Y_000233"
     CDS             32923..33279
                     /locus_tag="ACHA6Y_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8986359.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         9249 a         7065 c         5740 g        11255 t
ORIGIN      
        1 gacatacgga ttcggaatct ctgtctctta tttattcctt ttctgctagg aaataaaaag
       61 aaaagcactt gtagccctag agccacaagc gctttttcaa ctgtcttatt taacagcgtc
      121 ttttaatgct ttacctggtt tgaatgcagg tactttgcta gctggaattt cgatttcttc
      181 accagtttgt gggttacgac ctttgcgagc tgcgcgttcg cgaacttcaa agttaccaaa
      241 accgattaat tgaacttttt cgccgttagc taaagcatct tgaatagttg aaaatacagc
      301 atctactgct gcagttgcat cttttttagt taaatcagta gctgaagcaa ctttttcgat
      361 taattctgct ttgtttgcca tggatagatt cacctcctcc acaagagata tgatagaaat
      421 atcatttagt taaaactttc tgagtggtcc agaagtttaa aaagaaatat tgattaactt
      481 caatattctg ttatcacgat atcatatgaa ccgcttaata gcaaggtttt taccaatttt
      541 cacatgcttt ttttagtcat agaaacgtaa caaacgaaac tgtctttttc ttttgaaaaa
      601 aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag ggataattcg gatggacgtc
      661 ccttcaaaag taaatgcttt tctgatttga ttttctaaga aacgttcata agaaaaatgc
      721 atcaattcct cttcattgac aaaaacgaca aatgtaggtg gtttgacagc gacttgtgta
      781 gcataaaaaa tcttcaggcg ttttccttta tctgttggtg ttggattgat tgctacagca
      841 tccataatga tatcattcaa cacagccgaa gagatacgaa gattctggtt catacttact
      901 tgttcgatca tttcaggcag acgttccaac cgttgtttag tcactgcaga aacaaaaacg
      961 attggagcgt aatcaagata ccggaattca tcacgaatct cttgttcgaa atcacgcatt
     1021 gtattggttt ctttctttac aagatcccat ttattcacga cgataataat tccacgacca
     1081 gcttcatggg cgtaacccgc tactcgcttg tcttgttccc gaatcccttc ttcagcattc
     1141 agtaccatca aaacgatatc agaacggtca atggcacgca ttgcacgcat cacgctgtat
     1201 ttttcagtat tttcgtatac ttttccgcgt ttacgcatac cagctgtatc tatcattaag
     1261 aatttctgac cactttcaga aacgaagtgg gtgtcgattg cgtctcgggt cgttccttca
     1321 atatccgaaa caattacacg ttcttctcct aaaatcgcat tgatcaaaga ggatttacca
     1381 acatttgggc gaccaatcaa actaaactta atcgtgtcgt cgtcctcttc ttcagtctct
     1441 tcagagaaat acttcactgc ttcatctaaa atatcaccaa tccctagtcc atggcttcct
     1501 gaaacaggat aaggatcgcc tagccctaag gaataaaatt catagatatc gtttctcatt
     1561 tctggattat ctactttatt gactgctaaa atcactggtt tattacttct gtataaaatt
     1621 ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc cgctgactac acaaatgatt
     1681 acatctgctt cttcaatcgc gatttctgct tgatgcttga tctgatccat aaaaggttca
     1741 tcgccaagat caattccgcc tgtgtcgatc acgctgaatt ctcgacctaa ccattcacct
     1801 tttgcataaa tacgatcacg agttactcca ggagtatctt cgacaatcga gatacgctca
     1861 ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg ggcggccaac gattgcaatt
     1921 gttggatttg ccatattatt tcctcctcac aagtttccaa tctcacttag tttaacgaat
     1981 gcattgccaa ctttcaagca tagattgtca attaaacgaa gtatttttat tccttattga
     2041 aatactaatc gcttttcaaa agaaaaactg agacaaaagc agctgctttc atctcagtca
     2101 cagatacaaa atcaattatt tttcagaatc atcggttgct ttttcttcta cttcttcttt
     2161 caacgcatca cctaaaatgt ctcccatagt aaatccagta ttttcttctg gtaattcata
     2221 agactcaaca ttttttggtt cttcttctga agctggtttt tcttctaaag ctttgatact
     2281 caatgcaata cgatgttctt ctggatggat ctctaatact ttcactttca cttcgtcacc
     2341 ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt gaaatttgag agatatggac
     2401 tagcccttct actccaggga acacttcaac aaaggcaccg aagcttgtca aacgtttaac
     2461 tgttccgtct aatactgtgc caactggagc ttttgtatcg atttctgacc aaggtccagg
     2521 ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca ggattgatag acaacacttt
     2581 gacatttaca tcatcattga cagccaatac atcacttggt tttgctacat gactatgtga
     2641 aatctctgag acatggacta gaccgtcaat tccgcctagg tcgacaaatg cgccaaaatc
     2701 agtaagacgt gcaacacgtc cactgagaac atctccctca tggatagttg aaagaatttc
     2761 ttttttcttt tcttctcttt cgttttccac taccgcttta tgcgataaga tcaaacgatt
     2821 ttctgatggt tcgatttcga tgattttaaa gctcaatgtt ttgcctttat agtctgaaaa
     2881 gtctgcaaca aaatgatctt ccaccattga tgcagggaca aaaccacgta caccgacgtc
     2941 aacgactaaa ccgcctttga caacatttgt aacaggtgct tcgatgacct tgccctcttg
     3001 gaaatctctt tcgatatctt cccaaacttt tttagcatct aaacggcgtt ttgaaagtag
     3061 atagctgccg ttttctttat ctttgccgat cgtactgata acgactaaat ctaatacatc
     3121 accgactttt actacttcgc tgatatcttc taccggcaaa gttgataatt cttttgccgg
     3181 aacgactcct tcaactccag ccccttcgat tccgacaatc acttgtttgt cttcaatgac
     3241 taaaacttcg cccttaacaa catcgccaac ttttacttct tgtacgcttt tcattgcgtc
     3301 ttccattgac tggttgttct cttgattttg atcaaattct gtcataacac ttgtcctcct
     3361 aaccaacatt ctgccgtaag tacggtactg tttttagttt aattgattat tcaaaaaaaa
     3421 taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt tttcattttt tttcttaatt
     3481 taaaaaagaa gataaccttt tttcaaaaca acagctttta ttgctgcaac gacttctgga
     3541 atcgatttgc cagtcgtatc gatacgaaca gcatcctctg cttgcttcag cggagagacg
     3601 tcccttgttg aatcataatg gtctctttcc tgtatctctt tcgtcagtgt ttcaagatca
     3661 gtcggaattc ctttttcttg gttctctttg aaacgtcttt ctgcacgctc acttacgctt
     3721 gctactaaaa agattttcac ttctgcattc ggtaatacag ctgtcccgat gtctcttccg
     3781 tccatgacaa cgccaccgcg ttcccctatt tttcgttgga cagcaactaa ttcttctctg
     3841 acttttccat gtgcagagac ttcagaaaca gcttttgtga catctggttg acggatttct
     3901 aaagtcacat cttttgatgc tatgaataca tgctgtccat cttcttgttg cgaaaaagag
     3961 ataggatact ttttgatcag cgaaactagc ccttcttcat ctgaaaaaga tacattgttc
     4021 gtaatagcta aataagttac tgcgcgatac atggcaccgg tatctgtata aatataattc
     4081 aattcttttg ctaatatctt ggcaacggta cttttcccag atgaagcagg cccgtcgatt
     4141 gcaatactga tttgtgtcat gatataactc ctttttctca gaaaaaaagc cgcaaagcgc
     4201 ggctattatt atttaattct taattcttga ccagggtaaa gcataaagtt gtttggatct
     4261 aagccattca attcaaacaa ttgatcagtg gtgatccctg cacgctcagc cacttgtttt
     4321 ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg cagctgatga agaaggtgtt
     4381 gtttctgctg cattgctgct tgaatcccct gcttgataag ttgcctcact tgaggaagtc
     4441 ggtgttgtct ctgctgcaat actgctttct gacgttacac ctgtatcttg agacacagta
     4501 gattcttccg cgattgtact agattctact gttgaagtct cagttgactc ctcttttgtt
     4561 gtacttgtag aactttctac tactgatgtc gaagtttcag aagaagttgc tgctgttgca
     4621 ttgttgcttg atccatttct gatccagaaa taagcacctg ctggaatagc gatacataag
     4681 accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc ctttcttttg ttgtctctga
     4741 gaacttcttg atgaaacttc atcatctgta tcgtagattg gttgttccca tggttcttgt
     4801 gtttcgttat tttctgtaga ttgatgtcta tcttttctgc tcactgtttt acctcctaaa
     4861 ctctatcgtg tgtattatac cataggaaaa agtgtcttgt cctaaaaaat caatgttcca
     4921 tgtttttttt aaataaattt agtaaaatcg ttccccaatg aagttgttca gtcttagaaa
     4981 aaagcttcct ctttggcaga agttctgctt ttgctccatc aatatcacaa caattctgcg
     5041 gcttttcgga gagtttttcg ccaaaataag ctaagatgaa ttttctgcgg cagttttctt
     5101 cattaatata gcgaagcatc tgctgcaggc ggaattcttt ttctttttcc tgacgtttga
     5161 gccgttccac ccaattttct ggtttttcag actgctggat cagctccatc catttttttt
     5221 gcagctcatc aaagggagct tgctctgtgg catattccaa ataaagctca aaactttgtc
     5281 gctgttctct agaaagttga ttaaagaaat aatggatacg ttcatcccca ctttgataaa
     5341 aaagaattgc tgcactttct tcctgatctc tgcctgctcg accgatttct tgtacatagt
     5401 tttctagact gtctggcaaa tcataatgga ccacataacg gatatcagat ttatcgatcc
     5461 ccataccaaa tgcattcgta gcaatgagaa actgcagctg attttttaca aactgctgtt
     5521 gaagttgtct tctttgagct gcatcaagac caccgtgata gtatccaact gtaaaacgtt
     5581 ccctgaataa atgatacaat cgttcgactt ctttttttgt tgcacaataa atgattgccg
     5641 ctccttgtgc tctttccatg aagtgttcaa gttcttgttc tttttgctgc gttttccgaa
     5701 caaaaagcga gatattttgc cgattgactg attgtctgat ctcttttggt ggtcgatcaa
     5761 atagtacatg acgtatatct cttgctacta aatctgttgc tgtcgccgtt agtgccagtg
     5821 tgaccgggtt tcctaaaagc tttcggattt ttcctaactg ttgatattct ggacggaaat
     5881 caactcccca ttgagacacg caatgagcct catctactac atataacgca atattttgtt
     5941 ttttcagatg ctctaaaaga gaaggattag tcaacatttc gggactcaaa aacaaaaatt
     6001 tatattgcga tagatgacgg aggacgtatc ttctttcaga ttgcgaaagt aaactattga
     6061 aagcagctac tcgtttctcc cctcgttttt gtaattgcgt cacttgatct tccattagag
     6121 aaagcagcgg tgacacgatg atgaccagac cttccattaa ataccccgtc aattgatagc
     6181 aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt atcttctcca tcaagcaacg
     6241 cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa tccaaaatat ttttttaatt
     6301 catttattaa cattctgtat cgcttccttt ctcaatagat agatttgata caaacgaaat
     6361 tctccataat ctaattgtcc atctacattc cattcgctaa agcgccactc tcgcacggaa
     6421 tccttatttg gttccaaacg ttcgattgtt tctgcagaga gaatccactc gtatggaaaa
     6481 tcatcaaaaa acaaggccca ctcaataaga tgatctgtta ctgttcctct ttttaaatga
     6541 cggattgcca tgatctggtc aattgtctga ccattcataa acatctgctt ggtgatcatc
     6601 atgctttgat tgaaattttg ctgtaataat gaatcgatca aagcgtgcca aagcgaatct
     6661 ggccgttttt cgatttgtgc taacaaaagg tggatacatc tgctttgttg cagataaacc
     6721 gctgactcat cattgttttt tgccagctga taaggaagca gccctgttcg ctcatttcca
     6781 gataattggt tagctaaaaa atctgctgct tcttctggta tctccgaaaa cagctgcacc
     6841 aaactctcat acgcagtatc aatcaaaatt tcacgtggca attggctttc actaagccat
     6901 ttttttagtt ggaaagtata aaaaggactt gtctctgctg gaagatagtc ttgaataccc
     6961 gttgccagat tagaaattac ttgaacagca aatttaatga gccgccaact agtttcccct
     7021 gttctgccat agcggtcaaa acgcaaacca gtatgttcta tttgatgttc ggacaacata
     7081 tcagcccctg caggcgttag ctttgcttca ttttcattta tttcgatcca accttgctgc
     7141 gcaatttttt gcaaaatttg ataaaactta tcctgcttca attctggaaa actgttatgg
     7201 ataaacaaaa gttcgtgggt aaatccataa ataagtactg aagtcgttcg ttttcccaca
     7261 aggagttggt aaagagaact aatccgcagc ttgtgtcctg atgaaaatag cgataaaata
     7321 aaaacaatca tattattctc cttaagttag gatggttaga aatgtcacta ctatgtaaac
     7381 ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat cgaagcgcca gatattttcg
     7441 attatgatga agaaggaatc gttttatttg cccaagaacc agaggctcat caacagttcg
     7501 tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca aaaatgtcca gttcgagcga
     7561 ttttattaga aaataaaata taaatataaa cgaaacttta aaaacgtaca ctaagttctc
     7621 taaacttgaa aataagccgg gatctgtttc tcttgttccg tgaaatcccg acttattttg
     7681 aaagattcat ttgctatcta tagttaattg actagttttt tcacggaccc tataggaccc
     7741 atttatatta tttcaacttt ttcagccagt tttccaatgt attgacgatc acagtttgct
     7801 ggtctgcgtt agaaatttct gcctctccat ctcctttttg ctcaccatag ctgccaaaac
     7861 ctccatgatt tccgccattg attgatacat actctgttct ttccggtaaa tactgcttag
     7921 cttcttgata agcttcctga ttcaatactt tatcttttgt cgccgtgatc gataaaacag
     7981 gaacagctga ttctgtcaat gttccttttt tgtcgggata acttgccatg aagaataccc
     8041 cttttaaatg tttgtctgaa tgattcttat tgacataacg acttgccatt acgccaccta
     8101 atgaatggcc accgatcaca taatcggaat ttggaaaatc ttctttgacc ttatccgctt
     8161 tgtttccgcc catcactgct agatcaagtg gcattttgac aatagcgact gaaaagccgt
     8221 tttttgctaa atcttgcgcc caaatactat aactttctgg ctctacgaga gcaccaggat
     8281 aaaaaataag taatggagac tttccatgat tctttttctc tccttcaaaa aataagtaat
     8341 cttctttgtt ttttccttct tttaccaaca cttgcgcttc tgacgatggt tcgtacgtat
     8401 tttgctgtat atagtaataa ccggctccac cgagtaatcc taaaatgatg actaagccaa
     8461 ttagtattcg tctaaatatt ttcactttct cccacctttt ctatctgagt gacccctttt
     8521 tcttaatcaa gtatgattat accttaaaaa aataagagag aaatgcaaac tttccagcat
     8581 caaataaaaa aagacggaat atccatttga atatccgccc tttttgtatg tttattttta
     8641 tttagcacat aaacgataca cagcttctgc ataaatttcc attgctttat acatactact
     8701 taattcccat cgttcattcg cctggtgcat aaagtcagga gtatcaggga acatcgcacc
     8761 gaatgccaca cactggttca tcgtacgagc aaaagtagcg ccgcctgata caaacggttc
     8821 agtcatgtct ccagtaatat cgcgatatgt ccccaataga tttttcacta actcactgtc
     8881 tagtggcacg tataatgaat caaggaagtc aaattcttca taagtcaacc catatttttc
     8941 tgctgtttta gttaatttag ctactaaatc atctttcttg aatgtaacag ggattctcat
     9001 atctacaccg atttttgttt gttctggtgt gatttccaaa cttgcaaagt tcatcgttag
     9061 ctcgccggat tgttcatctt ctgttttgcc cacgacagct tcaccagttg cattttcttg
     9121 aactaatttt cctaagaaat tgattgggcc aaaatcaaat acttctgaaa gagcaattgc
     9181 cagacgagaa atagcattca ctccttgtgc tgcatctttc gcgtggatac ttttaccaag
     9241 aacgacgatg ccttcacctt gttcttcgaa atcgaattcg tgttttttca atgcttcttt
     9301 tacttcagaa agcttttctc cagagtaagg agctgcatca ggaacaacat tcaacgctcc
     9361 tccagctttt acagaaaact cgttcgttcc tggtcctgtc aagtaagctt gcaataagcc
     9421 tttttctgca taaattaaag gaaattcagc atctggcgca aatccttgcg taattccttc
     9481 ttctttttcg ttatattttt ctaaacaacg ccataaattt tcttcatctg taccaaagat
     9541 aaaacgaata cgtgtcttga attccacgcc agcatcaatc aaagctttga cggcatacat
     9601 tgccgcaata gaaggacctt tgtcatcttg ggatccgcga ccaactaacc agccgtcttt
     9661 tatagttgga tcgaatggtt ttgtttccca attattttcg tcccctgctg gaacaacgtc
     9721 catatgacac aagataccga ataattcatc tccagagcca atctctgaat aaccgtaata
     9781 gccttctgga tctttaaatg ttctaaatcc tagcttttca ctgatttcca atactttatc
     9841 caaagcttca atcacttttt taccgaaggg atgtccttgt cctgaatctg cttcatctag
     9901 tacggaagga attcggatca attcgctcaa tgattctaat gcttgttcat gatgttgttc
     9961 tgtcacaaat tttttcatga tattatcctc ctaatatgtt aaaaagggtc atgtcaaatt
    10021 cctccgacac aacctcttct tctcaaataa tgacgtttta agcctgctcc atcagcaagc
    10081 agaatgttct taacaaatga tgaaagaaaa aacactcact gaatggagct attgacttat
    10141 ccacgtgctt atcgatagcc gtttatcggt ttcttcattc ttatgataga ccttgtacct
    10201 gtatttgtct aggtttagaa cattgctgct acacctagga tcacacagat ggctacaaat
    10261 aatatcgcaa ttaatttacc ggtaaatttc caccacactg aaacatcgaa acgagcaatt
    10321 gctacagcac ccattacgat agcagatgtt ggtgtaatta agtttactaa accagatgca
    10381 gattgataag cagtgattac taaatcctta ccgacgccgg caaattcacc cataggtccc
    10441 atgattccca ttgttgcagc agctaagccg gaagttgaag gaatcaagaa tgacataggg
    10501 atatagaaaa taaatgtaat aatgatgaat acacttgaag ataatgaact taatccttgt
    10561 tcgccccaat gtaagatggt gtctgtgatt aatccattgt tcataaccac ttggatacca
    10621 cgagcaacag ctactacgat tgctacaccg atcaaatcag aagcaccagc taaaaattca
    10681 gagacaaatt cagactcttt catgccataa gtgattgcta ctacgatgga cattactaag
    10741 aataacatag taatttctgg gaagtaccag tcacctaatg gaagcatgct tttacctaga
    10801 accgtaccta gtaccggaag gtttgttaac cagtttgtca atgattcaaa aatcgtgaaa
    10861 ttcgcattca aggttgtcca aggaatcaaa ctgatgatca tgataccaaa agtaataaag
    10921 aacaatacat taacatgttt ttgttttttc gtgattactt cttgacgacc gattgcttca
    10981 atatcgaaat gcttcatatc ttctttacgt tggttgtata ctaatgattt tgttggatct
    11041 ttttcgatct ttgatgcata acgatacaca tatacaatag aaatagctaa caaaataacg
    11101 aacatcaaga atcgaagacc aataccttca cccatcgaaa tcccaacagc ttgtgaagct
    11161 acgcctgttg caaaaggatt taccgttgaa gctagacagc ctacttgaga ccctactaat
    11221 acgattgcta cagctactac tgtatcaaag cctacagcca tcatgactgg gatcagtaaa
    11281 gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag agccgcctaa tgcaaataac
    11341 agcatcaata ctggaataag cattttttct tttcctttga atttcttcac aactgaagca
    11401 atcccggcat ctagtgcccc tgttttgttg ataataccta aaaatccacc aacaaccaaa
    11461 atgaagaaag agattgggat tgcagcaggt gtttctttag ttagctcccc cgctggtaca
    11521 cctagcatcc cattgattgg cgccatgaaa atatcccaca gcccttgagg attttgttgt
    11581 actcgttcat atgatccagc gacgatgttg cctgcatcgt ctacactata gtgcccagct
    11641 ggtataaacc aagtaagtac agcgattact gcaataataa taaataagac cgtatacgct
    11701 gaaggcattt gaaatttttt cttttttgtt tgttccatct tcttcttcct ttcttttccg
    11761 ttagagacaa caattttggc tctaatgtta ttttatcaaa aagagtatca gaacaaaaat
    11821 aataacacgc attttgtaaa acttttgttc aaaaagttcg tttttcccta ttccctcctc
    11881 ttttttcagg tttttaaagc tttcttcttt ttcattgtag cgtttccttt tttttataaa
    11941 caaacatttt cgtcttttaa aaggagaaga atttcacatt tgcaaagaat tgattgattt
    12001 ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa aacgtttaca ttattgcatg
    12061 tcttgtattt ttttccaaaa aaaaagacca agaaatttct tgatcttttt gatatttatt
    12121 tgactgctgt atgacgttgt tcgaactgat gttgttttcg acttaaccat ggaagaagtt
    12181 gcgcatgcag cactaaaaag acagcactca cgatgaatcc tttgatcaag ttgaaaggaa
    12241 cgatcccaat cagtacatag tttgctaaag acatgcccag catctgtgtt gcgttcagcc
    12301 ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt tgcgatactc ataaaaatag
    12361 ttagagcgat ggttcctgta atgacaccta aaaatttgtt tcttccttta tgctgtttct
    12421 ttacactaaa gaaataataa atcgggaatg taaagatcgt tgttgataag aaactagcag
    12481 tgtctcctac caaattatcc ggagaaaaac ctgtagtcag cagatgtaat actgaacgca
    12541 agaacgctgt tgcaactccg gccgctggac cgaataagaa catgctgatc aataccgaga
    12601 tatcactaaa atcgatcttc aaaaaactaa aagccggcat gatcggaaaa gcaaaatact
    12661 gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac cattttctgt acacgggtgt
    12721 tcttcatttg aaatcctcct gttaggactc ctcttcagcg aactttttga cgtttctctc
    12781 caaatgaaaa aactctattt tccagggaaa atagagttag agttatttga tcaagcgtcg
    12841 tcaaataagc tctatcttct ttatccagac tatactgtcg gtatcggaat ttcaccgatt
    12901 cagctatttt tttgcaaaat aggtcgtgga ctataaccac cggtcgggaa tttcaccctg
    12961 cccctgaaga cgaaccttat ataatttttg tttacatatt cattatacat atctattcaa
    13021 aaaacgcaac atctaacact tataaaaagt aagtgttcat tttgagtggt tcagcaaact
    13081 gctgacttct tttttgttca gttcacggta atccccagga cgtaggcctt gtaaagtcag
    13141 atctccgtat ttttcacgtt ttagtttttg cacaggaaga ccaactgctt gaagcatatt
    13201 tttcacttga tgattacgtc cttcgtggat cgtcaattcc acgatactgt ggtttgtctt
    13261 aggatccact gataaaattt ggaatttagc tggagatgtt tttcgtcctt cgatctttat
    13321 tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc ccttttacct ttgcaacgta
    13381 aactttatcc acttcatgtt ttggatgtgt caggcgctga gcaaaatctc catcatttgt
    13441 cagtagcaag atccccgaag tatcgtaatc cagtcgacct acaggataga tacgttcttt
    13501 taccatcggc aaaagatccg taacaacttt tcttcctttg tcatctgcaa cagcagaaat
    13561 aactccttta ggtttgtaca ataaatagta accgtattct tcttgataaa ttgggacttc
    13621 atccacttcc acagtgtcat gtttcccaac ttttatccct aattccctta caacttgtcc
    13681 gtttactttt acacgtcctg caaggatgaa ttcttctgct tttcgacgag aagtgatgcc
    13741 agcgtgggca attactttct gcaatctctc cattacaatt attctccttt attcattcca
    13801 tcaaagaata gatctggtga cacgtcttct tcaagtgaaa tttccatttc ttgtgtatca
    13861 ggcagttctt ctaatgattt caaaccaaaa taatctaaga agtagccagt tgttccatat
    13921 aatatcggcc gacctggtcc ttctactcta cctttttctt caatcagttt atgggcagct
    13981 agacgctgta ccgctcctga aacctgaact cctcgaatct gctcgatttc agctcttgtg
    14041 attggctgtt tataagcgat gattgctaag gtttcgacgg cggcttgaga taatgtgttt
    14101 gatgtccctt gcgcatatcg cttaagtaag ggagccagct cttttttagt cgttaataca
    14161 taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg agttttgagt gtaagattct
    14221 tttaattctt tcactaaact tgtgacttct gcttcgctga gatttaacag tccgcttaat
    14281 tcttcattac tgatcccttc ctcgccaaca ataaaaagaa gggcttcaag ttcacttaat
    14341 ttcgtcatgt tcggtatctc ctactgctgg ataaagcata attgattcat agttttcttt
    14401 ttgttgcgca atgatctgcc cttttttcat caattctagc aaagccataa aagtagtgac
    14461 aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa gtacagccat gcgcatttct
    14521 ttttatacgc tcttcgataa ataccattcg ttcttcgatc gttgtgtcat cgggcgtgat
    14581 cgttttttcc actttttgag aaaatttctt acgttctaag attttctgca tggcacggat
    14641 caaatcgatt tttttgactt gtcctcgctt caacggcttt tcagcatccg ctaacccatc
    14701 gatgttcata ggttcttttg tgtaataaag acttcgttcc tctgcttttt ctgtcagtct
    14761 ttcagctgca tatttatatt tacggtattc taaaagctga gtgaccaatt cttctcgagg
    14821 atcttcttcc cataattctt catcgtctgt gacgaattct tggacaggaa gcaatgtctt
    14881 gcttttgatt gccatcaatg tagcagccat cactaagtat tctcctgcaa cagctagctc
    14941 gagtgttttc attgtatgga tataattcat atattgctcc gtgatcgtag cgatcggtat
    15001 atcatatata tcgatttcca tttgctgaat cagatgaagc agcagatcga gaggaccctc
    15061 gaatacatct aatttaatat tgatctccgt caactcattc actctttctt ctttttcttt
    15121 gctagccatt ttccaatgta aggctctgtg atttcagttg cacggatgga gacatccggg
    15181 tacttttcct gtaattcatc taacatttga aagcccatat tctcaccgcg ataagacgga
    15241 ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg cccagtaaat accaataatc
    15301 ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa gttcataatc gtcttcagat
    15361 tcgtacgtat caatctcctt caacaaagaa gttttacctg tcagtttctt atggaaactc
    15421 aatagtccca tagcaatttt acggttttct ttccggtaat gtgttaacat cttctcacct
    15481 tcctatgact aagcccttgg aaaatgctgc ttataaacat ccgtcattct tttcttcgtg
    15541 atatgtgtat aaatctgagt cgtagagata tccgcgtgac ctaacaattc ctgtactgtc
    15601 cgcaaatcag ctccattttc tagcagatgt gtggcaaagc tatgccgcag cgtatgtggt
    15661 gttacttctt tgtaaatccc agcctctcgt actaactgct tcagattttt ccagattcct
    15721 tgccgagaaa gacctttccc atgattattg acaaatacat gcatttcaga tgcgtccttt
    15781 acaagtaatg gacgtgcttc gtctaagtag cgttccagcc attggatcgc ataatcacct
    15841 aaaggaatga tccgctcttt atctcctttt ccaactgtct gtaccagtcc aagagaaaga
    15901 tgaagatctc ccaatttcaa tccaatcaat tcactgacgc gcatcccagt agcatacatg
    15961 acttccaaga ttgcacgatc tcttatccct aacgtttttg tcgtatcagg tgcttcaatc
    16021 agcttttcta cttctgtcag agtcaatgta cttggtaatt tctgtgcttt cttcggcgta
    16081 tcgatatgtt gcatcggatc atgatcagtg atacgctctt gccgtaaaaa ctgatggaat
    16141 cttctgagac ttgagatcat ccgagtaaca gttgctgtcg cttttttttc ttcatgcaac
    16201 ttctccagaa aacggatgac taaaaagcga tcgacatcct gccatgaagc aattttctgc
    16261 tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat agctttgtcg tgtattggcg
    16321 gacaatcccc gttcaatatt caagtagtgg agataatcgt tgatttgctc tttcataata
    16381 atccccctca tgcctctcta tcatatcaga taccgcttca ttctgatagt ctttttccat
    16441 aatctgagca aagaaaaagc cgtctagaca ctcagacgga ctggtttctt ttttcttttt
    16501 catagcattc ctgacagatt ccatggaacg ttagccgatg atcttttact aaaaaatgat
    16561 aacgttcttc tactacttgc tccacttcaa gtaaaagatc ctcttctact tcctcgatcg
    16621 tgccgcagtt caagcatagt aaatgatgat ggaaatgttt ggcgccttct tttctcaaat
    16681 cataacgggc taatccatca ttaaaactta ctttatcgac aattttcaat tctgtcaaga
    16741 tttcaagggt tcgataaaca gtagcaagtc caatttccgg actctttttt ttcactaaaa
    16801 agaagatttc ttctgcagat aaatgctctt tctcattttc caaaagcaca agcagagtag
    16861 cttctctttg cggtgtcagc ttaaaccccg cttcatgtag ctgctgtttt gtttttttca
    16921 tcgccgaaat agcattcatg accatttact caaccccaac aatttataat aattacaaat
    16981 aaaaaaagat ataacactat gttaattaaa attattataa gctagttaag aaaaacatgc
    17041 aagggattct caattagatt ttttgactag ttttgtcaca ccgttttctt gtgtaatcaa
    17101 tccttgtttc aaaagattac ctaatgcgcg cttaaatgct cctttgctga taccaaaagc
    17161 tttcatgatt tcttctggat ccgatttatc tgtaaagttg atttgctgat ctgcagaacg
    17221 ttctaaaatg gttaaaatca tttgtgcatc atctgatata gcttcatatg ctcttggctt
    17281 caatgaaaga ttcagtacac cgtctggtcg gacaccaatc actcgacctt ttatctgctc
    17341 gcctagacga ggttcattat atcgttcaga aggatggata aacccaagat agaaatcttc
    17401 cgttaaaacg tacgtacctg ttaatttcaa gcggtatgct gttcctatca catctttgtt
    17461 tttcatttct tctgttccat atttgcttaa agatttgaat attttttcat ctgctaaact
    17521 gccccaaata cggtcttttg catcaactct aagggaaatc atcaagcgat cccctttttt
    17581 aggccatagc tctgtcattg tcggcaattc atcaagagat acgacaaagt ctttatctgg
    17641 aagaccgata tcgacaaacg cacctaaatc ttttcgtgta gcagtaacga ttccaaaagc
    17701 gtaatgaccg atacggcttt taggaatttg tgtagtgatc cgattttcat gtttttgatt
    17761 ttggtaaccg aatccttcaa ctgcttcacc gattttgtgt tcaccttctg atttgcttaa
    17821 gccaaaggtt tgtccgtttt tttggacaaa gtagtttttt tcattttcat caataataat
    17881 acctgtaaat atagttgcta ataaatcgtt catttttttc tccctctata gtgaaaggtt
    17941 ttcttaatac atttctttgc tatcataaca taatgttatc tacttgaata gctgagcctt
    18001 ggtttggaaa ggtgaaaaaa ggagtatgac aacagttgtg tcatactccc ctttttcgaa
    18061 taaacggtgt taaacggcag gctcacagcc tctttcattt gcctaaaatt aattgctccc
    18121 atttcctccg aaccatccag ttttacagtt gatggttacg atgtaaaatg gcaaagcact
    18181 ttctccaatg cctttcatca atgcagattt tgttgaagtg gcatatagat tataatagcc
    18241 ttccccattt tcgattctgg ctttttctaa tctgtaacct ccttcaacga tatatccacg
    18301 ttggatacat gtagcaatca ctttgctttg gacacctggt gcccacgtcc atgctggatt
    18361 agatgcatcg gcaatatcac ttgctctggc agatttgatt ggctgattcg ctgagtttga
    18421 agaggaaggg gtcgaggatg ccgcttcttg ctgtgctgct tgtgcgaccg cagcttgttg
    18481 agcggcttga gctgcagctg ctgcttgagc ggcttgtttt gcttgttctt cagctgcttt
    18541 tttcgcttct tctgcttttt tcttttcctc ttcttctttt ttctctttat cagtgagcat
    18601 cttatctact tgtttcaatt gttcattcaa ctgagcagat agatctgtat ttttcacttt
    18661 ttctactgct tcttttgccg tcttgtattg gtcgcgtgtt gctgaatcca ctacttttcc
    18721 gtctttaaag actatagctg ttttttcttt agctgtttgc aattcagcat attgattttt
    18781 agcttctgtt tgtgcttgtt gaatcaattt tccaaaatct gtgttatccg tttcgattgc
    18841 ttggatctct ttatctgctt tcaacttgat gtcatctgct agatgatcat cgacgatggc
    18901 aggtgctatg aaccactcat tcactttttg gatatctcgg atcttagatt ggatatcttg
    18961 gtaagttgtt tctaactctg aatatccttt ttcattctta agactggata aatcattttt
    19021 tagttttgat aaatcttgat gaatcatatc tgcacgaata aattgatggt gatcatctgt
    19081 ataaaaggca gcaagtttct catgaagttc ttccatttgt tgctgtcttt ttgcttctgc
    19141 tgctacttcg gcttctactt tttgatgatg gtcataataa gcccagcttc ctccaccgag
    19201 tagaagtact gctgctaaaa cgagataagg tacttttttt gattttttct tttgcgcttt
    19261 atctggatct gttttttcta agtttgattc ttgatgtgtt ttttcagtat ctggcacgat
    19321 tgttattgat ttcgcttctg cttcaaacag cttaggctgg ttctcttctt tttctatttt
    19381 tgcttcaaga tcagtaccct ctttgcttac agattgattt tcagcatcgg attcactatt
    19441 tatttcatcg ggctctgttg actttttgtc tgtgttttcg agtatttctt ttttctctgc
    19501 ttccggcagt ttattcgttt tttccttttc taattgtttt tcagtaaata cttcagtttt
    19561 ttctgtgata tccgttttta cgtcttttat ttttactgtt tctttacctg tggcgttttc
    19621 tctatcagag tctttttcag tttcttgttc cttttctggc tctactttgt tttcctgtgc
    19681 aattttttca ctgatcattt tgagcagctc tttcgcgtgc tcttcttttg tatcttcacg
    19741 atgtgctttg atataagcgg atagtatcga attttcttcc tcattatcga cgatttcttt
    19801 cagttcagct actcttcttt tttgttttga ttcaagcgtt tcttcttgat tctttcctga
    19861 ttcttccgaa gtctcatgaa cagttgcttt cttctgttca tttttctcta tattagttac
    19921 ttgatcgtga gcaggtggtt cttcgtcttc tggttctgct ttttcgaata attccattac
    19981 agattctaga ggtaaatctt tcaattccga ccattcgatt ggatcgttta attctgtatc
    20041 aggaaagact gcatcttcag ttgatttatc ctgcatagct gcatctgtat ttgtttcttc
    20101 aattgattct tttatttgct gatcttctgc agttatttga tgaccgcagt ctggacagat
    20161 agtattctgc tccatttttt tcgatccgca atttggacat tttttcatca cttagcgatc
    20221 ggctccttta ctaggtttac gctaccattt aatctatcat attctatacg tcacgccaat
    20281 agcttatttt taggatttac ttaaaaataa tgaatgaatt tccttcctat agaaaaaaaa
    20341 gcttgagact gcgtagtctc aagctttttt tcttttaaaa taaaccaaac agttttactg
    20401 atttctcttc tggtcaatag tccacgctta ttctaagaaa tcttttaatt gttttgaacg
    20461 agaaggatgg cgcaattttc ttaatgcttt agcctcgatc tgacgaatac gttcccgagt
    20521 aacaccaaat actttcccta cttcttccaa tgttctcgtc cggccatcat ctaaaccaaa
    20581 acgtaagcga aggacattct cttcccgatc agtcaatgta tctagtacat cttccaattg
    20641 ttcttttagt aactcataag ctgcgtgttc tgctggactt gttgcttcct ggtcttctat
    20701 gaaatcacct aagtgagagt catcttcttc accgattggc gtttcaaggg aaacaggttc
    20761 ttgcgcgatt ttcaagattt ctcggacttt ttctgttgga agatccattt ccgcaccgat
    20821 ttcttctggc gtaggttctc ttccaaggtc ttgcagcaat tgacgttgga tacgtatcaa
    20881 tttattaatt gtttcaacca tatgaactgg aatacggatc gtacgtgctt ggtcagcgat
    20941 tgctcgagtg atggcttgac gaatccacca tgtagcatag gttgaaaact taaatccttt
    21001 gcggtagtca aatttttcaa ctgccttcat cagtcccatg tttccttctt ggatcaagtc
    21061 aaggaactgc atgcctcgtc ctacataacg tttagcgata cttactacta aacgaaggtt
    21121 tgcttctgcc agacgctgtt tcgcttcttg gtcgccttct tcgattttca atgctaacgc
    21181 tacttcttct tccgcagtta aaagggagac acggccaatt tctttcagat acatacgaac
    21241 cgggtcattg atttttacac cggttggcgc agaaagatct tctgtttttg cttgttcagc
    21301 ttttttctca gcactcttca agctgtgaat tgatggatca ccattttcat ccaccacact
    21361 gatacctgcg tcttcaatct tttggatcaa tttgtccatt tcatctgcat tcaaagtgaa
    21421 tggtgtagca agctgattgg ataaatcatc atatacaact tgtcgtttgg gtttgttttc
    21481 tttgatgaaa gctgctactg ctgcttcata cgttttttta tctgttccat ttgccataaa
    21541 agaaggcccc ccttgagtag ctatttggct tgttttaact gtttcgttaa attgatgatt
    21601 tcaatcgtca attcaagctc aagctgtttg tttccagttc gtctggcttc ttgctgcatt
    21661 tgttttttta atgttatttc atcagctaag ccagatttgc tgatcacatc tagcaagtct
    21721 tggatttctc gtggcgaact ttcttctgcc accggtaaat attcgatttc caccaccaac
    21781 cgccttaaat tttcatcttg caaaaagtct aaaaactctg caaggttgaa ttcataatgt
    21841 tgtgagacat atgcatcgaa taaaaaataa acttctgcat atgcatcatg aataaactga
    21901 aattctcgtt cttttaaaat attacggaca gaaacttctt taaatgcacg atataataga
    21961 atctgctctg ccttttctac ttgcgtcaaa ggtctctttt tccgtgcagt ttcttgtctc
    22021 gtaacctgag tgtgttgact ttcctgaggt tcttgacgat tttgctgatt tgtccttcga
    22081 agatcgcgca gttgtttttg tatcgtctct ctgccgatct gaaactctgt tgacagttga
    22141 gatagataca tatcttgttc gattggtgaa acaactcggc taagttcaac tagtaattct
    22201 tcgaggtatt cgatcttgtc tttttcatta tctaaatttt tcccttgttt taaataacgt
    22261 gatttaaaag taaagactgt ttcgcgccca tgagccacta attcttggaa agatgcttct
    22321 ccatacttac ggagatattc atcaggatca agcttttctg gaacgactac gacagaaagt
    22381 gctaatcgac tgttttgccg aagcaattca atccctcgat tggttgcttc aaaacctgcg
    22441 ttgtctccgt catagcaaaa gaccaattct ttcgtcagct tttccatttg ttggatctgt
    22501 tgagctgtta gacttgtccc catagaagct aatccagttt tgataccagc ctgccatgca
    22561 gcaatcacat ccataaaccc ttcgaacaac aaaatttctc cgtttttccg gatatcgctt
    22621 cttgctttat caagattaaa taaaacttgt cttttattga acagttctgt ttctggacta
    22681 ttcaagtatt tgggctgatc tttttcctgc cctttttcag gtgcaagcca gcgtcctgaa
    22741 aaaccgatcg tctggccttg agaattacga agtggaaaca tcactctttg ataaaaacga
    22801 tcagacatac ggccatcatc atgcgtgaca aacaatccag agcgttctaa caattcattt
    22861 gaaattgatt cattcttgaa tacttgctga agaaaagaac gctcattagg tgcaaaacct
    22921 atgttgaatt cttcaattaa ttctaaagac aacccacgac tttgcaaata ttctaaagct
    22981 ggctgtccag cttttgtatg caatagcatg tgatgataga tttccgctgc tttttcatga
    23041 gcagcaatca gtttccccgt agcagattca ggattttgga cagctatagt acgctgacgc
    23101 cattgatctt ctaacggtat ctgttcgaag tctgccactt ttaccactgc ttctggaaaa
    23161 cttagacctt ctagttcttg aagaaaagta aacacgtttc cgcctttacc acaaccaaag
    23221 caatgaaaaa tctgcttgtc ttctgcaaca gaaaaagacg gtgtacgttc ttcatgaaaa
    23281 ggacacagcc ccaagtaatt tttaccagac tttttcaact ggacatattg tccaatcacc
    23341 tcaacgatat tcgtttgact gcgtactgtt tcgataacct cttgaggaat ccgttgagcc
    23401 attttttcac cccctgctct attgatatct tttgggttta acagaagata tttcaggaga
    23461 ataaccggta aaaaatcgca ctaactgaaa tcagtgcgat tttaaaggac acaattatac
    23521 atgtttgatt gtatcatatt tcattgcttt ttcaagtata acacatttct atctcattaa
    23581 gcatcaattt gctactcttt tcagtaactg attttgaata tcagccttcc agtacaatac
    23641 tcgaagaaca atatttacga gcatgaatac aaagaagtat cctacgctct ctcccaaccc
    23701 taagtttact tccaaaattc catggaacag cattgcagct acatatatcc ctacgatcgt
    23761 cgttactgct aggaagatgc gcaatgggtt gaagggataa caagctttta caactgccat
    23821 gcaactgatc ccaatcagca agtaatacat caaagtagta gtttcagcat ttccccatgc
    23881 gaatgttttt ccaagtagat acaccgcaat gatattcact actacaagca aggcatttgg
    23941 taaagcatta ataagtgctg taggcaaata acggtaagtg atttttcttt tatcttgttc
    24001 aaaagataaa aagaaggaag gatacccttc aatcgctaaa tcaatcagcg tgatttggat
    24061 agggataaac ggaaatccaa ttgctgtaac ggcacatata atcgataaaa taaatgaata
    24121 aatcgttttg ataaaaaaga tgcctgagac tttcgtcaca ttgttgacca ctcggcgtcc
    24181 ttcaaacagg acttctggca atgtagtaaa atcagagtcc aacaatacca gattcgcaat
    24241 ctgacgtgtt gctccatcac cttctgccat agcaatgctg caatctgctt ctcttaacgc
    24301 caaaacatca ttcacaccgt cgcctgtcat tgcaaccgtt ctgccgcttt ctttcaattc
    24361 atttaccagc aatttctttt gctgcggact cactcgtcca aaaaccgtat agcgatgagc
    24421 agcttctcgc acttccattt cttcagtaat ttgagaaaga tcgacatacg catcataatt
    24481 cggtaatcct gcacgtctag cgatattgga gacagtaaaa ggattgtctc cagaaatcac
    24541 tttcagatcc acaccttcct catgaagata agctagtgtt tcttgagcgt tttttcggat
    24601 tggatcatcg atttcaaaca gagcaaccgg ctcaacaaat gctggttttt cttcgttcaa
    24661 ttgctgattt ggagcgatcc ccaacataag tacgcgatag ccattcgctt gggctgtttt
    24721 tagactgtcc ggaagccgat cttcttccac caaacgttca ggcgctccta cgtacacgac
    24781 tcccagttct ggaaattcca tcgcgcccca tttgcgatca gaagaaaatg cgactttgtt
    24841 tgtcacatca taccgattag aaagctcaaa ataatctctc atagcccgca tagtgatgtt
    24901 gttatcactg ctttctgcca gatacgtccc cataatttct gggatggctt tctcgtacag
    24961 cggatgtagt gcttctaccc gctggatact cattttccct tcagtaatgg tccctgtttt
    25021 atccaagcat aatgtatcga catgggccag cgtttcaatt gaatacatgt cttgaacaag
    25081 gattcgtttt tttgccagtt tggttacccc tgtcgttaat gcaatactta ttagcaatac
    25141 caaacccttt ggaagcatgc ctaacaaagc tgctgcagat gcaataacag aaattttgat
    25201 agaagcatcg cggatgaaca gtgcttccaa aaatagtatg atccccaaag gaataatcac
    25261 gaaactagtg aatttggata ctttgcgaat cgaatctaca agttcagaac gaatagcttt
    25321 gtgagtcttt gcctcgctag tcaagcggac agcataatta tctgcaccta ctcggataac
    25381 ttcagccatg acagaaccgc ttgtcaaaaa gcttccagaa agcaattcag ctccagtttc
    25441 tttgacaatc aaatcagatt cgcctgtcaa aagagcttca tttgcttctg cccgtccaga
    25501 tataacctgt aaatcggctg gtatctgttc tcctgctccg atttttacca aatctcctaa
    25561 taccagctct ttagtagaaa tcgattgttc tttaccgtcc cggatcacta gtacttctga
    25621 ttgggaaaca atcgataatt tccgtacaag atttcttgca tgtatttctt gataaattcc
    25681 aataacgata ttgacgacaa taattgctaa atacgccatg ttggaatacg cacctacaaa
    25741 taacagacaa atcccaatac caaagttcag aaaattgaat aacgtcaata tattatcgct
    25801 gaatatttgt tttgtacttt ttgatgcatc ttcctggtag tcattttgct ggccagaagc
    25861 cattttctga actacctctt gctgtgtaag tccattcatt ttctttttct ccattttccg
    25921 ccaactttca aaaataatca cgtgtttaag tctagcgtgt tccaagatta aaaacaactt
    25981 gtttttataa aatttataga aaaacacacg aatgatttta caaaaaagat gccaaccttt
    26041 ttgtttgaat cacccgtgtg ctgtttttat tatcaatcta tctttactta tattgttcct
    26101 ccaaacggct catccaccaa cctagtgctg taccggctat aaaaagaaca ggacaagcta
    26161 gtaccccagc aactcccaat cccgcgtaat cggtaggaat gatcataatc gttgaagcaa
    26221 aaactacacc taaaatgaaa tggaatagct tgctatatgc ctttgagaaa atgtaatcca
    26281 tgatttttga caatccaaga acacaaagaa tcccaccaat tccgatcggc acaatcactt
    26341 caagtcgtac ttctttaaat ccgtcagcca tcgctttata caatcccata tacaccaaaa
    26401 aattagaagg gctcaatcct gggacgatca tacccaaacc aatcaatccg ccggcaatca
    26461 gccatgttcc aaaattctga tcgacttgcc cgtcaaataa ttgagcgccg tataataaaa
    26521 acaaataagc cagaacaaaa gtaacaacca tgatgatcac atcagaagta tctcttcctt
    26581 ttttccctgc ttctttccat aacgcaggaa cagttccagc gatacatccg acaaaaaacc
    26641 ataatatcgt tgtttcatac gcacctaata aaaaactgac taaaaaagaa agtagaaaga
    26701 tgcctgtcac tccgcctaga ccaacaggca gaaaaaacaa aacattttct ttgaaatttt
    26761 ttgtaaggtg ggctaaaaaa gaaatcagcc gctcatacat gccaaagaca gctgctaatg
    26821 caccaccgct gactcctggc aagataaatc ctgaaccgat aaacattccc ttcacaaacc
    26881 gtaacatcca gtctttggtt gtgtacccca cttcttgatt ttccattttt tgcctttttt
    26941 ccactatttg cgtactcctt ctttcattta attttattca tgattgaaca tttcctgcta
    27001 ttttacttaa agactttttc ataggtctct attatttttt cattcatttt gacaatatca
    27061 ggataaaaca tgttacctcc attatgatag agatagccgc cattgtaaag caaggactgg
    27121 atccgccaat atcggtattg tttcccttcc tcatttccta aaagtgggct cagcacatct
    27181 ttagaatacg cttctgctaa ttctactgtg ttttctccgc catgattttt tacataggca
    27241 atataatctt tcccaaaatt atatgcttgg acagctgtcc ataaatcgca tccttgtttc
    27301 tttgcttcta ctatcatttc cgccaaatat ttgacacctt gatcaatact gtcagctgga
    27361 ttatcaatga tctcagcttc tccatgcaga ctttccgagc tctgcatgag atctgtccct
    27421 tttccttttg attcggtcaa tattatcgcc ttgatcaaat tctcgtagtt ttccatatga
    27481 taattttttg cagctgttgt tatttctatt tgatagcttt ccactcgttt taccgaacga
    27541 taagttcttc ctcataaaaa caacaccgca ccaataaccg tcagaatgat catgtaaaaa
    27601 agaatacgga taatagataa tcgttttcgc ttcatttcat cacctagtta ttttgacatc
    27661 tttttttatc ttactatttc tttatcttct tggctatttc ttccttgatt tctgtgtacg
    27721 ccttaagaaa aaatgaattt tcctgtattt tttgacatct tacgaaaatg atcgttgttt
    27781 ttcagtaaaa acaattgccc aatgactgat ataatcgtta tactttacac agtaggtgat
    27841 tatgtgaaaa aagaaaagaa atatgcaaaa atgccagatg ggagcgaaat ttactatgaa
    27901 aagagcggtc aaggcttccc tctcttttta ctgcatggca atgacggcag cggccgcttt
    27961 ttttcagaac aggtccctgt actcgaacgc tactataccg tgtatctagt agatagccga
    28021 ggacatggtc gttcgacaaa cgaagcatct atgttgaatt ttcagttaat ggccgaggat
    28081 ctgaatacga tcatgttgct ggaaaaaatc gatcaggcag attttcttgg atttagtgat
    28141 ggagcaaatc tagcactggt atttgccagc agcttcccta aaaaagttca tcgattgatt
    28201 ttaaattctg gaaacacgct tgttaaaggc gttcgttttt ctgccagagt catcagtaat
    28261 attcattatg catgggtatg gttactctct ttatttcgcc caagtcttcg caaaaatctt
    28321 ttagttatca aacttttgct gcatgacatc gggcttacag aaaatgattt aaaaaaaatc
    28381 aactccccca ctttgatcat cgtgggaaaa aaagatgtaa tcaagctaaa acattctttg
    28441 tatatagcta aaactatacc aaaagcttcc tttgtgcttg tgaaagaaca aggacatgaa
    28501 ttagctagaa aagatcctga acgcttcaat cgagaagttc ttcaatttct ttctgaaaca
    28561 taactattag gaatagaagg tgatttattt gttaaaaaat accatacaat ggctgaaaaa
    28621 ccacctagga ctgttcaaaa caatcttttt gatttctgtc attgtcatta ttgtcggtga
    28681 attgatgtcc atcggtaaaa ctctatctat ccagcaatta ggagaaacgt ttgccactat
    28741 acctgtatgg aaaagcggac taatgctatt gattggtctt atctctgtgt tacctatggt
    28801 tggttatgat attattttga atcggatgct tgaacaaaag cagaatcctc gttacctgtt
    28861 tgagacaagc tggctgatca atacgatcaa caatattgcc ggtttcggcg ggtttgttag
    28921 tgtggggctt cgctctgaac tatatggaca aaagaaagag agcagaaacg tgatccaagc
    28981 attatcgaaa gttttcttgt ttttgatggc tggactttct atttacagtc tgctttcttt
    29041 tttacttgta gtcttcacgc cagttgctcc ttttttgaag caatattgga tatggttgat
    29101 cggcggcggt ctatattttc ctgtcgtctt tttatttact actttcaaaa agaaaggatt
    29161 ccttggtggt acctcactta aaactcaagg acagctattg ctagtttcat tgttggaatg
    29221 gaccggtgtc ctgtttagtt tcctttctgt tggttattta atggaaatcc ggattgatct
    29281 gtggcaaaca attccattat tcattgctgc ttcagtgata ggaatcgtgt ctatgattcc
    29341 aggagaaatt gggagtttcg atgtaatgat gattatcggg ttgtccgcta ttggtgtgcc
    29401 aagagaaata gtcgttgtgt ggatcttgct ctatcgcctg ttctactaca tcattccgtt
    29461 tttgattgga atcgtctttt tcttcaaaaa tatcggttcc acttttgatc agcgttattc
    29521 tggtatccct aaacaattag caacagaaat tgcgcataaa atcgtggttg tcttattata
    29581 cttttctgga atcatgctag tactttctgc cactatcccg caagctttta cagagtttcg
    29641 ctggcttcat agtttgaacc cattgaaatt tcattttatt atccaatttc ctagtatact
    29701 attgggattt cttttaatca ttatgggcag agggatcgca gcacgtgtga aacgagcata
    29761 tttgcctaca atcttcttga ttgttctagc tttactttat gttttattaa gtgattttag
    29821 tttcacaccg gtggttttct tgtctattct gcttctgatc attcttgctt ctaaaaacga
    29881 attatttcgg gaacaattga tctattcatg ggagtggcgt acgatcgatg gaatcattat
    29941 tggtgcactg tccttgcttt acatcgttat tggggtatac aatctgccgg atttccctca
    30001 tcggcgtcac cattttattt cctttttctt atttccttct gaaaagattt ggttttcggg
    30061 attgttggcc atcatcgctg ttagtttcat gatcgttcta tttgttcatt tccttcaagg
    30121 agaaaagaaa cagattggcg aagcctttaa tgaagaaaag gccctgaaga tccttacaac
    30181 ttatggagga aactccgata gtcaattgat cttcttaaaa gataagcgga tgtttgccta
    30241 tgaaaaagac ggtgaaccaa ctgttcttct ccaattcgct tgcttcaata ataaatgtat
    30301 tgtaatgggt gatccttctg gtaagaaaga agattttcca gaagcaatcg aagcttttat
    30361 cgaagaaaca gatcgactat gctatttacc agttttctac gaaacaagcg aagagattgt
    30421 tatgattttg catgaattcg gctacgattt cattaagatg ggggaagaag cttatgtaga
    30481 cctgaatagt tttacaactt cagggaaaaa aatgaagggc actcgtgctg tcttgaatcg
    30541 gatcgaacga gaaggattta catttgatgt cttacaacca ccattttcta ctgagcaaat
    30601 gagtactttc aaaaatatct ctgataattg gttgggttca cgaaaggaaa aagggttttc
    30661 tttaggcttt ttctctgaag attatctaca aagagcgcct attgcagttg tcaaaaatac
    30721 ccaagaagaa attgtcgctt ttgcaacgat catgcctact tacacgaaca ataaagtagg
    30781 aacgattgat ctgatgcgat atgattctaa aaaagcacct tctggcagta tggatttctt
    30841 gttcttgcat ttgtttgcct atatgaaaga agaacatatc caatggttca atttagggat
    30901 ggcccctctt tcaaatgtcg gaacttccag aaagagtttt ttacaggaaa ggattgcata
    30961 cttggtttac gagtttggtt ctcattttta ttctttccat ggattaagag aatataaaag
    31021 caagtacgct acaaactggg tctcacgtta tactctatat tctagagata gttggattgc
    31081 atatgttatg attgctttgc tgattattga taatgcgcca gttgagcaga cacaaggtag
    31141 aataagtggt ttgaaaaaat ggatacgaag aaagtattaa aaactgcata ccgatagatg
    31201 aacactctca tgatgaataa aagagactga agtaaaacga aagttcatgc ttctacaatc
    31261 tcttcttata caattgaaga acaaagagcg gtccaaacag aaatcctgtt tggaccgctc
    31321 ttttatttta aacatttttc attaccaaaa tattcgagtc gcgtttctta tgcttcgatc
    31381 tctgtaacga tacctgaacc tactgtccgt ccgccttcac gaattgagaa tgtcgttccg
    31441 ttttcaacag cgattggatg gatcaagtca acatcaatcg tcacgttgtc accaggcatg
    31501 accatttctg tatcttctgg caatgtgatt gttcctgtaa catcagttgt gcggaagtag
    31561 aactgtggac gatagttgtt gaagaatgga gtatgacgtc caccttcttc ttttgtcaac
    31621 acgtacactt ctgctttgaa ttttgtatgt ggagtaatgg aacctggttt agcaagtact
    31681 tgtccacgtt cgatatcgtc acgttggatc ccccgtaaca acacgcctac gttatcccca
    31741 gcttccccgt aatctaacgt tttacggaac atttctacac ctgttacaac tgctttttgt
    31801 gtttcaggtt tgatccctac gatctctacc tcatcaccga cacgaacagc accacgatca
    31861 atacgacctg aggcaacagt tcctcgacct gtaattgaga agacgtcttc cactggcaat
    31921 agtaatggtt tatctgtatc acgttctggt gttgggatat attcatctac tgtatccatc
    31981 aattccataa tagcagcttc agcatctgga tcgccttgca atgcttttaa tgcggaacct
    32041 ttgatcacag gagtgtcatc gcctgggaaa ccatattcgc tcaataactc gcgaacttcc
    32101 atttctacca aatcgatcaa ttcttcatca tcgactaaat caactttgtt caagaaaaca
    32161 atcaggtatt ttacaccaac ttggcgtgac aataaaatgt gttcacgtgt ttgaggcatc
    32221 ggaccgtcag ttgcagaaac aactaagatt gcaccatcca tttgtgctgc ccctgtaatc
    32281 atgtttttca catagtccgc gtgtcctggt gcatcaatat gcgcgtagtg gcgtttttct
    32341 gtttcatatt ctacgtgtgc ggtattgatc gtaatcccac gttcacgttc ttctggagct
    32401 gcatcaatac tagcatagtc ttgaggattt gctaggcctt ttttgcctaa tactgtagtg
    32461 atcgcagcag ttaaagtagt tttcccatgg tcaacgtgtc caatcgtacc aatgttaacg
    32521 tgtggtttac ttctgtcata atgttctttt gccataattt acaaacctcc taaataatat
    32581 agatcactga aaaatttcag ctctcacctt tttattatag tctttttttg actaaatcaa
    32641 aaagaatacg tccgacttac tttttgtaag attcgaacta agcttattat acgtcaatat
    32701 tggtcaaagt caactaagag cactcgtctg tcttatacgc gaaaaaagta ccttttataa
    32761 ataaaaaaat gtgttttttg agaaaaactt caagatagtc cttaactttc ttttgaagca
    32821 ataagaacaa aatgaggaaa agtttaaaat tcagctgttg ccaacaactt tcaatagacg
    32881 aaatcttcta tttcgttaat aataaggaaa gaggtgaagt acatgaaaaa attaattggc
    32941 gtacttgtcg ttttaggcat atttctcggc ggaagttttg cagcttatca ttatttttac
    33001 ggcggcgaag cttattatac aaaaatcacg acgaatggcg aaatgtcagc tacccaagca
    33061 gataacggag agaaattcac gacttatacc tacaagcaag cggcttataa taaagatggt
    33121 gaaaaaaaac aggtgacgct tcgcgaagaa agagaacatc cattaaagat gaatgcgtat
    33181 cttaaactca aagtcaatcc tcgtaaaggt gtattaagct gggaagaaat aaaggcttct
    33241 gaagtgccta aaaatgcggc agaaaaaatc gataattagt aaatggtctt aaaattttat
    33301 tagataaaa
//
LOCUS       DAYYHX010000007        31896 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000007 DAYYHX010000000
VERSION     DAYYHX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.007"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..601)
                     /locus_tag="ACHA6Y_000234"
     CDS             complement(26..601)
                     /locus_tag="ACHA6Y_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM8986360.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="ACHA6Y_000235"
     CDS             complement(606..1580)
                     /locus_tag="ACHA6Y_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFM8986361.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="ACHA6Y_000236"
     CDS             1863..2831
                     /locus_tag="ACHA6Y_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8986362.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="ACHA6Y_000237"
     CDS             2928..3221
                     /locus_tag="ACHA6Y_000237"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8986363.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="ACHA6Y_000238"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="ACHA6Y_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM8986364.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="ACHA6Y_000239"
     CDS             3678..4433
                     /locus_tag="ACHA6Y_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8986365.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="ACHA6Y_000240"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="ACHA6Y_000240"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM8986366.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="ACHA6Y_000241"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="ACHA6Y_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM8986367.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="ACHA6Y_000242"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="ACHA6Y_000242"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM8986368.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="ACHA6Y_000243"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="ACHA6Y_000243"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM8986369.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="ACHA6Y_000244"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="ACHA6Y_000244"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM8986370.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="ACHA6Y_000245"
     CDS             9170..9712
                     /locus_tag="ACHA6Y_000245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM8986371.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /gene="atpA"
                     /locus_tag="ACHA6Y_000246"
     CDS             9735..11291
                     /gene="atpA"
                     /locus_tag="ACHA6Y_000246"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM8986372.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="ACHA6Y_000247"
     CDS             11305..12207
                     /locus_tag="ACHA6Y_000247"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM8986373.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="ACHA6Y_000248"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="ACHA6Y_000248"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM8986374.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="ACHA6Y_000249"
     CDS             13650..14072
                     /locus_tag="ACHA6Y_000249"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM8986375.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="ACHA6Y_000250"
     CDS             14541..14771
                     /locus_tag="ACHA6Y_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM8986376.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="ACHA6Y_000251"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="ACHA6Y_000251"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8986377.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="ACHA6Y_000252"
     CDS             16182..16358
                     /locus_tag="ACHA6Y_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8986378.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="ACHA6Y_000253"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="ACHA6Y_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM8986379.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="ACHA6Y_000254"
     CDS             16853..17320
                     /locus_tag="ACHA6Y_000254"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8986380.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="ACHA6Y_000255"
     CDS             17405..18289
                     /locus_tag="ACHA6Y_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8986381.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18403..18639
                     /locus_tag="ACHA6Y_000256"
     CDS             18403..18639
                     /locus_tag="ACHA6Y_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM8986382.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="ACHA6Y_000257"
     tRNA            18727..18807
                     /locus_tag="ACHA6Y_000257"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="ACHA6Y_000258"
     tRNA            18814..18885
                     /locus_tag="ACHA6Y_000258"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19011..19181
                     /locus_tag="ACHA6Y_000259"
     CDS             19011..19181
                     /locus_tag="ACHA6Y_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986383.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19401..20108
                     /locus_tag="ACHA6Y_000260"
     CDS             19401..20108
                     /locus_tag="ACHA6Y_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8986384.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="ACHA6Y_000261"
     CDS             complement(20167..20811)
                     /locus_tag="ACHA6Y_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM8986385.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="ACHA6Y_000262"
     CDS             20938..21225
                     /locus_tag="ACHA6Y_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986386.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="ACHA6Y_000263"
     CDS             21333..21653
                     /locus_tag="ACHA6Y_000263"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8986387.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21734..23047
                     /locus_tag="ACHA6Y_000264"
                     /pseudo
     CDS             21734..23047
                     /locus_tag="ACHA6Y_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            23044..23763
                     /locus_tag="ACHA6Y_000265"
     CDS             23044..23763
                     /locus_tag="ACHA6Y_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM8986388.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKDYPAYIYCPIPLSEKRWLERGFNQVSEMLKAAKI
                     PFQMLLKREQHLLAQAKNTRKERLEMPQPFELAVPLSKIQHKKILIIDDVYTTGQTLF
                     HAADCLQNAQPKIIRTISLAR"
     gene            23891..24442
                     /gene="raiA"
                     /locus_tag="ACHA6Y_000266"
     CDS             23891..24442
                     /gene="raiA"
                     /locus_tag="ACHA6Y_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM8986389.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            24664..27198
                     /gene="secA"
                     /locus_tag="ACHA6Y_000267"
     CDS             24664..27198
                     /gene="secA"
                     /locus_tag="ACHA6Y_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM8986390.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            27324..28425
                     /gene="prfB"
                     /locus_tag="ACHA6Y_000268"
     CDS             join(27324..27395,27397..28425)
                     /gene="prfB"
                     /locus_tag="ACHA6Y_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM8986391.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            28530..29216
                     /gene="ftsE"
                     /locus_tag="ACHA6Y_000269"
     CDS             28530..29216
                     /gene="ftsE"
                     /locus_tag="ACHA6Y_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM8986392.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            29209..30093
                     /gene="ftsX"
                     /locus_tag="ACHA6Y_000270"
     CDS             29209..30093
                     /gene="ftsX"
                     /locus_tag="ACHA6Y_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM8986393.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            30245..31693
                     /gene="cls"
                     /locus_tag="ACHA6Y_000271"
     CDS             30245..31693
                     /gene="cls"
                     /locus_tag="ACHA6Y_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8986394.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT        10607 a         5565 c         6908 g         8816 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa agattacccg gcatatattt attgtccaat acctttatct
    23461 gaaaaaaggt ggctagaaag aggattcaat caagtaagcg agatgttgaa agcagcaaaa
    23521 attccttttc agatgctgct caaaagagag caacatctgc ttgctcaagc aaaaaacacg
    23581 cgaaaggaaa gattggagat gcctcagcct tttgagcttg ccgtacctct ttctaagata
    23641 caacataaaa aaatattgat tattgacgat gtctatacaa caggacagac gctttttcat
    23701 gcagcagatt gcttgcagaa cgcacagccg aaaatcatcc gaaccatttc tttggcaaga
    23761 tgaatcagat gtggaaattt gatagcgatt taatttgcaa ataacctaga gttcgatata
    23821 atagaattaa gaagagggaa agagtggtcc ttctccttct tgattgtggt agacgaaagg
    23881 ggtaattttt atgtttagat ataatgtacg cggcgaaaat atcgaagtaa ctgaagcgat
    23941 tcgcgactac gttgagaaaa aagtaggcaa actagaacgc tatttcagtg acgcacccga
    24001 tgcaacggcg catgtgaact taaaagttta tacagaaaaa acagcaaaag tggaagtaac
    24061 gatcccatta ccttatcttg tattacgtgc cgaagaaact tctccagatt tatacgcaag
    24121 tatcgatttg gttgttgaca aattggaacg ccaaatccgc aaattcaaaa caaaaattaa
    24181 tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct gctgctttat tcaatgaaga
    24241 ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt acgaaacgcc tttcattgaa
    24301 accaatggat agtgaagaag cagtcttaca aatgaacatg ctgggacata acttctttat
    24361 ttttgaagat gcagaaacaa atggtacaag tatcgtttac cgccgtaaag atggaaaata
    24421 cggactgatt gaaacagact gatcagcctt acttaaataa caacgaaaac ttgcagcagc
    24481 tactgacagc tgccgcaagt ttttttgagt ttcctcctct tttagatgaa atatgaataa
    24541 attatgaagt tcttacttta atacgcttaa gatatttcaa ttatctataa ctaatgataa
    24601 aatgaaaagt aaagtctaca tagtctggat aatacacaga aggaaaagga gattacacaa
    24661 cgcatggcca atttgattcg ttcacttatc gaaaacgata agaaagaact aaaacgttta
    24721 gatagcattg caaaaaaagt tgaagcatac gctgaccaaa tggcagcatt aactgatgaa
    24781 caattacagg caaaaactgc tgaattcaag ggcagatacc aaaaaggaga aacattagat
    24841 caattacttc cggaagcttt tgcagtcgtc cgagaagcag caaaacgtgt attaggttta
    24901 tatccatacc acgtacaatt gatgggtgga atcgtgcttc acgatggaaa tatccctgaa
    24961 atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc cagtatatct aaatgctctg
    25021 actggcgaag gggtacacgt agttaccgtc aatgaatatt tagcaacccg tgactcgacg
    25081 gaaatgggcg aactttataa tttcttagga ttaacagtgg ggttgaatat caactcaaaa
    25141 tcttcggatg aaaaacgcga ggcttataat tgtgatatca cttatagtac gaataatgaa
    25201 ttaggatttg actacttgag agacaatatg gtcgtttacc gccatcaaat ggtacaacgt
    25261 ccgctgaatt atgccattgt cgatgaagtt gactcaatct tgattgatga agcgagaacg
    25321 ccgctgatca tttcagggca agcagaaaaa tccacagctt tatatacaag agcagacaac
    25381 ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg atgtacaatc aaaaacaatc
    25441 agtttaaccg aagcagggat cgaaaaagca gaagaaacat tcggtttgga taacttatac
    25501 gacattgaaa atactgcatt gacccatcac ttggatcaag cattgagagc caactatatc
    25561 atgcttttag atatcgatta tgttgtccaa gacggaaaag tactgattgt tgaccagttc
    25621 actggtcgga tcatggatgg acgccgttat tcagatggac ttcaccaagc aatcgaagct
    25681 aaagaaggcg tagagatcga agacgaaacg aaaaccatgg cgacgatcac tttccagaac
    25741 tatttccgta tgtacaagaa acttgcggga atgactggta cagctaaaac agaagaagaa
    25801 gaattcagag aaatttataa catccaagta taccagattc cgacaaatcg tccggttatc
    25861 cgtgatgatc gtccagattt gctttaccca acattacaaa gcaagttcca cgctgtcgtg
    25921 caagatatca aagatcgcta ccgcaaaggt caacctgtgt tagtcggaac tgttgcggtt
    25981 gaaacatctg agttattatc agatatgctg aatcaagaac gtattcctca tgaagtgctg
    26041 aatgcgaaaa atcactttaa agaagcagag atcatcatga atgctggtca aaaaggtgct
    26101 gtgacgattg ctaccaacat ggctggtcgg ggaacagata tcaagttagg acttggggta
    26161 agagaacttg gtggtctagc agttatcggg acagaacgac atgaatctcg tcgtatcgat
    26221 aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg gtatgtcaca gttctacttg
    26281 tcattggaag atgatttgat gaaacgattc ggttcagaac gaatcaaagc tttcttagat
    26341 cggatgaaga tcggtgagga agatgctgtt atccaaagca agatgctgac gaaacaagta
    26401 gaatctgcac aaaaacgtgt agaaggaaat aactatgaca cacgtaaaaa cgtcttacaa
    26461 tacgatgatg tgatgcgtga acaacgtgaa gtaatctata aacagcgaca agaagtaatc
    26521 atggaagaaa aaagcttatc caagccacta atgaacatgg tcaaacggac aatcagtcgt
    26581 gtagtagatg cgcatacaca attagagaaa gaaaattgga atttggaaag tatcatcgat
    26641 tttgccggca gtactcttgt ccatgaagat agcattactc ttgcagatat cgaaggaaaa
    26701 acagctcagg agatcaaaga gtatctaaac gaacgagcac aagaagtatt taataccaaa
    26761 gcggctcaac taaatggtcc agagcaactg ttggaatttg aaaaagtcgt gatcttgcgt
    26821 gttgtggact ctaaatggac agaccatatt gatgcaatgg atcaattacg tcagtcaatc
    26881 ggcttacgtg cttatggaca gaacaatcca ttagtcgaat atcaaacaga aggctataag
    26941 atgtttgaag atatggtagg cgccatcgag tatgaagtca ctcgaatctt tatgaaagct
    27001 gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag gaacagcttc tcactcagaa
    27061 gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa aacagccaat ccatgtgaat
    27121 aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga aaaaatataa gaactgtcat
    27181 ggaaaaaatg caaactaatt tctgacagca ggattggtga gacaaattct ttttttaaga
    27241 attcgtctca ccttctttct gttatttgct gattctgtta gactagatat aatgaaaaaa
    27301 gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat tcgaaatctc ctgacagaaa
    27361 tgcaggaaaa ggtcacaagt ttcaggaggt ctctttgact tagatcagct ggaagaagac
    27421 atcgcggaag cagaagcaaa aatggctgaa cctggttttt gggatgacag cgaagccgca
    27481 caaaaagtga tcagcgaaaa taacgctaat aaagaaacct atgaccaatt ccattctctt
    27541 gccgaagaac tagaagaaat cgaattgatg gtggagatgc tgcaagaaga acctgatgca
    27601 gcaatgcaag aagaagcaga aacacgtatc aatgcattag atgaaaaaat gaagacgtat
    27661 gaattatcta tgctgttaga cggaccttat gatcgaaaca atgcgatcgt cgaattgcat
    27721 ccaggggctg gcggaacgga gtcccaagac tggggcagca tgcttttacg tatgtataca
    27781 cgttgggcag agcagcatgg ctatcaagta gaaacactag attatcaagc aggtgatgaa
    27841 gcagggatca agagtgtgac cctgctgatt aaaggacata acgcttttgg ttatctaaaa
    27901 tcagaaaaag gtgttcatcg ccttgtacgg atttcgcctt ttgactcagc gaaacgacgt
    27961 catacttctt tttgttcggt agatgtgatg ccagagcttg atgaaacgat cgatatagaa
    28021 atcaatccag atgatttgaa aatcgatacg taccgagcaa gtggtgcagg tggtcagcac
    28081 atcaataaaa cagaatctgc tgtacggatc actcacttgc ctacaggaac agtagtagct
    28141 agtcaggcac aacgctcaca attgaaaaac cgtgagcaag cgatggggat gttaaaagct
    28201 aagctttatc aattagaagt ggaaaagaaa gaacaagaag cagctgcact tcgaggagaa
    28261 caaaaagaaa tcggctgggg ttcacagatc cgttcttatg ttttccatcc ctattctatg
    28321 gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc aagcagttat ggacggtgac
    28381 cttgatttat ttattgacgg ctatttaaaa atgcatctac aataagaacc cattgttaaa
    28441 ttgaaataaa attgtaagat tttgcaagca tgttgaaaca ttgccatgct ataatagcta
    28501 aagactgaaa aagatatgga gagaacgcca tgattgaaat gaaggatgta atgaagaagt
    28561 actcaaatgg tacgaccgct atccgcaatc tctcgattgt catcgaacaa ggagagtttg
    28621 tctatgtggt tggtccgtct ggagccggta aatctacatt catcaaattg atgtatcgag
    28681 aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca tgatctaata gcaatcaaaa
    28741 accgcgaagt tccttatcta cgcagagaaa tcggcgtggt gttccaagac tataaattat
    28801 taccaagaaa aacagtatat gaaaatgttg cgtacgctat gcaagttatc ggcagaaaac
    28861 ctcgagatat caaaaaacga gtaatggaag tgctggacct tgttggtttg aaacacaaag
    28921 ttcgagtctt tcctagcgaa ttatccggtg gggaacagca acgtgtctct attgctcggg
    28981 caatcgtgaa tacgcctaaa gtacttatcg cggatgaacc gacaggaaat ctggatccag
    29041 aaaactcatg ggaaatcatg aaattattag atcggatcaa tgcccaagga actactgtcg
    29101 tgatggctac acataacagt acgatcgtaa atacgattcg gcaccgagta attgctatcg
    29161 aaaacggccg cattattcga gatcaagcgg aaggggaata tggatacgat gattagaacc
    29221 tttttccgcc atttattaga aagtatcaag agtttgaaac gtaacggctg gatgacagtt
    29281 gcttcagtca gtgcggtaac gatcacgttg actttagtcg gtgtattcat ggcagttatc
    29341 atgaatgcaa ctaaattagc tcaggatata gaaggaaacg ttgatgtatc tgtattcgta
    29401 gatatcggta caaaagacga agagatgaaa aagctagaaa aagaattgaa tgacttagat
    29461 catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat tgaaaaaaat ccaaaacgaa
    29521 atgggtgatt cctggaaatt gtttgaaggt gacaataacc cgttatatga tgtgtatgta
    29581 gtaagtgcta ctgatccttc ttatacaaaa caagtagcag aagaagcagc agatttgaag
    29641 aatgtttctc gttctgatta cggtggagct tcctcagatc ggattttcca aatcgctggt
    29701 gcagttcgga catggggatt agttgcagcg gcattgctgc tgttcgtagc gatgtttctc
    29761 atttcgaaca cgattcggat cacgattttg tctagacagc gtgaaattca aatcatgcgt
    29821 ttagtcggtg caaaaaatgg atatattcgc tggccgtttt tccttgaagg tggctggatc
    29881 ggtctgatcg gtgcaattct tccaattttg atcatcacat ttggttatgc atggtttttc
    29941 caattgatca atccatcatt attacgttcc cattattcat tgattcatcc acaaaatatc
    30001 gtttggaaaa tcaatctttt aatggtcggc attggtgtga tcattggttc acttggctcg
    30061 atcatttcta tgagacgttt cttgaaattc taataccaca atataaataa aatgagaaag
    30121 ttggacagtt tcgttccagc tttctttttt tattggctag gagaatagaa ggaagcggct
    30181 tttttccctt tactttaaaa aacaacttgg gtattcttaa gttggtagaa gaagaggtga
    30241 agccgtgaaa attatcgttg acaatttttt tacgatcctt ttgattatga atattttgct
    30301 ttcctttatc atcgtttttc gagaaagaaa ggaaactgct caaacatggg catggctgct
    30361 cgttttgatg ttcattccag ttgtaggatt tattttatat atttttttag gaagagggat
    30421 ttccaaagag aaaatctttg atttgaagat ccaagggaaa atcggtaaga acctggaaat
    30481 tgaagaagac aaacaggcat tgatgcgagg gctttatcct catccgccta ctggacaagt
    30541 agatgtgaaa caattgatct atatgctcac ggtatttgaa agtacgctct atacgactaa
    30601 aaatgaaatc actttattta cagatggtcg tgaaaaattc gatgcgctga ttgaagatat
    30661 caagcaggca gaagaccata tccacttcca gtattatatt tatcgcagtg atgcattagg
    30721 tgaagaagtt cgagatgcat tgacagaggc ggcaagacga ggggtaaaag tcagagtcct
    30781 tcttgatgca tggggttcca cacaagtttc ttccagcttt tttcagaatt tgaaaaaagc
    30841 aggcggagaa gtcgcatttt tctttcctct atttgttccg tatattaatc ctcggatcaa
    30901 ttatcgcaat catcgtaaaa tcgtagttat tgatggggaa atcggatata caggtgggtt
    30961 caatgttgga aatgaatatc taggattggt aaagaaattt ggttattggc gagacaatca
    31021 tttgcgtatt tacggagaag cggtgtatac cttgcaaaac cgttttttga tggactggaa
    31081 ttctcagcat acaaaagaag taggctattc tcccaaattt tttccgtcca ttcacactac
    31141 tggtgatata gctgttcaaa tcgtgacaag cggaccagat accgaacatg aacaaatcaa
    31201 aatgacttat ttgaaaatga tttcgttagc caaacgggag atattgatcc aaacgcctta
    31261 ttacataccg gatggatcga ttcatgaagc actgaagttg gctcttcttt caggcgtaaa
    31321 agttcatatc caaataccta acaaaccaga tcatcttctc gtttactggg caacgtattc
    31381 tttcgctgca gagctgatcg agtatggagc aaggatcgaa acatatgaga atggatttat
    31441 tcatgcaaag acgatgatta tcgatggtgg cattgtttca gttggctcag caaatatcga
    31501 tgttcgttca tttcgcttgg actttgaggt caatacactg gtttatgatg aacgaatggc
    31561 ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt tctacgcatc tcacaaaaga
    31621 aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa ggattagcaa gattgatttc
    31681 gccgctcctt taaaacataa aaaggacatg agaaccatca acagatggag atctcgtgtc
    31741 cttttttaac ggggccattt ctgagagtac gtcagaaatg gagaaagcgt ctcgcatgag
    31801 cgaaattaac tttggggtat ctcttgtttt ttggggaaag caagaaataa gctaatatat
    31861 gctattaaaa gagacacatt tcaaagccag tatagc
//
LOCUS       DAYYHX010000008        31665 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000008 DAYYHX010000000
VERSION     DAYYHX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.008"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..883)
                     /locus_tag="ACHA6Y_000272"
     CDS             complement(35..883)
                     /locus_tag="ACHA6Y_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8986395.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1107..3047)
                     /gene="thrS"
                     /locus_tag="ACHA6Y_000273"
     CDS             complement(1107..3047)
                     /gene="thrS"
                     /locus_tag="ACHA6Y_000273"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM8986396.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3384..3563)
                     /locus_tag="ACHA6Y_000274"
     CDS             complement(3384..3563)
                     /locus_tag="ACHA6Y_000274"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM8986397.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3610..5433)
                     /locus_tag="ACHA6Y_000275"
     CDS             complement(3610..5433)
                     /locus_tag="ACHA6Y_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8986398.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5711..7990
                     /gene="helD"
                     /locus_tag="ACHA6Y_000276"
     CDS             5711..7990
                     /gene="helD"
                     /locus_tag="ACHA6Y_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM8986399.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8083..8730)
                     /locus_tag="ACHA6Y_000277"
     CDS             complement(8083..8730)
                     /locus_tag="ACHA6Y_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986400.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8909..9628)
                     /locus_tag="ACHA6Y_000278"
     CDS             complement(8909..9628)
                     /locus_tag="ACHA6Y_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8986401.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9759..10910)
                     /locus_tag="ACHA6Y_000279"
     CDS             complement(9759..10910)
                     /locus_tag="ACHA6Y_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM8986402.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11127..11867)
                     /locus_tag="ACHA6Y_000280"
     CDS             complement(11127..11867)
                     /locus_tag="ACHA6Y_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8986403.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11871..12194)
                     /gene="rsfS"
                     /locus_tag="ACHA6Y_000281"
     CDS             complement(11871..12194)
                     /gene="rsfS"
                     /locus_tag="ACHA6Y_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM8986404.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12251..12844)
                     /gene="yqeK"
                     /locus_tag="ACHA6Y_000282"
     CDS             complement(12251..12844)
                     /gene="yqeK"
                     /locus_tag="ACHA6Y_000282"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM8986405.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12828..13478)
                     /locus_tag="ACHA6Y_000283"
     CDS             complement(12828..13478)
                     /locus_tag="ACHA6Y_000283"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM8986406.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13488..13799)
                     /gene="yhbY"
                     /locus_tag="ACHA6Y_000284"
     CDS             complement(13488..13799)
                     /gene="yhbY"
                     /locus_tag="ACHA6Y_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM8986407.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13815..14924)
                     /gene="yqeH"
                     /locus_tag="ACHA6Y_000285"
     CDS             complement(13815..14924)
                     /gene="yqeH"
                     /locus_tag="ACHA6Y_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM8986408.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14921..15448)
                     /locus_tag="ACHA6Y_000286"
     CDS             complement(14921..15448)
                     /locus_tag="ACHA6Y_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM8986409.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15628..16413)
                     /locus_tag="ACHA6Y_000287"
     CDS             complement(15628..16413)
                     /locus_tag="ACHA6Y_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM8986410.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16406..17275)
                     /gene="accD"
                     /locus_tag="ACHA6Y_000288"
     CDS             complement(16406..17275)
                     /gene="accD"
                     /locus_tag="ACHA6Y_000288"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM8986411.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17276..18655)
                     /locus_tag="ACHA6Y_000289"
     CDS             complement(17276..18655)
                     /locus_tag="ACHA6Y_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM8986412.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18663..19082)
                     /gene="fabZ"
                     /locus_tag="ACHA6Y_000290"
     CDS             complement(18663..19082)
                     /gene="fabZ"
                     /locus_tag="ACHA6Y_000290"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8986413.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19107..19583)
                     /gene="accB"
                     /locus_tag="ACHA6Y_000291"
     CDS             complement(19107..19583)
                     /gene="accB"
                     /locus_tag="ACHA6Y_000291"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM8986414.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19586..20821)
                     /gene="fabF"
                     /locus_tag="ACHA6Y_000292"
     CDS             complement(19586..20821)
                     /gene="fabF"
                     /locus_tag="ACHA6Y_000292"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM8986415.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20840..21577)
                     /gene="fabG"
                     /locus_tag="ACHA6Y_000293"
     CDS             complement(20840..21577)
                     /gene="fabG"
                     /locus_tag="ACHA6Y_000293"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM8986416.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21589..22506)
                     /gene="fabD"
                     /locus_tag="ACHA6Y_000294"
     CDS             complement(21589..22506)
                     /gene="fabD"
                     /locus_tag="ACHA6Y_000294"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8986417.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22613..22837)
                     /locus_tag="ACHA6Y_000295"
     CDS             complement(22613..22837)
                     /locus_tag="ACHA6Y_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8986418.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22890..23852)
                     /locus_tag="ACHA6Y_000296"
     CDS             complement(22890..23852)
                     /locus_tag="ACHA6Y_000296"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM8986419.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23862..24302)
                     /locus_tag="ACHA6Y_000297"
     CDS             complement(23862..24302)
                     /locus_tag="ACHA6Y_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8986420.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24608..25573
                     /locus_tag="ACHA6Y_000298"
     CDS             24608..25573
                     /locus_tag="ACHA6Y_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8986421.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25630..25860)
                     /locus_tag="ACHA6Y_000299"
     CDS             complement(25630..25860)
                     /locus_tag="ACHA6Y_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM8986422.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25946..26833)
                     /locus_tag="ACHA6Y_000300"
     CDS             complement(25946..26833)
                     /locus_tag="ACHA6Y_000300"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8986423.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26922..28145)
                     /locus_tag="ACHA6Y_000301"
     CDS             complement(26922..28145)
                     /locus_tag="ACHA6Y_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8986424.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28162..29208)
                     /locus_tag="ACHA6Y_000302"
     CDS             complement(28162..29208)
                     /locus_tag="ACHA6Y_000302"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8986425.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29372..31099)
                     /gene="ptsP"
                     /locus_tag="ACHA6Y_000303"
     CDS             complement(29372..31099)
                     /gene="ptsP"
                     /locus_tag="ACHA6Y_000303"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM8986426.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31099..31365)
                     /locus_tag="ACHA6Y_000304"
     CDS             complement(31099..31365)
                     /locus_tag="ACHA6Y_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM8986427.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
BASE COUNT         8733 a         6944 c         5443 g        10545 t
ORIGIN      
        1 ttctaaaaaa cttatataaa aaatagtaag gtttttattt cgaatccata aaatattctt
       61 ggattgcttc tcgtgttttt ttcacttttt cgctaagctt ttcgttttta gccatataga
      121 aataaaaaat gatatcgatc gtagcaaatt gggccaaaag tgaattagtt gccgcacttc
      181 gattcgtgat ttcttttggt ctagatgttt gtacgagtac atcggctatc ttgctaagcg
      241 ggttatttcc aaattgcgtc aacgcaatga tttcgatatt catgcttttc gccagctcgg
      301 ctaatgcgac tacatctgca cttctacctg agtttgaaac aagccaaagc actttctttt
      361 tctcattgct gacaagttgc gggagcaaga cgtggatatc tttttcaaaa atgattggct
      421 taccgacacg agaccatttt tgcagaatat cttctactac taaagaagag gcgccaaccc
      481 catataaaaa aacagtctct gccgattcca aacactctac gactttttgg atcgtttctt
      541 cttctagaat atctactgtt tcttttatcg ctacttgtgc attgttacca agtttgtttt
      601 tgatagagta aaaggactca tttgcttcta tatcactaaa accttcatcg attccagtct
      661 gtagttctgt tgaaatcgtg attttgaagt ccgtcagact ttgaaaaccg atttttttgg
      721 aaaaacgtac aactgtcggc gcactcaacc cggaagcttc tgctaattca ttagctgtca
      781 ttgtagggac cttctcaata ttaccgataa tgtagttggc caactttttt tctgctgaag
      841 aataatcttc catcatactt tcaatacgac taattacgct cataagttcc tccacccttt
      901 taatgatagt ctatgtaagt ataccatatc agacccttat tcacggtcgt atactggata
      961 aaaaacaaaa aaacaatccg cccaacagaa tatcttatcc catcaatccc acagattcta
     1021 ttgagcatct gtgtctttga ggaatacctg ttatctattg ttcggattgc tttctatcta
     1081 ctactttttt acaggaaatt tacctattag ttttctctgc tgtaattttt gacttctgct
     1141 tccatagcat caataaagat attcaaatct tctacagctg tttctttact tccataacga
     1201 cgtacgttca ctgttgcatc ttccaattcc ttgtctccaa caactaattg gtaagggatc
     1261 ttttgtgttt gagaggcacg tatcttatag cccatttttt cattacgatc atcaacttct
     1321 acacgcaacc ccttcatttg caaacgttct ttgatttcat aagcataatc gccatgtgca
     1381 tcaacagaaa cagggatgat tgttgcttgt ataggtgcca accatgttgg aaaagcgcct
     1441 ttgtatactt ctgttaaata agcgacaaaa cgttccattg tagaaacaat tccgcggtgg
     1501 ataactactg gacgatgcgt attttccccg tcttctccaa catatgtcaa atcaaagcgc
     1561 tctggcaata agaagtctaa ttgtatagtt gataatgttt cttctgtacc aagagctgtt
     1621 ttgacttgaa catctagttt tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa
     1681 tctagttcta attcatccat agcggctttc agcattgctt gggctttttc ccacattgcg
     1741 tcatcatcaa aatatttatc tgtattgttt ggatcgcgat agctcaagcg gaaacgataa
     1801 tctgtaatat tgaagtcagc atatactgcc accatcaatt caagcgtacg tttaaattca
     1861 tctttgattt gatcaggacg aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca
     1921 cgttgtaaac ctgacaaagc acctgatttt tcatagcgat gcatcattcc taattcagcg
     1981 atacgaattg gtaattcacg ataagaatgg atcgtgtttt tgtagaccat catgtgatga
     2041 gggcagttca ttgggcgaag aacaagcatt tcgccatctc ccatatccat tggcgggaac
     2101 atgtcttctt gataatgatc ccagtgtcca gatgttttgt acaactcaac atctcccatt
     2161 attggtgtat atacatgttg gtaacctaga ctaacttctt tgtctacgat ataacgttcg
     2221 atcgtgcgtc ggattgttgc cccttttgga agccagaatg gcaaaccaga acctacttct
     2281 ggggaaacca tgaacaagtc aagttctttt cctagtttac gatggtctct ttcttttgct
     2341 tcttcacgga gacggatata ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat
     2401 acacgttgca tcatttgatt gtttgagttt ccacgccaat aagcacctgc cactgataat
     2461 aatttaaata cttgtatacg gccagtggat ggaacatgtg gtccacgaca caaatcgatg
     2521 aattcgcctt gttgataagc agtaatcact tcttcttctg gcaattcact aatcagttct
     2581 actttgtacg gatcacttgc aaaaatttct aatgcttcct gttttgaaag aacacggcgt
     2641 tcgatcggca gattttcttt gacgattttc atcatttctg cttcgattgc cggcaaatct
     2701 tctgcagtta cttggttttg tccattatct gtatcgtagt aaaaccctga atcgatagct
     2761 ggtccaacgc caaagtggat atttggatag aggcgacgca tggcttgggc catcaaatga
     2821 gctgctgaat gacggactaa gccaagtgca tcttcatggt ctggtgtgat gatctcaata
     2881 cttgcatctt cctcgattgg acgagtcaaa tcaacgagtt ttccgttcac tttaccagct
     2941 aaagcttttt ttgctaagct ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca
     3001 gactcaaatt ctttgactgc accatctgga aaagtaattt ttatcataat agtttcctcc
     3061 ttgaaatgtt ttgattgatc agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct
     3121 tgaaaagaga tactaggacg aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg
     3181 ttcctcaagc gttgtaacgg tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc
     3241 atcctttggt tttctcctgg aaaagtttca gcctagcttt tcctctctta tcgaagactc
     3301 tcaaattcag ttggctttct catcaatcaa ttatgtgttt atttaaccac taataaatag
     3361 aaaaatcaag tgggaaacta aacttatttt ttccattccc catttacacc gtatgttcct
     3421 tttttcatct cattgatgat gacatggatg tgttcttttg gtgcctgcgt gtttttatga
     3481 acagcttccg tgatatcttt catcatattt tctaattgtt ccggactgcg accttctact
     3541 aattcgacat gtacgaatgg cataatgctc tctcctcttt ctgatcgttc ttttttcaac
     3601 tcgattctat catactatgt ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt
     3661 ttttgctctc aaggcactca aaatagagac cgtatccacg acttcttgca aaacagctcc
     3721 gaataatgcg gggattagtc ccgtacttgc aattaacatc aaaaaaatac aaataaagat
     3781 gccaatcagt acagactgtt tcgctattct catcgtatct cttgaaatca ctactgcttt
     3841 tgctacacgc aagagatcat ctttcaagat gaccgcatct gcactttcac tagcagctgt
     3901 agaaccatga gcccccattg caataccgat atctgcagca gcaagtgctg gtgcgtcatt
     3961 cacgccgtct cctaccatcg caacaggtct ttgatcttta gggatacttt ctaaaatggt
     4021 cagtttatct tgaggcaagc tacgggcatg tacagaatct aattggactt tttctgcaat
     4081 cgtttgagca atggcagatc catccccggt gatcatcatc aaatttttca catcaagttt
     4141 tcttagctcc ttgattgtct ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt
     4201 tcctgcaaaa gatcgatcaa ttgttacata gattgctgta ttggacgttt ctatttcttc
     4261 agctgaaaca aaagaggcac gtccaatctt tacttcatga ttattgacta ctgcttggat
     4321 tccttgaccg gtcacttctt tcagttgact aacaggatac agttggctgt ttttcacgtt
     4381 gttcactaac gaacgagcta agatatggct agattcctgt tctgcactag ctgcatacag
     4441 aagtagctct tcttttttta ttccattgac tggctgaatc gcctctactt ccaatatccc
     4501 tttggtaagc gttcctgttt tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat
     4561 cgttgttcca gtttttacaa cgatcccgtt tttacttgaa cggctcattc ctgcaactaa
     4621 agcaatgggc gcagctagaa tcagtggaca aggtgatgcc actaccagta cctcagcaaa
     4681 tcgtaaaggg tcttttgaga accaccaaga aataccaccg atcaaataag caatcaacgt
     4741 gaaagggact gcataacgat cagctaaacg gacaaatcgg gcaggctttt cttttgattc
     4801 ttcgactagt ttaataatca tttgatactg gctgtctgcg gcacgttttt cagcgatcat
     4861 ttggatcgat ccgtctccat ttaaagaccc tgacataatc tgatctccag gtttcttggt
     4921 ttccgggcgg gactcacctg tcaaagaaga ttcatcgacc gaagattctc ctttggtcac
     4981 tcgaccatct gcgggtacaa gttcgccagg cttgactagt aactggtcat caatatttat
     5041 ttcatcgatt actatatcct gcaattttcc ttctttgaat cgatgggcaa ttcttggggt
     5101 gttatctaac aaagactgca gttctttacc cgctcgttta ttcgcgtaat cctctagact
     5161 atctccgccc gtaagcatga tcaatatcat caaactagcc cagtattctt tgacggctaa
     5221 tgttgcaagg attgcggtaa tcgctaatac atctacgcca tatcttcctg aacgtaacgt
     5281 cttgatcatt tccaaaaaca tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc
     5341 aaatgcaagg gccggctggt gaaaaacaaa ttcacttaat aaagctatcc caccaataag
     5401 caaagtaagt aaaaattttt tgaaatgtgt cattgttttt tctcctcttt tatgtgactt
     5461 agagaggtta gtaataaatt tccagctgcc tctttcatct taagcatagt ccttgactag
     5521 cactttttaa aacaataggc cttaattgaa aacaactatc actatcagcg tttccaaaat
     5581 tcatcagttc tacaaacaaa aatattcagc aaggattact ttttcgattc atcagttaaa
     5641 aagtaccttt tttcgaaaaa ctatgctata cttttaattc gttatttacg gcggaaggag
     5701 caaacatgcg atgaatgaga aacaactaga acaaacgcac gtggatgaaa cgatcgaact
     5761 aatccagatc gaacaaaata tgctggatac aaaacaacaa gaattgactc aggaaatgaa
     5821 tcattcgaca aaagacacag ctaatcacaa gattcgagct ggttccaatg aagcttttta
     5881 tgaatctgct gttgaatatc gccaacatga gcaggaattg cttttgaaat accatactct
     5941 tgaagcacag caaaaacgcc tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc
     6001 ccgtatcgac tttaaagaag accgtgaaac agaaacatta tatctaggta tcgcttcttt
     6061 aagagatctg gcagaagaaa caatcgttat tgactggcgc gcaccaattg ccaatcttta
     6121 ttatgaaggt gagattggtc ccgcatttta tgaaactgaa acagatagaa tagatgtcga
     6181 tctgcagttg aaacgacaat ttaaaatcca agaaggacgt attttatcta tggtagatac
     6241 gtcggaagta atcaatgatg actttttgct agagatattg gatgaatcat caagcaacca
     6301 tatgaaaaac atcgtctcta cgatacaaaa agcacaaaat gcaatcattc gggacacaac
     6361 aagtaaagta atgctgattg aaggaatcgc cggaagtgga aagacatccg ctttgcttca
     6421 acgtattgct tttctgttgt atcataatcg caagtggctc gaggctgaaa acgttcttct
     6481 attttcaccc aaccatttgt tttctgatta catctcgact gtattgcctt cattaggtga
     6541 aagcggcgtg cctacacaga cattcaaaaa ttatttggtg cagctgctgc cagagttttc
     6601 tttactagat gaacagcaac aagaaagtgg ttttttgcta ggggaaaaag atccgataca
     6661 agtgatgaaa agcggattga ccttagtcga tcagattgat cgctatatcc aaggcatcac
     6721 aaactacggt cccttgttcc gtgatatgaa aataaatggc cgtacgatcc tatcaaagga
     6781 atcgattcgc caatggtata aagaaacaaa tgaacaatta cctttacatc atagactttc
     6841 tctattacaa acgaagctcc tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca
     6901 gcaatgggtc aaagatctgg cggaagaaca gctccaagaa ctatacgcaa cagatccaaa
     6961 tcttgaatat acagaacaaa aggagcgttc attacgtaaa aaactggcca accaaatcgt
     7021 acgtaaaaga ttccgtcgaa tccatcgagg gatcatgaat tatcaatttg tcaatataac
     7081 gaaacaatat ctccactttc tacaaactgt gccactttct cttctaacac gacataaaat
     7141 cagcgagacc aggtggcagg aacatttgac aactgttcgt acacacttgc gtcaaagaga
     7201 attgcgtcaa gaggatgctg tactattctt tttactgatg cgccgaacat atccagtgac
     7261 ggttacacac aaagctcgct atatctttat tgatgaaatg caggatttcg caccatcaca
     7321 agtggcattg ctgcaagcct tgttcccaaa agcaaatttg actttctgtg gagatctaaa
     7381 tcaaaatgtc tttggaaatg aaacgatcgt tggatcattg gaagaccttt tcgtggaccg
     7441 tgaagtcact cgtttccaat taacgacaag ctatcgctcc acaaaagaaa tcactgattt
     7501 tgctaatcat tttattgtga aagaacatca agtagaaaca acagcaagaa aaggagacct
     7561 tcctttgctc atccaaggaa caacagatga tatcaaattg aattggttag cagataccat
     7621 tcgaacgact cagggaaaag cccggtattg gcgtacagca atcatagcta aaacacaaga
     7681 agaatgcgaa gctctttatg ctcaactgcc agacgagttg aaacaacaag tccagctgat
     7741 tttaaatgag agtgacttta tgaagcgttc cattatttta cttccagcct ttttagcaaa
     7801 aggtctggag tttgaccgag ttttcttgtg ggatgtagat gcagaacatt tttcttctca
     7861 gcaagacaaa ttgatccttt acaccatgtg tacacgtgcg atgcatgaat tgaccctttt
     7921 tacctccaat aatgaaagtc ctttgttaaa caatatggat atttcatctt atgagaaaaa
     7981 agtactgtaa gtaaaaagac aactgctatc attaatgtta gcagttgtct ttttatttac
     8041 aataataatt gcagaaaaag taattcaatc atttaaggtc atttattcta tatataaatc
     8101 caaatctgtt tcaggatatt ttaatttgac aagcgaaata atcattttca gttcttccat
     8161 gttttctgga tattctgggt caaatacctt gctatgacgg ccaatcccgc cattgtcgat
     8221 caatcggtaa attcgattcc attcaatgaa ttggtcaatt gattggaaat tgctgattgg
     8281 atagccgcca caatccaaaa tctctgatac acgaaatgct aggtatgcac tatcgatcac
     8341 tttatttttt ttcgtgtcta tcttctcatt tctttcgata agaatttttt caggacgatg
     8401 tttcaagctc cactctaata gtagaaagaa agcctccatc cgatactctt cttcaaataa
     8461 ccgaaaggct gaatcagaac gacgaaaaag cagcggaatc agataatttt gtctataaat
     8521 cagcattggt gcgcggtcca ccatacactc taaataacgg ttcagatacc ctgtaccgac
     8581 atagtttcct aatcgatagc cttcaacgcc gaatcttgcg tatttttgcg ctaattttga
     8641 acaatcagtt acgttcacag ctaactcttc tactttgcgg taaccgctta agatacgttc
     8701 aaaaggttct aaagtgattt ttgcttccat attcttcccc gtccatcttc cacatacttc
     8761 atatcatttc acatgatact tatgctctca tcataccaca ggttgaatta gaaaagaaaa
     8821 taaatcacac aaaaaagcaa gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga
     8881 ctgaatactt ttcgttaaaa ctggttgctt agtcttctaa tgccacatta tggaacacat
     8941 gctgtacatc ttctagatct tccagtgcat caatcatttt ttcaaatgtt tcttgatctt
     9001 ctggagatag tgttacctcg ttttgaggga tcatttggat ctcagcgatt gaaaaatcag
     9061 tgattccttt ttcttttaat gcttcttgta tactatggaa atccgctact tcaccgtaaa
     9121 cgatgatttg tccttcttct tccattacat cgcgaacatc gatgtctttt tccattaggt
     9181 attcaaggat ctcgtcagcg tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca
     9241 tgtaagaaac cgctccggaa acacccatgt tcccgccatt tttcccaaat gctgcccgta
     9301 cgtctgctgc tgttcgattc acattgttcg tcaatgtatc aacaatcacc attgagccgc
     9361 ttggtccaaa tccttcatag cgcaattcag agtatgtttc atctcctgca ccttttgctt
     9421 tttcgattgc tcggtcaata atatgttttg gtacattata agttttagcg cgatcaatga
     9481 cgaaacgtag tttttggttt gcatgcggat caggatcacc tgattttgct gccgcgtaga
     9541 tttcaatccc aaattttgca taaatccggc tattattagc atctttagca gttttctttg
     9601 ctacgatatt tgcccattta cgtcccatat tttcacttcg ctttctgatt tgatagtttc
     9661 ataacgcaaa aacgtcctgt tccattatac ttgtgaatca cgattctgtg aagctttttt
     9721 tctttcttcc ttcattcttc tcatttttag tcaattatct atactcggat tgggatttgc
     9781 ccgaaattct gttccggaat attgcctccc aattgataga tttgatcact tctaactaac
     9841 agttccgctt cttgtgcctc tttttttccg attcgggaat aaatagaaat ctcttttttt
     9901 tcttttaaga atcttcttcc agcatcggta aatccaagta gtcgaagata atttttattt
     9961 tgttggacag gaatctcttg ctttttgata ttcagcaaga cataacaaga taatcgttgg
    10021 atccgtgtcc atgtatatct ttttgttttt acctttgaca caaaatcctc aaaactatca
    10081 gcagttgcag ctgctgtttt catgcgcgat tctattcctt cggtcatctg atagatatct
    10141 gtcagttcca gcaaagtaga agataacaac cgataacgca aatacggcca ataagattcc
    10201 caagtctgca gcgaacaagt tgaaagatct tcttctgtct gaggcggcac aagtccagct
    10261 attctttttc cttcttctaa cgcatggcga atggctgttg cactggctat cctctgatct
    10321 gtgatgattt tttcatgata gcctgcactt ttacgtcgga tcggatgtaa ttccataggg
    10381 tggacatact tggcattttc ctttgcataa cttaatccca agatatgatt tggcgaagaa
    10441 aaatcaagcg taatatcggg atacagactg cgaaaaacct cagtcatttt ttgcggataa
    10501 ctatacgttt gatcagacag ttcttgaaat gcctcttcta tcctatcttg attctcttgg
    10561 acaaagcgtc cgaaactgct gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa
    10621 ctgtcacagt ccagtgtttg caagattttt actgcacctt tagcaaaata gtcagctgac
    10681 tgaaccgccc attcgacggg aagctccact actaaatcca caccattttg taaagcttca
    10741 ttcgcgcgat gccatttatc gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc
    10801 attacagcaa tgacgacctc tgctcctgtt atttttcgtg ccatttccac atgatagcga
    10861 tgtccattgt gaaaaggatt gtactctacg ataatcccgc agcttttcat gcgcatcctc
    10921 cttcatagtt atcgataaaa aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa
    10981 aatacccgaa ctatataaac tattctcgca aacacaatac ttctgcgacg aaaagctgca
    11041 ctgcaagagc aatgcctata tcgttcggat atataattta tgttgaagat ttattctttt
    11101 cttgttaact cgagcaggca aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt
    11161 gatttctctg atgctgcttc gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg
    11221 ctgttttcca gcattcttaa ataactttcc aatggatagg tacgctcttg atgtagttca
    11281 tcttcacgaa taaatcgacc agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt
    11341 tcgatgctgt gtttctcgct tcctggataa ctatcccaca aaaaagcaaa attatcccct
    11401 tgataatgat agctatattc tggaaaaacc tcatctactt gataggtgga gtgtacgtca
    11461 aataaaaatc ggccattttc ttctaacgct tgatagactt catcaaacac ttgctgtacc
    11521 tcttgtctat ttttcatata gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt
    11581 ccgatttctg ataaatcgag catattgcct tccacaaatt ggatcgatgc gtcttcttca
    11641 tatgctcgct ggctggcaat catcaacatc tcttctgaaa ggtctaaccc tgtcacttta
    11701 tatccatctt ttgcaaaggc aaccgctaat gctccagtac cgcatgccag ttccaaaata
    11761 ttttttgtgt cttcttgtaa atgacgtttc gaaaaagcta accattgctg ataaagtgtt
    11821 tcgtccatca cttcatcgta aatgaaagca aatgtttcat aagccatatg ttaatccaac
    11881 cattcactaa gatcgactag cggagcatct gaccatagtt tttccagatt atagaaattt
    11941 cgttctggcg catggaaaac atgtacgatc acatctccaa gatcgatcaa tacccatttt
    12001 ccgccttctt ttccttcaat acgttttact tcatatttgt tttcttcttc tttatcaatg
    12061 atttcttctg taattgcgtt gatttgacgt tcgctatttg ctgaacagat catgaaataa
    12121 tctgccaaca aggaaacatt tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa
    12181 tctgctgctt caatcgcaat ttttaattgt tctttactat ctatgatgat ttcctcctaa
    12241 tatgagcagc ttattttttt gctacccagt aattatatgt ttcgattgtt tttggataaa
    12301 tctgttgttc ttgcgcaagc aaatgggcca atgtatgttt tgtttcaaat gctacagctt
    12361 cgtctagatc agcccatgca atggctcgtg catcttgtac cccaggaaaa tctcgacctg
    12421 gttcaatata gtctgctaca taaatgattt tgtcaaggag actcatctta gcagccccag
    12481 tcgtatgcac ccggattgca tgaagaatct ctgcatcggt gattcctaat tcccgctcta
    12541 caaacgaagc gccaacaaga ccatgccaga ttgcattatt ataattcaat aactccggat
    12601 caaaaccgtc tcttacgatt accattttga actcctcatc tggtcgttct tttgcataat
    12661 catgtgtcaa tgcagcaata ctagcttttt caggagaagc accgtatttt ttggcaagag
    12721 ccacggctgt ttcttctact ccaagcacat gcttgaagcg ttgttcgctc attctcatct
    12781 gtattttttg catcaggttt tctctagaat aagcagtata ttcattacta tagtccatat
    12841 tcatacaacc cttcattttg aatataatca attactttat ctggaacaag gtagcggata
    12901 gaacagcctt ctttgatctt ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca
    12961 tctacccaaa ttacaggata tggagtatcc gttgtgtatc ctggtcgacg tatacccaca
    13021 aagttgacca tagaagtcag ctcatcgatt ttataccatt ttggcaaata ttctaccata
    13081 tccccaccga taataaagta ataatctgta tccggattgt tttgcgtcaa ttccttcata
    13141 gtatcatagg tgtaactttt cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa
    13201 ggattgtctt ccaccgctaa ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt
    13261 ttctcatcaa catgaggtgg ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa
    13321 ttccggccag cttgttcagc catcactaaa tgtgcaaggt gaacgggatt gaatgttccg
    13381 cccaagatcc ctacttgctt tttcttctct aagaattgag gcatttcttc aggaaataat
    13441 tgtgaacgaa caaatgtctt agcttgcgta ttcatcatgg tcactcctta tattgctttt
    13501 acttcttttg atagcctttg atacttttct tttgtggaag gtttgtataa tacaataaca
    13561 cgtccaatga tttggacgat ttcgcaatct atttcttttt ctaatacttg agctgcttct
    13621 tctgcaatct catctgtatt ttgaagtaac gatactttga tcagttctct tttttctaaa
    13681 gcttcttcaa tttgaacgat cattgctgaa ttgatcccac cttttccgat ttgaaaaatc
    13741 ggctgaagat gatgcgcttt gctacgtaaa aaacgctttt gttttcctct taaactcata
    13801 attttccttc ttccttaaat caatgctttg cgtttcaata catctacacc ctttggcgcc
    13861 cagcccgcaa ttactactgg cgctgttaca gtgatccagc ctagaccggc aaaaacgatg
    13921 tctgtttttt ctttcacaga aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca
    13981 tcaggacgag gaggctgtaa taatccacct acatgttttt gataaaattc gtctgctttt
    14041 tccagtttcg ttcgatgaat atttacatca tttgacacat aagcaataaa tgaagcacga
    14101 ttgccgctga taaaatcaaa tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc
    14161 agctgataaa ctttaggttt gatttctttt aacggtgcaa tgatccgcaa atctttttta
    14221 cctaagtagt gcgccatttg atggcgatgg ataattcccg gtgtatcaat caaaaaatgc
    14281 ccgtcatcta aaggaatctc gattttatcc aaggtcgttc ctggaaactg cgaagttgtg
    14341 attacatctt gtactcccgc tgtatttttg atgatttgat tgataagcgt cgattttccg
    14401 acatttgtta ctccgacgat atagacatcc cgtccatcgc ggtattcttc gattttttct
    14461 aatagttccg tcatctcatg agatttctta gcactagtta ataatacatc tactggacga
    14521 attcctgctt cgtaagcacg ttccttcatc cattgtgtta atttagtttt tttcaatgat
    14581 ttaggtaata tatccacttt gttcccaact aaaagtactg gattgtcacc gacaaaacgg
    14641 tgtagtcctg ggattagtga accattgaag tcaaaaatat ccaccacatt gacgatcaat
    14701 gcatcttttt gccctaattc attcagtaag cgcaaaaagt cgtcatccgt caatgagaca
    14761 tcttgggtct cattataatg gcgtaatctg aaacaacgct gacaatagac ttccccggaa
    14821 gctaaacctt tttccaaagc cgtttgcggc gtgtatccta attcaccggg tttgtttgtt
    14881 tgtataacgg caccacagcc gatacattgt aattcttctg tcattcgatt ccacctcgcc
    14941 agcccatatc agggtgtttt gctaatagat aggccataat tcttttttcc caaaaacgat
    15001 taaattgtgt cttccaacta tctgttgcga taatgggttt tacaaggatg ctacgtatcc
    15061 ctgcacgatt ggctccacgt atatcagtca taatctgatc gcctaccatt actatctcat
    15121 catctgctaa atccaattgt ttctttgcta tgttgatccc tcttgctaaa ggtttcattg
    15181 ctcgtgcaac atacaaaaga tcaaattttt caatcgctcg cgcgacacgt tttgaattat
    15241 tgtttgaaac aacaacaaca gggattcctg catttttcat ttctaaaatc caagtaagca
    15301 actcttctgt tccatctgga ttattccatg caatcaacgt attgtctaaa tcggtcaata
    15361 ctgctttgat gcctagattc tttaattggg caggtgtaat ctgatagatg gcttctacca
    15421 tccatgttgg tttgtaattt gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc
    15481 acataaagta ctcctcaacc ccgtattata ctgcatatca gcaaaaaaaa ctacctctat
    15541 actcgttcgt ctttcagaaa aagcatttct ttacaaaatg accaaaaata aagattgatc
    15601 acagaaaaat ttagaaacaa aaaacaacta atattggcgg aacctcttgt atcgtttttc
    15661 cagtaattta tgctgatcta atcgaacaag ttccgtcaat ctttcaatca acgctttttt
    15721 cataatgtgt gcgactttat atcgtggcaa ctgctcgccg ttgaacgttt caggaataac
    15781 tcgatcaacg attcctaact cgcgtagttc tggcgcagta attttcatca actcagctgc
    15841 ttctgcggcg cgactaccgt ctttccacaa aatcgacgca aatccttctg gagaaagcac
    15901 agcataaatc gtttcttcca gcatccagac ttcatccgct aaacctagag caagggcacc
    15961 gccacttcca ccttcaccaa tgatgatggc gatgatcggt acctttaaat cagacatttc
    16021 gagcagattt ctggcaatag cctctccttc gccgcgctct tctgctccta caccgcaata
    16081 tgcacctgct gtattgatca aggtaatcac cggtctattg aacttttccg cttgtttcat
    16141 cagtcgtaaa gcttttcgat agccttcagg atggggagaa ccaaaatttc tgcggatatt
    16201 ttcctgcaga tcccgtcctt tttgaatacc gatcactgtt accggtttgc catctaatcg
    16261 agcgattcca ccaacaatcg cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc
    16321 gaaagaatcg aagatctctt caaaaaaatc caagcttgtc catcgttctt tagctcttgc
    16381 taactgaacg acatcatgtg ctgtcttatc cacgatctat ccatcctttc tgtgtatgca
    16441 attcaagaag cgtatggatt ttctgtttca attccatgcg gggaacaatc tgatcgacaa
    16501 aaccatgaga taataagaat tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg
    16561 tctgttcgat gacgcgtctt ccagcaaaac cgatcaagct ttggggttct gctagaataa
    16621 tatcgccttc cattgcaaaa ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag
    16681 ttagataaaa aagaccttca ttgctatgcc gtttgacagc agcagagatt tttgccatct
    16741 gcattagaga aaagattcct tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga
    16801 ctggaagact ttcctttgtt gctcgttcga ataatctggt gatcttttct ccgacaatcg
    16861 ttcccatact tcccatgata aaattaggat ccatcactcc aataatgaag ggaatcccct
    16921 gaatggttgc tttaccagtg aggactgctt catgaagtcc tgttttatct tgcatttttt
    16981 taattttttc cgtatattcg ggaaatgcta gcggatcttt ggttacaaga tccgtatccc
    17041 attcttcaaa acttttttca tcaataatca aagctagacg ctgccatgca ccgattcgaa
    17101 aagcgtatcc gcaatgaggg catacttttt cttcaccgat gtctttcgta taaaggatat
    17161 gtttgcaatt tggacacttt gcccacatgt tatcaggaac agatggcgag ttagcaggtt
    17221 tgctctctct attaggattg atccgaatat atttcttttt cttaaacaga gccatctatg
    17281 cgcctcctat ctccgtttct ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg
    17341 tgctgtaatc acctgctatg actgcaggat gactaatcaa atccatctga aattctgcgt
    17401 tcgtgatgat tccttctgtg ataaattcac ttaaggctcg ctgcatcttc attaatgctt
    17461 caaaacgcgt atttccatgg acgattattt ttgcaatcat tgaatcataa tatggcggga
    17521 tcgtatatcc cgggtaaact gcactgtcta ctcgcagtcc catcccacct gctggaagca
    17581 gtaagttttg gatcttccct ggagaaggag caaaatggaa agcaggattt tctgcattaa
    17641 tccggcattc gattgcgtgt ccttggaata ctacttcttc ttgacacaca ttcaaaggct
    17701 caccggctgc tatctccacc tgttttttta ctaaatcgat tcctgtgacc atttctgtta
    17761 ctggatgttc cacttggatt cgagtattca tttccatgaa ataaaaatcg ccttcttcat
    17821 ccatcagaaa ctcgatcgtt ccggcatttt cataattgac tgctttagct gctcgaacag
    17881 cagcgtctcc tagtgcttct ctttttgttt gtgagatcac tacagaagga gattcttcca
    17941 gtactttttg attgttgcgt tgtaaagaac aatctcgctc gcctaaatga atgacatgac
    18001 caaatcgatc acctaagatc tgaacttcta tatggcgagc aggataaatg attttttcca
    18061 aatacatatc atcatttcca aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct
    18121 gtgggagctc ttcttttgat tgaactttcc gaatgccttt cccgccacca cctgcagctg
    18181 ctttcagcat cactggataa ccaattctgt ctgctatttc caatgcttct tctactgtag
    18241 ataatacacc agtacttcct ggaatgactg ggacatttgc ttcttgcatc aattgacgcg
    18301 cattgatctt atttcccatt gcatcgatcg ttgcactttt aggaccaata aatgtgatat
    18361 tgcactcttc acacatggag gcaaatcggc tattttcaga taaaaaacca aatcctggat
    18421 gaattgcttc ggcttttgtc acgattgcag cgctcagtac ttcctgaaca ttcagatatg
    18481 aatcaacggc ttttgccggg ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg
    18541 cttcggcatc tgcttctgaa tatatcgcta cggtttggat ccctagttcg cggcatgcac
    18601 gaatgatacg tacagcgatt tcaccgcggt tagctatcaa tactttagaa aacatatttc
    18661 acctatccaa tcataaaggt taattccgct tcggctactt ttttaccgtc tactttagcg
    18721 attcctttgc ctattccggc tgaagctttc actttcaata tctcaacttc aaggtataag
    18781 gtatctcccg gtgtaacttt cttacggaat tttgctttgt ccaatccgcc gaaataagcc
    18841 gtctttccct tgaattgttc aagagaaagt aatgcaactg ctcctgcttg agccattgct
    18901 tcaacaatca atacccctgg cataacaggt tcttctggaa aatgtccttg aaaaaagggc
    18961 tcattgatcg ttacattttt cttagcaacg atccgttctc cttcaaccat ttcttccact
    19021 cgatcaatca aaagcattgg ataacgatgt ggaatgattt cttttatttc ttgtatgttc
    19081 attgttttcc ctctttcctg ctttgcttat ttttgctgaa tcctaaagag tggctgtcca
    19141 tattcgacaa ctgactcgtt ttctacaagg atttctgtga tcactccgtc ggcctcacta
    19201 gttatctcat tcatcagttt catcgcttca ataatgcaga taacttctcc tttggcaact
    19261 tgatcaccta cttttttgaa agctggtttg tcgggcgctg gttgcagata aacgattccc
    19321 acgattggcg aaacaacctc gattccatca aatactgccg attctttttc tttcattatt
    19381 tctggagcag ttgttgcttg ttcaggtatg acattgactg gctccgaaac tggcaacgtt
    19441 tcgtttgttt gaaccatatt catgcctcga cttgttttat ttttattcat atacaattca
    19501 aattgacctt cacgcaaatc gaattccgtt agcgaggatt gatcaaattg actgactaat
    19561 tctctgattt cattgatatc caaaattatt cctcccagcg tttcatgcac agaaccgcat
    19621 tatgtccgcc aaaccctaat gaattactca ttgcataagt aaaacttgca ggtttgcttt
    19681 cgtttttgac aactgtcaag tcaatctcag gatcttgttt ttctacattg atcgtaggtg
    19741 ggataaattg atgttctaat gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc
    19801 ccaataaatg tccagtcatc gatttcgtgc tgctgacgta agtatctctg aaattttcac
    19861 ctaatgcgat ttggattgct tttgcttctg cctggtcatt ggctggtgta cttgttccgt
    19921 gagcattgat atatccgact tgttccggct gtacaccagc ttcttccatc gcaagcttca
    19981 ttgctttaga tgcaccagaa ccatctggag taggagctgt catatggtaa gcgtcacagt
    20041 ttgcaccgta tccaacgatt tctccataga ttttggcacc gcgagcttgt gcatgttcta
    20101 aagattcaag aactagaacg cctgctcctt cacccatcac aaaaccattc cgattttcgt
    20161 caaacggaat cgaaccacga ttaggatcag ttgattctga gagtgctgtt aaagctgcaa
    20221 atccagcaat ccccatctca caaatcgttg cttctgttcc tccagcaaga atgacatcag
    20281 aatagccatg tttgatgtta cggaatgctt ccccaattga attcgttccg ctagcacaag
    20341 ctgttacgat agctgtacaa ataccttttg cacctacacg caaagcaata tttccagctg
    20401 ccatgttggc aatcgccatt ggtacgaaca ttggagaaac tcgttttgca cctttttcat
    20461 gtagacgaat aacttggttt tcgattgttg gaagaccacc gatcccagat ccgatgatga
    20521 cgccaaaccg gtcttgattc attttttctt tatctaaacc gctcatctcc attgcttcta
    20581 aagcagcgta gattccatag atcgaaaaca tatccattcg tttgctatct tttttaataa
    20641 aatatttatc gtaagggaaa tctttgactt cacccgctaa ggtaatccct gtttctgaag
    20701 catcaaattt agtgatcggt gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc
    20761 tgtctgcgtc atttccaata ggtgaagtga ctccatagcc agtaattacc actcgattca
    20821 tttgtttgct ctccttttct tatccgtgca tgaccaatcc gccatcgaca ttgatgactt
    20881 gtccggtgat atatggattt tgtgctaaga aaactgctgt ttgtgcaata tcttctactt
    20941 gtccgaaacg ctgcatcgga atttgctttt ccgcttgttc cttgactttg tctgctaaaa
    21001 cttccgtcat atcagttgtg atgaatcctg gcgcaatggc attacatgta atgcctctag
    21061 ttgcagcttc tcgtgccaca gattttgtta atcctactac gccagcttta ctagctgcat
    21121 aatttgcttg accaatattt ccaatcaacc cagatacact agagagatta atgatggccc
    21181 cttctcgctg cttcatcatt tttttcaata cgtgctgcgt catattgaag gttccaatca
    21241 aattgatatc cagacatttt ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat
    21301 ttgtgattcc ggcattgttt actaacacat ggatcgatcc aagcttttct tctgcttctt
    21361 tgatcatttg tcccgctttc tcataatcag agatgtcacc ggaaactcct acgcatttta
    21421 caccgaatgc ttcgatttct tctatctttt cttttggaat ctctccgcga ccattcaaaa
    21481 tgatatttgc tccagctttt gcaaaagcta aggccatcgc tttgccgatt cctcttgtac
    21541 tacctgtgat aaatacattt tttcctttta catccataac gttcctcctt atagtattgc
    21601 tattgtttca tcaaatgtct gcttattctc aacacgcaga actggtattg ttttatcgat
    21661 ttttttcata aatccgctta atacttttcc ggggccgact tcgatgactt gttcgatgcc
    21721 gattgttttt aacttatgga tactctcata gaaacgaaca ggttgcatga cttgctgttc
    21781 aagtagtccc gcgattgttt ctttcttcat tatttccgtt gttgtgttgc ttatgatagg
    21841 aaaagaaggt tcagagaatt gaatctgact taggtccttc gccagttttt ttgctgccgg
    21901 ttctaaaata gctgtatgaa aaggcccgct gacatttaga gggatcattc ttttgaaccc
    21961 tttttctttt aatagtaaaa cagcttcgtc tactgctttt tcttcgccac caatcacgat
    22021 ctgctgaggt gtattataat ttgcaggaga tacgatgccg tacttgctag cttcatgaca
    22081 gctttcctcg atcgtttcaa tcggcgcatt catcactgca accattttcc cgcttccagc
    22141 tggggcagct tctgtcatat aagcgcctct ttttgctact aacgcaactg cttcagtgaa
    22201 ggatagcgca ttactagcta ctaatgccga atattctcct aaactcagtc ctaaagctgc
    22261 atctgggatg attccatgct ccttcaataa gcgataatac gcgatgctga ctgttagaat
    22321 agctggctgt gtatattgcg tctgatccaa acgttcattt tctgtaaagc aaagttcagc
    22381 catatcgtag ccgagaatct cacttgcttc atcaaatgtc tgctttacaa cggcttcttc
    22441 atataaatct tttcccatac cttgatattg tgcaccttgg ccactaaata agaatgctgt
    22501 tttcatgttt ttctccaata tatccagaat atatgtttga ttcttcctat tcaaaaaggc
    22561 cgagacattc ctatttcagg aaatgtccca gcccccaacc taattttaat gattatgcag
    22621 tttgtttttc tacgtaagtc actaaatctt gaaccgtttt gattccgtct tctgtttcga
    22681 ttttcacgtc aaattcatct tcaatttcat tgatgatttg gaataaatcc aagctgtcag
    22741 cttctaagtc ttcttggatg tcagtagtca atttgatttc ttcagtttct tttcctaatt
    22801 cttctgcgat gatttcttga attttttcga ataccataat aatatattcc tccgatattg
    22861 tttgcttttt atttttataa tttaacagtc tacaactcca tgagaatact tccccatgtt
    22921 aaacctccgc catagcctgt aaatattact ttctgctgtt ttcctaaggt gatctttcca
    22981 ctagaaactg cttcatccaa taagataggg atgcttgcag cagatgtatt cccatattta
    23041 tccatattgg tcaggaactt ttcttgcggc atctttactt ttcgggcaat tttctcaata
    23101 attctgaggt ttgcctgatg caaaagcaga taatcaactg gtttgtcttt catgacttct
    23161 tggatattgt ttgcaacgtc tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc
    23221 aaataatacg aaccttctga atcttctgaa tagaacggat ttttattttg ctggtagcca
    23281 gcggtcaatg agctacttct ggtaccgtca gctgctaatt tttctttcag caattttttc
    23341 tgtggattcg cttctaagag aatcccacct gctccatcgc caaacaacac agcagtcgta
    23401 cgatcagacc aatcgattag cttagatagt gtttcaccac caatcaccag tcccttagtt
    23461 gagccgcagc ggatcatttt ttcagccatg cttaacgcat aaacaaatcc tgagcacgca
    23521 gcactgatat caaaagcgat agcatgagtt gctcccaatt tcccttgtac ttgtgctgct
    23581 acagatggtg tcgtgtaatc gggcgacatt gttgctacta aaataaaatc caaatcttta
    23641 gggtttagat ttttctgttt tatcaatttt tccgccacta aaatgcataa atcagaagtc
    23701 tcctgatttg ttacacatct tcgttcacga atacccgtcc ttgacgaaat ccattcatca
    23761 gaggtatcca tcatcttgga aagatcatta ttagtaataa tcttttcagg aaggacgctg
    23821 gctgttgcag taatcattcc aaattgttcc attaaatatc ctcacttata gtcttgcaag
    23881 aagtcgtgca agtttttcag tgcatccagc aaggcatgct gttcctccgt agccattccg
    23941 tctaagatgt tcttgaccat ttcacgatga aaatgctgat ggacccgaaa tagtaacttt
    24001 ccttttttcg ttagtcccaa ctttacaacg cggcggtcat cttcactgcg gatacgttcg
    24061 acatagcctt tttttactaa cttgttaata gcagtggtca aggtcccaac agtaattgat
    24121 agttctttgg ctacttctga agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg
    24181 tgcatctctg tgattgacag atctttgaat tgagattttt tcagctcaga ctcttcgatt
    24241 gtcaaaatat cattgaagac actaacaaga taatcattta ctgtttttaa atctggttcc
    24301 atgttccacc gtccactaat tagtttgatt atcaaatctt ttgatgttca aactatatct
    24361 taactttttt caaaatgcaa ccttaaaaga tcattttttt actatatgag ctttttttaa
    24421 gattttactc atttaaaaca actgataaat cagcatttat aatttctttt attagaaaaa
    24481 tcatctatct cacatatttt gattatcaaa gtattttctg tgaacagaac tatagaattc
    24541 cctaaaactt gtccattatt tcaaaatttg ttatagtctt tttattcaaa gcgtaaaagg
    24601 agatcctatg ttaaaagaaa aaatgaaaac tctccccatg ctgccgggtg tctatcttat
    24661 gaaagaccga gatggtaagg tcatctatgt tggtaaagca aaacggctca aaaatagagt
    24721 aagcagctat tttcatcaga ataaacagca ttcaaaaaaa gtattacgta tgattcatca
    24781 tataacagat tttgatttcg tcgtagtaga cacggaatta gatgctttat tgctcgagtg
    24841 tcaattgatc caacattacc gtccttttta taaccgacaa atgaattatt tcagtaatta
    24901 taattatgta catattacca ataagggatt tgtattaaca gatactccta cggctcgaac
    24961 atacgggcct tttcgtctct ataaaaaaat gccgtctatt ttgcgtataa tggaagaaac
    25021 ttatcaaatg ccttggttgt ctgaaattag tttgttagct ttgcgagtcc agcttcctga
    25081 cctacaggaa atgacttttg aacagaaaaa aaaggaactc caagggctgt ttcaaggccg
    25141 aaataaaaag ctactgactt acttgaaaaa aagacaacaa catttcatct atcagttgaa
    25201 tttcgaaaaa gccggtatgc ttcaaaaaga tatcgaatta gttacttatt ttattagacg
    25261 gatccaggaa caaaagcagt ttttaagaac accatcgctc accttcagca tgccattagc
    25321 agcagatgag agtcagaaaa agcactacct cgtttgttat ggtcaacttg cagaaacaat
    25381 gattacctct ggtgacgttt ctcctgactt ctattatgaa aagaaagaag ctcatttatc
    25441 tttgaaacgt caattgtcaa aagaagaaat cgatcctgta caaatcttga tcagttaccg
    25501 caaaaaatta gaaaaagaac agtttgaaat tgaactgtta aacaaaaaag aagctgaaaa
    25561 gcaactaaat tagttgcttc tcggcttctt ttaggcatct tcaacttcaa atgtcaatta
    25621 tcctaaaatt tattggatta agtcatagat ttccatcgct acgatatcga tattatcaaa
    25681 ttcgtattgc tgatgagtat ctgtttcagt taattcaaat gtatcggtcg ctttatcata
    25741 agttactgta cacttctcta cgccttcttt ttcaaaacgg cgaacttgca gttcattatc
    25801 tgctgactcg accatagcct ccagtcgttt tatgattgcg actaattgtg aatgtgtcat
    25861 cggaggtcct ccttttctta tttctgttat attgtatcaa aagcatagcc tacttgcatt
    25921 gattttctct aaaaaaactc gttttttatg attttttttc tggtattttt ccttcaggcc
    25981 accaaagttt gatcaaagct tgggttccgt cattctcaaa acctttatct gccaactctt
    26041 catatagttt caatgcttgg gtagttgctg gcaatacaag atccattttt tctgcttctt
    26101 ctaatgcaat ctttaaatct ttgataaaat gtttgacaaa aaaaccaggt gtgtaatctt
    26161 cttttaaaat acgaggagaa taattgctta aagaccagtt agctgcactt ccacctgaaa
    26221 gcgtctcaat cactttcttt aaatcaagtc cgtttttttg tgcatagaca agcatttccg
    26281 tcattcctgt catcgtacct gcaatcatga tctgattagc catttttgta tgctgacctt
    26341 tcccagcaga accatgcaat gtaaatgtcg taccaaattc tttgaataaa ggcagtactt
    26401 gatcataaac ggcttcttct ccaccgacca tgatcgtcag tgtaccattc tttgctccta
    26461 agtcaccacc ggataccggt gcatctaacg catctgcgcc tttttctttt gctgtttttg
    26521 caattttttc tgccaggctt ggtgtacttg tagtcaaatc aactaaaata tgtcctgaga
    26581 tatctgcaga aaaaatcccc gactgtccat agtagatttc ttctacatct ttggggtaac
    26641 ctaccattgt aaagagaata ttactttgtt ctgctaccgc ttttggtgta tctgcccaga
    26701 tagctccttc tgttactaaa ggatcagctt ttgcttttgt ccgattatag acagctactt
    26761 cgtgtcctgc ttctagtaga tggcgtgcga cagcacttcc catgacgcct gttccgataa
    26821 aaccaagttt cattttttcg acctcctaaa aatcaaaaat ccttgcttct atgctagtat
    26881 acgctagaaa caaggatttt ttcataaaaa atgtttgaat tttatctttt aaatgaattt
    26941 aattttattt tgattcgttc aatcgagtct tcttcttctt ttcttcgcat ctcttcttca
    27001 tgataaacaa ccatgtcttg ataaaattcg atcatagctt cgccgaaatg tgtagatgaa
    27061 atatcataaa gtttagcttc taaaactttt ggatctttag gaacttcact ggaaatataa
    27121 tcaataagtg ctggtgcaaa gtcttcgtcg gtttcaaaag ttttccctaa tgaaggatga
    27181 tcgaacaatc ggttcaaata agcatttcct tccacgacac aaggtgttcc ggctgccatc
    27241 gcttctgtat aagtaagccc ttgtgtttct gaagtagaag cactgacaaa ataatctgcc
    27301 gctttataat aaagtgctac atcattgttg tccacttctc ctgtgaactg cacaaattct
    27361 tctaactgca gttctgagat caattcctct aactcttcta cataaggtcc ttttcctacg
    27421 ataactaatc tggcgttagg ataagcggag atgacctgag gaaacccatt aatcaaggct
    27481 tggatatttt tctcataaga aatcctgctt aaagaaagaa tcatcaaatg gttttcctga
    27541 agacctagtg actcccttaa tttgctgata tcctgctgag tgatatcaga acgtttgaat
    27601 ttttcaatgt cgataccagt aggaatcacg cgaagcggtg ctattacacc atagctgcgt
    27661 aatgtatcaa taacacgttc acttggacaa actacacctg tcgtatgatt tgcaaataat
    27721 ctagacaagt attttacatg ggattgcctg atcacttttc ctttaccgat ataatgaagg
    27781 tagtcttcat acatcgtatg gtaagtatgg atcagaggta ttttcaactt cttagctacc
    27841 atcttcccaa gtatccctgc tccgaattct gtatgggtat gaataagatc tagttctagt
    27901 tctttggcaa ccaaataagc ataccacatg cctctaacaa caactcttcg atccttgaaa
    27961 gaaataaatg gtacacttgg cattcgaatc acatcttttt ccaacttatc cgcattagga
    28021 tcggtcgttg taaaaatata gacttcatgc ccatgtcttt ctaattcttg ttttaatgtt
    28081 ttgatcgatg tagcaactcc gctgacttgc ggaaagtagg tatcggtaaa aaagccgatt
    28141 ttcatgtttt tcgcctccaa actagtcctt gacctgattt acttttttta atttgctaga
    28201 gactttttca ttcagtacac tttcgtatac atttttaagt tcttcaccga ttttattgat
    28261 acttcgctct tttgctgttt gataaccggc ttgtgtcgta ctcggtagtt cttgattggt
    28321 aatctgttgg atcagtcgga taaattcttc gtttgtcttc cccatatagc agttcttgct
    28381 atcttctaac cagccattat acactggaat atctcgaaca agtacgtttt gtttgcttgc
    28441 taaagcttct aaaacaacga tcccttctgt ctcttcataa gaagggaaaa agaatagatc
    28501 tgcacctgaa tacgcacctt ctatgatctc tccacgaatg taaccaggaa acaggacatt
    28561 ttcaggatga tcttctttta caattttccg gatgttccga ggaatcgagt acatgggaac
    28621 atgaccaaac caaataaatg tgtattcggg catcttctta gcaatttcta cgaaatcaat
    28681 gattccttta cgttcgaaaa acaaaccgac acaaatgatt actttttgat ccggagacag
    28741 tttgaaatat tctctgaact tttgttcttt cattggatca gggtgatatt tctctaagtc
    28801 gatcccattg gaaatagctt gaatcggcac acggatacca tagctttcca acaactgttt
    28861 tgaataaggt gttggcgtga tcagatagtc cgctttttgg tacaatccta ccagatattt
    28921 ttttacgata ggagaaagct ggttcgatct gataaaggaa tttcggaaat cttcctctgt
    28981 agaatgcgca tgatagacaa ctttttttcc ttctcttctg gctttattga ccatattatg
    29041 actattgatt ccgtatgtat tgatgtgcag gatgtcataa tcttcttttt catctagtgt
    29101 ataagagata ccgacttccg tcagagcacg tttttgatga tcaagcgcac gcccgattcc
    29161 agattttgca agcatctttt cactttcaaa atataataat accttcacct tacgtccccc
    29221 ttcacttata tagtataaca tagcctacaa actgtcactc aaacttaagc cctatttttg
    29281 tctattctta ctgacaaaaa cgttttctta aaaaagaaca aaaaaactgg cgtagctctt
    29341 ttacaagcca caccagcttt tgatacgttg tttattttac atattcatga acaagttcaa
    29401 cgacttcttc catcatatcg cattctttca atgcacgatc agcaagttca gccattttcg
    29461 ctgtgtccaa gcgtttcatt aaactacgcg ttttaagaat agatgtcgca ctcattgaga
    29521 actcatctag acccatacct actagcaatg gaacagctgt ttgatctcca gccatttctc
    29581 cacacatacc tgcccatttt ccttcagcgt gtgctgcatc aattacattt ttgatcaaac
    29641 gtaaaattga tgggttatat ggttggtaca agtaagaaac gcgttcgttc atacggtctg
    29701 ctgccattgt gtattgaatc aaatcgttcg tacctacaga gaagaaatct acttcttttg
    29761 caaacttgtc tgctaagact gctgctgctg gaatctcgat catgatgccg acttggatcg
    29821 tatcagatac ttccttgcct tcacttatca atttttgttt ttcttcttca aagatcgctt
    29881 ttgctgcgcg gaattctttg agtgtagcta ccatagggaa catgatacgt aaattaccat
    29941 gaacagatgc acgaagcaat gcacgcattt gtgtacggaa catttcgtcg ccttgttcag
    30001 ataaactgat acgcaatgca cgatatccta agaatggatt catttcatga ggtaattgca
    30061 gatatggtag ttctttatct ccaccgatgt ccattgtacg aacaactaca ggttttcctt
    30121 ccataccttc taatacagct ttgtaagctt catattggtc atcttctgtt gggaagtctg
    30181 gagaatccat atacaggaat tcagtacggt acagaccaac tgcttctcca ccattattat
    30241 ggacaccaac tagatctttt ggtgttccga tgtttgcagc taattcaaaa tgcttgccgt
    30301 cagctgtgac agtttctgca ttttttaatt tttcccattc tgctttttgt gctgcgtaat
    30361 ctgcacctgc tttttcaaat tcagcttttt gttcatctgt aggatcgatg attacatcgc
    30421 cttcgatccc gttgacagcc aaaatatcgc cttctttgac tttagcagta atttcttttg
    30481 tcccaacgat tgctggaatt tctaaagaac gtgccatgat agcagaatgt gaagtacgtc
    30541 caccaatgtc tgttacaaac gcttttacaa aattgcgatc taattgtgct gtatcacttg
    30601 gagtcaagtc atgtgcaaca acgaccactt cttcgttgat cattgatgga tttggtaaag
    30661 taacgcctaa taagtgcgct aaaatacgtt tagcaacgtc acgaatatct gcagcacgct
    30721 cttgcatata agcattatct tccattgctt cgaacatacc gatatacata tccgttactt
    30781 cttttagcgc tgattcagca tttacgctgt tgtctttaat attttgcttg atttgaccta
    30841 ccatttctgg atctgaaaga accatcaaat gggcatcaaa tacttgcgct tctgcttcac
    30901 ctaagctttg cgctgctttt tcacgaattt gctgcaattc ttcagtagat ttagctaatg
    30961 cagcatccaa acgagcttct tcagccgcag tatcttctac tgttgcctta ctgaatgtta
    31021 aatccggttg aactagcagg taagcttttg caacggctac accgtcacta gcggcaatcc
    31081 cttttagcat ttcaaccatt attcagataa gccttctttc ttcattgttt caacgattgc
    31141 tgccattgca tcagcttcgt cagcaccttc tgcagtaata gtaacatcag aaccttgacc
    31201 tacacctaaa gacataacgc ccatgattga tttcaagttt actgatttac ctttatactc
    31261 taagtttaca tcagagttaa atttgcttgc tgtttgtact aatagtgtag ctggacgagc
    31321 gtggatccca gtttctgcta ctacgtgaaa ttctttcttt tccatattaa tcggtctcct
    31381 ttaagtgagt gttgttttta tgtgagggtt ttctttttta gtaattcaga gaatcctaac
    31441 aaatgccacc ctttcatctg taaagattaa catttttttt cgttagatac aacagttttc
    31501 attcaatttc agataaaagt attcaaatta ttccggattc tttttcatcc ttttcttcac
    31561 attttataac aaacgtgttt atttcctttt tcttgtcgct ttctcttctc ctgacgttta
    31621 tcattctttc ctttgaccct ctttgttttc atatgaaacg aatta
//
LOCUS       DAYYHX010000009        29113 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000009 DAYYHX010000000
VERSION     DAYYHX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.009"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            274..594
                     /locus_tag="ACHA6Y_000305"
     CDS             274..594
                     /locus_tag="ACHA6Y_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8986428.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            748..1653
                     /locus_tag="ACHA6Y_000306"
     CDS             748..1653
                     /locus_tag="ACHA6Y_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8986429.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1650..2423
                     /locus_tag="ACHA6Y_000307"
     CDS             1650..2423
                     /locus_tag="ACHA6Y_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8986430.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2604..4028)
                     /locus_tag="ACHA6Y_000308"
     CDS             complement(2604..4028)
                     /locus_tag="ACHA6Y_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8986431.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4239..4685
                     /gene="nrdI"
                     /locus_tag="ACHA6Y_000309"
     CDS             4239..4685
                     /gene="nrdI"
                     /locus_tag="ACHA6Y_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8986432.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4750..6732)
                     /locus_tag="ACHA6Y_000310"
     CDS             complement(4750..6732)
                     /locus_tag="ACHA6Y_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8986433.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6746..7642)
                     /gene="murQ"
                     /locus_tag="ACHA6Y_000311"
     CDS             complement(6746..7642)
                     /gene="murQ"
                     /locus_tag="ACHA6Y_000311"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8986434.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7645..8706)
                     /locus_tag="ACHA6Y_000312"
     CDS             complement(7645..8706)
                     /locus_tag="ACHA6Y_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8986435.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8917..9444)
                     /locus_tag="ACHA6Y_000313"
     CDS             complement(8917..9444)
                     /locus_tag="ACHA6Y_000313"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8986436.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9511..11013)
                     /gene="eat(A)"
                     /locus_tag="ACHA6Y_000314"
     CDS             complement(9511..11013)
                     /gene="eat(A)"
                     /locus_tag="ACHA6Y_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8986437.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11271..12392)
                     /locus_tag="ACHA6Y_000315"
     CDS             complement(11271..12392)
                     /locus_tag="ACHA6Y_000315"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8986438.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12518..13459
                     /locus_tag="ACHA6Y_000316"
     CDS             12518..13459
                     /locus_tag="ACHA6Y_000316"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8986439.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13609..14505)
                     /locus_tag="ACHA6Y_000317"
     CDS             complement(13609..14505)
                     /locus_tag="ACHA6Y_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8986440.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14774..16114
                     /locus_tag="ACHA6Y_000318"
     CDS             14774..16114
                     /locus_tag="ACHA6Y_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8986441.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16332..16775
                     /locus_tag="ACHA6Y_000319"
     CDS             16332..16775
                     /locus_tag="ACHA6Y_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8986442.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17279..18697)
                     /gene="nhaC"
                     /locus_tag="ACHA6Y_000320"
     CDS             complement(17279..18697)
                     /gene="nhaC"
                     /locus_tag="ACHA6Y_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8986443.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19100..20323)
                     /locus_tag="ACHA6Y_000321"
     CDS             complement(19100..20323)
                     /locus_tag="ACHA6Y_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8986444.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20354..21193)
                     /locus_tag="ACHA6Y_000322"
     CDS             complement(20354..21193)
                     /locus_tag="ACHA6Y_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8986445.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21210..21890)
                     /locus_tag="ACHA6Y_000323"
     CDS             complement(21210..21890)
                     /locus_tag="ACHA6Y_000323"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8986446.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21892..22965)
                     /locus_tag="ACHA6Y_000324"
     CDS             complement(21892..22965)
                     /locus_tag="ACHA6Y_000324"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8986447.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23021..23259)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23302..23535)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23612..24283)
                     /locus_tag="ACHA6Y_000325"
     CDS             complement(23612..24283)
                     /locus_tag="ACHA6Y_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8986448.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24370..27018)
                     /gene="mgtA"
                     /locus_tag="ACHA6Y_000326"
     CDS             complement(24370..27018)
                     /gene="mgtA"
                     /locus_tag="ACHA6Y_000326"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM8986449.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27360..27534)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27707..28144
                     /gene="ndk"
                     /locus_tag="ACHA6Y_000327"
     CDS             27707..28144
                     /gene="ndk"
                     /locus_tag="ACHA6Y_000327"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM8986450.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28166..29032)
                     /locus_tag="ACHA6Y_000328"
     CDS             complement(28166..29032)
                     /locus_tag="ACHA6Y_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8986451.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
BASE COUNT         8417 a         6111 c         5162 g         9423 t
ORIGIN      
        1 tgtttttttt aaatgattaa taaataacaa cttctttcta tgaaattgca aagcattcat
       61 cttcttttcc gctaacgata tatccgtcag tgcttggtac actttactaa aaagtatgag
      121 attgccgata tctggatagg tgtgctatac tacagaaaaa gatatacaat ctgccaccgg
      181 gtaggataaa gaggatgatc attccattag gtctttgaag ggatgatttt cctctttttg
      241 tgtatttttt tatagttaag gaggaatgta agtatggcat ggatggaatt aatcatagca
      301 ggaattttgg aagtattctg gtcaacgatg atgaaatgga gtgatggttt ttctaaaatc
      361 aattattctt tttacacagt gattggcatg attgctagtt tttacttttt gtcaaaggca
      421 atcaaaagtt tgccgatgag tctctcttat cctatctgga cagggattgg cgcagttggt
      481 tcggtcataa ttggtgtggt cttgtttcat gataagctga atctttccac ttggttcttt
      541 gtggggatgc tactcatcag tatcattggc atcaagatta ctagcggcca ctagacactt
      601 ggaataagta ctgatcagaa aaaataagcc tttttattta atattcaatt tttttatgaa
      661 aaaataagaa ataagtccaa tgacctagcg acttctccac tcatcaggtg tatgcttgta
      721 ctatctatag aaaaggagtg aatatttttg aaaagtcttt cggtccaaga attatccaag
      781 aactttggtc acaaacaagc cctcagcgat gtcagtttta gcttatcccc tggcaaaatc
      841 gttggattag taggtcctaa tggcgctgga aaaacaacga tcatgaaagc tattttagga
      901 ctgatccatt actcagatgg aacaatctgt attggtgaaa atgagatttc tccctctagt
      961 cataaagggt tggaacaagt aggagcattg atcgaatacc cgggtatcta tccattctta
     1021 acaggctatg atcatctgaa gttattctct gaaaccaacg atgtatctgc tatcgacact
     1081 atcgtgaatc agctcaagat ggaaaagtac attcacaaaa aagcgaaatc ctactcgttg
     1141 ggaatgaaac aaaaattggg aattgctctt gccttactga accatccgtc tatcgttatt
     1201 ttagatgagc cgatgaacgg tttggatcca caagcaacaa gagatgtgcg aaaaatcatt
     1261 accgacttgg ctactcaggg cacctctttc ttgatctcca gtcacttact cagcgagctt
     1321 gaaaaaatgg tcgatgagct gatcctgatc aatcaaggaa agatcatcaa acaatgtaca
     1381 atggctgaat tgaaccaaac agcgcaagat ttcatcgtta tctctacaac aaatgatcta
     1441 gctgctcgaa cgatcttgac agaagctgga tatactttgg aagatcctga taaaatcaag
     1501 cttttaaaag aaaacgaaga attattagga accgtgatcc agctattgtc tgcacaaaat
     1561 atccatgtga cagacgtcca gcatctgacg aatgatttgg aatcttctct attggaaatt
     1621 ctggattcag taaaggggga agaagaaaaa tgacattgct tgtaaaacaa gaattattta
     1681 aattgatcaa gaaaaagtca actgctgttt tatctgttct attagttgtt ttgctcatag
     1741 gaacagcact tctagccaaa aaatacacga cgatcattga tccagttgaa atgactgctc
     1801 agctttttag tgccacatct tggattgtct ttatcatgat tgcagctgca agcacgatca
     1861 tttcgatgga agcacaatat ggtacattga aaaatctcct ttatcgaaag tattctcgag
     1921 gagaaatatt agtaagcaaa tggatcacat tagttattta ttctgtttat ttataccttt
     1981 tagctattat tgtcactgtg ttgatgaaac tgattctttt cccttcgatt tccttcacgg
     2041 agaaggtttc tactggtcaa acactgatcc aaagcttgtt cacttatact ttgggtagtt
     2101 atatcggttt atggctgatt ttaagtttag tgttgatgat tgcttgcttt atcaatagtt
     2161 ccggcgcatc catctcagca ggaatcgtct tttattttgc ttcgtcaatc atatctggta
     2221 tccttattgc actcatccaa aaatgggagt ggatcaaatg gaatccgatc agtatgttga
     2281 atctgcaaaa ccaaattgga aacgaagaga tcatgaagca attgactcat ctttctacta
     2341 atcaaatgct attcggtaat ttagcttaca tcgttttgtt cttagcatta ggatatctcg
     2401 tatttaagcg aaaaaatgta tgatgactca tcaaaaaaga ggctgtgaaa gagacgttca
     2461 gccttattgc cgattggctg tctcttaaac accgtttatt ctgaaaaagg agctgtgaca
     2521 acatttgtgt cacagctcct ttcctagtat gtttttgaat aaacgacagg ctcacaacct
     2581 ctctttatac ttcttttata cttctatacg tgaagcatcc cagtggcaaa taaaaccatt
     2641 gcaatcactc cgaccatcgt cacacctata agtaaaatct tatctaagtg gatatgttgt
     2701 acaggctcgt tttgctcctt acgtgctttg aggtaaaaat aaatgcctgg caagaaaaga
     2761 acaagaacaa gcattacttc attaaaggct acgactagca tagcccatac ttgaaaggca
     2821 cttgctaaaa gccctactaa aaattggacc cattctttcc gctgataaga aagtttcatt
     2881 tggtacaatc ctaagaaaag ccacgtgaag aaaatagctg ctgtaccaag tgtgtaagca
     2941 aagttataag cactgtcagt gaataataat gaaagtaaaa agacattcgt acataacgtc
     3001 gtcaatacga tagcataagt tggtgcccca tttttgttga gttttcccca tgctttaggc
     3061 aaaagtccgt cttcagccat gtttcccatt gtttcaatcg gtaagatcgt ccatgagagc
     3121 caaacaccaa ctaaagaaat gatcaaaccg ccgttgatca cgactgctcc ccatttacca
     3181 accatcattt caaacacata gcccatagca ggctgcgcct tgatcgtttg cagctgattc
     3241 atcgtcattg tcccatatgg aagcatggaa accaagacgt aaataataat caagccgaat
     3301 atccctagaa tcgttgcttt cccaacatct gattgttttt ttgctcgtga acctacgaca
     3361 gaggctcctt caataccaac aaatacccaa agcatcacca tgatgcaact caatacttga
     3421 ctggaaaacg ggatcggctg tccattcgca ttcaatataa tgtcgctaga aaagttatca
     3481 atgaattgtt ggatcgaaaa cgtgctgata gctactacaa taaataaaaa taatggcact
     3541 aatttacaga atgtgacgat agcattgacg aaactagcat tttcgactcc ccgattcact
     3601 agccatgcat atccccataa aaagactgta ctcatcacga tcgatgccag attttgccca
     3661 ccttcaaata ctgggaagaa atatcctaat gcgctcatca acaatgttgc aaaagctacg
     3721 ttgcctaacc agcatgtgag ccagtaaccc caaccgctga taaaaccagc gaaacggcca
     3781 aaaccttctt ctgcgtaact aaagatcccg ccttttaaat ctggacgttt tttattgata
     3841 ttattgatcg acaaggagag catcaacatt gctgttccta cgagtaccca actgaaaata
     3901 gctgggccgc cagccgatcc ggagatgtcc gtcataatac caaatacgcc acctccaatc
     3961 gctgaaccaa ttactaaggc agcaagtgga attaaaccaa ttcctttttt ctgttgctct
     4021 gtatccatgt aagcatcctc caaaataaaa ccgcttttat caaaaagtat aacaaacctt
     4081 ttttattttt acttaattaa taaaaacttt aatagaaata taattctacc ggcttttttc
     4141 tcattataca agcaaagcgc ctagtccctt tccaaattac accaccaaaa aacttaaaaa
     4201 agatgctata ctaaaaggaa agaattggag gatgatccat gaacatttta tatatatcga
     4261 tttcaggaaa tacgcgtgcc tttgccaaac atctagcaga atatgctgaa aaaatgcatg
     4321 cagaagatcc tgtcaatcct gaagttactt taaaagaaat ccatgaaaat agtgatttta
     4381 cgaaagaaac agaaccattt tttactttcg ttcctactta tttagacggc ggaaatggtt
     4441 tagataacgg tgatacagaa atcttgaccg aaacgatgcg tgaatatctg gaacatgaag
     4501 acaatcataa actttgtctt ggtgtagtcg gtagcggaaa taaaaacttc aataatcagt
     4561 attgtctaac agccaaacaa tatgcacaaa catttggctt tcctttcttg gcagattatg
     4621 aattacgggg aacaccatct gatgtgacac gaatttatca gattttagca gaaaacaata
     4681 aataataaaa aagaagaagc tgaaaaatag atttcagttt cttctttcgt ttctattgtt
     4741 tctatcatat taagctttga catccatcac aggtgctttt gcttttgctt tacctgtttt
     4801 ttccaagaca aagtttttta cacgatccat attggttacc aagacgatca tggatgtttc
     4861 tttgcctgct gctttgatcg cttctaagtc agcttctgct acaagcgtat caggtgtcac
     4921 ttgctgccct tctgttactt tcacatcaaa tggcgtaccg cctaagtcaa ctgtgttgat
     4981 tcccatatga agcaagattt ctaaaccatt tggcgttgtg atcccaatag cgtgtttcgt
     5041 tgggaagaca gagctgacta ccccagtaac tggagaatag atatgtccgt tttctggttc
     5101 tacaaagtag ccttcaccca tcatcttttg tgcaaaaact ggatcacttg ctttatctaa
     5161 agaaacaact gttccgtcag caactgatga gaggatagtc acactttcag ctgaagtttg
     5221 aggtactact ttttcaggca cttcatttac tgtggctgtt tcagtcgcta attcttcttt
     5281 ttctaaacgg tctttaggaa taccgaagaa gaatgtagca acgaagccac cagcataagc
     5341 tgccaataat ccaaggatgt agcctaacca tttaccgtca gagatcaatg ggatcaaagc
     5401 ggctcctgaa ggtccaatag cgatagcccc gacatttccg atcattccga tcactgcccc
     5461 gccgatacct ccaccgatac aagcagtaat aaatgggcgt cctagaggta atgttactcc
     5521 gtaaatcaat ggttcgccga ttcctaggat cccgactggt aaagctcctt tgatcatttc
     5581 tgttaattct ttatcttttt tgcagcgaat ccataatgcc aatgctgcac ccacttgtcc
     5641 tgcaccagcc atcgcaagaa ttggaagcaa tagcgtacga ccagtttcat caatcatttg
     5701 cacatggatt ggagtcaaaa tttgatgcag tccgaacata accatcggca agaataatgt
     5761 gcctagaacg aagccggcaa atgctccacc aacgccaagt acccagttga tcccaccgac
     5821 catgccatca gaaatgaaac ctgctaaagg catgatgaag aaaatttcaa tcagaccgat
     5881 ggcaatcaat gaaagagttg gtgtaacgat gatatctacc gcatcaggta ctactttatg
     5941 cagttttttc tcaacgattg acaatagcca aacagcgaag atgacaccta ggatcccacc
     6001 ttgtccagct gcaagagctt caccagtaaa taagtttttc agtggtgctt ccggattcat
     6061 ccctgtcagc atcacaacgg ccccgataac tccaccaaga gttggtgttg caccaaaaac
     6121 ttgtgcagca ttgatacctg tatagataac taggtagcta aacatcccgt ttttcaagat
     6181 gttcatcaca tcgatatatt gttgccatgt tgctgcatca agtgttccgg ctgtgactaa
     6241 gtttgctaaa actgcggcaa tccctgccac gatcccagta ccgacaaatg caggaatcat
     6301 tggcacaaag atattagaaa tgtcttttaa taccgatttg attttagatg gtttttgttt
     6361 cgatttttgt gccgctttca tttcttgtgc ccgttcgttt acacggtctt ttccagatgc
     6421 tgatgaattt gctgtagctg tacttacaga agaaagtttt tctcctaatt tcacgcctgc
     6481 caaatcaacc atttgctgtg ctactttgtt cactttccca ggtccgatga tgacttgaag
     6541 ctgttcatct tctactacac caagcactcc tggaatcgct tttaattcag agacatttac
     6601 aagatcatca ttgatgatct tcatacgcac acgagtcatg cagtggacaa tgctttctac
     6661 attgcccatg ccgcctgccc ctttataaat ttgttttgcc agttcttcca aagtcagttc
     6721 gttttttgcc atggtctttt ccccctcata aggtcccttt tacaaatccg tttgcttcag
     6781 ctaattttgc ttgagcttcc tctttatcac tatttgtcaa aatcatgacg atagccagct
     6841 taacattttg atctgcttct tcaaagtact tttcagctgt ttcgtaatca gcttctgtcg
     6901 cttgcatgat gattcgtttt gagcgttcga ttagtttttc atttgttggt ttgacatcga
     6961 ccatcaagtt attgaatact ttacctatac caatcatact gatagtagaa atcatattca
     7021 agataagttt ttgtgctgtt cctgacttca atcgagtaga gcctgtcaaa aattctggac
     7081 ctgcatcgac ttcgattggg aaatccgcgt gtttactgat ttcggcttgt ttattgcaag
     7141 aaatcgttcc tgtgacagca ccgatcgatt ttgcgtaatc taatccaccg atcacatatg
     7201 gtgtacgacc gctagctgcg ataccaataa ctatatcatt ttttgtcaaa ttcagcttga
     7261 ttagatcttc ttgccctagc tcataagaat cttcggcccc ttcgacagct acagtcattg
     7321 cttcttctcc accagcaatt agacctatga ccatttccgg ttctacaccg aatgtcggta
     7381 cgcattcagc agcatctaac acacctagtc ttccacttgt tccggctccc atataaatca
     7441 gacgtccacc ttttttaaag gactcaatcg tgttagcaat cactggttcg atacttgtca
     7501 gttctttttc aaccgcagcg gccactttct gatcttcttt gttcattagt tgaacggctt
     7561 gagaaatact catctcatct aatccaaaag tttcttggtt tctgcgttcc gttgttaggt
     7621 tctctaaatt gattttgctc atttttatct tccttctttt cttataaatt gatagttcat
     7681 accagctttg atttggttga tcaatggtag ctcttctttg ataactttag ctactacatt
     7741 cactttttca tccgctggca aatcatattt ggttaattgg atctcaccca tatagcgcaa
     7801 atacttttcg ttatctaaag taatagaccc tttcatacgc gtagctgtat ttcttgctgg
     7861 gataggcgga atctctcgaa aacgtgcatc tacgcttcgt atgacatctc ttgcatcgtc
     7921 ttgccgattc gtatgcttcc ctaatatata ttcgtaaaat tcttcatcta caggttccgt
     7981 atggagcaag actgtctgtt ctttttggaa agaagcaaat tgctcctgaa cgttttctga
     8041 cagacctgca tctccgatgt aaacaatatc cgtgttactt gctaatagat ctagagcgcc
     8101 tgcaagagga tggatccctc gatgttcttc caaagttggc aatccttgat atagtgggcc
     8161 tctcaactct gtatctcctg gtacaaaacc ttggacaagg agaccttgaa ttttgagcca
     8221 ttgatttttt ttctgatacc attctttgtc cagccctgtt tcgggacgtg gataataatt
     8281 atgccaagct tcaatctgtg agaaatcagc ttctgcttct ttcaattcat cgatatcttt
     8341 aggcgtgatc gtgctagcat tcaaactgat tttcaattga tgtgaccagt cggcaatctg
     8401 ctgattggaa atatgataat ccattcttaa acctgttaca ccaattgctt tcaaaggcgt
     8461 tagatcttct aatgaaaaac ccgctcgttc caatgcctct cctgaaatat cgaccatcaa
     8521 ttctagttta ttctttttcg cttcgcttcc tagttcgatc aatcgctttt gatatgccga
     8581 aacatcatct tctggtatat gcatggaagt gaaaataccg acaaagccat tggatgccat
     8641 ttggcggata tactgtttct tctcttctga caaagaatcg ttcataaata ttgaaaagcc
     8701 aaacatatta tccctctttt tctgtatccg tcttcatttc tatgactaga gtatatatct
     8761 aaagaaacaa aatttcaata cttttattaa aataattaaa tttcatttct aaaggttgag
     8821 tttatattat caaaagaatt gtcataacct aaaaaaggca ctgctaagca aaagcagtgc
     8881 cttgaaatat tttatttaaa taacacaaaa acataattac tcataagaaa ccaattctgt
     8941 attatctacc atgaatgtaa agaacccctc attaggcatc ggatgaaaat aagcattgat
     9001 cactcttatc aaggcattat ggcaaactaa cagatagaca ttttcttcgt cttcgatgca
     9061 ttctttcaat aaaggaacga tccgtgcata cacatctaag actgactccc catttgggaa
     9121 tcgtacagcg aacgctaaac gtgctttttg aaagttctca tctttgcttg gcagtccatc
     9181 ataatcccca aaatccattt ctaccaatcg ttcatcaacg gtcagcggca agctgagacg
     9241 atcagcaact gcttgtgctg tatctcttgc tcgttgaaga ggggaatgga tgattttcgt
     9301 gattggaaca ggcaaatcta ctaccttttc tgctagtttc tgtgcctgca tctgtccttt
     9361 ttcagtcagc ggcacatctg cccgtccgca aactaatcct tgtgcgttcc aagttgtttc
     9421 tccgtgtctc gttacataaa gcatgtgatt ctctccttcg ttggcaaagt tccccttaac
     9481 cataccttgt tgttcttgag attacaaggg ttagattctc tcaagagaaa taatctcagt
     9541 agatacttta tccaggaagg tttgatcatg ttccactagt aacatggcag gtttcacgtt
     9601 caagatcagt tgttctaatt gttcttgatt gaagacatcc aaataattca atggttcatc
     9661 ccatgtatat agttcagctg gctgtgataa agatttagcc aattccactt ttttccgttg
     9721 gcccatactc atctgctcga tcttgttatg aaaaacatct ctttccatcc caagctttcg
     9781 gaggttgttc aaaaatgctt ggtagtctac ttggtttttc tctgcaaatt ccgccaacgt
     9841 tcctcgattg tcttcataat tttggcttgc ataactaatg ctcagatgtt ttggctggta
     9901 tttttctcct ataaccttgc cgttgaatgc ccccagaaga taatggataa tggatgactt
     9961 tcctgcaccg ttaggacctg aaatcgccac ccgctgatga ggctcgattg taaaatgaat
    10021 tggctcgaat aacagatttt cataccctaa ttgaagatct tctacgctta aaagtcgctt
    10081 atggtgagac gcttggctat tcatcgtcaa agaatcgata tattcgatat cttttaatag
    10141 tttttctttc tcgctgatct gggtctccat ccgattcacg attgctttgg atcgtttcat
    10201 cgtccgtgca gcatcagcac ccactgctcc tttattcact cgtctagatt cagtatcgat
    10261 gaatccgact tgtttctttg ttttatctcc ttctcgggaa cgagaccatt cggctttttc
    10321 agctgctgtt ttctttagcc tactgacttc ttttttcaat ttttcatttt gagccatttc
    10381 aaattcatca cgaagttttt tctgttcttc atagatagag aaattccctt gataaagttc
    10441 cagttgactt ttttcgattg ctaaaacatg gtccactact tcatcgataa atccccggtc
    10501 atggctgacc acgatgaagc cttgtttttt ctttttcaaa taagccgcta cttgttttct
    10561 accagagata tccaaatgat tcgttggttc atcgattaac gggaaatgag tgtcatccac
    10621 aaataaaagt gccagtagga cttttgtctt ctctcccccc gatagtgtgc tgaattctct
    10681 ccataagatt tcaggatctg tttgcatcaa ttggagctct ctttcgattt cccatatctc
    10741 aaaatccgta atatcattta acacgtaata ggttaaacgt tctttatctt ttgtctgttg
    10801 cgggaaatag gcaaattctt gctgatggat tacttgccct tgataaggta gtttgttttg
    10861 tagaatattc agtaaagttg tctttcctcg accgtttcgt ccgataagtc ctagtttcca
    10921 ttgtgtgtca aaatttagat ttgcttgttc aaataataat gtgccttggc tgtcgtagcc
    10981 gaatgtcaga ttttttattt cgattttaga cattttccat cacctcgtaa aaaaagacca
    11041 gtcctatttt cagaactggc taaagaggat gcaagggttg gatacacaca aaaagaccca
    11101 tacatcgaaa aaaccaagcc aatcccgaaa atctgcacac tgcttccttg tctccacaag
    11161 gtacagtctg aatgcagctt tcatcaatcg acttaatttt tcaatgcacg gatccctcac
    11221 tttgtcctat atttgaaatt acgatagcat aaagaaaatg attttgtcaa ctacttcggg
    11281 aggatggtga aaaatctgct aagtgtctgt cctaagattt ttgttttttc taatgtttgc
    11341 ctgtgttcca ttgcttcttc taaacctatt ggacccaaat gaatacttaa gacaattggg
    11401 agaaaatcag aaagtccttc tagctcttgg tctctacttc cgcaaatagc gattattggt
    11461 tttcgatatt tttttgcaag cactgctaca ccgtatggaa cttttccgtg gatcgtttgc
    11521 tgatcgatcc gcccttctcc tgtaatgatg acatctgctt gttcgatctt tttttccaat
    11581 ccagtgattt ggctgactaa atcgaaacca gaaaccatct tcgctcccaa caaaaaaagg
    11641 gctccaccaa taccgcctgc tgctccactt cctggaatat catctaaatc cacctcccag
    11701 aaatctttta tttgctgagc aatctttcct gcttgtttat ctaagaaagc tagcgtcgta
    11761 tctattcctc ctttttgtct tccaaattgc caagcagctc cttgttgacc tgtatatgga
    11821 ttggtaacat ctgcagcgat aatcaatttt actttagaaa caagaacgtt tcttcgatca
    11881 aaatgaagtt ttttagtaga taataaagga tttccttctg tgtacccttc taaagaagca
    11941 cccaaggatt ggagtaatcc tagaccacca tcgctacacc cgcttcctcc taaagtgaca
    12001 ataatttgtt ctacttcttg aagcagtgca tctttaataa gttctcctaa ggcataactg
    12061 gaagcaagtc ggatagtttg atccgttggt ttatcaagta gatctaaccc caatgcttgt
    12121 gcagattcga tgactgcggt gttttttcct tgccaagttg tcaataaata gctgattttt
    12181 cgctgacgga ataagagatc catcccatca atggtacgcc atgttcccct gagcgcgtaa
    12241 tagattgctt ctgcagtatt ttctccgcca tctgcaatcg gcacattgat ttttcttcct
    12301 ttacgatctt cccagccatt tagaactgcc tgatttagtt caaatgaaga agcacttcct
    12361 ttaaatgaat caattgccgc caaaatatcc atctgtatcc ctcactttct tcttaagtaa
    12421 gacacttttt ttcatttttt tcaaacaaaa caatgctatt ttcttccatt tttctatata
    12481 ctaaaaggga actcattgaa taaaggagag aaaaaacatg aaaatcgcaa tagctggggc
    12541 cggagcaatg ggcagccgaa tcggattgat gctacatcaa agcggaaatg aggtcctctt
    12601 gatcgatcgt tggcctgctc acattgaagc cattcgtaca aatgggttga ttgctgactt
    12661 taacggtaaa gaagtggtcg caaaactgcc aatctattca ccagaagaaa tcatcgaaag
    12721 caacgaacac gtggacttga tcgtagcatt aacaaaagcc aatcaattag atgatatgtt
    12781 ttgttcgatc caatccatta ttactgacaa tacttatgtt ttgtgtttac taaatggatt
    12841 aggtcatgaa gatgtactgg aaaaatatgt gccaaagaaa aatatcttat ttgggatcac
    12901 gatgtggaca gctggtctag ctggtcccgg aaaagtcaca ttgttaggtg atggagaaat
    12961 cgaactagag aacttggaac cagaaggcga agcatttaca aaaaaagttg tagaagtctt
    13021 tcaagaagca aatttaaatc cgatctatag ccataacgtt cgttattcca tttggcgaaa
    13081 agcttgtgta aatggaacat tgaatggttt atgcacgatt ttggattgta atattgctga
    13141 actaggcgcg caaaaagcgg cagagagcat ggttcgaaca atcgtatctg aatttgcttc
    13201 tatcgcagca aaagaaggaa tcatactaga tcaagaagaa gtgtaccaac atattgcttc
    13261 tacttatgat cctgacaaca tcgggttgca ttacccatct atgtaccaag acctgatcaa
    13321 aaaccatcgt ttgactgaaa tcgactacat caatggtgcc atttggcgaa aaggacaaaa
    13381 atatgatatt gctacaccat attgtgcctt tttgacccaa ttagtccatg caaaagaagg
    13441 aatccttggc gcagaataaa aggctctctg atgagaatca gctgaatcag cagctggttc
    13501 tcttttttta agcccaataa aagatcaaaa agaaaacaca actctcatga tgccataaga
    13561 attgtgcttg tttgtttcag tacataatct cttcaaaatg taggtttttt atttgttttc
    13621 aacagaggag aggtcatatt cgtattgctc ataaatagtg gaatcgttag tgataaatcg
    13681 ttttgcccaa ttttctgcat cataaaattc caatagctct gataggattg gcttaaatgg
    13741 tgtatgcaga aattccggta attcatctaa atcacgttct cgaagcaaaa aaatattttc
    13801 aggacgataa aaatgataat gagcaatcgc ttgattatcc gtcactagtt tcttttgaaa
    13861 atacatggat tcaaatgtcc gcaaagtcac tccttgctga ccctcccgca acagttccag
    13921 aatcccgcgc gattcccctg tttttttcag gtactcttca taagtaagat atccaggttg
    13981 ttcattccct cgatgtggaa gaatgaaata gttggtcgta acatctcttt ttataaattc
    14041 ctgcttatag ctttctgctc gttcttttct cccattattc gttgcaccaa aaaatagatc
    14101 cgtctttggt tcgctagaag aaaaattcat atgtttcgaa taaaatgaac tattatgttt
    14161 cagcccaagt tcttttgctt ccaaaaaatc aaaatgataa atctcatcga ttgccggttc
    14221 atcttgtgcc tgcttcagca tcgcctcgtg ttctgctttg aagtgattcc aaaaaaataa
    14281 cacaattttt gcattggtaa atgggcgcac gcgtcgagcc atctccatat caaatccata
    14341 atctaaaaaa acgatcagct gataatttgc caactgttgt ctagctgatt taatgaattc
    14401 cgactgtagt aaatcttttc cggaccaaac atctaaagag cggatggaag gcaaaaaata
    14461 aagtgattta ttttttgcaa ctagcagtac gtcatttttg ttcatctttt ccgcctcact
    14521 ttctctgtca ctttttccta tgatagcctg tttaaatagt aaaaagccag taaaacgttt
    14581 cctatatttg tatgaaaatt taacatttgt atgaaaatat gatgaaataa atcattcttc
    14641 tattaaaatt cgcctaaaag ttttctgaat agttgtaatt gtctgaaaat ttgttatcat
    14701 tagtcattgt taaatattgc tatttattct taatataaat tttgattgtg aatttaaaag
    14761 aaagaggtca tttatggaag ataaaaaatt gaaaaaacaa cttagctcca gacatatcac
    14821 catgttagct ttaggtggag caatcggtgc aggtctattt aaaggaagtg gcgaagcgat
    14881 tgggatcgcc ggcccttctg tattgattgc ttttttgatt ggtgggctga ttttatatat
    14941 cgtgatgaaa ggtctgggaa aaatcgtttt aagcggtggc gacacgcatc acggattatc
    15001 tggtttgata cgcccttatc taggtgctca ttctgctgat tttactgatt gggtctactg
    15061 gtctatgtgg attatcaata tcatcgctga agctgttgca gctgcttcct ttttgcagtt
    15121 gtggttcccg cacattccag cctgggtatt tgtttttatg ttggctgttt taactacgat
    15181 catcaatctt tattcggtac ggttattcgc agaaacagaa tattggctgg cttttgctaa
    15241 aatcagtgtc attatcttac tgatcatttt tgctacttat cttgtcggtc agcaaatgtt
    15301 gggcacaggc gttttcccta cccttcagca attgactgac catggtgggt ttactcctca
    15361 tggtatgaag ggaatcatca gttctttgct tgtagttatc tattcttacg gcggttctga
    15421 actgattgct atcacggtaa gcgaagcaga tgatcctaaa cgtgcgattc ctaaagcaat
    15481 caaaggtgtc atgggaagaa tcatttcctt ttacatcatt cctttgtttt tgcttttgat
    15541 tctttttcct tggaatactt tagcaggcac gaatgtcagt ccttttgtga tggtatttga
    15601 aaaaatgaat atcccatttg cagcggatat cgtcaatttt gtgattgtcc ttgctttgtt
    15661 ctcatctatc aattcgggtg tgtatgcatc ttcaagaatt ttgttcttcc gtttgaagga
    15721 tcgaaaaggt tcctctagaa aactagctgt tttaaacaag catcaagtac cgcaacgtgc
    15781 tgttttattt tgtaccagtg ttctttatct tggtgtagct ctttcttatt ttgtcggtga
    15841 caaattattt ggctatctgg caggttccct ttcgtataca gtattattga tctggatcat
    15901 tatcagtgcg gcatcctttg tactggcact aagaaaagga acattatggg ataaaggaat
    15961 cagctttttt gcattagctg tcttaggatt gattttcctt ggtatcctgt tcacgaactc
    16021 cattggggta actgtattga ctggcctgct ttacctgttc atttatttca gttatcaaaa
    16081 aaagaatgat gcgttcgtat tgacgaatga atgaaatcga tagaaaattt tattttattt
    16141 atgtggaata gatggagcta ttgaagaaag gagttgtgac aaaagtcttg ttgcaactcc
    16201 tttttccgaa taaacagtct catgacctct tggtaaacgg tgttcaaaaa ccctctgtgc
    16261 aaaataattg attgttctcc atgctcatga catgatagaa gaaaggaatg aaaaaaagga
    16321 gtttgtttta aatgaaaaag tattggaagt ttttgttgat gatcggtgtt tcaacggtag
    16381 ttatgtttgg tatgatgtac ttgaatgtct atgcgctcga tcatatcttc ttcagtcgta
    16441 cacgagtatt catggcgttg atgatgggtg cagtcatggc agtcatcatg ctgttgttca
    16501 tgtggaaaat gtatgaaaac aaaaagataa atgttatgat cttagccgct agtcttcttg
    16561 tttttgccgg ttccttgttt atggtacgaa gccaaactac tgtcaacgac acagcttgga
    16621 tgaaggccat gattcctcat cattctatcg ctatcctggc aagtaaacga gctgatttaa
    16681 aagatcctga agtaaaaaaa ttagcggaag aaatcattca ggcacaagaa gaagagatcg
    16741 acaaaatgaa aaaaatgatc aaagaaatgg aatgaccatt atccgtccaa ataaaaaaag
    16801 aggatgttcc agaagttatc agcttcaaga aataagaaga agctacttct ggaacgccgt
    16861 gaataagggg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat
    16921 agcttctcct atttttcgtt tggttaggct tattaccgca gatgcaagct tttgaacacc
    16981 gtttattaag aggttgtaaa atcagcgttc tgacctgagt attagagaga caaatggaaa
    17041 aatagttcct catattttgc cattttttga gctaataccg agggtcagct gattgaacac
    17101 cgtttatcga gaggttgtga aaaagtcgct tagtttcgcg tattaagaaa aattttgtga
    17161 aatggctttc cacatttttg tataaatttg gcttaatacc gagaaactgg cttttgaata
    17221 ccgtttattc gtaaaaggga ttgtgacaaa agtcttgtca caatcccttt ttatgttttt
    17281 attgttctaa ttgcgctgtc ttgcttactg cttcttgcgg tttcacaaat ttttttgaga
    17341 ttccccaacc tgtgaaggca ctgaccatcg aaaacacagg agaaagcaaa ctgaaaaata
    17401 cgaatggcaa gtaagtgagt gtaggaatac caaatgtact tgctacgaaa cttccggcaa
    17461 ttccccaagg cactaagtag ttgattactg ttccaccatc ttctagcgtt ctacctaata
    17521 cagttggatc aaatcctgct ttttcataag tttctttaaa tgcattacca ggcagaataa
    17581 cagataggaa ttgttcacca ataaaaatat ttactccgat acttgtcatc aatgttgtaa
    17641 aaatcaaatt tggtgtgttc gtcatctttc ttgatactcg ttccatcacg gttgagatca
    17701 agccaaattc gatcaacaat ccacctaatg acaaagttag aataatcaat gacacagtag
    17761 gcatcatact ttcgattcct ccgcggctaa gcagtgaatc gatttgttgg tttccagtat
    17821 ttgaaacaaa ccctgattcg atgaccccag ccaacttcgt taaggaagtt tgtgggtttt
    17881 gaataaaaat cattcctact gctacagcga tattcaacaa aatcgttaaa attgccggca
    17941 cctttttcca tgcacacagt aacattaaaa ataaaggaat cagtgaccag attgaaatag
    18001 taaagtttgc ttctagaaca tcagtcactt cacgaataga tgaaagactt gcggctttac
    18061 ttgtgtgtcc taaaatcatg aataaaaaga aagaaacaac aaatgccggg acagttgacc
    18121 acatcatatg cttgatgtgt ttgaacaaat cggcatcaac aatagctgct gctagatttg
    18181 ttgattcgga taatggagac atcttatctc caaaaacggc acctgaaaca atagctccaa
    18241 caaccagcgc tggatttaag cctagtgttg tcccaatacc gaaaaatgca atcccaatcg
    18301 ttgacataac agtaaaggca ctccctacag cgctacctac gattgcacac acaacaaata
    18361 cagaagggac aaaccactgg acactgatca atttgaaccc aatgaccatt agagttggaa
    18421 tgatccctgc ttgtatccat acagcaatca atgaaccgac taaaataaaa ataaagattg
    18481 ggatgattcc tggtttgatg ccattgatga ttccttcgtg cagccaatct gtcgggtatc
    18541 tttttatcac tgcatacaac atgaccattc cgatcgctgc taagatcgtg atattaggtg
    18601 agacctttaa tccgacaacg ccaatcgcaa tgcctaaaac aataaccatt aatacaagtg
    18661 ttgcttctaa aaaacttggt gttccttttt ttcccatgat aatctctcct tttttagttg
    18721 ttcttatgtt ctaaaaaagt taagatatcg gattactccg ccgcaattca tccgcgattc
    18781 ctcctttata aaaataaaaa gacctgtttt aaactaagga cgaatgactc cgcggtacca
    18841 ccttaattaa aaacagatct gtttttcact ttcaaacttt tattcaaatg tatggacttt
    18901 ccatgtaatt cgtcaattag gcttgatcgg ttcacagcga ccaccgactt tctgaacaaa
    18961 acacctaacc gctactgcgt gaaaagttga cttgtacgaa gtttaccttc cataagatca
    19021 tttgtcaatg aaaaagaaaa tattttttca tttttctgaa aattattttt ataactttca
    19081 cacacaagat tattttttac tagaaagtag cgccctcttg tttttttctt gctactattt
    19141 cttgatgttc ttgcttgatt ctttctaata gctttggctc tttcaacaga tcaagagctg
    19201 tattggcgag tagttctgct gcaatcgcaa tagaagcaag gcctttgtca cttttagcag
    19261 cagctttgaa ttcttctgtg tgtccggcaa tatatgtctc agagattgct accgtaggct
    19321 ggattgttgg tatgacttgg ctgacatttc ctacatcaga agaaccgaga cttgttcttt
    19381 gttccgtttc tatttcttca tctggaacac ctgcttcttt tgcatgctgg aagaataagt
    19441 catcaaaaga aggagtaacg atgacatcat caactgcatt ttggaacaat ccaaactcaa
    19501 aagtcgtgcc cgtacttatc gcagcacctc gtacgatatt ttccactttt tcatatactt
    19561 tatctaatgt ttgccgattt cctgcacgta aatagaaacg tccagcagcg taatctggaa
    19621 tgacgttggc agctactcct ccgtctgtaa tgatcccatg gatattgaca tcttttggca
    19681 attgcaagcg tagagcgttg attccattaa acacttggat caatgcttcc aaagcattta
    19741 ctcctttttc aggtgctgct gctgcatggg aagcgacacc gtaaaatttg atgtcgactg
    19801 gatcattcgc taaagattct gtcgttttcc cataacgaaa agctggatgg acgcataacg
    19861 ctacatcgac atcctcaaaa aaaccttctc gaacaaagct gcctttagct gatccgttct
    19921 cgcccccttc ttcccctggg gtgccatata cacggatttc tccaccagtt tcatcaatga
    19981 attgcttcac tatgctggcg gctaatacgg agtaagtacc aaacaaatta tgcccgcatg
    20041 catgaccaat acctggtaaa gcatcgaatt ctgccaaaaa ggcaattgtg ggaccgggtt
    20101 tattggaaat ataacgtgca tcaaaggctg ttcggtgtcc agctacttct ttttctaccc
    20161 aaaacctttc tttttctaat tgtttaatca acgctgctga ggaataaaac tcatagttac
    20221 ttacttcagg atgttcgtgg atatctagag caagtgcttg atattctggt aaacgatatt
    20281 ggattagatc cttcaattgg tttgttgaga ttgttgttgt cataaaaaat tcctcctatg
    20341 ctagtcattt ttcttatttt ctcccgaatg tttcccatgc aggcacgctt gatccttttg
    20401 atgttttctc gattactttg actgtgtctt ctgtttgata agcgtctact attttttgat
    20461 acgttttatt gttttcatct tcttttcttg caacgatgat attcacataa ggacgtgcat
    20521 ggtcatccac tggttctaag aagatggcat cagaagcagg attaaatcca gcatctacag
    20581 ctacaccgct attgatcact gaaatgtcta catctgacaa agcacgtgct gtttgggaag
    20641 catctaattc agaaatgtct agattttttc tattctcggt aatatcagaa acagtgggcg
    20701 cttgtccttt tgccttatcc actttgatca atccagcagt ttgcaataac agaagagctc
    20761 tgccgccatt tgtaacatca ttagggattg ctacttttgc tccgtctttg atatcagcta
    20821 catccttcaa agtgttagaa taaattccca gcggtgcatt gacagtattt ccgatcgata
    20881 ctaaatcagt tccgtgttct ttattgtaat tgtctaaaaa gaattgatgt tggaaagaat
    20941 tcaaatcaat cgatccatct gctaaagcca catttggttg attgtaatct gtaaatttca
    21001 cgtattccaa attaatgttc tcctttttca aacggtcttt cacgttgtcc catacatctg
    21061 tatcttctcc aataatccct agttttacga tagtttcttt tttgtcagtt gtatctgctt
    21121 ttccttggct acaccctgcc aaccaaataa ttgctacaaa tgtaatgact acaaaacgaa
    21181 taattttttt cattttttct cctccacttt caatgtgtaa tttttttaat cagttgatta
    21241 ctgataaatt gacttaaaaa aactaagatt agaatcaata atgtagccac aatcgttaca
    21301 tctgtttgga atcgattata ccctctcgat atagcaagat tccctaatcc gcctgcacct
    21361 actgctccag ccatggtagt cagtccgatc acgttgatga tagtcaacgc cgagacccga
    21421 atgataccag gcaaaccttc taacaaatac acacgtaaaa tgataccgat tggactggtt
    21481 cccatcgctt ctgccgcttc gatcacccca ggatcaactt ctagcagtgc gttctcgatt
    21541 tgacgagcaa aaaacggaac aactccaaaa atcaatggca ctaaagctgc tttcgcacca
    21601 atcgtcgttc caactaagaa acgagtagtc aatgctaaaa gagaaagcaa gataatgaaa
    21661 gggatggaac gaatgatatt gatcagctta tcaagtattt caaatagcag acggttagct
    21721 agtaatccat tagggcgtgt cactactaac gtcacaccta agatgattcc aatcatcccg
    21781 ccaatgaatg ctgtccaaaa agacatatat agtgtttgct tcgtcgcttc gacaaattca
    21841 tccggaatca aataaacatt gggcaaatat gttttaaaga aatcagtcat tttatcttcc
    21901 tccttcgatc actttgatac gactatctaa atcgtttttt tgtaaaatat ttatttttac
    21961 accagatttg atcaaataat ccaatgcttt tttccgctgt ttggtttctc cagctaaagt
    22021 aacgatcaaa ctccctaacg gcgtttcttg taataactct acattgccat ataaaatatt
    22081 cgctgttact tgatatttgc tgtacagatg agcgattaga ggttcgttcg tctgatttcc
    22141 aatataactt aattccacca gccatttatt ggcgatctga tcggcaaaag cagaatgggc
    22201 aatgatcgtt tctaatgctt ggtcaagatg agtagctgtt cgaataaaat ctttggtcaa
    22261 ttcttcttca ggattgctga agatctggat gctgctacct ttttcaacga ttcttccatc
    22321 ttccataacc gctaccttgt tgcagatttc tttgacaact tgcatctcat gagtgatcag
    22381 gacgattgtt aaacctaact ggcgattcaa tttttttaac aatgcaagga tctgaagggt
    22441 cgttttcgga tcaagtgcac tggttgcttc atcacataat aataccttag gttcattcgc
    22501 caaagctcgt gcgattgcta ctcgttgttt ttgtcccccc gacagttgtt taggataagc
    22561 agatgctttt tcttccaaac cgacaagttc cagtagttca ttcacttttt gacgacgttc
    22621 ctgtttcgat tttccagaat actttaaaga aaaatcaacg ttgtcaaaaa tagttcggct
    22681 ttccattaga ttgaaatgct gaaagatcat accgatggcc tttctttctt ttcgcaattc
    22741 tttgggagag agctgactca gattcgtctg attgacaatg acttctcctg ctgttggttt
    22801 ctgcagcaaa ttcatcacac gaactaacgt acttttccct gcacctgagt aaccgacgat
    22861 tccataaaca tcgccttttt caatcgttaa atctacagaa tctactgcct gtatctcttt
    22921 ttctttttgt ttgaaacata cagaaacatc tcgcaattca atcattaaaa ttcctcctta
    22981 tctaaaaaaa gcgtcctcaa gaaaatgaaa atgtcatttt cttgaggacg cttttttgcg
    23041 tgttaccacc tcatgttcaa gctgtgttca cacacaactc ctcttcaagt acgatcatac
    23101 tctagcaaga tatcggtcgc agccgtcatc gccttacatc tatgctcggg atgaattgct
    23161 cgatggccat tttccaatag tcgttccttg cttcttctca gctaccgaag ctctctgtca
    23221 aaggttcttc tatcgtactt accatttcaa tgcaaataat attcaaaaaa aaagtccttt
    23281 tactaacata aactatgtca gtaaaaggac gacgtatcgc ggtaccacct tttttcggaa
    23341 gtttccttcc ctcaagccca acatctcaaa aagagagatt ggcgttcgtt ttaacggtcg
    23401 aacagttccg aatttgccta cttatcgaca aattattctc caaggccatt ttcagctttt
    23461 cttacttgac tcttctcacc agccagagcc tctctgcaaa gtttccaaac ttactttcct
    23521 tttcaacgaa atcgatattt tgttgttggg attaatctac atgaggaaaa ctgaaaagtc
    23581 aagtacgaaa ttcaaattat tttttctata tttatttttc tgttttttta tttaacattt
    23641 caataacagt tcgaagatct tttccttccg caagaagttt caatttttcc ttttcttcga
    23701 cggtataatt tcggacaaaa ctggaatcag gtatcaaaac aacgcctaat cctgctcgtg
    23761 aagcagctgt tgctcctact aaagaatctt ccactgcaaa ggctgaacta gcttctaatc
    23821 ctgttcgttc caaagctgtc agataaggaa tcggtgaagg cttgttctcc gacacttcgt
    23881 ctccgaatgt aaacgtgtcc aagtaatcag ctaatttaaa gtgatccaag atatctgttg
    23941 ctcttttcgt aacagtggtc gtagcaatgc ctagtttcat atgacgttct tttaagtctg
    24001 ccaatatttc ttttgcatat ggttttaagt ggatggctcc gcttgtcagc tgttgataaa
    24061 aatattcttc acgcttcgct cggacttcct gtactttttc agggctacct gctgctttca
    24121 ctaaaatatc aaaactttgt ttccagtttt ttccgcccca agcgtcaacg atttcttttg
    24181 ggatatggat atcaaattgc tgaaatcccc aaagccagcc atcacgatac gttttttctg
    24241 tgtcaactaa caaaccatct aaatcaaata ttgcaccttt catctataca ttaatccttt
    24301 ccaaaacaat acacatagtt tagcacgact ggtccaaaaa gaacagaagc aaagaaccag
    24361 ccttctcgtt tataagagtt cttggaatcg acggatatag attttcttca cagctgtcac
    24421 aagcgccaga taagcgatga ctgtagcaaa taaccataac cagtaattgc caggaagtgg
    24481 catcaagcct aattcagcac caaaacttgt aaaaggaagg acagaaccga caataattcc
    24541 taatgtagtg ataacaaaca tggcaaatgt tgcgttgctt tggataaatg gtactttcgg
    24601 tgtacgcagt gcatggagca ctaaggtttg tgtccatagt gattcgacaa accatcctgc
    24661 atggaataat gcgatgaaaa tcactcgctg tgaactatcc aatgtatgga aacttccgcc
    24721 cactacagca ggacaaataa caaaatacat caacaagtaa gtagtaatat caaatatcga
    24781 acttgttgga ccaaagtaaa ccatgaattc cccaatacta gatgcattcc atttttttgg
    24841 ctcgtccaaa tattctttat ccatccgatc ccatggcaag gaaatgcagg aaacatcata
    24901 gatcagattc aaaaacaaaa gttgtaaagg cagcattggt aaaaatggca ggaacgtact
    24961 tgcaatcagc acggaaaaca tattgccaaa attagaactt gctgtagcct tgatatattt
    25021 catgatattc ccaaaagttt ctcgtccaga aagaaggcct ctttccaaaa tcgtcaaatc
    25081 tttttctaaa aggatcacgt ctgcagattc tttcgcaata tccacagctg tatctacaga
    25141 aataccgata tcagccactt tcattgcagg agcgtcattg ataccatctc ctaaaaatcc
    25201 gacagtatga ccatttttac gtaaagctgt cgtcaaacgt gctttttgct gaggattcaa
    25261 ttttacgaat acttcatggt cttcagctac ttgtgctaat tcatgatcac tcatttccga
    25321 gattttctca ccggtaacca attcattgat ctctagacct acttgacggc acactgaacg
    25381 tgtaactaat tcgttgtccc cagtcagaac ttttacagct acaccatgtt ctttcagagc
    25441 tttcaacgct tgtttcgttg tttcttttgg tggatcaagg aaagcaagat aaccaatcaa
    25501 aaccatgtct gactcatctt tgactgaaaa ttctccaacg acgcttggat tggtcttttg
    25561 tgcgacacca atcactcgta agccatcttc attaagtgac tgtacttttg caagtacggc
    25621 ttcttttcgt tcttttgtta atggtgtaac agtaccgcca agatcaataa atttagaaat
    25681 gttcagcatt tcttcaatcg caccttttgt gatcatttgt gttttaccag ctggatcagc
    25741 aaccacaaca ctcatacgac gacgttcaaa atcaaaagga atttcatcca ctttttgata
    25801 attgatctta tctgtatcta aagtttctgt tgcttcatca atgatagcca catctaacaa
    25861 atttttcagc cctgtttgat agtagctatt tagatacgca tggcgcaaaa cacgatcatc
    25921 ttcatttcct tcacaatcca tatgatattc aagaatgatt ttatcttgtg tcaatgtccc
    25981 cgttttatcc gtacataaca catcgattgc accaaagttc tggatggcat tcaaattttt
    26041 gatcactgtt ccttttttgg ccatcacact tgctcctttg accaaattcg tcgtaacgat
    26101 cattggaagc atttcaggtg ttagaccaac tgctacagat agtccaaaaa gaaaagcttc
    26161 tagccaatcc ccttttgtaa gcccattgat gacgataact gctggagcca tcaatgacat
    26221 aaatttgatc agtagcatgg atgttttatg gatgcctaat tcaaagcttg attgaattgg
    26281 tttttcagat agcgtttctg ctacatgacc aaatagtgtt tcatttccaa cagaaacaat
    26341 gattccttcc gcacttccac tgatgacatt acttcccata aagacaagat tttcgtaact
    26401 tgtttcgctt tgctgatcgt cttcacatac tttcgcatgc ttttctactg gatagctttc
    26461 ccctgtcaat gctgcttggg aaatgaataa atcctttgcg gacaataaac gcatatccgc
    26521 tggtatcata tcccctgctg aaagccgaac aagatctcca cagacgattt cttccatagg
    26581 aatctctgtg tactttccat tacgttttac agctgccgtt actttcacaa gtgatttcag
    26641 tttttcagcc gcttgattgg aacgtacgga ctgaatcaac gtcattgtcc cgctgacgat
    26701 aaccatagtg aagataatgg ctacaccgaa aagatcttta tctccaggag ctgctaatac
    26761 atattccgtg atgaatgaaa taattcctaa tgcgatcaac actaatgtaa atggcgtgat
    26821 gtatgctttc aatatttcta caatgaatgg tgtttttttg ccatatgaaa tcgcattttc
    26881 accgtaattt tctcttgaac gttcgatttg ttcttctgtc aatccatttg ctgatgcatg
    26941 gaatagttca aataattctt gtttcgtttt tccagcaaaa gctgcataga ttttttgtgt
    27001 atctttcttt ttttccatct caatctctcc tttgtgtcca aaagacagct gttctcgctg
    27061 cttttggtct ttagctattc atcggatcga tagagaaaaa actgaaaggg gctgagaaca
    27121 gatagataat agccctggcc tttcctctag atccttgttt ttcgaatgac tgtcatccat
    27181 ttgttctcat ctcctttact aagtaatttt aattcatgac ttccaatcat ttttcgaata
    27241 gttatttttc caattagacc acctcctttt tatttttcac cgatggggat ttgcggcaac
    27301 aaaaaaacct ctcgccaaat actcagcgaa aggtttgaaa aagcatatta aaaaaagctt
    27361 ctcagatctc atcgttgagt tttgacactg tacaacgtaa aaagcagata cctgctttta
    27421 gctaccttat gaaaagcctt atctcgacaa ttcctgttct acccattggc gtctctcgac
    27481 gtttttgggc agtagcctat gtttgtatag gagcctcacc taacaaatct tattcggttt
    27541 tccttcgtta atataacaac taaaaaagag gatgtcaaat caattttcca tgtttttcat
    27601 acttttttta aaatacacac gttatattcc tgtctcacgc ggttttattt gtctcatttt
    27661 ccacctcccg ctataataga aatatagtag gaaacgaggt gctgttatgg aaagaacact
    27721 tgttattatc aagccagacg gcgtgagacg ccatttagtc ggaagcatca tccaacgttt
    27781 tgaagcaaag ggactggcca ttgctgaaat gaagtttgaa actatgactc ctgaattagc
    27841 aaaagaacat tatcagcact tgactgagcg cagttttttt gatgaactga tcgattacat
    27901 gacttccggt cctgtcgttt atcttgtgct ggtcggcgag gaagtgatcg atatcgtccg
    27961 taagatggtt ggagcaacaa aagctgccga tgctgttcca ggtacgatcc gaggagacta
    28021 cgcgctgccg gggactgaaa acatcattca cgcttctgat tcccgtgatg ctgcggtaaa
    28081 agagatcgcc cgctttttcc cgccattcga tataaacaaa ccgtctgaaa aagcggcgaa
    28141 gtgatacaaa ttctaaactg ctggtctagt tgctgggttc agcaatggcc agcagttttt
    28201 tctctaagga cctccgttga ttctttccta acatatattc gagcactgtg aagaattctt
    28261 ggtagtcttt cgtcttttct tcccctaaag cagcagaaat caatctttga taataatctg
    28321 ccatcaatgt ttcatagatc actgtacttc tttctttatg tgctaagctc cgtttcatat
    28381 cttcattcac actacgtgcc tttttcaacc gaccattttc tatataaaac gaagtcaggt
    28441 tcattaaaaa agtaaagaga tactgctcat gattgttcaa ctgtgctaat cgtcgcattc
    28501 tttttttgat tttcaaataa ttgttctcaa tgaaccgatc actgaatatc caaggaaaat
    28561 cagctagaat agatagttca aattgaccat agtctggtat ctgcatcaag tattcttcaa
    28621 tgattcggca ttcataacgt aaaaattcaa attctgtctc agatacttct gttttttcga
    28681 ccatgtgaag attcaaaacc aaataataca gatcttctgt tttcttaaaa tgcctacggt
    28741 tattgctgat ctctttataa ggatcaaagc tgtcttcttg tacccgatct agcattcgtc
    28801 tttttttctt taactttatt ggttcatcca gctggtttcg catccattca tattctgcaa
    28861 gtgtcatgtt cattcgctct aaaaatgatc tcaatgttac aaatgacatt tttttcactg
    28921 aatttgtctc aatcccgact agagaagtct taaaacagat tccttccgct aattgggact
    28981 gtgtcaactt cttgttgact cgaagtttct tgatcaattc accatcatac ataatagttc
    29041 tcctttttaa tttaaatttt tccacattca ataaatagat acgttaaaaa ggaaaaatac
    29101 acgaaaaaaa taa
//
LOCUS       DAYYHX010000010        29097 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000010 DAYYHX010000000
VERSION     DAYYHX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.010"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACHA6Y_000329"
     CDS             complement(<1..1031)
                     /locus_tag="ACHA6Y_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8986452.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACHA6Y_000330"
     CDS             complement(1113..1802)
                     /locus_tag="ACHA6Y_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8986453.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHA6Y_000331"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHA6Y_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM8986454.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHA6Y_000332"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHA6Y_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM8986455.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHA6Y_000333"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHA6Y_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM8986456.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHA6Y_000334"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHA6Y_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM8986457.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHA6Y_000335"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHA6Y_000335"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM8986458.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHA6Y_000336"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHA6Y_000336"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM8986459.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACHA6Y_000337"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACHA6Y_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACHA6Y_000338"
     CDS             complement(7636..8523)
                     /locus_tag="ACHA6Y_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8986460.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHA6Y_000339"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHA6Y_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM8986461.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHA6Y_000340"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHA6Y_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM8986462.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHA6Y_000341"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHA6Y_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8986463.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHA6Y_000342"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHA6Y_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM8986464.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACHA6Y_000343"
     CDS             complement(10454..10957)
                     /locus_tag="ACHA6Y_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8986465.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACHA6Y_000344"
     CDS             11554..12519
                     /locus_tag="ACHA6Y_000344"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8986466.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACHA6Y_000345"
     CDS             complement(12618..13790)
                     /locus_tag="ACHA6Y_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8986467.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACHA6Y_000346"
     CDS             complement(13777..14922)
                     /locus_tag="ACHA6Y_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8986468.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACHA6Y_000347"
     CDS             complement(14936..15868)
                     /locus_tag="ACHA6Y_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8986469.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACHA6Y_000348"
     CDS             15985..17064
                     /locus_tag="ACHA6Y_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8986470.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="ACHA6Y_000349"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="ACHA6Y_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17789..18016)
                     /locus_tag="ACHA6Y_000350"
     CDS             complement(17789..18016)
                     /locus_tag="ACHA6Y_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8986471.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="ACHA6Y_000351"
     CDS             complement(18119..20707)
                     /locus_tag="ACHA6Y_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM8986472.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="ACHA6Y_000352"
     CDS             complement(20727..21386)
                     /locus_tag="ACHA6Y_000352"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8986473.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHA6Y_000353"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHA6Y_000353"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM8986474.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="ACHA6Y_000354"
     CDS             complement(22159..23025)
                     /locus_tag="ACHA6Y_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8986475.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="ACHA6Y_000355"
     CDS             23134..23775
                     /locus_tag="ACHA6Y_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM8986476.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHA6Y_000356"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHA6Y_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM8986477.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="ACHA6Y_000357"
     CDS             complement(25216..26115)
                     /locus_tag="ACHA6Y_000357"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8986478.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="ACHA6Y_000358"
     CDS             complement(26117..26914)
                     /locus_tag="ACHA6Y_000358"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM8986479.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="ACHA6Y_000359"
     CDS             complement(26892..27596)
                     /locus_tag="ACHA6Y_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM8986480.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="ACHA6Y_000360"
     CDS             27727..28302
                     /locus_tag="ACHA6Y_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM8986481.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="ACHA6Y_000361"
     CDS             28420..29097
                     /locus_tag="ACHA6Y_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM8986482.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8561 a         6167 c         5231 g         9138 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaa
//
LOCUS       DAYYHX010000011        28968 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000011 DAYYHX010000000
VERSION     DAYYHX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.011"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACHA6Y_000362"
     CDS             147..1016
                     /locus_tag="ACHA6Y_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8986483.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACHA6Y_000363"
     CDS             1225..2250
                     /locus_tag="ACHA6Y_000363"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8986484.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACHA6Y_000364"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACHA6Y_000364"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM8986485.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3333..5036
                     /locus_tag="ACHA6Y_000365"
     CDS             3333..5036
                     /locus_tag="ACHA6Y_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8986486.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACHA6Y_000366"
     CDS             5038..7071
                     /locus_tag="ACHA6Y_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM8986487.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACHA6Y_000367"
     CDS             7082..7522
                     /locus_tag="ACHA6Y_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8986488.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACHA6Y_000368"
     CDS             complement(7722..8849)
                     /locus_tag="ACHA6Y_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8986489.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPITEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACHA6Y_000369"
     CDS             complement(8852..9529)
                     /locus_tag="ACHA6Y_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8986490.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACHA6Y_000370"
     CDS             9793..11559
                     /locus_tag="ACHA6Y_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM8986491.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACHA6Y_000371"
     CDS             11574..12011
                     /locus_tag="ACHA6Y_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8986492.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACHA6Y_000372"
     CDS             12024..13175
                     /locus_tag="ACHA6Y_000372"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8986493.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13237..13935)
                     /locus_tag="ACHA6Y_000373"
     CDS             complement(13237..13935)
                     /locus_tag="ACHA6Y_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM8986494.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13928..15280)
                     /locus_tag="ACHA6Y_000374"
     CDS             complement(13928..15280)
                     /locus_tag="ACHA6Y_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM8986495.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15503..16903)
                     /locus_tag="ACHA6Y_000375"
     CDS             complement(15503..16903)
                     /locus_tag="ACHA6Y_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8986496.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17064..17801
                     /locus_tag="ACHA6Y_000376"
     CDS             17064..17801
                     /locus_tag="ACHA6Y_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM8986497.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17926..18405
                     /locus_tag="ACHA6Y_000377"
     CDS             17926..18405
                     /locus_tag="ACHA6Y_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM8986498.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18402..19169
                     /locus_tag="ACHA6Y_000378"
     CDS             18402..19169
                     /locus_tag="ACHA6Y_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM8986499.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19399..19968
                     /gene="lepB"
                     /locus_tag="ACHA6Y_000379"
     CDS             19399..19968
                     /gene="lepB"
                     /locus_tag="ACHA6Y_000379"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8986500.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20072..20674)
                     /locus_tag="ACHA6Y_000380"
     CDS             complement(20072..20674)
                     /locus_tag="ACHA6Y_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8986501.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20780..21010
                     /locus_tag="ACHA6Y_000381"
     CDS             20780..21010
                     /locus_tag="ACHA6Y_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986502.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            21001..21249
                     /locus_tag="ACHA6Y_000382"
     CDS             21001..21249
                     /locus_tag="ACHA6Y_000382"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8986503.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            21355..21939
                     /locus_tag="ACHA6Y_000383"
     CDS             21355..21939
                     /locus_tag="ACHA6Y_000383"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8986504.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21942..23015
                     /gene="prfA"
                     /locus_tag="ACHA6Y_000384"
     CDS             21942..23015
                     /gene="prfA"
                     /locus_tag="ACHA6Y_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM8986505.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23008..23847
                     /gene="prmC"
                     /locus_tag="ACHA6Y_000385"
     CDS             23008..23847
                     /gene="prmC"
                     /locus_tag="ACHA6Y_000385"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM8986506.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23840..24019
                     /locus_tag="ACHA6Y_000386"
     CDS             23840..24019
                     /locus_tag="ACHA6Y_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986507.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24041..25066
                     /locus_tag="ACHA6Y_000387"
     CDS             24041..25066
                     /locus_tag="ACHA6Y_000387"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM8986508.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25257..26501
                     /gene="glyA"
                     /locus_tag="ACHA6Y_000388"
     CDS             25257..26501
                     /gene="glyA"
                     /locus_tag="ACHA6Y_000388"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM8986509.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26654..27601
                     /locus_tag="ACHA6Y_000389"
     CDS             26654..27601
                     /locus_tag="ACHA6Y_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM8986510.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27735..28364
                     /gene="upp"
                     /locus_tag="ACHA6Y_000390"
     CDS             27735..28364
                     /gene="upp"
                     /locus_tag="ACHA6Y_000390"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM8986511.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28503..28745)
                     /locus_tag="ACHA6Y_000391"
     CDS             complement(28503..28745)
                     /locus_tag="ACHA6Y_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986512.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28866..28938
                     /locus_tag="ACHA6Y_000392"
     tRNA            28866..28938
                     /locus_tag="ACHA6Y_000392"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28899..28901,aa:Thr,seq:cgt)
BASE COUNT         9842 a         5119 c         5930 g         8077 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatcca gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcagtg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttcgct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata
    13261 gggcagatcc aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc
    13321 caggttctca tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta
    13381 aatgacacct tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa
    13441 aggtcgttca ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt
    13501 ttcattgaat tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact
    13561 taacgtgtag tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta
    13621 gtagtgacct agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat
    13681 atagttgatc aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa
    13741 gtcttgacca ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat
    13801 actaacgatc tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa
    13861 aaggtttccg ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag
    13921 ttcataatta gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac
    13981 gaagctgcaa tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt
    14041 tgattttttc gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc
    14101 cggcaacttt tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg
    14161 ggatatccat atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc
    14221 cgtctgcgta attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa
    14281 tcatatcgat tacttgattg atacctacag gatttttgac aagtactaat gtacattttt
    14341 tcccattgat attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt
    14401 gtttgatttt atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat
    14461 tgtagacatt atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat
    14521 ctgctgacac attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt
    14581 tgaagtcaca atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat
    14641 gcaagatgtg atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt
    14701 cacgatcttc ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat
    14761 tgaagattgg tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt
    14821 ctacaatcat tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa
    14881 tatttgtaaa taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt
    14941 catctatttc taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag
    15001 tcgatacaat tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt
    15061 gctggcgcaa aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga
    15121 caacgacgac ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg
    15181 ctagtttacc tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg
    15241 tttttccaac tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga
    15301 tttgctgacg aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt
    15361 ttcttattct taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga
    15421 tttaacaaaa aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt
    15481 ccctcttacc tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc
    15541 ctagatctcg tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc
    15601 caataaacat cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc
    15661 ctaaagataa accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg
    15721 tttgttcttc tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca
    15781 taaagacaac gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga
    15841 tgttatcttc acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg
    15901 ttcgaatccc gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa
    15961 tgatcagtgt tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag
    16021 tcgtaatcga ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata
    16081 aatggtcctt ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag
    16141 ctaacacagc taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt
    16201 tgtataatag ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa
    16261 tcaaaaacat gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc
    16321 cagtgacgtc aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa
    16381 cggcatcacg ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc
    16441 caaaaagtaa ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac
    16501 cagacaaacg cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc
    16561 gtcgtttaga aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt
    16621 gaaataatat ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag
    16681 tcgatagtcc agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa
    16741 catggctatc tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca
    16801 tcaatatata ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct
    16861 gtcctcgccg ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac
    16921 aatagtcgtt tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca
    16981 gataacaata cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa
    17041 aaagaaggag gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa
    17101 atcaaaaaat aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag
    17161 aagtaaagcc taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg
    17221 aattcatgtc tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta
    17281 aaagtgagta tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga
    17341 aaactgacca tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa
    17401 tggttttaac aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct
    17461 tcaatatgcg attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg
    17521 gaagtgccct gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg
    17581 atatccaaaa gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct
    17641 atgaacgctc tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt
    17701 gcgtgttcgc tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag
    17761 cttacttaaa aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac
    17821 gtaagaaaaa aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag
    17881 taaagtaagg aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca
    17941 cctattttga agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt
    18001 attgtgaata gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca
    18061 acaccaacta cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc
    18121 actacgacaa gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca
    18181 actgacagct caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc
    18241 aatccaagta caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact
    18301 gtagatgata acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca
    18361 ggaaataccc aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc
    18421 ttatcaacgg ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc
    18481 gtggatatac gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac
    18541 tttggcaaaa ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg
    18601 tttattttac tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg
    18661 tcactcagct ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat
    18721 cgatagataa tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg
    18781 acgaagatta tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga
    18841 tcaaaacaaa tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc
    18901 cttattacta tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct
    18961 actcggacat ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac
    19021 ctgtcggatt gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc
    19081 cttatcgtct gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata
    19141 gtcgtagcca aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg
    19201 ataaaaacgt ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt
    19261 atataacgag aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt
    19321 gtagaataaa gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat
    19381 acaagtagga ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta
    19441 ttagcggcta ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat
    19501 tcgatggacc ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat
    19561 gaacgaatgg atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa
    19621 cggatcatcg gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat
    19681 ggcaaaaagt atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag
    19741 ttacaaggag aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc
    19801 tttacgagtg actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac
    19861 cgcttgattt caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga
    19921 aaagttgttt tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa
    19981 aagaagctgt gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct
    20041 tggagttgct cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa
    20101 gcgaagcgtt tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag
    20161 agacaaagcc cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga
    20221 ctttcggatg actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag
    20281 gagctaaaat ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta
    20341 caagtgatag aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt
    20401 ggatcgttcg gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca
    20461 tagtttctga agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa
    20521 caagcccagt tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat
    20581 aagtagttag ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat
    20641 ggttagcgat ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg
    20701 aaattttaac ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa
    20761 gggagaatct agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa
    20821 gtgtgtccga gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga
    20881 aaaatatatc gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata
    20941 tttaggcttt atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt
    21001 atgggaataa aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca
    21061 gaaggttacg cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac
    21121 tactctatat atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa
    21181 gtttttccaa atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa
    21241 caaaagtgag caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa
    21301 atgctgaaaa tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca
    21361 caactatttt ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc
    21421 gcacataatt atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca
    21481 agagatggtt taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata
    21541 tttgaagaaa cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg
    21601 ctgatcgatg aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg
    21661 gacgaattgg atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta
    21721 tttgaaggat caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc
    21781 tgttggttct gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt
    21841 tatgaaggcg atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa
    21901 cattactttc acccatcaat gattacagaa ggagaataag catgtacgat caattacaag
    21961 caattgaaga ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg
    22021 atacaaagcg ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag
    22081 tttatcgtcg ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg
    22141 aaaaactgga tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag
    22201 aaaaagaagt cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg
    22261 ataaaaatat catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg
    22321 ctggcgatct tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag
    22381 tgatggaagc taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg
    22441 gagacaacgt atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc
    22501 cttcaactga atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag
    22561 aagctgaaga agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg
    22621 cttcaggtgc tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact
    22681 taccaacagg gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa
    22741 aagcaatgaa aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg
    22801 agtatgatgc caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga
    22861 cgtataattt cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat
    22921 tagatcagat tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc
    22981 aaacatcaaa actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca
    23041 cgggcttctt cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt
    23101 ttggaacgaa aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca
    23161 gaagaagaac agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct
    23221 cagtatttgc taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca
    23281 ctgatccctc gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt
    23341 gtaccgttag aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa
    23401 gcagcaagaa aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta
    23461 gcgaaagaaa atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta
    23521 ggaccagtaa tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg
    23581 aatgaatggg aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt
    23641 gctgagaacg atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta
    23701 aaatcaaatg gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa
    23761 atatttcagc aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat
    23821 gaacgaatga tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt
    23881 tattataaaa aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt
    23941 ttatccacta ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag
    24001 acaaaataag aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga
    24061 tgaaacagaa ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc
    24121 taccgaaaca gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt
    24181 gttttctgta aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga
    24241 acaagtaaaa gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt
    24301 ctggcctgga ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt
    24361 gacgggagga ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt
    24421 aatcgaactt tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag
    24481 tccaacgact gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga
    24541 tgatggagct acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc
    24601 catgcctatg attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga
    24661 atctcctgtt gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg
    24721 gatgaaatac aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg
    24781 gtcaactgca gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc
    24841 cgaaattgcg gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc
    24901 agtagaagcg gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact
    24961 tggattagat ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat
    25021 gaatcgcttg aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg
    25081 tcgcattctt ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa
    25141 ctaagcgtaa ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg
    25201 aaaaataaaa ttgtaggaaa ataaaattgt aggaaaataa aaaggagctg ttgtttgtgg
    25261 tagattacaa aacgtttgac cctgatcttt gggctgcgat cgccaaagaa gaagaaagac
    25321 aagaacataa tttggagctg attgcctctg aaaactttgt ctccgaagca gtgatggctg
    25381 cacaaggaag tatcttgaca aataaatacg cagaaggtta tccagggcat cgctattatg
    25441 gtgggtgtga atttgtcgat atcgttgaaa atttagcgat tgaccgtgca aaagaattat
    25501 tcggagcgaa atttgcgaac gtccagcctc actccggctc tcaagccaac acggctgctt
    25561 atctcgcttt ggtagaacca ggggacacga tcttaggaat ggatctttcc gcaggcggac
    25621 atctaacaca tggatcacca gtcaacttca gtggcaaaac ctatcacttt gttgcttacg
    25681 gtgttgatcc gacaacagaa gttattgatt ataatgtggt acgtatttta gctagaaaac
    25741 atcaaccaaa attgatcgta gcaggtgcca gtgcttacgg acgaacgatc gactttgcga
    25801 aattcagaga aatcgcagat gaagtaggcg caaaactgat ggtagatatg gcacatatcg
    25861 caggattagt agcagcagga cttcatccta atccagttcc ttatgcagat atcacaacga
    25921 ctaccactca taaaacattg cgtggccctc gaggcggcat gattttgaca aacgatgaag
    25981 cattagcaaa aaaaataaat agtgcggtat ttcctggtat tcaaggcggc cctctagagc
    26041 atgtgattgc gggtaaagca gtagcattca aagaagcatt agatcctgca tttaaagaat
    26101 atagtgaaca aatcatcgcc aatgcaaaag caatggtcaa agttttcaat caagcaattg
    26161 gaaccagagt catttcaggg gcgacagata atcatctgat gttgatcgat gttcgcgagt
    26221 taggtataaa tggaaaagaa gcagagagca ttttggacag tgtgaatatc actgtaaata
    26281 aaaactcgat tccttttgaa acactaagtc cattcaaaac aagcggtatt cgaatcggca
    26341 cgccagcaat cactactcgc gggttcaaag aagaagatgc tgtaaaagtg gctgaactag
    26401 ttgtcaaagc attacaagca aaagatgata acgctcaact agacgaagtc aaaacaggtg
    26461 tacgcgaatt aactgaaaaa tttccgttgc ataaaaaata aaaatttaaa ttccattagt
    26521 aaacagaaaa gttcaatgtt gggcttttct gtttattttt tgggaaaacg gattctttga
    26581 caatatcggt gatgctctct agtatatgag gtgtaatttt agtcttcaca ctaaatctca
    26641 gggaggaaaa gtcatgactg atcgtatctc gttagaaaag gaagcagtag cattcagtga
    26701 tgcaaatgcg cctcatccgc gtatctatga actagcaccg gaagaaggaa gagttttact
    26761 agaagaagta caagaatcac ctgtgaacaa atattccgtc gatattgaag acttgatggt
    26821 ggatactggc gaatggggaa aactcaatat tcgtctcgta cgaccagaag ggaatatgga
    26881 taaattacca gttatttttt atattcatgg agcaggctgg gtttttggca gtgctcaaac
    26941 ccatgataaa ttgatcagag aacttgcggt tcgtacgaat tccgtcgttg tctttccaga
    27001 atatagtcga tcaccagaag caaaatatcc aacagcaatt gaacaaaatt atgctgtgtt
    27061 gcaacagctg aataaattag ccaagtcaaa aaatctggat ctcgagaatc taatcgttgc
    27121 aggagattca gtaggtggta acatggctac agtcatgacg atcttaacaa aagaacgaaa
    27181 gggcctccca atatccaagc aattgctgta ttatccagtc acaaacgctg attttgatac
    27241 agattcctat catgaattcg gagaaaacta ttttttgaca agagaaggaa tgatgtggtt
    27301 ttgggaccaa tatacgacga atgaagaaga gcgtgctgaa atcacagctt cgcctttgag
    27361 agcaaatata aatgaactaa atgatcttcc ttcagcaatg attttaacgg gagaagcgga
    27421 tgtattaaga gatgaaggtg aggcttatgc gcgcaaactt cgtgatgcag gggttcctgt
    27481 tacacaagta cgcttccaag gaatcatcca tgattttgtc atggtaaatg ctatggatca
    27541 gacaaaggca acgcgtgcag caatggcatt atctacttct tggatcaaga aaaaagaata
    27601 agctttgctt gtccgcagga aaacttcttt tcaaaaacgg attttcttag gaaaaatggc
    27661 gagtttgact agtcaatcac ttgattttgt tttacaatta aaaagaaacg acagaacaaa
    27721 ggagagctta aaaaatgggc aaatttcaag tgatcgatca tccattgatt caacacaaac
    27781 taactattat tcgggacaaa aactgcggaa caaaggtttt tcgtgaagta gtcgatgaaa
    27841 tcgctatgtt gatggcatat gaagtatcac gggatatgcc attggaagac atcgtgatcg
    27901 aaacaccgat cactgaaaca acacaaaaaa cgttgtctgg taaaaaagta gcgattattc
    27961 caatcttgcg cgcaggtatt ggaatggttg acggtatctt ggaattgatt ccagctgcta
    28021 aagtgggaca tatcggtctt tatcgagatg aagaaacatt ggaaccacat gaatatttcg
    28081 tcaaacttcc agaagacatt gatgcacgtc agttgtttgt agtagatcca atgttggcaa
    28141 caggcggatc agcaatcatg gcaatcgatg cattgaaaga acgtggcgcg tcgaacatta
    28201 aatttgtttg tttggtagca gcacctgagg gagtaaaagc tttgcaagaa gcgcatcctg
    28261 atattgacat ctatacagct tctctagatg aaaaattgaa tgaacatggt tatatcgttc
    28321 ctggactagg ggatgcgggt gaccgtttgt ttggtacaaa ataagtaaat acgaaaaagg
    28381 gtgtgacaca acttttgtca catccttttt tggtcatttt taacgtttga tgcagaacag
    28441 ctttttcaca gctttcgttt gcatataaaa aaaccatccc aaaaatgcag agatggtcaa
    28501 attcaaatct tttttagttc tttgtagtta acgattacta cacccttact ttgcgtcatc
    28561 aagttcgcat tttctgcagt ttcatcaatc tcaacaacag cggaattttt caattgcttt
    28621 gaaatcgttc cagtttgcga cttgccgtga attgtgaaca ttacgtgagt attgatcttg
    28681 aatttttcac caatttccga tggtacgata gttgtatcaa attttccaaa agttgccatt
    28741 ttcataaaac ctgcctttca catcaattat accacgtttt atgaaaaata tcatgaaatt
    28801 aaactaattt tccatcggac tctttacaat cagccaaata gagtatataa tttcttttgg
    28861 ataaggccag cttagctcag ttggtagagc aacgcactcg taacgcgtag gtcgtaggtt
    28921 cgatccctat agctggcatt aaaagagaag acagatatat caaggatt
//
LOCUS       DAYYHX010000012        28688 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000012 DAYYHX010000000
VERSION     DAYYHX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.012"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..677
                     /locus_tag="ACHA6Y_000393"
     CDS             108..677
                     /locus_tag="ACHA6Y_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8986513.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(855..1295)
                     /locus_tag="ACHA6Y_000394"
     CDS             complement(855..1295)
                     /locus_tag="ACHA6Y_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8986514.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1453..2217
                     /gene="map"
                     /locus_tag="ACHA6Y_000395"
     CDS             1453..2217
                     /gene="map"
                     /locus_tag="ACHA6Y_000395"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8986515.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            2249..3172
                     /locus_tag="ACHA6Y_000396"
     CDS             2249..3172
                     /locus_tag="ACHA6Y_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8986516.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(3397..3636)
                     /locus_tag="ACHA6Y_000397"
     CDS             complement(3397..3636)
                     /locus_tag="ACHA6Y_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8986517.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(3641..4060)
                     /locus_tag="ACHA6Y_000398"
     CDS             complement(3641..4060)
                     /locus_tag="ACHA6Y_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8986518.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(4287..5525)
                     /gene="mef(H)"
                     /locus_tag="ACHA6Y_000399"
                     /gene_synonym="mefH"
     CDS             complement(4287..5525)
                     /gene="mef(H)"
                     /locus_tag="ACHA6Y_000399"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8986519.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(5632..6267)
                     /locus_tag="ACHA6Y_000400"
     CDS             complement(5632..6267)
                     /locus_tag="ACHA6Y_000400"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8986520.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(6381..6473)
                     /locus_tag="ACHA6Y_000401"
                     /pseudo
     CDS             complement(6381..6473)
                     /locus_tag="ACHA6Y_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(6609..7244)
                     /locus_tag="ACHA6Y_000402"
     CDS             complement(6609..7244)
                     /locus_tag="ACHA6Y_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8986521.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            7756..8898
                     /locus_tag="ACHA6Y_000403"
     CDS             7756..8898
                     /locus_tag="ACHA6Y_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8986522.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            8891..9097
                     /locus_tag="ACHA6Y_000404"
     CDS             8891..9097
                     /locus_tag="ACHA6Y_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8986523.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            9176..10369
                     /locus_tag="ACHA6Y_000405"
     CDS             9176..10369
                     /locus_tag="ACHA6Y_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8986524.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            10574..11713
                     /locus_tag="ACHA6Y_000406"
     CDS             10574..11713
                     /locus_tag="ACHA6Y_000406"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8986525.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            11706..12506
                     /locus_tag="ACHA6Y_000407"
     CDS             11706..12506
                     /locus_tag="ACHA6Y_000407"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8986526.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            12506..13333
                     /locus_tag="ACHA6Y_000408"
     CDS             12506..13333
                     /locus_tag="ACHA6Y_000408"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8986527.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            13338..14045
                     /locus_tag="ACHA6Y_000409"
     CDS             13338..14045
                     /locus_tag="ACHA6Y_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8986528.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            14145..15011
                     /gene="rfbA"
                     /locus_tag="ACHA6Y_000410"
     CDS             14145..15011
                     /gene="rfbA"
                     /locus_tag="ACHA6Y_000410"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM8986529.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            15025..15597
                     /gene="rfbC"
                     /locus_tag="ACHA6Y_000411"
     CDS             15025..15597
                     /gene="rfbC"
                     /locus_tag="ACHA6Y_000411"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM8986530.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            15619..16647
                     /gene="rfbB"
                     /locus_tag="ACHA6Y_000412"
     CDS             15619..16647
                     /gene="rfbB"
                     /locus_tag="ACHA6Y_000412"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM8986531.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            16745..17596
                     /gene="rfbD"
                     /locus_tag="ACHA6Y_000413"
     CDS             16745..17596
                     /gene="rfbD"
                     /locus_tag="ACHA6Y_000413"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM8986532.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            17630..19663
                     /locus_tag="ACHA6Y_000414"
     CDS             17630..19663
                     /locus_tag="ACHA6Y_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986533.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            19707..20987
                     /locus_tag="ACHA6Y_000415"
     CDS             19707..20987
                     /locus_tag="ACHA6Y_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM8986534.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            21197..22003
                     /locus_tag="ACHA6Y_000416"
     CDS             21197..22003
                     /locus_tag="ACHA6Y_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8986535.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            22015..23235
                     /locus_tag="ACHA6Y_000417"
     CDS             22015..23235
                     /locus_tag="ACHA6Y_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8986536.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            23225..24811
                     /locus_tag="ACHA6Y_000418"
     CDS             23225..24811
                     /locus_tag="ACHA6Y_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8986537.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            24850..26988
                     /locus_tag="ACHA6Y_000419"
     CDS             24850..26988
                     /locus_tag="ACHA6Y_000419"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8986538.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(27356..28339)
                     /locus_tag="ACHA6Y_000420"
     CDS             complement(27356..28339)
                     /locus_tag="ACHA6Y_000420"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8986539.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         9245 a         4972 c         5713 g         8758 t
ORIGIN      
        1 gcttgtgaag aaaatcaaaa actaattttg catatcaaaa aaaacatgtt acaatgaaat
       61 taatcaatgg tggaagcgtt ataaaaaaat taaggagatt aaaaaatatg gcaagaaaaa
      121 agacgattac tagagaacag attttggcag ctgcttatga agtagctgcc accgatggat
      181 ttacacgatt tactgcgaga aacatcgcga acaaaatgaa ttgttccacg caaccgattt
      241 atcttgagtt taaaaatatg gatgacttga gacaagctct ttttgagcag atctatgatc
      301 atttacaata tgaagtgttc actgttgagc atacagggaa tacgattatc gatcttgctt
      361 ttaattatat ccattttgca agaagagaga agaagttgta tcgttcactg tacttagaag
      421 aatatggtgg aggaaaagag atgcagaatt tctcttatac ttactttgtg aatacagtga
      481 aacgcgatcc agcttatgtg aatctttcag aagagcagat cgaatctttg tataatggga
      541 catggatcat agctactggg atcgcagctt tgatgtcttc aagcatcatc catccaactg
      601 atgaacagat tgaaaagatg atccaagaca gtatagatgc tatcttgaaa ttcaaagaac
      661 caatcgaaat cgattgaata tactcaaaag ttccacaaat cagtaggtaa caagagatgt
      721 gtcaaaataa ggaaaagttc gtttttctta cagcgtatgc atttttaact cccgaagaac
      781 tcttccatga atgcaatgaa taaaagagcc atacagattg aataaatctg gatggctctt
      841 ttattgcaag tatattaaat ttttgcgact agttctttag cgaaagcttc taaattttcg
      901 atatcttctt cttcagcatt cagatccact ttaacatttt cggctccttt tgatgcgcct
      961 gtttttgcaa aagcagcatc gaaatcatcc accgatttgc agaattcatc gtaaaaagta
     1021 tcaccagatc cgcagacacc gtaaattttt cccaatagat ccaattcttg caagtcttca
     1081 taaaagtcta cgatctcatc aggcagatcg ccatcatcgt atgtataagt agctactacg
     1141 caaatatctg cctcctcgaa ttcatctgca tctacttggg tacattcgtt gatttcgact
     1201 tcgatatcca gattttcaaa tgcttctgca acaatatcgg cgatttcttc agtgtttcct
     1261 gtcatgctag catatacgat ttttgctaaa gccatgatgt gcctcctcgt ttataattat
     1321 ttaacaatta tgaattatac caaaacatca ttgaaaagaa aataaaacct agagacacct
     1381 ggggaatgtc atttggctaa atgtatgccg ctatgttaaa atgagaggga tttgtaaaaa
     1441 ggagacgaaa atatgattac tttaaaatca gcacgtgaaa ttgaaatgat ggcagaatct
     1501 ggagctttat tagcagatgt acataaaaat ctgcgtgatt ttataaaacc aggaatcaca
     1561 agttgggata ttgaggtatt tgtcagaaac tatatcgaga gtcatggcgg aattgctgcg
     1621 caaattggct ttgaagggta tgaatatgcg acttgtatca gtataaatga tgaaatttgt
     1681 catggattcc ctcgtaaaaa gcctttgaaa aacggtgatt tggtaaaagt cgatatgtgt
     1741 atcgatttga aaggcggcat ttctgattct tgttgggcct atgtagttgg agaatctaca
     1801 cctgagatcg accgattgat ggaagtaacg aagaaagctt tgtacatcgg tattgagcaa
     1861 gcacaagtag ggaatcggat tggtgacatc ggtcatgcta tccaaactta tgtagaaggc
     1921 gaaaatctag cgatcgttcg tgattttatt ggtcatggcg ttggtcctac gattcatgaa
     1981 gaaccagcag ttcctcatta cggagaagct ggaaaagggt tgcggttgaa agaaggcatg
     2041 gttatcacga tcgaacctat ggttaatacc ggaacttgga aaatgaaaat ggatcctaac
     2101 ggctggactg cgtatacaag agacggcgga ctttcttgcc aatatgaaca tacattggct
     2161 attactaaag atggtccaag aattttgaca tcacaaggag aagaaggcac ttattgatct
     2221 atccagtcaa taggtacggg agaggtacat gaaagtttgg aacaaaataa aacaaaatga
     2281 aaatctgatg cgctttattg agacgactca aaagcgcatc actgattcag aaatgagtac
     2341 gacctctgtc gtcgttgctt attatttact cttgtcttta tttccattga ttattgcttt
     2401 tggtaatatt ttaccgtttc tccacatcga tcaagagact gtactgacct atttacgaga
     2461 agtgattcct gagaccgttt accagtttat cggtcctgca atcaaagacc ttttgacaca
     2521 aagttcagga ggcttgttat ccgtttctgc attagcggct ttatggtctg caagtcaaag
     2581 tatcaatgct ttgcaaattg cgatgaataa agcgtatggc gtagaaaata gaaaaaattt
     2641 tttgatcgtc cgtttcttct cactaattgt cattgtcttg ttcatgattg ctattagtgg
     2701 ggtaactatc gttcttgggt tgggacagct gattttggat gctattcagc caatcgtaca
     2761 aatacccgtg aactttatcg atcagttcca aactttgaag tggccaatca cattggtagc
     2821 tttatttgtc atcatgttct tgatttattt aattgtccct aatgctcagt tgaagttgaa
     2881 gacgatcatt ccgggcacga tcttcgctac tgtcggctgg atgctgttat cacaagtgtt
     2941 tagtatctat gctaggtatt ttgcaacgag gatcagcggt tatcagatca taggaagttt
     3001 tattgtactc atgctttggc ttaattttgc tgctacgatc atcattttgg gcggaatcat
     3061 caatgcagtt gttcaagaat acgtgacggg tcatcaaatc aaaaaacgtc gagatccaag
     3121 ccgaaaatgg ttcaataaat tgaaaactaa attttctcat aaaaaaaagt agaaacaaga
     3181 gttcttattg taaatttata tcagtaattt atttgaaatt ttatatctaa ttttcaatta
     3241 tccgcataaa cactatacat tcaagcattt tgaagtatag aaaaacactc atcttaccac
     3301 gaatttacca cgattgaatg agccttgata tgaccgtaaa actactaagg gagatagcac
     3361 gaaactgtgt atctcccgat ttttttatac agttgattac ttcactccga tttcaaattt
     3421 caatattgct ttcatcatgt ctgtatgaat ttctcctttt agttcttcat caacctgcat
     3481 aatgatttta ccactttcag agtggtaaaa aggacgcaag cataatttag aaatataacc
     3541 gtcataaaac ttcaaaatct ctttaactgc catttcatct ccgcttgacg ctttgcaaat
     3601 caattcaaaa gagggatagc cataatcttt actcatttct ttacttcctt tcgctcaaaa
     3661 gcatttttaa cttccttagt cctctagttc ttctactgca aatagcagaa cgtgatacat
     3721 ggtacagttc tgaaatttct ctgtcgttca ttccttgaaa ataacgtaat aaaatcgcac
     3781 tacgctgttt ttctgacagt ctatgtaaaa ctgtaccaag tgtttcatca gatacataaa
     3841 ttgtaaagtg caagacttta aatgatacac aatcataaaa acaggtatca atcgttccac
     3901 gttcaacttg tttcgtttcg gctatttcac aaaacaatac ttcacgttta gaatgtcttg
     3961 caagctgtct gtttcgactt ttcactttgt tcttaatgac aatcagcatt aaagaattga
     4021 actgtaatct gacgatatgc tcgaaagaag atgtcctcat aatctcaccc cctttctgct
     4081 aatgctgaaa tatataaacg gaataccgtt tatacccttt agaaatacag aaaaatggta
     4141 agaaaaaagc ccacacaaaa catatatttg tgcgagcctt aaatactgca atattactct
     4201 ttcttttata acactatcct tttagcagat ttcaaatttt cttttatata ttgtttttag
     4261 cctaacatac tatttaaccc cttgtattat atctctaact tttgaaaaat tctttttgaa
     4321 tatattgaaa tcatgattga agcaatagaa gctacaaaca taacaaccca tgtttcttgt
     4381 ataaatacat cgaataaaat accgtaaacc atttgtccaa taggttgacc gcacatagca
     4441 actgctacca gcgtagccat tatctttcct actaattgcg gtggtgtttg tgtctgaaca
     4501 agagtgtaaa tttgaacaac aaacatagta gatacaccca tagccacaaa gcacatcact
     4561 gtaactaccc agtaggaaac tattgcaggc aattctaaaa atagcgaaat tcccataagt
     4621 aaaacagaaa cagaacaaat aagtaacaag gtataaaggt tttttagttt taactttgaa
     4681 gcaacaagag ctgttaaaat accaccacat aaaccaccta atgataatgc tgattgtgaa
     4741 aaaccatata gtgtatctgg catttttaaa acttcagaaa ttaagatagg cagaccaaca
     4801 attaaaacag cacttaaaac caaattgaaa atgcaaacca aaaagattac ggatataaaa
     4861 atgggtttct cctttttcaa aaaggtataa ctatctttta aatcgttttt agcaactgca
     4921 attacaccta cttcacgagg tcttttttgg tgtggaatat ggataaaaat ttccataatg
     4981 gctgaaaaag taaaacatat aatactaaca attaaaatgg gattgatacc ataaaattga
     5041 aaaactactc cgcttatagc tggtccgata aagtttgcta aagtatttac ttgatttatt
     5101 atagcattac cgacggtaag tttatctgat gataccaata gaggaatact tgcttgtacc
     5161 gctggttgat atgcaccaga aataccataa agtaacatca aagcaacaat aaacaaaggt
     5221 atgataggaa tttttcccaa tgacaaataa aatactaaaa ttatgattgc tgttacaaaa
     5281 tctagtatga ccataatgtt tcttttattt gtcctgtcag caataatacc acctaccagc
     5341 gacaaaataa ccataggtaa aaaagagcag gctgtagcaa taccaaacaa agctgatgaa
     5401 ccagtttctt ttaacaaata gtacggtaat gcaaatcgta ggatagcgtt tccaaataaa
     5461 gaaattatct gtcctataac aacaagtata aaatcacgat taaaaagttt tttttctgct
     5521 tgcataaaat ctctcctttt tatttaatca aacagaccgt cggtttatat aatggtttaa
     5581 ataaactgct cttttataga gcagtctatc gtatagggat aacaaataca gttatttaca
     5641 aaactgtata aatgcagaaa ttgtttcttc gtctaataat gtgtctatgc ctattccatt
     5701 taaaagggca ttgaatgtat tgcaacgttt ttccataccc tctttatccg tcatttcaac
     5761 taatgggttg agccatacat tacttaatac catgataact tctgcaattt catgtgaatt
     5821 ttcaatctcc aaagacttat ccttgatacc ttgttctaaa acgggttcaa caaattcagg
     5881 tactacaatt tcatacattt gttctatttg catagcaaga aaacgaggat tgtttaatag
     5941 ttgaggagaa actgtcaata aaacgctttg gttagaattg aataccgctg tcttaaatat
     6001 ttctcttaac ttttctaaac cgtttaaact atcgttatct ctaattttag caaatattac
     6061 tgcattttca tttccaattc tttgaaaaat tgcttttaaa atatcttcct ttgaatcgaa
     6121 atgatgataa atagcacctt tagaaagttt tgtttcattg ataatatctt gcaatgatgt
     6181 gttatcatat cctttttcac aaaacatttt tgtagaaaca tctaaaatga gttttactgt
     6241 ttcttctgga tatttatttc gtgccataac ttacctccaa tacaaaccgt tggtctgatt
     6301 aaagtatatc ttagtatttt atgcttgtca atataaagac ttgcaatttt ataaaaatgc
     6361 agaatgcaag cctttttcat ttattctaca atcttccagt tgctttcctt atgcggtgtc
     6421 aattcatact gtgatacctg cgttgccttt gcctgattta ccacaaatca gattttatcc
     6481 ctttcttcct cttgattgcc cacaacagaa aagaaaaagc cctgtcaaga gctttgccac
     6541 cattggtggt gctttgcact cttgatcggg ctttttgttt gctgtatctt ctcgcaagag
     6601 gaaaaaagtt actcgtcctc atcatcaaaa ttgttattcc ttaatacttc tcgtaacagt
     6661 tccatatctt cttttgagtt cgggtttatc attttcacga cttgataagg tgtcttatac
     6721 ttatgtccct ctatgctttc cccaaacata tccatactgt ataaatcatc atagcggtct
     6781 aataattttt gaaacttcaa atgctggata aattctttga ttgggtaaag gtcggacgct
     6841 tcaatgcttt tcccgttgat atagtctgta atatattccc gtaacttttc taactcatat
     6901 tccagccatt cttcctcgct gtcaaagaag ttatcatagt gtccatgttt cagttctgca
     6961 tttagacgtt cttcggctct taaaaactga cagacttcct tttcagattg attgacatac
     7021 ttccattctc ccgacaacaa ttcattgggc gtaacgtcca gcacttccat tatcttttcc
     7081 agcgtttcat aggcaggata gttcaaccct ctttcaatct tggaaaggct ctgcatattg
     7141 ataccgattt tgtccgcaag ttcctgttgt ttcattcccc taaattttct tatggtctgt
     7201 atattatttc ctaaatggct tattttcatt gcataaccct ccatatctcg ttatatcttc
     7261 gctattataa gtctatcatg cttaatatga tttgtaaagg caaaaaaaga tattgacaaa
     7321 taagcatata agaattacta tgttcttgta atcgaataag cacaatagaa tgaaatataa
     7381 aaacattcaa aaagaaatac ctatctgatt aaatccgtag atatgaatat cggttgaaat
     7441 ggaacttgtt agaaaaggca aaacagaact tgctgacaaa gtatttctga cacggtggcg
     7501 gaagcctctc aggcttaccg ccatgacacc ttgtgtcaag gggaagaaat gctttgtcgg
     7561 ctggcgagcc tgcgagcctt gtctgtgccc ccgttatcta acaggggggc actatttcac
     7621 aaaagacaag tcaaaaacca tacaccccca aacctgtgag tagtaatgag aggttttaag
     7681 aagatagtac atatcacact ttttgaaagg tggtttacag gtatttcaag gctattgtgg
     7741 gattggagga atacaatgaa caatttagaa tttgcacttg aaatgaagaa caaacgtctt
     7801 gccagcggtc tttcacaagg agaacttgca agtcttaccc atgtatcaag atatagtatc
     7861 aaccgctttg agaacggaaa agctaacgcc agcaaggaaa cacagaacat tatcctgcgt
     7921 tgtctgaatt actatatctg cgataagcct ttttatctgc tcgttgatta tctgtctgtc
     7981 cgttttccaa cgactgacgc attggaagtt atccgaaagg tgcttggcat gaaagcagat
     8041 tattttatcc attatgacta tgggtattat ggctataagg agcattatgc ttacggggaa
     8101 atcaaggtca tggcttctga tgatgaacac atgggcgtat ttttagaatt aaaaggggca
     8161 ggctcacgca acatggaata tgtcttacag gcacagaaca gggactggta ttcattctta
     8221 aaccgctgtc ttgactgtgg cggtgttatc cgacgttttg acttggcaat caatgatatg
     8281 tgcggtctgc tggatattcc tgtgctatct gaaaaataca gaaatggtgg tgcggaatgt
     8341 cgctgtaaaa actatgaaaa tgtacaaggt ggaaaattaa gcggaaaaaa ccgtaattta
     8401 gcaagtaccc tttatatcgg ctcaaaggca agcacaaaat atttctgcct gtatgaaaaa
     8461 caaaaggaac aggcaacgaa aaagaaacat accgatatta tcaaccgttt tgaaattcgt
     8521 ctgcgtgaca aaaaggcggt acaggcagtt gaagaactgt tattgacgta taaccctcat
     8581 gggctggtgt tttatctcat taccgatttt gtggaatttc ctgactatcc attgtgggag
     8641 atattcattt cccatgacag tttacctttt gaaatgaacc ctgtacctgt caatatggaa
     8701 cgcactctgc aatggctgga aagacaggtc atgccgtcaa tcgtgatgat agaggaaatc
     8761 gacaggctga caggttcaaa ctacatgaaa atgattgatg aatgtaccca cctttctgaa
     8821 aaacaggaaa tgcttgtgga acagatgtgt acggacatat cagacgtaat cgaaagtgag
     8881 ggggtgtttt atgagtaata cacaggatat tcccgtatgg gaaaaataca cccttaccat
     8941 tgaagaagcg tcaaagtatt tccgcattgg agaaaacaag ttaagacgat tggcagagga
     9001 aaacaaggac gctggctggc tcattatgaa tggcaaccgc atacagatta aacgccgaca
     9061 gtttgaaaag gttattgata aattggacgc aatctaatgc aaatgagcct tgtatgtgtt
     9121 atgatgaaca caagtcatat caaggctctt tccaacaagg aaaggagcag acaccatgaa
     9181 agaaaaaaga cgggataaca aaggtcgtat cctgcatact ggagagagcc aacgaacaga
     9241 cggaaaatac ttatataaat atgtagacgc attgggaaac acaaaatatg tgtatgcttg
     9301 gagattgaca cccacagacc cgacaccaaa gggaaaacgg gaaaaaccct cacttcgaga
     9361 actggaacag cagataagac gggatattga ggacggtatc gacagcacag gcaagaaaat
     9421 gacgctttgc caactctatg ccaaacagaa cgcacagagg acaaatgtga agaaaagcac
     9481 acagaaacaa cgggaacaac tcatgcggtt attaaaagag gacaagttag gtgctaggag
     9541 cattgatacg ataaaaccct ctgacgctaa agaatgggcg ttacgcatga aagacaaagg
     9601 cttttcctac aataccatta acaaccataa acgctcgtta aaagcgtcat tctacatagc
     9661 catacaagat gattatgtga ggaaaaaccc ttttgatttc aagctaagtg aagtcataga
     9721 aaatgatacc aaagagaaag tcgcattgac agaggaacag gagcaagcct tattgtcatt
     9781 tattaagacg gacaacgtgt atcacaagca ttatgatgat gtgctgatac tgttaaagac
     9841 aggacttcgt atctcggaac tgtgcggact gacagtagcc gacattgatt tcaagaatga
     9901 agttgtgatt atcgaccacc agttactaaa gagtaaggaa cagggctatt atattgaaac
     9961 gcctaagacg aaaagcggga taaggcaagt gccattaagc aaagaaacga tacaggcatt
    10021 tcaacgggtt atcaacagcc gaccaaagat aaagtcgata gaggtagacg gacacagcgg
    10081 ttttgtgttt atcaatccga aaggtaatcc aaaagttgcc attgattata acgccttatt
    10141 tatccgtatg gtaaagaaat acaacaagca ccacaaggac aatcccttgc cacatattac
    10201 accgcatacg ctacgccata cattctgcac aaggctggca agcaagaaca tgaacccgaa
    10261 agatttacag tatatcatgg ggcattcaaa catcagtatc acaatgaact ggtacgctca
    10321 tgcgtccata gataccgcaa aatcagaggt tcagcgtcta atcgcataga agtatttacc
    10381 acgattttaa ccacgattga cagggaaaat ataagaagat agacctagat atgcgaggtt
    10441 taccacaaaa gcaaaatgcc cgtagagccg ataaaataag gctttgcgga catttgagaa
    10501 gatataaaaa gatagtcaaa aagacatata taatttataa taattatgac tcttaattaa
    10561 agtaggtgcc catatgcttg tttcttttag tatggttata agattttttc tgacaatttt
    10621 attatcactg atattaacac ctgtattcaa aatcatttca gtacaaacag gtatggtcga
    10681 caaaccaaat gaaagaagaa taaataaagt tccaatgcct tcagcgggcg gactgcctat
    10741 ctttatttca tttgttatat cgactttatt cttattccgt aaaattatcc ctcaatcgta
    10801 tattgtaccg atcttgattg cttctttagt aatcatagtc acaggagtgc tcgatgataa
    10861 atatgagctg acacctaaac aaaaaagtat agggatcttg attgcttctc ttattattta
    10921 ttttgtagca gatatacgta ttgattcctt cacgcttcct tttattggat atatccaact
    10981 aggctggctt agttttcccg taacgatttt ttggatcttc ggtatcacga atgcggtcaa
    11041 tctgattgac ggtttggatg ggctggcggc agggatttca ttgatcggtt taattacgat
    11101 aggaatcatt ggatattttt tcctccatgc ttctacagtg tatattccag ttgttatttt
    11161 ctgtcttgta gcaagtatta tcggtttttt tccttacaat ttttatccgg caaagattta
    11221 tttaggcgat acaggagctc tattcctagg gtttatgatg gccgtccttt cactacaagg
    11281 gctgaagaac gtgacttttg tttcatcgat ttctttgctg atcgtcatgg gagtgccggt
    11341 aacagacacg ttttttgcta tcattaggag aaaagcaaac cgtgtttctt tttctacagc
    11401 agataaaaaa catcttcatc accgactaat cagtttaggc tttactcata aaggtgctgt
    11461 cctgacgatt tatgcgatgg ctttgatgtt ttctttcaca gctatggtca tgaattatac
    11521 tggaagaatc ggaacgatca tattgattat tgccatgttg tttgctgcaa ttttattatt
    11581 tgaattgatt ggtctgatca atgagaaaca tcagtttatt ttaaaaacat tgcgattttt
    11641 agggaataaa gagtaccgta cgcaagtaaa acagaaatat ttgaagaaat ggaaccgtca
    11701 tatagatgaa taacaaatta aaccagccga tccttattag tattgtgacg gcaaacagtg
    11761 ataaaatttt tcggacacta gataatttta tttcaactgt aaaagatgaa acagcagtaa
    11821 aaatccgaat ttttgacaac aattcacttc ccgagtacaa aaaaagacta acagagtata
    11881 ggaaatatcc ctacatcgat atctattttc atgatgaaaa ttccggattt ggttatggac
    11941 ataatcacaa tctgatagct agcgattctc gctatggcat tatttgtaac ccagatattt
    12001 tagtggatga agagacagtt acagcattgg tcttgttatt gaaagaacat ccggaatgtg
    12061 cgatgttagc acctaagatt cttaatgaag atggaacaac tcagcacctc atccgtgagc
    12121 agctggctgt ttttgattac gttttgcgct ttgtgccttt caaatgggtc aaaaaaaagt
    12181 ttgacaaacg actggctgtg tttgaatgcc gaggattgcc tactgatgct aatagttatg
    12241 tacggatgat ctcaggtagt tttatggtag ctgatattga taaatttaaa gagataaagg
    12301 ggtttgatga gcgtttcttc atgtactttg aagacaacga cttgtgtttg agttttgaaa
    12361 aagcaggtta caagttattg tacacaccct tttatgcagt tatccatttt tacgggaagg
    12421 gtgcgcatcg aaatgcaaaa ctgtttcgcg tgtttttgac ttctatgatg aaatttttca
    12481 ataagtgggg gtggcgtttt ttctgatgaa accagaaata ttagtagtcg ttgtgcttta
    12541 tcagcaacta ttcagtcaat ctccagctta tggaccatta agcaaagcac ttagcgaaaa
    12601 gcaaattcag cttattattt acgataatag tcctcaaagg cagcaaaatg agctgtttga
    12661 aaaagaaaat atcgtttatc atcatgatcc gcaaaatcct ggtttagcgg tggcttataa
    12721 ttttgcgttg tctcaagcag atgaaggaac acgttgtctc gtcactctgg atcaggatac
    12781 gaggctagtc gacgattatt ttgaactatt gcgtaaagta acctttacag atgaatgtgt
    12841 tgctgcagtt ccgatgattt tttctgggag ccgacaagtt tctcccctat attcagatcg
    12901 ttatatcaat cgtacagctc aagcagtaga agttgaaggc ccaacagctg aacgaatcat
    12961 ggcaatcaat tcaggtgttg cctggtctgt caaatttcta aaaaagattg gcgggtttaa
    13021 tcctgctttt tctttagatt ttttggatca ttggctgttt tggaaagtgg atcagttgga
    13081 aaaaactgtg gagatccttc ctgtacgaat ggaacatgat ctttcggttt tagactatga
    13141 aaaagtttca agtaatcgat accgttctat tttacaagca gaaaatcgat tttatcatga
    13201 atatgatcaa aggcatttat ccagtcaccg acgacaatta ttgttaagaa cagctaaaca
    13261 gttcttgacc gtcaaaaacc ggcatatctg gcggatgact ttgcgctcat atgttaagaa
    13321 ttggaaggtg tgaattcgtg cgttcggttt gtatagctac ctttaatgga ggcagatatt
    13381 taagagagca gttagatagt attttatctc agctttcagc agaagatgaa gtagtcattt
    13441 ctgatgatgg gtcagtagat gatacatggg atatattaac tgaatacgca gaaaaagata
    13501 agcgaatcca gcttttttcg gggcctcgtc aaggattgat cgctaatttt ggttatgcga
    13561 tccagcagac gaaaggagat ctaatctttt tggcagacca agatgatgtt tggttggaga
    13621 caaaagtgaa aaagatcact gactattttg agcagaatcc ggatcagcta gtagtagttt
    13681 ctgatttaat cattgtggac agcgagctgc aagagatcca tgattcgtat ttttcgtttc
    13741 gaaattccaa agaaggatgg cttaataatg tgataagaag tcactatatt ggtgctggta
    13801 tggctttccg gtcttcttta aaacccgata ttttgccgat cccgtcagaa gtgccgatgc
    13861 atgatatgtg gattggtttg ctgggaggaa agaagactgg gttcctaaga gaaccgttaa
    13921 ctctgtaccg aagacatgat ttaaacgcga gtgagattga aacacattct aaattacggc
    13981 aaaaaatttc ttggcgtgtt catctgatta cggcgttgat caaacgaaaa tggtttaaac
    14041 gttgataaaa gatgtttaaa tccttaatat ttccatgaca aatataacaa ttgatgagat
    14101 aataacaaag tgaaatttga aatcaaaaag gagagcagat atccatgaag ggaatcattc
    14161 ttgctggggg cagcggaaca cgtctgtacc ctttaacaaa ggcaacatcg aaacaattga
    14221 tgccgattta tgacaaacca atgatttatt atccaatgtc tactctgatg ttggcgggaa
    14281 tcaaagaaat tctgattatt tcaacgccac aagatacacc acgtttcaag gaattatttg
    14341 gaaacggaaa tgatttagga ctgcagatcg aatatgcagt acaagaaagt ccagatggat
    14401 tggcacaagc gtttatcatt ggagaagaat ttattggaga tgatagtgtc tgcttggtat
    14461 taggggacaa tatttactat ggtggcggtc tgtctaaaat gttgcaacga gcagcttcaa
    14521 aagatacagg cgcgacagta tttggctatc atgtcaatga tccagaacgc tttggtgtag
    14581 tagaatttga tgagaatatg caagctttgt cgattgaaga aaagcctgct cagccaaaat
    14641 caaattacgc tgtaactggc ctttattttt atgacaatga ggtaatttct atcgctaaag
    14701 gtatcaaacc ttcagaacgt ggagaactag agattacgga tgtcaataaa gcatatttgg
    14761 ataaaggaaa actatccgta gaacttatgg gacgcggatt tgcttggctt gatacaggaa
    14821 cacatgaatc attgcttgaa gcatctacct ttatcgaaac aattgaaaaa cgtcagaact
    14881 taaaagtggc ttgtttggaa gaaattgctt accgtatggg ctacatcgat aaggaacaat
    14941 tgctggccct tgcacagcca ttgaaaaaaa atcaatacgg tcaatattta ttgaatttgg
    15001 ccgctgaata aggagtcctt acagatgaaa gtaacagata caaaattaac agatgtcaaa
    15061 atcgtagaaa tggacgtctt tggcgatcat cgcggtttct tcacagaaag ttattcaaaa
    15121 gcaaaatttg cagaacatgg attgaaatat gactttgttc aagacaacca ttcactatca
    15181 gctgaagctg gtgtccttcg ggggcttcat ttccaaaaag gagaagctgc acagacaaaa
    15241 ttgattcggg tagtcactgg agcagtttta gatgtagtag tggatatccg caaaggtagc
    15301 ccgacttatg gtcaatggga cggatttatc ttatctgaac ataatcatcg acagttgctt
    15361 gtacctaaag gatatgccca tggattcgtt actttgacac caaatgtgaa cttcctttat
    15421 aagtgtgata attactataa tgcagaagca gatgggggca tcgcatttga tgatccagat
    15481 ttagcgattg attggccaat tgatccatca agagcgatta tgtcggaaaa agatcaccgt
    15541 catccgacat taaaagaatt tgaagcagaa aatccatttg tttacggaga aatctaagaa
    15601 ttaaggagaa attttcttat gaaaaacatc attgttaccg gaggtgccgg attcatcggc
    15661 tctaatttcg ttcattacgt ggtaaacaat catccagaag tgcatgtcac tgttttggat
    15721 aaactgactt atgcaggaaa caaagagaac ttggctggac ttcctagcga tcgcgtagaa
    15781 ttggtcgtag gagatgttgc agatgcagaa ttagtggacc gtctagtaaa agaagcggac
    15841 gcagttgttc attacgcggc agaatcccat aacgataatt cattaaaaga tcctttccca
    15901 tttgtacaaa cgaatatcat tggtacgtat acacttttag aagcttgccg caagtatgat
    15961 gtacgctatc atcacgtatc tactgacgag gtttacggcg atcttccatt acgtgaagac
    16021 ctgccaggac atggggaagg cgaaggagaa aaattcactg cggaaacacc atataatcct
    16081 tcaagtccat attcttctac aaaagctggt tcagatctgt tagtaaaagc atgggtgcgt
    16141 tcttttggct tacgtgcaac catttcaaac tgttcaaaca actatggtcc ttatcagcat
    16201 attgaaaaat ttattcctcg ccaagtaacg aatatcttaa gcggtattcg tccaaaatta
    16261 tatggcgaag gaaagaatgt tcgtgactgg atccatacga atgatcattc ttcagcagtg
    16321 tggctgattt tgacgaaagg cagaattggc gaaacctatc tgatcggtgc agacggtgaa
    16381 gaagataata agaccgtgat ggaattgatc ttagaaatga tgggtcaacc aaaagatgcg
    16441 tacgaccatg taaacgaccg tgcaggacac gacttgcgtt atgcaatcga ttcgacaaaa
    16501 ttacgggaag agttaggatg gaagcctgag tttacgaact tccgtgatgg gttggctgac
    16561 acgatcaaat ggtacgaaga acatgaagac tggtggaaaa aagaaaaaga ggccgttgaa
    16621 gcagcatatg ccaaaaatgg acaataatac agagaaaatg tcactgtatt aatggaaaaa
    16681 cacatgtgag gcaggcgctc gcctgtctct tttttaaaat aaaaatcaag gagcggataa
    16741 tcatatgttt ttattaactg gaggaaatgg gcaactaggt acagaattgc gccatttatt
    16801 agatgaaaaa aatctgcaat atgtttcaac agatgcaaaa gacttggata tcacagatgc
    16861 cgagaaaaca atggcttaca tcacagaatt aaaacctgat gttattttcc attgtgcagc
    16921 ttacacagca gtagataaag cagaagaaga agcaaaagaa ttagatgaga agatcaatgt
    16981 tgatggtaca agaaatgtag cacttgcagc aaaagctgtg aatgctcaat tcgtctatat
    17041 cagtacagac tatgttttcg atggaaaaaa caaaaatgta gaatacgaag tagacgacca
    17101 aacgaatcca ctaaatgaat atggtcgtac gaaattatta ggcgaaaaag cagtagaaga
    17161 gattttagaa gactattata tcatacgaac ttcttgggtt ttcgggatct atggacacaa
    17221 tttcgtcttc acgatgcaac gcctagcgga aacccgcgac aaactgactg tagtaaatga
    17281 ccagtttggg cgaccaactt ggacacgtac tttggcagaa tttatggtat atgtcatcga
    17341 agaaaaagca ccatttggta tttatcattt gtcgaacgaa aatagctgtt cttggtatga
    17401 attcgcaaaa gaaatattga aagatacaga tgtagaagtt gcacctgtta cgtcagaaga
    17461 atttccgcaa aaagcaacac gtccgcagta ttcagtcatg agcctgaaaa agacagaaga
    17521 gttaggattt gtcataccaa cttggcaaga agcacttgcg caaatgctgg aagctgccaa
    17581 aaaaatgaat aagtaatttc cttttatcaa atagaatttg gagcatgata tggaaaaaca
    17641 aacaagaagt gcacgacgaa aagaaaagcc aaaagcgaac caggcagatt cgaatttggt
    17701 gcttgacctg tttctaaaac tccgctatct tattggcgtt ttagtcatcg tggtcatcgt
    17761 tacttttaat ttaaacggaa gttcacttgg tgtttgggat aaatatgtgt cgcaaagaga
    17821 cgatgggaaa aaaactgatg tgatttttgg acagaatcgg gaagtgcggt cagacgaatg
    17881 gctggtgcag acaccttttt acctctcaca agctgaagag gactttccgt tggtgaacga
    17941 ggattattca ttaaacggac aaaacatgat cattgcgtat aattctcctg taaaggatat
    18001 tacggttatc ggaaaaccat tcaactgggg attctttttc ctcggaagag atcgaggact
    18061 ttccttttat tgggcgatga aattagtcgt catggtcttg cttggattcg aagtactgat
    18121 gatattaacg aagagaaaaa aagctctatc gctaatcggt gcatttgcgc tgacattttc
    18181 tccagcagtc cagtggtggt tcatgcaaca tgtgggcgat ttgattttct ttactttggg
    18241 actgatgact gctttttatc attatttcta caatcatgat aaaaaatggc tacgaacgtt
    18301 gatgatgctt ttggtggtta ttttcggtat tgggtttatt ttagtgattt acccagccca
    18361 tcaagtgatg ctggcgtact tgctgatttt ttattttgtt ggcctgctta tttactatag
    18421 caagaaagtt tcatgggatc tgttggatgt agtgttgatt ggaggggcag ttttatttat
    18481 tggcggagtc atggttcatt tctggctaac gtcaaaagac gcattgttag cttctttgaa
    18541 taccttatat ccaggaaaac gggtaagtac tggaggaaag tggacgattg gcgaattttt
    18601 ttctttcctt acaaattgga aaattccttt tgaagatatc aactattcga ataactctga
    18661 agtggccctg ttctatcact tttttccaac tgtattccta gcaagtccat ttgtgttatt
    18721 tggcaaaaaa gatagcgaac aaaagttact gggacgtatt ttgatgattt tttgtctgtt
    18781 ttctatcttt tggattaccg ttgggttgcc aaaatggcta gcagaaatca ctctgctgtc
    18841 ttatgttcct ccattaagag ccatccttac ttttagtttt gcggcttgtt tattatcgat
    18901 ctggtttatc gcttatattt ggagagaaga tgttattcct atttggtata aattcatttt
    18961 gttggcagta aatgcatggt cttatttcta tgcgattacg cattcgaaaa tggataatta
    19021 tttttcggat tttgaaacca tcgcagttgc tgtgttggga acagtgattt tagcttttgc
    19081 attatttggc tggagacgct tgttttacct cgcttttgcc gccttagtta tcgtatctgg
    19141 ctttttcgtc aatccagtcg tagaaggtac aggagcgatt tttgaaaaaa cactggcaaa
    19201 agaaatccaa aaaatcgatc aacaagatcc tggtaaagtt tggttgtctg aagatgaatt
    19261 atataacttc accccaacat tgggagtgaa atccttcaat acagtacgct tttatccaga
    19321 tatagctgca tggaagaaaa tcgatccgaa agaagaatac gagaagtatt ataatcggta
    19381 tgctcataca agagctttta tcgctaagga agatacaaag ttcattttag atcgtccaga
    19441 tatgttttcg gtcacgttga attttgaaga cgcagaaaaa ttagggatcg actatgtgat
    19501 cagtaaacga ccattaactg attacaacca attatatgaa gcgcagttca cgcagctata
    19561 tggaccggat aaagacggtt atatgatttt taaagtagaa tatccggatt atccagatgt
    19621 accacaaaca caaactcagg aaaatcagct agtcgtacag taataggcta gctgagaata
    19681 atcctgaaat gttagtagaa aggagagtgg agataattca agcaatcgta caaaaatttg
    19741 tcaacaaaat gagtgtcggt ttcttgcttt tgttactgac gctagtcttt tttgtttgga
    19801 cagcagggtt aggttttcca gcaactttgt ttatatataa caatagttca ttacttatcg
    19861 cttttagtgt ggctattttg ctcattttaa atgtttataa agcaaaaggc gtcgattttc
    19921 tttatctagg tgttgcgctt gcccttttca ttttcttttc ttactttcgg gaattaagaa
    19981 atgagtcttt atttggagac ttactgtttc ctttgattat tttattttgt ttagtaatta
    20041 aatggatcga gtggacaaaa tgggatcgcg gtatttatct gattgtcttt tcggtcttcc
    20101 tcggaatcac gttatatcgt gtatttacag agatccaagt accagaagga caaagcatct
    20161 gggctccagg caattcgctt tctgatattt ggatcaatac gaatataatc ggaagttctt
    20221 tgatgactct tgctttattg atcagtggat ttgccagttc ttttgaacga tggtatgtac
    20281 gaatcatcgg tgcagtagca gttgttgcag cttttctcgg gatttgggtc tgccaatcta
    20341 gaggtgcatt agtcgcgttg attgtttttg cgatattgga tcttttacct aaatcgttga
    20401 tgcgtgtcat tcgtgcgcca tttattgctt acacggttac gattctttta gctttgccga
    20461 tttcttatct ggcggctgtc tctgaaaaag tcaatttgtt taccggtcga gaagatattt
    20521 ggcataaatt ctatcagacg ctaggagaaa aatctgaaca aattttacta ggaatgaaaa
    20581 catttatctt ccaacgagga aaccaatttt taggaaacca caatagttat aattctattt
    20641 tgaatatcta tggattgata ggatttggga ttgctgcttt gctgcttatc ctttttattg
    20701 gtcgcttaac gttgaaagca gatttatcca acgggcagat gacttttatc tgggcctttt
    20761 ttgcggtgat gatgcagtca tttatggagg atacattgac ttcataccca tgggttccga
    20821 ttatttatat cctgttggct atggccgttc atcgatatga tgaaccagaa gttgcggagg
    20881 aattctatgt tatggaagag gcatatgacg atatgactta tgatgataca tcttatgagg
    20941 aacaaccaga tagcgctcat tcttccagga tccaacgcta tcattgatag gcacaaaata
    21001 cgagagaggc tgtgacaacc gtcgcagcct tttttggttt attgcaattg tctagcatct
    21061 tctgtaaaat agaaacgtta gagaaaatct tgtatcatag aaaattaaga aagaaataag
    21121 aacgcagctc ggtaccaaaa atatggtaaa atggcacttg actgtaattc actgaaaatc
    21181 gcttggagga acacaaatgt ttaaagattt gtatttattt atgaaggaca tttatcagaa
    21241 cagaaaatta ttgctacagt tttcgatcaa tgacttcaaa tcaagatatg caggatcgtt
    21301 tttaggaatc ttatgggcgt ttatcaatcc gctatttact gtattgattt attggctggt
    21361 atttggtgtt ggactgaaag cacaaatgat cgatgcaaaa tacccattta tcgtatattt
    21421 agttaccggg atgattccct ggttcttctt ctcagatgca tttgcatcaa ctacggttgt
    21481 ttttcgggaa tatacttatt tagtaaagaa agtcgtattc aatattcgta tcttgcccac
    21541 gacgaaaatc ctatccaatc tgtacaccca cttattcttc attctgattg gcatagctgt
    21601 cgcgcttata aatggtatat atccttcagc aatgtcttta cagctgattt attatttatt
    21661 ttgcatgtgt gctttcttga caggattgac ttggctgacg gcatcgatcc aaccattttt
    21721 acctgatatc atgcaattaa ttacgatcgt gctgcagttg atcatgtgga cattaccgat
    21781 attatggagc cctaatcagt ttgattcagg tcttgtaagc atattgaaat taaatccgct
    21841 gtactatgtt gttcaaggtt atcgtgagtc atttttaagt gaagcttggt tctgggagca
    21901 tggaacatat actttgtatt tctgggtttt cacgttagta atgttcgtaa tcggctctgc
    21961 agtgttccgt agattgaaac cacatttttc agatgtgctg taaggagaat agctatgaca
    22021 gaatatgcaa ttagactaaa agatatcaca aaatcttata atatgtacgc aaaaccttcg
    22081 gatcgatttc gtgaagccct caatcctttt aagaaatcct atcatgatgt attttatgcg
    22141 ttgaaaaacg tgaatgtaga aataaaaaaa ggtgagatga tcggtttcgt gggagaaaac
    22201 ggatcgggaa aatctacaat gttgaaaatc attacgggag tactgacacc aacatccggc
    22261 acgatggaaa tcgaaggaaa aatctcagct ttgctagaac taggatcagg attcaatcct
    22321 gagtattctg gctatgaaaa tatctattta aatggaatgg ttctaggatt ttcaagagaa
    22381 gaagtcgatg aaatggtcga tgatatcatt gaatttgctg acatcggaga ccatatttat
    22441 caacctgtaa aaacttattc aagcgggatg ttcgttcgtc tagcttttgc tgtagcaatc
    22501 aatgtgaatc ccgatatttt gattgtggat gaagcattgg cagtaggcga tcttgaattt
    22561 caactaaaat gtatggaaaa atttacagaa atcaagaact cgggaaagac gattttattc
    22621 gtttctcatg atatcagttc gattcgtcgt ttttgtgatc gttcctattg gttgaaaaac
    22681 ggagaagtcg tcgaatatgg cgatacgatg gaagtaacga cgaattatga aaacttttta
    22741 aagaaaaaat cagttaaaac agtggatcgg aataaaaacc aagtagaacg gttcgccgct
    22801 ccagatatcg tcgacgtgac gaaagcggaa ttgttggata aagacaaaaa tccggtggat
    22861 atgctggaac aaggagaaaa actatacgtt aaagtaactt atgaagtaaa agatgataca
    22921 atcaaaaatc cggttttagg aattgcgcta cgaacagtcg acaattttta tgtatgcgga
    22981 cttaatactc tactagatgg tatcaagatt ccttggaaaa aaggagagaa tatcttttat
    23041 ctggagtatg aaaagttagg tttgttagct ggtgaatatt attttgacgt tgcggtcttt
    23101 gaagaaaatg caacggttcc tttggtatat aagacaaagt acatgaactt gtttgtcagc
    23161 ggttcttata tcggagaagg catcgtggtc ttagaccata aatgggaaga agggactcat
    23221 agcaatgaaa tatgattttg aaatggacct agatgaacaa agcagtgtgg gaaaaattgc
    23281 cgcacagatc aaaccaggaa gtaaagtttt agagtttggt cccggaaacg gccgattaac
    23341 gaaacattta atcggagcaa aacaatgtga agtgagcatt gttgagttag ataaggaatt
    23401 gttcgatttt gtcagcgaat ttgctcaaga tggattttat ggtgatatcg aaagttttga
    23461 gtgggcaaac tattatgctg gacagacatt tgattacgtc ttgtttgcgg atgttttaga
    23521 acatttggta gatcctggaa aaacattgaa gaaagtcaga gaatttttga atgaagaagg
    23581 agagatcctg attacattcc ctaacttggc gcataattct gtcatgatcg atttgttcaa
    23641 taaccaattg ccgtgggctt cttatggtct cctagatgaa acccataatt ctttttatac
    23701 tcatgatggc ttccaaaaag tttttgaaaa agcgggatta ttcattaata ttgaagatta
    23761 tttgtactta gctgtaggtg atacagaatt aaaatcaact tatgaagaat tgccggaagc
    23821 tgtacgctat gatttcaaaa tgcgaccatt tggtgaagtc taccaatact ttttctcatt
    23881 gatgaaacat ccagtagcac aaagttccat tgcagagcca caaaattcta attatgtaaa
    23941 agtgttagaa gtaacccagc aaacgaaaca agatgaaaca atccaacaaa taccattcaa
    24001 taatttcaca ggggaaaatg agacactaag tttccccgta tcagaaacaa cagaaagaat
    24061 ggtgtttcgc ttcgcgcaac aacctagctt tatcgaattc agcgcagaag ctgccggaga
    24121 aaaactaaca tttatcgatt cgaatgctgt agtcaaaaca gtaaatgatt gttatttgtt
    24181 tgatgggaaa gaattaccag agttcgttct aacggatatt tctgggaaag aagtgactat
    24241 ccattgtcac tatcgcttta tcggggaatt gacaccaaca atgaaagaat tgttggaaac
    24301 gattcgtccc atggctgaag tcacaaagca gctttctgag aaaaatgacg aattagaaag
    24361 agaaaatcat catcttcttg aggaaaatac aagactagac catacgttaa aaacaaccac
    24421 aaatcgctat tgcacattgt tggaaagtga tgaatggaca ataaaacata gactaggacg
    24481 acgtaagaaa gaaacttcga aaaaaatcca agaaaaagaa ctttctatat gtattgacga
    24541 gaaaatctgg gatgcagaaa caaaaatatt aaaaatcatc ggatggggaa tcgctaattc
    24601 ggatcgccag cccttgtcct ataagttatc agctgaccaa tcgccatttt tcgaagcaat
    24661 tcctgtttca cgagaagaag tcaatcaagc agaacagctc gcaaaaggaa cagaagcggg
    24721 atttgagtta cgaatccttt gcgagcaaga acgttcattc ttagtggaag cagtagctca
    24781 aaatggccaa tcatggatca ttgaaatgta aataatgatt aacttataaa gataaggagt
    24841 aaacgaatcg tgcaaaaaaa agaaattgcc gtatttatcg atcaaatcac tcgtgaacgg
    24901 gccactggcg atttgcttat tgtgggctgg gcaattgatg aagtgaccaa agaaatccct
    24961 acgataaagg tagaaaaaga aaatgtgatt gctgaagcaa cacatgtcgt aagattggat
    25021 atcaatcatt tatataatct tgatgttaag acccaaagcg gttttaaggt acgtttatca
    25081 gggaaaatga gaggaaaagc catcttagac ttccaaacag cgagacatca aaatggcata
    25141 gctgtgaaat taaacggtaa atacccttat gatgacggca ttgaatcttc atgggaaaga
    25201 aaaaaaagat tactgaaaaa agggataaat tatgcaagga ctcatggcgt aaaaaaagcg
    25261 atccgtcggg tcaaattgga attgaagcca ggatcgatcg actatgcaga gtggatcagt
    25321 cgtcatgaga aaccagattt gaaagagcaa aggaaagaag cacaaggatt tgcgtaccgt
    25381 ccattgattt ccatacttat gcctgtctat aacgttgaaa tcaaatggct cgaaaaatgc
    25441 attgattcgg tactggatca aacgtatgat cattgggaac tatgtatcag tgatgatgct
    25501 tctactgatc cagctatccg aaagtgtctg gaatcctatc aagcaaaaga tgatagaatc
    25561 aaagtcgttt tccgaaaaga aaatggacac atcagtctgg caactaattc tgcattggaa
    25621 atagcagaag gagaatttat agcactctta gataatgatg atgaattacc gccatttgca
    25681 ttatatgaag tggcaaaagt gctaaacgta catccagaac tggatgtgat ctacagtgac
    25741 gaagataaaa tcgatgcaga tggcaatcgc tttgatccgc atttcaaagc agactggtcg
    25801 cctgatacat tgatgggaaa taattatatc tctcacttag gcgtatatcg aacaagcatt
    25861 gttaaagaac ttggcggttt ccgtaaaggc tatgaaggtt ctcaagatta tgatttggtc
    25921 cttcgtgtga cagaacaaat ccctgcagat catatttacc acattgaccg agtcttgtat
    25981 cattggcgga cgatcccagg atcaactgca agtaatggcg aagccaaaag ttatatctat
    26041 gattccggag tgaaagcatt gacagatgcc ctgtctagaa gaaacatcaa aggaagcgta
    26101 cgtcctggca gaatttctgg attttatgag attgcttatg acgtcttgca agaggatttg
    26161 gtcagtgtga tcattccaac gaagaatggc tatgaagatc taaaaacttg tgtggattcg
    26221 atcatcgaaa aaacgtctta ttcaaattat gaaatcatta ttgcggataa cggaagtaca
    26281 gatccaaaaa tgcaagaatt atttgcagaa tacaagcatc agctgaaaga ccgctttatc
    26341 gtagaattga ttgacattcc attcaattat tctcgaatta acaatttagc agcagaaaaa
    26401 gcaaacggca aatacttttt attcttgaat aacgatacag aagtgattga gccagattgg
    26461 atgacggtga tggtttctta tgcacagttt gatcgaatcg gctgtgtagg agcaaaactg
    26521 ttttatccag atgatacaac gcagcatgca ggagtattat taggaatcgg tggtgttgca
    26581 gggcatgcat tgaataacta tgatcgaaca cattgtggtt atttcggccg attggtcatt
    26641 gacgtcaatt acttagcagt cactgcagct tgtatgatgg tcaaggcagc tgacttcaat
    26701 gctgttaacg ggtttgatga aacattagaa gtagcattca acgatgttga cttgtgcttg
    26761 aaagtttatg aactgggtcg ttacaatgtc tatgcacatc aagcagaatt gtaccatttc
    26821 gaatctaaat caagaggtta tgaagatact ccagaaaaac aaagacgttt tgctggtgaa
    26881 atcaaaaaaa tgcaagataa atggccagca tatattgcgc atgatccatt ttacaatgat
    26941 aatttaacaa aagaaggaat cggcgacttt tcgttgagac cagattaaac tataataaaa
    27001 agctgttatg ttgtgattcg ctcgcacata acggcttttt atagtttttt gtcaaaaaaa
    27061 ggcagaaggt gccgactcaa aaaaataagt cgaggttggg acagaagtgt ttaactccga
    27121 gaaataagaa gaaattcacg aaaattgctt ttcaaatttt tgtgaatttc ggcttattta
    27181 agaagaagtt gcttctgctc ccaccgttta ttcgttttta aagaatgaga caaaagtgtt
    27241 tcttactttt gtcccacgct cttattcatg ctcagagcgc caatagatac aaaatcatag
    27301 ccaaagtttc acgaacccta gctgtcaaag aatcgatatt ttattttaat ataggctatt
    27361 ttaaaataaa tggtttaagg tattcctcta caaaagccac tctatcaatt atcatgggtt
    27421 taaaataaga agtgatggag ataaccagat tgttcttagc gtcaatataa agaacatttc
    27481 cactattacc aattgctgaa tatatttcgt tctcagaatc taatacccac cataaatagc
    27541 catatctcat attttgtaag tcttctaaag cactggttct aggtttgaac atctcgttta
    27601 tccacatggg tggtacgatt tgttttcctt tatacatccc tttatttagg cataatacac
    27661 caatttttgc cagatcactt gccgataaac acaaaccata tcctgccgtc ccgatattta
    27721 gtggatcaca aaaccagaca ttggttttag gagacttatc cattgtaaac tgcttatgtt
    27781 cttcagctgt ttcagcataa taattgacat ggtttggtat ccctatagga tcaaatagat
    27841 acttattggc aaaatcaaca gttgttagct tacttgtctg atacaacaag ccagttaata
    27901 tgtgtaaaca gacagtttga tagttgaatt cgccggttat accttttctt cctcccaata
    27961 aatctagtga tgtttttgtc caatcttcac ttgaacagac ttttgtccat ggatcgcctt
    28021 tacatttgta gggtgctttc attgttagca agtgcttgat tgtaatagcg taaatagttt
    28081 tctccccgcg tttcacttta tactctggaa aataatctaa tactttatca tcgatacttt
    28141 tgattaatcc ttgctctaaa gcaatgccaa tcagcaaaga tacaatactt ttcgttgctg
    28201 acatgatatg acaagtatcg tcttttttat aaccattcca ttcatctgta taaacttcta
    28261 ccccatcctt atatgcgaca atttgacata tatttggttg tcgaaaacca ataaaatcat
    28321 gcaactcttg tttattcata agatattacc tcactttctt ctataagcat aatgtcatta
    28381 aaaatcgatt tcaatttttt cgattcttca cgtaatacag gtattcttat ggactagaca
    28441 gcgctttggg aagatggatt ctctgaaaaa tataaaggtt cactttatga gtggataaaa
    28501 aaagcagcaa atacttcgac ttctttaatt taccaacatt agtgaaaatc gattcatctg
    28561 gagatttgta atttagagtc cttgtcttga ttagaggtgt ataaagcttt ttattagaca
    28621 tttttaaatt ccattacaat ttgcttataa actaggtttt taaaggtgac tatggtaaga
    28681 taatagag
//
LOCUS       DAYYHX010000013        27775 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000013 DAYYHX010000000
VERSION     DAYYHX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.013"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(250..1194)
                     /locus_tag="ACHA6Y_000421"
     CDS             complement(250..1194)
                     /locus_tag="ACHA6Y_000421"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8986540.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1490..2434)
                     /locus_tag="ACHA6Y_000422"
     CDS             complement(1490..2434)
                     /locus_tag="ACHA6Y_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8986541.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2655..3869
                     /locus_tag="ACHA6Y_000423"
     CDS             2655..3869
                     /locus_tag="ACHA6Y_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8986542.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3891..5906
                     /locus_tag="ACHA6Y_000424"
     CDS             3891..5906
                     /locus_tag="ACHA6Y_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM8986543.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5989..7362)
                     /locus_tag="ACHA6Y_000425"
     CDS             complement(5989..7362)
                     /locus_tag="ACHA6Y_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8986544.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7388..7672)
                     /locus_tag="ACHA6Y_000426"
     CDS             complement(7388..7672)
                     /locus_tag="ACHA6Y_000426"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8986545.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7674..9689)
                     /locus_tag="ACHA6Y_000427"
     CDS             complement(7674..9689)
                     /locus_tag="ACHA6Y_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8986546.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9868..10173)
                     /locus_tag="ACHA6Y_000428"
     CDS             complement(9868..10173)
                     /locus_tag="ACHA6Y_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM8986547.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10262..11293)
                     /locus_tag="ACHA6Y_000429"
     CDS             complement(10262..11293)
                     /locus_tag="ACHA6Y_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8986548.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11500..12375
                     /locus_tag="ACHA6Y_000430"
     CDS             11500..12375
                     /locus_tag="ACHA6Y_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8986549.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12527..13369)
                     /locus_tag="ACHA6Y_000431"
     CDS             complement(12527..13369)
                     /locus_tag="ACHA6Y_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8986550.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13461..15338)
                     /locus_tag="ACHA6Y_000432"
     CDS             complement(13461..15338)
                     /locus_tag="ACHA6Y_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8986551.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15335..16756)
                     /locus_tag="ACHA6Y_000433"
     CDS             complement(15335..16756)
                     /locus_tag="ACHA6Y_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8986552.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16759..20148)
                     /locus_tag="ACHA6Y_000434"
     CDS             complement(16759..20148)
                     /locus_tag="ACHA6Y_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8986553.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20405..21796
                     /locus_tag="ACHA6Y_000435"
     CDS             20405..21796
                     /locus_tag="ACHA6Y_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8986554.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21869..22516)
                     /locus_tag="ACHA6Y_000436"
     CDS             complement(21869..22516)
                     /locus_tag="ACHA6Y_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8986555.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22524..23210)
                     /locus_tag="ACHA6Y_000437"
     CDS             complement(22524..23210)
                     /locus_tag="ACHA6Y_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8986556.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23216..24289)
                     /locus_tag="ACHA6Y_000438"
     CDS             complement(23216..24289)
                     /locus_tag="ACHA6Y_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8986557.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24511..25185
                     /locus_tag="ACHA6Y_000439"
     CDS             24511..25185
                     /locus_tag="ACHA6Y_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8986558.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25190..26530
                     /locus_tag="ACHA6Y_000440"
     CDS             25190..26530
                     /locus_tag="ACHA6Y_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8986559.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26679..27665)
                     /locus_tag="ACHA6Y_000441"
     CDS             complement(26679..27665)
                     /locus_tag="ACHA6Y_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM8986560.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8096 a         5247 c         5041 g         9391 t
ORIGIN      
        1 atatgaatca ttgtatttgc cgttgcggaa ggatttttta attaaccaca gataaacatt
       61 gataacgatt cgcttattgt aatcaacaga ttaaatttca tatgttcata tgtacggata
      121 cattttcgga ccgattaatt ttaaccattc tatttatttg cttacctatt agatatcaat
      181 aaaaaactgc ttaacagcta tttcagctgt taagcagttt taggattttt agttcatatt
      241 cattgtttct tataatacag tgttcaatac ttctcttaaa gcacttgttg atttgctgaa
      301 cattgctttt tcttcttcgt tgattgataa ttcaacaact tcacgaacac cattttcgtc
      361 aacgattgat ggcacacctg taaagatatc tttttcgtcg tattcaccag ttaaataagc
      421 agatacaggc aatacagctt gttcgttatt caagatagct tttacgatac gtgcagtact
      481 cataccaata ccatagtaag ttgctttttt acgatcaatg atctcataag cagcattacg
      541 gactttatca gcaatcacat ctaattcatc ttgggcaata cgatggtctt tttctacaat
      601 ttcaaacact ggtttaccac cgacagttgt atgtgaccat gcagcaactt ctgaatcacc
      661 atgttcacct aagatatagc cgtggacgct gcgtgggtca acttttaatt tcaatgcgat
      721 ttctttacgg aaacgagttg tatccaatgt tgttccagta ccgataacac gtgatgttgg
      781 cagaccagat tcgttccaag cgatatatgt caaaatatct actgggtttg aagcaacaac
      841 gataataccg tcgaatcctg atcccatgat ttcttttacg atttggcgca tgattgacgc
      901 atttgtttta actaaatcta aacgagattg accaggtttt tggttgatac cagctgtgat
      961 aacaacgata tcagcatctt tacattcttc atagccacct gaccatacag ccacattttc
     1021 atcgccccaa gccataccat ctaaaaggtc caatgcttcc ccttctgctt tttcttgatt
     1081 cacatcgatc aatactaatt cattagagat tccttggttg atcattgcat aagcgatact
     1141 tgttccaaca aaacctgtac cgacgattac tactttacga cttgtttttt tcatgaataa
     1201 acagctcctt ttcataacta tttgttactt tcatgaaaag tatagtcttg tttattcatt
     1261 ttatcaacat gttaattata atagttgtta ttataatttt tgatggaaac gaattcctga
     1321 atacgcataa cagataaatg acaaatgttt acacctatat cccctgataa tacaaccaaa
     1381 agtttagaac tgaaaataaa tccaaaaaaa ttcgctgcaa cgatataata agacagcaat
     1441 cgcacctaaa acaattttcc ttcatatgct ttagtgaaag attttcttct taatgtttat
     1501 ttctccattc attgggagta catgttgaat acttcttgaa taatttgaat aaagaagtcg
     1561 tttgtttata tccgatacga ttagcgattt cttccaacga aaaactcgtt tcttgaatca
     1621 gttccaacgc aaagtcgaat tttttcttta gaaccagttc ttgaaaggaa gcatcaaaat
     1681 acattttcaa tttattacta agatagaccg gattgtagcc aaaatgagct gctgtttctt
     1741 ttaacgtaca agaagtaaaa tgcttatcga tataatctaa tactctaagc atctcatttt
     1801 ctgcgcatcc atctgctgat gaaaagcttt gttgttctaa taaatgagtc aattcgatca
     1861 gtaatagtga tgtatagaga tttaacgaac ggatcttatg actgtatgta tcaaaataaa
     1921 tcaacagcat attttcaatc aaatgcgtca aacgacttgt ttttccgccg cgaaaatgaa
     1981 tgaattgtgt atgttcccct ttttcttttg atgcatttat caaaaaagaa gaaagtagat
     2041 tatttgtgct tgccaaatga ttcaatatct ctgtagataa ggtttcttct tttaataaga
     2101 tgttgattaa aatatcctct tgccctgtgt attcgattct ttgagtagct tctttatcaa
     2161 tgatcaccac gtcactttct tccatctcta tcctttctcc attaataatt tgtaaacatg
     2221 taccgctata catataattg atttcaacaa aatcatgtgt atgctcggga acttccgcat
     2281 atcgacggtg tttactgata tgaatatttc cctgttgaaa tagatcccct cggtggattt
     2341 ttggcggtgt ctgaagatgg taagctaagt ttaaatcatt gatattcatt ccttgttcac
     2401 gctgcagagt ttcgattgat tcttcgttac gcaaaaaaaa tgtctaattc agctagttcc
     2461 aattgactcc ctcctttttg taagcggttt ctttcgctta ttagttcacg ctgtactgct
     2521 attatatgca aagctatttt tggtttcata aagactttct atggttggga catgaacttc
     2581 ctgtgattcc gctattgaga gaataaccac ttcatactat atttttgata agcgcttaca
     2641 aaaatgaaag gaacatgctt atgaataaaa agatgatgaa attttctctg ctgtcagtct
     2701 ctcttatttt gacttctgct ggtgcagtca cgtctgcaat tcccagtatg tcagctactt
     2761 atgccacgcg ttctttatct gctgttgaat tattaacgac tgctccctct tttatggtca
     2821 ctatttttgt tttgattagt agttttattg ctaggaaaat cggacaaaaa caaactgtga
     2881 tcatcggatt agtgattgca tcgatttgtg gtatcctccc cctcttctca caaagttaca
     2941 gtattgtctt agcttcaaga gctggactag gtattggctt tggattgatc aattctttgg
     3001 cagtcagcat gattgctgcc ttttatcacg gagatgaacg agcctctatg attggttttc
     3061 aaagtgcatt tcaaggattt ggtgcagctt taatgacatt tgttgcagga cagctagtac
     3121 agttcggttg gcaatatacc tttttagtct acgccatcag cttacctatt cttgtcttgt
     3181 ttgttttgtt tgttcctaca cctgaaacaa ctgaacttca atcgcatggc acagccccaa
     3241 caagacaagt aatcaaaacg aatcctaaaa tgattagtta tggtattttt ctcttgctgg
     3301 tcatcgtggt atataatgct gtatcgatta aattagccac agtcctgacg acttattctc
     3361 taggaaacga aactaatgca gcgaatatct taacaatcat gcaattggca agtatggctg
     3421 ccggagtttg ttttggctgg cttcagacaa aagcacatcg ctatatgctt acactttctt
     3481 tactactgat gggaatcggc tttgtattga ttgccactgt gataaacctg tatgtgattg
     3541 ggtttggtgc gatcctaaca ggaatttctt tttcgttatt cattccttac ttattcaacc
     3601 aagtaagtat ccaagcgccc aagtacgcgg agagtttcaa tacttcaatc ttattggttg
     3661 gcgctaattt aggtactttc attgcaccat atggtttgct ctttctctct tttgcttctc
     3721 ttgggagtaa aacacttgct gttttcgtca atggcagtat cttgctgttg attagcgcgc
     3781 ttattagcct tctagtagta cacaggacga atagaaagac agtgataaga gtagatgaag
     3841 aaataaaaga acaattgaca acaaaataaa tttgatggag gtcgtgtaaa atgacaaatg
     3901 aaaaacataa agatagatgg ctttggtatc ctggagactt tgagatccgc catggactgc
     3961 tacaaaattt ccaacgggaa gaacgaggtt ttgactggcc agcgtactgg tacatggatg
     4021 attgccatcg gaacgtcaag tttaagagat attactttct tgatcaacct tccatgttta
     4081 aagtgacgat ccaaggagtt gggtatgtag aaattaatgg acaaaaacat ccatgtggaa
     4141 aatggctgac ttgtccagct ggtaaagcaa aaattcgtat ctttgtagga catacatcag
     4201 ggctaccagc gatgtttatc gagggagacg aagtaaaatc agatattggc tggacagcaa
     4261 gtaattttat tgaagaatat ccagctggct ggagtccgct ttatgtcgat atcgcaaaag
     4321 atccaaatca aatctattat caaaaagaat gcgtccatcc agtcactgaa acggagattg
     4381 gcggaggaat cttattcgat tttgggcgag cagtgaatgg ctgtgtagaa ctaatatctc
     4441 tggatgacca tatcgaatct atcactctat gttatggaga gtccgatgta gaagctatgg
     4501 atatcgatta ttgctactat aagcaaatag gaatgacaag aaaaatgaaa gctcgtaaac
     4561 gcgcttttcg ctatttgttt ataccagagg tttctactgg aatggttcaa cttcaagttt
     4621 ttcatgaatt tttatctctt tcgaaacaag ctgctttttc aagcgattct tctttactga
     4681 atcagatctg gtcagtatca gaagaagcct tcaagttatg tagtgatttg ttttttatcg
     4741 acggtatcaa acgagatcgc tggatttggt ctggagatgc gtatcaatca ttcatgatca
     4801 atcaatatct tttttttgac gaggaaatca ataaacgaac gatattagca ttaagaggac
     4861 aaaatgaaat caagcagcat atgaatacta tcgttgacta ttcgatcttg tggttgattg
     4921 gtatcgaaaa ccactatatg atgacgaaag actatgaatt tttacgcatc gtctacccta
     4981 agatgcaatc attaatgaga tacttgatgg agcaaacgaa tgaattaggg tttatttatg
     5041 ggagagcaaa agattggata tttattgatt ggtctgaaat ggataaagaa ggaacgcttg
     5101 ctgcagaaca agtcttatta ctgaaagcct atcattccat gatcacttgc ggagatatct
     5161 tagctgaaac atatggagag aacgaaaatt atcatactca tctctatcaa gaaaaatatc
     5221 aactattgaa acaaaaccta tttgactact tttgggatga agaacttggc gcttttatcg
     5281 attgctatga atctgggaga agaaatgtga cgcgtcatgc gaatattttc gctgttttat
     5341 tcgattttgc taacaaagac caacagcaat cgatcttaaa gaatgtctta ttgaatgaca
     5401 ctatcacaca gattactact ccatatttca aattctatga acaagatgct ttatgcaaac
     5461 tcggacagac gaatatcgtc taccaaacga ttcttgatta ttggggaggg atgttatctc
     5521 atgatgcggt aactttctgg gaagaatacg accctactca aacaggaaat gaaaagtaca
     5581 gtatgtatgg agatccttat ggaaaaagct tatgccatgc atggggagca agcccaatct
     5641 atcttttagg acgctatttt atcggtttgt atccaacaaa gccaggttat gaagaattta
     5701 tgatacaacc taatctagat tcatttgaaa cactgaattg tacgctacct atcaaagatg
     5761 gaaagatttc gcttgaaaaa acaaaagata agatcgtgat tacttctgat agaacaggcg
     5821 gaacagtgaa aatgaatggt aaaagttatc ctcttctagc aaaacagcca cttgtcttaa
     5881 caagctattc aaaaataaat gcataaaaca aaaaactgct tgcgacatat cagaagactt
     5941 taaatcttct tctgatattg tccaagcaat ttttattttg aagtttgctt aattctgttc
     6001 tttagaaaca atgtttgttc tttttccggc aaaaagaaag ctagataata cgacaagtaa
     6061 aaagaaagcg atgattccaa ctgtcaaggc tatttgaccg tattctccta gtttcccaag
     6121 tacaagtcct ggtactagat aatcagtatc ggcaaatgta gaagaagcga ttttaagatc
     6181 tcctaatact ggaaggataa ataatggcag ccaactaatc aataagctat tgaaagcaga
     6241 acctaatacc gcccctcttc taccgccagt cgagttgccg aagacaccag aagcagctcc
     6301 gcagaagaaa tgtcctacta ccccaggaat gataactgta gtttgcaagc ctatcatagc
     6361 aaacatggat agtgttccta caaagaagct aacaaaaaag ccaattaata cagcattagg
     6421 tgcatatgga aaaacgatag gaacatctaa tgctggttta gaatcgggta cgagtttttt
     6481 agcaatccct tggaatgctg gaacgatttc tgccaacacc attcgaaccc cttgaaggac
     6541 gataacaaac ccagctgtga atgttccagc ttggatcagt gcaaagatta cagcgttgtc
     6601 tccattgctt agttctcttt caacaaatcc aggaccagct aacaaagcaa gtgcgacata
     6661 tacgatcgac atcaagagca tgatcgatac agtagaatct cgcaaaaagc tgaaactttt
     6721 aggaatcttt atgtcctctg tagaacggtc tttgtttcca aaaaactttc cgatccatcc
     6781 actagaagca taaccgatat ttccggtatg ccctaacgca acttgattgt ttccagtgat
     6841 gttcttcata aacggttgac aaagagcagg cgtaatagtt aatagtgttc cttcgaagac
     6901 cccgcctaat aagatcatcc acaaatttgc agtaaacccc gcgcttacta aaatgactgc
     6961 tgttagacaa gaaacataaa gcattgcttg acctgtaaga aaaatgaatt tatatcttgt
     7021 gaatctagca agtaaaatat tcacgatcat ccctactagc atgatcaaag cagtaggtgt
     7081 accgtattgt tgcaaagcaa tcgccactac tgcttcattg ctaggaacta ctccttgagt
     7141 atgcagagct gtttggaaca tcgaagcaaa aggggttagt gaattactga ttaaaccagc
     7201 accgccgatc agtactagga atccgacaaa tgttttgata gttccttgga tcacttctga
     7261 aaatttcttc ttctgaagaa cgagaccaag cagtgcaatc aaagaaacga ggatggaggg
     7321 ctgactgacg attgacacaa acaaattcaa caatttatcc attgatagtt cctcccttga
     7381 atcattttta ttcttttgac aagatcttcg ttactttttc ttttacttct tcgctgtcaa
     7441 tgatcgattg caaaggtacg agcttttcat caggtataga agttagcgta tctttcaaag
     7501 aatgctcaat gaagaaataa tcagccatat cgctagatac acttccaaca tccgtatgac
     7561 ttacttcaaa ctcgtccaca tctgctccaa gatctgcaag tgcttgtttg atgttcatct
     7621 ctaccataaa acttgttcct aatccacttt gacatactgc aaaaattttc atatcattcg
     7681 tctccttttc ttatgatcgc taatatttct tcttttgttg atgcgtgtaa taaatcttga
     7741 agattttgtt tattagaaag tagctttgtt aaatttgcca aagcccgaag atgtgcctca
     7801 ttatcgatag cagctaaaca gatgaacaaa ctaatgggat gggctggatt atcaagcaga
     7861 tccacaggtt gttgaagttt cagcaaagac atccctactt cgtttacgcc atcttctgga
     7921 cgagcatgag gtaatgcgat gtgatcgcca atatggataa acgcaccata ctgctccacg
     7981 cgctcgatca tagctttgat ataactttct tctattttat tttctgataa tagcggctgt
     8041 gcggcaaatg tgattgcttc ttgccagttt tttggttgat ccgataattg gatcatttct
     8101 tctgttaata attcagatag cataggacgt tgatcctcct tcttttctaa aagtttgttc
     8161 attttacgat ttaatacttg atagagcttt tcttcgtcta cgccttcttt taattcgata
     8221 taaggcagta gcgccgacaa caattcttgg gcagaaggca ttgaaaatcc tggtatcagc
     8281 caatctcttt gtacttgatt gatcagctga tttttttcca agtcagtcat gattggatgc
     8341 agaacataga gagacttttt cttatcagta agtgtgagag gtacagtaga aaaaataata
     8401 tcgtaagaag tttcgtcaac ctcgtgtaag cggtcagctg aattagtctc tgtaaaaaag
     8461 atcatcggga ataattgctg aagttcactt tttaagatca atgaagagct aatgccactt
     8521 ggacagacga tcacggctct tatttttcta tttctttctt ctgcgtccgc ttttctgatc
     8581 tctccgccaa atagtatagt gaaaaaacca atctcttctt cagggatgct cttccctatt
     8641 cgtctttcta acggaagtaa tgcttttctt gtcatttcgt agatggaagc atattgatgc
     8701 ttaatttgat cgatcatcac attaggtaaa taaaaagaat aggaaatacg aaaataggca
     8761 gggaccaagt gataaaagac attcatcaat aattcacgaa acgaagcaaa ttctacggcc
     8821 atcaggaatt ccatgcgatg gatgacttct gaagaacatt ccagcaaaaa atcaagtgca
     8881 ggctcctgga catctccttc aacgatcgat gctaagcaaa gagagaaaaa tataatctcc
     8941 gaattttgat gttcggaata gccaaggcca gtcaatactt gtcgactaat gtcagtgact
     9001 gcaagagaag ccaacagttc agcatctttt accagttcca tggatgtttt tccaattcga
     9061 atggttgtca aactcaaata ataagctaat tcatccattc gatttggtgc gaatcgaaga
     9121 tctgatagtg aaatcgtttt atctagcaaa tctctaactt tggcgtaata atcaatggaa
     9181 agggttgcgg ttaaatacgt gagcccaaat cttccagcaa gtgactgcat caaacgagca
     9241 gtgcgatttt ttgcttctct tcttaatatt gcttctgacc ctagcagcct aaaaccagtt
     9301 ttcctagtat acgacagctg acaatcttcc acttctaatt catttcttat ctttttgata
     9361 tctgacagga ctgtcccttt agaaacctga agaaaatctt gaaaatgaaa aacagaaaca
     9421 gacgttgcag tcgaataaac catcaaataa agaagatctc ttcgctcttg ttcggtatac
     9481 acagcttcaa atcgagtaat atccaaaaat ttttctgccc agtcgattgc tcggaatttc
     9541 tttatacgga tacgggtatc atccacatat tcggcaaaag gagttaattg tttgatcgcc
     9601 tcgcttgtat ttacttcgat gatctctgct gctttttcta gactgatttc tcttggtgtt
     9661 ttcaggagat aactaatttt gtagttcaca gcgctgttca cctcctactt atattctagt
     9721 atggtagcgc ttttaaatcg aggggtgttt ggtacaactt acagttggat tttttttacg
     9781 actgtaccga acgatttcta tcgaatggat acagaaaaaa agcaggtgac caaatgtctc
     9841 ctgcttttat gaattgattt cgtccgatca atgatgatca tgtttatttt ttgaggtcag
     9901 ttcttcaaat ttttctttga catcttcaac cgctttttcg atatcttttt caaagtgatt
     9961 gggctcatta ggctttgttt cctcttctac agaatttgaa ccagataaag ctgtttcttt
    10021 attttctgtt tcaccagagt ttgtgccagc taatgattct tctttgtctt ctgtttcacc
    10081 agaattagtt cctgctaaag ccacttcttc ttctgtttta ggggccattg aacctcttaa
    10141 tcgtttgtgt ttttcaccgt cataatttgt catgttggtt cctcctagta gttctatact
    10201 ttttcatcgt acaataagac agtttatgcg tcaaaaaaca aacccgattc ttacaaatat
    10261 gtcatatttt tccttctgat tcttgaatgc ttttgattcc ttcttccaaa attttcattt
    10321 gtaagattgt ttcttcatct ttgatgactt tttctgcgcc attaactaat gtctcgtaaa
    10381 tcccttcgta gacacggctg taatcaccaa cctcggaaat tactttttct tcatgataac
    10441 tcccttgttc atctatatag gtcaatacac cataatgttc gggcaaatcg ataccaaagt
    10501 cttcatgatc aggcatgtaa aacattttca gatgttcttc ttgtcgatct tttgtttgtt
    10561 tcacgaacat cccttttttt ccgtaaacga cgaaacttgg acgttctttg atgcggaaat
    10621 aactggattt tatcgaaatt ttcatatttc catagaaaaa atccagatcg aagtaatcat
    10681 tcattcgtcc tttccctaaa agttgacgta catcatagct gatatcatct ggttcgccga
    10741 aataagaaag aacttgatct actgtatgac aagcatgtcc atataaatag ctgttcgcag
    10801 tagaaaattc atgcgtattt tctggtactt ccggtctgaa ataatcataa tgcatttcta
    10861 cttctaaaat atcccctaat tttccgcttt cgattacttt ttggactgtc aagaagtcag
    10921 aatcgaaccg tctgttttgg taacattgga taaatagacc tttttctttt gctaatgcaa
    10981 ataactcttc tgcttccttt gacgtttctg caaaaggttt ctctactagt acattcttac
    11041 catgttcgag tgccattttt ccatattcat aatgaaattg actaggtgta gtgatgacaa
    11101 ctaattggat ctcaggatca tcccaaagat cagacagctg atcagtatat tcgatgtctt
    11161 cgaatgtttt ccacactggt tcagatgggg aaaaaatttt tttgatattg attttgttcg
    11221 taagttttct tgaaaaaggc aaatgatagc gatttgtact ctttccattg ccaagataag
    11281 cgattgtaag catgagtggt gcctcctttt attgatgttt tcagtatatc ctagaaaagc
    11341 gcagaaagta tttataatag ctattctggt aaaaaagttc agaaaaacac ttgttatcat
    11401 tcggtttggt gaaattaatg ttcaaagtaa ggataaaaat caaaaagata gtagcggaac
    11461 caagtgttta tcagtaagat agaatttgga ggtcagatca tgcttattga agaaaaacta
    11521 acaaaacaag agttgtttac cacgacagag aaacggattg tcgattacat ccgtaggaat
    11581 atcgaagctg cagtctacat gacgattgaa gaattagcaa aagctacgta tacttcccat
    11641 tcagctatca ttcgtttatg caaaaaaatg ggattcagcg gatttaaaga attccgtttc
    11701 gagctggggc gggaagttca tcaattgatt gctcagttca atcaaacgat tgatcctaat
    11761 tttccttttt caagtgaaga tagcactcag caaattgcaa agaaaatggc ggatttatcg
    11821 atccagtcaa taaaaaaagc acaattgcag attgaacatg aagatttaga tcaaatcggt
    11881 aaagtattga acaaggcaca gcgaatcttt ctttttgcaa aaggtgattc tcaaatcacg
    11941 gcaaggaaat tccagaataa aatggtcaag ttgaataagt ttttgatcat ggctgaagaa
    12001 tacagtgatt cttcttggaa tgcagccaat ttgactagcg aggattgcgc catttttatt
    12061 agctacagcg gtcgcatcca tcattatgaa cgaataatga cctatttgaa gcatgtagag
    12121 gcccctacct tgctgattac cggaaatcag cattcagaaa tggccaaaca ggctgatatg
    12181 tgtcttgtta tatcgcagga ggaatatgat tttgctaaag tagcgacttt ctcctctcaa
    12241 attgccttcg attacgtatt aaatacgtta ttctcagtga tatattctca aaattttgaa
    12301 gagaatatcc gcaacttgaa aaacaaacaa gaattgatgc aaaaaggcct tcttaaagaa
    12361 tatcggaaaa cttaagaaaa gatcctcatt tagtaataag aaagatagtg tttgataggc
    12421 tctctttgat atcatcataa aaacaggctg aagttttctt tttcaaacct cagcctgttt
    12481 gtttacggaa cgattcgaat agatatatta tagaaatgag gaatagctaa cttctgtttc
    12541 ttattttgaa cgattgacga aaaagatata taagaaggag gaaaatcagt atacatgcag
    12601 ctaacaatcc atacaggaaa aatcgtttgt tctcggatac tttctgtact tctttttgaa
    12661 cttcttttgc atgataagga acccggtgcc ctctgacaag taagcgatga ctgttgacca
    12721 tataaggtgt acaagttagg agtgtcacga aatcctgccc tttttcgatt ctcagatctt
    12781 ttgtatcagt cggttctacc gtcttgatct gatcgacttg ataagcgagt gtccgtccat
    12841 tgatttcgat atagaaagaa tcacctaaag tcagtttcgg taagtctgtg aacaactttg
    12901 cttgaggcag tcctcgatgg ctggaaataa cggcatgtgt attttctccc cccgtaggat
    12961 aagacgttcc ttccaataat gaggcacctt tttctagcag cacagtattc gtttcatcaa
    13021 aaattggcaa atggacattg atctcaggga tcgtcaaaat accgatcgta tgtttctcaa
    13081 aataggttct atcctttttc gcaggttctt ttttcttagt aaagggatct gcacctggat
    13141 ttccaccttc ttttgctaat tgctggtttt tctttgtcat gttttcttgg attttagcca
    13201 tgacttcttc attttctttg accgcttgtt gctgataatg agagatgatc tgctgatcca
    13261 gataattatt cagcgcgtcg ctaacaaatg gataagctag tatccctatt cctgagagaa
    13321 gcaagaaaag catcagcaga tctagaagtt tccttttctt tggtttcatt tgtgttctcc
    13381 ttgtgatgac tgtttgataa taaaagaaaa aggagcattt gcgtaatccg ctgctccttt
    13441 ttccaattct tgcaaaaaga ctaaatctga ctgtgcttgc gtctagcaaa gtacagtcca
    13501 gcaatcagta gaaggactac tcctgcaccg atatacacat agattccctt accgcctgtt
    13561 gaaggaagtg tacctttgtg tttatttggt attttttcag gagaaatcaa ctgtcctgct
    13621 gttacataag attgttcatc gatagtaaat gctacacggt ttgttaatgg aacatatttt
    13681 tctggcgctt tcgtttcttc cagatagtac gtgccatatt ttagacctgt cacatcgatt
    13741 aaaccgttac ttgtggttgt aaaaaccgtt gctgattctt tcgccgatac ccagctgacg
    13801 gcttttgtgg atgggtcgat cgataaatat ttcgctgtgt cactatcttg atcacgaacg
    13861 acgaattctg ctccagcaag tgtttggtct gatgtaacgt caccatctac tttaacaaat
    13921 cgtttgcccc cagtaacgac atcgactgac ggtggtgttt gatcatttgt atgcccgtta
    13981 tcgacatttg cttggttaga aaatcctttg gttggatctg ctttttcgtt caaatgcata
    14041 tagtaaacga atttcaatgt accgccagga gttaatgaag gaatataatt cggatcaact
    14101 gaaaccgtga atccgtctgt ttgctcagtg acagaataat ttactgggtc gatttcttta
    14161 tttccatcat ataaggcata agcatacgtt cctgaagaat cattatcaaa tgttaaagca
    14221 gcgtcatgag tatcgataag tttgaatgtt gtgtacttgt tttgcgttcc ttctttatca
    14281 gcgataccta atgggatatt gacagaaatc tcatattgaa ttttttcacc gattgcgaca
    14341 tcttttccat tcaattgagg tgttgtttta tcaactttag acgtatcatt tttgatggtc
    14401 ttttctactg gtgtttggct atttgccagg atctcaaaag gcgtgattgt ttgcttttca
    14461 atcatttccg cattatcagg agcttttact tcttctaaat tatattttcc aacttctaaa
    14521 tgattaacga tcaactggcc tttttcaata gatgcctcgg caaagtcatt gttgccgatg
    14581 tcataagaat gaccagtaat gaaatgtttg gctttggttt gatcagttgt ccaagtgtac
    14641 aatccatctg tgacactttg gatgtatttt ttgacgcttg gtgttccttc ttctttagaa
    14701 ataataaatt ctgctccatt tagtgcttcg ttttcggcag taccgatttt tgtaactttc
    14761 aacgttccat cattaccgac tgtattttta gggtagagat ggatagtatc tagttcttct
    14821 gtcccgtatt tataagagcc atctgcttgt ttgatcatct catatacagg gaaagctaaa
    14881 accatgtttg cggcagctga cactccgtct tttggttctt ctttgatcgt gtagactgca
    14941 tctttcccat tttgtttttt aggtaaagat aaagtgacat ttccatcagc atctgtcgtt
    15001 cctgaagcaa ctggtgtacc aggagtcaaa gactggactg cttgttttgc tgcatccacg
    15061 gacgctcctt tatctcgttg cgcataaaat tcttgagtga cgttataaac tgaaaatgaa
    15121 atatcggcta atccttggta ttgatcgaat tcgctcattt ctttcccgct gttttggatt
    15181 aaaggatcgg gtaaatcagt cattttcttt ttatgaagga tgacttgagc tgctgtctcc
    15241 tctgcaaaca ctttttttgc gccgaagctg tttgtgagta atggtaaaat aaggaaaagg
    15301 actcctaaca taacgtttat ttttttatga tttttcattt tttcactcct gttctttttt
    15361 tgataaacaa ccagattgca aagcttgcga ataccatacc aacaacgcct aaaagtgtat
    15421 ttttcgtttc gtttgttttt ggaagaaatg gacggctatt ttctttttta ttgactactt
    15481 tcaatatact gtccgcctct ttatcctcag aaagaaaagt tgaaatggta aacggaattt
    15541 ctgtttcact taaaatataa ccagaaggtg cttcgatctc ttttaatcga taacttcctg
    15601 ttttcaaccc ggaaattgaa aaagcgccat ttttatcgga aatcaggtga agcgcatctg
    15661 attcgttctt tgtccacttg tatccgttgg ctgtttgctg gaggtaattt cctgctttgt
    15721 ttttaataag gaatactgcg ccagctaatc gtttatctgg tttatttgtc tcgattttga
    15781 caaattgctt actccctgta tatacagaga ctttctttgt actgacacca aaaccaaaat
    15841 ccagtgttcc gttgttgttg atttccttgt tcgcctgtgc tgtactgctt agctgcatct
    15901 gataagaaat ggtcagtttt tttccagcat atttttccaa cgaagggatc gaaaataaaa
    15961 catcaaatcc gtgaggtttc ttgtgtatga caaagccttc tgtcagcttt tctccattcg
    16021 atgaaatcgt taaactatta ggtaaaaacg ttaatgcttc atccgcacta tctgacaatt
    16081 cgaacttttg atagtcaagg atatttacag gaattgtcgt atgcaaggaa taagtgattt
    16141 tttcaccaat cgtaaaatca tttctaggct cttctaatac cttctcaaaa ggtggatcag
    16201 ggtagtcgtt ttcttcattt ttaggataca aatggatagt tgaaagtttt tgattgtttt
    16261 gtccatgtac aggaagaaca actaccatat tctctgcctt ttctttgacg acttctggta
    16321 ctttggattc aatgaaaaga tacgctttat ctcttttttc ttgatcttta gcggccagag
    16381 aaaaagaagc gataccatct tcgttgttaa gagttgtagt tgttgcttct gcggtaaaca
    16441 taccggaaga cgcaccgttt tttgcgatct ctgcttgtgc ttcttctacc gttttgccct
    16501 tttcccgtag atggtaaaaa gaatctgtga catcataaac ttggaatgtg acaccattta
    16561 atcctcgata cgtttgtaat aaagcttttt cttgtccgtc atttggatga tttttcggca
    16621 gttgcccgtt tgggaaaagc aatttgtgta aagtaatctg aaccatttct gtttctgttt
    16681 ctgttgctac ctgagtaaaa gctggtttaa atagtaacaa gaagagacac atactaagcc
    16741 aaccaagttt tttcatattt agctgccccc tctttctttt ttcaaaaaaa gataccaaat
    16801 agaaaacgca caaccaatca tccctactag atagattcct aaacggccaa ttcctcccgt
    16861 ttcaggtaat ggtacttttg cctgattcgt gatgtctaaa gaaatctggt tgtttttggc
    16921 tcctggtgac agaacagatt catgatcttg tccatccact tgaattgacc catcttcgtg
    16981 tataactata gtaactggct cttttagacc ttggtatcct tctggtgtaa aagtttcggt
    17041 cagcgtataa gttccaggtt ttaaattttc gaatagaaag gtatctgttt cttgtccatc
    17101 ttttggcgat tctaagtcca tttctggtcc ctgcagtctg aattttgctc cttttaatgt
    17161 ctgtccgttg tcttcttttt tgtgtaccgt tagatcaaac gcttttagtc gattttgatg
    17221 agtgagcgtc catacattat ttgtcatctc ggtatttttt cccgtgtaaa tgatttttcc
    17281 atatttatcg atttggaatt cataatttcc cggcgaagta tcatatccgg caggaccagc
    17341 tgtttcaacg actcgatatt taccaggtgt gagtgaatca aaactggcaa gacctttttc
    17401 attcgttgtt tggctgtcaa ttggctgata agtaccattt gcttcttttt tctgtaggga
    17461 aaatgctgct cctttaagat ttatctcttt gtccgtccct tgcatcgcat acttacgtac
    17521 tgctacagga acttcaacaa aaggattttc aatagtcaac tggatcgtat catcggaagt
    17581 agtgactgtt tttccatcaa tggttaccgt accatcagaa gcgatcttga tttcccaagt
    17641 agtctttttg cttaatccat gcccttctgg agcttttgtt tctgtcagcg tataggtcat
    17701 ttctttttgc aatttgacat tttctggaag agaataggtt ccgtcgccat gatctgttag
    17761 taaagtatca atagaatctc ctgttaattt gaaaacagcg ccaataagat ccttttcacc
    17821 tgtagaggaa tttttcgtta ttttcaagtt taacggtgtg aattgtttag tatttttcca
    17881 tgtcattgca tctatttgtt gagaatcgta tcctggtaca ggtaattctt tgattgtttg
    17941 gtaactgaat gcttgacctt gattattgta ttgaggtagt gataatatct cttgataact
    18001 ttctccgcta tttgcagata atttgtcaat gtctgcacgt tcccacgtat ttgttgtatc
    18061 tttagctggt ttaatgattc gaatatatcc gtttttccaa gctgcagcat ttgtcgtatg
    18121 ttcccgttga atctcaaaag taacttgatc tggacgatca gctagattgt tgtcaaattc
    18181 ttcccataac tttttgatgt gaagactgac tccgggagct ttagcagatg gtataccgaa
    18241 ttcagctaat tcattggtat caatgtttgg ctggaaagtt gtcctgccgt tcatttgata
    18301 ccagaaattt ggatggaagt cctcattttc tgtttggatt ctcacttggt aatggatttg
    18361 gatttcttgg tcttttccta aatagatctg attgctcttg atggtatttc cttctatgga
    18421 aatagatgga gtgaccgttg taggacttgt ccccacactc ttgactgaaa gtgtaccagg
    18481 ctgataaatg aatggttctg cgattggatc atttatttgt ccattgctta cagttgctga
    18541 gatctgtaca gcttttttgg ctaggtattc agagatatct gttgcatgat cagctgattc
    18601 atagtaaaga tcgccttttt catctgatga aaccatttgt cgcatacgag actctacttc
    18661 tgcttttgag agaccagctg ccggatcgct ttgaagttgg atgccaagac catgtatttc
    18721 gattcctcgt tctttgagtg ccatcgcttc tccgatggtt gcgataaacg tactgtcgat
    18781 tcttctggat agattgtttt ggtcaggtgc atcatagatt cttgagatta gagaagtatt
    18841 tcccggccga tcttgcgtat tagaaaactg agttccgtaa taattgctgc ttgattgtgc
    18901 gtgtacccgc tgtactttat aggaaaatgt tggtacacca tccgtcagca aaacgatcac
    18961 ttttttatgt ccatttggaa cggatagcat gcttcctgca tctcttagtg ctttttgtgt
    19021 aaaagtacca ccatttgtcc gtgaaggtgt aatggatttt acttgatttt tcactgaatc
    19081 aaatgaaccc atctgtactg caccgttact gtagctatat ccttcgcttg agtagccgac
    19141 atatcccata ttgattttgt ctgtgatacc gctatctgct aaagtatcga caaaacgatc
    19201 gacaccaatc tttacttcac cgatccgatt attgtcgttc atacttcctg accagtctac
    19261 gaccaatacg agatcaagag gcgtgatatc tttttgtaca tttccacgag cattcaaata
    19321 aacatcaaac aatccgggtg tacttgtttc ctttgcatac tttcgtatcg catagtctgc
    19381 ttgattaccg gttcctccat attcgatata cgaatgggtc cgatcattag gatctccatt
    19441 ccaagaattt ataccatccc attgatttgt tccgttttta ttgccttgat gatcaagaac
    19501 atttgtattt cctgtaggtt gccaactggc tttaggataa gtgccctgat catctgttgt
    19561 atattctgta ggaatcgttt gattagatgg cacactcaca gctggcaagg ataatgtagg
    19621 caatgtaaat gggctgacag aattttcgtt ttcttttgat gattcttctt ctatttcgtc
    19681 tggctttggt actgacacat ctgtatcttt cttattttct gtctcatcaa taatgtcata
    19741 ttctttatta tcgacgatca gcttgtttgc ctggctgtgg taaagtgctg gatcgattgg
    19801 aaaactcaga tcgatagttt gtttagtgtt tgatgagatt tccaacgtaa ttttcccgct
    19861 atttacctga taagcatagc catctccttc ttttctttcc acatcactta agtaattttc
    19921 gactagacca atttcttttt ctatcttttc agcactggtg ttattcgcat ttagcttgat
    19981 gttcagtgct ttttttccag actgatccga aatgacttca gctgaaattg tttctaaaac
    20041 tgttggatca tctgtagtgt ctgctattgc agataatggc acaaagtttg ataaactcaa
    20101 tattatcagc atgaaaataa ctttcagttt cttccctgtg gtcgtcattt ttcccctcct
    20161 ttttcttttt ttctgttttg acgtgaatag tgaaaattat taaaataaac aggcgttaca
    20221 taattttatt tattttaata tgataattat tatacgaagg gcgctaaagg gaatcacacc
    20281 aattttcata ccaattggtt tcatttatga agttgattcc ttcgattaat gagcgtttca
    20341 ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg ttgttggaat taaactatag
    20401 aaagatggaa aaaatcattc gaattctgtc catttctgac aagtggatga ctaaaaaaga
    20461 acttgccaaa cgaattggta gttcagaatc tacatttgta aggtatatcg aagagatcaa
    20521 aatccgctgg ggaacggatg taacgattca atcttcacaa aaattggggt atcgtcttga
    20581 acatttgaat gtaccagctt atttaaatgt tctgatggaa atgtcgcaaa gttctacaaa
    20641 cagtcgttta ttaaatgaga ttattgtcaa tccgggtaaa acgattgagt attattgtga
    20701 aaaaatttcc atcagccggt cttcttttac acgtaagttg aaacaatgca atcaaatttt
    20761 agaaagctat ttattaaaag tgatcgttga ccaaggtttt cagttaacta gccttaaaag
    20821 tgagctccag ttgcgtatct ttgttacctt tttcttttta agttactacg gaagacgcac
    20881 gttgccattt ccaatcgaaa aaaagaagat caaagaaatt atggtcaaga ataattgtca
    20941 gatgaatttg tttcaggaag aaagcccata tgagcaatct ttctttatta tgtattttgt
    21001 tgtgcatctc atgagagaat cccaaggaac tccatttgaa tggaaagctg gagactctta
    21061 tgtacagttg ataaatatcg ataaaaaaga cttcttaatc ttgaaaaatc tcttaggaaa
    21121 attgaagtac agcagctata taaaagtagt gaactatttt gtcaatacga ttttttcaga
    21181 attttcggag aaagaaaaaa atgaaatcag tgagcagata cgaaaaaata tgtcctactg
    21241 ttcgttttca tccgctgatt gcaaagaaaa aaacgaagaa tatgttgttc aattattaac
    21301 gcaaatttat tttttctccc gtattttccc atacgatacg actcatttga cgatccgtgc
    21361 aacttacttt gcacagcaga tgcaacaccg ccagatacgt ctatatgctg gatttcgtgc
    21421 agaacttcaa ttttattcca ctctactgaa tcagaatctg atttattatt tatctagcta
    21481 tatcttttgg atgcttatcg gtaatcctga aattcaccat tatacttctg ataaaaaaat
    21541 actgattatc agcgacttaa gtcttcgaca tagtcaatat attgaagaat atatcagtga
    21601 tattcttgct gtgcataaaa ttcactctga gacaactgct attactgaag atcagctgtc
    21661 gaaatacaat cttctcgagt atgatctgat cgttacgaat caacccattt tgaatgccca
    21721 tgttcctcat atattgattg atgatagtgt gtcattcgca aatgaagaag aactcatccg
    21781 cttattcgag ttatgaataa tcaaaaaaca gctatgtata aaccaccctg ataaaaattt
    21841 caggacaaat acacatagct gtttcttttt attgtattcc ttcagcaatc caacgagatt
    21901 tatagcgaaa gtcttttaac aactcttctt ttgtccaatt atttgcaatg agatccgtca
    21961 aagtgttcat tacgagggtc atggaaaagt ataggaaact aactgcttct tccgacgatt
    22021 gaatggagaa atgattgatt cttaataatt ccagccattt ttcgttcatt ggagaatcag
    22081 ccgatagttc tctacgtttg gttgtctgct gatcatttcg ctgggttaac aagtataacc
    22141 tttgccaata gatactttcg tgatctaagt cgcagcacca ctctaaatat ttttcgatcc
    22201 ccaaaaagaa atgttgtttg ttttcgctaa tgaattttaa gatatcttga tggactatgt
    22261 gagaactgct tttgatcgta tacaaataag catcttccaa atcttgaaaa tatttataaa
    22321 aagcaccgcg cgacatcccc atcttgtcaa cgatttctga gactttgact tggctgatgt
    22381 gctgtgagca aaatatctcc agtaataggt catcgattct ctgtttcttt gcgttcgata
    22441 aattgaaaaa agtttctttt ggcatccaca tctctccttt atctctgatt atacgtggat
    22501 agcccttcat tgtcatgaag gattcattcc ttctttttct cttcttctgt caagtaccca
    22561 tcttccattc gataaacgcg atcactccat tgaatcatac ggacatcatg agtaaccata
    22621 atagtcgctt tttgtttctc atgagcttct ttaactagta atttgacgac ttcatatgca
    22681 tgatctgtat ccaaactagc tgttggttcg tctgctaaga tcaagcttgg ctcattatat
    22741 aaagcgcgag caattgccac tctctgtctt tctccaccag aaaggtcttt aggataacta
    22801 tttttaagtg actggatatc taaagattgc agcaaggaat cgatttttgt tgaatcttct
    22861 ttttttgaag taacttggtc aactagttca aattgttcct ttaccgttaa aaaaggaatc
    22921 aaattagagc tttggagaat aaatcctatt tctttaaagc gtaaatctgc tcgttttttt
    22981 tctggaagct cagttagtga gtgaccgttg atcactattt ctccggaagt cggtgtttgc
    23041 aatcctcctg aaatggtaag gaaagtactt ttacctgaac cagaaggacc aatgatgctg
    23101 acgaattctc cttcctttac ataaaaatca attgctttca aagctgagac ctttgtatga
    23161 ccagaaccaa atgttttggt aacttttttc atcgttaata tttttttcat ttttattaac
    23221 ctcctattgc tgtaattggg tctactttgg ttatcgtacg tatcgaaaac aatccgccta
    23281 gcattgcgac aatgatcaaa atcccactat atagcaacca ttgtgaccaa aaaacagcaa
    23341 aaggcatcgc gcttggtaaa atcagactag tcaaataagc aaacaaaaga gcaagcagaa
    23401 cgcctaccac cccaacaata aaggactgtg ctactaaaga tttagcgatg aagaaatttt
    23461 tgatcccttg tgctttcatt actccaaaaa tagcagtctt ctgaagtgtc atcacgtaca
    23521 tgaaaatacc gaccacggca gcaaccacta agaacaaaaa gtatatcatc gcatttaagg
    23581 tcatattttg ggcagaatag ccaggaatac tttcaatgaa tgtcccaata gttaataact
    23641 gtagaccctt cgatgctttt tctttcgaaa ttttagctga atcctttatt acgatcccat
    23701 tgatcggctg ttcttcttca gaagcaaacg gctgttgtcc atacttcaaa gcagtccacg
    23761 tatctaggga cgcatagatg acgggagaaa ctgtgtaata agtttcagga aagataccga
    23821 caatcgtcaa aggatcgtcg taactgccaa ttttgatcgt atctccaatt ttatagcctg
    23881 aatcagccag attttgagag atgatgattt cattctttgt tttaaattca tgccctttcg
    23941 tgagttttgg taataaaaat gcttctttcg ttgtgccgaa aactgtaaca ttttctttgt
    24001 tttttccttt gattgctccg ctgtacaatc cgattggtgc tttctctcct ccttcaatgt
    24061 atttcagatc tcctcttgtt aattgtgaag ctgcaaatac ttgatttgca tcctccgaca
    24121 aaacaatctc ttgtgcgtcc caatcatcga ttgctttttt aaactcttct gaaagtccat
    24181 tcgctagtcc tgacaacatg aatacgacat acgcaatcaa gaacataata ccgacgatca
    24241 agccatatcg taatttagaa tagcgaattt ctttgatagc tagaaacatt tgtttttcct
    24301 ccctatgata gattgatagt gacaaaatgt cacttgttga atagtgtaac tctaaagtga
    24361 caatttgtca cttgtgttgt ttttttctta tcaataaatt gtctaagaca tctttcgata
    24421 aaaccttaac atgagtcata aaaaatttat ttaagcctta tttatgcttt ccatgataaa
    24481 ctaaagtgac caaatcaatg aggtgggaaa atgattaaac tattagtagt agaagacgat
    24541 aaagtattat ccaataatat aaaagaaata ctcgctgcaa tcggcgagat aacacaagtt
    24601 tatgatggta gtgaaggact gttcgagttg gaaagtggag tttatgatct tgcaatcttg
    24661 gatttgatga tgccggaaat gaatggatat gaagtgttag caaaaatacg gaaagaaaaa
    24721 atacagactc cagtattgat tttgactgca aaagatagtt tggaagataa gattgaagga
    24781 tttgaacgag gtgcagatga ttatctaaca aaacctttct atcgagaaga actgatcatg
    24841 cgcgtgaaag cattattgaa acgctcgcta ggtctctttg atgaaaatgt tctgaagtat
    24901 aaggatatca gttgtgattt aaaaagcaat gaagtcttgt ataaagaaca agttttgccc
    24961 atacaaggaa aagagtttga attgctcgtt tattttttac aaaataaggg aatcattttg
    25021 acaaaagagc agatatttga ccggatctgg ggatttgatt ctgatacgac gattacagtt
    25081 attgaagttt atatgagcaa cttacgaaaa catctgcgaa gtactggatt ggataaagaa
    25141 atcaaaacct tacgtaatgt cggttatata ctgcaaaggg tgtgatgcaa tgaatggaga
    25201 tattacacga aagcaacgta gtcgcttttt tgcgacaaat gtagcagcat ttgcttttat
    25261 cttttttctt ttgggattca ttactttaca gatattaaac caatctgcat acagagaaac
    25321 agatctgtca ttagagaaaa taaattttga ttctcgaaca gtacaattag agatacaaag
    25381 atatcagcaa ggcgatccct ttttaaaccc gactcaaacg gaacaaaaca gaacaaacat
    25441 ggaacagcca ttaatggaac catcaaatga ccgttttaac actcaagtga tcttgtggtc
    25501 taaaaatggt gagatattga ataaagcagc tattggtggg cgtttcaacc aaatcaaaga
    25561 attaaaaatg gatacgaaaa atctgaatac catccaaaat attgaattaa atgaagacag
    25621 tggctcatct gccctctctt tccgttcgat tacgaaaaaa gcggagacga atcaagatgg
    25681 cgttgcctac atccaaattt tagaaaatac aaatcaaatc aagaattcat tagcgacttt
    25741 tcgcacgatt atgattttgt gtatgattgt tttttggatt ctctctattg gtatcagtta
    25801 ttacttgtct agtctaagca tgcgccctat tattctttct tggagaagac aaaaagaatt
    25861 tgttgagaat gcttctcatg aattgcgcac accgttaacg attattcaaa atagtttgga
    25921 acatcttttt accaagccta atcatactat tattgaagaa tccgaaagca tcgctcaagc
    25981 attgtcagag acgaggcgcc tcactggatt gacttcggat ttgctgacaa tcgctcggaa
    26041 tgattctgat cagcagctgc tttcaaagca aatgatcaat acacaagaat atatcgaaca
    26101 acttgttaaa ccatttcaag agatggccat catagatgga aaagagttta ttctccataa
    26161 cgatgcaaca ttagaagtac caattgatga aaaaaaaatc catcaagttt tggttatttt
    26221 gctggataat gcattaaaat atacgaatcc agaagataag atcattttgg attcatctgt
    26281 taaccataaa gaatggctga ttcacgtaaa gaatactggc ccttctatca gtgaagaaga
    26341 tcaagcaagg atttttgatc gattttatcg ggaagatcaa tctcgggcga aagagactgg
    26401 cggatacggg cttggcttgg caattgcaaa acaggtaatc gaacagcatc atggacagat
    26461 ttctgtatcc aattatcttc cacaaggagt cgattttaaa gtccaattgc cgttgaagcc
    26521 taaaaaataa tgcttatccc taaaaaaggg ctgtgacaaa aatcttgtca cagccctttt
    26581 ttgttgcgaa taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcttt
    26641 aagctgacca cttctgtcac aacctcttta gcttacgctt acgatttttt attgcggtct
    26701 tcaatgtgta catcaccgct tgtgtcaata tccaattctt ctcggctaac atcttctgtc
    26761 actttttcaa catcttcatg ttcttcttta tgaatcccaa cttcttcacg aataactgta
    26821 tgtttgctta catctacttg ctcttctttg attgggatag taatcgtgtc atcttccaat
    26881 ttttcgtctg ttccctgtga agattgatct gttactggtt tgcgttcgat gacgatttct
    26941 tcacgtttga ccggtacttc taccgtttca gtatcgttga ccacatgttt gtggatgtcg
    27001 acttctccag ttgtcacatc atgtgtattt acatctaatt tttcttcttt tagttgaatt
    27061 gtatcatcat ctagatttgg tttgcgatcg atgtcttcag tcttgcttgt acccattgta
    27121 tcagtctcgc caggtacgtc catcggatca gttcctggtg tcatattcaa tggatccgtt
    27181 gtattttgaa agcctgtcgt gccacctacg tccattggat ctgttccagt gccagtttct
    27241 aatccagtcc ccattgtgct tgctgggcta acagtatctt gtgtatccat tgtatcttct
    27301 gttgtttctg gctgacgata gccttttact gtgatgacaa attttccatc agaaatatct
    27361 cctctatatt gatataaagg atcattttct ggtgtagccg tctcagaatc ataggtatca
    27421 aaagaaaaag tatcttttat tttttcccat aaagaacggt cttcttcacc atccatccga
    27481 ttttcatctg tttcaacatc tataccagac aatccgtcaa atcctagtct agatgctgct
    27541 tcatcagtag tataaataac gatatcatct cgttcatacc cttcgttacg caaccgttca
    27601 attactgata atgcttcttc cctcgtacta tagcttccga tgaccgttaa atttttctct
    27661 tccatgtaaa ttcctcctag caaatagatt tatgattggt atattgttac ttataaattt
    27721 attctatagg agacgattgt tttctacaaa tatttagatt ggaatttgat taaaa
//
LOCUS       DAYYHX010000014        26819 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000014 DAYYHX010000000
VERSION     DAYYHX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.014"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..485
                     /locus_tag="ACHA6Y_000442"
     CDS             <1..485
                     /locus_tag="ACHA6Y_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8986561.1"
                     /translation="FNKNTVRLKYALDKYQRKTHTKGWKDTVIQAPEVVMTLLREWKS
                     AQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRINAIKKRHPELAQLSPH
                     KLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVVDKSVFEAFQKGLNKCD
                     "
     gene            complement(574..966)
                     /gene="rpsI"
                     /locus_tag="ACHA6Y_000443"
     CDS             complement(574..966)
                     /gene="rpsI"
                     /locus_tag="ACHA6Y_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8986562.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(982..1410)
                     /gene="rplM"
                     /locus_tag="ACHA6Y_000444"
     CDS             complement(982..1410)
                     /gene="rplM"
                     /locus_tag="ACHA6Y_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM8986563.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(1944..2411)
                     /locus_tag="ACHA6Y_000445"
     CDS             complement(1944..2411)
                     /locus_tag="ACHA6Y_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8986564.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(2531..4069)
                     /locus_tag="ACHA6Y_000446"
     CDS             complement(2531..4069)
                     /locus_tag="ACHA6Y_000446"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM8986565.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            4149..4835
                     /locus_tag="ACHA6Y_000447"
     CDS             4149..4835
                     /locus_tag="ACHA6Y_000447"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM8986566.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5036..5563)
                     /gene="msrA"
                     /locus_tag="ACHA6Y_000448"
     CDS             complement(5036..5563)
                     /gene="msrA"
                     /locus_tag="ACHA6Y_000448"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8986567.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(5647..6324)
                     /gene="pgmB"
                     /locus_tag="ACHA6Y_000449"
     CDS             complement(5647..6324)
                     /gene="pgmB"
                     /locus_tag="ACHA6Y_000449"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8986568.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(6321..7449)
                     /locus_tag="ACHA6Y_000450"
                     /pseudo
     CDS             complement(6321..7449)
                     /locus_tag="ACHA6Y_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(7449..9632)
                     /locus_tag="ACHA6Y_000451"
     CDS             complement(7449..9632)
                     /locus_tag="ACHA6Y_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM8986569.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(9629..10654)
                     /locus_tag="ACHA6Y_000452"
     CDS             complement(9629..10654)
                     /locus_tag="ACHA6Y_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8986570.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(10699..11526)
                     /locus_tag="ACHA6Y_000453"
     CDS             complement(10699..11526)
                     /locus_tag="ACHA6Y_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8986571.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(11523..12590)
                     /locus_tag="ACHA6Y_000454"
     CDS             complement(11523..12590)
                     /locus_tag="ACHA6Y_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM8986572.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(12616..13452)
                     /locus_tag="ACHA6Y_000455"
     CDS             complement(12616..13452)
                     /locus_tag="ACHA6Y_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8986573.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(13449..14318)
                     /locus_tag="ACHA6Y_000456"
     CDS             complement(13449..14318)
                     /locus_tag="ACHA6Y_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8986574.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(14388..15671)
                     /locus_tag="ACHA6Y_000457"
     CDS             complement(14388..15671)
                     /locus_tag="ACHA6Y_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8986575.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(15685..17370)
                     /locus_tag="ACHA6Y_000458"
     CDS             complement(15685..17370)
                     /locus_tag="ACHA6Y_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8986576.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            17645..18670
                     /locus_tag="ACHA6Y_000459"
     CDS             17645..18670
                     /locus_tag="ACHA6Y_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8986577.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(19265..22918)
                     /gene="rpoC"
                     /locus_tag="ACHA6Y_000460"
     CDS             complement(19265..22918)
                     /gene="rpoC"
                     /locus_tag="ACHA6Y_000460"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM8986578.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(22997..26614)
                     /gene="rpoB"
                     /locus_tag="ACHA6Y_000461"
     CDS             complement(22997..26614)
                     /gene="rpoB"
                     /locus_tag="ACHA6Y_000461"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8986579.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
BASE COUNT         7353 a         5830 c         4991 g         8645 t
ORIGIN      
        1 attttaataa aaatacggtt cgtctcaaat atgctttaga taaatatcaa agaaagacac
       61 atacaaaagg ttggaaagac accgttattc aggcaccaga agttgtgatg acattattaa
      121 gagaatggaa atcagctcag gctgaacaat tgttgaaact aaaaattata caaacacctg
      181 accagtacct ctttacctat tccaaacctt ctggagaagt aaattgtcct gttcacgctg
      241 actacttaaa ctatcgaatc aatgcaataa aaaaaaggca tcctgaattg gctcaattat
      301 ctccacacaa acttcgtcat acctatgcaa ctatagctag ggaaggtgga gcaaatatga
      361 atcaaatctc taatgcttta acgcactcgg atatttctac tacaaaaata tacgtgaaca
      421 cacctgacgt agttgataaa tctgtttttg aagcatttca aaaaggattg aataagtgtg
      481 attaacatgt gttctcaaat gtgttcttat tataaaaaaa gacactttcc aaataattga
      541 aaagtgtcct gaaatattga tataaaaaga atattaacgt tttgagaatt gtgaagcttt
      601 acgagctttt ttaagacctg gttttttacg ttcgaccata cgtgcgtcac gagtaagtaa
      661 gccagcacgt ttaagagcta aacggaagtc aggatcaact tctagcaatg cacgagcgat
      721 accatgacgg atagctcctg attgtccagc gtatcctccg ccgtttacgt taacaaatac
      781 gtcatatgcg ccttttgttt cagtaacagc gaaaggttga ttgattactt cacgcaagtc
      841 agcatgtgga atgtattctt caacatcttt tttatttaca gtaattttgc cagttcctgg
      901 tactagacgt acgcgagcaa ctgcgttttt acgacggcct gtgccgatat attgaacttg
      961 tgccaatgaa attccctcct attagattaa gtttgtgatg tctaaaactt ctggttgttg
     1021 tgctgcatgt ggatgttctg ctccaccgta tacgttcaat tttgtaaatt gtttgcggcc
     1081 taaagtgttt ttaggaagca taccttttac agaagtttcg atcaaacgac gagaattttt
     1141 agcacgcaat tcgccagcag tgattgattt caatcctcct gggtagttgc tgtgacggta
     1201 ataaattttg tcagtcgctt ttttacctgt taatttcact ttgtctgcat tgatgacgat
     1261 gacgaaatcg ccagtatcca aatgaggtgt gaaagttggt ttatttttac cacgtagtac
     1321 agatgcaaca actgttgaaa gacgtcccaa tggaacatca gttgcgtcta ctacatacca
     1381 tttacgttct acttcgcctg gtttggccat atatgttgta cgcacgttat tttcctccaa
     1441 tttaagttct gatgtttgac atacacttcg agtttccggg gctctttgtg gggcaaacaa
     1501 taccatttaa catattaccc gctaatctag tcaatgtcaa cgtttttctg cttgattgaa
     1561 aaatttatat gaaactttac ttcttttctt tcttttatat aaaagggtcg tttcctgtca
     1621 aaatgaaaaa caaaaagagg ctgtgacaaa agtcttgtca caacccctta ttttgaataa
     1681 acggtgttct cctgccaact cctcggtcac aagtcacaat atccataaaa cataagaagc
     1741 tgttttttaa aattgtttct tgtgactcgg agctgaacgg caggcttaca acctcttggt
     1801 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
     1861 ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctttt
     1921 tcggtcaagt tcaacaacag tttttacgcg tcgatctctg gcgctttgcc caaatgagct
     1981 tggatcatcc agatacgttt ttctgtggct gttttgaatt cgataaagat atcttgtgtt
     2041 ggatcgtcgc cttcttctcc agaaacttcg attccttttt ggtaaagatc tgctagataa
     2101 cgatagcctg caacaagtgt ttccaaacgt tctggaatag tacgatccca gcggccaatt
     2161 tcatcaggga tttttgtgtt atctgcaaat tcacgcaatg tagaataagg tgaaccgtct
     2221 aatgtgatca agcgttcgga tacttcatct aactgagcgt tcaaatcatc catgaactca
     2281 tccatcattg ggtgcaatgt aaggaaacct ggtcctctca tgtaccaatg tgtttggtga
     2341 acaacaacag acatttggct tagatctgca actaattgat ttaatacttc tttggtttgt
     2401 tggaatttca tctttcttgc ctcctaacat tttcttacac ttaaaataat agtagacttt
     2461 caatgaaaag tacaacaaaa tcatttacct gagaacgatt ttcagttcgc ttttttatcc
     2521 gtaaagccaa tcatttttca gaatcagcta attcttctcg cagttgagca aattgctgat
     2581 aagtctgtgc taaatgcttt tgtgtcgaaa caaataatgg atagattttc tgatattttt
     2641 tatgatcagt ttcttgtact ccaacttgct ctttcaattc tatcaattcc tgtgcttcac
     2701 tgatactttg gatctttccg atgcttctcc agcctaaaat cacagctcct aatgcggatg
     2761 cttctgtgac agtagggaaa acaagcgggt aacccagtac atcagccatc atctgacgaa
     2821 aaatcggaga ttgagcaaac ccgcctgtag cacggatctc tgtgagctga ttttcctggc
     2881 aaactccttt taaaatccga taaaggttga gacagatccc ttctagaatc gccttcatca
     2941 tttctttttc tgtatgttgc cgctgtaaac cgataaaact gccaaacgca tctgcgtccc
     3001 aaagcggtgc tcgctcacca agtaaatagg gatgaaaaat cagaccattg gcacccgctg
     3061 gcacaaaagc gatagccgaa agcagtgcat catatggatt ttctcctttt tctttagcag
     3121 attgaacgac ttcctgtaaa aatgtatgcc ctgcccaatc aaagatgcca gcaccattgc
     3181 tagtcgctcc tcccactacc caatgtttct gatccagaac ataacaaaac gtctccattt
     3241 ctggatggaa ctgaggctga gtagtaataa aacgaagtgc tccgctcgtt cctacggtca
     3301 aagcggcgat cgtatcacca aaagcaccta accctagatt agaaagcggc ccgtctgccc
     3361 ctcccaaaac aaatgatata tcatttggca aacctagttc tgctgctgtt tttgatggta
     3421 gacctgttac ttgataggtg gaaggaaaga cttgcggcaa ttgctctatt cgaatgttca
     3481 agtagtcaag cacttcttct gcccattgga attcatggat atcaaaatat ccagttcctg
     3541 aagctgttcc gtagtcgctg atcagttcgc caaacaatct ataaaagata tagtctttga
     3601 taccgacaaa ataagcagtt tttttgaata attcaggttg gctgttttgc agccacttga
     3661 ttttgttcaa tggtgtcatt ggatgaatcg gcgtcccagt ctgccgataa aattctttgc
     3721 caagcacggt atatttcagt ttttctgctt cgtccgctgc tcggttatct gcccaagtga
     3781 tcattcgaga taacggtgtt ttctcttcat ccaacaatat caggccatgc atcgcagagg
     3841 agaaggagac aaattgaatg attgtgtctg aaggcaactg tttaattact tcttttataa
     3901 cggtgcagac accttgaaag atcaattcct gatcttgttc tgccatccct tgtgtttctt
     3961 ggatcaaagg gtagcgaaaa taagcggatg ctgcttcttg ggcttcatct gtgaaagcaa
     4021 ctgctttggt ttgtgtggtt ccgatatcta atccgattga aacgttcatt ttttcacctc
     4081 aaattttatg tatgtacaaa aatttagttt aagcttgcgt atcttggttt tgaaaagggg
     4141 gagatggcgt gttactttat tttatcattg gttgctgtat cggatcattt ttatgtttaa
     4201 ccgcccagcg gattccgtta ggtcactcta ttatttatcc tagatctcat tgtgtcaaat
     4261 gctgtcgttc gctttcttgg tatgaattga ttccgattct ctcgatcatc gtccaacgat
     4321 ttcgctgccg ctattgcagg tgtcggttgc ccttctacta tttgttggca gaagccttat
     4381 gtggtggtct ttttgcctgg ttctttactt tttcttccgc aagaaatttt tcgacgttca
     4441 tatggatgct ttcggctttg ctattttcgt tgatggattt attttacttc atggtccatt
     4501 ctcacacgct attctgttct tgggcaattt tatggatttt ttggttgcag gccggcgtct
     4561 tccaatggca aagtgtgctg ttaacgttta tcgtaggcgg tgtgtgcctt cgctatggtc
     4621 aatcctatct cggagctggc gatactctgt tgatgctttc ttggagtggt ggcttgtctc
     4681 tagaagagtt gcttcagatc ttgtttcttg ctagtctgtt tggaatatcc gttttttctg
     4741 tttactttat ctgtcataaa aaaaagttgg aaaagctgcc tttcattcct tttttgagca
     4801 gtgcgttgct gattgtcctt catcttaggt attgaaaaaa ggttgtgaca aacgacttgt
     4861 cacaacctct gatatatagt gttctcttac caaatccttg tgacttggag ctaggcgaca
     4921 ggttcacaac ctctccacta aatggtgttc aatcagctga ccctcagtat tagctcaaaa
     4981 tgctggtttt acaacctctg atatacggtg ttccataagt agctttttcg gcttcttatt
     5041 tcttgacgcc ccaatgtcga cgaaggaaac ctgcacgtcc agaaccttct cgataaagat
     5101 tgtaatggag cttgtttttc ttgtaataat cctgatggta atcttctgca ggataaaatt
     5161 cgcttcttgg ttcgattttt gtcacgattg gcttatcgaa tcgcccgctg tcttgcaatg
     5221 cttgttttga acgctctgca atatttcgtt cttcttcatc attatagtag atcactggac
     5281 gataagaatc accgcgatca gcgaactgtc ctaatgcgtc agtcggatcg gtttgctgcc
     5341 aatagatttc caccaactga tcataagaaa taacttctgg atcatattcg atctctactg
     5401 cttctgtatg ccctgttgtg cctgttgtta cttgttcata agttggattt ggtacatgcc
     5461 cgccagtata accggatgtc acagaaatga tccctggctg ctcttcaaat ggtttgacca
     5521 tacaccaaaa gcatccgcct gcgaaaactg ctttttttgt catataaaat cccccttttt
     5581 tctcatcata ccttgatttc caaaaggagg ataacgtttt gcttgtgaca gaaaaaacgg
     5641 aaactattat atacgtgata cggcttctga taaatgccaa aacgaacgta cttgatcaaa
     5701 aacggttaaa tcaggaatca catattcagc agaaggaaga tttatgtttt cacctattcc
     5761 caatacatgc atgttaccag ccaaagctgc ttgacaacca gcttctgaat cttcaatgac
     5821 caagcatgca tttggcggta cattcaattg ctgtgctcct ttgagaaaaa cttctggatc
     5881 aggttttgct tttgatactt gcgtgccgtc aataaggaca tcaaaataac ttgtcaaatt
     5941 cgttttttct aaaatgaggc gtgcattttt gcttgctgat cccaatccga tcttgatgtt
     6001 tgtttttttc aagtactcaa gaacagctat tgcaccaggc aaaacggatg tttcatccat
     6061 tttttgaatc gcttgaagat atagattgtt tttttgctca gcaaaggctg atttctcttt
     6121 ttctgagaat ttctgttcca tttttccaaa tgacagcaat cgctctagtg actcggttct
     6181 ggatattcct ttcagttgct cgttttcttt ctcagtgaat ggaatggaca gctggttagc
     6241 taaaaccagc cacgcttcgt aatgataacg agctgtgtct acaagaacgc catctaaatc
     6301 gaatagtgct gctttgattt tcatcgatta cctccttgaa aatgaacgac ttgaagtaaa
     6361 gatgcgatgt ttttagctgt gcgatgaaga ttatcagtgg cttgctgtaa agcttcttgt
     6421 aaaggcacag ctgcaggaag gatcggaaaa acggccgaaa tgccaacatc tgctgctttt
     6481 tcgcttcctt taccaacact tccgcaaata gctatcacag gaacgccctc tggtactctt
     6541 ctagcaatcc caactggcgc ttttcctttt agactttgtc catctagacg tccttcgcct
     6601 acgattacga ggtctgcttg ctgtacccgt atcgaactcc agtaaatcca atacgtagtc
     6661 gatgccagag actattttcc ctgaagcaaa tgctaacagt cctgcaccca ttccaccacc
     6721 agcactactg cccgatgcct cgattacttg aggggcaaat ctcatataaa aatcatgcat
     6781 ggcttggtca gccactgcta attcattttc tttcaagcct ttttgtggtc cgaaaacaaa
     6841 agaagcgcct tcttttccgc ataaaagatt agtaacatca gtaatgacag tgattttggc
     6901 tttaatcagc tcaataggca cgttcgtttt atcaactgta gcaattttac ctagattttc
     6961 tcccagtgct tctacttttt gattatcttg atcgtaaaaa gcataaccta gaccttctgc
     7021 catgccgatg ccgccatcgt tggtactact tccgccaacg ccgatgatga tgtgtcgaac
     7081 accttgttgc aacaattgtc gtatcagttc tcctactcct cgcgtggata acctaagtgg
     7141 ctgttgttgg tttagtggga catctttcaa tccgacgatt tctgccattt caaaaatcgc
     7201 agtttgaccg tctgaagcgt actggatttc tttgtttttg ccaaatgcat tagtaacgac
     7261 tgctgtttgc cgcttcaacc ttaaactgtc tgctaatgat gaaaccgttc cttctcctcc
     7321 atcgcctata ggtagcagat catattcagc ctgaacaaaa acttcttgaa aacctgattg
     7381 aatggcttgt gctgcctcta aggctgtcag actttctttg aaagaatctg gcgcaatcag
     7441 aatacgcatt attttcccct ccgttccaag atcagttctt tttccagctg gtattggtct
     7501 ccattgacaa aaataggtaa tgtttctttc gtctcttttt ttattactat ttgttttttt
     7561 gtcaaaacaa atagcaacct gtgatttttc caaataaaag gaaaagaaat tttttcccat
     7621 gctgaaggaa gtcttggaga aatagaaaga gttccattcg ttactgtaat acctgaaaaa
     7681 ccaaatataa ctgctagcca tagtgctcct aatgaagcag catgaattcc gtcatctgat
     7741 gaatgcggat ctggtcctaa atcaatcagg cacgcttctt ggaataaatg atacgcccat
     7801 tccatttctt ggatacggca agcctctatg gcgtgaatcg ctttactcaa cgatgagtcg
     7861 tggattgttt tgttttcata gtaatgcaaa ttcttagaaa cgattggttg agaaaaatac
     7921 tcaggtaata agtataacag catgacaaga tctgcttgct tgctgacttg gagttcattt
     7981 acttcgctac gggcataatc aagtaaaata gactggctgc caggacgttc tttgtaagtg
     8041 gtcaaatcaa taactggctt actgagatac gtgtcatctt gcggaatcag ctgttctttg
     8101 ttaggcactg gcaaatataa cttatctaaa aaattttgac attctttcaa aaaaagcgga
     8161 tcgccagctt gatatttaac agctaaggcc acattataat gcgccagata gttcgtatat
     8221 gcattattgt cgatatattc tgtgtattca tctggaccaa tgactctgtg tatctctaaa
     8281 tgatcatcga cttttacagc tctgctcagc caaaaagctg ctgtttcatg aatgagcgtt
     8341 tttccctttg tttctaaaaa cgattgatcc tctgttgctt ggacatactc ttgaacggca
     8401 taagcaaggt ctgcactgat atgatgttct gcggcagctg aagcaacttc ttgtcttgtt
     8461 ccggttttga tattcaatgc agcaaatttg ggtgtctctt cctgaccagt gtgtgcgctt
     8521 tcccatggga aaagtgcacc ttgatagtga taatctcttg ctttttgccg tgcgccttct
     8581 aatcgatcat aacggtacag cagcaaattc tttgctactt caggaaactg atataaaaag
     8641 aaaggcagga taaaaatttc cgtatcccag aaaacatgcc ctttataacc ttcgccagtt
     8701 agtcccttcg cccctacact aacacttgga tcattgcggg gagtcataat ctgtaaatgg
     8761 taagcagcga agtccatcgc taactgatcg aaagaagaat cgccttggat cgttatccga
     8821 tgctgatgcc aaaaatctgt ccaataagac atcgactgct ctaaaatttc tgtataacta
     8881 acgcttttct gggcttgggc aatacttttc ttaaacgggt ctgtttgctg aagagaggta
     8941 gagagcacga tgatcttctc cagcgtaaag gacgtatttt ttctgatctt ttctgaaaag
     9001 gaagcggtga tccggcgatt ttttgcttgg aatacacctg ctttattgaa tttagctgca
     9061 atcccaatat caattttact ttctgttgtc tggtaggatg cactgatcaa atcgtcacca
     9121 aatactcgca gttctttttc aaccaactgc tgtgtaccaa aattggtttg ctgtccatcg
     9181 atacccgttt tcacatctac ttggcagtct tcggtcaacg gcttgatgac cactttcagg
     9241 gctgcctgat ggagattatt ttgatcagca acacgacaag taatgacttc cacttttttt
     9301 ccttgagcag tttcaatgag cgcatagcgt ttaagctctc ccatttctaa atctagtgcc
     9361 cgctcgtatg caagaacttt catcgtctca agagaaaaat gttcaccgtc gatttttatt
     9421 tcccatctga tgacatctgg gagattcact aactcggctg attctgctcc taaaggtcgg
     9481 ttatatacac cagctgcaaa cattcccctt acttgattcc gatagtcttc ctcctggctt
     9541 cctctgatac caaaaaatcc atttccaatg gtcatctgac tcgcatactt attgatatac
     9601 tctaaatcaa actgtttttc ttctactttc ataaggtaat aacttctcct gtttctgccg
     9661 aacgatataa attttccacc agattctgga tgacatatcc ttgccagcca tctgcaatca
     9721 tctgagtctg gtcaccgagc gcccgattca caaaatgagc catacttagt tcatgtgggt
     9781 cattaactgg cgtggtcttt tgtaaggaaa ccagctttcc atctacatct tcgaatagtt
     9841 cgcctggata cagattagca cctgcctgat cgccacaaaa agccacattc agtttgtctt
     9901 ctctgatatt caaagcaaac gaagtattca gttggatgat cttgccgttt tctaattcca
     9961 gtgtgccaaa aattgcatct tctactgtga atttgcttgg atcccatttc ccgaatttcc
    10021 ctgaattttt tttcgtacca attttttgga agctgtacgc ccgaacgctt tttactttag
    10081 gaaatccagt aatatacatc gctgtatcaa gcatgtgaat gccataatcg atcaaaggcc
    10141 cgcctccttg aagttctttg tcgataaacg ttccccagcc tggaacacca gaccgacgaa
    10201 gcgcattcgc ttctacgtaa taaatatccc cgagaacatc aagatgattc ttgatcattg
    10261 ttgcttcgtc agaaaaacga tgctggaatc cataagccaa aatctgcccg tttttatcgg
    10321 ctgtctctgc catctttctt gcttgatcag ctgtcatagc tggtggtttc tcgcacatca
    10381 ctgaagcatt agcttcgagg gcagccaata catgttcgaa gtgaaagcga ttggctgtac
    10441 agacactcac gatatcggga gaaatctcat tcagcatttg tgtaactgat gtatacgcat
    10501 gagaaaatcc tgtacgtcgg caaaagtctt ctgctcgctg gctgactgga tcaaccacag
    10561 cggccaattc taagacatct gatctttttt gataataagc agcatgtact ttttctgcta
    10621 cttgtccagc gcctatgatt gctactttct tcatttattt ctctctcctt tgcttattta
    10681 cttattgtta ttctttctct attctttctc taaatcattt aaatgacgcg ctgtttccag
    10741 acagtttttc aagtattgca atgattcctg ataaattttc acttcatttt ctcctcgcaa
    10801 acgacattca tataccatat aaccttcata ctcattttga agtagcgttt gaaaatggcg
    10861 agtaaaatca attgcgccag atcctggttg gtagcgatga ttatctgcta aatggatatg
    10921 acccacatac tctttttgcg tagtcaatgc ttttgaaata tcatcttctt caatattcat
    10981 atgataaaaa tcagcgatga tctgcacatg tttcaattga ttagagtcaa tgtaatgacg
    11041 cgcatccgct aacaaattga tcatatggtc ttggtatcgg ttcaaaggct ccaaataaat
    11101 aaccgtacct gtttcttcgg ctactttatc taaataacga agtgattcgc tgactgcttt
    11161 atagtctcct tctttgcttc ggggtgaaac catcggcggt aatcgataag tgaacattcc
    11221 ccaagctgct gggataacga ttcctttgcc tccaacctcg cttagtgcac gcaaaatctc
    11281 agcaatttga cgtacaccat taagacgccg ttcttcgata aagtcaccga tccacccatc
    11341 gtagccgtta caagctgtca ctactgggat ccctgtttct ttgattgcct gtttgactgt
    11401 gtcgatattt tctattaaca acttaccgtc gatttcgtat tcttcaaatc cacagtcttt
    11461 gataaatcta aatttttcta aaatgtctct tgggaaaaat gcttgatctt gcgtaccgaa
    11521 tttcattgtt ctcctcctcg gacttgaacg cccattttga tactttcttc tgggtgctgg
    11581 tctacataac gcatgtaagc ttctgcgctt tctgcaaatg gcacaactgg atcaatgatt
    11641 tcttcacaat ccaagtaacc gttcatcaat aattcccaac atgtgtcctc gattcgttta
    11701 cggttccatc ttgggtaatc tgggtttggt tcactagatg ctcgagcaaa gacgattttt
    11761 gcattattga aatgtgcttc gcgaccaaga ttaaacccag ctggaaaagg tttagcaaag
    11821 gcaacataag aaatgatccc gccatagccg atccctctta aagaagcttg taatgcggca
    11881 gcactgccac tcgtttcaat aactgaatct actccacgtt tatctgtcaa ttcctttaga
    11941 cgaaaaccga catctgcttt cataggatct agcgtgtaat cggcaccgta tttttctgct
    12001 aattctctac gctgcgctat gggatcgatc ccaacgatgg tagtagcacc tgctttttta
    12061 gctaattgaa tcgcaatttg cccgatagcg cctaaaccga agattgctac agcatcacct
    12121 gctcgaacat gtgcttcccg gataccacct aaagcgaatt gagcaggatc gtaacatact
    12181 gcatttttcc attgatccaa ccgatcaagt ttacgtagtt tataattatc aacaccgttt
    12241 gtaatcagcg tttcacgaat gggtccataa gtgcagacta actctccttt ttgatactct
    12301 gtcaccaaac tgcccgtttc ttcgatcgta ccgacaatca tatttcctaa ttgaaattcg
    12361 ccaaatacca caccgcgttg gcttccttct tctcttggca agaataggcg ccattcttct
    12421 gaaaattctt catcgataaa tggacttaat cctctaaagt cgacaatttc tgttcctggt
    12481 tttggagtag cgaaaattac tcggatacgt acttcgttct tttcgatctc acggtcttcg
    12541 taatctttga atccggctat tcttggttct aatgcaacta attttttcat cttttctttc
    12601 ctctttcttt tctttttatc cttttacgcc gccttcgact aaatcacctt taataaatcg
    12661 ttcagataac gcatacataa tgatgactgg taaagcggtc accaatgaag cggccatcat
    12721 tcgtccccaa atgtagtctg gtgtactgaa taatgtgttc aaaccgattg gtaaagtaaa
    12781 tttttcggag gtcgacagga aaatcgaagc aaataaataa tcattccacg aaaccataaa
    12841 gcaataaacg aagacagaaa tgattccaga aatagccaat ggtataatga tccgaataat
    12901 gatctggatc cggctaagac catcgatcat tgctgcttct tcaatatcag tcggaatcgt
    12961 gtcaaaataa ctttttagca tgaatatcgc agtaggaagt gtttgaacga ccatcgtgat
    13021 aatcagtgct gtattggtat catacaaacc tagaccggaa atgattttaa ataatggaac
    13081 aacgatcaaa ataccggaaa acatataaac ggtgtaaaag ctagcattga ttttcgagcg
    13141 agctttgaag cgtagttttg acaaagcgta tgccccgctg atcccaatca ctactgcagt
    13201 tacggaggct atcaaggaga tcactaaact atttttgaaa tacatgatga atggaaaaat
    13261 ctcagcattg aaaatatcct gataatgttc aaaagtccat tctttcggaa aaatagtttg
    13321 tgtcgttgca attgcttctt gtgaatcctt gaatgatgtc atcagcatga tcaaaaatgg
    13381 aaacaaattg acagctaata gcaacaaaat cgtcgcgtaa aataagattt tcctgctctt
    13441 tttatttttc atttcaagtc caccttcttt cgtgagagca agacgaccgc tgcaataatg
    13501 atgaacaata gaatcgatat agctgctgct tttccaagat cattgaacgc aaaggctgtc
    13561 tgatacaaat aaacgcctaa gatattgact ttattcgtca gtagatagac atctgtaaac
    13621 atatagaaca tccagataga tcgtaaaaca atcacagttg ctagtactgg tttgattgcc
    13681 ggcagtgtca cgatcttgaa cttctgccaa gcatttgcac cgtccatttc agctgcttca
    13741 taaagcgttt tgtcgactgt ttgcaaaata gccaaaaatg aaataaacgc atacggatag
    13801 tatctccaga tagcaaacaa aacgaccata aagaagctag caactggctg gtcgaaccac
    13861 agcggtgctt cgttgaaaag ctgcaaccta tctactaaca gatcattgac gattccataa
    13921 ctattattga acatatattt ccaagcaaaa atcaaagaaa tagatggcgt aacataactt
    13981 aataaaatca gtgaacgcac cgttttccta aatctgaact tgcggttaag caaaatagct
    14041 acggctaatc ctaaagctgt actgcctgct acaactaaga atgtgtaagc aactgttaaa
    14101 cctaaagaac gataaaattc actatcactt aaaatttcaa gataattgct taatccgata
    14161 aaaacggcgt ctgagttagg attcagcggc atatcgaaaa aactgatctg aatattggaa
    14221 agcattggaa tcagcactaa aacagctaat aaaagcatgc ttggtgctaa aagtcccatg
    14281 gctaatttta agtcagaatg tttccttgga cgattcattg aaacctcccc ctgataatct
    14341 gccaatagct atctactttt ttcgaacacc attggcagaa cttcttgtta tttcgtattt
    14401 ttttgtgcgt cagctaaagc ttgatcgaca tcttcgtttc cgacagttac ttggttcact
    14461 gcttttccga tcacacctga gcttgtgata tctcccattt tcgtgaaatt cttttccccg
    14521 actagtccaa agacttgtac ttcatcaaaa gcactagcaa tctcagatgg cagctcgcca
    14581 aaagacttga tgacttcatt ttctttgtaa ccatccagtt cgactacttg tttattcact
    14641 ggttgagcac caccaggaga catcaagacc cacttttcca tgtttttcgg ttgagataag
    14701 aaagctgtga atgcttcagc agcctctttt tgtttctcgt ctagaccaga agaaatcgtc
    14761 aatccagata ctgtgccata gacagcttga tctttttgtg ttgggatagc aaaaccgata
    14821 ttttctgagt tccccgcttc ataaacagaa ggcaaaatgt aagttgaata gattgccatt
    14881 ggtgctgttc cattcatgaa agcatctttg atttctgtca catcattaga gcctggcatc
    14941 gtgtattggg ataagttttg ataaaacgac aatgcttctc tcatcttttc cgtatcgatc
    15001 gttacctcgc cttcatcatc caaaacattt gctccatttg acaaagcaaa ttgagagaac
    15061 gcttgctcac tcattgttcc ttcagcagta ggcatagcaa ttccatattg tttattctct
    15121 ttatctgtga aatgttgagc aatttttaaa atatcatccc aattttctgg ttctgaaaaa
    15181 ccagcttctg ataatttttc tttgtcatac caaatacctt gaacccagcc gctaattggt
    15241 gcagcaatat agctttttcc atcttctgaa cgaacaaggt ttttggctcc gtcataataa
    15301 ttatcttctc cagcttcctc gataacgttt tggactgccg tctgatcaat cagttcatct
    15361 ttatccatca ctttagcaaa atcctgactg acttccatta cttctggtaa tttaccggaa
    15421 cgagctaacg taacgatttt tgtattgaac gctgattctt ctaccggcac ttgcttgatt
    15481 ttgatgtctg gattttcttt ttcaaaatca gcaaccagtt cattgatcac acttaatcgt
    15541 tcctgttcga cagaagagtg catgaattga atcgttgtct gttgtgattc gttagagctt
    15601 tgactttcac cacaagcagt aagaagaaat ccagtagcta gcagacaggt agtaacaata
    15661 atttttttca tttttttcca cctcttattt tattattttc agccacatat attcatacgg
    15721 ctggaatatc aactttcgtt cttgactgca atggataatt tgtccagaaa acaggtctag
    15781 gtaatctcct tttggtaaaa gataacctgc tgattgattg gaaaaattat ggataactaa
    15841 gactttctct tccttcgctt cgcgaataaa actgataaaa gaatctgctg agtccagtaa
    15901 gtaaatcaaa ctattcggat ggaaggcttt ttcttttttt cgaatagata gcatctgggt
    15961 caattcacca tatactgctg ttcttaatgg atctgtctgc aactctttct caatatctgc
    16021 taacaaaaat ttttgccgat tgatttttcg attctctcct gttttttcta cgcctttgta
    16081 atcatttcga cttcccagca cactttgaat ataaattgcc ggcacgcctg gtaacgttac
    16141 caaaaatgaa tgagcagcta aaaagcgttt gcttctccat tccgtcgtat ctgttggaga
    16201 acttaacgca tctagatacg tagcgtttat ctcatatgga ctggtcgttc catctgggtt
    16261 tttcttgtag tttactcggg caccctcttt cttcagctgt tccactaatt caaggatcgt
    16321 tttttccggc agaatacctc gtaccggatt caatccgata ccgtcatggg aggctaaaaa
    16381 attaaaaaaa gttgtttttt tcgatgcgaa atcgatggtt tttaaccatt ctttcaactt
    16441 acttgtatta cccgaacgaa tcgcttgaag ggtcaaaggt ggtagcgaaa actgatagac
    16501 catctgtgct tcattctcgc cattcccaaa ataactgata ttatctttgt gtggcacatt
    16561 tgtctcagtg atcaataccg ttcctggagc tgcttcatca gcgattgcac ggaataattg
    16621 gatgatttta tgcgtttctg gcaggtgtat acaagaagtt cctagttctt tccataaaaa
    16681 tcccacagcg tccaagcgaa taaatgaagc gcctttttct aagtaaaaca aaagaatatc
    16741 gatcattttc aataaaactt ctggattttt aaaattcaag tctacttgat cttcgctaaa
    16801 agtcgtccat acgtgttttt ctttcccgtt tttgtcaatg aacgttgtaa gtaacgggct
    16861 ggtccttggt ctagtcacgc tgcttgtatc tgtatccagt ggcaaatcaa taaaaaaatc
    16921 tttgtacgta tcattcaagt ttaaatattc ctgaaaccaa gtactttcgg cagacatatg
    16981 attgcagaca aagtcgaaca ttaaccgtgt ttcgcttctc atttcttcta tatcttccca
    17041 tgtgcccaaa cggtcgttga ttgcctgata atttacaacg gagaaaccat catcagatgt
    17101 atatgggaaa aatggcaaga aatgtacaac tctaaatgta tctgccaaat acttttgata
    17161 aaatttctta aatgtacgca aagttggttg ctgctcttca aaaaattgat ccccataagt
    17221 aatcaagaaa atatcttctt cgtcccaatg cagtttgcct gcctggcctt tcttctgctg
    17281 ataaacactg actttttgtt caatcataga ggcggttttc tcagacaaat ctgagtcgcg
    17341 ataaatcgct tgtaaaagtt ttgtatacat cggattcccc tttctttttt gggagcgttc
    17401 ccaacgattt gatcatatta tggaaaacgc ttactgtcaa tagcattttt ttaaataatt
    17461 ttgatataca caaaaataca aataagaaag cttatgtatc aataattaat agcttcttaa
    17521 aaaaatatgg tctctcatga tcatgcaagc tattcctaaa ttttcaaaat catagacaaa
    17581 aaagaatgtc tattgtacta tggaattatt ctaaaacgct cccaaaaagg aggtgcatat
    17641 aaaattgaaa cctactattt atgacattgc gaaaaaagct aatgtctcaa aatcaactgt
    17701 ctccagagta ttaaataacc agccaaatat ctctgataaa gcaaaagcaa gtgtacttca
    17761 agcaatcaaa gaattgaatt atgaaccgag caaaattgca agaggactgt caggaaatgg
    17821 ttttgacgcg attctagcat tatctcatcg tacttctcat acgacctatg gcaatccttt
    17881 tttttcagat gttattcaat ccatttctgc cgtttctgaa caaaataact tcgacttgat
    17941 tctacaaacg gcaaaaacac cagaagaaga gctagaaaaa tgcgcgaaaa agatccaaga
    18001 aaaaatgatc cgaggaatta ttattttaag ctctacgatg gatgaaacac ttctttttga
    18061 attggatcaa tacaatattc ctattgtggt aatcggaaaa gtcggaaacg actacacgaa
    18121 catctactca gtggacacgg ataatttcca ggatagttac gatttggtgg aaacactgat
    18181 caagaatgga caccgtcata tcggctgtct ccatgctccg acagaagttt atgtatccaa
    18241 tgatcgtctc aatggttacc gtcaatgctt atttgatcac cacctagata tccgtaatga
    18301 atggattata gacggcggct ttcgcttaga agatagcatg gcagcaatga atgatttaat
    18361 gaagctacga gaaagaccaa ctgcgatttt ttctaccgat gcattaaaat cattgagtat
    18421 ttacaaagtg gcagaacaat ctggaaagca gatacctgat gatctttcga tcgcaggatt
    18481 cagtgatccc ttattgtcct ccctattttc gccacagttg actcggatag acattccgac
    18541 aaatcagtta ggaatcaaag ccacagaact tttattccaa ctaatccacc accaataccc
    18601 tgaagaaaaa agaatcattc tttcaaccga gattactctc acagattcga tccaacaaca
    18661 agaaatgtag aacagaatta taaaagaaca gaaacactga ggataagtca gttactctgt
    18721 tcttttttca atcactcatc cctcgttacc ccctcccggt attttagcga atttttctta
    18781 ttttccatct gttttagata gctgtggata actataatgt ttgtgttaaa ctattttttc
    18841 cacagggaag aaaaaactat ttttaaccac gttgtgtata agtttttata gttcgttttt
    18901 ttaaacacat ttacccttat ttcaatccat gttatttggg gatacttatc tctaaaacat
    18961 aaatagaata acagacatgt ctactggttt tgttttattc gaggaattat tcaataatag
    19021 ctagcaaaat attaaaaata accgctctat cttcccatca atttattttg cattcaaaaa
    19081 atgatctatc tttacttttg ttgattgact tctcttggaa gatgatcgtt ccgatacctt
    19141 aattggatac gaaccgatgt tgttaagatc ctttcccact tttcttctag ttagacaaat
    19201 tattttagac aaaccaaaaa agaggttgtg acaataattt gtcacaacct cttttttaaa
    19261 atatctattt tgtttgattg ttcattgcgt cttctgcagc catttgcgct tcaatatctg
    19321 aaatactgta gacattttcg cttgctacac cgacttcttt tggttccatg tcacgataac
    19381 gttgcatacc agtaccagct gggatgatct tacctataat aacattttct ttcaaaccaa
    19441 gaagaggatc tttcttaccg cggatcgcag catccgtcaa gacacgagtt gtttcttgga
    19501 atgatgcagc agataagaaa ctatttgttt ctaatgatgc ttttgtgata cctagtaaga
    19561 caggtcgaga agttgcagga acgcctcctg agactagcgt attgtagttt tgatctttaa
    19621 agtctgcgat gtctaataat gtacctggta agatatctgt atcacctgga tccattacac
    19681 gaactttacg cagcatttga cgaaccatta cttcgatatg cttgtcgccg atttctaccc
    19741 cttgcatacg gtaaacacgt tgtacttcac gaagcaagta gttttcaaca gataatacat
    19801 cgcgaacttg caacaattgt tttggatcga ttgatccttc tgtcaatggt gcaccacggt
    19861 ggatcatatc gccttctgct actttcatac gtgctgtata aggaacagta tacgtacgtg
    19921 tatctgtttt tcctttgatt gttacttctt tggtacgtgt tgcaggatct tctgagatat
    19981 cgatgacatc ccctgttact tctgtgatca ctgcttgccc tttcggatta cgtgcttcaa
    20041 agatttcttg gacacgagga agaccttgag tgatatcgtc tccggcaacc ccacctgtat
    20101 ggaatgtacg catcgtcaac tgagtaccag gttcaccgat tgattgagcg gcgatcgtac
    20161 caactgcttc tccgacttca acgtcagagc cagttgcaag gttgcgtccg taacaatgtt
    20221 tgcagacacc atgttttgta ttacatgtga agacagaacg gattgtcact gtttcgacac
    20281 ctgcatcaac gatttgtttt gcgatgtctt cggaaatcaa ttggtttcct gcaatgatca
    20341 attcatttgt ttcaggatga cgtacttctt tacgggtgta acgtcccact aaacgatctt
    20401 ctaatgattc gatgatttca tttccctcgc ggatggcttg gatttcaaga cctcggtcag
    20461 taccgcaatc ttcttcacgg atgattacat cttgggccac gtcaaccaga cgacgagtca
    20521 agtaacctga atcggcagtc ttcaaggctg tatcggtcat ccctttacga gccccgtgag
    20581 ttgagataaa catttccaag acggatagtc cttcacggaa gttcgaaatg attggcaatt
    20641 ccatgattcg accattagga gcggccatca aaccacgcat tccggcaagc tgtgtaaagt
    20701 tggaaatgtt accacgagct ccagaatcag acatcatgaa gattggattt ttcgcttcca
    20761 aaccttccat cagtttttgt tggatgctgt cttttgcagc attccaaaca gcaatgacac
    20821 gttcataacg ttcgtcatct gtgatcagac cacgacggaa ttgttttgta atagtttcaa
    20881 cttgtttatg ggcattttca atgatttctt gtttttcatt cagtacactg atgtcagcga
    20941 tccctactgt gataccggca tgtgtcgaat gtttgtaccc taagtctttc atacggtcaa
    21001 gcatcttaga agtttctttt actttgaatc gtttgaagac ttctgcgatg atgtttccta
    21061 agtttttctt cttgaacggt aatactaatt cttgctcttt gatatgtgca ggaatatcag
    21121 taccagcttc aacaaagtat ttgtccggtg tttggacggt caagttgaag tctgttggtt
    21181 cattcagata agggaattca ggaggcatga tctcgttgaa gatgatctta ccaactgttg
    21241 tgatcatcgt acgttctttt tgccattcag taaatggttt ttcacctaat gagttagggt
    21301 tcacaccgat acgtgaatgt aaatgaacat atccgttgcg ccatgcgata acggcttcgt
    21361 tttgatctct gaagaccatt ccttcgcctt cgcgtccttc ttcttccatt gtcaagtagt
    21421 agttacctag aaccatatct tgagaaggag taacaactgg tttaccatct ttagggttca
    21481 agatgttttg agcagctaac atcaacatac gtgcttctgc ttgtgcttct tcattcaatg
    21541 gaacgtggac agccatttgg tctccatcga aatcggcatt ataagcttca catacaagtg
    21601 gatgcaaacg gattgcgcgt ccttgtacta aaactggttc gaatgcttgg ataccaagtc
    21661 ggtgaagcgt aggtgcccgg ttcagcaata ctgggtgttc cttgattacg tcttctaaaa
    21721 catcccatac ttcgtcttct tgacgttcga ttttacgttt cgcatttttg atgtttgaag
    21781 ctagttcgcg ttgaacaagc tcacgcatca cgaatggttt gaataattca atggccattt
    21841 cttttggaag tccacattgg tacatcttca agaaaggtcc aacaacgata acggaacgac
    21901 ctgagtagtc gacacgttta cctagcaagt tttgacggaa acgaccttgt ttccctttca
    21961 acatatgaga aagagatttc aatgggcggt tacctggtcc agtaactgga cgtccgcgac
    22021 gaccgttatc gatcaatgca tctactgctt cttgcagcat acgtttttca ttttgaacga
    22081 tgattccagg tgcattcaag tctaacaaac gtttcaaacg gttattacgg ttgattacac
    22141 gacggtaaag atcattcaag tcacttgttg caaaacgtcc accttctaat tgaaccattg
    22201 ggcgtaaatc tggtggaata acagggataa cgtccataac catccagctt ggttgatttc
    22261 ctgaagcgcg gaatgcttct aaaatatcca aacggcggat tgcacgtgta cgtttttgtc
    22321 cttgtgcaga tttcaattct tctttcaatt cggctgcttc tgcatctaga tcaacattat
    22381 caagtaattg tttaatcgct tctgcaccca tagcagcttg gaattcttgt ccatattgtt
    22441 cacgtttttc gcgatattcg cgttctgtca acaattgttt tttctctaaa ttcgtatttc
    22501 ctggttcgat gactacatat gaagcaaaat aaatgatttc ttccaaagcg cgtgggctca
    22561 tgtctaatac aagacccata cgtgaaggaa tccctttaaa gtaccagata tgtgaaacag
    22621 gagctgctaa ttcaatatgt cccatgcgtt cacggcgaac tttcgaacga gtaacttcca
    22681 ctccgcatcg atcacaaacg attcctttat aacggatacg tttgtatttt ccgcaggcac
    22741 attcccagtc ttttgtagga ccaaaaatcc gctcacagaa caacccttcg cgttcaggtt
    22801 ttaaggtacg atagttaatc gtctcaggtt tttttacttc accgtaagac cagcttctga
    22861 ttttttcggg agaagccaaa cctatttgca tactttcgaa tttatttaca tcgatcaaaa
    22921 ggggttccct ccatttcatt taatggcaaa ccgcaagacg gctgatcttc gaccgccttg
    22981 cagctcatag accggattag tctaaattat cttctgcggt ttcaaagttt tggatgacat
    23041 cgtctttttc atcttccact tgttcagcag ctttcttttc taattctttg gcagtttgct
    23101 gttcggcaaa ttttgtcaaa gcatcaacag tgatcaaatc gtcgtcttca tcgtccatgt
    23161 cgcgaagttc gatctctgtt tcttcgatat ccaagacacg catgtccaga cctagtgatt
    23221 gcaattcttt caccaatacg cggaatgatt ccggtacacc tggttttgga attggttcac
    23281 ccttgacgat tgcttcgtat gttttcacac gtccgactac gtcatctgat ttgtatgtca
    23341 agatttcttg taaagtataa gccgcaccgt aagcttccag tgcccatact tccatttcac
    23401 caaaacgttg tccaccaaat tgagctttac ctcccaatgg ttgttgtgta accaatgagt
    23461 aaggtccgat tgaacgtgca tgcaatttgt catcgaccat gtgagcaagt ttgatcatgt
    23521 acatcacacc gacagacaca cgtccatcaa atggttcacc tgtacgtccg tcgtacagaa
    23581 cagttttcgc atcgctggcc ataccggctt cacgaactgt ttcccatacg tcttcatcac
    23641 ttgcaccatc aaatactggc gttgctacgt ggatacctaa ctgacgtgca gccataccca
    23701 agtgtaattc caatacttgc ccgatgttca tacgtgaagg cacccctaaa ggattcagca
    23761 tgatatcgat tggtgttcca tcaggtagga atggcatatc ttcttccggc ataatacggg
    23821 atacaacccc tttatttccg tgacgaccgg ccattttatc cccttcatgg attttacgtt
    23881 tttgaacgat ataaacacga actaacatgt ttacgcctgg tgacaattca tctccagctt
    23941 cacgagtaaa gattttcaca tcgtgaacga taccgccgcc gccatgtggt acacgtaatg
    24001 atgtatcgcg gacttcacga gctttttcgc caaagatcgc atgcaataga cgttcttctg
    24061 cagataattc tgttacccct tttggcgtga ctttccctac aagcaaatcg ccatcttgga
    24121 cttctgcacc aatacggatg atacccattt cgtctaaatc ttttaatgcg tcttccccga
    24181 cattagggat ttcgcgtgtg atttcttcag gtcctaattt tgtatcgcgg gcttctgatt
    24241 catattcttc aatatggata gaagtgtata cgtcatcttt taccaaacga cggctcatga
    24301 tgatcgcatc ttcatagttg tacccttccc aagtcatgaa accaactaaa acgttttggc
    24361 ctagagccat ttcgccttgt tccatagatg gtccgtctgc cagagtatcc cctttttcga
    24421 ctttctcacc caaatgaacg attggacgtt ggttgtagct tgttccggag tttgaacgac
    24481 ggaattttgt tacatcgtat ttatctaatg caccgttgtc gcggcgtacg cggatttcag
    24541 aagcatcaac gtattcaacg acaccatcat gtttacataa caaagcagct cctgagtcat
    24601 gcgctgattt gtattccata ccagtaccaa cccatggtgc ttgaggattg atcaacggca
    24661 ctgcttgacg ctgcatgttg gcacccatca aggcacggtt ggagtcatcg ttttccaaga
    24721 aaggaataca tgctgtcgca acggcaacta cttgtttagg tgatacgtcc atgtagtcta
    24781 ctttatcaat agatacttct aagttttcac tttgagcacg agccataaca acgtcttgag
    24841 caaatgttcc atcttcattc aatgggctgt ttgcttgggc tacgatgtaa tggtcttcga
    24901 tatcagctgt caagtagtcg atttggtcag taacacgacc tgtttcacgg tctacacgac
    24961 gataaggtgt ttcgataaat ccgaatttat ttactttcgc ataactggac aaactattga
    25021 tcaacccgat gtttgggcct tcaggagttt cgatcggaca catacggcca tagtgggagt
    25081 agtgaacgtc acgaacttca taaccggcgc ggtcacgagt caatccgcca ggtcctaagg
    25141 cgaaaagacg acgtttgtgt gtcaattcgc caagagggtt tgtttgatcc atgaactgtg
    25201 acaactgaga agaaccaaag aattctttga tacttgccac aactggacgg atattgatca
    25261 attgttgagg tgtcaatgtt tcagtatctt ggatcgacat tctttcacga accacacgtt
    25321 ccatacgtgc tagcccgata cggaattggt tttgtaaaag ttcaccgact gaacggatac
    25381 gacgatttcc taagtggtcg atatcatcga cattgccgat accttccatc aagttcaaga
    25441 aatagctcat tgaagcaatg atgtcggctg gacgaaccgt ttttacagct gcttctggat
    25501 agccgtttcc gatcacgttt acttcacgtt cagggtctct tggtgaaaat actttgatga
    25561 cttggacagt cattggatct gtgaccacac cgtcttctga tggatagtaa gtcactgaat
    25621 ttaggccatt atcaatgaaa ggagctagtg tttccatgac ttggtgagtc agaactgttc
    25681 ctttttcaac gatgatctcg cctgtttctg gatcaactaa tgtttcagct aatgtcagat
    25741 tcaatagacg tgttttcaaa tctaattttt tgtttacttt gtaacgacca acgtttgcta
    25801 agtcgtaacg ttttggatcg aagaaacgag cattcagcaa gttacgtgag ctgtctgctg
    25861 tttttggttc gcctggacgc aaacgttcgt aaacgtcttt caaaccttct tctgttcttg
    25921 agtcgcttgc gtttttatgc aagtcttttt cgactgtatt gcgtaatgtt tcgctgtcgc
    25981 cgaagatttc aaagatcgta tcatcggaac caaatcctaa cgcacgaact aatacggtta
    26041 aaggaatctt acgtgtacgg tcgatacgta cataagagat gtcttttgca tcagtttcca
    26101 tttctagcca agctccacgg ttagggataa cagtagaacc gaatccttct tttccgtttt
    26161 tgtcgacttt tccatggaaa tagacacctg gtgaacggac taactgagaa acgattactc
    26221 gctctgcccc gttgatgatg aatgttcctt gttctgtcat taatgggaaa tcaccgaaga
    26281 atacttcttg tgctttgatt tcgccagtct cgcggtttgt caaacgcaat gttacatgta
    26341 atggtgcaga gtagtttgca tcatgcgcac gagcttctgc tactgtatac tttggttctt
    26401 tcaattcata atctacaaat tccaaagata gattgccatt gaagtcatcg attggcaaaa
    26461 tgtcttcaaa catttctcta agtccctcat ctaagaacca ttggtaagag tctgtttgaa
    26521 tttcgatcaa atttggtaat tccaacactt cactgattct tgcgaaactt ctacgttcgc
    26581 gatgttttcc gtattttact acgtgtccag ccaagctctt cacccctcta aaatgtcatt
    26641 caaaaagact acccgatcaa ttgttacaat aatatttcaa atattatttt taggtaacaa
    26701 cagacatttt ttgcggcttt taggcaaaaa aaaaccaaaa atagaacatt ctcttgaaac
    26761 cttacttcgt gagcttctac ttttgtttcc ttatattaaa gccgaaacgg acaatattt
//
LOCUS       DAYYHX010000015        26305 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000015 DAYYHX010000000
VERSION     DAYYHX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.015"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..927)
                     /gene="era"
                     /locus_tag="ACHA6Y_000462"
     CDS             complement(28..927)
                     /gene="era"
                     /locus_tag="ACHA6Y_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM8986580.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(942..1337)
                     /locus_tag="ACHA6Y_000463"
     CDS             complement(942..1337)
                     /locus_tag="ACHA6Y_000463"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8986581.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(1321..1797)
                     /gene="ybeY"
                     /locus_tag="ACHA6Y_000464"
     CDS             complement(1321..1797)
                     /gene="ybeY"
                     /locus_tag="ACHA6Y_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM8986582.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(1814..4006)
                     /locus_tag="ACHA6Y_000465"
     CDS             complement(1814..4006)
                     /locus_tag="ACHA6Y_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM8986583.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(4030..5001)
                     /locus_tag="ACHA6Y_000466"
     CDS             complement(4030..5001)
                     /locus_tag="ACHA6Y_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM8986584.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            5707..7086
                     /gene="brnQ"
                     /locus_tag="ACHA6Y_000467"
     CDS             5707..7086
                     /gene="brnQ"
                     /locus_tag="ACHA6Y_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8986585.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(7242..7688)
                     /locus_tag="ACHA6Y_000468"
     CDS             complement(7242..7688)
                     /locus_tag="ACHA6Y_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM8986586.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(7715..7891)
                     /gene="rpsU"
                     /locus_tag="ACHA6Y_000469"
     CDS             complement(7715..7891)
                     /gene="rpsU"
                     /locus_tag="ACHA6Y_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM8986587.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(8053..8481)
                     /locus_tag="ACHA6Y_000470"
     CDS             complement(8053..8481)
                     /locus_tag="ACHA6Y_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8986588.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            8573..9433
                     /locus_tag="ACHA6Y_000471"
     CDS             8573..9433
                     /locus_tag="ACHA6Y_000471"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM8986589.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(9517..9867)
                     /locus_tag="ACHA6Y_000472"
     CDS             complement(9517..9867)
                     /locus_tag="ACHA6Y_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8986590.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(9883..11610)
                     /locus_tag="ACHA6Y_000473"
     CDS             complement(9883..11610)
                     /locus_tag="ACHA6Y_000473"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8986591.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(11823..12749)
                     /gene="trxB"
                     /locus_tag="ACHA6Y_000474"
     CDS             complement(11823..12749)
                     /gene="trxB"
                     /locus_tag="ACHA6Y_000474"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM8986592.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(12894..14327)
                     /locus_tag="ACHA6Y_000475"
     CDS             complement(12894..14327)
                     /locus_tag="ACHA6Y_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8986593.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(14327..15730)
                     /locus_tag="ACHA6Y_000476"
     CDS             complement(14327..15730)
                     /locus_tag="ACHA6Y_000476"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8986594.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            15960..16862
                     /locus_tag="ACHA6Y_000477"
     CDS             15960..16862
                     /locus_tag="ACHA6Y_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8986595.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(16903..18789)
                     /locus_tag="ACHA6Y_000478"
     CDS             complement(16903..18789)
                     /locus_tag="ACHA6Y_000478"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8986596.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(18886..19737)
                     /gene="licT"
                     /locus_tag="ACHA6Y_000479"
     CDS             complement(18886..19737)
                     /gene="licT"
                     /locus_tag="ACHA6Y_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8986597.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            complement(20195..21979)
                     /locus_tag="ACHA6Y_000480"
     CDS             complement(20195..21979)
                     /locus_tag="ACHA6Y_000480"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8986598.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(21979..23730)
                     /locus_tag="ACHA6Y_000481"
     CDS             complement(21979..23730)
                     /locus_tag="ACHA6Y_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8986599.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            complement(23872..24096)
                     /locus_tag="ACHA6Y_000482"
     CDS             complement(23872..24096)
                     /locus_tag="ACHA6Y_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM8986600.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            24348..25934
                     /locus_tag="ACHA6Y_000483"
     CDS             24348..25934
                     /locus_tag="ACHA6Y_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM8986601.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         7400 a         5665 c         4678 g         8562 t
ORIGIN      
        1 tacttaacct ataagaagtt cagaatatca gtattcgtct tttcggtagc caaagtcttg
       61 cagatagact tttttgtctc gccagtcttt ttggactttg acccaaagtt ctagaaatac
      121 tttgtctcct aaaagatttt cgatatcctt tcttgctttc gttcctactt gcttcagcat
      181 ttttccgcct ttgccgataa tgattccttt ttggctatct ctttcgacga tgatcgtggc
      241 ctggatatgg attttatcat tctcgtttcg cttcatcgaa tcaacaacga ctgcgacaga
      301 atgaggcact tcgtctcgtg ttaaaaacaa gactttctca cggatcaatt cagaaacgat
      361 aaagtattca ggatgatcag tcacttgatc atctggaaaa tattgtgggc cctctggcat
      421 ttcactgact aatacatcca ttaagcgttc gacattgttt ccttctgtag cagagatcgg
      481 aatcacttgt gcaaaaggca tctgcgtcga ataatcttcg atgattccca gcaactcgtc
      541 tggatggatc ttatcgattt tattgataat cagataaact ggagaatgca cgttttttag
      601 acgttcaata ataaaatcgt ctccccggcc gcgtttttga tcagcactga tcataaataa
      661 gactgcatcc acttctttca acgcactgta agctgcttct accataaaat ctcctagacg
      721 atgctttggt ttatggattc ctggtgtatc gataaaaatc agctgagctt ctggtgtagt
      781 atatactcct tggattttat ttctagttgt ctgtgccttg tcactcataa tggcaatttt
      841 ttgacccacg attcggttca ataatgtgga ttttcctaca ttcgggcggc cgacgattgc
      901 gacaaaccct gatttatgtt ctggcatata cgttctcctt tttaattaaa caacaaatga
      961 tatattttag ggacaaataa aatcaaccca atgatgacag caaaacaagc ggtcactaaa
     1021 actgctccag ctgccatatc tttgattttt ttcccaatcg gatgaaaatg aaaatccgta
     1081 aacatgtcca ctacattttc aaaaacagta ttgatgatct ctactatcca aacgagaaaa
     1141 acagctaaaa gcagccataa ccactctatc cgatcaagct ggaaaatcac gcccataaat
     1201 aaagcaagga taccgaacgc aacatgtttc ctcatattgc gttcttcctc aaaaacggtt
     1261 ttgactcctt ggacagcaaa ttccacagac atcaggaaat gtttgttttt ctctacgttt
     1321 ttatcttttg agtccatagg catccaagat ctctttctgt aaaccaaaca tttctttctc
     1381 atcttctggt gtcatatggt catacccgtt gatatgcaag aatccatgta ccgctaaaaa
     1441 gccaagttcc cgatcatatg aatggccgta agcttctgct tgttcagcag cacgttcaat
     1501 agaaatcatg atgtctccta gtacacgagg caaaggattc tctccatctt caaaaatcac
     1561 tgggatttcg tcttcgcctt cttcttccag agcaaaactg atgacgtctg tcggtgcatc
     1621 ttttccacgg taatcacgat tgattacttg gattgccgca ttgtccatga aagtaacaga
     1681 catttctgtt tcctctggca gctttaaata agatgctgca aattgcagca agtcatcgat
     1741 ttctttcatt ttctcttctg agacttggtt tgtttcatcc ataaaggtaa tatccattat
     1801 acatcgcttc tttctattct tcttgttctt cctttatctt ctcaacttct gttttcattt
     1861 taggatattt gatccgagaa tggaaggtgc tacttaaacc gctgatcaat gatttttcca
     1921 cttttcggat ttctttcaag gtcagtccac tatcgtctaa ttgaccatcg gaaatacgct
     1981 cttcgataag attatggaca aaactttcga ttttttcgct cgtcggatga tccattgccc
     2041 gtactgcagc ctcacaactg tcagcaatat tgacgatccc tgcttcacga ctttgcggtt
     2101 ttggtccagg ataacggaat tgctcttctg ttacttccgg attgcgttct ttcgctttga
     2161 tgtagaaata acgcattagc gtggttccat gatgctgttt gcaaatatcg atcaccattt
     2221 gcggcatatt ataatcttcc aatattttgg ctccatctgt cacatgtcca aagataatat
     2281 gtttactgtc ttctggcaat aagaaattgt gcgggttttc cgctcctgaa ggcaagttct
     2341 caacgaaaaa gctagcatgt ttgatcttgc cgatatcatg ataatagcaa gctactcgag
     2401 ttagcaaaga acgtccgccg atttctgcta cagcgttagc acttagatta gctaccatca
     2461 tactatgatg ataagtacct ggcgcttctt ccagcaactg tttcaataaa ggatgatttg
     2521 gattgctgag ttcatttaat acaattacac tgtcatcatt gaccatcaat tcaatatatg
     2581 ggtgtagacc gatcgtcagc aagaatgaga agagtgcacc agtgaacccg cagacaagca
     2641 ttaaccaagt cgttgcatca ccaaagctca ttccttgata gacgatcaaa atgacatcca
     2701 tcatcacagg aaaaataatg acccacatgg cagcagataa tccttgttct gagatccgtt
     2761 tgcgtctgac aatcgttgcc atgagcccag agaaaacata agacatcaag ataatcgtta
     2821 gaaagttcgt gccaatcgaa tcataaaaaa tgaaaagtgc agatactgct tggaaaatac
     2881 cagccatgat ccctgctctt cggttcaaga aaaagcttaa aacaagtggt gcaaaagcag
     2941 cgggataaaa cagcgggata taagtagctt gttccgtttg gaataattgg aaaaatttca
     3001 tcagcactat gctgactgac atagctgtca catagaaaag cacgtatttt gttctttctc
     3061 ctgcattttg gaattgaagt gaattataaa tcagtacgcc gatttgaagc aaaacagcta
     3121 aaaccatggc aatgagtggg aaaatcgaag tcgtttgatt cgtcaatcct agtaattcca
     3181 attttttcat cgctgaagca tcaatttgat ttccttcgcg aacaatgatt tctccttgga
     3241 agatcatcac tggttgtaca gcttctcgtg cagattgttt taattcttct gtctttttct
     3301 cattcaatac atcattgact acaatgcctt gatccactaa gtagcgaatc atttgttgct
     3361 gctctgttgt gacattcaaa tactgaatct gatcttctgc ttcttgttta aaagcttcca
     3421 attcgctttc acgaacttgt ttactcatct gttcgtcaat gagcttcaaa ctttcatccc
     3481 taaccgtgtg aatttccgtt tcagtcattt caaatatcgt cgaataaaaa ttgttcggca
     3541 gtttttggta aaacgcaacg ttttctgcat tcacgttttc aaaatttttc ttcagtgtag
     3601 caatccgctc ttctgtagta ggtttcggga tattttcatc ttccttcgcc ttatctacct
     3661 tgtcattata agatttgttg atggtatcat tcgtcttatc gatcaattcg aataattgct
     3721 tgatccgatt gttctggtca tctgccagat ctttttgata agtatattct ggagtcactg
     3781 cttctgctgc cagttttctt ctttgttcgg tttcttccgt gttctcgatc gttttattcg
     3841 cacggatact ctcttctgct acttggcctt ctctgaaatc attggccttt tgtttgacac
     3901 tactaaatgt aacgccgcag acaatgataa aaaacaagaa cagaaggaaa ggcaatgctg
     3961 cctttccgag tttttcttgt agtttctgca gtgttttatt caacatgtgc ttatcctcct
     4021 aagcaaagct tattcttgat gaagatccgc cttttcataa gctttgatga tttcggcaac
     4081 tacagggtgc cgaacaacgt cacttgcttc aaaattaaca aaatcgattt ttttgatttg
     4141 ctttagtgtt cgttctgcat gaacaagacc acttgtcgtc cctcgaggca agtctatctg
     4201 acttgtatcc ccattgacaa tcatttttga ttgataaccg agacgggtca agaacatttt
     4261 catctgagcg actgtcgtgt tttgtgcttc gtccaagata acgaatgcat catccaatgt
     4321 ccgcccccgc atgtaagcta atggtgcgat ttcgattacg cctcgttcca tcaatcggtt
     4381 cgtatgatcc aatccaaaaa tttggtataa agcatcataa acaggccgca gataaggatc
     4441 cactttttct ttcagatccc cgggtagaaa gcctaggttt tcccctgcct ctacagcggg
     4501 ccgcgtcaaa atgatttttt gcacttcgcc tttttttagg gcagcaattg ctaatactac
     4561 ggcaagaaac gttttccctg ttccggctgg ccctacacca aatacgatgt catgtttggc
     4621 taccgattca acgtatttct tttgaccgct gttttttacc cgtataggtt ttccattacg
     4681 gtctttgacg atctcttcct catacatctc aacaaaataa tctaatgtac ctttttgccc
     4741 cattttcaat gccgtcacga catctggtgt acttacgtgg attcctcgtt tgatcaattc
     4801 ctgtaatgtc cgaatgactg aggcagccaa ggaaatctgg gattgttcac caatgatctg
     4861 aatgatttct ccacgtgtat ggatggttgt ttccgttgtt tcctcgatca gttttatatg
     4921 cttgtcgtgt gcacctaaaa gcatgctgac atcatcagca gctgtcaatc taatctctaa
     4981 agaactagac tcttccgtca aaaatagaca tccccttttt ttcgattcat tctttcctat
     5041 tttaccatac tcagaagaaa ctatatagca aataagaaaa ggttgtaaaa gagtcgttta
     5101 accatacgta ccaagaaacg ttaaaaaata gctgtttcca ttcgctaaag gtctcgtttt
     5161 gataaaaaga atctgagaat gtaagttata tgaaagtgga gaacaaaata ttccgtttca
     5221 ttcagaggta ttctctaggt atttttcttg tttcaaaaaa agaaaacaaa atagttaaaa
     5281 tattcttgca tttcaaaaaa aacaggtgta ttattacgtc aacaaataaa catacagcaa
     5341 agcgtcgagg aaaacagtag gctattcaac gatttattcc cagtgagtca tgtaagtgag
     5401 aaatgacaat aaatcgaata gacgaacatc atttccgagt caatgtcgaa aacgtagatc
     5461 attgtggctg cctccattat caggctagcg gataacttta tcaaaaagcg tccgtgaatg
     5521 aagaagcttt tttaagcgaa aaaaggtggt accgttctag aatctagagc ccttttccca
     5581 agtggaaaag ggcttttttt tatattttaa ggtggtggtt ttcatggaat ctgatattag
     5641 tggcattact aattgctgca acgtttatat cgtcaatcaa aatgataatt attggaggaa
     5701 aaatttatgg aagaaacaaa aacttattca tggaaacaac tattgatggt cagttcactt
     5761 atcttcggga tgtttttcgg gtcaggtaat ctaatctttc ctgtccactt aggtcaaatg
     5821 gctggcgcaa actgggtcag tgctggcgta ggatttgcca tctcaggcgc tcttttccca
     5881 ttactagcta tcttagctgt agtagtgaca aaaagtgatg gtctgtatga cttagctaaa
     5941 cctgtcggaa aatggtatgc tgccttgttc ttaatccttg ttcatttgac gatcggacca
     6001 tttttcggta caccacggac agctgctact gcttttgaaa tggctgctaa accatttttg
     6061 cctgaaaaat ataattcatt aggtatgttt ttattctctg ctcttttctt tttacttgct
     6121 tatttcctta ctgtccatcc cacacggttg gtcaaatacg tcggaaagta tctaaactcc
     6181 ttgtttcttg cattattagc aatcattttt gtcgtttctt tattccaccc aatgggaaat
     6241 ctaaatcaat ctgctgcaga tacttaccaa acaaacgctt taacaaatgg tgtcctagaa
     6301 ggttataaca cggttgatgc agtagccttg cttgccttaa gtgtcacttt tgtccatgca
     6361 gtccgtgacc ttggtttcaa agataaaaaa gtcacagaat taacagcaaa agctggtagc
     6421 ctgagcgttg cgctagaagt catgatctat tttggtcttg ttcttctcgg tgcgatgagt
     6481 ttgaataaga tgtctttatc agaaaatggc ggaactgcac tttctcagat cactaatttt
     6541 tatttaggta acttcggcgc tagcttctta ggagtgatgg tcacattagg tgtcttcact
     6601 accgctatgg gactcgttgt ttcttttgca caagatttcc ataaactgtt ccctaaagtc
     6661 agttatatga catggctgcg gttaacgact ttcgtctctt ttgtcgttgc aaatgctggt
     6721 ttggacaata tcatccaatg gtctttacca gtattgatgc ttttgtatcc attgtcgctt
     6781 gcgttgattt tgctttcctt aacagcaaaa tttttccaaa agacaccatt tgtctatcaa
     6841 gtaacgatgt tgtttgctgc tgttcccgcc gtattggata tgctggcaaa ttcgcctgct
     6901 ttagtgagtc aacaaagagt tgtagcaagc atgctcgaat tttatcatca tcacgttcct
     6961 tttgctgcat tgggacttgg ctggatggtc ccaacgttac ttgggtatgc tggcagctta
     7021 ctcttctatt acgcctatcg cctatcaggg tataaacaag aagcaaatga actaccagaa
     7081 gaataaccag atcaaacaca atttaacata agaaatatta aacaggaaaa aaacagctag
     7141 atgaaagaat cctattcttt cgtctagcta ttttttagat ctctttttta cgagcacaaa
     7201 aaaactgcgg acaatgattg tccgcagttt aaatgataaa attaagattg taatagttct
     7261 ttcacaatgg catttacttg gtttccatct gcttttcctt tgactttagg catgatcgca
     7321 cccatgactt tgccaaattc tttcattgat gaagcaccag tttgcgtgat tgcttcttga
     7381 acaagttgtc gaatttcttc gtcggaaagt tgttccggca tgtatttttc gactatcaca
     7441 atttcacttt ttactttttc agctaaatcg tcacgtcctg cttcttcaaa ctcatgcaaa
     7501 gaatcacgtc gctgtttcat ttcgcgagac aacaccgtta attcttcttc ttctgtcaag
     7561 tcgtgacctt ctttaatctg ctcgttttgt atagatgact tgatcattcg gatgacttgt
     7621 aaagattcct tatctttcgc tttcatcgct gttttcatgt catcgtttaa tctacttagt
     7681 agtgacacac taccaactcc aattcatcac agaactagaa ttttttgcgt tttctagctg
     7741 cttcagattt tttcttgcgt cttacacttg gtttttcgta gaattcacgt ttacgagatt
     7801 cttgtaaagt acccgctttt gacacggaac gtttgaagcg acgaagagca tcgtcaagtg
     7861 attcgttttt gcgaacgact gtttttgaca tgttaattcc ctccctccga gcttaaaaag
     7921 taaccgtaat tgttattgaa atatatctca aaaaacaaga ctgtcctgta aatattatat
     7981 ctaatgctgt tttgaccgtc aaggaaatct tgcttttttt taaaattatc aaatgttatt
     8041 ttatttaaat ccttattgac aattttcaca tcttccgaaa atctcgaatc tatgcccttc
     8101 gatcgtacat ccaccgagct gttcttcgaa ataattcatt ggacacatat ggatttcttt
     8161 cgttgttccg cacaccgtac agataaagtg atggtgatgg ccgatttccc cttgacagca
     8221 atgaaaacga aacttcatct ctccatttaa atcggtttct tccagtaaat cgatttctgc
     8281 aaaatcatgg agatttcgat aaatcgtatc gtaacttact cctttgaatt cctgattcat
     8341 atagtcaaaa acttctttag cggatacgta gcgattgcgc ttcactaaat aagttaacaa
     8401 tgcttctctt tttttagtat atttaaaacc gttatttttc aatgtttcta gtgattgttc
     8461 gatcaacgtc tgttttttca tgacttattc ctccaaatca aaatgattac gaagtacaca
     8521 ttctagagta tggtataata aacataaaaa aaatgcaagt gaggttttat ccatgtcttc
     8581 cgattctaca gaaatcgtta ctttttttgt catctccgac tctgctggag aaactgcgac
     8641 aaaactagcg caagccacta tggcccaata tcctagcgtt gagttcaatt tattccgcag
     8701 aacatttgtg acagatgaga aaacattgat gcaggcactg caagatgcac tagaagaaaa
     8761 agctcttgtc ctgcacacat tgatcaatca agaattgatc gagatgacgc gttctttttg
     8821 tgaagaacat caattattta gttttgatgt gatgactccg ccgatatcag aaatcgagcg
     8881 cttaactggt atcaagccca ttcggcaacc tggcgccctt catttattga atgagaacta
     8941 tttcaaacgg atcaaagcaa tggaatttgc tgtaaaatac gatgacggga aagatcctcg
     9001 tgggtttcta gaagcagatg tcgttttgct aggcgtatcc cgaacttcca aaacgccttt
     9061 gagcttgttt ttagctaaca aaaatttaaa agttgccaat ctacctttga tcccacaagc
     9121 acatattccc aaacagcttt gggagatcga tcctaaaaaa atcgtgggac tgacgaataa
     9181 cccagatatt ttaaacaata tccgtaaaga acggatgcgc tcttacggat taaatcctga
     9241 tactgcttat tccgatatcg aaaaaatacg agctgaactc gattttgcga atgatcttta
     9301 tgaaaaattg ggctgcgtag taatcaatgt cgcttcttta tctattgaag aaacagcttc
     9361 gcttattttg aatgctttag acctagaaga ccatagttat tatggaactg aaaccaccga
     9421 agaaacagac taacaatttt tctttaaaaa aactggactg tgtcagcgaa gaagattcgt
     9481 tgacacagtc cagtttgtct ctatttgcac ctatcgttat tgatgttcca catgtttgag
     9541 tgtctggttc aaaatatcca gtacgtgaga atcatcaaga ctatacaaaa tcgcttttcc
     9601 ctcacgatgg gacttgacga catggttttc tcttaataaa cgcagttgat gcgatacagc
     9661 agattgttcc atttccactg cttggatgat tgctgtcaca ttttgttccc cgtctttcag
     9721 atacagcaga attttgagac gagtggggtc actcaacatt ttgaatagtt ggctgacttt
     9781 ctctactttt gttttttgcg tgtctggcaa tccattcacc tccaacttga aaaaagactg
     9841 acttctaaag tttaccactt tttccatcag tctcaagttt ttttattctt ctgtcatctt
     9901 ttggataatc gtttcataag cggctacttt ttcagctgcg tcttctgaag agtttccttt
     9961 tacaccgata taaaatttga ttttcggctc agttcctgaa ggacggatag cgatccagcc
    10021 attttcttcc agcgtatact tcaatacgtc agatggcggt gtcgttagtt tttctacgtt
    10081 tccttcacgg tctgttctcg tcaactcttt gaagtcttct gtttggatga cttttgctcc
    10141 ggcaaattct gttggtgctt cttcacggaa tttttccatc aaagcagaga ttttcgcaga
    10201 accttcttgt ccgctcattg tcacagaaat cgttttttct gcgtagtagc cataggtttc
    10261 aaaaatcgtt tgcaagccat catacaacgt tttgccttgt tttttatagt aagctgctac
    10321 ttctgctaat aagagcaatg cttggatcgc gtctttgtcg cgaacgaaag gtttaactaa
    10381 ataaccgtaa ctttcttcga atccgaacat aaaagtatgg gaatgatctt cttcaaattg
    10441 ttgaattttt tcagcaatga atttgaatcc tgtcaataca ttgaacatgg tagccccata
    10501 gtgttccgca attgctgtag gtaattcgct agaaacgatc gatttcaata ccgctgcgtt
    10561 ttcaggtaaa gtgcctcgcg attggtgtgc ttccaaaata tattgagcaa acagtgctcc
    10621 gatttggttt ccggtcaata cttcatagga gccatttggc aaacgaacgg ctgcacctaa
    10681 acggtctgca tcaggatctg ttgcaatcag caaatcagcg ccttctttct cgccaagtcg
    10741 tatcgcatac tcaaaagcag agtgttcttc tggatttggt gatttgactg tagtgaaatc
    10801 tgggtctgcg acagcttgtt ctggaactag tacgaattgt tcgaatccag cttgtttcaa
    10861 cgcacgttcg cccaacattt ttcctgttcc gtgaagtggt gtgtacacaa gtttcaaatc
    10921 tttccccatt gtttccacca attcatgatc aatcgttact gctttgattt cttctagata
    10981 agcggcatcc acttcttctc cgatgatcgt gatcaatccg ctatgttcag cttcttcgtc
    11041 agataatact tcaactttta acggattgct gacttcacgg acatatttcg tcaatgcatc
    11101 tgcgtctgca ggaggcattt gtcccccatc ttctccgtag actttatatc cgttgtaagc
    11161 tgctggattg tgggatgcgg ttaccatgat tcccgcaaac gtcttcaaat gacggactgc
    11221 aaaagaaagc tctggtgttg gacgtagact ttcgaataca taagatggga tattgtgatg
    11281 tgctaatgtt ttggctgctt ccatggcaaa ttctggtgat tgatggcgtg agtcataagc
    11341 gatagcgaca cctcggcgtt ttgtttcttc gccttgtgta tccatgaaac gtgcaagtcc
    11401 ttctgttgct tgtcttaccg tatagatgtt cattcgattg atcccaggcc ctaagattcc
    11461 ccgcatgcct gcagtaccga attccaacgg tgcataaaaa gcatcttcaa gtttttcagg
    11521 atcttcaccc agatcattta attgtttttt taagttttct tctaaatttt cttcatgtac
    11581 ccattgttca tacacttgtt cccaagacat gaaaataaca cctctttctg aaatatggtt
    11641 cttaataagt atagcattca cttattagat atgaaagtat gaggaccaaa aaacgcttaa
    11701 atcaagaaag gattctttta tcaagcacgg ttgcaagtat tcgcataaat cggattcagt
    11761 agatttcacg cgaaaaaaaa gagtaaaaga acaattaaac cgttcttcca ctctttttaa
    11821 acttatgcag ttgtttcttc taaagattcc aagtatttat acacttgttg tccagcgatc
    11881 ccaccatcac caacagcagt agtgatttgt cgcaaatctt tttcacgaac atctccgatg
    11941 gcaaaaacgc ctggtatatt cgtcttcatt tcgttgtctg ttggaatcca gccagcttcg
    12001 ttcgtcaacc ctgcttctct aaatggtgca gttaatggat ccagtccaac atagatgaat
    12061 actccgtctg ctgccaaatc aaactcattg ccggttttga cattacgtgc tttcacacct
    12121 gtcactacca tttcattccc aacgatttcg tcaacgactg tatcccacat aaaggaaatt
    12181 ttttcattcg caaatgcacg gtcttggatg atcttttgtg cacgtaattc atctctacgg
    12241 tggacgatga ctacttcaga agcaaattgt gtcaaataga tggcttcttc tactgctgag
    12301 tctcctcctc cgatcactac tagacgtttg ttacggaaaa acgcaccatc acaaactgca
    12361 cagtaagaaa cgccacgtcc agcaaattct tcctcgccag gtacgcctaa tttacggtgg
    12421 atacagcctg ttgcgataat gactgttttt ccttcgtaag ttttatcttc acaaataact
    12481 tctttgtggc tgccgtgatc cttgatatct tgcacgattc cataagcatt ttctgtccca
    12541 aatcttgaaa caccttcata catcttataa gctagatcag gacccattat tgaatcgaaa
    12601 ccaggatagt tttcgatttc tgccgtattg ttcatttggc cgccaggggc gccttgttcg
    12661 atcatcaata cggataagtt tgaacgtgaa gcatataaag cagcagtcat cccagcagga
    12721 cctgcgccaa taacgattac atcatacata tgtagattct cctttactta atcaagtaag
    12781 catactttac agccaatgcg aaaaaaagtc tattgttctg cttgcgtttc tcgaatagct
    12841 ttattatact cgaaaaatcc gctgaaaaac aatagacttt tataaatcat tgatcaatca
    12901 ttcgaaagat tttctccatt tgaatcaatt acttttttat accagttgaa tgatttcttc
    12961 ttcgaacggg ctaatgtgcc atttccttcg ttgtcacgat cgacataaat gaatccgtac
    13021 cttttcttca tctcacctgt accagctgat actaaatcaa tacatcccca agtcgtatag
    13081 cctaatagat cgactccatc taattctact gcatctttca ttgcctggat atgtgctgcc
    13141 agatagtcga ttcgatagtc atcttccaca taaccattct catcgggcgt atcgactgca
    13201 cctaagccgt tttctacaat aaatagtggt ttttgataac gatcataaat atcattcatc
    13261 gttattctca agcctaatgg atcaatttgc caaccccatt cacttgcttc cagatatgga
    13321 tttttgacag aagcaaagat atttcctgca gtctgttcca agagttcgtt gtcagtagtc
    13381 gaaactcgag aagaataata agaaaaggag ataaaatcaa cagtatgttc tttcagcaat
    13441 gcggcatccc caggttccat tttgatctga atgccatttc gttccatttc tttcaatgca
    13501 taagcaggat attctcctcg tgactggaca tcaatgaaga aataattttc tcgatctgat
    13561 tttctagaag cccaaacatc ttcaggattg caagtgtatg gataatatgc tcctgctgcc
    13621 agcatacagc ctactttgtt ctccggatct acttcatgag caatcttcgt tgcgatggca
    13681 cttgcaacca actcatgatg tgccgcttga tatttgattt gttcttcatt ttctccttct
    13741 tcaaaataca gccctgcgcc catgaatggt gcatgaagga tcatattgat ctcattgaat
    13801 gtcaaccaat atttgacaag tcctttatag cgattgaaga tgactcggca cagattttca
    13861 taaaaaccaa cgagttctct ggaacgccac gcaccatatt ctttgatcaa atgcatcgga
    13921 caatcaaaat gtgtaatcgt cacaagcggc tcgatcccgt acttttgaca ttctttaaag
    13981 acatcctcat aaaatttcaa accagattca tttggctctt tttcatcgcc cattggaaaa
    14041 atacgactcc aagcaatgga catccgataa gtcttgaacc ccatttctgc aaataaagca
    14101 atatcttcct tataatgatg atacatatcg atcgcaattt ttgctggata aaaatgattt
    14161 tcatcaaatt caaacatttt cttttttcca gtaatgacag gaaaacggtc ttctccgatt
    14221 ggcacaacat ctacgttcgc taacccacgt ccgccttcat cataaccgcc ctcgcattga
    14281 ttggctgctg tagctccgcc ccataaaaaa tcttttctga atcccatcat acttcctcca
    14341 ttacatgtgt attgcttgat aattctcttg ccagttcatc ttggacttcg attccttctg
    14401 cttttaaccg gctgattgtt tccttgaatt gcggtaaata ctgctgatgt gcaataaaca
    14461 actcattcat gatccgtttt gctgtctttc ctgatcgtat aagcggattg atcgtaaacg
    14521 cttgtaaagc tgttccataa tcaccagtca cagctgcttc gatcgtcagc aattccatat
    14581 tcttcattac ttgcaaccag cctctttcag cagcaggaag ggaaccaaac gctacatttc
    14641 gtgcgccttg tgcgccaaca taggcagtga cttctactac acaatctgcc ggcagatcag
    14701 gaacagctcc atcattcttt gtagatacca cgatctgtgt ttctttattt ccatagatcg
    14761 aagcaatcgt ttcacaggca gcatctgaat aataagcacc acctcgtttt tgcaattgtt
    14821 caggtttatg atccaattct ggatctttgt ataattcaaa cagctctgct tctgtttctt
    14881 tcacttgctg cgctcttgta ccaatcgttt gatattcttc taaagcatgt tccagcattt
    14941 cttcttcacg gtagtaatag cggtggtagc cacaagggat catgttcatt tgttttaatt
    15001 gttctttgaa aaacgggata tcaaagatat ttttcggcaa tccattgtct tcatcgtata
    15061 aacgatcaat caattctgcc gtgcggtcat ttcctttatt atctgctact ttgtgccaat
    15121 ggaagtgatt tagaccggca aacttgtaga tcagctcttc ttttttcttg ccaagcattg
    15181 ttggttctgt catcatcgcc attactggca cattgcataa tcctatcact ttatcccatt
    15241 tgccatatct gataactgct tctgtcacca tcccgcttgg gttcgcgaaa ttgatcagcc
    15301 aagcatttgg acataaacgc ttcatgtctt caacgattgc caaaaccact ggaacggtac
    15361 gaaaagcttt gaatatcccg ccagcaccat ttgtttcttg cccaatcatc ccataataac
    15421 tagggatccg ctcgtctttg attcgtgcat ctaattgacc gacacgaaac tgagtcgtaa
    15481 caaaatccgc atctttcaat gcttcttccc gatccagagt tgcatagatt ttgacttcat
    15541 acggagaagc atcccacata cgctgagaca tctgactgac gatctcaagc ttttcttttc
    15601 cttcttctac atctacgagc cagatttctc gtatcggtaa ttcctcatat cttttgataa
    15661 atccttccat caactcgggg gtgtaactgc tgcctccacc aatcgtagcg attttgattc
    15721 cttttgccat gttctcactc ctttactttt tacactttta ttatcttttt tgtaaacaaa
    15781 acaaacaata gaaattcgtt tctttccagt aacatttaca aaagttagtg ttttctactg
    15841 gatttttgta aatatcttgt caaaaaatgt ctttattaag ctatataaca taaatccgtt
    15901 tacaaatcat tttcaaaaaa gttataattt acgtaaaaaa acaagaagaa ggtatctaca
    15961 tgttattacg tgaaaaaatc aaagaaatct ccttttctcc ttcagaaaat gaagtgattc
    16021 agtttctctt aagcgattct ccagcacttg gcgaaatgac catccaagag attgctgagc
    16081 gatgttatgt ccacccgtcc acactcatcc gtgtagcaaa aaaaatgggt tttgatggct
    16141 gggtcagttt tcgtaatgct tttttagctg aaacagccta tcttgaaggg aattttcaag
    16201 acgtagatgc gaatcttcct ttttccacca cagacggtat tatgacgatc gcaaataaaa
    16261 tggcttcttt agaacagatg acgattcaag atacactttc tctcattcat catgaccaac
    16321 tgcaaaaagc caaacaacta atgcttcaag caaaaaaaat cgtgattttc gccagtaact
    16381 cgaatacttt gatttctcaa gatttcatgc tgaaaatgaa gcgcatcaaa atggatgtca
    16441 cgattgtcac cacttttggt gaatcgttct atgaagcgta caattgcacg agtgatatgt
    16501 gcgggattct gatttcttat acgggtgaaa acaatgtgat ccttcgtacc gcagaaatat
    16561 tgaaacaaaa tcatgttcca attttagctt tgaccagtat cggtgattca acgctatctt
    16621 caatgagtac agccgctttg cgacttacga cacgcgaacg actgtattcg aaaattggta
    16681 attttacgat caatacatcg atttgttatc ttctagacgt gttatatagt tgtgtttttg
    16741 cagaaaatta tcaacagaat ttagtccatc taatttctgt aggacaaaaa tttgataaac
    16801 gccctatcag ctcagccatc atgaaagagc ctttcaattt tgaaaataaa gaagtggact
    16861 aaaaagaata attaagccta gtccattctt tcacctaacc gttcaggtca gtgcggtgat
    16921 aagtgcatcg tctggcccga ccgtctcccc ttgattttcc aaaatatcca aataatccgt
    16981 gtaattggtg acgatcacag gtgtttctaa actgtatcct tcctcttcga ttttcttttt
    17041 gtcgaagcgg agcaacagat cgccttgttt cactctttct ccttgctgca ccaagctttc
    17101 aaaatatttc ccttccaact gaacagtatc caatccgaca tgaatcagca attccagacc
    17161 tgcttcagaa accattccga ttgcatgttt cgtcgggaac agggtcatga ctgttccgtc
    17221 aaaaggggca cgtacttctc ctttttcagg gtagatcaag attcctcgac ctagtgttcc
    17281 ttgtgcaaaa gctgcatcct ttgctgcatt cagtggagcg atttgtcctt gtatcggtgt
    17341 ttttacaatt tctttacctg atcttttctg tctatcatct gtttcacctg cttcctcctt
    17401 ccaaaagaaa aaagtcagcg caaaggatac caccatcgca atggcgattc caatcaatac
    17461 attaatcaca tccgttaaat caccatctgg tctaatatag ttgggaattg taaagatgcc
    17521 taatccacct ggattccaag aagttaaatt cattaatcca ctatacgcgc cccctaacgc
    17581 accaccgaca caggtaaaaa taaatatttt tttacgtggc aatgtaaacc cataaatagc
    17641 tggctctgta atccccacaa taccagaaac aatggctggt attgcgattg ctttgtcttt
    17701 tgggtttcta agcttaaagt agagtgcagt aagtgctgca gtttgtccaa aagacactac
    17761 tgcaacaggt gtcatgatat tttgccaacc attcacagaa aattgcatca atcccattgg
    17821 tacgattgcc caatggagac caaacatcac tagaatttgc cagaagaagc ctacgattgc
    17881 tccataaata actggcgaaa gtgctaaaag tgtttcaaat ccagccatta aaatatcagt
    17941 tgcgaatgtg aatgctggtc caacgagtaa aaaacctaag ggcattgtaa taagaacggt
    18001 gaagaatgga acgaagaaat tcgcgatcat ctcaggtact aactttttaa ttggtttctg
    18061 aatttggcta gctaacaaca caataaagat aatgggaatg acagaagaag cgtagttacg
    18121 cgcaatccag ggaatgccga atactgtttg ataaataggt gcttcaaaaa tagtgccaga
    18181 aaatatggta gtcagtggtg cttctgctgc accactcaaa gcttccagcg acaagctagg
    18241 attcattaat gctgccccaa gcaacatccc tacgaattca tttactttaa atttacgggc
    18301 agctgttaaa ccgataataa caggcataaa ttggaataca gaatcaccga tattatcaaa
    18361 cataacaaat gtgccagaac cgcggtcaaa tgcgcctaag aagacaagta atgctgtcag
    18421 tccacgaatc atcccaccag cagctagaat agcaaggaat ggctggaaac agcccgatat
    18481 tccatcaata agcatatcaa acggtctcat tttttctgtt gaaacagcct catcaaaagt
    18541 aaggtttcct gcttttataa cctcttcaaa cacgactggc acatgattcc cgatgacaac
    18601 ttggtactgt cctcccgctc gcattaccgt cacaacaccg tccatatttt tcaagatctc
    18661 atcatttgct ttttcttcgt tttttaattt gaaacgaagc cttgtgatac aatgtgtcaa
    18721 accattgata ttttccttcc cgccgacatt ttctacaatt ttgctcgcta attcttgata
    18781 ttttcccatt tctattccct ccaatttatt agtggaaggt ttagccaact gaatgtcact
    18841 acccttattt catgcgttct tgttaattta tcctcctttt tttatttatt tacaacgatt
    18901 ctcatgcact aaacgcgcaa tatggatcgt taaatacatt ttttcgtccg tggacatctg
    18961 gtagttgaat atctcggcta aaaagtctgc tattttctca acgcaaccaa acgtatcctt
    19021 atattttttt tgaataacct ggtacaattc ctcagaaact tctcccactt ctgatttttc
    19081 tccatctagg acgcgtgtca gaaaaaaacg taggtgtgtg acgaatcgaa aaaaatggac
    19141 tgaatcttca tcaaatgtaa tcttatagta atagcggacg atatttagaa tatcctgtat
    19201 tgttgtagta aacaggtagg tattgtcttg ttggctttga atgccttgtg cattgactaa
    19261 atgcaatgcg ataaatccgg cttcatcttc tgataacttg atgtggtatt tatccgagat
    19321 tttcttcact gtttttttcc cgatggcata ctctttagga aatagttgtt tgatatcata
    19381 cagcaataca tttggtacaa atattcccat tttgtgacgt tcgatagccg tatacaaatg
    19441 atccgtcaat gcaatataca ccgaatcatt caactggact tgtagagcct cttttgtata
    19501 ttcaatgatt tcacaagaaa tttctaagta ctcaagagga atcgcttgtg ccaattcttg
    19561 gaactgttgt gaaagtccgt catttgtcaa ctgaaatttt ttgtcgatgc atgagtcaaa
    19621 aatcgggtca cccactttct ttttgaatgc caaacctttt cccatcacga tcatttcctg
    19681 atttctttca tccctggtta ttacgacgtt gttattcagt actttctcaa tcagcatgtt
    19741 ttactccttt ccatgatata tagggctgta tccaaaacaa aaagaaaaac ccaaaaagcc
    19801 gtccatggaa ccatgggaga ctcatcaggt ttagcttgca tcaggcaatc actatccaag
    19861 ttgtatcgtt ttctttatat tatcgaaagc aagcactttt gtcaacgctt tcttagcggc
    19921 tattgggcac ttctgactag ctgtcgtgat tttttttaaa tctggtaatt acgacactaa
    19981 cgctcacaga tgaagaatct gggaagctct gttttatttt cactctagcg cttaggtcaa
    20041 aatgctacta tgctactgtc ccaacttcta atatacggtg ttccagtacc agcttatcgg
    20101 tgataagcca aatttatgca aaaatctgga aagccttttt tacaattttt ttcttatcac
    20161 ctaaagctga acggagccct gtcacaacca ctttttacac accactgctt tgcagtttgt
    20221 acatatctgc atacaaacca ttttgcgcta gaagtgtttc atgattccca cgttctacta
    20281 tatgaccttt gtctaaaact aaaatcaatt ctgcatcccg aatcgtggac agccgatggg
    20341 caatcgcaat cgttgtccgt ccttgtcgca tttttgtcaa tccttcttgg atcaatcctt
    20401 ctgtttcagt atcgatattt gctgtcgctt catccaatac tagtatttta ggatctgtca
    20461 cgattgttcg cgcaaaagtg atcagctgtc gttgtccgct agaatagctg gcaccgtgtt
    20521 cgatcacttt tgagtgatat ccttttggta attcatcgat gaacttgttt gcttgaacaa
    20581 actcagctgc atggcgaatc tgttcgtctg tgatagttgg attcatcatc cggatatttc
    20641 ctgcgatatc tccataaaac aagaaggcat cttgcaagac caatcccatt ttcttacgta
    20701 actcttccat tggatattct cgaatgtcat gatcatcaat caagatctgg ccttcataaa
    20761 attcgtaaaa ccgcatcaat acgttgatga tcgagctttt tccgcttccg gtgtgcccca
    20821 ctaatgcaac ggtctctccc ggattagcga caaaagagat attgttcaat acattgtgct
    20881 ctccatcata agaaaagctt acattgcgaa actcgatctt acctgaagta atcgcttgat
    20941 ctgctccagg attttgttgc ggtgcatatt cttctgtatc catgattttc aaaatacgac
    21001 taccggcaac cattccatct gtaaaggtgc tcaaaaagtc catcatttga gacattggat
    21061 taaaaaatcc ctggacatag gtcgtaaatg catagatcag tccaacttct acgggagaat
    21121 gcagcgcatc gatcccaaac aaagtcaacg ccaacgcaat cgccaatgca tatagaaaat
    21181 tgatgatcgg acttaacaga atcgaatttg ttttgatcat cgcatagcgc gtgtctaaat
    21241 actcgttgtt cacttgttca aattctttag ctaaacgagc ttcttggcga aactgctgga
    21301 taatacgcat gccggaaatc gattcattca atttcgtgtt cagctgactt aatttttccc
    21361 gcatattccg gtaaatacga gaactgaatt tctggtaata ccagatgacg attcctaaaa
    21421 tcggcaagaa caccaaagtc cacaaggcaa tttctccatt gatttgaaac atggcaatga
    21481 aggaagaaac gactgcaaag atcccagtca ataccatcaa aaagacatac caaaattcaa
    21541 atagcgtttc tgtgtcattt gtcactcgag aaacagtcga tcctgccggt gtctgatcaa
    21601 aataacgcat acctagtgta tgcagctttt caaataatct tacccgaaca tactgatagg
    21661 tcttcaatga agccatggaa tacaaatacc attggaaaaa ccaaatgata cttttgacta
    21721 tgactccaaa caagtacata ccagcaaaaa acaaaataac ttgtgtcgta gccgactgtt
    21781 tagccagatg atcatccata aaagtttgga tgatccttgg caataggaca ttgatcacag
    21841 acagggcaaa cgcaaataag atagcaacga tgaacgttct tctaaaaggt ttggcgaact
    21901 tcatcagtcg tttgatgatc ttcttctgtt ctttaagtgg aatcgatttt gtccattccg
    21961 attgataatt ttcatccatc aggcctcgcc tccttctatc ttcgcttcta atttctgttt
    22021 ctcccacatt cgttcatacc agccgcctaa tgcaagcagc tcatgatggg ttcctcgttc
    22081 aatgattttc ccttgatcga caacaatgat ttcttttgca tgcatcacgc tgcttaaacg
    22141 gtgagctgca atgatggttg ttttctcttt tcttatttct tttaaattat gcaagatagc
    22201 ttcttctgtt ttggcatcta ctgcagaaag tgcatcatct aagatcagca attcaggatt
    22261 tttgatcaat gctcgagcaa tcgaaatcct ttgtttctgt ccgccagata aagagactcc
    22321 acgttctcca actaaggtat cataaccttt tggtaattcc ttgatttcat tgttgataaa
    22381 tgctaaagaa gccgcatgct ctacttcttc ttctgaaagt tttggattag aaaaacggat
    22441 attatctcga atggtcatcg aaaacaagaa gtgatcttga ggcacatagc cgattgatcc
    22501 taataaagca ttcaatgaat attctttgat cgaacatccg ccaaattgaa tttttccttg
    22561 ataatcatcg tattcacgca tcagtaattt gatgattgtc gtttttcccg caccagtttt
    22621 cccaacaata cctaatgttt ctccttcttg gatcgcaaat ttgatttgtt ctaatgctgg
    22681 tgattcttct ttcggatagt taaaagagga aatagctaca ttcagctcgc cttttgccgg
    22741 agtctgcact gcacccagtt gttccacgat atgcgtcttt tcatgcaaaa gttcattgac
    22801 gcgatcataa gaagcatttc ctctttccaa tacgttgaac aatcgaccaa tcgcaaacat
    22861 cggccaaaca agcataccga tatagctaat aaatgagacc atctgcccga ttgagatcgt
    22921 gccttccaag acatactttc cacctaaaat aattgttgca acataagaca gaccgataat
    22981 caaggtaatg aatggatcaa ataaggagtc taaaaaattg actcgtttgt ttttaataat
    23041 tgcatcttca atcttttctt caaaatccgc gagatcttct ttttcttgtc caaatgtttt
    23101 aatgactttg atcccagtaa tactttcttg tgtcttatca ttgatactag aaaaagcttc
    23161 ttgcgaatca cgaaaagcat cgtggagttt tgaccccagc actcgtgaag taacggctag
    23221 taaaggcaga ggaatcagcg caatcagtgt taagcgccaa tcaataaaca aaatcatcgc
    23281 aatgatcgtt gttccgcccg tgatcactga atctgcaaag gtcaagatac cagctcctgc
    23341 tacattttgg attgcattca aatcattcgt tgcatgagcc atcaaatctc cagtccgata
    23401 tttttggtaa aagagattgt ccatttctgt aaaatgtttg aatagctgct gccgaagttc
    23461 tttctccaac cgagctgcac ttccccaaat attcgtccgc cagatataac gaaaaagata
    23521 ttgtaagaga gctgcaagga tcaataataa gatccaccag gaaatgatct ttattgataa
    23581 atttttatcg gcgatctcat caatgatgat cccaatcact tttggaggag ctaactgtac
    23641 caaggccact gcgaataggg agaaaatccc aattaaataa tgtttctttt cctgtcgaaa
    23701 aaaccaacct aattttctaa aaatcgacat tttactcctc ctttgtcttt atcaagagca
    23761 taggattgtc tgtgcttgtc ttttttgctg aatagtctga atagtctaac aaattacgaa
    23821 ataaaaaacg gactgaaaaa atcagcccgc aaaaagtctt tgggtcagaa cctatttttt
    23881 cccttggttc ttcatttgct gcatcatctg gcgaattttc ttctcagatg gtttttgccc
    23941 cattgacatc atcattgatc gcaacatgtc ctcattaacg ggaggatttt tttctaaata
    24001 gtctttcata tatttacgcg ctaggaaaaa gccacctact gcgccgatta gcaaagcaat
    24061 aatagcaata agtacgacaa tacctgttga taccatataa atctctcctt tctatttccg
    24121 aattgctctc aatgatttat tttactaaag tttattgaaa aaagaaaggc gtattcttta
    24181 gtttcatgca aaaaatagtg gtttttttag agaagaacaa aatttctttc attaaccagc
    24241 aagacacgcc acgaaaaaaa aattaggcat accaaatctt tttctttaat tttttctttc
    24301 tgaggtgtag aatgtaatcg tagataaata ggaaaggtgg ttttttcttg aataatcaat
    24361 ccaacaaaca atttcataag catcgactta ttgaacatac aaatggttta tctttagctg
    24421 aagtaaatgg aacagtggat gtacccaagg gaaaaagttt ttttaaaaca cttcttgctt
    24481 actctggacc aggtgcatta gtagcagttg gttatatgga tccaggtaac tggtccactt
    24541 cgatcactgg tggacaaaat ttccaatatt tattgatgtc agttattttg atgtctagtc
    24601 tgattgctat gcttttacaa tatatggcag caaaattagg aatcgtgacc caaatggatt
    24661 tggcccaagc aatccgagca cggacaagta agacattggg tgtcatttta tggatcttga
    24721 ctgaattagc cattatggca acagacatcg cagaagtcat tggtgcagca atcgccctct
    24781 acctgctttt tgatattcca ttggtaattg ccgtatttat cactgtgtta gatgttttcg
    24841 tccttttatt gctgacaaaa gtcgggtttc gtaaaatcga agctttggtt gtctgcttga
    24901 ttttcgtcat tttattcgtt ttcgtctatc aagttggctt gtctaaccct gattggcaca
    24961 gtttgtttgc cggatttatc cctaacagtc acacattctc agactcaccg gtcatcggcg
    25021 gcgaaactcc gctgactgga gctttaggga ttattggtgc aacagtcatg ccgcataact
    25081 tatatcttca ttcagcagtt tctcaaactc ggaagatcga tcaccaagac gaagaagatg
    25141 tagcacaagc ggtacgcttt tctacttggg attctaatat ccagttgtca atggcttttc
    25201 tagtcaatgc actgctattg atcatgggtg tggctgtatt caaaaccggt tctgtccaag
    25261 atccttcctt ttttggtctg tatgaagctt tatctgatcc atcagctatg agcaatggta
    25321 tcttgattgc tgtagcaaaa tctggtatct tgtctacttt atttgctgta gcacttcttg
    25381 cttctggcca gaactctact attactggta cgttgaccgg tcaagtcatc atggaaggct
    25441 tcatccatat gcgtatccca atttggctaa gaagactagt cacacgattg ctttctgtga
    25501 ttcctgtatt gatttgtgtt ttgtttacaa gtacaaaagg aacgatcgaa gaacataccg
    25561 ctttgaataa cttgatgaat gaatcccaag tttttctggc ttttgcattg ccattctcga
    25621 tgattccttt actgatgatg accaatagcc aagcggagat ggggagccga ttcaaaaatt
    25681 catttgttgt aaaactactt ggctggttct ctgtgattag tctgacttac ttgaatttac
    25741 gaggattacc aggtcaaatc gaaagtttct tcggcgagca agccacctca gctcaactag
    25801 cattggctga tcaaatcgct tatgtaatca ttgttcttgt ccttctcctg ttggcatgga
    25861 cgattggtga tttatataaa ggaaacaaaa aatatgaaca gcgtttgcag gaaacgatag
    25921 aagaaatcga gtagaaactg attcttttac acaaaggagg ttgtgtcaga agtgggcagc
    25981 ttcaagaaat aagaaggaat ttaagaaaat tgcttttcaa atttttgtga aatttcggct
    26041 tatttccgaa gaagttactt ctggaacacc gtgtatcaga ggttgtgaaa aagctgcctt
    26101 gcatcaagta ataagaacag ccaagtaaaa acagcttttt atgtttttaa cttggttgga
    26161 cttattaccg cagatgcaag cttttgagca ccgtttattc ggaaaaagga actgcgacaa
    26221 gacttttgtc acagttccct ttcctacgat tagtcaagta aaaggtgtaa gaaaaatagc
    26281 tcataaacga aagcgacaaa atcaa
//
LOCUS       DAYYHX010000016        26238 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000016 DAYYHX010000000
VERSION     DAYYHX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.016"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..673)
                     /gene="gpmA"
                     /locus_tag="ACHA6Y_000484"
     CDS             complement(<1..673)
                     /gene="gpmA"
                     /locus_tag="ACHA6Y_000484"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010497246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8986602.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAK"
     gene            complement(845..1426)
                     /locus_tag="ACHA6Y_000485"
     CDS             complement(845..1426)
                     /locus_tag="ACHA6Y_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8986603.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1559..2242)
                     /gene="rpiA"
                     /locus_tag="ACHA6Y_000486"
     CDS             complement(1559..2242)
                     /gene="rpiA"
                     /locus_tag="ACHA6Y_000486"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM8986604.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2561..2974
                     /gene="rpsL"
                     /locus_tag="ACHA6Y_000487"
     CDS             2561..2974
                     /gene="rpsL"
                     /locus_tag="ACHA6Y_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM8986605.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3058..3528
                     /gene="rpsG"
                     /locus_tag="ACHA6Y_000488"
     CDS             3058..3528
                     /gene="rpsG"
                     /locus_tag="ACHA6Y_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM8986606.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3622..5709
                     /gene="fusA"
                     /locus_tag="ACHA6Y_000489"
     CDS             3622..5709
                     /gene="fusA"
                     /locus_tag="ACHA6Y_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM8986607.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5881..7068
                     /gene="tuf"
                     /locus_tag="ACHA6Y_000490"
     CDS             5881..7068
                     /gene="tuf"
                     /locus_tag="ACHA6Y_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8986608.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7475..7783
                     /gene="rpsJ"
                     /locus_tag="ACHA6Y_000491"
     CDS             7475..7783
                     /gene="rpsJ"
                     /locus_tag="ACHA6Y_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM8986609.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7814..8443
                     /gene="rplC"
                     /locus_tag="ACHA6Y_000492"
     CDS             7814..8443
                     /gene="rplC"
                     /locus_tag="ACHA6Y_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM8986610.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8471..9094
                     /gene="rplD"
                     /locus_tag="ACHA6Y_000493"
     CDS             8471..9094
                     /gene="rplD"
                     /locus_tag="ACHA6Y_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM8986611.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9094..9384
                     /gene="rplW"
                     /locus_tag="ACHA6Y_000494"
     CDS             9094..9384
                     /gene="rplW"
                     /locus_tag="ACHA6Y_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM8986612.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9423..10256
                     /gene="rplB"
                     /locus_tag="ACHA6Y_000495"
     CDS             9423..10256
                     /gene="rplB"
                     /locus_tag="ACHA6Y_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM8986613.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10299..10577
                     /gene="rpsS"
                     /locus_tag="ACHA6Y_000496"
     CDS             10299..10577
                     /gene="rpsS"
                     /locus_tag="ACHA6Y_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM8986614.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10600..10947
                     /gene="rplV"
                     /locus_tag="ACHA6Y_000497"
     CDS             10600..10947
                     /gene="rplV"
                     /locus_tag="ACHA6Y_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM8986615.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            10961..11617
                     /gene="rpsC"
                     /locus_tag="ACHA6Y_000498"
     CDS             10961..11617
                     /gene="rpsC"
                     /locus_tag="ACHA6Y_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM8986616.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11620..12054
                     /gene="rplP"
                     /locus_tag="ACHA6Y_000499"
     CDS             11620..12054
                     /gene="rplP"
                     /locus_tag="ACHA6Y_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM8986617.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12044..12232
                     /gene="rpmC"
                     /locus_tag="ACHA6Y_000500"
     CDS             12044..12232
                     /gene="rpmC"
                     /locus_tag="ACHA6Y_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM8986618.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12257..12523
                     /gene="rpsQ"
                     /locus_tag="ACHA6Y_000501"
     CDS             12257..12523
                     /gene="rpsQ"
                     /locus_tag="ACHA6Y_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM8986619.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12581..12949
                     /gene="rplN"
                     /locus_tag="ACHA6Y_000502"
     CDS             12581..12949
                     /gene="rplN"
                     /locus_tag="ACHA6Y_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM8986620.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            12986..13294
                     /gene="rplX"
                     /locus_tag="ACHA6Y_000503"
     CDS             12986..13294
                     /gene="rplX"
                     /locus_tag="ACHA6Y_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM8986621.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13322..13861
                     /gene="rplE"
                     /locus_tag="ACHA6Y_000504"
     CDS             13322..13861
                     /gene="rplE"
                     /locus_tag="ACHA6Y_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM8986622.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13880..14065
                     /locus_tag="ACHA6Y_000505"
     CDS             13880..14065
                     /locus_tag="ACHA6Y_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM8986623.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14101..14499
                     /gene="rpsH"
                     /locus_tag="ACHA6Y_000506"
     CDS             14101..14499
                     /gene="rpsH"
                     /locus_tag="ACHA6Y_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM8986624.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14531..15067
                     /gene="rplF"
                     /locus_tag="ACHA6Y_000507"
     CDS             14531..15067
                     /gene="rplF"
                     /locus_tag="ACHA6Y_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM8986625.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15228..15584
                     /gene="rplR"
                     /locus_tag="ACHA6Y_000508"
     CDS             15228..15584
                     /gene="rplR"
                     /locus_tag="ACHA6Y_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM8986626.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15605..16105
                     /gene="rpsE"
                     /locus_tag="ACHA6Y_000509"
     CDS             15605..16105
                     /gene="rpsE"
                     /locus_tag="ACHA6Y_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM8986627.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16120..16299
                     /gene="rpmD"
                     /locus_tag="ACHA6Y_000510"
     CDS             16120..16299
                     /gene="rpmD"
                     /locus_tag="ACHA6Y_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM8986628.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16343..16783
                     /gene="rplO"
                     /locus_tag="ACHA6Y_000511"
     CDS             16343..16783
                     /gene="rplO"
                     /locus_tag="ACHA6Y_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM8986629.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16783..18078
                     /gene="secY"
                     /locus_tag="ACHA6Y_000512"
     CDS             16783..18078
                     /gene="secY"
                     /locus_tag="ACHA6Y_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM8986630.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18140..18787
                     /locus_tag="ACHA6Y_000513"
     CDS             18140..18787
                     /locus_tag="ACHA6Y_000513"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM8986631.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            18978..19196
                     /gene="infA"
                     /locus_tag="ACHA6Y_000514"
     CDS             18978..19196
                     /gene="infA"
                     /locus_tag="ACHA6Y_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM8986632.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19229..19345
                     /gene="rpmJ"
                     /locus_tag="ACHA6Y_000515"
     CDS             19229..19345
                     /gene="rpmJ"
                     /locus_tag="ACHA6Y_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM8986633.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19363..19728
                     /gene="rpsM"
                     /locus_tag="ACHA6Y_000516"
     CDS             19363..19728
                     /gene="rpsM"
                     /locus_tag="ACHA6Y_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM8986634.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19756..20145
                     /gene="rpsK"
                     /locus_tag="ACHA6Y_000517"
     CDS             19756..20145
                     /gene="rpsK"
                     /locus_tag="ACHA6Y_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM8986635.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20225..21163
                     /locus_tag="ACHA6Y_000518"
     CDS             20225..21163
                     /locus_tag="ACHA6Y_000518"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM8986636.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21191..21571
                     /gene="rplQ"
                     /locus_tag="ACHA6Y_000519"
     CDS             21191..21571
                     /gene="rplQ"
                     /locus_tag="ACHA6Y_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM8986637.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21843..22682
                     /locus_tag="ACHA6Y_000520"
     CDS             21843..22682
                     /locus_tag="ACHA6Y_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8986638.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22658..23527
                     /locus_tag="ACHA6Y_000521"
     CDS             22658..23527
                     /locus_tag="ACHA6Y_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8986639.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23517..24314
                     /locus_tag="ACHA6Y_000522"
     CDS             23517..24314
                     /locus_tag="ACHA6Y_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8986640.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24517..25263
                     /gene="truA"
                     /locus_tag="ACHA6Y_000523"
     CDS             24517..25263
                     /gene="truA"
                     /locus_tag="ACHA6Y_000523"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8986641.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            25927..26121
                     /locus_tag="ACHA6Y_000524"
                     /pseudo
     CDS             25927..26121
                     /locus_tag="ACHA6Y_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         8530 a         4830 c         5628 g         7250 t
ORIGIN      
        1 ttttagcaac tgttaagtca tcatttaatt cataaacaag tggttgacct gttggaattt
       61 caagatccat gatgtcttcg tcagagatac cttcgatatg tttagctagg gcgcgtaatg
      121 agttaccatg tgcagctact aaaacagttt tgttgtctag taatgctgga gcaatttcat
      181 cttgccagaa tggcaaagcg cgttctaatg tcactttcaa gttttctcca ccaggaatat
      241 cgcgtttgtc taacattgcg taacgacggt catttgctgc agaaccttcg tcagtttctt
      301 ccattaatgg aggaagagta tcgtaagaac gacgccagat gtgcacttgt tcgtcgccgt
      361 atttttcagc tgtttctttt ttgttcaaac cttgtaattt accatagtga cgttcgttta
      421 agcgccatga tttgatttgt ggtacccaaa gttgatctga atattctaag atcaagttac
      481 atgttttgat tgcacgagtt aaaacagaag tgtaagcaac atcaaattcg attccagctt
      541 ctttgatttt gcgtccgcct tcttttgctt cttcgattcc ttctggtgct agatttacat
      601 cagcccaacc agtaaattgg ttcaatgcat tccattcact aagaccgtga cgagaaaaaa
      661 ctaattttgg catgtttagc tctcctttta agataggttt atatttctga aattttcagt
      721 tctgttttat tgtacaattt tttggaagag atttccatcg aaaaattaga taagattcta
      781 tgagaaaaca agtagagaat ataaaaaaac gaacagtgcc cacgcaactg ttcgttttta
      841 ggtattagat gcataattgc tcgatttctt tttcaggtaa attgatatat ttataacatg
      901 ttccaactga aacaccgcat tttgaagaaa taaattggat cgtttctttt ttttcataat
      961 agagacgtcg gatttttttg atcgtttttg catcgatttt tggtcttccg ccgatttttc
     1021 cttttttacg agcttcatct aatccaacta atgtacgttc tttgatcaat gcgcactcca
     1081 tgtttgctag gttttccatt aattggaaat agatttttcc catttcgcgc gtatcgattc
     1141 cttcatcaag actaatcaag tgcagcccgc gatctttgaa taattgcgtg agttctgtta
     1201 attgccgcgt agatttgcct aatcgatgaa gttggcaaat gactagtgca tcgccttgtt
     1261 gcatgtcagc gatcactgac tccaaatgaa cagcagtaga atcattttgt tccacttcat
     1321 atgtctcatg gaaaatttct tgacaaccaa agtcagataa ttgatcaacc tgagttgtta
     1381 aatcaacgtc atttatggtt gtgcgtgcat aaccaataat tttcatggtg attcctttct
     1441 agcagtgatt ttctaactgg atataacagt aaaaatgtag cataaagtac gtcaaaaaga
     1501 tagttttttc gcaaattttc cgctgtcttt ttttattgga tgatcaagca gtttttcttt
     1561 atttacgacg gattgcgtca agaatctcaa cgcctttttc tgagccgaca actactttag
     1621 aaaccatgtt taggaaaagc ccatgttcta ctaccccaac aagttcatct aaataggtag
     1681 ctaagctttc tggctgttcg atgacttcta gatgcagatc aatgatgtaa tgaccaccat
     1741 ctgtggttaa gacttctccg ttttcgtctg tacgcaagac gggttggaat cttttttctt
     1801 caaaaatatg aagtagctgt tgggagccat aagggatcac ttcaactggg agtgggaact
     1861 tcccaagttt atcgactaat ttagaagaat caacgatcca aatcgtttct tttgagtacg
     1921 tagcgactat tttctcgaaa agcaaagcag cgccgccgcc tttgatccct tgaaaatcag
     1981 cactgatttc atcagcaccg tctattgtca aatcgactac ggggacttca tcaatactct
     2041 ttaacggtat tcctaatttt tccgcttgtt cttttgtcgc atttgaagta gtaactcctg
     2101 tgatacgtaa accttcttca cgcatacggc gaccaatctc ttctaccata taataggcgg
     2161 ttgaaccggt cccgagacct acgatcatcc cgtcctttac gaatttcgcg gcttcaatgc
     2221 cagctaattg ttttaaattc atctttgcgt ccaccttttt ttagtttgag catcgtgctt
     2281 tcctcactat tttagcgtga tttacggtaa aaagatacca ttctttttta ttccaataaa
     2341 gctttttcaa aaaaaattgc atctcttgtt gacatgtttt ttcattcgtg atattctaac
     2401 aaaggtgctt tatctacagg tttctgtgaa gaaacgtgct gactgtttat caaagcatat
     2461 ttgataagga tgcagacatt gcatttattt tttatcacaa aaaaagaacc acctggatgt
     2521 gtggaactag acgcaaatcg tgggaaggag gaaacaagga atgcctacaa ttaaccaatt
     2581 agtacgtaaa cctcgtaaat caaaggtgga aaaatctaat tcacctgctt tgaacaaagg
     2641 atataacagc tttaagaaaa ctcaaactaa cgtaaactca cctcaaaaac gtggggtagc
     2701 aactcgtgtg ggtacaatga cacctaaaaa accgaactca gctttacgta aatatgcacg
     2761 tgttcgtttg tcaaacttaa ttgaagttac tgcttatatc ccagggattg gtcataactt
     2821 acaagaacac agtgttgtat tattacgcgg tggtcgtgta aaagacttac caggggtacg
     2881 ttaccatatc gttcgcggtg ctttagatac tgctggtgtt aacgaccgta aacaaagccg
     2941 ttctaaatac ggaactaaaa aacctaaagc ttaattttaa ttattaaaca aatgaaactt
     3001 gcttctacta tatagaagta attactatga acaatatttc ggaaggagga gttacggatg
     3061 ccacgtaaag gtcctgttgc aaaacgtgat gttttaccag atcctattta taactcaaaa
     3121 ttagtaactc gcttgatcaa ccgtgtaatg gttgacggaa aacgcgggat tgcagctaac
     3181 attatctata atgcctttga cttgatcaaa gaatctacag gtaacgatcc attagaagtg
     3241 tttgaacaag caatgaaaaa cgttcaacct gttcttgaag ttaaagcacg tcgtgttggg
     3301 ggttcaaact accaagtacc agttgaagtt cgccctgaac gccgcgttac tctaggtctt
     3361 cgttgggtag taaactacgc tcgcttgcgc ggtgaacaca caatggaaga acgcttagca
     3421 aaagaaatca tggatgctgc aaacaacaca ggtgcttctg ttaaaaaacg tgaagacact
     3481 cataaaatgg ctgatgccaa ccgtgcgttt gctcactatc gctggtaaga ttctctctgt
     3541 ttggtcggct tttataagtc gacctcagat agtgtcctta tttttagcaa ctagatagta
     3601 aagagaggag aaacaaggag aatgaccaga gaattttctt tacaaaacac tcgtaatatc
     3661 ggtatcatgg cccacatcga tgctggtaaa acgactgcta ctgaacgtat cttgtactat
     3721 actggtaaaa tccataaaat tggtgaaact catgaaggag cttcacaaat ggactggatg
     3781 gaacaagaac aagaacgtgg tattacgatc acttctgctg ctactacagc acaatggaag
     3841 ggtcaccgaa tcaacatcat cgacacacca ggacacgtag acttcacagt tgaagttgaa
     3901 cgttcattgc gtgtacttga tggtgctgta actgttcttg atgcacaatc aggtgttgaa
     3961 cctcaaactg aaacagtttg gcgtcaagca acaacttatg gtgtacctcg tatcgtattt
     4021 gcaaacaaaa tggacaagat cggtgctgac ttcttatatt cagtatcaac tttacatgac
     4081 cgtttgcaag caaacgctca cccaatccaa ttaccaattg gtgcggaaga cgacttcaca
     4141 ggaattatcg accttgtaac aatgaaggct gaaatgtata caaacgatct tggaacagag
     4201 atcgaagaaa cagaaattcc tgaagaatac cgcgaattag ctgaagaatg gcgcgaaaaa
     4261 ttggttgaag ctgtagctga aactgatgaa gaacttactt tgaaatactt agaaggtgaa
     4321 gaaatcactg aagctgagtt aaaagaaggt atccgccgcg caacagttaa cgtagaattc
     4381 tacccagtac tatgtggttc agcatttaag aacaaagggg ttcaattact attggacgct
     4441 gttcttgatt acttgccatc accgttagat atcccagcta tcaaagggat tgaccctaaa
     4501 actgatgaag aagtagaacg tcctgcagac gacagcgctc ctttctcagc tttagcattt
     4561 aaagttatga ctgacccatt cgtaggtcgt ttaactttct tccgtgtgta ctctggtgtt
     4621 ttacaatcag gttcatacgt acaaaacgca acaaaaggca aacgtgaacg tgttggtcgt
     4681 atcctacaga tgcatgccaa ctctcgttca gaaatctctg aagtttacgc tggagatatc
     4741 gctgctgctg ttggtttgaa agacacaact actggggaca cactatgtga tgaaaagagt
     4801 ctagttattc ttgaatccat ggaattccca gaaccagtta tccaagtcgc tattgaacct
     4861 aaatcaaaag ctgaccaaga taaaatgggt gtggctttac aaaaactatc tgaagaagat
     4921 cctactttcc gtgctgaaac aaacgtggaa acaggcgaaa caatcatcgc tggtatgggt
     4981 gaacttcact tagatatcat cgttgaccgt atgcgtcgtg aattcaaagt tgaagctaac
     5041 gtaggtgcgc ctcaagtatc ttaccgtgaa actttccgtg ctggcactca agccgaaggt
     5101 aagtttgtac gtcagtctgg tggtaaaggt caatacggtc acgtatggat tgaatttaca
     5161 ccaaatgaag aaggtgctgg tttcgaattt gaaaatgcga tcgtcggtgg ggttgttcct
     5221 cgtgaataca ttccagctgt tgaaactggt ttgaaagatg ctatggaaaa tggtgtctta
     5281 gctggctatc cattagtaga tatcaaagct aaactttacg atggttcata ccatgatgtc
     5341 gattcaaatg aaactgcctt ccgtgttgct gcttctatgg cattacgtgc agcagctaag
     5401 aaagctaatc cagtaatcct tgaaccaatc atggctgtag aagttgttat ccctgaagat
     5461 tatttaggtg atgttatggg tcacgtaact gctcgtcgtg gacgtgttga aggtatggaa
     5521 gctcgcgctg gcggccaaca agttgttcgt gcgatggttc cacttgctga aatgtttggt
     5581 tatgctacaa ctcttcgttc tgctactcaa ggtcgtggta catttacaat gacatttgac
     5641 cactacgaag atgtaccaaa atcagtacaa gaagaaatca ttaagaaaaa cggcggaaaa
     5701 gctgaataat ttcttaaaac cggatagtcc tgcaaagaga ttatccaaag agactatatt
     5761 tttaatatag tttcgtgtaa aataattatc gatgatatgg gcatgtggtg taccttgtgt
     5821 acatcatata cctatcagac aaataaaaaa tatattgtat ttttaggagg acattttaaa
     5881 atggcaaaag aaaaatttga ccgttctaaa ccacacgtta acattggtac tatcggacac
     5941 gttgaccatg gtaaaactac attaacagct gcaatcacaa ctgtactatc taagaaaaac
     6001 ggcggccaag ctatggctta cgatcaaatc gatggtgctc cagaagaacg cgaacgcgga
     6061 atcacaatct caacagctca cgttgagtat gaaacagata cacgccacta cgctcacgtt
     6121 gactgcccag gacacgcgga ttacgttaaa aacatgatca ctggtgctgc tcaaatggac
     6181 ggagctatct tggtagtttc tgctgctgac ggcccaatgc ctcaaactcg tgaacacatc
     6241 ctattgtctc gtcaagttgg tgttccttac atcgttgtat tcttgaacaa agtagacatg
     6301 gttgatgacg aagaattact agaattagtt gaaatggaag ttcgtgacct attaacagaa
     6361 tacgaattcc ctggtgacga tgttcctgta gttgctggat cagctttgaa agctctagaa
     6421 ggcgacgctt catacgaaga aaaaattctt gaattaatgg ctgcagttga cgaatacatc
     6481 ccaactccag aacgtgacaa cgacaaacca ttcatgatgc cagttgaaga cgtgttctca
     6541 attactggac gtggtactgt tgctacaggt cgtgttgaac gtggacaagt tcgcgttggt
     6601 gacgaagttg aagttgttgg tattgctgaa gaaacttcaa aaacaacagt tactggtgtt
     6661 gaaatgttcc gtaaattgtt agactacgct gaagctggag acaacattgg tgctttacta
     6721 cgtggtgttg cacgtgaaga catccaacgt ggacaagttt tagctaaacc aggtacaatc
     6781 acacctcata caaaattctc tgcagaagta tacgtgttga caaaagaaga aggtggacgt
     6841 catactccat tcttcactaa ctaccgtcca caattctact tccgtacaac tgacgtaaca
     6901 ggtgttgttg aattaccaga aggaactgaa atggttatgc ctggcgacaa cgtaacaatg
     6961 gaagttgaat taatccaccc aatcgctatc gaaaacggta ctaaattctc aatccgtgaa
     7021 ggcggacgta cagttggtgc cggtgtcgtt acagaaatca aagcttaatt cacttaagtt
     7081 ttaaagtaaa gcaacattta ttcggacgta atcgctcgga acgaaggtct taggatcttt
     7141 gttccgagct tttttattta ttgcaggatc aggaaaaaag acaggagtat tataaataga
     7201 agaaacacga aataaaagag tcagtatttt ctaaatgaaa ctttatgaaa acaaaatgtt
     7261 ttttacaaga aaaagccttg ctttgtgagt ttatatatat tataatgaca aaggtgcgtt
     7321 tactttagtg aattgcacag acgtaatcag aatgatttcg tcggctaaga aacgagaggt
     7381 tgcgacacgc ccggacgctt tgccacgaac gagcgtgacg gaaattttcg tggagctatg
     7441 tctacttttc aaaataggcg aaggagggaa caagatggca aaacaaaaaa ttcgtatccg
     7501 tttaaaagcg tatgaacacc gtattttaga tcaatcagcg gataaaatcg tggaaactgc
     7561 aaaaagaact ggagctagcg tgtctggtcc aattccatta ccaactgaac gcagtcttta
     7621 cacaatcatc cgtgcgactc ataaatacaa agattcacgc gaacaattcg aaatgcgtac
     7681 acacaaacgt cttatcgaca ttgtgaaccc aacaccaaaa actgttgatg ctttgatgaa
     7741 gctagactta ccatctggtg taaacatcga aatcaaatta taaaactaaa acaaaaataa
     7801 tggaggtgta ctcatgacca aaggaatctt agggaaaaaa gtgggaatga cacaaatctt
     7861 cactgaatct ggtgaattaa ttccagtaac tgttgtagaa gctactccaa acgtagtact
     7921 acaagtgaaa acaatggaaa cggatggcta cgaagctatt caagttggtt accaagatat
     7981 gcgtgaagtt ttatcaaaca aacctgcgaa aggtcatgtt gcaaaagcaa acacggctcc
     8041 taagcgcttc attcgtgaat tcaaaaatgt tgagctagga gaatatgaag taggaaaaga
     8101 aattacagta gatgttttcc aagcaggaga cattattgat gtaacaggta ctacgaaagg
     8161 taaaggattc caaggggtta tcaaacgcca cggacaaagc cgcggaccaa tggctcacgg
     8221 ttctcgttac catcgtcgtc ctgggtcaat gggtccagtt gcacctaacc gtgtatttaa
     8281 aaacaaacgt cttgctggcc gtatgggtgg caaccgcgta actatccaaa acttggaagt
     8341 tgtacgtgtc gatgcagaaa gaaatgttat cttgatcaaa ggtaatgttc ctggagctaa
     8401 aaaatcttta attactatca aatcagctgt gaaagctaaa taattgtagg aagaaaggag
     8461 gaactaagga atgccgaatg tagcattatt caaacaagat ggaagccaaa acggtgaaat
     8521 cacacttaac gaagaaatct tcggaattga acctaatgaa agtgttgttt acgatgcaat
     8581 cgtaatgcaa cgtgcgtcat taagacaagg aacacacgcg gttaaaaacc gtagcgctgt
     8641 tcgcggcggc ggacgtaaac catggcgtca aaaaggaaca ggtcgtgcac gtcaaggatc
     8701 tatccgctca ccacaatggc gtggaggtgg cgtagtcttt ggaccaacac cacgttctta
     8761 cagctacaaa cttcctaaaa aagttcgtcg tttagcaatg aaatctgtat tgtcagaaaa
     8821 agttgctgaa aacaacttgg tagcaatcga aggcttgaac ttcgacgcac caaaaacaaa
     8881 agaattcaaa caagttcttg ctaacttatc tatcgactct aaagtattgg ttgttctaga
     8941 aaaaggtaac gactttgctg ctttatcagc acgtaactta ccaaacgttt ctgtagtaac
     9001 ttctgataat gtgagtgtgc tagacgttgt atcaaataca aaagtgttag caacacaaac
     9061 tgctcttact caaattgagg aggtgcttgc ataatgaact tactagacgt aatcaaacgc
     9121 ccagtgatca ctgaaaaatc aatgcttgcc atggatgaca agaaatacac ttttgaagtg
     9181 gacactcgcg caaacaaaac tttagtaaaa caagcggtag aagctgcttt tgacgttaaa
     9241 gtcaaaaacg tgaacatcgt gaacgtacgt gcgaaattca aacgtatggg caaatatgca
     9301 ggatatacaa aaaaacgtcg caaagcgatc gttacattga ctgaagattc aaaagaaatc
     9361 caattattcg aagctgctga ataagcacta atagataact cattaaatta ggagggaaaa
     9421 acgtggcgat taaaaagtac aaacctacct caaatggtcg tcgtaacatg acaggttctg
     9481 attttgctga aatcacaaca tcaacacctg aaaaatcatt gttacaacca ttgaaaaacc
     9541 atgctggacg taacaacaac ggccgcatca ctgtacgtca tcaaggtggc ggacacaaac
     9601 gccaatatcg cgtgatcgac ttcaaacgta acaaagataa cgttgttgca actgttcaaa
     9661 cgatcgaata tgatccaaac cgttcagcta acatcgcgtt agtacactat gaagatggag
     9721 tgaaagctta catcttagca cctaaaggac tagaagtggg tatgaaactt gtttcaggtc
     9781 cagaagcaga tatcaaagtc ggaaatgctc ttccattaga aaacattcca gttggtactg
     9841 ttatccacaa cattgaaatg aaacctggta aaggtggaca attgatccgt tctgctggta
     9901 caagtgctca agtacttggt aaagaaggca aatacgtatt gatccgcttg aactctggtg
     9961 aagttcgtat gatcttagct gcatgccgtg ctacaatcgg ttctgtaggt aacgaacaac
    10021 acgaattaat caacatcggt aaagctggac gctctcgttg gatgcgtaaa cgcccaactg
    10081 tacgtggtag cgtaatgaac cctaatgatc acccacacgg tggtggtgaa ggtaaagctc
    10141 ctatcggacg taaagcacca gtttctcctt ggggtcaacc agctcttggt tacaaaactc
    10201 gtaacaaaaa agctaaatca gacaaactta ttgttcgtcg tcgcaaaaat aaataattac
    10261 tttgaccatg tgtcgcacat tttgagagga ggttcaccat gggtcgtagt ttaaagaaag
    10321 gaccttttgt cgatgagcat ttaatgaaaa aagttgaagc tcaagcaggg gttgaaaaga
    10381 aaaaagttat caaaacttgg tcacgccgtt caacagtttt cccaagtttt gttggatata
    10441 ccatcgcagt ttatgatgga cgtaaacatg taccagttta catccaagaa gacatggtag
    10501 gacacaaatt aggtgaattt gcacctacaa gaacttatcg tggacatgct gctgacgata
    10561 agaaaacgaa acgctaattt gaggggagga taaatccaaa tggcagaaca aatcacatca
    10621 gctaaagcaa ctgcaaaaac agttcgcact tcacctcgaa aagcccgttt agtaattgac
    10681 cttatcagag gtaaaagtgt cgcagatgca atttcaatct tgaaattcac accaaacaaa
    10741 tctgctggaa tcattgaaaa agtattgatg tcagcagttg cgaatgcaga aaataacttt
    10801 gacttagatg ttgaaaactt ggtagtaagc gaagcttttg ttaacgaagg acctacaatg
    10861 aaacgtttcc gtcctcgtgc aaaaggatca gcttctccaa tcaacaaacg tacaagtcat
    10921 atcacggtag tcgtatcaga aaaataagga gggataatca gtgggtcaaa aagtacatcc
    10981 aattggaatg cgtgtaggca tcatccgcga ctgggatgct aaatggtatg ctgaaaaaga
    11041 gtatgctgag ttcttacacg aagatttaag aatccgtaaa tttatttcaa ctaaacttgc
    11101 tgatgctgct gtatcaacag ttgaaatcga acgcgcaaca ggtcgcgtaa acatttcaat
    11161 tcacacagct aaaccaggta tggttattgg taaaggcggt tctgaagtcg aaagcctaag
    11221 aaaagaatta aacaaattaa ctggtaaaag aatccatatc aatatcgtgg aaatcaaaaa
    11281 accagatcta gatgcaaaat tagtaggtga aggaatcgca cgtcaattag aaaaccgtgt
    11341 tgctttccgt cgcgctcaaa aacaagccat ccaacgctca atgcgtgctg gcgctcaagg
    11401 gatcaaaact caagtatctg gtcgtctaaa cggagcagat atcgctcgtt cagaaggata
    11461 ctcagaagga actgttcctc ttcatacttt acgtgcggac attgattacg catgggaaga
    11521 agcagataca acatacggaa aactaggagt taaagtgtgg atctatcgtg gagaaattct
    11581 tccaactaaa aaaaacactg agaaaggagg gaaataatca tgttagtacc taaacgtgta
    11641 aaacaccgtc gtgaattccg cggaaaaatg cgcggggaag ctaaaggcgg aaaagaagta
    11701 gcattcggtg aatacggttt gcaagctgtt gattcacatt ggatcacaaa ccgccaaatc
    11761 gaagctgctc gtatcgcaat gactcgttac atgaaacgtg gtgggaaagt atggattaaa
    11821 attttccctc acaaatctta tactgccaaa gcaattgggg tacgtatggg ttctggtaaa
    11881 ggggcacctg aaggatgggt tgcaccagta aaacgtggta aaatcatgtt tgaaatcgca
    11941 ggcgttcctg aagaagtagc tcgtgaagcg ttacgtctag cttctcacaa attaccaatg
    12001 aaaactaaga tcgtaaaacg tgaggaaatg ggtggtgaat cgaatgaagg ttaaagaaat
    12061 cagagaatta accactgccg aaatgcttga tcaagaaaaa caattaaaag aagaattgtt
    12121 taatcttaga ttccaattag caacaggtca attagaaaac actgcacgta ttaaagaagt
    12181 acgtaaatcg attgcacgca tcaaaactgt gttgcgtgaa caagctaagt aattatggaa
    12241 ggaggccatt aagcgtatga ctgaaggaag aaatcaacgc aaagtttatc aaggtcgcgt
    12301 ggtttcagac aaaatggata aaacaattac cgttgctgtc gaaacaaaga aaaaccaccc
    12361 tatctatggc aaacgcatga actattctaa gaaatacaaa gtgcatgatg aaaacaacac
    12421 agcaaaagtt ggcgacatcg tgagaattat ggaaactcgt ccattatcag ctacaaaacg
    12481 tttccgtttg ttggaagtag tcgaagaagc agttattatc taataacacg agattttcct
    12541 atatagattg aatgcaaaat ctgaaaggag gatacacaac gtgatccaag cagaaagtcg
    12601 tttaaaagtc gctgataact caggcgcacg tgaaattttg acgatcaaag tccttggtgg
    12661 atctggtcgt aaaactgcta acatcggtga tgtgatcgtt gctacggtta aacaagcaac
    12721 gccaggtggg gttgtcaaaa aaggtgacgt cgttaaagcc gttatcgttc gaacaaaatc
    12781 aggagctcgt cgtacagacg gttcatatat taaatttgat gaaaatgctg cagtgattat
    12841 tcgtgatgat aaaagccctc gtggaacacg tatctttggg ccagttgcac gtgaactacg
    12901 tgaaaacaac ttcatgaaga tcgtttcact agcaccagaa gttttataat cttgagacac
    12961 ttatcaaagg aggtgcgaaa cagttatgtt tgttaaaaaa ggcgataaag ttaaagttat
    13021 cactggaaaa gacaaaaaca aagaaggcgt tgtactagca gcgttcccga aaaaagataa
    13081 agttattgtc gaaggtgtaa atgtcgttaa aaaacaccaa aaaccttctc aagctgctcc
    13141 tcaaggtgga attgttgaga tggaagcgcc aattcatgtt tctaacgtga tggtggttga
    13201 ctctactggt gtagctggcc gagttggcta caaagaagta gacggcaaaa aagtccgtgt
    13261 ttctaaaaaa accggtgaag ttttagataa ataaaagttt aaggaaggag ggtcttactg
    13321 aatgaaccgc ctaaaagaaa aatatcttaa agaagtaact ccatcattga tggaaaaatt
    13381 taactatagc tctgttatgc aaacacctaa agttgaaaaa atcgtcatca acatgggtgt
    13441 tggtgatgcg gtatcaaacg ctaaaaactt agataaagca gttgaagaat taactttgat
    13501 cactggtcaa aaaccaatga ttacaaaagc taaaaaatca atcgctggat tccgtttacg
    13561 tgaaggaatg ccaattggtg caaaagttac attgcgcgga gaaagaatgt acgaattctt
    13621 agacaaattg gtttctgttt cactaccacg tgtacgtgac ttccatggtg taagtaaaaa
    13681 agcctttgac ggtcgtggaa actatacttt aggtattaaa gaacaattga tcttcccaga
    13741 agtagattat gatttagtag ataaagtacg tggtatggac atcgttattg taacaacagc
    13801 aaactctgat gaagaatctc gtgagttgtt gacacaatta ggcatgccat tccaaaaata
    13861 attaaaggag gcgaactacg tggctaaaaa atcaatgatt gctaaaaaca aacgtcctgc
    13921 aaaattttca acacaagctt atactcgttg cgaacgttgc ggacgtccac attcagttta
    13981 tcgtaagttc catctttgcc gtatttgctt ccgcgaactt gcctataaag gtcaaattcc
    14041 cggcgtgaag aaagctagct ggtaaaaagt aaattcgcat aagaaggagg taaaagatca
    14101 atggtcatga cagatccaat tgcagatttt ctaactcgta tccgtaacgc aaacatggtt
    14161 aaacatgagt ctttagaagt acctgcatca aaaattaaac gtgacatcgc tgaaatcttg
    14221 aaacgtgaag gtttcgttcg tgatgttgaa tacatcgaag atgacaaaca aggcgtgatc
    14281 cgtgttttcc taaaatttgg taaaaacggc gaacgtgtta tcacaaactt aaaacgtatc
    14341 tctaaaccag gtttgcgtgc ttatgtcaaa gctgacgaag taccaaaagt attgaacggt
    14401 ttaggtatcg caatcatttc gacttctgaa ggtgttgtca ctgataaaga agctcgtgaa
    14461 aaaaatatcg gcggcgaagt tatcgcttac gtatggtaat caagtagaat ctacaaggag
    14521 gtgtctttaa gtgagccgta tcggaaataa agtcgttgtt atccctgaag gagtaacagt
    14581 tactcaagac ggaaacaacg ttacagttaa aggaccaaaa ggtgaattga ctcgtacttt
    14641 ttcagcagat atcaaaatga atatcgaagg aaacgaagta actttcactc gtccaaacga
    14701 cagtaaagaa atgaaaacaa ttcatggaac aactcgtgcg aacttcaaca acatggttgt
    14761 aggtgtcagc gaaggtttcc aaaaagcgtt agaactaatc ggggttgggt accgtgcaca
    14821 attacaagga tcaaaacttg tattgaacgt tggttactca catccagtag aaatcacacc
    14881 accagctgga gtaactgttg aagtaccagc taacacacaa gtaattgtta aaggtgcgaa
    14941 caaagaagaa gttggcgaat tagctgcaaa cattcgtgga actcgtcctc cagaacctta
    15001 taaaggtaaa ggtattcgtt atgttggcga atatgtacgc cgtaaagaag gtaaaactgg
    15061 taaataatcg tttagaaaaa ttgcatagaa gccagcagca ggaaaaaggc atttggctga
    15121 agtttacctt caaaaaaata catttttact tgtgccgcag cttctatttt tcgaaacgta
    15181 tttactgctt gaaatagtag tacaataaaa ataaagaggt gacaattgtg attacaaaac
    15241 cagataagaa caaaacacgt caaaagagac atcgtcgtgt acgtaacaat atctctggta
    15301 ctgctgagcg cccacgcttg aacgtttttc gttctaacaa aaacatctac gctcaaatta
    15361 ttgatgacgt agcgggtgta acgctagcaa gtgcctctac cttggataaa gaaatttcag
    15421 gtggaacaaa aacagaagca gcacaagctg tcggtaaact agttgctgaa cgtgcagctg
    15481 aaaaaggcat taaagtagta gtctttgacc gtggtggata cctttaccat ggccgtgtgc
    15541 aagctttagc tgaagctgct cgcgaaaatg gactagaatt ttagaagaag gaggaatacc
    15601 attcatggtt tatatcgatc caaaacattt ggaattagaa gaccgcgttg ttgcaattaa
    15661 ccgtgtaaca aaagttgtta aaggtggacg tcgtctacgt tttgctgctt tagttgttgt
    15721 cggtgacaaa aacggacacg tagggtttgg tacaggtaaa gctcaagaag ttcctgaagc
    15781 aatccgtaaa gcaattgaag atgcgaagaa aaacttagtt gaagtaccta tggttggttc
    15841 aacaatccca cacgaagtaa tcggtgcttt tagtggtggt agaatcctaa tgaaacctgc
    15901 tgtagaaggt tctggggttg ctgctggtgg accagttcgt gccgtattag aattagctgg
    15961 ggtagcagat atcacttcta aatctttagg ctcaaataca ccaatcaacg ttgttcgcgc
    16021 aacagttgaa ggtttgaaac aattgaaacg tgcagaagaa gttgcagaac ttcgcggcaa
    16081 atcagttgaa gaattaatcg gataaggagg acaacaataa tggctgaatt aaaagttact
    16141 ttaaaacgca gtattatcgg acgtcctcaa aaccaacgcg atacagttaa agcgttaggt
    16201 cttacaaaga ttaacagttc agtggttaag ccttctaatg aagcaattaa aggcatgatc
    16261 aatactgttt ctcacctagt ggacgttgaa gaaatttaat ttagaaaaat cataactaga
    16321 acattaagga ggtgccaaac ctatgaaact tcatgaatta aaacctgctg aaggttcacg
    16381 ccaagtacgt aaccgtgttg gtcgtggtac ttcatctggt aacggaaaaa ctgccggacg
    16441 cggacaaaaa ggacaaaaag ctcgttcagg cggcggtgtg cgtttaggat ttgaaggggg
    16501 acaaacacca ttgttccgtc gtttaccaaa acgaggattc acaaacgtga accgtaaaga
    16561 ctatgcagtc attaatcttg atgtattgaa ccgctttgaa gacggagctg aagtaactcc
    16621 tgttgcttta gttgaagctg gaatcgtgaa aaacgaaaaa gctggaatca aagtattagc
    16681 taatggggaa ctaaacaaga aattaactgt gaaagcagca aaattctcta aagcagctca
    16741 agaagcaatt gaagcggctg gtggatcaat cgaggtgatc taatgtttag actattaaaa
    16801 gatgctttta aagtaaaaga cattagatcg aaaatcctat ttactgtatt tgtgttgttt
    16861 gtatttcgcc taggtgcaca tattactgta ccaggcgtaa atgctgcttc attgcaaaac
    16921 ctttcttctt tgccattttt caacatgttg aacttggtaa gtggtagtgc gatgcaaaac
    16981 ttttccattt tctcaatggg ggtttcacca tacattactg cttctatcgt cattcagctt
    17041 cttcaaatgg acatcgtgcc tcgatttgtt gaatggtcga aacaagggga agttggtcgt
    17101 aaaaaactga atcaagcgac tcgatatctg acattagtat tggcatttat ccaatctgtc
    17161 ggtatcactg ctggattcaa tcaatacgct caattaggtt tcgtgaataa tcctaacgta
    17221 gcaacttacg taacgatcgg tttgattcta acagccggta cgatgtttgt tacttggcta
    17281 ggagaacaga tcacagaaaa aggtatcgga aatggcgtat caatgatcat ttttgcaggt
    17341 atcatttcta gattgcctga aagtatcaga gaaatttatg aagattactt tataaatatt
    17401 gatccttcag acatatggaa atctgctatt tttgtggcca ttttactagt agcggtcttg
    17461 ttgatcgtga cattcgtaac atttttccaa caagctgaac gcaagatccc gatccaatat
    17521 acgaaacgcg tagctggcgc gcctacaagc agctatctgc cgttgaaagt aaatgcagct
    17581 ggagttattc cagttatctt tgctagttcg tttattgcta cgccaaatgc tgttttacaa
    17641 gcattgagcg gtagattcgg ttctgaaaac tggtacaaag tgatgatgga gatctttaac
    17701 tataatacag cacctggtgc tacgatttat acgattctga tcgttgcctt cactttcttc
    17761 tacgcctttg ttcaagtaaa ccctgagaaa ttagcggaaa acttgcaaaa gcaaggaagt
    17821 tatataccaa gcgtacgccc tggtaagggg actgaagagt atgtctcaag attactgatg
    17881 cgtttaagta cagttggtgc agtcttcctt ggtttagtgg cattgcttcc aatcattgct
    17941 caaatggttt gggatcttcc acaatcaatc ggattaggcg gtacaagctt actgatcgtt
    18001 atcggtgtag ctttagaaac tgcgaaacag ttagaaggct taatgctgaa acgtaaatat
    18061 gttggattca ttgagtaaga aagcgtgttg aggtttcccc tcaacacacg ttctcacaca
    18121 tttattagga gggaaaacaa tgaacctcat tttaatgggg ctgccaggcg caggtaaagg
    18181 aacacaagct gaaaaaatca ttgatgctta tggtattccg cacatctcaa caggagatat
    18241 gttccgcgca gcgatgcaaa acgaaacagc tcttggcctg gaagcaaagt cttatatgga
    18301 taaaggtgca ttagttcctg atgaagtaac aaatggaatc gtgaaagagc gactagctga
    18361 accagataca gagaaaggct tcttattaga tggctttcca cgtacgctag atcaagcaaa
    18421 agctctggac gcaatgctaa aagatttgaa taaaaaaatt gatgctgtca tcgatatcca
    18481 tgttggtgaa gaaatcttgg tcgaacgtct agccggccga tttatctgca gcaattgtgg
    18541 agcaacttac cataaaattt tcaaccctac aaaagttgaa gacacatgtg atcgttgcgg
    18601 tgggcatgaa ttctatcaaa gagaagatga taaacctgag acggttaaaa atcgtctggc
    18661 tatcaacatc aaaaatagtg aaccaatctt ggcttattat aaagaacaag gtttgctaaa
    18721 cactattgat ggtgctcgtg aaatcgatgc tgtatttgca gatgtacaaa aaatcattga
    18781 aaaatagcac gattgcccgt agccgacttg cattatgcag ttactcttgt caagttcaat
    18841 aaattatgat agaatatgtc agtccgactt ctagagaatc ctctagatga tccattttga
    18901 gaattttgag gtgggaactg cagtttccgc cattttatcg tgtgaaaatg cgaaacaagt
    18961 acaaggaggt actgtgcgtg gcaaaagaag atatgattga agtcgaaggt acagtcgtcg
    19021 aaactttgcc gaatgcaatg tttaaagttg aactagaaaa cggacaccaa gttcttgcta
    19081 ctgtttcagg taaaattcgt atgcattaca tccgtatctt acctggtgac aaagtaacgg
    19141 ttgaattgtc tccatacgat cttaaccgtg gccgtattac ttatcgcttt aaataattgt
    19201 actccgtaaa actacaggag gtattatcat gaaagtaaga ccatcagtaa aaccaatgtg
    19261 cgaacattgt aaagtaattc gtcgtaaagg acgcgttatg gtgatttgcc cagcaaatcc
    19321 aaaacataaa caacgtcaag gataattgga ggtgctataa atatggctcg tattgcagga
    19381 gtagatattc ctcgtgataa acgtgtagta gtttcactta cttatatcta tggtattggt
    19441 aacactactg ctaaaaagat cttagctgat gctggtgtat ctgaagatgt tcgcgttcgt
    19501 gaattaacga atgaacaaac agatgctatc cgtgcagaag tagataaatt aaaagttgaa
    19561 ggggatcttc gtcgtgaagt gaacttgaac atcaaacgtt tgatggaaat cggttcttac
    19621 cgtggaatcc gtcatcgccg tggattgcct gttcgtggac aaaacacgaa aaacaatgca
    19681 cgtactcgta aaggcccagc tcgcgctatc gctggcaaga aaaaataatt cttaagtgaa
    19741 ggaggttaaa acttcatggc agcaaaaaaa gtgaatcgta aacgccgtgt gaagaagaat
    19801 atagaagcag gtattgcaca tatccattct acattcaata acactatcat catgatcact
    19861 gatactcacg gaaatgcatt agcgtggtca tcagctggtt cattaggttt caaaggaagc
    19921 aaaaaatcaa ctccttttgc cgctcaaatg gcagcagaaa ctgctacaaa agcagcaatg
    19981 gaacacggat taaagactgt tgaagtaaca gttaaaggac ctggttctgg acgtgaagca
    20041 gcaattcgtt cattacaagc tacaggttta gaagtgactg caattcgtga cgtgactcca
    20101 gttcctcata atggtgcccg ccctccaaaa cgccgtcgtg tttaatgagt gccgctcatt
    20161 ttgagtttta tcagtaacgt cattgaacaa gacacttttc gttttgaaag gggtaaagat
    20221 aagaatgatt gagttcgaaa aaccaagaat cgcaaaaatt gatgaagaaa aagattatgg
    20281 caagttcatc gttgaacctc ttgaaagagg gtatgggact actttaggga attccctacg
    20341 tcgtatttta ttatcttctt tgccaggtgc tgccatcact aatattcaaa tcgatggtgt
    20401 actgcatgaa ttctctacca tcaaaggtgt tagagaagac gtcactcaaa tcatcttgaa
    20461 cattaaaggc ttagcgctga aaatgtatgg ccaagaagaa aaaacccttg aaatcgatat
    20521 taccggacct gctacagtaa ctgctggcga tattatcgtt gatagtgatg tagaaattct
    20581 taacaaagat atgtacatct gtagtgtctc tgaaggagct acgttccatg ctcgcttaac
    20641 agtgaaacct ggacgtggtt atgttcaagc agatgaaaat aaaaaagaag atatgccaat
    20701 tggcgttctt ccagtcgatt caatctacac acctgttcgt cgtgtgaact atcaagttga
    20761 gaacacacgt gttggacacc gtgaagattt tgataaattg acgatggaga tttggacaga
    20821 tggttcaatt gaaccgttgg aagcaatgag tttgtctgcg aaaatcatga ctgaacattt
    20881 ggatattttt gtgaacttaa ctgatgaagc aaaaaatgct gaaatcatgg tcgaaaaaga
    20941 agaaacacaa aaagaaaaaa tgttggaaat gacgatcgaa gagttagact tgtctgttcg
    21001 ctcttacaat tgtttgaaac gtgcaggaat caatactgta caagaactta caaataaatc
    21061 tgagcctgaa atgatcaaag tccgtaactt aggacgtaaa tcattagaag aagttaaagc
    21121 aaaattgcac gacttaggtt taggtttacg taaagaagat taaaaccttt tacgaaggag
    21181 ggaaaccaac gtgagttatc gtaaactagg acgcacatct agccaacgta aagcgatgtt
    21241 gcgtgattta acaactgatt tattaatcaa cgaacgtatt gtgactactg aagctcgtgc
    21301 gaaagaagtt cgctcaactg cagaaaaaat gatcacttta ggtaaacgcg gagatttgca
    21361 tgcacgtcgt caagctgcgg ctttcgtacg taatgaagtt gccagcgtgc gtgaagaaaa
    21421 tgaggacatc gtcattgaat cagctttaca aaaactgttc aacgatattg cacctcgtta
    21481 cgcagaacgt caaggcggat acacacgcat tttgaagaca gaaccaagac gcggtgatgg
    21541 tgcaccaatg gttgtcttag aacttgtcta aaatcacact aataacatca ctttatagat
    21601 gattctttta gagtgttatg atgttggaag atatttttcc gagtctagct cagcgctgct
    21661 cctcaagcga atatcgcttg gggagtaagc actcatcata aagtgttttt ttataccttc
    21721 aattacctgt catgacagat atgtttgaag gcataaaaaa acattttaag cgagaaataa
    21781 ttgaaaattt tgtctttttt tgatagtgtt agtaggaact ttaggatgaa gaaggagtaa
    21841 tcatggagcc aattattgaa ttagaaaaga taaattataa ataccagcca gatgatcttc
    21901 gccccgcatt gaaagatgtc tcctttacaa ttgataaagg tgagtggatc gctattattg
    21961 gacacaatgg ttcaggaaaa tcgacacttg ccaaaacgat caatggattg cttctgccag
    22021 aatcagggat cgtgaaagtg ggaaatcaaa tattggatga agaaaatatt tggacgatcc
    22081 gacagatggt tggaatggtt tttcaaaatc cagacaatca gtttgttggt tcgacagtag
    22141 aagatgacgt tgcttttggt cttgaaaatc aagggattcc cagagaagaa atgctagtgc
    22201 gtgtgaaaga tgcacttgag aaagttagaa tggctgagtt tgcttcacgc gagcctgctc
    22261 gtttatcagg agggcaaaaa caacgtgttg ccattgccgg cgtggttgcc ttacgacctg
    22321 atatcattat tttagatgaa gcaaccagta tgctagatcc agaaggtcgt gaagaagtga
    22381 tctctacgat caagaagatc aaagaagaaa gtcaattgac cgttatatct atcactcatg
    22441 atattgatga agcagctaat gccaatcgga tactagtgat gagacaaggc gaacttgtcc
    22501 gtgaaggtac accaaaagaa attttttctg ctggtcctga actgatcgac ttaggtctag
    22561 atctaccatt tcctgaaaaa ctgaagagcg cactgaaaga acgtggggtt gatgtgccca
    22621 gtgaatatat gacagaagaa aggatggtgg attggttatg gacatccgtt ttgaacaagt
    22681 agacttcact tatcagccga atacaccatt tgaacaaaga gccttatttg acatcaatat
    22741 gacgatcaaa gaaaacagtt atactgcatt agtagggcat accggaagcg ggaaatcaac
    22801 cttacttcag catttgaatg cgcttgttaa gccaacaagc ggtacagtcc atattggaga
    22861 acgagatatt cagccggata cggataataa gaatttaaag cccatccgaa aaaaagttgg
    22921 catcgtcttt cagtttccag aagcacagct attcgaagaa acggtagcaa aagacattgc
    22981 tttcggtcct aaaaactttg gtgtcagcga agaagaagca ctagtcctag caaaagaaac
    23041 attagaacaa gttgggctgg atgaaagcta tttggaacgt tcgccatttg aactttcagg
    23101 agggcaaatg cgtagagtag caatcgctgg tgtgcttgcc atgaggccgg aagtacttgt
    23161 attggatgaa ccaacggcag gacttgatcc ccagggtaga aaagaaatga tggagatgtt
    23221 ttggcgtctt cataaggaac agcagatcac tattgtatta gttacgcatt taatggatga
    23281 tgtagccaat tttgcagatt atgtctatgt gttggaaaag ggacgaatcg ttaatagcgg
    23341 ggagccgcaa gaggtctttc aaaacattga atggctaaaa gaaaaacaac taggcgtacc
    23401 tactgccacc gagtttgcag aagaattaat ggctaaaggg atgaactttg ctaccttgcc
    23461 tttgacagct gaagaattag cagacgctat cgtccattat gctggaggaa atgcccatga
    23521 tgaataaatt gatatttggt cgttatatgc ctggagattc cttgatccat cgattggatc
    23581 cccgagctaa attattagcc agtttttatt ttatcggaat cattttttta gcaaataatt
    23641 gggaaacgta ccttttgttg gcatttttca cattactttc tgtcttcttg tcaaaagtag
    23701 atttgaaatt ttttatccga ggcattcgcc cattgatctg gctaatcctc ttcactgtag
    23761 ctttgcaggt cttgtttaca agtggcggag aaacttattg gagttggggt gttttccata
    23821 tcactgaatt tggtgtgatg aacggcctct ttattttctg tcgattcgtg ttgatcattt
    23881 ttatgtcgac tctactcacg cttactacac cgcctttgga attatcggat gcgattgagt
    23941 acttgcttcg tccattgaaa gccgtacggt tcccagtcca cgaaatttca ttgatgctgt
    24001 cgattgctct tcgttttgtg cctacattga tggacgaaac agaaaagatc atgaacgctc
    24061 agcgcgctcg cggtgttgat tttggtgaag gaaacctagt tcagaaaatg aaagcagtca
    24121 ttcctttgct gattccttta tttgtcagca gcttcaaccg tgcggaagat cttgcgactg
    24181 ctatggaagc aagaggctac aaaggtgggg aaggaagaac gaaatatcgt gtattgcatt
    24241 ggcataatag agacacgatg gttatcattg cttttgtatt attaactgtg ctactgatct
    24301 ttttgagagg ataaacgttc agtgggccgc tatctattct tttcttgctg atctgtttgc
    24361 aagaaaggat gcgagcagtg tatgacaaga aatattaggg tctgtggcag actaaggtca
    24421 caggcctctt tcttttatct gaaaagaaag aacgaaaaaa ttttataaaa aaatcattat
    24481 tccagataac cttttaggaa caaaggagag aaaaacatgg tacgttacaa agcaatcatt
    24541 gcatacgacg gaacaaattt taatggattc caaaaacagc caaatgggcg tactgttcaa
    24601 gaagaagtag aaaaaacatt acaaaaaatg gcaaatggaa aagaaatcac ggtctttggt
    24661 tcgggaagga cagatgccgg agtacatgcg attgggcaag taatccattt tgattatcca
    24721 gaggagcgtc cgttagaacg aatgcgtttc gcgctagata cccagtcacc agaggatatt
    24781 gctgtaagac aagtagaaat tgtttcagaa gacttccacg caagatattt ggtcaaagaa
    24841 aagacgtatc aatttcgagt ggatataggg aaaccacgca gtccttttcg acgccattat
    24901 gcaagttatt ttccttaccc tcttgatttg gaaaaaattc agcgggcgct tcctgattta
    24961 ttagggactc acgatttcac ctctttttgt gcatcaggaa gctcgataga ggataaagta
    25021 cgtacgatct acgaggctaa gatggaggta aatgaaacaa aagacgaatt gttgtttaca
    25081 tttaggggaa atggcttctt gtataagatg atacggatct tagtaggaac gcttttgaaa
    25141 attggcaatg gccgattgcc tgaagatagt atcccagata ttattgcgaa aaaagatcgt
    25201 aatgcagcag gacctacagc acatcctgaa ggactttatt tgtatgaagt gaaatatgaa
    25261 taagaggttg tgacagaagt ggtcagcttc aagaaataag aaggaattta cgaaaattac
    25321 ttctcaaaat tttgcaaaat ttcggcttat tttcgaagga gctacttctg gaacaccgtg
    25381 aatcagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacaacca aacaaaaata
    25441 gctcgtcata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    25501 tttaaggaag aggttgtgaa atcagcgttt tgacctgagt attagagaaa caaacggaaa
    25561 aatagttcct catattttgc cgtttgttga gctaataccg aaggtcagct gattgaacac
    25621 cgtttaagaa agaggtcgtg agcctgtcat ttaactctga gtcacaagta acaatttcaa
    25681 aaaacagctt ctcatctttg atgaatatcg tgacttgtgg acgaggcgtt gataagtgaa
    25741 tcatcttttt gttacctatt ttttctgtaa ccaaaaaacg atggaacgtt gttttgattg
    25801 cccttaatga ggacagtcgg aacaggtttg tcatttttat atttaaatgg cacaaatttt
    25861 tctaattatt tgtcatttag cgctgaaaat ttttgctatt tatctaaaaa agtgataact
    25921 acttaagatg aagcagaata tttacgccat acggcataca accgcgaaaa atataaaaaa
    25981 aagaaaacga ttgaacgcgt ttttgcagtc gcaaaggaaa agcatggaat gcgctacatc
    26041 aagtatcgag acttggaaaa caatcaggtg tatacgctga ttgtttttgc tgcaatgaat
    26101 ctgaaaaagt tgtccacttg gcagttggaa catatgtctt catatctttt attatacaaa
    26161 aatagcaaaa gcaccagtct acactcttag taaagaggaa actgatcctt ttgtctacat
    26221 tcttaaagta cttaaact
//
LOCUS       DAYYHX010000017        25264 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000017 DAYYHX010000000
VERSION     DAYYHX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.017"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(119..565)
                     /gene="dtd"
                     /locus_tag="ACHA6Y_000525"
     CDS             complement(119..565)
                     /gene="dtd"
                     /locus_tag="ACHA6Y_000525"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM8986642.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(576..2789)
                     /locus_tag="ACHA6Y_000526"
     CDS             complement(576..2789)
                     /locus_tag="ACHA6Y_000526"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM8986643.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(3022..3786)
                     /locus_tag="ACHA6Y_000527"
     CDS             complement(3022..3786)
                     /locus_tag="ACHA6Y_000527"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM8986644.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(3787..4734)
                     /gene="prmA"
                     /locus_tag="ACHA6Y_000528"
     CDS             complement(3787..4734)
                     /gene="prmA"
                     /locus_tag="ACHA6Y_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM8986645.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(4747..5235)
                     /locus_tag="ACHA6Y_000529"
     CDS             complement(4747..5235)
                     /locus_tag="ACHA6Y_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM8986646.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(5201..5872)
                     /locus_tag="ACHA6Y_000530"
     CDS             complement(5201..5872)
                     /locus_tag="ACHA6Y_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM8986647.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            6010..7296
                     /locus_tag="ACHA6Y_000531"
     CDS             6010..7296
                     /locus_tag="ACHA6Y_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM8986648.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            7368..7562
                     /gene="ssrS"
                     /locus_tag="ACHA6Y_000532"
     ncRNA           7368..7562
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHA6Y_000532"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7640..7924
                     /locus_tag="ACHA6Y_000533"
     CDS             7640..7924
                     /locus_tag="ACHA6Y_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986649.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            7997..8476
                     /locus_tag="ACHA6Y_000534"
     CDS             7997..8476
                     /locus_tag="ACHA6Y_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8986650.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            8699..8842
                     /locus_tag="ACHA6Y_000535"
     CDS             8699..8842
                     /locus_tag="ACHA6Y_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM8986651.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            9187..9315
                     /locus_tag="ACHA6Y_000536"
     CDS             9187..9315
                     /locus_tag="ACHA6Y_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986652.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            9420..11003
                     /gene="abc-f"
                     /locus_tag="ACHA6Y_000537"
     CDS             9420..11003
                     /gene="abc-f"
                     /locus_tag="ACHA6Y_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM8986653.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(11440..12624)
                     /locus_tag="ACHA6Y_000538"
     CDS             complement(11440..12624)
                     /locus_tag="ACHA6Y_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM8986654.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(12649..13656)
                     /locus_tag="ACHA6Y_000539"
     CDS             complement(12649..13656)
                     /locus_tag="ACHA6Y_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8986655.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(13828..14187)
                     /gene="comGG"
                     /locus_tag="ACHA6Y_000540"
     CDS             complement(13828..14187)
                     /gene="comGG"
                     /locus_tag="ACHA6Y_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM8986656.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(14159..14584)
                     /gene="comGF"
                     /locus_tag="ACHA6Y_000541"
     CDS             complement(14159..14584)
                     /gene="comGF"
                     /locus_tag="ACHA6Y_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM8986657.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(14547..14894)
                     /locus_tag="ACHA6Y_000542"
     CDS             complement(14547..14894)
                     /locus_tag="ACHA6Y_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986658.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(14866..15336)
                     /gene="comGD"
                     /locus_tag="ACHA6Y_000543"
     CDS             complement(14866..15336)
                     /gene="comGD"
                     /locus_tag="ACHA6Y_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM8986659.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(15311..15589)
                     /gene="comGC"
                     /locus_tag="ACHA6Y_000544"
     CDS             complement(15311..15589)
                     /gene="comGC"
                     /locus_tag="ACHA6Y_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM8986660.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(15586..16635)
                     /gene="comGB"
                     /locus_tag="ACHA6Y_000545"
     CDS             complement(15586..16635)
                     /gene="comGB"
                     /locus_tag="ACHA6Y_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM8986661.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(16592..17521)
                     /gene="comGA"
                     /locus_tag="ACHA6Y_000546"
     CDS             complement(16592..17521)
                     /gene="comGA"
                     /locus_tag="ACHA6Y_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM8986662.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            17653..19407
                     /gene="ade"
                     /locus_tag="ACHA6Y_000547"
     CDS             17653..19407
                     /gene="ade"
                     /locus_tag="ACHA6Y_000547"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM8986663.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(19505..20167)
                     /gene="trmB"
                     /locus_tag="ACHA6Y_000548"
     CDS             complement(19505..20167)
                     /gene="trmB"
                     /locus_tag="ACHA6Y_000548"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM8986664.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(20237..21019)
                     /locus_tag="ACHA6Y_000549"
     CDS             complement(20237..21019)
                     /locus_tag="ACHA6Y_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM8986665.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(21223..22434)
                     /locus_tag="ACHA6Y_000550"
     CDS             complement(21223..22434)
                     /locus_tag="ACHA6Y_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8986666.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(22427..23164)
                     /locus_tag="ACHA6Y_000551"
     CDS             complement(22427..23164)
                     /locus_tag="ACHA6Y_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8986667.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            23302..23736
                     /locus_tag="ACHA6Y_000552"
     CDS             23302..23736
                     /locus_tag="ACHA6Y_000552"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM8986668.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            23729..24073
                     /locus_tag="ACHA6Y_000553"
     CDS             23729..24073
                     /locus_tag="ACHA6Y_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8986669.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            24253..25263
                     /locus_tag="ACHA6Y_000554"
     CDS             24253..25263
                     /locus_tag="ACHA6Y_000554"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8986670.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         7642 a         5264 c         4504 g         7854 t
ORIGIN      
        1 ttccgactgc tgacaaaaga agcgcttttt gcagacaaaa aagccactta agttagagtt
       61 tatcagtcta acttaagcga cgtgtttcat agtttttact agtgaaaacc agattacttt
      121 atttgttttt agaatcaata atgatcgtta caggtccatc attgacgaga gaaaccgaca
      181 tatctgctcc gaattgtccg gtagcgactg tgatgccgcg acttctgatt ccttcattga
      241 atgattcgta taaaggaata gctgtttctg gtcgtgcggc ttccacaaag cttggtcgat
      301 ttcctttttt cgtatccgca aacagtgtga attgagagat acttaaaatt tgtcctccca
      361 catcttctac actaatattc atttttcctt gttcatcttc aaaaatacgc atttgtgcaa
      421 ttttttttac cagatagtcc acatcttttt gcgtatcggt ttcatgaatg cctaataaaa
      481 caagtaggcc gtagccaatt tttccaattt cttgctgatc gactgacaca gcagctttac
      541 ttacccgttg aacaactgct ctcatatctg tcctcctatc cattcgtccg gcgtacactg
      601 tagacatcag gtactgcttt gattttatcc acgatactct ttaggtggga taaattctgg
      661 ataccaaccg tcaaatggat cgtcgccatt ttatctttat tcgtccgtgc ttcaacgctg
      721 ttgagatttt tcgttagcgc attgactgtt tgaagtacat cattcaataa gcctgaacga
      781 ttatagccgt aaatttccaa atctgcatca tactcttttc gtgtattcga tgtatcttcc
      841 cattcgactt caatcaaacg ttctgctaca tttggtttgt caggctgaac attcggacaa
      901 tctcgacgat gaatggatat ccctctgcct ttagtgatat aaccaacgat atcatcacca
      961 ggaatcggat tacagcagcg actgatacga attagtaagt tttctacccc ttgaatgaca
     1021 acgccacctt catgacgtac tttcattttg tcaggttctt ttttcggctg attcatgatt
     1081 tcttctgctt cttgcttttg ctgctcgatt ttttgttctt tacgttcttt ttcagtcaga
     1141 cgattggcca tcgtcaaggg gctaacttct ccatacccta cagctgcata gagatcatct
     1201 tctgtttgat aattaaaacg atcgagtgct tcttgcagtt tgttcttcgt caaaatatct
     1261 ttaggcgtaa atcctagatc tgtaatacat ttgaccacgg attcgtggcc tttgatcaca
     1321 ttttcttctc gatcttgggc tttgaagaaa cgtttgatct tatttcttgc tttgctagta
     1381 gcaactaatt tcaaccagtc gcgacttggg ccaaatgaat ttggagaagt catgatctca
     1441 atgatatctc cgtttttcaa tttgtaatca agctgcacca ttttgccatt tacttttgca
     1501 ccagtggttt tgttaccgat atctgtatgg atactgtatg caaagtccaa tggtccggat
     1561 ccttttggta actcagtaac gtctcctttt ggtgtgaaga cgtagacttt atcactaaag
     1621 atatctcctt ttacgccttc cataaattca gaagcatcat agctttcgtc ttgaagttcg
     1681 agtatctcat ggaaccagct taattgtttc gtcataccat ctggttctac tttttcattt
     1741 tttccttctt tataggccca gtgtgcagca accccgaatt cagcgatttc atgcatttcc
     1801 tgtgttcgga tttgaatttc taccggattt cctgccggac cgatcacggt agtatgcaaa
     1861 gattggtaca tattagcttt tggcatggca atataatcct taaatctgcc gggcattggt
     1921 ttccattttg tgtggattgc tcctaataca gcataacaat ccttgattga atctacgatc
     1981 actcgaatag ctagcaagtc ataaatttcg ttgaactgct ttttctgatc tttcatttta
     2041 cgatagattg aataaatgtg ttttggccgt ccatagattt ctgcaaaaat ccccagctct
     2101 tctgtcgcta ttcgaatatc ttcaacagta ccacttacgt atttttcacg ttcttctctc
     2161 ttcgtctgca tcaagtggac gatacggtaa tactgttttg gatttagata acgaagtgct
     2221 gtatcttcta attcccattt gatccggctg atccctagac gatgtgcaag aggcgcatag
     2281 atttctaaag tttcctgagc gatccttctt tgcttatctt ctcgcaggtg cttcaacgta
     2341 cgcatgttat gtagtctatc agctaatttg accatgataa ctcgtaaatc ttgagccatt
     2401 gctagcagca tctttcggtg gttttctgct agctgctctt cgtgggattt atattttatt
     2461 ttccctaatt tggttacgcc gtccactaac atagcaacgt catcgccaaa ttcttctctc
     2521 agatcttcta aagtgatttc tgtatcttca acaacatcat gtaagaagcc tgtagccact
     2581 gtatggggat ccatttttaa atctgctaat atacctgcga cttggatagg atgaataatg
     2641 tagggttcgc ctgattttct aaactgtccg tcatgtgcag cagtcgcgta ttcgcatgct
     2701 ttctcaacaa atgctacatg ttcgggaccc atgtattgac taacgagctt tatgacaccg
     2761 ggtcccgtta aaatttcttc ttttgtcaca ggaatcgatc cccctttctt ttttcggtaa
     2821 aacgggaatc gggacaaata tctgtttgat aattgtccca aaatccgtgc agttatacgt
     2881 ttttttacgg aaatgttcgc tttttaaaag ctttttcaaa cgatttttcg tatccattgt
     2941 ataaataatt gtttctatta tacaaattgt tgactatttt ttcaattctt tctgtcgata
     3001 ctcttttatt aatgaagaac gttagctgaa agaaggattc attaattccc attgatagct
     3061 agcagcactt aataaataaa gaggtgctgt ttctgtacga aggattcttg gtcctaatcc
     3121 acaaagtatc cctttgtgtt ctacaaaata atcgacttca tcgcgggata atccaccttc
     3181 tggtccaaag acagctaaga ttcgactatc tggttttgtt tcactaagaa ttttggctgc
     3241 atttgatttt tcgccttgct ttgctgattc ctcataagca accaagacca cgtcataatc
     3301 atctagccgc tcaaataaat cttgtttcgt ttccaataac tctactgatg gcacgatctg
     3361 ccgatgggat tgttccgctg cctcttgagc aatcttttca agccgttgtt gtcgtttcat
     3421 acgttttttt gcatcccatt taacgacaga ggctgatgcc gggaaaccaa gaaaagcttg
     3481 cgctcctaat tcagtggatt tctgaacgat ccaatccagc ttttcccctt taggatagcc
     3541 actcgctatg gtaatcgaac ttggcagttc cttttgattt tcttcttttt taatttcttt
     3601 cagataaaca gcctcttcag taatctccgt gatttctgca acaatggcta tttgatcatt
     3661 gaatgccaaa aaaacttcat cctcgggttc catcctcata acccgtacaa tgtggtgata
     3721 gttttcccct tctaaaacaa aatagtcttt tgattcatat gattctttta aaaaataccg
     3781 ctgcatctat tccacctctg gttttttcaa aataattgca taccagtctt tttgctggaa
     3841 aatttgatcc acgataaacc cttgttctaa catggcatcc agaaccattt gcttcttctc
     3901 ttcaatgatt cctgaaacga tcagcgtgcc tgtttgcttc agtaaacgcc aagcatctgg
     3961 gatcatcaat aagatgatat ctgctaaaat gttcgcaacg attacatcac tttcaatctc
     4021 gattcctgtc aaaagatcgt tagctgatac atggacatct ttggctattt cattcatatc
     4081 catattttct ttcgccgaac gaacggctac ttcgtctaaa tcaaaggcat agacatcttt
     4141 tgcaccaaga tattttgctg caatacttag cacgcccgac ccagttccca catctagcaa
     4201 tgtttcacca ccacgcaaga ctgtttctag tgctgtcaat gtcaaggaag tcgtgggatg
     4261 agtgcctgtg ccaaatgcca taccaggatc tagtgtgata attttttcta gaggatcttc
     4321 tgtctggtag ttttcccagc ttggcgcaac cgtcagcatt cgcgtaatac gcaccggatg
     4381 atagtatttc ttccaggctg ttgcccagtt gctttcctct acttcactaa ctgttacttt
     4441 attttttcca atagctagac caaactcagg taattgtgtg atacgatctt tgataaaagg
     4501 caagatttcc ggtaagaaag tcgtttccgg aaaataagcc atgactagtg cgccttcttt
     4561 cagcgatgta aaatcatttt tatctaaaat ctcaccatat tgatccgact gaaaatgttc
     4621 aacatctaaa gaatcttcaa tcgcgacacc actcgcccct gcctccatca aaatattagc
     4681 aactgcttcg actgcttcac tttcagtttc tacttttact tcattccatt tcatatctgt
     4741 cactccttaa taacttgggt acggtaaata agtatctgct tcattttgct gttttacttc
     4801 tactaattct gctagttgct gattatccca gtgaagctcg aatacagctt cgtcatcgtc
     4861 ttcatccaaa aaatcaagca aatcgctttg cccttgagct aatacttcat ttagatattc
     4921 cactaaagaa tcagctactg attttgccaa cccttttttt ccttcataag ggattactgc
     4981 tagataattg cgttcatcaa aaacagattt ctgaggatta aaaagaagta cgccgtcttc
     5041 aaattcgata attggttctt gcgaaacgat cccttctgca tcatcaatct caaattgtgc
     5101 tttattttct gcaaacaagc gaacaacgat ctcgatcgta tgattttttg tatcccaatc
     5161 taaagctaca tcatagtctg tcacttgttt ttcaattatt tcatctaaat atgttaatag
     5221 atccttcttt ttcattttct ttcctccaac cccaatcttt ctcgatttgg ttttttcttt
     5281 gtaaagagat ataaggattt cccgacacaa caaaacgcaa cggcttttct gtccatacac
     5341 ctttgtttgg gataccgatt ctaggcaaag cattgatctt ttttggtgtc ttttttttct
     5401 caaaaactaa gcgtaacgaa ctatctgcta tagactgacc ataaagttct tgaggaattg
     5461 ctaatgcttc cactaatttt cccggaccgt tgctgatatc tggaccagtt tttccgccac
     5521 gatttttact catttgatca atcattgcgg cgggttcgat tgcacgtatc attactcctt
     5581 gaggaatccc ttctggctga gtaatgatgt tcaaaatccg atgagtatgc atcgtgtaaa
     5641 ggtagatcgt acctggtttt tcatacattg cccgcacacg cggtgttctt cttaaaccat
     5701 agctatgggc ggcctcgtct tctggaccta ggtatgcttc cgcgtctaca atatatccag
     5761 ctaatcttcc ttctggtgtg tcatgctcca aatacatccc caataaatcc cgtgccactt
     5821 ctggcgtcgt tttattccaa aaaaattcat ctatcttttg tttatccgtc atttttctca
     5881 cctactttat catacacaat attagttgga taagctatga aaacaaaaca acagaaggct
     5941 gttctttcta ggcatcttgc taaatatgat ataattaagg gacgtaaaaa caagaaggag
     6001 gaaaatccta tgcaacagcc attggcttat cgcatgagac ctcgaacgat cgatgaagtc
     6061 gttggtcagc agcatctagt cggggaagga aaaatcatac gccgcatggt cgatgctcgc
     6121 atgctctctt ccatgattct atatggacct cctggtactg gaaaaacgag tatcgcaagt
     6181 gcgatcgccg gctctactaa ttatgcattt cgcatgctaa acgccgctac agattcaaaa
     6241 aaggatctac aagttgtagc ggaagaagca aaaatgagtg gtacagtgat cctcttattg
     6301 gatgaagtac accgattaga taaaacaaaa caggattttc tgcttcctca tttagaaagt
     6361 ggccgaatca tcttgatcgg agcgactact gagaatcctt atattaccat caatccagcg
     6421 atccgcagcc gtacacagat ctttgaagtc aaaccattaa cagaacaaga catccagttg
     6481 gcagtcgaac atgctttaag ggataaagaa cgcggccttg gacaacaagc aatccagtta
     6541 gacgaagatg ctcttcttca cctttcgcgt gcaacgaacg gcgatttgcg gagtgcattg
     6601 aatgggctgg aactggctac actatcgact ccttcagata aagaaggaag gattcatctt
     6661 acactgtcga tcattgaaga atgtgtccaa agaaaagctc ttacccatga caaaaatgga
     6721 gatgctcatt atgacgttat ttcggctttt caaaaatcga ttcgtggaag tgatgtggat
     6781 gctgcacttc attatctcgc ccgtttagtt gaggcaggtg atctggcaag tatttgccgg
     6841 cgtctgatgg ttatcggtta tgaagatata ggtttaggta atcctgctgc tgcggctcgt
     6901 acagtgaacg ctgtattagc tgctgaaaaa ttgggattgc ctgaggcacg tatcccacta
     6961 gcagacgtcg tcgtcgatct ttgcttgtcg ccaaaatcca attctgccta catggcatta
     7021 gatgcagcac ttgctgatat tcgagagggc aaagctggag atgttcctga tcatttgaga
     7081 gatagccatt acaaaggtgc gaaatcactc aaccgtggcg tgggttatca gtatcctcat
     7141 cattttgatc aggcttgggt caatcagcaa tatttaccag ataaactgaa gaatgctcag
     7201 tattatcagc caaaagatac agggaaatat gaacaggctc tgggtcaaca gtattaccgt
     7261 ataaaagagt ggaaaaagca tcctccaaaa aactagacaa gaaaacggtt ctgtttctgc
     7321 gagctctctc attgctcttt ttaaattcat atgctactat agaggtggga aatctgtgat
     7381 gttcgaattg tcctctttgt gtgttgaccg aacattttta ttgatatctt gggacccgtc
     7441 ttgtgtcact gctggtaaca tgccttatca tttggtagtt cgaagctacg accatgggac
     7501 ccacctgctt gaattcgcgg gatcaatact acaggacaaa caacggcatt aacggagatt
     7561 accatctttt aaagaataaa gcaatttgct cttggcaaat tgctttttct atttgttgct
     7621 tattttggag gatttgttca tgattaggat tttaatgatt atcgccactt tagtattatt
     7681 attcgttagc tattatttat ttaataaaca ggatatcttt tttgtattga tcaacaaaaa
     7741 tgataaaaat caaggattcc tacaatttta cggagctgca tatgctgttt taggcgtaat
     7801 gggtatttta acagcttttt tcaatcaacg ctttattgcc ttggtctttt tactgatcgt
     7861 aattattgtt tctgccacct tcagtatccg atttgcaaaa aaaattgctg aaccaaagca
     7921 gtaaataatt ctctttttcc ctaaagtgtt ctacaataaa agagtacaat acgattgact
     7981 tgaggagtga atgcttatgt tacaacaata caaaaaaatc atggttgccg tagatggttc
     8041 tgatgaagct gaattagctt ttaaaaaggc ggtcaatgta gctatccgaa ataacggaga
     8101 acttctatta gcacatgtca ttgacacacg ttctttccaa accgtttcat cctttgatgg
     8161 aatgcttgca gaacaagcta ctgaaatggc aaagcaaaca ttggcagatt atgaaagcaa
     8221 tgcgaagaaa gctggcttga ataatgtaac ctctgtcgta gaatatggct caccaaaaca
     8281 aattattgcc agagaaatcc cagaagataa tcaagtagat ttgattatgt taggagccac
     8341 aggattgaat gctgtagaac gtttatttat tggttctgtt tctgaatatg ttattagaaa
     8401 cgcagcttgc gatgtgcttg ttgtccgaac tgatttagaa aaccaattgc cagctaaaga
     8461 ccacaccaaa gcttaacacg tagacgacct gccaactatc ggcaggtttt tttgcacaaa
     8521 aaaccgtgat aaaaacaaaa gacgcggcaa gcactcatcg gtctttataa tgtttaaaat
     8581 aaagggaaaa cagtcaatga gttcaagtaa aataaaaaaa agtgaacacc agggcgatta
     8641 gtcgccctgg tgtggtttaa aacgacctaa gcatgccaaa gcttattggt tcgtcaatgt
     8701 ggaagtttcc gatgaatctg atacgaaaaa atatgaagta tcgatggcgg ataccgattt
     8761 tatcgccaaa ctaaaatttt atttcgggtt cgggtaccgc gagaaggcaa gtgctacatt
     8821 cggtaaatca ttcaagaatt gatgaaaggc taccgcttgc cattgcaacc acaacgatac
     8881 tcctgaacgc gatgttggtg agatttccaa agagaagagc tgtttaatgc aacggtatta
     8941 agcaaatggc aaaaggaagt catgtagaca aagtttttta tcaaacacca tatgatttcg
     9001 cctgaacgct ggtttagctc agaatacaat gatgtggtcc acgagacatt accctcagtc
     9061 agataaaaaa agctctctga cagaattaca catcgataaa tattaagtaa atgtcagcgc
     9121 tagttgaact ttctgacact ttcaggtaca ctggaaaaga caactacacg aaggagggaa
     9181 aatactatga tgcgcatgac taaaataaca agtaatgaaa attaccaacg ataccatatc
     9241 gcatttagcc atgcaattgg ttttacagtt gctgttgacg ggacaagttt gtccaaaaca
     9301 gttgaattca cgtaaaatca atggccatct caaggtattt ttctcatgat aatctgagtt
     9361 taaataacac aggccatgga taaccatggc ctttttgagt ggtctcaagg agagagatca
     9421 tgatcataca agcaatttct attaaaaaaa caatcggcgg gaatccatta tttgaggatt
     9481 tatcctgtca aattgaagct ggtgaaaaaa ttgtcttagt cggtatcaac ggaactggca
     9541 aatcaacctt tttacaaatc ttgaccggca gagaaggtgt cgacagcggt gttatcagtc
     9601 gcaaaaaagg cttaaagatc gggacggtcg aacaagaact tacagtttcc gaagcgaccg
     9661 tcaaccacta cttgctccat tctgccgctg agatacagga cctcaaacag caaatgagtc
     9721 gctacgaagc attgatgaca gaaccagata caaacttaga gaagtgtctg gcccgttacg
     9781 gtgagctgca gcatcgcttt gaagaactag gcggttacgt attagaagac cggattgcag
     9841 cgatcttgca aggcttaggg attccacacc gtcaggaagc acggttaaca gaacttagcg
     9901 gaggcgaacg agttaaagtc gctttagcca aaattttagc agccgatgcc gatttacttt
     9961 tactcgatga accgaccaat catttagatc ttaacagtat tcgctggttg gaaaactatt
    10021 tacagaatac aaaaaaatcg gttctggtcg tttctcatga tcggcaattt ctcgatcaag
    10081 tcaccacaaa aacatgggaa ctggaagaag gtgcattgat tgagtatccc ggaaactaca
    10141 gccgttttcg agtcttgaaa gaagctcgct tggctgagct gacaaaaaat tacgaactgc
    10201 agcaaaaaga agtccagcgg ttgaaagtca tgattcgtcg ttttcgtcag tgggcacatg
    10261 aaggagacaa tgagtccttc ttcaaaaaag ccaaagaact ggaacgccgc ctggcgaaac
    10321 tgacactcgt caaaccgcca cctcccccga aaaatcggct gcaaagcttg tctaatggtg
    10381 gaaaatctgg caaagaagtt ttcatcattc aaaacttgca ccagcagtac gctgatcaag
    10441 tactattcaa agacagcagt tttgcagtct atcgtggaga tcatttagcc atcatcggcg
    10501 acaatggtgc aggaaaatca accttactca aattattact cggtgaggaa aagccttacg
    10561 gtggcaccat caaattaggt agtagcttgc aaatcggtta tttgccgcag cagctgcagt
    10621 ttgccgatcc cgatagccgt ttgctggctt acgccatcac attgactgga aacgaagaag
    10681 tcgctcgacg gcaattagcc aaaagtggct tttatcaaag cgatgtgggt aaacggatca
    10741 aagatttatc cggtggtgaa aaaatccgtt tggcgttgct gaaactcttt ttagaaaaaa
    10801 tcaacgtcct gattttggat gaaccaacca atcacctcga cagctatgct cgagaagaaa
    10861 ttgaagaaat gctgcaagac tatcaaggaa cgctcctagc agtcagccat gaccgctact
    10921 ttttgcagca acacttccaa gaagcactcg ttattgatca ggagcagatc agccgccagc
    10981 cgctcggcca actgattgac taggacgttt tttcgcaaca aaaaacttga ttttcatacc
    11041 tgtgctttca cgattcaggc aaagtacagg tggaaaatca agtttttatt attcgatcaa
    11101 tgaatgtttc ggatcggttt tctagctaaa cataaagcga agaataaagt cgacagccca
    11161 cgaggctgat ctaaaaatgt tttttctttt tacatatgta atgtccctta taaagatgaa
    11221 tgaaaataaa atattgggat tttcctgaaa atcaataaat aaaatgccag tcttttccct
    11281 tttgaccact tttccaagca aaattcccta agaagatcct gtaaaaaaga agggaaattg
    11341 aagttaaatt accattttgc ggtcttctcc gcaaaaaaaa cacatgcaag aattccctct
    11401 tgcatgcgtt tcatctttta tcctaacgaa tgctttggct tagccgcgca gacgctccac
    11461 atcacgggca atcattagtt cctcatctgt tgggatcact aatactctta cttttgattc
    11521 ttttgtagag atatctactt cttcaccacg caattcattc aacgctggat cgatttcaca
    11581 accaaaccaa gacatacctt tgatgatttc actacgtaca tgtccgtcat tctcgccgat
    11641 accagcagta aagacaattg catcaacacc attcatcgtt gtcacataac cgccgatgta
    11701 ttttcggatg cggtccacga aaatgtcata agctaaacga atatgttctt tttcatagtt
    11761 cttttcaaga tcacgcatat cactagacaa tcctgttagg cctagcagac cagatttctt
    11821 gttcaaaaca ttgatcattt catcaatatc aatctctaat ttttccatta aatatgggag
    11881 aacagctgga tcgatatcac ctgaacgtgt acccattgtc accccagcta atggtgtgaa
    11941 tcccatagaa gtatccatgg atttacctcc atcgactgct gtgatcgata caccatttcc
    12001 taagtggcaa gtgatgattt ttagttcttc gatcggtttg ttcaataatt ttgccgcacg
    12061 ttccgacaca taacgatgac ttgtgccatg gaagccatat tttcttactt tgaaatcttc
    12121 ataataagac accggtaagc tatataaata attttgcttt ggcattgttg agtggaaaga
    12181 tgtatcgaat acagccacac tcaaaatgtc aggtaaaatc tttttaaacg ctttgatccc
    12241 cattaagttt gcaggattat gcaaaggagc aaattctgct aattcagcaa tgcggtccat
    12301 tactttctca tcgatcacta ctgaatcccc ataagtttct cctccgtgaa caacacggtg
    12361 ccctactccg gtgatttcat cataagagct taaaatttgc agttcaatca actgatctaa
    12421 cagcattttt actgctactt cgtggttttc gatatccatg atttgttcga atttttgatt
    12481 atcgccgtac ttgatggtaa aaatagaatc ttgtaaaccg atacgttcga cgatcccttt
    12541 cgcaactacg gtttcttctg gcatttgata taattgccat ttcaaacttg aactcccagc
    12601 attgattgca attgttttag acatatgact ctccttctcg ttgaatgatt acagagcaga
    12661 tgatttccag tttttaaact cgttgaaaaa ttccgtcacc ttctctggtt cttttaatga
    12721 agcaagtttc acaagcaaga cttcttttac ttgttgtgct tgtggtcctt ttttctgtaa
    12781 gatcaaaata cttttttgcg attgtttatt acggaacaat tcatctggca attgaatcat
    12841 tccttgaaga taaccttctt ctttaaacca ttttttgatt tcttcacttt gatccgtttc
    12901 taaaaatcca ctcggcatca aaaacaaacc aaaaccatcc ggcttcacat atttcattga
    12961 ttgctctagc aataaatgat gtgcataact atggccttct tcggtacttg ttaaaaactc
    13021 ttttgctttt tcgtcattcg gataataacc gattggcaaa tcgctgattg caaaatcgac
    13081 aggatctatt aataattcct gcaacccgtc ttgatgaaag tattgaacat ttgcttgtgt
    13141 caaatcactt gtagaagctg cgacagccaa caatgtatca tcaatatcca caccaattcc
    13201 ttctgtttgg taccccgcat tcgataggtt taaaagaact gttaataaca agttgcccat
    13261 tcctgccgca atatccagaa tcttaaccgg tgctttttta tttgtgtata gttgttcaat
    13321 aagaaaaaca aataaaaaac cgatactatc aggagtgagt tgatggttcg cctgcaaatg
    13381 ttctgtctgg ctgcccttca ataaaagtaa ttgagacagt ctgcgccatt cttctggctc
    13441 gaaagaaagt ttcttcaact cttcataaag agctgtgatc cgttgcgttg tctcttttgt
    13501 cggcactcca tccaccacac gtacttgata atcatctatt aaattttctg cattttcgac
    13561 ataggcttct aaaaaggaag ttcccaatgc attctgtaaa agctgaatag cttccaggtt
    13621 ttgattaaac ccttgttcca ttttttctgg taacatggtg cacctcattt ctagatatat
    13681 tataacaatt ttttcacctg tttaattcta tcgaacaatt gacaaaattt caatcgtttt
    13741 cactgttttt tatttgtgaa acggcaaaca tttttttatt aaaaacagtg ttataacaga
    13801 aaagagggcg ttttcacgaa cattccatca ttctgtacta gtatctgtat cattttttac
    13861 ttcttctgtg aaccgaaaga taaacggtga gacttccacc tctaagaata gggactgttg
    13921 tacccactga tatgttacag atccagttgt gtagtaaaaa actccttccg aactttcggc
    13981 aagcgtagac tgttccgata aagccatctg cttcattatt tttgctatat aataattttt
    14041 gattcgtata ttaagatcca ttgacaaacg gtagctttct aatgctgcca tcaataaaaa
    14101 cgtacataaa agaaataaaa gcaagacaga aaataaaata gagcccttat aacattttct
    14161 actgatcgtt ttttgtccat atggcatatc catcttctcc attttcaaag tgtacataaa
    14221 aagaaattgt gtgttctttt tccaataatt gaagctcagt gatacttgtt aacataggtt
    14281 gatgtccgcc ggaagttctt cttttacgta tcattgcctg atatctttcg atgatatact
    14341 gtttatcctc gatcgtgtag tgcagttgct gatttttcac agagacattg tgactgctct
    14401 tgagttcttc ttctaattgt gctaaaaata ttagccactc tttttgatct ttacgctgca
    14461 aaaattcact tacttggatg gtttgctgaa tcaaaagatt gaatgtgagc aatagactgc
    14521 tcaatatcat cagtccaatt agacattcaa tcagcgtaaa tgctggaatt acttgttttt
    14581 tcaatactga tcgcttcttt ctccatattg acaaacactt tttcgattac tccattatta
    14641 tcgacaattt ggatatcgta tgggatcatt tcttcccttc gctctggtct tttgtgatac
    14701 atccgctgcg tcttgatatc ttcataaatc attcgaataa atagcagttc ctccccactt
    14761 ttcttttctg cctgaagcaa aaacagctgg ccaaagctca ctaagtagac acagacagaa
    14821 gcgatcataa gcgctgtaag ggactccaac aaaacaaatc ctgttttatt caattctttt
    14881 tgtatagcga ccactcccta tctggaattg atacgtaatc gtccgctctt tttcttccca
    14941 ataaaattga taagctttta gtgaactttc attcccggtt ctagcagcaa aagtaataga
    15001 agcatggcgt ttcaaggtaa tctcatcagg aatatctaat acttcccaat ttattttatc
    15061 tggattcgga acatgaaaaa tgatttgatt ctcttcacta ttccaataaa agcctgtttg
    15121 gatctgattc acaatagcga ttttttgcgt agaatagatt cgtttttcaa attggccgaa
    15181 aaattgatag actgccaatt cattcttcca tgcagaaaat gcgagaactg gaaaagaaag
    15241 gatcgttgta agtaataaaa tcagtaagag tgattcaaac aacgtatatc ctgatgctaa
    15301 aaccttacct tcaaggccgc tgatagattt ctgcatgttc tcgtttgata tagccttcat
    15361 tcaacaattc ctccacagta ggaactcttc cattattttg aagcgcatag agctcttttt
    15421 gtgtgtctac caactggatg atcgcttcac gactttcttg gtctacatga ttcctataac
    15481 gcgacaaatt agggacgaac aacaacacta atacactgat gatcagtaag acgattagca
    15541 tttcgatcaa tgtaaaaccg ctataataag atatttttct ctttttcatg gcaaaacctc
    15601 ctctatgctt ccgtaaacag gtaataaaac agcggcataa actaataaaa tcaaagccgc
    15661 tacgcccaaa aaaatgattg gctgaataaa atgcatccag tgatgaagac gtttgaaaag
    15721 ctgttttctt gtccattcgc tgtaaaaaat cagttcttta cctaaacttc cctttgcctc
    15781 tccctgtaaa acaattttac tgaattcttc tgatagaaaa cgataattct ggaactgctc
    15841 agccaatgac atacctgcct ccaatccttg aatcaactta gaggcaagat gctgaatcag
    15901 actttcttgt ttcgtttcct ttaagcagac tatgatttgc cttaattcga aaccttcatg
    15961 aaaaagcttt ccaatctcta gtgacaaata agccgattga tacaaggaat atatcggtcc
    16021 aatcaataat tgtctgctta tccattcact ctgatcaaca ggagacaact tcatcagacg
    16081 ataccttact agtaaagcca atagagtaac cgctaggatg aatagtagaa cataccaatg
    16141 aaagacttgt atcagctgaa taccccaatg ctcttgcatg accattcctg aaccaataag
    16201 ctgaggcaaa acgaacaacc gcatactaaa caagatacct agcaaaaagg tgagcaatag
    16261 catcggatat gcaagcagct ttttcaaatc attgcgaaat ccttcaacaa gccgaaactg
    16321 tttggcaatt tccaacaaag ttgtcggcag attcccatga cgttcagcta actcgatctg
    16381 taccagttgc tctgctccat atcctatttg cgaaagtacc tcactaaacg cttgcccttc
    16441 ctttagctga tgttggatat ttgccaaaat aacaggtgga aacgaacgga tcgtgagtaa
    16501 taaataaagg gattcttgca gactaaaacc agataaaagc agttctccca tggtttgtgc
    16561 aaactggttt tgctggcttc ttgttaatcc tttagaatag ttctgactgc caaatatttt
    16621 cctgagcttc ttcataactt tcctcccatg atttttttac tgattcttcc tccataaatt
    16681 gataactcca taaaacagaa ggatggtgat tggcatcttc caaaagttct tgataaatga
    16741 caccagctaa acattcagat agctcttctt ttttctttgt cagttcatgg attctgacca
    16801 atgtaccttc aagattgcgt gcatgtacgg ttgcaaaaac ccggtgccct gttagtgctg
    16861 ctcttaccgc agcatgtgca gttgattcat ctcttatctc gccaatgatt agcaagtctg
    16921 gacgatgccg taatgcgagt ctcaaaagtg tatcataagt ctgtgctatt ttcggattga
    16981 tctgtagctg cagaaaaaac ggttcttcta tctctacagg atcttcgata gtaatcactt
    17041 gcaatttttc ttccaaagcc aaccgataca ttagagaagt cttgcctgat ccgactggtc
    17101 cgctgaaaag gtataatcca cgtctgacgg ttttctgtaa aatagttgca aaatctcgag
    17161 aactaaagca acgatggggt gtttcaaaag taccgtataa tagccgaatc actaaactct
    17221 ctcgttgaag gtaatctcct acagaagaca accgcagacg aacttcctgt ttttctaaag
    17281 aataagtaaa tgcacctaac tgagcttttc ttttttctcc gatatccatc tgtcccaaat
    17341 acttaaatct tgagatcagt tgctgtccat ctgccgaaga gactttcttc cagaatgttc
    17401 gtttttttcc atagcgaaaa tagatgtaaa atacttctga agctggcaat atatatacgt
    17461 cttgggcatg tttggcaaaa ctttcactta tcatttgatc cgataatcgt tttatttcca
    17521 ttattctccc ctccacaaaa aacatacgtc tttttgatct ttttgtttca aataaaatga
    17581 atttacaaaa aaatattggc aaaaaccgaa tttatgttat acttataaaa atttatcttg
    17641 ggaggcactc cattggaaac aacattaagg aaatatatcg aagtagcaag taaaaaaaag
    17701 cctgctgacc tagtgatcaa gcatgctaag atcgtcaacg tctttacgaa agaaatcatg
    17761 gaaaaagatg tagcaatctg cgaaggaatg atcgtaggaa tcggtgaata cgaaggaaaa
    17821 agcgtacata atgctaatgg gcaatatcta gtgccaggat tcatcgatgg ccatgtgcat
    17881 atcgaaagct cacttttatc accaagcgaa ttttcaaaag tatccttgct tcatggagtt
    17941 actactgtag tgacagatcc ccatgaaata ggaaacattg ctgggagtac aggtcttgag
    18001 tttatgatag aagatgcaag acaaacactc atgaatatct tttttatgct accttcttgt
    18061 gtacctgtaa ccccttttga gaccaatggt gcaaatttaa atgcaaaaaa attagcccct
    18121 tttttacaaa aacccgaagt tttaggctta gctgaagtca tgaactatca agcagtagca
    18181 aataatgaaa cagacatcct agaaaagatc caattgatgc accagcataa gaaaaaaatt
    18241 gacgggcatg cagctggtat cggcatggaa gagttaaatg tttatcctgc agcaggtatt
    18301 cggacggatc atgaagctac aactgcaaaa gaagctaaag aacgtttgga tcttggtatg
    18361 tatttaatgg ttcgagaagg gaccgttgca aaagatctgg catcgctact accggcgatc
    18421 acacctgaaa attcaagaag atgcttcttt gtcaccgatg ataaactgat caatgattta
    18481 gtaaaagaag gatcaatcga tcatatcgtc cgtcaagcta ttgcacttgg aatcgatcca
    18541 ttacaagcca ttcagatggc aacattgaat gcagcagaat gttttgattt gaaaacacta
    18601 ggtgcgattg cccctgggta tcaagctgat ttcttcttaa cagacgatct taccaagctg
    18661 ccaattcatg aagttttcta taagggaaca ttagttgttc aatctgggca actccaagac
    18721 catttatttg ctgatcattc aaattcatat acgtgttgtc tgccaaaact aaacgctcaa
    18781 ccattaaatc cacattcgtt agagctgctt ctttcttctt cacaagccca tgtgatcgaa
    18841 attatcccga atagtctagt gactaaagat ttggtagaaa aagtggaccg acaaggcgaa
    18901 cgattcattc cttcaatcga aaaagaccaa ttaaagattg cggtgatcga gcgtcaccat
    18961 caaacgggaa acattggatt aggcatagtg aaaggatttc agctgaaaca aggagcgata
    19021 gcgacaacga ttgcccatga ctcgcataat ctagttgtcg ttgggacaaa cgacgaggat
    19081 atgctgtatg ctgctaatct tttgattcaa aaaggtggcg ggatgaccgt tgtcaacaat
    19141 cagcaggaaa tagcttgtgt ttctcttcct ataggcggaa tcatgacaca ggaacctttt
    19201 tcgatcgtct atgaacagtt gaatgaatta ataactaaag cacatcaact aggagcagct
    19261 aaaacctttg atcctttttt gacactttct ttcttaacat tatctgtcat acccgaattg
    19321 aagatcaccg acaaaggatt attttcattt tctcgcttcc aattgattcc accttgtgga
    19381 aaaaccgatg aaataaatac attttaaatc aaataaatcc cagaaaatac attacatctg
    19441 taatgttcat tttctgggat ttatttatgg atattaatgt ttttatgaaa gcagaaagtt
    19501 agtttcacag acttttcttt ttggtcttat cttgaaattg agcttctact cgataaatac
    19561 gttgaccttt tgaagaaaat ttttcttcat actccgtcat aatattgcct tcaaattgac
    19621 tttgatgtaa atctaaccat acctgtttga ggatcatgcc atattgagaa aaacttgcca
    19681 gagaatactc aaacaatcct tgattatctg ttttgaaatg gatttctcca tttggacgaa
    19741 gaatctgttc atctacagca agaaaagtct tatatgtcaa tcgccgtttt tcatgacgtt
    19801 tttttggcca tggatcggaa aaattcagat agatttgatc tacttcgctg tcagcgaaat
    19861 actctgtcaa cgctgatcca tccacatgta gaagtttcag gttaggaagc ggttgttcga
    19921 tcaacttgtc tagtgcaatc gaaacaacac tgacctgcat ttctactcca atataattga
    19981 tttcaggatg ggcttttgcc attccagtga tgaactggcc tttccccata ccaatctcaa
    20041 tgtggattgg atgatcgttt tcaaacaatt cattccattt tcccttccac aatgttggat
    20101 cactaattac aaaattcggg tgagccgcta acatttccgc tgctcccgga cgatttcgga
    20161 cacgcattat ttatctccat tcctttcctc taattaaaaa ggtctggcca aatctccgca
    20221 gagtttgggc caaacctcag ccactgaaaa ttcgttttaa ttgtaaaatt tcttcgttca
    20281 tccgtcgatg atcttcacgt ttgcaatatc tggtgatttc ttggagcaaa ttcatggccg
    20341 cataccaatg aaatttttca agcatttcgt cagtcgggcg tatgccataa ctgatcagcc
    20401 actgattcca gctagacagt ggcacataat ttcctaaaat cgttgcaata tccaccgcag
    20461 ggtctgcaaa catgattgaa tcccaatcga ctagatataa ataattttta catactaacc
    20521 aattacgatg gtttacatct ccgtgtacta ctgataagtt ttccttatct attgacggga
    20581 tctgctcacg aagaaaacga tggacaattt gcagaaattg atttttctgg agttcttccg
    20641 gtaattcttt ttcatatttt tgcagcatct tctccggcgt catgacacga ccaccgatac
    20701 gggtcagcat ggttttaaga gaatttgaat gatgtaaatg gtacaaaaca tcaatcacgt
    20761 cgtttctttg accaatctca ttaggagtca gcaagcgacc ttccagccac tcttgtgctg
    20821 tcaaagtatc gccattcccc gttcgttttg tccaaacaag ttttggtgta attccttccc
    20881 ttgaaagagc agccaacatc ggagtcgtgt ttcgtttaat aaagactttg tcctcatcac
    20941 gcatgcctat gtatgttttt cctgtgtcgc ctttgatggg acgtaaccgc cactcttggt
    21001 ctaactgaaa ctccatcatg cattctcccg tcctcttact ctttgttttg cagtacttat
    21061 tattctagtc gcctgaacac tacacgtcaa gcacatatac gttctttttc atttaattgc
    21121 cattcaaaaa accagctttt ctcaaaaaaa atagaacgaa cagcattctt actgtcaacg
    21181 ctctattttt cattctcatt tttaccctac acagctttgt tttcagtctg acatcttctt
    21241 caaacgagtt ggtacatata gtttaacaaa gagcatagta acaatcaaat aagccgctag
    21301 cggaacccat cgctgctgcc catctaatac gactgcagca atcaaaccaa aaataaccga
    21361 cgcgataatt aatacccatg tgagtagata ctgaatcgcc tgcttcttct gattcttatg
    21421 gattggataa agctgaacga gaaccatata gcggaactgg ttcgctaaag gtaacagttg
    21481 gaaaccgatc aaatataaaa ataagaaacc gataatcaga ctaaaataaa agtcgactac
    21541 tccggcaatc aataacccac ctataattac taatcggaaa taaagaccac taaaatcaga
    21601 acctcttgcc aatcttcttg caaacaagta aagataagtg ttttttgatt ctcttttaat
    21661 atgagtcaat ataggatcca aatagcgtct tcttttgatc tttgccgtaa tttcaggaac
    21721 atctgtgaat aaattgataa attgatagat ctgatgcaac ctatgttctt ctacatgcac
    21781 cattttctcc caatccaatc ctttttcttg ttgcttccac aaaaaccagt aatatagact
    21841 tgcctgtact aatgcacctg ctattcctgc ccatattagc caataaaatg atagaaacaa
    21901 aaagatcaga gaagaaaaaa accagcctaa tttccaataa cgaataactt gttgatctag
    21961 tcgaaacaat tctgctctct tgatttgcag atgactgaat ttcaaactcc ataatatgat
    22021 cagatagacg aaaaacatcg aaaaggattg ctccgtacta accaccacta atggcatggc
    22081 caatccgcat gccaatatca aaaagatcaa cggaaaaatg caagaatagc gaaaagctcg
    22141 ttccaaatag gtttgcattt ctttttcttt aggaagtaaa aaaacaatat ctgctgcctc
    22201 tgcaaaagta gccatcttac ccacgaataa acagagcatc caaaatacag agattatgag
    22261 ccctccaatt gcaaacggtg gctgcagggt tttcaaccaa ttagaataat ataatcccac
    22321 accgccaatc aagaaaagac aaactaaaac aaaatgatcg ttcaatacat aacgcatata
    22381 tttcatcatt ttcttttgat gtcttgctaa acgtttttga aaaaaactac tcatgtgcct
    22441 gctcctttgt taaagcaata tagatttcgt ccaaagaaga atccggcagc tgaaattctt
    22501 ttctcaacat ctcaagagaa ccgtctgctc gtatctctcc gttatgtaag accacaaaac
    22561 gatcacaata tttttctgca gtagctaaaa tatgcgtaga catcaaaata ccggctcctt
    22621 cttttttcat ttctaccatc aattcaagca atgcattgat agctagtgga tccaagccta
    22681 aaaatggttc gtcgatgata tataaagaag gctcgatcaa aaaggcgcat aagatcatca
    22741 ctttctgttt cattcccttt gaaaaattag ctgggaacca gtcaagacga ttagataacc
    22801 gaaaagtctc caacaacttt tctgctcttt tcattgctat atccataggt atatcgtagg
    22861 ccatcgcagt gacttcgata tgttctctca atgtcaattc ttcatataaa gaaggagtct
    22921 ctggaatata cccaatttgt ttgcgatatt cttccgtatt tttctttaag gatagatgat
    22981 tgatttctat cgttcctttt gaagcttcca gcagaccgat aatgtgcttg atggtcgtac
    23041 ttttccctgc tccgttcagt ccaatcaacc ctactatctc tccaggctgt acatcgaaac
    23101 tgatatcttt taacacaggg agatgtccgt acccccctgt gacatgatcg ataattaaac
    23161 tcattctttt tcctccgcca tttattttta tttattatac cacgaagaaa atgacggtat
    23221 tttgtttttt tccttacaat agctttaact tatggtaaag ttaagctgaa gtatttattc
    23281 tttatagaaa ggatgtttta tatggaaaac tgtatttttt gcaaaatcat caatcaagaa
    23341 atcccaagct ataaaatata tgaagacgac aaggtctatg cttttttaga catctctcaa
    23401 gcaacgaaag gacacacatt agttgtgcct aaacgtcatg tagcagatat ttttgaatat
    23461 gatcccgaac ttgcaggaga ggttttttcg cgtgtgccta agattgctca agcattggaa
    23521 aaagcttttc cagaaatgaa aggtcttaat atcttgaata acaaccgtga actagcctat
    23581 caatctgttt ttcattcaca tatccattta gtccctcgct acagcaaaga ggatgatttt
    23641 tctattcact ttgtcaatca tcaagatagt tatggatcgg aagaactaaa agctattcaa
    23701 gaaacaatcg taaagcaggt gtcctgcgat gattaaaaac tttttcaaag gactgttatt
    23761 tggagctgct gctggcactg tcggaggtct gttattggcg cccaggagcg ggaatgaaac
    23821 aagaaagaaa ctgatcgaag aattggaaga agcaacaact ctgacaaatg atttaaataa
    23881 cagcctgaat cattttaaaa gcgcattgat cgaaacaaaa gaaacagctg aatctgtgat
    23941 tcctgctttc caagaagcaa tcaaaaaaga tatcgaagat tttaaattcc aagccgaacc
    24001 acgtatcaca caaatcaatg aacaagtaga aaaactaaaa gctgatctgc cagagtatcc
    24061 tgaacaaata taataatgat tattttctca acagatttaa ctatataagt gagaaaagtt
    24121 tgcgtatttc ctaggaatgt gatgaaattt tgaattttgt ttgaaactac cacgacaacg
    24181 tttttaaagg aaatgggata tggtatagtt aatatataaa aataataact aattacagga
    24241 gtgcaaattt taatgaaaaa aaaatcaatt attttggcag ctactagtgc gttggctgtt
    24301 ttaactctag cagcttgttc aggcgatact aataaagata tcgcaacgat gaaaggcgga
    24361 acgatcactg tatctgattt ctatgatgaa gcaaaacttg aatcatcgaa ccaatcctta
    24421 gtacaacgga tgatcattta taaagtattc aataacaaat atggcgataa agtaaccgat
    24481 aagcaagtgg atgcagaata cgacaaacaa gcgaaatcat taggtgatac ttttgaatca
    24541 caattagaag ctgctggata tacgaaagat acttataaag aatatattcg taacaattta
    24601 gcttttgaag ctggcttgaa agctcacgta gatattacag atgacgattt gaaaactgct
    24661 tggaaatctt tccatccaga agtagaagca caaatcatca aattatcaag tgaagatgaa
    24721 gcaaaagatg tgaaaaaatc agcggatgat ggagatgatt tctctaaatt agccaaagac
    24781 aaatcaactg atacagagac aaaagaagat ggcggtaaag ttaaattcga ctcaacgaca
    24841 acaactattc ctgcagaagt aaaagaagca gctttcaaat tgaaagacgg tgagatctcc
    24901 gatgttatta ctacaacaaa tcctacttct tatgcgacag aatactacgt tgtcaaaatg
    24961 gtgaaaaacc aaaataaagg caatgacatg gataaataca aagatcaact aaaagatatc
    25021 gctactgaaa ctaaactaag tgacaatgca ttcacaacta aagtaatcgg tgaagaatta
    25081 aaagatgcaa atgtaaaaat caaagatgat gcctttgaaa atgtgttgag tgcattcaca
    25141 acaacttcat caagtacgaa agactcaagt gaaactacag cttcaacaaa gagttctgac
    25201 acaaaatcaa ctgattcaac aaaagaatct tcaactgaag aaacaactga ttcatcaaaa
    25261 taaa
//
LOCUS       DAYYHX010000018        24634 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000018 DAYYHX010000000
VERSION     DAYYHX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.018"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..2187)
                     /locus_tag="ACHA6Y_000555"
     CDS             complement(43..2187)
                     /locus_tag="ACHA6Y_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8986671.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2544..3917)
                     /locus_tag="ACHA6Y_000556"
     CDS             complement(2544..3917)
                     /locus_tag="ACHA6Y_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8986672.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3917..4312)
                     /gene="rbsD"
                     /locus_tag="ACHA6Y_000557"
     CDS             complement(3917..4312)
                     /gene="rbsD"
                     /locus_tag="ACHA6Y_000557"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM8986673.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4340..5257)
                     /gene="rbsK"
                     /locus_tag="ACHA6Y_000558"
     CDS             complement(4340..5257)
                     /gene="rbsK"
                     /locus_tag="ACHA6Y_000558"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM8986674.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5365..6378)
                     /locus_tag="ACHA6Y_000559"
     CDS             complement(5365..6378)
                     /locus_tag="ACHA6Y_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM8986675.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6459..7058)
                     /locus_tag="ACHA6Y_000560"
     CDS             complement(6459..7058)
                     /locus_tag="ACHA6Y_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8986676.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7244..7945
                     /locus_tag="ACHA6Y_000561"
     CDS             7244..7945
                     /locus_tag="ACHA6Y_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8986677.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7955..10666
                     /locus_tag="ACHA6Y_000562"
     CDS             7955..10666
                     /locus_tag="ACHA6Y_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8986678.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11117..12745
                     /gene="treC"
                     /locus_tag="ACHA6Y_000563"
     CDS             11117..12745
                     /gene="treC"
                     /locus_tag="ACHA6Y_000563"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM8986679.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12805..13986)
                     /locus_tag="ACHA6Y_000564"
     CDS             complement(12805..13986)
                     /locus_tag="ACHA6Y_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8986680.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14238..15761
                     /locus_tag="ACHA6Y_000565"
     CDS             14238..15761
                     /locus_tag="ACHA6Y_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8986681.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15830..16558)
                     /locus_tag="ACHA6Y_000566"
     CDS             complement(15830..16558)
                     /locus_tag="ACHA6Y_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8986682.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16571..17851)
                     /locus_tag="ACHA6Y_000567"
     CDS             complement(16571..17851)
                     /locus_tag="ACHA6Y_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8986683.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17992..18594)
                     /locus_tag="ACHA6Y_000568"
     CDS             complement(17992..18594)
                     /locus_tag="ACHA6Y_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8986684.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18839..19045
                     /locus_tag="ACHA6Y_000569"
     CDS             18839..19045
                     /locus_tag="ACHA6Y_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986685.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19357..20076
                     /locus_tag="ACHA6Y_000570"
     CDS             19357..20076
                     /locus_tag="ACHA6Y_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8986686.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20157..20840)
                     /locus_tag="ACHA6Y_000571"
     CDS             complement(20157..20840)
                     /locus_tag="ACHA6Y_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM8986687.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21058..23244
                     /locus_tag="ACHA6Y_000572"
     CDS             21058..23244
                     /locus_tag="ACHA6Y_000572"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM8986688.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            23590..24000
                     /locus_tag="ACHA6Y_000573"
     CDS             23590..24000
                     /locus_tag="ACHA6Y_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8986689.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            24014..24370
                     /locus_tag="ACHA6Y_000574"
     CDS             24014..24370
                     /locus_tag="ACHA6Y_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8986690.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         8223 a         4388 c         4812 g         7211 t
ORIGIN      
        1 aaaagggagt gtgacagtgt cacatcccct ttttaattta ttctatttct ttaaaggcag
       61 atcaagccag aatgatttgt ctaaacttaa caaggttagt acaggaacag ttgcagcttt
      121 aactgtacga gtagttgttt ctggtatctg tcttgccgta aaattgttcc acaaatccca
      181 gattgaagcg acacggaaga tggaagaagc gttgttgacg cgttttatcg ttgaaaattc
      241 taatcctgtg attggcacgc catctgttgt ccatttgatt ggcacatcga atcgcttaga
      301 catattgagg atttcagcgt ttgcttcttc agttgttgct gcttcttttt tcggttcatc
      361 atattttgtc aggtcgtacg cttcgatgat cgcgttcaat ttttgtgaat attggcgatc
      421 tgtagcataa cgtccttcca aatatttggt tgcttctttg tagtcttttg tttccgactt
      481 ccaagttggt ttgtagaaat caggattgcc agtcagtcca tttttgatca agtcggcata
      541 gtcttccaaa ctttccttgt aagaaggata tttacggaat tgtgcgcgga tttggtagct
      601 ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg ctgcttcctt tatatgatcc
      661 tttgatacca aaaaggttat gatttggttc gcttgataat aagctgtttc cagatccact
      721 ttcaaggata gcttgagcca tcatgactga tgcgtagatt ccttcttttt gcccgatttc
      781 ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt gtttggtttt gactgaaatg
      841 gaacgagcca tctaatccat ctaaaataga tgagccgcct gtcagcgaac ctgaagcagc
      901 aggtaaacct gctaagttta ctcggacagc aaaatcaggc gtgaaatctg aaatagaagt
      961 tactcgaact ttatctcctg gttgaggcgc atggataaat tgtccgttcc caatatagat
     1021 acctacatgg tgagtaacac cggaagaagg tccccagaag acgaggtcgc caggttgggc
     1081 ctgacttact ggaatctgcg tacctgcata ttgctgttcg cctgtccagc caccgatata
     1141 atgaccggtt gctttcatat atacgtaata agtcaaacca gaacagtcaa aagtatttgg
     1201 accttttgct ccccatacat atggttttcc aagatgcttg aatgcttctc gaacgattgc
     1261 ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca attggtgatt ttacaacaaa
     1321 atcagaagaa gcagaagatg gattctctat accgctttga tttgctgtat ttggacgaga
     1381 tgaagcattg ttcgttccgc cattcgatga accattgttt ggatggtttg gttctctctg
     1441 agtatttcca ttgttattcg atccagatgg gtttgtttga ttcgaagaat tcccgctgtt
     1501 cgaattattg ttattattgt ttgaattatt tgaaccatta ttattgttgt tattacccac
     1561 atctggtttg tttggcgttt ccggatctgg tgtactaggg acttcttcac taggatcagt
     1621 tggtttctct gttgaagtat ccggtccagc tttaaattct gcagtaatag ataaattgtt
     1681 cgtacctact gtaaaggtct ttgcatcata atttacttct ttggcaccat taacagcgac
     1741 tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa gttaccttcg tttccgctaa
     1801 taaaccttct accgttccac tttccgtaat atccaaaggt tgctgttcag gggaatccgc
     1861 tgtcgaagca gtcagttgga tcttaccaat caaagaagga gcaattgtaa gggaatatgt
     1921 ctcggctggc ttttcatttt ctgatggatc agccggatca atttcagttg gctcggtcga
     1981 tcctgttgtt gagttagttg ttccagttgg gtcttctgta aaagaatccg ttgtttcact
     2041 agtagctgag ctggatgtag tcgtatctgt tgtgactgtt tgatcagcta tttgattatc
     2101 tacggcaaat acggtattgg tcgacaaaac agacggagca atcagccaag tagcagctag
     2161 caaacgtatc gtttttttat ttttcataat aaactagtcc ttttcctttc attgattttc
     2221 tgtcttattc agcattcatt agacattttt tggaacagat aatcgtgtcg aaaagctttt
     2281 tcttttcata aatctgtctg tgtattctaa ataaaacaat ttgtaaataa gcaacgagca
     2341 ttcattcgtt tttctttgac gttcatttta ttgcatagac cacctttgtt tggggataaa
     2401 caagtgggga taaaaatggt tatgcatatg taaattatac caaagtttgg aaagtatcac
     2461 aaaaatttat aaaaataatg atcctaaaaa gaaaaagact gaaatatgtg ataaacatct
     2521 catacttcag tctttttcat taactattca taagacatta ggtcttcatc aaacccgcta
     2581 acgagagtag tgatcattgc tacgactaga ctaacagcca gtcctgctaa gtaccagaaa
     2641 aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac caaatacaaa agcgatcgct
     2701 ttcacttggg caaatcccat tacagctcca ccagcagctg caccaatgca agcagcaaca
     2761 aatggttttt tcaatgggat cgcaattccg taaacagttg gttcggtcac tcccgcaagt
     2821 aaaatcgaag caaaagcaga accggagatc gaacgggttt ttgaacgttt acttcgcaga
     2881 aatacaccta aagctgcacc agccattcca aaattggctg ctcctgtcaa cggaacgatt
     2941 cggtcgaacc cataagcaga gatattactg atcatgacag ggtttacagc ccattggata
     3001 ccaaatatta ccaaaatatt ccaaccacca gcgatgattg cgccactgat cggtccgtta
     3061 atatccatca tccaagtaat tgcggcagct aaaccttcgc caaggtaaac acccgcaggc
     3121 ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg aaagtaaagg tacaaatact
     3181 aattgcatat tttcgcgaat atattttttg aggaattttt caacaaaaga ataaaagaaa
     3241 atacctaaaa tcgccggcat gacagtagac gtatactgca tcagtactac aggaatcccc
     3301 agaaaatcag tagctgcttt gccactgtct gaaagtaaag aggtcagatt cggttcaatc
     3361 agtgctgcag caatgacagc cgcaatatat ccattcactt taaacgtttt ggcagcagaa
     3421 aaagcaagaa agattggcag aaaataaaag acggcattcg acgcagcaga caaaatatga
     3481 taagttccgc ttgtttcact aagccagcca atcgtagtcg ctaaaacaac cagacctttt
     3541 aatataccag aaccggcaaa cagcggaatg atcggcgtaa agatttccga tatcaaacca
     3601 aatagttttt cgatcggatt tagcttttct ttcggagctg aatgatcgat ttgacgcgtc
     3661 aattccttgt aagcaggagc caatccctga tccatgatta tttctgcata tctatcgagt
     3721 aaaatgaaat acttaatgga agggatttgc tgttttaatt tttctgcatt ggctactggc
     3781 tgttttttca atttgatacg aagccgagtc gcacatggaa ggacagaatc aatattgtct
     3841 tttcctccca actgttcaat cagcatatga atctgctcgg acagtaccat ctgttcagga
     3901 ttcattgtcg tttccattag aagaccactc cactcactaa tataatattg gcgtaaggtg
     3961 tcatttcgcc tgtacgaata aatgcatgac agttattcaa ctcttttttc atttcttcat
     4021 gtgggataaa gctaatcggt gtatctggta acaataattt gattttttcc aatatttccg
     4081 ggttcattgt ctttatttct tccgccaatt caatcgattg tacctcaagt tcttctaaaa
     4141 cgttttctaa aacagataaa aaacttggaa gaccgtttga gacagccaaa tctatttttt
     4201 cagtaccaaa ggggacaggc atgcctgcat ccccgatact taatttatca aagtgcccca
     4261 tttgtgcgat tactcgtgaa atatctgaat tgattacttt tccttttttc atgtttgttt
     4321 cttcctctca ttgttaattt tagactgctg attgttctaa agcttcgcgg taagggatag
     4381 aaggttgcgc accataagac tgcacagcaa cagaagaggc tagatttcca taaaggatcg
     4441 cttctttcaa gttactaagg tctggctgaa gtgtgctgct taatgcgcca ataaatgtat
     4501 cacctgcggc tgttgtgtca acggcatcca ctttaaaagc agggacgata ccgtgttctt
     4561 tttctgtatg atagaaagcg ccggcactgc ctagtgtaat gattactgtt ccaataccta
     4621 attcatggag tttttcggct gctgcgacca aactgttatg atctgtcact ctgattcctg
     4681 taatgatttc tgtttctgtt tcatttggta taatgatatc tgtcttttca agtagttcag
     4741 taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt tgtttttcct gcgtcttttg
     4801 caatagaaaa agctaggatc gtcgcatcta agctactttc aaactgagca ataataaagt
     4861 cactgtttgt aatcagttgc ttgtttttta ggacttcttg ttcatggaaa gcattatttg
     4921 cccctgcatg gatcaagata ctattttctc ctgctgcgtc taccataatc attgcttgtc
     4981 cggttgtggc gccttggatt tcagctatcc cagatgtatc gatgttttct tttgtcagta
     5041 gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc tccaataaaa gatgtccgtg
     5101 cgcctgaacg tttggctgcc actgcttggt tggcaccttt acctccgcca gcgtgaaaaa
     5161 tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt ggttaaacga agggtagtgt
     5221 ccatattaat acttccaatt actgtaaccg tgttcatttt acaaacctcc aactatctca
     5281 tttattttga tgggaaaatt attttttaaa acgttttagt aaaacgtttt aatagttcga
     5341 taaaaatata gcacaccttg aaagctattt caagggtgca gttgaaaatc ttttttctaa
     5401 tcgtacaggt aatctttttt cttcccattc tttttctggg aattggatcc gatctaacaa
     5461 taacttggca gatgtttgtc ccaattcgaa gattggttgt gcgatcgttg tcagtttcgg
     5521 cttgatatac tcgcacatat ctatattatc atagccaata atgctgtagt cttcagggat
     5581 gctttttcca gcttcctcta atccgcgata taatccaaaa gctaattcat catttaaagc
     5641 aaacacacca gttgcagcag aatcaagaag ttctgctgtg atctggtagc caccttgttt
     5701 tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg atttggtaaa catcaagagc
     5761 agatttaaac ccctcaatcc gagcgtggac attttccggt ggattttcag gatagacaag
     5821 ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc gctaggtaac caccgcgaaa
     5881 atcgtctgtt cgtacggcat cactaaatcc ttcagatttt ttctgatcca aaacaatgaa
     5941 gggccggcct tgttttttca aattttcgtt gattgcatct gtagagacag cagaagtcgc
     6001 gataatgaat ccatccacac ctcgacgtgt gagctcggct agatattcga tttctttttg
     6061 atgatcagaa tcagcattac ataatatcgt gacgaaattc tgcttataca agatatcctc
     6121 aattcctcgc atcagtgtac tgaagaatgg attcgtgata tcaggaacga gtacaccaat
     6181 tgttttcgtt tccttggtaa tcatttgtct agcaaaatag tcaggctgat agcctaattc
     6241 ttctttggca gctaaaactt tctgaacagt tttcggactg aatttctccg tatttccatt
     6301 taatatcaag gagacagttg tgattgaaac gccggaaagt ttagcgactt ctttgattgt
     6361 gatctttttt ttcaccatgt tcatactcct atctaaatcc tgtggttatt ttcccatgaa
     6421 atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt atttgctttg ttggatagct
     6481 tcgattattt gtttcgttga gcgtactcgt gcagttttcg gaaatatctt ttttatgaac
     6541 aattgatggg attctttgtc acgatcactg caagcatctt cagcaagcac gatatggtac
     6601 ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt ttgttgtcgc tacaccgctt
     6661 aaaatgatag tttcgattcc acgccggcga agttgaagat caagagaagt accaaaaaaa
     6721 gcacttggat tgtattttgt gatagtcaac atattatctg tatctttcaa agaatccttc
     6781 aaaagtaaat cagtgaattc tggaggtaca gaagcagtca ttccacccat atcagtttgt
     6841 tgactcaaat aagggaaagt ggagatgtct acgttcacta aaactttcaa ggcagcggta
     6901 cgtttcaatg ttcttactaa caaatcattg tttgtcagaa tagatgctgc agtgtgtgga
     6961 gagagtgtac cgttatttac gatctctttt tgcatgtcga tcgcgacaaa agcagttttt
     7021 gataaatcaa aaatcatatt ttcctttaaa tcattcatga tgttccttct ttcaaataac
     7081 aatgtttttt atgtaagtaa atcataaatc gtttatttgt caactaaatt aaaggattcc
     7141 tttctttagt ctagagaaat tcagtggtaa aataaataag tgtgagaaaa cgcattcgat
     7201 gcatagatat agcgagaaat atcgtgaaaa tggaggaatc agagtgaagt atgtcgaagt
     7261 aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc attacagcaa atgacgatat
     7321 ttctttttca attgaaaaag gcgaattagt gattatactt ggtccaagtg gagcaggaaa
     7381 gtcaacgatt cttaatattt taggtgggat ggatacacct gatgaaggtc agatcattat
     7441 tgatgacaca gatattgcgc aattttcaga taaacagctt actgcttacc gacgaacaga
     7501 tgtagggttc gttttccaat tctacaatct cgttcctaat ctaacagcaa aagaaaatgt
     7561 ggaattggca acggaagttt ctcctgatgc gcttgatcct gtagaggtat tgaggcaagt
     7621 gggcctagct catcgattga ataactttcc gtcccaatta tcaggaggcg aacagcagag
     7681 ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt cttttatgtg atgaaccgac
     7741 aggcgcgctg gattttgaaa cgggcaagca agtattgaaa ttattgcaaa acgctagcag
     7801 acaacacggc aataccgttt tgatcattac acataattct gctttagcgc ccatcgctga
     7861 tagagtcatt cacattaatg atgctaaagt aaggtctgtt gaattaaatg atcatccttc
     7921 atccattgat gagattgtat ggtaggggga ttttatgaaa acaagaacat atcttaaagc
     7981 aggttttcgc gaaattcggc aatctaaagg acgattcatt gcaatcatcc ttattgtcat
     8041 gctaggaaca cttttatttg taggtgtcaa aactgctgga cctgtcatgc aaaaaacgat
     8101 ggatcactat gttggtacag caggactttc tgatttgcaa atcgtttcta ctggtggctt
     8161 gacacaaaaa gatattgctc aagcagaaaa gatcccagat gcaaaagtag aaacaggaaa
     8221 acagatctat tattccaata ctggcaaaaa cgaagtgatt caaatatttt cttataacaa
     8281 aaataacaag cagaacaagc tacaattaac tgatggaaaa ctcccggaaa aaccaaacca
     8341 gcttgtattg gatgaaaaag cggaagatga agggtataaa atcggtgata tttatcagat
     8401 tgattcagat gaattgaaag aaaaagagta taccatcacg ggctttgtcc gttctccatt
     8461 gttcatcaac aatttggaga gaggatacgc gaacgtcgga aatggtagcg tcgattattt
     8521 tgtctattta cctgaatccg tttttaaaac agatatttat tctgtcttat atcttgattt
     8581 tacaaacgta aagaatttga atacgtacag caaagcttac aaagataaaa tggagcaaaa
     8641 tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa gaacgcttag aagagctgaa
     8701 aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa atagcggatg gcaaagcaca
     8761 aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa cagttagcgc aacagtcagc
     8821 aactatccag cagcttcctg aagcgcaacg aacaatcgct gttcaaacat tatctcaaca
     8881 agaagctcaa cttgctgaac aagaaaggca attagttgag aaagaacgtg aactggcctc
     8941 tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa aatttaacta aaccatcata
     9001 cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa tttggtgatc tgtctgatcg
     9061 tatcgctgcg attgccaatg tttttcctgt tttctttttc tttattgccg cattgatcac
     9121 ctttacgaca atgacacgta tggtagaaga aaaccgcagg gaaatcggga cattaaaggc
     9181 gttaggttat acgaaagttg aaatcgcaaa aaaatatgca atctatgcaa gtctagcctc
     9241 agggataggt attgttttag gaacgatatt aggaacgaat cttttgccaa ggatcatttt
     9301 tgagctgtct aacgagcggt atgatattgg gagtgcgctt attttttatg actggccgcc
     9361 gattttacaa gcagctgccg cattcttcat tgccgcattt ggcgctgcaa tgatcgtttt
     9421 attcaaagat ttaagagaac ggcctgctgc attgcttcaa ccgaaagcac cgaagccagg
     9481 gaaacgcatt tttcttgaat acatcactcc gctgtggtca cgccttagct ttaatcaaaa
     9541 aatcagttac cgaaatctct tccgatataa gtcaagaatg cttatggcga ttatcggaat
     9601 tgctggatgt gccgggctga tggttgcagg cgtgggattg aaagattctc taagttctgt
     9661 ttcagccaaa caatttggac cgatcattga ctatcaagca atcgttacat tggattcatc
     9721 cacggaagat agtcgtcaaa aagcagcgga tatgctgcag aagcaagaaa aagtaaatga
     9781 gatcctagct attcatactc aaaccgtaga attgagaaaa aaaggacatg cttcgcaaag
     9841 tctgacagtg atcgtaccaa gtaaaacagc tgatttggat cgttttatcc atttaactga
     9901 tcttgaagag aacccacgct ccttgcctaa aacaggaatc gctattacgc aaaaagtagc
     9961 agaactattt gacttatcta caggagatca tatttcgctt tatgatgaag atcaaaaaga
    10021 atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta ggtagcttta tttatatgtc
    10081 ccctgaatat tatgcttcag ttactgatca atcgatgatg gataatgcat ttttgctgcg
    10141 tacagataag atgacaacaa aagaagaaga aatgctttcc gagaaactgc ttgccacaga
    10201 cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa acgcaagaag aatcaatggc
    10261 gaacttagac tcggttgtct taatctttgt cgtattatca ggactgctgg cttttatcgt
    10321 cttgtataat ctaactaata tcaatatttc cgagcgtgta cgcgaattat ctacaataaa
    10381 agtccttgga ttttttgata aagaagtcac gatgtacatt gttcgagaaa atatcatctt
    10441 tactctttta ggaatcatcg gaggatttgg tgtaggatat atattgactg acttcatctt
    10501 gcaacaagcg tctatggaga atgtcatctt tccattagtg atcacatggt gggcttatgt
    10561 cttatcagca ggattaacta tcgtatttac ggttattgtg atgattgtta cccatttcaa
    10621 attaaagcat atcgatatga ttgatgcgtt gaaatcgaat gaataaaaac agaggttgtg
    10681 aaatcagcgg tttgacctga gtattagaga aacaaacggc aaaatagttt ttcatatttt
    10741 gccgtttttt gagctaatac cgaaggacag ctgattgaac accatgaatc agaggttgta
    10801 aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata aaattagctc ctcatgtttt
    10861 acatttgaga agactgattg ccgtagataa aagcttttga acaccattta tagaagaaac
    10921 aagaatattt tgctatttta aacaagagga tagacaaata catgtctgat ctcttgtttt
    10981 ttattgggca acagacctct tagaatgtat aaagataaat tttacaaacg ctttcggttc
    11041 agttataata ataaatgata acttgtatat ataagttgtg gtaggtaata aatcagttga
    11101 atggaggtaa atatccatga catttaaaga caaagtcatt taccaaatct atcccaaatc
    11161 ttacaaagac agtaatggtg atggaattgg agatttaaaa ggaatcaaag aaaaactccc
    11221 gtatctgaat gatctaggca tcgacatgat ttggctaaac cctatttatc caagccctca
    11281 aaaagacaac ggttatgata tctctgatta cacagccatc gatcctatct ttggtacgat
    11341 ggaggaattc gaacagctaa tagccgaagc aaagaaatac cggatagaaa tcatgctcga
    11401 tatggtgctg aatcacgttt cgacagaaca tgaatggttc aaaaaagcac tagcagggga
    11461 ccagcattat caggacttct tcattttaag agaagagcca acggattggg tatcaaaatt
    11521 tggtggaagt gcttggtcgc catttggaga tacggggaaa tattatttgc acttatatga
    11581 tcggacacaa gctgacttga actggcggaa ccaagcagta agagaggaac tattcaaagt
    11641 tgttcgtttt tggatggaaa aaggggtaaa aggattccgt ttcgatgtta tcaatgttat
    11701 cggtaaagac gaacaattga aaaataataa agaaaatgat ggcaaacctg aatatacgga
    11761 caaaccaatc acccatacgt atctaaaaga attgaaccaa gcaacgtttg ggacggattc
    11821 cactatcatc acagtcggtg aaatgagctc tacgacgatc gaaaattgta tcttgtatac
    11881 agaacctagt agagaagaat tatcaatggt ctttaatttc catcatttga aagtcgatta
    11941 tgaaaatggg aataaatggt ctactcctcc atttgatttt gcagaattaa agcgactatt
    12001 tcatacatgg ggagaaaaga tgagtgaggg taacggctgg aatgctctat tttttaataa
    12061 tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc gatcagtatc gaaaacaagg
    12121 agcagagatg ctagcaacca tgatccattt gaaccgtggg acgccctata tttatatggg
    12181 agaagaaatc ggcatgatcg acccagacta ttcatcgatt tccgactata ttgacgtaga
    12241 aagtttgaat gcttataagt tactccttga acaaggattc acagaaaaag aagcgtttcg
    12301 tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc atgcaatggt cagacacaga
    12361 acaagcagga tttactacag ggaaaccttg gttaaaagtt ggaggcaagc tagaagagat
    12421 taatgtagaa aaagaactat cttctgaaga ttctattttg tcctattaca agaaattgat
    12481 tcggctgaga aaaacatatc caatcgttgc acaaggagat tatcatgctt atgaagcaaa
    12541 tcatccggaa gtttatggct ttcttcgcca atttgaagga cagcagctgc ttgtattaac
    12601 aaatttctat gataaagata caaggatcgc catcccagaa ggattcacag aagccaatat
    12661 tcttatcgat aattattccg atactgttct tcatcaaagt ctggagctaa aaccatacca
    12721 ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa cgtcgttcct acgagctatt
    12781 ctcgtaagat cgacgttttg tgttttattt aaaataatct tcttcagaaa atgaagagtt
    12841 ttcatactct cctttatcgc cataaaattt attgtagcgt tgtttgaatt tacggaaaat
    12901 aaaagtaacg agtaatttga ctaacgtata gataggaata ccaaaaataa cgcccacaat
    12961 tcccattaga tcacccatga caagtaaaac gattaagatc gtaatcggat ggatctgtaa
    13021 acgatcaccc attactcgag gaatcaccaa gtcaccgtgg agaagttgta cggcaaacca
    13081 gacaatgaca aatttgacag ccataaaagt agagtcttgg aatgcaatga tcaaacctgg
    13141 aaagaaggcg ataaacggcc caatatatgg aatgatacat aacaacccag cagaaatagc
    13201 taaaacacta gcaaattcta atccgatcag taagaaacaa acccagtaga ctgctccaag
    13261 gataaatgaa gcaatgattt gcccctttag aaatgaaccc aattgttgat tagcaatctt
    13321 tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca atcgctaaaa ctgactgata
    13381 aaatttttga ggatctctca aaaggaaaaa agcgacaatt ggaccagtaa ataaagtaat
    13441 aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt tgtgcgccac tttcccaata
    13501 atttccaata aatccagcta tatcagacgt cacgtcgtca atagaagcga aaaattgatt
    13561 aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg agactctctt gaaaatcttg
    13621 aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt tcttcaagaa aaggaataag
    13681 ccatatgctt ccaagcccca ataaaaataa aataatcaca taaacgataa tgactgacaa
    13741 gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt tccaaaagat aatacaagat
    13801 cagtgcaaac aataatgggg gcaagatagt agcgataatg attccgacag gcttgaatat
    13861 aaacgagatc tgatggaaaa aataaatgat cagcgcaagt aagataacta ttcctaaaac
    13921 atagtaagag gtttttcctc ctaaaaagcg gatcaaacga ttcgttgtat ctgtttcttt
    13981 tttcactctt tttcctcctt tatctataac ttcattatag acaaaagcgg gtataaaagg
    14041 aaaatgatag aggttgattg caaaaatatc gtcaatcatt catataaact ttctcatcaa
    14101 ttaaaataag aaaacgcttg acagttcttt taaccatatg ataaattaaa tcctgttgct
    14161 aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac attcaaatga aagaagatga
    14221 gaagaggagt gtcataaatg aaacgattga taggtatatt ggccatgctg gttttagcag
    14281 gtatgttaac tgcttgcggg gcaagtggga aagccgagga cagtaaagaa aaattgagcg
    14341 tgatgacgac cttttatccc atgtatgatt ttacaaaagc aatcgtcggg gatgaaggag
    14401 aagtagagtt attgattcca gcagggactg attcccacga ttatgaacct tctgctaaag
    14461 acatggcaaa gatccaagat acagatatct ttgtttataa tgatgaaaac atggaaacat
    14521 gggtccccgc aatccaaaaa acattacaag aaggcaatgt ccatacgata aaagcgacag
    14581 aaggtatgct tttacttccg ggaagtgaag aaggacatga tcatgaccat gaacacgggg
    14641 aagaaggcca tacacatgag ctagacccac atgtatggct agctccaagt ttagcaatca
    14701 agcaggtagc aaacatacgt gatcagctga ttgaagctta cccagaaaaa caggaagtat
    14761 ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc actccatcag ctttatcaag
    14821 aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca acatgctgca ttcaactatc
    14881 tagcgttaga atatggattg aatcaagtat cgattgctgg tctttcttct agtgaagaac
    14941 cgtctgcggc gagaattgct gaactgaaac actttgttaa agaacatgga atcaactata
    15001 tttattttga agaaaacgcc aaagatagta ttgccagaac gttagcaaac gaagcaggtg
    15061 tatctttaga agtattgaat cctttggaag gattgacgaa tgaacaaata gaaaatggag
    15121 aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt gaaaaaaacg acagagacag
    15181 aaaacccgct agaagatact ttgaccccca aaaaagaaaa gagcgtctac aacggttatt
    15241 ttgaagacga ggatgtacaa gatcgctctc tttcagattg ggcaggaact tggcaaacag
    15301 tagatacgta tgtagaagat gggacttttg atcctgtttt tgaatataaa gaaaaattga
    15361 atcaggataa gacagcacaa gaatataaag aatattatac gaagggctat caaacagata
    15421 ttgcttcaat caagatcagt gagaaagaga tggcatttac gtttaagaat ggaacaacga
    15481 aacgttcaaa ttatcgttat gtagggaaag aaatcttgac ctacgaggca ggaaaccgtg
    15541 gcgtaaggta tctattcgaa gcagaagata aggaaagtgg cgcttttaga tatattcaat
    15601 tcagtgatca cacgattgca ccggctgact ccgaacattt tcatttatat gcaggaaatg
    15661 aaagtcaaga agtattgctg aaagaaatgg agaattggcc aacattttat ccagatacaa
    15721 tggatggacg tgaaattgca caggaaatgc ttttgcactg ataggcaaaa aagaaccgaa
    15781 gggtttggat cggctggtca tttagtcggt ccaaactatt cggttctttt tatgaaatca
    15841 atttctttaa tccacggata gaggctggga tggtctcgcc gtttgccaac gtgacttctc
    15901 tggttttttt attaatcgac cgcacatttt gtgtattgat aacataggat ttatgacaac
    15961 ggataaaatt ccgatgcatt ttctcgattt ctttgatctt ctcatagaat tgtaattggg
    16021 catgagtcaa atggacttcc agtttatgtg gcgctcctag cgaagtagaa aaatattgga
    16081 tgtctttgat cggtaaaaat ttgatctcat cattaaactc tattttgaag atatcttttt
    16141 ggatttggga catttgaaaa tcttcgaaaa ttttgctcag gatatgctcg acattttctt
    16201 ttaaatcata aagatcttct ttgacgatat aatcagtcgg ttcgatgttg ctttttaaga
    16261 ttttcatcga catttccgta tgggaagtca catagattat ttttcctaaa ggatctaatt
    16321 cacggatcaa tttaccgatc gctacaccat ccatttcact gtcttctagt tcgatatcta
    16381 aaaagtaaat accaaaagaa gaagttctga tcttggctgc attgagaagt tcttccggat
    16441 cagaggtaga aatatggaca taactgtcta agttttcaat catgattcta tttttgatca
    16501 ccgttgataa aacttctaat tgttttggat cgtcttcaca aatataaatg ggaatcatat
    16561 cattactcct ttattttatt tgtatataaa tcgtttgaga aaaacgctgg ttttcaattg
    16621 atgtttcaag gaaaagataa ggattggtct ttgtcaattc atccaagata aacaagccaa
    16681 ggccttcgtt ttttggattt gtttttgttg agaacccttt ttgtttcaac tggtttaacg
    16741 gtaatgtatt tgttgttgta ttgatcacac gaatcatcac ggaatgattc ttcttgagta
    16801 tggtcaattc gatttttttc ttctttgcag cactagctcc ttcaatcgca ttgtccaaga
    16861 taataccaag catacgtaca aggtttaccg tatgtttcgt atccagttcg attggctcag
    16921 gaaaatgaag ctggacgatc aacccttttt cctgtgccat catgagtttt aatgacagta
    16981 aactacggat ctcaggaact ttcatattat ccaacatact tagattggcc ggaaaaaggt
    17041 tcatcatctg gcgtgtaggt aatatcgttt gatcataata agaccgtaat ccttctaagt
    17101 cgccttctga aatatagctg tgaagagaaa aaagcagatt ttggtaatca tgtttgaatt
    17161 ctctcaagtc ttgatagttt ttttctagat ctttgatata atcctgttgc atcctgactt
    17221 gttgctctaa taactgatca gctaatttct tattagagat tttccaataa taaaccaaga
    17281 tgatcaggcc aacacacaga tatagaatga aaaaactcgt attcaaagct aagacatcag
    17341 gtgtttcacc agaaaaacgg gtataaaaaa taatgatata gtatgtaaat aatatcaatg
    17401 tcccgatcgt accgatcata cggcggtcca cagtgtgaaa ccaagtctct aacgattttt
    17461 ttaaacaaaa agctaataaa gcagataaaa aagcggataa gacaatgtga aaatactgca
    17521 atgaatctcc tggttcaata accgaagatt tcaaaacaaa aaaatcgccg atgcttgtta
    17581 agtgatctcc taaaagcgac gtcaataacc caaaaggaag cgcgtaaacc aattgttgac
    17641 gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc tatagcaatt agagcagtaa
    17701 aaatccctaa aatcgaatag acccatccaa tcaaaataca agaaatcaga atgactcccg
    17761 caaacagata tttgtatcgg atgtgatggt caatcaataa gatacaaatt gtgtaaatca
    17821 aatattgcaa cgctatggac cataaaaaca taaagtacag ctcccattga tttttaccaa
    17881 attatagcac aattggcgta ttattttttt aatttttcag ggattttgaa ttcagaactc
    17941 ataccaaagc agcagccaga tacaaaaaag tcagataagt tagttaattg tttaatgatt
    18001 gtttttttca tgaaagattc ctccaaaatt catcaagtag tttgtaatag gaagcaacgt
    18061 agctatttct aaaacaatcc caagtagcaa ataggtagaa aagtaagaag ctgagaaaaa
    18121 taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc ttctttaata atgggggacg
    18181 tttctcgggt tcaatgggat tgattgctgt acctcttgat ccatagcgaa gcagtaacaa
    18241 aaagcagctg attaacaaaa agaccatcat tgggaaggag atagtcaatt gcagatgttt
    18301 catcaccaag ggaacgcccc ataaataaag tagtgtccac agcagacaaa cgaattcaga
    18361 gtcggaatgc aatccataag catatctgcg gatgcagata taagaaagaa aggtgattcc
    18421 tgtttccgcc catgagcctg tcaccagaga aaagactaga acgaccgcta gttttccaag
    18481 attattcaat aaaacttcta agacaaattt tacttggaca tacaccatat cttcttccga
    18541 tttgtctccg ctgaataagg cttctaataa tttgttcaca actttatctt tcatctcata
    18601 cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa acgtcttttg tttcataaaa
    18661 agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa atcttcgttt tccaagaacc
    18721 ttaccaaaaa aagtgacagt tttgatacga aaagtacttt ttatgacaag tttgtgcttt
    18781 ttcgagtgat ttatgttaaa aaattactga ccttataaaa ggtgatggag ggattcttat
    18841 ggaaaatgaa caattgtttg aagtcggcga aacatatgca tttaaatctg atttatttga
    18901 atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat agtgtgatga tacgtgtcat
    18961 gaaatgcgca gtagaagaca atgataccgc tcataatctg cactggcagt tagttgtacg
    19021 caaatcaagt acaatagaag aataaaaaca gataaaatgt tgagagcata aagaggttgt
    19081 gagtctcccg tttagctctg agttacaagg aataatgtta aaaaacagct tttcatgttt
    19141 tatgaatatt gccttgtgac cgaggtgttg gcaggagaat actgtttatt tgaaaaaagg
    19201 gctgcgacaa gacttttgtc acagccttta ttttcatgaa taggagttac ttttggctac
    19261 ttttagagaa gtagctttta aggagcagat aaagtcaagg cttttatttc tataaatgaa
    19321 gtagtaaaat gaagaacaga ttttattaca ggaaggatgc cgataattgt gatcattgtt
    19381 gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa tgcttgccca agatttagaa
    19441 acaaaacctt tttatgagaa cgtggaagat aatgaagtat tgccgctatt ttattctaat
    19501 ccagaaaagt atactttttt attacagatt ttctttttga acaaacgctt tttggcaatc
    19561 aaagatgctt tcagccatga tgacaacgta ctagaccgct ccatctacga agatagtatg
    19621 ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag tagaagtaaa gcagtatgaa
    19681 ggtcttttag aaacgatgct gaaggagtta gaagaaattt cgccacaaaa gaaaccagat
    19741 ctactggttt atattcgtgt atcctttgaa actatgttag cgagaatcaa aaaaagaggc
    19801 cgggaatatg aacagctaga acaagatcct gaactctatt cctactataa agaactcaat
    19861 cgacgatacg aagagtggta tgaacagttt gatatatgtc ccaaaatagt gatcgatggg
    19921 gacaaatatg atttcgtagc cgatccggca tgtggagaac aaatcgtaca agaaatcaag
    19981 atgcgggcaa agaaaatgac agaagaggca gatgcccagt cgatccgcta cactacaaat
    20041 aacccgaaag caaagggaat ctttacaact aaataatata aaaactaagc aaattgctca
    20101 ctcgcagata tttctctgtg agcagcatct tgcttagttt ttttgattca ttgagatcaa
    20161 atagtgaacc gattcgtatg ataaaattgc cataaatgaa atgtctgagt gagggccatg
    20221 atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc caaacagatg atgacagaaa
    20281 acaaggacaa tagctcctgc gataaaggca agaacgacac ttagatagtg gaaaaatcga
    20341 tcaaaatctg ctttttcttt agaaatacaa taatcatata agttcgttgc tacccctttt
    20401 aaatttccag ttgtaaataa attagtgtag gcacgcccgt tgattttatc aaaagctacc
    20461 cactggatcg atgcagtaaa agcaatcaaa atcgttacta atgcgggatg ctcatggacg
    20521 ggtgtcaaac taacaaagaa aaaaatcact gcttcataat atagaacaaa caagcgccag
    20581 aaatgttttt ctttttttct aaaataatca attagatact ttgttagaag aatccctaag
    20641 aaaaagaaaa gggtagacaa tactttttta ccaacactta gccattctcc gtcaaatgct
    20701 tggatgatcg atagaatgat attgccagtt tgagcagagg caaaagcacc atattggata
    20761 taagtataac tgtccatggc tcctccgata aatgtcagca aaagtcctat tgtacgcccc
    20821 tcgtgaaagg aagtctgcat ttttttcatc accttttatg aatatttttg tcttccttat
    20881 cttagcataa acgattgaaa aaaaggaaaa attttcagaa aactaaaatc atatcgcttc
    20941 ccattttcat ttttagaaag gtctgttagt ataaagaaaa aatagacaac gaaactttaa
    21001 ataaccaact tgaaataaac aaattcaaag aagaaagatg ggaaggaaat caatcagttg
    21061 aatgaaaaag aaattctccg attacaaaaa gaattgaaac gctgggagaa caagtataaa
    21121 aagcttcaac aagtctttcg gctacaagaa gaaatcattg ctacctatga agcggcttat
    21181 ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa aagaaagtga aggaaacagc
    21241 tctttcttta aaatgatgga gaaacgttcc tataatcgct tcaatcaaga acaaaaagca
    21301 gctatcacat atgatatgaa gaaacatctt cgaatcatag caggagctgg gagcggcaaa
    21361 acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc aaaaacaagt agacgaagaa
    21421 cgcatactaa tgatcacttt tacaaggaac gctgcaaatg aactaaaaaa acgagtagat
    21481 aatttcagcc agagaaaaac cgctgtccat atcggaacat tccatagtat cttttttagg
    21541 atatacaatg aaatttgtcg aaaatttcct gaagtagcaa tgcaaggaat acaaggagac
    21601 ttttcaaagg atactgcaca aaaagtgaat gctgttttgc aacagctgat acgtaaatat
    21661 aatctttata tatttgatca atatggagaa aaaacgattg cttctcggtt agactattgg
    21721 cagaatatga acctttcagt agaagaaatg gttcagttag ttaaagaaaa atatgattcc
    21781 attgataaga atgcacgaca gccgatatca gaacgactgt atggattgct cacggaactc
    21841 caagaacaaa aaagaagcca gcaattgcta gagttcaatg atgtactcca aaatctgaaa
    21901 agcgctttgg agaatgagga gatccagcat tatatcggcc aaaaatatga ttatctattc
    21961 attgatgaat ttcaagatac taatccactt caatgggaaa tcgtgaaacg gatgacaaag
    22021 gaaaacagga tcaagttgat tgttgttgga gatgatgatc agagtatcta tggattcaga
    22081 ggatcagagc cagcatacat caagaacttt gaaaaagaat atcctaccaa aactcttttc
    22141 ttattaacta attatcgttc tcgggcagct attgtccaag gagcaaatag attgatcagc
    22201 tataataaaa atgatcggat accaaaggcg atgattccag cacaaaaaga agcaggtgtt
    22261 atggaagcct atttgttttc tgatactaaa gcagaaagtc agtggctaat caaacaaatt
    22321 cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa tcatattata ccgatcagct
    22381 tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga atattccttt tgtactggaa
    22441 gcagatagtc catatgaagg tatctttggt atcaagtctt tccaacattt ctatcaaaaa
    22501 attgttcatt ggcaaacagc actgaatctg caaaagaaaa atcaggccta ccagcaattg
    22561 ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg aatgcgatca atacttttct
    22621 cctaaatatc aaaatatagc cgttgcagat tatattttgt cgataagccc caatttaaaa
    22681 tcgaaatctg cagaaatccg agagtttgag gaagcgttgc gtaaaacaga ccagcatcaa
    22741 gaaatctatt cgcttgttca aagctatctt catctgccac gtatagctaa ggaaatcgac
    22801 cagagtgatc aagaatggat acgagaagaa gtaaagcagc atgctacttt cagaagactc
    22861 caagccttat cgcaagaaac ccaagcgaca gctaaagaat taaaaacccg cttgattgct
    22921 tacagacaag gaaaacttga tgcactatgt atccagagca ttcacaaaag taaaggtctt
    22981 agttaccgaa atgtcttttt gattggttgt aacgaaggaa gtttgcctta taacggagcg
    23041 acagaaaaga aagtaatcga ctttaatgaa ataaaagcag aaccagtaac gacgatagaa
    23101 gaagaacgaa gactatttta tgttgcgatg actagagcaa gaaatcgtct gtatctatgt
    23161 gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga gatttatcaa agaaaccggg
    23221 agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg acagaagtgg tcagtttcaa
    23281 gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt tgtgaaattt cagcttattt
    23341 tcgaagaagc tatttctgga acacagttta ttcggaaaag ggaggtgtga caagaatttt
    23401 gtcacacctc ccttatttat tttgaaaatg agaatactat tatcaaaata gagaatatat
    23461 agaaaaattc tcattattca aacgtaaata atcagttatt ttgatttttt ttatatttta
    23521 ttagctaata attggtattt taaaagaaat tactgtagaa ttggattata attaaaaagg
    23581 agtgagaaaa tgataaaagt atatacttcg cctagctgta cttcctgtag aaaagcaaga
    23641 gcatggttgc aagcgaatca acttgaattt gaagagaaga atatttttgc cgaaccgtta
    23701 acagaaagtg agatcaaaaa gatcttggca gcgactgaag gaggagttca agatattatt
    23761 tccactcggt cgaaaattta cgaaaagctg ggcatagatt ttaacgaatt aactttaaag
    23821 caaatgatcg tcttattcaa agaatatcct tcattattga gacgacctat tttgattgat
    23881 gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc ggtcatttgt ccctcgtgaa
    23941 attcgatgtg ttgccagaaa tctgatctat ttacaaaaca ataacatctt aaattcatag
    24001 ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg cactgtgtaa caagtctgaa
    24061 ggcgaaacaa tggttagaac accatcaatt gccttatcaa tttgttgact tagaagaacg
    24121 cgatttaagt acagaagaag cagaagaatt atgcagtttt gaagaagtgc aagcagaaca
    24181 gctatttgcg acttggtctg aggcttttca caacattgcg attgaccttt cctgtgtagg
    24241 aaaaaaacaa ctgatttttt tatgcacaca acataccaat ctcctcagac gtcctttgat
    24301 tattatcgat gaagttctat ttattggata caatgagcag ttgatggaac agcttatctt
    24361 aaataaatag actttaactg ttttaatcaa gtcactgagc atttcataca gttgtaacag
    24421 tcgaacactt agcatcaagc gtgttatgtg ttcttttttt gactttattt ttctactata
    24481 gagacatgct caatttatgt ggtcaaaatt caaatctagg tggggatata gagttaaaaa
    24541 tatattgaca tgtgtaaatt tacatgatat attcatttct gttgttttaa gtagcaaagc
    24601 aataaaatag tattttaaaa aacatataaa atag
//
LOCUS       DAYYHX010000019        24010 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000019 DAYYHX010000000
VERSION     DAYYHX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.019"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACHA6Y_000575"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACHA6Y_000575"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM8986691.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACHA6Y_000576"
     CDS             complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACHA6Y_000576"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM8986692.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACHA6Y_000577"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACHA6Y_000577"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM8986693.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="ACHA6Y_000578"
     CDS             complement(3374..4681)
                     /locus_tag="ACHA6Y_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM8986694.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="ACHA6Y_000579"
     CDS             complement(4684..5265)
                     /locus_tag="ACHA6Y_000579"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM8986695.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="ACHA6Y_000580"
     CDS             complement(5837..7129)
                     /locus_tag="ACHA6Y_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8986696.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="ACHA6Y_000581"
     CDS             complement(7126..7749)
                     /locus_tag="ACHA6Y_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986697.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="ACHA6Y_000582"
     CDS             complement(7752..8588)
                     /locus_tag="ACHA6Y_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8986698.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="ACHA6Y_000583"
     CDS             complement(8605..9492)
                     /locus_tag="ACHA6Y_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8986699.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="ACHA6Y_000584"
     CDS             complement(9508..10791)
                     /locus_tag="ACHA6Y_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8986700.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11474..12157)
                     /locus_tag="ACHA6Y_000585"
     CDS             complement(11474..12157)
                     /locus_tag="ACHA6Y_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8986701.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12175..12792)
                     /locus_tag="ACHA6Y_000586"
     CDS             complement(12175..12792)
                     /locus_tag="ACHA6Y_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8986702.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12802..13626)
                     /locus_tag="ACHA6Y_000587"
     CDS             complement(12802..13626)
                     /locus_tag="ACHA6Y_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM8986703.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13619..14179)
                     /locus_tag="ACHA6Y_000588"
     CDS             complement(13619..14179)
                     /locus_tag="ACHA6Y_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986704.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14353..15309
                     /locus_tag="ACHA6Y_000589"
     CDS             14353..15309
                     /locus_tag="ACHA6Y_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8986705.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15614..16570)
                     /locus_tag="ACHA6Y_000590"
     CDS             complement(15614..16570)
                     /locus_tag="ACHA6Y_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM8986706.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16674..17276
                     /locus_tag="ACHA6Y_000591"
     CDS             16674..17276
                     /locus_tag="ACHA6Y_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8986707.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17364..18863)
                     /locus_tag="ACHA6Y_000592"
     CDS             complement(17364..18863)
                     /locus_tag="ACHA6Y_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM8986708.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18879..19403)
                     /locus_tag="ACHA6Y_000593"
     CDS             complement(18879..19403)
                     /locus_tag="ACHA6Y_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM8986709.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19701..20450
                     /locus_tag="ACHA6Y_000594"
     CDS             19701..20450
                     /locus_tag="ACHA6Y_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8986710.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20441..21226
                     /locus_tag="ACHA6Y_000595"
     CDS             20441..21226
                     /locus_tag="ACHA6Y_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8986711.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21195..21911
                     /locus_tag="ACHA6Y_000596"
     CDS             21195..21911
                     /locus_tag="ACHA6Y_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8986712.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22013..23032)
                     /locus_tag="ACHA6Y_000597"
     CDS             complement(22013..23032)
                     /locus_tag="ACHA6Y_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM8986713.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23051..>24010)
                     /locus_tag="ACHA6Y_000598"
     CDS             complement(23051..>24010)
                     /locus_tag="ACHA6Y_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM8986714.1"
                     /translation="RKKGIFSESVGKILSKVVLTVALPALAFRAFMQDINTELLMQGI
                     NVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGV
                     MYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLP
                     QVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAA
                     SEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIVIMMATPAATVAAAY
                     AISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7092 a         5186 c         4199 g         7533 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt ttcaatatac tgtattttta tacagaacat atttattaaa
    10861 cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc taggatgaac
    10921 aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg ctaaaaacac
    10981 ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca gacaactgcc
    11041 caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac
    11101 agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg atgcacaagc
    11161 attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc gacaatttag
    11221 ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt ctatagaaag
    11281 cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa aggttttaac
    11341 tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga
    11401 acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt cctcgtcttt
    11461 tgattctcac ctgttattca tgattctttt tctcagtttc ccgatatttt tccattttac
    11521 gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct cctgcttgaa
    11581 tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga agttcaggat
    11641 attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg atattgataa
    11701 tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt accgtactga
    11761 taactggaat atcaactact cgacaaactt cttttactgt ctctaaaggc gtaggaccgt
    11821 tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag
    11881 caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct gcctcaataa
    11941 tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact gcattcgcat
    12001 ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc aattttccaa
    12061 atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata tcttttctca
    12121 attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc tttgctatct
    12181 ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt gcgcataacc
    12241 ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat agttggtggc
    12301 cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga acagttgtgt
    12361 atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt ggatagttat
    12421 ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat ccccataagc
    12481 cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc aatttgattc
    12541 taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt gtagttcttg
    12601 ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat tttcttgctg
    12661 attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta agagtatcac
    12721 tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct ttttccactt
    12781 ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt cttctagcgt
    12841 caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta agtgccaagg
    12901 ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga taaatccata
    12961 ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac ttgcatccaa
    13021 tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg ccagacctgt
    13081 gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg tcgcctcctc
    13141 ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa cttgttgttg
    13201 ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg cttcagcagc
    13261 cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa
    13321 tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg gaccaaccaa
    13381 aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac gatcttttgt
    13441 ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac tagcagttgt
    13501 tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat catcatcgct
    13561 aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc ctaaaaattt
    13621 attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca tgaacgtaag
    13681 tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct tcgatctgtt
    13741 tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat tcatcatatt
    13801 gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc aaagggatca
    13861 gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct
    13921 tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct
    13981 tttgcgttag catccattca taatcagtga aaagattttt tactttctgc atcgcttcag
    14041 atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg atcagcaagt
    14101 attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc ataataagat
    14161 caatatatac ttgatgcacg tttatcccct ccttttaact attctactta agttttgatc
    14221 aaaaagcacc tcttaaatcg ctgctttcat tttttttaat gtttcaagtc ctctcctctt
    14281 tattcttcgt gatttttgtt ataataatag acagacaaga aactcaatcc aactatgaag
    14341 gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg gtcttggtac catcgcacat
    14401 gagtttgccg aaagcttcaa tcaagagacg agcgaactaa cagcagttgc ttccagaact
    14461 tcagaaaaag cggaaaactt cgctcaccgt tacaatattc caaaagctta cggaagctat
    14521 caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag
    14581 catatcgacc atatcttggc agcattggaa gcaggcaaac acgttctatg tgaaaaagcc
    14641 atcacgatga ataaaaaaga actggctgac gctatgcgct tagcagaaga aaagaatttg
    14701 atcttagcag aagcgatgac aatcttcaac atgccacttt atcagcagtt acgctcaata
    14761 atggatacag gaaaactggg ggctttaaaa atgatccaag cacctttcgg cagttacaaa
    14821 gaaccagacc ctaaaaaccg gttcttcaat ccagaattag ctggaggagc tctgttggat
    14881 atcggtacgt atgctgtatc tttcgctcgt ttctttttga gctctcaacc tgaagtagtt
    14941 gcctctacaa tggttccttt tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt
    15001 aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc
    15061 ggtatcgttg cctttgagaa cggttatgtg acgattgctg attacccgcg tgctgatcgt
    15121 gcggagatct tatttacaga tggtacaaag gaattcatcg aaagcgggaa tacatctgaa
    15181 gcaatgaatt acgaaataaa gaatatggta cacatgattg aaggtgactt gcccaatcgc
    15241 tctctcttct tgacaaaaga tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa
    15301 aatatgtaat tttaaaaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
    15361 aacaaaaata gctcctccta tttttcgttt ggctggactt attaccgcag aggaaagctt
    15421 ttgaacaccg tttaagaaag aggttgtgag cctaccgttc agctccgagt cacaaggagc
    15481 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttga
    15541 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
    15601 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
    15661 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
    15721 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
    15781 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
    15841 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
    15901 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
    15961 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
    16021 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
    16081 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
    16141 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
    16201 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
    16261 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
    16321 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
    16381 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
    16441 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
    16501 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
    16561 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
    16621 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
    16681 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
    16741 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
    16801 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
    16861 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
    16921 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
    16981 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
    17041 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
    17101 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
    17161 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
    17221 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
    17281 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
    17341 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
    17401 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
    17461 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
    17521 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
    17581 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
    17641 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
    17701 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
    17761 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
    17821 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
    17881 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
    17941 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
    18001 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
    18061 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
    18121 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
    18181 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
    18241 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
    18301 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
    18361 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
    18421 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
    18481 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
    18541 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
    18601 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
    18661 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
    18721 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
    18781 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
    18841 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
    18901 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
    18961 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
    19021 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
    19081 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
    19141 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
    19201 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
    19261 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
    19321 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
    19381 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
    19441 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
    19501 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
    19561 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
    19621 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
    19681 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
    19741 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
    19801 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
    19861 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
    19921 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
    19981 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
    20041 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
    20101 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
    20161 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
    20221 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
    20281 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
    20341 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
    20401 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
    20461 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
    20521 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
    20581 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
    20641 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
    20701 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
    20761 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
    20821 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
    20881 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
    20941 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
    21001 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
    21061 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
    21121 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
    21181 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
    21241 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
    21301 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
    21361 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
    21421 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
    21481 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
    21541 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
    21601 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
    21661 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
    21721 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
    21781 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
    21841 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
    21901 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
    21961 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
    22021 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
    22081 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
    22141 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
    22201 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
    22261 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
    22321 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
    22381 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
    22441 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
    22501 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
    22561 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
    22621 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
    22681 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
    22741 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
    22801 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
    22861 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
    22921 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
    22981 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
    23041 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
    23101 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
    23161 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
    23221 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
    23281 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
    23341 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
    23401 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga aatagccacg
    23461 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
    23521 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
    23581 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
    23641 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
    23701 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
    23761 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
    23821 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
    23881 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
    23941 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
    24001 ccttttttct
//
LOCUS       DAYYHX010000020        23830 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000020 DAYYHX010000000
VERSION     DAYYHX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.020"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACHA6Y_000599"
     CDS             complement(54..788)
                     /locus_tag="ACHA6Y_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8986715.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACHA6Y_000600"
     CDS             1115..1525
                     /locus_tag="ACHA6Y_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM8986716.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="ACHA6Y_000601"
     CDS             1518..2219
                     /locus_tag="ACHA6Y_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM8986717.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ACHA6Y_000602"
     CDS             2219..3805
                     /locus_tag="ACHA6Y_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8986718.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ACHA6Y_000603"
     CDS             3824..4045
                     /locus_tag="ACHA6Y_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM8986719.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ACHA6Y_000604"
     CDS             4042..4803
                     /locus_tag="ACHA6Y_000604"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM8986720.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ACHA6Y_000605"
     CDS             5183..5692
                     /locus_tag="ACHA6Y_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8986721.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ACHA6Y_000606"
     CDS             complement(5767..6306)
                     /locus_tag="ACHA6Y_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM8986722.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACHA6Y_000607"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACHA6Y_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM8986723.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="ACHA6Y_000608"
     CDS             7319..8170
                     /locus_tag="ACHA6Y_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM8986724.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="ACHA6Y_000609"
     CDS             8198..8833
                     /locus_tag="ACHA6Y_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM8986725.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="ACHA6Y_000610"
     CDS             8853..9626
                     /locus_tag="ACHA6Y_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8986726.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="ACHA6Y_000611"
     CDS             10034..10831
                     /locus_tag="ACHA6Y_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM8986727.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="ACHA6Y_000612"
     CDS             10809..11222
                     /locus_tag="ACHA6Y_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8986728.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="ACHA6Y_000613"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="ACHA6Y_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8986729.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="ACHA6Y_000614"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="ACHA6Y_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM8986730.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="ACHA6Y_000615"
     CDS             15999..16559
                     /locus_tag="ACHA6Y_000615"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM8986731.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACHA6Y_000616"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACHA6Y_000616"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM8986732.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="ACHA6Y_000617"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="ACHA6Y_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM8986733.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="ACHA6Y_000618"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="ACHA6Y_000618"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM8986734.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="ACHA6Y_000619"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="ACHA6Y_000619"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM8986735.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="ACHA6Y_000620"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="ACHA6Y_000620"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM8986736.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="ACHA6Y_000621"
     CDS             21906..22484
                     /locus_tag="ACHA6Y_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8986737.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="ACHA6Y_000622"
     CDS             22536..23018
                     /locus_tag="ACHA6Y_000622"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM8986738.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="ACHA6Y_000623"
     CDS             23135..23509
                     /locus_tag="ACHA6Y_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986739.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4286 c         5115 g         6648 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgac
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatggaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaaca atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt
//
LOCUS       DAYYHX010000021        23618 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000021 DAYYHX010000000
VERSION     DAYYHX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.021"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            257..2023
                     /locus_tag="ACHA6Y_000624"
     CDS             257..2023
                     /locus_tag="ACHA6Y_000624"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM8986740.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2125..3384
                     /locus_tag="ACHA6Y_000625"
     CDS             2125..3384
                     /locus_tag="ACHA6Y_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8986741.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFQKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3531..4832
                     /locus_tag="ACHA6Y_000626"
     CDS             3531..4832
                     /locus_tag="ACHA6Y_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8986742.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4835..5686
                     /locus_tag="ACHA6Y_000627"
     CDS             4835..5686
                     /locus_tag="ACHA6Y_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8986743.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5699..6514
                     /locus_tag="ACHA6Y_000628"
     CDS             5699..6514
                     /locus_tag="ACHA6Y_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM8986744.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6665..7831
                     /locus_tag="ACHA6Y_000629"
     CDS             6665..7831
                     /locus_tag="ACHA6Y_000629"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8986745.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8061..8708
                     /locus_tag="ACHA6Y_000630"
     CDS             8061..8708
                     /locus_tag="ACHA6Y_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8986746.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8792..9736
                     /locus_tag="ACHA6Y_000631"
     CDS             8792..9736
                     /locus_tag="ACHA6Y_000631"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM8986747.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9871..11322
                     /locus_tag="ACHA6Y_000632"
     CDS             9871..11322
                     /locus_tag="ACHA6Y_000632"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM8986748.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11343..12179
                     /locus_tag="ACHA6Y_000633"
     CDS             11343..12179
                     /locus_tag="ACHA6Y_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8986749.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12179..12838
                     /locus_tag="ACHA6Y_000634"
     CDS             12179..12838
                     /locus_tag="ACHA6Y_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8986750.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12964..13653
                     /locus_tag="ACHA6Y_000635"
     CDS             12964..13653
                     /locus_tag="ACHA6Y_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986751.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13659..14615
                     /locus_tag="ACHA6Y_000636"
     CDS             13659..14615
                     /locus_tag="ACHA6Y_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM8986752.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14659..15171
                     /locus_tag="ACHA6Y_000637"
     CDS             14659..15171
                     /locus_tag="ACHA6Y_000637"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8986753.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15427..15975)
                     /locus_tag="ACHA6Y_000638"
     CDS             complement(15427..15975)
                     /locus_tag="ACHA6Y_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8986754.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16064..17245)
                     /locus_tag="ACHA6Y_000639"
     CDS             complement(16064..17245)
                     /locus_tag="ACHA6Y_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM8986755.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17481..18989)
                     /locus_tag="ACHA6Y_000640"
     CDS             complement(17481..18989)
                     /locus_tag="ACHA6Y_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM8986756.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19148..19339
                     /locus_tag="ACHA6Y_000641"
     CDS             19148..19339
                     /locus_tag="ACHA6Y_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986757.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19542..20459
                     /locus_tag="ACHA6Y_000642"
     CDS             19542..20459
                     /locus_tag="ACHA6Y_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8986758.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20452..21525
                     /locus_tag="ACHA6Y_000643"
     CDS             20452..21525
                     /locus_tag="ACHA6Y_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986759.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21673..22191
                     /locus_tag="ACHA6Y_000644"
     CDS             21673..22191
                     /locus_tag="ACHA6Y_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986760.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22172..22564
                     /locus_tag="ACHA6Y_000645"
     CDS             22172..22564
                     /locus_tag="ACHA6Y_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM8986761.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22640..23575
                     /locus_tag="ACHA6Y_000646"
     CDS             22640..23575
                     /locus_tag="ACHA6Y_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8986762.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
BASE COUNT         7787 a         4188 c         4721 g         6922 t
ORIGIN      
        1 ctacgccaaa aactgttaaa aaacttttac tttagaaaat tggagaaatt gatcgcttaa
       61 gataaattca ggacactaaa tagtacagga agtagactaa aacttttaaa agttttaagt
      121 ctatctattt ggcaagttta aaaaataaaa attcggtatc aaaaaactgt taccgataac
      181 atttttgaaa gcgttgtctt ttgcttctct atcacttata gtgatgacgt catattaaaa
      241 cacaaggagg aaatcaatgg atacagctgc aatttatcat cgaccggaaa gtgaatatgc
      301 atttctatat acgaatacaa gatttcgtat tcggcttcgt actaggaaaa atgatatcaa
      361 aaaagtatat gtgctatgtg gagatccata taccataaca actgaaaaat ggtatcaaaa
      421 acaacgacca atgaaaaaat gtctaagtac caatgttcat gattattggg aaattgaagt
      481 gacgagcgaa acaagtcgct tacagtatgc gttccatgtt gttggggaag ataacacgga
      541 ctgtttttac ggtgatcaag gaatttttcc ttatcgagaa aatgtgatta ctgaaccaaa
      601 tttttatttt cgaatacctt attttcatca aatcgatcga ttcactattc cagaatgggt
      661 aaaagaaaca gtatggtatc aaatttttcc cgaaagattt gcaaacgggg acaaagcaaa
      721 tgaccccgaa cacactttac cttggggaag taaagatcca gaccgagaag actttttcgg
      781 gggagatttg caaggtgtga tggatcattt ggattacttg acggaccttg gcatcaatgg
      841 catctatttt tgtcccatat ttaaagccac atccaatcat aaatacgata cgattgatta
      901 ttatgaggtg gatcccgctt ttggcgataa aaaactttta aaaaacttga ttgatgaagc
      961 acataaaaga gggatacgta tcatgttcga tgctgtattc aatcatatgg gtgtccattc
     1021 accacagtgg caagacgttc tcaaaaacgg agaaaaatcg atctataaag actggttcca
     1081 catccgtttt ttcccagtag attcttatca gatgactgac ttaccagaaa ctgctgaaaa
     1141 tatcccatat gatacttttg cttttactcc attcatgccg aaactaaata cagcaaatcc
     1201 agaagtgcaa aaatatttgc tggatatcgc aacttattgg ataaaagaat tcgatattga
     1261 tggatggcgt cttgatgttg ctaatgaagt cgatcatcat ttttggaaaa aattcagaga
     1321 agcagttaca gaaatcaaac cagacatcta tatccttgga gagatttggc attcctctca
     1381 agcttggctg caaggggatg aattccatgc agtgatgaat tatgcattta cagattcgat
     1441 taaagattat tttgcaaaaa agaaaatcac agcttcacaa atggtcagtg gaatgaatca
     1501 tcagcaaatg ctttacagag atcaggtgaa cgaaggaaca ttcaacttat tagattctca
     1561 tgatactgcc aggatcttga ccctttgcca aggtaacaaa gaattgatga agtctgtgat
     1621 ggcctttatg tttcttcaaa aagggtctcc ttgtatctat tacgggacag aaatagggat
     1681 gactggtgaa gatgacccgg attgccgcaa atgtatgata tgggagaagg aggaacaaga
     1741 tctagagcta ttcggtttta taaaagaact tgtttctcta aggaaacagt tgtctaagat
     1801 tatttctgaa ggttctactc agtggctaat agtaaatgat cgtgaagata agctctattt
     1861 cacacggaaa ctagagggac agatcatcta tgtttacttt aatcagtcaa aagaaccttg
     1921 ggtagtagaa caggaaaatg aggtgattct tagtcaaaat tgtcagttgt tagaagacgg
     1981 gaaagcagag atacgacaat acggattttt gatcatgtgt tgaaaactgt taccggtaac
     2041 aacaagaaag cgtttgcaaa tgaagatttg tgcccattat aataagggtg taacagcacg
     2101 ttgaatacaa aggaggaaat gaagatgaaa aaaagaagtg tgaaacagtt gggtttaggg
     2161 ttaatctctt taggaatctt aggcggatta gcggcttgtg gcaatggtaa ctcttcagca
     2221 gaaggggaag ccgatgaagg aaaaacgtta acagtttccg ttgattctgg atataaagat
     2281 tatataaacg aaataaaaaa cactttccaa aaagaaaacg atgtgaaaat caagctagta
     2341 gaaaaggaca tgttcgatca gttggaatca ttagcgttag atgggcctgc tggaaaggga
     2401 cctgacgtta tgatggcagc atatgaccgg atcggtgcgt taggacagca aggacatctg
     2461 gcggaagtaa aactgggaaa tgaagcagct tacgatgaaa cagacaaagc ccaagtgact
     2521 tatgatggaa aaatctacgg agaacctgca gtaatcgaga cccttgtttt gtactataac
     2581 aaagatttag tcgatacagc accatcaacg tttaaagatt tggagaatct atccaaagac
     2641 agtcgttttg cttttgagtc agaagcagga aaaaatacag gatttttagc taaatggact
     2701 gacttttatt attcctatgg tttaattgca ggttacggtg gctatgtatt tggtgatgat
     2761 ggcacaaatc cttcagatat cggtttgaat aatgcaggtg ctgtagaagg aatctcttat
     2821 gcaacagatt ggttcaaaaa tgtttggcca aagggaatgc aagacataaa aagtgcagga
     2881 gacttcgcca gccagcaatt catgtccaat aaaactgcag caatcattga cggtccatgg
     2941 caagcgcaaa catacaaaga aaacaatatc aattatggtg tagccaaaat tcctactcta
     3001 aataatggac agccttatca accgtttggc ggaggaaaag gatgggtagt cagcaattat
     3061 gctaaaaaca aagacctctc tcaaaaatgg cttgattatg taacgaatca ggaaaatcaa
     3121 gaaaaattct ttgaaatggt aaatgaaatc cctgcaaacc aacaagctag ggaaacagca
     3181 aaggctaaaa acgatgaatt aacaacagct gtgattgaac aatatgaaac agctcaagcg
     3241 atgccaaaca tcccagaaat gggcgaagtt tggacaggtg cagaaaactt gatgttcgat
     3301 gcagcatcag gttcaaaaac accaaaagaa tccgcagatg aagcagtaaa aacaatcagt
     3361 gaagccatag aacaaaaata ctgattaaaa tgataaccgt ccatttttat aaaaaatgga
     3421 acaggtgctt cagataaaag tggcaagagc gagatggaaa attatcttgc tcttgttgcc
     3481 attatcttaa aagcagtttg cggtttagca agtttagagg gggcattcag atgcagcaag
     3541 ataaaacaag caatatcaac gaaaaaaaaa tcacgctttg ttcacttatt ccagggatgg
     3601 ggcagtttgt aaatggccaa gcttttaaag gtatcgtttt tttcgccata ttcgtcctat
     3661 tcattggtca gatgcttctg ggcggatttc atgcattggt aggattggtt actttgggaa
     3721 gtataccaat ggaagatcat tcattattta ttttgattga aggcactttg caattcatca
     3781 ttacaatcat tttcttgatt ttttacggtt tgaatctttt agacgcttat cgagtagcga
     3841 agatgcgtca agagggcaaa aaaatcaatc ggacgatgaa ggaagtcata cataatatag
     3901 gtgatgaagg ttttccttat ctattgacac ttcccgctta tcttctcatg atttttacga
     3961 tcattttccc agtcctagtt acattgttca ctgcatttac taattatgac ttcaatcata
     4021 ttccaccagc cagtttaatt gattgggtag gggctaaaaa cttttttagt atcttctttt
     4081 taagctcata tcgtaatact ttttttgcag tcttttcgtg gaccgtgatt tggactttat
     4141 gtgcaacaac tttgcaaatc acccttggta ttctcacagc ggttgttgct caccagaaat
     4201 ttattaaatt caaacgtatt ttcggtatca ttttcttatt accatgggcg gtcccagctt
     4261 ttattactat catgagtttt tcgaatatat tcaacgatag cgtaggtgcc atcaatactc
     4321 aagtgattcc tttcataaac cagcttcctt ttgtagatat cagtgcaatc tcttggaaaa
     4381 cggacccgaa ctggacgaaa gtagcaatta tcttagtgca gggctggtta ggatttccct
     4441 atatctatgt gatggttaca ggaatcttac aatcgatttc agaagactta tatgaagcag
     4501 caaaaatcga cggagcaaat gcatggcagc gcttttcaaa catcacgctt cctgctgtct
     4561 tcctagtggc agcgccaaca tttattacac agtatacggg gaatttcaat aatttctcca
     4621 tgatctatct gtttaacaat ggcggtcccg gaagcgtagg cggaggagct ggttctactg
     4681 atattcttat ttcctggatc tataaactta cgactggaac ttctccgcag tattctatgg
     4741 cttcagcaat cacgttgatt atctccgtca tcgttatctg tgtgtcattg ctggtattta
     4801 aaaagacaaa tgcgttcaat atgggggatt aacaatgaaa aaatttcatt cgagtgctcg
     4861 attccaaaaa cgactttctc acttttttac ttactttttt cttttaatac tgtcagtcat
     4921 tatcatctat ccgcttttga tcacagcaag tactgccttc aagtcaggaa atattgcagc
     4981 ttttagttta gatctttcaa gtgactggac tttaggaaac tttactcggc tattcagtga
     5041 aacactttac gggacttggt ataaaaatac attgatcatt tctgtcctaa caatgatcgt
     5101 acaagtgact gtagtaactt tggcgggcta tacttatagt cgctatcgtt ttatcggccg
     5161 caaatcaagt ttgatgttct ttttagtgat tcagatggta ccgactatgg cagcattgac
     5221 tgccttttat gtcatggctt tcttattaga agcgctcgat aagtactggt tcttgacaat
     5281 gatctatata ggcggtggaa ttccaatgaa tacttggctg atgaaaggat actttgacac
     5341 tgtaccaata gatttagatg aatctgctaa acttgatggg gcagggcatt tcaggatttt
     5401 tttccagatt gtattaccgc ttgtcaaacc gatgctcgcc gttcaagcat tatgggcttt
     5461 tatgggacct tttggggatt tcatgttagc taagttcctg cttcgaacac ctgaaaatca
     5521 gactgttgct gtcggcttac aaacatttat ttctgatgtg acaaaccaaa gagtctctct
     5581 ttttgctgct ggggcaattt tgattgcact accgatttgt ctattattct tctttttaca
     5641 aaagaacttt gttaccggcc tgacatctgg cggaacaaaa ggataggagc agagtatcat
     5701 gcaaacattt ccgttaaact atttttccgc attacgtacg ccaactcaag tgtttgcggg
     5761 acgcaaacta ttatcatggc ctaaattttt cttgatattt gtatttttag tttccttgat
     5821 ggtcatgcct gtgactcttt tttatgccaa tcagatccaa gcgattccat tagaacaatt
     5881 tctatccgtt cattcgctga tcgatgaaca aggaacccaa aaattttctg aacttgaatt
     5941 atctgaaact gggttacaag catcgcaaca aacgatagct gtgacccctg aaattttggt
     6001 aggtgtgtct ttatctgaga aacaacaatc cgatcatgga acttttattg attttgagaa
     6061 ggaacaatgg gtgatccaac aaaaggataa atctggtata cgccgctata cgatgaatta
     6121 tagcccatct ttccaaccag attctgtacg tacgcccgaa gatttccaac gatttttaga
     6181 aagagaattt tatgccagta atcgtccaac aatcatttta tcttatagtt taagtcttgg
     6241 tcttgtccta tttgtcatga cgagtctcat tttattcggg gcttcctttt tcttgtggat
     6301 gacaagaaaa agccaacttt catccattca tacattcaaa gagagtgcaa atttgatgct
     6361 gaatgttatg ggaataggtt ccatggcagc agcagttgta gggtttatcc actttgactt
     6421 tatcttgatg ctaagtgtcc aaacaacgat aactgtcttg ctgcttttat gggtttttgt
     6481 caaaacaaga ttcaaagata agcgtgtcga atgacaagtt taagcgattt atgcgacaat
     6541 gaatgtgaaa aaaatgaaaa ataaagtagt ttttgaaaaa tttctgaaaa aattaaactt
     6601 cttagttgat tcttttcttt tttagtatta acgtaatagg gtagtttttt taaggaggaa
     6661 aatcatgtta gagaaaactt tatacaatca attattttca cgttctttct cgctccctgt
     6721 cgaagtaaat tattgggacg gtacaacgaa acaatacgga gatacggaca ctcctcccca
     6781 aatcaaaatc aatatccatg aagaaatccc aatgaaagag attacaagta acgcttcttt
     6841 ggcactagga gaagcctata tggatcatcg aatcgagatc gagggaagta tccaagcatt
     6901 gatcaacgat gcttatcagc aagcaaacag ctttttacga ggaaaagatt atctaaaatg
     6961 gctgccaaag aaagaaaaac atacgaaaca aaaaaacaaa gaagatatcc atgcgcatta
     7021 tgacttaggt aacgactttt ataaactttg gctagatcca actttgactt attcttgtgc
     7081 ttatttcaat acacctgaag atacgttgga acaagcacaa atcaataaag tccatcatat
     7141 cttagacaaa ctctttatca aagaaggcga aacgttgctt gatatcggtt gtggctgggg
     7201 aacattgatg ttcactgcta ccaaagagta taatgtcaag gcaactggga tcacactaag
     7261 tgaagaacaa tacgatttga ttaccaaaag aataaaagaa gaacatttag aagataaatg
     7321 ccgtgtatta ttaatggatt accgcgaact aaaaggcgaa acgtttgatc atatcacaag
     7381 tgtaggtatg ttcgaacatg taggttctga aaatcttgaa ggttatttca aagtggtaaa
     7441 agatttattg aaaccaaaag gaacagcttt gatccatgga atcagccgac aacaaggcgg
     7501 tgcgacaaat gcttggatca ataaatatat tttccctggc ggttatgtcc ctggtgttac
     7561 tgaattagtt gataatatta caaaaaacga tttgcaactg attgatttgg aaagcttacg
     7621 tcgtgactac caattgacat tagaacattg gacaaagaat tttcatcaag tccaagatca
     7681 agtcacgaaa gaaatgggtg attccttcta tcgaatgtgg gatctatatt tacaagcttg
     7741 tgcagcttca tttgaatcaa gtaatatcga tgtgattcaa tatttgcttg ttcaacctgg
     7801 aaacaatgac atcccaatgc atcgtgcata attgaggcaa aagtactgtt taaagactat
     7861 ttaaaaagaa gctaagtttt atgatgctcc agaagtgaac ttttttgtct aacttctagg
     7921 attgtctcta aagcttagtt tctttttctt tatattttca taataaaagt ttggttcttt
     7981 aatttttaaa catgacagaa aattttttct tcgttatact tgtaatagaa tactatttta
     8041 taacaaaggg gatcgaatca atgaaaaaaa ctgttattta tactagttta tttgcagctt
     8101 tgtttttagc tgcttgtcaa tcaaatacca atcaaggcac atctgggaat acgtcttcca
     8161 gtagcgaaca gcgctcttca caggtatcgg actcctcaac aaaagcaagc acatcttcgt
     8221 ctagccagac aaaaagttct agtgaagcaa gtactcagtc agatatcaaa gtgtctgcag
     8281 ctcgtgcgat cgaattgttc caagataaat acccagatac tgcaattact agtttggaat
     8341 tagatagtga ttggggatct tacttctaca agatagaagg tgtcgatgat caaaaagaat
     8401 acgaagcaag aattgatgct caaaatgaaa aaatggagac agagaatcca gaaactctag
     8461 atcgagatga acaaaatggt caaaagaaaa aagaagatgg tctggatgtt gataaattga
     8521 tcacgattga ggaagcagca aaaatcgccg tcgacaaagt aggaagcggt acggcaacag
     8581 attgggattt ggataaagat ttaggtacta cttattggga agtcaaagta aaagacggca
     8641 atcaaagtac atcagtcaag atcaatagcc aaacaggcga agtactgtca actgaacaag
     8701 atgattaaga atttcttcgg aaaacaagag ttgatttttc ttttagtcat gatacactag
     8761 acaaaataac ttgtttgaag gagcgattta cttgagttta gaaacgacct ttgccaatca
     8821 tatctttgcc aatcctttga tgaatgcatc tggtgtccac tgcatgacta cccaggaatt
     8881 agatgaactg gctcattcag aagcaggagc atttatcact aagagctgta cgatcaatga
     8941 acgaaaagga aatcccgaac cacgctattt tgatgttcct ttagggagta tcaattctat
     9001 gggacttcca aacttaggtt tttcttatta tctagagtat gctcttgcgt atgaaaaagt
     9061 gcaggaaaac caaaatcaac cgttattttt ttccattgcc gggatgagtg tccaggaaaa
     9121 cttggaaatg cttgagaaaa tagaaaaaag tgggtttaat gggatcactg aattaaattt
     9181 atcttgtccc aatgtgcctg gaaaaccaca actagcttac gattttgagg ccacttatga
     9241 aacactgaaa gaagtattca gcatcttttc taagccgcta ggaatcaaat tgcctcctta
     9301 ttttgatttt gctcattttg atcagatggc tgatatcttg aatcagtttc cattgaccta
     9361 tgtcaatgct atcaacagtg tgggcaatgg tttgtatatc gatacagaac aagaagcagt
     9421 agtcatcaaa ccaaaagaag ggttcggcgg cattggcgga gagtatatca agccgactgc
     9481 tttggcgaat gttcgcgctt tttatacacg tttgaaacca gaaatccaaa tcattggtac
     9541 aggtgggatt cggacaggac aagatgcatt tgaacatctg ctatgtggcg caagcatgtt
     9601 acagattggt accgagcttc ataaagaagg gccggaaatt ttttctagaa tcataaaaga
     9661 attaacgcag attatgtcag aaaaaggata tacgtcaatt gacgaattta aaggcaaatt
     9721 acgcaccatt tcatagcaaa gagacgaagg tcatgattga tcttcgtctt tttttataaa
     9781 aaacactcgt ctgttcattc ctgtgcaagc gtactcattg tatgttagac gaacttgtgg
     9841 gaaaataaca gaggcaaaaa ggaggataat atgaatgatg caacagaatt agcaaaagag
     9901 cttaaagaaa aagaatttag ttttcaagaa ctagttacgg atatacagaa aaaaataaag
     9961 aagcaaaatc atgaattgaa tgcgtttgtc gcgcttgaac cattcgagag taattcccac
    10021 gagctattga ttccccagtc agaaaaaggg ccattggctg gtatcccatt tcctttaaaa
    10081 atgctcggtc aagaaaaaaa gggatggcta gctacttcag gatctcgttt atttgaaaca
    10141 caccgagctt ctcatacatc taattatgtg cggcaagcag aagcgatagg tctagttcct
    10201 tttggacaaa ccaatgcgcc agagtttggt ttcaaaaata ttacagaccc agtcatctat
    10261 ggcccagcaa gaaacccttg gaaccttgaa tgtacaccgg gcggatcaag tggaggagct
    10321 gctgctgcag tttcatcagg aatcgttcct ttagctggtg ctagtgatgg cggtggttct
    10381 attagaatac ccgcttcgtt ttgcggattg attgggctga aaccttcgcg agggacgatg
    10441 ccagttggtc catatgcttg gagaggatgg caaggagctg ctattgattt cggcttgaca
    10501 gtttctatga gagacacgga ggctttattt tatgggatga gaagtatcca ttccggtgcg
    10561 ccatatcaag tatcgccagt tgagtggcaa acacatccaa gaaaaaaacg gttgaagata
    10621 gctttttgtg ttgattcacc gatccactca cagattagtg aagaggcagt acttgctgta
    10681 aaaaaagctg tagatttcct tggaaaagaa gggcatgatt tgacagagat tccttaccca
    10741 ctagatggtc gtaaactgat cacgtcttat tatcagatga atggcgcaga aacagcagca
    10801 atgatccgct cgattgaaac aggcatccag cgagctgtca gaaaagaaga acttgaaccc
    10861 atttcttgga ctttgttcca atatggtcag aagctctctg ccgctactta catcgattct
    10921 ctgcatgtat gggatgaagc agcaattgtc atggaaaacc tacatcaaga atacgatcta
    10981 ttcctttcac caacgactgc atttaccgcc cctaaaatca gcgaagattt gcaaagtgat
    11041 gcgataagag cacagatgga acacatcagt gattgttctg aagtagaagg tttagacgtt
    11101 gtttcttcca tgtttgagga gagcttgaag atcacacctt atacacagtt ggctaatctg
    11161 actggacaac cagcaatcag cttgcctact catttgacaa aagaaaacat gcctctagga
    11221 atccaattta tggctgccag aggaagagaa gatttgctat ttcaaatcgg ctatctattt
    11281 gaagcaaatc atcagttcca tttaccaaat ccatggaaat agcagaaaca aggagaagaa
    11341 gaatgaagaa aaaaagattg tttgctctaa taactagtgc cttttttatt ttaagcgctt
    11401 gcggtacagc tgccgctaat caggaagata acagctggag taatgtagaa aaaaagaaag
    11461 aattagtagt agcgacttct ggcacactgt ttcccgcatc ttattataat gatcaaaatg
    11521 aattagttgg ttatgatgta gatttggcaa aagaggtggc taaacggtta aatgtcaaaa
    11581 tctcctttaa agaatacaac gtggatggac aaatcacttc tgtttcaaaa agagaatcag
    11641 attttgctgc gaacgatttt ggtatcacaa aagaaagaga aaaaaaattc tccctttcag
    11701 agactttcaa atattcattt gacagtatga tcgtacgaaa aaaagatgat tcaggaatcc
    11761 actcacttga agatcttaaa gggaaaaaag cagctggcga acccaatact agctatatgc
    11821 gtttagcgga aaaacacgga gctaaattag tcacatatga caatgccacg aatgatcagt
    11881 atttaagcga tgtagcaaat ggtcgaacag atgttattct aaatgattat tatctgcaga
    11941 aaatggccgt atctgcttta ccagatatcc ctgtaaaaat actggagaac gtttatttca
    12001 atgaaaacga aagtggtttt ttattgaaag aaggaaatga tgctctgaaa aagaaaatcg
    12061 atggcgtatt aaaagagatg aaagaagatg ggacgacaaa gaaaatatct gaaaaatatt
    12121 ttcaaactga tgtttcagtc aaaccgaaag aaacgatggc tgagtcggtg agtgagtgat
    12181 gtactttcct ctaattgatt tttccctcat gtggacttca cttcctttcg tactgcaagg
    12241 cttggtatac acattaggga ttggcttcgt gtcgtttctt ttaggaaatc tggtagggtt
    12301 gcttttgaca gttttaggtc ttttggattg gctgcctctg aatgtattta ttcgttttta
    12361 tttatcgttc tttagaggca ttcccgcact agtcctgctg tttctgttat attttgggtt
    12421 accttatcaa ttaagtgcct taacggcttc agtcatctgc tttacgatta ccagcagtgc
    12481 atttattggt gaaatctatc gaggatcact agcaggcgtt tcttcaggtc agtgggacgc
    12541 tggtttagct tctggttttt ctttctttgg ggtattgaaa tatatcgttt tgccgcaagc
    12601 ttttcgaatc tcagtaccgg cactcagtaa cgtagctatg gatctagtga aaggtacttc
    12661 gttagcagca acaatcacag tgccagaaat atttcaaaaa gcaaagatca taggcggtcg
    12721 gacattcgat tatttttctc tctatatttt agttgcactg atttattggg tcatttgtat
    12781 cgggatcgaa aaagtgcaac agtatttaga aaaacgttct atgcatcagt caatctgaaa
    12841 aatagaaatg gtgctagcgt ggctggcacc atttctattt tttgtttctt gtatattctg
    12901 cctagtattt taaaaccatt tcatgtaaaa tgtaaatcga aaatatgttc ggaggtagaa
    12961 aacatgaaga ccccgctaac catcgaaata gaatcctcgt tataccaata ttgcatcgag
    13021 caaggcggct tggttgtaga agaagtcacg atgcctgatg accaaggaat tgtagatacg
    13081 cttagctgct tgacaaaatt tgatggaagc cgagaatggc gatgctatga gctgaaagtg
    13141 acaaaagcag attttcgctc tacagccaaa ctgtcatttg ttggacatta taattatttt
    13201 gttttaccaa gtaaactata tgaagaagta aaaactgaaa tccctacagg aataggtgtt
    13261 ttagtgtatc gtccgtacac ggttgaagag gagatgcctg cctctggtac atttctgatt
    13321 gcgaaaaaag cagcaaagaa agagcttgct gtaccggaag aagctttgac aaaccgcttc
    13381 atggcttctt tattcaggga agtaagaaaa gcaaagcaaa tggagcgagg cgtaaaatat
    13441 tttccaacag atcaattgta caaagaattg aaaagacgtt cagaaaatca agatccattt
    13501 tctaacgaac gatattatga acggttttta gaggaaatag ctagtcaggc aatcaatgag
    13561 ttgcaagaag aagttatcgc gcttcagcat gattatgaat atttgaggca acaacagcag
    13621 atccgtcggt tgcctacaga gccattggag tgaaaaatat gtactatcct tatttaagag
    13681 gaaaacaatt tgacctatta gcattaaaag aagcactgtc gcgaggattg ctctccaata
    13741 aaatccaacc agtgatcgaa ccagtacgtg attccgctac gcttaaaaat gtgatcgaat
    13801 tatttcaaaa aaagcaccat ccaatcgctg tgatccaaaa tccccaagta ggacaattca
    13861 aattattcga tcagcatgtg cacagttgga cagtcaaaga aaatagctct gtcgtccctg
    13921 cacagatctt aacgccagaa aatctcagtg aagtactgga ttcacctcca gcgtttcttg
    13981 ttttcgatgg acagcactac ccaaaagata cagaggtgtg gaaacagttg gcaggagttg
    14041 attcgaaatt tttgatacca gatactagtc gctttcgcat ctggctgccc gaaaacaaaa
    14101 ttgtgatccg tgactctttt caaacgagaa agcatgttga aagttatgca gataaaacag
    14161 atgacttttt tagtgatgac tatctttttt tccacgcaga cggatatatt ggattttctg
    14221 atttcacgat cgaaggtagc cgttatttcg ataaagggtt ccctagccga gcgcttgcta
    14281 tccatctaac gtatattgat gcttacggca atatacgcgt gaaacatttt gtctctgaca
    14341 gtaacgactc tgctaaagac caagcattga agtttcttga agcaggagat aaaatgaaaa
    14401 aatggatcat gcgtcaccat catcaactct tgattacttc aggaatgatt gaattattgg
    14461 ctctttacca gcaacaaaaa ttccctggac tgggagtgtt gaaaaagtgg tcacttatgc
    14521 atcatttgga acttattagt cacctgctag atcatccaaa agattggctt aaatattatt
    14581 caaaagttca ggaactatat gaagtgatcc aatgattaga taatgtaaat gaaaaaggaa
    14641 tagaaaagag gaaactggat gtatattcgg caaacaagga aagaagaagt agaagaagtg
    14701 gtagaaatca ttaatgccgc agcggatttc attgcttcgc aaggctctcc tcaatggcaa
    14761 aacgggcaga aaccaacaaa agaaacgatt ttgaacgata ttgaaagaaa agaaagctat
    14821 gtattaatag tggaagatca aattgctgga acagctgcat tagtctcaag cgtggatccc
    14881 gtttacacaa aaatagatgg tagttggaaa gaatcaactg gtccttattt atcgattcat
    14941 cgtgtcgctt tagcagaaag tactcgagga aaaaaactag caaaaccgtt cttagaacat
    15001 ttgttgacga ttgcagccag caaaaatatc caagatatcc gtatcgatac ttatccagct
    15061 aatgaaccga tggaaaagac catctattct gctggattca cttatcaagg gatgattgaa
    15121 tttccttttg aacacggcga aagaaaagca tatcaaataa tcatcaattg actataaaat
    15181 catctgagca aagataaaga cattaacact actgaaaaaa acaatcatat gatagccaag
    15241 aggttgtaac agaagtggcc agcttcaaaa aacaagaaag aatttccgaa aatgcttctc
    15301 aaatttttgt ggaattttag cgtattttac gaaatagctg ctttctgaaa ctcccttcat
    15361 cgagaggtcg tgacatagct tttgtcacga cctctcggct tatcctacga gtattattcg
    15421 atacatttat atcaataatc ctttttttat tttatctgca gcttcttgga acgctccatc
    15481 gtttgacacg actaaaagtg aggggatagc tttcaaacgt ttcaactgtg ctaaatcttc
    15541 tacataaacc gccagtctgg ctgaaatctg ttcagatgaa tctccacgtt ttttcaggcg
    15601 cgattgcaat gtttctttag agatcgtaat aaagatagga cggactaccg tcccgaaata
    15661 ctcatataaa tgccaaaaac catttgctgt caaaacggcg taacaatttc ctactgtcag
    15721 tttcttttta atttctgctt tactggaacc atatttatgt tgagcatatt ggtcccactc
    15781 tgcaaactca tttcccttga tcatctgatt gaatgtattg tcagaaacaa aataataatc
    15841 ctcttgatct ttttccccag gtcttggtga tcgattcgtg taagtaatga tcttttgttc
    15901 cggctgaaaa atagcttctg cgagtgaagt ttttccagaa ccagaaggac cgataaatac
    15961 atagcaataa ttcattgtca tttctccttt gatgattgaa caagtgaaaa agctttggaa
    16021 aatatttcca aagcttttaa aagtaacttt gcttttttgt atattatacc agcaagccag
    16081 tcacaatagc acttaaaaag ctgactaaag tcgctgttag caaaattttc aatccgtgtt
    16141 ttgcgacaag atttcctttt tcttcattta atcccttcat cgcaccagaa atgatcccga
    16201 tagaagagaa gttggcaaat gaaacgagga agacagatat aattgctgtt gtacgctcag
    16261 taaaatggaa attaccattt gctaattctg tcatagcaac aaattcattt gtaaccagtt
    16321 tagtcgccat gatactaccg gcatccactg cttctttcca tgggattccg ctgatgaagg
    16381 caagaggggc aaagatgtaa cctaggatct cttggaaagt gatgccgaat atcccgtgga
    16441 agatagcatt gatcatggca atcaaagcaa caaaaccgat aagcatagca gcaaccgtga
    16501 tcgccacatg gaaaccatca agaatgtatt ctcccaacat ttggaaaaat gtctgttctt
    16561 tgttttcttc aatgatcagc tcatcttctt tttcttctaa tacataggga tttacaattg
    16621 aagccagaat ataaccgcca aataagttca aaacaagggc agtgatcaca tatttagaat
    16681 cgatcagtgc catataagat ccgacgatcg acatagaaac tgtagacata gccgatacac
    16741 tcatggtgaa caaacgtttc tcagatagga agggtaattc cttttttatg gaaataaaaa
    16801 cttccgattg tcctaaaata gcagaagcta ctccattata agattccaat ttgcccatcc
    16861 cgttgatttt acttaatcct agaccaacag ctttcataaa taatggaaga atcttgatat
    16921 agcgtagaat accgataata gcagaaataa atacaattgg catcaatacg gaaagaaaga
    16981 aaggtgcact tcctttattt gctacaccgg caaaaacaaa gtcaacacct tgcccagcaa
    17041 aagcaagaag atgatcaaac atttttgcca atacagaaac aaccgctgta ccaaaagttg
    17101 tacgtaataa tacgaaacca agaacaagct gtaaaacgaa catcacaatt agtggtttca
    17161 atttgattgc ttttttgtta ctgctgatca aaaaagcaag ccctaatgtg acaaataaac
    17221 caataatacc taataaataa ctcaaataat cactcctatt ccaaattccg ttttatttta
    17281 cctgttttgc aaataaaata ctaatcttta gtgaaatttt cattactttt ttagagtgaa
    17341 acgcattcat tttccattag aaaataaagc gaacaaaata aaaggtttaa aaagtgtaac
    17401 gtcttagtaa agtctataaa aaaacgaaac tatcgattaa gctaatagtt tcgttttcaa
    17461 catttataag taaacttttt ctattggtaa tatagatttt cggaagggta gacaggtgcg
    17521 caggtaatat cgattcctag ttttttcaat gtttgttcat catttttatt gaccatcaca
    17581 gtagagtggg cttgtgctcc ctcaagttca gctagtttat cataagcaac ttgagcgatt
    17641 ggattcgtta ctgcactgat tgataaagcg atcaagactt cattgatgct tagtgcatcc
    17701 aaagaggaat gaagtgtctt tgatttcaat ccttgtattg tttcaagaac taatggtgac
    17761 aataaaggaa tgtcatctgc gatatgggct aaatgtttta ctgcatttaa gataactgcc
    17821 gctgacgcat ccatcaatga acttgtacgc ccggtaatga tgacttcatc tggtaattcg
    17881 attgcaatca cagcagaagg atcttttgta ggactttgcg cttgaatctt atctgcatag
    17941 tcatgggcag ggactaccac gtttcggtct tcaggacgta aaccgacttc ttccataatc
    18001 agtttgattc gattaactgc ttcttcgtcg atcaatcctt ttttgaagtc gcattctgtt
    18061 tggaaataac gacggatgat ttcttgttta gaggcttctt ggacaacggt atcatccacg
    18121 atcccaaatc ctacacgatt cacacccatg tcagtaggag attgataaac agattcttta
    18181 ccagtaattt tttcaataat acgtttgatt acaggaaatg tctcaacatc ccgattatag
    18241 ttcacagcta ctttattgta ggcatcaaag tggaaggaat cgatcatatt gacatctttt
    18301 agatcgactg tggctgcttc ataagcaatg ttcagcggat gtttcaacgg aacattccat
    18361 actgggaaag tttcgaactt ggagtaacca gctgcatgtc ctttttggct ttcatgatac
    18421 agttggttca aggaagtagc gagctttcca cttcctgggc caggagctgt cactacaacg
    18481 attggtttag ttgtttcaat atactcattt tttccaaaac catcttctga gacgattttt
    18541 tctacgttga ctggatagtc ttcgatttct gagtgtttat agaccttgat gtttcgtctt
    18601 tctagtttat tgataaagac tttcgtggca ggctgtccac tatagcgagt gataacgaca
    18661 ctgttgatta ataagccata accacgtaat tcatcgatta accgcaagat atccatatca
    18721 taagtaatcc cataatcacc acggatctta ttacgttcaa tatctccagc atagacgcaa
    18781 atcagtattt cagcttgatc tttcaatttt tgcagcaatt tgattttcgc atcttcttca
    18841 aatcctggaa gtacacgttt cgcatgcttg tcgtcaacta gcttcccacc aaattctaaa
    18901 tataatttgt catagtggtt cactcgttcc aaaatatacg ctgactgctc ttcaatatat
    18961 ttttctgaat caaaaccaat ttttttcact aggttccctc cttaagtact aaagcatttt
    19021 ttcatttttt tgaggattcg tcaactggaa gagcgaaatt cctcaaaaaa acaaagaaaa
    19081 tggtttaaac caacttgtct agatagttat tcattggtat gatagttttt gataaggaag
    19141 gtgctacatg aaaaaaagac agaaaaagaa aaacgcatat aaacactata tccgttcgat
    19201 ttttaccggt tatgaaagaa tgcttgaaga tcccgaattg gaacaattga cgtttactta
    19261 tctaaacgaa gaaacgcagc taactcgtga tgagcatcag cgcattcatt tcacaacaag
    19321 agatctgcct agtaaataaa aaataagata aaggtcatcg gattcgattc tgtcctatca
    19381 gatggaaaga ctagttgatc gaaaaaaagg ctgtaccaaa ttttggtgca gcctgtttta
    19441 tggagcattg cccgctcttt tttataacaa ttttttacag aaaatggtac tattgaagta
    19501 ttaataagtg aaaaacagat gagtaagagg aggcaagtag tatgaaagag ccagatacaa
    19561 atgcaaatta tgagccagac gaactagagg agacgaatgc ggatcaggat acaggacaag
    19621 aatcgtcttc tttttacgat atcaaagaaa aagcaagcat gaatctttcc caattattag
    19681 aagacttaca ggcttttgaa aaagctgtcc gcgaagaaga tatccctgag atctaccgtt
    19741 tatacaatgg ccgattgaat gaagaattaa aaagaagctc aaatgaaaat cacgagatcg
    19801 atcaattgct gatgcgtaag atccatgatc gttttttaca ggaatttcct tttatggaac
    19861 aagtagaaaa catctcgcca acgatgagct attacaaaat cggcacttat taccatgatc
    19921 ggcccacggt cggtattgat gcaagtctgc cagaaatttt tgttttgcct aagatcgatg
    19981 aagagtggga gaaatattcc caaccggagt caacggacta tgacaaaaaa atcaatgagc
    20041 tagacgcaaa ggtgatcact gctcagacag aaattgaacg tttagatgaa caaataaaag
    20101 aaatcaatcg acaaatgact gatttgaacg acaataaagg attcttgaat cgtaaaaaaa
    20161 tcgaggaaga aattcaagaa ttagagaaga agaagcaagt actatctaat gaaaaattag
    20221 gctggcttcc ttatattgag acgcctgaaa caatccagca gcaaaaagaa gcattgaagc
    20281 aagaagcaag agcagaccag cttcgtgcag ccatcgtaga aaaagaacaa aggcaaatca
    20341 gacgctattt cggtagcaaa gagggattcg gtcaagcaat ccatgaattc ttgatgaatt
    20401 atcttggcaa tgagaatcct aataatcaat ctaatgaagg aggttccgag tatgaatgaa
    20461 cacgatgaag cagaatggac aaaaaatgaa gaacaaacag aagatcatga agaaacgaac
    20521 acttcattgg aagaacaaac aagtaatcct ctggatgaca cagaagaaat ccctatgcta
    20581 gaggaaacaa aaatacacga aaaagaaaaa gtaactccga cgaatacgaa tactgcagaa
    20641 aacacccatg cttacgaaat aactgaaaat cccgggtatt tagaaggaaa agagcaagat
    20701 catcaagata tcgtcagttt gattgaaaca gcggaagcag aattagcgga tttacgtatg
    20761 agaaaagatt tgatcgaatc agattttcca caattattag aatcatatga tcgttttata
    20821 ttgaatgaag aaccaatctc cacgatcgga aagacaagcc tgatcgaata cttcacgtat
    20881 gaattactga aaccgttgaa tgatattggt ctggaacggt caaattcagc ttatgacaca
    20941 tggaaaacaa gacatttttc gatcaattat acgttgaatg gacaaaacga attagtcttt
    21001 tcctttgtat taccgaccat cgatcaacga taccaagtgg aatcgatgag tctcctaaac
    21061 gtatcaccag agacaatgga aatcaatgtc caagatgacc gggtgctttc tttgatccga
    21121 cactggtcag tggatcgagt attctctgca gggcagatca cgatattcaa tcacaaattg
    21181 aatcaattat tagcccatgc aagaacactt ggttttaccg ttaatcagac attactagac
    21241 aatacgaagc cacttcgttt gaatcttcag agcgaatttg aattgacgaa tgaagtattg
    21301 gatgatattt ttatcgtggc aatgaaaaat ccatcctacg atatggaaaa gatcaacgaa
    21361 actacttatc aagtacgatt agataaagga cagtctttaa cgataacaac aaacgaaaaa
    21421 gatcaaacag tcttgtcgat ctcatctgga gactacccac gatctgtcat tgatttcttt
    21481 atcaattacg aattcttggt cccattaatg gtgagaaagt cctgattgga taagttggat
    21541 taccagatgt cattcagata attctgttcc aaaggacttg cggcagttat ccagaataat
    21601 tgtcacaagt cttttctgta tgtactaaat ggacgtgaat aacttgctga tgaaggaggg
    21661 atttcattag gaatgtccac aaataaaata gcacagctta gcttattgtc tgctgcttgt
    21721 gtagcaggta ggatcgcttt tcaatttatt ccgaatttcc agccaatgac tgctattttt
    21781 ttatttatag tactttactt aagcttaaaa gatgcgttaa ttgtcatgag tctttctatt
    21841 gcgatcagca gtttttattt tggtgttgga ccatgggtca tcggccaatg gatcagctat
    21901 actgttgttc tgagtttgtt ttcaatcgtc tgcttgagaa aaacagtgca aaggaatcta
    21961 tggttaaagg gagtctgttt ttttctggcg ggcatggttt acggaatagg tatgaccgtt
    22021 tttgatacgc tgttatatcg tttgcctcaa ccttggatct attaccttca aggggtgtcg
    22081 tttgatttga tgcacagtat aggcaatgtt gtgttctttc ttgtttttct gccagtcgtc
    22141 aaacgttttt acaatcattc aggaggaaaa aatgaaaaaa ataatttata gtttgcttgc
    22201 aatgagtgtc agtatactca ttttgacaag ttgcagtaca acctatacac aatcggcttc
    22261 caaccaaaag gaggaagtaa aaacatcgca aaaagcaaca atcacgttac aagaaaatgg
    22321 tcaaaacttt tcggaaaaag aagtgttctt caaaaaaggc gatgatttac tgagtatttt
    22381 gaaacgaaat tttgaagtca aagaagataa cggcttcatc acttcgcttg aagggcactc
    22441 acaggatgag aaaaatcaac tctattggat gtttactgtg gacgggaaaa gtgctactac
    22501 tggtgccaaa gagatcaaat tgaatgatgg acaatcggtc attttcaatt tgtctaaact
    22561 ataagtttca taaaaaactg ttaaagggcg aatattgcca taattcagtg agttcagttg
    22621 atgaaagaag gaaaataaca tggaaaattt taaaaaaagc ggaatgtttt ccgtaattgc
    22681 tgctctaggg gctaatattc tagttgctat cagcaaattt gtcggctatg caatcagtgg
    22741 cagtgccgcc atgctaaacg aaagcatcca cagtatcgtt gattgcagca atcagatttt
    22801 gctgttattc ggtgataaac aagccaataa aggacagagc gagttgcatc aatttggtga
    22861 aggacgagca aaatactttt tcagtacaat cgtcgcaatg atgcttttct tcggcggtgg
    22921 ggcacttggg gtgatggaag cttttgaaaa gttgacgcat ccatcccatg aagtgggtaa
    22981 tccgatcatt gttatagcaa tactgttatt tggtttggta atcgaaggaa gttcgttacg
    23041 cgtggcaatg aaagaaatca aagaattgaa tactgaaaaa ctttccacga tgcgcttttt
    23101 gcgcgaaagt cgacacagtg aaatactgat tatttttacg gaagacttgt gtgctgtaat
    23161 cggtttggtc ttggctttag caggaacaat attgacttat gcaaccggga ttgctgcatt
    23221 tgatgcgatc agcggattgc tgatcggttt cttgctgatg ggagccgcca ttttcctagc
    23281 aaaagaattt tatagtctga tcatcggtga aagcgtgaca gcaagcgatt tatcaaaaat
    23341 caagttagct tttgaacgtc ctgagatcag tcggctgatc gatataaaaa cagttcattt
    23401 aagtccaacg gagattttag ttgcagcaaa agcagatatc gttggtaaca aagagaatga
    23461 agcttactcg gtcatcaatg atattgaaaa aaatatgcgt cagtctcttc ctgacaagaa
    23521 gatgtatata tacattgaaa cagataaata tgacccaaat tattctcgta aataaaacgt
    23581 ttattcatga attgaagcaa aaatgagaaa aatataat
//
LOCUS       DAYYHX010000022        23220 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000022 DAYYHX010000000
VERSION     DAYYHX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.022"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            363..731
                     /locus_tag="ACHA6Y_000647"
     CDS             363..731
                     /locus_tag="ACHA6Y_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM8986763.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="ACHA6Y_000648"
     CDS             911..1852
                     /locus_tag="ACHA6Y_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM8986764.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="ACHA6Y_000649"
     CDS             1921..2652
                     /locus_tag="ACHA6Y_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8986765.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="ACHA6Y_000650"
     CDS             2796..4976
                     /locus_tag="ACHA6Y_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM8986766.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="ACHA6Y_000651"
     CDS             4996..5478
                     /locus_tag="ACHA6Y_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM8986767.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="ACHA6Y_000652"
     CDS             5494..6255
                     /locus_tag="ACHA6Y_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8986768.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /gene="murC"
                     /locus_tag="ACHA6Y_000653"
     CDS             6386..7720
                     /gene="murC"
                     /locus_tag="ACHA6Y_000653"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM8986769.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="ACHA6Y_000654"
     CDS             8027..8512
                     /locus_tag="ACHA6Y_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM8986770.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="ACHA6Y_000655"
     CDS             8505..9188
                     /locus_tag="ACHA6Y_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM8986771.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="ACHA6Y_000656"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="ACHA6Y_000656"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM8986772.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="ACHA6Y_000657"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="ACHA6Y_000657"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM8986773.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /gene="coaE"
                     /locus_tag="ACHA6Y_000658"
     CDS             12804..13433
                     /gene="coaE"
                     /locus_tag="ACHA6Y_000658"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM8986774.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="ACHA6Y_000659"
     CDS             13485..13817
                     /locus_tag="ACHA6Y_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8986775.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="ACHA6Y_000660"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="ACHA6Y_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM8986776.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="ACHA6Y_000661"
     CDS             14643..16016
                     /locus_tag="ACHA6Y_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8986777.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="ACHA6Y_000662"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="ACHA6Y_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM8986778.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="ACHA6Y_000663"
     CDS             17071..17511
                     /locus_tag="ACHA6Y_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM8986779.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="ACHA6Y_000664"
     CDS             17655..18356
                     /locus_tag="ACHA6Y_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM8986780.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="ACHA6Y_000665"
     CDS             18353..19474
                     /locus_tag="ACHA6Y_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM8986781.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="ACHA6Y_000666"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="ACHA6Y_000666"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM8986782.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="ACHA6Y_000667"
     CDS             20853..21761
                     /locus_tag="ACHA6Y_000667"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8986783.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="ACHA6Y_000668"
     CDS             21796..22068
                     /locus_tag="ACHA6Y_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM8986784.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..23125
                     /locus_tag="ACHA6Y_000669"
     CDS             22079..23125
                     /locus_tag="ACHA6Y_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM8986785.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7820 a         4044 c         5085 g         6271 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    23161 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
//
LOCUS       DAYYHX010000023        23116 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000023 DAYYHX010000000
VERSION     DAYYHX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.023"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            242..2713
                     /locus_tag="ACHA6Y_000670"
     CDS             242..2713
                     /locus_tag="ACHA6Y_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8986786.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2816..4552)
                     /locus_tag="ACHA6Y_000671"
     CDS             complement(2816..4552)
                     /locus_tag="ACHA6Y_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8986787.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4549..5253)
                     /locus_tag="ACHA6Y_000672"
     CDS             complement(4549..5253)
                     /locus_tag="ACHA6Y_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8986788.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5384..5887
                     /locus_tag="ACHA6Y_000673"
     CDS             5384..5887
                     /locus_tag="ACHA6Y_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM8986789.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5847..6185
                     /locus_tag="ACHA6Y_000674"
     CDS             5847..6185
                     /locus_tag="ACHA6Y_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM8986790.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6206..7624
                     /gene="ffh"
                     /locus_tag="ACHA6Y_000675"
     CDS             6206..7624
                     /gene="ffh"
                     /locus_tag="ACHA6Y_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM8986791.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7736..8314
                     /locus_tag="ACHA6Y_000676"
     CDS             7736..8314
                     /locus_tag="ACHA6Y_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM8986792.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8318..8839
                     /locus_tag="ACHA6Y_000677"
     CDS             8318..8839
                     /locus_tag="ACHA6Y_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM8986793.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8918..9472)
                     /locus_tag="ACHA6Y_000678"
     CDS             complement(8918..9472)
                     /locus_tag="ACHA6Y_000678"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8986794.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9725..10000
                     /gene="rpsP"
                     /locus_tag="ACHA6Y_000679"
     CDS             9725..10000
                     /gene="rpsP"
                     /locus_tag="ACHA6Y_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM8986795.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10012..10263
                     /locus_tag="ACHA6Y_000680"
     CDS             10012..10263
                     /locus_tag="ACHA6Y_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM8986796.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10388..11179
                     /locus_tag="ACHA6Y_000681"
     CDS             10388..11179
                     /locus_tag="ACHA6Y_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986797.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11349..11870
                     /gene="rimM"
                     /locus_tag="ACHA6Y_000682"
     CDS             11349..11870
                     /gene="rimM"
                     /locus_tag="ACHA6Y_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM8986798.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11860..12621
                     /gene="trmD"
                     /locus_tag="ACHA6Y_000683"
     CDS             11860..12621
                     /gene="trmD"
                     /locus_tag="ACHA6Y_000683"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM8986799.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12757..13104
                     /gene="rplS"
                     /locus_tag="ACHA6Y_000684"
     CDS             12757..13104
                     /gene="rplS"
                     /locus_tag="ACHA6Y_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM8986800.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13477..14139
                     /locus_tag="ACHA6Y_000685"
     CDS             13477..14139
                     /locus_tag="ACHA6Y_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986801.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14218..17445
                     /gene="fss3"
                     /locus_tag="ACHA6Y_000686"
     CDS             14218..17445
                     /gene="fss3"
                     /locus_tag="ACHA6Y_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8986802.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            17559..17873
                     /locus_tag="ACHA6Y_000687"
     CDS             17559..17873
                     /locus_tag="ACHA6Y_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8986803.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            17886..18260
                     /locus_tag="ACHA6Y_000688"
     CDS             17886..18260
                     /locus_tag="ACHA6Y_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8986804.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18270..18614
                     /locus_tag="ACHA6Y_000689"
     CDS             18270..18614
                     /locus_tag="ACHA6Y_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8986805.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            18685..20037
                     /locus_tag="ACHA6Y_000690"
     CDS             18685..20037
                     /locus_tag="ACHA6Y_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8986806.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20097..21239
                     /locus_tag="ACHA6Y_000691"
     CDS             20097..21239
                     /locus_tag="ACHA6Y_000691"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFM8986807.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21264..21518
                     /locus_tag="ACHA6Y_000692"
     CDS             21264..21518
                     /locus_tag="ACHA6Y_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986808.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            21774..22958
                     /gene="mobT"
                     /locus_tag="ACHA6Y_000693"
     CDS             21774..22958
                     /gene="mobT"
                     /locus_tag="ACHA6Y_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8986809.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            22955..23092
                     /locus_tag="ACHA6Y_000694"
     CDS             22955..23092
                     /locus_tag="ACHA6Y_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8986810.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7685 a         4108 c         4972 g         6351 t
ORIGIN      
        1 gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga aaaagtgtta
       61 gtagaaaatc gccttcgatt ttccaccaac acaaaatatt atagaaccct ttttttttgc
      121 tatatgacag gaatgaaagg tgtttgcaag cttaatgtga ggaacacttt ccatatctgt
      181 tctatactgg ataggagaag tttggaaata tccagaaaac gaaatcaatc gaggtagcca
      241 catgaagaat aaatggataa atcgaagtaa aaaaaagaaa gtaacacaaa gatcatcaga
      301 taaagtcatc cgtctttttc catcgtataa agaaggatta acggcagatc aagtcaagga
      361 acgaatagaa aaaggtgcag caaataattc agtcgatccc acattcaaaa caaatcagca
      421 aatcgtattg gaaaacatat ttacgtattt caatttgatc tttcttatct tagccatttt
      481 gctttgttta gtagaatcct ataagaactt gactttttta ccagttatca tagccaatac
      541 aggaatcgcc atttttcagg aaattcgatc caaaaagatc ttggacgaac taaatgtttt
      601 aaatacagca aaagtcaatg tcatacgaga cgggatagaa caaacaatag atatagaaga
      661 ccttgtaata gatgacattg ttattttaga gactggatat caaattccag cagatgcaga
      721 ggtcgtggaa gggaaactgc aagttaatga ggctttattg acaggcgaag cagatgaaat
      781 aaccaaagaa ataggtgatc cattaatgtc cggcagtttc attgtttccg gaaaagctta
      841 cgcccgatta gacaaagttg gagcagattc gtatatttcc cagctgacag tcaaagccaa
      901 gacaatggga gaaggagaac agtcggagat gattgcttct cttaatcaat tgatcaaatg
      961 gattgggatc atcattattc ctatcggtat cctgcttttt tctcaaagtt acttttttaa
     1021 tggaaacacg atcaaagaaa gtgtcgttgc aatggaagca gcattgatcg gtatgatacc
     1081 tgaaggactg tacttgctga cgacgattgc attagcatta agtgcgacta aactagcaaa
     1141 gcaaagagtt cttttacaca atatgaaaag cattgagaca ttggcaaggg taaatgtcct
     1201 ttgtgtagat aaaacaggaa cgatcacaga aaataaaatg tctgttcaaa aaatgatcgt
     1261 ctctaaaaaa caagaaataa tcagtcagtc agatcaattg gaagcatgca ttgctgacta
     1321 tgcaaaagcg atggcaggag acaatgcaac aatggaagct gttaaagcat attttacaaa
     1381 aaccactgat caatcctatc accatgttat tcctttttct tctgtccaaa aattcagcag
     1441 tgtagcgttg gcagacaagg tgtatgtttt aggagcgccc gaaatggttt tgcgcgatca
     1501 attgccggaa tatgcggaag agtttgtttc tttcgctgaa aaaggctatc gcgtgttggt
     1561 ctttggtgtg tacgatggga ttcttgaaga accagtattg acagaagcag tcacaccatt
     1621 aggctatatt ctgattgcta atcctatcag aaaagaagca agagccacat ttgattactt
     1681 caaaaaacag caagtagcca tcaaagtgat ttctggagat aatccgctca ccgtatccaa
     1741 tgtagctgtc caagcaggga tttcacatgc tgatcagtac gttgatgcat ctaaattatc
     1801 agaagaagaa tatgaagcag ctgtcgaaaa atatacagtt tttggacgcg tcaagccaga
     1861 acaaaagaaa atcttcgttc aattgataaa gcgtaacaat acagtagcta tgacaggtga
     1921 tggcgtgaat gatattctag ccatgaaaga agcagactgc agtatagcga tggcttccgg
     1981 aaatgaagcc gccatgcagg cctcacaagt ggtcttactt gattctgact tttcacgcat
     2041 gccagaagtt gttgcagaag gacggcgcgt tgtgaacaat attgagcgat cagccagctt
     2101 gtttttagtg aagaatattt tctccttttt gctctcactg ttttcagtga tctttgctct
     2161 gacctatcct ttagaaccat cacaaatcac attgattagt ttgtttacta tcggacttcc
     2221 atctttcttg cttgctctag aagaaaacaa aaagcgaatc agagggaaat tcatcatgaa
     2281 tgtgatggaa aaagctgtgc caggtggact aacagatatg atcgtagtcg gtgcgttggt
     2341 gatctgcggg gtgactttga atctaaataa aacagatgtt tctacagcat ctaccatgct
     2401 tttgattgca gtagggttcc tcgttctcta taaaatctgt tcaccgctga ataaattcag
     2461 aagtcagatc attcttttct gtgctagcgg taactttttt tcggtcatct ttttgcataa
     2521 gctgttttca atcaccggta tctcagctgt atcacttttg ctgttgagca ttctattttt
     2581 ctcggcagat tcgattttta ggcatttgac cacattggta gaatatttgt ttgaacgtaa
     2641 aaatgaaaaa gagcaaaagc cgtttagtcg cgtagctaaa aaatttctat cgatatttaa
     2701 acgagaaaat tgacgaaaag tatctcaaaa aagagtaaaa aagaacaacc actcgtgtat
     2761 ctttcgaagc aggatgagtc atccaacttt aaaacgcacg agcagttgct cttttttaca
     2821 ttttcggaat ggtaacggtg aaagtcgaac ctgttccaag atgactgtct acagtaatcg
     2881 taccattaag gagttgcacg tagtctttta caatcgctaa ccctaatccg gtcccgcctg
     2941 aatgtcggct tcgcgccttg tctacgcggt aaaaacgctc aaagatccgt tcctgatcct
     3001 caatgtcgat acctatacca aagtcttgga cagaaaacga tagatcatct gttgcctgat
     3061 agcggataat aatctcgctg tcgctttttg aatattggac agcattttca attagatttt
     3121 tgataattgg ataaaggata tctgttttag tagtaaatgg ttcgttcttt ccaatcaatc
     3181 gaatcgtcaa ccgtttcttt tcgatgattg gctgataatt ttgaacgatt tgctggaaat
     3241 aaggttctgt ataaattgtc tggatctcgt aagaattctc gccatctttt gacagctgga
     3301 tgatttcccg aatcagttta tctaaccgga tcgcatcttt ttgcatgatc tcaagaaaag
     3361 aagtcagagt ttgcgggtca tcttttgcac catccagtaa agtctcggta aaaccaatca
     3421 gtgaagtcac aggagttttt aattcgtgag agacgttgcc aacgaaatcc ttttgtagct
     3481 tttctaaacg tcgcactttc gttaagtcat acgccacccc tagaatctgt ccggtacctt
     3541 ctgaattagt aaagtagcgc aaactcaagt ctaatgtctg ccgaccttct gtaagggtga
     3601 tctcctgatg gatcagcgga tgctcaggag tcacttgatg aatcagctga atgagttttg
     3661 gctcttgaat gacttctgtg taccgctgtt caggttgata gcttaccacg tttaaatgta
     3721 gctgcatctt tggattaagc agtagtaaac gagaatcagc atcatcaatc aggaatacgc
     3781 caatcatcag ttcgttcagt aacgtatata attgctcttc tgtggtagta cacgcccgat
     3841 acatcttact catttgttcg ctcaacgcat taatcgtctg atacaattct tcccattgat
     3901 cgggtgtttg catgattact tccgcctgat taggattttg caccattttt tttagtacgg
     3961 gcagcacggt tttcaatggc tggttttttt gatagatcag ataatagatc attcctgcaa
     4021 gaacaaggaa gaaagacaga aagaagaaaa agacccaacg gcgaaaactc tcagttctcg
     4081 gtaaaaaacc gcttgtcggt tctgcgatac gtaaaatggc ttctaactgg ccactttttt
     4141 tgactggtaa tgcgacatat aaaagatcct cattcaaagt agtacttttg cgcaaagcgc
     4201 tacctaggtt cccaccattc aatacggctt tgatttcggg acgggagttt cgctgttcat
     4261 tcaatgtttc attgtttgtg tcaaataaga tctttccagt agcatccatt aaagtaatcc
     4321 gttcgttcga ctgatggacg aattcttcca aagcagtttt gttttgcgct gcttggaggt
     4381 tttcgacatc taattgccgg atcagcagtt gtgctttttt tgtcaggtag ctttcttgct
     4441 gttccacgat ctgctgttga aaataatggg aaatcaattg ccagccacca aaaaacaaac
     4501 taactaaaag aataagaaag aagagacgtt cataaatttt tttccatttc atcgtttggg
     4561 ttcctgaaac ttatagccaa atcctctaat agtcaccaag tactttgggt gttttggatc
     4621 tcgctcgatt ttttctcgca aatgactaac gtgtacatct acgattcggc tttgtcctgc
     4681 gaagtcaaaa ttccaaatgc ggtctagcaa tgtgtctcgg tcgatcacac ggtcttttcg
     4741 tttcatgaaa taaacaagta attcaaattc tttcggtgtt agttcaatca gatggtcgtc
     4801 tacggtcact tgataattgg tcaaatcagc agtgatttgt ccaatcgata gatattcagg
     4861 ctcagcttca tcttttgatt gcttacgagg ttcaattcgt ctgaatattg ctttcatacg
     4921 ggctaatact tcgcgaggac taaatggttt ggtcaaatag tcgtcagcac ctatttctag
     4981 gccaatgatc cgatccactt ggtcatcttt agccgtcagc atcaaaatcg gtgtttcatt
     5041 tttttcttga cgcagccgct gagtgatctc catgccatcc atcgttggaa gcatcacatc
     5101 taaaatgatg aaatcaaatg attgttctaa tgctaattct aatccttcgc ctccatctgt
     5161 ggcactggtt accttgtagc cttctttttc caaattgaac gtcaagagcg ttacaataga
     5221 aggttcatca tcaacaacta gtattttttt cattcaaatc tcctttaaat aggtttcatt
     5281 ctgtttatat tttagcatag aatcagtaca aagggctatt ttacaaaaga aataaatggt
     5341 ataataggaa ggcactttgc tgaatagaaa ggaagagcag tcaatgattg gaatcagtgc
     5401 atgtttaggc ggtctggcct gtcgctatga tggacagaaa aaagaagtac cagaattgat
     5461 ggaactagtt gaaactggag aagctgtcat ggtctgtcca gaagtgatcg gcggattacc
     5521 aattcctaga gatcccgctg aaatcattgg tggaaatgga ttcgatgtgt ggaaagatca
     5581 agcaaaagta tggacgacaa gcggcgaaga tgtaacggaa gcgtacaaaa atggcgcgat
     5641 caaagcctat caaaaattgc aggaaaaaca cattgatcta gtcatcatga aggaaaaaag
     5701 cccttcatgc ggaacccatt cgatttatga cggaagtttt tccggaataa aaaaaaatgg
     5761 tgccggagtg gctactgctt attttattca gcaaaaaatg actgtgctct ctgatgaaga
     5821 atggctgaaa actagaggtg agcagaatgg aaatcgaaaa gaccaatcga atgaacgcgc
     5881 tctttgaatt ttattcgacc ttattgacag aaaaacaaat gaactacatg gaattatatt
     5941 atgcagatga tttttcatta ggtgagatcg cggaagaata cgaagtaagc cgacaagcag
     6001 tatatgacaa tatcaagcgg acaagcaaga tcttggagga ttatgaaaaa aaactccatc
     6061 ttttttctaa ttatattgtt cgagaacagg tattggaaaa tatgactgca tatattacag
     6121 ataagtaccc ggaagataaa aaactacttg aatatgtaca gcaaattcaa gcaatcgaag
     6181 aataaataaa aggaatgaag aaaatatggc atttgaaagc ttaactgaac gtctccagca
     6241 ggcgatggga aaaataagaa aaaaaggaaa agtatcagaa gctgacgtaa aagaaatgat
     6301 gcgggaaatc cgccttgcct tactagaagc cgatgttaac ttgcaagtag tcaaagattt
     6361 tactaagcgc gtacgcgaac gtgcggtagg ggcagaagtg ctggaaagtt tatcacctgc
     6421 acaacaaatc gtaaaaatcg ttgatgaaga attgacgatc acacttggtt ctgaaacagc
     6481 agagttaaat aaatcaccaa aaataccaac tgttatcatg atggctggtc ttcaaggggc
     6541 aggtaaaacg acctttactg gtaaattagc caattattta aagaaaaatg aaaatgctcg
     6601 tccgctccta atcgcagggg acgtatatcg accagctgcg atcgaccaat tgaaagtatt
     6661 aggtcaacag ctagacgttc ctgtatttga tatgggaact gaagtcagcc cggtagagat
     6721 cgtccgtcaa gggatggagt tagcaaaaga aaagaaaaac gactatgtat tgatcgatac
     6781 ggcaggacgt ctgcatatcg atgaaacatt gatggatgag ttgaaacaaa tcaaagaatt
     6841 gacgcaacca aatgagattt tgcttgttgt cgatgccatg actggtcaag atgccgtcaa
     6901 cgtagctgat agcttcaacc agcaattagg tattacaggg gtcgtcatca ctaaacttga
     6961 tggggatact cgaggcgggg cagcactatc gatccgctca gtgacaggcg caccaatcaa
     7021 attcatcggt tctggtgaaa aattgactga cttagaagta ttccacccag atcgtatggc
     7081 tagccgtatc cttggtatgg gggatatgct gacattgatt gaaaaagcgc agcaagacta
     7141 cgacgagaaa aaagcggaag aacttgcaca gaaaatgcgg gaaaattcat tcgatttcaa
     7201 tgactttatt gaacagcttg atcaagtgat gggtatgggc ccactggaag atctaatcaa
     7261 aatgatccca ggaatgaatc aagtccctgg aatcgaaaac gtcaaggtag atccaaaaga
     7321 tgttgaacgc aaaaaagcga tggtttattc tatgacgcca gcagaaagag caaatcctga
     7381 tctgttgaac ccaagccgtc gtcgccgaat tgctgccggt tctggaaaca gtgttgttga
     7441 agtgaatcga atgatcaaac aattcaagga atcaagaaaa atgatgcagc aaatgtccaa
     7501 aggggatatg aacatcccag gtatggacca aatgtttggt tcaggcgtca aaggaaaact
     7561 aggcaaaatg gcgatgaatc gaatgatcaa gaaaaataag aagaaaaaga aaaaacggaa
     7621 ataagatgaa aaaaacagct tcggtacaga ctgaaaatgt ctactgccgg agcttttcta
     7681 tcagcaattt ttattcttta ctaatacaat tactctaaac gaagaggtgg cacagatgag
     7741 atgtccgtgg agtgaacaaa cagaaagtat gcggcaatat catgatactg tctggggtgt
     7801 tcctgaatat gatgaccaaa agctgttccg aaaattgatg cttgatatta atcaagccgg
     7861 attgagctgg cagacgattt taaataaaag tgaagcattc gatgaagcat atgatcgatt
     7921 cgatatcaaa aaagtcgcaa attatggaga aacaaaaatc agtgaactaa tggaaaacaa
     7981 aggaattatt cgtaaccgaa gaaaaatcga agcagccgtc caaaacgcca aagcagtctt
     8041 gaaaattcaa gaagaaatcg gcagtttttc agagtatctt tggtcgtttt ctaatggaaa
     8101 aatcgttgat tcttcttatc attcacaaga agaagtccca acaacaagtc ctctttcaga
     8161 tgaattagca aaagatctaa agaaaagagg atttaaattt atcggaagca caacaatcta
     8221 tgctttttta gaagctgtcg ggatcatcaa tgatcacctt gacagttgtt tccgcaaaga
     8281 agaggtcgta cgtatcggca gaaagggcga ataaagcatg cgaaaaatca tattttatgg
     8341 tgctataagt ttagatggct atttggcaac gaaaacggat gatcttcaat ggctttttga
     8401 cactccaaca ggtaagcaaa ctacttatga agctttttat gaaacaatcg atacgaccat
     8461 catgggcaga aaaacttatc aggaagcaaa aaaatatttg gagaccgaag caatttatcc
     8521 agagaaaaag aatttcgttt tttccaccca cgcacaatta caattgccag atgctgaggt
     8581 cgtaaacgaa gatcctgttg cttttctaaa aaatctacaa atgacagaag gaaaagatat
     8641 ctgggttgtg ggtggcggtc gattgatcaa accgctattg gaacatcaaa tgattgatga
     8701 atggtatatc cagattaccc ctgtattatt aggggacggg attcctttat tccaaaaagg
     8761 aacatatgaa gcgcgttttg aaatgctaga cacgacacga ttcggtgaat ttatcgaatt
     8821 gcattatgta cgaaagtaga agtaaataaa ataaaaaagg caactatatg cagaaatgtc
     8881 ctgtacatag ttgccttttt tattaccgtt atgcttttta ctctgttaat ttgttagaaa
     8941 aatctagata cgcatctaaa atcgtttgga agaaacttaa tgttccttca acgatattac
     9001 cattttcatc aattagattc gcaacgtttc cgatatatgc ttccggttgc tgcaaggttg
     9061 gcacattcaa aaagacaagt gattggcgta gatggtggtt tgcgccaaag ccgccgatcc
     9121 cacctggtga tgcagtaacg actaaacctg gtttatgatc ccaaacactg tgtccataag
     9181 gtcttgagcc tacatctaaa gcatttttca atactgctgg cacagaacgg ttgtattcag
     9241 gagtaacgaa aacgacaccg tctagtcccc tcacttcctc acgaaaacga gtccattcag
     9301 caggagcctg ttctggagtt tcgagatcct cattgtaaaa tggcagatca tcgattttta
     9361 caaatacact ttcgtaccct tcaggaagca atttcgcaaa agcttctgct acttttttgt
     9421 tgtaagaatc ttttcttaaa ctaccaataa aaaatccaat tttttttgtc atacgatttt
     9481 tcctccttca aactatctat aataatctta caaaagataa gtaactggag caaataaaaa
     9541 cacttactga caaaagctac atagaagata gattacttat ttttaacaaa aaagaagtct
     9601 aaaatcttct attttctaaa aaaaatcgaa tttaatcaag ctgtaaagaa aaaaagcttt
     9661 acagggtaaa aaaaaactgt tacactacat cttgtgaata aaacaatgga ggtggaaata
     9721 aataatggca gttaaaatcc gtttaaaacg tatgggttct aaaaagagtc cgttttaccg
     9781 tatcgtcgta gctgattcac gttctcctcg tgatggacgt ttcatcgaaa ctgtaggtac
     9841 ttacaatcct ttgaaagacc ctgcagaagt agttttaaaa gaagacttag ttcttgactg
     9901 gttatcaaaa ggtgcacaac cttctgacac tgtacgtaac atcctttcaa aagaaggcgt
     9961 tatgcaaaaa caccacgaag ctaaattctc aaagaaataa ggtgaccgga tatgaaagat
    10021 ttaagcgact tagtcttaac aatcgttcgt ccgctagtca cttatcctga tcaagtcaag
    10081 ttagacgtcg tagagtcgaa agacttttac gagtacaatc taacagtggc tcctgaagat
    10141 attggccgta tcattggaaa acaagggcgt gttgctaaag caatccgcac aatcgtttac
    10201 ggtgttcgga tcaatgcacc gaaaaaagtg cgtttgaata tcatcgatga taaggaaaag
    10261 taaataaacg tgctgtactc gaattttcga gtgcagcttt tttgtttttt ttattaattg
    10321 aagaaatgat tatttggcaa aacaagcaag aaagcgtatc cttataggag gagggagtga
    10381 acagccaatg agaaagaaaa tgttatttat tccatttgct ctattgttaa gcgcttgtag
    10441 ttcggttact caagaatcag acaatacaca tctatcaagc agtatatctg aaactgttac
    10501 cagtccagaa aaaagtagta ccaaaacggc ttccaccact gaacaaacaa tggctacctc
    10561 aaataatgag aaaaagactg ctttagacca gttgaaagaa caacagccaa atgttcccat
    10621 gcctctagat gtaccagtct cttcaggcta tctcaatatc gcagcgacac atacaaagca
    10681 aggttattct attttatatt acagaacaga tcgtcccctc gggttgaatg ctgacgaatt
    10741 gaatcaagaa acgcctattg caacctatct atatcaatat gggtttgctt ctagtcaaga
    10801 aacaatccaa gttttgcagc catttgagat cgacacaaat ggtcagcaag tggacttagg
    10861 ttctcgaatc acagggtatc agcaaggtgc agcaggttca agttttcttg aatggcaaga
    10921 aggaaactgg cgtattcgaa tccgtgggaa taatatagaa ggccaagatc ccttactatt
    10981 agctaaagaa atcgtagctt atttggaaga aaacagtcta ccagcacctg aacaatttgg
    11041 taaaattact gttgatatgg gtgatacgac gaatcgagcc gtagaagtaa gctggcaaga
    11101 accgaaaaat gcctatacga tcactcatca agatcctatg tctgcgttga agatggcagt
    11161 ttctatgaag aggttgtgaa atcagcgcgc tgatctgagc ccatcattta actccaagtc
    11221 acaggaagta atcaaaaata gcacgccgca tttttgcata attttggctt aataccttaa
    11281 aagaaaacta gaattgcctc ttcacacatg ctaaaataaa acagacaaaa aagaaatggg
    11341 gatataagtt gactgaatac ttgaatgtag gaaaaatcgt aaacacacaa ggtatcaaag
    11401 gcgaagtccg tgttatctca acaacggatt tcccagaaga acgttacaaa aaaggaacgg
    11461 tgctaacact attccaagac ggaaaagcac ctgtggaatt aacagtcaaa agtcatcgca
    11521 aacataaaaa ttttgactta ttgagttttg aaggacatcc ttctatcaat gatgtggaaa
    11581 aatatcggga tggcatatta aaagtgtcaa aagacaactc tgtagatcta gctgaaaatg
    11641 aattctatta ccatgagatc atcggtctgc atgtattaga agatggaact gagttaggta
    11701 aagtaaaaga aatcttgtca ccaggtgcca atgatgtttg ggtcgttcaa cgtccgaaaa
    11761 aagctgatat attgcttcca tatatcgctt ctgtcgtcaa agaagtcgat ttagaagctg
    11821 gagtggtccg tgtagaaatt ccagaaggac tgattgacga tgaagattga tgtattgact
    11881 ttatttccaa gaatgtttga aggtcctatg ggggaatcca tcatcggtaa ggctgtagat
    11941 aaaggattat tagatatcaa tatctctaac ttccgcgatt attcagataa caaacatcag
    12001 acagtagatg attatccata cggcggtgga gcaggtatgc tgctcaaagt ccagcccatc
    12061 tatgataaca tccaagccat tgaaaaagct gcaccggatg tcaaaaaaag agtaattctt
    12121 cttgacccag ctggtaaacg attcgatcag aaaatggcag aagaattttc acaagaagaa
    12181 cacttagtat tcatttgtgg tcattatgag ggatatgatg aacgtatccg cacattagtg
    12241 acagatgaag tttccttagg agattatgtc cttactgggg gagaattagg tgcgatggtc
    12301 atgatcgatg caacagttcg gcttttgcca gatgtgttag ggaatcagac ttcagctcaa
    12361 acggattctt attcaaccgg tttattggaa catccccaat atactcgccc ggcagaatat
    12421 aaagggatga aagtaccaga agtattgaca aatggaaatc ataaattgat tgaagaatgg
    12481 caattaaaag aatcactaag aaggacctat caacgtcggc ctgatctttt ggaaacactt
    12541 gaatggacac ctcaaatgac gaaattttta gaagaaatca agaaagaaga acaagaaaaa
    12601 acagcagatg aagccaagtg actttttcta aaatcttctt tacagtaggt tgataatatg
    12661 ttagtatttt atggtgagtg caaaagcact taactattac gatattccgc tgtgagaaaa
    12721 gctcataaga atatttggaa taaggagaag aaaaccatga atccattaat cgaagaatta
    12781 acaaaagaac aattacgttc tgacatccca gcgttccgcc ctggtgacac tgtgcgtgtt
    12841 cacgcaaaag ttgttgaagg tactcgtgaa cgtatccagt tatttgaagg tgttgttatc
    12901 aaacgccgcg gtgctggaat cagcgaaact tatacagtac gtaaagtttc taacggagtg
    12961 ggcgttgaac gtacattccc attgcataca ccacgtgttg caaaaatcga agtagttcgc
    13021 tacggtaaag tacgtcgtgc aaaattgtac tacctacgtg cattacacgg aaaagctgct
    13081 cgtatcaaag aaatcagaag ataaacacgg aaaacttcct tggaaatctg aaatttgtga
    13141 aaagtccttg tatatcaagg gctttttctt ttttctctat cacagtttta agccattttt
    13201 ggctgttttg gaccattttt caatcatatt cccttaaaaa ttgtcctaga ttgtccgaca
    13261 ttgactatca gtgtcgtcaa aatagggcaa aatttagtag tcagcttgac gacaaatata
    13321 atacgaaaaa taccaaaaca tttaccgaat ttagcaactt ttgaacccaa gtttctcaaa
    13381 gggtcagcta ttagcaaaaa agacaaaaca tgtacttttc tactaaaaag ttgatgtgat
    13441 aggcttctat ctaaataact attaggagtt tttatcgtga agcaaaatga tggacgtatc
    13501 atttggaaaa atcaaagtga gttaaaactg attttaacaa ttaatgaatt catcgaaaaa
    13561 cacggaatta catctagccg tcaataccag aaaaagctat cagaaaatcc aaattctgca
    13621 ccaagtatgt ggttcattaa caaaaaatat ggatcatggg agaatttatt gattagtata
    13681 ggacgagaaa atactgggta tggaaaatgg gctagaatgt cagaacaaga attattggaa
    13741 atagtagaag cctttattaa gtgcgagaag ataacctctc aacgaatgta cgaacaaaaa
    13801 tcagtaggga agaatattcc ttcacttagt accattaaaa agatgttagg agatatacgt
    13861 ccacttttta aagaaaaaaa tgatggatct cgatttactg atttcgaact gctgttagaa
    13921 ctaaaaaatg aaataatccg attaaaacta caggatgatt tatcaatgac aaagtttcga
    13981 aaactagttc aatccccaaa actaccatca gttgatacaa ttatgaaaag aacgaacaaa
    14041 aattgggagg aattgatggc agagattgga tttgattatc gaagaatcaa aatttataag
    14101 cagagaaaca acctatccaa gacaaagaaa actaaatagt atcactctaa atcagacgtt
    14161 caaaattaaa atttggacgt cttttttatt acccaaaaac gaaaggagat ttattttatg
    14221 aaaaagccaa gatttaagaa ttggcgtttg ctcgcaacac tgagtctatt atgccaaaca
    14281 attggtggat cgcttggccc aacgattgcc tttgctgatg aaattactca tccacaaacg
    14341 gtaactgtgg aattggattt ggctcaccag tatgctgtcg aaggtacatt tagcgatggt
    14401 cgtcccatgt cagaagttac cgttccgcac tatgcagttt acaatggtgt gaagcaagat
    14461 attttctgta ttgagccagg ggtgccgatt tacaatgagt tcactcctgg ttatgagaaa
    14521 aatccattgc ctgatatgtc ggagaaagcg aaattagttt ccgttttatg gaaggaagct
    14581 ggtacggatg tggatacaca tattgtggcc cagaagatga tttggcaaga agttaatggc
    14641 tacacgcttc actcaattaa acgttctgat ggtagtgcag taaatatcgc agccattgag
    14701 tcaaaaatca atcaagccat tgcggattat caaaagaaac caagcttcca taactccaca
    14761 gtgaaaaccg tgttaggtca atcgactact gtgacagata cgaacggctt gaatttatcg
    14821 gagtttgatg aagttgtcga gaatatcgca aatattgatt atcgtgtgaa cggcaatcaa
    14881 ctagtgatta ctccaaatgc caattcaaaa gagagtggtg aattaaccct aaagaagtca
    14941 gctaatacag gaacaccggt agcttataaa atggctggtc aacaaacttt gatggcgggg
    15001 gcaattgata agcccaatac ctacacaatc aaaattgatg tggaaactga gggttctttg
    15061 aagattaaaa aagtcgataa agaaacagga aatgttgtac cgggaaccgt tttccattta
    15121 gactttggaa aaacattacc tgcaaaagac gtgaccaccg ataaagaagg cattgcgaca
    15181 ttggatgaga ttcctcatgg cgcgaaagta accattactg aaaaatcagt gccagctcct
    15241 tatacgattg acactacacc aatcaccgca accattaaat ctggtgagac tatttctgtg
    15301 acttcaaaaa atactcgcga aaaaggtcaa atcattttga ataaaatggg ggtggaaaca
    15361 ggaactgatc tttggaacga caaatactca ttagccggaa atacgtttgc cattcgcaaa
    15421 gacagtccaa ccggtgaaat tgtccaagaa atgaccacgg atgaaaaagg tcatgcggaa
    15481 acaccaaaag agattgccaa tgcgttggaa ttgggaacct attatgtgac cgaaaccaaa
    15541 gctagtaatg gctttgtgaa tacctttaaa ccagtaaaag tcgaattgaa ctatgtcaat
    15601 caaaccgtgg ctcttgttac cagtaacgta aaagggcaaa accaagaaat cactggtgaa
    15661 accactttga caaaagaaga caaagatacc ggtaatgaaa ctcaaggtaa agctgagttt
    15721 aaaggagctg aatatactct ctttactgca aaagatggtc aagcggtcaa atggagtgag
    15781 gcttttaaac cagagttagt gaagggaacg aaagcttctg atgaaacggt gactttgact
    15841 ttagatgaaa agaatcaagt tgctgttaaa cacttagcga ttaatgagta ttactggcaa
    15901 gaaaccaaag cacctgaagg atataccttg gatgaaacaa agtaccctgt ttccatcaaa
    15961 aaggtggatg ataacgaaaa aaatgccgtg attacccgag atgttacggc aaaagaacaa
    16021 gtcattcgct ttggctttga tttctttaaa tttgctggat cagctgctgg gactgccgaa
    16081 actggcttca atgatctgac ctttaaagta tcgccattgg aaggaaccag tgagattaca
    16141 ggtgccgaag atgaagcaat tacagcttat aacgagcaac taggttttga tggctatggc
    16201 aagtttgaaa atcttcctta tggggattat ttacttgaag aagtagaggc tccagaagga
    16261 tttcaaaaaa ttacgccatt agaaatccgt tctactttta aagaaaataa agcggattac
    16321 actaagagtg aatacatttt tacgattacc gaaaaaggac aaaaacaacc aattaaaacg
    16381 gtgaccgttc cttacgagaa actgacgaat aaagcatttt ctgttagctt gaatcgtttg
    16441 atgctctatg atttacctga agaagaagat agcttaactt ctcttgggac ttggaaagac
    16501 ggaggcaaag aattgacaga ccttgattct accgagctag ttgataaatt gagctataac
    16561 ttgcatgaaa tcaaagaaga ctggtatgtg gtagcgcaag ccattgatgt ggaagcgaca
    16621 aaagctgccc aagaaaaaga tgaaaaagcc aaaccagtgg taattgccga aacaaccgca
    16681 acgttggcga ataaagagaa aacaggaact tggaaaattt tgcataaatt aaccgcagaa
    16741 caagttttgg ataaaaccat cgtcttattc aactatgtgt atgaaaacaa gaaaccctat
    16801 gaagcaggtg atgatccagt agcgaaagat gctagcttga acaatcaagc ccaaactgtc
    16861 aattgtacgg tggaacgcca tgtttccatc caaacaaaag cccacctaga agatggttcg
    16921 caaaccttta ctcatgggga tgtggtggat atgttcgatg atgtgtcgat tacccatgat
    16981 gtactggatg gctcaaaaga agcttttgaa acaattctgt atgcattact tccggataat
    17041 acgaccaaag aaatttggaa atctggcaaa attgattatg aagtgaatga caaagaattc
    17101 actaaaaccg tacttgcgga aaaagtggac acaagtaaat atccagaagg tacaagtttt
    17161 accttcaaag aaattaacta tggcaaagac ggaaacatca atgggaaaca caatgaggat
    17221 ctaaaagaaa aaactcaaac tttaacacct aaaaaagttc cgacaacacc aagtgcacca
    17281 gaacaaccgg aaataccaac tgttccaagt gactctcaag aatctagtcc tacagtaaag
    17341 acattcccac aaacagggga gaaaaattct aatgttctgc tgtttattgg gtttaccttg
    17401 atctttgcga ctgctgggta ttatttctgg aatcgccgaa actaaggtga tgtgatgaaa
    17461 caactaaata ggaaaaacca acgtgcgcga ccgcctccta aaaaaagtgg tcgcgctatt
    17521 tttgtatcca aaactaaaaa tgaaaagagg aaaagaagat ggaattgaaa tttgttgtgc
    17581 cagatatggc cgaaaccttt ggaaagataa ggtatgccgg cgaaggcgaa gttttaactg
    17641 aaggatatgg gcggaatact acagtaattg gtcgtagtta tcatttgtat tccagtaagc
    17701 aacgagccga tgatattgaa gtggtggtag ctgcggaagc cggcgaaaag gattttgatc
    17761 aagatcaacc actaaaagcc gtgaatcccc atttggttgc caaaggctat gagattgaga
    17821 atcgtgggtt taccgactac gtgttgtatg tcgatgattt agtgaaagca taggaggaaa
    17881 agaagatgag attaacagaa ggtattgttg tagattcagg actaacgttt gggaaattac
    17941 gattttccgc attacgccga gaagtacgga agcaaaatga ggatggaacg gttagtaacg
    18001 aggtaaaaga acggacttat aatttgaaat cctctgcgca aggtcgaatg atccaagtta
    18061 gtatcccagc aaatgtaccc ttacgcgagt ttgcctatga cgcagaggta gagttggtca
    18121 atccaatcat ggatacggtc gccaattatg ttttccgaga aggaactact gtcaattggt
    18181 tcattaaggc cgatgattta gtcttgaaac gacaaccaga tcagggaaat tctacaaacc
    18241 aaaatgaagg aaagaaatag gtgaagcgta tggaaattaa tgttgaacat attttagatt
    18301 gtctcgatca atatggaaaa ggtgagctaa cggaggaaca actcactaaa gcattaacct
    18361 atgatgaaaa aatgtttctg attatgcatc aagggttact ggaagtaggt aacgatgtcg
    18421 aagaagattt tgatgtactg aattggttag gggaagaaga gtccttcttt atggttatcg
    18481 aagtaaatga aagtctatgt cgagaagccg aaaccgtatt ggaagaaatt ggtgtggaaa
    18541 tgccggatgc cattgaagtt tttttaaagc aactagtgga aacgaaacaa ctccctatag
    18601 cagtaaatga ctagcataac tgcttgaaat tggatcaatg aaatcctatg aagctttttt
    18661 cgattggagg tggaaaccat aaaaatgtac aaagggcatc gtatacgggc aggagatcag
    18721 cacttggttt atcactttgt tcttggctgg ctactcgctc tgttcatcgg ttggatgagc
    18781 gtcttttatt ttcaagagct tagacaattt gatatttcta agctatcact ttctactata
    18841 gaaatcgttc gttctataaa agatttaatc tatttgctag gaagtctcgt tctttcagga
    18901 agcacgatgt tgctttatat acactttttt caggatcatt ggcgcagttt gtggcatcga
    18961 caaaagctag cccggatgat tctagaaaat cattggtacg aagtaaaaca aactcagagt
    19021 gaaggatttt tcaaagacct caatagtagt cgaaccaaag agacaatcag ttacttcccc
    19081 aaaatctatt accgtatgaa ggatggttta ctttctattc gcgttcaaat ttcactggga
    19141 aaatatcaag atcagctttt aaagctggaa aagaagttag aaagtggtct atattgtgaa
    19201 ctagtagaaa aggaactcaa agactcctat gtggagtaca ctttactgta tgatataatt
    19261 gccaatcgaa ttgggataga cgaagtagtg gcagaaagtg gggctttgcg attgatgaaa
    19321 aatcaagtat gggcttatga ttctttacct catatgttaa tcgctggcgg cacaggtggt
    19381 gggaagactt actttttact cactatcatc gaggctttat taaaatctga tgccgagctt
    19441 tttgttcttg atcccaaaaa tgcggactta gccgatttag gtacggtaat gcctcatgtc
    19501 tattcccaaa aggaggaaat ttctgcttgt gtggaagatt tctatgagcg catgatgact
    19561 cgtagcaagg caatgaaaga aatgcccaac tacaaaacag gagagaatta tgcgtttctt
    19621 ggacttccac caaacttttt aatctttgat gaatacgtgg tttatatgga aatgttaacg
    19681 acgaaggaaa gtgcggtgat tttgaataag ctcaaacaaa tcgtgatgtt aggtcgtcaa
    19741 tctggtttct ttctgattct agcttgtcaa agaccggatg cgaaatattt aggagacggg
    19801 attcgcgatc aatttaattt ccgggtggct ttgggtcgta tgagtgaact gggatattcc
    19861 atgatgtttg gcgaggttga taaaaaattc tttatgaagc gtatcaaagg tcgaggctat
    19921 gtagatacgg gagggagtgt gattagtgag ttttatacgc cacttgtacc aaaaggatat
    19981 gatttcttaa gggaaattag cgacattggt ggattaaacg ttaatacgga aggataaaat
    20041 aatagtatgt agcctaggag tgtcaagaat cctgggcttt ttttacgagg tgatttatga
    20101 catttttaga tttattcgct gggattggtg gcttttgctt aggtatggaa caagcgggtc
    20161 accagtgtat cggcttttgt gaaattgatg attttgctcg ccagagttat aaggcaatcc
    20221 atgatacaag taaggaggtg gaaatgcatg acatcacaag tgtatcagac gagtttattc
    20281 aatctctcgg accagtggat atcctttgcg gtggatttcc gtgccaagct ttttcaattg
    20341 cgggaaagcg gcaaggattt tccgatactc gaggtacttt attctttgaa atcgctcggt
    20401 tcgccgctat tctccaacct aagtttttat tccttgagaa cgtccgggga ttactcaatc
    20461 acgaaggagg ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgatg
    20521 tggaatggca agtgcttaac tcaaaggcct acgttcccca aaaccgtgag cggatcttcc
    20581 ttatcggaca ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggatcgtctc
    20641 caacatctga tcaaaacatc agaaacttat taaatatcaa cccttccaat cgaggaatgg
    20701 ggggacaagt ctatgggtca gatgacgtag cgcctacctt gaccggtgat gaaggaatca
    20761 aaatcgcatt gccggtagat gatggaattt ctgtagcggg tatgttactg ggaaattttg
    20821 aacaaggcaa tcgggtttat gagataactg gaacagcgcc tactttgtca acgaaacaag
    20881 gcggaacgaa gatcatgctt cggaaaaacg aaacagaata tcaagaggtg aagccaggga
    20941 acagtgtgaa cctggctttt cctaattcca caagtcgtcg aggccgtctg ggaaaacaaa
    21001 gtgtgcatac cttattaaca ggagaccaac aagcagtcgt cacggatcag tatcaaattc
    21061 gaaagttaac acctagggag tgctggcggc ttcagggctt tccggactgg gcttttgatc
    21121 gagcttctca agtcaattca gatagtcagt tgtataagca ggctggaaat tctgtaacag
    21181 ttccggtcat ttttgatatt gctagaagat taaaggaggt aaaaacagat gatttatgat
    21241 gagttgattg gggaatctac tgggtgattg gcaagattca gtcagatccg gaattggaag
    21301 aagaactgta ccggctcaat tttgatattc gcaaaaacgg ggtgaaagtc cctgatgatc
    21361 cgtatgtaat tgatgaggaa acggatgctc gaatggagct gaatcaagtc atcagtgaat
    21421 tggaacgaat tgcggacttt gctaaagaac ctgatatccg ccaatacttg tttgagataa
    21481 aagcagagtt ggaaattgag ggtattaccg cggagtaacg gccaaataaa ccactgatag
    21541 gcactgaccg cgggggattt gcgaagcaga aatcctccgt ggtgctgtgc ctgtttatgg
    21601 acggcgttag ccggccatga tgtctcaccc cccgtatcta acaggggggt acaaaaacac
    21661 aaaaaataag caagcaacga taaggaatgg ctgtcctatc aaggataata agcaatttgt
    21721 gtagatgtta actgatttga aaaagttaac accttaaatt tggaggtgag aacttggcac
    21781 aaaggaattt agattatcgg ctattaaaag accggcgtaa tgaatatggc gtttcacaaa
    21841 ataaattagc tacgacttgt ggacttagtc gcccgtactt aaatcagata gaaaatggtg
    21901 gcgtaaccgc atcgacaaaa accatgagga aaatttttga ccaactggaa agtttcaatc
    21961 ccgagttacc tttaacgtta ctgtttgact atgtaaggat tcgctttccc acaacggatg
    22021 cgaggaaaat cattcaagag attctccatt tgaaatttga ttatatgctc cacgaagatt
    22081 acgcctttta ctcctatcaa gaacaatatg ttatgggaga tattgtggtg atgttgtcac
    22141 atgaagaaga taaaggcgtc cttttagaat taaagggtcg tggttgtcgg cagtttgaaa
    22201 cttttttact cgcccaaaag cgtagctggt atgacttttt cgaagattgt ctaaaagccg
    22261 gtggcgtgat gaaacgctta gacctggcaa tcaatgatcg agttggcctg ttagatattc
    22321 ctgatttaac gaaaaaatgt cagaaggaag aatgtatctc cttgtttcgt accttcaaaa
    22381 gctatcgttc cggggaactg ttgaaacctg atgataaaga tggtatgggc aatactttgt
    22441 atattggcag tcttaagagt gaggtctatt tctgtttgta cgaaaaagac tatgagcaat
    22501 atatcaagtt agggattcct ttagaccaga ccgaaacgaa aaatcgtttt gaaatccgac
    22561 tgaaaaatga tcgagcttat catgcaattc aagatttgtt aaaaggtcgc agtattgaaa
    22621 gtaccacatt ttccattatt aaccggtact tgcgatttgc ggataaggtg gaaggcaaaa
    22681 ggcgaactaa ctggcccttg aatgaacaat gggggcgctt cattggacgg aatcagaagg
    22741 aaattcaatt aacttctgaa ccgaagcctt ataccattga acgaacgttg aactggcttg
    22801 gacgacaagt cgcccctacg tggaagatgg ccaaggagtt ggatcggttg aatcagacaa
    22861 cgtacattca ggatatggta aataatactc gactatcaga ccggcacaaa aagattttgg
    22921 agcaacaaag tatggcaatt gaaaatttga ttatatgaaa agaggaatcg tgatggaaat
    22981 tgtgaaagat attttattgg tgtttggtgg cagtatgctt ggtgttactg tgatgtgctt
    23041 gatgcatgca agtgcagcgg cggaccgagc aatggaaaag aaaggaaagt gatgaaatga
    23101 atttcggtca aaattt
//
LOCUS       DAYYHX010000024        23112 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000024 DAYYHX010000000
VERSION     DAYYHX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.024"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACHA6Y_000695"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACHA6Y_000695"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM8986811.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACHA6Y_000696"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACHA6Y_000696"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM8986812.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACHA6Y_000697"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACHA6Y_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM8986813.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACHA6Y_000698"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACHA6Y_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM8986814.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACHA6Y_000699"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACHA6Y_000699"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM8986815.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACHA6Y_000700"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACHA6Y_000700"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM8986816.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="ACHA6Y_000701"
     CDS             complement(8054..8524)
                     /locus_tag="ACHA6Y_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM8986817.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="ACHA6Y_000702"
     CDS             complement(8570..8995)
                     /locus_tag="ACHA6Y_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM8986818.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="ACHA6Y_000703"
     CDS             complement(9062..9331)
                     /locus_tag="ACHA6Y_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM8986819.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="ACHA6Y_000704"
     CDS             complement(9342..10937)
                     /locus_tag="ACHA6Y_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8986820.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALCRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACHA6Y_000705"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACHA6Y_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM8986821.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACHA6Y_000706"
     CDS             complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACHA6Y_000706"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM8986822.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="ACHA6Y_000707"
     CDS             15403..16377
                     /locus_tag="ACHA6Y_000707"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8986823.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="ACHA6Y_000708"
     CDS             16547..17947
                     /locus_tag="ACHA6Y_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM8986824.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="ACHA6Y_000709"
     CDS             complement(18025..18747)
                     /locus_tag="ACHA6Y_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8986825.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="ACHA6Y_000710"
     CDS             complement(18826..19332)
                     /locus_tag="ACHA6Y_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8986826.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="ACHA6Y_000711"
     CDS             19455..21254
                     /locus_tag="ACHA6Y_000711"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM8986827.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="ACHA6Y_000712"
     CDS             complement(21322..22896)
                     /locus_tag="ACHA6Y_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM8986828.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6330 a         5018 c         4224 g         7540 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaat
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggca aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgacgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cg
//
LOCUS       DAYYHX010000025        22672 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000025 DAYYHX010000000
VERSION     DAYYHX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.025"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(200..520)
                     /locus_tag="ACHA6Y_000713"
     CDS             complement(200..520)
                     /locus_tag="ACHA6Y_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986829.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(1023..2141)
                     /locus_tag="ACHA6Y_000714"
     CDS             complement(1023..2141)
                     /locus_tag="ACHA6Y_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986830.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            2374..2496
                     /locus_tag="ACHA6Y_000715"
     CDS             2374..2496
                     /locus_tag="ACHA6Y_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986831.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            2612..3919
                     /locus_tag="ACHA6Y_000716"
     CDS             2612..3919
                     /locus_tag="ACHA6Y_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM8986832.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            4040..5239
                     /locus_tag="ACHA6Y_000717"
     CDS             4040..5239
                     /locus_tag="ACHA6Y_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8986833.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(5266..6534)
                     /locus_tag="ACHA6Y_000718"
     CDS             complement(5266..6534)
                     /locus_tag="ACHA6Y_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM8986834.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(6590..6656)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6704..7345)
                     /locus_tag="ACHA6Y_000719"
     CDS             complement(6704..7345)
                     /locus_tag="ACHA6Y_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM8986835.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(7754..8257)
                     /locus_tag="ACHA6Y_000720"
     CDS             complement(7754..8257)
                     /locus_tag="ACHA6Y_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8986836.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(8380..9144)
                     /locus_tag="ACHA6Y_000721"
     CDS             complement(8380..9144)
                     /locus_tag="ACHA6Y_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM8986837.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            9252..9527
                     /locus_tag="ACHA6Y_000722"
     CDS             9252..9527
                     /locus_tag="ACHA6Y_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM8986838.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            10087..11037
                     /gene="yidC"
                     /locus_tag="ACHA6Y_000723"
     CDS             10087..11037
                     /gene="yidC"
                     /locus_tag="ACHA6Y_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM8986839.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(11083..11484)
                     /locus_tag="ACHA6Y_000724"
     CDS             complement(11083..11484)
                     /locus_tag="ACHA6Y_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8986840.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(11498..12307)
                     /locus_tag="ACHA6Y_000725"
     CDS             complement(11498..12307)
                     /locus_tag="ACHA6Y_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8986841.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(12294..13184)
                     /locus_tag="ACHA6Y_000726"
     CDS             complement(12294..13184)
                     /locus_tag="ACHA6Y_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8986842.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(13197..13676)
                     /locus_tag="ACHA6Y_000727"
                     /pseudo
     CDS             complement(13197..13676)
                     /locus_tag="ACHA6Y_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(13852..14793)
                     /locus_tag="ACHA6Y_000728"
     CDS             complement(13852..14793)
                     /locus_tag="ACHA6Y_000728"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFM8986843.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(14790..15785)
                     /gene="lacD"
                     /locus_tag="ACHA6Y_000729"
     CDS             complement(14790..15785)
                     /gene="lacD"
                     /locus_tag="ACHA6Y_000729"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8986844.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(15778..16974)
                     /locus_tag="ACHA6Y_000730"
     CDS             complement(15778..16974)
                     /locus_tag="ACHA6Y_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8986845.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(16992..17720)
                     /locus_tag="ACHA6Y_000731"
     CDS             complement(16992..17720)
                     /locus_tag="ACHA6Y_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8986846.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(18161..19135)
                     /gene="lacD"
                     /locus_tag="ACHA6Y_000732"
     CDS             complement(18161..19135)
                     /gene="lacD"
                     /locus_tag="ACHA6Y_000732"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8986847.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(19132..19590)
                     /locus_tag="ACHA6Y_000733"
     CDS             complement(19132..19590)
                     /locus_tag="ACHA6Y_000733"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8986848.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(19587..21050)
                     /locus_tag="ACHA6Y_000734"
     CDS             complement(19587..21050)
                     /locus_tag="ACHA6Y_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8986849.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(21032..21964)
                     /gene="pfkB"
                     /locus_tag="ACHA6Y_000735"
     CDS             complement(21032..21964)
                     /gene="pfkB"
                     /locus_tag="ACHA6Y_000735"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8986850.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            22106..>22672
                     /locus_tag="ACHA6Y_000736"
     CDS             22106..>22672
                     /locus_tag="ACHA6Y_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8986851.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQ"
BASE COUNT         6809 a         4875 c         3815 g         7173 t
ORIGIN      
        1 ttgctctgat gttctttttt aaactaaaaa aattattctt tctgactact tatcggttct
       61 gtttcaacgg tcgaggctgg ggaagattct ttagtaggtc tgatgttctc atggtatttc
      121 tgatagccta tgacagttac cagaagaaca aggcaaacca atacgaccaa cgagattagt
      181 ttttcttttt tgctcatatc tatacctcat aaataacagg atgatgtcca tcatatttct
      241 gagtatgagt aaaagcaaaa gctgcagctg tagaatgtaa gaaatgccag caactttcat
      301 aatcacaggt cggacagtac caaaagtaac caaatcttgg ctgcaaagac cgttcttctg
      361 tttcaggaat cgcagaatca agatctggtg tctgtacata aacatttttg tacagacgtg
      421 tgcctgtaat aggaaaccgc tgtgtttctt cagcaaaaac tggctgactt gttaaactta
      481 gggatagaga cagcaaacac cataacagtc tttttttcat aaagacctcc ttatcgataa
      541 gtttttccca attaaaatca tatctcaaga acgcccgtta ttcacatttt gttcataaat
      601 gatgtttttt gtatcataat cgtcaaaaag aggtcgtgac cacgtcacaa cctctttttt
      661 aaacggtgtt ccagaagtag cttcttcgga aataagccga aatttcacaa aaatttgaga
      721 aataattttt gtcaatctct tcttatttct tgaagctgac cacttctgtc acaacctctt
      781 ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaccaaac aaaaaatatg
      841 aagagctatt tttgcttgct gttcttatta cttgatgcag aacagctttt tcacaacctc
      901 tcatacacgg tgtttaatca gctgaccttc ggtattagct caaaaaacgg caaaatatgt
      961 gaaactattt ttccgtttgt ttctctaata ctcaggtcaa aacactgatt tcacaacctc
     1021 ttttaataac gaagaatcgc tgtcagactc ttttcatctg gcgcatcttt ttgatccaaa
     1081 ataaacactt ttccgccatt aatgatcaca tcatctgcta atgcattcaa cacttgacgc
     1141 cgatcatatt ctgtttcagg atcttcaccg aaaccatcag ctaagtttga cgtagcgata
     1201 aataaatgtg acacttttcc ttctttcgca gctggaacga tatcaactaa ctgatcgatg
     1261 aatttcttat ctaagagttt attgtattct tttgattctt gagctgcgag ttcacttgac
     1321 agcttcttcg ccccttcttc gatatctcta aaggatagct gcgcaggaga agcagaaatc
     1381 gcaacgtctg cacaataata gggattttta gcaatttttt tgaacatcgt ctgattttct
     1441 ggtagagcaa ataaataaat cggccattgt tctggatttt cgaaagaatc cttcaagtac
     1501 gtatcaactg cttggtaata gttgacccaa tcgatctcta cttcttcatc ttttggattt
     1561 acgccatgga aagttactcc atctcgggct ccgcctcctc cattagaaga gaagttcaaa
     1621 ccgccacctg tcagttcaga tcccaatgca gtaacgacat caaccggtgc cccttctggt
     1681 agagatactt cttttacttg tttgttgctg acttcataca atttcatgga gtcgcggttc
     1741 aatcccaata aataataaga atagttgaac tgcccatttt tgataatagc cagtaaataa
     1801 ggaagatcac ttacatagta ttgatcgtct accgggatac tcaaacgatg gatataggtt
     1861 tggtcagcag ttataatgac cgctacactc gtagttgcac tacgccaaaa cgaaggatct
     1921 cctaacaaat tttcaatttt ttcttggaac ggggaccagt catgttcgga atattttttc
     1981 tcaaaacgtt tctttgcttc tttcgcaaaa tttttgaatt tgatctgatc tttttccaca
     2041 tcttgatggg cgacatgcgt attcagcatg atcgtgacga atggtccttt agcgccttct
     2101 gaagtcaatt gtgacaataa ttctttatcc ttttttgcca taaacgtacc cctcctaaaa
     2161 aaatcattcc tttgtttcta tctaaagtct accacaacac tatttttcca tacaataaaa
     2221 cgtatctaaa aaaataaaac ggtttctacg gttttaaatt tagctatttt tttaagttat
     2281 cttcataatt tcctcaatta tttagcccca gtcttcaact attcttcatg aaatgctgat
     2341 aaactaagaa aaagaataaa agaggtggat cttatgcatt tgattatgat tttcagttta
     2401 ttaagtatta tcagtttatt tgctttcagc gcttcccgct tattagatgc ggaaacacag
     2461 cctattcctg taaaagtacg taaaaacgac cgctaacctt ccataacgtg cgttcttatc
     2521 ctaaagtttt ttcagaaaca ttgaaactct ctcccctctc tgataaaatt ttaatttgta
     2581 gcgaaacaaa ttaattacaa ggagatcgga tatgtccata aaaaaaacac gtctagtcct
     2641 attattggct agtctcattc tttgccttgc ttcttttcta ccagctgttg ctgtgcaagc
     2701 tgaagatact ttcaaagtaa atgcaaaagc agcctttgct gtagatgcgc aaagcggtaa
     2761 gattctttat gatcaagatg gagaaaaaac catggggatc gcttcaatca caaaaatcat
     2821 cggtctttac atcgtccttg atcaagtaaa agaagggaaa ctatcatggg atgataaagt
     2881 ttcaatttca gattatgcag aaaatttgag tgtcacccca gatttatcaa atgttccttt
     2941 gcataaagaa aacacctaca ccgttaaaga attattcgac tctgcaatca tccagtcagc
     3001 caacgcttca atggttgcct tggcagaaaa aatctctggc agcgaagcaa aattcactga
     3061 acgaatgaaa gaacaattga aagattgggg tatcaaagat gcaacgatcg ttaatgcatc
     3121 aggactgaat aactcttatc ttggcgaaaa ccgaccagaa ggtactggag aaaacgacga
     3181 aaatcaaatg tctgctcaag atgtggctat cgttgcccgt catttgatct tggattttcc
     3241 agaaatactg gatgtatcta gcactacgac acaaatgttt ggcgaaaaca cgcaatcacc
     3301 agtagaaatg gtcaactgga actggatgct cccaggattc gtgaattata aagaaggcgt
     3361 ggacggatta aaaactggta cgactgaact tgcaggagcc tgtttcgtcg gtacgatcac
     3421 taaaaacgat caacgaatca tcactgtcgt attgaatgca gacggccatg cggaaaaccc
     3481 aagtgcccgt ttcaatgaaa cagctcgctt aatggattat tgctacgaca attggtcaga
     3541 aaaagagctt ggaaaagcca atgcaagtat ccctagtcta aaaaccattg atgtaaaaga
     3601 cggaaaagaa tcaacagtgc ccgttgtgct taaaagtcct gtaaaagtat gggtacggaa
     3661 cgatatggat actggaaaat taacaatcac accaactctt gacaaaaaac aagtcactga
     3721 caatgagatc caagcaccta tggataaagg aacaaaaatc ggtaccgcaa cgatcacctt
     3781 ggcagaagat aaattaggtt atcttgaaga caacaacgcg ccatccacag aaatcatcac
     3841 ggataaaacc gtagaaaaag caaatatttt cgttattggc tggcgacgcg tcgcagaatt
     3901 tttcggtaac ttgttttaat tgaacaaaaa gaaaaaagca tctttccatg tggaagatgt
     3961 ttttttgctt tttctagttt aaataacagc ttttttcatc tattttcgtt atactgtata
     4021 agaaaaagga gttgaattta tgaatctaac cagagatctg atttttttgg ttctctatct
     4081 tattggggta ttctccatcg tttattggcg aaaacagaaa aaggatcctt ccttgttctt
     4141 ttacagcagt acatggacgt ttgcggtaat cttgacttat tttgtcgtgt tggaaatttt
     4201 ttattcgacc ttttattttt tgattccctt agtgtttttc agtatctttg ccttttccta
     4261 tttttcagaa aagcgaaggc tgttgaatgg tcttttattc aatattttcc tcattagttt
     4321 tggtatctac ttgtttgtct tgctatacga aacacaaaat atttttcttg gcggactgat
     4381 tgccttgatc acgatcccgt tactgcttgt ctttctattc ggcatttatg gattgatcgt
     4441 ttttcttttt tggaacggtg tcacagtttt acgtagagag tcgcattcat tagctaatct
     4501 tttaacgttg atactagcca tttttcttac attgtttctt gtttttgact ttttcttgtt
     4561 aaaatattta ccacaatgga taaatgcttt gtttttctgc atcccattga tcttgatcta
     4621 tctatttatc gtattctaca actttttgac tgtctcattc ttgtatcagt tcaaccgccc
     4681 tcgatacaac caagacttca tcgttgtgtt aggcgccggg ttgatcaatg gagaaacagt
     4741 ttctcctctt cttgccaagc gaatcaataa ggcgattgct ttttattggg cacaaagccg
     4801 tgcgacatta aatccgccta tcctattaat gtcgggtgga cagggagcag acgaaaaagt
     4861 accagaagca atggcgatga aacaatatgc catggaacaa ggaattcctg aaagagatat
     4921 tcttgtcgaa accaattcga caaccacttt ggagaatatg cgctattcaa aggaaatcat
     4981 ggatcagcaa atgaaaggtc cctatcgcgc gatcttcagt tccaacaatt accacatttt
     5041 cagagctggt ctctatgcac gacaagcgaa actgaaggca aatggcattg gggcaaaaac
     5101 agctttctat tacttgccta acgcgttttt aagagaatac atcgccatct tgatgatgaa
     5161 caaaaaaaga catatgatca tttgcggatt gcttttcgca ggttccgcat tcttatcact
     5221 gatcacttta cttttctaaa aaaacaaggt gtgacaaaac atatgtcaca ccttgttttt
     5281 ttcttttatt ctcttgacga ttggcaagaa tagtattcct tttttgacgg ctacttgctg
     5341 tgcagaataa ataccgctgt ttcctgaaca cataaagctg atcaagcaga tcataaagaa
     5401 gtaagcagca gcttgtgaac caaataattc gattcccatc acaaaacagg caatcggtgt
     5461 atttgtcgca ccagaaaata ccccgataaa acctaatcca gcaagaaaag gaacagaaac
     5521 atggagcaaa ggcgctagta ctgcgccaag cgttgcacca atctcaaata gtggtgtcac
     5581 ttctccccct tggtaccccg cacctaaaga caagaccgtg aagaataatt tcccaataaa
     5641 atcataagca tgtgcctgtc cattaaatga ttcctgcagt aacggaagac tcaacccaag
     5701 gtaacgttgt gtttgcaaaa tcaacgctgc ggctacaact actgctccac ctatcgtatt
     5761 tcgccaaaca ggatgaggaa tccattgcgc ataccgcgct tttaaaaaga cgatcgaacg
     5821 gctgaatacc caaccaatca aaccaaaagc aatcccagca acaaaaactt tgaagaacag
     5881 caacatactc cattcaggaa tcacacccat ttggtaatgt agatgagaaa caccatagct
     5941 ttctgtcatg aaatttgcaa aaagcgcagc aaagaagcta ggaaatattg cttctgttcg
     6001 gactttaccg attgccaaca cttctaatcc aaataatgtt ccagctaaag gtgtgccaaa
     6061 tacagaacta aaacccgcac tgattccact gataaccaaa atctcccgtt ctgctttatc
     6121 caatcggaat aaatgcgcta tattatcagc taatgctccc cccatttgaa ctgctgttcc
     6181 ttcacgacca acagatcccc caaataaatg ggtagtgatg gtcccaaaca aggtcaatgg
     6241 aatgagccgc aaagggatct tctcttctcc tccattccct tgatcgatca ctaagttatt
     6301 cccacgacta gaaagaccgc catgatacgc ataaagataa gcaaaaagcg cgccgctgac
     6361 tggcaataga taaagtagcc aaggatgtgc tagtcggata tctgtcacga acgtcaggct
     6421 atttaagaaa aaagcagaca agctccccat gaatactccg agaatcgctg tgaccaccag
     6481 ccacttcaac atgtacatca aaatacgcac tggactcggt aattgttttt ccataaaaaa
     6541 ttcctcctca aataaaaaaa cgcctactaa agaaaggggc tggccccaga ctttagtagg
     6601 cgtcattagc tatcttacgt tagcatttaa aggcgaacac catcacctat tcacttccat
     6661 taaaagatag catatttttc tgtaaaaaac aataaaatta tcgttatttc tttacacgtt
     6721 cagcaaattc atagaaagga gccaatttcg ttaactgttg attacattga tcactatcat
     6781 gacaaatata attccccttt ttcgtatacg tgccatcaga accggctttc gttgtagaaa
     6841 gaaagagcga gacattcgat gttttctgac agatcgcaca gattccctta ttaatcgttg
     6901 gtgataaagt accgctgatc ccttttaatt ttccatcttc gtaataaacc atgaattttt
     6961 tttgcgtccc tggatcattc caaccgatgt acgtatgtgc ttgcaagtcg atttcattcc
     7021 aagcgggttg tttgagtttt ttcacttttc ggaataattt ctcgatttgt ggtttgctcg
     7081 gctgcataaa cggctggacg atttgcttca gctcctccaa tgctcgttct gcttgcgttt
     7141 ttgtcagttt tacatctaaa actgtcgtta aaaacgtatc gatttctgat ggttcgtaac
     7201 ctaatacatc gcgaatcttt tcagctgcta gagcctgaac cgtttgtacc gtttttgagt
     7261 cattgacaga tgagtacgca tttaataact gctctacttc tctttttata aaaaaatatt
     7321 gatatggttt cattgtttgt tccatgattt taaagtcctt tcttctaacc aaagtgcata
     7381 ctttgtagaa aaaagtgatc ttctgcaaag tatgtgttac ctgaagatcc cggactcttt
     7441 taaataagat acgttttgca tcttatcatc acccccttta atgttcattc ttctatgtac
     7501 atgattcatt cgataaataa aagtaaaccg tatcttgagc agatagtcaa ctgcaaacag
     7561 aaaaaggact gtgacaaaag tcgtgtcaaa gtccctttct acaaacaaac ggtgttccag
     7621 aagtagcttc ttcaaaaaat aagccggaat ttcacaaaaa tttgagaagc aattttcgta
     7681 aattccttct tatttcttga agctgaccca cttctgtcac aacctcaata tggtctatta
     7741 ttggttttac ctgttaaatc gaggattctt ttccgaagta acgttgccat ctcttacgca
     7801 tacttgctga cataggctga gcggcttctt tgactgcgca gtatttgtct acgaatgtta
     7861 cgatatatcc ttctttgtac ttaggcggtg caatcgttgc gccccacatg tgcttcaaaa
     7921 tgatatctcg ctcaagatcc gacaaatcgg tgatcttttc tgcattcttt acagcgattc
     7981 ttggatggat atacgcatgt gtgccttcat caaatttcgt cgtacgccaa tcatagtaga
     8041 acagatcatg caaaagtcca gcacgcgctg ttgctttagc attgccaccc catttttttg
     8101 cgagcagata actatagtaa gaaacactga tggaatgctc caaacgagtg gaatgaacat
     8161 gctgtgtgta attcgctaat tttttcactg cttctgtttc taaaagatct tctacataag
     8221 acatatattc ttcgtcttca cgccattttt ctgtcataat tatcgatctg ccaccttttt
     8281 gaatttcatt ttcttctgaa attgatctaa gtataccacc agtgttcgga ttattctagt
     8341 cgccttggat aaaatagcca aagcttaata gttccttaat cataaagaaa aatgataaag
     8401 aacaatccct gccgcaaccc caacattcaa cgattccgca tttcctttca tcgggatata
     8461 tacgttctga tcagtcaaag acaagatttc ctgacttacc ccctgacctt cgttccccat
     8521 gattattgcg aagttatctg atttttctac tttgtctaag gcgtgtgctt cttcatttaa
     8581 ttctgtgcca tacactgtaa accctttgtc tttcattaaa ggaatcactt ctgtcaaatc
     8641 tttttgcaac acaggcaaat gataattgct tccctgcatg ctgcgcagca cctttgtact
     8701 ataaatatct gccgttcctt tgcctaataa aacaccagcc atatcaaatg catctgccgt
     8761 acggatcatc gtccctacat ttccaggatc ttgtacatta tctaataaca gccatcctcc
     8821 tgaaaaatct ggcgtgcctt cagccgttaa cgccatcact gctaagattc cttgtggtgt
     8881 aggtagatcc gatacagcat ccatgacctc atccgaaact agaaaagtag aaatctcttt
     8941 gtttgcggcc agccagtctc cccattcttt catgcctcgc tgtgtcacaa aaagttcttt
     9001 tatttctgca tcagacgacg cagcttcttc aatcagatgg aacccttcca aaagatattc
     9061 ttccttttct tcccgatatt tctttttatg tagttttttt aattctttga ttcttggatt
     9121 ttttgctgat tggatctctt tcacattcct cacctcccca tgattatatc atggaatagg
     9181 tgcttgagct tgtttcatcc taaaaaaatc ggtatcatta atttataaaa taggaaagaa
     9241 aggtgacgaa catgcgcaaa gttaaaatga acgtccaagg acgcgtccaa ggcgtaggat
     9301 ttcgctacat gaccaagatg gtcgctgatc aactaggcgt gactggaacg gtaaaaaacg
     9361 aagatgacgg ctcagtcacg atcgaagcaa tcggcaatga cgacatcatc cagaaattta
     9421 ttgaagaagt caagaaatct ccgagcccaa gtggacgagt gcagtatgtg gatgtccaag
     9481 aagacccgat gattgaggaa cgaaagaaat ttgatgtggt gggataaggt tgtgaaaaag
     9541 ctggtctgca tcaagcatta aggacaggtt gtgaaaaata gctctccata tttttcaaca
     9601 ggctggactt aatgccgtag atgcaagctt ttgaacaccg tcaataaggt tgtgagaaag
     9661 tcgcttagtt ttgagtagta aggagaattt tgtgaaaatg gctttccaca tttttgcata
     9721 aaatttgact taataccgag aaactgactt ttgaacaccg tcgataaggt tgtttttgag
     9781 acgctttgcc tcaagtatta aagataggta gtaaaaaagc tgatctataa cttgcaataa
     9841 atttgcttaa cccatatttt ccagacagaa caaaaaagtt tttagttttt taaataagaa
     9901 agaaacactt ggaatcactc ttttctttcc aagtatttct ttcttgattt tttgtctttt
     9961 tttcaaaatt tttcgctacg taaaagaacg aatatttata tgaaatttct atgaagttga
    10021 ttgaattcca tgcgcttttt tagtatagtt aagtttgtgt aaaaattata gaagaggaag
    10081 tattctatga ataaatggaa aaactggtta ttgggatccg gacttgtcag tatcctgctc
    10141 tttttatctg gctgtgtgcg gatggattca aacggaaacc ctgacacttc gggaatcgtt
    10201 tatcgagtac tcgtccatcc tatgggccaa gcgattacat atctagttga gaatttcaat
    10261 tggtcttatg gctgggcagt tattgcaatg actgtgatcg tccgtatcat catcttgcca
    10321 ctaggtatta gtcaatcgaa aaaaacaatg atccaatctg aaaaaatgca agcgttaaaa
    10381 ccacaagttg aagcagccca acaaaaattg aaagctgcaa caacacgtga agaacaaatg
    10441 gcagcacaag cagaaatgca gcaagtatac cgcgaaaacg gattgagtat gaccggcgga
    10501 atcggctgtc tgcctctatt gatccaaatg ccgatcttct ctgccttgta ctttacggca
    10561 cgttatacag aaggtattcg ggaatcatct ttctacggca tcgatttagg ctcaccaagt
    10621 atggtacttg ttgtcattgc aggggtcgcc tatctgcttc aaggatacat ctcaacgatc
    10681 ggtattcctg aagaacagaa aaagacgatg cgtacgatgc taatcgtctc tcctgctatg
    10741 atcgtcttca tgtcgatcag tgcgccagcc ggtgttacac tttactgggt agtcggtggt
    10801 gtgttcagct gtctgcaaac attcatcaca aacgttatga tgaaaccacg tttgaaagca
    10861 caagtggcag aagagttgaa acaaaacccg ccaaaacaag tcgtcactcc aagaaaagac
    10921 gtgacagaat cagcaaagcc agctaacaac aaacaagcaa caaaacaaat caaacaacca
    10981 aaaaacagta caaacaacaa aggccgcaac gcaggaaagc agcagaagcg gaaataaaaa
    11041 cataggacaa aaaagatgag agctaagccc tcatcttttt tgttactctt cctcttctgc
    11101 tattccatta attaattcgt tgacagagac aatgctttca catcctgcac gaacaaactc
    11161 gggctctgaa ccaatcattt gtgagttgat aaattcaatg accatcggaa gattcatccc
    11221 agcataaaga gtaatctgtt gtccttccat tattagttta gaaatgatat ttgcaggtgt
    11281 accacctaaa agatcgcata gtaccacaaa atcatttaac gaatctgtga cagtgagaaa
    11341 cttttgtttg tatgtttcag tgccatcttc tggtaataaa ggtaacgtgt agatatcctc
    11401 ttgaggcccc atgatcattt ctgtactttt tttcaattcc tcacaaaatt ttccgtgact
    11461 aatcaatacc aattgtctac tcatttttcg ctctccctta tttcgaaatc acacccaatg
    11521 cagataaagc aacacacaca attaatacga taaaaatcgc tcgagtagga gtcatgtttt
    11581 tctttcctaa taaccaataa acaccaccga cgatcccagc aggtaaaatt ttaggtaaga
    11641 tcatatcgat atagttttgc atattcaatg tcacatcgcc aatcgttggt gcccatgaaa
    11701 atttaacatt gatcattgtc gcaactaatg cccctaccat gaatacccct aataaagtgg
    11761 ctgcgtccgt taatgcagtt aagcgagatt gcatagtagt aactaaggaa attccttctt
    11821 tgtatgcaaa ttcaagttgc ttccaacgaa aaaccatgac aacgatctga gcaaaaatcc
    11881 atataaacac acctatcgga tttccatcag ttgccatatt cgccgccaaa gccccaaaaa
    11941 tcgttggaat caaagcagca aatacggaat ctccaatcgc tgcaaaggaa cccatcaatc
    12001 cagctttcag ccccgatacg gtttcctttg aagcaattcc ctctttttct tccattgcaa
    12061 gatcaatccc agtaatgatt gtattaaaaa aattagaagt attaaaaaat tgcgtatgtg
    12121 ttttcatcat ctcttttaat tctggtgtat tgtctccata tatttttctt agttgtggca
    12181 agatagtata taaatagcca gaaccctgca ttctctcata gttccaacct aattgaaaag
    12241 tgaacaaact tcttcgattg atttgcttaa aatcttcttt tgttaatttg taattagatt
    12301 tcgtcatctt cgatttcccc ctcaacagaa tgtgatggct cactagctaa tggttgatgg
    12361 tctttgtttt gatcattctt ataactgatc attgctagtg ctagaccaat caatgctaca
    12421 gcaagcatcg gcagcgaatt gaaagttgcc ggaagatctt taacaacgct tgccacactt
    12481 gttcctaata attgaacatt agagaataac gttgctaata atgccgtgat cgtaaatcct
    12541 aaaataagat aagatagatg tttttttact ggtaaatagc gcagaagaat ggcaaaccca
    12601 acagctggta aaacagctcc tgctactgaa agtccatcgc ctagccattt tagattcccg
    12661 tttaaaacag aaacaacatt ttcaactaat ccaccaccaa atgctaaagc taaaaataca
    12721 ggaatcatac gagagagcga ccaaggtaaa gctcccatta agaaattgcg ttcgattgct
    12781 tgataattca tgtcttctac gtgacggtca atacgatggg caaagtatgt gttagcgaat
    12841 ctagctaaaa tatccaattg gatcattaag cttgctacag gcactgcaat agcagcaatc
    12901 gcttgttctg gattcattcc aagagaaatc gagaaggcag ttgctaaaat cgtcccagag
    12961 ttggcatcta tttttgatgc gcctccaaac gtccccactc ctaaaactgt caactgcata
    13021 ctgccaccaa taatcaaacc tgcgtttaaa tcacccatta ctaaacctgc atataaccct
    13081 gcaaacactg gcgcactcat tgaagaatag atctgtaatt catctaaaat ttgataacct
    13141 gcatataatg ttaataataa gatctgccac cattgaatat ccatggatca ttcctcctat
    13201 ttgattaaaa gattcataaa gtcttctgct ttatcgtttg gtaccatttg agcaattaaa
    13261 tgtataccaa gctgatctaa ttttttaaat acttcgatat ctgcatctac cacattgatt
    13321 gattttgtaa tggaccgtgt ttcattcgtt tgtgacatat tccccacatt tacttctttg
    13381 ataggaactc ctttttctac taaagataag attcgatctg gcttttttgc tacgatcaat
    13441 actcgttgag agtcatattt gccagctaaa atatttgttg ctgctttttc gattggcaaa
    13501 atgcttaatt ttacccctgc tggagtcgct aacttcaatc cacttttttc aattgtgttt
    13561 gtggctacct catcatccac gaccatgatt cgactaatat tcagtttcgt tgtccaaaga
    13621 tttgctactt atccatgtat caaacgtcca tcgattctta caccaataat tgccatcatg
    13681 cattctcctt atctattttt tctatttatt gttcaattaa aactgtgaca tcatcaaata
    13741 tcccttctgt ttcaaagata acgttttcat tttttctttc ttatatttca tttttaagct
    13801 aagaacaaat tccttcccaa ctaaagaaaa cgctttcttt tatatttaag ttcatttgat
    13861 tttcttaact tgaatgtgtt tcatcaacat cgtaactttc atagggtcga tccagccagt
    13921 tttctcttgt tgcgcgtttg ccataccaca agccatagaa aatgcgagaa tctcttcgat
    13981 tgattgcctt tttgataacg catgggccga tcctgcaagg gtcgcgtcac ctgaaccaac
    14041 aggattaaca actgatattt taggaataat gacttcgtaa aaacactgag caacttttgc
    14101 cacagcacct ctctctccta aagaaacaac aacccatggc actccttcaa atattggatg
    14161 agctaataag gactgaattt tttctaattg aatcgaagaa cagatacgtt ggccaatcag
    14221 ttctcctaat tcttccagat tgggcttgat aagtagtggc ttctccttac cttccaatac
    14281 ttcaagcaaa aatttaccag aagtatctag taatatgtct ttcccttttt tatcagctaa
    14341 agcaaccaat tgcttatagt aatcaagagg gcatccttgg ggtaaactac ctgagatcgt
    14401 cataatatct gatatttcga ggagctgctc gaaatgatcg gtaaatgtct gttgttcttt
    14461 ctttgttatg aaggggcctc gttccaaaag ctctgtttgt ctcgaactat gcaggatagc
    14521 aatagaatcc cttgtctctc ctttaattgg aacaaactga tgcatgatcc ctaactgatc
    14581 tagctggttc tttagatatt caccgtgctg gccacctatt aaaccagtag ctatcacctc
    14641 ttctcctaat aaatttagaa ccctcgccac attcactcct tttcctcccg ctgttttatt
    14701 gactgtttca actcgattga ctttgtcgag gtttaattct tcaattgtat acgcgatatc
    14761 gatggaagga ttcaaggtaa ctgtcgtaat catttcatct tcaccttttt gctttttgtt
    14821 ttattgaacc aaggggtagc ggtttcattt attactttat ttaaagtctc gatgttttct
    14881 ctcccatttg ttcttaacca atgaatagct ccgtcttttc cattttcgac gtatgctttg
    14941 accccatctt tccatgtcgc acgtccacac aatacaccat tgaaggtgga acccgattca
    15001 tgagcaaaaa tcagtgtttg ttgaaataat tctgttgaaa cacccccact caaaaaaatg
    15061 aatggtaatg gagtcgcttc actttgctcc ttaaagaagg cagcagcctc ttctttgctg
    15121 tgaataaatt cacctgtgga atagccattc acataattca tattgactgg tacttctact
    15181 ttcaacacat caactttata ttttggatca gaaaattctt tcatcatctc aataactttg
    15241 tggggcttga ctttcgcata ctctggagaa gaactatcgc ttatttgtgc gtcataagaa
    15301 actaactcca aatagaaggg acgattttct ccttcgcact cactacctaa acgttcgatg
    15361 aacacatgct tcaaatgatt gatttcgggt gcttcatcaa catcgtagta aagtaagaat
    15421 tttactgcat ccgcttgctg ttctttcaat ctgagagaag accaatccgc taataaatca
    15481 ggtagacgac caggcgtagt tgcgtcatag ccggttttct cataagctaa caataacccc
    15541 gtctcttttg ctctgacttt tgctgctggc aatccatatt ccggatccaa taaaatagca
    15601 gaagcatagg gtgtcagctc agctgatact agttctttga attcactgat ttggtcttca
    15661 cttggctctt ttcccaacga tttgatcatt ttttttagag caccacgctg atcgatcgct
    15721 aatgcactaa taatcccatc ttttgttgat agtctctcca ttgccctatt tttttcttca
    15781 ttcatctgtc atttctcctt cggtttcata cttatgaata atgacgccct tgactactcg
    15841 attgaccgtg cctgttggag atggtgtatc tggtttatta gctactttga ttgaagtata
    15901 gagtgcaatg atttgtgcag tcataatcat cgctaatgca agataggcat cgggcaattt
    15961 actcaccgag tcttccaata gcacagtcat acctgagaaa tcaatagtac ccggttgcgc
    16021 aatcgcaatg acattggcag tgacccgatc ttcttttatt tcctctaaaa tatctaagtc
    16081 ataatttcgc gtatatggtt gattgctcac aaaacctaca acgagtgtct cgctatttaa
    16141 aaaagacttt ggtccatgac gaaaccccat cgaagaatca aaaattgtag caactttacc
    16201 tgctgttaat tctaaaattt ttaattgtgc ttctcttgtc gcacctgcca aacttcctga
    16261 acccagataa gcgatccgtt caaaaaaatc aggtaataac ttttgaattt cttgatctct
    16321 tgaaagaaga tcttgaccga ttttaactaa ttgttcaaga tatctttctt ttttctccat
    16381 tgctgtttga tcaaagatcc ataaagcact caataacata cacgtaaaac ttcctgtcat
    16441 agcaaaacct gcatcgtttg ctctctctgg catcaataac agataattct gtttatcatc
    16501 ctgtgcgttt tttgccaaca tcccctcttt agcacaagta atcgtcaaat gaaacaatgt
    16561 ttcaacttgt tcatttgcaa tttgcacagc cgctacactt tctggactgt tgccactgcg
    16621 agcaaatgaa actaataaag tcggttcatt tggaaacaaa aattcttttg gtgaagctac
    16681 aatatctgtc gtgccaaaac tgtggaagat aaaaaactga ttgtctccat gttcatttag
    16741 atagggtaag agcgtatccc caatatattg agacgttcct gcacccgtaa aaataacacg
    16801 tattcttctt ccgtttgctt ctttttttat ttgatcaaaa aatagagcta aattttcttg
    16861 gctttgcatc aatctatcga ttgtttcttt ccagagatct ggttgttgtt ttatttcttg
    16921 tgtcgtgatt gctgcaccta tttgttttaa tttgttcttc gatagctcaa acatgttgtt
    16981 ctccctctca atcattttcg gatatgccga attttataat gaaactggtc cgctcttgct
    17041 acactatctg tatattcgat aatctcattt ttcacgttat aggttatcct cgtcagatgt
    17101 aagacgggag cgccatcagc gattcgtagc acaacagcat cttttttttg cacgatactt
    17161 gcataaaact cttcatcagc taattgaact tgttgttcat aatcctgtga aaacacatca
    17221 tacaacggtt tactttcaag taacggcaaa tcaagtccca taaatagttt cgccggtaga
    17281 taagaacgct caaccatcaa tggctcttca tctgccaaac gcaatctttt aagtttaaat
    17341 acagtttcac ctaaatggat gttcaaattc ttagcaattc tcttattagc ttccatttgt
    17401 ctaaattcta aaatgaccgt cttagggacg cgccctattt cgcgcatttc ttctgtaaag
    17461 ctatatgctc cagctaagtt cgatgactta tcagacaaat cagagacata ggtacctttt
    17521 ccatgtcttc ggtaaatata accttgattt tccaattcct gtaaagccag acgtacggtg
    17581 gtacgactca ccccatagtg ttgacaaagt tctctttcag ataacagctt atcatgcgga
    17641 gtcatctgtg tttcgatacg atccttcaat aaatcaacta gttgataata caatgatttt
    17701 cctttatttg agctctccat aaaaatcacc tcttctttac tggttataac cacaacacaa
    17761 gaatagtgaa ttattaatca tttgtcaaag gttttttaga ccaaaaaagc tattataaca
    17821 gctctttaaa gctttatatc ttgaatagac aaagatacct caaaaatacc agttaccaat
    17881 aaaagaacag aaaaaaagac gaaccacata agcgatccgt caaaatggtc taacttatcg
    17941 agcagcgtga tccacattaa tccctatgat attcctacaa ctatcgtttt attacttcca
    18001 atatcattta caacaactaa aaatagctta ttaatgacaa atgttattat tagttttaga
    18061 taacttttgc agttttatac tttttttcta taaaatttga caaacatttt tttaaataac
    18121 taattatttt ttgaatctgt cactcagaca atcctttctc ttacttgtta acataaatag
    18181 aagttgctgt ttcttctaat agtgtactta attctttaat attttgtttt ccttcttcta
    18241 acatccaact actcgctttt tcttcgcctt cggtaacaaa aggtgctact cccttcgccc
    18301 atgttgctcg cccacataag acaccattga atgtcgaacc tgattgtttt gcaaaacgca
    18361 atgtatcttg aaatagtgca gcgctgactc ccgcacttaa aaaaataaac ggttgttcag
    18421 tcactttcga ttgttcatag aaatacgcgg atgcctctgc ttgactataa caagtatctt
    18481 gcccatatcc ttccacaaat ctcatattca ctggtacttc tactttcaat acatccactt
    18541 gataacgttc ttttgaaaat tctcgcatgg cttcaatcac tttatgcggt tttacttttg
    18601 catagtcaag agaagacgga tcgccaatag ttgcatcgta agaaaggagc tctaaaaaga
    18661 aaggtatgcc ttctgcttcg cattctgaac caatccgttc tataactatt tgtttttgca
    18721 cattaatctc tactggttca tcaacgtcat agtacaataa aaatttacaa gcatccgcac
    18781 ccacttcttt taaccttttc actgaccaac tattcagtaa atctggtagt cggcctggca
    18841 ttttcgcatc atagccggtt ttttcataag ctagcaacaa accacattct ttctctcgaa
    18901 gcgctgctgc aggcagacca tattctggat cgagtaaaat agcggaagca tacggagtca
    18961 ggtactgaga aaccatctgt ttaaattgga tgatatcttc tgatgtagct tctttatatt
    19021 ggttgatcat tttcttcaaa gcaccacgtt ggtcgatagc tagcgcgcta ataattccag
    19081 cttcatccgt caatcgcttc aaacgttcct gtttttctat actcattttc ttcatatatc
    19141 ttcacctatt tctttagtta atattgtagc tatttctatt tttgattttg cagctctcaa
    19201 ctgttctgta acctcttttc gcatcaatga tcgtgcaacg agagacaata atttcaaatg
    19261 agtcgttcct gcttctgaat ctgggataaa taacgcaaaa ataaaatcaa ctgactgtct
    19321 atctaaactg ttccattcaa ttcctttttt cagtgtgacg ataatcaaac taggttcatt
    19381 gattgtttga gacttcgcat gagggatagc aaaacctgac attattcctg tagtaccttc
    19441 tttttctctt tccagaagtt tttgatatac ttctttttcg tcatgagcga ttcctaaaca
    19501 gaatgtttgc tttgctaaaa acttaaatgc ttcttcttga ttatctaatg gctgttgtaa
    19561 aaaaatgttc tcttcatgaa tgattgtcat gataaacctc ctgctcttcc ttcttcgata
    19621 actgtacgat ttgttgcttt attatccctc ttcttgtctt tatcagttac tcttttcttt
    19681 tggatcaaac ctagtatcac tccaccaacg atcgaaccaa taagaatcgc tgtaacccat
    19741 tggatttttc ccgtcactac aggtaacact aaaaaaccgc catgtggggc tggtacttgg
    19801 acaccaaaca gataggtcaa gacagcagca attgatgagc ctaacatcat cgttggtaat
    19861 actcggatcg ggtctttcgc agcaaacgga atagctcctt ccgtaatatg agttgatcct
    19921 aaaataaaat taaccaaacc tgcattacga tcattagtat caaaatattt accaaacaat
    19981 aaagtagcaa aaccagtaat caacggtggt gcaatgcaag ctgctgacgc accagccata
    20041 aaagtcgtgt ttccttgtgt caaaagagct gttcctgtta catatgccgc cttattaacc
    20101 ggtcctccca tatcaaacgc acacatacag ccaacaatca cgccaagaac aagtggattc
    20161 gaatattcaa atccagctaa aaagctcatc attccttcat ttatagcctt cattggacct
    20221 gccagcaaag acatcacgat gccaataaat gctacaccaa taactggata taagacgatt
    20281 gccttcaagc catctagttg tttaggtaaa attatcagta gcttttgcaa aaataagata
    20341 aaataacctg ctaagaaccc agctaaaata cctcctaaaa atccagtacc gccattcatg
    20401 gctacgattc caccaacaaa tccaacgact aaccctggac gtttcgcgat cccttctgca
    20461 atataagctg ctaaaactgg aaccatcatt cccattgctg catcgtcaac tgcttttacc
    20521 attgccgctg tagcattgta ttgtgcatga tctggatcag aagaatagat tccccaaacg
    20581 gcaaatgaaa tagcaatcaa tactccgcca gctaccacga aaggcaacat atgagagaca
    20641 ccgttcatca aatttttata gatgctacga ccaaaattag caccacttat cgcttctggt
    20701 tctaaagtat cagcagcttc attatctgct aagacagtac cttttcctgc caatgcttcg
    20761 tcaatcagcc gttgtgcatc cttgattcct gtactgacag aaacatcaat gactcgtttt
    20821 cccacaaatc tttctgaatg gacatctttg tctgcggcaa taatgacagc atctgcctgt
    20881 ttgatttctt cagccgttaa ttcattctcg atcccaattt gtccatgggt ctccactttg
    20941 atcgtgatgc cttttttcct agctgcttgc tctaatgcct cttgcgccat gtaggtgtgc
    21001 gcaattcctg tcggacaacc ggttgctgca attagttgat attttcccat ctttcgaacc
    21061 tctcttcctt gattttgatt tgtttactta attcctccac atccaaaaaa tcagtcagtc
    21121 ctttacgaaa agcagtagag ctgcctgctg ctatgctttt acgtaacgtt tcatcaagcg
    21181 atcgtcgatt catgtatcct gctaaaaaag tgcctaacat ggcatcacct gcacatgctg
    21241 tattgacaac ttttcctttt ggcgagtttc ctcgaatcac cctgtctttc gtaaaaagaa
    21301 tcgctccttt ctcaccgagg gataacaaaa cattttctgc gcctgactct actagtcgtt
    21361 gtccataaac aaagtaatct tcatctgtaa ccatttttcg tccgaaccaa gaagaaagtt
    21421 cttcctcatt tggtttcagc aaaaacggtc gatagggtaa acaatccatg acttcttgat
    21481 cactactatc aataatcaaa caaatccgac tgatttctat taaaaccttt ggagacagcc
    21541 ctcttggtaa actcccagaa acacaaagat aatcccctgc ttgtaatgct cttatttgat
    21601 ttaaaaaatt atgaacagct gtctgcggta tttcaggtcc ttgattcact aatttgtatt
    21661 cttctcccgt ttgattgact tgtgtaaata cattgatacg cgtcatacca ggcacttcaa
    21721 taaactctgt ttggatttgt ttctgctgta aatagtcttc tatatacttt cctgtgaacc
    21781 cagcagaaaa acctaatgcc gtacttggga aatctagcat ctttaatact agtgacacat
    21841 tcaccccttt tccgtttgcc tgaatgtcat cctcttttgt gcggtttacc ataaaaggat
    21901 gcatctcatc tgtttcaata aatagatcaa ttgccaaatt catcgtgcat gtgtagatag
    21961 acataaccaa cgacctcctt gattctatta tgaaatacgc gaaatagaaa atgttttctt
    22021 aatcgtccca acacaaagaa aacctttaca tttataaact cgtcgtgctt tttttaacta
    22081 acttttggtg aaagaaggaa agaatatgaa tcacaaatta agtgaaacag agcagtacct
    22141 ctggaacttt atcgaacacc tcatcctaga gatccctaac tattccatcg tgaaactaag
    22201 tgaacaagca aatgtctcaa cagccaccat tgtgcggaca atgaaaaaga aaggctatga
    22261 agggtttact tcgtttaagc atcatctaaa agaaggagaa aacaagaaca tcaatttttc
    22321 atttttagat aaagtagaca aaggaatccg ccgagccatt ctaaaaaatg aacaagaagt
    22381 ggtacgtaca atcaatatgt tggaaatcgg gaacattgaa gatgcaatac aaagaatcaa
    22441 atttgctgac cgggtttttg tctttgcccg aggattttca gaaatgattg gccaagaaat
    22501 gctggtaaaa cttcaattaa cgggaaaaaa ttgtcagatg catactgatc cagaaattat
    22561 caaaagcatc agtcggaaac taactaagaa agacagcgtt atttttgttt ctttgaacgg
    22621 tgagaaaaca aataaaaaca tcaaaaataa gatttatgtc tttatttttc aa
//
LOCUS       DAYYHX010000026        22642 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000026 DAYYHX010000000
VERSION     DAYYHX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.026"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..851
                     /locus_tag="ACHA6Y_000737"
     CDS             39..851
                     /locus_tag="ACHA6Y_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM8986852.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            848..1648
                     /locus_tag="ACHA6Y_000738"
     CDS             848..1648
                     /locus_tag="ACHA6Y_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM8986853.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1809..3077
                     /gene="rseP"
                     /locus_tag="ACHA6Y_000739"
     CDS             1809..3077
                     /gene="rseP"
                     /locus_tag="ACHA6Y_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM8986854.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3145..4854
                     /locus_tag="ACHA6Y_000740"
     CDS             3145..4854
                     /locus_tag="ACHA6Y_000740"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM8986855.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5066..9424
                     /locus_tag="ACHA6Y_000741"
     CDS             5066..9424
                     /locus_tag="ACHA6Y_000741"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM8986856.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9569..10042
                     /gene="rimP"
                     /locus_tag="ACHA6Y_000742"
     CDS             9569..10042
                     /gene="rimP"
                     /locus_tag="ACHA6Y_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM8986857.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10065..11240
                     /gene="nusA"
                     /locus_tag="ACHA6Y_000743"
     CDS             10065..11240
                     /gene="nusA"
                     /locus_tag="ACHA6Y_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM8986858.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11262..11555
                     /locus_tag="ACHA6Y_000744"
     CDS             11262..11555
                     /locus_tag="ACHA6Y_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM8986859.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11552..11863
                     /locus_tag="ACHA6Y_000745"
     CDS             11552..11863
                     /locus_tag="ACHA6Y_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM8986860.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11876..14182
                     /gene="infB"
                     /locus_tag="ACHA6Y_000746"
     CDS             11876..14182
                     /gene="infB"
                     /locus_tag="ACHA6Y_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM8986861.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14209..14556
                     /gene="rbfA"
                     /locus_tag="ACHA6Y_000747"
     CDS             14209..14556
                     /gene="rbfA"
                     /locus_tag="ACHA6Y_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM8986862.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14713..15169)
                     /locus_tag="ACHA6Y_000748"
                     /pseudo
     CDS             complement(14713..15169)
                     /locus_tag="ACHA6Y_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            15334..17696
                     /locus_tag="ACHA6Y_000749"
                     /pseudo
     CDS             15334..17696
                     /locus_tag="ACHA6Y_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            18064..18987
                     /gene="truB"
                     /locus_tag="ACHA6Y_000750"
     CDS             18064..18987
                     /gene="truB"
                     /locus_tag="ACHA6Y_000750"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM8986863.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18991..19932
                     /gene="ribF"
                     /locus_tag="ACHA6Y_000751"
     CDS             18991..19932
                     /gene="ribF"
                     /locus_tag="ACHA6Y_000751"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM8986864.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20284..21069
                     /locus_tag="ACHA6Y_000752"
     CDS             20284..21069
                     /locus_tag="ACHA6Y_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8986865.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21082..21933
                     /locus_tag="ACHA6Y_000753"
     CDS             21082..21933
                     /locus_tag="ACHA6Y_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8986866.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22057..22509)
                     /locus_tag="ACHA6Y_000754"
     CDS             complement(22057..22509)
                     /locus_tag="ACHA6Y_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8986867.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7683 a         3954 c         4953 g         6052 t
ORIGIN      
        1 aagatagtgg gaatctttta agtataggag gaaaaagaat gttacgtttt tttccgcaaa
       61 aaaacaaata tattcaagaa aaaagcgaat ttcaatttaa taccgatgga ccaattcctc
      121 agcacattgc catcatcatg gacggcaatg gtcgttgggc gcaaaaccgt cgtttgccac
      181 gagttgctgg ccataaagaa ggcatggaaa cagtaaagaa agtcacaaaa aaagcatcaa
      241 ggcttggagt gaaagtgttg acgctttatg ccttttcgac tgaaaactgg aagcgaccaa
      301 aagatgaagt aagttttctc atgcaactac ctgtcgactt ctttgatacg ttcgtaccag
      361 aattgattaa agaaaatgtc aaagttcatg tcatgggtta tgaaaacgta ttgccagaac
      421 atacgcaaga tgctgtaaga cgtgcaattg agcaaacaaa aaacaatact ggaatggtat
      481 tgaattttgc tttgaattat ggaagcagag cagagatcgt gactgctgtc caagaaattg
      541 ccgaagaagt agcaaaagga gaaatacacg cagaagaaat cgatgatgaa ttgattgcta
      601 agcatttaat gacaggtttc ttgcctaaag agttacaaga tcctgagctg atgatccgta
      661 caagtggaga agagcgaatt agtaattttc tgttatggca gatcgcatat agtgagctgt
      721 attttacaaa agcgctttgg cctgattttg atggtgcgca tcttgaagaa gcaatcgcct
      781 cttatcaaaa tcgagaccgt cgattcggcg gtgtaaaaaa aacagaagat gagaaagggg
      841 atcagtcatg aaacaacgtg tggtcacagc agccgtggcg ctagtcttgt ttattccgat
      901 tatatggatc ggaggcatag cggttgagct tacagcagca atcttagcag taataggagt
      961 ttacgagctg tttcggatga aaggattgac actcttaagt tttgaaggag ttttatccgc
     1021 cgttggtgca gtgttacttg ttttgccaaa agaaagatgg tttttctttc ttccagagaa
     1081 tgtaagtaca tttatgttat tttatcttat agttatgatt cttttaggaa catcagttat
     1141 ttctaaaaat acgtatacga ttgatgaagc aggttttcct gtgcttgtca gcttgtatgt
     1201 gggaataggt ttccagaatt ttgtcaatgc acgaacagaa agcttggctg ttttgatatt
     1261 tggtctattt attgtttggg caactgatat cggagcatat atggttggtc gacgttatgg
     1321 tcagcataag ttaagtcctg atatttcgcc caacaaaacg attgaaggtg cgctcggtgg
     1381 tattggtagt gctgttgtag tcgcatttct ttatttattg ctatacccag caaaagaact
     1441 atttaatcat ggtatgtttg tcatgcttct gatgacgatt attttttcag cagtcggaca
     1501 atttggtgat ttggtcgaat ctgcaatcaa acgtcattat gatgtgaaag actcgggaaa
     1561 tatcttacca ggccatggtg gaatcttaga ccgtttcgac agtttgctgt ttgtatttcc
     1621 tatcatgcat ttatttggca ttatttagta agtatacctg cgtttctttg tgaatttcat
     1681 aaaagacgca ggtttttatt tttcattata ttgatactat tggtgtcttt ggtatactat
     1741 ggtaaaatta tagtgtttta tcgttgtttt ataaaaaaca caaaaaacaa gtaaaggaga
     1801 aaaaagctat gaaaacgatt ctgacattta tcatcgtttt tggtatatta gtgattgttc
     1861 atgagtttgg tcatttcttc tttgcgaaac gatcaggaat cctcgttcgc gaatttgcta
     1921 tcggtatggg tccgaaaatt tatgggcatc aagcaaaaga cgggacaaca tatacgcttc
     1981 gcctattacc tattggcggt tatgttcgaa tggcaggcaa tggggatgat gaaacagaaa
     2041 tggcaccagg aatgcctctt tctcttttac taaattctga cggcatcgta gagaagataa
     2101 atttaagtaa gaaaatccaa ttgacaaatg ctatcccgat ggaattgagc cgttatgatt
     2161 tagaagatga gctgactatc actggttatg tgaatggaga cgaaacagaa gtagtaacct
     2221 accctgttga tcacgatgcg acgattattg aaaacgacgg aacagaaatc agaattgcgc
     2281 caaaagacgt acagttccaa tcggctaaat tgtggcagcg tatgctgacc aactttgctg
     2341 gacccatgaa taactttatt ttggcaatcc tgttattcat cattctggca tttatgcaag
     2401 gtggtgttca agtgacgaac actaatcgcg taggagagat catgcctaat ggagctgcgg
     2461 cagaagcagg cttaaaagaa aatgatgagg tagtcagtgt ggacggaaaa gaaatccatt
     2521 catggaatga tctgacaaca gtcattacga aaaatccgga taaaacatta gactttaaga
     2581 tcgaacgaga aggtcaggtg caatctgtcg acgtgacacc gaaatctgta gagtcaaatg
     2641 gcgaaaaagt tggccaactt ggtataaaag ccccaatgaa tacaggtttt atggataaga
     2701 tcattggagg gacgcgtcaa gctttcagcg gttcattgga aattttcaaa gccttaggat
     2761 cactatttac aggtttcagt ttagataaat taggcggacc tgtcatgatg taccaattgt
     2821 cttcagaagc agcaaatcaa ggtgtaacaa ctgttattag tttgatggcg cttctttcaa
     2881 tgaatctcgg aatcgtcaat ctgcttccga ttcctgcctt agatggcggg aaattagtct
     2941 tgaatatttt tgaagggatt cgcgggaaac cattaagtca agaaaaagaa ggaatattga
     3001 cattagctgg ctttggattt ttaatgttgt tgatggtgct agttacatgg aacgatattc
     3061 aacgattctt tttctagaaa aagttgacaa atagggtata ataacaaacg ataactattt
     3121 tgaggaaata aaggagtata tagaatgaga caatcaaaaa tgttgattcc aacattacgc
     3181 gaagttccaa atgatgcaga agtattaagc catcaaatct tgctaagagc tggatatatc
     3241 cgccaagtat cagcgggtat ttattcatat ctgccattag caaatcgtgt attggaaaaa
     3301 ctgaaacgca ttatgcggga agaatttgaa aaaatcggag cggtagaaat gctgatgcct
     3361 gccatcttgc cagcagaact ttgggaagaa tccgggcgtt acgaaacata tggaccaaac
     3421 ttgtatcgtt tcaaagaccg caatgatcga aaaatgatcc ttggaccaac tcacgaagaa
     3481 acttttactg atttgatgcg taatgaaatc aattcatata agaaattacc tttaaatctt
     3541 tatcaaatcc aacctaaata tcgtgacgaa aaacgacctc gcttcggttt gcttcgcgga
     3601 agagagttta tcatgaaaga tgcgtattct ttccatgcaa cggaagaaag tttggacgag
     3661 acatataaag attacgaaaa agcctacacc gaagtattca agcgttgtgg cttagaattt
     3721 cgctcgatta tcggagacgg tggcgcaatg ggcgggaaag actcaaaaga attcatggcg
     3781 atctcagaaa tcggggagga tacgatctgt tattcgactg aaagtgatta tgctgcaaac
     3841 ttggagatgg ctacaagcta ctatgtatca aaaaaatcgc atgaaacaca gcttgaattg
     3901 gagaaagtag ctacacctaa cgtaaaatca attgacgaag tagcagattt cttttctgtt
     3961 gaacctcaaa aaatcatcaa gtctgtattg ttcattgcgg atgaacaacc tgtccttgtt
     4021 ttggtacgcg gagaccatga agtcaatgac gtaaaactga aaaacttttt atctgctgac
     4081 tttttagaag aagcaacaga agaagaagca caaaaatatc taggtgctga gtttggttct
     4141 gtcggaccag tcggtgtttc agatgacgta aaagtatatg cagaccgtta tgtccaagat
     4201 ttagcgaatg ccattactgg agcgaacgaa acaggattcc actataaaaa tgtgaatcca
     4261 gaacgcgatt tcactgtact ttcttatgaa gatcttcgtt ttgtacaaga aggagatcct
     4321 tcaccagaca acaatggtgt tcttgccttt actcgaggaa tcgaaatcgg acatatcttc
     4381 aaaatcggca ctcgttacag tgaatctatg ggagcaactg tactagacga aaatggacga
     4441 gaaaaatttg tgattatggg ctgttatggt atcggtgtaa gtcgtttatt atcagcaatc
     4501 gtagaacaac attcagatga acaaggaatc aactggccaa aaggaattgc gccatttgat
     4561 ttgcatatcg tccaaatgaa tctaaaagac gaataccaaa caaacctgac gaatgaacta
     4621 gaagaagcga tgacaaaagc tggctatgaa gtactggttg atgacagaaa tgaacgcgca
     4681 ggagtgaaat tcgcggattc tgatttgatt ggctgtccaa tcagaatcac tgtcggtaaa
     4741 aaagcagtgg atggaatcgt agaagtgaaa atcaagaaaa ctggcgaaat ggttgaagta
     4801 cgtaaagaag aattggtgaa tactttacca atcctcttga accaaggcga ataataagcg
     4861 ttaaaacaca gaaaataaaa atagctgaat cagctgtttt tgacaatctg atgattcgct
     4921 tttaaaattg gaactgcgat ttgaatcgca gttccaattt tttaatgtaa aactcagaaa
     4981 aaacgagtat actaaaaggt actgaaaaag aagaggataa gatgcgatca attgcattgt
     5041 ttctcatcag aaagggcgta tatctttgtc agaaaaacga gatttatttg aaaaactgtt
     5101 ggaacaaatc caactagagg aagcagaaaa aaatcatcca ctgcttactg ctggtgaaat
     5161 ggatagtgta gtggtccatc gtaaatcgcg tttatgggaa tttacgctgc atttttccaa
     5221 aattttgcct attatgctat atcggtcatt gactcagcat ctgactatcg cttttaaaga
     5281 tattgcacaa gtcaaactga atatcttagc agatgaccag acattcgatg aacagctctt
     5341 gcaagattac tgggcccaag ctttggaaaa tcagcaatgc gatacaccac tagcacagca
     5401 agtattgaaa acgcaggtac ctgtgataaa agatcgtaaa gtcatcttgc caatcgacag
     5461 tacaggagct atttcttatt tgaagcaaca atacttgcca ttagtagaag agttgttcgt
     5521 cagttacgga ttcccaaaat ttcgtatcga accagaagtg gatgagcagc aagcggaacg
     5581 agtattgaaa ctttttgaag aacgcaaaca agaacaggca gaagccttta tgaagcaagc
     5641 agctgaaagt ctagttgtcc acgaacaaaa gaaaaaggaa agaaaagagc aatcgccagc
     5701 tcttgaagga cccatccaca tccagctagg acgaaatatt ccagccgatg aaccaacgac
     5761 accaatgatc agtatcgttg aagaagaacg aagagtcaca cttgaggggt acgtattcga
     5821 caaagaagtc agagaactac gttcaaaacg caagatcttg actttgaaaa tcacagatta
     5881 tacttcatca tttatcgtca aaaaattctc gaacggcgaa aaagatgaac aagtatttga
     5941 tgcgatcagt gtcggtagct ggttaaaggt acgaggaagt attcaagaag atacgtttgt
     6001 ccgtgatttg gtcatgaatg cgcaagatat catagaagtc aaacatacac ctcgcaaaga
     6061 ttatgctcct gaaggggaaa aacgagtaga gcttcatgtt catagcaata tgagtacgat
     6121 ggatgcaaca aacagcatct ctgatcttgt ggcacaagca gggaaatggg gacaccgagc
     6181 tatcgccatt acagaccacg gtggtgcgca agcctttccg gaagcacata gtgctggaaa
     6241 aaaagcaggt gtgaaaatct tatacggcgt ggaagcgaat gtcgtagatg atggtgtccc
     6301 aattgcttat aatgatgcac atgaagcatt gagtgaagcc acatatgtcg tatttgacgt
     6361 ggaaacgaca ggattgtctg cagtatatga cacgatcatc gagctagccg cagtcaaaat
     6421 gtataaagga aatgtgatcg aaagctttga tgaatttatc gatccaggac atcctttgtc
     6481 acgaacaacg gtagatctga ctggtatcac ggatggaatg gttcgaggat caaagtcgga
     6541 agaagaagta ttgcggatgt tccttgaatt ttcaaaagat acaattttgg tagcccacaa
     6601 cgcggctttt gatatggggt tcttgaatac tagctacgca aggtatggta ttcctgaagc
     6661 ggccaatcca gtcattgata cgttggaatt agctagatat ctgtatcctc aattcaaacg
     6721 tttcggatta ggtgtgttat ctaaaaaatt tggtgtcagc ttggaacagc atcaccgagc
     6781 aatctatgat gctgaagcaa ctggtcactt agcatggatc tttgtgaaag aggcaatgga
     6841 caatcataac atgctttatc atgatcagct aaatgagcat atcggagaag gagattctta
     6901 caaaagggct cgacctttcc atgtaacgat tttagcaaaa aatcaggcag gactgaaaga
     6961 tttgttcaaa ctgatttcaa tgtcgaatgt cgaatacttc gaacgcgtcc cacgaatacc
     7021 acgctcccaa ttgaaaaaga tgcgtgaaaa tctattgatc ggctcagctt gtgataaggg
     7081 agaaatattc gaagccatga tgcaaaaagg ggtagaagag gcaagaaacc gagcaaaatt
     7141 ctacgattat attgaagtta tgccaaaagc ggtctatgcg cctttgatcg agcaagaatt
     7201 ggtaaaaaat gaacatgatt tggaagaaat tatccaaaat ttggtagaaa tcggaaaaag
     7261 tttggataaa atcgttgttg caacaggtaa tgtacattat ctgaacgaag aagatgccat
     7321 ttaccgaaaa attttgatca attctatggg cggagcgaat ccgttgaatc gtcatagttt
     7381 gccggatgtc catttccgaa caaccgatga gatgctaact gcatttcact ttttagggga
     7441 agaaacagcg aaagagatcg tagtagaaaa tacgaataaa atcgcagata tctgcgaaga
     7501 agtgattcca gtcaaagatg aactttatac accgaagatt ccaggttcag aagatgaaat
     7561 atcagaattg agttacacaa aagcaaaaca aatgtacgga gatccgttac ctgagatcat
     7621 tcaaaaacga ttaaaaaaag agttgaattc tattaatggt aatggcttct cggttattta
     7681 tctgatcgcc caaaaactag tgcataaaag taatgaagat ggctatctag taggttcacg
     7741 tggctctgtt ggctctagct ttgttgcgac gatgacagga atcactgaag ttaatccgtt
     7801 agcccctcat tattattgtc cagagtgcca atattccgag ttttttgaag atggtacgta
     7861 tggttctgga tttgacatgc cagaaaaaca atgtccaaaa tgtggtgcac gcttaaacaa
     7921 agacggtcat gatattccat ttgagacatt tttaggattc catggagaca aagtgcccga
     7981 tattgatttg aacttctcag gtgattatca agcagaagcc cataactata cgaaagtatt
     8041 gtttggcgaa gactatgttt accgtgccgg aacgattggt acagtcgccg acaaaacagc
     8101 atatggctat gtaaaaggat acgaaaggga caataatctg cagtttcgaa gtgcagaagt
     8161 cgacagatta gcaaaaggcg ctactggagt caaacggaca accggccagc atccaggggg
     8221 gatcatcgtt attcccgatt atatggatgt ctatgatttt acgccgatcc aatatccagc
     8281 agacgatcaa aattcggaat ggaaaacgac tcattttgat tttcactcga ttcacgataa
     8341 tgtattgaaa ttagatattc tagggcatga tgacccaact gttattcgga tgcttcaaga
     8401 tttatcaggg atcgatccac aaacgatccc tacggatgac ccggaagtga tgcggatatt
     8461 tgccggtcct gaagtacttg gagtaagtca ggaacaaatt tattccaaaa cagggacatt
     8521 aggtattcca gaattcggca ctcgttttgt tcgagggatg ctagaagaaa cgcatcctac
     8581 aacttttgca gaactactgc aaatttccgg actatctcat ggtacagacg tatggttagg
     8641 aaatgcggaa gaattgattc gacgaggaga tgcgacactg gcggaagtaa tcggctgtcg
     8701 tgatgatatt atggtttatt tgatccatgc tggattagat agcgggatgg catttaaaat
     8761 catggaaact gttcgtaaag gacaatggaa caagatccct gatgaattgc gagaaaccta
     8821 tctatcggcg atgaaagaaa ataatgtgcc cgactggtat atcgattctt gttcaaaaat
     8881 caaatatatg tttccgaaag cccatgctgc agcttacgta ttgatggctc ttcgtgtcgc
     8941 ttatttcaag gtttatttcc ctattttgta ttactgtgca tacttttctg tacgtgcaga
     9001 cgactttgat ctagtctcca tgtgcaaagg aaaagatgct gtgaaacaag caatgaaaga
     9061 aatcacggac aaaggattag atgcttccgt aaaagaaaag aatcaactga cagtgctgga
     9121 gttagccaat gaaatgctgg aacgaggatt caaatttgga atgattgatt tgtataaatc
     9181 ggatgccgtt aactttgtta ttgaaggtga tacgttgatc gcaccattta gagctgtccc
     9241 aagtttggga accaacgtgg caaaacaaat cgttgaagca agaaaagacg gtccattttt
     9301 atccaaagaa gatcttgcca cccgcggtaa agtttcaaaa acattgatcg aatatatgaa
     9361 cgacaatggc gtattgaaag atctaccaga tgaaaatcaa ttatcgttat tcgatatgct
     9421 ataagcatct gttttcctgt ctgcttgtaa ttttaagcag agtatggtat agttcttata
     9481 ggtaatgata ctaactgtga gtgagcggat ttttcgctca ctctttttaa tggaattacg
     9541 cacttaattt tttaaggagg cgaaactttt gagtagcgtc gttgaaacag tcacagaaat
     9601 ggtgacaccg attttagatg aacaaaattt tgaacttgtc gaagtcgaat ttgtaaaaga
     9661 aggaaaaagc tggttcttac gagtatttat tgacaaagaa ggcggtatcg atatcgaaga
     9721 atgcgccttt gtcagtgaga aactgagtga aaaacttgat gcagcagatc cagatccgat
     9781 tcctcaagct tatttcctgg aggtatcctc cccgggagca gagcggccat tgaaaaaaga
     9841 aagcgactat gaaaaggcac ttggtgatta tgtccatgtc tccttatacc aaccagtaga
     9901 tggcgaaaaa caatatgaag gatttcttca atcatttgat gctgaacagc ttacattgaa
     9961 aatacgcata aaaacacgag aaaaagaatt gacttttgac cgaaaaaata ttgcaaaagc
    10021 tcgcttagct atccaatttt aatcacggag aggaatcaga aaaaatgagt aaagaaatgt
    10081 taaatgcgtt agacgcttta gaagctgaaa aagggatctc aaaagaaatc gtgatcgatg
    10141 ctttagaagc agcattggtt tctgcataca aacgtcacta tggccaagca caaaacgtcg
    10201 aagtggaatt tgatcagaaa aaaggaaaga tccatgtcta tgcggtaaaa gaagtaacgg
    10261 aagaagtgat ggattcacag ttggaagtct cattgaaaga tgcactttta atcaatccag
    10321 catatgagat cggtgatacg atccgttttg aagtaacacc aaaagatttt gggcggatcg
    10381 ctgcgcaaac ggctaaacaa gttattttac agcgtgtacg tgaagcagaa agaacgatta
    10441 tttataacga attcagtgct tatgaaaaag acatcatgca aggaatcgtc gaacgccagg
    10501 ataaacgtta tatttatgtc aatcttggta aaattgaggc agttctttct aaacaagatc
    10561 aaatgccaaa tgaattttat caaccacatg atcgaatcaa agtttatgtt tcccgagtag
    10621 aaaatacgtc taaaggtccg caagtatttg ttagccgcag ccatccagac ctattgcgcc
    10681 gcttatttga gcaagaagta ccagaagtct atgatggaat cgttgaaatt gtcagcatcg
    10741 cccgtgaagc tggcgatcgt tcaaaagtgg cagttcgctc aaatgatcca aatatcgatg
    10801 cagtcggtac atgtgtcggg ccaaagggac aacgagttca agcaatcgtc aatgaattaa
    10861 aaggcgaaaa catggatatc gtcgaatgga atgaagatcc agctgtattc attgcaaatg
    10921 cattgaatcc tgcacaagtc atggatgtga tcttcgatga gacaaatcca aaggcctgca
    10981 cagttgtcgt tccagactac caattatcgc tagcaatcgg taaacgcgga caaaacgcac
    11041 gtttagctgc taaattgact aaccataaaa tcgatatcaa atcagaatct gacatgacgg
    11101 aattttatga aaatcaagaa caacaaaaag aaacagaaga actgcatgat gaagcaatcg
    11161 ttcagtcaga tttaacagac gatgaatatg aaacaattgc attcaacaat gaaacggtcg
    11221 aagaaaaacc agaagcttaa ctttagtaag gaggcaaaga gatgaaacaa aggaaaattc
    11281 ctttacgcaa atccgtggtt tcaggtgaaa tgaaacctaa aaaagagatg gtccgaatca
    11341 cccgttcaaa agaaggggaa gtatcgatcg atccaactgg aaaaatgcct ggacgaggag
    11401 cgtatgtctc acttgagccg gaagaagtac aacaagcttg ggataaaaaa attctcgatc
    11461 gagtactcga aacaacatta acagatgagt tttatcaaga acttcttgac tatgtcactc
    11521 accaaaaagc acgtaaagag ttatttggaa aatgaataca gttaatcggc aaaaagccat
    11581 gaatctcatc ggtctagcga tgcgcgcggg aaaactaatc acgggtgaag aactgaccat
    11641 tgctgaaatc cgcaaacaaa aagcaaaatt ggtgtttgtg gcaaccgatg caagcgagaa
    11701 tacaagaaaa aagataaaag ataagagttc atattatgaa gttccttgct ttgagctatt
    11761 ttctgaagca gaaatcacgc agatgatcgg aaaacctcgc aaggtattcg gaataatgga
    11821 tgccggattt gcgaagaaga ccaaggagct aattgaaggt taggaaggtg attgcatggg
    11881 caacaaaaga atctatgaat tagcaaaaga actcaaaaaa tctagtaaag acgtcgtgga
    11941 aaaagcacaa caacttggca tcgatgtgaa aaaccatatg ggtgcgatta ctagcaatga
    12001 tgagaagaaa ttgcaacaag cgttcaaaaa cccaagcacg aaacaacaag ctgcacaaca
    12061 agcaagcaga aaaccagcca atcaacagcc gaacgatcaa cgtaaaaaag aaacaacgaa
    12121 caatcaacaa aaaaataaaa ataaccgcag ttatcaagat cgcggtcaag ggagcggtca
    12181 agtgaatcaa ggaaaaaaac caacaacaaa ccaaaataat acgggaaata ccagcagcca
    12241 aaatcgtcaa ggaacgagcc aaggcggcaa tcaaaaccgt actaacaata acaacaaacc
    12301 aaacaataac aatcgcggca aatttaataa taataaccgt aatcgttata ataaaaaagg
    12361 taaaaaaggc aaacagcaac aatcaactaa accagctgta ccaccacgca aattccgtga
    12421 attgccagat gtattagagt atactgaagg tatgaacgta gcagatattg cgaaaaaaat
    12481 tcatcgtgag ccagcagaaa tcattaaaaa attatttatg cttggtgtca tggtcaatca
    12541 aaaccaagca ctggataaag atacgattga actgttagcg acggactatg gcatggaacc
    12601 acaagaaaaa gtccaagttg acattgctga tatcgataaa ttcttcgagg cagaagaagt
    12661 cgatccagat aaattagtct cacgtccgcc agtcgtaacg attatgggac acgtagacca
    12721 tgggaaaaca actttattgg atacattacg ccattcacgt gtaacaagcg gcgaagccgg
    12781 cggaatcaca cagcatatcg gtgcctacca gatcgatatt gatggtaaac caatcacttt
    12841 cttggataca ccaggacatg cggcctttac aagtatgcgt gcacgtggag caagtatcac
    12901 agatatcacg atcttagttg ttgctgcaga tgatggcgtc atgccacaaa cagttgaagc
    12961 aatcaaccat gccaaagctg caggcgttcc aatcatcgtc gctgtaaata aaattgataa
    13021 accaggtgca aaccctcaac atgttatgca agaattaagt gagtatgagt taattcctga
    13081 agcatggggt ggagaaacga tctttgttga gatctcagcg aaattcggcc aaaacatcga
    13141 agaattgctg gaaatgatcc tgttagtcgc agaagtagaa gatttaaaag cagatccaac
    13201 acaacgcgca atcggaacag tgatcgaagc acgtcttgat aaaggtaaag gtcctgtgtc
    13261 aaccttactt gtccaagaag gaacacttca tgttggtgac ccaatcgttg tcggtaatac
    13321 ttacggacgt gtccgtgtaa tgacaaacga tcttggccgt cgcgataaag aagcaggacc
    13381 tgcaacgcca gtagaaatca ctggcttgaa tgatgtacct caagcaggtg accgtttcgt
    13441 agtcttcgaa gacgaaaaga cagcacgtgc agcgggtgaa gaacgcgcga aacgtgcatt
    13501 attagaaaac cgtgctgcca caagccgtgt gactttggat aacttatttg aaagcctgaa
    13561 agaaggcgaa ttgaaagaag ttaacgtaat catcaaagca gatgtacaag gttcagcaga
    13621 agcactggct gcatcattga agaagattga tgtggaaggt gtccgtgtaa aaatcgtcca
    13681 ttcagctgtc ggagcaatca atgaaagtga tgtaacctta gcagcagcca gcaacgctat
    13741 tatcatcggt ttcaacgttc gtcctacacc gcaggcaaaa gtgcaagctg atactgaaga
    13801 agtcgatatt cgtcttcacc gtatcatcta taaagccatc gaagaaatcg aaactgcgat
    13861 gaaagggatg ctagaccctg aatttgaaga aaaaatcacc ggtcaaatga tcgttcgtga
    13921 aaccttcaaa gtttctaaag ttggtacgat tgcaggtgcc tatgtaacag aaggatatat
    13981 tcgtagagat agcggtgtcc gtgtaatccg tgacggaatc gtaatctatg aaggccaact
    14041 tgcaagtctg aaacgattca aagatgacgt gaaagaagtc aaaatgggtt atgaatgtgg
    14101 tgcaatgatt gaaaaattca atgacatcaa agtagatgac gtaattgaag gatttatcat
    14161 ggaagaagtc aaaaatgact aaaaactgat aaaaggaggc caatcattat ggctaactac
    14221 cgtgaccgcc gagtcggtca agaaatctta aaagaagtca atgatatttt gcgcaaaaag
    14281 gtccgtgacc ctcgtgtgga gaacgtaacg atcacagatg tgaaagttac gggggactta
    14341 cagcaagcaa cgatctatta cagcatttta tctgacttag catcagataa gaaaacagca
    14401 caagatggat tgaataaagc atcaggattg attcgtcgag agttagggca caatctaagt
    14461 atttataaga cacctgaatt gttctttgaa cttgatgaat cagttgtcta tggcaaccat
    14521 atcgatgaat tgcttcgcga cttgaacaaa gactaatcaa aaactcccgc aaatcctgtg
    14581 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
    14641 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
    14701 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
    14761 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
    14821 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
    14881 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
    14941 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
    15001 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
    15061 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
    15121 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
    15181 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
    15241 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
    15301 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
    15361 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
    15421 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
    15481 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
    15541 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
    15601 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
    15661 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
    15721 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
    15781 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
    15841 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
    15901 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
    15961 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
    16021 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
    16081 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
    16141 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
    16201 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
    16261 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
    16321 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
    16381 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
    16441 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
    16501 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
    16561 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
    16621 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
    16681 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
    16741 gaagggtacg ttctgactgg acggcatgtt tttttgcaag ttacgaatca ttcttgcgag
    16801 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
    16861 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
    16921 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
    16981 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
    17041 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
    17101 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
    17161 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
    17221 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
    17281 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
    17341 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
    17401 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
    17461 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
    17521 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
    17581 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
    17641 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
    17701 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
    17761 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
    17821 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
    17881 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
    17941 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
    18001 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
    18061 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
    18121 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
    18181 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
    18241 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
    18301 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
    18361 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
    18421 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
    18481 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
    18541 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
    18601 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
    18661 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
    18721 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
    18781 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
    18841 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
    18901 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
    18961 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
    19021 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
    19081 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
    19141 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
    19201 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
    19261 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
    19321 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
    19381 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
    19441 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
    19501 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
    19561 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
    19621 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
    19681 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
    19741 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
    19801 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
    19861 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
    19921 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
    19981 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
    20041 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
    20101 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
    20161 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
    20221 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
    20281 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
    20341 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
    20401 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
    20461 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
    20521 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
    20581 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
    20641 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
    20701 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
    20761 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
    20821 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
    20881 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
    20941 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
    21001 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
    21061 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
    21121 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
    21181 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
    21241 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
    21301 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
    21361 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
    21421 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
    21481 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
    21541 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
    21601 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
    21661 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
    21721 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
    21781 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
    21841 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
    21901 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
    21961 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
    22021 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
    22081 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
    22141 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
    22201 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
    22261 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
    22321 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
    22381 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
    22441 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
    22501 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
    22561 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
    22621 ttctaacttt tatttctcta aa
//
LOCUS       DAYYHX010000027        22223 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000027 DAYYHX010000000
VERSION     DAYYHX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.027"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1936)
                     /locus_tag="ACHA6Y_000755"
     CDS             complement(44..1936)
                     /locus_tag="ACHA6Y_000755"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8986868.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(1933..3660)
                     /locus_tag="ACHA6Y_000756"
     CDS             complement(1933..3660)
                     /locus_tag="ACHA6Y_000756"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8986869.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3676..4326)
                     /locus_tag="ACHA6Y_000757"
     CDS             complement(3676..4326)
                     /locus_tag="ACHA6Y_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM8986870.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4530..4826
                     /locus_tag="ACHA6Y_000758"
     CDS             4530..4826
                     /locus_tag="ACHA6Y_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8986871.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5018..5638
                     /locus_tag="ACHA6Y_000759"
     CDS             5018..5638
                     /locus_tag="ACHA6Y_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8986872.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5730..6896)
                     /locus_tag="ACHA6Y_000760"
     CDS             complement(5730..6896)
                     /locus_tag="ACHA6Y_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8986873.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(6908..7261)
                     /locus_tag="ACHA6Y_000761"
     CDS             complement(6908..7261)
                     /locus_tag="ACHA6Y_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8986874.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7389..8411
                     /locus_tag="ACHA6Y_000762"
     CDS             7389..8411
                     /locus_tag="ACHA6Y_000762"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM8986875.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8430..8591
                     /locus_tag="ACHA6Y_000763"
     CDS             8430..8591
                     /locus_tag="ACHA6Y_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986876.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(8850..9284)
                     /locus_tag="ACHA6Y_000764"
     CDS             complement(8850..9284)
                     /locus_tag="ACHA6Y_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986877.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9394..11253)
                     /locus_tag="ACHA6Y_000765"
     CDS             complement(9394..11253)
                     /locus_tag="ACHA6Y_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8986878.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11254..11394
                     /locus_tag="ACHA6Y_000766"
     CDS             11254..11394
                     /locus_tag="ACHA6Y_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM8986879.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11371..12531)
                     /locus_tag="ACHA6Y_000767"
     CDS             complement(11371..12531)
                     /locus_tag="ACHA6Y_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8986880.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12838..14262)
                     /gene="araA"
                     /locus_tag="ACHA6Y_000768"
     CDS             complement(12838..14262)
                     /gene="araA"
                     /locus_tag="ACHA6Y_000768"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM8986881.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14278..14976)
                     /locus_tag="ACHA6Y_000769"
     CDS             complement(14278..14976)
                     /locus_tag="ACHA6Y_000769"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8986882.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(14933..16594)
                     /locus_tag="ACHA6Y_000770"
     CDS             complement(14933..16594)
                     /locus_tag="ACHA6Y_000770"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM8986883.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16812..18212
                     /locus_tag="ACHA6Y_000771"
     CDS             16812..18212
                     /locus_tag="ACHA6Y_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM8986884.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18464..19543)
                     /locus_tag="ACHA6Y_000772"
     CDS             complement(18464..19543)
                     /locus_tag="ACHA6Y_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8986885.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19629..20588)
                     /locus_tag="ACHA6Y_000773"
     CDS             complement(19629..20588)
                     /locus_tag="ACHA6Y_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8986886.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20600..22111)
                     /locus_tag="ACHA6Y_000774"
     CDS             complement(20600..22111)
                     /locus_tag="ACHA6Y_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM8986887.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLKSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
BASE COUNT         6304 a         4715 c         3931 g         7273 t
ORIGIN      
        1 cttttgtcac agcccctaaa aaaaataata aatttgtact attctatgaa gcttcttctt
       61 cagaaaactg actgttataa agatctgcat agaatccgtc tttttccatc aatgtatcgt
      121 ggtttcctgt ttctacgatc gaaccatgat tcatcacaat aatgttgtct gcatcgcgta
      181 ttgtagacaa gcggtgagca acaacgaaac ttgtccggtt ttccaatagg cggttcatcg
      241 ctttttgaat cagtatttct gttcgtgtat ctacactaga tgttgcttca tcaaggatca
      301 ggacatctgg atttgctaag aatgcccgcg caattgtgat tagctgacgc tgcccttgcg
      361 aaatattgct tgcatcctcg tttaatacag tgttatatcc ttctggcaat ttacgaacaa
      421 aatcgtctac gtgagcagct ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt
      481 tcccatattt gatattgtca tagatacttc ctgtaaacag ccaagtatct tgcagtacca
      541 ttgaaaactg tgcgcgcaat tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac
      601 gaatactacc tccagaaaca tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc
      661 ctgcaccagt cggacctaca atagcgacca tctcaccagg tttgacatcc aagttaaaat
      721 cagtcattag caagttatct ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt
      781 atggtgtatc tttttcttct ggaaggccag atttttcatt ggacatctct tcttcatcca
      841 acacttcaaa tacccgttcg gctgaagcaa ctgttgattg gatagtgttc atcaaacttg
      901 caagttgagt gattggttga gagaactgtg ttgtatattg caagaacgct tgaacatccc
      961 ctaatgtcat gttcccattt gcaactttta ctccgcctag tacagcgacg aacacatagc
     1021 ctagattttt aacgaaattc atcaacggca tgatgattgc cgaaataaat tgagctttcc
     1081 aaccagcggc atacagattc tgattttctt tttcaaatac ttcctggtct ttttcagtat
     1141 gattgaatgt tttaacaatg gtatgaccac catatgtttc ttctacttgg ttattcaata
     1201 aacctaaact tttttgctga tctgcgaaaa acacttgtga tttcggcgct acgatcatca
     1261 cgatgatcaa gcttaaaggg atcatcaata acgcgactaa cgtcaattgc cagctgattg
     1321 aaagcatcat cacgatgacc ccaatcaatg tgactgcact agttacaaat tgagtcaaat
     1381 tttgctgcag cgtgcttgcg atgttatcca tatcattgat tgctcgagac atgatatccc
     1441 catttgtatg tgtatcataa taggaaatcg gtacacgatt cattttggct tccaaatcat
     1501 gacgtaattc ataaactgtt cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt
     1561 tgaatacagc agaaatcaaa tacatcacga ttactgtcgc aatgatctgc gcgattttat
     1621 caaagtctat cggaaatttt gttacttgtt ggccttgttg catctgttga gcgccagcca
     1681 ttacaccttt gaatatttct gtggttgctt tccccaagac ctttggtgtt tggatttgga
     1741 agatcacggc tgctattgct agtacaaata cagcgatgat cgcaaccata cgtttagaca
     1801 tatagccaaa caatcggcgt accgttcccc agaaattttt aggcttttca ccttttacac
     1861 cgagatttct gccaggtcca ccatgtcctg gaccgcctgc tggtccatga gagggacgtg
     1921 tgtttgtttt actcatgcta agtcctcctc tcttagttgt gaatgaacga tttcttgata
     1981 agtttcgttt gttgctaata gttcttcatg tgttcctttg ccaaccagtt ttccttcatc
     2041 tagcacaagg atcatatctg catccattac tgtacttact cgttgagcaa ctaaaacggt
     2101 gatactttct ttcatattcg ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc
     2161 caacgctgag aaggaatcat caaacacata gatatctgct gttttgacta atgcccgtgc
     2221 aattgccaga cgctgtcttt gtccaccaga gaagtttcct cctccttgtt ccacatggct
     2281 atctaagcca tcttccatct ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt
     2341 ccaaatcatt tcatctgttg catccggctt accatactgc atattgtctc ggatcgttcc
     2401 agagaacaga actgcttttt gaggaacaaa gcccattaat tctcgcaaat tatattgagt
     2461 catgtcacta atgtccgttc catttatttg gatcgaacca gattcgatgt catataatcg
     2521 agggatcaaa ttgactaagg tcgttttacc tgaaccagtc ccccctatga ttgcgacgat
     2581 ctcgcccgac ttggcttgga aatcaatatc ttccaatgca agtttttctg ctccatgata
     2641 tcgataattg acatgattga aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt
     2701 aggattttca gggtcatgga taccgatttt ttcatccaac acttcattaa tacggtcagc
     2761 tgaagcttgt gcacgaggca ccatgataaa aatagcggaa agcatcataa agctgatcaa
     2821 gatctgcatc gcataagtca taaatgcaat cagattaccg acttccaaag tcatatctgc
     2881 tatgtagtgt ccaccaaacc aagtgattgc tacgtttgta ccgctcatga tcaatgtcat
     2941 cattggcatc atcaatgcga cgatcgtatt aactttgatt gccgtatttg tataatcttt
     3001 attcgctaaa tcaaaacgat tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct
     3061 gacccctgtc aatccttctc gaaataccag gtttaaacgg tcagttttct tttgcatact
     3121 tttgaataat ggaacagcaa agtacatgat tccgccgatc aaaataatca ttactgggat
     3181 gacaactaag aagatttttg tcaactgatg atctttttga taagcaagta tgctcgcgcc
     3241 aatcaacgtg attggtgcat tgatcatcat acgtaaaaac atttgtgtaa ccatttggat
     3301 ttgagtcaca tcattggtcg ttcttgtgat caaagaggcc gttccatatt tatcaaacgc
     3361 atcttttgtc agactctctg attttttata gacatccgta cgcaattgct tacccagttt
     3421 ctgtgattca cgagtagcaa aaaatgtatt tgcgatagca gctaagatac tgatcaaaga
     3481 aaagccgatc atcacaaaac ctgtatgcca aatataatcg acatctcctt gcgctacccc
     3541 tttatcaata atattggagg taagtgtcgg caaatataaa tctgccagta cttggatcac
     3601 catgaaaact gcagctgcaa ttgctgaagt caaggagatc cgttttacga tttttagcat
     3661 ctagttccct tctttctatt tatatactcc gtttttcagc caagaaagtt tcaaattgaa
     3721 ttcactgatt gcttgctcta ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg
     3781 cgcaattgtt gtaaatacag tattcatcac taattggatc agtaattcca attcatgatc
     3841 attttttact ttcagcttcg tccgatcgat caattcaagc aactcttttg catcttgttt
     3901 gtgttcttta tgtggatcgt gaggcgtttt ttggcgactc gtatgaggag cgacttttct
     3961 tgaggcacgg taatccatgt tcataaacaa gttacgataa aaagaagcat tctcgccttc
     4021 caaaatctca taaatcattt tagaaaaata ggattcgaat cctttgaata gatcaccatc
     4081 ggattctttc attagttgta acatatcttt agagctgtca cgtctcattg tctcaaagta
     4141 ataaaaataa agatcctctt tatcttcaaa atactgatag aaactgcctc tagggatatc
     4201 cgctaatttg acgatattag caatcgaagc ttcttttaag ggtactctag aaaactcgat
     4261 tctcgcagcc tcgattagtc ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt
     4321 cggcatatgg attctccttt ctttcaagat gacacattgt catatgacgc tatgtcatta
     4381 taacacctcc tacctgtttt gcaagacatt tgcatagttt ttttcattat catccgatac
     4441 attaaaaaag tgataagagg aatccttcca tttgaattaa tgagagttgc ccattaaaat
     4501 agccacatac taaacaaagg atgagataaa tggcccaatc aattagtgtc caagacttca
     4561 ataaattgcc tctcgatgct gacagcatcg ttttagatac aagaaatacc ttagattaca
     4621 caaaaaacca cttaagccac tcgctttcta tccccgctgc tatcttgcca agacaattaa
     4681 aaaatctaga tccaaagaaa ctttattata ttctaagcta tacaggcagg cgatcagaag
     4741 cattagccga tcaacttact gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg
     4801 aagctcttca gtcgagtgtt gcttgaatat tgcttgttag attcaaaaga ggctgtgaca
     4861 aaaaatcgtg tcacagcctc ttttataccc tgtctttttt tagttattta ttcttcaacc
     4921 ttatcaatat gacttgatag ccttttcttt ttcgagtaaa ctgattttgt tagtcatagt
     4981 tataacaatc atagcaataa aaggagaagg taagcatatg gcatctgttt tagtagtcaa
     5041 aggtcatccc ctaactgcag aagaatcgcg cacagttaag gcattgacgt cttttttaac
     5101 aagttataaa gaaaatcatc cagatgatga agtgactgtt ttggaacttt atcgcgatga
     5161 tattccggaa atcgatgaag aattattatc aggttgggaa gcattacgtg ctggtgcaga
     5221 attcacttcc ttatcagaaa gtcaacaaca aaaaattgct cgtttcaatg aattgactga
     5281 tcagttcctt gctgctgata aaatcgttat cgcaaacgcc ttatggaatc taaatatccc
     5341 aacaaaatta aaagcttggt tcgataccgt caatgtagct ggaaaaacgt tccgctatac
     5401 agaaaacggg ccagaaggat tagtgacagg taaaaaagca ctgcacatcc aatctaatgg
     5461 cggtgtatac aacggacaag actttgcctc tcaatatgta aaaggtattt taaactttgt
     5521 aggcatcgat caagtagatc agctattcat cgaaggaatc gattatgacc ctgaccgtgc
     5581 agatgagttg atgcaaaatg cattagataa agctgctgct ttaggtaaat cattttaaaa
     5641 attgagataa atgcatacaa aagagacaga ggaaaaaatc ttcaaagcag catttgtgcc
     5701 ttggatattt tcccatctgt ctctttttgt tattctttct gctttttcaa cggattttct
     5761 tcataaagag aagagattgt tgtgtaccat tcaaaaacat gagtaatcac tactttagct
     5821 gctgcataaa caggaatccc caagatcaca ccgatgattc cgaacaattt cccagaagtc
     5881 agcagaacta ataaaatcgt tactggatgg attgctaact gattccctag tactaatgga
     5941 gagatcaagc gtccttctat tgtctgctct actgcaaaaa cgatcagtac tttgatgatc
     6001 atgaatggac ctgctacaat ggctaaaaag atagccggaa tcatcgctaa aaatgatcct
     6061 aagtaaggga tcaggtttaa aaaacccgca ataatcccca acgtgacagc atactctaaa
     6121 ccaatgaccg caaaaccaat cataaacatg attgccacag caaaagccac agttagttgt
     6181 cctcttatat aagaagaaac ttgactattc atttcactca gcacattcaa cgtcggtttt
     6241 tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta attgtttacc gtctttcaat
     6301 agataaaata agataaacgg catcgtcagc agagccacta aaatagtcgc taccgcgccg
     6361 acaaaacttc caagactctg aactgtactc ttagatatat cttggatcat atcgcctgcc
     6421 gaattcatga acttatcacc tgctgtttcc aactgttcgc gaaattggtt gaacagcggg
     6481 tcccgcaaaa tctcggttaa tttattatca atcgtatcta catattgagg gaagttactg
     6541 atgaagctca ctgtttgttc ctgaatctta gggatgatca ctacgctccc ccatgcgatt
     6601 agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa gccttggtac ttttcgcttt
     6661 tcaaagtaat caactaccgg attcatcaag taatacaaga ttcctgctaa aatgataggc
     6721 agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca aatatgaaac ttttgtaaat
     6781 aaaaagacga tcagcaatat taacaataca acaagtaagg ctgtgactac ttgattattt
     6841 aaaaaccagc gccaaaacca cgataccggt ctgttcttct tattttttcc ttccataatt
     6901 cctcctgtta gttataaaaa atttggcttc ccaaaccaat agcaggcagt ctatgcggat
     6961 gaagatagat tccatttgca atttgtcctt cttctggaca agcatcaaaa atcaatgata
     7021 cgtgagctac tgtctttaaa ttcccattgg ccatcggacc gacataatcg atcgtatata
     7081 cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga aaaaggttcg tcttttgata
     7141 cttcttgaat caccgaatat tttcgtaagg cagctgttgc tgttttatcg aacaaaatca
     7201 gcatgggttc ttccttgttt attgcttctg tcccaatttc tgtaatcttt gcctgaatca
     7261 ttgcactctc tcctttaatt gctttcaaaa acagtataac acattctcat tctttagccg
     7321 tgtggaataa ttatgtgata gaataaaaac gaaaactatt tcattacaat taggaggaat
     7381 cagctcatat gatcgaaaag ataattttag gaacctatac gcgtcgcgaa agtaaaggaa
     7441 tctatacgat cgaccttgat acagaaaaag aagaattagc caacctttca ttagctgttg
     7501 aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg caatctttac acagttacta
     7561 gtgtggatgg attaggtggt gccggtgcgt acgataagga ttttcatttt ttgaatgcgg
     7621 taacagagtc tggcgctcct ttgtgttatg tagcggttga tgaagcacgt caattactat
     7681 acggggcgaa ctatcataaa ggtgaagtaa atgtctacca tatattagga gatggcgaac
     7741 tgaaagcagc agattcaata ttccatcaag aagcgacagg tcctcataaa aaccaagatc
     7801 atgcgcatgt ccactatacg gatctgactc ctgaccaacg attagtcgta tgtgatcttg
     7861 gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg caaattgaat cttgtaacta
     7921 ccttcactgc agagccagga acaggccctc gccatcttgt ctttcatcca aatggacaat
     7981 tcgcttattt atttggtgaa ttagacagca cagtccgcgt gctttcttat aataaagaaa
     8041 caggtgcttt tgaagaatta cagaaaatat cgaccttgcc agaagaattc gacggtgaaa
     8101 atggaggggc agctatccgc atttcaaatg atggcaagtt cctttatgct tctaaccgag
     8161 gacataactc gattgctgta ttcgcagttt ttgaagatgg ttcccacatt caaaacaagc
     8221 aaatcatctc aacagaaggg gattttcccc gcgatttcgc actagatcca agcaataatt
     8281 ttgtcgtgtg tgccaatcag aacaccgata atctgacatt atatcgccgt catcctgaaa
     8341 caggattact ggaattactg aaaaaagatg tctatgtacc agaatgtgtc tgtgtttatt
     8401 ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca agacgtatta gacagactac
     8461 gggaagaact aaatattcct ttctttgatg ggaaaatcga agataaagag tatagcgaag
     8521 aggaatatca gaaactcaaa gccgatttac agaactactt tgaacaatat gtacagaatg
     8581 tagaaaacta atcagctttc catactatct gaaaagaaca tcaggacaag acttttccgt
     8641 ctatcctgat gttcttttct acaaataaaa aaccgccgag atggttagac tcgacggaaa
     8701 aggagtattt actcgatttt gagtaagtac aaggttgttg ttggttatag gtatgtcatt
     8761 atcataaagg attttgatca ctctatcata aaggtaatat tacatttata ttagataact
     8821 ctagttttta ttgttatcaa agatttcttt tattttttca acaacaaatt agtcattttt
     8881 tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt ttgcttcaga tgtattgaat
     8941 agatcttcat ctacccctga ttcaagatcg atactttgga tcatttcttc acagcctgct
     9001 acatatgaaa gataagcttt ttcaaaattt ttatgaatgc ccattgcttt tgctggtgga
     9061 cgcaaagtac tgactttcat aagcattgcc cgatattttt ttgttccttc ttcaaataac
     9121 aggatgatct cttcaagagt cgatttgtcc agttcacttg tttttccttc atcaattgcg
     9181 tttctgatca cttcatattt cggatgcatt ccttctccga ttgcttctgt ttccttgacg
     9241 atttgatcga tggactgcac ataatagcca atattcggta acatgttcgt tttcctactt
     9301 tctccattgt tgttattatt tattctactt gtttttatcc tcaaatgaca agtacttgaa
     9361 aaaatttaat ggtttctata cagctcgcca ttcttattcg tgctcaaaga aaccatctgc
     9421 taaactatat tgtttccctg cgacaatgtc atattggatc aattgatatt cgtcgtaaat
     9481 ctcttgttgc gagcgattga attgcgcttc tttataccac tgtccgtttt gatagtacag
     9541 ttctcgttca aacgccggaa gttcctgttc taatgataaa agcagctgat aaaagccatt
     9601 tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa taaaacggac tggtgattgc
     9661 atcgtgagtc gtttgctttt ctaattctcc tttactgtcg aacataagaa attgtgtttc
     9721 atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct gaatagatcc ctggaagatg
     9781 atccccccaa aaaacaacta aagtccttct agaaagattc ttcaattctt ccgtgaactg
     9841 cttcagtgcc gtgctgctat aggaaatatc ttgcagataa ttctctaagt caagtgtccc
     9901 acttccatct gaagtttttg caggatagtc tagattatca tactttccat cgtagggcat
     9961 atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt ttttcttcct tgagaagtgt
    10021 cattacttct ttgtaagctg actcatcaga aatatatgga ttattctcta tcgtatccgt
    10081 ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct aatgtctgat aaacatcctc
    10141 tcttttatac atcgaagtat tgtatggatg gatcgctgtc gtctggtatg attgtgcgtt
    10201 taacgctgaa acgatagaag gaagctgatc catttttggt acaagcatcg tgtaaggggt
    10261 ggtgagttgc gcattaaata acgccataga aaaaccagtc aatgcttcaa attcgatatt
    10321 tgctgttcca ccgccatagt tttgagaaag catattgcca ctatatgttt gatcagctac
    10381 ttcataataa tctgccaatg gctcaccaga tacttctacg ccgtttaatc gactaggatc
    10441 tgagaaactt tcactcatca cgaacacgat gttcggcgac tcttcctcta cagttttatt
    10501 tttttcatcg gctagcttct ggtatttttc agtgatttct ttgatctttt ctttcgaata
    10561 tccttcaggt tcatccattg gttctacctt cagattgtac aaaaacccac cgataaaacc
    10621 agtgttatag tagttcattt tctggctgta aggtatccat agtgccgttt tattatatgc
    10681 ttttcgcaac aaattatcgg gattgttgaa attactgata taccctagca gtccaattgc
    10741 cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt ccttttttca ggcttcgaaa
    10801 catgtaccag caaaacaatc ctatcacggt tcctgcggca gctaagatca ctgtaaacag
    10861 catagtgccg gtcatatctt tcagcaatcc gatttcagtg atcatcttca aatcgtctgg
    10921 ataaatcggt tcttgccgat aactcatttt catataattt gcaaaaccta atacaccgat
    10981 ggctacgaca tacgtaaggg aacctaaagg gacagatcct gctagtgcaa tcagaaaaat
    11041 aagaataatc agcaagacga gacaggctaa gaagaatttt tccgtatgcc aagaaaaagc
    11101 aaatttcaac gcaaggtcta cggatagatc attttgacac cactgcaaaa ataaattact
    11161 aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt ccttttaaaa tctttttctg
    11221 tttggtcgaa agtctgctta tatatctttt caaatgcttg ctccttcatt tctatcaata
    11281 aaagaatgtt gcttctctta ttttagttgt acgaaattcg aaagtaaaga acggaaagaa
    11341 gaattaatga aatccaaata ttctctttct ttatggcctt tctggtttct ttaataagcg
    11401 gacctttcgt actccagaaa ttttttctag ctgttgtttc acttgattga gctgttcaaa
    11461 atcagtactg tctatgtcaa tcaatatgta gacatgctca ttcttatggt tacgcgatat
    11521 atctgcgatc gctagctcgt tctcattcag cagagaaaat atctctgcta atatattggt
    11581 tttcttttga taaaatatag tgaatcgaaa cggcgcttgg aaaaacattc gtgcggatgg
    11641 ataattgacc gcttctctaa ccgtaccgaa caaaagataa tcttttaagg cttcaactgc
    11701 caaacgtccg ctgtctgcca aagcttcctg agtcgatccc cctagatgag gcaacataag
    11761 tatacggtca ttttctaaga actcttcttc tggaaaatca gtcaagtatt tagccagatg
    11821 ttgtttttcc aatgcttgca taacagcata tttatcaaca atttcacttc ttcctacatt
    11881 gataagtaca gcatcttttt tcattttttc tatattcgcc tgatcgatca atccttttgt
    11941 atcttctgtc aacggcagca tgactacgat gtaatccgag gtagaaagaa ctgtttctaa
    12001 atctgcttgc cgaacataat ccaactgggc atcgcggata gaataaccca agacatccat
    12061 ccctaagcta tagcaactta gcgctacttt tgtgcctatc gctccaagac caagaagacc
    12121 gatggttttg ccttgcaatt ctcgtcccac ataagcactt cttttgtttt ctgcctgttc
    12181 taaaatatta ggacctgtta gtgtttgtac catacgactt gcttcgatta tcggacggga
    12241 agataagagc aaacagcaca aaacaagctc ttttactgca ttagcattta cacctggtgt
    12301 attcatgacg atcgtcccgt tttcagaagc tttttcaacg ttgatcgtgt tcaccccaac
    12361 accagctctt gaaattgcta gcaaatctgg tgtaagccat tcatctttca ctttcgtact
    12421 acgaataact attccttcgg ctggcaaatc atctattatt ttgaaatatt ccccttgtaa
    12481 cttttctatc tcttgttctg gaatagcatc aatcaaatga acgtgataca taaaaccccc
    12541 tccttactaa caagtatctc gaaatttctg caattagaca actgtttacc atttgacttc
    12601 ctagaagcaa aaagagcagt tagagcagag taggctgagt gataaaaata aggctatgac
    12661 aagttactgc cataagcctt attttcgaga gcaaaatatt gagggtgaga tatcttgata
    12721 aatggtgttc cgttagctga cattgataat aagactaaat ttcaaaaaag ttgaaaagca
    12781 aatttcgtca atctcttctt atttcttaaa actaagtggc tttttcacaa cctcttttta
    12841 tctaataacc actgtttcca actcaactaa ttttgcccac gtttcaattt gatctgttgt
    12901 tagatttagc gagacaacgg tatgatgacc gccacctgcc tgaatccatg aatggacacc
    12961 ttcatggaaa ttcggtttag ttttccataa cacacgtgca acaggtaaat taggtgcttc
    13021 agtcgttggt tcaaaggctt ctacttcatt gatcagcagt ttataatgcg taccgaaatc
    13081 tgccatcgat acgactaccc cttctcctgc tttcccatca aatacaagac gcgccggatc
    13141 ttctctatct cccattgata aaggagaaac aacgattctt ggtttcgtcg cagcaagcgt
    13201 cggatctact tccatcatat gggattccaa gattgcttct tggccactag ctagctcgta
    13261 agtataatct tccatgaatc ctgtatcttt ccctcgagcc atgattttca gcaaccgatc
    13321 aatcgctgca gttttccagt cgccttcccc agcaaaaccg tatccttctg ccatcaaacg
    13381 ttgtacagct agtccaggta gttgtttcat tccatacagg tcttcaaaat tagtcgtaaa
    13441 agcgttatag cctccttctt ctaagaaacg gcgaataccg atttcctgct gagcttgtac
    13501 ttttacatgt tcctcccatg tcttttcttc ataatcacca tagtcgaagg tatacaattc
    13561 cttgtactct tcaaataatt gatcgacttc ttcgtctgat acgcgatcga ttacttgtac
    13621 aagatctccg ataccaaaat aatcaacagt ccaaccaaat tgaatctgtg cttccacttt
    13681 gtctccttct gtgacaccca cgtttctcat gttgtcacca aaacgagcca cttttattcc
    13741 aaaactttca ttataggcta ctgcaacatc catccaatca gcaatctctt tttggatctc
    13801 aggtcgtttc cagtagccaa cgacgatttt attttgttta tttaatcgag cattgatgaa
    13861 cccatattcg cggtcaccat gagcggattg atttaaattc atgaaatcca tatcgatcgt
    13921 tttccaagga atgctttcgt tatattgcgt agctaaatga agcaatggtt tttgtagaag
    13981 ctttgtcccg cggatccaca tttttgctgg tgagaaagta tgcatccaag tgatcacacc
    14041 tgctacttct tctcgataat tgacctcttt catgattttt gtgatcgtat cagcagtaac
    14101 tgctaattct tgaaggcgga ttggataagg caattggcca ttttcattca gttcatcgac
    14161 catttcttgt gcatgtttct ttacttctcg cagtgcttct tccccatata aatgctgtga
    14221 tcctacaaca aaccaaaatt ctttttctcc aatattcaac ataattagcc actcctttta
    14281 tttgttttgt ccgtaatacg cattttttcc gtgttttcgt aaataatgtt tatctaaaat
    14341 ccgttgaggc agctcttctg aaaatgaatt gagctgtctg gtaaataaat tcatcttgca
    14401 tacttcatct agtacgactg cgttcattac cgcactttgc gcatctttcc cccaagtaaa
    14461 tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag ggatcaattt tgcgttcttt
    14521 gaatgtttct atgataactt ttcctgtttc atattcgtac ccgctgtcga tttcctgttg
    14581 tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta tctgcatgcg tcgtccccat
    14641 cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc catgtcgagt gagtatggac
    14701 gattcctccg atttgaggaa atgaacgata taaaacagca tgagtaggcg tatccgaaga
    14761 gggattgagt ttaccttcaa tgatatgatt gtccaagtcc acaacgacca tgtcgtctgc
    14821 tgtcatctct tcataactga ctccgctagg tttgataacg aaatatcgtg tttcaggatc
    14881 aaaagcgctc acatttcccc atgtatattt gacgagtccg tgtttaggca agtcaagatt
    14941 tgcttggtat acttcttctt tcagttgttc cagcattttc tatcactcct ttttcttgca
    15001 attgatgaat cgctgttttt tcaatgtcga tcccctcacg gtaacgtctc ataaatatct
    15061 gatagcctgc tatttcttct gctgaaggtg tgatggtgat tccttggtct tgtccaaaca
    15121 cgtcttctgc caagaagttt tctaaagact tcccatgtcg atccatcata taggctgcta
    15181 ataacgcgat tccccacgct ccaccttctc cggctgtgtc catcactgta actggtgcct
    15241 ccatagcaga tgctagaatc cgctgtccaa cttctggtgt tttaaaaatc cccccatgcc
    15301 ccactaaacg atcaatttgg acattctcag atttcaatat atccattccg attctcattg
    15361 caccaaaagc actggctaaa tggatccgca tgaaattcgc taaatcaaaa cggctacctg
    15421 gtgtacgtac aaataatggc cttccttcat ttactcctgt aatgttttca ccggaaaaat
    15481 aaccataaga aagtaatccc ccgcaatccg tatcgccttc tagcgctttg ttgaataata
    15541 ccgtgaataa tttatctctg cttatctcca tacccaaaga attggtaaat tcttcaaaaa
    15601 tactgaccca agcattgata tccgaagagc agttattcgt atgaaccatg cctactagtt
    15661 ctcctgaagg tgtagtgacc atgtcgattt ccggatggac attttctagt tctttttcta
    15721 atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt ccctgtccct ttggctacac
    15781 tgttcgtagc gaccataccg gttcctgcat ccccttctgg cggacaaacc gggattcctg
    15841 gtctcaaatt tcccgtaggg tcgatcagat aagcccctga ttcgcttaac ttgccagctt
    15901 cttctcctgc tactagaact ttaggcaaaa tgtgatccaa gtcaatcgct acgtgcgctt
    15961 tttcgattaa ctgaccaaaa atatttttca tttcttgatg gtaggttttc gttgaaggat
    16021 cgataggaaa catgcctgat gcatctccta cgcctagtac tttctcacca gtaagctgcc
    16081 aatggacata acctgcaagc gttgtcaaaa aattaatttc tttcacatgt tcttctttat
    16141 tcaaaataga ttgatacaaa tgagcgatac tccatctctg cggaatattg tattgaaatt
    16201 tttccgttaa tattcgttct gcttcttcag tgattgtatt tcgccaagta cgaaatggta
    16261 ctagtaactg ttccttgcta tcaaatgcta gataaccgtg catcattgca ctgaacccaa
    16321 tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa tacttctgct gccatttttc
    16381 ggtaactgac gcgcagtcct ttccaaatat cttctaatga ataggtccag ataccatttt
    16441 ctagtttgtt ttcccactca taactaccag cggcaatagg agtaaaagat gaatcaatca
    16501 atacagcttt gatcctagtt gatccaaact caatccctaa agatgtacga ccatctatga
    16561 catctaacat actttgcgca tttacttcct tcataatgat ttaactccct ccttctcgtc
    16621 aaacctgatc aaaccgtctt tttagtagcg cttacaatga ttattgtata cttttgtacg
    16681 tacatgtcaa gcagttattt acaagaatac aataaaaaac acttgttttg tacgtacata
    16741 agttataaaa tgaaattgct tacaaaatct gatcaaccgc cttctcgaaa ttctagaagg
    16801 agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc gtttatttat ttttttggcg
    16861 catttggcgg cattctattt ggatatgata ttggtgttat gaccggcgcc ttaccttttt
    16921 tacagcatga ttggggtttg gcaggtaaag cttctttgat aggatggatc acgtcttcgg
    16981 tcatgttagg tgcgatcctt ggcggctctt tatctggtct tctttctgat aagcttggac
    17041 gacgaaaaat gattcttctt tctgcactta tttttatggc tggttctgtc ctttctgcat
    17101 ctgcccctca caatggttct tattttctga ttgcagcaag gattcttcta ggtttagctg
    17161 ttggcgctgc atctgcccta gttcctgctt atatgtctga aatggctcct gctagactaa
    17221 ggggaagatt gtcagggatc aatcaagtga tgattgcttc tggcatgctc ctttcctatg
    17281 ttgcagatta tttgctaaaa gggttgcctg aaacgatggc ttggcgcgtg atgttagggc
    17341 tcgctgctgt ccccgcgctg attttatttt tcggtgtttt agctctacca gagtcaccgc
    17401 gttttcttat gcaatctggc cgcttggaag aggcaaaaag agtattgaac tatattcgga
    17461 caccaaaaga agctgaacag gaattcgaac aaatccagct gaatgtcaaa caagaaaaga
    17521 caaccggaac ttcttggcat actttgtttt tagaaaaata tcgttcctta gtcattgccg
    17581 gtattggtgt agcagtattc cagcaatttc aaggagctaa tgctattttt tactatattc
    17641 cgctgatcgt agaaaaagcg actgggcatg cagcgagcga tgcattgatg tggccgatca
    17701 tccaaggaat cattttagtt gctggttctt tgctcttttt ggttattgca gacaagttca
    17761 atcggcgaac tttattgaaa ataggcggta gcgtgatggg actttctttc atcctaccag
    17821 ctgttttagg aactgtactg gatgctcata caaattcgtt gttgattctc ttgtttttat
    17881 gtatttatgt cgcattttat tcttgcacat gggctccact gacatgggta atcgtaggcg
    17941 aaatattccc actagctgtc cgcgggcgag catctggcct agcctcttct tttaattgga
    18001 ttggttcttt cctagttggc ttgctttttc ctgtgatgac tgcttctatg tctcaagaaa
    18061 tcgttttcgg catttttggg atcatctgct ttttaggggt tttatttatt caagagatcg
    18121 taccagaaac tcgtgggaaa tctttagagg aaatcgaaca aagtgcatct aaaaaaactt
    18181 atcctaaacg tatttctatc catcattctt aaagaggttg tgacagaagt ggtcagcttc
    18241 aagaaataag aaggaaccgc ttagtttcca gtcacaagga acaatttcaa aaaacagctt
    18301 ctcatgtttg atgaatattg tgacttgtga ccagttggta ggagaacacc gtttagtgga
    18361 gaggtggtga aagaaacgtt cagccttgag cgatgacaaa agctctcaca aacattgcga
    18421 cgagtaaccg taggagcaat aagaggcggt aaaaacccgt ctcttatttt tcgttttctt
    18481 tcactgtttc ccgttcaact agtacaggtt catagagaat atttggctgt gcctgtcctt
    18541 tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc caacttttct tttggatgcg
    18601 tcaatgttgt caatttttta ttccctaaat ggcttaaaga agaatcgtct gttcctacga
    18661 tcgataggtc atctggaata tgatagccat tttctgtcaa cttatctagc attaaactag
    18721 ctatttgatc gttatagcaa atgatgccat ctaaagcttg ttcttctttc aaccgttgta
    18781 ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa agtaatgata tcttccgtca
    18841 aaaaacgcac attatactgt tcacacgcgc tgataaaacc tttcatcctg tattttcctt
    18901 gaagatcgtc gatttttgtg atgaacaaca gacgctgatg tccttttttt atcaaatatt
    18961 ctgctgccat aaaaccgact ttcacatcat ctaaacaaat ataagaggca tttaactctt
    19021 cataagcagc attgatcatc accatcggaa tgccttgttc tctcaaagca acataaagtg
    19081 ctaagtttgg gttatactga ttacttttcg taggctcaac gatcaaacca tctaccccat
    19141 aagcaatcat tttcttcaaa cactctttct ccaattcgtg atcgttattc gtactggcta
    19201 aaagcaatga atacccagat tctcgtaatt cattttcgat tcctcggata atcgacggaa
    19261 aaatataatc agagagatat gtggtgatga caccgatcgt ttttgttctg ttcaatggtg
    19321 ttttttcttt cttatagctg tcatctacat atgtgcccga ccctttttct ttacgcagat
    19381 agccctcatt taccaacaca gcgattgctt gccgaatcgt atgtctgctg acgtcatata
    19441 tttcttgcag cttgaattct gagggaatgg ttgtatttac ttcgtattcc tcacgaagga
    19501 tcttttgttt tatctcttct gcaatatctt gatattttgc catttgcctt gccccttttt
    19561 atttgtacgt acatatatca taacgagtct gcaaggttta ttcaagcaaa aatcaaggtt
    19621 tcttgtgctc ataaggcata cggaacaggt tcaccaaatt ctggaaatcc ttgctcattc
    19681 cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt ctaacggatc ccctttttgg
    19741 ttcttctctg gtcgagcatg atagatcaaa atatcaaagc acccgtcttc tgaaaccgta
    19801 aacgagttgt gaccaggacc aaattgctta tttttttcag aagaaacgaa tactggctcc
    19861 gataatttat gccatgattc ttttaataaa agattcttgt gctcatcagc ccacagcagg
    19921 cccatggcat agttttcatc tgttgcacta ccagaatacg taatgaatat tttcccattg
    19981 cgagtaagga ctgctggacc ttcatttact gaaaaacccc gtttttccca ttcatactca
    20041 ggtatagaaa ggaggatctg cttccccttt aacgtccaag gattatccat ttcggataga
    20101 taaagattag aattcccagt aatttcagga tcttgttggg cccacacata atatagcttt
    20161 ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt cccttttcgt ttttatttgt
    20221 cccttttcta tccaatctgt attcattgga tctatatgct gattttcaag tacgaacatg
    20281 cggtgatgat gagattcatc acgtatagtt ttatgattgc ttgcagcaaa gtaaatatac
    20341 cattttccat tgatataatg gacttcaggt gcccagatca attcactcat cggtccttct
    20401 tcatgagcct gccatacaat caggcttgca gcatgttcta attcattcag tgactttgcc
    20461 cttcttaatt caatcacttg gtagcctgga accgatcctg taaaataata ataaccatct
    20521 gtatgtttat atacccaagg atctgcccgt tgccgaacga taggattatt ataaagtgtc
    20581 ttcatcataa caaatttcct cacattttct ttattctaat tacattccaa gagtattttt
    20641 ctatccctac atttaattcg tcatgttttt ctattgaaaa atagtttatt tcaggtttta
    20701 ttttttctgg ttcttcacgt gtatttttgt ccaacagatc gctactggtt aatgtgtagt
    20761 gctctccttg atctagcaaa ttgaaatcat gttcaatcgt tatcttttgt tgttcatttg
    20821 tgcgattgat caagaaaata gtcagctgat tgccgttatc tacgatcaca ctgtccacat
    20881 aaggaacatt ttttatcttt tcacaatcat aaacagggca aatgatttgt tgtttcaata
    20941 cttgaccttt agcatatttc gataagtgtt gcatcacata ataagttgga ttcctccatg
    21001 cttgtccttt agcatctgtc agtattaaag gtattgtgtt tactaacaat gattgacaac
    21061 taatctttac gcgatctgcg tgtcgtaaaa tagccagccc taatgaacca aataataatg
    21121 tatcttccat agtaaagtaa caccaatcga tgggactacc gacttcaaaa tccctatgtc
    21181 ttttttccgg ttgggcatgt acattccatt catcaaacgc tagatacatt gttttctcac
    21241 ttttcaaata tccttttaca taatcacaag tagcaacgat ttcttcaatt tgacgatcga
    21301 atcgataagc ttttgcaagg tatgtcgctg tatcatcacg ctcattttta ggaggtttag
    21361 tcaagtcttc ttcatcatac ttatcaatat agttatgcaa agctaaataa tctacattct
    21421 cataagcttc ttctaatgcg atacgatccc attcagggta cgatggtaaa cgtggcgtag
    21481 agcttccaac taagattgtt tcaattgaat catccactaa cttcatgact tttgaggctt
    21541 catttgctaa cctaccatat tcataagcac tcttagcccc cacttgccat ggaccgtcta
    21601 gctcattccc taaacaccat aatttaacgc caaaaggctt tttttgccca ttttttattc
    21661 ttaaatcact ccagaaggtt ccttttggaa aattacaata ttctactaaa ttttttgctt
    21721 catcaattcc ttttgttcct aaattcaccg tgaatatcgg tttcgcttgt acatgactga
    21781 tccatttaaa aaattcgtgg agtccaaaag aatttggttc tatacctcgc catgctaaat
    21841 caattttttt tggtcgttgt tctttcctac ctacagtatc cacccaatca taaccagatg
    21901 taaagtttcc tccaggatag cgaatgacac ctaattctaa ttcctttaca taagagagga
    21961 catcttgtct aaacccgtca ccatcagctg tttcatggcc cggttcataa atccctgtat
    22021 agacgacact tcccatatgc tcaacaaatg agccaaacag ccgagaactg atctctccta
    22081 ccgtaaaatc ctctctgatt tttactttca cttcttcgtc caacagcttt ccctaccttt
    22141 catttcctat agtcaagata atagatacaa gtttccatac gccaccagca aagataaaaa
    22201 taaaaggagg gaaaagaaat atg
//
LOCUS       DAYYHX010000028        22011 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000028 DAYYHX010000000
VERSION     DAYYHX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.028"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACHA6Y_000775"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACHA6Y_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM8986888.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACHA6Y_000776"
     CDS             complement(2020..2922)
                     /locus_tag="ACHA6Y_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8986889.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACHA6Y_000777"
     CDS             complement(2919..3611)
                     /locus_tag="ACHA6Y_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM8986890.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACHA6Y_000778"
     CDS             complement(3654..4295)
                     /locus_tag="ACHA6Y_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM8986891.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACHA6Y_000779"
     CDS             complement(4333..4647)
                     /locus_tag="ACHA6Y_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986892.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHA6Y_000780"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHA6Y_000780"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM8986893.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHA6Y_000781"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHA6Y_000781"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM8986894.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACHA6Y_000782"
     CDS             complement(5984..6916)
                     /locus_tag="ACHA6Y_000782"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM8986895.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACHA6Y_000783"
     CDS             complement(6913..7689)
                     /locus_tag="ACHA6Y_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM8986896.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHA6Y_000784"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHA6Y_000784"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM8986897.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACHA6Y_000785"
     CDS             complement(10933..12009)
                     /locus_tag="ACHA6Y_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM8986898.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACHA6Y_000786"
     CDS             complement(12014..13294)
                     /locus_tag="ACHA6Y_000786"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM8986899.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACHA6Y_000787"
     CDS             complement(13313..14239)
                     /locus_tag="ACHA6Y_000787"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM8986900.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACHA6Y_000788"
     CDS             complement(14252..15535)
                     /locus_tag="ACHA6Y_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM8986901.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHA6Y_000789"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHA6Y_000789"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM8986902.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACHA6Y_000790"
     CDS             complement(16323..17234)
                     /locus_tag="ACHA6Y_000790"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8986903.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHA6Y_000791"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHA6Y_000791"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM8986904.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACHA6Y_000792"
     CDS             complement(17700..18398)
                     /locus_tag="ACHA6Y_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM8986905.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACHA6Y_000793"
     CDS             complement(18385..18771)
                     /locus_tag="ACHA6Y_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986906.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACHA6Y_000794"
     CDS             complement(18918..20588)
                     /locus_tag="ACHA6Y_000794"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM8986907.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACHA6Y_000795"
     CDS             20771..21004
                     /locus_tag="ACHA6Y_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8986908.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACHA6Y_000796"
     CDS             complement(21103..21534)
                     /locus_tag="ACHA6Y_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM8986909.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..>22011
                     /locus_tag="ACHA6Y_000797"
     CDS             21613..>22011
                     /locus_tag="ACHA6Y_000797"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM8986910.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKE"
BASE COUNT         6222 a         4922 c         3888 g         6979 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga a
//
LOCUS       DAYYHX010000029        21821 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000029 DAYYHX010000000
VERSION     DAYYHX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.029"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..223)
                     /locus_tag="ACHA6Y_000798"
     CDS             complement(26..223)
                     /locus_tag="ACHA6Y_000798"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8986911.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(369..1247)
                     /locus_tag="ACHA6Y_000799"
     CDS             complement(369..1247)
                     /locus_tag="ACHA6Y_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8986912.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1286..2404)
                     /locus_tag="ACHA6Y_000800"
     CDS             complement(1286..2404)
                     /locus_tag="ACHA6Y_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8986913.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2401..4500)
                     /locus_tag="ACHA6Y_000801"
     CDS             complement(2401..4500)
                     /locus_tag="ACHA6Y_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM8986914.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4551..5372)
                     /locus_tag="ACHA6Y_000802"
     CDS             complement(4551..5372)
                     /locus_tag="ACHA6Y_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8986915.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5369..6178)
                     /locus_tag="ACHA6Y_000803"
     CDS             complement(5369..6178)
                     /locus_tag="ACHA6Y_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8986916.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6199..6693)
                     /locus_tag="ACHA6Y_000804"
     CDS             complement(6199..6693)
                     /locus_tag="ACHA6Y_000804"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8986917.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6705..7205)
                     /locus_tag="ACHA6Y_000805"
     CDS             complement(6705..7205)
                     /locus_tag="ACHA6Y_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8986918.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7270..9966)
                     /locus_tag="ACHA6Y_000806"
     CDS             complement(7270..9966)
                     /locus_tag="ACHA6Y_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8986919.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10394..11467)
                     /locus_tag="ACHA6Y_000807"
     CDS             complement(10394..11467)
                     /locus_tag="ACHA6Y_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8986920.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11464..12291)
                     /locus_tag="ACHA6Y_000808"
     CDS             complement(11464..12291)
                     /locus_tag="ACHA6Y_000808"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8986921.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12292..13098)
                     /locus_tag="ACHA6Y_000809"
     CDS             complement(12292..13098)
                     /locus_tag="ACHA6Y_000809"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8986922.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13110..14195)
                     /locus_tag="ACHA6Y_000810"
     CDS             complement(13110..14195)
                     /locus_tag="ACHA6Y_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8986923.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14212..14754)
                     /locus_tag="ACHA6Y_000811"
     CDS             complement(14212..14754)
                     /locus_tag="ACHA6Y_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8986924.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            15012..16001
                     /locus_tag="ACHA6Y_000812"
     CDS             15012..16001
                     /locus_tag="ACHA6Y_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8986925.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            15998..16735
                     /locus_tag="ACHA6Y_000813"
     CDS             15998..16735
                     /locus_tag="ACHA6Y_000813"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM8986926.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            16732..17283
                     /gene="coaC"
                     /locus_tag="ACHA6Y_000814"
     CDS             16732..17283
                     /gene="coaC"
                     /locus_tag="ACHA6Y_000814"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM8986927.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17299..17931
                     /locus_tag="ACHA6Y_000815"
     CDS             17299..17931
                     /locus_tag="ACHA6Y_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8986928.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            18016..18663
                     /locus_tag="ACHA6Y_000816"
     CDS             18016..18663
                     /locus_tag="ACHA6Y_000816"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8986929.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18668..19651
                     /locus_tag="ACHA6Y_000817"
     CDS             18668..19651
                     /locus_tag="ACHA6Y_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM8986930.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(20054..21568)
                     /locus_tag="ACHA6Y_000818"
     CDS             complement(20054..21568)
                     /locus_tag="ACHA6Y_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM8986931.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         6573 a         4731 c         3781 g         6736 t
ORIGIN      
        1 tattttatac ggctgaaaaa atctatcacc aaaaaaattc aaacattaag ccaaggatta
       61 tgaagtcaac atttcgaaaa atgaacatac attggtaagc aactataact attaccacag
      121 acataaagta acacaatata ttactattta agtgtacgcc accagtgaat atcctcaagg
      181 tatacagcgc acaacaaaaa gaaaaaagga aacgcagaag catttccttt cagtgattta
      241 atgaaaacaa cacgatttga cttagagcct ttttatttga attcattttc gctttatcca
      301 acttcatttg atcatttgat tactcggtta aatacttttg acatgagtat caatgaaaca
      361 ttcattaatt atggttgctt atttcttacc gcttttctgg ccaatgctaa tccgccgatt
      421 attccggcat tatcacctaa ttcgggcgta ataatgtagc tatcaattgg tggtatttca
      481 acatatccat ttataagctc aacaaattta tcctgaactt ttttcttcat gtgttcttgt
      541 ttcattacac caccgccaaa aataattcta tctggcgaaa acatcagtgt tgtattgtat
      601 gcacattggg caaggtaaaa ggcttcaatc tgccaaaacg aatcgtcagc caataaattc
      661 tggcctttta cacccaaccg tttttcaatc gctggtcctg ctgccatccc ctctaaacaa
      721 tcttggtgga agggacagtt tcctgaaaaa ttgtcctttg gatgtcgctt gatcaacata
      781 tgccccattt ccggatgact gaacccttcc acgaaagtcc cattgaacaa ggcaccacct
      841 ccgatacctg ttcccacagt gaagtatgca acacctgcta accctttgcc agaaccagca
      901 acatattcac catagcaagc cgcattgaca tctgttgtcc attcaatcgg aatattgaaa
      961 tattgcttta gcgttcctac aaaattgaaa ttttgccaag caagttttgg tgttgatgtg
     1021 atatgaccga aactcgttga tttgcattga atatcaattg gaccaaagca gccgaccccg
     1081 atgcctgcca attgttcctt aaaaggttta aaaaaatcaa tgacctgctt cattgtttca
     1141 actggagttg ttgtgggaaa actgactctt tccaaaatcg ttaattcctc agtaccgact
     1201 gcacagacaa atttagtccc tccagcttca attgccccat atatcatcat aggttcctcc
     1261 cttcgaatat gtgaaaaaca cttcattaac gcggcaagcg ttctcgatca gtgaaatgac
     1321 gtttagtatt tcctgaagct tctgttgaat accagtaagc gacactagca acatcgtctt
     1381 gccgctcaaa taagcgaaca tcatcgttgc cgatttgttg taaagtgacc gataaatctt
     1441 cggtaaaaca gatggggtct tgaagatgcc aacgataaag accatgtcgt ggcattgcgt
     1501 cgtcaccaaa ggcatgaacg gcattcaatt ttcccgtttc aaaacgatct cttgtggcat
     1561 ctctatttga ttggtaagga taccctaaaa aggctgttga atagtttttc gcatgtggac
     1621 gtccgaactc atcgtactca tgaaaggccc acgccccgcc aaaataatct tctgaacctg
     1681 ttgaggttac ggtcggatac tcactatccc catcaatgta gaatttaaac tcgccttcgc
     1741 cccaccaata acgttctagg caggtaagcg caaggtaagt acccacgtaa tagccttgac
     1801 cctttaatcg atcaattaac ttataatcct cctgtaattt ggtaatgcgt tcccgatgcc
     1861 aatttgcgtg aaaatatagg ctattttctt ccaattcgtc caccaaagcg taatttatcg
     1921 tataaaaaaa tgaggacata tccttcggat gtaaattctc gatcgtaatt ttagcggatg
     1981 tttggaaagg catttcgaaa taggaattaa agccccctgt aggattaacc actatcggca
     2041 acgagttcac ctcgcaacgc tctccaaacc cattacaaaa gaaatcccct aaaggacttt
     2101 caacagcagg gctatccgac ccatcccaat aaattcttaa gattaaatca cgtaaaacaa
     2161 aactgccaaa ttctgttcga tcacgaatcg tcatccacat atgacgaatt attcccgttc
     2221 cagtaatatc cgcaatcaca gtttcttcac cagaagtcag cggaattgcg ccacgacctt
     2281 tgcggcttgg tcctagctcg cttggcaaca tcgcagcttt acctttctcg cctgttggat
     2341 tttcgggagt gattgctcga ctctggattt ttttaaattg ggtaatattt tttagtatac
     2401 tcatcttttt ctcctttgaa tgtgaatcgt ttttcccttt tctacgtgaa gaattttttt
     2461 cgtatcactt attttcaatg gcttttctga caaatttgaa atactcaact cggattgcgt
     2521 acattcaatc gaaatgaagg cttgaccgaa cttcaaattc ttccaagaaa acgttaaatt
     2581 atcaacagga tcaatttgca atactccttt tttgaatttc aatccgaaat attgactgat
     2641 cattaggctg acaaaaagac ccgatgccca gccgcatttg ccgcagccaa agtctcgcac
     2701 gacttctcct tgttgagcct ctaagcgata cggccaccac caccacgaac cgtctaaatc
     2761 tgccaaacaa attagttctt ctaatcgggt tacgaaagct tgagggttgt gcagctcgct
     2821 cattaaaaca gtgataaagc caggaaaagt ggcgccggat tctaatcccc aagtgatgcc
     2881 tttgatttcc tcgctatagg ttggattgaa agaactagca gaaaaggcca tggtgttctg
     2941 atataaatga tctttattat ctaaaaattg ataaaaaggc atcaacgtag tgtctgattc
     3001 ttctccatca tgcatcaata aattaatttt tccatccaca atcacatcac ttaaaaagat
     3061 taacccttgc tcaagaatcg gtttttgata gtgcttcact gaataggttg ttttctcctc
     3121 atcaccgatt ccctccaaaa attgctcacc aaaaggacca tcgatagtca tttgtttaca
     3181 aattgattcc tttagtttac aggcaatgtt tttgtatcgc ttgctaagac tcagttgttc
     3241 tagggcttca aaaatttctg caagtccaat acaggccttc caaagacaaa gattgctgcc
     3301 ggtatgatac tttccaagtg caaaggcgtc tgataaccaa acagatggaa aaagcattgg
     3361 ttcccctgac cgacgttgag ctaatatgtc ctcaataatt cttgaagcat tcagtagaac
     3421 ctgcggacac cgctcaaaga attttgtatc cccagtatgt tcaaaataaa gactagccag
     3481 taacgcactg gataacgaat tactcagaga atgattgata cctcctgtaa attttgaacc
     3541 aggaaactta acgccatatt gatcaaacca tagaaccgtt ttttgacata actccggttc
     3601 aaacatggtg aatggcaatg atgcgtaata catatcctta ttccaaattc gattagtagc
     3661 aggatagctg ccccaattgc tgccgacgac ctgtccctgt cgattcatac cgaaactaga
     3721 aaagctttga taaagtgcac gacgatagag atggacaact ctatcgtttg gcattgacag
     3781 ctgcccaaat agctgagcaa aatagttcaa tgtttccttc agccattttt cgatatcaga
     3841 ttcctgaaaa gtcattactt cttcaaaagc atttgggtcc acaaaagcaa tggcgaagct
     3901 tgccacttcc tgttgtgcga tcgtgtcctt atttcctttt tgagaaatca acacattttg
     3961 ctgatccgaa tacttctctt gatacgaagg cacatctttc actgaaactt gaaggctttc
     4021 gtgtgtttca ttcacaatgt aaacctgttg aacaagcata cttaaacgct tattagccag
     4081 tatgggagca aaggatacta caatgacctt aaacgtcgaa taatgatgaa tatatctagg
     4141 caaacagtct tgaatcacat cgacttcaat acgatgatcc gattctgaca aatggtagac
     4201 aatcccgttt acttcaacct ttaaggataa cgaattggtt tgagagagct gtttattgac
     4261 ccaaacacct ggtttttcct cagttagtaa attttctcga taaaaggtca acgtttgaat
     4321 cgtcccgcaa acatcaaggt ttgctaatgt cttttgatta cttacatcga aggtcaatcc
     4381 ctttacatca ggaatattcc gatagtagct gtattcgttt gaagtagtca catttccgtc
     4441 gtttaactca gcattaaaaa atgggtgttt gggtgaataa attacttcat taatagtcat
     4501 cttttccctc cttgctaaaa aaactgcagc ctagtagctg cagcttccta ctatgcgatt
     4561 ccaagtaatg acaataaaat tcccaagacg accattccaa aaataatttt gatgacgccg
     4621 acttttttat ctagtaattt gaaacagctc aatgttacca ataacggtac aattccttta
     4681 aaaatactat cgaggatttc ttggacattc attagttctt cgcccttcat tgaaaagctc
     4741 aaaacggtat tgaatttgac catactgatc gtcattgcac ctaccatgat cagacccaag
     4801 atactagcag cttttgtcag aatttcaatt aacccgttcg catacagctt ttcaatatat
     4861 ttagagccta atgaatagcc taaaacgccg ccatagtatc gcatcaagta attaggtata
     4921 ttgaacataa ctaaaaagat aattggtgcc aaaatatttc cggcattggc taagttgatc
     4981 gctagtcccg cggtaacgac acgccaaact ccccagaaca atgaatcgcc aattccagcc
     5041 aatggtccca tcaaactcgt tttgatcgcg ttgatacttt ccacgtcaaa accagctttt
     5101 tcagaatttt ccttctccat cgatgccgca atccccatga caaaggacgc gcaaggaatg
     5161 gtcgtcgaaa aatactgtgt atgacgttcc agcgccgcag ctcgatcttc ttcacttttg
     5221 tagaaatgat taatgaaggg catcatagaa taggcgaaac caccgccgcc catcgtcacc
     5281 ggattcaccg aagcatacat agtcatcgag cgccaaaaca tcttccgaac caattttttc
     5341 tcatctttag aaattccatc agctaatttc attgaaagaa atcctcctct tcttggtcaa
     5401 gcgttgcatt tacagcagca tcgctttgaa ctgcatgaac agatatattt tttagttgta
     5461 gatctctttg ggctataaca attgcaataa tcgttccaac tgcggcaatt gcaatcaatg
     5521 gtaactcgag atacgccata acaacggccc caaagaaaaa ccacacagca atatttttac
     5581 tccaaagcat ccgtaacagc atcgccatcc caactgccgg cagcagattt ccagcgactg
     5641 ttaatccgtt catgatgacc tctggaatat tatccaagac gcttttgaca acatctgctc
     5701 caaataaaac ggcaaagaat ggaataagag cataaagcgc atagtggaca aaccacataa
     5761 agaagtgcag attgatgatt cctttttgat caccagattt tgcaaatttg aaaaatttag
     5821 cagccatcgg tccacataca aaagaaaaaa tcatattcat taactgtaat cctaatactg
     5881 ctagtggaaa gacaatggga ataatcgcat ccacgtttcc tcccattttg attgcgaacg
     5941 ctgtagccag cccagcagcc aaagaaggct ccgccgtact agagattccg atattgacga
     6001 cacccataaa gatggcttct agtgacgccc cgacaatcaa ccccgtctgc aaatccccca
     6061 tcaacgctcc tgctaacgga gcaacaacaa tcggacgact cagcatggac atcccgaaca
     6121 tttcatgtcc agcaactcca ataaaaacgg ccaaagcgac tacaaatgca tcatacataa
     6181 tttttctcct tcctttttct aaaaatcact caaaatagag atagctgaac gtttttccat
     6241 cgccggtgta ggctgaaaaa cggtatctgg ataaagttca actaattctt ttaagtcttg
     6301 aatttctgac gtcgttaaca tcacttcctt tgataaggtt ttcttatctt ccgatggcgg
     6361 tgtcattttc ccagcattcc ccacgttcat taattcgatt tccgttggga aagctttcgc
     6421 aacttttacc gcatctttga cattagggac taaaaccatc aagcgcatgt ccttcgcccg
     6481 cggatctttt aacaaatgaa tcgcgtcatc aactgtttta ataatgactt tgacattcga
     6541 tggtgcagcc atcttcaatg tcatcttttg aatatcgttg gtagccgcct catcgcttgc
     6601 tactactaac ccggttaaat ttagactctt tgtccaagtc atcgctactt gtccatgaat
     6661 taatcgttca tcaattcgca ctgctttaat catttttctt cctcctaaaa aaaatcttcc
     6721 tcttcattta ctacatgtac ctcatcaaga atggtcatct ttaattcctg ttggcattta
     6781 tttaccagtt cctgaatatc ctcctttgtt aattccttgg ttgccaatac tatttcgagc
     6841 agaattggca gattaaagcc agacaaaata gttacagcaa tctctttctc caaaaaggtt
     6901 ttggtaatct tctgattgac acttccgcca aataaatcgg taaaaacaaa aatctgatcc
     6961 tgctcgttca cctggtttac aaatacattc aactcctgtt caaaatcaca ctcatccaca
     7021 taagcattaa tcaccgtaat ctcttgaacc ttccccgtca gtaattgaat actgctaaga
     7081 aagcctgaag ctaagtgacc gtgagtcgct attagaattt tcttcatatg gttaattcct
     7141 ttctttctgt tcacttttat ataagcaatt cctgtgccaa cttttaaaaa cattggttta
     7201 atcacgttaa ttcacattaa aaaaagtgtc taacctaaaa aaaacagatt agacactttt
     7261 ttagacactt tatttttgaa tatacggtcc taataattca tagagcagcg aaagctcata
     7321 agcattgact ttaaaattat atttcatctc cattgattga aaaatacttc tagaaacata
     7381 ataaaattca tcttcatctg gtttcaaatc ataattcggt tcttcagatt tttctgtccg
     7441 agccaagaat aaacgttcca tcatcaatgc cgtatgcata tagagattta gttttacacg
     7501 gccatttaaa gtgaaatgat agtaattttc atatttagaa atgactgttt ccacttcact
     7561 catcacgatc gttgggttca gaaagttcaa acggtccgca attccttcca acgaaaagaa
     7621 ttttactagc tcctgaatca agcggttaaa ttctgccggc ttcatttcag gactcaaaat
     7681 atgctccaaa ttggctgctc catcaccgtc taagatgtca taaacattaa tattaggaat
     7741 tgaaaaggaa cgtggaagat ccgaagtcgt gatgaccaat tttgtcttgt caaaataagt
     7801 ttcatcattt tggtcgatta attcacgtag ttctctgtag tccttcgtga aaacttcgat
     7861 ggattcatcc gataaatgat gacgaaaaac atctttcaat ttgtccgaaa tccccaaacc
     7921 cgacatacag gaaatgataa tgttgctact ttgagaaaag ccttcaaagt attgaacatt
     7981 gattgaatac ttgtctgctg catcctccgc aatctgttta aatggcgcat tcatcgtcat
     8041 cttcaagcca atatcaagtg caatggaagt cgtcaaatta ttcagaagaa gtaactcccc
     8101 ttttaaatcg ttcttgatct gtgtgtaaat ttgatttaaa gagcccatat ctacgatcag
     8161 cactagacta tctgtattca cttgccgttt cacgaaattc cttgtcgatt cgacaatctc
     8221 tgacacatta ctctctactg gcatatccaa cgcttcaaaa acataattgc cgcataattg
     8281 attcactact gcttgaatgc tgctagccgt actatctccg tgagcaagca acaggccctt
     8341 cagctcgatg gattcatcta cataatcaga taaagtcacg acaataagca gtcttagaaa
     8401 gttctcgtcc ttttctgaga gcggtggtaa atgctccaag aactgattac ataagtaagt
     8461 cgtccgaggt agatttgcag ctaatgtacg atttatcccg gttaagtctg tcttatagct
     8521 tggggaaatg acataggatc tgctaataat ttctgcaaca ggtccactcg tcgtcaatcc
     8581 gtacgttttt cctaaaacgg atacccagac ctttctaaac atctgaatca tcaattttct
     8641 actcattgat aaaaaagaat ccttatcaat gtgatgcacc aaatgctgta cttcattctt
     8701 tatcgaagaa aaattttcat tgaaattacc ttcatttatc agctgagtaa ttttttccaa
     8761 aatcgaatca taatcaaacg gtttgattcg atccgttttt atcttttgat ttttctttat
     8821 caataaacta gcaatcggca agctttctac ttcggattcc agcatattta actgaatcaa
     8881 gatctcattt ttcccaatcg ttaatggttc ttcttgtgtt cgactataca catccgcaca
     8941 actgatttta atgatattct tcagcttacc aatattccca ctaaagggat gagataacat
     9001 taaagacaag gcttctcttg aaacaataat atctttttgt aaaacatccg cttcattttg
     9061 aaaaaataat tgaatcagct ctaatcgttc tcgaatgggg cgatcttcaa aagttggcaa
     9121 cataacacta atttgaatac gccgatcaaa ggtgtctaat aaataatttt cacctttttc
     9181 cgtcgtggcg aaaataaaac gaaccttagc agaatgccaa tttacatttt caccgactgg
     9241 acgatagttt ccagtatcaa taaatagaaa gagcttttct tgattttctt tcgataggcg
     9301 atggacctca tcgagaaaaa gatagccgtt gttggccgtt gccaataaac catcggtatc
     9361 cttttcagcc cccgtaaagg cccccttctt gtatccaaac agtgttgccg aaagcagttc
     9421 gggattgtcc gcataatcgg cacagtttaa gatagcaaat ggtgcatctt tttcaataat
     9481 ttgctcatgg atagcgtatt caaaaatttt tgtggctaaa aaactttttc caacacccgt
     9541 ggctccattt attaatacac tgagtccgtt aggcggatat ttgacagcgg ctatacattt
     9601 ttgaatgacc tctctcagac ttccgtcatg accaactaca gaatgaaagg cttcgtttat
     9661 ttgctccgta ttatctacaa ccgcatattc atctaaaggc ttccacaaaa caggctttcc
     9721 cggtaatttc tcgacctttt tatcttccac taatctcgtc aaataatggc tggcattctg
     9781 acgagagata cctagattct tcgaaatttt ctcggcagtg acagaagaca tattccctgt
     9841 tagtcgtatc agctcatcaa aaactcgatc tttagcgctc tttttcattt tttcctccag
     9901 tttaatgtgt ctaaaagcat aataaactat cggctgcaat atgtctattc tatatttgta
     9961 ttccaatttt tactttgaaa cacaaatagt cgaatatttg cctcagatat ctgacaaaat
    10021 aatttatcta ccaatttagt ttttatctaa tatgattttt atgttttttc atagctacaa
    10081 atattgaact ggattcatat attagtttta ttaatagatt acagaagggt taagtatatt
    10141 tgcatagttt ccaattactt tatccttctg taactttttc aatcttagat ttactagccc
    10201 gtctcgggcc ctattttaca atggttttgt ccacttttct ctggtagaaa aacgtggtag
    10261 aaaaaataaa ataacacaaa aaaagcggcg tttcatccgc ttttggtaga agattggaaa
    10321 actcagatag aaaaaggacg atttttagct ctttagaacg ttgataagtc cccattctta
    10381 ctgagttttg ccattatctc ctatacatct tgaattctaa gaataggtca ttatagatgc
    10441 ctaggtattt cgatcctaga tctttgtaga cttcaagatg ggagatcgtg tcatcagatg
    10501 gatagaattg ttcgtccgac gaaatgtctt ttggcaataa cgcttttgct tcttcatttg
    10561 gtgtagaata accaatatac tctgcatttt gtgccgcatt ttccggttct agcataaagt
    10621 tgatgaaatc ataggcacct tctttatttt tcacggtttt tggcataacg atattatcaa
    10681 accaaagatt ggatccttct ggtgggatca cataatgtaa atgttcattt tcccacatca
    10741 tatcggcagc ttcgccagaa aatgtcactg ccacggaggc ttcttcattg atcatataca
    10801 ttttgatctc atctgccacg attgctttga catttggtgt taaatgattc aatttatttg
    10861 ctgcttctgt cagctgtgcc atattcttac tattcaaaga atagcctaaa ctattcaaag
    10921 aaagccccat aacttctcgt gcaccgtcaa tcagcatcaa actgtctttt aattccggct
    10981 tccacaaatc atcccaatgc ttaatttgat ttgcatcaaa gaatttatcg ttatatacga
    11041 tccctaaagt tccccaaaaa tatggaatcg aatactgatt atctggatca aaatccaagt
    11101 ctaaaaatcg tggatcaata ttatttaatc ctttgatctt cgaatgatct aatggaagta
    11161 ctagtttttc tttgatcatt ttttggatca tatattcact tggtatagcg atgtcgtagt
    11221 ttgtcccacc ttgctggatt tttgtgaaca tcgcttcatt agaatcaaat gtttcataat
    11281 tcactttgta tccatattct ttttcaaatt tcgtgatcaa actaggatcg atataatctc
    11341 cccagttata gatggtaagt acctttgctc cactcatgcc acttgttttt tcaagttgac
    11401 gaacaccaag cagcagcacc gcaataatga tgagaatacc taaaaacaga gattgtaatt
    11461 ttttcatcgc agccgtgcca ccccctcgcg tactcttctg tttttcttca gccgttttgg
    11521 acggttttct ttactaataa agtagtatcc aatgaccaat atcatggaga acaagaaaac
    11581 taacgcactt agcgcattga tttctaaact gattccttgt cttgcacgag aatagatctc
    11641 tactgagagc gtcgtaaagc catttcctgt gacgaaaaat gtaaccgcaa aatcatcaag
    11701 agaataggta aatgccataa aatatccagc tatgattcct ggagaaagat aaggcaaaat
    11761 gatatttttg actacttgga aattattagc accaaggtct ctagccgcat cgaccattga
    11821 atcgttcatt tcctgcaatt ttggcaagac catcaagacg acgattggaa tgctgaaagc
    11881 aatatgggaa agcaatacac tctgaaaacc taatccgaat cctagtagtg taaagaaaat
    11941 caagaaacta gcaccgataa taacatcagg agagactaat aagatgttat tcatgctcaa
    12001 caacgctgtc cgtgcacgtc gttttttcgt gtaatagatt cccatagccc caaatgtccc
    12061 gatgatcgtg gcgatcaatg cagataagaa agccagcatg aacgtattca atacgataat
    12121 gatcaaacgt gtatcttcaa aaacagcttc ataattttcc caagtaaatc ctgtaaagct
    12181 gttcatcgtt cctccttcat tgaaggaata aaaaatcaga tagaagatcg gtgcatataa
    12241 gaataaaaag acgatcccta aataaagata cgaccattta aattttttca tttatgagcg
    12301 acctcctttt ttcttctcgc ctgtcaggag catcacgatg aacatagcaa tgattaaaat
    12361 cacaccgatt gtagaaccca tcccccagtt ttgtgtaacc aagaaatgct gttcgatagc
    12421 cgttcctaaa gtgatcaccc gatttccgcc aatcagacga gtcagcatga ataaagaaag
    12481 agaaggaata aatactgctt ggacaccact tttcacacca tttaatgata acggaaaaat
    12541 cactcgtcgg aaggtctcaa agctattcgc gcctaaatct ctactagcac tgatcaaaga
    12601 aggattcatc tcttccaatg cattgaaaat tggcataatc ataaatggaa tctcgatata
    12661 cgttgccacg aacagaaaac tgaagtctgt gaataatatc tgtttcggcc cgatcccgat
    12721 aaaatcaaag aaatgattca cgctgccgtg cacactgaaa atcccaataa atgcgtaagc
    12781 tttaagcagc agattcaccc atgtaggcaa aataatcagc atcagccata attgcttgtg
    12841 cttcagtttt gttaaaaaat aagcagtcgg gtaactgatg atcagcgtga aaaacgtgat
    12901 caaaaatgca taccatacag aattgatcgt catagaaagc gatgtccccg acgtaaaata
    12961 agatagataa ttattcagcg tgaattgccc attcatatca aaaaaagatt gataaatgat
    13021 cattaataca ggcgcgatca cgaataaacc gagccacaaa atataaggga cggaatagat
    13081 cctgcgcatt gttttcattc ttgtcccccc tattcttcgt agctgtcaag acgtgcatcg
    13141 aattcttctt cagactcgtt gaaacgcatg acatggatat cttctggctc aaaggccaat
    13201 cctactttgc ttccttcttt agcttttttc gttgaatgga ccatccattc attttgctgt
    13261 tcatcataac agatgatttc ataatggaca ccacgaaaca gctgagtatc gaccgttacg
    13321 actagttttc ctttatctgg tgtcgtgatc gtcaaatcct ctgggcgtaa aacaacttca
    13381 actggttcat ttagacgcat cccgccatcg acacattcaa attgcttacc aacaaactca
    13441 actagattat cttcgatcat tacaccgtcc acgatattgc tctccccaac aaaatcagca
    13501 acgaaatgat tgattggctc atcataaata tctacgggtg taccactttg gacgattttc
    13561 cctttattca tgacaaaaat ctcatcactc attgccaacg cttcttcttg gtcatgagta
    13621 acaaaaataa acgtgattcc taatcgttgt tgcagatccc gcagttcata ctgcatatct
    13681 gtacgtagtt tcaaatcaag cgccgataac ggttcatcta atagcaatac tttcggttca
    13741 tttacaattg ctcgtgcaat cgctacacgc tgtctttgac caccagacat ttcactaatc
    13801 tcccgttttt cgtatccagg aagctggacc atacgcaaag catcttttac cttcttttcg
    13861 atatctgatt tggacatttt cttgatcttc aatccaaaag caacgttatc aaaaacattc
    13921 atatgaggaa aaagtgcata atcttggaag actgtattta cttgtcgctt attagctggc
    13981 aaatcattca accgtttccc ttcaaaatag atgtcccctt ctgtagcttc ggtgaatcca
    14041 gctataatac gtaggatcgt tgttttacca cagccggaag gaccaagcag tgtataaaat
    14101 tttccttgtt cgatctcaaa acttactttt tttaatactg tttcatcatc atagcgtttc
    14161 actacttgat caaatgaaat gattggtttc cccacgtttt taccccctat attacaaata
    14221 tgattgcgtt gcaacaatca atacttttgt tcgttttttg agatgattcc ttaattgatg
    14281 ttgttttgtc gcttgataat agatcgactg tcctgctttt gccacataga ccgtctctcc
    14341 taagcttaac tctaattccc cttctaatac aaaaataaac gtttcagata atgaaggttc
    14401 aaacgtctta tattctcctg caccttcaaa agtcagcaag acaggctcca tttctttttc
    14461 attcgaatca gccacgagcc attccaattc atacccgttt tcttcatcga aataaaccgt
    14521 atgatcttct tttgaataaa ccacttgcag attcgctgct tcttgatgaa aaaattcttc
    14581 tggagtcaca cctaagactt caagaataga aaagaatgtt tccattgatg gtgaacttaa
    14641 atctcgttcc aactgggaaa tatacccttt tgacaaatct gtgcgttcgc ctagttcttc
    14701 ttgtgtcaaa ttcttttgga tgcgcaaatt tcttaacttc tcaccaattt ccataatttc
    14761 ctccgaatcg aagttttcta atagtaaact tcctgtttac taccactcgt attagtatac
    14821 gaaatttatt gaaaaaggca accctttgta aaattttttt tgctgaatat ttctttatct
    14881 tttggaataa attctagttg tttgattctt tatttttcac ctaatttttg tctaaattta
    14941 cggaagaaca agaataaaag agtaggcttt atggtatgat aaaagaaaat tttaaaagga
    15001 gtgtctcacg aatgaacgct tttcgatctt ttcaggtaat aaaggaacct gttttccata
    15061 cagcaatcag tgatctgcca tttactgagt tggctgaaaa tgaagtcttg gtaaaaattg
    15121 cctattctga tgtcaattat aaagatgcat tggcaatgag cgagtctggt caggtgatac
    15181 gaacatatcc gatgactcca gggattgatt tatccggaac tgtcgtccaa agtaacaatc
    15241 cacgtttttc aaaagaagat cttgtacttg ctactggttt tgggcttggc gtcacccatc
    15301 caggcggtta tagccaatat caaaaagtac caggagactg gttagttcct ctaccaaaaa
    15361 atatgacgct gcgtcaagcg atgatccttg gtacagcagg gtttactgct atgttgtgtg
    15421 tgaacgcttt aatccgtcaa ggtatgacct ccgataaaaa agttgtcgtt actggtgctt
    15481 ctggcggtgt aggcagtaca gcgatagccc tgttacataa actaggattc cagtcaatca
    15541 ttaccttttc cagaaaagaa gagtctgtca catggctaaa aagtctaggt gctttgcaag
    15601 tcgtacgacc tgatgaattt ctgccagaga caacaaaagc tttaggaaaa cagcaaatcg
    15661 actatgtcat cgacacagta ggtggcgagc agctaagcag tcttcttcca ttgatctctt
    15721 ataacggtgc agtagcttta tgtggcaatg ctgggggaat caaactaaac gcgactgtct
    15781 tgccttttat tctgcgaaac atccagttga ttggaattga ttctgtcaat gtaccaatag
    15841 atcaacgtct ttctttatgg caacagatgg cggatttgca gattgcagat gaactggtcg
    15901 tccaagagat tactctggat caattgcctg aaacagcatc taaactactt gctggaacac
    15961 accaaggaag aacactagta aatgtaggtg accataaatg aagattctaa tcacagctgg
    16021 aggaacgact gaaaaaatcg atcaagtacg tgctattaca aatcattcga caggacgatt
    16081 agggcaagca ttagctgatt atttagctgc caatccggat accacaatcg attacgtgac
    16141 tacgaaacaa gcactgaaac cgaaaagaca ctccaatata acgatttata cgatcgaatc
    16201 tgcactggac ttatttttac aattagaaga actaacgaaa aaagaacact acgatgcgat
    16261 tatccacagt atggcagtca gtgattttac tcctgctttt agtttttcag aagaacagct
    16321 ggcaaaaaac ctacccactt cttcaacgca agaagaactg gacaattggt ttgcggaaaa
    16381 tgaacagaca gataccactg tttcaaagat ctcttctaac accgagcatc ttgtgctcgt
    16441 tttgaagaaa acaccaaaaa tcatttctta tctacgtgaa tggcagccaa aagcaaaaat
    16501 cattggtttc aaactattag tagatgtacc aaaggaatct cttctggcag tcgcaaaaaa
    16561 tagtctgata aataacaaaa ctgattttat tttcgcaaat gatttgacgg aaatccatgg
    16621 cgagacacac cacggctatc tcttatctaa agatggaaca gtggaagaag ctcaaagtaa
    16681 atctgagatt gctgcattga ttactgagaa aattagactg gaggaatcaa aatgaaaaag
    16741 atcttactag gcgttactgg aagtatttct gcttacaaaa gcgctgatct tacaagtcaa
    16801 ttcgtcaaat taggtgccca ggtagatgtg attatgtcta aaagcagcac acattttatc
    16861 acgccattaa ccttgcaatc tctgtcgaag cgcgcagtcc atacagacgt catgcaagaa
    16921 tcaaatccgc aggtaatcaa tcatatcgaa ctagcaaaac aagctgactt gtttctcatc
    16981 gcgcctgcta cagcaaacat tatcggcaag ctagcaaacg gcattgcaga cgatcttctt
    17041 tcaacagtcg caatggcgct tccagtggga acaccaaaat tgattgcccc tgccatgaac
    17101 acgaatatgt atcaaaaccc gatcactcag cgtaatctgg aaacattgag atctgtcggc
    17161 tatcaggaaa ttgaaccaag agaatctctt ttagcttgtg gagattttgg aaaaggtgcc
    17221 ttagctgata ttcagacgat catcgaacag gtcatgctga ctcttaatga aagaaatgct
    17281 tagaaaaaag gagtacttat gaaaaataca aaaaacttta cattaactgc gatgttttta
    17341 gctattttga ttttattagc tgttacgcct ttaggtttta tccctatcgg tccaatcaat
    17401 gcaacaacca tgcatattcc tgtaatcatt gcttcgatcg ttttaggacc aagactaggt
    17461 gcttttcttg gcggtacatt tggactgatc agcatgatcc gtagcacagt tatccaaaca
    17521 cctttgtctt ttgtcttttc tccgtttatc ccagtcatcg gaacagaaca tggaagctgg
    17581 aaagccttgc tgatcgcgtt tattcctcgc atcttgattg gggtggttcc ttatttcgtt
    17641 tataaaggtc tgcgcaaact aatgaaacaa aaagcagatt ccgtttcttt attcttagct
    17701 ggactttccg gttcattgac caatacgatt ttagtgatga atatgatcta ctttttattc
    17761 cagcaagatt atgccaaagt catcggaaca aatttaaatg ctgtttattc agctattcta
    17821 gcggttattt ttacaagtgg tatacctgaa ggaatcgtcg caggtcttgc gactgcagca
    17881 gtatgcaatg tcttgttaag atacttcaag cacccttctc ttactaactg atagtcatcg
    17941 tagccgatct ctgatttttt tctcttttgg tgttactatt gaatggaaag atttgacgtc
    18001 gaaaggatga tccaaatgaa aatttttgtt gtaggtgcaa acggacaaat tggccgacac
    18061 ctgataaaag atttagctcc aagttcccat gaaatttttg caggggtgag agatgttgct
    18121 tcccaaacat tagtaaaaaa agagaatgtt tcgtatgtct cttttgattt aacttggtcc
    18181 gtggaaaaaa tgaccgaagc attcaaaggc atcgatgtat tgatttttgc tgccggctct
    18241 caaggaaaga atcttctgca agtagacttg gatggcgcaa tcaaaacagt gatcgcagct
    18301 gaaaatgcgc atgtctctcg ctatctgatg gtcagtgctg tttatgcaga tgaaccagca
    18361 aaatggcctg agtccatgac agattattat attactaaac attatgcaga tgaatggctg
    18421 aaacgaacta acttagattt tgtcattctt caaccagtca cattgactaa cgatgaagaa
    18481 gttacttcca ttcaattaac taagcctaat gagaaggcat caaaaacgat cactcgctct
    18541 acagttgctg ctgtacttgc cgctctagtg gaagaaacgg atatttctcg tacgactcta
    18601 gtactttctg aaggtagcaa agagttgaat acagcattcc aagaatgggc aaaggaggaa
    18661 tagatccatg cgtgccattt caatcaagca tcctggttct gtagatgtat tagaacaaat
    18721 tgaagtgcct gttcctaaac caaaacaagg cgaattactg atcaaagtcc atacagcagc
    18781 tgtcaatcga acagatatta tgaaaagaga atcagtctct ttgaaacctc cctaccctat
    18841 tttgggagtc gaagtagctg gagaggtagt tgagaataaa agtgatatct ccacttttac
    18901 tcctgggaca aaagtatacg gattagtcaa tctaggtggt tatgccgaat atgctgtgat
    18961 gcctgccgat cgtgcaatcc ttctccctga tacgctagac tatgtttctg ctgctggaat
    19021 ctcagaagtt tttttgactg cttatcaaac actttactgg cttggaaaat tacaagaagg
    19081 cgaaactgtc ttgatccatg caggagcaag tggtgtggga acagcagcga tccaattagc
    19141 tagacagctt tctcacgcga aaatcatcgt tactgcagga tcagaagaaa aactcgcctt
    19201 ttgtaaagaa cttggtgcag atgaattgat caactataaa aaacaagact ttcaagaaga
    19261 ggtacgaaaa atcactgaag gtcggggagt cgatgttatt ttggatttca tcggcgcttc
    19321 ttactgggaa aagaatttag cttctatagc cgttgatgga cgttgggtct tgattggtat
    19381 gctaggtggg accatcgtgc ctgagataga ccttggtgta ttgattggga aacgtgtcca
    19441 actgcttggt acattattga cacctagaag tgatcagtat aaagcagagc tcgcacaaac
    19501 ttttatgcaa aaagctgggc cttttctaga aaatggcaag attcggccaa tcattgaccg
    19561 tacattccaa ttcagcgcag tcaaacaagc gcatgaatat atggaagcaa acaaaaacat
    19621 cggaaaaatc attttgaaga taactgaata aaatcatgct gatcaaaaaa gaggctggga
    19681 cagaagcgtt taactccaag aaataagaag aaattcacga aaattgcttt tcaaattttt
    19741 gtgaatttca gcttatttcc gaaggaattt cttctgcttc cgccgtttat acgtgtttag
    19801 agcgtgagac aaaagtgttc tttacttttg tcccacgctc tttttccgaa taaacggtgt
    19861 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacataag aagctgtttt
    19921 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct tcactaaaca
    19981 gtgttcaatc agctgaccct cggtattagc tcaaaacgct gatttcacaa cctcttttta
    20041 tttacgttct atttcattca ttttccgaag caaatcgagc tgcttgtatg cccgcttgtc
    20101 gcccaaaaat aatgatctcc gcaactgaat ttccaccgat ccggttgttt ccatgtaagc
    20161 cacctgtgac ttcaccagca gcatataacc ccctaattgg ctggttttct ttatctaata
    20221 cttcagtgtt cgtattgatt ttcacaccgc ccatcgtgta atggattcct ggtgcgattt
    20281 tgatcgcata ataattcgga gacgataagt catgttccat ggctgtggca cggttgaatt
    20341 gagcatcttt tttctccgca acatccgtat tccatccatc aactgttttt gctagtgttt
    20401 cttttggtaa atcgattttt tctgctaatt cttccattgt ctttccagtt gtgacatatc
    20461 ctttttggtc ataaaattca atagctgtgg cacgatcacg cacgccttgg tcaaaaatca
    20521 aataagctga tttttcaggc aatgctgtaa ttgctgcaga tactttatcc cttgtatcca
    20581 tttcattgac aaatcttttc ccctcttgat cgaccaagat tgctccttct ccgcgaacaa
    20641 cctcaccaat caagaccccc tcatcctgct gcacagttgg atgaatttgt attttatcca
    20701 tatcaacggt ttgtccacct aatttttcaa tcatggtgat accatctcca gttgatcctt
    20761 cttgatttgt cgtaatataa tcagctaaat cgggacgata tttttcaata aattctttgc
    20821 tggcaccaaa accgcctgtg gtaactacga ttgcttttcc ggtgacctct tttggttctt
    20881 ctccttgaac atgtacagtc acttcattga ccgtttcccc atttttctta atgtctgtca
    20941 catctgcgtt gacaaaaata gggatttccc gctcatggac gttattcaat aacccctcca
    21001 ctaagtatcc accaatagcc gagccatcag acggacgatg tgtcctcttt tcactcatcc
    21061 ctccagtgat cgttaagttg tccagtttga taccgttttg atctagccaa tcgaccgcat
    21121 cagcagagtg atcgacaaag tatcgaagca tttctttatc attcgttcct ccaccacctt
    21181 ttagcgtttc ttcatagaag gcatcattcg agtctttgat tccttcttct ttttgaaatt
    21241 ttgtttctga agcattcata ccgcttgatg atttagatgt gtttcctccg gcaacaggca
    21301 tcttttccaa aattactgga ttcaacccag catcttttgc ttcgatagct gctgccatcc
    21361 ctgcaccacc agctccaata atcacgacat cataagattc tttcatttct tttggatctg
    21421 tatatacttg ttcagaagcg cctgatgata cttcggcaga ttccttcgta ctagaacttg
    21481 tctgcgctgt atttttctga tctgcgccgc atgcgctcat agcaagaagt gcaacggctg
    21541 ttaataaacc agcaactaat tttttcattt gtatccccct tttctcctta tactatatta
    21601 agtgattttt ttcacaatat caactctgtt tttctttaag tcatatagct ttggtaaaca
    21661 cacgcttctt agtataatta aggatataca tgaaggagtt attttatgaa tcatttgaaa
    21721 acctttttta cgaacagtac aaaaactgca ggtagaatca caaacggaat ctatatttta
    21781 ttacgtatca tcgtcgttat tatgctagtg gatcaggtct g
//
LOCUS       DAYYHX010000030        21813 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000030 DAYYHX010000000
VERSION     DAYYHX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.030"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..540)
                     /locus_tag="ACHA6Y_000819"
     CDS             complement(<1..540)
                     /locus_tag="ACHA6Y_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8986932.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEI"
     gene            complement(552..1244)
                     /locus_tag="ACHA6Y_000820"
     CDS             complement(552..1244)
                     /locus_tag="ACHA6Y_000820"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM8986933.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1263..1547)
                     /gene="macP"
                     /locus_tag="ACHA6Y_000821"
     CDS             complement(1263..1547)
                     /gene="macP"
                     /locus_tag="ACHA6Y_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM8986934.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1560..2123)
                     /locus_tag="ACHA6Y_000822"
     CDS             complement(1560..2123)
                     /locus_tag="ACHA6Y_000822"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8986935.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2351..3013
                     /locus_tag="ACHA6Y_000823"
     CDS             2351..3013
                     /locus_tag="ACHA6Y_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM8986936.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3137..4333
                     /locus_tag="ACHA6Y_000824"
     CDS             3137..4333
                     /locus_tag="ACHA6Y_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM8986937.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4434..5810)
                     /locus_tag="ACHA6Y_000825"
     CDS             complement(4434..5810)
                     /locus_tag="ACHA6Y_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8986938.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5847..6713)
                     /locus_tag="ACHA6Y_000826"
     CDS             complement(5847..6713)
                     /locus_tag="ACHA6Y_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8986939.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6802..7827)
                     /locus_tag="ACHA6Y_000827"
     CDS             complement(6802..7827)
                     /locus_tag="ACHA6Y_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8986940.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7845..8519)
                     /locus_tag="ACHA6Y_000828"
     CDS             complement(7845..8519)
                     /locus_tag="ACHA6Y_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8986941.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8703..9596)
                     /locus_tag="ACHA6Y_000829"
     CDS             complement(8703..9596)
                     /locus_tag="ACHA6Y_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8986942.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9670..11367)
                     /locus_tag="ACHA6Y_000830"
     CDS             complement(9670..11367)
                     /locus_tag="ACHA6Y_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8986943.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11360..12673)
                     /locus_tag="ACHA6Y_000831"
     CDS             complement(11360..12673)
                     /locus_tag="ACHA6Y_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8986944.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12673..16614)
                     /locus_tag="ACHA6Y_000832"
     CDS             complement(12673..16614)
                     /locus_tag="ACHA6Y_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8986945.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16723..17181)
                     /locus_tag="ACHA6Y_000833"
     CDS             complement(16723..17181)
                     /locus_tag="ACHA6Y_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8986946.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17479..17997)
                     /locus_tag="ACHA6Y_000834"
     CDS             complement(17479..17997)
                     /locus_tag="ACHA6Y_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8986947.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(17999..18616)
                     /locus_tag="ACHA6Y_000835"
     CDS             complement(17999..18616)
                     /locus_tag="ACHA6Y_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8986948.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18613..19467)
                     /locus_tag="ACHA6Y_000836"
     CDS             complement(18613..19467)
                     /locus_tag="ACHA6Y_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8986949.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19464..20375)
                     /locus_tag="ACHA6Y_000837"
     CDS             complement(19464..20375)
                     /locus_tag="ACHA6Y_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8986950.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20416..21750)
                     /locus_tag="ACHA6Y_000838"
     CDS             complement(20416..21750)
                     /locus_tag="ACHA6Y_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8986951.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
BASE COUNT         6336 a         4802 c         3770 g         6905 t
ORIGIN      
        1 aatttctgcg cctagtgtgg actcaaaatg tctcaatgcc cattcatgtc caacgggatc
       61 gaaacttgcc acggaatctt tgaaaatatg gagtttgtat cctagattat acgcatcgac
      121 tgctgtatgc aacacacaga tatctgtaca tacgccggtc aaataaatat ctgtgatctg
      181 gcgttctcgc aaacggatgt ctagatctgt tccactaaaa gcggaataat gccgtttgtc
      241 tatccaataa acattttcct cttgctcata gtcttggtat aaacttttta aagagccgta
      301 caattcccgt ccacccgttc ctatcacatt atgcggagga aataagcggt tctccggatg
      361 aaaggaatct tttggatcat gcgcatcaat ggcaaaaacg acaaaatcct tttgatcaat
      421 aaacttcttt gtatgagcag tcagcttttt ttcaattact tgtcctgctg tcccagtagt
      481 cagctttcca tcctctgcaa caaaatcata tgtgtagtca atcgagatca atgcgttcat
      541 cttagtcctc cttatacgag atgtttgaca aattcgatca ccatttctgc tgatcgtttg
      601 cctgcgtctt cgataaattc gtcaaagctt tgtgtcgccg agtgatctgc tgtgtcgctc
      661 attgcacgta cgatcaaaaa tgggatattg aactgacgag ctgtttgtcc aacagcggct
      721 ccttccattt cgcaagctaa tgcttcaggg aaattcgtta agatttcttt gattttatct
      781 ggcgagtcga caaatgtatc acctgtaacg atcaatcctt cttttgcgtt caaattcgtt
      841 gcttttgctg ctttaaccat ttcactgcgc aaatatgtgc ttgcttcata atatagaggc
      901 attcctggta attgtcctgg gttgtagcca aatcctgttg catctgcatc aaaataagct
      961 actttgtctg atataacgat gtcacctact tgtaagcctt caccgattcc gccagctgaa
     1021 cctgtattga tcaccatgtt cacgccgtat tgttggatca acagacttgt ggtaatagat
     1081 gctaagactt ttcctatacc ggaacgtacg acgatcactt cgtggttacc taatgaacct
     1141 gaaataaata aagcgcctgc gcgttcccat gataatggtt cgcttaggtt ctctctcaaa
     1201 atttttactt cttcttccat tgcgccgata ataccgattt tcatcttttt aattccttct
     1261 ttctatataa atgcgattgc taaaaaaacc acacataaca ttaggataac aataataaga
     1321 gatttcatca aaaaagagtt ccatttggtc gtcttttccc gttcgctgat ccttgttttc
     1381 gtgattggtt tattcttttt gctttcttta cgataaaagc tggcaatctt tttttcttct
     1441 tgctgataag ctgtttctgc ttttcgctgt ttttttaatg actcgcttgc ttgtgcttgt
     1501 tgtcgcttgc gaagttcgct tcgcgtaata agcggtcctt tggccataag ccgatccctc
     1561 tactttcctt tggtcaatag ttcccagtat tgaatcgcat aaatcgtctt tgcatcacaa
     1621 atcaactgat ctttcatcgc ttgttgcgcc tcttcaagag ttagatgata caactccagt
     1681 acttcgtctt catcttgtgc taaaggattc tcgaccttct ctaccccttg tgcatgataa
     1741 atatgaagga cttcatttgc aaaacctggt gaaagataca tcgaagtaag atggctcagc
     1801 gacttcgcac gatagccagt ttcttcttct aattctctag ctgctgtcat ttcgggattt
     1861 tgtccttctc ctggatcgat ttttccagct ggaatctcca aaatcacttt ttctaaaggt
     1921 tttcgaaatt gtttgacaag aagcagacga tcttgttcgt caaaagcaat gatcccgaca
     1981 ccgccaggat gaaataccag ttctctcttc gaagttcctc catctggcaa acgaacttcg
     2041 tctacagcaa catcaataat ttttccgcta tagatttctt tgcgactgat tgttttttct
     2101 tcaaattgct cattgttcag catatcttct cctgctttct ttgtaatcat aactgctttt
     2161 ttttcatttt tcaatttttc tcaaaaactc cttattagtt taccatagat aaaccttaga
     2221 ttttctctaa atatagaaaa tccttcttta gtccgatact ctttctagta gtccgtatgc
     2281 taaaataagt atgtaatcga ataacgaaaa gaattcaata ctcaacgata aatgacatgg
     2341 agggtttatt atgataaaac gaaattggcc gtggcttcta gcattggtct tgctatttct
     2401 ctttgtccga aacaatagtt tttcttttgt tctaggactt ttattgatag gtgtagtttt
     2461 agttttgtgg ggactctttg gttcgcgcag gaaaaaggga aaacaagaag aaattcctgc
     2521 actgactgac gaacttgaaa ctcactatga aaaaagcggt atgacagcga atgaaatttc
     2581 attttttcga caaacaatga atcaaacaaa aaatgagatc aatcaattgc aaatgaatat
     2641 gcagcagaca gccaaactta aagcaatcga tctccgccat gatccagtca aagcagcgaa
     2701 agctttattc aaagaattgg tgaaagaacc aaaccgactg catgaagcaa gtcagtttct
     2761 ttatacgcat ctgccgaatc tagttgattt gacaaataag tataatgaga tcaatgacca
     2821 tgagatcaaa aacaaacaga cctatgaaaa actagaagaa agcgcgcaga tcatcgacca
     2881 gctgtcagct ttaatcgcac aagattatca aaaattcgtt gctgaggatc tagatgatat
     2941 cgacgtagag atatctgttg cgaaacagag cttaaagcgt gataataaat atgaaccaga
     3001 acaaaaggat taactctttt gttcataaaa gaagactcgg aacattcaga tgatgtctgg
     3061 ctgtttcgag tactttttct aaagtagaga cattcatgta agaatcgaga acagattcaa
     3121 caaggaggaa ttttttatgg atcataaacc gactgaaaca aaagatgtta caccagttaa
     3181 cgatacactt gaagatttat tgaacaaccc gttttcaact ccaacagata cgctgaccac
     3241 ttcacaacaa gcggagattg atcagctcca agagcaacag acagctgctc gtttaattga
     3301 caaactaccg gcggaacgcc aagaacaagc taaacagcta gcagcaaaaa tcgatgctaa
     3361 tgatgcacag tctgtcatca gctacggatc ggctgcccaa gcaaaattaa gtgaattctc
     3421 acaatcgatg ttgaatcatg tgcaagcaca agatattggt cctgtaggag attccttaac
     3481 tgaactgatg tatcgtctcc aagaagccaa tcctgacgaa ctgcgtgccg gagaagggaa
     3541 catcttccag cgtgtcttcg ggaaggtcaa acaatcgatc tatgaagtga cagcaaaata
     3601 tcaaaagatt ggtgcacaaa ttgacaagat ttcagtgaaa ttagataaag aaaaagacgg
     3661 tctgctaaaa gataatctga tgttagaaca gctttaccag aaaaacaaag actactttga
     3721 tgcactgaat atttatatcg ctgctggaga attgaaaatg gaagaacttc aaacaacgat
     3781 cattccagaa gcgatgaaaa gagcagaaga aactggcgat cagatggatg ttcagattgc
     3841 caatgattac acgcaattcc tagatcgtct ggataaacgt acgcatgatc ttcgcttagc
     3901 tagacagatc acgatccagc aagcaccaca aatccgtttg atccaaaaca caaatcaggc
     3961 cttggctgaa aagatccaag catctatcgc tacagccatt ccgctgtgga aaaaccaagt
     4021 cgtgatcgca ttgaccttgc ttcgtcaaaa agatgcagta actgctcaac gccaagtctc
     4081 tgaaacaaca aacgacttat tgaagaaaaa ttcagaaatg ctgaaaatct cagccatcga
     4141 aacagcgaaa gaaaatgaac gtggaatcgt ggatatcgag acccttcaaa ccactcaaaa
     4201 tgatttgatc gaaacaatcc aagagacatt gcgtatccaa aaagaaggaa aagaaaaacg
     4261 tcgtcatgca gaagtagagc ttggacacat ggaagaagat ctgaaacaaa aactgttgga
     4321 tttaacccaa tagatcacct ttcgcttgtt actaaaacaa acgcccaaca acagtttatt
     4381 acaactgttg atgggcgttt ttcttatctt tcttctaact tctccattgg agtctatttc
     4441 tcattcatct tgctgtgtcc atagccatag ccaaaataaa tgatcagtcc aataaccatt
     4501 acgataccga acacgatcca tgtcacagtt tgcagacgaa gcatcaatac aatacaagtc
     4561 aaaatggaga cgattggcaa tagcggaacg aatggcactt tgaattctcc agcttttggt
     4621 ttgccaaaca tttttctcaa cttcagtaag cctagactaa caagcatcaa ataggcgagc
     4681 gccatgatat tcgttagttc cgctaataca ttcaatggga aaaatccggc aaagaataca
     4741 gctgccacac cagcggcata agtcgctttt ttcggtgtac gatgcttttg atcgatttct
     4801 gtcataaact taggaagtaa gccatccttg ctgacagcaa acagtaaacg agcaagtgaa
     4861 tacatcatcg agatcgttac cgtcagtagc gttaaaattg cccctacagc gatcaccgcg
     4921 cccactgttc catgtcctac atacctcatt gcaaaagcca ctgggtcatt gactcctagt
     4981 tcagtatacg gaacgattcc tgtcaagatc agcgtcacga tgatgtaaag gacagtagca
     5041 atcagtatcg aaccaatgat tcctcttggc acatcttttt gcgggttttt gacttcctcg
     5101 gctgccattg caacggcatc aaatcccaag aaagcaaaaa acacaagagc tgcaccatct
     5161 agcacaccgg aaaaaccaaa aggcatgaat ggctgccagt tatcaggttt aacgaaaaat
     5221 attcctacaa tgataaacaa cgcgatgata ccaaatttga catataccat ggcattattc
     5281 aaacgtaatg ctttcttggc ttcaagcgac acgatgtaag tgacgaatat cactacgagc
     5341 accgcaatca agtcaatgta tgtaccattt tccggattgt acccgccagt taatgcttgt
     5401 ggaagttcgg cccctaaact tttcaaaaaa ccttgcatat agccagacca tccggaagca
     5461 acagaggaaa ccgctagtaa aaattccccg atcaacaacc accctgtcag ccaggcagca
     5521 aattcaccaa atacaacata cagataggca tatggcccac caatcactgg cactcgcgaa
     5581 gcaaattctg cgaaacttag tccagacaaa atgacaacga ttgcagctaa gacaaatgaa
     5641 agtgacagtg ctggaccagc attttgattg gccgcaaccc ctgtcaccac aaatattccc
     5701 gtaccgatga tcgcaccaat tccaagcatg atcaaatcag acgtttttag ttcttttttc
     5761 atctcactcg tatgattgag attgatctct ttcttacgaa acaaatccat tgaagttcct
     5821 ccccatttta ggttggaaaa cagcgattaa actgttttca tataccctaa aaggatacca
     5881 cctaaaatga ctaaaaggca accaacaata acataaatga gctctttttt cgtttttctt
     5941 tcgccaagga tgaaaatccc acctaaagta gagataatga tccccatttg tgaaagtgag
     6001 aagccaacag ctaaaccaag tgatttgact gttagaagca tgcagatgtt tcctaagccc
     6061 cataataatc cacagatcat gtttttccac acaaactgat cgactttaac tttacggaaa
     6121 gcaaagaagc ttgcgcctaa gatcattccg atactttgag gtaagataat tgacatcgca
     6181 tccaaattcg cccagttgac gatgatggta tatacaccat aaccaatcgt agagaagatc
     6241 aatgctctaa aacccttacc aaaatcaagc attttattat ctgtatctat ttttccttcg
     6301 tcatcttgtc gggcagtcaa atacgcaccg cttacaagaa gagctaaagc aataataccg
     6361 tatacataat ctttcgtttg tgtccattca tggaagaaaa cggctcccgc aatcgtattc
     6421 aaaattaatt gaaaaccagt agacaatggt agccctacag aaacacccat gtacttcatt
     6481 ccatggaact gtccattttg tccaacactc caagctaagc cggataaaaa tccgatcaag
     6541 aaaatccaag gggtgattac aggactaaca acgaaaaaga caactaaaga aaacagaaat
     6601 gcaccgatcg tcattcctag tgtttgttga ttggcatcgc caccaatttt accacttact
     6661 aaaccaatac taccccatgc caacataggg actaatgcta ttaaaatttc cataaccaat
     6721 ctccttttat ttcacatgat aagaaactta tcagcgaaaa cgttaacaac acaagcactt
     6781 tttttcgttt ctaacgattt tctaacgttt ttcattataa aaatttaaga ttgggaaaaa
     6841 taaagtcatt gttgtccctt ttccttcagt tgattcagcc gtcaaatgga tacctaattt
     6901 ttcggccaga ttttttgcta aataaagtcc taatcctgtt gaatgtgttt tgctaagtcg
     6961 accatttctc ccagtaaatc ccttatcaaa gatccgtttc aagtcttccg ctgggatacc
     7021 gatccctgaa tctttaactg aaaggctgac tccttgcgct tccttttcta tgctcacaat
     7081 gatgtttccg tgatccggcg tatatttgat ggcattacta agcaattggt tgaagataaa
     7141 agcgatccat ttagcatcgg tcaaaacagt atgatcctca ccaacgatct cgtagcgaat
     7201 atttttttgg ataaaataat ttgcctgact gcgtatcaca ggctgaatga ttccttttag
     7261 tccatattct tggatgaggt agtcccgcga gaaactgtcg agtcttgcat aatacaaaac
     7321 ttgttctacg tattcatcga tttttgataa ttcattttct agtaatgtat acttttgatc
     7381 gtcaatatcg aactcgatcg aacgaactag caatttagaa gcggaaagtg gcactttgat
     7441 ttcatggacc catgagtcaa tataatcttt ttggtcttgt tgattactga ttgctgattg
     7501 cattacttct tggtgttcac ggatcagctg attgatatat tcttctacca gtttttcttc
     7561 ttctgtcctc ccaccactca aataattttg caaaggggaa gttttttgta tgtcggctaa
     7621 ttttccccac catcttctct tgctcaagta accacccagc agataaaaaa tcagaaagac
     7681 gccctcaatc aaagctaaat aagcaattgt gctcaaatca actttgatgt ttggcgccag
     7741 ccacatcaca aagatcgtca atgcaataaa aaacagccat cccaagatca atgtccactg
     7801 atctttcaaa tatttaaaaa aattcatctt aaccgctcat cctttcaggg aacgatatat
     7861 ccctgtccta cttttgtttc gatatatcct tggatacctg cctgttcgat ttttcggcgt
     7921 aaccgattga tgttgactgt caatgtattg tcatctacaa atcgttcgtc atcccacagt
     7981 gctcgcagca atttttcccg acttaagatc tttccattgt gcttcatcaa aataaataat
     8041 agacgatatt catttttgct caaatcaatg atctctccgt tgatttccat acttccgttg
     8101 tcaacattca acgtaatgcc attgtggctc atcatttcac tagataattc tgtgtagttg
     8161 taagaacggc gaagtaatgc attgattttt gcaatcagta catcgatttg gaaatgtttt
     8221 gtcacaaaat catctgcccc catgttcata gccatgatca tatccatatt cgtattcctg
     8281 ctggagataa aaatgatcgg tactttcgat acttcacgta ttttttgaca ccaataatag
     8341 ccatccaaaa cgggaagatt aatatctagt aatacaagct gtggttcttc tctttcaaag
     8401 tctgagaaca cttgattgaa atcggtcgtt ccaaaggttt caaattgcca tttctcaagt
     8461 tcctccatga tcaattgtcg aatgatctct tcgtcttcga ctaccatgat ttttgccata
     8521 aaaaatccct ccatctttct tcttgctact acaatatcaa agaagccctt gtgaaacaag
     8581 ttggcttacc tggaaaatgt cattttagca aacgttaaaa aaaactggaa atatgcgatt
     8641 taatcgcgtt tgtctcttcc aagcagctta aatttattat gttattctta ataattggtc
     8701 tattatctgt tatttttctt tcgttgtttt tgtcttttaa attttcgata acaccaataa
     8761 ccgaaaagcg ttagcactag caatatacct agaaggagta taagtaatcg ataaagatgg
     8821 tatttttttg tctgttctat ctttgaagag acttcttctg taaagggaac tcgtcttcct
     8881 gtcactagta atcggtgagt gttcacggtg taaggtgtac aagtgataag tgtgactaga
     8941 tcctgctttt ctctaattgc taatgtattt gtttttgttg gaaggacaac aattttctca
     9001 acgacttcat aagcgagcat ccgaccatac acttcaagat agaatcggtc atcttgctcc
     9061 atttcttcaa gatccgtaaa gagttttttc tcagcaatac cgctatgggc ggtaatcacg
     9121 gaatgtgtac tctctcctcc agttggaaaa gaggtgcctt gcaggactgt cgctcctcta
     9181 tctaacaaaa gatcattcgt ttcatcaaat aaaggcaagc taacattgat tttcggaata
     9241 tagatggctc cgatcatatg ttgttcgtaa tattcttttc ctggattttg gacatttcta
     9301 accgcttgct caaaaggatc tttgacttgt cccattcctg ggattactga tgtatgagca
     9361 agcgtctgat ttttcttttc atgcactgct aattgttgct catgtttttc ttctttatcg
     9421 cgttcaagct gcttttgata attctcgatc cttctttgat ccaaataatt gctgagagca
     9481 tctgctaaaa atggatatgt aaaaactgca gctccggcaa gaaacatcac tgccataaat
     9541 aattgtaata aaagactgat ttgggtttga ggtctattac ctattttctt tttcattttc
     9601 ttcaccttct tatttaaaaa aactgcagca acctttataa aaccgattgc tgcagcttat
     9661 ttttgttgct tatacttcag acttcatttt agatttacgg taccagctgt acgctccaat
     9721 catcaaactt attccgatta gtaagaatgc caagagcccg ttcccaccag tagcaggaag
     9781 tgacccgcgt ttcatattcg gtactctttc tggccctgga ttttctactc ctacgatctg
     9841 aatttcttca ttatagcttc cataagagat gataaattca gtaaaggctg cctcacctgt
     9901 tggaagtaca taaccttctg gcgccatggt ctctttcatt tgataggttc cataatctaa
     9961 cccttgaacc tcaaagtatc catttccatc tgcgacaaat tctgttgctt gtccgcttgt
    10021 tgcccactca attctatctg ctcctgattc ataaacagct aaaggcgact cccctttata
    10081 aaatattgcg tattcagaac ggtcttcatt ccacaattcg aatcttgcat ctttaagcaa
    10141 ttcactgctt gatgcatcat atttttcaaa ctttcttcca cctgttgtga ctttctctgg
    10201 cggctctact ccttcttcat aatcaaattc atcatttcgt ccgatttcaa taaccgctgt
    10261 gttgttatgg aaatcatccg cttctagttc tgtggaaacc ttcaaattat acgtgatcgt
    10321 taaatctcct ccacgtgcat ctgctaatct acctaaagta tctactttgg ctactccatt
    10381 ttcttctgtc aaaatcgtca cgacaaaccc gttgcttact acttctacta tataatcttg
    10441 tccttttgtt aaaatcgtct cgccggctcg cacttctatt tcctgatcat aataagttag
    10501 accaactgta ggcgtatctg tgattttgta atttttgata acagcgcttc cgttttcaag
    10561 gacagaatcg atgaaataag gaataggtgc tgtgatcgtg taagaagccg tttttcctct
    10621 ttctagatca atataagaaa tcgtttcttg ttctccagct tcattaataa tcgttacttg
    10681 tctaaggtcg ctttcctcta cattcatctg tttatctgct tctctgattt cgtttttcgg
    10741 atacaaatgc acatctgtat tgtatgtgtc tttccatgta gaaccatccc acacgccatc
    10801 caccatatca ggcatcatca ctggcatcat aacaaccatt ggagacgcga ttctggaaat
    10861 atgagctggc gaataagttt ctaagaaaag atagacttta tctctagaag aaggtgcttc
    10921 ttccgtcact tggacggtag agaataccgc ttctccagct tcattcgttg tttgtttgtc
    10981 aatacttgtc agtcgatatt ttaaaaacca actttctgtg tgccgttttt gaatccattc
    11041 aatcgcttcg gcagaattaa gaccgtcttc tcgttccggt gttaaaggat tatctcgaat
    11101 caaatcgtaa tatacttccg ttacgtcaaa caaatcaaaa tcgactcctg gaatattttc
    11161 ttcaccaaaa tccgtagaaa ccaatccgct attcggtcgt tcttccggaa cagatgagaa
    11221 accttttttg tgtaatgtaa tcgtcacttc acttggtttt tgtgcgactt cttcggcttt
    11281 gactagtttt ccgttcccga aaatccctat tcccgcagta acgagtaaca gtacactcat
    11341 tcccgtgaca atcatttttt tattcatttt tttctccccg ttcttttttt attaataaaa
    11401 cagcacctaa caataaaaga attcctagta aagaaatcaa cgttcttgct tcattcgttt
    11461 gcgggaatac ggaccatttt cctgattgac ctggattgct tggaccactc ggctctttcg
    11521 gtttgtccgt tgtttctttt acggtctcgt tataaacacg tggtgttgcg gattcatagg
    11581 cagagactgg aactttcccg gattctaatt cagccatctc ctgtccgtta actactacat
    11641 attgtggttg gccttttgca tccacccaaa attcagggat gatgacctcg attctcttac
    11701 ttgcttcatc catctcgtaa ccttccacac cttttaattc ttcaaaataa taggtgccag
    11761 aaggcaagaa acgttgtcct gtcgtaacaa ggccctcttt atccgaaatg aatttgctta
    11821 catttttgtc attcaaagga tcattgtctg ctggtttgat ccattgattt ttcaagtcat
    11881 tagcaggagc catatctaaa taatatttcc ccccttctat catctgatat agagcaaaaa
    11941 cagcaccttc taatggtttt cctttttcag ttgttcctct ttgacgtcca tacttgaaga
    12001 agtatggatc tcttagataa ccgacatttt taggatagat atgaatctct tgcagttcct
    12061 cggttgattc tgtcgggtga taaattggca aaatagcagc aattgggata gctgtctgat
    12121 cgatatcaac atccaatcca acttctggat caatcgattc ttcgaaaatc aaataaactg
    12181 catcccgatc ttggtttttc ttaggtgctt tgatacgtgc aatcccgtct cctaatccgc
    12241 caaccgtatc tttcccagtt gttaccgaag tccctatttg gacaagcctt tcttctgtga
    12301 ttagctgacg gatattgttt ctgttcgttt cagcgatttg ctggacgaat tctttaggag
    12361 tcatgttttc agcagctttt ttttcatgat aataatcggt catgtcgtaa atggcaaatg
    12421 tcgctccatt tagtggcacc gtctgtgaaa tgatatctgc tgtttcccct gcttcctcag
    12481 agatagccaa gccatcattt tcatggtatt caaattgatc gttataatcg atatctcgaa
    12541 tcatccgttt atgcaacacg aattccacga catcttcccc tgattcttct gccgacatcg
    12601 catgtggaag aaggactgct gcaagcggta gtaaaacaag taattgaagc atcaaacaca
    12661 acagcttttt cattaccttg ctccctcctt tttagattgt ctgacaaaca aaccataggc
    12721 tgctcctgca agcgccatga agaatgcagc aagcaggata tagaataatc gtccggatcc
    12781 tcctgtagca ggcaacggat tttcaggatc gttcccgaca ctaagctgta atctgattcg
    12841 atttgtctcc aaaagttcat attctaactc ttgctgtaga tcaggcgtct ctgcttcttc
    12901 gtttggataa tacgaaatct caacttttag tgtacccgct tcttttcctg gaactacccg
    12961 atcattttct atgtcaggtt cttctcgata actaatttct aaaataaagt aacctggaag
    13021 ttttctatag ctgtcaggtg acttcacttc cttgatccga tagacccctt ccaacaagtc
    13081 tgaagcattg aatagggaag aatcgtttgg atctggtgcc aagcctccct cgatgatttc
    13141 atactctcct gcactatttt gtttctcaat tgaaaattct gcgccttcaa gacgtcggct
    13201 attcatactg tcaattttca aaagttccaa atcgattggc gctagttcat cttctaaggt
    13261 tacttgtagt acaccatcga tgatttcata gccttccggc aagggagcag tttcgtcttc
    13321 gatgaccgta tcttcaatga taaacttacc ttcttcattc agtgcaaact ggaacacttg
    13381 atcagatagc cgatacccat ttggtgcgac agattccgag acctcatatt caccaataga
    13441 cagatgcaga ggttggtcgt aatttaaatc gacatacttt tctactccta cggcacgaga
    13501 atagaagtat ccgatcccag gcaaacgttt cgattcttct ggttctaacg gattaccagt
    13561 gtatgctcga tacagcggga aggaagaatc ccccactttc ctgatatcga aacctgcacc
    13621 atctaatgta tggccttcat ggtctaattt tctaactcgg atctcccgat aatcaaacgt
    13681 attgtaagca gtgaatgcta cccataaacg atcctcttca tctgttgtat acgtgagata
    13741 ttgccaattt tcttcacctg tttgcttgag ttggatagtc cctatagcat tatcgtaaag
    13801 tcgcaagatc catgtgtcat tttcatcagg ttctttgaaa ccttctgggt tctggctctc
    13861 aatgattttg tattctgcat agttggtagt ttcttcacta tccttataaa caaatgaatc
    13921 tggctctccg attggatgaa taactttctc accatccacg attacatatt tcccgtcttc
    13981 atctactgaa tagaacgtga gtctcccttt ctcttcctct acttcgttca caccagaaag
    14041 ccctttcgct actggatatc cgatttccag tccgttacca tctgtttcat aaagttcaaa
    14101 ctctgcgcca ttgattggca tattactgcc ttcaattagt ttattgatat tcatgtggat
    14161 tggcttgaat tcatttcgca tttccagttt gacatacaac tcatcatact ctgtgccttc
    14221 tatgttaggc tcttgtacaa cagaaagctt gtgatattct ccttcataat aggaattcgg
    14281 atctttacct tccggtccgc cgactctcga accagatgta tattcatcta caagctcgta
    14341 gtgacctaac atctcatctt cgttggctgc atattttttg atttggaatt gatacgtctc
    14401 atctactaac tgataacgtg gtggtccagc gatctctctg atccaaaata tgtcgctgac
    14461 tgctccagtt tcatctccta aaatcggcat cttcgtttca aagatcgcta ttccgtccgt
    14521 ggtagtttta cttgtttcga ttggttcggc ttccccactc tctggatttt ggtcatataa
    14581 tgcaaattcg actccagcca acggctcgcc attttgaccc ttcttgctga cttggaaatt
    14641 acggccttta tgttcgtaac gaatggaaga agatgggaaa tacatccgtc cagtagtgat
    14701 atcattctca taatcctcca ggttctgaat ataggccata ttgttcactg tgcaaaaatt
    14761 tccgtcttga gctcccgcat caagttttac ttggtacttg atcctgacca tttccccttc
    14821 acctaaagaa atcggagaaa atttataagc ttggttttcc cgtccgacat acgttgtcgg
    14881 agtcgccact acttctggtg gggcttgtcc atgacgttct tcatcccata tttctggctc
    14941 ttggtacttc atgccgtttt ctcctggatc tagatggtaa aactgaatat ctgccgatct
    15001 gccagctcca aatggtgaga cattaaaacc agtttcatca taaagtgtgg cttgctggat
    15061 cgttctcttg aaggtagaag agatatcctc caaggctgta acaatgtcat tcttgttgtt
    15121 cgcattgtaa tatcgcggtg ttacgccatc ttcttctaaa tcagcaatat ttttcaatac
    15181 atttcgtcca atagcatcta gtcgttgtct cccgctaacg tttccatcga ttcctaaacc
    15241 aatggagaag atacttggcg ggttctcttg atacttgttt ttcagccaat gatgataagc
    15301 aactgtgttg atcgcagatg acttttcttg cgaagtagga ctgccattcg tattgttatt
    15361 agaagaacca tcaccaaccc agtgagtcgc tttattatct ccttctttta cctctccata
    15421 acgatagtgt acatttactt catcttctgg acggttaaag tcagatgcat aggtaactgg
    15481 atgagtaaac cctgcattag aagtattccc agaaaactcc tcaaaatttc tgaacaaaac
    15541 gtcattatct tcagcaatct tatcttccat cacagtccag ttgctccaag ctccattagt
    15601 gctcgtacga taacttgact gaatcggtgc ataactgaag gtcggtgtcc catctcccac
    15661 aacgattagg attttttctg gatttctatt ttctccgcca ttgtctttat acaatgtttc
    15721 atagccattt ttaagcccta gtgtaagcgg agttcctccg attggtgtac gatttaatgc
    15781 tgtattctca cttcccgtca aacgattgat atcatttgtc aatggaaaga tgtccgaact
    15841 gatttgctca tggttattga tagctgtact attatggtaa aaattcacca taccgattcg
    15901 aatatccaga ctcgggtcag agagcagatc tttactcatt tcgtttactg cctcaatcaa
    15961 cgcagcattt ttagtttgac tattggtccc agcggttaat tcactcatac tcgctgactt
    16021 atctagaaca aaaacgatat cgattggact gatttccttt ttctcgcccc cgatgacatc
    16081 tagatagaca ttgtaaaggg ttgttgcatc ttcaggatgt agatcagatg ctgtgctgct
    16141 tggcagaaca ggttctaccc attttttgat gtacgccccg ttataatcca agtaactgtt
    16201 ttcaaaattc cgttcatttc cccaaagctg aacggtttga gctggaacgt ttctatcttc
    16261 ttcgcctgca gttgatccat tattagtagc atagtcgtaa tttcgccagt tcgctccccc
    16321 gtttgtataa tcactatcat cgatcaagtt ctctccgtca tatggcgagc ctttgtctcc
    16381 ttgatagcgt tcattgcttt gtgtccaatt tcctaacact tttgtatagt cattcgttgt
    16441 aaacttcggg tagcgatgct gttcgttttc aacttctccg ctaggataga tatattcata
    16501 ggggtcttgc gtagctgttg gcccaagacg ctccaaaata ccgttgaata actggcgttg
    16561 aatgcgctgg agtgcttgac cgctgggtac gattggttcg tccagctttt ccatctcatt
    16621 ttcttcgcta cgttgaattt cagcggaatc ttctgtatca gtttcatttt cttgtaattc
    16681 ttggttatct attctttcat caaccaccac ttgaagaggt agttaccctt cgttcacatc
    16741 ggggatcttt tctgtttcaa aaatcaatgt gccggtttct gcttcttttg aataaacttt
    16801 tccgacatac cattcttgac catcgatcgt ctcgactttt aggtcttctc catcttcatc
    16861 ataggaggat agacctgttc cacgaacgtt ttttacggta ccaatcccgg cggctgccgc
    16921 atcgattgct aaacgtattc taccttcatt ggcactttcc tttttttcaa aagccagatg
    16981 ccattcaagg gtttcttctt tttcaatata cttcccttca atttttgcat agtcctcatc
    17041 taagaatacc tcatcctcct ttgtttctgt tgactcagca gctacatcca gggtcgtcat
    17101 atgatagact gactggaaat atggtccaaa tagcaaactg acagttacta actgcaaaag
    17161 cttcaatttt aaccctttca catccttata ctcctttcaa aaatatttgt cttctttcac
    17221 aacaatcaac aaaacaatgc ccatctaatt ataatttaat ttaattttaa ttttaaatca
    17281 aatgatatcc tttttagtta aaaaacacca attatacaac atttattatt aaaattaaat
    17341 tataaaattt tataacgttc tcatttaaca aaacttaagt tattaagaca aatgaataaa
    17401 caactacgct ttcctcgaaa caaaaaaata aagcacaaat gcgcatttac gcactcatgc
    17461 tttattcgta caattaattc agataacggc agcgcatagg atgccaataa cacttcctaa
    17521 aatagctcct cctaaaatgt cagtaggata atgtaaaagt aaaaaaatcc tcgaataagc
    17581 gatcaaacaa gcaaataaaa aggctaatat tcctgcaact ggacttaatt cgaacaacgc
    17641 agctactgat aaaaaggcta gcgctgtatg tcctgaagga aaagaagaag tcttcggttt
    17701 acttgtgtaa aggacaagcc gacgattttt ttcataaggt cttttccgtc ttgtcgtttt
    17761 tttgataaaa agcaaaccaa caaaaaaata gatcaaactg ccgataagaa tggctagacc
    17821 atattctcta gtatcaggta tcaagactaa aacagcagca aggagcaacc aaataaaacc
    17881 accattagag atattactga taaatttcaa aaaattgatt tttcgactat ccgttttccg
    17941 ttgcttggcg aacttgttga taatactatc atcgatttta gcaatcaaac gcaacatgtt
    18001 attcctcctt tttaagaacc ttttcttgcg tacgatggat aaaaagcagg acttgaaaat
    18061 aaaacaagaa actgaaacct aataaagcaa aatagattgg tcctgcaaac cagaatgcga
    18121 caagaaggat caacgtgcct gccaaataga aaagcgcgct tttgaaatca ttaaaaaaca
    18181 agaaaaatgc agtaatccaa gaagcttctt tttgttccag ttgaagcaag attttgtaac
    18241 taaagagaag cgcgcttaat agaagcaaaa acaacgtagt atacatcaat gtatagccaa
    18301 ttcccccacc ccaatagcgg aaaatctgat aatcgatcaa tagaagaaga aaataaacac
    18361 ttgctaaact atcttgcttt ccataaagag agaagttctc acgaaacttt ttccagaata
    18421 gtgaaccgag ctttttctct tcattttttt tgtaatgata aatcatatac agaactgttc
    18481 gacttgcagt tcctaatgtg aaaacaggca agacgcacag gaaccatagt agctgtaaat
    18541 ggataagacg tggaatccat tcaatgattt ctaagaaata atcattggct tttttaccaa
    18601 tcgatcgatt catcatcctt ttgaacctcc tgtcaaatcc atcccttcaa tgaaataacg
    18661 ttgtgcaaaa aagaacagca aaatcgtcgg gatcaagaca agtgttgctc ctgccatcag
    18721 ataattccat tgtgtcgttg cttggttctg aaaaacttgc aagccaatct gtaacgtgta
    18781 tttttcttga tcttggatat ataacagagg acctaagaaa tcattccaag ctgcgttgaa
    18841 ggaattgatc gcaatggtaa tcaaggcagg tttcgtcaat ggaagcatca aatgactcca
    18901 aatatacaaa tgattcgctc cgtcgatttc agcagcttcg atcaattcat tattgatcga
    18961 taagtaaaat tgacgcatca gaaatatatt gaacgcattg ccgaagaacg atggaacgat
    19021 caggggcaaa taagtaccta cccagccgat cttggagaat aatacatatt gagggatcat
    19081 cgttacaaac cccgggatca tcatcgtaga aagaaccaaa gcaaacatca gctttttccc
    19141 aggaaaatca agcttcgcaa atccataagc aataaaagaa tttgaaagga cattaccgat
    19201 cactactagc acagtgatga ataatgtatt tcttgcatag cgccaaaaag gaaaggcggc
    19261 aatcgtatcc agatagtttt cccatacgat tttttctggg aaaaaggtcg gaggataacg
    19321 aacgatttct tccattggtt tcaatgaagt aaacaccatc caaagcaatg gaatgatcaa
    19381 aatgattcct attactgtca tgaggacata acttagtatt ttttttgttt gccgattcac
    19441 actggtcttt ttgaaataat ccatcatttg ccgtcacctc cagaataata gacccactta
    19501 ggtgcaagct tcaaattgac aaacgtcaga accaacacga tcagaaacag gatcattgac
    19561 atagccatcg cataacccat atcaaaagct acgaacgcct tgttccacat atgcagatta
    19621 taaaaaagca aagaatttgc aggtccgcct gttccatttt tcgtcatgac ataagcttct
    19681 tgaaaaattt ggaaagaacc gatcgtagag gtgatcacat cgtaaaaaat aataggagtg
    19741 attaatggca aagtaatact tttgaacttt ccccaaacgc ctgctccatc gatttctgct
    19801 gattcatata aagatctagg aacattttgt aatgtagcta agtaaagtag cattgctcct
    19861 ccagaactcc atattttcat caaaatcaac gcaggtttcg tccattgtgg atcaaacagc
    19921 caagctggtc cagggatatg gaaccagccc agaaatgtgt tgatcaatcc tgcagatgga
    19981 gaaagcagct gcatccataa gatgtagact gctactcctg ataagactgc cggtaaatag
    20041 aaagctgtac ggaatacact gattcctttg attttttgat tgagtactgt ggctaaaaac
    20101 acactgaaaa tcgtcgtcag cggtacattg aagatgacga aataaacggt gttgtataac
    20161 gatttccaaa ataaatcatc gtgaacgaac attcttttga aatttgctag accaatgaaa
    20221 ttcatcttgg atgtcaaatt ataatcggtg aaactaccaa taactgaaaa aagcataggc
    20281 cctagcataa ataccaagaa cccgataata aaaggcgaga taaataaata ggcccatctt
    20341 gcttcttttt tctttcggat agataaacgc ttcaaatttt cttgcctcct ctgtacctag
    20401 acagtcaaaa atggattatt gattttgctt gactaagttt tctactgctt tttgtgcatc
    20461 tgccaatcct tcttttggtg attttgttcc taaaagaatc tggtcaatct gttcattcac
    20521 taatgtcgtg taatccggag catctaaagg aactggtgtg atcaccgtat ttttaaagtt
    20581 ttcgtccgcc atttgataga tcatttttcc tttatccgtt aggttgtcat tattcactaa
    20641 attctcattg gctgtcttgc tcgccatgat atcaaaactt ttttcgccga atttttcttg
    20701 tacttctgga gaagaaagat atttgatgaa ttcataagag gcctcaggat cttttgctcc
    20761 gtatggtact tccaagacga atccgccacc ccacgaccaa tggcctgaac cttcttcttt
    20821 ttctgggagc tgcatcacgc caaaatcaaa atcatccggt gcattttcca tcaaactaga
    20881 gtaataattg atattttggg cacgcatggc tactaatccg gaaataaatg gatcagatac
    20941 accagaccca aattcggctt ctaatttatt gatcgtattt ttcccataat aatcttgcca
    21001 gtcttggatc cactccaagg cttccacttt atttttagta tcaatcttga ctttttcatc
    21061 tttgtcaaac caactagtcc cgttatctgc gtttagcgcc catacatcag cccctaaatt
    21121 ccataatgga taaaaaccga tgcgtgtaaa ttcttttcct tcttttttat ctagtttacg
    21181 tgctgtttct tctaattcat cccaagttgt cggtagatct tgttcactga ttccagcttc
    21241 tttaaataac tttttattat aaaaaatgac ttgtgtatct gtattgaatg ggatcgcata
    21301 aggatcacct tcatattcta ctgtatccca aagctgcgga taaaaatcct tagaagtgtc
    21361 tttttctaca tatttggaga gattagttgc ttgctgtgct tctgctcttt gggcaacgga
    21421 atttatgtcc tgtacaataa catcgggtgc atttccagct gttattgcag ataaagattt
    21481 agtccagatg tcaccccatg gctgatactt ataatttaca tggatctcgt tttgtgattg
    21541 attgaaatcc tcaatgattt cttcgatagc ttcttttcgt gcaccggatc cccaaaagga
    21601 ccagaaatca atattttttc ttccatcact tgttttttca gaagaatttg ctgcacttga
    21661 actggcatcg cttgcattac ttgatccgca tcctcctaat aaaaacaatg ctgaacttgc
    21721 cagcaaccct gtaaacaata attttttcat attttacctc caaattttaa tgtaaccgat
    21781 gccggtttta atataacgat atcattttgt tct
//
LOCUS       DAYYHX010000031        21414 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000031 DAYYHX010000000
VERSION     DAYYHX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.031"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..1060
                     /locus_tag="ACHA6Y_000839"
     CDS             68..1060
                     /locus_tag="ACHA6Y_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8986952.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1120..2541
                     /locus_tag="ACHA6Y_000840"
     CDS             1120..2541
                     /locus_tag="ACHA6Y_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM8986953.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2534..4033
                     /locus_tag="ACHA6Y_000841"
     CDS             2534..4033
                     /locus_tag="ACHA6Y_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM8986954.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4072..5466
                     /gene="uxaC"
                     /locus_tag="ACHA6Y_000842"
     CDS             4072..5466
                     /gene="uxaC"
                     /locus_tag="ACHA6Y_000842"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8986955.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            5625..6308
                     /locus_tag="ACHA6Y_000843"
     CDS             5625..6308
                     /locus_tag="ACHA6Y_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM8986956.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            6310..7146
                     /locus_tag="ACHA6Y_000844"
     CDS             6310..7146
                     /locus_tag="ACHA6Y_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8986957.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            7157..8911
                     /locus_tag="ACHA6Y_000845"
     CDS             7157..8911
                     /locus_tag="ACHA6Y_000845"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8986958.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(9187..9528)
                     /locus_tag="ACHA6Y_000846"
     CDS             complement(9187..9528)
                     /locus_tag="ACHA6Y_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8986959.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(9596..11767)
                     /locus_tag="ACHA6Y_000847"
     CDS             complement(9596..11767)
                     /locus_tag="ACHA6Y_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8986960.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            11978..12835
                     /locus_tag="ACHA6Y_000848"
     CDS             11978..12835
                     /locus_tag="ACHA6Y_000848"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8986961.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(12906..13763)
                     /locus_tag="ACHA6Y_000849"
     CDS             complement(12906..13763)
                     /locus_tag="ACHA6Y_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8986962.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(13748..16450)
                     /locus_tag="ACHA6Y_000850"
     CDS             complement(13748..16450)
                     /locus_tag="ACHA6Y_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8986963.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(16463..17752)
                     /locus_tag="ACHA6Y_000851"
     CDS             complement(16463..17752)
                     /locus_tag="ACHA6Y_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM8986964.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            17902..18948
                     /locus_tag="ACHA6Y_000852"
     CDS             17902..18948
                     /locus_tag="ACHA6Y_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8986965.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            18950..21103
                     /locus_tag="ACHA6Y_000853"
     CDS             18950..21103
                     /locus_tag="ACHA6Y_000853"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM8986966.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         6323 a         4338 c         4079 g         6674 t
ORIGIN      
        1 cttatcgtag ttatcgtaat atgatgaatg cgttaacaaa aagtgaaaaa gtaaggaaaa
       61 tgatggaatg gtaacgatca aagatatcgc taaagcagct ggcgtttctc atacgacagt
      121 ttctcgtgct ttgaataaca gcagtttaat aaaaaatgaa acaaaacaaa aaatacgaca
      181 actagctaag gaaatgaatt attcgccaaa ctatagtgca aaaggtttag tgaatcaaaa
      241 aaaatatcta atcggactat ttttctcaag tttgcataaa ggaacttcat caaatttcct
      301 agcagattcc attgcaggca tccattcagt attagacaca aattacagtt tatctgttga
      361 aagtatcgat gttgttgatt taacagaaat caacctgcaa cgctacgatg gactgatcgt
      421 tatgagtcaa agtgatgatg atcagccttt catcgactat ctcaaagaaa aagaatttcc
      481 tttcgttgtt gtcaatcgtc acatcaatga tccagcggtt atcaacgtca ctgccgatga
      541 tgcggccggt gttatgcagg cagtagatta cggtattgga tgcggacata cgaagatcgc
      601 ttatattggc ggaaagaaaa attttcgttc gacaaaagaa cgggaaaaag gggtattgaa
      661 aagtctaaaa aaagcgagca tccctgtcaa tccagcctat tttttaacag gagattacag
      721 cttagaaagc ggcctccaaa acatggaata cctattatca ctttctgacc tgcctactct
      781 ggttttttgc ggcaacgacg acatagcgat tggtgcattg agagctgtac atttagctgg
      841 cctccgtgtt ccccagaaca tttctctgat cgggttcgat gattctccag tcgtctccta
      901 tctcaatccg ccgcttacca ccgtacgtaa gcctttgaaa gaaatgtgtc aagaaggaac
      961 aaagctttta ctagatttga tcgaaggcag gacgatcgaa caatctaaaa ttgagctctc
     1021 caccttgttg atccttcgtg agtcgatcaa ttacttgtaa actacaagta attttatata
     1081 aaaatgttaa cgtgtgcata acacagaaag gaagtttata tgaaaaaact gacaaaagaa
     1141 ttagtgaaaa aaaacgaagc acctgtaaaa gttcttcagt ttggtgaagg caattttatg
     1201 cgtggattta ttgattggca gattcagcag ctgaataacc aacaattgtt taaaggtaat
     1261 gttgcaatca ttcaaccttt agaacaaggt cttggaaata tgatgaacca gcaagataat
     1321 ttatatacgg tcattttaca aggactcagc aatggcgaaa tcgttgatac ttccgagatt
     1381 atcaccgtag tagaacaaat gatcaatcct tatgatgaat gggatgctta tctatctcta
     1441 gcagaaaatg acgacttggc ctatatcgtc tccaatacaa cagaggcagg aatccaattc
     1501 gtggagactg ataatttaac agatactccc ccacagagct tcccgggcaa actgacagcc
     1561 tttttatacc gacgtttcaa attaaacaaa gctggcttta ccatcattcc ttgcgaactg
     1621 attgacagaa acggcgaacg tctaaaagaa atcattttac gctacgcaga aaaatgggat
     1681 ttagaacctg cttttgttga gtggttagaa aaagaaaatg ttttctgttg tagtttagtt
     1741 gatcgcatcg tcccaggtta tcctcgcgaa gatgcaggtg ttttcaatca acggcataac
     1801 tatgaagatc aattgatggt caaagcagaa ccattcatgc tttgggtgat cgaagggcct
     1861 aaagaattgg agaatcaact accattcaaa caggctggct tgaatgtcat catcacagat
     1921 gatatgactc cttatcgtga acgtaaggtg catttgctta atggaccgca tacagcgatg
     1981 gtccctcttg ctttgttggc ggaattggaa actgtagaag aagtaatgaa agatccttta
     2041 ttctccgcat atgttgatca gttattcaat ctagaattga ttcctatgct ttctttacct
     2101 aaagatgaat tagccattta tgctgaccaa atcaaagaac gtttcttgaa tccttttgcc
     2161 catcacaaat tagaagcgat ttcattaaat agtgtttcaa agttcagcac ccgtctgctt
     2221 ccagtgttca agaagtatat cgaagaacaa aatcaggtac caccgttaat cactgtttca
     2281 ctagctgcgc tacttcttat gtatcgaggc gatcaagtaa agccacatga tgatgaaaag
     2341 acgataagcg aatttactga tgcttggtct gataatggaa cagctattcc tcgattattg
     2401 caaaacgctg cgctttgggg agaagatttg tctcaaatac cgaatgtgac agatacagtt
     2461 caagaaatag ctgcaaagat cgatcaaaaa ggtgtccggt cagtattaca agaaatgaaa
     2521 ggaacgatgg tccatgcttg aaaccctgca accaacaatg aaattaaatc gacgagacag
     2581 tgtggtcgtc gcattaaccc ctattccaaa acaaacacaa ctgacgattg atcagcaaac
     2641 gatcaccact cttgaagata tcccgcaagg tcataagatt gcattgggtg atttgaaagc
     2701 tggagacaac gtgatcaaat atggctatcc aatcggtcat gtgacgacag acgtaactgc
     2761 cggtcaatgg ctgcacactc ataatgtaaa aaccaatctt tctggtgaat tggattatcg
     2821 atatgaaaaa gatgtccatc caagtcatta cctttttgag aaccgaacat ttcaaggtta
     2881 cttgcgaaaa aatggaaaag tagggattcg taatgatttg tttttagtac ctaccgttgg
     2941 gtgtgtgaat ggcattgctg aactgatcgt caaacagttc aaggaaaaac atccagatct
     3001 gggacagttt gaccatatta cgattttaaa gcatccttat ggctgttcgc agcttggaaa
     3061 agatcatcaa aatactcgag aaattttagc ggacgcagta aatcatccca atgccggagg
     3121 agttttagta tttggattag gttgtgagaa caatacagtt cctgaattca aaaaaatttt
     3181 aggtgaatat gatgaagaac gagtgaaatt tctagtggct caagatgttt acgatgagat
     3241 cgaacaaggt gtggctctat tagaagagct tttaacagcg gcggaaaatg accagcgcat
     3301 atcagtcccg ctctctaagt tgaatgtcgg tttgaaatgc ggcggttctg atggtctatc
     3361 gggaataact gctaatcctt tacttggtgc attttctgac tacttgattg ctcaaggagg
     3421 cagcacgatc ttaacagaag tgccagaaat gtttggcgca gaacaagtat tgatggcacg
     3481 agcggaaaat gaagaagttt ttgactcaat cgttgacttg atcaatgact tcaaacagta
     3541 tttcttgtct tacggggaac cagtttacga aaacccatct cctggtaata aagcaggtgg
     3601 gatcactaca ttagaagata agtctttagg ctgcacgcaa aaatcaggaa gctctcctgt
     3661 cgttgatgtg ctacaatatg gcgaaaaaat cagaaaacca gggcttagtt tattgcaagc
     3721 tcccgggaat gatcttgtag ctgcctctgc tcttgcttca tccgattgcc agttggtctt
     3781 gtttaccaca ggaagaggga ctccgtttgg ctcatatgtc ccaacggtca aggtatctac
     3841 gaatacaact ctttttgaac gcaaaggtca ttggatggat tttaatgcag gcgtcttatt
     3901 agaacaacca atggaagtaa tcttagaatc gtttgttcaa aaaatcatcg ctgtagctag
     3961 tggagaagaa acgaaaaatg aacaaaatga cgttcgagaa attgccattt ttaaaaacgg
     4021 agtaacactt tgattttcat aaaacagtat aaaaagaaag aaggaagatt tatgttttta
     4081 tctgatgatt ttttattaaa agacgattgg gcgaaaaagc tttaccattc ctacgcaaaa
     4141 aacatgccca ttattgatta tcactgtcac ttagatccaa aagaaattta cgaaaacaac
     4201 aatttcagca atctgacaga agcttggtta ggaggcgatc attacaaatg gcgcttgatg
     4261 cgagcttgcg gcgtgcctga agaaaaaatc acaggaaatg cttctgattt tgaaaaattt
     4321 ttagcttggt gtcaaaccgt accaaaaatt atcggaaatc cgctttacag ttggtcacat
     4381 cttgaattga agcgtttctt cggtattgac ttgttgataa atgaagaaaa tgcagagaaa
     4441 atttggacac aagcaaatga aattttggca gctcctgatt ttcgccgtcg ggaaatcgca
     4501 aaaaaatcga atgtagaagt gatttgtaca acagatgatc ccgcagacga cttgcactat
     4561 catcagttgc ttgctcatga agaaccagat ttgaaggttc taccttcatt ccgtccagat
     4621 aaagcactca atattgaaaa agaaggattt aatgcatggg tcacactact ggaagaagca
     4681 gctgaccaaa cgatcaccac ctatcaagaa ctgatagaag tcttagggca gcggatagaa
     4741 tacttccatc aacatggatg tcgtgtttcc gaccacgctt tagacatctt acgctataaa
     4801 gaagcggatg aaacagtatt agaagagatt tttcaaaaag cacgagctaa caaactatta
     4861 tcttcagatg aaatcgatgc ttatcgtaca gaaacactga tacatttgac ccacttctat
     4921 cattctcata attggacgat gcagctgcac atccatgctt atcgtaactg taatacacaa
     4981 atgttggaaa aattagggcc cgataccggc tatgatggaa tgaatgatct ttctttgact
     5041 attcctctgc aaaaattatt gaaccgtgca gaagaaacgg atcagttgcc aaagacgatc
     5101 ctttattcat tgaatccgaa tgactatcct gcgattttag ctttgatggg ctgtttccaa
     5161 aaagaacaga atggaaaact gcagttaggt tctggatggt ggtacaacga tactcgagct
     5221 ggtatgcgcg atcaattgac acaatttgct gatggtagtg ctttggggaa ttttgtcggc
     5281 atgctgacag attcaagaag tttcctgtcc tacactagac atgaatattt ccgtcgtgtt
     5341 ctttgtgaat ttttaggcga aatggtcatg cgaggagaag cacctgaaga tgaacaactt
     5401 ttaggaagtc tagttcaagc gattagttat acaaatgcta aaaattactt tggctttttt
     5461 gcctaattga ttaatagtaa cagtaacgaa tcaaaaacag gatagtgaaa taacggaatc
     5521 cgtttatcca cgttttagag aagaagacgg accaaatttg ctccgtcttc ttctcttgct
     5581 gttctgcttc attgcctata aacctttatg aaagaagaga taacatgcct actatccata
     5641 ttgccggtga ttctactgcg gcagaaaaag atgaaaacaa gcgtcccgag acaggttggg
     5701 gagaaaaaat ctctgcttat ttttcacccg agatccagat caataaccaa gcaaagaatg
     5761 gacgcagcac caaatcattt atccaggaag gaaggttagc ccgtctagct tcacagtttc
     5821 agccgaatga tttcttattt gtacaatttg gacataacga ccagaaaatc caagaagaaa
     5881 gaggtacgct tcccttcggt agctatttaa aaaatctgca gatatttttt gaatgcgcaa
     5941 aaaaagcgaa cgtccaaatg gtcttactga catctgtgac gcgacgagat tacttagaaa
     6001 acggaacatt gaatccagat atacttggag actatccaaa agcgatgcgt gcatttgctg
     6061 agaaacacca tattcctctt ttagatgtgt tctcaagatc acaagaattg ttccagacct
     6121 tctcaaaaga agagacaaaa aaattctacc tgcatcttat acctgatact tgtaaaaatt
     6181 acccagaagg actaaaagac aatacccact ttagtccaga aggcgctgat aaagttgccc
     6241 gaataattgt tgaattgata aaagaatccc gtctgcctct tgctaattat ttacaaaaag
     6301 gagtttgaga tgtttccatt aaatttaggc atccgtgccc acgatttaga tgttcgaaat
     6361 cgtgaagaaa tcgttcaaaa agtatcacag cttcacttgt cccatatcca atttgcacca
     6421 caaaaagcat ttcctgaaca acttaaatta actgacatga cacttgggag tgctgcctat
     6481 tttggtgaat acttccaaaa aaacaatatc gaactttcca ttttaggatg ctatatcaac
     6541 ctatccagca aaaatcaaga agttcgtgca caagcattgg caactttcaa acagcaaatt
     6601 gctctgtgct ctgcctatca agccaagatg attgctacgg aaaccggtag tgtctctgtc
     6661 ggttacacaa aagaaaattt tactgaagaa gcttatcaga tcgccagaaa ttctatcatt
     6721 gaattagtcg ctcatgcaga aaattttggc gtaactgttg ctgttgaagc agggatcaat
     6781 catccaatct ataactatca gttagctaaa cgtttaatcg atgaagtagc ttcaccaaat
     6841 ttaaaaatca ttttagattg tgcgaatttg atgcataaag aaaatcatgc tgatcaggaa
     6901 aaagtgatcc gaggtgcctt ggacaattta catggttata ttacagcgct tcatctcaaa
     6961 gactatatca tggaaaatgg aaagatccgc atggtacctg ttggcaaggg ctgtttagat
     7021 ttccgcccgg tactgcatta tatcaaatat gagcgtcctc taatgtacgc cactcttgaa
     7081 gctactcctg agatttatgt tcctgaagca atagaacact tgcagtttat ttaccaagat
     7141 tgttaaggag aagattatgg aggaacgaat acagaaaagt tatcaaaaat atcatgcaaa
     7201 tgcttttcag acattgctgc atctttatca gggaaattat tggcgttttg ccatgtcagg
     7261 actctttttt attatcaaac atcttccatc ttggttaatg ccgatcgcta ttgccaatgt
     7321 aatcaatgca gcttcatcga aagattcttc tcagttgcag gccatttatt tcaacgcttt
     7381 tttgatggct tgtctgattg gtcaaaatat attgacgaat tattttcatg taaaatacca
     7441 tagcacttcg atccgtcaag tagaagctgg actaagagcg acccttatca agaaaatcca
     7501 agaactatct attccttatc aaaaagaatt gcaatctgga cgaatccagt caaagatcat
     7561 ccgtgatgta gaagctatcc aaacgttgtc ctctcaagta tttgtcagcg gattaaatat
     7621 cgctgtcaat ttattcgtag ctttaggtat cacgctttat aaaagtcctg ttgttttcat
     7681 gtttttctta ttgactgttc ctctcgccgc attcgttact ctctttttcc gtaaaaggat
     7741 cagacagtcc aactcagact tcaggcaaga aatggaagag acttctgcaa aagtgatcga
     7801 agtgatcgaa ttagcaccga ttgctcgagc tcatgggtta gaaaaacacg aaacaactcg
     7861 cttaaataag cattttactc aaatctatga aaaaggattc cacttagata tcctgcaatc
     7921 tgttttcgga tcagtcagct gggctagttt tcagatattt cagctgattt gtttgatttt
     7981 cactgggata ttagctttac aagggcgcat ccaacccggc gatgtcgtga tgtatcagac
     8041 gtatttcacg acgatcgtca attccgtttc tggaatgatc acgctgattc ctacgatttc
     8101 aaaaggcatg gaatctatca cttctattgg cgaactgttg attgctggcg atgtagaaac
     8161 ttatcacggt aagaaaaaaa tccaacattt ggatggaaat tttcgttttg aggatgtttc
     8221 ttttctttat ccggactcac tcaaaccgat tatcgatcat ttttctctag aggtgaaagc
     8281 tggagaaaca attgcttttg ttgggccatc tggctctgga aaatctacac tccttaactt
     8341 attgattgga tttatccagc cgacttcagg aaaaattttt ctagatgatc agcctttgga
     8401 tgaactggat atgcgaagtg tgcgaaatta tttagccgtt gttcctcaaa ccactttgtt
     8461 gttctccgct tcgatcaaag agaacatcac ttatggcttg aaaaacgtat caaaagaacg
     8521 tttagatgaa gtcatcgaag ctgcgcaact ttcttcactg attgaccagc ttcctgatgg
     8581 tctggatacc ttagtaggcg aacatggcaa caagctgtct ggtggccaaa agcaacgtat
     8641 ctccattgca cgagcgttga tacgtcagcc aaaaatcatt ttgctagatg aagcaacctc
     8701 agctttagat aaccagtcag agaaaaaaat ccagcaagcc ctgaattacc tgacagaaac
     8761 acccactact tttattgtgg ctcatcggct ttcaacgatc aaagaggcag ataaaatcgt
     8821 tgtgatcgat gaaggaaaaa tcgttgaaat aggtacttac gaaaaactgt tagctcaaaa
     8881 aggctatttc taccgcttga aaaatgaatg acaaaaaata ggagctatga caagactttt
     8941 gtcgtagctc ctatttctga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
     9001 caatagtcat caaacatgag aagctgtttt ttgaaattgt tccttgtaac tcagagttaa
     9061 acggtaggct cacgacctct tggtaaatgg tgttcaagag acagcctatc ggcaataaga
     9121 agcaattttg tcatcgctca aggccgaacg tctctttcac accctctgtt gtttcataaa
     9181 atagttttat tcgctgtaaa ggtcgcgaac tggtgtcatg atgatacggt tcaagtcatt
     9241 gatgattgta ctgaagcctt gctctttttg catcaagtca ttgattactt cttctgattg
     9301 gattttcaca gccatttctt gtgctttcat tgcatcttcg tcgctgaact cttgtccact
     9361 catttgtttt tcttgtaatt cgatttgcat cgcttggaag tctttgaaca gtttgtgtgc
     9421 ttcttcgttt gcttgtactt tctcgtacgc ttctttcaac gctttgaatt ctggtaattc
     9481 acggatctca cgttcgattt gattcgcgct atcgtagatg tttgacatgt tatgccctct
     9541 tttcttttag tagttactct ctattgtaac atggggttat tcgttttcaa aggggctact
     9601 ctccttgata tttccgccag aggtctttag ccgcttcacc aattccctga acgccttctt
     9661 tcaccatatc gccgaagttt ttcagccctt cttttgcttg ttcaccatat tgattcaagt
     9721 tatcttgcca atttccagac tctccattag aatttcctgt gtttccaact tgatctgcag
     9781 ctaccacttg tcctcctgtg gcataggcat ctgctacact aaatcctgtt tttgaagtat
     9841 acggaaggat gccttctgcc gctgatttga agactttccc gacaacattt ccacttgtcc
     9901 cttccaaata gtgcagcttg ctggattctt cgtaacccat ccatgtcgaa atcactacgt
     9961 ctggcgtgta accgatcatc cattggtcat ttgcttttgt ggcatcgaaa ttagtttctg
    10021 tggttcctgt tttccctgct atcgtataac ctgcaggatt cgcagcagcc gctgttccat
    10081 ttgaaaatgt accaagaagc atgctggtca tttcatcggc tgtttccttt gatatgacct
    10141 gttttggttt tttgtttgtg ttatcgacga tcaccgctcc agtcgaatca acgattttcg
    10201 tgataagatg cggcgtatac atctgaccat cattggcaaa tgcactgtaa gcacccgcca
    10261 tgatcaatgg agatactcct ttttccagtc caccaagggc taagccataa tacttatcag
    10321 attttgctaa aggcaggccg aattcttctg ctttatcata tcctttttgt aaaccgatct
    10381 catgcaaaag ccatacagta ggcaaattca aactttgtgc aacagcttga tacatcggta
    10441 cttctccttg gtatgtcccg tcaaaattat gcgcatcgta ataactttgc ggttcgtctt
    10501 tcaagatact ggaaggcttg tatccagctt ccagtgctgg tgtatatacg cttaatggtt
    10561 tgatcgttga tcctggtgaa cgacgcatct gtgtagcaaa gctgaatcct ctaaagacat
    10621 gatctcctct tcgtcctact agtgcttgta caccacctgt attcggatct aaagctactg
    10681 aagcagattg gaccatggca ccatctgaag catttgctgg gaataatgca ctgttttgat
    10741 aagtagcttc taattgcttt tgataatcct gatctaaagc tgtatagatt ttatagcctt
    10801 tattcatgac atcttcttca tcaagaccat actcgttaac tgcttcatcg atcactgcat
    10861 caaaataata aggataacga taaccagaat ctgaattatc gtatgtgtca ttaagatacg
    10921 cgctgatatc tgtctggctt tctgcttgtt cttgttcttt tgtgattttt ccgttttctt
    10981 ccattacact tagtactgtg tttttccgat cattcgcatt ttttgggtga tcgatcggat
    11041 tataaatacc aggacctttc agcattcctg ccaaagtagc agcctcagac agcgtaacat
    11101 tcgttgcatc tactccaaaa tactttctgg aagcgtcttg gacaccccat accccgttgc
    11161 caaaataggc attgttaaga tacatggtca agatttcttc tttgctgtac ttcttttcga
    11221 tctcgattgc caaaaataat tccttcgctt ttcgatcaaa cgtttggtct aatgtcagat
    11281 acgcattttt ggcaagctgc tgagtgatcg tacttcctcc gccccctgat gttccgccag
    11341 acaagaccat tcggactgct gcacgggcaa tccctttgat atcaaacccg tgatgctcgt
    11401 aaaaattcct atcttctgtc gagattactg catcttggat atagggagaa atcgcgttta
    11461 attctacata tgttcctttt tgggcgtaca gtgtgccagc ttcttcgttt ttcttatcat
    11521 aaatgactgt cgattggctt aatcctgatt ttaacgtttc gacatttgct tgttttgcta
    11581 aaataaagag gtagatactt gtcactaaga cacagatcaa accaatcagc aaaatcaatt
    11641 tgttcacatg atattttttc cagatgcgct ttcgccatcg atggaattgt ccgagatatg
    11701 gtctgatcca acgccaaaaa ctgatcaaag cagcttttat cttttctaaa atgctttgaa
    11761 aatccatttg cttcttctcc tgtctatttg attcaatcgt cttattttac catagtgaaa
    11821 gagtgttttc ttcctgctta agttgtagtt caatatgttt ttttacgtta gtgcaagtag
    11881 agtaacataa gaatgaacag aaaaatgtaa gttttttctt aaatatgttt taaaaatggt
    11941 acaattagtc tgcgattgca ctgaaaggag tacacttgtg aaaatcacga tcactttacc
    12001 tatgaaccat cctgaaacaa caattcgcga cttgcttgaa aaggaatggt tagttcctag
    12061 aaaggttcga cactttttgc gtatacgtaa aaacgtttgg atcaatcaac aacctgcttt
    12121 gttccatcat gaagtaaagg gcaatgacca gatcacattg ttattcgaag aaacagatta
    12181 taattatcaa gaagttcaat taggagatgc gaagcacatc cgtgttttat atgaagatga
    12241 gcatttgatt atagtggata agcctgcagg aatgaagact catccaaatg aaccgacaga
    12301 aaacagtacc ttactcaatc atttagctgc ttatctcgac actaaacagc aacgacctta
    12361 cgttgtccac cgcttggaca aagaaacaag cggtgccgtt ttatttgcaa aaaatccatt
    12421 tgtcttgccg atacttggaa gaatgttgga acaaaaaatg atctatcgga gatatcaagc
    12481 catcgtatgg ggagaaattg ccaaagatat gaccatcacc aataaaattg gacgagatcg
    12541 ccatgatcgg agaaaaaggg tcgtagatga taaaaatggt cagagcgcct tgacacatgt
    12601 cactgtcaac aaagtacttt ctgaacaaac acaagtctat tgtgttttgg aaaccggacg
    12661 tacacaccag atcagagttc atttagctca taagggacat ccgatcgttg gagaccctct
    12721 ctaccaaaat agaacagctg aacgactgat gctccatgca ttagaactgc acatgatcca
    12781 tccatttact caagaaaaaa tagtcggtaa agctttacct ggattatggg aatagaaata
    12841 aaaaagaaca gagtcttatc agcagataat gataagactc tgttcttttt gtgtaatttg
    12901 tctgcttact tttgatgttc gtgttctgct aacagcaaac taccgataat cccactttca
    12961 ttcggcagtc cccaacgaac gatatattca cttacttgag gtgtttccat ataaccaccc
    13021 atcaattcaa caaattgctg acggattttt tgaaggagat gatcctgatt cattacccca
    13081 ccgcctaaaa taatcttttc aggtgctaac gtcaacgtat agttgatcag tgcttgagca
    13141 atataataag cctgaatagc ccaaacagga tgatcttgcg gcagatcttg ccctttgatc
    13201 cctgtgcgtg cctcaataga cggtcctgct gctaatcctt ctaaacaatc tttatgataa
    13261 ggacaagtac cttcataatg gtcatctgga tggcgtttca cccaaatatg tcccatttca
    13321 ggatgtgcga tccctgaaaa aatttcttca ttgatcacga cacctgcacc gattcctgtt
    13381 cccacggtaa ggtaaataca actatttttt ccttgtgctg ctccttgctt caattcccca
    13441 tatgcagcag cattgacatc cgttgtccaa gcagcaggaa tattgtagcg ttccttgatt
    13501 gtgccaagaa aatcgaattg tgcccaccct tttttgggag ttgccaaaac gtatccgtaa
    13561 ttatcttttg ttttgtcgat accaatcggg ccgaatgagc caatcccgat tcccttcaat
    13621 tcataacgat caaaaaattc aaatacctga tccaatgttt cttctggtgt tgtggtaggg
    13681 atacttaatt tttctaaaat ttctccacca tctgaaaccg cacacacaaa ttttgttccg
    13741 cctgcttcta ttccgccata cattcgtttt cctcctcgaa tccgattgtc cgaatctcat
    13801 aaggttggat tattgggaca accgcttgct tcgttggttc ttcaagcaga ttcaatatga
    13861 cagtttttcc attgtcttta gttatttcca atttttctaa atgactagac atattgaaac
    13921 ctcttactac tactttatca ctaaacttgc tgcgtttcaa agctgtaatt gcaaacgttt
    13981 cactttttat cgttaaatat tgttgttttg aaatcaatgt tccttcatga tgtttgattt
    14041 gttgagtgga aaaaggaatc tgtgctgcgt atgcatgcaa ataagtagag aatttttctt
    14101 ctggtccatg tagttcaatg ctgtaattga accgatgctc acctagacac tgtgcttctg
    14161 gtgttggaaa atatccccaa tcgcctagtt ctcccacact gcgaagtagc gtcactgcga
    14221 tttgtccgtc ttctgttact tcatattcat tcaaaccaaa gtttccgact gttacaccgt
    14281 actcttcatc atgaatattg acaaatgctt gctgatgttg cggattcgtg gggttttccc
    14341 aacttttaga cacatggttt gggcgcttca ccacttcaaa aatactatct gcttcgtgat
    14401 gttccacatg taactttgta ggaaacaaga cacgtaagcg atgatctttc atctgattgt
    14461 cgagagtcgt ttcaaagtcg atttttttac tatcttttct gattgtcatg attgttttta
    14521 tttgaaggat tcgtttttct ttggatcgtt ctgcttttcg ataacgaaat tccacaacca
    14581 tctgctgttc tttttctaaa cgctcgtctg cagaaacagg gatttccatt tcctgtagaa
    14641 tacttatttt tgctatttcc ggtgtatcta ctaatacttt tacttcagag ttttctttgt
    14701 tactggatag gattggctga tcacagaaag gttgtttaaa gatatactca tttcctatat
    14761 cgccgatatc ttcaaaaata tgcagattat tcaactttcg tttgtttttg cgatcttcca
    14821 ttgtaatggt accatttttt tcaatcgtca aatgtagagg gccgttttcg atgattcgtc
    14881 cactctgatg aatcattgtt tccttttctt ctgctttcgc ttccccttct acaagcgcga
    14941 atgtttccca agaaaatgct ggcatgtttt ctaatggtaa agtcactttg atcattcttg
    15001 ccatatatgg cacacggaaa cggtctttcg gcaaatcata accaaattgg acagtttctt
    15061 cagataaaat agctggtata gtggccccac tttttgtttc tacatgatac ttacgtttag
    15121 gcaaattttc taactcttga tatagttgtt caggtattcc ttcagcgaat ctttttcttt
    15181 ctaattcgat ggttatttct gcttcaccag ttttatccat tcctgcagta tttacaatga
    15241 caaatggaaa gctgtctttt gggaatccgg tcgtatcgat tgctgcggtc agctgttcta
    15301 aggaatcttg tgcaagatac tttccaactt catctgcttt ttcaaaacga ggtatcattt
    15361 cccgatggac agaatcgact gaacaaccgc aaatactgtc gtgaggatga ttttgcatta
    15421 atgtcttcca agcataatct aattgatcat ggggatagtt tccactaact tcataagcca
    15481 tcgtcgcaag tggttctgta atattttcta gctgtcgctg gaccttggta ttccattgtt
    15541 ttaagtaaac ccgagcagat gctgtattgg ctaaagtata ccagccatcc gtttcttgac
    15601 ttgtcaattc tccttctact gagcctaagt cttctggcac atctttttga actgcttcca
    15661 gatagtcagt aaaattagaa tgaataaact cgtaatctgg aaacagttca tttgcgagat
    15721 ggattgcttt tgatatatct ttttgtaccg gttgatgatc aacaccattc atcatcaata
    15781 agtggttcgt agaggcatat tgttctgcat ccgctaattt ttgcttccaa aaggcaagag
    15841 ctgcttcttt ttctactgga atctcattcc cgttgctata ccaattggca aatagcagac
    15901 caaagattgc tgtttgatca ggtcctttcc accacatctc tgagtattgg gaagaatagt
    15961 tttctgcttc caaaacttga ttatcaaagc caattggttt tacaccacgg ccaaacgctg
    16021 ctgctgaaat accagcttgt ttcattaatt gaggcgtctg tcccatattt ccaaatgtat
    16081 cgggaaaata gcctaacatg acaggggttc cccatttacg gctttcttcc atgccaatca
    16141 gcatatttct cacgttcgat tccgagctga taagaaaatc atcttgcaag atataaaatg
    16201 gtccaattct caattttcct tcattgattg cttgttggat tgcctgtctt ttttctggcc
    16261 ttacttgaag atagtcatct aaaataatcg tttgcccatc caaatgaaaa ctgttgaagg
    16321 acggatcaac ttcaaatagc tctagtagat cgtctatcaa ttcaatcagt cgcatgtgat
    16381 gctgttcgta agccatgtac cattcacgat cccagtggct atgagaaatg atatatactt
    16441 tctttttcat ttacgatacc tcctactttt ctactctaat atcaaagtaa tccatgacta
    16501 attcgcaaaa catcatattt gcccaagaaa accattctct cgtataattt tcaggattgt
    16561 tcacatcgaa tccttcgtgc ataaggtgtg ttcctgcgtc agttgctaca agtaaattca
    16621 atatccgctc tttttcagat ttgtctgacg ttgtcattcc ttccatagct aatgcaatcg
    16681 gccaaacgta tttttctggc gtatgggagg atccaattcc ttttgcgtac tttccttcat
    16741 agaaatatgg attttcttta ctcaacaatg ttcttcttgt ttgcagataa tcttcctctt
    16801 gttcagaaca atatcccaag taaggtgcgg cgatcagatt gggcacatta ctgtcatcca
    16861 tgactgtggc gtttcccatt ccatctactt catatgcata aatcgtttgc cctttttgat
    16921 taaccgttcg tccgtgattc tgaatcccct cttggatctc tttcttcaat ttctgtgttt
    16981 gctgtacaat ttctttatca tcgagtatcg tttgaaaaat ttcttccaca taacctaaca
    17041 cgaccaccgc aaacatattg gatggtacca gatatccata acggcaagta tcatcgctag
    17101 gacggaaacc agaccatgtc attcctgtaa aagctgtttt tgcacctcgc ccgtcattga
    17161 tcagcgtatc ttcttttctt gtcgtgtctc gtacaaatgt ataaggagag tgttcatgat
    17221 cttgctctgt tttgaacacg tccaagattt ttttgatacc ttcaataaaa gattgattga
    17281 attgatcagt ttttcctgta tttttgtaaa gaagataggc caactgtatt gggtagcata
    17341 aagaatcaat ttcatatttt ctttcccaga tccaatcgtt catttttgta tgatctgttt
    17401 ggtggccagc accgttagct tcttcgttga aggcattcgc atatggatcg atattgatat
    17461 aaaaaaattg ctttttgact aacccgctga tcatgtcagc taattcctca tcttcttttg
    17521 caatcactaa ataaggacga acttgtgcag tagagtctct taaccacatc gctggaatgt
    17581 ctcccgtcaa taaaaaggtg gttccgtcat tttgcttttt gactgttgtc aacagagtat
    17641 tggcaaatgc tgctgtaaaa ttctttgccc agtccgcatg gatatcccca catttttgtg
    17701 tgatatcttc cataaatttc tgcaccgatt ttggtatatc tttataagcc actaataaaa
    17761 ccttctttct atttgatata tctttaaaga ttatacttca tacagccaat tatgtctacg
    17821 ctttcaaaaa atatcttttt atttactttt tcatccatta tggtattatt tgaatatgat
    17881 atatcattaa gaggtgttta tatgaatcaa ccattgtacc agacaatatt taatgatttg
    17941 caaacggcta ttttaactgg tgaattacct gtagacagcc aactaccaac tgaaaaagaa
    18001 ctttcacgta cctataaagt aagcagaatc acttccaaac gtgcattgac tgaattagag
    18061 caattaggac ttatttatcg cgttcgcggg aaaggcagtt tcgttaaatc cgcttctccg
    18121 gaacagattc ttgcttctcc tacaaaaaca cgacggattc tgtttcttct tccatatgtc
    18181 gctgatttgt ctgttggtga ttttagcaag ggattgaacc ctgtgatgca agataaacag
    18241 attgatgtca tgatgacgac gcttgacttt ctacaaaata aaacagcaaa agagctaatc
    18301 aatgaatttg acggattgat ttattatgct cttgatacag aacaacatct tgacttactt
    18361 tttgagcttt cattgaaaga attccctgtc gtcatattag ataaaaaaat ctatgagtta
    18421 ccttttccta ccgttctttc cgataatttc caaggcggtg cgcttgctgc ctcttatttg
    18481 atcgaacagg gacattcaaa aatcgcttat ttgtttggta cgcagactca tccacaatcc
    18541 gttcgacagc gttaccttgg ttaccttgaa gcattggaca aagctggttt gactttccac
    18601 acttcactgg atgacaaaag cgcgacatta gatgaattgc tttcttatat cactaagtat
    18661 gaagtaactg cttttgtttg tgaaaacgat gttgtcgcta tccagaccat gaatctcttg
    18721 agacagcatg gttatcatat ccctgaagat ttttcaatta tcggatttga tgatatccaa
    18781 gcagcagctc ttgttgaccc gccactgaca accatcgcac aagattttga acagttaggt
    18841 aagcttgcgg gagatgcttt ggtcacttgg ttagagacaa aagatcgacc atccgatagt
    18901 aaatttccag tttcattcat caaaagacaa tcaacaaagg agaaataaaa tgaatatcgg
    18961 tcaaatagat actagacatg caacagcgaa tcagccaaat ttctctaatg ggaactgcca
    19021 gccttatact ggcgttcctt ttgggatgaa ttattttgca ccgcaaacta cggatcaaaa
    19081 cggcagctgg tggtttcatc ctgatgaccg cgtttttcaa ggctaccgtc ttacccatca
    19141 accaagtcct tggatgggtg atttcagtca tatgctgttt acgccgatca atggaaaact
    19201 acaggagaac acccttttcc atgctcaaag ttcttaccgc ccagaagaaa gtatattttg
    19261 cccgactcat ctgtcgatct gtcaattacg ttatggcatc tgttctacat tgatacctag
    19321 catgtatggc ggaatcttat ctattgatta cagcaaaaat gacagcggtt tacttttatc
    19381 ctttccggga cgccatcagc tctttgttat agatccttac acagtggaag gaaaaatcac
    19441 acattttgct ggttcagaag atcctgattt ttctttttat tttatattga gatttgaata
    19501 tcctttaaca attgatgagt tagctgtttc caacaaaaat agcgactcga tcccgttgta
    19561 ttttgaacag acaaaaaaac aaactatccg atttgggaca tcttttatca gctcggaaca
    19621 agcagcttat aacctggaac tagaacaaaa ccaaagcaaa gagacttacc ttgctcacag
    19681 tcaagaacaa tggcagtcct attttgatcg tatacagctc acacaccatg atcaacaaca
    19741 attgcggacg ttttatcata atttatatcg ggtgttttta tttccacaaa cattttatga
    19801 aattgatcct agtgggcaaa aaatccacta tgatacgact agtcgatcta taaaacctgg
    19861 tgttctctat acaaataatg ggttttggga tacgtataaa acggtttatc ctctttattc
    19921 tttgattgct gtagaaaaat acgaagaaat gctggaaggt tttttgaaca gctatagaga
    19981 aagtggctat ttgccaaaat ggctgtctcc tgatgaacgt ggacttatgc ctgggacatt
    20041 gatcgatgcg gtcattgcag atgctgctgt aaaagatatc cgacgagact tgatgcctga
    20101 atttctaacg gcaatggaaa aaggtgctac ggttcaaagc aaacatccaa attatggacg
    20161 ccaaggaaca gaagattact tgacatatgg ctatgttcct aatcactacc acgaatcagt
    20221 caatcacaca ttagactatg cttatagcga tttttgtatt agccaagttg ccaaggtact
    20281 agaagaagag gaaatgacga agaaatatca gcagcaatca ttgaactatc aaaatatctt
    20341 tgactatgag tcaggattta tgcgttcaaa agataaagat ggcaagttca gagaaccatt
    20401 ttcagctaat cgttggggac aagattatgc agaaggaagt gcttggcaaa gcagctttgc
    20461 tgtttatcac gatttttcag gactgatcgg aaaatatggt ggcagtgaga gatataaaga
    20521 aaaactcatt gagctgtgca atcagccccc aacgtttgac ccaggaggat atggttttga
    20581 gatccacgaa atgagtgaga tggccgcgat agaatttgga caattagcta tttcaaacca
    20641 gccaagtttt cactatcctt acttgttcag ttatatagga caacctgaaa tggcacagcc
    20701 actgcttaaa cagttgatga ctcagacctt cgacgattct ccaatgggtt accctggaga
    20761 tgaggacaat ggaagtatgg ccgcttggta tatcttcaat agtctgggat tttatcctgt
    20821 cacgcctgga accggtgaat atgttatcgg tatgccatta attgacaagg ctgttttgct
    20881 tttgtcaaat ggaaaggaat tgacgattga agcaacgcct aatcggccgc agcaacaatt
    20941 tatccataaa atcaaacgga tggacaaaga atatacgaaa ttatatttta cccatcaaga
    21001 tttgctgaaa ggcggcacga tctcttacca attagggatc atccctcacc ctaagaaata
    21061 tcaagcaaaa gatttgcctt tttccttatc aaactataat taacaagagg ttgtgaaaaa
    21121 gctgtcctgc atcaagtaat aagaacagcc aagcaaaaat agatcctcat atttttcgtt
    21181 tggttggtct tattaccgca gatgcaagct tttgaacacc gtttattgag aggttgtgaa
    21241 atcagcgttc tgacctgagt attagagaga caaatggcaa aatagttcct catattttgc
    21301 cattttttga gctaataccg agggtcagct gattgaacac cgtttagtgg agaggtcgtg
    21361 aaaaagctgt ttagtttcga gtattaagaa aaattttgtg aaaatggctt tcca
//
LOCUS       DAYYHX010000032        21065 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000032 DAYYHX010000000
VERSION     DAYYHX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.032"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..405
                     /locus_tag="ACHA6Y_000854"
     CDS             37..405
                     /locus_tag="ACHA6Y_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM8986967.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            575..1006
                     /gene="mraZ"
                     /locus_tag="ACHA6Y_000855"
     CDS             575..1006
                     /gene="mraZ"
                     /locus_tag="ACHA6Y_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM8986968.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            1027..1986
                     /gene="rsmH"
                     /locus_tag="ACHA6Y_000856"
     CDS             1027..1986
                     /gene="rsmH"
                     /locus_tag="ACHA6Y_000856"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM8986969.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1991..2392
                     /gene="ftsL"
                     /locus_tag="ACHA6Y_000857"
     CDS             1991..2392
                     /gene="ftsL"
                     /locus_tag="ACHA6Y_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM8986970.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            2393..4585
                     /locus_tag="ACHA6Y_000858"
     CDS             2393..4585
                     /locus_tag="ACHA6Y_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8986971.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4611..5573
                     /gene="mraY"
                     /locus_tag="ACHA6Y_000859"
     CDS             4611..5573
                     /gene="mraY"
                     /locus_tag="ACHA6Y_000859"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM8986972.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5587..6948
                     /gene="murD"
                     /locus_tag="ACHA6Y_000860"
     CDS             5587..6948
                     /gene="murD"
                     /locus_tag="ACHA6Y_000860"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM8986973.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6974..8062
                     /gene="murG"
                     /locus_tag="ACHA6Y_000861"
     CDS             6974..8062
                     /gene="murG"
                     /locus_tag="ACHA6Y_000861"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8986974.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            8077..9297
                     /locus_tag="ACHA6Y_000862"
     CDS             8077..9297
                     /locus_tag="ACHA6Y_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM8986975.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            9453..10778
                     /gene="ftsA"
                     /locus_tag="ACHA6Y_000863"
     CDS             9453..10778
                     /gene="ftsA"
                     /locus_tag="ACHA6Y_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM8986976.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10801..12042
                     /gene="ftsZ"
                     /locus_tag="ACHA6Y_000864"
     CDS             10801..12042
                     /gene="ftsZ"
                     /locus_tag="ACHA6Y_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM8986977.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            12059..12736
                     /locus_tag="ACHA6Y_000865"
     CDS             12059..12736
                     /locus_tag="ACHA6Y_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM8986978.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12750..13349
                     /locus_tag="ACHA6Y_000866"
     CDS             12750..13349
                     /locus_tag="ACHA6Y_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM8986979.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            13430..13651
                     /locus_tag="ACHA6Y_000867"
     CDS             13430..13651
                     /locus_tag="ACHA6Y_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM8986980.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13700..14482
                     /locus_tag="ACHA6Y_000868"
     CDS             13700..14482
                     /locus_tag="ACHA6Y_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM8986981.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14503..15210
                     /locus_tag="ACHA6Y_000869"
     CDS             14503..15210
                     /locus_tag="ACHA6Y_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM8986982.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15472..18270
                     /gene="ileS"
                     /locus_tag="ACHA6Y_000870"
     CDS             15472..18270
                     /gene="ileS"
                     /locus_tag="ACHA6Y_000870"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM8986983.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(18319..19845)
                     /gene="zwf"
                     /locus_tag="ACHA6Y_000871"
     CDS             complement(18319..19845)
                     /gene="zwf"
                     /locus_tag="ACHA6Y_000871"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM8986984.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19860..20507)
                     /locus_tag="ACHA6Y_000872"
     CDS             complement(19860..20507)
                     /locus_tag="ACHA6Y_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8986985.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(20691..21020)
                     /locus_tag="ACHA6Y_000873"
     CDS             complement(20691..21020)
                     /locus_tag="ACHA6Y_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM8986986.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
BASE COUNT         7123 a         3640 c         4486 g         5816 t
ORIGIN      
        1 atacttgaag caacgataaa aaaggagttt acgttcatgg gcacattcac accacttatc
       61 ttgttttggt atatttaccc ggtaatcgtt ttgtttggtt gccaatttct tgtaaaatta
      121 ttttcgttaa atcgtcgttt taaaataaaa gcccctgatt tggcgattcc ttttttgtgg
      181 ggcggtatgc atgcattgtc tagaaataca gggacaattt ctatcttacc gtttttcttt
      241 atttctgttt tgctaatggg aatttgtatt gctgttttcc aagcatatta ttatgaagaa
      301 attatttatc cgcgttattt caagatgact tggcgtttgg tttttctgtt gacaatcgtc
      361 ttgtacgctg tcttgatcat tgtgaacata ctttcttacc tatgaaaaaa atagaaaagc
      421 tgtaaaaagt cttttaaaag gctttttgca gcttgatttt taaaaaaatg tttatgaata
      481 aaaaaagttg ttcccggatt tcccagaaaa ggtggtagaa agtgggggat tgtggtagac
      541 taattttagt tagtggagga atggggggct ttcaatgttg atgggtgaat tccaacataa
      601 tattgatgca aaaggacgtt taatcgtgcc ctcaaaactt cgtgaagaac ttggcgaaaa
      661 gtttgttctg acacgcggat tggatggctg tcttttcggc tatccgatga gcgaatggga
      721 aaatctggaa gctaaactga acgaaatgcc gttggctaaa aaagacgcac gtacttttgt
      781 gcgattcttc tattcggctg ccactgaatg cgaacttgat aaacaaggac gaatcaatat
      841 acccagcacg ttaagaaatt atgcagcttt gacaaaaggt tgcgtagtca tcggtgtatc
      901 taaccgaatt gaaatttggg atgagacacg atggcaagat ttttctgcag cagcagaaga
      961 gaactttgac gaaattgctg aaagtatgat tgatttcgga ctataagaat aggaagaggc
     1021 tgactaatga ctgaaacatt tcaacactat acagtcatgt tgaaagaaac ggtagatgga
     1081 ttgaacatca aaccggatgg cgtttatgta gactgtacac tcggcggtgc cggacatagt
     1141 gaatatttat taagccaact aagttctaaa gggcatctgt atgcgtttga ccaagatcaa
     1201 aaagcaatcg atcacgccaa gctgcgttta gcttcttatg tcgaacaagg tatggtcaca
     1261 tttatcaaag cgaacttcag agaattagaa gaacgcttga gtgagcatgt agataaggta
     1321 gacggcattt tgtatgatct aggggtttcc tctcctcagc tagatgaagc agaacgtgga
     1381 tttagttatc atcaagatgc accgttggat atgcgcatgg atcagtcagc tcctttatcc
     1441 gcttatcacg taattaatga atattcctac catgaattag taaagatttt cttccgctat
     1501 ggcgaggaga aattttccaa acaaattgct cgtgaaatcg aacgagtacg aaaaacaaaa
     1561 ccaatccaaa caactggtga gcttgtcgaa atcattaaac aagtcatccc agctcccgca
     1621 agaagaaaag gcgggcatcc tgcaaaaaga atttttcaag ctgtgcggat tgcagtaaat
     1681 gacgaactcg gtgccgaaga ggcctcttta gaacaggcaa tccggttatt gaaagtcaat
     1741 ggaagaatca gcgtaatcac attccattca ttggaagaca gaattgtcaa aagtatgttt
     1801 aaagaatata gtacggtaca agatctacca ccgggacttc ctgtcatacc tgacgaattc
     1861 caaccagaac tgaaagtcat tactcgtaaa ccaatccttc cttcagagga agagctaaca
     1921 gaaaacaatc gatcacgcag tgcaaagtta cgtattgcag aaaaaataag aatgaaagag
     1981 gagtagaatg atggcagaat taaagaaaat gcaagattac gaatatgatt tacctgaatt
     2041 ggtggatcag ccggaacaac aaatgcctga ttcttcttcg aatcaagaaa tcattcttcc
     2101 acaatcaccg gcaaaacgat taaagcagat ttctgctttg gaaaaaattg ctgtggcaag
     2161 cgtggcgatt gctattgttg ctttatgtgt tttaacagtt atgctgcgca caaatatcag
     2221 cggagctgaa aaagaaatca cgtcaataca aacagaaatc aatgataaaa agcaagaaaa
     2281 aacgagttta gaacaagaaa aaaacgaact ttctcgtact gaacggatta aacaaatcgc
     2341 agaagaaaaa ggattgtcta ttaatgacga caatttaagg aaagtgaagt aaatgagtct
     2401 taaaaataaa attcggcggt ttatggaaaa gaaaaatcta aatccgatga acaatcgcaa
     2461 aaaagtaggt atcattttat ttgctacgag tattggattg ttctttttat ttgccgtccg
     2521 tttctcgtat atcgttattg gaggtcatgt tgctggtacc tcattagctg aaaaaacaaa
     2581 acagttgtac caaggaagtg aagtagtaaa agcaaaaaga ggaacgattt atgatcgtaa
     2641 cggtgtggcg ctcgcagaag atgcctcctc ttattcaatt aaagcgatac tctcaaaaac
     2701 atatacctct ggggataaaa aactgtatgt tgaagaaaag aattttgata aaatagcaga
     2761 aatcctgcat aagaatcttt ctatagacaa aaaagatgca ctgaatattt tagaggacgg
     2821 cgcaaaaaaa gagctgtatc aagttgaatt tggtagctat gggaaaaata tctcccaaga
     2881 aaccaagcaa aacatcgaag cggacatgaa aaaagaaggt gtcgctggtc tttattttgt
     2941 cgaccatcag gctcggatgt atccaaatgg cgtcttttct tctcatttta ttggatacgc
     3001 agttccagac aaagatgaga atggcttagt tggtaaacta ggtttggagt ctgcttacaa
     3061 tgatatcttg agcggtaaag atggtaagat tatttatcaa aaagataatt ttcaaaatcc
     3121 tctgccaggt acagtggcag aagaagaaaa agctgtcgac ggacaagata tctacacgac
     3181 tttagacagc agactacaaa gttatttaga aacactgatg gatcaagtaa atgaagaata
     3241 tcagccagaa gaattgacag cagtcctgat gaaagcaaaa accggagaaa tcttggcaat
     3301 gggacaacga cctactttca atcctgaaac aatggaagga ttgactggaa aagatgctat
     3361 ctggcgtaat ttcttggtac aagatagtta tgaaccagga tcaaccatga aagttttcac
     3421 tactgcagct gctatcgaag aaggagaatt caacgagaat gaaaccttcc aatctggaaa
     3481 aatccaagta gaagatgcta cgatcaatga ccatgacttt ggtgaaaaag gtgtgctgac
     3541 catgcgtcaa gctctttcat ggtcaagtaa cgtcgggatg gtcatactgg aacagcgatt
     3601 aggcggacga tggtataact atttacaaaa actgggattt ggacaaagta ctcattctgg
     3661 attagatgat gaagtaaacg gtgctttacc tacattaaat attgtcgacc gagcaatgag
     3721 tgcttatgga caagcggtag gtgtgacgaa tttccaaatg atgaaagctt tcacttctat
     3781 tgcgaataac ggtacaatga ttcaacctcg atatatcagc aaagtggtgg atccccaaac
     3841 tggagaagag cgaacaactc aaacagaagt tctagggcaa cctttttcaa aagaaacaac
     3901 agaaaaagtc cgtgaatata tgagagatgt tgtcgaaagc gaaaactacg gtagtgctta
     3961 tggtgtatat agtgtacctg gatacaatgt atcggcaaaa aacggaacag cccaaatcgc
     4021 ttctgataca gggggatatc aaacaggaga tacagcctac ctgtattcga ttgtggagat
     4081 ggttccttct gaagatccag actatgtctt gtatctaaca atgaaacacc ctaagacata
     4141 tgatcgaatg gcattggcta aaatcgcaaa tccattgatg aaacgggcaa tggattttaa
     4201 agaaactgaa gaagattcag atacagaaac aaaaacagaa aaagtatccg tagccgatta
     4261 tcgtaatctt gaagcggacg ttgcagcagc tgatgctcaa aaaagtggac tgcaacctgt
     4321 agtgatcggt aatggtaaaa aagtacaaaa acaatctact gccaacggtg atcagttgat
     4381 ctctggagaa aaactgatcc tatatactgg tggtgataag ctgatgcctg acgtaacagg
     4441 atggtcaaaa gcagatatca tgaagctggg gaaaatcctc ggtattgaag ttagttttga
     4501 tggagatggt tattgtgtca aacaagaact tgctccatat gaaaaaatca ctgaagacaa
     4561 acttagtttt actttagaag aataagaaga gaaataggag agaaaagagc atggaatgga
     4621 cacaagcatt gattcccatt gtcagcagtt gtgcaatgac aattgccgca atgccgctat
     4681 ttattggtta ttttcaaatg aaaaagcaag gtcaggcgat tcgtgaggag ggaccaaagt
     4741 ggcacaatag caaagccgga actcctacaa tgggcggctt agtgtttctg atcggctcta
     4801 ttttgacagg aatttgggtt ggagcttggc aaaaacagct aactcctact cttttcatct
     4861 tgctttttgt attagcgtta tacggagtaa tcggctttct tgatgatttt ataaaaatat
     4921 tcaagaaaag aaacatggga ctgaactcga agcagaaatt gattggacag attctagggg
     4981 gaatcatctt ttatcttgtt taccgttctg aaggttatcc aggtgtattg aatttcttcg
     5041 gactagatct acctttagga atcatttatg gtattttcgc tctcttctgg cttgttggat
     5101 tttcaaatgc agtaaatctg acagatggca tcgatggatt agttgctggc ttaggaagta
     5161 tctcttttgc agcttacggt ctaattgctt ggcatcagca tcagtatgat gtattaatca
     5221 tttgcttaag tgttttagga ggtttactgg gtttcttcgt ctacaacaga aaacctgcca
     5281 agatttttat gggagatgtc ggttcattag cgttgggcgg acttttagcg gcaatttcta
     5341 taatgctaaa ccaagaatgg acattgcttt tgattggttt gatctatgtt atggaaacag
     5401 caagtgtgat gcttcaagtt acttccttta agcttacagg aaaacgaatt tttaaaatgt
     5461 ctccaattca tcatcacttt gaaatgtgcg gatggtcgga gtggaagatt gatacagttt
     5521 tttggttgat cagcattgtg acttcgttga tcacattatg gatcgtttgg taggagatga
     5581 cgggaaatga aaaaaatatc aacatatgaa aataagaaag tgcttgtctt aggactcgca
     5641 aaaagcggag taagtgcggc caaattatta catgaattgg gcgcacttgt caccgttaat
     5701 gatggaaaac cttttgacga aaatccggaa gcacaagagc tgctttcttt agggataaaa
     5761 gtaataacag gtagtcatcc aatcgaattg ttggatgaag aattttcatt gatggtcaaa
     5821 aatccaggga ttccctacag tcatccttta gtagcaaaag cccaagaaat gggtattccg
     5881 gtaattacgg aagttgagct agcttacgaa gtagcggaat gcccaattat tgggattaca
     5941 ggaacgaacg gaaaaacaac gacaacaacg atgacaggac tactactgaa tgcaggagca
     6001 gatcaaggaa ttgcacgttt agcgggtaat attgggtatc cagcaagtgg tgtagcgcaa
     6061 gaagcaaaat cagaagataa aattgtgatg gaattgtcta gcttccaact catgggaatc
     6121 acagatttcc gccctcatat agcggtgatt accaatatct atgaagcaca tattgattat
     6181 catggtacaa gaaaagaata tgtcaaagct aaatggaacc ttcaaaaaaa catgacagaa
     6241 aaagattatc tgatccttaa ctggaatcag tcagaactac aagaattagc gcaaagaaca
     6301 aaagcacgcg tactgccttt ctcgacaaaa gaagtgttgg aagacggtgt gtatgctgac
     6361 gattactcaa tatactataa aaaagaaaaa ataatggaaa tatctgaact tggcgtacca
     6421 ggtaagcata atgtagaaaa tgcactagct gctatttctg tagcaaaatt gtatggtata
     6481 tcaaatgaag ctatacgaga aacgttgcat tttttccatg gcgtaccaca tcgtacgcaa
     6541 tatgtaggag aaatccaagg cagaaaattt tataatgatt caaaagcaac caatatttta
     6601 gcgactaaaa tggctctaag tggatttgaa acgtctaagg tcgtgttatt ggctggcgga
     6661 ttagacagag gaaatacatt tgatgaatta attccttcat taaaaggaat caaagcaatg
     6721 gtcgtatttg gccaaacaaa agaaaagttg atggatgctg gaaaaaaagc aggcatcgag
     6781 acaatagtaa cagccgattc tgtagaacaa gcagttcctt tagcattaga aaacagtaca
     6841 gatggtgatg tagttttatt atcaccagcg aatgccagct gggatcaata cccaaatttt
     6901 gaaactagag gaaacagatt catggaagct gtgaaccgat taaaatagaa aaagtagaag
     6961 cgagcgataa aaaatgaaaa ttttggttac aggcggagga acagggggac atatttatcc
     7021 agctcttgcc tttgtcaatt atgtaaagac tcaagaaccg aatgctgaat tcatgtatgt
     7081 gggagcaaaa cgaggtctag aaaacaaaat cgtccctgat acaggaatgc ctttccatac
     7141 cttggagatt caaggattta aacgaaaaat ttccatgcat aacatcaaga cggtccgatt
     7201 gtttcttaag agtatccgtc aagcaaaaaa aatcctcagt gattttcaac cagatatcgt
     7261 tatcggtact ggaggatatg tttccggagc ggtagtgtac gctgcagcca aaatgggtat
     7321 tccaacagtt atccatgaac aaaacagtgt accaggtgtt accaataaat ttttaagccg
     7381 atatgtggat cggatcgctc tttcatttaa agatgcggct tccttttttc ctgaaaacaa
     7441 agcagttctt gtaggtaatc ctcgagcaca agaagtagca gatactaaaa aatcagatat
     7501 tttgaaaaca tttgatttgg atccagaaaa gaaaacagtc ttgatttttg gcggtagtca
     7561 aggagcactt aaaatcaatc aagcagtatc agttttttta agtgaatata ttccttcttc
     7621 ttaccaagtt ttgtatgctt caggagatag atattatgaa gagatcctaa ataaaataaa
     7681 tcctgaaata caaaatatta gtgtccagcc ttatatcaaa aatatggcag aggtcatggc
     7741 ttccagtgat ctgctggtgg ggcgtgctgg tgcaacttcg atcgcagaat tcacagctct
     7801 ggggttaccg gcagtgttgg ttcccagccc atatgttacg aatgatcatc agactaaaaa
     7861 tgcaatgagt cttgttcacg caggagcagc aaaaatgatt gcagataatg aactaacagg
     7921 tgaatcacta agtcagacag tcaacgaaat catgggtgat gaagaattgc aaaagcaaat
     7981 gtgccgagct tctaaagaac aaggaatccc tgatgcttca aaacgtcttt atgacttagt
     8041 aaaacaaata atcaaaaaat aaaggcggtg gtcttagtga gtaagaaaaa agaaccaaaa
     8101 aaattgtctc atccgtcttc tgatgaaaac aatttgactc cttggcaaaa ggctaaccga
     8161 aaatatctgg cagaacaaga gatgaaaaac caagagccaa aaaaagaaga cataacatta
     8221 gatacacccc tttcagattc tgaagaagaa aaaacggttc aagaggaaca gcaagaagaa
     8281 agccagtccg agaaaaaaat cgaatttttt gaagagatca agcaaaagaa aaacggtgga
     8341 ccatataacg gttctttttt gaaccggctg cccaatttaa aaagtcaacg gcacaaagta
     8401 ctatttcgtc ggttagcttt gatcatcact gtattgggta taccgctgat ttttttgatc
     8461 tattatgttt ctccttatag caaattacaa gcagttgcag tatcaggcaa taagacagtc
     8521 aacagccaag aaatcatctc tgatacaaaa ttgtcattag gagaaaatgt ttggggacag
     8581 tattttcatc gatctactta tatcgatcgc cttaaaaaag cacagccaag aatcgaaaca
     8641 gcaaatatac atttcaaagg gatgaatgaa ttcgatttgg atgtaacaga atataaagag
     8701 attgctttga tcgcaaaaaa caatcaatac tacccagtga ttgaaaatgg aacagtatta
     8761 gatgaaaaag tagcaaatcc tacaaaaaat ctccctatct tggaagagtt caaagactct
     8821 gcaaaaatca aagaattaac aaagcaatac aatcagttat catcagaatt acaaaaagca
     8881 atttctgaaa tcaagtacac gccaagagca agcaataaaa atctgatcca attgaatatg
     8941 aatgacggca atcaagttat tgtaaatatc aataatcttg cgaaccaaat gaaatattat
     9001 tcgcaagtag ccaaagatat ggatgaaaaa ggtgtgatcg acatggaagt tggtatcttc
     9061 tcgcatcctt acggtagcac tcaagaaacg gaagaatcgg gccaatcgga agaaacaact
     9121 gaaagtgaag aaaatgcgga tcaaacaaac gaatcaagtg aagaggcaga aacgcaaaaa
     9181 gcacaaacac ctggatcgtc tagcacaaac ggatcatctg attcaaatga ttcaagtgaa
     9241 acaagtcaaa tagaagataa tccaagtaca actccgtcaa gtttaaatga aaattgataa
     9301 aacaacttta aaaagtaaga atgtactttc taaaaaccag taatttatgg tagaattgga
     9361 agcagtgaat atatctatta ataagtatat tttaaaacgg acgctagttg ttcgcttgta
     9421 tatggaatag taagtaggag ggaatcccat tcatggcaaa aacaggaatg tatgtaggcc
     9481 ttgatattgg aacgacatct gttaaagttg tcgtggctga atatatcgac agccaaatga
     9541 atattattgg agtaggaaac gcaaaatcag aagggattaa ccgaggcatt atcgttgata
     9601 tcgataaaac ggtccaagcg atacaacgag cagtacgaca agcagaagaa aaagcaggta
     9661 tccagatcaa aggcgtaagt gtcggtttgc cagcaaacat gcttgaagta gaaaattgcc
     9721 agggaatgat tgcagttaac ggagattcaa aggaaatcac cgatgaagat gtcagaaatg
     9781 tggcttcagc tgcccttgtt cgttcaatac ctcctgaacg tcaaatcgtg tcaatattgc
     9841 cacaagattt tactgtcgat gggttcgagg gaatcaaaga tcctcgtgga atgatcggcg
     9901 taagattaga aatgtacggt ctgttgttta caggacctaa aacgatcatc cataacatac
     9961 gtaaatgcgt ggaaaatgcc ggattaatcg tcaacgaaat ggtgattact cctttagcgc
    10021 tgactgaatc aattctttct gacggagaaa aagattttgg tacgatcgtc attgatatgg
    10081 gtggcggcca aacaacaacg gctgttatgc atgataagca attgaagttt accaatcttg
    10141 accaagaagg tggcgaattt gtcactaaag atatctctat tgtattaaat acgtcattta
    10201 acaatgcaga agcgttgaaa ataaactacg gggatgcata cccagaacgg acatcaccag
    10261 atgaagaatt tccagtagat gtgattggtc agtcagaacc agtgaaagtc gatgaacgct
    10321 acttgtcaga aattatttca gctcgaatgg aacagatttt caacaaagca aaagatgcat
    10381 tggaccaaat cgaagcatta gagttgccag gtggcgtcgt attgacaggc ggagctgcta
    10441 gtcttcctgg tgttgtggac ttggcacaag aaatcttcgg cgtaaatgta aaattgtatg
    10501 ttccaaatca catggggctg agaaacccgg tatttacaaa tgtaataagt atcgtggatt
    10561 attcagcaaa cttgagtgaa gtgtatcaat tggcaaaaat tgctgttacg ggtgaaacct
    10621 cagcagctcg tcaaatggta gttgaacaag aaacaacaaa cacatatgag agctatgaag
    10681 cacctgaaga aacaatctac gatgagccgg aaccaaaaga gtccggagaa aatgtaaaaa
    10741 ataaaatcaa gggtttcttt acaaatattt ttgattaatt catcctggga ggaataagat
    10801 atggaattct caattgataa taatatcaat gacggcgcag taatcaaagt catcggagtc
    10861 ggtggtggcg gtggaaatgc tgtaaaccga atgattgaag aaaacgttaa aggtgttgaa
    10921 ttcattacag ccaacacaga cgtgcaagca ttaaaaaatt caaaagctga aacagttatc
    10981 caattaggcc ccaaatatac tcgtggttta ggtgctggtt ctcagccaga agttggacaa
    11041 aaagcggcag aagaaagtga acaatcctta cgcgaagctt tagacggtgc agatatgatt
    11101 ttcatcactg caggaatggg tggaggaacc ggtacaggtg ctgcaccaat cgttgcaggt
    11161 atcgcgaaag aattgggcgc attgactgtt ggtgtcgtga cacgtccatt cacttttgaa
    11221 ggaccgaaac gtggccgttt tgctgcagaa ggtatcgctc gtttgaaaga aaatgtggat
    11281 acattattaa ttatctcaaa taaccgttta ttagaagttg tagataaaaa gacaccgatg
    11341 cttgaagctt tccgtgaagc agataatgtt ttacgacaag gtgtacaagg tatctctgat
    11401 ttgatcacag cgccaggtta cgttaacctt gactttgctg acgtgaaaac agtgatggaa
    11461 aaccaaggta cagcattgat ggggatcggt gtggctagtg gagaagatcg cgtagtagaa
    11521 gcaacgaaaa aagcaatctc ctctccattg cttgaaacat ctattgatgg agctgaacaa
    11581 gtacttctta acatcacagg cggtttggat atgacattgt tcgaagcaca agatgcttca
    11641 gatatcgtag caaatgcagc aactggcgat gtaaatatca ttctaggtac atctatcaat
    11701 gaagaaatgg gcgatgaaat ccgtgtgacg gtcatcgcaa caggtatcga tgaatcaaaa
    11761 aaggaacgca agtctagccg accagctaga caagcacaaa tgcaatcacc tgctcaaaag
    11821 actgtattag atatggatca agctaaacca acttcttcag aagaagaaaa cagttttggc
    11881 gattgggata tcagacgtga acaaaatgtt cgtcctagag ttgatgattc caattttgac
    11941 aatatcgaaa aaaaagaatt tgatacattc aaccgcgaag aaacaaaatc tcatggcgat
    12001 gatgaattaa atacgccacc gtttttccgt cgtaaaaaat aaaggagagg aagagcacat
    12061 gattgctgac aacttgcaga aaatcaagca agaaattcag gattcgtgtg ctcttgttca
    12121 tcgtgatccg aaagaagtta cattagtagc tgtaacaaaa tctgttgaat ccgctcaagc
    12181 tgaagaattg gttagagctg gcgttatgaa ttgtgcagaa aaccgagtag ataaattatt
    12241 agaaaagaaa caagatttat ttgattttcc tgaaataaag tggcatttaa ttggtaattt
    12301 acagcgcaga aaggtaaaat tgattgtaaa tgaaattgat tactttcatg cgttggacag
    12361 tttaagatta gcagaagaaa tccagaagcg gttagagaaa gaattatcct gttttattga
    12421 agtaaatgtt tctggtgagg aatcaaaaca tggaatttca ccagatgagc ttttaccgtt
    12481 tgtggaatct ttagctgcct gcacaaaaat caaggttgtt gggttgatga ctatggcgcc
    12541 taaagaagct tctgatgagc aaatccataa agtgtttggt acgctgaaag aattgcgtga
    12601 tcttgttcaa gcaaaaggtt atgcccatgc accatgtagt gaattaagta tggggatgag
    12661 tcaagacttt caaattgcga ttgaagaagg tgcaacattt atccgtgtag gaacagcgct
    12721 attcaaagac gaataaagga ggaacaaaaa tgccgctttt aaacagagga gcaattgcta
    12781 attttttcgg attagctgat gaggaagagt acgaatacca agattatcct gaaacaacaa
    12841 aaaaggctgt acagccaact aaaaaaaata caacacagaa tcaatcaact cattctgtat
    12901 ctgctgcgaa tactcatgaa aaacaaaaaa cggcgacttc acgtcctgta acgcatgaaa
    12961 gtattcagcc ggctgaaaag aaaaaagtaa ttgaactaca tcaatcacct acaaaaactg
    13021 tagcgaaaga aagaacagct tcaagaacct ctacacaacc taaagcaaat cttgctggaa
    13081 aaatcactgt aatggaacca agagcttatt ctgaagctat ggcgattgct aaacgaatca
    13141 ttgctggaga agcaacctta gtgaattttc atttagtaga agaaaaacaa gcgagaagaa
    13201 tcgttgattt tctaacagga acagtttatg cactagatgg agatatcaaa cgtgttggag
    13261 atgagatttt tctttgtaca ccgactggga tggagattga cagttcaacc gctcaatctt
    13321 ttgcagatgc gaatttgttt gatctgtaag aggaggaact acgatttata ctattgcact
    13381 attacttcat ttacttcaac gggcagccta tttctatagc attatcttga tggtatatgc
    13441 attgctctct tggtttccag gcgggtatca atcagggcta ggacgattat tagctaagat
    13501 ctgcgaaccg tatatcagtt tgttcgatcg tctgcctttg aggattggac ccttggattt
    13561 cacaatttta ggggcaatcc tgatcttgaa cttagccgta caaggtttag cacagatcgt
    13621 tatatggata actgtccatt tagtttattg attgaagcaa caaaatcttt attctcagaa
    13681 tagataggag gggaaaattt tggatgcaaa tgtataccag cactttcggg cagatgaacg
    13741 tacatttatc gattcggtag gcgattggat tgaacaggtt caaggacaat atgcacccta
    13801 tctgaccgaa tttcttgacc caagacaatc ctatatttta gaaacactga ttcgacaaag
    13861 cagtgatttg cgtttcatgt tttatggcgg atatgaacag gcagaaagaa aaagatgttt
    13921 gattttcccc gattattacg aacctgtaga ggaagatttt gaaacagcat tgtatgaaat
    13981 ccattatcct agcaaatttg ccagcctaag tcatgggaaa atccttggaa ctttagtggg
    14041 aacggggatc aaacgctctt actttggcga tatcatctcg gacggtgatc gctggcaagt
    14101 atttattgca aatgaggtgg atcattttgt tgctgcacaa gtaacaaaga ttggaaaggt
    14161 tgctgtacgc ttggaaccaa tcaaatacac agaaatgatt atgccgaaag atagctggac
    14221 acaagaaaga ggaacagtta gttccatgcg actagacagt gtgatctctg agatttataa
    14281 tatttccaga cagcggtcca agcagctgat cgaagctggt aaagtaaaag tgaactggac
    14341 agaaaataca agacctgatt ttgtacttga actattagat attatttcag tcagaggtta
    14401 tggcagagtc cagatccaag aacttgaggg gaagacgaaa aaagaaaagt acagggtact
    14461 gttcggtgta ttacgtaaat aaaataaaaa ggtggtttac tcatggcttt aactccatta
    14521 gatatccaaa ataagacatt cccgacaaaa atgcggggat ataatcaaga cgaagtagat
    14581 gatttcttag atttagttgt tcgtgactat gaggaattga ctcaaagaaa tcgagaatta
    14641 gaaaaagcag tcaaacattc tgaagaaaaa cttgagtact tcaatgaatt aaaagatgct
    14701 ttgaaccaat caatcatcgt agctcaagat acggctgata aagtaaaaac aagtgcaagc
    14761 aaggaatccg aagtgatcgt tacttctgca caaaacaaag cagatgagtt agttgcgaac
    14821 gctgaaaaac gtgcacatca attaacaact gatgcagaag aaaaagcaag aaaaatcttg
    14881 acagatgcaa cagaaaaagc aagacagctt gcgacagaaa ctgaagatct taagaaaaaa
    14941 acacgagtat tccatcaacg tatcagcttg atgcttgagt ctcaattaga acaagtgaaa
    15001 agtccggaat gggatgaaat tttacaacca ttctccagct acgtgactga cagccatgaa
    15061 gtgatcaaag aagttttagc aaaacagctt gacaatgaaa atgatactga tgtaaactca
    15121 gacacaggag tcatagaagc acctatgacg gcatttgata cacaggctat tgatatctcg
    15181 atcgtgcctg gtgaaaatga aaacgagtag aacagaaaag cactttgtta ttcttaaagc
    15241 gagccgatga gagtggaagt tcggtaagac actgcatggt gaaagatcct ctacttgaga
    15301 cctgcttttc cgaaattaga gtaagaagca gcggctgacc tcgttattgg tcacagagag
    15361 tttgcactag atagatgcaa ctaaatttgg gtggtaccac ggcatgttcg tcccttgcgg
    15421 ctgaacatgc ttttttgtgt ccggaaaaca aataaaggag tgtatggttg aatgaaaatg
    15481 aaagaaacct tgcaattagg aaaaacaaaa ttccctatgc gtggtaattt gccaaaccga
    15541 gaaggcgagt ggcaaaaaga atgggaagaa aatcgaatct accaaaaaag acaagagttg
    15601 aacgaaggaa aacctagctt tgttcttcat gatggacctc catatgccaa tggtaatatc
    15661 catttaggac attcattgaa taaaatcagt aaagacatta tcattcgttc aaaatcaatg
    15721 tctggctatc gtgcaccata tgttccagga tgggatacac atggattgcc gattgaacaa
    15781 gtattggcta ataaaggcgt gaaacgtaaa gaaatgtcca tggcagaata cttggaaaaa
    15841 tgtcgtgaat atgcactttc ccaagtagat aagcaacgag cagatttcaa acgcctagga
    15901 gttgcaggag attgggaaca tccttatgtt acattagatc cttcttatga agctgcacaa
    15961 gtacgcgtat ttggtaaaat ggctgaaaaa ggctatattt acaaaggatt gaaaccaatt
    16021 tactggtctc cgtcaagtga gtcttcttta gcggaagctg aaattgaata taaagatgta
    16081 aaatctccat ctatttttgt agctttcaat gtcaaagacg gcaaagatat tcttgatgaa
    16141 gatacagctt ttgtcatttg gacgacaaca ccatggacat tgccagcaaa ccaaggtatt
    16201 gctgtaaatc caaaatttac gtatgtattg gtggaagccg atggccgtaa gtttgtcgta
    16261 gcgaaagatt tgctagaaac agtccaatca gaaatcggtt gggaaaatgt aacagttcta
    16321 aaagaatttg ctggacaaga aatggaatat atgactgcaa gtcatccatt ctatgaacgt
    16381 gaatcacttg tgattttagg cgatcatgtt actttagacg ctggtactgg attagtccat
    16441 actgctccag gacatgggga agatgactat atcgctggta gcaaatataa actatctgta
    16501 gtgtcacctg tagactcaaa aggattgttt acagaagaag cacctggatt tgaaggtatc
    16561 ttttatgaca aagcaaatcc tatgatcact gatctattaa aagaaaaagg agctttgctg
    16621 aagctagatt tctttactca tagttatcct cacgattggc ggacgaaaaa gcctgtgatc
    16681 tatcgggcaa ctccacaatg gtttgcatct atcgataaat tccgtcagaa tattttggat
    16741 gaagtagaaa aagtagagtg ggttatccca tggggtaaaa cacgtttgta taatatgatc
    16801 cgtgaccgcg gcgattgggt aatttcccgt cagcgtgcat ggggagtacc acttccgatt
    16861 ttctatgctg aaaatggaga agcaatcatc acaccagaaa caatcgaaca tgtggcaaaa
    16921 ttatttgctg aacatggatc aattatctgg tttgaaaaag aagcgaaaga acttcttcct
    16981 gaaggattta cccacccagg ttctcctaat ggcgaattta caaaagaaaa agatatcatg
    17041 gatgtctggt ttgattctgg ttcttctcat gaagctgttt tacgtcagcg accagaatta
    17101 tcattcccag cagatatgta tttagaagga tcagaccaat atcgtggctg gttcaactca
    17161 agtatcacga ctagtgtggc tatcaatggt gtagcaccat acaaatcagt tatttctcaa
    17221 ggatttgctt tagatggtga aggccgtaag atgagtaaat ctttaggaaa catcattgca
    17281 ccagaaaaag tgatcaaaca atttggtgca gatatccttc gcttatgggt atctagtgta
    17341 gattatgaag cagatgttcg tgtttctatg gatatcttaa gtcaagtggc agaagtatat
    17401 cgtaaaatca gaaatacgat gcgtttctta ttagcgaata cagaagattt cgatcctaaa
    17461 aaagatactg tttcgtacaa tgatttacgt tctgtagaca aatatatgac cgttcgattg
    17521 aaccaagtaa tcaaagaaat ccgagaagaa gggtacgaca aatataactt catgcatatt
    17581 taccgtacag ttatgaactt cttgacggtt gatctatcgt cattctacct ggattttgct
    17641 aaagatgtag tttatattga agcagaagat gattatcaac gtcgttgcat gcaaactgtt
    17701 ttctatcaaa cagctgtagc tttgacaaaa ttattgactc ctatcattcc gcatacagca
    17761 gaagaaatct ggtcattcct gaaagaagaa gaggagtacg ttcagttaag cgagtttcct
    17821 ggttatgaag agtttgcaaa ccaagaagaa ttgatggata cctggactgc tttcatggac
    17881 ttccgtgata atgtcttaaa agcattggaa gaagcaagaa acagcaaatt gattggtaaa
    17941 tctttggaag caaaactaac tgtttatcca aaacaacaag ttcgtgaaat gttaaaagct
    18001 ttggatgcag atattgcaca attattgatc gtttcaccag actactttga agttatggca
    18061 gcagatgaac aagtgccaga taacgctatg gtatttgatg atgttgctat tacagtggaa
    18121 aaagcagacg gggaaacttg cgatcgctgt cgccaagttc gtaaagatgt aggagtagat
    18181 gaaaaacttc ctcatctatg cggtcgttgt gcaaaaatcg ttgaagactt ctatcctgaa
    18241 gctgtagctg aaggattcga agaaaagtaa aagggaaaag ggggtgtgac atgttctttg
    18301 tcacaccccc ttttgttatc aatcgtttaa ttttccacgt tcacgatacc agttatctgg
    18361 ttgccagatc cattcgaatc cgtctttttc aagtagttcg aaagctgctt taggtcccat
    18421 tgatccagct ttgtagttgg gaaaatccgg ttgtgtttgg tcccaagctt ttcgaatgac
    18481 atccacgata tgccaagatt gagcgacttc ttcccaatga gagaagttcg ttccatcacc
    18541 attcaacgca tctaatagta atttttcgta agcttctggg ctattttcaa ccatttctgc
    18601 actatttcta aattccaatt tgattggttc cgtttcaaat ccttgcccta cttctttccc
    18661 attcaaggat aaagaaaatc cttcagttgg ctgaatatag atcgttagaa cattaggtgg
    18721 aagtgtcgta tcgtcacatg gttcatcgac agatgttttg aatacattaa caggaacttg
    18781 tttgaatacg atgttgatcc gagtcccttt ttcagtcaaa cgtttgcctg tacgtacata
    18841 gaaaggaaca cctgaccaac ggaagttatc gatagtgaat ttgcctgcga cgaatgtttc
    18901 agtctctgat tgagcatcca ctttgtcttc ttcgcgatag cccttgaatt gtacaccatc
    18961 caattcacct tgagcatatt gcccgcgaac gaagttttct aacacttctt cttcgttata
    19021 aatgcggata gctttcaatg ctttgatctt ttctgtacgg atttcttttt cggaaaatgc
    19081 tactggcggt tccattgcaa gtaatgctac aacctgtaaa atatgatttt gcaccatgtc
    19141 tttaagtgcg ccactatgat cgtagtagcc cccgcgatct tctacaccga ggttttcgcc
    19201 aaaagtaatt tggatattgt cgatatagcg attgttccat tgagcctcaa aaatattatt
    19261 ggcaaaacgg atagctgaga tgttttggat catttctttt cctaaataat gatcaatcct
    19321 gaaaatatct tgttctggga atacttctct gatttgctgg ttcaattcaa aagcagactg
    19381 gtaatcagaa ccgaacggtt tttctatgat caaacgattg aagccatttt ttgtcataat
    19441 attttgtgat ttcaaatgag aaacgatcgt tccaaaaaat tgaggtgcca tagccaaata
    19501 atagacacgg ttaccgttta attgatagtg tccatctaat cggtcagaca attcttttaa
    19561 tgtctgataa tgcgttgaat catttacatc atgggattga tagtaaaaat ggcttgcaaa
    19621 ggtagttgct tcttcatctg aaggattcaa tgcacggatt gtttctttga caacttcacg
    19681 gtagtgctca tcgctccagg gacggcgagc ggttccgatt acagcaaaat gctcgcctaa
    19741 atttcctttt ctgtaaaggc ggaacaaaga cgggtacaat ttacgctggg ctaggtcacc
    19801 tgtaccgccg aatatcgtaa acaatacatt tttttcatta gacatgtgga tacactcctt
    19861 tatattttat cgacaaaaat cgtgctggcc gccttaaagc tgacggggat tttctttcta
    19921 tcattttgga tagtgatcgg tccttcataa gctgcgatgt caacgatctc atattcttcg
    19981 ttgatattca ggtcaatagt gactaagtaa tccaacaatt ctttttcatc aagcactcgt
    20041 gcgatacgca cttttgtacc gacaggatag gactccaatg tttgtcggcg ttcttcatgg
    20101 acaacttcct cttttttagg aatcgatcct ccatgaggac aatgttcagg atgattcaaa
    20161 tagttttcta aatgatctgc aagatgttcc gaagtcacat gttccaatac ttcagcatca
    20221 tcatggactt cattccagct atagtctaaa tgttcaacta aaaaaacttc ccataagcga
    20281 tgtttgcgaa tcagactgct agctttttgc agtccgcttg cagtcaattg tacaccttga
    20341 taaggggaat gttctacaag ctcttctttc acaagtttag aaatcatttc actgacagat
    20401 gcagctgaga catctagact agatacgatt tgtttattgt tgactttcgt tgtatctcca
    20461 cctagttcta aaatgatttt caaataatct tctcgatttg gtgtcatatt cgatcatcct
    20521 ttcatggtaa aaaaccatca ttagtaagtg taacaaaaaa aacgttaatt gactattttt
    20581 ctaaataaaa aacaaaggat ttttagacgt ttttacaaca aaaaacgaaa gatgatagag
    20641 aagactcatt ctttcgttta taaaggatat atatatacat tgggagggat ctatttttca
    20701 gataacaact tatatgaagt gtctgcagat tcgtcataaa gagtttgttc gggataagaa
    20761 aagataagat aaaaaccata ctgatcacgt acgactaaac gatattcatc tatttctacc
    20821 acagatgtgc caagagactg accgttgatc aaaaacgtgt aatcagatgt aatctgtaat
    20881 tgttgttttt gctggctatc actaaagaac catgtacctt gatactttga gagtccttct
    20941 gacttttccg cttgtttttt ctttttcaag taaataaaac ttgagagaac ggtaatgagt
    21001 gaaccaagaa aatagatcat tttttttatc accaaatcac tcctttatga gaaaataatc
    21061 gcata
//
LOCUS       DAYYHX010000033        20870 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000033 DAYYHX010000000
VERSION     DAYYHX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.033"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1834)
                     /locus_tag="ACHA6Y_000874"
     CDS             complement(<1..1834)
                     /locus_tag="ACHA6Y_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8986987.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTVQEAPGKAADWIKNKWTGTKEFFSNLWSSIANSASEIWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPF"
     gene            complement(1851..2138)
                     /locus_tag="ACHA6Y_000875"
     CDS             complement(1851..2138)
                     /locus_tag="ACHA6Y_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986988.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            complement(2192..2560)
                     /locus_tag="ACHA6Y_000876"
     CDS             complement(2192..2560)
                     /locus_tag="ACHA6Y_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HFM8986989.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFVDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQVE"
     gene            complement(2606..3118)
                     /locus_tag="ACHA6Y_000877"
     CDS             complement(2606..3118)
                     /locus_tag="ACHA6Y_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HFM8986990.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            complement(3129..3521)
                     /locus_tag="ACHA6Y_000878"
     CDS             complement(3129..3521)
                     /locus_tag="ACHA6Y_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFM8986991.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQLSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            complement(3521..3859)
                     /locus_tag="ACHA6Y_000879"
     CDS             complement(3521..3859)
                     /locus_tag="ACHA6Y_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8986992.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            complement(3843..4169)
                     /locus_tag="ACHA6Y_000880"
     CDS             complement(3843..4169)
                     /locus_tag="ACHA6Y_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986993.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            complement(4166..4495)
                     /locus_tag="ACHA6Y_000881"
     CDS             complement(4166..4495)
                     /locus_tag="ACHA6Y_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFM8986994.1"
                     /translation="MMIADDIKKLLKGTIDEKLEVIERRTNERMKTLLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFV"
     gene            complement(4507..4770)
                     /locus_tag="ACHA6Y_000882"
     CDS             complement(4507..4770)
                     /locus_tag="ACHA6Y_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8986995.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAAAVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            complement(4788..5711)
                     /locus_tag="ACHA6Y_000883"
     CDS             complement(4788..5711)
                     /locus_tag="ACHA6Y_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8986996.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRST
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            complement(5724..6326)
                     /locus_tag="ACHA6Y_000884"
     CDS             complement(5724..6326)
                     /locus_tag="ACHA6Y_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFM8986997.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELAAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            complement(6453..7379)
                     /locus_tag="ACHA6Y_000885"
     CDS             complement(6453..7379)
                     /locus_tag="ACHA6Y_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFM8986998.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLVRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            complement(7384..8883)
                     /locus_tag="ACHA6Y_000886"
     CDS             complement(7384..8883)
                     /locus_tag="ACHA6Y_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8986999.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKYRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQIGIFDSDGEEVINNKKDETGG"
     gene            complement(8896..10140)
                     /locus_tag="ACHA6Y_000887"
     CDS             complement(8896..10140)
                     /locus_tag="ACHA6Y_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFM8987000.1"
                     /translation="MALVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDVAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRIKRMF"
     gene            complement(10127..10660)
                     /locus_tag="ACHA6Y_000888"
     CDS             complement(10127..10660)
                     /locus_tag="ACHA6Y_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFM8987001.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            complement(10687..10911)
                     /locus_tag="ACHA6Y_000889"
     CDS             complement(10687..10911)
                     /locus_tag="ACHA6Y_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987002.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            complement(10918..11130)
                     /locus_tag="ACHA6Y_000890"
     CDS             complement(10918..11130)
                     /locus_tag="ACHA6Y_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987003.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNDAQAMEFMMRTFSASNQQKE"
     gene            11480..11671
                     /locus_tag="ACHA6Y_000891"
     CDS             11480..11671
                     /locus_tag="ACHA6Y_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFM8987004.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSDNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDVTK"
     gene            complement(11664..12005)
                     /locus_tag="ACHA6Y_000892"
     CDS             complement(11664..12005)
                     /locus_tag="ACHA6Y_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987005.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(12304..12771)
                     /locus_tag="ACHA6Y_000893"
     CDS             complement(12304..12771)
                     /locus_tag="ACHA6Y_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8987006.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPPSTANNTFEAERMMIEREEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            13079..13330
                     /locus_tag="ACHA6Y_000894"
     CDS             13079..13330
                     /locus_tag="ACHA6Y_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987007.1"
                     /translation="MTMWSMLLFWIPVCIGIVAFCYFVKHSRTNKLLMLSFLPIVFFI
                     VQIVKYTYIESQEIFIFYVVGLFISVVFFIMILSYFYKK"
     gene            complement(13363..13650)
                     /locus_tag="ACHA6Y_000895"
     CDS             complement(13363..13650)
                     /locus_tag="ACHA6Y_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987008.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKKRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMKAI"
     gene            complement(13647..13856)
                     /locus_tag="ACHA6Y_000896"
     CDS             complement(13647..13856)
                     /locus_tag="ACHA6Y_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987009.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLFIVDILQSARWFDSLEEAN
                     QVSRRLGGLTQVYELVTVDHEEVER"
     gene            complement(13853..14050)
                     /locus_tag="ACHA6Y_000897"
     CDS             complement(13853..14050)
                     /locus_tag="ACHA6Y_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987010.1"
                     /translation="MKAYTIKFYGNKTYVYILSGLRLDFRGYSKEDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEAE"
     gene            complement(14047..14373)
                     /locus_tag="ACHA6Y_000898"
     CDS             complement(14047..14373)
                     /locus_tag="ACHA6Y_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987011.1"
                     /translation="MNYTQQELTDLCPKHVAEFINNEVLPKYADGLNTAENVTDFMIN
                     DAIDRLIFLEIDCIAYYRLHAEVALIDPYIALSQNRKILVAYIDTKQILEGRDVEIEI
                     DEEEVG"
     gene            complement(14370..14651)
                     /locus_tag="ACHA6Y_000899"
     CDS             complement(14370..14651)
                     /locus_tag="ACHA6Y_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987012.1"
                     /translation="MGDMGEYWKDIKPHLKERRKQHVRKMGNSATKNIETLGFEFTHY
                     PNNHQFAINTHNGIIDYWGTTGTWIERKTKKRGKGIRSLRKYINQEDSK"
     gene            complement(14664..14882)
                     /locus_tag="ACHA6Y_000900"
     CDS             complement(14664..14882)
                     /locus_tag="ACHA6Y_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HFM8987013.1"
                     /translation="MISLTIFWCVIGGLLAMLGAYLIYESAKGKKYTAVGIILFVVGI
                     LVAIGAEINLYLNGTTEELMWFLLWTRR"
     gene            complement(14879..15190)
                     /locus_tag="ACHA6Y_000901"
     CDS             complement(14879..15190)
                     /locus_tag="ACHA6Y_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8987014.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            complement(15367..15681)
                     /locus_tag="ACHA6Y_000902"
     CDS             complement(15367..15681)
                     /locus_tag="ACHA6Y_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFM8987015.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKAKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
     gene            complement(15681..15980)
                     /locus_tag="ACHA6Y_000903"
     CDS             complement(15681..15980)
                     /locus_tag="ACHA6Y_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987016.1"
                     /translation="MGKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(15983..16144)
                     /locus_tag="ACHA6Y_000904"
     CDS             complement(15983..16144)
                     /locus_tag="ACHA6Y_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8987017.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERIKAVMVNETARKAINLHIVNL
                     RREIRRLEE"
     gene            complement(16159..16518)
                     /locus_tag="ACHA6Y_000905"
     CDS             complement(16159..16518)
                     /locus_tag="ACHA6Y_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM8987018.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(16515..17366)
                     /locus_tag="ACHA6Y_000906"
     CDS             complement(16515..17366)
                     /locus_tag="ACHA6Y_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8987019.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(17383..18216)
                     /locus_tag="ACHA6Y_000907"
     CDS             complement(17383..18216)
                     /locus_tag="ACHA6Y_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFM8987020.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            complement(18223..18909)
                     /locus_tag="ACHA6Y_000908"
     CDS             complement(18223..18909)
                     /locus_tag="ACHA6Y_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8987021.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(18915..19661)
                     /locus_tag="ACHA6Y_000909"
     CDS             complement(18915..19661)
                     /locus_tag="ACHA6Y_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8987022.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            complement(19654..19995)
                     /locus_tag="ACHA6Y_000910"
     CDS             complement(19654..19995)
                     /locus_tag="ACHA6Y_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987023.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(20053..20181)
                     /locus_tag="ACHA6Y_000911"
     CDS             complement(20053..20181)
                     /locus_tag="ACHA6Y_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987024.1"
                     /translation="MNRAEALKIGVIIANRWWRHNKPNILSQQHIDKQKAWQQIKK"
     gene            complement(20174..>20870)
                     /locus_tag="ACHA6Y_000912"
     CDS             complement(20174..>20870)
                     /locus_tag="ACHA6Y_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFM8987025.1"
                     /translation="VILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAENWADL
                     FWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKELKDQ
                     LPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMVRNKI
                     RVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMPSTAT
                     MTIIKQMELLEDE"
BASE COUNT         5594 a         4463 c         3209 g         7604 t
ORIGIN      
        1 caaatggacc aatatatttt agtataaaat caaaaccaga tttgatttta cttgtaattg
       61 ttttccatgc agtagatata gtatttttaa acccttccca tttttcacca gcacttgaaa
      121 ctaaatcatc aataactgat atgacacctt cttttaaact attccaaatc tctgaagcag
      181 agtttgcaat acttgaccat aaatttgaaa agaactcttt tgttccagtc catttgtttt
      241 taatccaatc tgccgctttc ccaggggctt cttgaactgt agtccaaaca ttgtctgcgc
      301 cttctttaat ggacttccat aaattgttaa accattctcc tgtagatttc catgcattct
      361 gaatccattc tactgccgag cttacagcag acttgattcc ctcccataag ccaatccaaa
      421 agtttctaaa atcttcactc gtattccaaa gatagatgaa acctacaacg agtagtgcta
      481 ccgcagctat aaccagaaaa accggatttg taagaagacc cattgcttta cttaatccac
      541 taaacaattt tgaactttca cttaaagctt taaaagctac tttcacccta ttgatccata
      601 taattactga accaatcata aaaattaacg gtcctatagc agcgacaatt gctcctatgg
      661 ccactactaa tttttgagtt gattctggag cacttacaaa tttttctact aagccggata
      721 tggcatctgc tacttttttg atggatggtg ctagaatctt ttgaattaca atagctgctg
      781 actcaaaagc tccaaacatt tgctcgatgg aagaattcat attatcttgc atggtccgag
      841 ccatatcgtc agctgcacca tcagaatctt tcagagattt tgttaatttg cccaatgaat
      901 caggtccttt atcaatcaaa gccatcatcc ctgataatga ttcttgccca tatagtgtta
      961 ctaaagcatt ttgttgttgt tcaggcgtca ggccttcaaa agctttttta agtaattcta
     1021 cttgagtttt taaaggtttc attttaccgt cagcatcata aaacgaaaca cctaaattat
     1081 ccattgtatc ttgcatagcc tttgttggcc ttgctaacct agacaatgct cctcgcaacg
     1141 ttgtacctgc ttgagaaccc ttaatgcctg cgtcactcat aataccaata gctgctgcag
     1201 tttcttccaa agaaatcccc attgaattag ctacaggagc aacatacttc aatgcttctc
     1261 ccatgtctcc aacttcagca ttggtatccg cagcagcacg agcaaataca tcagcgacat
     1321 gtcctgcttc acttgcttct aaaccaaatc ctctcaaagc agtagcagta ttttcagaag
     1381 ctagagccac atcccctcca gatacagctg ctaagtctaa aagacccggc attgctttca
     1441 tgatttcttg tgcgctaaat ccagcagaag ctaagttttc cattccagca gctgattctt
     1501 ttgcgctaaa agcagttttt gctcctagat caatcgcttg ctgtttcatc tgttcgaatg
     1561 tgtcgccggt tgctcctgat atagctttta cacgactcat ttgtgcttcg aagtcgccac
     1621 caactttagc agctgctacg cctactccta taagaggagt ggtaatatac tttgtcattg
     1681 cggcaccagt accttgcatc actttaccaa cagcggttgt catactattt gaattctttt
     1741 caaaagtttt aacagcatct tgtgcatctt taaaagtctt tacaaatcca ctatctgtgg
     1801 cttttaataa ggcttcaaca gaaaattgtt ccatgattct cctcctttcc tcaagagtta
     1861 gctttagtta gtaagctttg gaatttttta tcttgttttg aaagttcgga aactcccatg
     1921 attgaatctt cgattttttg ataattgaag aattcttcaa aggatcgata tacaggaact
     1981 gtctttttgc ctactttttt ctccgcttgg acttgctgat ttgcccacgc taattcgtga
     2041 atcaactttt ctttgtcaag ccaagataac tgggctgcag tcatacgaat gttgtattca
     2101 tataacgtca ttctttcgat atctgagata ttggtcattc ccaaatatcg aaaagaattg
     2161 ataagaattt gttcgtatgc cagtgcagaa tctattccac ttgttgtttt tccgcttctt
     2221 tcaatttctg attcaggttt cggaccgcta actttcccgc gttcgactcc gccaattctt
     2281 ttaggacttc atcaaacaat ttttcaatgt ctttaacttc atcgatgtaa tcatccaatt
     2341 catctaatgt aatagattca tcttcagttc tatttgctat ttccagaact cgtgataacg
     2401 tgttaacgtt ataagaacgt agctctggta agacttttgc ggagagtccc attccaaatt
     2461 ccatatttcc atcgacgaaa ggcatcactt tatctaattc acgtacaaat ttagtgccaa
     2521 atttaaacga atattcttta cctttaattt ttaatttcaa tgtttttcat cctcctaaaa
     2581 taaaaaagag agcatctaag ccctcttatg ctcctgtcga agttgctttc acggtatctt
     2641 tgaatgcata ttgaacaaca tcggcttgat cttctgtcaa ggttgcataa ccatcttgac
     2701 caacaccatt tactgcaaat gataaactta attcaacgtt atcctctgca gcagccgatg
     2761 gagtaaattc agacacatat gcttggtaat aagtagcttt gtacttattt gcattatcat
     2821 ctgttccctg ttctgcttta ttaatttccc aaatttcaat gatatcccca tttaataaag
     2881 cttgtttcat ttcatctaca tgagaatctc ctttagcaac tattgaagta gccgaaaaat
     2941 catattcaac cgggcttaaa ctttgaacgt ttccatcttt tgtcactgta gagtctgaat
     3001 ctcttgataa accattttcg tgttctgttt gaaatgccat tttccaagcg gcttcctgag
     3061 tttctttttt caataagcga taaagcaaaa tgacatcaat accttttaat gcttccatgt
     3121 tcttcctcct atctaattct aaattcaagt gtgacaaccg cccgttttag cggtgtattc
     3181 gttgttgtgt cgtccatcac ttgaattcca cttgcttgat aatttaaagc ccaataatag
     3241 ccttctgtgg cttctatcaa tctagcttca ttaaaaagag cagatgccat atctgacaac
     3301 tgctttcgtt tcttctgtaa tccccaaacg gataaaacca caatcacaga ccctttaatg
     3361 tcagttttat ttacttcatg gattgtctga gtgttctcaa attccacaaa aggataacca
     3421 acatcctcta aagttttgta atcgtatgtt ttatatccaa gtttgttttg ggatatttta
     3481 aaaagttcat caaaaattga ttgatctctt gtcttaatca tcatttcacc aaggctttca
     3541 tttcagccat aaatttgact ttttggtaat taaacgctgg tctaacataa ggctgtgccg
     3601 acataaatcg agtgccatat tctacataag gtgcataatc tgctgtcggt cctacaatac
     3661 cagttaaacc agcttctaaa agattcatgt ttattgatct tcgtaagtaa cctgtatcca
     3721 ctggcgcacc tttttgcatt cgttcagtca tttcagcagt attacttttc acgacttttt
     3781 gaacgtcatt aagcgttgct gcttttttca gatgtcgcat cagctgatcg attcctttat
     3841 attcaagttg tgccttcatc aagaaccacc tcttgcacaa ttaaactatt tctatatgct
     3901 ggatttctag ctgttttttg ttgccaagtc tttccttcaa tctcgatata gtcaaatgta
     3961 gggatagaaa aaagaggctg cgtcctaatg accttcgccc cttcttccac actaccaaaa
     4021 atagtcacac ttctatcagt gccaatatct gtcacgtttg cctctgttct tgttctttct
     4081 ggtttgcctt caacccactc accgagatct ggatcatatt tagagtcaga tgaacgttta
     4141 acaaatataa tttcatctgt aaatctcata caaatttaaa cctccctcgc tttggcttgt
     4201 acaactcttc ctgatcttta cgcttaaatt catcaatctc attttgatac tctgaaaaat
     4261 ccgagtcagg aaaagccata gataaacctt cttgagaata tgactgcata ccttcttgac
     4321 caattctatt gaatcttttc aacgatactt catatacaac tgtttcaaat tctttaggaa
     4381 cttcttgcgt atttaacaag gttttcatac gctcattcgt tcttcgctca ataacttcaa
     4441 gcttttcatc tattgttcct ttaagaagtt ttttaatatc atctgcaatc atcatatttt
     4501 tacttcctaa ctagcaggtt gacctgtcac attgattgaa gtagtgaatt ctccagaagt
     4561 aaatgtgaat gttgctgacc cttctgctgc aatcgttcca tcaaaaccac cattttcatt
     4621 tttggtcact gttgcgatag ctccatcact tgaagttgct gtagtagctg caacaacagc
     4681 agctgcatcg ctagcatctg caggcacagc tgaaatagta aatgttttag tatcgcctac
     4741 tttaccggtc catgtctttt gatttggcac aataccggta gcaggcgtta cgcttttggg
     4801 gaaatcttcc caaatgcttc atcttttaca atcataaatc caacatccat tgttgcacgc
     4861 aaagcaatca gttcttgctc aaacaagtta actggggttc catcttcatt agttaaagta
     4921 gacaattggg cttcttcaga aatcttaaat gaaatattat atgggatccc ataaaacatg
     4981 taattaaagt ctccagcgta aagtgttcct ttatctaaag acttaaggtc tactactggt
     5041 aatccgtcaa ttgtattagt agagcgatca taaataaact caacatttga cccgactgtt
     5101 tgagctgcag aacgtaattc tgtacgattt tttcggtttg aaataaacgc attaggttcg
     5161 aattcatttt ctgctaactt gtcttctaag gctaggatat tatcataagt caatccgcct
     5221 tcaaccacat tccccgcact aataactgat ccgtctagtg actgaggaaa tgggttttct
     5281 ttatttaata aggcagctgc atcaaatttt ttatagaaag cttcagcaat ttttggctgc
     5341 atctcctcaa agaaatctga taatttataa tttaaatatt cacgagaaac cggaagaatg
     5401 acaccgagtt tttttgcagt catcgtagct tgcatccatt taggtttaga cgttttaatt
     5461 ttttcacctt cacccaccca gtatgcgcct ggtccttctg caaagtattc aaatttcttt
     5521 tctttgtcag tcatttcttc gtattttgct aactgcatga tcttagaatt ttccataact
     5581 tcactcaaaa tgagcgtatt atatttatca ggaatttttc cctctttcgt ttcatatacc
     5641 aagacattat ctggatccca tgtttgagca aacatttgca agttcattgg taaaagttgt
     5701 ttctttttca ttaagttttc ctcctatttg ataattcgat ttttagcagc tagtttagcc
     5761 actgtttctt tagtattttt cgatgctgta aattgtccac cttcatttgg tggtgtttgt
     5821 cttgcgtttt ctctcttaat caaagaagca aagttagtga tgactgctac agcttgtttt
     5881 gtggcatctg catcatcaga aacaatcaga ccaagtaaat catcatcgtg tggtaaattt
     5941 gcatctgtca gcattttaga agcttctttc gtcatttcag atagtgcctg tccacgcttt
     6001 aattcagcga tttcagcttc tttttgctcc aactcatgct gtagtttttc ttccgcattc
     6061 atttttgcca gctttttagc ttcttctttt tttgctgcta gttctttttc ccacgctgct
     6121 tttgctttat tcgtctcagc agcgatcatt ttcgctactt catcacgaga aaatgttttg
     6181 ccagtattgt tttcttcttt tgcttcgggc ggtgtctctt gtgagccagc tggtaggttt
     6241 ccttgttgtc cctcatcacc agatccacca tctcctggtt cagaaaaaaa ttgtaagttc
     6301 attggcataa ataaacgttt tttcatgatt aatcctccac ggttacgccg ctacccgata
     6361 aatttgacca gttacgccgg tcagccgaaa atagctttct ctttaacgcc tgtaagctgt
     6421 aagaaggcac aataaaaagc cgttaatttg tgttaacggc ttgatattcg ttctatataa
     6481 ggtgctgtac tgcatcgaca aaatggatgc atattaggag cgttacttcc tggctgcata
     6541 tcggcaacat caaaaacttg attatttaac ggtatacata gtttgcacgc cgttggttct
     6601 gctatataga tgtactgggt aatgcctgca tctctataac ttcgttcttg aatccctacc
     6661 tgaactctag tcgtttcagt caccatcaaa cgttgagtgt tgaacttagt gttttctcgt
     6721 ccttcagctg ttaaatattc tgccaattta gatgcaagtt gcttgggatt tttccccatc
     6781 gttatacttc tgactaacaa cctatctaat tctgatttca attcagattg atacatccat
     6841 aagcgatcac taaaagacac gtcatcactc aaaaaggagc tgtttattac tagttctatc
     6901 agctttgcat atccacttga agcaatagtc atttctaaaa taccggcttg tctcttcaat
     6961 tcagctaaac cagcttttgt taattcattc gaaaagtact tatccaattc attaaacagt
     7021 gaaatcaatt caagcccgat attagctttt aagagctcta atctatttac acgcatcgta
     7081 agattgtata gctttaattc ttggtttgct gtaggagaaa aatctttctc tttgacatac
     7141 ttctttgctt tgcgagcgaa tgctttgaca tccatttcac tagcacgctt catcgcttcg
     7201 ctacgagtga ttttctggcc attggagaaa ctgtcccact gcgcgtctat ttctttttgt
     7261 atcgcatctt gtgcgtattg catgcgactt ttaatttctg ccatgcgctt tttatcatct
     7321 ttaatttgtt gcttttgcca ttccttttcc cgtttgatcc aatattcttg ggagttcatt
     7381 taatcacccc cctgtttcat cttttttgtt gttaattact tcttcaccat ctgaatcaaa
     7441 aatgccaatc tgcttttgcg tttctttatt tactcgtttc aactctgcct gtacatctgg
     7501 aacaaaagaa gcgagtccta agatcgtctc ttgactgagt tcagctccag catcaaccaa
     7561 agatttcaac tcttccagaa tggctttagg tagattaggc gtaaatatta cacgtaagcc
     7621 tttcaaatcg gagttatcca tttcagaaat acttgatttt aggctaaata aaagacgata
     7681 acgccgcata aggccttttt tgaatagcct ttgctttgtt gccgtcattt gttcaaatcc
     7741 aaataattta tatttcattg cttctcccga ttgcactccg gaaaaattgt catcagtaag
     7801 atcaggaacc atggatattt cgtgaatatc cttgcgtact ctgtatttgt atgcttctac
     7861 accgttcaca tcatattgtt tgtaaatata tcctgcagtc acacttgttt tattaccgtt
     7921 cacatcagtt ccagactcaa gcaaaagcat attcgcttct ttctgcttga tggcgtcctc
     7981 tgtggatagt cctgctgctt caatatcacc actaataact agaagagcat cgtttagatc
     8041 agtcatataa ttggcggtat cagactgccc tgcatcgtat agatcaatca aagatagtac
     8101 atcttcatac aagcccatcc gaaaacgatt aggagaatac tctgtaatag gtacctcttt
     8161 atattcatgc ggttcatcct ggggattctt taactcaatt gctgttagtg tcgtctcatc
     8221 ataagtgata cttttttctt ttgtgtatac gattggttga atgtactgtt tatcagcatc
     8281 cttgttgaat cttgtcttag gataccgtac agccaaaata ggctctcgtt ttactgtagt
     8341 atcatataca acaaacgttt caaatacatt agccaaatca acataatctg tatcatctga
     8401 atctcgatag ataatctcat aggctctccc atacttatcc atatcaagcc agagttcagc
     8461 atttaaccca tctatgtcat tattagtatt aaactcttcg atttcttttt gttgatttgt
     8521 gtcctcgatt tgcactttta taggattgcc tgtgttgtac ccaacatcaa acgtacaaag
     8581 aacttttcca aagttatgtg ctgatcggtg atccgctttt tctttttctc tgcgcctacg
     8641 gttatccatg atatttgtat ttctagcttt ataataatca tctaatacac ttagcctttt
     8701 tacctgatat tcatgatgat gttttatcat tgctgctaaa gtatctaaat cgttaagtaa
     8761 atcttctgct gagctaaatc tatagtgaag atttgagtct acgctaaact ttacataatt
     8821 tgtgttcacg tcattagaat aatgtatatc agctccgtat tcaaactcgt ttaccttatc
     8881 catttttcac actccttaaa acatgcgttt aatacgttta atcttttcgt tgacattaac
     8941 ttttggtttc ttaagaagct catgagtata tatcgcatat cgaacggagt ctagcacatc
     9001 atcatattct tttattggct cgcctttttt cttgtcccaa atgtattgat aaatttcatc
     9061 ccgaaatttc atcactctat cttgacagat aaacaattta tcttgtttaa atctcttggc
     9121 gacttcctca actccagata accgtgcttt atgagcattt ttagcattaa tcttttctct
     9181 tacaaaccta gccacatgct ccggtctcgc agagtcacaa taaaaaggta ctcgtaagcc
     9241 gtaacgctct tgaatgcctt ttgccacatc tacccaataa tcaatctctt caaattgagt
     9301 agcatgttct tcgattaaat aagctgttcc gtcatccgtt tcacctataa ctacgattga
     9361 accccagtgt tcatatcccc agtcaacacc acaataaaag ttgctcaaag gtggtaagtc
     9421 ttttgactgg atgtagtgct tgctagcatc aaaatcacga taaacgacac cttctgcaga
     9481 aacccataaa cctttgatat cacgatcata aaacatgccg cttggtgttg attctttaat
     9541 attgttacga tatcgttcag ataaaaatgt attatcatct aattcaaagt ggaacgattg
     9601 aatatttttg cttgaattgt cgatatactc tttctttagc cagtgttccg ggttgtcagg
     9661 gtttgtatca gctagtattc tcgctcctgt tcctgaacaa cgtgaaacga tttcagcaaa
     9721 tacttcttgt ctagctagcg atgcctcatt gatataagcg ccatatgctg tcataccacg
     9781 aattgcgcca acaccgccaa tgtttcctgt ataagcttgt acgaccttaa cgccaaatag
     9841 cttaaagttt ccatgtttat cgaatttagg ttctatgctg tacatgttgt atagttcctg
     9901 taagatgttc ttttggattg ttgcacttga aactcctgct aggatataca ttggttcctt
     9961 aatgtcttct tcatcggcaa tctttcgcac acgtcgcaat tcaaacaaga acaaatcatt
    10021 gtttattttt gtttttccag aacgttttgc tccatgtaat aaagtaataa accaatcatt
    10081 cttaatggtt ttatttaaca cgtcgatttg ctttgggtta taaatatcaa ctagtgccat
    10141 ctaattcacc actaatcttt tctaacaact catcaagttt ttcttcggta gaacgctctg
    10201 agtttatctg taattgttct aattttgctt gcattaattt tatttctgtt tgagcttttt
    10261 ctaattgcgt ttgtgataac agcgatttat acttcaaata caaatccaag gctttcaatt
    10321 tgctttctaa atctggtgta tattcatatt gcatatgttt aataaccttg tttttctcca
    10381 agcggtctat ttgcttgctc acgcttattt gtacttctcc ttgccaaata tctatcaatt
    10441 catttaaagc gtcctcggca cttaatttgc gcttcttttc gataggattt agccgttcat
    10501 taatatactg tattatgtta ggtttagtta ggttttcctt acctattact cttgctgatc
    10561 gtttgctata acctgcttta atggcggctt gagtagcatt accaccattt tttatatatt
    10621 catccgcaaa agctttttgt tttggtgtca aatttaccac attcaagcca ccacctttca
    10681 attttattaa caatatctct caatatttgt ctgtatgttc tgctcactaa aatatccatg
    10741 gccgcagtaa cgaagattgt acttatcaat ctcttttggc gtagcttctc tggtcatttc
    10801 aatgatggag tacttctttt taatctggac tgactggaca actctaattg gatcatctgc
    10861 gtttggttgc ggatatttat ttgataatga tacataccag tagttcctca ttacgtatca
    10921 ctctttctgt tggttactgg cagaaaaggt tcgcatcata aattccatag cctgtgcgtc
    10981 attaaatcct tgcagaataa gctgatcgta gaaatactta gcttgttttg cgattaacgc
    11041 taagcttttt tgagtttcgt agaatgtcgc ctccattgtt ttgtttgctt cattcgtttt
    11101 aaacaactct cttaattggt tttcgttcat ttttttactc cccttccaaa ataaaaagac
    11161 cactcgatga gtgatctaat atgtaaaaac tacacctcat aaacgagatg cagttatagt
    11221 tctatcaagc aacctacacc gattccatcg attactatcg acctcgcctt gctcgtgtac
    11281 tttgagcgcc catttccaac cctcagttgc caaagtcact ggcaaggaat cgaaccttgc
    11341 atggttgccg aagcattgac ctaacacaca tgcttagcgt ctaccctttc cgccacagtg
    11401 acaaattaat attgtgaaaa taaatactaa gtgtataatt ttatttatca gcgagtggtc
    11461 cgctgaaata aattaaaagg tggtgaaaaa tttgtacttt gttataagag aagcaactaa
    11521 tggccaatat tacttcgtta ttaagtccga taacaatgaa gtagtagcaa ctagcgaaac
    11581 atacttgact aaatattccg ctgaacaaac cattaattct ataaaaaacg gaatcaccaa
    11641 agactctcaa gttatcgatg tgactaaata agacgactaa cttcatttgc tagggagttc
    11701 atttcagctg ctttttctac caaagcagtt gagatacttt ctagcttttc tgatttaaca
    11761 gaattaaata ctggcgtctt gatactaatc gatacatctt gacgaggatc aggactcgtt
    11821 gctctatttt tttgcaccac tgatctgtac ttttctactg aatgatactc aattacttgt
    11881 tctaaatgtt ttttgtactc tttacataca gcttcttttt ggatcaattc taaacgttgt
    11941 tgctcaataa tatcaatcaa ccgttcccga tccatacctt gatactttat ttgctcgact
    12001 tccattcgtt ttccctccaa tacataaatt aataaacagc aacggatgat agataataag
    12061 aacaatttag aaggagttgg aattcacatc cttattctta atatttccgt tgctgtctat
    12121 cgaagcttaa ttgtgaaaca ataataaaac gatgttcctt ttattattat tttgtctcag
    12181 acctatcact aatctttcga cactatcata ataaatcatt taccaggtaa gtgattggta
    12241 taaaaaaggt ataaaatgga aaccaaatgg gtaatcaaag ggtataaaaa gtgtaaaaac
    12301 tggctactta aaagcaacca gttctaacga tgaagcaaat tggatgataa ttctgtttga
    12361 ttctacttta accgattctt cgctagtatt attcctttga gcagttacat aaatgggcag
    12421 accattgata taacgatcat agaatatctt cttgcgcctt tctgttacat caggtttatg
    12481 cggatgctga attgctgaat agccacgaac aaataatttg tgcaagtact caaattcttc
    12541 ttgcgcttcc tctctctcaa tcatcattcg ttctgcttca aatgtattat ttgctgtaga
    12601 aggtggaacc aaggagtaag atgccgttac tttaggctcc cgaggttgac ctatcctaca
    12661 tctagctgac agatatgctg aaagaaaaac accaacatta tgttttgtgc gatccatatc
    12721 cacatctttt gcctctggtg tctcatactt ctttacatcg aaaagtacca tccattgatt
    12781 cctccattta tgatataata attgtgtcag aattattaat taaggtcgga ggaatccggc
    12841 tttttttata cccatgctta tgctaagctt ttcaagtagc gaggttgcac tcgttaccca
    12901 tatacatgtt gagctatctg gcggaaaaca gatggctcac tatttcaata ttctgctaag
    12961 gacagccagt ggtcggctgt cttttttata ttttaatgag aagccttact tattttttta
    13021 tctttattac gaaaaatgat attatctatt gataataaag cgtataggag atggttgagt
    13081 gactatgtgg agcatgctgt tattttggat tcccgtttgt attggtatcg tcgcattttg
    13141 ttactttgtc aaacactcta gaacaaataa gctcctcatg ttatcttttt tacctatagt
    13201 attttttatt gtacaaattg ttaaatatac ctatattgaa tcgcaagaaa tattcatttt
    13261 ttatgtggta ggtttattta tctctgtggt ctttttcata atgatacttt cctattttta
    13321 taaaaaataa atttttctct tagaagtaca ttttttcgct gtttatatag ccttcatatc
    13381 caccaatctc gctacagcta aattctcttt gcttttcgct aaccacttat cacattccat
    13441 cgtgttttca atacgaatga tcgctgagtg attatagacg tgttctacat atccacgaaa
    13501 tggatagatg aacccttctg cttcgcagcg aaccatgtca ccgactttga attttggttt
    13561 cttacgtttt ttaggattct ttgtcggcat atctagcatt aaaccgccga taccatgact
    13621 actagcgtaa aatccgtctt ttaatttcat ctttctacct cctcatgatc gacagtgacc
    13681 agttcatata cttgtgttaa tccaccaagt cgtcgtgaaa cttggttagc ttcttcgagg
    13741 ctatcaaacc atctcgcact ttggagtatg tctacaatga ataatgtgtt agcccctata
    13801 gagcgttcat cacttctata aaatttattc ccgaacttta ctacataaat cttcattctg
    13861 cttcctcctc aatcgtccaa gcatttgtaa atttctctaa gcttattagt tcatcttcat
    13921 ttaaatatcc gtaaccatat aaggattcta aaaggtcttc ttttgaatag cctctaaaat
    13981 caagtctgag accgcttagg atgtatacat aggttttatt tccataaaac ttgattgtgt
    14041 atgctttcat cccacttcct cctcatcgat ctctatctcg acatctctgc cttctaaaat
    14101 ctgtttggta tcaatatagg ctacgagtat ttttcgattt tggcttaaag caatatacgg
    14161 atcaatcaaa gcaacttctg cgtgtagacg ataataagca atacaatcta tttctaaaaa
    14221 tatcaaacga tcaatagcat cattaatcat aaaatctgta acgttttctg ctgtatttaa
    14281 accatctgca tatttaggca ggacttcatt attgatgaac tcagctacat gttttggaca
    14341 taaatcagtc agttcttgtt gtgtatagtt catttgctgt cctcctgatt aatatatttc
    14401 ctcaaactac gtatcccttt accacgtttt tttgttttgc gctcaatcca cgttcctgta
    14461 gttccccagt agtcaataat tccattatgg gtattaattg caaattgatg attgtttgga
    14521 taatgagtaa attcaaatcc caaggtttca atatttttag ttgctgaatt gcccatcttt
    14581 ctgacatgtt gttttctacg ttcttttaga tgcggtttaa tatctttcca gtattcgccc
    14641 atatcgccca tttcgctgtc ctcctatctc cttgtccaca ataaaaacca catcaactct
    14701 tctgtcgtac cgtttaaata aagatttatt tctgcaccaa tcgcaactaa aattccgaca
    14761 acaaacaaaa taatccctac tgctgtatat tttttgcctt ttgcactctc gtaaatgagg
    14821 tatgctccta acatcgctag taagccgcca attacacacc aaaatattgt taggctgatc
    14881 atttgctgtc ctccaaatca ctcgacttca cgaatacacc atctaccatt tttcctgtgc
    14941 gtcctttgat ttcgttgtat gcttggttca gacactcgta caaatccata tcattttgca
    15001 tagctaaaat aatcaaggtt actactacat ctccaattcc gtctcttaaa ccatgttcat
    15061 cttttctagc tagagaagcg gcaacttccc cgatctcttc gatcgttttt aacatttgct
    15121 tgctggaatc agcttgatcc aatcccttat ctttagccca ctgctctact tttgtgatta
    15181 gttcgtccat tattcattct cctttatata tttaagttgg ataatacaac ttgtaacaaa
    15241 tgaagctaat gttaaaattg tgccgattgg tgttaaaaaa ccgcttttta tcgcattaat
    15301 taatatagct acaaatatcg ttatatagaa taaaaagtga attgacgcta acgttgaatt
    15361 aaacatctag tcctcctcga aatactcatt cagtatctct ctatactttt ctacaaattt
    15421 gaaacgatcc tgatgaagtt tcttgctcca atttgtttgc cgatccagct cacgcatctg
    15481 atcgaaccct ttttgaattt cgttgtaata aaattcaatg tttgctgctg ctttccaatg
    15541 cctgctactt cgcactcctg ctcctgtttc agccatttcc aacttaacta attccgctcg
    15601 ttcttttgct tttttatctt tctgaatctt catcatgatt ttcttgagga tgatatcact
    15661 gtattgtgta atgagatcca ttatttctcc tccacatacc taaactgtcg cccctttgaa
    15721 tcaatccata agctcctagc tctatcccag atgatgtttt tgctcagtcc agtaatttca
    15781 gataactgtt cagctgtacc cgttactaga attcgatcac catgccagat tgcaattttt
    15841 ctcggcgttt tccgtttagg cttttcagtc cacattgatt taccgagctt ttggacttct
    15901 gcaactattt ctttgtcttc ttgccaattc tcagaatgtg tcagttcaat gattcgtttc
    15961 attgctgctt tcttacccac gctcattcct ccaatctacg aatttccctt cttaaattca
    16021 ctatgtgcaa attgattgcc tttctcgccg tttcattgac catcactgcc tttatccgtt
    16081 ccagatcgtc aatctcacgt tgaaggcttc gaatacgcat ttgaatcact tcttctgttg
    16141 tcatgatgga ccacctcgtt aaaaacgctc ttccttgaac gtattccgat attttttagc
    16201 taatatcaac ggcacttgat attgatgaca gaacaacttt gccttgatct taaagtcttt
    16261 tgtctgcatt cctttgacat ctacgacttt gacaagtttg ccgtttttat aaaatgtgaa
    16321 gtcgggaata tactcgatct tgcgatactt ttttccatct agttcaaatt tcggcatcag
    16381 ctcaaatctt tcctgaagtt ttactttcca gccgttcgct tctgcttgcc acaaggctaa
    16441 atcgtaatac tctgcttccg cgatagaatc aaacttgata cctcgatgaa cagttttctt
    16501 attacggtat ttattcatgc gatactacct ttcactggtt ttatgcgctt gtctgctgtt
    16561 tgttggaatt tcagcgcata accttctgaa ttcttaaata ttctagaaac aattctttcg
    16621 ccgtaggctt ctcttagttc aggaccggat aagtttgttg tgatgatcgt tgccttgttc
    16681 tgtctggctt ctaagagcgt gtttaacgtg ttgtttgtaa actgcctact atttgatacc
    16741 ccgctaccta attcagctcc aatatcgtca aaaaccacca aatcagttgt tttgatatcg
    16801 gctataagcg atccttcaat ttcttttctc agttcagcat tgttataaga aaactttatt
    16861 tgctctaata actcttgata gcttataaaa agtattttct tgtcataatt tgagcgctca
    16921 agtatttccc aagctgtcgc cattgacaag tggctttttc cgcttcctga tttccctgat
    16981 agaatgaaat gtgcaggatg gttcagtaag acatcattta caaagctttt agctctttct
    17041 aaagcaattt tcgtttcttg gtccactacg tgataattct ccattttgca tttaaacaaa
    17101 gttttatctg ttaataccga accattttga aaaaaactca acgctcgtgc ttttaagctg
    17161 tcgttatata tccgttcggt ctgtatatcc tctttcacac gtaacgcttt ataaccacaa
    17221 ctcatgcatg ttggtttaca acgttctgaa ccatccttat ttttagctcg ccaactatac
    17281 aaaggttcgc tacattctgg acatttcccg ctttgcacta atactcttct tattagtttc
    17341 tccatagcat ttgctaggct ttccatgtga tgcatctcct ttttaaattg gcaagtcgtc
    17401 atattcacta ggattgctgt actgtagttt ttgactttgc tttttatgat tattcttgtc
    17461 tgctttgatt tcgaatttga gcttctcaaa tttttctctc aatttcttag cacttctaat
    17521 atttccaaac caaaattcat ttgtaggtag ccaattgatc acatactcaa tcgcttctat
    17581 agacgcttta tctctttctt ccatcaacct gattgtgtct gcccattttt cgatatctac
    17641 tttgttcatt tcttttggaa aatcttcagt taaattactt tgcaattttt tagcaaggcg
    17701 taagtgttcg tcagaatact tacctttctt ttcttcttta tctatatcta tatctttctc
    17761 tatctctatc tctaactctg gtgtagtttt gtctggacat ttgtccgaca cttgtcctcc
    17821 agttattaaa ttccgttttg cctcttctat tttctttctg tattctcttt ttctatctgc
    17881 ttcagttgag gattttccaa tgaaactttg tatatcagac atataaattg ctccgttatc
    17941 taatacgtca ataagctgca aatcacggaa aatttgtacc gctttttcta cgactcctac
    18001 agaatgtctt gtaatagttg cgagcattgt agagttaaat ggaatcctgt cattaaacat
    18061 caacttacct tcgtgtttta gacttcttaa ataaagtttg agaagaatat tagaataaat
    18121 atagccatct ggcatacttt ctaagagaac catctcgtca ctatcgaaaa aattctcttt
    18181 tagttttaaa tagtagtagc gtttgttgtc agacaatatt ttttaccctc ctattctaag
    18241 tttcttaatt gtttcctggt ttaacttgat ccctttgatt tgatatttat ttttgaaatt
    18301 gatcacacct atattgtgtt tctccgtgtg atgaatcctg cagagtgctg caaatgtgta
    18361 ctctgaatga tcaacttctt tgcgctttcg tcttcctagc gctttgtcaa agtgatcgat
    18421 gtcagctcct gttttgccac agatgcagca gactcttttt gtgatgcatt tgtagaagta
    18481 atattcttga ttcgctggta aaatctcata gccttctttg aaaggaatat gatgttcaaa
    18541 gatgaaatct aagatgatat ttgctaagac attagcatca ctcacagttg tattcgattc
    18601 gtctttgagg cttattttgc gccctgtgac gccttcaaaa cggaagtaaa agaattcctt
    18661 ccagaagtcc gttggcatgc ctgtatcgat gaaaatatcg cctatcagcg catagatgaa
    18721 gtttcgttgc tgcacagtaa atcgacgtgg atcaataaat cgaacttcaa taatccgatc
    18781 gccatcatat ccgtcataca tcgtctttag tcgatcaatg ttcacttctt cattgatcgt
    18841 tgcacctatg tcttttcctt tgaacttttt cagaaccgct gaatatgaat cgatcaatgg
    18901 tttaaacact catatcactt ctcttttgtt tcttctctgt actgatcttc aatccagtta
    18961 acgcctcgtt ttagaatgcc caagtctctc ttggtccatt tactgtcatc agcggttata
    19021 gaagccgcat cagtcaatgc aacaattgct tcatcaattg atttttcgta cttgttagca
    19081 accagttgta aagcatccaa gaatagcttt ttgcttcttt gagtagctgg ttcaagcatc
    19141 gagacatctt ctggcatatc ttcgccagca aatatatata gccctagccc aaacattgct
    19201 agatttttta caagacagcg catgatcgtt ttgttgatat caaacatagt tgctgcttca
    19261 actcgctttt cgatttttcc aacaatctct tttttcttcg tttcgttatt ccactgataa
    19321 tcattgactt cgtaggtata tggctcatct ttcattgcct tgtttgcacc atccatgact
    19381 ggtaaccaca tgtcacgctt tactccgttg actgtgatac tggtaaaaac catatagcct
    19441 gttttcttat caaagacata cggcagatat gtttctggat catgatagat ttcataatct
    19501 actgcctcac acactttgct aacttctgcc caagcccatg cccacgaaag ataagtgagg
    19561 ttatttcgtt tttcagtaac atctttcaca tttattttgt ataaggtatt aaaaagcgta
    19621 ttatcacttc tatttttcaa aggttgttcc ttttcactca tcaaattctg cctccatttc
    19681 agcaatgtat ttcttacctg atccgtaata agagatatca atcaagttat ctctgtcata
    19741 ctcttctagc gcatcaatca agccatcttc gatgacatag atatattcag tttttttgga
    19801 cttcctcgat aaatggataa gatagacatg atcccaaata ctcacaaaat ttcccaaatc
    19861 gtcttgatca catgctagtt cttcatccgt caaaagatta cgtctgattt ttcgattgct
    19921 tgtttccttg atattcgatt tgccccaact aggatcagtc aaatattgat ctagagtgga
    19981 aagttctttt ttcatgtggt aacatctcct tagatgtatt ttctttgtga ctcattgctt
    20041 tggtcggctg agtcactttt ttatttgttg ccatgctttt tgcttatcaa tatgttgttg
    20101 gcttaggatg tttggtttat tgtgtctcca ccagcgatta gcaattatta cgcctatttt
    20161 tagcgcttca gctctattca tcctctaaca actccatttg cttaataatt gtcatcgtcg
    20221 ctgtagaagg catccaattg ttaataaaat cgataacatc atcgaactgt tttgccttga
    20281 tacgatttct agctactgct ccagtgattc gcttaattcc gccattcaag tctttaaaca
    20341 attctgcttt tgctttgcta tttaaatgct gctgattaca aataactcta attttgttgc
    20401 gaaccatatt agaaattgtt cggtaatctg gttcagctag aagttgattg ttcttcaaat
    20461 cgtttaaatc agactcgata gcgtctactc tttcattcgt ttcttcgttt gcagctagag
    20521 caagtaatgc catttctcgt ttagttgctg gaattgatgg caattggtct tttaattctt
    20581 tttccatctt gttgaaggcc tcaatatatt gaagtttaaa acttaaagct ttcttaccag
    20641 tgaagcccat tgctaataga gagaagccgt ctctattcat ataaataatt cggtatgatt
    20701 gtttattttg aggatgaaca taagtatctt cccaaaatag gtctgcccaa ttttcggcaa
    20761 ccccctcttt tagttcatca atagccgtca aaacatgtct gtgcttttta tcgaagcttt
    20821 ctgcaacttg taagctcgta gtcacagctt ctttattttt caaaattact
//
LOCUS       DAYYHX010000034        20588 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000034 DAYYHX010000000
VERSION     DAYYHX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.034"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACHA6Y_000913"
     CDS             352..1041
                     /locus_tag="ACHA6Y_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8987026.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACHA6Y_000914"
     CDS             1028..2236
                     /locus_tag="ACHA6Y_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8987027.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACHA6Y_000915"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACHA6Y_000915"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM8987028.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACHA6Y_000916"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACHA6Y_000916"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM8987029.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACHA6Y_000917"
     CDS             complement(6339..7034)
                     /locus_tag="ACHA6Y_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM8987030.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACHA6Y_000918"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACHA6Y_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM8987031.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACHA6Y_000919"
     CDS             complement(8233..8424)
                     /locus_tag="ACHA6Y_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM8987032.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACHA6Y_000920"
     CDS             complement(8445..9335)
                     /locus_tag="ACHA6Y_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM8987033.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACHA6Y_000921"
     CDS             complement(9322..10089)
                     /locus_tag="ACHA6Y_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8987034.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACHA6Y_000922"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACHA6Y_000922"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM8987035.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACHA6Y_000923"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACHA6Y_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM8987036.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACHA6Y_000924"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACHA6Y_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM8987037.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACHA6Y_000925"
     CDS             14660..15043
                     /locus_tag="ACHA6Y_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987038.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACHA6Y_000926"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACHA6Y_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM8987039.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACHA6Y_000927"
     CDS             complement(15987..16805)
                     /locus_tag="ACHA6Y_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM8987040.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACHA6Y_000928"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACHA6Y_000928"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8987041.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACHA6Y_000929"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACHA6Y_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM8987042.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17934..19268
                     /gene="dnaA"
                     /locus_tag="ACHA6Y_000930"
     CDS             17934..19268
                     /gene="dnaA"
                     /locus_tag="ACHA6Y_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM8987043.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19466..>20588
                     /gene="dnaN"
                     /locus_tag="ACHA6Y_000931"
     CDS             19466..>20588
                     /gene="dnaN"
                     /locus_tag="ACHA6Y_000931"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM8987044.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVR"
BASE COUNT         5878 a         4206 c         3789 g         6715 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgcggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggagtcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa atggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggtgattt cctgtgtatt ccgtctccta actattggta tatctatatt
    17701 ttttatccac agatgcaaga ggaaatctat tgatttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt tttgacaact gtgtttttct cttttttctt tttgtcgatt
    17821 gtgtggataa ttgtaattaa aaaatttttt tcttcggttt ctgtggaaaa ctattgaaaa
    17881 aaatgctaaa ttagtactag ttattcactt tttaatgaag gaggaggcct tatatggtat
    17941 ccctcgatgc tttatggaat gaattaaaag caacatacca aaaggattta tcgcctgcta
    18001 gctataatac atggatcgaa acagcgaacc ctcgaaccct tgatcaaaac cagctcgttg
    18061 ttgaggttcc tagcaagatt cataaagaat attgggaaaa aaacctggcg acaaaaatcg
    18121 ttgaagtcgg ttatatgtta tcaggaaatg agattatccc tcgctttatt actggcgaag
    18181 aagcagagca agaagaagtt atagaagaaa aaaatccaaa agttgtggca cctagtccgc
    18241 taaaaaaagc catgctgaac cctaaatata cctttgatac gtttgtcatt ggtaaaggaa
    18301 accagatggc tcatgcagca gcgctcgttg tagcagaaga tccaggttct atttataacc
    18361 cgctcttctt ttatggaggc gtgggacttg gtaagactca cttgatgcat gcaattggtc
    18421 atcaaatgct gcaaagtcag cctaatgcca aagtaaaata tgtaagcagt gaaacttttg
    18481 ctaatgattt catcaattcg atccaaaaca aaacagcaga agagttccgt caagaatatc
    18541 gaaatgttga tttactgcta gtggatgaca ttcaattttt tgcagaaaaa gaagctacgc
    18601 aagaagagtt tttccatact ttcaatgcct tgtacaatga agggaaacaa atcgtactaa
    18661 ccagtgatcg gttgccaaac gagattccga aactgcaaga acgcctcgtt tctcgctttg
    18721 catgggggct gtctgttgat attacaccgc cggatcttga aacaagaaca gcgattttac
    18781 gtaaaaaagc ggcggctgaa cgtctagaga tcccagatga cacactaagc tacatcgcag
    18841 gacaaattga ttctaacatt cgtgaattag aaggtgcgct tgttcgagtg caagcttttg
    18901 ctgcgatgaa tagtgaagat atttcgacta gtctagctgc agacgcactg aaaacgttaa
    18961 aatcaggaaa aggacatcct caattgtcaa tcctgcagat ccaagaagaa gtggcaaagt
    19021 attatcacat ccaattgaaa gatatcaaag gaaagaaacg cgtcaaatct attgttgttc
    19081 ctagacaaat cgctatgtat ttggcacgtg agctcacaga taattcttta ccgaaaatcg
    19141 gagctgaatt cggtggcaag gatcatacaa cggttatcca tgctcatgaa aaaatccagc
    19201 agctgatgga ttctagtgtc agtatgcaaa atgaagtatc agaaatcaaa aactttttac
    19261 taaattaatt tgtcgattct atcaactgaa aatagcatgt ggataaaaga aaaaaacgtt
    19321 caaaagttat gcacactttt tccacagctg ttttttcgtt tctctcttac gttttttgag
    19381 ttttccacag aatcaacatg ccctattact tttattattc tttttaacta ataataaata
    19441 aacaaacatt caaaggagat acatcatgaa agttacttta aaccgagcta gctttatgca
    19501 ggaattgcaa actgttcaac gagctatttc aagcaaaacc acgatcccta ttttgacagg
    19561 tgtaaaaatc acactgacac aagaaggttt gactttgacg gggagcaacg ctgatatatc
    19621 aattgaaact tttttgtctg ttgaaaacga aaaagcaaat atgcaaatcg aatctactgg
    19681 ttccattgtt ttacaagcac gtttctttag cgaaatcatt cggagacttc ctgaagaaac
    19741 atttacttta gaagttttag aaaataaaca agtagcgatt acttctggaa aagcgaattt
    19801 tatcgtaaat ggattagatg cagataacta tcctcatctt cctgttgtcg aaagccataa
    19861 ccagatgaaa ttacctgtac acgtattgac taaactaatc aacgaaacag tttttgctgt
    19921 ctcacaacat gagagtcgtc caatcttgac aggtgtgcat ttcattttat ctgataattc
    19981 tttattggct gtagctactg attctcaccg tctaagtcaa cgcgtgattc cagtagaaca
    20041 agcggctgat cattttgata ttgttattcc tggaaaaagt ttgatcgaat tatctcgctc
    20101 attaacaaat gaagaagaaa tcgtcgaaat cagcattatg gaaaaccaag tgttgttcaa
    20161 aacagaaacg atgtatttct attcccgttt gctagaagga aactatcctg ataccaatcg
    20221 tttgattcct tcaagtttta atacagaggt tgaattttct gttcctagct ttttagcggc
    20281 gatcgaacgg gcttctttac tttctcatga aggccgtaac aacatcgttc gtttatctat
    20341 tcgtccagat gcggttgttt tatacggaaa ttcgccagaa atcgggaaag tagaagaaag
    20401 cttgagttat acagcaagca gtggcgatcc attagatatt tctttcaatc cggattatat
    20461 gaaagcagcg ttgcgtgcat ttggagatat gagcatcaaa gtgaaattta tctctgctat
    20521 tcgtccattt acattggaac caacggaaga tggcgttcaa tttattcagc tgattactcc
    20581 tgtacgta
//
LOCUS       DAYYHX010000035        20282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000035 DAYYHX010000000
VERSION     DAYYHX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.035"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(84..1643)
                     /locus_tag="ACHA6Y_000932"
     CDS             complement(84..1643)
                     /locus_tag="ACHA6Y_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM8987045.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1798..4032
                     /gene="pcrA"
                     /locus_tag="ACHA6Y_000933"
     CDS             1798..4032
                     /gene="pcrA"
                     /locus_tag="ACHA6Y_000933"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM8987046.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4049..6085
                     /gene="ligA"
                     /locus_tag="ACHA6Y_000934"
     CDS             4049..6085
                     /gene="ligA"
                     /locus_tag="ACHA6Y_000934"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM8987047.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6191..6496
                     /gene="gatC"
                     /locus_tag="ACHA6Y_000935"
     CDS             6191..6496
                     /gene="gatC"
                     /locus_tag="ACHA6Y_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM8987048.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6496..7968
                     /gene="gatA"
                     /locus_tag="ACHA6Y_000936"
     CDS             6496..7968
                     /gene="gatA"
                     /locus_tag="ACHA6Y_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM8987049.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7965..9395
                     /gene="gatB"
                     /locus_tag="ACHA6Y_000937"
     CDS             7965..9395
                     /gene="gatB"
                     /locus_tag="ACHA6Y_000937"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM8987050.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9405..10460
                     /locus_tag="ACHA6Y_000938"
     CDS             9405..10460
                     /locus_tag="ACHA6Y_000938"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8987051.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10571..11944
                     /gene="rlmD"
                     /locus_tag="ACHA6Y_000939"
     CDS             10571..11944
                     /gene="rlmD"
                     /locus_tag="ACHA6Y_000939"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8987052.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11947..12075
                     /locus_tag="ACHA6Y_000940"
     CDS             11947..12075
                     /locus_tag="ACHA6Y_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8987053.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            12515..12703
                     /locus_tag="ACHA6Y_000941"
     CDS             12515..12703
                     /locus_tag="ACHA6Y_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM8987054.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12781..13794
                     /locus_tag="ACHA6Y_000942"
     CDS             12781..13794
                     /locus_tag="ACHA6Y_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8987055.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(13861..14895)
                     /locus_tag="ACHA6Y_000943"
     CDS             complement(13861..14895)
                     /locus_tag="ACHA6Y_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8987056.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            15010..17235
                     /locus_tag="ACHA6Y_000944"
     CDS             15010..17235
                     /locus_tag="ACHA6Y_000944"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8987057.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            17253..19136
                     /locus_tag="ACHA6Y_000945"
     CDS             17253..19136
                     /locus_tag="ACHA6Y_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM8987058.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            19117..20091
                     /locus_tag="ACHA6Y_000946"
     CDS             19117..20091
                     /locus_tag="ACHA6Y_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM8987059.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         6838 a         3719 c         4343 g         5382 t
ORIGIN      
        1 ccagcctctt ctttttttat agaatcatat agaatcaagt agtgtagatg aatagttgtt
       61 gtaaaatagt ttttccatgt tcgttaaaat gattgcttca aatttgtgat agaagattta
      121 gcctcgattt cctgcttgat tcttggcaag tttgcttcat cagtcaagat aaccaaaata
      181 tctcctacat tcatgatcgt atcaccgtga gtcaagattt cctgatttcc tctgcgtata
      241 gagattagca gcatattttt aggccaattg aagtctcgta ccattaatcc gtctaaagaa
      301 ctctctgccg ttactggaaa atcaaaagtt gtccttgcac cttttagtga ggcggtctga
      361 gtatttccaa gtagacgttc caataatgat tcgtaaattg gattaccgcc gagaaggtca
      421 ttgacgatat aagcaacaag agcagccaat cctaaaggca tcagatgatt aagagagcca
      481 accatttctg tcactaaaat aattgctgtc agcggtgctt ttccaatcgc tgtgaaataa
      541 ccagccatgg caataacgac aaaatccttg atgtagattg gatccatccc gaaatcaacc
      601 ataatgtttc cataaaagct accgattaaa gcccccaatg tcaatatcgg aaggaaaatc
      661 ccccctggaa gacccccacc gtaagaaatc atggaaaaaa cgaatcgaag aacaaacaaa
      721 ccgataatca taatgattgg caaggaagca tgtccaaaag ctaaaacgat ttgattgcct
      781 ccgcctagag tactaggcca taaataacca attggcaaaa tcagcaagaa aggaacgaac
      841 ccgtgaaaat aagctggtaa aggcaatcgg ttgtaaaatt ttggcaaagc cagcaagaca
      901 ttctgataaa taaaacctaa gatgcctaga agtactccta agatcactaa ggaaccataa
      961 tatttcaacg gcaaagaagg aaggtcacct agatataaaa caggtctcaa gccgaaaaag
     1021 taaagagaga cgaagttagc agtgattgct gaagcaaatg atgtcagcca aacaagtgga
     1081 gaaaaggagt gatggatttc ttctaaaaca aagagtaatc ctgcgatagg agcattgaat
     1141 gctgctgcaa gtccgctact tgcaccgcta gaaattaaaa tcttttcttc tgaagatgga
     1201 ctccgaaaca attgtccagc tccttgtcca atggatgcgc caagctgtat agaaggtcct
     1261 tctctaccta gaaaaaggcc cgcaccaact gaaagtacac cgccaatgaa ctttttccat
     1321 aaaacagaga accagttcat tgataattcc ccgcgaagct gtccctctac ttgtggaatc
     1381 ccgcttccct tgatatccgg ttcatttttg accagataac ccacgaaaaa tgctgcaagg
     1441 atgcagcaaa tagctaggcc aattaagtaa atcggctgtt cccgactcat ttcatagata
     1501 cttcgaacta aatcgcttag cttttcgata gataatcgaa acaggctaac gacgattcct
     1561 gtcagcagtc caacaaatat tccttttaga ataaaatata tctgagtctt atctaatttc
     1621 ttgatttttc cttttttttc cattcaaacg cctcgctttc ttttaattta gcgcgaaatc
     1681 aacgaattga caaggaaaaa tccataaaaa gaaacaaatg attttgaact agctagttta
     1741 atgaaagcca agaaaatgct ataattaaca agattgataa aagaaaggat gttgttaatg
     1801 tcatccaaag ctgaattatt aaacgggatg aacccccgtc agaaagaagc tgttcttcac
     1861 actgacggac cattactgct aatggcaggt gcaggaagtg gaaagacgag agtgctgaca
     1921 caccgcattg cctatttgat tgaggaaaaa gaagtcaatc cttggaacat cctagcgatt
     1981 acttttacca ataaggctgc aaaagaaatg aaagaacgtg tcaatgcgat tctcgcatct
     2041 ggaggcgaag atgtctgggt atcgactttt cactcgatgt gtgtgcgtat tttaagaaga
     2101 gatgttgatt ttatcgggta taaccggaac tttacaatca tcgattcttc tgaacagctg
     2161 accttgatga aacggatact aaaagaactg aacattgatc cgaaaaaata tgatcctcga
     2221 agtatattag gaacaatctc tcaagcaaaa aacagcctgc agacccctca ggattttgca
     2281 aaaatgcaag gcagttatta cgaagagatt gcggcaaaat gttatgctgc ttaccaaaaa
     2341 gagcttcaat ataatcaatg tatggacttt gacgacctga tcatgaatac gattcgatta
     2401 tttgaagaac atcctgattc actcacgtat tatcaaaaca aattccatta tattcatgta
     2461 gatgagtacc aagatacaaa tcacgcacag tatacgctag tcaatttatt ggcaggtaga
     2521 tttaggaacc tgtgcgtcgt gggagatgca gatcaaagta tttacggctg gcgcggtgca
     2581 gatatgcaga acatcttgga ttttgaaaaa gattatccag atgctgcagt aattttgcta
     2641 gaacaaaact atcgctccac gaaaaacatc cttagcgcag caaaccaagt cattgaaaac
     2701 aatagcaatc gaaaaccaaa aaacttgtgg acagaaaaca aagaaggaaa caagatcact
     2761 tactaccgtg cagataatga acgagacgaa acccgtttta tcgtagaccg gatgcaagaa
     2821 gagattcgaa gcaatcatcg gaattacggc gactttgcca tcttgtaccg gacgaacgca
     2881 caatctcgtg taatggaaga aactctgttg aaagcaaata ttccttataa aatggttgga
     2941 gggcacaagt tctacgatcg taaagaaatc aaagatattt tagcgtattt gaatgtgctg
     3001 gtaaatcctc aagattcaat cagttttgag cgtatcgtta attcgcccaa acgaggcatc
     3061 ggaccaggat ctatcgaaaa actacgcagt tttgcttctc ttcatgaatg gccactgctt
     3121 gaagcagcgc aaaatgtgga tctggcaaat atcggtggaa aagcaggaca gcaattaggt
     3181 gcatttggag agatgatcca agaagtcact cagatgatcc catacttgac agtgacagaa
     3241 ttgacgaaag aggttttgga tcgtagcgga tatctagaag acttaaaaat ccaaaataca
     3301 ttagaagcac aagcacgcat cgaaaacttg gaggaatttt taacggtcac acaagaattc
     3361 gacaaacaat tcgaacagca aaatgaagaa gacgctgatg caccagaaga aaaactaacg
     3421 gtcttcttaa atgatctggc acttgtttcc gacattgaca atttagaaga agatgcttcc
     3481 caagtgacat tgatgacttt gcatgcagcc aaaggattag aatttccagt tgtattcctg
     3541 attggtctag aagagggggt ttttccttta tctcgagcat tgatggaaga aagcgaactg
     3601 gaggaagaac gccgtctcgc atatgtgggg atcacgcgag cagaagaagc gttgtattta
     3661 acaaatgctt tctccagaac attatatgga cggacgcaat ataaccgacc ttctcgtttt
     3721 gtagaagaaa tcgatcagga gttactggaa atcgaaggca tgcgtcctac gcctaaaaaa
     3781 acgcctgtat tcgctaaaaa aacagcatat agctataaac agccagagac agcggttgtt
     3841 ccctctaaaa gtgctactgg aggggaaaat aacagctgga aacctggaga taaagtcaaa
     3901 cacaaaaaat ggggtctagg aacagtcgtc cgagtaagcg ggacgtcgaa ggatctagaa
     3961 ctagatgtgg ctttcccaag tcaaggagta aaacgtcttt tggctgcctt tgcgccaatt
     4021 gaaaaagctt agtagtggag ggtaaatgat ggaacatgaa atgacgattg acgaagctgc
     4081 taaacgagca gaagaacttc gtacaagact gaaccaatgg tctcgcgaat actacgtaga
     4141 agataaacca acagtggaag actatgttta tgataaagaa tacgcagaat tagtagcaat
     4201 cgaggaacaa tatcctgatt tgatcacgtc tgattctccc actcaacgag taggcggaaa
     4261 agtattagaa ggttttgaaa aagtcactca tgatattccg ctttacagtt tgaacgacgt
     4321 tttcagcaaa gaagagctga tagcttttga tcaacgtgta caaaaagcag tgggacgtgt
     4381 agttgactat tgctgtgaac taaaaataga tggactttcc gtttctttgc gttatgaaga
     4441 tggaaatttc gtgagaggtg cgactcgcgg ggatggaact gtaggagaaa acatcactga
     4501 aaacctaaaa actgtccggt cggtcccaat caaattaaaa gaaccaatga acatcgaagt
     4561 aagaggcgaa tgtttcatgc caaaacggtc atttgtccaa ttgaaccaag acagagaagc
     4621 agagggaaaa gatatctttg cgaatcctcg gaacgccgca gctggttctt tgcgtcagtt
     4681 agattctaaa atcacagcga aaagaaatct ggacactttt ttatacacag tagctgattt
     4741 cggtcctatg caagcaaaaa cccagtatga tgcgctagaa gagctagaaa aaattggatt
     4801 ccacacgaat cgggaaaaac gtctatgtca ttcgattgat gaagtgtggt catacattga
     4861 agaatatcat gacaaacgtg tggatttgcc ctatgaaatc gatggaatcg tcatcaaagt
     4921 gaatgaattc tcgctacaag atcaacttgg ttttactatc aaagcaccga gatgggcagc
     4981 agcttacaag tttccgccag aagaagttga aacgctaatc gaaaacatcg aatggacagt
     5041 cggtcgaaca ggggtagtaa cgccaacagc tatcatgaca ccagtccgag tagcaggaac
     5101 gacagtaagc cgtgccagcc tgcataacgg tgattatatt aagttgaagg atatccgctt
     5161 gaaagataca gttctgattt ataaagcggg agacatcatc ccagaagttt cgcaagtcgt
     5221 gttagataaa cgacctaaag acagcgaaga ataccaattg cctacacatt gtccagtttg
     5281 tggaagtgaa cttgtccatt tggatgaaga agtagcctta cggtgcatca atcctaaatg
     5341 ccctgctcaa atgaaagaag gtttgaacca ctttgtttca agaaatgcga tgaatatcga
     5401 tgggttgggg ccgcgcgtac tagaacagat gtatgataaa aaactagtag cagatgttgc
     5461 tgatctttac aaattgacag aagaagagtt attgacgttg gacaaaataa aagaaaaatc
     5521 agcaaataat attttgacag caatcgacaa cagcaaagat aattcggtcg agcggttgat
     5581 tttcgggtta ggaatccgac atgtcggagc gaaagcagca aaaattttag cagaacattt
     5641 cggtgatcta gaaacattga gtaaaagtga ctatgagtcg attattgcgc ttgatacgat
     5701 cggtgatatc attgccgaca gtgtagtcac ttatttcagt aacgaagaag tccatgaatt
     5761 gatgaatgaa ttaaaacaag caggcgtcaa tttcgaatac aaagggcttc gaaatgccca
     5821 attgcaagaa gtcgagtctc cttttaaaga aaaaacagta gtccttactg gtaaactaac
     5881 acgatttact cgtgaagaag caaaagagac gatcgaaaat cttggcggga aagtgactgg
     5941 tagcgtatcg aaaaaaacgg atatcgtggt agcaggagaa gatgctggca gcaagctgac
     6001 caaagcacaa gaattaggaa tcgaagtttg gacagaagat caaatggcag atgcattggc
     6061 taaaagtcgc tcagtagaag aatgaaaaat gagttttttc agacaaattt caataaacaa
     6121 tttcagacac ggcactcttg tgttaaaata gagagcaaag tttttaactc gtttagaaag
     6181 aaggggtagt atggcgatta gtgaagaaca agtgaaacac gttgccaaat tagcaaaact
     6241 tgcgttcgca gatgaagaac ttgcaagttt taccgaccaa ttaggtaaaa tcatcgatat
     6301 ggttgaaatg ctggaagaag tagatacgga aggtgttcca tttacgtcga atgtggttga
     6361 aacagtaaac gtgatgcgac aagatcgggc gcaagctgga tggaaccgcg atgaattatt
     6421 gaaaaatgtg ccggaaacag aagacggctt tatcaaagta cccgcaatca tcgataatgg
     6481 ggaggctggc gcataatgac agaattacac aatcgttctc tagaagaact gcatcatatg
     6541 cttgtatcga aagaaatcac tgtacaagat ttgacaaaaa caacatttga acgaatcaaa
     6601 gaaacagagc ctgaggtcga ggcattcatt acattgaacg aagaaaaagc attggaacag
     6661 gcaaaagctt tagatgaaaa agggattgat gaaagcaacg tccttgctgg tattccaatt
     6721 ggtatcaagg ataatatcgt aacaaaagat atcttgacaa ccgctgcatc taagatcctt
     6781 tacaattttg aaccaattta tgatgcaacg gtcatggata aaatctatca agcagatatg
     6841 atccctgttg gtaagctaaa catggatgaa tttgctatgg gtggaagtac agaaacttct
     6901 tatttcaaga aaacaaaaaa tgcttgggac ccaacaaaag tacctggtgg ctcttctggt
     6961 ggttctgcag cagcagttgc agctggacaa attccagttt ctttaggtag tgatactggg
     7021 ggaagtatcc gccaaccagc agcttttaac ggcatcgtag ggatgaaacc tacttatggc
     7081 cgtgtctcac gttttggttt gattgccttt gcttcatctc ttgaccaaat cggaccaatg
     7141 acccgtacgg tgaaagataa tgcattagca ttgacagcta tcagcggtta tgacgaaaaa
     7201 gacggtactt cttcaggcgt ttcagttcct gattttgcag agggactgac aggcgacatc
     7261 aaaggaatga agattgcttt acctaaagaa tacttaggcg aaggtgtggc ccctggcgta
     7321 aaagaagctg tattgaaagc agcagaaaca ttccgttcat taggtgctgt tgtggaagaa
     7381 gtcagcttgc ctcattcaaa atacggtgtg gctgtttatt atatcattgc ctcatcagaa
     7441 gcaagttcca atttgcaacg attcgatggt atccgctacg gttatcgctc tgaaaatgtg
     7501 aaatcattag atgatgtata tgtcaactca cgttcagaag gattcggtga agaagtaaaa
     7561 cgccgtatca tgttaggtac attctcctta agcgcgggtt attatgacgc ttacttcaaa
     7621 aaagcaggac aagtccgtac attgatcaaa cgagattttg aaaaagtctt tgcagattat
     7681 gacttgatca tcgggccatc ttcaccaaca gttgcattcg gaatcggtga gaacattaac
     7741 gatccaatca caatgtacat gagtgacatc ttaacgatcc ctgtcaactt ggctggactt
     7801 ccaggtatgt ctgtaccggc aggtttatca gaaggattac cagttggatt gcagatcatc
     7861 ggtaattatt ttgatgaaaa aacgatgtat caagcggcat atgcgtttga acaagcaaca
     7921 gaattccata aacaacaacc agctatttta ggagggaaag acgaatgaat tttgaaacag
     7981 tcattggact agaagtccac gtcgagttaa aaaccaactc aaaaattttc tctccagcgc
     8041 cagctcattt tggtgcagaa ccaaacagca atacaaacgt gatcgactgg ggttatccag
     8101 gcgtgctgcc agtaatgaat aaagctgcgc tggaattcgg gatgaaagca gctttggcat
     8161 taaactgtga aatctctaaa gatactcatt ttgaccggaa aaattacttt tacccagata
     8221 atccaaaagc atatcaaatt tcccaatttg atcaaccaat cggtcatgac ggatggatcg
     8281 aaatcgaagt agaaggtaaa aagaaaaaaa tccggatcga acgtgtccat ttagaagaag
     8341 atgctggaaa aaacatgcat ggtattggcg gttattctta cgttgatttg aaccgacaag
     8401 gcacgccttt gatcgaaatc gtttctgaag ctgatatgcg ttcaccagaa gaagcatacg
     8461 cttatctaga agcattgcga tcaattatcc aatttacaga agtcagcgat gtgaaaatgg
     8521 aagaaggctc tatgcgctgt gatgccaata tttctcttcg cccttatgga caagaagagt
     8581 tcggtacgaa agcagaattg aaaaacttga actctttatc tttcgtgaaa aaaggtttag
     8641 cttttgaaga aaaacgtcaa gcaaaagttc ttctttcagg aggagaaatc caacaagaaa
     8701 cacgccgttt tgacgaaaca acgaataaaa cagttttgat gcgtgtcaaa gaagggtcta
     8761 gtgattatcg ttacttccca gagcctgata tcccgaaatt cgtgatcgat gacgaatgga
     8821 ttcaaaaagt aaaagctagt ttgcctgaga tgccagcttc tcgtcgcgaa cgctatatcc
     8881 gtgagtttgg acttccagag tatgacgcaa tggtattgac acttacaaaa gaaatgtctg
     8941 atttttttga agctgttttg gaaaatggtg cagatgccaa gcaagcatct aactggttga
     9001 tgggtgaagt ttctgcttac ttgaacagtg agaaactgga attagctgag acaaaattga
     9061 cacctgagaa cttagcagga atgattcgtt tgatcgccga tggaacgatc agctcaaaaa
     9121 tcgccaaaaa agtcttccgt gaattgatca caaatggcgg agaagcccaa gaagtagttg
     9181 aaaaaaatgg ttgggtgcaa ttatctgatc cgaacaaact actgccaatc attaatgaaa
     9241 ttttggataa caaccaacaa tctgtcgatg atttcaaaaa tggaaaagat cgtgcggttg
     9301 gcttcctagt cggtcaaatc atgaaagcaa ctaaaggaca agcaaatcct ggtgtcgtga
     9361 ataagctact taaagatgaa ttagcgaaac gatagaggct gaaaatgaaa aaagcacgcg
     9421 taatttataa ccccacctcg ggaaaagaat tattgaaaaa aaatttggcg gatatcctac
     9481 aggcattaga agaagcagga tttgaagcca gcgcctatgc aactacacca gaaccagatt
     9541 cagccaagaa tgaagcatgt agagtcgcag aactaggttt tgatctagta gtggcagctg
     9601 gtggcgatgg cacaatcaac gaagtggtta atggaatcgc tccgcttgaa caccgtccta
     9661 aaatggccat catcccagct ggtacaacga acgactatgc tcgagcatta aaaattccta
     9721 gagataatat taaagcagca gcagaagtga taaaaaaaca ccaaaccgta aaaatggaca
     9781 tcggcctttc tgataacagt tacttcatca atatcgctgc aggtggctat ttgacagaat
     9841 tgacttatga agttccatca gaactaaaga gtatttttgg ttatcttgct tatcttgcga
     9901 aaggagcaga gatgctccct cgagtaaagc caatcaaaat gcgcttgaag tatgatgaag
     9961 gggaatatat cggaaatgct tcgatgtttt tcctagggct gacgaactct gttggaggat
    10021 tcgaacagat cgcgccagat gccaaattgg atgatgggaa gttttcttta atcattgtga
    10081 agacagcaaa tatctttgag atcttgcata ttgcagcact catgctgaat ggcggtcgac
    10141 atgtcgatga tccaagaata atctatacca aaacaagcag tttgttcgtt gaaacagaag
    10201 atgatatcaa tcgaccagta atgatcaact tagatggtga atacggcggg gaagcaccga
    10261 tgcatttcca aaatcttcat caacacattg agttctttgc caataccgat cagattcctg
    10321 atgaagcaat cacaggtaca gatgaagaag aattagaggt tgtcagcaaa gaattcgtaa
    10381 aagaagtcga acgtttaacg gatgaagata tcgacggtga cggaaaaata gcagacgaaa
    10441 cacctaatca aaagaattaa tcaaaagccg gtgctgaacg tagtaaggtc agtgccggtt
    10501 ttagttgtgt aattagctga tttatagtag agttcagtag gattgaaaaa taggcttaaa
    10561 ggagcaaaat atgacagaac agcaaatagt aaagaaaaac gaacgatata cagtcgaaat
    10621 cgtcgacttg agttatgaag gaatgggggt agcaaaaatc gatggttatc cattatttat
    10681 cgaaaatgca ctccctggag aagaagtaga aatactagta gtaaaagcag gcaataagtt
    10741 tggatatgga aaagtcgaaa catttattac acaatcacct gatcgtcagc ctgtagaaaa
    10801 caatgcatta cttcgcatag gtatcgcacc tttagcccat ttgaagtatg aacaacagct
    10861 tgtcttcaaa caaaaacaag tagagcatgt aatggcgaag attgctaaga tgccagaagt
    10921 tcctgtactt ccaacggtcg gaatggcaga accattcggc taccgtaata aagcacagat
    10981 ccctgttcgc cgtatcgatg gacagttagc caccggtttt tacaaaaaga atagccatga
    11041 tctagtccct atcgaagatt tttatatcca agatcctgtc atcgatcaag caatcaaagt
    11101 agtcagagat atcttgcaac ggttccaagt acgaggatat aatgaagaaa aaaatgaagg
    11161 acagcttcgt cacatcatta tccgtcgtgg tcattattcc catgaaatga tggtcgtttt
    11221 agttactcgt aaagaaaaat tctttaaatc caaggaaatt gctgaagcta ttcatgaaga
    11281 attgccagaa gtggtttcgg tcatccaaaa tatcaatgaa gaaaaaacaa atgtgatttt
    11341 aggagacaaa gaaaaagttt tatatggcag agactatatc gaagatcagc tattaggaaa
    11401 aacctatcga atctcagcaa agtcatttta tcaagtaaat actcaacaag cagagtatct
    11461 gtaccaaaca gccatcgagt ttgccgagtt agataaaaaa gacattgtga tcgacgctta
    11521 ttcaggaata ggtacgattg gcttgtcttt agcagacaga gtggataagg tgtatgggat
    11581 ggaagttatt ccagaagcaa ttgaagatgc acagttcaat gctttaaata ataaaatcga
    11641 aaatgttcat tatgaagtcg gaaaagcaga aaaagttatg aaaaaatggc aagaaaaagg
    11701 gatcaaacca tctgtgatcg tagttgaccc tccacgaaaa ggattagatc atcattttat
    11761 tgaatcagca atcgaaatgt caccagaacg aatcgtctat atctcatgta atccggcaac
    11821 ttttgcaaga gatgctaagt tatttgcaga agcaggttat gccgtgaaga aagttcagcc
    11881 tgtagatctt ttcccgcaaa cccatcatat cgaattagtc gcttcattcg ttaaaggaga
    11941 ataaaaatgg accaaaaaga aaaaatgaaa gaattgatca aaaagaaaca aggtggaggc
    12001 cgatccaaac aaatggaaac acctaagaat gatcgtaaaa acatgagaaa aggccctaaa
    12061 atctttaata aatagacgtg ctaaagaaag actaagaagt gttaactttt gttattgttt
    12121 cttagtcttt ttagttagtc tacattattt tggcgatttg ccaataagga ctgttgagtt
    12181 gtgcaaaatc aaatctgggt cagaatgaac gtcattctgt ctcagatttt tcgttatttc
    12241 aatttgatta actgattgat caaaaagatt tttattttca tattctcaaa tgattcaaca
    12301 ctaaatatac atgtttgacg cggaaattat tattcgattt tggcggcatc aggggggatg
    12361 acgacgtagc agacccatta ttttactcaa gaaaatgtcc gtgcctatta tttagaatac
    12421 gatagtgaac gtgctgggag ttttgttctt ttagagaaag tctctgaaga taaattagtt
    12481 gtcttagatt ttgactgtaa cgaaaaatgg cgaaatggaa gagcaccagc aagtgattga
    12541 ccggatctac gaagcgacac gatatctgcc tttggatcgt ttgtgcttaa gtcctcaatg
    12601 tggatttgct tcaacagaag aagaaaatgt tttgtttgaa gaacaatgga aaaaatttct
    12661 tttgtcaaaa gcattgcaga agaagtttgg ggaaaatcat taaatcatct aggagagctt
    12721 gataggaaat aacactatgg tataatcaga atgcttaata ctaactactg agggagcctt
    12781 atgaaaaaga aaattcttaa atttatcgga tggttcttcg gaatcattct tgggatcgcg
    12841 tttgcaatct ttcttgcctt ccagctttcc cccagacctg gagcattctt gatcaaccat
    12901 atgttttcaa atactgttca aatcactgat aaaaaaacgt tcgatcaagc agaaaaaaac
    12961 gtggcgaaaa aaacagattt gacgtatgag tccaagtata aagagaatac ttatgacatc
    13021 tactatccaa aaaacagcaa ggggcctgtt cctgttctat tttgggtaca tggtggagga
    13081 ttcgtcggcg gagataaatc gggtgtcaaa gaatttgcga caaaattagt ttcagacgcc
    13141 aacatcgcag tggttgcgat gaattatgaa ttagcgcctg attcagaata ccctaatcaa
    13201 gttcttcaag tgaatgaact gatcaaagat ttattagctg aagaaaaaga cgacaaattg
    13261 ctagacatgg aacaattatt ttttggtgga gatagtgcag gttcacaaat cgcattgcaa
    13321 tatgcagcta tacaaacgaa tgatgcgtat gctaaacaaa tgaaaatcaa acagctactt
    13381 ccaaaagata gtctgaaagg aaccatttct tattgcggac ctgttgattt gaagctaaca
    13441 gcacagcagc attcggatga tcgatttatg aagtttttcg tcaaaaccgt tgcgtggtcg
    13501 ttgattggaa caaaagattg gaaaacgagt gagcaattga aagaagctag tgtggctgac
    13561 catgtttcta aggaatttcc gccaacgtat ataactgacg gaaatgcata ctcattccag
    13621 gagcaaggtc aagcattaga aaatcggttg acagaattaa aggtacccgt gcaatccttg
    13681 ttttatacag atacaaaaaa agagattacc catgaatacc aatttgatta cacgctaaaa
    13741 gaatcgcaaa attgttacca gcagacattg gattttgtga ataagtataa gtaagaggtt
    13801 gtgaaaaagc cgtttcgttt tgagtatgaa gaaacgaagc ggcttttcac aacctctctt
    13861 tcaatacctt tttgttcttg ggataaaact ttccctaaca atcaatcgtg caggataagt
    13921 caaaatgatt ggttcgtccc gtataccgtc aattcgctcc aatgccattc gaacagctgc
    13981 ttgcccaatt gatttcgcat tgatttgaac actggtcaag ccaggggaga ggtttgcgat
    14041 tgtctcgata ttatcaaagc ttgctacaac aatctcttct ccaatttgga tatttttttg
    14101 tttcaatgcc cgatagacac caattgccat tggatcgctc ccgacaagaa tggcagtagg
    14161 aagaggggat tcattttcta atagattagt cgcaagtacc tcaccttgtt tttctgtcca
    14221 gccacctaga tgataagaca tatatttttc taagtgatga tcgatcataa aatgctgata
    14281 ggcacgcatc ctcttttcat ttatagaaat ctgcttccca ccgaattcat ccatatcgac
    14341 tttatatccc ccgatatagg ctattctttt atgaccattt tctaagaaca attccagtac
    14401 ggttttagtc atccgttcaa gatctgcata caccatatcg acctcttgct ggatgtcagg
    14461 gttatccacg acgactattt gcatgttttg ttttaacaag gcggtaatcg attctttcgt
    14521 aacagtccca acaacgatca ccgcacctaa ctgatctaga tcttcccatt gtttaggatt
    14581 atcagataga ttatagattc gattcatccc taaatgcaga cgtcgagctt cttcttccat
    14641 ccctactcgc aaatgtttgt aatagatatc ctgttcttcg ctcgctttgc cgatcgtaga
    14701 gatcacacca atcgaaggca ttctcttttt gatatattta cgagtactga tgtcataatc
    14761 caacgcagca gctgcctcta ctatccgttg ccgagtagtc tgagccacat ggatcgtctg
    14821 atcgttattc aacacacggg aaactgtagc aggagagaca tttgcctttc gtgcgacatc
    14881 tctaattcca accattttta cacactcctc aaaaaaataa aaaattttta ttgacaaaat
    14941 tgtttagtaa acagtataca ggaattataa aaaaaagtaa acggtttccg ttttgttggg
    15001 agtgaaatga tggatattac gcatagaaaa acagataaaa atttcatctt atttgatgaa
    15061 aagagccgag tcttccattt acgaaataca tttctttctt atctgataaa gatagaagaa
    15121 agcaatgtat tggcgcatat ttattttggc aaaccagtca agcagtacaa agacaataaa
    15181 aattatcccc gaagagatcg tggattttca ggaaatgttc ccttaaatcc tgaccggtct
    15241 ctgtcgaaag atacactacc gcaagaatac tcttctcatg gcagcatgga ctttcgaacg
    15301 cccgcatcga tcattcagcg caagaatgga tcagacttac tggatctgag atatgacagc
    15361 cattatatta cagatgggaa gccagagatc gaaggcttgc ctcaaactta tgtgatggac
    15421 aagtcagagg cacaaacgtt ggtgatttca cttaaggata gagaaacagc gatttatttc
    15481 gatttgttct ataccatatt tactgataga gcagtgatca cacgatctgt aaagataaga
    15541 aatgaaaccg gagaaacaat caaactagaa aaagcggcta gtttccagct agactttgcc
    15601 catactaggc gatttgatga agtcattgct ttgccaggag cacatgtcaa tgaaagacag
    15661 atttcacggc aatctgtttt atctggaaca aaagtatttg aaagccgtcg aggaacgagc
    15721 agtcaccata tgaataactt tattgcttta gttcatcatc atacgacaga aaatactgga
    15781 gaagcgatcg gacttcaatt tgtttattcc ggcaaccatt ctttcgaact tgaaaaagat
    15841 cagatcaatc aattgagagt cgtcggtggg atcaacagcc atcgtttttc gtgggaattg
    15901 aatgcaggcc agtcttttca aacacctgaa atgatccttt cttactcaag tcagggattg
    15961 aacaaaatga gtcagatcca ccatgagttg ctacgtgaaa gaatcgcaag aggtcgtcat
    16021 caatttgctg aacggccgat tctggtcaat aactgggaag ccacttactt tgacttcaat
    16081 agtgaaaaaa tcaaggcgat cattgatgaa gcaaaggagc taggcatcga aatgttcgta
    16141 ttagatgatg gctggtttgg aaaaagagat gcggacaatt cttcattagg agactggttc
    16201 gagtatgaag gcaagttgac aaatggatta cgtgaaatag cagattatgc tcatagcaaa
    16261 ggattgaaat ttggtttatg gtttgaacct gaaatgatct cggttgattc tgaactgtat
    16321 cgaacacatc ctgattttct tatgcaggag ccaggtagaa tgccttctgc ttccagatca
    16381 caacatgtac tggattttac aaggttagat gttcggcaga cgattgagaa acaaatgaga
    16441 aagattttag atacgattcc tcttgactac atcaaatggg atatgaaccg ttcgctttca
    16501 gatgtctatt cgattaccct tgacccacag agacaaggag aagtagcgca tcgttatatg
    16561 ctcgggcttt atgaactact tgaacatttg acaactgatt atccggaaat cttatgggaa
    16621 ggctgttctg gtggaggtgg acggtttgat gccgggttta tttattatat gccgcagtca
    16681 tggacaagtg ataacacgga tgcagtggag cgtatgaaaa tccaatatgg aacaagcctt
    16741 gcttatccga tttcgtccat cacagctcat gtatcagctg taccaaatca tcagaccgac
    16801 agatctactt cgttgaaaac aagaggagaa acagctatga gcgctgtttt tggttatgaa
    16861 ctggatttga caaagctgtc tcctgaagaa aaaaagcaag taaaagacca aattatatcc
    16921 tatcaaacga ttcgtccggt gatccagtat ggccattatt atcgtttggc gagtcctttt
    16981 gaagaaaata ttgccgcatg gatgtttgtg tctcctaaac aagatgaagc tattgtgttt
    17041 ttagggagga tactagcttc agctcaacca gcgttccatg aagtatatct gatggggtta
    17101 gatgatgagg cactttatca ggaacagacc tcgaagcgga tattttcggg ggccgaattg
    17161 atgacagttg gactttactt ccccgatttt caaggtgatt tccaaacaga actgcttcat
    17221 ttcaaaaagt tatgagagag aaggaaaaaa gtatgaaagc aaatataaaa tggttagatg
    17281 atccacaagt ttttagagtg aaccaatcaa atgcacatag cgatcatgtt tgttatgcaa
    17341 caagggaaga agtagaaacc acatcctccc ttaaacagtc tttaaatgga tcatggcgtt
    17401 tttgttattc gccaaatcct tcggtacgac caaaaacatt ttataaaaaa gattttgatg
    17461 cctgttcttt tgatgaaatc actgttccta gtcatattga attagcaaat tatgacaaga
    17521 tccattacat caatacgatg tatccgtggg agggacatgt atttcgccgt ccccctttca
    17581 cgacaagccc tgagaaagag gcggaaggga tcttttcagc tgctaaagac aatccggttg
    17641 gttcgtattt gacgactttt cgattagaag aaggtctaaa gggaaaacaa atctcgattc
    17701 attttgctgg tgtagaacaa gcaatgttta tttggttgaa tggtcatttt atcgggtatg
    17761 ccgaagattc gttcactccg tctgaatttg atttaacacc ttatttggaa gaagaaaata
    17821 ttctagctgt ggaggtttac aaaagaagta cagctgcatt cttggaagat caggactttt
    17881 tccgattttt tgggatcttc agagatgtct tcttagttgc tcgaggacaa aaccatttag
    17941 aagatctttg gatcaaacct cgcttagatg acaaatgtca aaatggcaaa cttgaagtag
    18001 atttgtcttt tgagcaagta gccaaaaaca gtcagctgaa tgtagcgata ctagatcctg
    18061 atgggaagga aatctatcaa cagcaatatc cgttaaaaga gcaaatgacg attcgcatcg
    18121 atggatttga aaatgtggca ctgtgggggc attttcagcc caatttatat aagcttgaat
    18181 tggagacgat agacgaagaa aaaagagtaa cggagtatat tgactattcc ttcggttttc
    18241 gtaaaatcga aatcaaagat aaaatcatct acttcaatgg aaaacggatg attatcaacg
    18301 gggtaaatcg acatgagtgg catccgaaaa aaggacgcgc catcactata gaagatatgg
    18361 aaaaagattt ggctatcata aaaaatgcaa agatcaacgg cgtacggaca tctcattacc
    18421 ccaaccagat cacttggtat tatttatgcg accaagcagg aatccatgtg atggcggaaa
    18481 cgaacctcga atcccatggc tcatggcaaa aaatgggcgc atttgagcct tcttacaacg
    18541 taccaggtgc agtacctcag tggcaggcgg ctgttcttga tcgagcaaaa acgaatttcg
    18601 aaacattaaa aaaccatcca agtattcttt tttggtctct tggaaatgaa tcttatgcgg
    18661 aagaaaacat tcgacaaatg aacgattact tcaaaaaagt agaccccgat cgacttgtcc
    18721 attatgaagg gaattttcca aatccagcat tcgaagattc gatttcagat gtaaaaagct
    18781 gcatgtatgc gccaccagaa gagatcattg actaccttga aaataaagcg gaaaaaccgt
    18841 tcatcttatg tgaatatatg catgacatgg ggaattcgtt aggtggaatg gattcttaca
    18901 tgacgttgct tgatcgttat gaaatgtacc aaggaggcta tatatgggat tttaaagatc
    18961 aggcgatcca agtgactgat ccaatcacag gcagagaagt gatgcgctat ggcggtgact
    19021 ttgacgataa accaagtgac tatgaattct cagggaatgg gatcgttttt gcagatggac
    19081 aagaaaaacc cgccatgcag gaggtaagat attattatga aaaatacagt aaataaaagt
    19141 catatggata cggaaaaagt tgcaatcgtc ttcggcgact gtacattagg tgtcaaatcg
    19201 gggaatacgc attatatttt ttcttataca agaggcggac tggaatcgct caataaaaac
    19261 ggaaaagaat ggctataccg ggaaacactt ccagtttttt ggcgagcaac gaccgataat
    19321 gacagaggta gcggatttgg atttaaatcc ggtatgtggt taggagccgg attgtatcca
    19381 aaagtcaaag aaatagaagt actggtggac aataaaaaga tcgatttgcc gattgctcca
    19441 gtaaataatc aatattcaag taatgaatac gctgaaacgg ttgaaatcat ttttacactg
    19501 gaaatcaaca cagtaccaaa aacccaagtg acaatttcct accaaatcgc tggagagggt
    19561 aaaatgcttg tcaatatgca ttattatgga caaaaaggat taccggaatt acccgcattg
    19621 ggtttaaggt tcattatgcc aacgaaagca aaattttttg agtatgaagg cctatcaggg
    19681 gaaacatatc cggatcggat ggctggtggt gtcgaaggta tttataaagt ggcaggactc
    19741 cccgtttccc cataccttgt tccccaagaa tgcggcatgc atatggaaac gaaatgggta
    19801 gaactaacaa gaaatcaaac gttggataat acggataaag atcaagaaga tttttcgttg
    19861 cgatttgaac agcaggatac gccatttgct ttttccgctt taccattcaa agcagaagaa
    19921 ctggaacagg caacacatat agaagaacta ccgccagaaa gacgaacggt tcttacgatt
    19981 tttgcaaaag taagaggagt cgggggtatc aatagctggg gagcggatgt tgagtcacct
    20041 taccgaatca gcagcgaaga ggaccatgaa atcggcttta ttattcagta gcaatcttta
    20101 gattattatt tgaaaaacgg agtttacaag acaaagctgc tttattctat atgtcatgaa
    20161 caagagagta aaaaagttct cagatacttt tgttcatgac tattttcttc aataaaacca
    20221 attgttattt ttctgaaaaa caaagttaac tatcagaaaa ttaaaaaaag tttcataaaa
    20281 at
//
LOCUS       DAYYHX010000036        19998 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000036 DAYYHX010000000
VERSION     DAYYHX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.036"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..349
                     /locus_tag="ACHA6Y_000947"
     CDS             53..349
                     /locus_tag="ACHA6Y_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM8987060.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(993..1946)
                     /locus_tag="ACHA6Y_000948"
     CDS             complement(993..1946)
                     /locus_tag="ACHA6Y_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8987061.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1969..2724)
                     /locus_tag="ACHA6Y_000949"
     CDS             complement(1969..2724)
                     /locus_tag="ACHA6Y_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8987062.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2721..3680)
                     /locus_tag="ACHA6Y_000950"
     CDS             complement(2721..3680)
                     /locus_tag="ACHA6Y_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8987063.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3673..4623)
                     /locus_tag="ACHA6Y_000951"
     CDS             complement(3673..4623)
                     /locus_tag="ACHA6Y_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8987064.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4773..5225
                     /locus_tag="ACHA6Y_000952"
     CDS             4773..5225
                     /locus_tag="ACHA6Y_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM8987065.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5314..7581)
                     /gene="gshAB"
                     /locus_tag="ACHA6Y_000953"
     CDS             complement(5314..7581)
                     /gene="gshAB"
                     /locus_tag="ACHA6Y_000953"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM8987066.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7768..8766)
                     /gene="ftsY"
                     /locus_tag="ACHA6Y_000954"
     CDS             complement(7768..8766)
                     /gene="ftsY"
                     /locus_tag="ACHA6Y_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM8987067.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8777..9586)
                     /locus_tag="ACHA6Y_000955"
     CDS             complement(8777..9586)
                     /locus_tag="ACHA6Y_000955"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8987068.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9595..13176)
                     /gene="smc"
                     /locus_tag="ACHA6Y_000956"
     CDS             complement(9595..13176)
                     /gene="smc"
                     /locus_tag="ACHA6Y_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM8987069.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13196..13882)
                     /gene="rnc"
                     /locus_tag="ACHA6Y_000957"
     CDS             complement(13196..13882)
                     /gene="rnc"
                     /locus_tag="ACHA6Y_000957"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM8987070.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14230..16011)
                     /locus_tag="ACHA6Y_000958"
     CDS             complement(14230..16011)
                     /locus_tag="ACHA6Y_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8987071.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(16037..16951)
                     /locus_tag="ACHA6Y_000959"
     CDS             complement(16037..16951)
                     /locus_tag="ACHA6Y_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8987072.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16978..17940)
                     /gene="opp4B"
                     /locus_tag="ACHA6Y_000960"
     CDS             complement(16978..17940)
                     /gene="opp4B"
                     /locus_tag="ACHA6Y_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8987073.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17940..18884)
                     /locus_tag="ACHA6Y_000961"
     CDS             complement(17940..18884)
                     /locus_tag="ACHA6Y_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8987074.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18885..19889)
                     /locus_tag="ACHA6Y_000962"
     CDS             complement(18885..19889)
                     /locus_tag="ACHA6Y_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8987075.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5563 a         4285 c         3556 g         6594 t
ORIGIN      
        1 ctcttcggta gagtaaactt atcaaataaa agacttcttt ggaggaatca ctatgaagga
       61 atatataaaa aacatctatt ttatcgaaga aacacaaaat atcgagggtt cttatattga
      121 agtaaaaaca ctctttgtca atgaagataa aaccaaagca ttggatattt ataaaaaatt
      181 agcttcaaaa aaaacgaata gttttggatt gatattaagc gagtataaaa tcaaagcaga
      241 agagagttat ttctatcaat tactaaaacg gtggagtaag cttcccgccg atttttatcg
      301 aaaaatgcag atcatcaact atcaaccgct tgctgagaca catgcctaaa tagaggttgt
      361 gaaatcagtg ctttgagcta ataccgaagg tcagctgatt gaacattgtt tagaagagat
      421 tgtgacaact gcgatttgct ttgagtaata agaacgctgg acagaaaata gcttctcata
      481 ttttctagtc tagtgggctt attgccgaga agcagcagat ggaacatcgt ttagaagaga
      541 ttgtgacaac tgcgatttgt tttgagtaat aagaacgctg gacagaaaat agcttctcat
      601 attttctagt ctagtggact tattgccgag aagcagcaga tggaacatcg tttaaagaag
      661 agatcggtac aaaataaaaa tatctaaatt taagttttat ctaagtttcg ctttgtacct
      721 ttgattcaaa tcaaaggagg cgatcttgat gctgaaaaaa tggatcacac tattttttgc
      781 cactccccat tctagaatca acccgtatga tgtagataat gaattcacgt tgaaaaaaga
      841 tttctcaaat aaataaaaac catccgtaaa atcatcaata cgattttacg gatggttttt
      901 gataacttct ttgaggatag gctcttgcta tttaagaatg taagaaacat tattgttaca
      961 tagtatgaga gtttaatcct ctgttattta tgctatttaa atgcttgatt cacatcttcg
     1021 atcatcagtt tcattgattc taaaccgccc ccgcttaaat accagacatc tggttcaagg
     1081 gagatgatct gctgattttt accagcattc gtttgtgcta ccagttcatt ggcagaaata
     1141 tcatctttgc tgtcgtctcc accaatagct tttgttctgt ctactacaaa taagatatcc
     1201 ggattttttt ccagcacata ttcataagaa acactttgac cgtgagtgga tgcttcgatt
     1261 tgatcatctg cttgttcaaa gccaaataaa tcatggatga aaccgaatct agaaccagat
     1321 ccgtaggctg ataattgccc ttcattcaca agtgtgacta gtgctttttt gtcagtagct
     1381 tctgctttct ttttcgtctg atcgattgca ctagataatt cctctaattg agttttagct
     1441 tcttcttgct taccaaagat ctcccctaaa gcagtgacat tttgctggaa agattcccat
     1501 ggatttttcg catctaaaga aaggaaaatg gtcggagcaa tttgacttag atctttttga
     1561 aaatctcttt gtcggccaga aatgatgatt agatctggct gaagctgatt aattttttct
     1621 aagtctggtt cttttattcc acctgctgat tctacttttt tataggatga aagatactca
     1681 ggaaggctgc ttgtagctgc tccaactacc ttatctccaa caccaagagc atccatcgta
     1741 tctaaagaac cattgtcgaa tacgacgact ttttctggat tatttggcac ctcgatttgg
     1801 tctccattac tatctgttat cgtcaatgta gtttcagttt gtgtgctgct tttttttgtg
     1861 tctgcctgac tggtgttgtt gctatttcca catgctgcta aaccaaatga tgctagaatc
     1921 aagataccta ctactaattt tttcatctct tctctcctaa tgttagtttt agaaataaag
     1981 acagaatctg cggccttcaa tctcacaaat ccgtatgtcc atttcgtata attcatccaa
     2041 tacatctttt tttatcattt cttgggtcgt tccttgcgta aataactgtc catctttcat
     2101 tgcaacaatt tcgtctgcat aacttgcagc aaagttgata tcatgaagaa caataacaat
     2161 tgttttcccg atttcttgta ctaaattttt taaaatctgc atgatttgaa ccgcaaaatt
     2221 catatctaga ttgttcaaag gttcatccag taaaatataa tccgtgtctt gcgctagcac
     2281 cattgcaata tacgcacgtt ggagttgacc gcctgaaaga gtgtcaatcg attgatccgc
     2341 tatttcttct aacccaaggt gttccagtga ttcctgaacg atttcctcgt cttttgtcgt
     2401 caattttcct ttcgtataag gaaatcgtcc aaattctact aattctttca cggtgatttg
     2461 gacatttatt tcattcgatt gtcgtaggat cgataatttt ttggctaatt cattggattt
     2521 ccaagccttt acttcgttat ggtctaaata gatttcacct gtatcttttg gtatcaaacg
     2581 actgatcatc gacaacaccg ttgactttcc agcaccattt ggaccgataa aagcagtcaa
     2641 cttcttttca ggaatcgtta actgtacatc tttaaccact gattttccat ggtacagttt
     2701 attgaccttt tgtaaatcaa tcataggata ctcttccttt cgcgaatcaa ttttgtgaca
     2761 aaataaattc ctccgacaaa ttcaacgacg acactcactg ttgtattcca gccgaaaatc
     2821 tgctctactg cgagctgtcc tcctgtcaga aacagcatag ctaccagaaa gcttccgata
     2881 aatagctgac catgacgata agtagaaaag acacgataac tgatatttgc gacaataaac
     2941 cctaaaaaag taactggacc cacaagagcc gttgatgtcc ctgtcaaaag actgacacaa
     3001 cataaaatca aaaattcaaa acgatctgtg tctatcccga ggtttactgc ttgttcattt
     3061 ccgagatgga aaacgtctag cacatgtcga tatcgaaata acaggtacca gcagccgata
     3121 atgatcaaag aagcaggcaa aagatactga ctctggatat tgccaaagct tgcaaataat
     3181 ctgccttgaa gcaaatcata ttcattcgga tccatgatga cttgtaaaaa ggtactgaca
     3241 ctaccaaaaa gcgtacctaa aatcatccct gacatcagta ataaaaaaag attcgctctc
     3301 atttttttca aaagaaagaa aaataacaat agactcagcc cgaccatcag cccaacgttc
     3361 aataagaaaa aaactgtacc tgtctgctga agcatcgtca ctcctccgat aaagaagaaa
     3421 aggaccgttt ggacaaggac atataacgac tcaagtccca atactgaagg agtcaaaaac
     3481 tggttatgcg taattgtctg aaagctgatt gtggcactgc tgacagctag cgagacaagg
     3541 ataaatgcaa aaattttctt actcctcaat gcacatgcaa aatgccagtt tccatatgta
     3601 tggatcgtca agtacattcc tgtaacaaat aaaacaagaa caagcatacc aataaaaata
     3661 gatggctttt ttttaagcat atcgtcgtcc ccttatcaga agataaagga agatagcccc
     3721 accgatgatt ccaacgatca cactgactgg tatttcataa ggaaaaatca acacgcgagc
     3781 gacaagatcg caaaatacta agaacaaact acctgctaaa gcagttacgc caattgtttt
     3841 gtgcacttga tctccatata ttctagagac aagattcgga atgatcactc cgataaaagg
     3901 aatgcttcct accatgatca acatgactgc actagccaat gatactaagc caatgcctaa
     3961 aaactgcatc ttctggtaat ctattcccag atttactgca aacgattcgc ccagaccaat
     4021 gatcgtaaat cggtaagcaa atagccaaac caagagcaac aatggaacaa cagcatacaa
     4081 taactcataa tttcctcgta tcacagtcgc aaaattccct tgaagccatg aagtcatatt
     4141 ttgtaccaac tggaattgat aagcaaaaaa agtagcaact gaaccaatga tattccctaa
     4201 catgactcca atcaacggaa gcatcaattg atttttctgg aggaacaatc gcgtaaggga
     4261 taaaaaaagc aatgttcctg caaaagcaaa gcaaaatgct gtcaaggatc ggacaatcaa
     4321 ggatgcacca ggtaggaaaa tcatagccac taaaatacct aaacgcgcac tatccatcgt
     4381 gcccgcagtc gttggtgaaa caaatttatt ttgggtcaaa tgttgcatga tcaacccaca
     4441 aatactactg gtcgctccca caaggatcaa acaaatcgtt cgtggcaaac gagtcactag
     4501 caagatttgt aagctttgcc catctaactg gagcagttct gacaattgta cattcgatac
     4561 tccgatgaac aatgaaatac tacttaaaat aactaataat gctactagtc gtccgtagcg
     4621 catccaatcc acctttctaa ttgagaatga ttttcattct tattttaagt gaatcgttga
     4681 acaaaagcaa ccgctttttc atatgtttta ggtattattg ggaatatgct ataatcgtaa
     4741 gaaagacaaa gagaagaagg agggtatttt ttatggcaca agatgatgta aaaagccatt
     4801 tggataaggg actttatggt acacctttag tgaaccctga agaacagcat aaatatttag
     4861 ggacattcag ggaacgctgc tatcttagta tgactgttgc tgaaatgatc caaccaaaaa
     4921 acaaagaaaa ctttttaaaa gaactcaaaa aacatccaga tgcttccata ctcttgaatg
     4981 gggctctacc cctttctgtc caaaaccaat atatccagct agcaacgaaa aacaatagcc
     5041 gtttcactgt agtcaacgat tacgtcactg caaatcctga ggaattcgga ttgctcttaa
     5101 cagcaaaaga agcagtaaat gaacctgtga ttgatatcga aaaaaaatac ccagaagaaa
     5161 actcttcgcc agctgaaaaa gaaaaaccaa aaaagaaaaa aagcttctgg gatcgtttat
     5221 tttaaaacga aaaaacacat aaatttttac atgcaaaaaa agcttttgat cagattgaca
     5281 tcaaatagga caatctgtca aaagcttttt cttttaaaat acttcaggat ataacaattt
     5341 caatacattc atcgtcaatc ttctgccttt tcctgcaaaa ggataaacat gcatatgcat
     5401 ggctggattg aaatttgctt cgatgatccc atatgctttt ttatcagttg taggatcgat
     5461 ttctttatct ggtataatca aatcgatacc actaatctta gctcctagtg cttctaccgc
     5521 agaaacggcg atttttttgt aagcttcaga aaattcatca gtcatatcga tcgaatcacc
     5581 acctgtactg atatttgagt tctcccttag ataaacaatt tgattggctt ggggaactga
     5641 ttcgatcgtc aacccttgtt ctttcaacat caattgttct aattcaccta attggatcaa
     5701 ttctaaaggt gcacgatgat tcgttccacg caaagggtca ctattttttt cttcaacaag
     5761 ttctttcact gttcggatac tgtcaccaat aacattagca gggactcgca acatgatggc
     5821 ttggacttct ccgtcgatca caaagaaacg atattccgtt cccggcataa attcttctac
     5881 taaaaccgaa ctgtcttcac gaaaagcaat ggctaaacct gcttgataat cttccagact
     5941 agctccttcc ttgaaaatcg tgatacctaa cccgtaattg gttgatttag gtttgatcac
     6001 aaatgcctgc tcggcaaatc tcggataagc ttttaccgct tcttccatgg atgaaaattc
     6061 tgcgcctcct ggtacccgga acccagcttc ttttaagact tttttcgtta ctgttttgtt
     6121 ttccatgata agtgggacga tatagctgtc cttacttgtc atgtttgcat ttttcacata
     6181 ttctacatgg tcttggaatt gcagacgcaa aaattgatcc gactcatcta atactttgac
     6241 ttggacacct ttttggatgg catcgaacat aaatatctga gtagaaagtt ccatttcgcg
     6301 aaaaccggcc aactgatagg gacgttcatg gctttcttta taatattgat gtcccaatgc
     6361 cacagcaact tcttgctgac tcgatagctg tgcttcttga tagagtttcg ctgctaacgt
     6421 ttgttcagga tgatccattt gagtcaatgc ttcctctact aaataaagag aatccaacca
     6481 atccaattca accagcattt gcttcattcc ttcaagaatt tctctacctt cttgatgaaa
     6541 ttctgtttga tctgatggtt gttctagtgc tactttgtta tttcgagtcg taccttctgc
     6601 tacccattgg tcacagtctt ctttttcttc tgtccataac atgtacatca ggaataattg
     6661 taagaaccga acttgctctg gattgatacc caatcttgca taaggattca aatcgatatt
     6721 ccgcaattca atataacgaa cacctgttgt tgctaaatct gcgactttct ttccgcctcg
     6781 gaaacgaagc ggtgtataaa attctttctc ctccgaaagt ttcccttctt cgatcattcg
     6841 ctcgatatct gctaaatact gtttcataga agcataggaa actttcacat ttggatgatt
     6901 cgtatagccc agcgcactgt tgcgaatact gcgaacaggt tcttgcggat gtgattcatc
     6961 taaaaagtag ttcttctcac tcattggtga agcaccgaat aaataagtga tcatccagcg
     7021 atagcgcata aagtttctcg ctgttttcaa ataaagttct gttttgaatt cggaaaaatc
     7081 ttgaaattct tcctgatggc tgaacaatgt tcttagcaac tcatcgccaa attcaaaatt
     7141 gaaatgaata ccgcttacca tctgttttcg cttcccgtat ttttttgcta aataacgtcg
     7201 atacaagaca tcttcgaaat tttttaattt tgcaataatg atctcttcgt ctttttccgg
     7261 taaggcaggt ggcatactta atggccagat catctcttct ttcggtatag aacgagcagt
     7321 cacatcataa acagcagcga gatactgaaa taattcggga atagaatcgg taacaggggt
     7381 gatcatctct gtttgtgttt cgctaaaatc tgtttggata taggggtgat aggatcgatc
     7441 gccaaagatt gctggatgat cggtttttgc tagatttcct gcaagatcaa cccgctgtcc
     7501 ttcccgttct ataccaaatc gagcttggtc gataaaaggg cgcgcattga catgcaataa
     7561 taattgctta aaattcatca ttaagtgacc tccaattcat atgaatctaa ttataatcaa
     7621 aaagtctaag aaaaagaacg aaattgtttg agaaccgctt acaaataagt ataaaaatga
     7681 aggcatcaaa aacacagcta tgttaaaaac aatcattcaa acaaggagct gcaacttttg
     7741 tcacagctcc tgctttttag acatgggtta ttcttctttt agtaatcctt tgaacaatcc
     7801 aacgacaaaa tcatttggat caaacggctc caagtcatcg attccttcac caagaccgac
     7861 caatttcact ggtaaatgca attcattacg gatagcaagt acgataccgc cctttgctgt
     7921 accatctaat tttgtcaaaa caagaccggt cacatctgtt gtttctttga attgttttgc
     7981 ttgaaccatc gcgttttgac cagtggttgc atctaataca agaagtgttt cttggggtgc
     8041 ttctggtaat tcccgctgga ttactcgttt tattttttct aattcgttca tcaagttcac
     8101 tttattttgc agtcggcctg cagtatcaac tagtaaaaca tctgcctgct ctgtctttgc
     8161 tctttccaaa gcatcgaata cgacagctgc tggatcgcct cctgcattgc cacgaacaac
     8221 ttcaacacca gcgcgttcgc cccagacaac taactggtcg attgctccag cgcggaaggt
     8281 atctgctgct gccatcaata cttttttacc ttcgtttttg aactgatggg caagtttacc
     8341 aatacttgtt gttttcccta caccattaac accgacaaac aaaataactg ttagtccgtc
     8401 ttcttggata tttagttcat ttacttcatt gattccttcc gcttcataga gatccacaag
     8461 tttctcgatg atcgcatttt ggacttctgc cgattttttc gcatttctga gtttcacttc
     8521 ttgacgtaat gcttcggtga ttcgtatcgc tgtttcaaaa cctacatctg caccgataag
     8581 tgtttcttct aattcttcaa aaaagtcttc atcgactgtt cgaaaattgg caaacaactc
     8641 attcatccgt tcaccaaatg ttttacgcgt cttttctaaa cccttgtcgt acttctcctg
     8701 tacttcaact tcttctgttt tctcttcgcc agtaaaagca cgcttgattt tatcaaaaaa
     8761 tcccatttga ctccccctac tctaatacaa atttttggat cactttggct acgccatttt
     8821 cttcattgct ggctgtcacg aaatctgctg cttctttgac gaaaggcaca gcattctcca
     8881 tcgctacgcc tagtccagca tattggatca tcggtaaatc attttcctca tcaccgatcg
     8941 ccatgatttc ttcttgtttc aaaccaagat cttttgccaa taaagaaata ccataagctt
     9001 ttgtaacacc ttttggcata aattccaaaa gcatacttct tgttttgatg atctcataca
     9061 tttcatggaa ttctgctggt atttctttga ttttaggatc taattcttct tggtcataag
     9121 cgattactgc cttattaagc acaaattcat ctgtaatatc gcttatatcc gctggttgga
     9181 attgcagcag cttattcaac acattgtaca aggattgctt ccttggggca ctaggcagct
     9241 gtaaaaccac attgtcagac aaaacatcta gtggcagatc caattgctga gccagtttgt
     9301 ataagcgttg gatatctgat acttccatca ctttcttttc catgatttca ccagtatcat
     9361 ttttttgtac caaaccgccg ttgaaagtga tgctgtagtc accttcatct accagtccta
     9421 attcttgcag ataatgcgcc atcgcagcta aggggcgtcc tgtacataga acgatcttga
     9481 ccccttgctg ttttgcttgt gctaatgctt gtttattctc atctgaaatc agcttttgtt
     9541 catttaataa tgtaccgtcc aaatcgattg ctactaattt gatcattttc gttcctacac
     9601 tttcgtttcg ttgattttcg cctcttcatt gatatcttcc atccgtacag aaacgatttt
     9661 tgatacaccg gattcctgca tcgtcactcc gtacagtaca tctgctgcga ccatcgttcc
     9721 tttgcggtgt gtgactacga taaattgtgt gtcatcttgg aaatcgctca agtaacgacc
     9781 aaaacgtttc acgttggctt catctaacgc tgcttctacc tcatctaaga tacaaaacgg
     9841 aacaggacag acacggataa tggaaaagag caacgcgatg gctgtaagtg ctcgttctcc
     9901 tccagaaagc aaacttagac tttgcagttt tttgccagga ggttgtactt caatctcaat
     9961 acctgttttc agcaaatcag ttggttccgt cagtactaat tcagctttac cgccgccaaa
    10021 catatttgga aagacaattt tgaattcttg acggatagct tcaaatactt ctttgaatct
    10081 tgtccgcact tcatcatcca tctcatccat cgtttcaaac aattggtttt tcgctgctaa
    10141 cagatcatct cgctgtgttg ctaagaatgt atgtctttca ctgacttgtt cgtattgttc
    10201 gattgaattt aagtttactg gtcccagttt ttcaatctgt tcttttaagg atgaaacctt
    10261 tgttctactg tcttcgatat cggttgtttc ttgatagtct tgggaagctt tctcaaatgt
    10321 cagctgatat tctgactgca gataggtcaa atgattatct aaaatcattt cagctcggtc
    10381 tttttggact tctaatttgg attgttccga cagtcgtgct ttttgctcac gattacgctc
    10441 tgccaagact gcttcaagct tatcgatctc cttttgcctt ctatctcgct gctctttggc
    10501 ttttgccaac tcggctttta atgtttctcg ctgagcagat aattcgttaa tttgtttttc
    10561 caaactttct tctgtgactt catgatcaga aaagtctgct gtcaatgtcg ccaactgctt
    10621 ttctatcgct tcttgacgtt cggttgcctc tgctttttgg actcgggcac cgcggagctg
    10681 gatctgcaga tgattaaact gctctttcaa gacagcaaga tctgcttgtt cttgtgcttt
    10741 ttgactttgt acttgtgctc ttctggcttc catctggtcg ctttcttgac ttaacgattt
    10801 gatttcttcg tcgatttttt gtcgttgcga ttcaagatct gtttgtttgt ctttcaattc
    10861 gttcgtttgc ttttcgtaat cttcgataaa ctgctgcagt tcacgtgtct caaaagttga
    10921 gatttgcttt tctttttcaa aacgttcaag ttcatttatg atgttttgta gctgatttgt
    10981 cgcttcttgt tcttcaaaac gtaattgttc gcctcgtgta cgcaaaactt cttgttcttc
    11041 ggctaaacga gctgtttctt gttgcagctc ctgaactttt ttttcttgtg cttggagttg
    11101 tttatctgct tcttcaaatt cactagtcaa ctgtttcaat tcttgatttt gtacaaacaa
    11161 actgccttgg tttcctcgtt tattcgctcc acctgtcatg gatcctccgg cattcatcac
    11221 atcaccttct aaggaaacca ctcgatattg atagcggatc gtttgcgcga ttgcattggc
    11281 actagttaag tctttggcta aaaggatcgt tcctagcagg ttttgaacca cagtttggat
    11341 ttgatcggga taactgactt gttcactggc aatccctata aacccttcta cagctgctgc
    11401 ttgtgtcaat atatgagccg gtaattgtct tggtttgatt gtcgtcagtg gaaggaatgt
    11461 tgctcttcct cctctttgct gtttcaaata cgtgatggct tgccgagcat ctttttcatt
    11521 ttcaacgatg acatgctggg ctgccccacc taaagctgct tcgatagcta acgtgaaatc
    11581 tgcaggaaca tcgatcagtt cagccactgc tccgacaata cccgaaagct gctgtttatg
    11641 ctgcaagacc aatcgaaccc cttggtaaaa accaaaatag ttttcttgga tttcttgaag
    11701 gcttttctta cgagcacgta gctgctggac ttgattcatt aattgataca ttttaggctg
    11761 ttcttttgct aatgcttctt ggaacttttt cccgttttgt tggatcattt ctaacttctc
    11821 ttttgtttca gttaaagatg attttagctg cgcctgcttt tgtgtcaatt cttctttttg
    11881 taagatcaat cggtctactg atgcttctac ttccgattgc ttggctaacg tctgcttgct
    11941 ttttgctgtt tcttgtatat attgacgttc tagatacttc agttcgtttc ctacagctgc
    12001 ttgttcttgc atcaaatcaa catattgatc tcttaattct gccaataatt ctttcgttga
    12061 tttttggtat ttttctaatt cttgctgtgt ttttatgaga tttgcttcag ctttttggat
    12121 ctctgtttct ttttctgctg ctgctttcat caagctttct tgaagctttt caaaatgttt
    12181 aacctttttt tgtgcttctg ctaatgaagt ttgatattct tgcgagcttt tttgtgtatg
    12241 ctttgtccgc tcctgcaaaa catctttttg tccttctgtc tgcttcagtt tttccgaaag
    12301 atctaataat acttgctgat ttttttcgat cagacgatcg gcttgcgcat tttctttacg
    12361 ctgcttcgcc aaaatggatt cttgttcttg gatcgactca gatagtttgc ctaattctag
    12421 attgaatttc gcaagctgtg cttgtttgtt gtcccaatct ttttttgctg tcttgatttc
    12481 agcaaccatc aaagagacat cggtttgagt cagtgtttct tttagccgta aaaactcctt
    12541 tgctgcctca ctctgggcgg ccaaaggagt taactgttct tccaattcgt ggatgatgtc
    12601 ttggacgcgg ctgaggttgt cttctgtttc aaaaagcttc tgttctgctt tctttttccg
    12661 ctgcttgtat ttcaagacac cagctgcttc ttcaaagatc ccacgacggt cttccggttt
    12721 actactgaaa atcgcttcta cttttccttg agaaatgatt gagaacgatt cttttcctaa
    12781 tccagaatct aagaacaatt cttggatatc ttttaatctg cagctttgtt tattgatgaa
    12841 aaactcgctt tccccagtcc gtcgataacg gcgggttaca ctgatctcat tgtactccaa
    12901 tggcagataa tgatcactat tgtctaaaat cactgtgact tctgcaatat tcagctgctt
    12961 acgggtatcg gaaccggcaa agatgatatc cggcattttt ccgcctcgca gacttttagc
    13021 agattgttct cctaataccc agcggatagc ctctgtaata ttgctttttc cgctgccatt
    13081 tggtccaacc actgctgtga cgctattttc aaaatcaatg actgtcttgt ctgcaaatga
    13141 tttaaaaccg gctatttcaa ttcgtttcaa atacaaagct ttccctcact tacttttaca
    13201 gtgctgccaa tgcattttcc gccgcagctt gttctgctaa ttttttcgat ttcccttgtc
    13261 cagtgccaat caattgatca tctacataaa cttcgatcca aaatactcgt tcatgagctg
    13321 gaccttcttc attgatcaat cggtattcga ttgatacatc acctgatttt tgcaagactt
    13381 cttgtagttt tgttttatga tccatctcat gtgaaaaagc acctgctttg actttaggaa
    13441 aaatcacttt ttcgatgaat gcatgggtcg tgtcgaatcc ctggtcaaga tataacgcgc
    13501 ctaaaaaagc ttcgaatagg tcgcaaagca atgctggtcg cgtacgtcct cctgaattct
    13561 cttctccctt gcccaacaaa acatattgat caaagtgaca ttctttggca aatttcgaca
    13621 agctgtcttc gcgaacgatc gcagctcgtg tttttgtcaa ttttccttct ggcaaatgag
    13681 gatataaacg gaataaatac tgagaaacca tcagttctaa aacggcatct cctaaaaatt
    13741 caagtcgctc attatccgat aattgcagat tgcgatgctc attcacatag gatgaatggg
    13801 taaaagcttg ttcaagcaaa tttagatcgt gaaaaacgat gttatagttt tcttttagtt
    13861 cttttgtcag ctggttgtcc attttccaca ttccttttat ggatattttt tcgatacttc
    13921 cttattatac tggttttttg tcaaatttga aatggttcgg tcaaaaggta agtaaagctt
    13981 taataatcct agtgattgac gagaagttgt gaaaaattcg tttagtttcg agtatgaaga
    14041 aaaaattttg tgaaaatggc tttccatatt tttgcataaa tttgacttaa taccgagaaa
    14101 ctggactttg aacaccgttt attggagaag tcatgaaatc agcgccttga cctgaatatt
    14161 agagaaataa acagaaaaat atcaaaaaac cgaaaaagga catcgccctt tttcggtcat
    14221 ttttagtgat tatgaacgtg aatctgctgt aacagaaaca tttgcccatg ggtttggtac
    14281 atcataagac caatcccagt ctttgacacg gtcgttgact ggcaatactt cattacggaa
    14341 caacgttgga atcgcaaatg cttcttcaaa tgcatattgt tgccattttt gatatgcttc
    14401 tttttggtac tcttcatcaa atgctttgct tgaactgata tcgttgatca attttgtatt
    14461 ttcttctgat tcataacgag tgtagttgaa cgcagcattt ggtccccata gtccacttgg
    14521 atttggatca gttcctgtat tccaagcagc ttggtaaaca tcaatttcag gatcatcgtt
    14581 ttctaattta tcatagaaag aattgaattc gatcagacga cctgttgtgt attggacatc
    14641 caaaccaatt tctttccatt gttggatata gtaatctgcc aatggttgcg ctgtttcccc
    14701 accggacatc gaagcaaagt tgattgttag tttatttcca tctttgtctt ctcttaaacc
    14761 atcgtcatcc acatctttgt agccagcatc atctaatagt tttttcgctt tgtctagatc
    14821 ttgtgtgaat cctttcatct catcatcatg gtatgactgg aatactggag gaatcaatga
    14881 agtcgcattg cttctcaaac ctgcatagaa acgttcacct actgcattat tatctaatgc
    14941 atagcccatt gcttgacgta atgatttatc agccattttt gaatctggat tgtattccac
    15001 actctttgta tctgcattcc atttacctaa cttgaatcct agataagtat aagaagtttg
    15061 ttcacggcct aagtttgtat agccagcaat ttctttatag gtatcgtaag tatctgttgg
    15121 catttggaaa atcatatcat ataatttaga gttcaatgct tcagttgcac ttgccatcgg
    15181 tacagatttc atcgtgatct ttttcaattt aggcgtacca ttccagtagt gctcgtttgg
    15241 caagtattct actgattctc ctgcaacgat attactcatg taataagggc caaaagtcac
    15301 tggattttta cgtacttgat cgcttgattc catatctttg attgctacac cttctaacgt
    15361 atgttttggt gctgcatatg accagatacc accacccgat tgaagcatgc ttgggtttac
    15421 ttctttatat tcgatttcaa ctgttttgtc gtctattttt ttgattccgg agattgtgtc
    15481 ggcacttcca tcatgatatt cttccatacc tacgatgtta cggaaagtgt cgtcataacg
    15541 gataccagtg taatctttat tcccaatgat ttcgtaaggg aaaatcacat cgtccgccgt
    15601 tacatcttca ccatctgacc atttgacgtt gtcttttact ttgattgtcg cttttttcgt
    15661 atcttcatct agatcaagcg ttgcagctcc gtcgtctgtg attctgaaat cgtcatcgtt
    15721 tgcaaatagc ggttcatgag aaggagccat aaaagctgca tcatatgaat cttggtaaaa
    15781 ctcccactgg aacaatcctt taaactgact gtccgttgcg accgcagctt ctaattcgcc
    15841 gtcttcgatt gcatcatcat tattttttgt tgcaattgga aaagttaatt cctctttgtt
    15901 attgccgttg cctccagagt tgtttccaga ggtatctgta cctccgcccc cgcatgctgc
    15961 aagaacgaca gcagacatca gcgctgcaaa tccaaaaatc gttttcttca ttcctatttc
    16021 ccccttcgct tctatactag cctaaccgtt ggcgcgcatc tgcagaacgc ttgaatgctt
    16081 gtccaacata attgatactc aacatcaaaa ctaaaataag gattgatgcg ggtaaccaga
    16141 tccattgccg attcactaag acttctccgt tacttgcata accgatcaat gtacctaaac
    16201 taggtacatt tgccggcaag ccgaaaccta agaaagtcaa agttgtctcg ataccgatgt
    16261 ttgccgcaaa gttcatcgtc aaattagtaa tgattaatga acttagattc ggcatgattt
    16321 cgcggaacat gattttaaaa tcattggttc ccattgtttt tgaggcactg acataatctc
    16381 ttcttgcttc agataatgtc ttgcttcgga ataatcgggc tttagctacc caataaaatg
    16441 cactcatgat gaatataaag gaccagacat tgtatctgga aataatcgat acgaagacga
    16501 taatgatcag catgatcggt agaatcatga taaaatcaac gatccgcatc aagatgttgt
    16561 caatgatccc tccgtagtag ccagagatga tccctaatcc aaccccgatg atcgaagtaa
    16621 ggatcgtgat cgcaaatccg atcgtaacag agtttctagc acctatgatc aattgtccta
    16681 atacatcacg tccgccttca tctgctccca gaatgaactt ttgtccttgt gtcgaaactg
    16741 ttcctggtgc agcgtatttg tcgaagatac taacatacat cactttgtct tgatcggtca
    16801 gcatcgcacc gataaagatc actaataata aaaccactag caaacctaat gaaaaaatag
    16861 caattttatc ttttttaaat tctcgagcaa tcattcgtat ccccatagga ggaatacttt
    16921 cttgaactgc tgtattgttt ttttcactca tcgatccatt ctcctttctg attggtttta
    16981 ttgaatccga atacgcgggt caacgatact catgatgata tctgaaagta atgtccccac
    17041 tagcgtcgcg attcctaaaa tcaatactaa ggatgtgatc acggtatagt cccgttggcc
    17101 gatggaatca ataaatagtt ttccgactcc cggataacca aaaatctttt cgataactac
    17161 agaaccagca atcaacgaag tgatttcata ccccatttga gaagccatcg gcaacgctgc
    17221 atttcggaaa atatgtcgac tgaatacttt attcgtcggt acccctttgg aacgggctgt
    17281 tctgacaaaa tcgagcgatt tcgaatcgat tacttcactt cgcagatatt ggattgtgac
    17341 ggcagtcgcc aaaaatgctt gggtaattga tggtaacaga atatatttca aacggtctat
    17401 ccattgagtg ataaatccac cttgcaaacc tagtgtttgt gatccacttg ttgggaacca
    17461 gcctaaacga taaccaaaaa caaatagcat gatcaagccg aagataaata aaggaacagc
    17521 aaaactaaag aagttgtaga ttacgacaat cttatccaag attgaatttt gataccggcc
    17581 agcaagcatc cccagaggca cagcaatcaa ataagtaagg acaactgtca agatcgatag
    17641 atataaagtg ttgatgatcc gttcgccgat caagtcagca acagagcgtt tgtataagaa
    17701 actttgacca aaatcgcctt gcaaagcatt tccgatccaa cggaagtact ggacatacca
    17761 tgggtcattc aagccagctg cttgtcgcat ctgttcgatc actgccggat cctgatttgg
    17821 attgatcaaa ccggtaaatg gatcccccgg catcgcttgg gctaataaga agatcagaac
    17881 acttaaaata ataacttgcg ggatcatcaa taaaacacgt cgcagaatcg ttttccacat
    17941 tagcttgcac ctccatcttt caacgccact ttgtgtgtag ttgtgatcgt acgcaaatcg
    18001 tagactcgac cagtcgcatc atagtattct ttttggtttt ggatatactc ttgttctact
    18061 cttcgtcgat tttctttgtg gctttcccta ttttctacat cgatttgcgg aatagctgaa
    18121 agcagacgtt tcgtatagat atgtcgcggg tcattataaa tgtcttctct tgtaccgatt
    18181 tctacaaatc gacctttgta cataattgcg atattgtcgc acatgtgttt tactacacct
    18241 aaatcatgtg aaatgaataa atagctgagg ccgtattctt gctggattcg tttcatgaaa
    18301 ttcaatactt gcgcttgaac agatagatcc agagcagata caggttcatc ggctacgatc
    18361 agttttggat tggtagcaac agcacgggct actcctagac gttgacgttg tccgcctgaa
    18421 aattcatgag gatatttata tagcgcatct tccggcatcc caacgatatc tagcagtcct
    18481 tttacctttt tcttttcttc ttgatcgctt aaccgttcaa aatttcggat gggttcagca
    18541 atgatatcca acactcgctt tttaggattc aaactggaca ttgaatcttg gaagatcatc
    18601 tgcacatcct tgttataacc catttttttg cggttgcttc gcttcgttac atcttttcct
    18661 tcatacatga tttgtccggc tgttaccttt tctaatccca caacggcttt tcccgtagta
    18721 gattttccag agccagactc tcccactaaa ccatatgtct ttcctttttc gataataaaa
    18781 tccacaccat ctacagcata tacatgatcc gttactcgat tgaagaatcc actgcgaata
    18841 gggtaatgaa ctttcaggtc tttgattgtg attaattctg tcatctacac ctctcctttt
    18901 tcgtctctaa agtggaaatg cttatagcag gagcaacgta ccaagtggtt cggtgcgact
    18961 tcatgaagga taggctcttc ttcatgatcg cttgctggta tccatggaat acgtggtgca
    19021 aaccgacaac ctttacgagg catgtttttc agagaaggaa cgacaccttc gatcacatgg
    19081 agttctgcat catgatttcc ttcttgtggt atagagttca gtaaagagcg tgtataagga
    19141 tgcttaggat gatcaaacag ttcttttaca cctgctactt caacgaactg tccgccatac
    19201 atcactgcca ctcgatcagc catttctgct acgactccta agtcatgagt gatcaagatg
    19261 atccctgttt ttgtttcttc ctgaaggtct ttcagtaaat caagaatctg tgcttggatg
    19321 gttacatcta gggctgtcgt cggttcatct gcaatgacga ttggaggttt gcaggcgatt
    19381 gcgattgcga tgatgacacg ttgtctcata ccgcctgata gttcatgggg atattggcgt
    19441 ccaactctcg caggattagg aatccctact tgttctagca attctagtgc acgagcttga
    19501 cgctgttctg cattcatctt tgtgtgatag gaaagtcctt ctttgatctg gtcttcgatt
    19561 ctcatgagag gatttaatgc agacaacgga tcttggaaaa tcattccgat atcatttccg
    19621 cggattttat tatagagcgt ttcgttcagg tcgattaaat tcagatcgtt atataaaatc
    19681 tcacctgtaa tttttgtgtt attaggatca agtaatccca taatcgtagt agccaaggta
    19741 cttttcccgc acccagattc cccaacgatt gctaaaatct cgtttttgtc taaagtaaat
    19801 gaaacgtcat ctactgcatc gtaatagtca tctttgagtc ggaatcctgt atgtaggttc
    19861 tgaacttcta ataattggtt atcggacaat cgaatacccc tctctgcttc ctaaatggaa
    19921 gtttttctta ataaaaaagg acattacaca gaataaagcg ttttataaca aaggttattc
    19981 ttatctttgt gtaatgaa
//
LOCUS       DAYYHX010000037        19757 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000037 DAYYHX010000000
VERSION     DAYYHX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.037"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..1309
                     /locus_tag="ACHA6Y_000963"
     CDS             50..1309
                     /locus_tag="ACHA6Y_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM8987076.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(1398..2345)
                     /locus_tag="ACHA6Y_000964"
     CDS             complement(1398..2345)
                     /locus_tag="ACHA6Y_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8987077.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            2739..4031
                     /gene="hisS"
                     /locus_tag="ACHA6Y_000965"
     CDS             2739..4031
                     /gene="hisS"
                     /locus_tag="ACHA6Y_000965"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM8987078.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4046..5815
                     /gene="aspS"
                     /locus_tag="ACHA6Y_000966"
     CDS             4046..5815
                     /gene="aspS"
                     /locus_tag="ACHA6Y_000966"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM8987079.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            5924..6799
                     /locus_tag="ACHA6Y_000967"
     CDS             5924..6799
                     /locus_tag="ACHA6Y_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8987080.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            7065..8948
                     /locus_tag="ACHA6Y_000968"
     CDS             7065..8948
                     /locus_tag="ACHA6Y_000968"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8987081.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(9023..9307)
                     /locus_tag="ACHA6Y_000969"
     CDS             complement(9023..9307)
                     /locus_tag="ACHA6Y_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8987082.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(9297..10031)
                     /locus_tag="ACHA6Y_000970"
     CDS             complement(9297..10031)
                     /locus_tag="ACHA6Y_000970"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM8987083.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            10160..10783
                     /locus_tag="ACHA6Y_000971"
     CDS             10160..10783
                     /locus_tag="ACHA6Y_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM8987084.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            10975..12096
                     /locus_tag="ACHA6Y_000972"
     CDS             10975..12096
                     /locus_tag="ACHA6Y_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8987085.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            12103..15231
                     /locus_tag="ACHA6Y_000973"
     CDS             12103..15231
                     /locus_tag="ACHA6Y_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8987086.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            15250..18444
                     /locus_tag="ACHA6Y_000974"
     CDS             15250..18444
                     /locus_tag="ACHA6Y_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM8987087.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(18590..19297)
                     /locus_tag="ACHA6Y_000975"
     CDS             complement(18590..19297)
                     /locus_tag="ACHA6Y_000975"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8987088.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         6615 a         3476 c         4159 g         5507 t
ORIGIN      
        1 aagttaagct taaattatac aagttatttt ttataggagg ttgctgaaga tgaaagaagt
       61 accagtagta gatatcaaaa tcacagattt aaaaaaattg tatcaaaaag gatacaacat
      121 gttggaggag ttacgccatg aagcggacgc tcccgtcgtc aaagcaaaga ttttcaataa
      181 agaagctatc acgatctacg gatcatcagc agcaaaagta ttttatgatc caaggaattt
      241 caaacgaaaa ggtgctatgc caaaattagt attgaagaca ctttttggtc aaggtggagt
      301 acagactttg gatggagctg ctcatcacca tcggaaaaat atttttatgg atctaatgac
      361 accggaaaga atggaagatt atcatcgtat cttagataag aatctgacac aagcacttga
      421 agcacagcat ggtcaatttg aactatttga tttaagtaag atggttttct ttacatctat
      481 ttgcgagtgg gcaggtatca atttatcagc gatttctaaa gatgaggtcg aaaaattagc
      541 agaatatcaa atctctatga tcagcggaac gtttacttct ccgattgatc acataaaagg
      601 tgtcgaaaat cgcaaaaaat cagaaaaatg ggcgcaaggc ttgattgaag aagctcgaca
      661 aaatcctgtg gctggaaaag aaaatgtggc attatatgct tttgccaatg ccacagattt
      721 agatggtcag cttttacctc tggaagtggc agctgttgaa ttattgaaca tcatccgtcc
      781 aacggttgct ctaactgtgt gggcggcatt gatgggacat gcacttttca gtagaccaga
      841 tttgtatcag caattaaaaa atgacttttc gacgttacaa gatccgttta ttcaagaaat
      901 gagacgctat tatcctttct tcccaatgct acctgcgatt tcgctgaaag aagtagaagt
      961 agatggttat cgtataccag aaggcagttg ggttatttta gatttatatg gaacagacca
     1021 tgatgaacga acagttgaag cgccagattc ctttatgatc aaacggtatg tagggaaagc
     1081 aaaagatatt tcctataaag aagaatatga gatgattgca caaggcgggg gaaattttcg
     1141 acaaatgcat cgctgtgcag gagaatggat cactcttcat agtctacgtg tgttttccga
     1201 tcagctagta aataagtttg agttctctgt acctgaacaa gattggacga ttccattcaa
     1261 tcagtttccg acttatccga atagccgagc gctactttac aagaattaga ttagcataaa
     1321 cttatcgaaa aaaaggaaga tcaaacagtg ctgatcttcc ttttttcata ttactataaa
     1381 ttgataaggt caattgatca aaatccacga aatgcttcat caatggtttg ataatcttca
     1441 ttggataaat gcaaatctaa agcttggaca ttgtcagata cttgctctgg acgcttagct
     1501 ccaggtataa ctacagacac tcgtggattc ttcatgtacc aagctaaaac aagttgcgca
     1561 ggagtggctt ggtatctttt agcaatgggg cgaagtacat cgacagcggt taaaatttct
     1621 ctgaaacgtt ctccttggaa atctggattt ctttttcgag gatctccttc accaaattgt
     1681 ttttcctcac caagttcata ttttcctgtc agcaaaccgg aagcaagagg aaaataggga
     1741 acaaaactga ttttgttttt ttcaagataa gggaataatt ctttttcagc ctgccgatgg
     1801 atcaaactgt atttatcttc tactacatcg acatatccat ctgcattcgc ttctttcaat
     1861 tgttctaacg tgaagtttga cacaccgact gctcggattt ttcctgcttc ttttaattcg
     1921 tgcaatgtag caactgattc attttttggt gtgctttcat ccggaaagtg gatataaaaa
     1981 atgtcgatgt aatcagtttg cagacgtttt agtgcatctt ccaccgattg tttgagaaac
     2041 tcaggagaat tatcaaatgt tcgcccttta tttggtacat gagcagcttt tgtagcgatg
     2101 acgacacgtg agcgatcata ttctttttcc ttcagtacct cgccaatcag ttcttcggaa
     2161 cggccgtttc cgtaagcata agcggtatcg atcaaattga tcccgctatt caaagctgta
     2221 cgaactactt gttttcctgt ctcatcttcc aaaccgtcaa aaagattatg tcctccaacg
     2281 gcatttgctc ctaatccaag ctgttctgcg tagacttgtg aatgtccgat tcttatttct
     2341 gccatagctg attcctcctt tttgtctagt ctaaaacaaa ataccaaaaa gctgaactaa
     2401 aacgtttttg tgaggggaaa tagtcagata tctcttgact ttgagactgg tttttagtag
     2461 gttataagta atatcgaatg tcaatgaaag agcgagtaag tttttgaaac tttacaagag
     2521 aggatcatcg aggctgaaaa tgatctaaag tttcattagc ctaaagacac tctggagatg
     2581 gacgagtgaa aagccgtccc gcgtacgagc gttattcgta agaggaaaga gaaatctttc
     2641 aaaattaggt ggtaccgtgt atgaatttca ttcacccttg ttcatgatga acaagggtga
     2701 tttttttatt tacatagcta agaaagggag agaaaactat gagtttccaa agaccaaaag
     2761 gaacaaatga tattttgcca ggcgaatcag aaaaatggca gtttgtcgaa gaaacagcac
     2821 gtttattatt cagagattat cagtataatg aaatgcgtac accgattttt gaacactatg
     2881 aagtgatatc ccgaagtgta ggggatacaa cggatatcgt ttcaaaagag atgtatgatt
     2941 tttatgacaa aggggaacgc catgtgacgc ttcgaccaga aggtacagca ccaatcgttc
     3001 gttcttttgt tgagcataaa ttgttcggcc cagagtatgc gaaaccgttc aaaacttttt
     3061 atatgggacc aatgtttcgc tacgaacgcc ctcaaaaagg acggttacgt caattccatc
     3121 aaatcggagt agaagcattt ggatcagaaa accctgcaac tgatgtggag agcatggtga
     3181 tggcacttga tttcttcaaa cagttgggga tcacgcaaat ccggctagtc atcaattcat
     3241 taggtgataa agaaacgcgt caagcctatc gtcaagcatt gatcgactat cttttacctt
     3301 ttgaatcaga gctgagtgaa gactctaaac gccgccttca tgaaaatcca ttacgcgtat
     3361 tggacagtaa agacaaacga gatcaaaaat ttgtggcaga agctccatct atcttagatt
     3421 atctaagcga gcctgctcaa aaacattttg ataccgttgt ttccatgctg gatgcattgg
     3481 ctgttcctta tgaaatcgac agtaatatgg ttcgcggact agattattac acacatacga
     3541 tttttgaaat catgagtgac gcaccaaaaa tgggggcaca agcaacgatt tgcgcaggcg
     3601 gacgttatga caatctagtg gaagaactag gtggtccttc aacacctggc ttcggatttg
     3661 caatgggtat cgagcgatta ctcttgacta tggaggcaga ggaagtagtt atccctgcat
     3721 ttaatgagtt ggatgcttac gtggtcgcac tcggagacga aacaaatatc gaagcattga
     3781 aagtggtcca agcgattcga aactttggtt tttcagctga ccgcgacttc atgaaccgta
     3841 aagcaaaagc gcaatttaag acagcagata aagcgaatgc caaattagta ttggtgatcg
     3901 gtgaagatga gcttgctagt caaacgatta atgtcaaagc aatggcaagc cgaaaagaaa
     3961 aaagttttcc attagaagag atatatgaac attttgatga agtctatgat gaaatgactt
     4021 tattcgagta ggaggaaaaa aataaatggc acaaagaacg atctattgcg gattagtttc
     4081 cgaacaacaa ttagaacaaa cagttacatt aaaaggatgg gtacaaaaaa gacgagacct
     4141 tgggggagta atctttatcg acctacgtga tcgtgaagga atcgttcaaa tcgtctttaa
     4201 tcctgaaatc aacaaagaag catgggaaat cgcggataaa tgtcgcagtg aatacgtgat
     4261 cgaagtgact ggggaagtta gaagacgcgg agaaaatgca gtaaatccta aaatggccac
     4321 tggtgaattc gaagtgattg caagtgaaat cgtgattttg aatcaagcaa aaaccccgcc
     4381 atttcaaatt gaagatgatc aagcaatcag tgacgaaata cgtttgaaat atcgttattt
     4441 ggatctgcgt cgtccaaaaa tgactaaaaa catcaaatta agaaatgcga caacaaaagc
     4501 gattcgtcat tatttagatg ataatgactt tctcgatatc gaaacacctt acctagggaa
     4561 atctacacca gaaggcgcaa gagactacct agttccttca cgtgtccatg ctggacattt
     4621 ctatgcgttg ccgcaatctc ctcaattgtt taaacaattg ctgatgaatg ctggattcga
     4681 ccgttattat caaatcgttc gttgtttccg cgatgaagat ttacgtggag accgtcagcc
     4741 tgaatttacg caagtagata ttgaaacaac gttcttaaca gcagaagaaa tccaaactta
     4801 cacagaggga ttgattgcga aggtaatgaa agaagttcgc ggtatcgaag tcacattgcc
     4861 atttcctcgt atgacctatg atgaagcaat ggctcgctac ggcagcgaca aaccggatac
     4921 acgttttgat atggaattga tcgatctaag cgatacagtc aaagaagtag aattcaaagt
     4981 cttccaaatg gctcttgaaa acggcggtgt cgtgaaagca ttgaatgcta aaggcgctgc
     5041 agatcgctac tcacgaaaag acatggatca gttaggtcaa tatgtaggac aattcggagc
     5101 taaggggctt gcatggctga aagtagaaga agacggattg aaaggtccaa ttgctaaatt
     5161 catgggagaa gcaacagaag caatcattaa agcaacagat gcaaaaccag gtgacttgct
     5221 gatgtttggc gcagataaaa gtgaaatcgt cgctgctgca ttaggtgcta tccggacacg
     5281 tttaggaaaa gaattaggac taattgatga atcgaaattc aatttcttat gggtaactga
     5341 ttggccacaa tttgaatttt cagaagaaga aggtcgatat gtctctgctc accatccatt
     5401 cacgatgcca aaagaagaag atatcccgtt attggctgaa gaccctgcaa aagtctatgc
     5461 agaagcttat gatatcgtct tgaacggtta tgaactaggc ggaggttcta tccgtattca
     5521 tactcgtgag cttcaagaaa aagtactaga aactctaggc tttacaaaag aacaaatgga
     5581 agaacaattt ggtttcttgt taacagcttt agattacgga ttccctccac atggcggtat
     5641 tgcattagga ttggaccgtt ttgcaatgtt attagccgga gaagataata ttcgtgaagt
     5701 cattgccttt cctaaaaacg gaaaagcagc agatgtgatg agtgatgcac caagtatggt
     5761 ttcgccttta caattgttcg aattgaatat tgatgtaacg aatatagaag aatagccgat
     5821 aaaaaggtaa aaaggatttg tcgaatacgc ttcttttgac aaccttgatt taattgaaaa
     5881 acactttata atgaagccat aacatataaa gcggggcttt tccatgaaaa aaacattcga
     5941 aaattgggct tatcatgatt acacgaccaa gctttcggta tctatcgttt atgcgttgat
     6001 cgcatcagtc gcattgaatt tcttttatca gccaggacat atttattcaa gtggtattac
     6061 tggtctagct cagattttga cgactttgtc aaaatcactg ataggattcg aagtaccggt
     6121 atcaacaact ttgtatttac tgaatatacc tttgtttgta ttggcgtggt taaaaatcgg
     6181 gaagaagttt acaattttca cttttatcac agtcactttg acctcccttt gtatccatat
     6241 tttaccggaa acagtcctat ctaccgatcc aatcatctgt gcgttgtttg ggggggctgt
     6301 gatgggacta ggtattgggc tagcgttgag aaacggccta tcatcgggcg gtctagatat
     6361 cgtgagtatc acgattcgta aaaaaacagg aaagtcgatt ggttctattt cgatctactt
     6421 caatgcattg atcgtttttg ttgccggaat tctatttggc tggcaatata tgttttacag
     6481 tacgttgtct attttcgtca gtggaaaagt aactgatgtc gtttatacta aacaaaagaa
     6541 gatgcaggta atgatcgtaa cgaaacatcc agaaaaagta gtaaatgaaa tccaaaactg
     6601 tatgcgtaga gggatcacga tcatcaatga agcagaaggt gcttataagc atgataagca
     6661 gacagtattg atgacgatcg ttacacgata cgaactgcct ctacttagag aagcaatgaa
     6721 agcttctgat ccacaagctt ttgtaagtat tgcagaaaac gtccagatac ttggtcgttt
     6781 ctatgaggat ggcatttaga tcgagtttga caaaaattca tgaaatactg ctgtagacag
     6841 acgttcgttt gtctgcagtt tttttctacc atagtagaat cagcaaagaa aaactgcttt
     6901 tttatttgaa attttcttaa agttgaaaaa aagcatgttt atttttttaa atttatggga
     6961 tgatagaaaa aaatagtaga gaaaggacac gattctactt gctattttca cttaataccc
     7021 gtatattacg aatgaaaaaa gtttatgaaa agttggaata ttgaatggaa aaacaaaaaa
     7081 ggttaaaaaa cagccgctac atcactgggt ttgatgggct ccgttcatta gctgtggtgg
     7141 gtgttatctt ctatcacctt ctgccaacta gtatgaaagg cggctatttg ggcgttccga
     7201 tttttttcgt cgtatctggt tatttgatca cggatctgct gcggcaggaa tgggaacaaa
     7261 atggaaaaat caatatttgg caattttatg tcagaagaat gaagagactc tatccggcac
     7321 ttgtttttat gcttgtgaca gcttcggctt atatcacttt gtttcagcga gggctgttaa
     7381 acaatcttcg aggagttgtg gtcagcagct tactgtatgt caacaactgg tggcaaatca
     7441 ataacggttt atcttatttc gatcgatttg ctaatgagtc accgtttacg catatctggt
     7501 ctttagcagt agaaggtcaa aactatttgg tttggccgat cttgtttgta ttgttgatgg
     7561 tatttgttcg taaaagaaaa tggatctttt atactgtgtt aggtacatcc ttcctctccg
     7621 ccatcttgat gatgatcttg tatacaccag gaggcgatcc aacccgtgtt tattacggta
     7681 ccgacactcg tctgttttct atatggttgg gaagtgcgct tgcgtttgtt tggccaagta
     7741 cgcgtttaaa gaaaaacatc ccgaaacaag cgaaaaatat tctgaatatc gctggaggaa
     7801 tctcgctttt ggcactgatt ctttgcttct tctttttaga tgatcattac aatttcatct
     7861 attacggagg aatgctccta gtgagtctgt tatgcgtagt gctagttgca gtgacagcac
     7921 accccggagc aagtttgaat cgatggctga caaatccagt atttacttgg attggcaaac
     7981 gaagctatgg catttatctg tatcagtttc cagttatgat tttttatgaa gcaaaggtga
     8041 ctgatattgc cgatcatgtt ttgttgcata ctttagtgga aatcacattg attctaatca
     8101 tcagtgaact ttcttatcga tatgtggagc gtccgctagc acgttttgat tatggtcgta
     8161 cattccaagt gatgaaagga tggttcagca aaccgatcat cagtaagagc aaaccatggg
     8221 ttgttccagg cacattactt gtagcaattg cactcgttgg tttcgttacg gcaccgaaaa
     8281 acgctgtaac agccgatcaa aaacaactgc aggcaaaaat cgctgaaagt aaaaaattag
     8341 cagaagaaac cagaaacaat tcagaaaaca ccactagttc ggttgaccaa gcgatccttg
     8401 ataagtacga gttgtccaag gcacaaggga ataaggcaca aaaattagaa ctaacggcat
     8461 ttggtgattc agttatgttg gatgctgcaa cggatctaca agaactttat ccaaaagtag
     8521 tggtagatgg agatgtcgga cgacaattat atgccagtgt accgtacatt gaaaaattaa
     8581 aggaacaaga tttgttgaaa gacactgttc tgatcggact aggaacgaat ggctcattct
     8641 cggaagctca gtttgatagt gtgatggatg caatcggtga tcgaaaagta tactgggtca
     8701 atgtccgagt accgacgaag cgctggcaaa atgacgtgaa tgcgatgctt gaacagatga
     8761 cgaaaaaata cgacaatatg acgctgattg attggtacag ctacagtaat gaccatagtg
     8821 actggtttta tgatgatcaa gtccatccaa atcctgaagg gatgacacat tacattcact
     8881 tagtcagcga agccttgcta ggaggaagtt caagtacgaa cagcagtgag acaaacgtga
     8941 cagaatgata gctacaaaaa acttcaggca aaaacatttg tttttgcctg aagttttttt
     9001 atatttaccg tgtttttaac attcatatag attcaggaag caaaacggtt gagtgtgtct
     9061 gtaaataacg ttctttttgt tttcgggtga gttttttgaa tgcagcttct gcttttgttg
     9121 cttcgcttct tgaagaaaat acttctgcat ggatcatctt aacaggtctt cttttttgaa
     9181 gacgagtata tttagcacca gtaccattgt tatgttcctc taatcgtcta gaaagatcgg
     9241 ttgtatatcc accataaaat gtctgatctt tacagaataa tacgtaaaaa taatgttcat
     9301 ttgccataga gcatctcacg aatctctgca gtgtattctc catccatatt gtaagtgatc
     9361 aatggtggag cgactttgaa tccgtctaat ttgccttctt tgattccttc aactaacaaa
     9421 atatttgcct cttttccagg ttttgggtaa acaaattgga tccttttagg tgcaattcga
     9481 tagtcttcca tgacctgaat gatttctaag aaccgatcag gacgatggac catagtaaag
     9541 cgtccatttg tttttagtag cttacttgaa atatcgatca cttcttctaa cgtcgtatga
     9601 atctcatgac gggcgattgc caggtgttta ttagggttcg ttttatttgt tggcagccct
     9661 ttgaaataag gtggattaca gacgactaag tcacaagaat tatgtcgaac gacagataag
     9721 ctgtctttca gatcgatggt gtggacagtc atttgttcat ttaaatggtt aagcgcgata
     9781 ctacgtcttg ccatgtctgc tagacgaggt tgaagttcga tagcgtcgat agccgcctgg
     9841 gttcgtttac ttaaaaaaag tccaactgcg ccatttcctg cacaaaggtc aacaatcttc
     9901 ccttttttag gtacacggga aaagtgagca agcagcacag catctaaaga aaaagaaaac
     9961 actttggaac tttggataat cttgatatct tctgcgtata attggtcgat tcgttcgtct
    10021 tcgtgaagca tggattccct ccaaaatgaa aaaaatagtc gtctgtatta tactattttt
    10081 attaaaaaga accaaggtgg cttgtcaaac ctctattttt tatgcatact atgaccataa
    10141 gaagggaaag gaagagacta tgttttatcg atttatgcgc ggggtagttc gagtactatt
    10201 agccttagtt aatggaaatg cccgctatga gaataaagaa gttttaccgc aagatgagaa
    10261 ttacatacta gttgctcccc atcgtacttg gtgggaccca ttatatctgg cggtagctgc
    10321 agcacccaag gaattcagct ttatggcaaa agaagaactg ttcaaaaatc cagttctgcg
    10381 atttatatta actcgatcca atgcttttcc agttaaaaga gaaaatccag gaccaagcgt
    10441 cattaaaaca cctgtaaaga ttttgaaatc gacaaatttg agtttgatca tgtttcccag
    10501 tgggacaaga catgctacag aacttaaagg cggaatggca ttgatttcca gaatgtctaa
    10561 agcgaaaatc gtgccagctg tatatcaagg tccgttgaca cttggggatc tgttcaagcg
    10621 tcagagagtg acagtgcgtt tcggcgaacc aatcgactta tcagatatca agaaaacgga
    10681 taaagaaggt atggcggaga tcgaacgacg aacacaagca gcttttgaca aattggacaa
    10741 agaagtgaac cctgatttca aatatgaagt caaaaaaaaa taaaatgata agtttatgcg
    10801 tgggcggtct tttgaatcta ctgtcctgtt cagttcagac tttttcaatg aaaacgaggg
    10861 agagacgtca agtctgccct ctttttgcgt tccaatatgt aaaatagaag acaggtgtca
    10921 tgtaatggat caaagtgtta taataattag aaggacattt tttgaggtga ttgaatgcgt
    10981 tttttacata cagccgattg gcatatcgga aaaaaactac aaggttatga cttgattgaa
    11041 gaccagcgtc atgtactatc ttctatacta gatatcgcga aagaagaaaa agtagaggca
    11101 atcgtgattg ccggagactt atatgatagg agtgtcccag ctgttgatgc cgtagaatta
    11161 tacaatgaat tgatggtcga ttggaattta aaggaaaaga tcccgatttt tgctatatca
    11221 ggaaatcacg atagcagtac gagactttca gcgggtacgc catggttcag ttattcgaac
    11281 tattttttgc gaacacagct agcagaagca tttgaaccag tcgagtttaa aaacgtacaa
    11341 tttttctttc ttccttactt tgaaccaatt gcagcgagat tatattttga agatgacaat
    11401 atccgaacga tcaagcaagc ggtagccaag gtgatagata aaatgcagga gctatttgat
    11461 tcagaaaaaa aacaagtatt gattagccat ttttttgtaa tgggaagtgt aaaatctgat
    11521 tcggaaacga aaatcgaagt aggcgggttg gattcgatcc ctggtgaact acttacaccg
    11581 tttgattatg tagcactagg acatttgcat aatcaagctg ctttgagaca ggataatgct
    11641 cgctatagcg gttccccatt gaaattctct ttatctgaga tcaaccaaaa aaaaggcgta
    11701 tggatcgttg acctgacaac aggcctggag ttgaatttta gggagatcga acccctgcat
    11761 gatattgaag aaataacagc gagtttcaaa gaattgctgg atcctgcctt ttatcagtcc
    11821 gtcaatcggg agaattacat ctatatctat ctcactgata gaacgatcat cccgaatatg
    11881 atgaaccagc ttcggcaaat atatcctcgt attttgggga ttgaacgggc aaacggtcga
    11941 gcagaacaaa caaagcgcaa acgaaaagta ctagaaatga aagatcctca aaaattagca
    12001 gaagcatttt ttagtgaaat gacggaagag cagctaactg caaaccaaga aaaatggctc
    12061 ggagaaacgt tgcaagaaat catggaaatg gaatgatcaa gaatgaaacc gaaaaaatta
    12121 caacttaaaa attttggtcc ttttatcaat gagaccgtag atttttcacg tttgacagaa
    12181 gcaccgcttt ttttgatcag cgggaagaca ggagctggca aaacgacgat ttttgatggg
    12241 atcaccttcg cactttttgg tgaaacttcc ggaagacttc gctctggaaa agagatgcgt
    12301 tctttgtttg ctacaccaga cgaagaaaca agcgtgactt tttcttttga acatcagcag
    12361 atgagctatg aaatcgaacg aaaaccagaa caagttttag caaaacgaaa aggaaacgga
    12421 acaaaaaaac aggcagccaa agtaacgttg accatttttg atgggaaagg aaaagaaata
    12481 aaacaaatca caaaacgtac agaagcagat cagttgatca gagaactgat gaatctcgat
    12541 gcgaaacagt tttctcaaat cgttttacta ccccaaggag aattccgtaa ttttttggtt
    12601 tcatcaagca gtgataaaga gactgtttta cgtcatttat tcggtactca atttttccaa
    12661 cagttcaatg agcgattaaa agaaaaagca aaacaacagc aacaaaaatt ggatcattta
    12721 gagcaagagc tgcatcttct gcaaaaaagg tttgttttag tcaaagaaga ggaaccagca
    12781 gcagggtttg aagccgtttt agcccaatgg acaaaccatc aggcaatact cgaaaaacaa
    12841 atcacggaag aaaaagaagc tctaacggaa cttcaggaaa aacaaaagca actagaaaat
    12901 tcctactatc gttttgaaag cttgaaaaaa agttatgaag aaaaaacgat attaataaac
    12961 aagcaaaagg aattagctgc tcaaaaagac gaaatagagg aaaagaaacg atggattggg
    13021 tattatgaat ttacgcaacg attagtcgaa ccaatccagc gcctacaaga aataaaagtt
    13081 gaaaaaatag aactgtcaca aaaaagtagt gagcaaacaa agaaactagc gatgactacg
    13141 cagaattatg aagagtggca aaaaaatgaa gcaaaccgcg tacgccgaca aacaaaaatc
    13201 aaagaagctg ctcaaaaact gcaaaacaaa cagatgatgg tgccaattgt cgaatcctta
    13261 cgtagtaaaa aacaagacgc agaacaatta acaatttcat tggataaaga agaattgaat
    13321 gccgagaagc tgtatcaaaa tctcgctatc tatcaggaaa gaactcgaat tcttcaagaa
    13381 aatttactgg atcaggaaaa actgcaagaa gatcgtcttg cttttggaca gttaaaatat
    13441 ctagatgaaa agcagcaaga aatagaaaaa caaaaagcaa aagacactgg gtcgctcgca
    13501 cagataagag aagaactcaa aaaagcaact gaagaaataa caaaaaatag tcagctattg
    13561 aaagaacaga cagaacaagc agaacagctg aaaagccaat gggcaaagca gcaaattgct
    13621 cgtcttcagc tttttttgcg tccaggggag ccatgtcttg tttgtggatc aacggaacac
    13681 cccatacaac aattaaaaca cgaacagcca aataaggaag atatcttaaa agtagaacaa
    13741 caactggcag aagctgagtc tgctgtagaa agaactcagc aaatcattgc caaaagtgaa
    13801 gcacatgaaa ataatcttca acaacaatta tcagatttag aacaaaaaat catggcaacg
    13861 aatatacaat tggctgaaca agaagaacac ttgcaacagg ctttgaaaaa ggcatttcct
    13921 gagataacag aacaaaaaac aaaaacaact cttgtagtaa tcgaacaatc tttagataga
    13981 cgaaaaagca agatggccaa tgcaagaata gagctggaag aaataagaca acaagcagaa
    14041 aagctcgaac aacaattagc tgtctacaaa cagcagcaga cagaaaaaaa taatcagctc
    14101 cagcaactgc aaggagaaat caaaagttta gaacaacaat tgcaagatac ggatagtaac
    14161 tgtttagaag aggagatacg acgacttgaa aaacagttag aagaagactc gaatcaacta
    14221 gctgttgagc agaaagcagg agtgcaattg ttgcaagatc ttcatacgct tcggacacag
    14281 cttgatcttc aacatgaaca aatagaaaaa atagatgaaa aaatagaaaa ggaacaagaa
    14341 agaatagcta aaaagttaga aagccagtcg tatttttcaa caattgaaga aatctgtgca
    14401 tttgcacaac agcaagcaca atataaagaa aaccaacgta cagttacttc ttatcatgaa
    14461 gagcaattag tgacagctga ccggctgcgt caattagaaa atatccagct aactgagaca
    14521 ttcccagata cagaagagat cagccaacag ttagaagaaa taagacgtat gatcgaaata
    14581 aagcaagaaa agatatacgg tttacaagaa caaaaaatta gtaatcaaaa aatctacgat
    14641 gaatgtcttt cgatttatca cacaagccag acgaaattag atgagcttac gcaattacag
    14701 cagctttcgc aaacattcaa cggagaaaat cctaagaaga ctagtttgga acggtatgtg
    14761 cttcaggttt atcttcaaga agtgttacaa gtagcaaatg atcatctcca acgtttaacg
    14821 aaaaatcgtt atcaatttga gctagcggat accattggca gttatcgagg gaaaaccgga
    14881 ttagaaatca atatctatga tgatcaggca ggtatgtctc gaagtgcgca taccctgtct
    14941 ggcggtgaga gttttattgc agcactttct ttagcattag ctcttgccga tgtgatccag
    15001 acacaagctg gtggtgtagc aatcgatgca ttgtttattg atgaaggatt tgggtcatta
    15061 gatgaagaag ctttggaaat ggcaatggaa gctttagaaa cgatcgaaag tgaaggaaga
    15121 atgatcggca tcattagtca tgtaagagaa ttgaaagaac gagtattaca acaaataaga
    15181 attgatacag aaggtagcgg acaaagcaaa atacgctatc aacttggata aaaagggaga
    15241 ttggtaacta tgaaatggag tattccagaa aaagtcattg cacgtggacg tcaatacttg
    15301 caagaaggcc gagtgttatc ggttgtccct gaccaagaag aaaacgtgtg gcatgcagaa
    15361 gtcttgggca gtgaattgta tatggttgat ttggatggta cagcaaaaga gatagattac
    15421 tgccaatgtc cctattggga ggaacataaa tattgtaagc atacagttgc tgtggaactt
    15481 tatttacggt cgaaaaatct atctcgtatc atgaaaaaag accagccaat gctagaaccg
    15541 gtaaaagcaa caagtgaggg agaaatcatt gcaaaaggtt tttctcgtct aaaaacacaa
    15601 aacaatcctc aagcggtgca acctttgatt attgaatacc aagtagaaac gattgagact
    15661 aatcagtatc acccagagct ttctattttg gcactttact tgcgtatcgg ttcattggca
    15721 tcaccgaaaa aaacatatat cgtaaaaaac atctatgatt ttttacaagc ttacacagaa
    15781 aaagaaactt acactgttaa taaacagtac cagtttcgtc tagataaacg ggcgtttcaa
    15841 aaagaagatc aaaaaatact cacgcatctt gcaggaagtg ctcagacgca tcaattatta
    15901 ggtacgaacg gcttacaagt caaaggaaaa ctagataaaa gatatctgct tctcccagta
    15961 gaacaagccc gcttcctgtt agagcagatg aatcagacta cacgtttgca tctgcagata
    16021 aatgaacaaa acgatcaagg aatacaattt tccgatcaaa acaaaccttt atcattcaaa
    16081 gtggaaaaat ttggagaaga ttatttgctg caagcattga atgattttga ttttttcttt
    16141 atgcattatc agtggggagt tatccaaggg acattttatt ctctgacgaa atatcaacaa
    16201 acggtttatc aaacttggaa acaattgatt cgccgattgg agaaaccaga agtagtgttc
    16261 aagaaaaaag aattaccgtc tttgtttaaa gagattatcc ctctgctgtc agagatcggc
    16321 gacgtagcta ttgcccctgc agtagaagag gaagtagcag atatccccgt cgaatttatt
    16381 ttctttttcc gaaaagcaaa aggaaaaatc caagctcgag tcgatttcgt ctatggcgaa
    16441 gtcatctatt cgactgaccc gaagcatgag gtctcgacag aacaggcttt tcaggtgtta
    16501 cgtgacacag tagaagagaa acgagtactt gatctattca gaatgtatcg ctaccagaaa
    16561 aatgaaactg gttatgaacg tccattgcca agcggggaag aactatatgc ctttttccgc
    16621 acagaactat ctgtcttccg tcagtttggt gaagtcagac ttggaagaaa attgcgagag
    16681 ttgtatttag atgcgcataa acatcaaccg aaaatcgaag taaccgagac cggttcatgg
    16741 ctcgatattc gattcgatgt gacagggata gaggagcaag aaatcgatca tgtcctgcaa
    16801 agcttgctga gaaatgatgc tttttataca ttagaaaatg gtcaggttct ttcttttgat
    16861 tcagaagaat ttcaacaaac aagccaagtg ttgcaacaat tacgggaatc gatacgtaca
    16921 gaagaaggaa cgatccatgt acctaaaaat caaggattga tcattcaaaa tcaattggaa
    16981 aaaagcaatg ctacttttag tgaatcgttc caaacgatgg tacaagatct cattcatcca
    17041 gaaagatacc aggcacagct tcccaaagga ttgaatgcaa cgatgagaga ttatcaaaag
    17101 caaggttttc gatggttgaa gatgttaggt cattatcagt ttggcggtat tttagccgat
    17161 gaaatgggac ttgggaaaac actacagaca attgcctttt tactttcgga aaaagaagaa
    17221 cggaaaagct tctctgcttt gatcgtagcg cctgcgagct taatttacaa ctggcaggca
    17281 gaggtgagaa aattcgcccc ttctttaagt atccaagtaa tcaatgggaa taaaaaagaa
    17341 cgtgaagaat tactggctaa agacacagat atccgagtga cttcttatgc cagcttaaga
    17401 caggatctag ctaattatca gtcgcaaaaa attgattatt tgattcttga tgaggcacaa
    17461 atggtcaaaa atagcagtac gaaaactgca caagcattga gagaattagc tgtacctcag
    17521 cggtttgcac tgagtgggac accaatcgag aataatttag aagaattgtg gtcattgttt
    17581 gcaacgatta tgccaggatt tttcccgact aaaacaaaat tcagggaact tgccgcagaa
    17641 gaaattgcgc aaatgattcg accgtttatt ttacggcgag ataaacaaac tgtgttgaaa
    17701 gacttgccgg aaaaaacaga gatgaatcta tattctgcac ttacagaaga acaaaaaact
    17761 gtttatttag cttacttgcg tcaaatgcgt gaagaaatca catcaatgga ttctgaagca
    17821 ttcaagaaaa accggatcgg gatacttgct ggtctgacaa gactgcgaca aatttgctgc
    17881 gatcctcgtc tgtttattga agattaccaa ggcggttccg gcaagctcga acaagtaaaa
    17941 gatctactgg tagctgccaa agagaacaaa cgaagagttt tgctgttttc tcaatttaca
    18001 gggatgctga ctatcttgca agaagagtta gccgagctag ggatctcgac attttattta
    18061 agaggcagta cgaaaccaca agatcggtta agtatggtag acgcatttaa tgctggcgaa
    18121 aaagatgttt ttctgatttc cttaaaggct gggggaactg gattgaatct cacgggagca
    18181 gatacggtca tcttgtatga cctttggtgg aatcctgcca ttgaagaaca agctgctgga
    18241 cgtgcacatc ggatcggaca aaaaaatgtg gtagaagtgt ggcgtatgat ctctgaagga
    18301 acgattgagg aacgcatgga ttcactccag caagaaaaac gcgaactgtt ccaaaaagtg
    18361 atccaaggta atgaagaaca gttgacgaaa ctgactgaag aagacatccg catgatttta
    18421 agtgtaggag aaatcgagga gtaagagact cgaaaaagcg ttcagtaatg ggtaagaaag
    18481 cctttctaaa gaagcgattg tttatttgat ttaaaaaata gcaaggaggc tgggattcca
    18541 ttttatccca gcttccttgc tatcattttt ttatttctat ttttaaaggt tatacaaaga
    18601 ttgtcttcat tgtctctaac agctgatcaa aagaagaaat ctcgatatcg caaattggag
    18661 tctgttcaat tttgcgttct cgatggttga accaaagcgc ttgccagtta gcactttttg
    18721 cacctagaac atcattgtcg taattatcgc caacgtataa ggtctcttct ggaagcatat
    18781 gaaattcttt ctcagctagc tgaaaaatct ctttttctgg tttttggaaa cctgttgctt
    18841 gagaaatgag catattgcgg gaaggaatcc aattgttcaa ttgaagttgc ttcagttttt
    18901 ttgtctgatg gtcagtcggc ccatttgtga taattcctaa tgggacattc ttttccttca
    18961 agaaatcgag ggtttttttg acctcctcat gcaagcagat attatcaagt tcttcctcgt
    19021 aaattttttg aaataagagg ccatcttctt ccgtaatatg agggtagtct aaatctttta
    19081 gtgattggct gatccgatgt gctcgcatat attctagtgt ccaatctcct gccattactt
    19141 ttgggaagtt ttcatcacta tgatggcgga accggatata tagtggatgc atatctgcat
    19201 ctgaaacaag cggaacgact cttttgaccg cgtttcgaaa tggctgctgc tgatcataca
    19261 tcgtatcatc aacatcaaac acaactgatt taatcaaaat tttacacgcc tttcggtttt
    19321 cattgatttt ttcacaaatc cttgactatt gtaacagaaa aaattgtcta aaaaagctga
    19381 taaataaagg tttttcttac tatatttcta tttccttgac agaataaggt ttttatattt
    19441 cagttttttt cactaaaaat cgtgaaacac gtcaataagt ttgatgaaca aattaatcat
    19501 tatggtagaa gtcgattgat ttattttaat gcaatctctg tggaattttc gaaagattta
    19561 tttttttaat acagtctcaa tataactatt caaacataaa ataacgttat ttgatttatt
    19621 tgtttatagt aaattttatt tagtaatcat tgctttaaaa tactacgctt atataacaga
    19681 gaaaataagc gttataataa agaacagtat tatttaaaat gacagaaaac attactatta
    19741 aagaaataaa aaacata
//
LOCUS       DAYYHX010000038        19441 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000038 DAYYHX010000000
VERSION     DAYYHX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.038"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..3644
                     /locus_tag="ACHA6Y_000976"
     CDS             117..3644
                     /locus_tag="ACHA6Y_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8987089.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3641..7363
                     /gene="addA"
                     /locus_tag="ACHA6Y_000977"
     CDS             3641..7363
                     /gene="addA"
                     /locus_tag="ACHA6Y_000977"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM8987090.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7432..7773
                     /locus_tag="ACHA6Y_000978"
     CDS             7432..7773
                     /locus_tag="ACHA6Y_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8987091.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8082..9125
                     /gene="pheS"
                     /locus_tag="ACHA6Y_000979"
     CDS             8082..9125
                     /gene="pheS"
                     /locus_tag="ACHA6Y_000979"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM8987092.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9130..11550
                     /gene="pheT"
                     /locus_tag="ACHA6Y_000980"
     CDS             9130..11550
                     /gene="pheT"
                     /locus_tag="ACHA6Y_000980"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM8987093.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11720..12730)
                     /gene="trpS"
                     /locus_tag="ACHA6Y_000981"
     CDS             complement(11720..12730)
                     /gene="trpS"
                     /locus_tag="ACHA6Y_000981"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM8987094.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12984..13721
                     /locus_tag="ACHA6Y_000982"
     CDS             12984..13721
                     /locus_tag="ACHA6Y_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8987095.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13735..14550
                     /locus_tag="ACHA6Y_000983"
     CDS             13735..14550
                     /locus_tag="ACHA6Y_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8987096.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14563..15210
                     /locus_tag="ACHA6Y_000984"
     CDS             14563..15210
                     /locus_tag="ACHA6Y_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8987097.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15226..15882
                     /locus_tag="ACHA6Y_000985"
     CDS             15226..15882
                     /locus_tag="ACHA6Y_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8987098.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16044..16865
                     /gene="racE"
                     /locus_tag="ACHA6Y_000986"
     CDS             16044..16865
                     /gene="racE"
                     /locus_tag="ACHA6Y_000986"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM8987099.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16868..18223
                     /gene="rph"
                     /locus_tag="ACHA6Y_000987"
     CDS             16868..18223
                     /gene="rph"
                     /locus_tag="ACHA6Y_000987"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM8987100.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18224..18742
                     /locus_tag="ACHA6Y_000988"
     CDS             18224..18742
                     /locus_tag="ACHA6Y_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8987101.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18849..19349
                     /gene="cbpB"
                     /locus_tag="ACHA6Y_000989"
     CDS             18849..19349
                     /gene="cbpB"
                     /locus_tag="ACHA6Y_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM8987102.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6538 a         3339 c         4176 g         5388 t
ORIGIN      
        1 aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag aacaaatgtt
       61 cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg aaaagcatgt
      121 ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg atcgacgaag
      181 catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt cccaactaca
      241 gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc ggaaaaaaaa
      301 ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg tatttgctgc
      361 aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca atgatcttgc
      421 gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa atcaataaaa
      481 gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga aatattcaac
      541 cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag caacttaaaa
      601 tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa cgtgcactaa
      661 caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat ttcaacaacg
      721 tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa ttactgcagg
      781 caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct tatacagcgg
      841 atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc cgtctttttc
      901 aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca gagcagactc
      961 gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc caaaagaaag
     1021 cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct gaaaatccaa
     1081 ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa gaaaactacc
     1141 gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat atccttcggc
     1201 cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg atggaaaatc
     1261 atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct tatcggctaa
     1321 gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca gaaaccgaac
     1381 ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc actgaaaatg
     1441 tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac tggcagtttg
     1501 tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa gaacagtcaa
     1561 atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa ataaagaaaa
     1621 gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa agtggcgtac
     1681 agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta gaagcggcaa
     1741 gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat gtagtaattt
     1801 atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt ggtctagaaa
     1861 atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat cgtttggaat
     1921 tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat caagtgtttc
     1981 cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta ttaaatcagg
     2041 ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct tttgagcctt
     2101 tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt tatgcacaaa
     2161 gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc acagaatatt
     2221 tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct aattgttatg
     2281 tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta gctttagatg
     2341 aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg aattcgtcat
     2401 ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt cctgtttcga
     2461 tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca atttcacgta
     2521 tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg aaattgaaag
     2581 aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat gaatcattgg
     2641 atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg tccgatgcgc
     2701 agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt cggtttgaga
     2761 tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag acgattcaaa
     2821 aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct cttcagacag
     2881 agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa ttaccgttga
     2941 gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta gcttctgaac
     3001 aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca ttcgatgtca
     3061 cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta gcattgatgg
     3121 atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat tatttacatg
     3181 ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg aaaaaattta
     3241 gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat cattcactgg
     3301 aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat gaataccaaa
     3361 aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta ttcatggcgc
     3421 ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa atcaaactaa
     3481 atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc agaagcatct
     3541 gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa atagataagg
     3601 aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa ttcctttaaa
     3661 accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc aaggagataa
     3721 tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc gaagagtgat
     3781 tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat ttactgaagc
     3841 tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg tcaatagcga
     3901 aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc ccacagcgaa
     3961 tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt acttaattga
     4021 tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga tgaaggagga
     4081 tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat ttttccaatt
     4141 aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg tgttttctct
     4201 ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt taagcgataa
     4261 ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa tccgtccttt
     4321 agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc agttagcaca
     4381 aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc agatcaagaa
     4441 tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt tggaacaact
     4501 aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt ccaatgaagt
     4561 caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa gttattttcc
     4621 tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag tcgaagaaat
     4681 gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg aaaaaggtgt
     4741 gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa aaacgaaaga
     4801 tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag aagtaatggt
     4861 agatgaatat caagacgtga accaattaca agaagccatt ttatactggc taagagaacc
     4921 cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca tctattcttt
     4981 ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa agaaagaagg
     5041 cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc tttcttttac
     5101 gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt atgacgaagc
     5161 agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg agccggaaat
     5221 catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg atgatatact
     5281 ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc aattaattga
     5341 ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag agtacaaaga
     5401 cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga ttttcaaaac
     5461 attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga cagaaattcg
     5521 cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc cgcttgcggc
     5581 tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca ttcgattagc
     5641 taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa acgaagatga
     5701 attagcagct aagctggaac attttggcaa gcaactagag cattggcgag aacttgctcg
     5761 ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat atctagagta
     5821 tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat tggttgatcg
     5881 tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc gtttcattga
     5941 aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag aagataatgc
     6001 tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg tatttttgtt
     6061 agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt ttgaagaaaa
     6121 acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg atacgttacc
     6181 gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa tgcgtaaatt
     6241 gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct ataaaaataa
     6301 agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag tttttgatcc
     6361 tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg gattgatccg
     6421 tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc ttcaacattc
     6481 aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac aaagacagtt
     6541 gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg aaactttatt
     6601 agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt cttcacaaac
     6661 cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag atgatacaca
     6721 agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc aatttcgtta
     6781 tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag tgtcagcagc
     6841 tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga tgccaactac
     6901 agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag acgaaaaagt
     6961 cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac ttggtcaaca
     7021 gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc tgcttcctgc
     7081 tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc acggaatcgt
     7141 cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa cagattttat
     7201 tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc aaggacagct
     7261 aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa atgttttcct
     7321 aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat tgagcaaact
     7381 tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat gatggagcta
     7441 acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat tttaggaaaa
     7501 aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg ctttgttgag
     7561 cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt gaaggaatta
     7621 gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg ttcagaatat
     7681 ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca agtttggagc
     7741 gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct tgtaaactat
     7801 agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta aaccagttca
     7861 gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt tcactttccg
     7921 agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt gcggtgtaag
     7981 tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca ggaatgaggt
     8041 ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta caagcacaat
     8101 tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat ttaaatgaat
     8161 taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa gtattacgtg
     8221 ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca aatgagatcc
     8281 gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa gaagcgttaa
     8341 atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa gtaaaacatg
     8401 gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt gtgggtatgg
     8461 gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc gaacggatga
     8521 atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt tcagacgaga
     8581 tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa catgatttct
     8641 ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat acagatgatg
     8701 cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa aacatcacaa
     8761 tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg gaagatcgtg
     8821 aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa gtagatgtca
     8881 gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga tggatcgaaa
     8941 ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc gatccagaag
     9001 aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg cggtatggag
     9061 tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag ttcaaggggg
     9121 agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt atcgaatgta
     9181 acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga gggagtaacg
     9241 gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg cgtaccacat
     9301 ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga actgtctcaa
     9361 atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc acttcctaac
     9421 tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca agtatcaaat
     9481 ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc gaaagaatac
     9541 gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt ttttccttat
     9601 ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc ggatgcattg
     9661 tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc tacattcgat
     9721 gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt ggaagttgaa
     9781 gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag aatccagcca
     9841 agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat caacaatgta
     9901 gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc atttgactac
     9961 gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga aacattagta
    10021 acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac taacggcaaa
    10081 gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac gaatgaaaca
    10141 acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg tacagcaaaa
    10201 caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa ccatgcgact
    10261 gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg tggggaagtt
    10321 ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt gaagatttca
    10381 cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt aaatgagatt
    10441 tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt agtagtacca
    10501 ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc tcgtatttat
    10561 ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg aagcttgtca
    10621 aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg attgagcgaa
    10681 gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat aaatgaaagt
    10741 aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg aatgaatttg
    10801 atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac agagattgct
    10861 ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca tctgccgcaa
    10921 gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga ttggcagacg
    10981 aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt atttgaacaa
    11041 ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga aatgcatcct
    11101 ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg tcaagtgcat
    11161 ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt tgatttatct
    11221 gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa attcccagca
    11281 gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga attaacagaa
    11341 gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga tgtctatcaa
    11401 ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt cgtcaaccca
    11461 gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga aaaagcgttg
    11521 gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta acgatcaagt
    11581 agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca catctctttt
    11641 tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct ttacagcctg
    11701 cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat tttacttcag
    11761 aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag atttccataa
    11821 cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca aattcagctt
    11881 ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg cggcggtaat
    11941 gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag taggtgaaga
    12001 ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga tcagtataca
    12061 tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata ccgtttccta
    12121 acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt ccttttggtg
    12181 ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag ctttgtacga
    12241 tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca agattcgctt
    12301 tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct gtaggcacgc
    12361 tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc cgaacgcgtc
    12421 ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga atttgtgatt
    12481 ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct aaagcaactt
    12541 gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt gcttgcatgt
    12601 cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact cgggttttta
    12661 gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc gttaaaataa
    12721 tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa tatcttaccg
    12781 tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg ataaaataac
    12841 taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag aaactttaca
    12901 aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga aatcttacta
    12961 aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg gataaatatt
    13021 atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga gaagttgtcg
    13081 ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc aatggattag
    13141 agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat aaaaagacaa
    13201 aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat ttgtacccaa
    13261 ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag caagaccagg
    13321 caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta gataagaaag
    13381 attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc gccagaggtt
    13441 tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg gatccggaaa
    13501 tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg tcaatgattg
    13561 tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc ttcatggctg
    13621 atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat ccaaatgaag
    13681 tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa aaagatgcgc
    13741 aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc actagccgga
    13801 tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc taccaatgaa
    13861 atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat tgagagcaga
    13921 actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat ccttggtgac
    13981 aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt attgaaaaac
    14041 ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa aaaacaggtt
    14101 gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa aggaagccaa
    14161 atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa aggttctaca
    14221 tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt agaaaactat
    14281 gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac ggataatgcg
    14341 attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg aacgtttact
    14401 aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa agctgttaac
    14461 caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca aaaatggttt
    14521 ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga attgatacaa
    14581 acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag tgtcttagca
    14641 cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc aaagaataaa
    14701 gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc attattaatc
    14761 atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga tggattccaa
    14821 gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac agtacgttca
    14881 gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg ttttacctat
    14941 tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat cccgccatta
    15001 ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt agctggttta
    15061 gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt cgatacgtat
    15121 atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt aatggtttat
    15181 cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga ttttagcggc
    15241 gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac tgttgaagtt
    15301 tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt gattcgtttt
    15361 gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat acggaatctt
    15421 ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg cattcagatg
    15481 aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat gttgtcggaa
    15541 attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg attatcaacg
    15601 ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt taaatcaatg
    15661 atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt agcagtaatc
    15721 atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa caccaattat
    15781 gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta cgcgctttct
    15841 ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat tcttttagcg
    15901 gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa aagagatttc
    15961 gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca caatagggag
    16021 gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct atcggattta
    16081 ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa ttaccgaatg
    16141 aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga cccgcggaac
    16201 aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga atcaagatgc
    16261 tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa gctgctcttt
    16321 ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa aaaacacaaa
    16381 ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct tatgaaaaag
    16441 cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc aaatttgttt
    16501 cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg gcagaaacat
    16561 tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc cattatccat
    16621 tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc gattctggag
    16681 cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc aattcaccgc
    16741 aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt ttcgaggaaa
    16801 tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa ttgggaggaa
    16861 aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg agcttcaaac
    16921 aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata caacagttat
    16981 ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag gaacaggatg
    17041 ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc gtagagaaag
    17101 cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg gtcgttcctt
    17161 gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag actgcgatgt
    17221 gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg tggcattgaa
    17281 attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa taaaagaaca
    17341 tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag atttggatta
    17401 ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat ctggaaaatt
    17461 cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt tgaatgaaat
    17521 gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac atgctttgct
    17581 aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca tcattgcgac
    17641 aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg gatatcatgt
    17701 aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca gcacatttga
    17761 agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc cagtcttagc
    17821 agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct attctgcacg
    17881 ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat atgaattgac
    17941 agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat tcgcggcacc
    18001 caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc gtataccatc
    18061 tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga aacaaacagc
    18121 agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag caatgaaaaa
    18181 attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt atttagtagt
    18241 aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt atcgttctga
    18301 agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc cattatggga
    18361 tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac agaaaaaagt
    18421 gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga atgtccgatt
    18481 tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg tgttttttgg
    18541 tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga atcctggtag
    18601 catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg aaagtttaga
    18661 tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag agttggcctt
    18721 ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa taagctttat
    18781 tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat agaaatggag
    18841 gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag gaaacgtttt
    18901 tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc catggtttac
    18961 ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat gatcactttg
    19021 taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa acaatcagca
    19081 tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt ccgacaattg
    19141 atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct tttttaccgg
    19201 ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg cttaaagccg
    19261 taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa atcgaagaat
    19321 taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata atcaaaaaat
    19381 aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct gggacaaaag
    19441 c
//
LOCUS       DAYYHX010000039        18924 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000039 DAYYHX010000000
VERSION     DAYYHX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.039"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..744)
                     /locus_tag="ACHA6Y_000990"
     CDS             complement(34..744)
                     /locus_tag="ACHA6Y_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM8987103.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(762..1259)
                     /locus_tag="ACHA6Y_000991"
     CDS             complement(762..1259)
                     /locus_tag="ACHA6Y_000991"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8987104.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(1296..1748)
                     /locus_tag="ACHA6Y_000992"
     CDS             complement(1296..1748)
                     /locus_tag="ACHA6Y_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8987105.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(1883..2488)
                     /locus_tag="ACHA6Y_000993"
     CDS             complement(1883..2488)
                     /locus_tag="ACHA6Y_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8987106.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(2938..3558)
                     /locus_tag="ACHA6Y_000994"
     CDS             complement(2938..3558)
                     /locus_tag="ACHA6Y_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8987107.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(3560..4429)
                     /locus_tag="ACHA6Y_000995"
     CDS             complement(3560..4429)
                     /locus_tag="ACHA6Y_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8987108.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(4452..4859)
                     /locus_tag="ACHA6Y_000996"
     CDS             complement(4452..4859)
                     /locus_tag="ACHA6Y_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8987109.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(4878..6167)
                     /locus_tag="ACHA6Y_000997"
     CDS             complement(4878..6167)
                     /locus_tag="ACHA6Y_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8987110.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(6194..6496)
                     /locus_tag="ACHA6Y_000998"
     CDS             complement(6194..6496)
                     /locus_tag="ACHA6Y_000998"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8987111.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(6521..8614)
                     /locus_tag="ACHA6Y_000999"
     CDS             complement(6521..8614)
                     /locus_tag="ACHA6Y_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8987112.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(9103..9867)
                     /locus_tag="ACHA6Y_001000"
     CDS             complement(9103..9867)
                     /locus_tag="ACHA6Y_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM8987113.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(9978..10847)
                     /gene="mdcB"
                     /locus_tag="ACHA6Y_001001"
     CDS             complement(9978..10847)
                     /gene="mdcB"
                     /locus_tag="ACHA6Y_001001"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM8987114.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(10872..11948)
                     /locus_tag="ACHA6Y_001002"
     CDS             complement(10872..11948)
                     /locus_tag="ACHA6Y_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8987115.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(11972..12565)
                     /locus_tag="ACHA6Y_001003"
     CDS             complement(11972..12565)
                     /locus_tag="ACHA6Y_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM8987116.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(12569..14245)
                     /gene="mdcD"
                     /locus_tag="ACHA6Y_001004"
     CDS             complement(12569..14245)
                     /gene="mdcD"
                     /locus_tag="ACHA6Y_001004"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM8987117.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(14235..14534)
                     /locus_tag="ACHA6Y_001005"
     CDS             complement(14235..14534)
                     /locus_tag="ACHA6Y_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM8987118.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(14636..16282)
                     /gene="mdcA"
                     /locus_tag="ACHA6Y_001006"
     CDS             complement(14636..16282)
                     /gene="mdcA"
                     /locus_tag="ACHA6Y_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFM8987119.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(16289..17200)
                     /locus_tag="ACHA6Y_001007"
     CDS             complement(16289..17200)
                     /locus_tag="ACHA6Y_001007"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8987120.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            17320..18177
                     /locus_tag="ACHA6Y_001008"
     CDS             17320..18177
                     /locus_tag="ACHA6Y_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8987121.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(18364..18726)
                     /locus_tag="ACHA6Y_001009"
     CDS             complement(18364..18726)
                     /locus_tag="ACHA6Y_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM8987122.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         5257 a         4025 c         3279 g         6363 t
ORIGIN      
        1 gcaatgaatg ccgctcttct tatttcgatt cttttataat tctgcttctg attcatcttt
       61 ggttagtgtg tgtctaacac tagttacgcc aaatgtttga tcgatttttt ccttcaacgc
      121 ttcgattgcc tgtgcgccac gtgccgcttg ttcagcattg atggattcaa tgaccagcac
      181 cgctgcttgt gtttcttccg tcagcatcgt tctttgggct tcacgccagt tgagacagcg
      241 acagatcgct ccttgttcat catagtagat gatttcttcc ggaagtgccg gagcatcgga
      301 atctgcacct aaaggcagga atgattcgcc accttgggct tttcctaaat gcagatttcc
      361 tgcgatcttt tcgatatctt ctccgccgca aggtacgcca aaagacaagg aaatactatt
      421 atagatatct acaagcggat tgattgggaa aaattcccgt tcttgatttg cacgtttcag
      481 taaagcttca atagaagaac gtgcaccttt tttagtcttg aatgtggtaa atgcctgacg
      541 ccactgagca atgacttcgt tttggctgaa tgtttcttct gtaaggtaat ttcttgcttc
      601 ttttgctgcc tcattcaata attgatgata cgtttcttca ctttcttttg ttacgtggtt
      661 atcgatacca tttacggata agatttcgat ggttgcttca ggaaatactt cccagaaatt
      721 ggtatccact attacttttt tcatctgttc acacctcttg attagaataa ttgcttgatg
      781 aatagatagt ctgtttgggg atcatctccc atataaaaaa catgttcgct gtatttgata
      841 aatccttttt tacgataaaa agcgagcgca acatcgttgt gttcccaaac accgagccaa
      901 atggctgttt tttgcttttc ttttgctttt tcaatagctg tatctagtaa aaatgtaccg
      961 ataccttgat gtttaaattt ttttcgtaca tagattcgct cgatttctaa aaaatgatca
     1021 gccatttcct cagattgact cgttcctgta tttagtttca ggtaacccgc aggctcttga
     1081 tcgatactca ctaggaaaaa atcggtttct ttatctttta gctcttctgt aagtttttct
     1141 agtgcataag cttgtgtcaa gtaatcagct aaatgctccg gtgaattatc ttttccaaat
     1201 gtatctgtaa atgtttcgat actaatttgt tgtagtaagt ctacatctga aggcgtcatt
     1261 tttcgaattt cgtatttatt cacggcttct tcctttcaat agatcctttt attgcctttt
     1321 ttgacaaaat tccaatcttc gctaacattc ttgcatactt tatttaaaag atattccaat
     1381 tgttttgctt cttcgtcaga aagaccattt aatgcgactg cattggagta gttgttttct
     1441 cgaatgatga aaggatagat ttctgctcct tttttagtcg gatagagttt cttgattttt
     1501 ttattttctt tatcttccaa acgttcgatc atcccattgg attctaattt cttgattgct
     1561 cgggcagtcg ttgtacgatc tactttgatc atttctgcta gtttttcttg gatgattcca
     1621 ggattttcgc aaatacgtac taaataaaga tactgtcctc ttgttaaatc gacttctttg
     1681 aattcgatat tgctaataga atctagcgct cgggcaatca ttccgatgtc tcgaaggatt
     1741 tcagccattt catcccacct ttattttgtt tgtaagacta gttcacctct ttttgttgca
     1801 aatgcaacta ttttcaataa aaaaatcaag ttctttttta ataactagta aagtatattc
     1861 ttttcttctt ttacgaaaaa gctcaatcac aatagatctg gtttttgttt tctaaaataa
     1921 gtaactgttt cttcgttgtt cgcttttctg atagctttgg caactcttct atactaaaaa
     1981 atcccatgtt ggaagtttca ttattctcaa taaacttggc ctcttcaccg tggatcgcac
     2041 aagcaaaaac aagtttataa tactgaaaaa gctgtggaat atctttttgt ttattcgtat
     2101 caaaaactgc tcgcaactga gtagtttcta ctgttaatcc tgtttcttca tagacttctt
     2161 tacgcacatt ttcttcagga gacaatccta tttcagcaaa tccaccagga agtgaccatt
     2221 ctttcgtccg tttatcttcg attagtagga ccttgccatc ttttttgata taggcacgaa
     2281 catctacttt tggtgttgga tgtccttctc caatcgttgt gagcgctttt atttcttcga
     2341 aagtttcttt tcctatttca ctgatcagtt ccaatgaaat cgtccggatc tcctcaaaac
     2401 gctctttatc aaatacatct tttccataat atagaccagc ttgagaaagt gcgagtagtc
     2461 ttttgtattg agccaacttc ttgttcattc tcttcccttc ttttttagca aatgtatgac
     2521 tatgtaatca agtatagaag actggtttaa ttacggcaaa taaatataat gaaatctgca
     2581 ataaataaaa gaatgttaaa aaactcgaga acaaaagaca ttcgacttct ctggggctgt
     2641 gaataaatgg cgttcaaaag cttgcatctg cggtaataag ccccaccaaa cgaaaaatat
     2701 gacgagctat tcttgtctgg ctgttcttat taattgaggc aggacagctt tttcacaacc
     2761 tccccactaa acggtgttct cctaccaact cctcggtcac aagtcacaat attcatcaaa
     2821 catgagaagc tgtttttttg aaattgctcc ttgtgactcg gagctgaacg acaggctcac
     2881 gacctctcgg taaacagcgt tcaaaagcca atttttcgat attaagccat ttctttgtta
     2941 cttattgtca ttgatttctt tttctaattt atccaatagt ggagtgtaat ccacttgagt
     3001 atagtctgtt acatcaaatt catatagtgt tcctaactgg aatttgtcat attttcggtc
     3061 ggcgatgatc tttttgaatg cttctttcgt aatatgattg gtccctttcg ttcgatcttc
     3121 caatacgtca ccatagtgat aatgatccat gatatagctc aaatgacgat ctgattcacg
     3181 atcacgtttg taccgacgtt gccataacac ttcaaagtct gctgttaggc gaatcgtgat
     3241 cacctgatat ccgtatctat ctgctaattc ttggagtcgt gctttttgct tatcactaaa
     3301 aggatactcg gacacaatca ctttctttcc tgcttccata tacaaatcaa gtgctttgta
     3361 atagaaagga taaactttcg tcctttcttg ttccgcccgt tcttccaaat tgttaaaccc
     3421 gatggattct gcatagtatt ccttgatttc atcgagcgtg atggcataca tatctggaaa
     3481 tcgttcccgt agtttattca taagatatgt ttttcctgta ccaggactac cagccagaag
     3541 aatgaaatac tttttcatct catttatttc ctaagttaat tggactttcc aagttcataa
     3601 acagtccaat cttatgcttg acaatttctt ttactgcttc atttgctgga atcacattat
     3661 ggcgaagaga cttgttgatt tctgtttcac taaaggtttc tttagctact ttcgtccagt
     3721 tgactaaaag ctccgttcca acattgaatt tgcggatccc gttattgatc aattcaggat
     3781 aatcttcttc tttcacacct gttcctccat ggataactag ggggacatca actactgcat
     3841 cgatttcttt cagcaaggga atgtttactt ctgtttttga tttgaattgt ccatgattcg
     3901 taccgatagc aatagctaat gcatccacgc ctgtagcttc tacaaatttc actgcgtctt
     3961 ccggtcttgt atataccttg tcattttcag cgacatggat tccttcttct gtaccgccga
     4021 ttgttccaag ttctccttct acggaaacac cgtgcgcatg ggcatattct actactgctt
     4081 ttgtcttcag gatattttct ttgaaaggca gatgggaccc atcatacatg acagaagtat
     4141 atccgctgtc gattgcttct ttgatatcat caaagtctcg ggcgtggtct aagtgcaaga
     4201 ctgcatcaac catttcttct tctgcaatag ctgtcattac attgactaac acatgcatgc
     4261 cgatatattt agcggtatcg acacttgttt gaataatgat cggcgaaccc atttcttttg
     4321 ctgcgcctag catagcagga agcatttcta ggttatgcgt attaaaagct ccaactgtaa
     4381 aattcaactc ttctgctgtt tttgttactt ctttcaatgt tgtatacatc ttttattgcc
     4441 accttatctt tttatttgtt tcctttgatc gttacttgtt ttgcaatttg tcgcatctca
     4501 tccattttgc ccatgtagta atcgatccct tgatcatcac cagcacgagc agcatctgca
     4561 cgcttctggt tatagaggct catcagtttt ttatacccat ctccgatcgt ctgtttattt
     4621 tccaaactct tttctaaata ttggatggct tgatcgttca catacaattc agaaaattta
     4681 agtccaactt gttcataaag tttcgctaat ttatgcttat cttgggtagt ggaaatctct
     4741 tgtttcaatt gtgtaatctc tgattgaatc ttttcggtcg cttctggcga tagggctttt
     4801 tcttcttttg ccagaacttt tttcggttct ttttcttttt tcttaaaaaa tccaaacatc
     4861 attttcccta ctttcaatta aaatgctttc aaaactgggc ttaagatcca ggcatataag
     4921 agtgctgcgg ctactgtatc tacatcggtt gctgtagcat tgttgaatcc catcgaatta
     4981 aagatcgtta ctagcaaagc gggcaataat gtaataaaga aaccatgagc aataccgcca
     5041 atgatcgctc ctcggcgtcc gccaactgcg ttgccaaata tccctgctgt ccctccggca
     5101 aagaaattgg ttagcatacc aggtaaaatc atcgctaagc cgaacatggg caaagtaaac
     5161 atggcaataa tcgtaccaat cgttgtcgta atgaaaccta aaatgacagc atttgggcta
     5221 taaggaaaga aaactggaca atctaaggca gggatcgcat caggaactaa tttcatagct
     5281 atccctcgaa atgccggcac gatttcacct aacagtagac gtacaccagc tagcaataca
     5341 tacacaccca ctacgaattg gatggcttgt aaaaaggcaa acatgatata gttttgatct
     5401 cctgataatt cagaagcgaa tggttcgcct gcaaataaaa cagtgatgat atatagcggg
     5461 accatcacga ccatgacaga caaataagta tcttgcagaa attcgaaatg tgtcggcaac
     5521 ttgatatctt cgatcgactt tttattttcg cctttctcac caaaaagctt agcaacaccg
     5581 gcttcaaata agtacccgat cgtacaaaaa tgacctaaag caatatcatt tgatccagtg
     5641 attttacgaa tgatcggttg cgctactgcc ggcatagcaa tggcgaaaca agctccgata
     5701 aaaccaccga ccaagatcaa tacgatccct cgcagaccgg caaaatagcc aaagactgtc
     5761 gtcatcgttg ccatccataa aattgcttga ccagttaaaa aaatgtactt ccattttgtg
     5821 aaacgagcca aaataatatt gaatacaaaa atggctaaga acgtcagtgc aatatctcta
     5881 cctaaaccca aatcattcat tgcttgacca ttgatagatt caatagaagg gatgatccct
     5941 tgcatatgaa atccttcagt aaagattttc ccgaaataag ttaaacttcc aacgatgatg
     6001 ctcgaaccgg cactcaacac ttgaaatcct aggagtgtct ttaacgtgcc agagatgact
     6061 tgtcctgctg atttcttctg cagtagtaga cccaacatag caatcaatgc aatggtgatc
     6121 gatgcctgag tcaaaatatt ttcgatgata aaattgataa cgcccatgtt tttaccctct
     6181 tttctttttg tatttagata actcccttat cttgcagaac tggggtgatt ttttcttcaa
     6241 tttcattttt tgaaacaata tttttcaaat agatcatcgc cgttttagga tcaactgcga
     6301 acttttcgaa ctgcgaacga aaattttctg ctgtgatgat cacatctggt ttcatggaaa
     6361 ctgcggaaga aatatcacaa tggtccagtt tggcttctac tcctaatttg ttcaaaacat
     6421 cctcagcaga catctgtgct gcaaaactac ttcctaaacc tgcaccgcat acgaataaaa
     6481 tgttcaattt gttcatcttt gttctccccc gtatcattta ttatttctca actactgaaa
     6541 aaagcagttg tttgaaatct tcgatatttg tgcattcaat caatcgatcc aacttctctt
     6601 cgttgttgat caattcaatt agttctttca tgatgttcag atgcgagtag gagtctactg
     6661 ctgccaaaca aaaaataatc tttactggat ccatttcagg attaccgaaa tcgattggtt
     6721 gctcaatcgt agctacgctg actcctaatt tattgacccc atcttctggt cgggcatggg
     6781 ctagtgccat gtgtttgcca atcacgatat acggtccgta atcattcact gcacgaatca
     6841 tcgcttcgac atagcgctct tcgacaatgt tttctttaac caatggtcta gcagctagtt
     6901 caatcgattc ctcccaagta gtggcttttt ctttgatcat gatattgctg tccgtcaaaa
     6961 tatcttttaa cataggctgg atctcccttt tgttgatttc taaattgttt gtttcgaata
     7021 atttttctaa cttgccatag atgattcgat ctactcggcc accactttct ttcatgacat
     7081 ctattatttg agtgaataac tccgtcgatt catgactgtt aggctctaac cgctgtagtt
     7141 cttcatttct tttcaaaaag tcttctataa ccaagatatt cgtctcagtt aaaatcggat
     7201 cgacaactaa aagcggctta tttcgataat tcaattgaaa ggtgctgaaa accagattga
     7261 catccaattt atctactaat tcgatttctt ttgaacttaa aactgcagta atctcgatct
     7321 gaggaaactt ttctttcaga ttctctgcta acagatttcc agtagccatc ccatggttac
     7381 agacaacgac tgatttataa acataattca agtctcgctt gattacactg gcgatcgtcg
     7441 aaaagtggat gacaagaaaa gctatctcgt cctccagtat cttgccacca attacttctt
     7501 cgatcgtagg aacaaaatga gtgactgcgc ggtagattgg gaaataattt gttcggatgt
     7561 tttctttcaa tggattcatg acttggatat gattcttgat tcttacaatc agtgcagcca
     7621 tgtgcttata gagattttcg aataaagctt cttcctttag atggaaaggt acgtgtgtct
     7681 cgttctctac aaattggatc aattggatcg ttagtaattg agcttgtacc cactcgattg
     7741 aattggagat atctctagca ttgaacgtct caatagtgaa acgaagataa tttctttcta
     7801 cttgcgggac attgtccaat tgaaattcct cgatcatgtg gtcaataaat gcagaaaagc
     7861 tactgtcttt tatttctgta tttttccaat taatggcaga gataaattca tgtttcctga
     7921 ttcgctggat ccagatcaat gtaaaaagca ggatttgttt tttataaata tcatcttctt
     7981 gtttcaacaa cttattggaa tagatctcct gtattttttt gatttccacc aaaggaaaat
     8041 aatggagaaa gattttttga gccggtgtta attgtgcatc ttgattcata aagaagtcgg
     8101 tcattcccag attctgattg ataaaatcat agatcatcgt acggatagat cgttctgaac
     8161 ctttataaac cagtccttgc tttccataac tgacgatctc tatcccataa ctgaccagtg
     8221 ttgctcgtaa ccgtctcata tcttcatcta atgtactttt agagatttga aattcttctt
     8281 cttttttgtt tagatggatc gggattcggc tgtaagcaac ggctaaaact agatcaaaaa
     8341 tacgttcgcg ccgattcaaa aagccgtctt tcacatcttt tatttcagtt tctaatcgag
     8401 cttgcttttc cttttccagc ttcaacaaaa aacctttgtt gcgaattgta cggatctccg
     8461 gaagcttttt actgctcaac cagatattcg cttcaagaac ttcctgacgg atcgtccgct
     8521 gactgacatt gaactcactt gctagatgtt ttgcttttac tggatattgg gctgtcatga
     8581 ttctgtgaag tatttcaatt gttcttgttt tcatagaaat gatctccttc tgatttcaag
     8641 tatatggttc cgcttacata ataaaaagta gatatttctg ccacttgaag tggaaagaaa
     8701 gtaagcaaag caaacaaaaa ataagccata tgctttttca tctcctcaac gaaaaagaca
     8761 tacgcctatt attacaaaaa ttttgcccat ctatacaaaa aagaggttgt gaaaaagctg
     8821 tcctgcatca agtaataaga acaactaaac taaaatagtg cttcctattt ttcgtttggt
     8881 tgttcttatt atcgcagatg caagctttta aacaccattt agtggagagg ttgtgacaga
     8941 gatgttcagc cttgagcgat aagaagcgat tttgaaaaat cgcttcccat attttacaaa
     9001 atcgtgcctt attgccgata ggctatctct ggcacaccgt ttattcagaa aaaggagctg
     9061 cgacaaggct tttgtcacaa ctccgttcat aaatatttcc tgtcaatcca gcataatcgg
     9121 ctgaataaaa tcggcttgcc atcttttttc tttctcagaa aaatcaaaat accatatctg
     9181 agaaaaatac tgtcgttgtg tcttacttcc tgagatcaat cgcccatctt ttcgatcgac
     9241 tgaataatca cagcatctga aatcaattcg gatggcaaaa ctattttcgt ggatctgtcg
     9301 ataatttcct acttcctgaa tagttatttt tttagtatga tttcttacac cgtctgcttt
     9361 gtttttttgc agaactttct gatgttcggc aaacagcttg tctgtataac agcttctggc
     9421 tttttcagga gtttcttctt cccaagcgga ttggatcttc atgaatatct tttggatttc
     9481 cagaaactgt gctttctttt ctttagccgt tcctgacatg cgggacaata tctccgcttt
     9541 cagccttttc ttttgtcttt tattcttgac gatagaaaaa gcagaaaccc ctcctactga
     9601 caaaacagct aatgcaagaa atcccgtttg ctcataacta gaaggacctg tacctggacc
     9661 ataataccca taagaatctc tgccgtaaga acgactgcca aaataactgc ccccatatga
     9721 acgaccacca aatgaacctc tgcccgaact tcgaccgcta gatgtacctc caccatgacc
     9781 acccgcgaca gcgaatcctt gggtcggcag agcaaacaaa cataggccca ctaacaaaat
     9841 gattgctaaa taattgattt ttttcattgt cttcctcgtt atcttttatt acaaaaaact
     9901 atttttatga tagccttgct aaaaggaaga gtcaataacc agatgcataa actgtttttt
     9961 tagcaatttt gtcttattta gtcattgagt aacatcggac aaatgtatgt gtcggcagtc
    10021 cataattcct ctatcttcag catgaacaag cttgcagaca agagatctgc gcttcctcca
    10081 ggagagattt ggttttcctg acaaaagtgc aacagtgctg taaatggacg atttggtaaa
    10141 gcactttgac tagcagcatg agcaagtttt tgcatttcca ctaacgtttt ctgatcactg
    10201 cgatgcaata cgcatgtgtc atctagcgaa ctcatcaaaa ctaacaacat atgcatttgt
    10261 ttctgtacat atgaatcatt atctagctcc ttagctgctt gaattgcaag cctgatatgc
    10321 ggatacccta atatcgcttc ttcatatgca ccatttacgc catatttttg cttcacccta
    10381 tgaccatgag tggttttttt atgacgatat tttgtatcag gaaaagcagc aagcttgctg
    10441 acatcatcaa gtattttcga taaagaaaaa ggctgttcca tactgatttt ttgtgcaatg
    10501 acactgatca atagccccat tgcccaaatc gctcctttat gggtattgat gccatttgta
    10561 gcaatcagca ttgccttttc cgcttttcgc ccaatctcag caattctttc tcttaactcc
    10621 tgatcaatag catgatgcca tgctatttga gccatttctt caaaaaaagg agttagcgtc
    10681 ttagcagact taagaaataa agcatagtcc atatcggaat gtgcacccgc atccagtgca
    10741 tccactaatc caggttttgg acttaatgtt acttctgcga tcaatgcttc ctctaccaac
    10801 gcagcgagtt gctgagtcac tgcttttttt cgttcgtcat ttatcatgac actcactcct
    10861 tcttcctact attacttgct gaaacctttt ttgatagcta cccaaatgat atgaagtaac
    10921 cagcttaaag caaatgcaca acttgccgtc attagataag ctacaagaat catgactcct
    10981 gaaggtaact gcagccaact atctttaaac aataataaca gtacttggaa aacaaataca
    11041 ttggctaaat acgtacgata agcataaaca gacaaccatt tgaacaatgg caattttggc
    11101 gaatcgttta caatcatttt ggaagcgcct atgaagacag caaagatgat gactaaactg
    11161 tataaacttt ggatcgtatt caagtaaggt gcattcgcaa agctcaagtc tcctggatgt
    11221 gcgagcaatg aacggacaga aaagaacgcg ataactaagg caacaggtaa gaaaagataa
    11281 cgtactctgt ccaaatattg atagactttt tcgtgataaa caaatgcggc taccccataa
    11341 atcatgaaac tagcaacaaa ccgatctaat aagtaccagc tgtcatgata aggcccagtc
    11401 aatacttgtg tttgatagaa cagataccat gcgacatata aaatcgttga accaatcaat
    11461 actggccata catatttttt attagcaaat actttgtaac cgagccaaac aaaaaacggc
    11521 atgaacaatt ggatctgcag catcataacg gtataccata aatgaggcgc tccatcacct
    11581 gtgacatatt tcagtaaaaa gcttgcagta tccgtatacg gagtttgttg ttggactgct
    11641 ggaaatagcc aaagataagc cgtcgtccat agaagatacg gaacgactag ttcaaaaaac
    11701 ttttctttca aaaattccca ataagacaaa tgctgacttg tttttaccat attataaaca
    11761 attgcaaaaa taaacattgg tgctgtgtat ttaacgatga cgtaaaaggt accaagaaaa
    11821 gctgtatttg ttgcgctgtg gctgtttgcc aaagcaaagc tcaatatcgt ttgcatcata
    11881 acagccgtta cagctgatac tttctggaag tcacttacgt tgattttgat ttttttgttt
    11941 tcaaacatct ctttctctct ctttttcttt attaccaaag ttctttggtt aatttaggtc
    12001 cttcgactgt tttcaataga atttttttct ctggctctct caaatactct tttagtgaaa
    12061 aaccgccttg ccgagtcact acttgggtat cgaattgctc aaaaaaagca ggatgcgatt
    12121 gaattacctg aattggcaat tcaatcggtg aatttgcgta cagcagcaaa tcaaaatcac
    12181 tggtttcttt tactgccgga atcccagtcg ataattcaaa tcctaagctg ccgccgattc
    12241 cccatttaca tcctgataat atgtttctag cagcatgaaa ttgctgataa acaggataat
    12301 ccatgatttt ctcttgtcta tacgattctt gttcgatgat gtcccacgga tgaatgactt
    12361 gtttgacggc atgttttgag atttctgttg accatctctg attgcgggca ggtccacgta
    12421 aaccaatcgg tagtatttct gcgtgatccg tcgacgcacg acgaactgtt ccgaacatct
    12481 tttcagttag aacgacccat tcaggcaagg tgaacccttc tatttcatgt ttatcaaaaa
    12541 ggacaatatc atgtgcttga atcatttttt atgcatccca ttcttcgtcc attttggcac
    12601 ggactttatt tgtttcgaca cgtccgaatg ttttggctgt ttcgtttgtg tatcgatttg
    12661 tcagcatcgt gtcatttgta ttgcggacat cttgaatggc ttcattgatt gcttcgatga
    12721 ctttgctgac attttgttct gaagcagctt ggtcggtgat atcttctagg aaatgataga
    12781 tcgctcctag ttttgtataa ttattgatat cgtaagcgat agctggaact ttttcagcag
    12841 ctgcttcgat ttcagcaacc gaacgttttg tcacacgtgc agcactttct ttagacatcg
    12901 cttggacggt aatttcttta ttgtttaaag caatcaagcg gcttgattgt aatccatgtg
    12961 ctaaaaatgc acctgaaata gcatttcctg caatcaatcc aataactgga tgtccagctt
    13021 gacggagttt tgcataagca gcagcactat tagccaaagc cacatggata ccgatcaatt
    13081 cttcgttata gccgtatgct tggcttggta catcgatcac aacgatgatt ggccgtttca
    13141 cttttttgtc tttatcttct tcgtaaacgt gatccaaaat ttgtcccatg cggaaacctt
    13201 caactaaacc aacttcaccg tttcgtacac ggtacatcgg gtttttagga tcaggtacga
    13261 ttgatgtcac gatggcttct tctatgaatt tacttgattt accataatac acagtactaa
    13321 tagaagaagt tggatttttc atgccagtta gtgccgcaaa ccaatcgtaa ccaacacttg
    13381 ctgcagctgg ttcatttcct tctgctactt ctggtaagtc aattgatttt gcttgatggt
    13441 tagcaattgc ttcattgtaa tcgtcaatgg ccattggttt tgaaagatct aaagaatcaa
    13501 gtaatgataa ataaaatgct gagctttcac tgcgatgcgt gtcttttcgt tcgtccattg
    13561 ctgcgatgac agcagctttg attgcttcta cactatcagt cactaattca tcaatgattt
    13621 ttgattctac ccgttgacga gatcctaatg tattccaaat caagtctttg tcactagaat
    13681 caaattctcg aacgcctgct tcttgttcaa taacttctgg tccattcaaa ccgactctcg
    13741 ctttttttgt tgcgattaaa taactcatta atgctgaagt aatcgacata ccgccaaatg
    13801 atcctacacg tcctggaatc aaaccgatga ctggtacata ttgttttaga gcaatgatta
    13861 catttccgat ttctgaaata gataacagtc cgtagtttgc ttcttgcaaa cgaacaccac
    13921 ctgtatctag tacaatgact ggaaagatct catttccttt tttattttct tctaacacat
    13981 gttcaagtgc agcaacgatt tttgcaccgg aaacttcacc gataccgcca ccttggaatt
    14041 ttccttccat agcgattact actgcttgtt tgccattgat cgtgccacgg ctgacaacga
    14101 tcccgtcatc actttcaggg acgattcctt gtgctggtaa acttggcgcg atgatttgat
    14161 cgaatggatc aatcaattca cgtccattgt caagtagagc gttcactcgt tctctggcgt
    14221 ttaattctac aaaactattt ttcatctgaa agttcctcca ttgcttgcgt caatcgtagg
    14281 tacacaactc ctggtgtagc accgaagtca ttgatcacga tatctgctga aattggataa
    14341 cgatcaaaaa atctcgttaa tacattgttc catacttcgc cgaagccatc gcttcctgta
    14401 caaattttta cagtagattc tgttttttct gttggaaaca tcaagatttc caaatctcct
    14461 gaaccgacca ctcctacatg gattttctta ttgatcggct gtttcgttgg atatttataa
    14521 gctaattgtt ccatggatct ctcccctttt tgtaaaacag agcatacacg ttctgcttat
    14581 tcgtctttct tcttccatct gggtaaatgg cgacaaaagg tttttctcgc caattttatg
    14641 accaagttct gaatttcatt ggtggttcat acaggccatc tgaccattcg atcaaatcat
    14701 cgatggtttg tgcagctaat aatgaacgtt tcgcttcact acgtttgatg cctagatctt
    14761 ctggtaattt cacgatgccg gctgcacgca agctatctag tgattctttc gtgctagtca
    14821 ttccaagagg tgtaacaccg ccaattgctg caattgctgc ttgtcgttct tcttttgttt
    14881 ttgctttgta caaataagca attccttctt ctgtcacgat atgtgtagta tcttcgctat
    14941 aaatcatcac tggtgcattg gctaagcctg cttgtttttg aacagctagt gcatctaatt
    15001 cttcgacaaa gactggtttt ttatttgaac catatgtttc aaccatttga acaactaatt
    15061 tttggccttt ccctaatgga tcatcattgt cgcgaagtga ttgccatgct ggtgtagaat
    15121 gacgacgacc gccaggatta tgtcccatgt taggcgcacc gccaaaacca gataaacgtc
    15181 cttttgttac ggtagaagag tttccttcgt aatctaattg caaggttgaa ccgataaaca
    15241 tatcgatggc atattgtcct gcagcttgtg ataatgttcg gttactgcgc atcgtgccgt
    15301 ctttaccgac aaaaaagaca tctgggcgag cagcaatata tttttccata ccaacttcgc
    15361 caccaaaact atggatgctt tcaacccaac ctgattcgat ggctggaatc atcgttggat
    15421 gtgggttcaa tacccagttc ttcgcgattt tcccttttaa ccctagtgat tcaccataag
    15481 ttggcagtaa caattcgata gctgctgtat tgaatccaat accgtgattc aaagattgta
    15541 cgccgtgttc tgcgtagatt cctttgattg ccatcattgc cattagaatt tgcaattctg
    15601 tgatgttttg aggatctcgt gtgaataaag cttctaattg ataaggttta tctgctttca
    15661 cgacaacgtc tacccagtca ccagggatat ctacacgtgg taaagaatcg acaacttcgt
    15721 taacttgaac gatcacgata ccatctttga aagctgcagc ttcgatgatt gttggtgttt
    15781 cttccgtatt gaaacctgtg tacaagttgc cgtctttatc tgctttatct gcagctacta
    15841 aaacaacatt tggaatcaaa tcgatgaata agcgtccata aagttccaaa taagtatgga
    15901 tatcacctaa tttcattgtg ccgtcttcaa tcatttgggc gacacgcaag ctttgtgcac
    15961 cagcataaga gaaatcgatc ttgcttgcga ttcctttttc aaaaatattc aaatgttctg
    16021 gtcgtgagat actagacatg atcaaatgca gatcattcac tttttctgca tctacttttt
    16081 caagactttc tgaaagaaaa cttgcttgtt tttgattgtc gccttctaat acaactttgt
    16141 cgccgggagc aatcagtttt tccattactt ctacgatttt ttcagtttct acatacttgc
    16201 cattgagcaa gttgctgatt ttttccaatc gctgcgcttt tttttcgcgt ttagaagccc
    16261 agttgcggtt tggtttttcc ataattagtc atctccattt ttgatataga aacgtggcgt
    16321 catcaatacc atgttggtct agattgatcg tacggatccc tttatctaca ccaaattttg
    16381 tatgaatcag ctttgtcaaa gtattgccgg ggggaaattc aatcgtcaga tccattccta
    16441 attcttttgc caagtccatc atttggttcc actgcacagg gaaagctagg ttttccaaca
    16501 aatctttacg aatctcttct gcgtcctgag tggctcttcc atccgtattt tttatataca
    16561 aacatctagc atcagataga tggacagact gcatatacgg cttgaatttc ttgaccgtat
    16621 cattcatcaa gagacagtga gatggcacag ggactttcag cagcttcgct gtttgcgctt
    16681 ggtgccgttt ggcttctgcc aacgtaacgg ataaaccttc gatactccca gataacgtat
    16741 gttgcatcgg acaattttca tttgacagat aaactggatg gtcaggatca aatgtttttg
    16801 ctacaatttc ctcagcttcg cttctcgtca cacctactac tacgcccatt gcataaccgc
    16861 ttggataagc ttctttcatt aagactgcgc ggtgatagac tagttccgct gcattctcaa
    16921 atctcaaaga tcgacaagca acagctgcac aaaaagcacc aatcgaatgt cctgctgcaa
    16981 tatcaggctg aataccgttt ttttccattt catctaaata aaatactgct tttgtcagta
    17041 atgctagctg gataaaaacc gtatcttgat agttttcttc tttatcaact aaccgatacc
    17101 ccgtcacatt ttctaaacgt tccatgtaca tttcagggat atccgctaac atccctaact
    17161 tctgactgcc ttgtccagga aataagaaac ctgtactcat gagcctcgcc tcctcagcac
    17221 ttactttttc cactgctatc ttagtcaatt tctgctataa taaaaaatgc caaaaaaaga
    17281 aaaaaccata acaaaaaggt atggatggga tggataatta tgaaattgct tcatctgcag
    17341 tattttaaaa aagtattcga aacaaaaaat gttacccaag cagccaaaga attatttatt
    17401 tctcaacctg ccttgagccg tgcgatcaaa catttggaaa acgaattagg tgtccctttg
    17461 ttttaccata gcggcagaaa cattgagacg accgtttatg cagaagaatt ttatccatat
    17521 gcagtcaaga cattagacac attagaagaa ggaatcaaat tattgtcttc ggtcaatgag
    17581 aagatcgtta cttccgtcat attgtattta gaggtagcct ctgtttctat tccgaacttg
    17641 gtccgaattt ttatggaaaa gcatcctgat atccaattaa cgatcatgca acatcattta
    17701 ccagatgata ttaccgatcc tgtactgtac atcacttcgg agatgaagcc tggcatgacc
    17761 aatattccta tcatgaaaga acctgtttat gtcgccattc ctaagaaaca tcctcttgct
    17821 aaaaaagaac atctgcaatt agaagatatc caacgcaccc ccatcttgat gctgtcaaag
    17881 agcaacgcct tccgtcatac actggatgca gcacttgaag cgaaaagtat cgaattgacg
    17941 atcagttcga cgacagatga tcctgcgaca ttacgttcta ttgtcaagca aggattagct
    18001 gttagctttt tcccaaaagt ttcttggtct tacgataaaa atgatccttt cgtcttgcgg
    18061 gaaatcaaag atttgccctt gacacgaaca atttatttat cttctagctt cacagaagat
    18121 catcctttga tcaaaatgat tgcaaaaacg atgcgggaat tttatttagg aaaataaaaa
    18181 aaaaagagct gggacaaaat tcttgtcgca gctctttttt cgaacaaacg gtgttccaga
    18241 agtaatttct tagaaaataa gccgaaattt cccaaaaatt tgagaagcaa ttttcgtaaa
    18301 ttccttctta tttcttgaag ctaaacattt ctgtcacaac ctcttctcta ttgtcctaga
    18361 cattcattgc gttgatttaa aacttccatc aatcaaatct gctagttcat ctagcttttc
    18421 tattttacgt agatcaatag agatccaatg ttctttattc atgtgatagg ctggaaaata
    18481 cccctctttt cctcgcaaaa ttccgccaag ttcaggatcg accttcaaat caagaatatt
    18541 gatgatccta tggtcacttg catcatcata gattttttct tctggaacag acatcaggac
    18601 cccaaaccat tttttgcttt tttgatgtct gaatacgcaa taatcaggaa attttttaaa
    18661 aatatattct ggagacaaat tgtatgtttc attgatgtga tcgattagtt cttttttcgt
    18721 tagcatctgc ttccatctct ttcttattga ttcgctttat tcaaagatac cacgtaagaa
    18781 tagaaaaatg aatacaagta gttgctaatc aaatacttcg atatttttct tttttatcaa
    18841 ctcttaaatg ttcaatccgc ctgtgttggt cttgctacca tctactatta aaatgtttat
    18901 tattgtttgc ccacctattt tttg
//
LOCUS       DAYYHX010000040        18656 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000040 DAYYHX010000000
VERSION     DAYYHX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.040"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..279
                     /locus_tag="ACHA6Y_001010"
                     /pseudo
     CDS             151..279
                     /locus_tag="ACHA6Y_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
     gene            393..632
                     /locus_tag="ACHA6Y_001011"
     CDS             393..632
                     /locus_tag="ACHA6Y_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987123.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            744..938
                     /locus_tag="ACHA6Y_001012"
     CDS             744..938
                     /locus_tag="ACHA6Y_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987124.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            1024..1695
                     /locus_tag="ACHA6Y_001013"
     CDS             1024..1695
                     /locus_tag="ACHA6Y_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8987125.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1748..3724
                     /locus_tag="ACHA6Y_001014"
     CDS             1748..3724
                     /locus_tag="ACHA6Y_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8987126.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3739..4491
                     /locus_tag="ACHA6Y_001015"
     CDS             3739..4491
                     /locus_tag="ACHA6Y_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8987127.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4507..5262
                     /locus_tag="ACHA6Y_001016"
     CDS             4507..5262
                     /locus_tag="ACHA6Y_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8987128.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            5275..5535
                     /locus_tag="ACHA6Y_001017"
     CDS             5275..5535
                     /locus_tag="ACHA6Y_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987129.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5532..7622
                     /locus_tag="ACHA6Y_001018"
     CDS             5532..7622
                     /locus_tag="ACHA6Y_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFM8987130.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7649..8113
                     /locus_tag="ACHA6Y_001019"
     CDS             7649..8113
                     /locus_tag="ACHA6Y_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987131.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            8110..8352
                     /locus_tag="ACHA6Y_001020"
     CDS             8110..8352
                     /locus_tag="ACHA6Y_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFM8987132.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            8370..8672
                     /locus_tag="ACHA6Y_001021"
     CDS             8370..8672
                     /locus_tag="ACHA6Y_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987133.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8742..10805
                     /locus_tag="ACHA6Y_001022"
     CDS             8742..10805
                     /locus_tag="ACHA6Y_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987134.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10805..13417
                     /locus_tag="ACHA6Y_001023"
     CDS             10805..13417
                     /locus_tag="ACHA6Y_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8987135.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            13414..15945
                     /locus_tag="ACHA6Y_001024"
     CDS             13414..15945
                     /locus_tag="ACHA6Y_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM8987136.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            15995..16327
                     /locus_tag="ACHA6Y_001025"
     CDS             15995..16327
                     /locus_tag="ACHA6Y_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987137.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            16328..16921
                     /locus_tag="ACHA6Y_001026"
     CDS             16328..16921
                     /locus_tag="ACHA6Y_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987138.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            17084..>18656
                     /locus_tag="ACHA6Y_001027"
     CDS             17084..>18656
                     /locus_tag="ACHA6Y_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFM8987139.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQR"
BASE COUNT         6891 a         2925 c         3837 g         5003 t
ORIGIN      
        1 gatcaaataa acaagaaccg taggaactac ggggatagct tggtaaataa gaggcacctc
       61 tgttagtaaa gtaatacgct atcaagtatg ctctcttccc aagaagctcc cacttcaagc
      121 gtaagaaacc gttaggttta gctaagtggt gagtagttca caatgacgct tacgaatgat
      181 aatggagacg aattatctgt tgaagatact tggtcatatt tgagtgatta tattgtcagc
      241 gttgaaatca ttgaatttgt agcggataaa gaagaataaa aactggagga ataaatttta
      301 cagataaagg cttagaagaa gtcacgacga ataataaaaa ggtagtatta ccttaggcat
      361 tgagtaagga attcatttag gaggaaaatt agatggcgcg accagtttta gaggcgatgg
      421 atatggatac tttgacaaaa ggaaggtatg tacagctgtt agctcgtggt tattcaccta
      481 aagatgtatt caaatccctg tctaaaggaa cagatatgga aaaagaaaga ttacgaaaag
      541 aattcgatta ttggagaacc cataatggaa tcaaggattt gaaaccagca cggccagtaa
      601 ttaaacgaaa gaaaaagata acccaagact gaagtgtaga gcgtatgtga tcataggctt
      661 ttttattttt gcaaaaaaca aggaaggtag gaaaactcat gaattatgaa gaaatcgaaa
      721 acagaaaaaa agtgtcgaaa taaatggaag aaaagttgct aaaaacgatg aaacaaaaac
      781 accttaaaag attatctgtc atgcaatata tcaatgatat gcagataaca ggaaaagaga
      841 aagcttgttt gttaggttca atgaaaaact ttgaacagtt gagacgaact tatgtgaaaa
      901 caagttctaa ttgtcaacta ttgttagaag tctcatgaca ttcattaata gaattcattt
      961 ttaaaacttt ttctatggaa atgagaaaaa gattttttgt tttgtctgaa agggggtagc
     1021 tggatgagaa aatggttatt tggtttttta ggagtcgctt taatcgtctt ttgcagtgtg
     1081 tttggctatg ttagctacca aaaacatgaa ggagaggtgt ttaagcaaaa tatagaaaaa
     1141 aagatgcccg tagatcaaat caatgcacat gcaaagagtt ataaggagga tgcaacgaac
     1201 gtcaataatg atatgtcttt ggggcaaatg ttgtccatac aacaagaagc aatcgaaatg
     1261 ggcgtcaata aacaagtatt tgcacaaatt cagattcctg cacttggttt agcattgcca
     1321 atttttaaag gggcaaatca atacactttg agtcttggag cggctactta tttttatgaa
     1381 gatgctgaaa tgggcaaagg aaactatgtc ctagcaggtc ataacatgga aatgccaggt
     1441 gttttatttt ctgatattca aaagttgtca ttaggggagg tgatggactt agttagtaac
     1501 gatggcgtct atcgttacaa ggtaacgcgt aaatttatcg tacctgagta ctttaaatta
     1561 atcgatggcg ttcctgaaga gaactctttc ttgtctttgc caaagaaagg agagaaaccc
     1621 ttattaacgt tgtttacttg tgtttatacg tcacaaggaa aagaaagata cgtggtacag
     1681 ggagaactac aataacaagg ttttaatcaa cgtgagtcgt tgaaatatga aatgaggaga
     1741 gtaaaaaatg gcaataaatc ggtatgtaaa aaaagctgga atgcttattg gaatgttagg
     1801 aatcattgca ggctgctttc agggatatcg agcatatgca gaagatgtca cgcaaaaaac
     1861 gccaccagag aaggtcaata tcacggttca taaattaatg tacgatcaag gaacgcaatt
     1921 gaatgttgat attgacggga tcaaaaacga tggttatacg cacgatcaat atcctacagg
     1981 tgtaacgaaa tacaacaagg cagattatgg tgatgttgag tttacgttag gaaatattac
     2041 ggatcaagta ttaccgagag aagattctga tctaaccaac gcgaaagtgg acgagattgt
     2101 acaagatgta gaagacaagg gatctaacag tgagtacgtc aagaatgcaa ccaacaaaat
     2161 tacttcagct gtagatgaaa atggtgagat cacttttact gatcaaccag cctatgtcaa
     2221 ttccaaaggg aatgtatatg tggtttatga aagtaaatcc gctgctggat tagtcacaca
     2281 aaaagcaaaa ccaatggttg tgattgcgcc aatgacagat aaaacaggat caggattttt
     2341 aaaaaatatt catttgtatc ctaagaacat tgtttctaag ttgagttttg aattaacgaa
     2401 atttggtgac gatggcgcag accaaagcaa acaaaccctt ttaaaagggg caaaatttga
     2461 gttatacaag ggcgaacctg gaaaaggaac aaaattgggt gatcttgtat cggatgatca
     2521 aggaaagctg actgcgactg atttgacgtt aggaaaatac tattttgtgg aagttccttc
     2581 ggaagttgta gtgggatcag atcaagaacc aacagcagat caatatttat taggcgcaga
     2641 cgctcgcaac gatgcgcaca ataaattaac ctttgaaatt acgaatgatg gcgtgacaag
     2701 tgacttaaaa gcttcatacg taaactataa agcaccagtg atcgataaaa cagtaacaaa
     2761 tggaacagga caagaacatt ctttccaaat tggtgacgca gtaaattatc aaggaacgat
     2821 tcatattcca acagacattg caggtggcgc agacggtatc acagtaaacg gtgtgaaaag
     2881 tgaaacttct ccttacagtg tatttaaatg gggagataca gcaggacagg gactttctta
     2941 tgtcgctgct aaagcaaata tcaaagtaac aaataaagat ggatctgtgg tattaaaaga
     3001 aaatacagat tataaaatcc aaaattcaga aaatggcttt gtgatcgatt ttatcgtgaa
     3061 taatggccaa gtaagtgata ctgtagcaag tttacacgga caagatcttc aaatgactta
     3121 taacatgtat gtaaatgaca gcgcagctgt tgccaatcca ttaacaaata gcgtggattt
     3181 cgtttataac aacaatccat ttaatcagga agaacaccac gaaaaaacga aagctgacgt
     3241 tgtgacttat ggtgcaaaat tcttaaaagt cgatagcgga ctatttggaa caggaatcaa
     3301 agcaacaccg cttgaaggcg cagaatttgc tgctaaaaac gcagaaggaa aatattatgg
     3361 tggattagtt gatacggata aagacggtgt caaagaagca gtatgggtag acgatgtagc
     3421 aaatgcagcg atcctaaaat cagataaaga aggtcacttt gaaatcactg gtttaactga
     3481 aggtgaatat tcactggaag aaacaaaagc gcctgaaaac tatcaaaaat taacgaaaga
     3541 gatctctttc aaagtagaca aagattcata caaagaagaa aaccgaatca cgatcaaaaa
     3601 caatcaaaaa gcttccgtac caatgaccgg tagcaacggt tttcaaacgt atgtattaat
     3661 ctcttgcttg ttgctaggag caggggcact ttctgcagtt gtttacttta agaagaaagc
     3721 ctagaaggat caaaaccaat gattatcagg aagcctaaga aaaagcgaat catgggtaaa
     3781 tggatcattg ctttctggtt actttcagct gtaggtgtct tacttttgat gcctgctgaa
     3841 gcgagcgtgg cgaaatacca acaaaatcag cagattgccg caatcgatcg aacaggaacg
     3901 gcagcagaaa cggattcttc tttggatgtt gctaagattg agttaggcga tcctgttggt
     3961 attcttacaa ttctaagtat ttctttaaaa ctaccaattt atgacggaac gtccgacaaa
     4021 attttagaaa atggtgtagg tattacggaa ggaactggtg atattacagg aggaaacggt
     4081 aaaaatcctt taatcgcagg tcatagtgga ctttataaag ataatctttt tgatgattta
     4141 ccttcagtaa aaaagggaga aaagttttat atcaaagttg atggtgagca gcacgcttat
     4201 caaatcgatc ggattgagga agtgcaaaaa gacgaactac aaagaaactt tgtcacttac
     4261 ttagaatcaa atcccaatga agatcgagta acgctaatga catgtacgcc taaggggatc
     4321 aacacacacc gttttctagt gtatgggaag cgagtaacct ttacaaaatc agaattaaaa
     4381 gatgaagaaa ataagaaaca aaaattatct tggaaatggc ttttaggaag tactgttttt
     4441 ctttcagtca tgatcattgg ttcgctattt gtttataaaa agaaaaaata aaggagggta
     4501 accaacatga gaaatagaaa atggctattt gtttgtgcag ccgctctttt ttctttcact
     4561 gcctacacac agttgacgtt tgcagacaga caagaaagca cggatagcac agaacaaaca
     4621 atccaaacgt cagatagcag cacgattcaa agcagcgatg aaaccgtcga ttcgtcaaca
     4681 gttgattctt cagaagaacc taaaattgct gaagacgatc aaatagtcaa acagaccatt
     4741 cacgttcaaa agattatttc ttcagaacaa ttagacaacc aggggcatga aaaacaagaa
     4801 ggagtaaatg gcgcgaagtt ttctgtttat gatgtatcgg acattttaca aaagatggat
     4861 gttaaggatc tcactactga ccaaattgaa tcccaattaa aaaatcgagt gaaaaaacta
     4921 tccagtgatc aattgaagct agtttctaat gaagaaacaa agacaattga tcaacaaaca
     4981 ggtgtctttg aattctcggt agaagtacag gcaaatcaga aacaagctta ttatattgtc
     5041 aatgaatcaa gtcctgaaaa catttctaat agcgaggata ttttgctatt aacacctgtt
     5101 tctgataaaa acggtgaatt cttaaaggat gtgtggatct atcctaaatc agaagcttcg
     5161 caacctaaag aagaagtaaa aaaaatcgtt tctactggcg taaagaaaaa cttctttgag
     5221 aattgttggg attttgttac tcacttgttt tctagagatt aaggaagaaa taaaatgaaa
     5281 ctgatcaaat tattattatt caatttttct tgtttaaccg cagtcttttt gtactgcggt
     5341 ttattatttt ctaaactatc tattttgcca agctaccaac tatacctaat gaggatttta
     5401 aacataccag ctgcttccat gccgctagat attatggaaa agctcattag gaatccatta
     5461 tttactacga tcaatttgat cgttattgtc ggaatttcac tagtcattta taaaaaagag
     5521 gggatgaagg gatgaatttt aaacactcgt ggacaagacg gttaaaatat gtttttccca
     5581 taatgatgat gttaggtaca ttgtttgggt taaaaacagc caatgtcttt gctgaaaaag
     5641 ttattgtcga tccgtcaaat ccaatccaga acgtaaagaa taattttatt attccaaaag
     5701 atgttcctaa tgcaaaaaca aatattactg taaacggagc gtcaaaagtt tacaaatatc
     5761 atgttgatgc caacaaaggt ggttttacag acgactacgt aggattagtg gagggaaaca
     5821 aaaatatacg ctatccttct ttccaaaaag aatacggtga aacaaacctt gttttagaag
     5881 gcgtaacaac caacaccaaa gtcaatattg actatggaaa aatcggtacg tataacggga
     5941 aaaaagtcaa tatcaagcta gttctatcta atattcactt atactcggat acgttgccgt
     6001 ggaatctact agataacaac tatacaaaaa ctcattttcg agacgatggc tataaaaata
     6061 cgaatggtgc aatgagtaaa tctaaaaaaa gaacagtact ttggatctca gacaatcttt
     6121 tctcaggaat tgtttaccat tcaacacaaa tgaatgtgca gctagtagcc acatatgagg
     6181 acggtagccc cgttcaattt tcaggagata catttatcag ttttaactct ttgaaccctg
     6241 caggaggaaa aagcacggat cttaaaggag aatatgccca ctatgacaaa atgaatacga
     6301 cagattggta tgtcagaaaa gatacggttc tttcagaatt taaatctttc tacaacaatc
     6361 tgaacgtggt aggtggccac ccaggaggaa gctctaagct gacacaagcg gataatgatt
     6421 tcaataattt gcatgataaa ttaggagatc cgaagtttgg tcaaggtact gtgtccttta
     6481 aaatatctga agcaaatccg acgtttgtga ttggttcttc taatgttcaa acctggttta
     6541 ctttatcttc tgcgacaatc tttagtgttg ttcctgatca acctgaaaaa acaggggttg
     6601 ataaaaatgg aaataacgtg aacgataaaa tgttgcaagt aggagacacg attcaatatc
     6661 gaatcaaaca gaaggtgaat cgattaggag tggatttact agcggtttac gatcgatttg
     6721 agttaatcga taatttacca aaagaagtaa attacgtcga tgcacacgtt gaaagtggta
     6781 caaataaaaa attcgatgta tctggtgaag tgacctatga caaaacaaaa caccaggtaa
     6841 aatatgcagc gaaagctgat acattaaaaa atcgaatgaa gtataacggc gaaacttacg
     6901 aattagtgat caatgtaaaa gtcaatgaat tggccaatca aaatagtgta gccaaaaatc
     6961 agggaacgtc gatcattaat aaagtagaaa aggatacaaa tatcattacc gtttatttcc
     7021 ctaaaattcc agtgaaagaa gtacaacaaa atggaaaaga tgtaaacgga cgaaatgacg
     7081 gtaagaaggg tacgccgaca gctcctttaa acgcaggatc tgaagtacaa tatctagtca
     7141 cacaaaaatg gcacacaaaa ggcgtagacg ctgcgtcaga tcactacaaa caatttagta
     7201 ttcaagatcc aattgaagct cgtttgacat ataaagaagg ttctgcgcaa gtaatagata
     7261 agagtaaagg aaaagatatt acttctgaag gcacactaac ctatgatagc aattcaagaa
     7321 cgctgaagtg ggaagcttcc gctgacttct taagtaacaa tcttttagat ggacgagaaa
     7381 ttcagctgat atttacagct aaaactccat tacaatcaga aaaaaatatt gataaccaag
     7441 ccgtagtagc agtagataat gtttctaata aaacaaatgt tgtgacaatt ggggttgatc
     7501 ctaacttgcc acaggtcatt gttcctaaaa caggttctac acatttagta acgatttcag
     7561 cggtcagctt actattactt gtgttagcaa cttttagtta tgcggttctg agatacattt
     7621 aaaataaaaa aacgaaagaa tgaataaaat gaaaaagtta ataatagtag ttacttcctt
     7681 gattgccatg attgttattt tagtcggatg tagtaatgaa aaaaataaac aaactaatca
     7741 agataatggc gtattgaaat caggaactat gtggaaagaa gaagtaggtg gtcttgttta
     7801 taatttgaaa attatagacg agactacctg ggaatactct gagagtgtat ggcatccaga
     7861 tcctgtacaa ataacagtca agaggcaaaa agactataaa ggtttagaaa gatacaaaat
     7921 agtagattca gctggtgtta gggaatttat aaacaaatct gattctttat ttattgttgt
     7981 tccctatgag aagaatggcg taaaaaaaat aatttttctt ggatctagca aggatgaaaa
     8041 gcgaacgaaa gagaaattga ttcacgatgg atcacaatcc aataaatata agttacaaaa
     8101 gactagcgag tgattgctag tctttttatt tgtcctgaag gaggaatcac agtggcaaaa
     8161 gctaaaacgc catttgaaaa agccgcgtct aaaatcttag aacgccgcgg tgtatctgaa
     8221 acacagtgga aacaagaagt ccttgaaaaa gcacaatttg acctattacg gggaaaagac
     8281 aaggacttag aaggatatat ctatcaacgt gaacgtgaaa agcttgtttt cgatgaaatt
     8341 attaaacact aaaaggagcg aacaatttta tgtttgaatc aattacaatg aattttttaa
     8401 cacaaccgaa agtagtagct gcggttgata tttttagagc attacaaact gccgcaacta
     8461 gtttaaaaaa caatctgatg atttttgctg ctgctgcggc cgttgcttcc attgttattg
     8521 ggggtctttt cttcctattt ggacgaagag gagctgaaaa tgggaagatg cacgtaggct
     8581 atgtattgat gggggtagga gttattttag cagcagcttc tattgtggtt tcgttatttg
     8641 gattgtttgg acaaacacct caaggattat aagaaagtaa aaaagtagga gtaagaagta
     8701 gtcgatactt tttactcctt ttttatgaaa ggagaatact catgtacttt acgattgcag
     8761 gcaataccaa aacagataaa gagttaataa aaaaagcaac gaaggaagta ggaaagcgta
     8821 cttctttttt accgaaaaca attaaatatt ctgtcattga aacggaagaa cagctacatg
     8881 tgttagggaa agtactggaa cacgaaagtc gatcttctgt taagttgact ctcacgttac
     8941 atgacacaga aggaaaaata gagaaagctg gtacagaata cgtttttggc ccactggatt
     9001 atcaaatttc agaaggtttc tatcctacat tgatggattt aatccgcctt gatgtgttta
     9061 aagaaacgga tgaaactatg agtgtggaag agtcttatcg aaacgaaaaa cttttacaag
     9121 ttttagaaga agagttagag agcgaccaaa gaaaagtgaa tcaagggagt gaagtcgaag
     9181 taaatgaaga agtaccatgg gaacgaccaa tatttccaaa gaaggagtca aaagaaaaag
     9241 agacagtcta tgttgacgaa caagctgata ttccgactca gaaatcgata tttgaggaag
     9301 agaacgatgt gatgggtcaa ttggaggtag atccctttga agaaacaatg gaggatgaag
     9361 agacggccga agtagaagtt gatccagttg aagcgaatga agagtcaaca tggactgaag
     9421 gggattctca ggaatatatt gaaccactta tcagtgaaga agtagaaaaa aacgaaatag
     9481 agatacaaaa ggcaactgta tttgattata ttttggatca atatccgcca gagcatgaat
     9541 ggatgaaaga aaaaaacaaa ttatttttaa atgagcttta ttcgaaatat caattacccc
     9601 aaacctatca agcattttta gagagtaaag aagaattaat ctcacagggt aaaggcacac
     9661 ttttagaaca gttagagtct gtccaatcaa caaattggca tgagcttgca caagaagatt
     9721 tattagccac ctttgaagaa cgtcaacaag aaacagatca agaaattaat gagtatgcga
     9781 cggaacagtc gtctaattgg gagaaagcaa aagaggaact aaataaagaa gaacagcgag
     9841 tgattgaaga agaagtacaa aaaattaaag agcgatacga aaagaaacgc gacaatgcgt
     9901 attccttgtg tgtagcaaag atcaataatt ttactcagac gaatcaagaa gcgttatcga
     9961 aagaaaaaga aaacttgtta aaagaacgta ttaaagaacg aaaagaagaa ttttaccgag
    10021 gtttgcgtac agataagcta aagattagca aagaattaaa tgaacaactg aataagctat
    10081 atcaaacaac gactgaagtt cttatggaaa aatacgaaaa gatccagcaa gaattacaag
    10141 tgcaaattcc aaaatggaaa aaagagtatg aaattcaaca acaagaattg aaagaacaag
    10201 aagaaaaaca acggcaaaaa gaaaaagagc gcgaacaatt agaactaaaa aaacgtgaac
    10261 aggatcttgc ggaacaacaa atggcaatag aaaaacaacg gttaaaccaa gaaaaggaaa
    10321 aagaagcagc tgctcgtaag gaacgtcaag aacaaatgga tacattgcga caagctcagt
    10381 taaattatct agcacaacct caaccaacgc cttcagtagc tgctgttcaa accccaatga
    10441 acaaccaaac aaatctaact ccttcaccac agcgttcctt taacggatgg atggtgggat
    10501 gtattgcaag tttaagcttg cttctaggtg gaggaacagt atttgctttt aatcatatca
    10561 atcaatcttc acaggctgat tctgtggcca caacgacgtc aatcaaggaa gaagcccaag
    10621 aacaagcaag aaaagagtat gaacaacaat tagaagaatt attgaatcgt accaatcagt
    10681 caacaacaga gtcaagcatg gaaaatagca aagagagtga ttctagtaag gctaaagaag
    10741 gatcaagcga ttcaactgta caatcaaaca cttccgaaag ttcagaatca aatgaggtga
    10801 aataatgcgt gttagagaaa caacgaagcg aaaagacaaa cgattcaatt tagaaaagaa
    10861 taggtatctt tctcatttct atcgctatac tccctcagga gaagagctac cagaaaaaca
    10921 tgacaaacgc atttcgaaaa agatttttta tagtggactt gtatacctcc ctttagagta
    10981 tttagtaaca agagtaccag aagttttcaa ccgattggaa attgttaagg gaagcttaca
    11041 aggaataaat ctgtttcaat tacaggaaat gccacaggtc tttcattttg attcagctgg
    11101 tttttggaat ccatttactc ctacaactag tttgggtaca cgtttagcaa tttcgacagt
    11161 ttgtgtggtt ccagctgtac ttatgggatt gaaaatggac atgtggtttc gtcctctagc
    11221 agatggacaa aaaggggata atcgattaat gaccttaaaa gaaatccaac agatttatcc
    11281 gcaaatacct gaaacaaaaa ctacttttcc aggttatgga ggtattccaa tcacacatta
    11341 caaaaaatat tggtacattc aaacagatac tgttaatacg tgtattgttg gtacctctcg
    11401 atcagggaaa ggtcagattg aagtgttagc caccattgac aatttatctc gtgcagaaaa
    11461 acaaagttca atggtcgtta atgatccaaa aacagaatta tatgttggta gtaaagatga
    11521 actagaagct agagggtatg atgtatatgc ctttaatatc accgatccac tgcaatcaat
    11581 gagtgataat ccattggcac tcatcgtgaa atactggaaa cgaggagaca tagatactgc
    11641 aacacagtta accaatactt tcacaaatac gatttattat gatgccaacg cttcagataa
    11701 caaatacttt aacgaaaacg cgcagaaagc tgtcaatgcg cttatttttg cgttgttaga
    11761 acaagctgat caaacaggag attactccaa attgacaccg aataatctcg ttgagcttct
    11821 ttctgagatt ggcggtttta attattatcc agatccagac aatgaattca aacagttgaa
    11881 tgccttagat gaatttatga atcagttgcc acctggaaac attgcaaaaa aacaatatgg
    11941 ggcaacgaaa attggatctg ataaagcgaa gggaaatatt ttatcaacgg caatcacggg
    12001 attgaatcca tttactctaa cgaaaattgc aaagatgact tctcaatcaa cgattgatta
    12061 taaatcggtt gggtttccaa aatacttaga tttgaaatta catgaaagtc ttttaaatca
    12121 acgagtcgta gtagaatttt atcataaggg aaagaaattt catgaagaac gtgtcaaagt
    12181 aggctataaa ggattttgtg aaatcaattt tgattgtcat ttagttgcag gtgatcaagt
    12241 ttgtttaaaa tacgatcatc aaggaaaaga atatgtggct tggtatgaat tttctcaaag
    12301 agtattgaaa gacgaagagg gtaatatcgt ttatcggaaa aaacgaggag aaacccattt
    12361 gccagaatta gaaaaagaag ccgttctaac attagatgag gagaaaagta acttattagt
    12421 agagtctatt cggatgcact ataccgataa accaatggct atttttatgc ttacacctga
    12481 ttatgatcca tctaaccata tcattgttag tatttttctt agtcagttat ataaagaatt
    12541 atctacgcaa tgtgttaaga ctaaaggaga taaatgtcac cgcaggattc attttatttt
    12601 agatgaattt gggaatatgc caccaatgga taatatggaa ggaatcatga cggtcacagc
    12661 tggccgaaac atgttgtggg atctgtttat tcaatcttat cagcaacttt atgcgaagta
    12721 tggagaacaa aacgcagcga ttatcaaaga aaattgtcag acacatattt atattatgtc
    12781 gacagatgat aataccatcg aagaattttc taaaaaagtg gggaataaga cggttgaaca
    12841 agagaacagt accatcaatt cattaggtat gaatactcat atcaatcgca atgttgattc
    12901 agatcgtatc ttaactcctg aaagaatttc tactttttta gaaggtgaaa cgattgtact
    12961 cgctccttta aaacgacaag atttgaaagg tcataaaata cggccgtatc ctatttttaa
    13021 tcatggggaa acgaatcttc cttatgctta tcaatatatt gaagatttca atccatcgtg
    13081 ggatatcaat gatatagaca tacattgtac acatgcggac ttggatctta aggctttaca
    13141 aattgattat gattatttcc taccagatga atatgctaga aatctctatc atggactgca
    13201 tgatcaatta gaagaaaaaa acgaagaaaa tatgttttat aaaatcgttc aatctcttga
    13261 gaatgagacg gctaaaaaat tgttaattca attttacgaa gaacaagcag cagatcgact
    13321 tcaagaatat gttgaaaagt tgatcaaaaa taaagaaatt tctgaaatga ttgggaaacg
    13381 gttgttattg gaatctgaaa aggtggtgaa agaatgatca ttggaggaat tttttctggc
    13441 atagccgact ggtggtcagg cttattcgga gaaggggatg caaaccccaa aaccgtaacg
    13501 aaagtcttac aagattatgg taattatctg cagtatcaag atgtatttag ttggatttgg
    13561 aacacttttc tgtggggatt gattaaagga ttatataatc tcaatacttt actagaaaaa
    13621 accatttatc aaagctttga tttaaaagac ttattgaacg ccgctggggt aaatgcacta
    13681 tttcaaagtt tgattagtaa ggtattggct cttttttgtg tcgtcacatt aatttattta
    13741 ggcttgaaat tttttatgag taaacaccca ccaaaagtca aaaatatctt ggttaattta
    13801 tttatggcca tgatcttgat ttttggtggg agttctttaa tcgatgaagg gttaaatatc
    13861 agtaaaagct tttacggaga cgtcactgca gcaaataaag cagataaaac gggttcacct
    13921 gcttttcaaa ttattcaaaa taatgtgatc gatgtcagta cattactagc aactgacccg
    13981 aataaagtag aatccctgcc aacagacaaa agaaatgctt taacagcgaa aaattttaaa
    14041 acagcgaata tcaatgggat tattactcct aaacaagcta aagaaatggc ggataaagat
    14101 aagagtggtt tatcagattt acaaaaggat cggttaagag cgttacaata ccgattggaa
    14161 ctagatgata atgggaaaga aattcctgtt caaattagtg acgaaggctt agcaaaatat
    14221 gtctatactt cagggtatcg tcgatacagt agtaaaccag gtattatctt agctggtgaa
    14281 tcttctttgg cggtggccta tttatttatc ctatttacga tcgttacttg cattattgag
    14341 ctggtattta aaaaattcta tttagtgatt gccgcgtcaa cggactttga gacaggccaa
    14401 cggatgaaaa gtgcgataga agatatttct caatccttct tgttattggc gtttacgatt
    14461 ttagaattac gaatctatac attaatgctt tcaggtatcg gtgatttgca tgcggcagga
    14521 aagatcaatg gattcttgta tgttgttgct ttaattgtgc taactattgc gctattcaaa
    14581 ggctctcaat cggttacgaa gattttcggt gttgatacga gtctgaaaaa cggcagtaat
    14641 tctcttctta gcatgtttgc cttaggaaat attgcgaaaa atgtgggtag cggagctaaa
    14701 aaccttgcaa gtgctggtaa gagtggttta agccatttaa atgatattcg taaaaatgga
    14761 tttggccatc gaaaagatgt tacccctgat aacggggaag aacaatcaga cgataaagct
    14821 acaactgtac ctaatagtaa gaaaacagtt tccgaaatgt tcgggaaagc gaaaaatggt
    14881 gtttctaaag ccgcagaagg tcttggttac gtcaatgaac gtgggttaaa aggaacagct
    14941 gaagatctta cagaaaaagc caaagacacc ttagaagaga aattagacgg cacgagagct
    15001 aaagaattgg ctgaagctgt gaagaatcca tccgaagcac tagaaaaaca aaaagaaaaa
    15061 gcttcagaag tcaaagaagg actaaaagat gcgtatggtg aaggacaagt taatgcagca
    15121 gtaaaagcta atacaacaac cgaaaaagaa aatcaagaag gtatagtgat tccttcagtc
    15181 aaagatcacc aagaagcgtt gcactctaat gaaaattcga ttgaagagaa aaattccata
    15241 gaagaaagaa attcagaaga aggaaaaaat ggacagttac caaaaactga tccagaacaa
    15301 tatcgctata aacctaacag tgaccttgaa aatgagaagc gaatttcaga tttatcaaaa
    15361 gtaaatcaag aagacaaagc agaaaatgag ggacagttac caaaaactga tccagaacaa
    15421 tatcggtata gatctgttaa ctccgaaaat gaaaaacgca tttcagattt accaaaacga
    15481 aatcaagaag tacaaacgga tgaggattcc atcgttctgc caaatgataa tggaatcgaa
    15541 gttggaactg aaaaatcaac gaaaattggt ttacctaagg cacaaaattt accaatgaaa
    15601 gatagtcgtt tgaatcaatt caaggagaat gatcaaagct ttgttcacca atcaactgca
    15661 ggtactgttc aaatgaaacc aagtcgtttg cctgaaaatg gaaatctgac aactgtttct
    15721 tacaatttat ctgaaagtgg tgagacaggg aatgcgtccg ttcaaaatgt tgtgacaaga
    15781 gaagctgcac caaaacctat tcaaaacgtt catacaggtt acaagccagc aaatgtggca
    15841 tttactgcac caacaaaaat aaatgtgaca agagaaacta aaaacaggaa caatgcattg
    15901 aaagaacaac tattgaggga tatcaaacag gatatcaaac cataaaaggc ctacaagtta
    15961 ggccttttat ttatatgaaa aggagaaatc aattatggat aaatatataa acatgaaaaa
    16021 ccctccaaga tttagtggag aatggaagtt tttcgctttt ttctttttag atctattaac
    16081 gcttggtggc tgcttttttt gggcgaaaat ggtcaataat cttgtccact taccactctt
    16141 ttttgcactc ttgaattatg gcttatggat tgcgatagcg ttagtgattg tttggcgacc
    16201 ctataataac ccaggtcaac gacaaatttt tgcgattatc gagtctattt tttttgcgga
    16261 caagaaccat tatcatgcgt tagatcctaa ccgaaattat catggtacgt ataaggagga
    16321 tgaataaatg cgagtcaaag gacaaaattc agttgaaagg aaatccattt tgcagtttgg
    16381 aaaaccagaa gagcgctcta tcttggactt atttccgtat aaatcctgta cagaagaggg
    16441 agtcttaatt actagagaag atcattttca acgttattat cgtgtagttt ctacagacgt
    16501 ggaaggatta aatgaacaag agaaactaga acgaatgaat cagttgacca ccgtcatgcg
    16561 tacgtatgtg ccaacaatta agttagttag tttgaccaca gagacggatc taacggaaca
    16621 aatcgtgcaa aagcggcaat tattagataa aaatcgtatg gaacagtcct taggaagaaa
    16681 tctacaacga ttaaaaaaat atgaaaagaa attagtcgaa gaaatccagg aattgaaacg
    16741 agcagaacaa gaacgtcctg acttaagctt ctttttcgtc attgaaggaa agacaatcaa
    16801 ggatttacaa accagaaacc gtcagttaat gcgatctagt ggcattttag ggttaaaacc
    16861 actctacaaa aaagaattga tcaagattct gtatcgaatg aacaatatga atgatgagta
    16921 ggaatggaga agagagaatg aaatatccgt tgatatggtt agaaaaagat gttccgaagg
    16981 aagagaccac tagaaaattt cctcacactt taagcctggg taagttggga aaagggcaat
    17041 cttttaacca agtaaagaat caaacaagaa gaggtgactg tacatgttag aagcaattaa
    17101 aacagctttt tcaccacaaa atgatcaaaa agtacttgat ccattaaagc aactaggaaa
    17161 tcgggaaata gaaattttaa aatccaaagg gtatgattta gattttttaa aacaagtgat
    17221 gccacaaggt gggttaaatt ttgatgaaga atatgtgacg tatggtaatg gttattcacg
    17281 atgtgtgaca gtttatcact acacaaaaaa tcctacgctt ttttggttgg ccaatctcat
    17341 gaacaatcct catacagtgg ccacactcga tgttagcaca gatgaaaagc aaaaagtgat
    17401 tgatcaaatc aatcgtgcct tagatgaatt aaatgatcga tcagataatg aacgaaaagc
    17461 gactgaccga aataaaagcg cagaagaata cctggaacta gcggaatatg ctcgtaaatt
    17521 gacttctaat ggggaaattt ctaagcaagt caaagtgaga gtacatgttt acggaaacac
    17581 gattcaagaa gtagatgaac gagcgcagga actgatcgaa aatctaaaag ggatggatta
    17641 tcgtgcggtg acttatctgc ataaaaccaa acgagattgg caatcgatgt ttaccatgtt
    17701 cgatgaacaa gctgaatggt tcggaaatat caaagggcaa tcactaccat cgttaaacat
    17761 tggcggaggt ttcccgttcc accaccaaac gctaaaagat ccaggaggga cacaatatgg
    17821 ggaaaccatg acaggcggcc catttgtctg gaatgcgacg tataaaaata gtgtacggtt
    17881 gtctactaat accttgcttc taggaatgat gggagctggt aaatcgaccg ttcttaaaat
    17941 gatcgcggaa gcccatttag ctgcaggaga ctttatttgg ggctttgaaa agggaaaaga
    18001 ctttattcct ttcttaaagg aatataacgg tattatggtg cgtttagacg gatctgacgg
    18061 aatgatcaat cctttagaga tatttgccac acggacgtat gatgaagcat ccagtttata
    18121 tgacgatgga tcagtcaaag atttgaagat caatgaagca gcgtcctatc aaacacactt
    18181 agacaaagtg gtctatcagg tacagttagt gagtcctcag ctaaaaggaa cgatgaaagc
    18241 agaatttaag acctacttaa atcgttttta tgaagagtat ggaacagtac cacgcggctt
    18301 tacttcctca aattcgcgtt caaatacgga gacgcaagtc acaggtaaag atccagaagc
    18361 ttatccgacg ttcaaagaat ttttagactt tttaggacaa ctggaattac caggtgcttc
    18421 ccaagagaaa aagaatcgaa aagaagaaat ggaatccatc gtagaatcct tatgtgagac
    18481 gtatggcatg atttttgatg ggcatagtac gattcgacac cttgaccaac aacagttggt
    18541 cttttttgac attgactcta ttacaggctt agatcgaggg gtacaacaat gtcaaatgta
    18601 catggctatg acgttgattt ggaatcaggc attgattcaa ggaagaagac aacgac
//
LOCUS       DAYYHX010000041        18194 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000041 DAYYHX010000000
VERSION     DAYYHX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.041"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            341..508
                     /locus_tag="ACHA6Y_001028"
     CDS             341..508
                     /locus_tag="ACHA6Y_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987140.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            534..878
                     /locus_tag="ACHA6Y_001029"
     CDS             534..878
                     /locus_tag="ACHA6Y_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8987141.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            883..1164
                     /locus_tag="ACHA6Y_001030"
     CDS             883..1164
                     /locus_tag="ACHA6Y_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987142.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            1267..1581
                     /locus_tag="ACHA6Y_001031"
     CDS             1267..1581
                     /locus_tag="ACHA6Y_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8987143.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1559..3253
                     /locus_tag="ACHA6Y_001032"
     CDS             1559..3253
                     /locus_tag="ACHA6Y_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8987144.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3273..4451
                     /locus_tag="ACHA6Y_001033"
     CDS             3273..4451
                     /locus_tag="ACHA6Y_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8987145.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4414..5100
                     /locus_tag="ACHA6Y_001034"
     CDS             4414..5100
                     /locus_tag="ACHA6Y_001034"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8987146.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5100..6260
                     /locus_tag="ACHA6Y_001035"
     CDS             5100..6260
                     /locus_tag="ACHA6Y_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8987147.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNDFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            6270..7145
                     /locus_tag="ACHA6Y_001036"
     CDS             6270..7145
                     /locus_tag="ACHA6Y_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987148.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSNVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7142..7453
                     /locus_tag="ACHA6Y_001037"
     CDS             7142..7453
                     /locus_tag="ACHA6Y_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987149.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            7443..7796
                     /locus_tag="ACHA6Y_001038"
     CDS             7443..7796
                     /locus_tag="ACHA6Y_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8987150.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            7786..8187
                     /locus_tag="ACHA6Y_001039"
     CDS             7786..8187
                     /locus_tag="ACHA6Y_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987151.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8180..8584
                     /locus_tag="ACHA6Y_001040"
     CDS             8180..8584
                     /locus_tag="ACHA6Y_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987152.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            8596..9204
                     /locus_tag="ACHA6Y_001041"
     CDS             8596..9204
                     /locus_tag="ACHA6Y_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8987153.1"
                     /translation="MAKTKTVVTTFDNISIKRIAFNFKNAGNAIVTNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9224..9586
                     /locus_tag="ACHA6Y_001042"
     CDS             9224..9586
                     /locus_tag="ACHA6Y_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987154.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            9589..9771
                     /locus_tag="ACHA6Y_001043"
     CDS             9589..9771
                     /locus_tag="ACHA6Y_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987155.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            9789..13121
                     /locus_tag="ACHA6Y_001044"
     CDS             9789..13121
                     /locus_tag="ACHA6Y_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8987156.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFIQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGV
                     GEAGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMK
                     QTTQQIIDSITKIQNDNASAWR"
     gene            13172..13909
                     /locus_tag="ACHA6Y_001045"
     CDS             13172..13909
                     /locus_tag="ACHA6Y_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8987157.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            13919..16210
                     /locus_tag="ACHA6Y_001046"
     CDS             13919..16210
                     /locus_tag="ACHA6Y_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8987158.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYINDGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            16234..>18194
                     /locus_tag="ACHA6Y_001047"
     CDS             16234..>18194
                     /locus_tag="ACHA6Y_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987159.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDDQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAIN
                     KLRLVYRTSPGENILLEANNTTITIDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKSSGGNDSTILTIPEGYRPPISFEQLVGS"
BASE COUNT         6468 a         2704 c         3845 g         5177 t
ORIGIN      
        1 atgggcaaaa taaaaaatag tagcagccaa gaataatttt atagtgtcac tgtggcggaa
       61 agggtagacg cttaaaaata aggtcaatac gtcgagggat agccttaacg ttttatgatt
      121 tgaccatgca atgttcgatt cattgccagt gactttggca accgagggtt ggaaatgggc
      181 gctcaaagta cacgagcaag gcgaggtcga tagtaatcga tggaatcggt gtaggttgct
      241 tgatagagct ataactgcat ctcgtttatg aggtgtagtt tttacagatt agatcactct
      301 ttgagtggtc tttttatttt acacaaagga ggtaacaaga atgtatagac cacaatactt
      361 agaacagaag tatgaagtaa tcactgtgca aaacggtaac ggtgagatag tacgaaagta
      421 tagaagacca ataaagagcg atacatacaa acgaaaggaa agcaatgaag ttattccatt
      481 gtatggcaaa agaatagcta agcattaaat aagattgcga aaggagacgg aatatgaccg
      541 aggaattcta tagatggcta ttacagttga taagagaaga tcgtttagtt aagttctatc
      601 agtctcctaa atggcgcagg cttagagaga aagcgatgaa acgagatcac tatgaatgcc
      661 aagagtgtag aagactaggt aagtatcata gagtagaaaa cgttcatcat ataaaggaag
      721 tcaaggatag acctgactta gctttagatt tagataatct tatttgttta tgtgttgaac
      781 atcataatga agttcatggc agatatctta cagcattaga taaacaagag aagaagatag
      841 aaagctttgc taacttcgat gcaagtgaaa ggtggtaagt gcatgatcat caatgataat
      901 ggcagagagt atgatacaga aaagattgaa gagtattcat cttatactca gggattaatt
      961 aaacgtttga tatacgttcg ctatgtaggt attagggatc tgttatcaga taactgttgt
     1021 agtaaataca aagtgaatca agtaagagaa gcgttgaata aagataataa cgtcgaaaga
     1081 ataaaaaatg tttttggata tagtattgaa gagattaatt attacattga cttcgctgaa
     1141 gctttcattc cgatggtgag ataacccccc cttaaaataa atcgcaaatt ttttgggggt
     1201 gatgaaacgg agggggctgt caggaaaaga gattttttcg aactttatca tgaaaggagg
     1261 gctaaaatgt ttaaaaacga attgtctcaa aatcgctaca gagaaaaatt acgccgctct
     1321 ttaataagcc aattggaaag tcagaaaaca aatattgagc cattcttaga taatgttgat
     1381 cgttatatca gtttatggga aacggcgata tcactggaag aagatatatc cgagaacggc
     1441 attagactgg agaatggtaa aaagaatgaa tcagtagcgt tgcttgtttc tgtcaacaaa
     1501 caaatgggat tgatgttgga taaacttgcc attactcctg aattggtagg tgaagcaaat
     1561 gaatcaattc ctgagttata agcatattga aaattggttc aaagctatag aagaaggcac
     1621 tatcaaggta tgcaaagagc aattattgct aaaaaattat ctagaagaaa gagtctttac
     1681 tagagaagat atttacttcg ataagcagat ggtagaggat tcaatcaata taccagcaca
     1741 atactttcca ttcgaattaa ttccgtggga aaaatttcta caatgtttta tttatggtgt
     1801 tcgatggaaa aaagataaaa cactagtgtt caatagatat ctttcattaa tgggacgtgg
     1861 taatggtaaa actggttttg cttcttggaa caacttcttt ctactaaccg ctaaacacgg
     1921 tattaaaaat tatgatattg atatctatgc caataatgaa agccaagcaa agactagttt
     1981 tgatgatgta tttaaagtaa ttaaagatca tcctgattta gataaaaaag tatttaaagc
     2041 tacgaaggaa gttattcaaa atatcgctac aaacagcaaa cttcgttata acacggcaaa
     2101 tgctagaaca aaagatggga agcgaccagg tgcaaaccgc tttgatgaaa ttcacgaaaa
     2161 tgaagattat tcaatgataa atgtggctac ttctggtggt ggtaaaattc gagattatag
     2221 agaattttat gatacaacta atggtcatgt tcgtggtggt ccgcttgatg acattataga
     2281 agaatcaaaa atgattcttt ctggagaact tggaattgac aaggatggag cagaattttc
     2341 tagtttgttt ccatttattt gtcgcttgga taacgataat gaagttgatg atcccgacat
     2401 gtgggaaaaa gcttgtccaa ctattaatta caatgcagat ttaaaacgga aaatgtttca
     2461 agaatactct caaatgcaac gtaatgctgg tttaagactt acgttcatga ccaaacgaat
     2521 gaacagacct atggaagata cacgatttgc tgttgcttca tatgatgatg ttctgcatac
     2581 gaaagaaaaa gaatttcctg aaaaaatgga tgaagtgata ggaacagtcg attttgctga
     2641 tagacgagat tttgccagcg ttgggttgct aggaaaatac gataaagatg tttattttac
     2701 acaacatact tttatccacg aatcagccct tcgattacaa aacatcaaac gagaggttat
     2761 agatatttct atagatcaag gaaaatcaca gatcgttcat ggaaaaaata tagaagctga
     2821 ttatattgta ggttggtttc ttgaaatgag taataaatat tatattaaaa aaatcgctat
     2881 ggatatgtac cgtgcaaaaa tattgaagcc cgctttagaa gaagcaggtt ttactgtgga
     2941 aattgttcga agcggatctg ttacacatgg tatgttaaaa gatctggttg atgacctttt
     3001 tattaatcaa cgtttatttt ttggtgacga tgcgattatg cgttggtatt gcatgaatgt
     3061 atatgaagag catatttcta atggaaatat acgctatgaa aaaatagaac ctgaaactag
     3121 aaaaacggat ggcttttttt cattccttca tggtttgaat tttttagatg atatttatga
     3181 ttctgctcct gtaacagtca caaatagctc agtagaaaat acaggaactg gatttactcc
     3241 tctagtattc taacttgaaa ggaggtgaga aagtggggat ttttcaaaag gcggtaggat
     3301 acttcacaaa aaaagcaacg gttcctttag aagaatactt ttgtaaattg caagttgatt
     3361 ttgtgtatcg aaaatttgcg attgaaactt gtattgattt gattgcaaat gcgatgagta
     3421 aagcggaatt caagtcatat gaagatggaa aaaataaaaa gaatgatctt tactataggc
     3481 tgaatgtagc tcctaataag aaaaataatg caacagaatt tagaaaaaaa ctgatcagga
     3541 gattaatatt ctacaatgaa gtattgatcg tttctccgtc taataattct agcgaaatat
     3601 ttattgcgga tagttgggat gtcacagaat atgcattgaa agatgatgtg ttttctcaag
     3661 tgcaaattaa caacatagtc cttgatagag aatttctaga aagtgatgtt atctatataa
     3721 aatacgcaga tcaacaaatt aggcaactag tcgatgcgta ttatcaagcg tatgggaaac
     3781 tcatttctag tgccatgaat gtttacaagc gctctaacgc tcgtagatac gtactgaaag
     3841 ggaatttatt ccgaccgcaa gacaatacaa cacaagatca aatcaataaa atgatgacat
     3901 cacaatttaa ggcttttatg gaagctgata atgcaggtgc ggtatttcaa ttacaaaatg
     3961 agtacacatt agaagatttc agcggaaact tccaaagcaa ttcaagagat ataaaaaact
     4021 taatagacga catctttgag atgacagcag cagcgtttca cgttccgaaa aacctactaa
     4081 agggagacat gagtgggtta tcggatcaag tggacgcttt tttaatgttc gaaatcatac
     4141 cgattgctga acttattcag gatgcgttta acgctagtct ctatgaagta gaagaatact
     4201 tgtcagggaa ttttgtacgt gtggatacaa ctatgatcaa gattactagc ttcaaagatt
     4261 tggttgacgc tattgatgta ggcattagaa atggagtatt tacaatcaac gaaggaagag
     4321 aacgcgttgg aaatgatcgc tctgataagg cgatggcaga tgaaatattt ataactaaaa
     4381 acaatcaaca agtatcgaaa ggaggtgagg cgaatgacga caatgaaaac atttctagca
     4441 gtaaagaatg aaggcgcagt accgcaaatt tttattcagg gatttattgg ttctagttgg
     4501 ttctttgaag ggaatactga caagggaatc aaaaatattt tggatagtct aggtgatcaa
     4561 gaagaaattg aagtagtaat taattcaaac ggtggagacg tatttcaagg gattgctatt
     4621 gggaacttac ttaagtcaaa taaagcaaaa gttaacgttg tgattaacgg cttagccgct
     4681 agtgctgctt caattatcgc aatggctggc gatactataa aaatctacaa caatgcacaa
     4741 ttgatgattc accgcgcttc cacatacgga gaaggaaatg tcgatgactt ccgtacgatt
     4801 gctgaccaac tggaatcaat tgataaatcg gtaaaggctt catataaaac acgattcaat
     4861 ggcacagatg aagcattgca agaacttctt gaaaaagaat cgtttatgga tgcagaaaca
     4921 gctttgagtt atggattggt cgatgaaatt atcgatgcag aaaatagctc aggtactgaa
     4981 gctaaaaaag aacaaagcgt tgaagaaatt ttgaatgacg ttaaagaaaa aagagcagaa
     5041 aaaattgctg catttacagc agcattaaat aaaacatttg gacaaggaga tgtaaaataa
     5101 tgacagttaa aaatttaaaa ggtgtaacag ctgcaagcga ccaattgatg aaagctttta
     5161 aagatggtaa cgaagaatct tttagcgcag ctatggtaag cttatctaag gaaattcagg
     5221 ataaaatttt agaagaagca acagcaaaaa atcaagatca attagtatta atgaaccgtg
     5281 gtcagcgtgt attaactaca caagaaacaa aattctataa cgaagtagtg aaaaacgaag
     5341 gttttgcagg ggtcgaagaa ttagtgccag ctactgtatt tgaacgtgta tttgaagatt
     5401 tagaacaatc tcatccacta ttgcaaaaaa ttacttttgt taacacaact ggtgtaacag
     5461 aatggattgt gtcacgtgga gtcaatccag catggtgggg taaactttgc gaagctgtta
     5521 aaaaagtttt agataatgac tttgatgtaa ttaacatgaa gcagttcaag ctatcaggtt
     5581 atattcctgt atgtaaggca atgcttgatt taggtccagt atggttagat cgttatgtcc
     5641 gtactgtttt agtagaatca ttgagaattg cattagaaca agcaattgtt gatggtactg
     5701 gtaaagatat gccagtcgga atgatgcgtg acatgagcaa acaaactagc ggagaatatg
     5761 ctgaaaaaaa agcagaacct attacagctt tagatgctgt aactatgggc ggtttgatgg
     5821 cgcgactatc aaaattcaat atcgaaggtg tgaatgatcc gatttatcgt aatgtgaatc
     5881 cttctgatgt ggtcctaatt gtgaatccaa ctgattactg gtctaaagta ttcccagcta
     5941 agactgtact aactgctaat ggagaatacg tacaagtatt gccagtacca gtttcagatt
     6001 tgcagtcaac ggctgtgcca gaaggaaaag cagttattgg ggtagcctca gattacttca
     6061 tgggtgtagg atctacacta aaaattgaag cttcagatga ataccatttt gttgaagacg
     6121 aacgcattta tctagctaaa caatatgcaa acggtcaacc taaacgtaac gatagtttca
     6181 ttgtattaga tattagcgct ttgggaacta ctactacaac tacaaaacca acaaccacaa
     6241 caactacaac acaagcgtag gtgatcagaa tgaagtatat tctttgtcag ccggcaatca
     6301 atcggtttaa atgggagctt gaagtttgtt taactaatct gaagaaacta ggaatcaaag
     6361 atatcgtatt gcttttcagc agacacgatg atcagattcc tatttttttt gagaaggaat
     6421 atggtgttga agttcatgtg tacgatgatc tgcgggacga caaagagtat attccttcga
     6481 ttaaaccata tttatggtgg aaatatttag aagaagatca ttcgcgtgag gacgaccgat
     6541 atttctatat cgattcgaat gtcattttca ataaaagaat taatttgcgc aaattgcctt
     6601 ctaaagatga tgtttggtat tgtagcgact gctgtagtta tctaagtctt gattatatta
     6661 gaagctgtga aaacggagaa aatattctaa aagatatggc aaacattgta aatgttacag
     6721 tagaatcttt ggaaactata aacactaatt caggaggcgc acagtgggtt attaaccgtc
     6781 ctaaagcgaa ttattggaaa aaggtttatc tggattctaa tcggctatat cgctacctta
     6841 gagggcaaaa aacaaatata caaatctgga cagccgagat gtgggcacag ctttggaaca
     6901 tgatgtattt caatattggt cctaaagttc acgaggaatt agacttttgt tttgctactg
     6961 atccaataga aaaagttaaa gaagtaaaaa tcttacacaa tgctggagta acaacaaacg
     7021 atgaagattt atttttcaaa gggagatacg tgacttctac gccttttgat gaagatttat
     7081 catttgtaaa caagaaaaaa tgctcttacg catatgttaa agcaattaag gcggtggtta
     7141 gatgacgcct gaacaagtga ctgaagaatt gctaatagct gtgaaggata atatttacgt
     7201 tacctggaac gaagaagatg agtcaattaa aaagatgata gctaaaaatg ctgtttatct
     7261 tcaaagtaaa gtgagtacaa cactttcttt ttctcctgaa agcttagaat acggattgct
     7321 aatcgaaaga tgtagatacg actggaatcg tgctttagat gagtttgaac aaaatttcgc
     7381 tagtgagtta ttaggtttca ttcaacatta tgcgctacaa gaatatattg caggtgatgt
     7441 gaatggcgaa taatcgtaga ctcgaagaaa cattcaacga tggttggtta aagattttga
     7501 cgcaaactac caaaagaaat gaactaggaa aaaagattgg tgtagaagat acagaaatca
     7561 cttctttaaa atttagaaat ctttccatga gagatagtga tataacagct atggatgcga
     7621 tgggatcgaa attaactaag aaagtaaaga ctccatttca tccaatcgcc aagaaattta
     7681 ataaagatca atattttatc gtaatcgata gtatgcgtta caacgttatc tatgccgatt
     7741 acgataattt ttatatctat ttttatcttg aaagtgtggg tgaatatggt gattgataat
     7801 tctaaagaaa aagaacgttt aaataagcaa atttctgcta tcaaaacttc cttagaagag
     7861 cattttggcc tcaaactctt tcaagactcc gtaggcgagg atgagctacc tgatgatttt
     7921 aattacttca ttctcgaaac aggagaaata gaaatgatca ctgagccaaa atatagcgtg
     7981 ggtcaaaatc tatatctaac tttctattca gaaaatagag aagatttaac aggagactca
     8041 ctagatatta tttcattgat tcaaaatcgt tcgattcgtt ttcagagaat ggatcccaat
     8101 catttaaaac tagagaacca agatcgctat atcgatcaat tggtatttac gtttagacga
     8161 ttattgaaga gtgattgtca tggctaaaaa tagttgggag ctaaaaataa atggacatga
     8221 tgaacttctt gtgcggatgg aacgctattc aagcgagagc gaacgactga ttaacgaagc
     8281 attgaaatca aaaggttcgg ctattgcagt ggataggatt acagaaaaaa ttcctgtttc
     8341 tgaagcagat ttaagaagag ggcaccaaca cgcaaaaaat agtcgtccac ttaagactca
     8401 atacattaat ttgggtttca tcattagacc tacaagaaaa tttgagtatt taaaatatcc
     8461 tgatttgggg ataggtactt ctaaaagaaa tcagccggac gaatttatga gaagaggatt
     8521 aggtcttgca cttgatccaa ttacagaact tctgattcgt caattcgata aattaaataa
     8581 atagggggaa caacaatggc taaaacaaaa actgtagtaa caacgttcga taacataagt
     8641 atcaaacgaa ttgcttttaa ttttaagaac gcaggaaatg caatcgtaac gaattgtaat
     8701 ggacaattag atggcgaaac agaaatgcaa acggtggtta aaaaatgtgg agcgacagaa
     8761 gtaaaatcaa aatctaaacc aatcaatatg acggtaacaa ttactgcaca tgtaccgatg
     8821 gaagtttatc gacgtttcaa tgggttgaaa caagatggac gtattaaacc aggcatttac
     8881 tcttacggtc ctgattccgt aggcgaagat ttctcacttg ctgcagagat cgtggatgac
     8941 ttcgaagaaa atagaaagtt agttggtatg ttagcatgca cttcgaatac aggattaaca
     9001 ttctctattg aaaatggtgc ggatgaagta gctgcgttag aactagaaac aaaagttatg
     9061 caagatgaat ttggtaaatt ttatcatgaa gcaattgttg cagaacttga agaagactta
     9121 acagatcaat ggatgacgaa tctatctgct gatgtgatta aaaaaacttc agttgtgaca
     9181 actacggcca ctcaatcaca gtaaaaaaaa cggaggtagc gaaatgaacg aagattactc
     9241 aaaaattgaa ctaaacgatg gaacaatttt gaatttagaa cctaaactga atatcaagaa
     9301 attattgatg atcaatagag attttaacac agacgagttt gcaaaaatgt cgatgggaaa
     9361 aggctctatg gatattacag ttattcaagg tgcaaaagcc gtatatgtag cttatcgtca
     9421 agcgaacatg gtcgattaca tttcattcga tgaatttata gataaatggg attttgatat
     9481 ggaggttgca gtagctgtat acagtactat gatgttcaaa caagcacgtg atgcctatca
     9541 aaaagaattc gaaaaagcaa ataaggaaaa aaagcttcaa aagtaaaaat gccaaagctc
     9601 ttagttgaaa cgtgggtcga tgtctattcg atgttgaccg acgttttttc tatgccttca
     9661 gatttggttt taagcgatat ctgtttagat gacattttgc aaatggctta caacaagagc
     9721 gcttatgaag gatggaagaa ctatgcaata aatcaatccc agaaaaacta aaggaaagga
     9781 ggtaaaaaat ggctaaaaag agaacggaag cagaagtaac tttcatagct aacgatgacg
     9841 gattgaaatc tacgttaaaa gaaatcagtg ctgaattaac taaaaataga gcagaattaa
     9901 aactagaaca agctcaatta caacagactg gttctgaatc agacaagtta ggaagtaaat
     9961 tatcttcttt agaaaagcag tatgaattac aaagtcaaaa agttgaagta actagtcaac
    10021 gtttagccaa tgccaaaaaa tattatggag aaaattccac cgaagttcag aaacttgaaa
    10081 gagaactgat taatcaacaa acagctcagc aaaggttatc taatgatata gataaaacga
    10141 gtaaggcatt ggctcaatct aaaggtgaat ttaaaacgta tgagtcaact atgaaagact
    10201 tagataatga gcaacaacat ctaaagtcaa gcgcatcatt agtagaatcc gaatataaaa
    10261 aatggcaagc aactgctggc cagtctgcta cagaatctga aaaattagca aaagcacaag
    10321 aatatgtcgg caaacagagt gatattgctg aacaaaaaat cgaagtgcta aaaaaacaat
    10381 tagatgccac acaaaaagaa tttggagaaa catctaccga agctttagaa atgaaaacta
    10441 agcttaatga tgcggaaaga gaattcgaag aattaagtaa tgctgctaaa aacgttgata
    10501 cctccacagt agatgatatc ggcaaaaaac ttgatatggg taatttaatg gaagctactg
    10561 atcacttgtc agtgatcgga gataagctta ttgatgtagg tagtaagtct attgaagcag
    10621 ctggaaaagc acaagctatg caggcccaat ttaaacaagt ctttggctct ttagaagggg
    10681 aagcacagga cgccgttgag ggaatggctg aagaatttgg aatgttacca aatacgatca
    10741 agcctgtttt tatacaatat acgtcaatgt ttaaaggact tggatatgat accaaagaag
    10801 ctatggagtt agctggtgat agtactcagt tagcagcaga tgcagcagct ttttacgata
    10861 agtctatgga tgatgctagt gaatctctta attcatttat aaaagggaac tacgaaggtg
    10921 gggagcaaat aggtttattt gctaatgata ctcaaatggc agcttatgct gttaagcata
    10981 atttgatacc agcgactgaa ggagcaaaaa aagccagtga agagtcattg ttagctgttg
    11041 aaaaagcaca atctaagtat gctgatgcta ttaagaaaca tggtgaagga tctttagaag
    11101 caagagaggc tgctttaaaa cttaaagatg cgcaagataa aataaatgaa gaattaggcc
    11161 cacaaacgca aaaatggtct gatttagatg aggctaccaa gcaagcagtt cgagttcaat
    11221 atgctgaaga tatgcaaaaa ttagccggtg ctacaggtca ggctagtagg gagtctgatg
    11281 gtttagaaaa ccaaatgaca agagcaaagc aagcgttaga agatttttat gcctcattgg
    11341 gtgaagatat actgcctgtg tttatcaaag ggttgcaagc aggagcgaaa gctttgcaag
    11401 gattagccga atggtggagt aaacttgatg ggccaatgaa aaatttcatt ttagctcttg
    11461 gaggaattct agcattatta agcacattag ctcctgttat aaccgcagtt gttacgatag
    11521 ttggcacatt tggttctaca gttttgctgc caataatagg aattatagcg ggcgttgcag
    11581 ctgtaatagc gattgtaatt acagcgtttc aaaactgggg cgcaatcact gactggttta
    11641 gtgatttatg gaaaaaattt accgattggt taggtgatac ctgggaaagt ataaaagaaa
    11701 gtgcctcatc agtttgggat ggagtcaaag aaacctggtc tggatttgta gattgggttc
    11761 aagaaatttg gcaaggagtt tctgattggt ttggagagtt atggagcgga ttagttgaag
    11821 gagcttccaa catctggcaa ggagtccaag agacttggca agcattcgtt gattgggttt
    11881 caaatatttg gaacggagtc aaagaagtat ggtcgattat ttgggcagac attgtaggaa
    11941 ttgttcaaat accatggaca ttaataacgt cattgattca agctggtatt aatattatcg
    12001 tgggtatctt tgatgtagct ggacagttat taggcgcagc ttggcaagct gtttggacac
    12061 ctatttctga tttccttaaa aatacttggg atactatgac acaatggata agcatcgctt
    12121 ggaatggaat tgtaactaca ttccatacta tatttgatcc agtagtggca tggtggaatg
    12181 gtatatggac atctattagt actacggctt caaatatttg gaattcaatt agtgcaacag
    12241 cttctagtat ttggaacagt atcaagaata caatcactag cttggtacaa gcagctgcta
    12301 cagtaattca aaatatttgg tcaactgtat ctagttggtt aggtggaatt tggaattcaa
    12361 tcagctctac agcatcaaat atctggaata gtgtgactag tagtataagc aatgctataa
    12421 acgcagctaa aagtgccatt caaagtgttt ggaatagtat atcttcgtgg atcagcggaa
    12481 tttggaacgg tatcaaaaat actgctttga atctttggaa tggaattaca agcactatta
    12541 gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg ttggtccaat ctaacaggta
    12601 ttgtttccga catattcaat aatgttaaaa gtacaattgc taacatatgg gaaggcatca
    12661 aaaagactgt tagcgctcca attgattgga ttagagacaa aatcagtggc atttttgata
    12721 atttgaatat ttctatacca catattccgt taccggaatt tatcatggaa ggcagcttta
    12781 acccgctaaa aggtcaaatc ccccatttgc gtgtaaaatg gcatgctaaa ggaggtatct
    12841 ttactaaacc aactttacta ggtggaatga acggtgtcgg tgaagcagga cctgaagcag
    12901 ttttaccttt gaaaagatct gttttgcaag aaattggtga tcgtatcttg agtagcacat
    12961 cagtttcatc tagggcacaa acgattcaac ctgtgaataa ctacgaattc aatttcacaa
    13021 ttgatggtaa cgcagatgag gttactatga agcaaacaac tcaacaaatc attgatagca
    13081 ttacaaaaat tcaaaatgat aatgcttcgg catggcgtta aacaggagag tatttctcct
    13141 gtttttttag tattaaaaag gatgtgaaaa aatgactgat tgtatacatt ctataatcga
    13201 tggatttcct gattatttgc ataaattggc tttagctgaa agaccaacca taccttctcc
    13261 aaaaagacag agagttgaaa cttctgtttt aggtaggtta ggtggcttag tacaagatta
    13321 ctcgtttgaa gatatgtcgt ttacattgca ctataactat ttagaggatg tggaagacca
    13381 tcaagcgttc aagcaatcgt tttatatcat gcgtcattgg ttaaactatg caaagaaatt
    13441 agaattctct gacgatccca acgtctatta cgttatccag actatcgata ttggggatgc
    13501 agaaaacgat attgttgaat ggggagagtt cgatgtaaat attactgcga aaccattcgc
    13561 aagagttcaa gaagatgtac ctataaccgt agataaacca cagtcattta acttgctgaa
    13621 taatagttta gaagaaagtt ttccaaagat tatcatcact ccttcagcta cttcatgcca
    13681 gttcatctta aatgattatg tgtttagttt cgaaggctta gtagcaggaa ctgatgtagt
    13741 cattgatagt gatttgatgc tttgctacga agagcaatcg gacggagata ttttagatcg
    13801 gtccaacaaa atgaagacca tgcaatatcc aacgttgcaa gtggatatta atcattttaa
    13861 ttgtactggt ttgagcaaaa tacaaattta tcgtaacggg ttaaggtagg tgaaatagat
    13921 gatcgataat ttaataacta tttacgataa aaatgacgcg aataatttag ctgaacattt
    13981 atatgatacg caaggtttag gcgctttgtc agactggtta acagctactg ttagcaataa
    14041 actaaacgga gccgagatat ttcagggtac ttattcaata aacggaacta atgcagattt
    14101 gattgtagaa ggacgtatta ttcagtgtta tgtagatgaa aatcgagcaa aacagcgtct
    14161 acggatttat tatgcaaaaa cttctgtaat aggcaatacg atagaagtaa aagctgaacc
    14221 tattttcaat gatataagaa aatcggtgtt gaataaatat gacagcggaa cagaaaagat
    14281 cactgctact caggcatggc aaaacgcaaa agttttagcg aaaccagcta tcccttcgca
    14341 gttttctttc tcgtcattag tagatacgct tgctaatgtg aagatagaaa aggcgaattt
    14401 tttagaattc tttggtggaa aagagggatc tattctagat cgatttcatg gtgagtttct
    14461 aaaagataat aacacattac gtcatgaaaa aagtctaggt acggatcata aaatcaaagc
    14521 gatttatact aaaaacttaa ctggtcttga cttagagatt gatgctcaaa gtgttttagt
    14581 tggagtttat ccattcatta gcagctcttc agaaggagaa gacgagatca ctctaccaga
    14641 agaagttatt ttcacggatt acgtggatga ttatcctgct ggatatgttt cttttgttga
    14701 ttttaaagac aaagcgactg atgtagccac attaagggaa gctgctaaag actggttgaa
    14761 aacaaacata gataaacaaa aaccacaagt gagtggttcg attgaattag taccattgag
    14821 gcatcaaaga ggctatgaaa aatttgttga tctagaaaaa gtttcgatgg gtgacggagt
    14881 agatgtgtat catccacagt taaaagtgaa tatgtcagcg agaattgtgg aatatacgtt
    14941 taatgttcta accaattcat acgataaatt agttgtagga aacgtcaaaa caaacttctt
    15001 agaaaacaca gagaataatg tcagcaattt gattaatgat gccattgatc aattgaaaaa
    15061 tggtggcgaa atcagtgatt taatcaatga tattgtagat catcaaactg atatgattac
    15121 tggtcaaaat ggtggttacg ttttattaga tcctaaagaa gcccctagtc gtattttgat
    15181 tatggacaca ccagataaga atactgcacg gaacgttttg caaatcaaca acgctggtat
    15241 tggtttctct aaaactggca ttaatggaac gtatgacacc gcatggacgt tagatggcgg
    15301 attcaatgcc tcgtttatta cagctggtga gatagtaggg attactatta gaggtactac
    15361 attaattagt gatggcactg attatagaac aagtatagct aatggcaaaa tgacttggta
    15421 ctcaaaaaaa gttaacaaag atattatgga gctagaagca cgtgattatg taagtgctga
    15481 tgccggcatt gtatcataca ccatgaaaac tggtggtggt ttcatgatta gaaatccaca
    15541 gggtaacttg gtttttagta cgtgggataa tggtaataac agaccgtttt tatcttttgg
    15601 tgcgccaaat ttcagataca gtaatgctag ttatataaat gatggtgacg gtaattcttt
    15661 aggtattgat ggtagtgcag gtaattcatg ggaatttaaa gtagctggta ggactatgaa
    15721 atttactagt gacggtatgc taacgttgcc aggttgtttt tttggttcat gggaagatgg
    15781 gaaacttgct agatttgaac aatcaacggt acaagtatat aaagatttta ctgttagagg
    15841 tactaaaaac tcaaccgtac caacagaaca ttatggacaa cgactattga acgcttatga
    15901 aactccagaa tattatttcg ctgattatgg ggaagccgtt acaggtgatg atggtaaagt
    15961 tcgtgttgat attgacccca tgtttgctga gacagtaaat ctaagtcggt atatgacaca
    16021 tgtgacacct acagaactag ttttgtgtgc tgttactcat gaagatgttg accatttcat
    16081 cattgaaact agtaagccaa acgtattggt tagatggaat ttagtggcac atcgactagg
    16141 gtatgaagat gttagattaa aagaggatac agcatatgat agcacagtgc ttgaccaaaa
    16201 acgtttttaa aacgaagaca aggaggtata taaatggcta gcagtttata taatttggct
    16261 ttagatttca gcaaagaatt aaactacacc aaagctatta tggctcgtca aggtgataaa
    16321 gggatcacgg tgactgttaa accgttttta aatggcttgc agatggatac gagcggcgga
    16381 acatttactt taaaaggaac tacgccatct aaccgttacg tagatagtgt cgctactagt
    16441 gtaactagtg aagaagtcac attttctctt gatggcacat ttatgagtga agcagggtat
    16501 tataaacact gctatgtaga atatagaaaa gacgatcaaa ttttaacgac gcaagatatc
    16561 atttttttct cactaggagt gtctgacatt tcgcaaggcc aagccgatga atatgtttcg
    16621 caattagaag aattgattcg aaagtataac gaaacttttg atgcttttct ggctgaaatt
    16681 aaaggtagaa tggatagctt aaataaacag attactgatt taactggtca agctaaaaca
    16741 ctacaagaca agttagatgc tctgaaagaa gaaatttcta aattaggtaa cttacatgtg
    16801 atgtactcca acagcatcga cttcgggggc tatgattata gtgggaatcc gaatttaatg
    16861 tccaaactaa aatcgagcga ttttaacgtt ggttaccacg ggtcactaac taatgttaac
    16921 ggaatgttac attttatgtc tgatggtaca ggggacattg atatgtttac gcgtattaat
    16981 acacctcagc ttgctagtgg caaaacatat actctgagtg caaaagttcg atttgatgaa
    17041 ggaactacag gagctattaa taaattacgt ttggtatatc gtacatcacc aggagaaaat
    17101 atattattgg aagcaaataa tacaactatt acaatagatg atgtagggaa agaaataaca
    17161 atcaaaggca cagctaacgt taattatcaa atcacaaatt tagatcgttt ttatattagt
    17221 gttagttttt ctaacaggga taaaataaat ggtggattta aattgtacga catcaaaatc
    17281 gaagaaggct caacagccac tccgtatcag ccaaacttac tagatgaccc ttactgggta
    17341 ggtaaaacat cgttgggtaa gaatattgca aataaaggta cgacgttccc aattaaatct
    17401 agcgcgtacg aaatatataa aggtaacacg gaagaagagc ttataatagg tcaaacgtat
    17461 actatcacgc ttaaaggaac aaaacccgca agtcaaacct ttgtagcata taattattgg
    17521 aacgttaatt ttggagatct aaaaccagtt gagggattga cagacgtatg gtctctaaca
    17581 ttcacaccaa cgaaacttga accgggtttg cctaaagacc ttcgcatttt tcagtcgcct
    17641 aaagaaacgg ccggcgcatg ccgaattgac tggctcaaga tcgaaaaagg cgacacacga
    17701 accccgaata ttgagcaata taaataccga ggaatcggca tgcgagactc aaacaatcca
    17761 aaagattatg tatgggatct agaacctagc tatgttgaga ataacctagc tacagatatc
    17821 aaggttgctg aaatcacagg taaagcaaac aattataccg atgggaaagt atcggagatt
    17881 aattcgcagt tgactgcttc aattaatgaa gtagacacca cagctaagga tgctcaaaca
    17941 aaagcgaatg ctaatgcgac tgctatagat gaattagaca ataagatcga tgaacgcatt
    18001 aatgatacag ctactaccac attaacagtt acaaacggga ataccggatc agcaaagctt
    18061 tatcgtgaag gaaaaacagt ttctatatat tttgtggctt taaacggaaa aagcagtggt
    18121 ggaaatgatt caacgatact aacaattcca gaaggctatc ggccaccaat tagttttgag
    18181 caactggttg gctc
//
LOCUS       DAYYHX010000042        17540 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000042 DAYYHX010000000
VERSION     DAYYHX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.042"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..996
                     /locus_tag="ACHA6Y_001048"
     CDS             139..996
                     /locus_tag="ACHA6Y_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM8987160.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1111..2031
                     /gene="pstC"
                     /locus_tag="ACHA6Y_001049"
     CDS             1111..2031
                     /gene="pstC"
                     /locus_tag="ACHA6Y_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM8987161.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2031..2915
                     /gene="pstA"
                     /locus_tag="ACHA6Y_001050"
     CDS             2031..2915
                     /gene="pstA"
                     /locus_tag="ACHA6Y_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM8987162.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2930..3736
                     /gene="pstB"
                     /locus_tag="ACHA6Y_001051"
     CDS             2930..3736
                     /gene="pstB"
                     /locus_tag="ACHA6Y_001051"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8987163.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3752..4510
                     /gene="pstB"
                     /locus_tag="ACHA6Y_001052"
     CDS             3752..4510
                     /gene="pstB"
                     /locus_tag="ACHA6Y_001052"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8987164.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4523..5200
                     /gene="phoU"
                     /locus_tag="ACHA6Y_001053"
     CDS             4523..5200
                     /gene="phoU"
                     /locus_tag="ACHA6Y_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM8987165.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5777..6469
                     /locus_tag="ACHA6Y_001054"
     CDS             5777..6469
                     /locus_tag="ACHA6Y_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8987166.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6466..7233
                     /locus_tag="ACHA6Y_001055"
     CDS             6466..7233
                     /locus_tag="ACHA6Y_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8987167.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7333..8901
                     /gene="liaX"
                     /locus_tag="ACHA6Y_001056"
     CDS             7333..8901
                     /gene="liaX"
                     /locus_tag="ACHA6Y_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM8987168.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9198..9518
                     /locus_tag="ACHA6Y_001057"
     CDS             9198..9518
                     /locus_tag="ACHA6Y_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM8987169.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9518..9868
                     /locus_tag="ACHA6Y_001058"
     CDS             9518..9868
                     /locus_tag="ACHA6Y_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM8987170.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10035..10973
                     /gene="hprK"
                     /locus_tag="ACHA6Y_001059"
     CDS             10035..10973
                     /gene="hprK"
                     /locus_tag="ACHA6Y_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM8987171.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10988..11818
                     /gene="lgt"
                     /locus_tag="ACHA6Y_001060"
     CDS             10988..11818
                     /gene="lgt"
                     /locus_tag="ACHA6Y_001060"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8987172.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11862..12887
                     /locus_tag="ACHA6Y_001061"
     CDS             11862..12887
                     /locus_tag="ACHA6Y_001061"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8987173.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12902..13840
                     /gene="galU"
                     /locus_tag="ACHA6Y_001062"
     CDS             12902..13840
                     /gene="galU"
                     /locus_tag="ACHA6Y_001062"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8987174.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13965..14405
                     /locus_tag="ACHA6Y_001063"
     CDS             13965..14405
                     /locus_tag="ACHA6Y_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM8987175.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14408..15010
                     /locus_tag="ACHA6Y_001064"
     CDS             14408..15010
                     /locus_tag="ACHA6Y_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8987176.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15078..16184)
                     /locus_tag="ACHA6Y_001065"
     CDS             complement(15078..16184)
                     /locus_tag="ACHA6Y_001065"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM8987177.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16394..17395
                     /gene="ccpA"
                     /locus_tag="ACHA6Y_001066"
     CDS             16394..17395
                     /gene="ccpA"
                     /locus_tag="ACHA6Y_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM8987178.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
BASE COUNT         5727 a         3044 c         3781 g         4988 t
ORIGIN      
        1 atagcataga aacttttaat gagatagttt tttttacaat tatgagaatt tattttgtta
       61 tttaatgatt ttgattcgtt ttcatcaagt aatgaaaatg ataaaataaa catactttag
      121 gattaaagga gatttctaat gaaaaaactt aaattattct taccaataat aggattagcc
      181 attcttttat caggctgttc aaagtggatt gatcgaggag aatccattac ggctgttggc
      241 tcttctgctt tacagccttt agtagaaaca gttgctgaaa cttaccagac agaaaatcct
      301 ggaaaattca tcaatgtaca agggggcgga agcggaactg gtttgtctca agtacagtca
      361 ggagcagtcg acatcggaaa ctctgatttg tttgcggaag aaaaatcggg gatcgatgcc
      421 tcagcattag tcgatcatcg agtagcagtt gttgggatca ctcctattgt gaacaaggat
      481 attggcgtaa aagacatcag tatggaaaac ctgaaaaaac tatttacagg cgagatcaaa
      541 aactggagtg aagtcggtgg aaaagatcag ccagtagtta tattgaaccg cgcttcagga
      601 agcggaaccc gtagcacgtt tgaaaaatgg gtattggacg gagaaacagc tgttcaggca
      661 caagaacaag attcaagcgg aatggtgcgc cagatcgttg ccgacacacc cggtgcaatc
      721 agctatgttg ctttttctta tgtttccgat gatgtagata cgttgaatgt taacggtgta
      781 gaaccgacag atgaaaatgt gacaacgaac aaatggacga tttgggcata tgagcatatg
      841 tacacaagag atcatccaaa aaaactgaca aaagaatttt tagactatat cttatctgat
      901 gaagtacaag ataacatcgt tggagaacta ggttatatcc ctgtttctca tatgaaagtc
      961 gaacgtgatt ggcaaggaaa tatcgttaaa gagtaatctt tacacaaaat ttacaaactt
     1021 agctaaactt ttacatgtta ttaaaaccaa attgatgttc aatttgtaca ctgagtgagt
     1081 atgttaaacc tgattaaggg ggagtcatct gtggaagatg tgaaaaaagt attattaaca
     1141 aaatcaaaac gttcaaaaat ggaacagcgt ggaaaattca tcagtttttt atgtatcgcg
     1201 ctgatcgtgt tggttgtatt atctattttt tattttgtag ctagtaaagg attggccacc
     1261 ttttttactg ataagatcaa tgtatttgat tttctgtttg gtacagattg gaatccaagc
     1321 caaatcggag caaatgggaa accaatggta ggggcattgc ctatgatcac aggttcgttt
     1381 attgtgacct ttttatctgc tatcgttgcc acgccatttg ccattggtgc agcagtattc
     1441 atgacggaga tctctcctaa aaacggaacg aagatcttac agccggtcat cgagctgctt
     1501 gtaggtattc cttctgtcgt ttatggtttt atcggtttat cagttatcgt gccttttgtc
     1561 agaagtattt ttggcgggac agggtttggt atcttagctg gtacatttgt gttgttcgtt
     1621 atgatcttgc cgactgtcac aacgatgact gttgatgcat taaaggcagt gccacggcac
     1681 tatcgggaag cttcattagc gcttggtgcc acaagatggc aaacaatcta caaagtcgtg
     1741 ctacgagcag ccgtgcccgg gattttaaca gcagtcgttt ttggtatggc tcgtgcgttt
     1801 ggtgaagcct tagctatcca aatggtcatc ggtaatgcag cattgatgcc aacaggactt
     1861 actactcctg cttctacatt gacgtccatc ttaacaatgg gaatcggaaa tacaatcatg
     1921 ggaacagttg aaaataatgt gttatggtca ttagcgctga tcttgctatt gatgtcatta
     1981 ttctttaata tcgtgatccg tatgattggg aagaaaggag agctgaaata atgaacgcaa
     2041 aacgctcaga taaaattgct acaggtgttt tatatatagt ttcggggatc atcgtattga
     2101 ttttagcagc tttactactt tatatcttag ttcggggaat accgcatatt tcatggtcct
     2161 tcttgaccga accttccaaa gcctaccaag aaggcggtgg aatcgggatc cagctattta
     2221 actcgattta tttgcttttg atcacgatga tcatcagttt tcctatttct ttaggtgcgg
     2281 gtatctatct ttcagaatat gctggaaaaa actggctaac agatgtgatt cgcacgtcga
     2341 ttgaaatctt gagctcgttg ccttctgtcg ttgtcggttt attcggtttc ttggttttcg
     2401 ttattcaatt ccagtatggt ttctcgatta tttctggtgc attggctttg actgtattca
     2461 acttgcctct tttgactaga acagttgagg agtctttaca agcggtccat tacacacaac
     2521 gagaagcagg attggcctta ggtttgtcac gctgggaaac agtgataaaa gtggtggttc
     2581 cagaagcgac accagggatt ttgactggtg tgatcctaag ttctggaaga atttttggtg
     2641 aagcagctgc attgatctat acggctggac aaagtgcacc tgcgttagat tttacgaact
     2701 ggaatccatt aagtgtttca agcccgatca gcattttccg tcaagctgaa acattagctg
     2761 tccatatttg gaagatcaat acagaaggca cgatgcctga tggtgtttct gtttcggctg
     2821 gcgcttctgc cgtgttgatc attgctgttt tactgttcaa cttcggcgct cgtgcgattg
     2881 gcaaacgatt ataccgaaaa atgacttcag cctaaggaga agaagcaaaa tgaaaaaata
     2941 taactggaat gaaacaaatg tcatcaccat gccaaaagag aaagaagtag cactatatac
     3001 ggatgatcta catgtctggt atggcgacaa tgaagcaatc aaaggagtgg atctggaatt
     3061 tgaaaaaaat aagatcactg cactgattgg tccttccggc tgcggaaaat ctacgtatct
     3121 tcgttcgttg aatcgaatga atgatgggat cgctaatacg aaaatcactg ggaaaatcat
     3181 gtatcaaggc gtagatatta atgcgcctca agtggatgtt tatgagatgc gcaaacggat
     3241 tggcatggtt tttcaacgtc ctaatccgtt cagcaagtcg atttatgaca atattacatt
     3301 tgctttgaaa cagcacggcg aaaaaaacaa aaagaaatta gatgaaatcg ttgaaacaag
     3361 cttgaaacaa gctgccttgt gggaccaagt aaaagacagc ttggacaaaa gtgccttggc
     3421 tttatctggc gggcaacagc aacgtttatg tattgctcgt gcgatcgcga tgaaacctga
     3481 tatcttgctg ctagacgagc cggcaagtgc tttggaccct atttcgacag gaacggttga
     3541 agaaacactt gtaaacttga aagaagacta tacgatcatc atcgtgacgc ataacatgca
     3601 acaagcagct cgtatcagcg actatactgc ctttttctat ctaggaaaag taatggaata
     3661 tgacaaaact aacaagatat ttaccagacc aaaaattcag gcaaccgaag attatgtgtc
     3721 cggtcatttt ggttaagacg gaggcctcaa catgacaaaa gaaattattt catctaaaga
     3781 tcttcatctg tattatggaa agaaagaagc actaaaaggg atcaatctaa gtttcaatca
     3841 aggggaaatc actgcgatga tcggtccttc tggttgcggg aaatccactt atctgcgttc
     3901 tttgaaccgg atgaatgacc tgattccgaa tgtaacgatc accggaagtg ttttatataa
     3961 agacaaagac atctatggtc caaaaacaga tgttgttgaa ctaagaaaag aaatcggcat
     4021 ggtattccag cagccaaacc catttccttt ttctatctat gaaaatgtca tctatggatt
     4081 gaaattaaaa ggggaaaaag ataaaaaagt ccttgatcaa gtagttgaag aaagcctgaa
     4141 ggcagcgtct gtttgggatg atgtgaaaga caaattgcat aaaagtgctt tatctttatc
     4201 cggtgggcag cagcagcgtg tatgtatcgc acgtgttttg gcagttaatc cggaaatcat
     4261 cttattggat gaaccaacaa gtgcgcttga tcctgtttct actgggaaaa tcgaaagtat
     4321 gctgttagaa ttaagagagc ggtatacgat gatcatcgtg acccataata tgtctcaagc
     4381 ttcacgtatt tcagataaga cggcattctt tttagatggt cacttgatcg aatacaacga
     4441 tacgaagaag atattcatga atccagaaaa acaagagaca gaagattata tttcaggtcg
     4501 atttggataa ggaggaaacg gaatgctacg tacacaattt gaagaagaat tgctgaatct
     4561 ccataatcag ttctacgaaa tggggatgat ggtgagcagc gccgtgcaca agtcagttcg
     4621 tgcatacatc aaacacgata aaatcttagc ccaagaagtt atcgaaaacg atataaatat
     4681 caataacatg gaaacacgct tggagaagaa aagttttgaa atgatcgcat tgcaacagcc
     4741 agttacaact gatttgcgta tgatcattac ggtcatgaaa gctagctcgg atttagaacg
     4801 tatggccgat catgcggtgt ctgttgctaa atccacgatt cgcgtaaaag ggcagacgag
     4861 aataccggaa atcgaaaaag agatctccga tatgtccgat tatgtgaaaa aaatggtgga
     4921 caacgtccta gttgcttatg tcaaaacaga cgaaaaagac gcacgtacga tcgccaatat
     4981 ggatcaaaga gtcaatgact atttcaatcg aatctataat gcaacaatca aaaatatgca
     5041 ggaaaatcct gagacagtca tcagtggaac agactacttg aatgttgcca gttatctaga
     5101 acggatcgga gactatgtga caaacatctg tgaatggatc gtctacttgg ctacagggaa
     5161 aatcactgag ttaaatacga accacaatga gattttctaa actgaagctg tgattatgcc
     5221 gtttgtttag gaaaagaggt tgtgaaatca gcggttgtga aaaagctgtt ctgcatcaag
     5281 taataagaac agtcaaacaa aaaatagctc ctcatatttt tcgtttggct ggacttatta
     5341 ccgcagatgc aagcttttga acaccgttta ttcgggaaag ggtttgtgac aaaacatgtc
     5401 acaaaccctt ttcatcattt catagaaagg ataaagaatg caaaaaaaaa ttagcatcat
     5461 cactatattt tttatttgtg tgcttacttc atgggttatt cagcatcaag ctgctttata
     5521 tgaagtacct gtcatcgaga tcgaaaaagt acgaataaaa gaggatcatc aattgattac
     5581 aggaagactg atgaacacgg atcaaaaagg agaatcgatt tatttatcgg ttccttatca
     5641 aaaaaatcaa atagaaacag caaatataaa agcgagacaa atgtatctag tccaaatgca
     5701 tggagatgaa tggcagctag tagcaaaaaa aaatgatggc tggcttttct tcttcacaag
     5761 tatctccatt ggcgccatgt tgcttattgg cggaaaacag ggtctatttg ctttaggaag
     5821 tctcatcatc aatatcgttt tacttttact tttgttgctc atctttacta aaacagaagg
     5881 gatttctcta ttaaatatat ctatcctgtt cgtcgttata gggatgatta ttagcatatt
     5941 ggctttagat ggatggaata gaagcagtat aatcaaaatt tcagctgcag ttacgagtgt
     6001 ttttttagca ttttttattt gtcttttgac aatgaatcat tggaaagacc aaggactgcg
     6061 ctatgaggaa ctgaattatc tgacacgacc gtatcgttct gtttttcttt ctagcgtact
     6121 gattggaaca gctggaggaa cactcgatac cgttatcaca gtcattgcga cattagaaga
     6181 attgactaag cgacaaccag aaataacagc gaaacagtta tggctttcag gaaggcaagt
     6241 gggtcgagat gtgattggct cgatgaccaa tatccttttg ttcgtctata tcagtgggtc
     6301 agttccatcg cttcttttat atttaggaaa tcaatggtcg ttcaaagaaa cgatagaaca
     6361 gcatttatca ttggaatttt tacgcgtcat tgctggcagc ttaggaatag tattatcgat
     6421 ccctatctcg gtcctctttt ttctgactgt aaggaggctg aaacgatgaa tgtacttatt
     6481 atacttgttg ggattttagc aatcagcgtg ctacttattg gcggtacgca aggtatgtat
     6541 attcttttag gcttattcat caatttaggg atctttttcc tgttgttatt cggttatcat
     6601 cagaagtggc cgatactggc attgtcggtc ataggatttt tattgattgc tgtggttatc
     6661 ttatttttta tcaacggtta taatctaaaa atgcgtgcag cttttgtgag tatactgatc
     6721 tttttatttt gtttcctact attgcttcct atcactgatt ttttagcgat ccaaggtttt
     6781 acatcggtag aactagaaga attatctggc ttagataaga ctttagcgat tgattttaga
     6841 ttacttacga gatcgttgct tctgattagt cttagtggtg ctgttttaga tgctagtgta
     6901 gcaatttcta gtgggacatt cgaagtatat caggcaaatc cgcaattatc tttttaccaa
     6961 ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg cgtcaacagt aatgacactt
     7021 ctttttgcat tcatgggaag ttctttagcg ttgatcctct ggtttattga tttagacatt
     7081 ccttttcagc aagtgatcaa tgaaaaatca tttgttctag aatatacgat ggctattctt
     7141 accacattgt ctgcactact tgttcttcct ttaacctgcg tgactacagc atatctcttt
     7201 acaaaaaaga aagaacaact caagatggag taaaagttac aacttaagtc ctatgacatt
     7261 tttactgaat cagtgcataa tatggatgta aggaaaagct tgcaaacaat tttgcttaag
     7321 gaggcaattt ccatgaaaga aagagaacgt attttagatt tagtgaaaaa aggaatcctt
     7381 tcgactgaag aaggcttaga tttattagaa agcatggcaa ctgaaaaaga cgaaaagcaa
     7441 ataaaaaaag aagcagacaa ggtaactgct agtcataaag aaaaagatca ggcaagtcaa
     7501 ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg agccagttga tccaaaggaa
     7561 agagaaaggc aagatcaaga aaacttagaa aagatcttag atgaactagc aactgaagcg
     7621 aataaaactt cggcgcgtct agatgaagta aatgctcaat tcgctgacaa taaggcagca
     7681 cgcaatgaga aacaggaagc attgatgcag ctgaatacaa aagaagaatt aggcgagttg
     7741 acagaagaag aattagcaca gcgtcagacg ttagaagcag aaatcaaaga attagaagct
     7801 agtggcgata cactgttaga agaacaattg aaattagaag cagaattaaa agatatccgt
     7861 aagaatcaat ggtctgagaa aaaagaaacg tttactgata agtttgaatt gccagatgat
     7921 tggaaagatc aagcaacaga tacactgaat caagtaggcg aaaaaatgtc agaagctgga
     7981 tcacagttag gcaaattctt gaagaaaaca ttccaaactg tttcagaaac agtgaatgac
     8041 aacatggaat ggaaagatgt cagcttacgt gtccctggga ttgcgacaac gaaatttgag
     8101 catgaatttt actatgaagc tcctgcagca agtatccttg atatcaaggc agcaaacgga
     8161 aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag ttgaagctaa gatcaaactt
     8221 tacggaaaaa tgggtgctga accatttgaa gcattctctg aacggagcca gattgaggtt
     8281 aatgaagatc atatctcctt ccaaattcca aataaacgtg tgcgtgcaga ccttgttttc
     8341 tacttaccta aacgagtata cgaccacgca gctattaaac ttctaaatgg caacatcatg
     8401 attgaaacat tggaagctaa agatatttat acaaaatcta cgaatggtaa catcatcgtg
     8461 gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa acggcaatat cgatattcga
     8521 aacgggaaca tcttagattc tattatcgaa acagtaaatg gtacagtgac atttggtgcg
     8581 acagcagaaa acctttctgt gtctttggta aatggtgatg tacgtcttac gattaaagaa
     8641 gataatttga aaaaagtaga agcaagttca gtgaacggaa atgtcaaagt cgctttacca
     8701 gatggcatcg gtttggaagg acatgctaaa acaagtcttg gaagtatcaa cagccgtctg
     8761 tccaattacg aagtagttcg tgagaaaaaa gaacggacaa accaaatgtt gcaattccgt
     8821 cgtgtttcag atggtgaaat cacgcaagtg caactttcaa cgacaacagg aagtatctat
     8881 ttaaaagata cagataaata aagaggttgt gaaagagatg ttcagccttg agcaataaga
     8941 agcgattttg taaaatagct tcccctattt tacaaaattg tgccttattg ccgataggct
     9001 atctcttgaa caccgtttac caagagatta tgaacctgcc aattatcgcc gagtcacaag
     9061 gaataatttc agaaaaagct cgtcacgttt tatgaatatg gtaatttgtg accgagaatt
     9121 ttgcagaaag acaccgatta agaagaaggt tgtcacaacg attttcttca tttaaaatag
     9181 tagaacagag gtgagacatg atgaaaaaaa agttgacaaa atccaataaa aatgttgttt
     9241 tgacagggac tttagcagga ataggagaat acatcgggat tgatccgact gttatccgtg
     9301 tgctttatgt attcctgagc ttagttgctt ttggaagtcc aatcatttta tacattttat
     9361 tagctttgat tatcccatct gcgcgttctg cacgaacaaa ctatggacat aacaatgcat
     9421 attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca aacaaaaaaa cgcaaagaag
     9481 cggaaaaaat cgatgatgac gaatggagtg acttttaatg tcgtactttc aacgtatttt
     9541 agttaatacg ttaacattca tatcattggc tgttcttttt cctgcgatgg tccatgtgag
     9601 cagtatttgg acggccgttc ttgccagctt cgtattgtct gtgttgaata tgtttgtcaa
     9661 accgattttg acgatactgt ccctgccctt tacattagtc acattgggat tgtttacgtt
     9721 tgttatcaat gcagcgattc ttaaaatgac ctcctttttt gtgggagcag aacattttag
     9781 tttttcttca ttcggtgcag cgttgttagt agctatcttg atgtcgcttg tcaatgtaat
     9841 tgtcagtgaa cataacagta gaaaataaaa gaaagctgtg ccgaactatc ggcgcagttt
     9901 ttttatttag aatcaggaat gcgttttcta ttacattggt aattaaataa aaacgttttt
     9961 tttaattggt agtacttttg gctagacaat gataaaatga tagagaacac tctaatagaa
    10021 atgaggtagc atgaatgtca gaagttgtaa aagttagcca attagtgaaa aaattatctt
    10081 tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt catcgtaact ggggacatct
    10141 cacgcccagg actagagttg actggttatt ttaattatta ttcccataat cgtttacagc
    10201 tatttggcag taaagaaatc agttttgcgg aacggatgat cccagaagag cgtttgatca
    10261 tcatgagaag attatgtagt gaagatatgc ccgcatttat tatttcaaga ggattaccgg
    10321 ctccagaaga aatgatccag gcagcaaatg aaaatgggat agctgtactg cgttctccca
    10381 tctcaacttc tcgtttatta ggagaattat ccagttattt agatggtcgc cttgcccctc
    10441 gaacaagtgt ccacggtgtt ttagtggatg tttacggatt aggtgtactg attcaaggtg
    10501 acagtgggat aggaaaaagt gaaacagcgt tggagttgat caaacgcggt catcgattga
    10561 tcgcagacga tcgagtagat gtttatcagc aagatgaatt aacggtgatc ggagaaccgc
    10621 ctaaaatcct ggaacatcta attgaaatcc gaggaatcgg gatcattgat gtaatgaatc
    10681 tttttggtgc gagtgcggta cgtggctcta tgcaagtcca gttggctgtt tatctggaag
    10741 catgggcaaa agataagaaa tacgaccgtc tgggctctga agacacgacc gttgaaatcg
    10801 cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac aggacgaaac gttgcaatca
    10861 tcattgaagt agccgcgatg aatttccgtg cacgtacaat gggctttgac gcaacgaaaa
    10921 catttgaaga aagattaagt cgcttgattg aagaaaactc aggcaacgac tagcaaggag
    10981 tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag cttgtaggga ttcctatcta
    11041 ctggtatgca ttgatcatcg tatcaggtat cattatcgcc atgtggctta gcagcagaga
    11101 ggctgttcgg gtaggattaa aagcagatga tgtgacagat tttatgttgt ggggattgcc
    11161 tatctccatt attggtgctc gcttatatta tgttttattt gatcttcccc agtatatagc
    11221 tgatccaatc caaatattca atatccgttc aggaggctta gctatttatg gcggactgat
    11281 tgcaggcgcg atcacgctgt atttttttac taaatatcat tttatttctt tgtggacttt
    11341 tttagatatc gctgcaccaa gtgtattatt agcacaggca atcggtcgtt ggggaaattt
    11401 catgaaccac gaagcatatg gaccagacac gacgcgaagc ttcttagaaa gtctgcattt
    11461 accgcaattc atcattgata atatgtatat cgaagggatc tatcgtcaac caacatttct
    11521 ttatgaatca ctttggagtt tgattggatt tattttgctt ttgtttttac gaaagaaacc
    11581 acaccttttg aaaaaaggcg aagtcctttt atgttatcta atgtggtata gtttcgggcg
    11641 tttctttatt gaaggattac gtacagacag tctttacttt ttaggtgtga tccgtatttc
    11701 tcaattatta tctgctgtgt tatttgtcgg tacattgatt ctatttattt ggcgcagaaa
    11761 aaaaggaaat ttaccggatt atgaccgctc attaggaaaa aatcaacaaa ttatctgatt
    11821 tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa aatgaattat agagtagcgg
    11881 ttttaggacc aggctcttgg ggtactgctt tggcacaagt cctcgccgaa aacggacatg
    11941 acgttcggat ttggggacat cgtgcttcac aagtagaaga aatcaacacc caacacacga
    12001 atcaacgtta tttaccagat cattatctac ccgattccat ccaagcattc accaatatgg
    12061 aagatgcagt aaaagacgca gatgcggtgc tattcgtcat tccaacgaaa gcaatacgtt
    12121 ctgttgcaaa agaactagtg cctaaattga atacaaagcc agtgatcatc catgcaagta
    12181 aaggattaga acagggaaca cataaaagga tctctgacat cttgttagag gaaatcccag
    12241 cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag ccatgcagaa gaagtagcga
    12301 aacatgacat cactacgatc acagctgcaa gtacagatgc aaaagcagca aaatatgtac
    12361 aagagctgtt catgaataat tattttcgga tctataccaa tcctgatgtg atcggtgtag
    12421 agatgggggc agctttgaaa aatattattg ctattggtgc tggcgctatt cacggcttag
    12481 ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg attagctgag atcagccgtt
    12541 taggtgtagc aatgggtgcc aacccgctga cctttatcgg attaagcgga gtaggcgatc
    12601 tagtcgttac ttgcacaagt gttcattcta ggaactggcg cgcaggcaac ttgttaggac
    12661 aagggcagcc cctagaagag gtgctagcca atatgggaat ggtcgttgaa ggtgtgaata
    12721 ctactcaagc agcgatggaa ctagcccaaa taatggaaat cgaaatgccc atcactcaaa
    12781 caatctatga agtgctgtat gaacaaaaag aaatcaaaac agccgcaaaa gaaatcatgt
    12841 tacgtgatgg gaaaaaagaa aatgaattct cattaacaat tttttgaagg agcgaatgta
    12901 tatgaaagtc aaaaaagcag ttattccagc ggcaggatta ggaacaagat ttttaccagc
    12961 aacaaaagct atggcaaagg aaatgttgcc aattgttgat aaaccaacaa tccagttcat
    13021 cgttgaagaa gcgttgaaat caggaatcga agacatcttg atcgttacag gaaaagctaa
    13081 acgaccaatc gaagatcact ttgattcaaa tattgaatta gaaacaaacc taagtgaaaa
    13141 aggaaagacc gaattattga aattagtaga agaaacgaca gatgtcaact tgcatttcat
    13201 ccgtcaatct catccaaagg gtttaggaca tgccgttctt caagcaagag cctttattgg
    13261 aaatgaaccg tttgtcgtta tgctaggcga tgatatcatg gaagatgaag taccattgac
    13321 acagcaatta atcgatgatt acgaacgtac tcatgcttct acgatcgccg ttatgcaagt
    13381 tcctcatgat gaaacttcaa aatacggaat tatcaatcca ggagaagtat tggaagatgg
    13441 gttatacaat gtcaaaaact ttgttgaaaa accagatcca agcaaagcac caagtgattt
    13501 agctattatt ggacgttact tattgactcc agaaattttt gatgtattgt ccgagcaaca
    13561 accaggagcc ggaaatgaaa tccaactaac tgacgcaatc gatacattga ataaaacaca
    13621 acgcgtattt gcccgtaaat tcacaggtaa acgttacgat gtcggtgaca aatttggttt
    13681 tatgaaaaca agtattgaat atggtctaac acatccagaa gtaggtgcac ctttacgtga
    13741 gtatatcctg tctttaggta aaaaattaga agcagaagga agcaagatcg atcataatcc
    13801 aaaaggaata acagaaatca aagaagaaaa gaaaaaataa cccttttttc taacgagctt
    13861 cagctgctaa ttgaataaaa agtagttgaa gctcgttttt atttgtttaa aaaacacgaa
    13921 aatgctatcc ttatagaaaa gagagacaag aagggagttt gatcatgtca ggaggagaaa
    13981 ttgcaggatt gatcgcagca atcgcctttg cgatacttgt cgtttttatc gttcgtgtgt
    14041 tgttgcaagt ttctaaaact ttagaaaaag tagatcagac tgtagcagaa gcaaatacga
    14101 cgatcgaaat cgtcacaaaa gacgtagatc ttttgtctag acaagtagaa ggcttgctgg
    14161 tcaaaagtaa tgaactgctc ttggatatca atggaaaagt cgcaacgatc gatccattgt
    14221 tcacagcagt tgctgattta agtgaaagtg tatcagaact taatcattct agtaaaaaca
    14281 tcgcgacgaa agtcggcgga atcggtaaaa cgacagctaa agcgacggtt gccggaaaag
    14341 tcggtgggac agcgatgaaa ttttttaaaa acaaaaaaca tactgaaacg acaggaggaa
    14401 aataaaaatg gctaaaaaag gtggattttt attaggtgca gtagtcggag gaacagccgc
    14461 agcaatcgcc gcgttattgt tagcaccaaa atctggtaaa gaacttcggg atgaattagc
    14521 tgcccaagcg gatgatttga tggatatggc ttcagattac acagatatgg cgaaagaaaa
    14581 atcgactgag ttaacaggta ttgcaaaaga aaaagcgcaa gatctttctc aacaagcagg
    14641 tgatctagca ggtagtgtca aacaaaaagt aagtgaatca gcagatgaag caggaagtga
    14701 atctgacgat atcttgttaa atcttaaaga acaatcagct gacttatcta atcgtttcaa
    14761 aaaaacggca gaagatatga aaaaacctgc agatgaatta ggaagtattg ctaaagatac
    14821 ttcagaagat atcatgattg atgtaaaaga cgctgctggt aaagcaggag atactgtcaa
    14881 tgaaggaatg tcagaagcaa aaccagttgc agatgacaca aaagaaatga tgaaaatggc
    14941 aaaagaagat gcgaaagaag ctaaaaaaga aataaaagat acaatgacag attcatctac
    15001 aaatcactaa aatcactaaa atcacaaatt tatagaataa aaaggtggga gcggactagc
    15061 tctcaccttt tttactctta tttttctaaa actagtaatt cttttggtgt agtcgtgaat
    15121 ggcacacagc catcgcttgt cacatataca cagtcttcga tacgtacccc tactttttca
    15181 ggaatataga ttcctggttc aagagagaag cacatccctt cttcgattac taagtcattt
    15241 ccttctacaa gggaagggaa ttcgtggacg gttgttccga tcccgtgacc taaacggtga
    15301 ttgaaatatt cgccgtagcc atagctgttg atcacgtttc gtgcgatttg atcaagttca
    15361 cctgctgtca cacctggacg gacggcttct tgtgcggcaa gctgtgcttc aagagtgatg
    15421 ttgtatattt tttcttggaa gtcggttggt ttttgataag caattgtacg agtagcatca
    15481 ctgcaatagc cattccaaac aacgccaaga tcgaataaca caagttcatt cggtgaaatc
    15541 ttcgtgtttc ctggtgtacc atgagggctt gcagcatttg ctcctgcaag gactaatgtg
    15601 tcgaaagaca tttgtgaaac accttgtttt ttcaattggt attcgatttc tgcaatgatc
    15661 gcttgttcag tcacgccttc tttcactgct ttaaagccaa tctcaaatgc aacatcggcc
    15721 catttaccag cttcgatcag tttgtctttt tcctctagag ttttgatcag ttgtagacgt
    15781 tggatgatgg aagctagatc agtgtaagaa tccgttcccg tcaagatatc agagatagca
    15841 tcgaagcgct cgacagtcaa ggcgttcttt tcaatcccta cattgccagg ctgaccatag
    15901 cgagcacgaa gttcttttcc gatgatctcc caagggtttt cgctgtctaa atagccacgg
    15961 acatcatacg cccaactact gttttgagca tcttcaactt ccaaagcagg agtgaaaagg
    16021 aaaggatctt gttcaagagg gataaaaaga gctagtacac gctcatgagg atcactttcg
    16081 tatccggaaa agtaagcgat gtggccaggg tcgctgatat aagccaattc gacttggtgt
    16141 tgtgccatcc attgacgtag ttcattgatt tttacgttat tcatttggca acctactttc
    16201 taaattaatt ttgtattcaa taattgtacc actgatttat taaaagacca acgttctaaa
    16261 aactgaaaaa agtaaaaatt ttcatgaaaa cagagataaa acggttgcaa ttcttgagat
    16321 tatttgatat tctattttag aaatgtaaga cgtattttca gaaaacatat aaaatatgaa
    16381 taggagaaca caaatggaaa agcaaacaat cactatctat gatgtagcga gagaggcaaa
    16441 tgtttcgatg gctactgttt cacgtgtcgt aaatggcaat ccaaacgtca aaccagcaac
    16501 aagaaaaaaa gtcttggaag ttatcgatcg cttggattat cgtccaaacg cagttgcgcg
    16561 tggcttggca agtaagaaaa caacaacagt tggggtaatc atccctgatg tcagcaatat
    16621 gttctttgct tctttagcac gtggtatcga tgatgtggca acaatgtaca aatacaatat
    16681 tatcttagca aattcagatg gtaacgacca aaaagaagtc aatgtattga ataacttact
    16741 ggctaagcaa gtggacggcg tgatcttcat gggtcaccat atcacagacg aaatccgtgg
    16801 agaattttca cgttcaaaaa ctcctgttgt attagctggc tctattgatc cagatgaaca
    16861 agtaggtagc gtaaacattg attatgtatc agcaacaaaa gatgcagtaa acaaattggc
    16921 aaaaagcggc aataaaaaaa tcgcttttgt cagtggtgcg ttgatcgatc ctatcaatgg
    16981 acaaaatcgt ctgaaaggct ataaagaagc attaagcgaa aataacctac catatagcga
    17041 aggattgatc tttgaagcac aatacaactt caaagatggt ctatctctag tcgatcggat
    17101 ccataatagc ggtgcaacag ctgtctatgt ttcagatgat gaattagcca tcggtatttt
    17161 ggatggttta ttagaccgtg gcgtgaaagt accggaagaa ttcgaaatca tcacaagtaa
    17221 caattcacta ctgacagaag tagctcgtcc tcgtttaaca agtatcacac aaccattata
    17281 cgatttaggg gcggtatcta tgcgcctatt gacgaaaatg atgaataaag aagaagtcga
    17341 agaaaaaacg attatcttgc cttatggaat cgaagaaaaa ggatctacaa aataataaaa
    17401 ggacaaccat tcagaggttg tgagcctgcc gtttaactct gcgttacaag gagcaatttc
    17461 aaaaaacagc atttcatgtt tgatgaatat tgtgactcgt gattcggagt tggcaggaga
    17521 acaccgttta ttcgggaaaa
//
LOCUS       DAYYHX010000043        17522 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000043 DAYYHX010000000
VERSION     DAYYHX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.043"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..1030
                     /locus_tag="ACHA6Y_001067"
     CDS             74..1030
                     /locus_tag="ACHA6Y_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8987179.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1103..1219
                     /locus_tag="ACHA6Y_001068"
     CDS             1103..1219
                     /locus_tag="ACHA6Y_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM8987180.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1274..2956)
                     /gene="recN"
                     /locus_tag="ACHA6Y_001069"
     CDS             complement(1274..2956)
                     /gene="recN"
                     /locus_tag="ACHA6Y_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM8987181.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(2974..3423)
                     /locus_tag="ACHA6Y_001070"
     CDS             complement(2974..3423)
                     /locus_tag="ACHA6Y_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8987182.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(3530..4348)
                     /locus_tag="ACHA6Y_001071"
     CDS             complement(3530..4348)
                     /locus_tag="ACHA6Y_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM8987183.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(4357..5247)
                     /locus_tag="ACHA6Y_001072"
     CDS             complement(4357..5247)
                     /locus_tag="ACHA6Y_001072"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8987184.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5249..5473)
                     /locus_tag="ACHA6Y_001073"
     CDS             complement(5249..5473)
                     /locus_tag="ACHA6Y_001073"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM8987185.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(5478..6815)
                     /gene="xseA"
                     /locus_tag="ACHA6Y_001074"
     CDS             complement(5478..6815)
                     /gene="xseA"
                     /locus_tag="ACHA6Y_001074"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM8987186.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(6816..7670)
                     /locus_tag="ACHA6Y_001075"
     CDS             complement(6816..7670)
                     /locus_tag="ACHA6Y_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM8987187.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(7767..8216)
                     /gene="nusB"
                     /locus_tag="ACHA6Y_001076"
     CDS             complement(7767..8216)
                     /gene="nusB"
                     /locus_tag="ACHA6Y_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM8987188.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8209..8634)
                     /locus_tag="ACHA6Y_001077"
     CDS             complement(8209..8634)
                     /locus_tag="ACHA6Y_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8987189.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(8880..9944)
                     /locus_tag="ACHA6Y_001078"
     CDS             complement(8880..9944)
                     /locus_tag="ACHA6Y_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM8987190.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(10116..10361)
                     /locus_tag="ACHA6Y_001079"
     CDS             complement(10116..10361)
                     /locus_tag="ACHA6Y_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8987191.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(10500..10874)
                     /locus_tag="ACHA6Y_001080"
     CDS             complement(10500..10874)
                     /locus_tag="ACHA6Y_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM8987192.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(10957..11871)
                     /locus_tag="ACHA6Y_001081"
     CDS             complement(10957..11871)
                     /locus_tag="ACHA6Y_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8987193.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(11892..12695)
                     /locus_tag="ACHA6Y_001082"
     CDS             complement(11892..12695)
                     /locus_tag="ACHA6Y_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM8987194.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(12728..13717)
                     /locus_tag="ACHA6Y_001083"
     CDS             complement(12728..13717)
                     /locus_tag="ACHA6Y_001083"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8987195.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(13820..14311)
                     /locus_tag="ACHA6Y_001084"
     CDS             complement(13820..14311)
                     /locus_tag="ACHA6Y_001084"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8987196.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(14502..17309)
                     /locus_tag="ACHA6Y_001085"
     CDS             complement(14502..17309)
                     /locus_tag="ACHA6Y_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8987197.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
BASE COUNT         4963 a         3891 c         2904 g         5764 t
ORIGIN      
        1 cttacaaaca cattccatgt attccaattc cctatccgtt ataataaaag aaacaggcaa
       61 caggaggaaa atcatgatca aatattttac attagataat gagaaactgc gtaataccgg
      121 aaaggaaact gaccaaacga tctggtattg tgtagaacgt cctaatcaag aagagattca
      181 tcagctgaca catcaatttg aaattccgtc agattacatc acttccatat tggacgatgc
      241 agaaaattcc cgtacagaag aattcaatca atcaaagttg acaaagccag cgttattatt
      301 gttacaatat ccttttgcta aaacaagtcc tagcggttat tttcaagtgg atacataccc
      361 gctctctatt attttgacgc ccaaaaagaa gcttatcaca attacgaatc atgaaccact
      421 gtttttaaag aaagtcttta gccagacctt tccaaaaagt gatatttcgc ctaatctcaa
      481 tatcttcttt caattcattt ggcaggtcac actctcttac aacagttatt tagctacgct
      541 tcaatctcaa tgcgatactt tggaagccaa acttcaagta tccactgaaa acaaacaact
      601 ttatcaaatc atggatatcc aaaaaagcct cgtttatttc agagaagcga cttctgctaa
      661 tttagatgca ttgaaagaac tagcgcaaaa taaacttttc caagaaaatc atgctttgaa
      721 aaatcattta cgtgatgtat tggtggaaac agagcaggca atgactactt ctcgcattca
      781 attaaaacta gtagaacaaa tgaatcagac tttttcagca atcgtatcga ataatctgaa
      841 taatattatg aaaattttaa cttctttaac gattatttta actataccga caatcgtcgg
      901 cagtatctat ggaatgaaca ttaagttacc tattgctgaa agggatgatg cctttatatg
      961 gctagttcta ttaacattat ttatttgtat cgtaactacc tattatttaa aaaaagggaa
     1021 gtttttataa aaaagccctc ttgtggaaat ggaaaaaaga ttgtacactt gaatcaacta
     1081 attaatgacg aggttgaaaa caatgcgtga aaaaaaacaa acaagtactt ttgctaaagt
     1141 aacaaaattt gtcatttgga caatgctgat tttaacgatt ggatctgttg tattgactgc
     1201 tgtaatgagt gtcatgtaaa aataaaatat agctatcagc tgctgaaaag gacctgaacc
     1261 actgcgaggt ggctcaggtc ctttttgcga gtttcaatag ttcttttgca tgttcgagtg
     1321 tgagttttgt aatctcactt ccagccagca tacgtgcaat ttcttcttct ctttcttttg
     1381 ttttcaactc tgaaacagaa gtttgcgtac gtccatcaat cactgctttc acaatataat
     1441 attgatagtc ggcgactgca gcaacttgag gcagatgagt gatgcacaaa acttgagagt
     1501 atttcgatat ttgagaaatt ttatctgcga tggcctgtgc aactcttccg ctcacacctg
     1561 tatccacttc atcaaaaata atacttgtca caccttgctc agaagaaaaa atagttttca
     1621 aagcaagcaa catccgagac agttcgccac cagaagcgac tttcaccaaa ggcttcagtg
     1681 gctctcctgg attggtcgta atgtaaaatt cgatttggtc aaatccatca tgattcaatt
     1741 gtctattttc ttcttttaag aaacgtacct caaactcagt gttttccata tacagacttt
     1801 tcaattcatg cagaatggat ttttccaatt cttttgcgat ttttttacgc gcttcatgaa
     1861 gatctgcggc ttgttgctga agtagaagct ctttttgctc tagttcctct ttcattttgt
     1921 ccaattgacc ttctgaaaaa tcagaagaag ctagctcttc ggtgatttcg tcatagtaag
     1981 ccaatattga ctcaatggat tcgccgtatt tacgtttcaa ctgtcggatt aattctaaac
     2041 gttgcgtaac ttcttctaaa cgactctcat ctaactccaa taaatcaatc tgacgactca
     2101 tatcgccgat tgcatcttgc agcaaatagt aagcactttg gatattttca taaattgctt
     2161 catactccac atctagatgg gcgatccctt gcagttctga aacggccaag ccaacaccat
     2221 ctacactgtt ttgttcatta ttcgtcagtg ctccgtaacc tgcagctaat ccatcagcaa
     2281 ttttttgata attgctaagt ttttctcttt cttcttttaa tttctcttct tctccgactt
     2341 gcaaatcagc agcagctatt tcttcttgct ggaaatggag catatctatt ctctgaacat
     2401 aggatttttc attttgctgc acttttcgta cccttttttc cagctcacga taagagatat
     2461 attcttgctg ataactcttt ttcttctgtt ggaaagctac atctccaaaa cgatccaata
     2521 aagcaagatg tgattcaggc tggagcagtt cctgatgttc gttttgtcct tggatatcta
     2581 ctaaataact gccgattctt ctcagattag ctaatgtgat aatatgacca ttcactcgac
     2641 aaacattttt tccagttaag gacatatccc gtcgaacaat cagattgtcc tcatctgttt
     2701 cgatccctaa ttcaaccatc agttcagaga atccttcttg ttttggtagt tcaaacaacc
     2761 cttcaagcac acatttttct gctccttgac gtatataatc gcttgaacct cttcctcctg
     2821 ctaacagacc taaagcatcg ataataatgg atttacccgc accagtctct ccggtcagcg
     2881 cggtcattcc ttcgtgaaaa gaaagccgca attccggaat aatcgcaaag ttagtaatac
     2941 tgatttcttg tagcatcaca ttcgctcctc aacttaaagg taatgcagga agtctttctt
     3001 caataaaact gcctggtctt cagttcgtgt aatcatcaaa acattatcgt catcattgat
     3061 aatcgaaaat aattctttct tgtaacgttt atcaatcaaa tttgctgctg ccgaagcatt
     3121 tcccggcaat gttttcaaaa taacaaattt ttccatttgg tcaactgcca cgaaagcgtc
     3181 ttttaatagt ttttccaatt tagtatttac atcttcgttt gtttcagctg gtatgctata
     3241 acggtagccg ccatctgaag ccggtacctt gatcagtttc agttccttta tatctctgga
     3301 aatcgtagct tgagtaacag atattccttt gcttttcagg atttcaacaa attcttcctg
     3361 ctttcggata tcttgctcac tcaacaatcg agtaatcaaa cgatgccgat ctttttttct
     3421 cattacgtct tcgctcctta gcatattctt cttaatcaat gatagcttat ttccttggta
     3481 taataaagaa gaatccgcat acttctcact tgattttcac agttacaatt tatttatggt
     3541 caaactgttc gtgtgcattt gaaaccactt ctgccatatt aatagctgaa ttgattgttc
     3601 cgctttcggg tactttcctc aagtgagcca agaactcgat atttccttct ccccccgtga
     3661 tcggagaata acttaattcc agcacatcat atccaccttg tgccgcaaat gacaaaatgt
     3721 cttctaaaac tttttgatgg gtctgagggt cacgaacaat tccgtttttc cctacagctt
     3781 ctctgcctgc ttcgaattgg ggtttgatta aagcaatcac ttctccctct tcttttaaaa
     3841 tagcatgtaa tggaggcagc atcagtttta gtgaaataaa agaaacatcg ataacagcaa
     3901 cttctggaat tcctaaggta aagtcttcgg gttttgcata acgaaaattc actctttcca
     3961 ttacgacgac tctaggatct tgtcttattt tccaagctag ctgattgtag ccaacgtcta
     4021 aagcataact catttttgcg ccattttgca aagctgcatc agtaaatccg ccagtggatg
     4081 caccaatatc taacaatgtt tttccttcaa cagatagatc aaatgctttg agtgcttttt
     4141 ctaattttaa tccccctcga gaaacataag gaagtttttt acctttgact cttaattcac
     4201 tactcactgg aattttttct cccggtttat caaaccgctc gtttttttcg ttgtaaatca
     4261 aaccagccat cactgaacgt ttggcttgtt ctcttgtttc aaataatcct tgtttgaccg
     4321 ctaatacatc tacgcgttct ttttccattc tttccctcat ttctccacat caaattgctt
     4381 gatcatctgg cgcatcagcc cgccatcaaa agaaaaaacg tcctcttcta atttgtctat
     4441 gatcttgttg gcggataaca gctcaatctc caacgcctca cgtgtctttt ctaggcctaa
     4501 taaacgcggg taagtatttt tttccatacg ttcatcttta ccagctgttt tccctaatgt
     4561 tttcgttgta ccaatcacat cgagtagatc atctcttatc tgaaaagcta aacctaaatg
     4621 ttcggctaat cgttgaagta gcaataagat ttcttcaggt tgcttcgcta atatgccacc
     4681 agccagcaag gcataacgaa tcaaatgccc tgtctttctt tcatgaataa atgctaattc
     4741 ttctaaagaa agctttttcg attcgccctc gatatctgca gcttgcccag caaccatacc
     4801 ttgtgatccc gcacaaacag ctaattgctg aagcagcaga agcttaggag aattacctaa
     4861 gtgtgccata ctgattagct ggaatgctcc tgtaagcaaa ccatctccgg ccaatatagc
     4921 taatgcttcg ccgaatactt tatgattggt aggttttcct ctacgaagat catcattgtc
     4981 catcgcaggc aaatcatcgt ggatcaagga atacgtatgc accatttcta aagcagctgc
     5041 tacttgatag acgggaacat caataggctc atcaaaggaa gcaacggccg ttaaaagtaa
     5101 taagggacgt attcttttcc cgcctgcttt cactgaataa ctcatcgcat catagagtcc
     5161 tctatccgtt gtatgctcat ctaaaaaaga caagatttct ttttctacag caggtagatg
     5221 aatagctgaa aagtcagtta atctcatctc agctttcctc gtttccgtca aaatcaactt
     5281 cttcatttga ttctgtcatc attttcgtca aggtctcttc tgctttagct aatgtatctt
     5341 ggcaatgttt gcttaattcc attcctcgtt taaacgaatc tagagctgat tctaatggaa
     5401 cgtctccttg ttctaactgc atgacaattt tttctaattc ttgcagtgac tcttcaaaag
     5461 ttggattttc catattttta ttctcctttg tctatctctt gtacttgtcc tttaacgatt
     5521 ccatcttgat aatggatcgt cagcaaatcc ccttgatgaa tctcggtgac actttttact
     5581 acttgatcat ttaacgtcgt atagctgtag cctcttccca tgattttcaa tggactcaac
     5641 aaatcaagag attgaacagc ttgagcaaaa cgctgctgct gttgtttcat atattgttgg
     5701 attttctctt ctaagcgttt ttctagataa gcagtttctt ggcgagctgc tttaactcga
     5761 taaagcggag ccacttgttc caattggtgg cttaatgcga tcgtttgttt ttctttcgca
     5821 tgataaattt gctgtgtagc ttgatataat cgctgtttca actggtctaa tttgatggtc
     5881 tgaccttcat acaatctttc aggctgccga aatacatagg aacgtcgagc acgctcgaac
     5941 cgttcttgtt tgcgctggat ctggcgtaca aacgcctgct gcaatctagc ttgccgttct
     6001 tgaatgcgca tcaactcttc tgacaaaaca ggaacagcta attctgcagc tgctgttgga
     6061 gtggcagccc gaacatctgc tactaaatca gcgatcgtgg tatctgtttc atgtcctaca
     6121 gatgaaatca ccggcgtatt agcttgataa atcgcccgag caacccgctc ttcattaaac
     6181 ggccataaat cctcaatcga accaccccca cgaccgatga tcattgtatc gaaattgccc
     6241 atctcttcta ctctctggat attcctgaca acattgtccg ctgcttggtt tccttgtaca
     6301 actgttggga acaaaaccaa ttggacaatt ggatatcttc ttttcaccgt cgtgatgata
     6361 tcgcggataa ccgctccgct tggactggta aggaccgcga ttcgctttgg gaaacgtggc
     6421 agcatttttt taggcccttg aaaaaggcct tccttcatca gcttttcttt tcgttcttcc
     6481 aattcttgat ataaagcacc tattccatca ggctccatgt gctctacata gatttgataa
     6541 gaacctcccg cttcatataa tgaaatacgc ccaacgacaa ggactttcat tccttctttt
     6601 ggctggaatc gtaatttttg gaatgctcct ttgaacataa tagcagatat ttttgcatgg
     6661 tcatctttta agctgaaata ttgatgggca ttggcacgta aacggaaatt cgatatttcg
     6721 cctgttaaat atacgcgttc taaataagga tcggcatcaa attttctttt caaatacttc
     6781 gttaatgtag tgacagttaa atattcttgt gccatctatt cacctgctcc aaaagcttcg
     6841 gcagatttta cggtttgata catcagcatc gtgatagtca ttggccctac accctttggc
     6901 acaggagtga tccagcctgc aataggctcc acttcatcaa atttcacatc accgatcagc
     6961 ttaccgttag catcacgatt catccctaca tctatcacta cagcacctgg ttttacgaat
     7021 tcttttgtga caaaatgtcc acgtcctatt gctacgacta aaatatctgc ttcttgtgcc
     7081 agttcagcca gattttcggt tttagaatga gcaacagtaa cagttgcatc tgccatcaag
     7141 agtagctgag ccatcggttt accgacgata ttgcttcgtc cgataactac tgctcgttta
     7201 cccgccggat cgatagaata agcttcaaac attttcatga tgccgtatgg agtacaagga
     7261 atcttgtccg gtttacccgc aaggagtctg cctaaattca tcggatgaaa accgtctacg
     7321 tctttttcag ggcggatagc taacaggatc ttttcttcat ctatatgctt tggcaatggt
     7381 aattggacca atataccatg aaaactagga tcttgattgt atttttcaat ttctgcaagt
     7441 agttcgtctt cagaaatcgt ctcaggataa cgctctacca atgaatggat tccgatttct
     7501 tttgctacac gttctttatt tcttacatag atttggctgg ctggattttc ccctaccaat
     7561 aaaacgacta atccgggatg gataccatta tctttcaatt tttggatttt ttcagcaatc
     7621 tctgcctgca ttttttcagc taattctttt ccgttaatca attctgccat ctaaccactc
     7681 ccagatattt tttgtgtttt cattctacca tagaattttc ccgatttata acaggtttat
     7741 gatgattcat agaaaaaagc taggacttac gccccagctt ctaattcttt caatacattg
     7801 gataaaacac cgttgacgaa tttacgtgaa cggtcatcac taaatgtttt tgccaactcg
     7861 attgcttcat ttaaagcaac agccgctggt acatctgtca cataagtcat ttcaaaaatc
     7921 gcgatacgca agatcaccaa atccattttt gagatccgat tgatgtgcca attgtttttt
     7981 aaatgttttt caatcacttt atccaattca tctttttttg cacagacacc gccaactagc
     8041 gtatcaagat aaacaggaac aaactctgat tcatcttcgt tgatcatgtc gcggtggtct
     8101 aattcgattg cgttgaaaat cgcatcttct tttgttaggt ctgcattgaa gtctaatgga
     8161 aataaagcct gcaatgccat ttcccgaatc tcgtgtcttg ataattcttt attcattctc
     8221 ttcctcttcg tcttcaaaaa gttcttcgat tgttggttgt tctaattttt caggaacaac
     8281 agctactaca tggatattga cttcagataa atcgatatcc gtcatgaaaa ggacttgctg
     8341 gctgacccgt tcctgcattt ccattgctac tttaggaact gaaacaccat attctagata
     8401 acagtaaaga tctactttca agccatcttc ctcgtttgct aagtaaacac cttttccgtg
     8461 agctgcacgt cctaataatt cagtgacatt gctggcaaaa gttccgcgca ttccgtatac
     8521 gccttctact ttagaagctg caatcccgat aattacttcg atgacttcag gagcgatcac
     8581 gatctctcct aaatcttggt ttgcgtttaa aatcaaattt ttttcatctg ccatttattt
     8641 attcctccct ttgcctaagc acgagataca tccgaattct ctatgatgcg gcatagtgat
     8701 cacgtggtaa gaaaactgcc gccgatcagc tgcaaactgc tatgaattgg agaaaacacc
     8761 caactttatt atctcttaca gcttaaaagt tcttctatag tgtatcatta aatcgagtga
     8821 ttttctataa caaagtctgt gaacactgat ttttacgctt acttcttcta ttgatgaaac
     8881 tataagatga tcaattcttt tggtgaacga gtcaacacac gatttccatc ttctgtgatc
     8941 aacaagtcat cctcaatacg tactccaccg attcctggta agtagatccc tggttcatca
     9001 gtgatcacat ttcctggtac aaattgcttt tctgcacgag aagaaacgtt tggtccttcg
     9061 tggatctcta aaccgatccc atgtcctgta gaatgtccaa atgcttcccc gtaaccatgt
     9121 tttgaaatat aatctctagc aactgcatct aattggacac cagtcattcc tggttttgca
     9181 gcatcaataa ctgctaattg agcatctaag actatttgat aaatttcttt taatttttcc
     9241 ccaggatcac caatagcgaa tgtacgtgtc atatcggaaa cgtacccttc gtaatagcaa
     9301 ccaaagtcca atgtgatcaa gtcaccttgt tcgatgattt ttttgcttgc tacaccatgc
     9361 ggcatagccg aacgcaatcc gctagcaacg atcgtatcaa aagaaacacc tgaagcgcca
     9421 agagaacgca tataaaagtc tagttgattt gcgacttcaa tctcagtcat gcccggttgg
     9481 atcattttca ggatgtgatc atatgccata tccgcaatat gacatgcttt ttcgataata
     9541 gcgatttctt cttcatcttt cacttctctg agttcttcga tcatctgtga aacaggtact
     9601 aattccgcat cgatgatttc ttctaaaacc gaatattcta aaaatgagac tgttgtttcc
     9661 tcaaaagcta atgagtgcag ttcttctttg cgtactaagt ttgctacctc atcaaaaata
     9721 ggtccgacat ttttaataat ttcaaaccct tgagcctgcg ctgccgcctg ctcggtataa
     9781 cgaaaatcag taataaaaaa tgctttttcc aaagtaataa cagctaatcc tgtcgtacct
     9841 gtaaagttcg ttaagtaacg aagattgtac ggactggtaa ccaaaaatga atccaaattt
     9901 tcttcttgca tttttttgcg caatttttct acacgtagca tcataatgaa actccctctt
     9961 tctttgcacg cttttttcca ttatagcaaa cttttcttcg tacacgacag ttaaataaaa
    10021 atttccggaa aacttctttt tattttaaat aaataaagcc aaaaaggagt cacagtctcg
    10081 ctgcaactcc ttttagttta ctattttgta aaaacttatg atcgttttcc aaagaaaaat
    10141 gagacaactg ctaccaaaat cactgcgcct aaaatagatg gaataagtgc catacctgca
    10201 gcttttggtc cccattcgcc gaaaatcgct tgtccaatac ttgaaccgac tagtccagca
    10261 atgatattcg ttacacagcc catcgaattt cctttactgg tgatcgcacc tgcaattgct
    10321 ccgatcacgg ctccaacaat caatgtccat aaccaactca taatataacc tccttctttg
    10381 ctaatcaatt ttagcatatg ggatttaaag gggacaaata attggttttg ctatttcata
    10441 ttttcttctg caaagtaaaa aaataaagaa aacctcctct tagaagtttt ctttattttt
    10501 tacttgcgtt taaacatact acgaatacca cgtttcacta catcaaagaa actcaatgat
    10561 tgtaccattc tatcttcatc tacatattct ctgatcgcac gaagcacttt tttaggttct
    10621 ttggaagaaa atgtgtatgt accatttttt ttcgtttgaa tggcatatcg aggaatccat
    10681 tttcccttta gcatgactga tgctagtaca taatccactt cttcccatgg aatttggata
    10741 tatttgcgtc tatctcgcgc atggtaaaac tcaaaaccat tattacctat catgatttgc
    10801 ccgtaatctg tcagacccgt aaacgaagta gcatcaatta ccaagtctac ttttgtattg
    10861 attgattgaa ccattttctc cctccagata tttgtttatg aacagtatac tatataaaaa
    10921 aagctcggga caacgcccga gctatatttt tatggattat aaaagtccga tgacgtgtcc
    10981 aacaacaccg actacgaata aaccaaggat gataacgatt ggagaaacct ttttcttaag
    11041 caaccacata cataacaatg ttaatcctaa tgcagctaaa ccaggaatca attggtctaa
    11101 gttgtcttgt aatgttgtta ccttttcagg agaaagggct aagccattat taacttgttc
    11161 aaatgcactc ttgattcctt caccacctga aggcaattta tcccattcga tgtaagcacc
    11221 tttatctaat tttacagttg atacaactgg taagaatttg attgataccc atctttgtac
    11281 aagggcagct aatacgaaca tccctaaaat tgaagcacct tttgttacgt cttgtaacaa
    11341 tccgccagaa aggtcatcag taattttaga accagctttg tagccaaatt cttgtgtata
    11401 ccacatgaat gcccaacgaa taatgttcca acctaagaag aagatgattg gtccaaggat
    11461 atttcctccc atagctaatg aagcacctag tgcacctagc attggacgta ctgtaaacca
    11521 gaacactggg tcaccaacac cagctaaagg acccatcata cctactttaa ccccttgaat
    11581 cgctacatca tcaacaggcg cgccattcgc acgttcttct tccagagcca atgtaacccc
    11641 taaaattgga gaagcaatgt atgggtgtgt attaaagaat tccaagtgac gttttaatgc
    11701 tgcagaacgt tcttcttttg ttttgtataa ttttttgatt gctggaatca tagaaaatgc
    11761 ccagccaccg ttttgcatac gttcgtagtt ccatgaacct tgaatgaagg tagagcgcca
    11821 cgcaacagct aaacgatctt tttttgataa tttgattttt tcttctgcca ttttattttc
    11881 ctccttcaag attaatagtc attcaaaatg tcgcctaaag ggtcaccaga gttgcttccg
    11941 cctccattat ttgatgaacc acccatttta gaaaggttca agtaaataag agctaatgca
    12001 acacctaaag cacctagagc aatcaatgtt aattgagaaa ttgcagctac aacgaaacca
    12061 ataacgaaga atggccatac ttcttttgta gccatcatgt tgattaccaa agcgtaacct
    12121 acagctacga ccattccccc accgatagcc ataccatctg ttaaccatgc tggcattgat
    12181 tcaaggaatg attgcacagt ttgtgctggg ataaatagca acgctgctgc tgggaccgcg
    12241 atacgaaccc cttgtaaaca tacagcaatg atgtgccaca tttcaacctt acggatgttt
    12301 ccttcttcag cagccgcatc catcaaatga acgattggaa cagcgattgt acgtacgatc
    12361 attgttaaga acaaccctgc aacagccaaa ggaacggcaa tcgcgatcgc agaaggaaca
    12421 cctttaacac cttgtccacc taaaactaaa ataattgctg atgctactga tgctaatgca
    12481 gcatctggtg ctacagcggc accgatattt gcccaaccta aagcgatcat ctgaagcgta
    12541 ccgccaagta cgataccagc ttcgaggtta ccggtaacta aaccgatcaa tgtacatgcc
    12601 actagtggtt gatgaaattg aaattcatca agaatacctt ccataccagc tagaaaggca
    12661 actaatacga ctaaaattat tgaaataata gacatgataa gcctcctata aattttaata
    12721 ttatttatta cgcttttgct aattcagctt ttgctttttt caaaatatca tccatgttat
    12781 cgcgtgaatc attaggtact ttacgtacat cgaatttcac acctttttgt tcaagcttct
    12841 caaacgcttc aacgtcttct tgtcccatag acaatacttt acttacaacg actttaccga
    12901 ccgaatgcgc cattgaccca acatttaatt ctttgatgtc tacgccacct tcaacagctg
    12961 tcaatacgtc ttcaggattt tcaaataaaa gcaatgcttt ggtatttcca aaacgagggt
    13021 cttttgctac ttcgatcatt ttgctaatag gaataacatt tgcttttaca cctggaggag
    13081 cagcttgttc gattagtctc ttacgcagat catcctttga tactgcatct gaaacaacga
    13141 taatacggtt tggttgcact gcttttgtcc aagctgtcgc aacttgtcca tgtaacagac
    13201 gtgagtcaac acgtgctaat acatatttga tttttccgtc tccgaccaca gttccttctg
    13261 gaagtgctcc tttaggttgt gcgtcttcaa ctgctgcttt cggtgcttct gccggttcta
    13321 attcttccgg ttttactttt acaccgtctt ttgctgtttc aatgatgtgt gctgcaattt
    13381 catgagcaga ttccattgaa aagcgtgatg cgtaggcttc aatcaacatt ggtaaattca
    13441 aaccgctgac gattgcccat ttatctttgt gctcttcgaa taatgtgttt gattgattga
    13501 acggtgttcc gccccataaa tcaactaaga ataacacttc gtcttgatta tcaaaagaag
    13561 caaccgcttc ttccaatttg gctctcaagt catctggacc ttcacttggt ttcaaaatca
    13621 cggctttgac gttttcttgt tcgccaaaga tcattgaacc ggattgtaag attccttcag
    13681 caaattgtcc atgacttgct aggataatcc ctaccatatt tatacctcct atttactttt
    13741 gttgtaacaa attcaaaaat attaataatt gctaaccatt acaacagaat tatttgacct
    13801 tttccaacaa ccattcttgc tacacctttt tgattttcgc tagtagttcc atcaaaagga
    13861 ttttcctgtc agcaggtact tttcgaatct ctaactccac ttcttgactg ttcaagtaac
    13921 gaaaggaatc aatatcttgt tgatttactg aaacaaaatt ggtaatcatt gatttgcctt
    13981 cagtaaaact cataccgccg atattcaacg acttcaactg tacaccgcag cggacaaccg
    14041 cttcaacatc ttggggattg gtgaataaaa gcatcacttt tgctcgtgat aataatccac
    14101 tatgatagat ttgaatcatt ttctgcacgg ttattacatt tgctgtgatc cccggcggtg
    14161 cagcttcttt tagtagaaac tttctcaatt tatcttgagc gactacatcg ctgatcacaa
    14221 tgatacgttc aacacctgtt tgcttcgacc aaactgttgc gacttgcccg tggattaaac
    14281 gatcatctat acgtgccaat cgaatatcca tctgcaaaac tccttcttcc tcactacttc
    14341 acagaaaccc ctatcatttc tgtgtgaaca accacctcct agcacacact actataagca
    14401 aaaagcgtgc caactttttt acgatacact aattgaattt aaaggattct taaccaatac
    14461 acaaaaacga tacactataa aagtgtatcg tttttgtgta ttcatggcac agtgtattct
    14521 tttttatcta actgattatg gactagacgt agcagataat acatttcttc tatcggcact
    14581 tttactttta tctgatcttc caacagaaca actgaggctg ccaattggct ataaagagga
    14641 tcattcgttg tatttgtcaa ttcgttttct tccacagtca atggttcatt ccgaataata
    14701 cgttcgatca ttcctgctgt gtgcatcact aggttgattt tgaatccata ttcttcatcg
    14761 ccgattcccg tcgtctgaga gatttgtgta gaaaattgcc acattggatc gattaactta
    14821 ctaccattaa taaagatgaa gttatcagag ataaactcaa cacacgtatt tttagctgac
    14881 tcttcatcta gggagatgaa ttcagtttcc tccaattctg attccatcag caactgatcc
    14941 aaaatgactt cgatattttg atcaataaaa cgttctaaag gaataaatgg ggcttggata
    15001 ttcaaatcag taatcccagt agtagctatg atctgatatt ctttttggat aactggcagt
    15061 tcttctttca cttccacaat cgacaaagct agaacttcta attctgtttc ttgacgcttg
    15121 tttaccgctc gttcgatcaa ttctttgatt ctttgtgctg taccttcgcc agatgcgcag
    15181 atagccaaaa cagctttttt cttatgaata ggttttgctt cctgaacaga ttctacagaa
    15241 gatatttctg cgtaacctcg aaaatttttc aatgattcat gcaacatatc tagatctgtc
    15301 cctaatactg agacctttcg tacagtttcc aaaactagag aagttgttac catatcaaca
    15361 gtacgtactg gaattccagt gtctctttga atttgcgaat taaacgacgc aagggacccc
    15421 atatctacta agagtatgac cccgcttcct tcatctactt cttgtacatt gcgttcaatt
    15481 cggtccaatg ctgttttcgg atccatatcc aaaggcatgt ctactgctcg tacgttctct
    15541 gcatctaaca actgttggac aacttggacc atactgcttg ctgtgctgtt tccgtgagct
    15601 gcaacgacta caccgattct gccgcttgct tgatttgccc gtaacgagac gagtagaacg
    15661 gtcaagtaat agtcttcact ttctggaatc tccacttgaa aatacgtttc gatataacta
    15721 cgcacttctt tcgcaacagc atattcatct ggaaaatcaa aagccatctc acggatgtta
    15781 tcattcgtat gccgttcttc tcctaagttg atttttttca aaaaagaact gatatgcaag
    15841 ctcatagcat aaataaagtt ttgctgaaac tcatatggta aagatctttt gaccattaca
    15901 taaatctgat tggtcacttc tatgattttc ggatctacaa attctgctaa tttgctgtct
    15961 gcattaaaag aaaaaccatg gtctttatag aaagacttca gatggatgtt gatatctgta
    16021 gaaatgaaat gattgattgc ttcttgatcc aaaccatccg acttcagtaa agctgcttta
    16081 tctccgatga tatcatatag attataaggt aactcatagg agtccgattg gattttcatg
    16141 atcgtatcat ttggtgctac agttatcttt ggctccaata ttttagaaag ctctgccatt
    16201 gcactgcggt tgcttgctaa ttgaatcaat ccgctacgaa tcccttctgt caaatcatcg
    16261 attgtgatcg agatttcggg tgattgcata tgatttagaa atcctcgtgc acatactaac
    16321 tggatatttg acttcaattg tcctatattt ccataggtca cactgccgat caatgctttt
    16381 gctacatctt ctgtcaaaga aatctttcgc tgcgtgcggt ttgcttcgtg ggcaatcatg
    16441 atttttacta aatcgacttt ctcatttgca ggacgttttt caaaagccgg aagtttgata
    16501 ttgattggga tacgccgaac aaaagtttct aatagagaag aacctgggtc ttctgtggtc
    16561 gcacctacga tccgtacatt tgcttcatgg gatttcgttg tttccccaag acgactgtaa
    16621 gttccatgat ccataaagta aaagatcatt tcttgacctt caggcggcaa ccggtgaatc
    16681 tcatccaaaa acagcatacc gccatccgct tgatcgataa tccccatttt ttcttcatct
    16741 gcgcctgtaa aagcaccttt tgcgtaccca aataaatgac tcatcaaaag ctcagggtta
    16801 ttggcataat cagcacaatt gaaaacaatc agttccgttt cctcatctat tacatgatgc
    16861 gcttttgcaa aatgatacat cgcgtgtgca aaataagtct ttcctgatcc ggtaggtcct
    16921 gtaatcaaac aatttaatcc tcttggcgga tacaaaatcg cagcttttgc ctgttccact
    16981 gaatttttca tgctgccgtt agctccaatg atttttgcaa agatatcctt agtatctatt
    17041 tggagtgtat ttttttcttc agagaccggc gtttctttgt aactgactac atgttttgta
    17101 actggcttta cgtgcgtaac gagtgtttga tatatgtaac gtactggtcg tccgtctgtc
    17161 tttatcagtt ttccttcgcg cactagctga ttcagatctt tactgctatt tgttcgctga
    17221 atccctaaca cttcagcaat ttcctgtgtt gttactcctt gatcatattc tgcctttgtt
    17281 aaatgagcgg ttttttcttt gacatacata taaattcggt caatgcgttt tgccaatggt
    17341 atcgctcctt ttctatagac tattgtactt aggcttcaaa acataatact atgtatttat
    17401 ttctgtttcg tcaactattt acctgatttt gttttgttga taacacgcgg atagatagaa
    17461 atgttcaatt taaaagaacg aaaaatcccg atacgatttt tataatggaa acaagaaaaa
    17521 ga
//
LOCUS       DAYYHX010000044        16922 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000044 DAYYHX010000000
VERSION     DAYYHX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.044"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(270..556)
                     /locus_tag="ACHA6Y_001086"
                     /pseudo
     CDS             complement(270..556)
                     /locus_tag="ACHA6Y_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(733..1911)
                     /locus_tag="ACHA6Y_001087"
                     /pseudo
     CDS             complement(733..1911)
                     /locus_tag="ACHA6Y_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            1941..2351
                     /locus_tag="ACHA6Y_001088"
     CDS             1941..2351
                     /locus_tag="ACHA6Y_001088"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987198.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            2432..3052
                     /locus_tag="ACHA6Y_001089"
     CDS             2432..3052
                     /locus_tag="ACHA6Y_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987199.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            3753..4037
                     /locus_tag="ACHA6Y_001090"
     CDS             3753..4037
                     /locus_tag="ACHA6Y_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987200.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            4040..4957
                     /locus_tag="ACHA6Y_001091"
     CDS             4040..4957
                     /locus_tag="ACHA6Y_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8987201.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            4950..5732
                     /locus_tag="ACHA6Y_001092"
     CDS             4950..5732
                     /locus_tag="ACHA6Y_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8987202.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            5733..6413
                     /locus_tag="ACHA6Y_001093"
     CDS             5733..6413
                     /locus_tag="ACHA6Y_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8987203.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            6406..7302
                     /locus_tag="ACHA6Y_001094"
     CDS             6406..7302
                     /locus_tag="ACHA6Y_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8987204.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            7497..9146
                     /locus_tag="ACHA6Y_001095"
     CDS             7497..9146
                     /locus_tag="ACHA6Y_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8987205.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            9740..10675
                     /locus_tag="ACHA6Y_001096"
     CDS             9740..10675
                     /locus_tag="ACHA6Y_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8987206.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            10668..11438
                     /locus_tag="ACHA6Y_001097"
     CDS             10668..11438
                     /locus_tag="ACHA6Y_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8987207.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            11854..12837
                     /locus_tag="ACHA6Y_001098"
     CDS             11854..12837
                     /locus_tag="ACHA6Y_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM8987208.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            13284..14321
                     /locus_tag="ACHA6Y_001099"
     CDS             13284..14321
                     /locus_tag="ACHA6Y_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8987209.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(14410..14733)
                     /locus_tag="ACHA6Y_001100"
     CDS             complement(14410..14733)
                     /locus_tag="ACHA6Y_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8987210.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(14720..15427)
                     /locus_tag="ACHA6Y_001101"
     CDS             complement(14720..15427)
                     /locus_tag="ACHA6Y_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8987211.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            15513..15854
                     /locus_tag="ACHA6Y_001102"
     CDS             15513..15854
                     /locus_tag="ACHA6Y_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFM8987212.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(16025..16873)
                     /locus_tag="ACHA6Y_001103"
     CDS             complement(16025..16873)
                     /locus_tag="ACHA6Y_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM8987213.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
BASE COUNT         5379 a         2895 c         3537 g         5111 t
ORIGIN      
        1 aatgaaaaac gagaaggaag atgactttaa agtagtttag tatgaaggtc gattgataaa
       61 acgatgtgga ttaatgctaa ataatgagta atttttcaat aatacaaaaa ctgtgtacag
      121 tattagaagt agagaaacgc tagcgtcgaa aaaaagatat ttttcgtgaa aagtaatagg
      181 ggctaaaaag aaagtataca atttatttat gtttctaagc atataaaaaa gagcgttgaa
      241 aggaatattt ccccttcaac gcttcttttt tagaagattt taatgaccat ctcaacttca
      301 ttttcaggtt taggtaattc atggaagctt gtgtttatcg tgcttaattg atcaactaac
      361 catgttttac tttttgactc gtagacaaat ctaaaattaa tatcattgtt ctcgtgttga
      421 agaggtttct ccaaataatc tggtgaatat tcatagtcaa cgtaacctgc taatacaaaa
      481 gaaagcagtc cattatttag tgtcagattt aacggatggt cgtaagcttt tattttttta
      541 atgtcatatt gtacatctta tgaagactta atgactactt gtagtcaata gatttaaagt
      601 atttattttc tcatttatgt atcaagaata aatactgata taataaaaat tatatcaatg
      661 ttttgatgaa gtaatttgga gggagcgtca ataattttgt gtaaataact cgtccttctg
      721 caaaataatg ggttactcag taaacattga agctaatgta tccgtcactt gttggaagcc
      781 tttatggctt ctatttaaga atttttgatt gtatgtatca aagatggata ctaggaaacg
      841 ctctagtgat tcttcatttt gaaactgctc ttttctgcgg ctgtatttct tgatttgttt
      901 cttgaaagat tcgattaaat tagttgagta aatgcttcgg cggataccag gcggaaactc
      961 ataaaaagtc aataaatctt gattttttat cagtgactgc gtcactttag gataagtttt
     1021 ttgctacttc tcaatcatac tccctaaaaa ggtatttgcc tcttcctttg agctagcttg
     1081 ataaacagcc ttaaaatcat cacagatttc ttttcgatct ttgacacgta ctttatgagc
     1141 aatattacgg gaaacgtgaa cacagcaatg ttggtattta gctttaggat aaatctggtg
     1201 aatcgtatct ttcatgcctt ttaagccatc ggtaataaac agtaagacat catgaacccc
     1261 tctggagtaa atgtcctgta acaactcatt ccaaacgtag gtcgattcag tcggagcaat
     1321 cgcataactt agtacttctt tagtgccgtc ttctcgtata ccaatggcaa tatagattgc
     1381 ttctttggag acggtttgcc gttttaaagg aatataagta gcatccatga aaatagcgac
     1441 atacttatca tttagagttc taactttaaa tgcatttact tcttcagtca gcactttagt
     1501 catgttggac atggtttggg gcgtgtagtg atgcccatac attttctcaa tcaagtctgc
     1561 gatttccgac atggtaatac cttttcggaa gaggtgaatg acggtctcct ctaagatatc
     1621 attcgtccgt ctataagcag gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg
     1681 aatctggaga tgaagttccc cgtactcggt cttgaccgta cggtcatagg agccgttacg
     1741 agaattcccc gtgttaaaac caatgcgatc gtacttttcg taatcgagga aagccgttaa
     1801 ctccgttttg agaagcgtat tgacagcttt ttccaaatgg acacggaata attcattcaa
     1861 atcgcctttg ttcgctagag tctttagaat ttctgtagta aaatcgttca tatggaagtc
     1921 ctcttttctg tgaatgtgtt gtggtaactc tattctacag aagggacttc cttttttcta
     1981 tttacacaaa atattttaca ctctcgtcaa atataaaaac tagtacgaaa ttaatttgta
     2041 aattatttaa gcttgataaa ataagcaata tcgatgatgt aaagtccaag atattaacaa
     2101 gaaaagtaag cgaaccagta aagctaacgg cacaagataa aaaaaatgga attgttcttt
     2161 atggtgatga tgcaaagtat aactataatt gggatacctc ctcagaagct gaggtattaa
     2221 gtattcctac taccgatact tcaggggaaa aagtcacctt taaaataaat tttataacgg
     2281 caatttctaa gttggagtat gggaagcaaa aaaagagaga taacaatgat caatatctac
     2341 ctaaaaaata acgaattaat gatgcatatg ttaaagttgt ttttttgaat taaacaactc
     2401 aatttattca gttatatgtt catcgcagca agtgaacatt aatcgagtta taaaattaat
     2461 aggagctaaa aatataaata aagttcgatc agaatttatt tttccagaag atttttttaa
     2521 ctggtttttt tatcaattca gtgaatgcaa gcgactctta ggtgagaatt attacttaaa
     2581 aggaatcggc ggatttatgg ggaatgtggc tgatgaacaa aatattataa aaggaatatc
     2641 tgaacaaaca tcagagctta cagtaactaa agcatttata agtaatggtg aaagtttgaa
     2701 aaatgtaact gcgcgaatta gaaaagaaaa tgaagtagac attgtttttg gaattgacca
     2761 tgaatgtaat tcagtgatat atgtaacaca atcagcaaat ttaattcttt ttgagaatgg
     2821 ttgcaaggac gattttatgg tggtctattt gtacgatata ctaattccaa aacttcgttc
     2881 cttatatatg tcttctgaaa gtatttttgt gagtgaacaa aagggatctt tctcgaaaaa
     2941 aataggtatt gaagtaattg aatcgattat gtgtcataat catattacgc tagaagaatt
     3001 agaaattttg atagggcaaa aaaataataa cacgaaaata caagtttctt agaaatcatt
     3061 ttttattgag atatctagat aaatgattta ttcttcaatc aaccaactcg ccaagctgag
     3121 gtaatgtttt gaggtaatga taacatacaa ctttttatac cctagtaaaa ctctataaac
     3181 aaagacttat taatttcgca tgaaatacat tcctgtaaaa tgcaataaaa tagagttttg
     3241 tttaaccagg tcttaagaaa gcccgtaaag cttcacagtt ctcaaaacgt taattgcgat
     3301 tatcgttcga gttcacgagt tacgagaatc gtactgggcg aagcaatgct gagcgcagtt
     3361 accttaatcc taacttttct ggcactttcc acaatttgga aagtgccttt tttgtgtttt
     3421 tagctatgtt aataggtagg attcttggtc tataatatca ttcgatctat aaacaaacgg
     3481 gaaaaagata aagtatcatc aaacaatatt atttatccgc ttctgaggtt gtgacagaag
     3541 tggtcagctt caagaaataa gaaggaattt acaaaaattg cttctcatgt ttgataaata
     3601 ttgtgacttg tgaccgagga gttggcagga gaacaccgtt tattcggaag aagggattgt
     3661 gaaaagtctt ttgtcataac ctcttatttg gtagctaatt gtaaatccca aacggcatgt
     3721 attatttttc ataagtccgt ttttaaggat taatgttttc ttttgctttt tatatggtct
     3781 ggcttcagcg agaagttcca acgcatatac aaggacgtct gatgtcaacg ataacaacga
     3841 ttaccatggt ggccagtcca cttggattag cactgtttgg tgcgctcttt gattggcaga
     3901 caactcaacc acgtctgcga gacagtctga tttttcttga aattcaattg gaaaatttac
     3961 actttcttta tacatttctg gctgctttct ttatacccgt caactatact tcacttatca
     4021 ataagaaaga aggataagta tggattttgg aaatgaaacg gtcttgaaag caactggtat
     4081 aaccaaaaaa tacggtgctg ccaaagcatt agataaagta tcgatcgaaa tcaaacgtgg
     4141 aatgatctat ggtttgattg gggagaatgg agcgggtaag tctaccttta tgagaactat
     4201 tatgggtttg atctcgattg atgaaggttc gatcgaatta tttggaacga cggatttgca
     4261 ggcagcaaga cgacgtatgg gtcaatcgat tgaaacccct gctctttatc cagaattaac
     4321 tgcaagagat aacctacgta tccaagcagc gaacggcggt gtgtcggata gagaaatcga
     4381 agacctgctg aaaatgatgc gattggaaaa tacagggaaa aagaaagcaa agaatttctc
     4441 tttaggtatg cgccagcgtt tagctattgc aaatgcactg atcacgaatc cagaattttt
     4501 gattttggat gaaccgacga atgggatgga tccagcaggg atggcagaaa tgcgagaaat
     4561 catccaacgt ttggtaaaag aacggggcat caccgtcttg ctttctagcc acttgctaga
     4621 tgaattgtca cagatcgcca ctcactacgg tattttgcat gaaggacact tgatcaaaga
     4681 attatcgaaa gaagaattgg cacaagaatc tcgtcaattt ataaaaattg acacttcggc
     4741 gacggaacaa gcagtaacgg ttctcgactc tcttggttat agggattatt ttgtccaatc
     4801 tagccgtgtt atccagcttt ttgaaggaat tgaccaagta gcagccatca atcaagcatt
     4861 ggtagaggca aaagttcctg tggatggtat ccacctagtt gggcaaaaac tggaagatta
     4921 tttcttacaa ttaactggag gcaatccaca tgtttaatct attaactgct gaaaaaataa
     4981 aagtatggaa gagccgtaaa atgtgggtct cactaggact gatggtcgta cttccgcttt
     5041 tttattcttg gaacgaatgg tatatgcata caaaatacgg caatgaatta aaccaagcaa
     5101 cggatacagt catcaacggt gcaacaggga ttttaatggt ggaaaaaatg gctgcatgga
     5161 ttcttctggc tttcgcagca tttgcgtgct tttatattgg ggaagaattt caaaacggta
     5221 cgattcgcaa tacgttatct ttaggacgga atcgaatgac ttattatgtg tcgaaacttc
     5281 tcgttacgct tttgatcaca acacttggcg tggttttgat tacggggcta gccatgattg
     5341 cttacacact tgctttcgga tttggtgaag ttgagggaat caaaaactat ggaaattatg
     5401 ttctaaaagt atttcctgta ttgcttctgc tgatattagc gacattatct gttccagtgg
     5461 ctttgacatt catcactaga agtaccagtg tcagtttgct tctttctttt ctttatatca
     5521 tgggaacggc cttcgttccc ggtgttttcg caaaaatcaa aggcttggaa tttctaacgg
     5581 agtggtttac ggaaacgtgg ctcatgtata cggactttgc gcaacaagca acttattcgc
     5641 aagcaccgaa aatgattctg gtcagtctag taacaatcgt ggtatcatca gctctaggaa
     5701 tgtttatttt ccaaaaatct gacattaagt aggtgttcct gctggaaact attttgatta
     5761 tcgaagacga tgaagctgtt cattctttac tggaggaggt tcttgaacag agatacaaac
     5821 ttcttgatgc ttattcaggt acggaaggaa aattattgtt aagcacatat cctgtcgatt
     5881 tgatccttct agatctcatg cttccagggc tttcaggtga agcattgtta gcagaaatcc
     5941 gacagacatc gaatgttccg atcatcgttc tttcagcaaa aagcgatcaa agagataaag
     6001 tttccttgtt ggcagcagga gcggatgact atgtgacgaa accatttgat atcgaagaac
     6061 tgttgttacg aatcggcatc caacttcgtc atcagacttc tgtccaagta aaagatctgt
     6121 cgcaacgcat ctattacaag gaaatcttac tggacaaaga gtcgagggat gtaaatgtga
     6181 atggtgtggc tgtccatttg acaggaagag aattcgatct gctgaagctt tttttagaac
     6241 atccgaaaaa agtattttcg cgagcaaatc tttatgaaac agtttggcaa gaaccatatt
     6301 ttgatagtga gaaaacagtg aatatgcata tttccaatct tcgagggaaa ctagcaaagg
     6361 cacatgctgt gtatattcat acggtgtggg gagtggggtt tagatttgat taagtggctg
     6421 ttgcttctcg ttgttatctt tatgggaagt tttattttct tcctgcaaag ggaaatcaag
     6481 agattaacta agaaaatcgc cactctttct gaacgacaag aatatggtgg tcgtctttca
     6541 gtcgaatttc gagaaaagaa tctaatggac ctagtagatg cactgaatac atgggtggat
     6601 gaaaatgaac aaaaaggaca agcatttcaa gaaatggaag aaaacatcag actatcgatc
     6661 gtcggccttt ctcatgatct cagaactcct ttgactgcta ttaacggtta tgtacaatta
     6721 ctggaacagg tagaggatac ggagaaacgg gagaagtatt taacgatcat caaacaatct
     6781 gttgcaagat tactcagtat gacggatcag ttttatgatc ttgctcgaat agagacgaag
     6841 caaaaagaaa tggatctcca tacgctttct ttttctaaag aggtggaaga agtatttttc
     6901 tctttttatg aacaattttc tgagaaaaat atcgaggtag cgttcgctga acactcgata
     6961 aatacaactg tattagcaga tccgctcatg ttgctgcgag tgctgcaaaa tatcatacag
     7021 aatttattgc gttatgcaga tggacaagca aaggtgacat tttcaaaaga ggattcattc
     7081 attcgattaa cagtggccaa tcctatcaaa ccaggagcgc aaacctctgt tgaaaaagtc
     7141 ttccagcgtt tttataccga aaataccagc cgcacaaata cggaatctag cggaatcggg
     7201 ctttacctgt caaaaaaatt ggtggaagga atgggaggag agatgaccgc tagattagac
     7261 gggggcatct tctccatctc cgtcaagttg cggagggttt aattggctct aatttacctg
     7321 aagtgaatga caaaaaattg tatctcgttt taaagacatc ttcttttttg gaaggtgttt
     7381 ttattttggt gtgattgata cgaattaggt aacgaagaca gttatttttg tacccccttt
     7441 tcaaatagcg caacagtgct atactaaaac taacaaatca aggaacggag agggatatgg
     7501 gccaaagaga atttatcgtt ttaattatta ttgcagttgt tattttatta gttgttttag
     7561 acattttttc acgtttgaaa gtaaaggaaa cagttcgtag taattgggga aaaattcctt
     7621 atcaaccgag atttgataaa gaagaaagtt tgaaggatgc gtggctcacg gagaaaaaat
     7681 tccgttcatg ggatagtgaa atcgacgatc ttacttggta tgacttggat atgttcgaag
     7741 tatttgaagg gatcaacagt acctattcta gtgtaggttc agaagcgttg tatcagcgtt
     7801 tacgtagctt tgattttgga gaagattatc agttagaaaa actaattgct ttttatcagg
     7861 aaaatcctca actgagagaa agaatccaat atcaattcgc tcgtttagga aaaaaagatc
     7921 ataattttgc aaaacaatat ctagcggatg gaaaaagtaa aaagattgga cgcgtaggtt
     7981 tatttatttt tcttggcttg cttccgctgg tgggtgtagg tttgcttctt ttagggcaag
     8041 tactaggaat ctatctagca atcggtggct tagtattcaa tacggtctat tatctagtga
     8101 agaaacaaac actagaaaca gagctgaata gcatgagtta tttggtttca acgatttcag
     8161 ccgccaatca gttagcaaaa atgacgacac cacttcaaaa agagctgaaa cagcaaacta
     8221 aaccattaag agacatccct aaattaggtt tttcatttcg aatgaagagc gggagtgaag
     8281 cagaattgat ttttgaatat gtcaacatca tgttcatgct gccatttatc tcctatcatt
     8341 tcgttctcac taaattagaa aagcattcaa ctgaagccat caagctgtgg gaattgctgg
     8401 gagaattgga agttgcagca gccgttttga attatcgcac ctatatgccg atcacttgcc
     8461 gaccagagtt tactgagggc ggtgtgacag cgaaagatat ctatcatcca ttgttaaaag
     8521 aagcggtagt taatccagtc aactggcaga agaatacatt agtcacgggt tccaatgcat
     8581 caggaaaatc tacgtatgtc aaaagtatag ccatcagctg tattctagca cagacgatcc
     8641 aaacagcgat tgcagaacag tttaccctgc aacctggaca tgtgttgact tccatggcaa
     8701 tcgaagatga tttatttgaa ggcgacagtt attttgtttc agaaatcaaa tcgatcaaac
     8761 gtttgctgga ccaagtggct tccaaagaac gctgctactg cttcgtagat gagattttaa
     8821 aaggaacaaa tacgatcgaa cggattgctt cttccgcttc agtcgttcgg tggcttgcgg
     8881 agtatccatc tttagcattt gtcgccacac atgatatcga attgacagag attttaaaga
     8941 attattgtga gaatgtccac tttgaagaac aagtaacaga aggaaaagga atcagttttg
     9001 atttcaaatt aaaagaagga ccttcaagga cccgaaatgc gattgccttg ctgaaagttt
     9061 tgaattatcc taaatccata gttgaacagg cccaaaaaga aagtttgttg ttcgatgaac
     9121 agcgtcagtg gtatccgttt gattaacttt atcaaacgaa gcatgatgcg tgggtccaaa
     9181 aagacgatta tcaaaagaag tacattgatg ccaaaaaagt ggcagaattc aatgggaacg
     9241 agtctgctgt ccagatgatc ttggaagatt tagcaaaaga cgatacaaaa taaaccacgc
     9301 tgtcttcctt ctagcagctt ttatcgaaaa aacttagcat cagcgacgag acgcacgttc
     9361 catggcaaag ttctccgtaa aacaaacagc gaaaacagaa attgaaaata actatttcca
     9421 ttttatttat ttcggttaaa ataaaagaaa aaagctttcg ataaaaggac ggaggaaagc
     9481 ttaacatgaa taaaaaagac aagaacctca gttataagga tcttctatat gtcatcgtag
     9541 tagttgctgt cggtactttt atgattttaa aagttccaga gttgtttaga tagttattct
     9601 atcgaagatg atcatgaact agaaaacaca tctcctcttt gttagcaggt gtgtttttgt
     9661 gtggaagcca agttgttttt tcatctgctg tattccatga tacaattaag aatcatgtct
     9721 ataaggagat aggaaataaa tggcaaatgc gcttgaaata aataatttga aaaagatcta
     9781 tggaagtggg gtcgaagcat tgcggggaat cgatctttcg gtgaaagaag gagatttcta
     9841 tgcgctatta gggccaaatg gagctgggaa gtctacaacg atcgggatta ttacttcttt
     9901 agtcaataaa acatctggaa aagtgaatgt cttcggttat gatctggaca aggatctagt
     9961 tcgagcaaag caacagattg gattggttcc acaagagttc aattttaacc cttttgaaac
    10021 ggtccagcaa atcgtcgtga atcaagcagg ctactacggt gtctctcgca aagaagcgat
    10081 gaaacgcagc gaaaaatatt tgaagcagtc caatctttgg gaaaagagaa atgtccgtgc
    10141 acgtatgctt tccgggggaa tgaagcgaag actgatgatt gctagggcat tgatgcatga
    10201 accacgtttg ctgatcttgg atgaaccaac agcaggagtt gacatcgagt tgagacgcga
    10261 gatgtgggag tttctacgag aattgaacga aaatggtaca acgatcattt tgacgacgca
    10321 ttatctagaa gaagctgaaa tgctttgtcg caacatcgga atcatccaat cgggtgaact
    10381 gatcgagaat acaagtatga aagagcttct gtcaaaactg caatttgaaa catttatctt
    10441 tgatttagcg ccatatgata ccaaacctgt gattgaagga tatgcttgta catttgaaga
    10501 tgagcagaca atcgctgtcg aagttgaacg aaatcaaggg gtgaacggaa tcttcgatca
    10561 gcttacaaga caaggaatca aagttctttc tatgaggaat aagtcgaatc gtttagaaga
    10621 actgttcttg aaaatcacag aagaaaataa tcatacggag gagaaaaatg tttaatcttt
    10681 attttactgc attaaaaagt ttagctatca aggaaacgaa ccgctatttg cgtatatggg
    10741 tccaaacact ggttccgcca gtgatcacga catccttgta ttttgttatt ttcggaaaaa
    10801 tgatcggtgg gcggatcggt gacatgggtg gtttttcata tatggaattc atcgttcctg
    10861 gtttgattat gatgtctgct atcactagtt cgtatgccaa tgtttcttcg tccttcttct
    10921 ctcaaaaatt tcagaaaaat atcgaagaaa tcttggtagc acctgttcct acgcatgtga
    10981 ttatttgggg ctttgtgatt ggtggtgtgg gtcgcagtat tctcgtcggc tctctcgtaa
    11041 cgatcatttc cttgttcttt gtaccacttc atgtgtattc atggttcatg gtcatcatta
    11101 cattattgat gacggcaatc ttgttttcat tagcaggact gctcaacggg atttttgctc
    11161 agtcttatga tgatgtatcg atcgtgccta catttgtctt gcagccgtta acttatctag
    11221 gtggcgtttt ctatgccatc tctatgttac cgcccttttg gcaagctgtt tcaaaagtca
    11281 atccgatcgt ttatatgatc tccggtttcc gttacggatt ccttggaacg atcgatgtgc
    11341 cagtagcaac atcgatgatc gtacttgtgt tattcatcgt tgttttgtac gccgttactt
    11401 ggtatttgat caataaagga agaggattga gaagttagaa aagaggttgt gaaatcagcg
    11461 ttttgacctg agtattagag aaacaaacgg gaaaatagtt cctcatattt tgttgttttt
    11521 tgagcgaata ccgagggtca gctgattgaa caccgtttag tggagaggtt gtgaaaaagc
    11581 tgttctgcat caagtaataa gaacagccaa acaaaaatag ctcctcctat ttttcgtttg
    11641 gttggactta ttaccgcaga tgcaagcttt tgaacaccat ttattccgga aaatggactg
    11701 tgataagatt tttgtcacag tctttttttt gctcaatcag aaacatcttt taagctgttc
    11761 actttgcttc tttttttgtt tcctagtata atgacaatgt ttttgagaaa gattgcgact
    11821 tcactgcttg atagaatgga gagtaacaaa taaatgacta ctaatttttg gaaagagctg
    11881 ccaaagcctt tttttgtttt agcaccaatg gaagatgtaa cagatgttgt atttcgccat
    11941 gtcgtaaagc atgcagcagc accggatgtc ttctttactg agtttacaaa ctcagacagt
    12001 ttctgtcatc cagatggaaa agacagtgta cgaggaagat taacatttac agaagatgaa
    12061 cagccgatgg ttgcccatat ttggggagac aagccagaat tttttagaga aatgagcatt
    12121 gctatggcag aaatgggctt ccaaggaatc gatatcaaca tgggctgccc agtccctaat
    12181 gtagcagaac gtggaaaagg cagtggcttg atccttcgtc cagaagtagc ggcacaattg
    12241 attgaagcag ctaaagctgg cggtttgcct gtcagcgtca agacgcgtat cggctatacg
    12301 gaaatgtctg agatggaaga atggatcagt cacctacttc atcaagatat agccaatctt
    12361 tctgtccatt tgcgtacgcg taaagagatg agtaaagtag atgcacattg ggaagtcatt
    12421 cctaaaatca ttgctttacg tgaccaaatc gctccacaaa cactaatcac gatcaatgga
    12481 gatatccccg atcgccagac aggagaagaa ttggccgaga aatatggcgt tgatggcatc
    12541 atgattggcc gagggatttt caagaatcct tatgcatttg aaaaagaccc gagggaacat
    12601 acagaaaaag aactgatcgg actacttcgt ctgcaactag atctgcaaga tcattattca
    12661 gaaatcatcc cgcgttccat cacaggacta catcgtttct ttaaaatcta tatcaaagga
    12721 tttccaggag caaatgactt gcgagttcag ttgatgaata cgaaatctac tgatgaagta
    12781 cgagagatct tagagacctt tgaaaaagaa catccaacgc ttttcagtga acaatgattt
    12841 cttgcttgaa caccgtgaat tagaggtcgt gaaaaagctg tcctgtatca agtaataaga
    12901 acagccaaac aaaaatagct cttcatattt tgtcgttttt tgagctaata ccgagggtca
    12961 gttgattgaa caccgtttat atcagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    13021 ggagcaattt caaaaaacag cttctcatgt ttgatgaata ttttgacttg tgaccgaaga
    13081 gttggtagga gaacaccgtt tataagaggt tgtgacaaaa gtcttgtcgc aacctctttt
    13141 ttaaccaaaa aagtgcaaat agtttacttt aagtaagaga ataattatga tgaataaaaa
    13201 aggtggaaca aatagcatac tatatatatt gaaatgaaca ctgtatggat gctccaactt
    13261 gatgaagcta aggaggaatt cgtatgtcag aaaaggaatt aatgaaggta gtcggttttt
    13321 atgaagggtt gccgatcgat gatccaaaaa gctttattga tgaaaaagaa cagatacctg
    13381 tacccagttc acgggatctt ctggtaaaag tcaacgcagt atctgttaat cctgtcgata
    13441 cgaaacttcg ccaagataac gggataagaa atgctttacg tatcttaggt tttgatggag
    13501 tcgggaaagt tgttgctgta ggtgacgaag tgcaaaaatt ttctgtcggc gatcgcgttt
    13561 tttacgcagg tacaacaaca agagcaggaa gcaatcaaga ataccaacta gttgacgaac
    13621 gtattgtcgc tcttgcacca aaaaatcttt cagatgaaga agctgcagcc ttgccgttga
    13681 cctcattgac cgcttatgaa ttgttatttg aaaaatttgg tctaactcca gaagaaaatg
    13741 ccaatcgcgg gaaaaaaatc ttagtgatca acggatccgg gggcgtcggt tcgattttga
    13801 atcaactagc gcattgggct ggcctagaag tctacgcgac tgcaagtccg aagaattttg
    13861 aatggctgaa aaaaacaggt gtagactatc caatcgatta tcatcacgac ttaagtaaaa
    13921 gttttcgtga attaggaatc gatcaagtag agtatgtcgc tgtattattc gatattaccc
    13981 gctactttga tcaaatcaag caccttgtcc gaccatttgg acatgtggga acgatcgtag
    14041 ggatcaaaga gccgttagat ataggtgttt ggaaaaacca atctatcagc ttcgactggg
    14101 agtatatgtt cgctaaaaca gactataact atgaaatcga aacgcaagga gaagctttag
    14161 cgcacattgc tgctttagca aatgaaggga ttatccgcag tacattgagc aaggtctatt
    14221 cagatgggat gaatgcgaag aacttgaaac aagcaaccaa agacgtagaa acaggacata
    14281 tgttaggaaa agtcgttgtt agtggtccgt tcaacggata agcaagaata cgccgatttt
    14341 tttcttattt gactttgata aaaacaagag cgcatgacag taatttgcca taagctcttg
    14401 tttttttatt tataataagc gaatcaacga aagggaaaga attcctgcca agacgatcac
    14461 caataagctt tttgaaagaa tagcagctaa aagtgtagga actgaagcca aagcattttc
    14521 gagattgatc tgcggcagat gtccgatatt ttgggtaaat agactgctga accataaagc
    14581 agacataatg acgatcggga caaaacttaa atactcaagc aacggttgcg gtaaatcgaa
    14641 ttttttcagt aagacaaatg gtaggaccct agaaagccaa gttgctaaag tacaacctaa
    14701 aatagtcagc aatgtaaact caaaagaagg catgtttgat ccacaccccc aatccgcacc
    14761 caatcagtgt tacaactaca atcactaagt tgcttgggat aaaaatcagt ccgacatata
    14821 ccagaatcaa agtgagacca atcaggatca actgtaatcg tttagaggtg tttctatcag
    14881 aaatgacttg aagataaagt aacccgataa acatagcgac gatggcgaaa tccaaaccaa
    14941 actttttcgg atcagtaata aaattcccta aaagtgcgcc aacaagagaa gaacccaccc
    15001 acgtcaaata agaaatccaa ttagctgtat tcatccaacg aaagtttaac ttttgtccag
    15061 tatagttcaa tttattcatt cccagtgcga aactttcatc tgtcagcaat gtgccaatca
    15121 gcagattttg aagcagccga tttttcttga aataaggagc aatcgtcata ctcatcagaa
    15181 tcattcggga attgactaga aaggtcgaga aaacgatgga aagcaatggg ctgtgactag
    15241 caagcatgct gactgtgata aattgggcag aaccagcata gaccagcagg gacatcaacg
    15301 tgacgactag tggatgaaat ccggcagctt tcccaacgat accaaatgca agtccaatac
    15361 cgatataacc gaaaacggta ggtaacgtgt ctttgatcgc agtttttata tctaactttt
    15421 catccaataa caaaactcct ttagcacgac ataaattttc ataattgtcg ttaaacttgt
    15481 gaatattcta aatttttaat gagcatttgt caatggtggg attgaaacgt aaagaactag
    15541 cgggtcgcag atttacacgt attgtttcgg tgggaggagg cgcgaaaaat cttgcatggc
    15601 tacaaatcca agctgatatt ttcgatgcaa cggtctattg tttaagtgtg gaggaaggac
    15661 ctgcattagg cgcaacaatg attgctgcac tagggttagg gtggttcgac acacctgaaa
    15721 gttgttgtca gaattttatc caatatacag atgcagtgga acctctctta caaaatgtag
    15781 aaaagtatca taaaatctat caacaatgcc ggaaaatcta tccagcaacg aaggaaattt
    15841 gtcatgactt ataaaacaaa acgcgtacct aatagtcaaa tgactaatat tgggtacgcg
    15901 ttaagtcttc tttacttcat cctttgcatg attcgtgttt ttaaagcaag gtctgctgta
    15961 tttcttgtgg atttaggatg gttgttatcg tttacttgct atttttcatt gaaatcgtct
    16021 ttctttaccg tttctttagt tttttatcta tttttttctt ttggttagaa aattcttttt
    16081 cccgaaagtc ttctttcagg atttttatag agtagcgttt tgaataagag gcgtcagtat
    16141 gatcgacttg gcagacgaac caagagaaca tttgcctttt ttctgcatga ggtgcttctg
    16201 ctaataattc tttccataaa taataacagt cctctatcag aagaagggtt cgttcatcct
    16261 caggctccca atagaatgtt tcgagagtag caatcacttc attgatcaat tcgaatgcgg
    16321 gaagataggc attttgctga ataagcgatt caactgcttc ttggatgaac tttcgcatct
    16381 ccatgatgaa cttcgctgtt ttcctgtttt tgtttttctt gataatatga tcgtatttat
    16441 ggatgatagt tgttaattta tctgaataat ttattgcatg aaatggcatc ttaactttga
    16501 agcgttcgat aaagtgcgga tgttctataa gaatctctgt taaaagctcc cgtacaagag
    16561 aggaactagc ggaggaaaca agggacgaga gttcggaaag tgagtagttg gattttttta
    16621 ttggacgttc ttcgtaagcg aataagacag cagccatgtg tttgcatttt tttccttcta
    16681 tggcatatag acaatcacaa gtcatgttgg tcttttcatc ccaagtgatc actaccttat
    16741 aatcttccgt gccagaaact gttgcggaca agtacttgct tgtatagttc aaatgacgga
    16801 caaaaccgtg cagatgatag ctgtaaccgc gttccaaaac gttttcagga aaatactcaa
    16861 gccaattatt catctagttc tcctttcatg aaacacttat ttatatttag tataaagaaa
    16921 cg
//
LOCUS       DAYYHX010000045        16578 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000045 DAYYHX010000000
VERSION     DAYYHX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.045"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..228)
                     /locus_tag="ACHA6Y_001104"
     CDS             complement(<1..228)
                     /locus_tag="ACHA6Y_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987214.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNE"
     gene            complement(235..783)
                     /locus_tag="ACHA6Y_001105"
     CDS             complement(235..783)
                     /locus_tag="ACHA6Y_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM8987215.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(828..1160)
                     /locus_tag="ACHA6Y_001106"
     CDS             complement(828..1160)
                     /locus_tag="ACHA6Y_001106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987216.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1174..1431)
                     /locus_tag="ACHA6Y_001107"
     CDS             complement(1174..1431)
                     /locus_tag="ACHA6Y_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987217.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1626..2168)
                     /locus_tag="ACHA6Y_001108"
     CDS             complement(1626..2168)
                     /locus_tag="ACHA6Y_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFM8987218.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(2189..3280)
                     /locus_tag="ACHA6Y_001109"
     CDS             complement(2189..3280)
                     /locus_tag="ACHA6Y_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8987219.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3288..5498)
                     /locus_tag="ACHA6Y_001110"
     CDS             complement(3288..5498)
                     /locus_tag="ACHA6Y_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8987220.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5514..8042)
                     /locus_tag="ACHA6Y_001111"
     CDS             complement(5514..8042)
                     /locus_tag="ACHA6Y_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8987221.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8094..8504)
                     /locus_tag="ACHA6Y_001112"
     CDS             complement(8094..8504)
                     /locus_tag="ACHA6Y_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM8987222.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8517..8735)
                     /locus_tag="ACHA6Y_001113"
     CDS             complement(8517..8735)
                     /locus_tag="ACHA6Y_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFM8987223.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8732..9724)
                     /locus_tag="ACHA6Y_001114"
     CDS             complement(8732..9724)
                     /locus_tag="ACHA6Y_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8987224.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(9803..10714)
                     /locus_tag="ACHA6Y_001115"
     CDS             complement(9803..10714)
                     /locus_tag="ACHA6Y_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8987225.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10733..11077)
                     /locus_tag="ACHA6Y_001116"
     CDS             complement(10733..11077)
                     /locus_tag="ACHA6Y_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987226.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11097..11375)
                     /locus_tag="ACHA6Y_001117"
     CDS             complement(11097..11375)
                     /locus_tag="ACHA6Y_001117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987227.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(11734..12234)
                     /locus_tag="ACHA6Y_001118"
     CDS             complement(11734..12234)
                     /locus_tag="ACHA6Y_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987228.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12235..12780)
                     /locus_tag="ACHA6Y_001119"
     CDS             complement(12235..12780)
                     /locus_tag="ACHA6Y_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987229.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGAIV"
     gene            complement(12777..14033)
                     /locus_tag="ACHA6Y_001120"
     CDS             complement(12777..14033)
                     /locus_tag="ACHA6Y_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8987230.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(14394..14705)
                     /locus_tag="ACHA6Y_001121"
     CDS             complement(14394..14705)
                     /locus_tag="ACHA6Y_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987231.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(14728..16203)
                     /locus_tag="ACHA6Y_001122"
     CDS             complement(14728..16203)
                     /locus_tag="ACHA6Y_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFM8987232.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
BASE COUNT         5051 a         3572 c         2365 g         5590 t
ORIGIN      
        1 ttcattttta cttgttattt tttcattgac tataactagt tcatctggta aaaaaattaa
       61 ccaacaaaca tcgacaaaca gtaaatgata taatccattt ccacctccaa gcatattcaa
      121 tactggtgaa catgccttag aaacacgaat atagttatgg tttaaataga tattttcttt
      181 aaacattgct tgtttaaaag ccatatgctc ttgttctaaa cttttcataa acttttactc
      241 taatgcacag ctagcagcag ctgctgcaat tcctgctaca atatttacct tagctaactc
      301 tttagctgca gcttttccta attgtttttc tgcaattttt aataataaat ccgcagcttt
      361 ttcaaaatta aaagctttta atgcttcaat aaaatctccc tgcgtaccta tagcccaagc
      421 cactcctcct ccaggaagag aattaatggc tacacatcca gcatatcgtt tccaatcaac
      481 tttctccatt ctaggaacaa taatatgttc ataaaagact cttgctgctc ttccttgttc
      541 gttatcttga ctgattcgtt cacgaatagc accaaaatcc tttatatggt atacaccatg
      601 ttcgtctaaa aaaccaactg tatcaaaata aaaagttaat tcatctacta atttattctt
      661 tatttctaaa tcaactgtat tcactttttc tgtggcatac acacttgttc cagtactaac
      721 aaaagtactt aaacctaata agaaaacacc taaaacacca aatattttaa ttattctttt
      781 cataatttct cctccataaa aaataagtta ctttttaaat atcttgttta tgaattcatt
      841 ccccaaaggc cttttaagat tgatacagca gtccgttcac ttcttttaga cttgtcatac
      901 aagtctttta aatgatccca tatcagacta gtttgtctat ctagcttcaa tccattaaac
      961 gctaatgaac ggttcatttt ttctattact ttagataaag ctacagaaat ttttatacct
     1021 tcacaacgcg catcttggat ttcaataata gtatcatcaa ttagtttttg aatttcttgc
     1081 ctatcaacat attcaatctt agaaagagaa tcttttaaag ctactaatga ttcaacgatt
     1141 tcttttttct cttcagacat tttcttcctc cttctagtga cccgtaaagc catgtagagc
     1201 agagtccatt gaaccagtaa ttacactatc tacaacgcca ttccaattta ctctacattt
     1261 tccatttttc agatttgtcg gtccatacgg atagcctttt tgacattcaa tgttcggatt
     1321 ataccagata ggattatacc attcagccgc agaagcagat tcagtcgata caaacaatcc
     1381 cacaccaaca aatccaatac acatcacact atagccaatt agttttttca tctcgtatcc
     1441 atctcctaaa aaatagaata gttcttatag ttattcataa gacatatttt ttccgttttt
     1501 gttttatcct ttctgtccta taacaacttt tttcaagtaa agaatgacat agtttttctt
     1561 aaaaaaacag tctgtttttt tctatttctt cggtgtataa taaatatgag tgttcatttt
     1621 ttattttaat acgcactccg tacataataa atcttgatcg catcaatctt ttgagcttct
     1681 tgatctactt tcaattgaag taatacaaat tgagtaacct tattcattgt ttcttctcca
     1741 aataacaaat attgatccgg attctctata tcttgtgtca cctggtaaag tttcccagtt
     1801 gttttcattt gtacatgcgg gtctacatca atactattgt ctttaataca acgatctgtc
     1861 atatagtccc tcacagcttg attgcgttta taaacatctg tgaaattact ccattttcta
     1921 gcaaaatctt ttaagaaatt ttttgtcatt tcatctattt gcatttgttg cgctgtctcg
     1981 tcttctaatg aaccaatttg actgttactt tcagtacttt gaagagttcc acttgtttct
     2041 tttactgctt cgattgtttc ttctgtcgtt ctcaattgtg ttttttgttt ttcagaagaa
     2101 aaccaatgac aaccagtaag acctatcaaa cataaccaac taaatactaa aattatccct
     2161 tttttcacgt ttctagtcac tgcctttctt atttcacctt acgaaccaga ctacgaattt
     2221 ttgtttgatt gtacgtgcgt tgataccgag ccataatccg tccttgctcg gcattctgtt
     2281 ccacactaga gaaatgttgt ccgccatatg aaacatctgt tataattccg gtgtgaccat
     2341 agattccagg agacaatgca ccacctgctt gccaattcac cacatctcct gcttgtaaat
     2401 cacttggttt tgggttcatg attacttgcc aaccataggt tgtccagtta taatcttccc
     2461 ctattaacat cgcaaacatg tgtccagaac ccattaactg tgggccacct aattgagata
     2521 cataccatgc cgtaccacca taacattccc cattatttac ggatcgtcca atgatccgat
     2581 ccaaaacctt cgcattgcct ttactttttc ctgcaataag cgaagttttt tttgttggta
     2641 ggctattcca atgcttgatc gcaaaagtat catattccgt taagttatag gacgttatga
     2701 tactcattgc tcctcgataa tacggactcc cttcggtaat acctgctgcc gcatagcctc
     2761 cacgacctag aatcaataaa caacttccag gatctttatt gcccacacat tgattaaagt
     2821 tgctttggct cgccaccata aagccataat agtcataaca agcttttaag ttcggaaact
     2881 tcatatagaa accaccttca gaaccaccaa ttccacgtgc agaaccttgt gggtagggac
     2941 aaccaggaaa ccaggtaatc ccaaaaaagt tcgtatcgtt tcttccagat tgtgactgtc
     3001 cataagctga ttcatagcct aattgagaaa gaatcacact cggtaataac tgatacttta
     3061 cagctgcacc aactgctgct tgcgcatgtt catctgtgat tggaccacgt cctttaatct
     3121 ctgaaaaaat cggtaatcct tcggtatatt ccccattaaa agcgacagct agacctgtag
     3181 cttctcctcc atgctcagaa agttctcctg aaaaaaagaa tggcaataga aaaaagggaa
     3241 gaataaagaa aagaagacaa cctagacacc ctttcttcat ttcttcctca tcctttcttc
     3301 gataatggtt tttggactgt tcgtcctttt ggtaaagtaa aatcagttcc tggttttgcg
     3361 tccaaaatcg gcagtgtttt aggatccttt tctatttttt ccgattgctc tgttggctta
     3421 tctaaagaag aaaatgcagg ttgtaacgat tttggtgcgg aaatcgacgg tttactttca
     3481 gacgaacgtt gccccggttg ttgcacagaa cgatttcctg atagagtggc taccgacgaa
     3541 ttaggaacag agtattggtt caataactgc tgcattgctg gataactccc atttttcgcc
     3601 tgatttttca cttggcgaat ctgttgtgaa agagaaccag cagctgttaa ttgtggcacg
     3661 ggtacttttc cagaaggcaa cggaatcgat tgaccaacaa aagcgatacc tttatttaac
     3721 aatggttttc tttgattatt cccttgttgc ttactcaatt gtgccaatga ttgtacctgt
     3781 tgttttggtc ttactgaatt catggacgaa tttggaatca aaccatttga aagaccagaa
     3841 ttccactctg ttttcgattt ggtagaagac atttcaggct ctaccccatg ataacgaaca
     3901 tgacgaccac tataaggcaa ggcttgattt gaacgcccac ctagttcaaa gggattaacc
     3961 acttgcctcc cttttaccga ttcttgtggt gtacgttttt gttgaaactt ttgaaatcca
     4021 tgtatcgctg cagaagcgag tggattcgtt gctcgataca cattccctgc taatcctgca
     4081 cctttgattg ccaaattttg tccagcagtt gatagttgac caggcagttg tttcatttga
     4141 gacaaagaat gacttgctat gtttttcgtg ttgtttatag tttgatctgc agctcctaac
     4201 cctagtttct ttaataaatc tttccgtttt acaatgagta aaattgatag gagtgctttg
     4261 acaaacatat ttcctaaaaa accaaaaaca gtattcattg gaattaaatc gtctactatg
     4321 ttatagatca aaaatgccac caaaataatc acactgtaaa aagatttttg aaacatcaag
     4381 cccaaaaagg actttaaggt attcatgagc atataatcaa atactggaac aaaagaagcg
     4441 agtaatgaaa aaggaatcac aagtaataaa cacaacacca acatttgaaa gataaaacga
     4501 atcatgccaa tcactacaag tggtaatcca atacttagat taagcaatgg caccatagaa
     4561 ccaacgactg ctttataaac agctttagtt ggttgaatat agacacgata agccttcttt
     4621 ttccctctat cttctcctgc agcagtggtc acttttgtct caatatcaga taattcttca
     4681 cttgacgctt ccttggctag aaattcttgt ggttcaatct ctgcttgata caattcttct
     4741 aaatttgttg tcccataatt taacaatata tacggatcaa caacagccgc tttataataa
     4801 agttggcgca ctttttgaat ggcttccttt tgtgtcgttg gcactaaaac accattgaca
     4861 tcttttgtcg ccaattcatc tagactattt gtagcagaaa aaataaagcc ttctacctcc
     4921 acggacaaat catttaaccc tttggtaaaa gcttctcctt tactaggtac gttactacca
     4981 tagccaaacc aaaccattga aaaaatgaca acgaagagaa atcgaataaa caccattttt
     5041 gcgtaatgtt tccctctaaa tatacgaaga gtaaatagat ataacacata caagacgaca
     5101 atccccgcac caataggatc gtatattttt gaataaatag tatccgaaaa agtgaaaaac
     5161 tggtgaatta actcgttcat tgcagctcct tcatagagct tctcaatgac aaaatcaagg
     5221 ccttgccaaa tcaatttact taaactaaag aacatattcg tcatcatttt tagaaaatct
     5281 gtgccatttg ccccaaataa cattttgcct aatgaaaatt cactatcaga taaataagaa
     5341 gaaaaggtat caatatgaaa tttcccaatc tcatagatcg attgatccac ctgcttttct
     5401 gccgcataca ccaatccacc aacaaaaagc agtccgaatc caatcgttaa aaaaaacaaa
     5461 acgcaccatt tcttttcctt atgctttata taagccattg tttcacctca tttctattgc
     5521 atgattaacg tatcctgttc ttctacttga gagtgacttg ttttttccgt tgtttgtaat
     5581 gcagcggtca tttcttcaaa caaactgtgt acattgagtt ttcctacacg tccataggga
     5641 tctaacatcc aacactgtcc tttaatcatg tggctcattt cagctaagtt cgcttcattt
     5701 acttccaaac gtaaatgatt caaaatcttt tctcgctcac tgtcttcatc aaaagcaaag
     5761 acgacaccaa aattcccttt ttctttatct gaacctaagt caccaataaa ttgtgtatct
     5821 aacactaatt gattcatctg ggaacgcccc acacgtgcca ttgctgcaat aatactacgt
     5881 cctgcgttcg aattattaaa tacccatgct tctgtaaaat aaatcgctgt tttcttacta
     5941 ctatctcgtt ttccaaacat ttcacaaaaa cgacctaaac taatcatcaa acacaaagac
     6001 tttcgatccg catctgtata tagttctggt cggacattgt catttggtaa tttcaaatct
     6061 tggatctcta aaatcgttgt tttggcatta aagtctaatc ccgggttttc tccataagaa
     6121 aatcctaaac gtaaaatcga atcttctacc gtcaagcgta aaaactcacc atattctcga
     6181 acctcttttt gttccatttg ttctaaatag tcgaccacat gcatcaagcc aacttgttgt
     6241 ccggcttctt tttgaactaa aacttcttgg attcccttta ataccgcccc ttttaaaagg
     6301 tgattggtac ctaacggact taactcatca atcatatcct gggctacttg tttcgcatct
     6361 actcgggata aacaaacaat tggatctaat acaccataat tacggggatt tgtagcatct
     6421 aacgtgacat aactcatttt ttgcaaatgt tctacaaaca aagggtaatg tttctgataa
     6481 tacgggtctt gaatgagttt cgtaaaccat ttgcgctttt ctcctttggg atccacaaat
     6541 aaactttgta cattcataaa cgaactatac aaaaagagca agccaactaa aaaggtcttc
     6601 cctttaccgg tttttccagt tactgctaca tgaggcgcat caaacaacgc acctttgatt
     6661 ccttccgcaa tggctagaag attcaaaaag acaaaacgat ttgacgcata aattaattct
     6721 tctaatggtg ttttttctcc tcgagaacca ccatccccta atgtatcttg ccgaccaata
     6781 taccagccgg attccatacc taattctgat actgctgaaa acaaccattc gccaactgct
     6841 tctacagttg ttcggtgctt ccaatgtaac tgccgtccca atggttgccc taataaacat
     6901 ttatagaaca attctcgttg atcatttttt gcacggtgga tatggataat cgtatgaagt
     6961 tgttgaataa ccgttgcact attttgacga acttcttcaa ccgtttgacc gtatacaata
     7021 aaacatgcga tccaatcgac gacaggttgc ccattatcta ctcgttgctt taaatgtttt
     7081 actaatcctc gaacagttaa taagcgttca ctaggttctt catcattttg aaccaattcc
     7141 ttatgctctg ttttgaaccg ttccgatagc caattcagtt ttaaaccaat acctccctgt
     7201 cctttcaagc gtggatagtg aattttcatt cgcaattctg ctgggaaaga aagatcctga
     7261 gcaaattgaa atagatcaca atttctcata ttttcaggtg tttccccaat aggcaacaat
     7321 gctaaataac tactccctaa ctcatcttct aattgaagaa ggcctttatg ttccttcggt
     7381 atacgtacat acgttccttg attgtctttc agtacttccc aattattttg ttcttctact
     7441 ttgtgagcca ttcctcgaat atattgcaga cgattgagat acaacagctc tttttttact
     7501 aagggacgaa tagaaaagct ataaagtaat tgtgtcactt cttttacata aggatcactt
     7561 tcagaaaaca aagtttccaa atcatctact tctaatccaa ataagcgtgc taaacttcct
     7621 gctaaatcta accagattag atttattaca tcatgaaaac tctccaccgt tgctttttct
     7681 ttgactttga ttcctaaaat aaatgaagga cgatagactt gaccttctcg ttccaacaat
     7741 gtcactgttt cttttgcata atacgcacca attgcttgac tatcgggaga aaatcccttt
     7801 cctaatgtgt caaacttcgt ccgtaaattc attgttcgtg gatacatcac caactccaca
     7861 tcttcataat ctgctaaaag caacagtaaa cgagccatca tttcttttat ttcctgtgtc
     7921 ttttttggtg tgttttttga aattgttcgt ggaaaaacat gatagtaggc ccagatacaa
     7981 tcctctccat ttaacaaaca atttttcttg aatgctttta ctggttcttt tacaagcacc
     8041 aatgaatttc acctcccaat aaaaaaggaa caaccagttg attgtccctt tttctaatcc
     8101 tctaaataaa catacttatt cacaaaaagt aattcttcca atgcattgtt tgataccttg
     8161 tcccagttat aatacacaca atcaccattc atcatacgaa tcatataaca aaaccaatcc
     8221 cataaataaa aatgcacttt ttttccatca ggttttagtt ttgccagtgc taacacacat
     8281 aaatagggga gtcctatata aaaaacaact cctgctggtg gaaaaaaaga aatgacccaa
     8341 ccaataaatg aacgtaaaaa taaatattca aagattacga ccaacaaaaa agttgctgca
     8401 ttgactaatg caactggaaa tggaagacgc caattgcttg taatctgttg tacgcgataa
     8461 ggttccgcat aaacaatcga ataatcataa ggttcccaat ccacttcact atcctcctaa
     8521 tccataaata atttttcaat gattttacca aacgcatcaa atacacgtgt tggatcattt
     8581 aagaagaaat aaactgcggc acctgctgcg atagatatta atgcatttcc tacttttgat
     8641 ttcataaaat ttcgcaatcc cagataaaca accaccatta ttaaaaaata gccaccttgc
     8701 gttaatataa aatctacaat tcccttcagt gtcatttccg ttcttctcct ttcctatcca
     8761 accaattatg tgttaatttt tcaacataat actgatttgt ttttttcgta atgacaatcg
     8821 tcatttgttc tttttgtaaa agtgacgtat caccatctcg aaagacgacc gttgctttta
     8881 caataaaagg ttttttcgtt tctttataac gcaatacttt catctctact tgatcaactt
     8941 tagctgtttg attgagtcct tcaggttctt tcatcatgta tgacatatct tttgcattgt
     9001 ttttcgcata cttatctaag aaatcttcca caaatccttc aacatctttc gcttccgacg
     9061 catcaaccgt ttcgtataat gtgtcatcat aagtaatcat tcgttgtttt tctgtattct
     9121 ttggtagtgc cgtaaaataa ggataagcaa taatacgaca gcccttgttt gaaatagaaa
     9181 agggaagatt gagtacttgt gtgattgtca cacttgtttc tttcttactc tctggattca
     9241 tcgagcgaat atcataagtt actttataac tagcagttgg aatggtttca atttctttta
     9301 atccaataaa cgaagcagca gttaattttc gaacacctga aacacttcct ggttcttgaa
     9361 taaagttttg tgcataaaac tcttttttta atgtttcttg acgttgttgt tcttgttcat
     9421 gattatcgaa tgtattcata tacaaagcaa caaaacgatt cataaaggat tcaatttcag
     9481 gtgtgtacgt taattctgac cgactttgta cttctttctt ttgtaattgt tggatttcat
     9541 ctttcaaaaa cgacacttga accttcgttt tcatggattg aaaaaatgcc aagccccctg
     9601 aaaaacaaag aaataaaacc aaacaccaaa tccatttcac tgcttttttt gaatggattt
     9661 tcttttgctt cggtactttc tctgtataaa ctttttcttt ccgccgtagt tgtcgttttt
     9721 tcataagcta ctccttctag tatgttttta ctagtacgta cgattgacaa cgataagctt
     9781 gtttttctga aaaatagtcc attcaattac cctcattttc tattcctttt ctctttcttt
     9841 tatatccaac aaatccagca atacttgtca acactcctcc tagaacagaa agtgcaacat
     9901 gtatttcttc accagtcttt ggtaagactt ttgtttttcc atctgctgtt ttgatttcaa
     9961 tattgccact tggaagtttc tttacaggaa tcgacaaatc ctttaccaaa ttcccttcac
    10021 tatcttggat taatggaata ccatctttta caccaacaat tgtttgatca cctgctactt
    10081 ttaatggggt ggttggtgtt ggaatatggg tcaattcctc tacattacta gtgacaatcg
    10141 gtacactcat tccttgagaa gtattcgtcg taatttcacc gcttggggtt accgatactg
    10201 tgacttcttt tgctggaaca ataggtttat tttcttttgg aattgcttct gatttttcaa
    10261 caggttgttt cggttcttct ttttgttctt cactggtaga cgacatcgtt ggctgttccg
    10321 tacttgacga atcagtattc ttttcagaag aactggttgt tcctgattgt tccggagttg
    10381 tgactatact tgaatccgtt gttgttgaac tatgcacacc ttcttcttcg atcgaatcag
    10441 tcggtaaagt tgattcagga aactctggtt ctggtgtcac tggctctttt tcaggtttct
    10501 ctgactgcaa ttgttcaggt tctgctggtt tttccgttgt tcctccattt agctctgtct
    10561 caacatctgg taatccttcc cctggcaatg tttctacaat ttctgactta ggtgtttcta
    10621 cagtttcatc tgcataaacc aatgaactcc ctaattgaaa cgaaaataat gaaattccta
    10681 gtgttgttac gactgctaat gttgtttttt tcatgattat tcctccaatt ttttatgcaa
    10741 ttcgtgtttt gttcatagac gccgcactaa tattatctga acgataaaag aactcccgtc
    10801 cgttttcttc taacccaaca tatggattat ttttgaataa ttgaatcaat agttcactgg
    10861 tgttgtattt tggatagttt gcaaaaaaat aatactgtgc ttgtcgccca tccgaaaatt
    10921 caacaagtaa ttcataacga taccgcttca atccttttcg ttgttttaaa ggatctctat
    10981 accaacttgt ccatcgttcc attcgtggat agagatagac acgatacaca tagaacaatg
    11041 ccataccaca taccaccaaa aataataatc gccacataaa aattccccct tttcttctac
    11101 ctttcttgaa tcatcgaaca ttcataccct ctatagagat ttgaaaaatc catcatcaat
    11161 tgttcatata aacgttgttg caaatagcga ttgtttcgaa tacataattc ttcatagcca
    11221 tcaatcactt gatcttcttt ttcatagcat cttgtagctc tatgacgctc agcttgcatg
    11281 acataaccaa actctgcacc atatcgttct actgttaata actgttgact acgcaataat
    11341 gcaatataac aatcacaaaa atgttcaacc gacatttgat ttttctcacg tttgtgaaat
    11401 aggtattctt tagaaagcac attattcgtt aaacaatctg ataaatagcg ataatctggc
    11461 ggtcggttat tttcatctgt gaatttttcc cataataaac tgatttttat tcgttcagcg
    11521 gctagatagt cttgttcgcg tggataaaat tcatcaatcg cacaaagttt tttatcacct
    11581 gaataaacaa atccatataa tgcgaaacga actcttcctt ttcaattggc tctcctaaaa
    11641 attccagtga cataccatct tctccttcct aaaaaaaata aaaaagcaga aaccgatttg
    11701 gcttctactt ttctttcctt ttatagctct tttttacgga agaatccgca aatgacattt
    11761 tttcaaaaaa tcaatcggca caagttcacg aaaacttgga gcaattttgg atagaatcac
    11821 ttccccatct tgtaaaatac aatcataaaa atcatcagtt acttgatact tacgcttctt
    11881 acaataattg ataaaatagt agcgatcatt ttctatttcc attcgttgtt ctactaagcg
    11941 catacgaaca tcatcataga aataagccac aaatcgccct tcaagttcat gtttttcaat
    12001 gggatactct tctggtaaca atctattttt tcgtcgttta tacattgctg gataccttag
    12061 aagaagttca tctaaacgtc tgtcagtata tggtaactga cgaatcaacg attctaatcg
    12121 ttctttatac agatagaata attggcgcaa gcaatagtgt accgcctctg gattttctaa
    12181 tactgttgga tcacgtgacc aatacgcacc aacataaatc ataatttttt tcatttagac
    12241 aatcgcacca gcaatactaa tgaccgatcc acaataggta gaacaagtgg catagcgacc
    12301 aatttcctct aattctatct caatctcttc taaagaaaga tatgtctgtt cttctgttgg
    12361 ctggcgactc atttcccaag tgacagtcac caatgaaatt tcttccataa gaggtttttc
    12421 gctgctaagt gctgccacac agttttcata tatcttcatt ttatcgcagc cttttttaac
    12481 cgctaaaatc gcaggtattg gcaatgaaaa tcgataaaag cgaatgaatt ctagccttcc
    12541 tttccttcgg ctaaattcca actcttcgag ttcttgttcg tatcccttac gaacttctaa
    12601 taagcgtgca atttcttgcc agttctcttt ttcctgataa cgtccagctt ttaccgcttg
    12661 ttcaatataa ggaactcgaa aacgaaaata agacaaatca accatctttt catttaatgc
    12721 ttcgattgca gcctttaaat ccaacaggcg cagccattct gttgtcgtat atgcattcat
    12781 gcaattttcc ctcctgacgt tcccatccat tctagatagt tttgttgctc gtcatttagt
    12841 tcggcattat caatcccttc ttgtacaaag tctcttccta aaatacgatc tgcttcttgt
    12901 aacaacttta atgtcggagc tacttgtttc aaaaaccagc gttctgaacg atctaatgag
    12961 aatttttctg gctttactgt aaaatccaat tcttccatcg aacgaaataa ttttgcccat
    13021 cgtttatcaa caatccgtgc atcactttcc agtctatccc aaacaataag ttttgcatta
    13081 attaaaccaa cgccaatctc tcctaaacgt ttcggtgaat cacctaaaat aaatgcttct
    13141 actgcttgtg ttgctttctc attggctaac cgtaactcat atcgattata aataccatat
    13201 ttcgttaatg cttctaattc tgaaattttt tctcggttcg caatttcaaa acgtttctca
    13261 taaaaattaa aaaagagagg actctttcgc gatccaaaat acaaagataa tccttcactt
    13321 cgaaaatagc catcatctaa aaaacaaccg ccaatattct ggaatctctt aaagtccatt
    13381 tctaataaac ctgctttttg tttttctagt aattcaaaga gatcgaaatg ttcttccgtt
    13441 tccaatcccc aaagttcatc taacgcaata tcgaatcggc tacattgcac acgtgtataa
    13501 ccagcttcca actttccttg atttttccaa atggaatata gccatgttcg ccaatctgtt
    13561 tgttgttcac gcatgacttc ctcatattcc cggcaacctt gtcctgaaaa ttgcacaagt
    13621 acattttctg ttacagttcg ttttgaatgc cagaacaccc agatattccc acgaacagaa
    13681 agacagtcaa aaagatacaa gcccgttgga cgtgtttcaa attctgtcag atccattcgt
    13741 aatacacgtt gaatcactgt tttgtagtct gtcccaaaaa aggaaagacg caaataatca
    13801 atcattgctc gaagatctat tccgcttttc atatacaatc gaaagcgttc cttgattcgt
    13861 tgttcaaaat cctctgggat ttttcttttc ccttgttcca tctttctaat gtaatctttt
    13921 gagcagccca tcaattgcgc aaatcttgct tgtgatagcg gctttttatc tccacttata
    13981 ttatgaattg cacttaactt ctttctgtag ctttttagct caatcggtgt cattttctcg
    14041 ctccttttat aaaataacaa aatcgatttt ttttaaccct ttagaaacac cgatatatca
    14101 actagtcccc ctcgtttttt gggaaaagta gtacaactgc tgaccttaga taatcacttg
    14161 atacaaaagg gtttataaaa cttttttcta tgtgttatgt tatttctgcc ccccatatta
    14221 tatatcgggg ggctttacac aaagaggccg tgcgcaagcg cacgacctct ttgtgagctg
    14281 cacacgctgc tagcgagtgc gcattatagg cccatgcttc gcagccttaa tgcgcaccgc
    14341 caggcagagt gcggctacca gactacgtct agagccctcc cgatagataa taactacaaa
    14401 attaaatgat ctaattttgt tatttcacta tatttttcag gagaaaaaac gcgataaatt
    14461 gacaaagtta aatcctcttc taaatctact cgataatgat acaatcgagt caatcgttct
    14521 atatcttttt tcgttagcaa tacttgatta ataacttttc ctgtaaaaac acgttctttg
    14581 cgcaaaaagc ggtaatcagt tacttgttca atacgattca accaatcata aaacgttctg
    14641 gaagaaagag aaggattaat cattcgtatt gctgtagcta ctctataata acaactctct
    14701 gccatttaac ttcacctcct tagttgatta caaaatatgc gcatcagaaa aacttgttcc
    14761 ttttggatct tttgctaaat acatttggcg ctctagttgt tcttgtaaca actgattatc
    14821 atctcccatc gtttgatctt gactatcctc ttttactgta aaatcataga caatcatttc
    14881 agtaaatttc tcaaattctt tgataaagtg ataatctttg taaacaaatg gcgcaaaaaa
    14941 ctctacaggt acatcaaatc ctggaaaagc atatccacga ccaggttgat ctttgttgta
    15001 aaatggctta tttttaaaat cttctccaaa agtcattaaa taaccaatgg gagacaatcg
    15061 accaacagaa atacggaatg ttaaattatc ccgaacacca cttggaaaat attctgcatc
    15121 tggtcgttgc attgctaaaa taatgtacat ccctgcttga cgacccttta atactaatgg
    15181 ctgaatcaat ttatcgactc tatttctcgc ttccatatcc aacgaaccat aaaaagctgc
    15241 ccattcatca aaaaagataa aatgcggtgg taacccataa taagcataat ttttccctgg
    15301 ttcatattta ggatctttca tggttttcat ataggcatac cgttgattca tttcttttaa
    15361 tgcatttcgt aaacaaataa tcatttcttc atttattttg taaaacacat gtcctttgaa
    15421 tacatcgacg tcacctaatg ctgctaaatc tgccgctttt ccatcgcaaa tatctactgt
    15481 cccaacactt aaacaagctt gaattaaaga atacaataaa taggttttcc caccaccgat
    15541 tgcaccagca atcaacatat ttggtgattg atcatatacc caatccaaag ttttcattaa
    15601 atgaatacgt ccattttctg ctatacattg atcaatagga atccgaccag aatctgaaag
    15661 gaatacataa ttgattcggg ctttttctcg ttcttcacga attaaatcac gcaaaaatac
    15721 ttcagataac aaatgaccca tttttaaaaa tcgttcgtga aaacgcccac catctaaagg
    15781 aaaagaaacc actaattctt gtttcttttg ataaaaataa acttttggta atctcatttt
    15841 ttcccttgtt tgtttctcac taacttgttt ctttgtataa acaaaccgat tgtcaattaa
    15901 cattcgaaca agtaactgac gccaataaac agaggcaaaa tatccttctt ttagtcgcga
    15961 ccaatgcatc aaccctaaaa ttaacccact aaccagcaac aaatacaaca caatacttat
    16021 tccaggaata agatacattt gccaattcga aaacgtactt aaacgtggat aaaatacttt
    16081 ccaactaaac caacctaaca gaatcaaaaa tggaaacaaa aagaaaccat aatagcaaaa
    16141 ccataaataa cgattgaaat agcgaattcg ccgaccacga tacttgaaaa aatgagcttt
    16201 caattgtcac attcctcctt tctgttttta cttttcctgt gctaaaaact tttgacaaaa
    16261 agcaactaat acaggaatat cacatttagt aaaaaaacat aatcgaaaaa gatttaatcg
    16321 atttttataa gggtgtgcac acatattaca tattcgtact atacgaatcc aaatcacttc
    16381 ctgatgatct tgtttccact ggtacggtaa tgaaaaacga acaagatcac gtaaaaacat
    16441 acactgccca ataaccgcac aaatatatgc atttgtttgt acaaaaatcg ttggattgtc
    16501 tccaaaatac tcaatcaatt gttggatatt ttgataacaa gcacaatctc tcaaagtatt
    16561 tgattggttt tatctgaa
//
LOCUS       DAYYHX010000046        16538 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000046 DAYYHX010000000
VERSION     DAYYHX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.046"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(455..1084)
                     /gene="udk"
                     /locus_tag="ACHA6Y_001123"
     CDS             complement(455..1084)
                     /gene="udk"
                     /locus_tag="ACHA6Y_001123"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM8987233.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1168..1863)
                     /locus_tag="ACHA6Y_001124"
     CDS             complement(1168..1863)
                     /locus_tag="ACHA6Y_001124"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM8987234.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1876..2592)
                     /locus_tag="ACHA6Y_001125"
     CDS             complement(1876..2592)
                     /locus_tag="ACHA6Y_001125"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM8987235.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2922..3527
                     /locus_tag="ACHA6Y_001126"
     CDS             2922..3527
                     /locus_tag="ACHA6Y_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM8987236.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3852..4175
                     /locus_tag="ACHA6Y_001127"
     CDS             3852..4175
                     /locus_tag="ACHA6Y_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8987237.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4357..5727
                     /locus_tag="ACHA6Y_001128"
     CDS             4357..5727
                     /locus_tag="ACHA6Y_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM8987238.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5857..7158)
                     /locus_tag="ACHA6Y_001129"
     CDS             complement(5857..7158)
                     /locus_tag="ACHA6Y_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8987239.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7388..9379)
                     /locus_tag="ACHA6Y_001130"
     CDS             complement(7388..9379)
                     /locus_tag="ACHA6Y_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM8987240.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9523..9789)
                     /locus_tag="ACHA6Y_001131"
     CDS             complement(9523..9789)
                     /locus_tag="ACHA6Y_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8987241.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10299..12425)
                     /gene="pnp"
                     /locus_tag="ACHA6Y_001132"
     CDS             complement(10299..12425)
                     /gene="pnp"
                     /locus_tag="ACHA6Y_001132"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM8987242.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12613..12882)
                     /gene="rpsO"
                     /locus_tag="ACHA6Y_001133"
     CDS             complement(12613..12882)
                     /gene="rpsO"
                     /locus_tag="ACHA6Y_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM8987243.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13133..13696
                     /gene="def"
                     /locus_tag="ACHA6Y_001134"
     CDS             13133..13696
                     /gene="def"
                     /locus_tag="ACHA6Y_001134"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8987244.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13949..14470)
                     /locus_tag="ACHA6Y_001135"
     CDS             complement(13949..14470)
                     /locus_tag="ACHA6Y_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM8987245.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14473..15315)
                     /locus_tag="ACHA6Y_001136"
     CDS             complement(14473..15315)
                     /locus_tag="ACHA6Y_001136"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM8987246.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15308..16033)
                     /locus_tag="ACHA6Y_001137"
     CDS             complement(15308..16033)
                     /locus_tag="ACHA6Y_001137"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM8987247.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16209..16505
                     /locus_tag="ACHA6Y_001138"
     CDS             16209..16505
                     /locus_tag="ACHA6Y_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987248.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
BASE COUNT         4654 a         3460 c         3057 g         5367 t
ORIGIN      
        1 tgcgacaaaa gagcttcttc aaaaaaataa gtcgaaatct cacacaaatt cgagaaataa
       61 ttttcgtaag tttcttctta tttattgcat ctgaccactt ctgttacaac ctctcgttaa
      121 acggtgttca aaagcttgca tctacgataa taagcccaac ccaacgaaaa acgtgaggag
      181 ctgttttatt tggatgctct tatcgcttga tgcaaagaca gcttttttac aacctctact
      241 tcacggtgct caatcagctg accttcggta ttagctcaaa aaacggtaaa atataaacag
      301 ctattttccg tttcttcttt ttaatactta ggtcaagatg ctgttttcac aacctcttgt
      361 tataaataaa aaaaacagtt ggctggtgac tgttcaaaaa gcagaatcct tgcttttcaa
      421 acggtcgcag tccgttcaac tgtttcgctg cgcactattt atggtttaag aaactagcta
      481 cttttgttgt aatcaaatca atagcaacgt ggttttctcc gccttctgga acaatgatat
      541 ccgcatagcg cttcgttggt tcgataaatt gatgatacat cggtttaaca actgtcaaat
      601 attgttcgat cacggagtcc aatgtgcgtc cccgttcttc catatcccgt ttgatacgac
      661 gaatgatacg aatgtcatca tctgtatcta cgtatacttt gatatccatc aagtcacgca
      721 gacgttcgtc ttctaaaatc aggattcctt ctagaatgat cacttctttt ggttcctgaa
      781 tgatcgttgc ctgacttctt gtatgcgcta catagtcata cacgggtttt tcaatggctt
      841 tatagttcag taagtcgcct acatgctgaa tcagcagatc attatcaaag gcaaatgggt
      901 gatcgtaatt tgtgttcaat cgttcttcaa aactcaaatg gctctgatcc ttgtaataag
      961 aatcttgttc aagcatcatg atggaatggt ccgggaagtt gttgaaaatc gcacggctga
     1021 cacttgtctt tccgcttccg gatccacccg tcacaccaat gataattggc ttgctttttg
     1081 tcattgacta aaacctcttt cgtatccgtt ttttcattct aatctattat agcgaaaaaa
     1141 cacgaaaatg ctatattttt ctgaaaatta atccttttca atttccaata atgaataaag
     1201 ttcctctaat gaatcgattc gataagtagg ctggatttct tcgatttctg gtaatttatt
     1261 tctattgaac caaatcgtat cgatccctgc catatttcct cctttgatat cagaagtcaa
     1321 agaatcacca atgatcacag tttgcttttt atcaaaatta ggaatgtggg aaaagacatg
     1381 atcaaaaaat tctttcatcg gcttttgcgc tccgacagct tctgagacaa agatatcctt
     1441 gaagtaatca agcattcctg catctgttaa tcgccgatgt tgcgtgtgcg acacaccgtt
     1501 cgtcacaata taaagatcag catgcggatg gacctttttc aggatttcta gacttcctgg
     1561 aacaagatca tgaccttggt tcaagtagtg gcggtagcgt ttttccattt gatcactatc
     1621 tactgttttc ccaaattgct ggaaaagtaa cccaaaacga ctgcctatta tttcttctcg
     1681 tgatagtctg cctttttcaa actcctgcca taaatgatga ttcaaagcct tatagctggt
     1741 ttcgatttct ggtgtcaatg tgattccttg ttctgcaaac aaagcactta aagcattggc
     1801 ttcagttaat tgaaaatcta acaatgtatc atctacatcg aacaataaag ttttatattt
     1861 catctttttg acctcctatt taaaataaat acttaacttt ttcatctctg ctaatgtcaa
     1921 cgatcgatac tcgcctactc ctagctgttc atctaattcc aatggtccca tgcttagccg
     1981 tttcaatgcg gtcactttct ttccttgggc taagaacatc tttttgactt gatggaattt
     2041 tccttcttgg atcgtcaata aaaccgtact ttggtgcttg tctgctttga gtatttttaa
     2101 atcagccggc ttacattgat acccatcagc gaatacgatt ccattttcaa atgctgttac
     2161 gtctgccggt gtcacccttt cgttcacaac tacttgataa gtctttgaaa cctttttttc
     2221 tggaagtagc atttcatatc ccaattgtcc attcgaagtg atcagcaata gtcctgttgt
     2281 atctctatct agtcttccca ctggatataa tggttggaaa cgatcggcac ttgcaatcag
     2341 atcgattact gtcgtctttt ctggatctga tacagctgta acagcttttt caggtttatt
     2401 gagaagataa taggactcat ttgtttctaa gtgttcacct gctacttcga tttgatgcag
     2461 taaactatct acatttcgat tttgtcgaaa ttcgactgtg ccatctatca tcacttgctt
     2521 caaagcaaac agccgcttca tttccttgcg tgtcgtttct aatttttctt ctattaattt
     2581 atctaaacgc atgctgtttc ctttcttatt actactaatt aattatacct tgaatattct
     2641 tgcttgcaaa tacatcatca tagttattcc ttgacttaat taacagttca cgttacggtt
     2701 aaatagtaaa gtgaattgga tttctcatac acagcgtaat gacctggtgg agaaaagttg
     2761 aatcctgact ttctaagggg agatgcgcgc atctctcctt ttgcgctttt ttaagcgccc
     2821 tgccttcgat gatgtgcacg agattagcct ttagaattca cttaaataaa atctgtccgt
     2881 taccaaagca gacagtcaca ttttaaggag gaattttggt tatgtcagta tcattagaag
     2941 taagtaaaag agaagtacgt ccccgttcat taagaaacaa acttcgtcac gaagggaaag
     3001 ttccagctgt tgtttatggg tataaagtag aaagtacccc tatctcattc gatggtttga
     3061 cactaagcaa attgttgcgt gaacatggag cgaacacagt catcacgatg gacatcgatg
     3121 gacaaaaagt caatacattg gttcacaaga tccaaacgaa cacttttacc aatcatttcg
     3181 aacatgcaga atttctatct gtaaacatgt cggaagaaac agaagtcgaa acagaagtcg
     3241 ttcttgttgg cgaatcagtg ggtgctaaat ctggtggagt gttagctcaa aacttgtata
     3301 cagtagtcgt atctgctaca ccagacaaat tgcctgaacg aattgaagtt gacgtgacaa
     3361 acttggaatt aggtgattct attacagtag ccgatcttcc aaaaaacgac gattacaaat
     3421 tcgtgaccga cagtgaagag caaatcgctg ctgtagtcgc tcaaagtgaa gaagaaagca
     3481 caggtgaagg tggcgaacca gaagtgatcg gttcaacaga agaataatac tgaaacaatc
     3541 aggtggtgtt agaagcggcg ggtcagcagc ttttaacacg ccttttttat ttctaatcgt
     3601 ttattttcat atggggcttc cacttttgta aatggcttta caaacgaaaa atcctaattt
     3661 accaagcttt tcctcgcata cttttatcgt acctttataa aaatttatac tttgaaaaaa
     3721 agtattcaat tattgttgca tttttcctga ttctttatta gaatgaaccc tcgttgaagt
     3781 aagtatttct agtttatcct aggattaatt ataaaaataa aacattcgtt gattcgaagg
     3841 aggttaaaca gatgtttgaa agttttgata gcagccgcaa ccgttacgct tcagttggtg
     3901 tcgtatcaag tttgccagat gaattgattg acagtatatg gtttattatt gacctagatc
     3961 ttaagggagt cattccatta gaaaatatat taacctttga tttgatcgaa aatcgtggaa
     4021 aagtgacgat gcatttttct caagcaggaa gcaatgtgga aatggatatt gatttgccgt
     4081 ttgcctattc gaaccaattt cctgctcaag tatacgccta tgatgacggt acgagagaaa
     4141 cgatcctttt accaagtgag attagacaat attaaggtga tagataaacg tggatgtccc
     4201 ttattttaca gaccaggcca agttgttgat tctttgactc aattcttggc ttctttttat
     4261 ggagtttcat aaattatcat tgatttctaa ttggtattct caggataaac gctatatact
     4321 actaactaaa ttttaaagat ataaggagtt taaaagatgc gcgatttaac aaaaggaagc
     4381 cccgctaaac tgattttgat gttcactgtt cctttactga tcggaaatgt ttttcagcag
     4441 ttctacaata tgatcgatat gatcatcgtg ggtcagacgc taggtaaaaa tgcgctggca
     4501 gcggttggtg caactggaag cctgacattt ttgatcattg gctttgccca aggtttaaca
     4561 gctggtttag ccattattac cgcacaacgt tacggtgcaa aagattatcg gggtctgaag
     4621 aaaagttttg ctgctagtgt tgtcattagt cttgtcgtca ctgtgatttt aactgtccta
     4681 agtctcctgt ttattcgtcc gatgcttcaa ttgatgcaaa ctcctcctga aatacttgat
     4741 caagcacaaa catttatttc aattatttta ttagggatct ttgcatcagt cagtttcaat
     4801 ttgctttcta atgtcattcg cgcacttggt gacagccgca caccactatt ctttctgatc
     4861 attgcggtca tcatcaacgt cgtacttgat ttgatcttta ttatctattt tggcatgggt
     4921 gtggaaggtg cagctattgc tacagtaatc gcacaagttt cttccagcgt actgtgtcta
     4981 gtgtatatca agaaaaaaat gcctttgtta cagttacgca aaaaagattt caaatttgaa
     5041 aaagaagagt ttgctgtaca cctaaatgct gctttgccaa tggcgtttca atcttcgatc
     5101 atcgctgttg gtgctatcgt gttacaagca gcactaaata gtctagggac agatgtcgta
     5161 gctgctcaag ccgctgctgg tcgtatcgac caatttgcaa atcagccgat gatgtcattt
     5221 ggtattgcaa tggcaacttt cactgcacaa aactatggtg cgaaagaata tggccgaatc
     5281 ttgcaagggg tacgtcaaac tttactgatg agtgtcggat ttagtctctt agcaggtgct
     5341 atcgtcattt tcttcggtca tttctttgtt caattgtttg ttagcccatc agaaacacgt
     5401 gtatttgaat tagcacaaac ctacttcaat atcaatgggt ctctttactg gatcttagca
     5461 gtcctgttca ttttacgcta cacacttcaa gggcttggac aaagcaagat ccctacgatc
     5521 gccggtatga tggaattgct tatgcgttct tttgcagcta tcgttcttac aggaatgtgg
     5581 ggatacccag gagcagcagc agcttctcct ttagcttgga tcggttctgt tgccgtgttg
     5641 ctttactctt atatgcgttc gatgaaacaa ttgaaacaca tgggtgaaga ggaacatttc
     5701 acattagaac atgcagaagc aagatagcct tgattaaaag aaatcgttgc ggctaaaaat
     5761 gaaaactttt attgaaaata tcttcttcgt ttaccgaaaa caggtttttt agataacaaa
     5821 aaggctgtga caaaaaatgt cacagccttt tttcttttat tctatttttc tttccgtgga
     5881 ctctactttg aaataatcga tatgtgcatg ctggtattct aataccgtga aacggaatga
     5941 tgcaatctca atgattgttc ctgcttttat ttctttatat cggctagaag caaagcccgc
     6001 aagtgtagta aaattcgttt cttcaaactc tggaatctct acatgaaaat aacgctggaa
     6061 atcatctaat gttagtttgc cggagatgat ataagagcca tctgactgtt ccgctatcaa
     6121 tgcttcgtcc aaatctgtct catctcggat ttccccgacg atttcttcta aaatatcttc
     6181 aatcgtcact aagccgcttg tccctccgta ttcatctttt aagatagcaa tatgctttcc
     6241 cttttttcgc atgatcgtca ataatttttt tatagggatc gtttcagttg ttacgatcgg
     6301 ctcttcaatc aactgttcta ctgtcagctg gtcatcttta taagattgct tgatcaaatc
     6361 tggcaaggtg acataaccta aaacattgtc ttttgattct aagataaccg gatagcgagt
     6421 atgtccttgt tcaatcgaag cttttatcgc ttctttgacg gtttctgaag cttcgaatac
     6481 ttgcatggac gtacggtcta cttgtatatc ttttgccaag gtatcatcaa attcaaacac
     6541 gttttccaaa taatgatatt cttctttggt caactctcct ttttcataag aattcaatgc
     6601 gactaatgtc agctcttctt gagacaatgt ctcttctcct tcagatacga gacgcacacc
     6661 taatagcttc cctagccctg cagctgaatg attcaatacc caaataaaag gaaacattac
     6721 tttataaaaa taatgaagtg gtttaactac gaacaagacg accttctccg tattgacgat
     6781 actatagctc ttaggaagca gttcgccaat aaccacatcg atatacgtaa ctaaaataaa
     6841 tcccagcgcc acagagattg tactgctaat agccgaacta aaaggtagtt taccgatcac
     6901 tggttctagt aaagccgcga cagatccttc accgacccac ccgatgatca aaccggttag
     6961 cgtaattcct aattgactag ctgataaata gttatctaaa tggtgtgtaa catgaagagc
     7021 tagcttggcg ttctttacat tttccttttc tagttgttcc aatcttgatc gtctgatttt
     7081 cactaaagca aattcacttg ctacaaaaaa tgaagtaatt gcaatcatca aaaataaaat
     7141 aatgaaattg ataaccatat tctcccgcct ttctcttttt ctattgtacc ctttttctac
     7201 aaaaagattg aaaataatgc gtattcatat ttaggaataa aaaaggagat gcggcatttt
     7261 tcagccacat ctccttatca cttcaaaatc attgcgttac ttgttgctaa acagcttttt
     7321 catgacctct gataaacggt gttcaagtag ctgacattcg acattagttc aggtcaaagc
     7381 gctactttca caaccgctct ttcaaccatg ttttccgagt atttccaatg ataagcggaa
     7441 cagtttcatg ttttacttcg ctgtactcta gcccaaacca tgtttctggc gaggtagcga
     7501 tttttttgat tgccagtttt gcctgcatga tttgacgttg ccattttcct agagttgtca
     7561 cattggaaac tacttcatca atcaggacaa attggaaatc gccaacttct cgccctggtg
     7621 tcagagagta tctttgtggc tgttttggca aacggccttg tttcatcaaa tcatggatga
     7681 cttggcgaac atatacgttg atttcctgtt ggaccctgaa acctaagaac agattcactt
     7741 ggacaataaa atcagtgtcc atcatatcta cttgatattc tttcgtatat ggctcatccg
     7801 tcacttccac atgaacaaac caatagactc gtgctctttt aggccgttta tctaaaatcg
     7861 aatagatgaa ctgtcggcca actttatctt cagccatatc aggaaccaag aagaccacat
     7921 ttgtctgata gattggtaaa gaagtatcat cacgtaattc tctaagctgc gggatataat
     7981 ctctcaaaga cacatcctcg gttgacgatt cgcgaataat gttcccttgt tcccaaataa
     8041 acatcacgac taagatgatc aaaccaatca gaacggctac aaaaccgcct cggaagaatt
     8101 tgacaacact tgagataaag aaaacgactt ctatcgcacc gaagaaaaag gtaactaaat
     8161 atgcaagcca acgagaatgc ccattttgga taagataaaa gtaaagcagt gctgttgtca
     8221 tcaacatcgt cacagtgatc gatagaccgt aagcagcttc catatgatga gacgttcgga
     8281 atgtgacaac cactagagaa caggcgatcc ataaaatcag attgactgct gggatataca
     8341 tttgcccgat actgttccca ggatacatga tctttaaccg aggcagcagt ttcaacttga
     8401 ttgcttctga aactagcgta aacgaacctg taatcaatgc ttgcgaagca atgactgcag
     8461 cgactgttgc aaaagcaacg ccgatcacga tccaactttg aggcatcatt tggaagaatg
     8521 gattcaggtt cttgatttgc tgtgcttcag ggttttgata aacattcaac aaccacgcag
     8581 cctgcccaaa ataattgata accaaacaaa ttttgacgta aggccagctc agacgaatat
     8641 ttcgtttgcc gacatgtcca agatcggaat aaagcgcctc tgcacctgtc gtagccagga
     8701 aaatatttcc aagaatgaac aagcctaatt tgttatctgc actaaaaagc agctggatag
     8761 cataataagg attcagtgca cggataactg tccagtcttg actgaagttc attaatccca
     8821 ttacgcctaa aaaagtaaac caagcgaaca tgattggtcc gaatgctttt ccgacaatat
     8881 ctgtaccaaa gcgttggacc atgaataaaa caaaaataat aactaaagtg ataatgacga
     8941 tcacgttctg gttattaccg aaccggtcaa aaaactgcgg aattcctcgc aagccttcaa
     9001 tcgcagtcgt taccgtcaca gcaggggtca ataccccatc tgctagtaag gcagctcctc
     9061 cgatcattgc aggaatgatc aaatatttcc caccttttcg gacaagtgtg tagagtgaga
     9121 aaattccacc ttcaccatgg ttatctgcat tcaatgcaat cacgacatat ttgattgttg
     9181 tcagtagagt caatgtccaa aaaatcaatg agactgatcc gataacaaaa tcaggattga
     9241 ttcctgacaa tccgccatta tcttcaacaa tcgatttcat aacgtaaagc ggacttgtcc
     9301 cgatgtctcc gtagacgact cccatcgcta caagcaggcc agccattgat atctttttgg
     9361 tattgtgcgt tgttgccacg tttttccctc atttcttgtt atatggatat cctttaggta
     9421 agcttgaatg atagatgata ttcttatcca tgacacccgc tttcctaaat tgcttctaat
     9481 tatccaagta acacactgaa gtgtcaactt atttgaataa aattattctt tctttggacg
     9541 ttctctgata cgtcttgcca caaatgaata aagagcaagg caaaaaattg cacccaataa
     9601 tgctggtatc aaataaaaac cgccgagttc cggtcccagt ggtcctagta aaaattcgcc
     9661 gagccaacta cctaaaaaac cgataatgat atttccagat acgcctcccg ggacttcctt
     9721 tcctaaaatt gcgccagcta tcaaacctaa tataccgccg ataaataacg accataaaaa
     9781 attcagcatc tcctttccct cctttatcca tatttctcac ttttatttta cgctttattc
     9841 agaaggactc caaaaatatg cgccaagaaa ctccagaaaa aaagaagttg taacaaaagt
     9901 gttcagctta tttccgtaga agctgcttct ggaacaccgt gaatcagagg ttgtgaaatc
     9961 agcggtttga cctgagtatt agagaaacaa acggaaaaat agttcctcat attttgccgt
    10021 tttttgagct aatatcgagg gacagctgat tgaacatcgt ttattggaga ggttgtaaaa
    10081 aagctgttct gcatcaagta ataagaacag ccaagtaaaa acagctccta atgtttttaa
    10141 cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc gaaaaaaaag
    10201 gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt ccccctgtca
    10261 ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc atttttcgtt
    10321 gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg tttatcgatt
    10381 tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac tttattgaca
    10441 cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat caagttgacg
    10501 aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc aactttgact
    10561 tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc atcagctgaa
    10621 gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc atcgatgatt
    10681 ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg ttcgatctgg
    10741 atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc aatcgttgaa
    10801 gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc ttcagttagg
    10861 atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat accatctttt
    10921 gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg gatatcagtt
    10981 aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc gactggcgct
    11041 ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact tgcttgggaa
    11101 gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg aaattcttct
    11161 tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc gatttcccga
    11221 cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa attgtagtgg
    11281 tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg ctctcctaga
    11341 ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa acctgatcca
    11401 tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc caatttacgc
    11461 ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt ttcaagatct
    11521 tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc tgcaaatttc
    11581 tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc gcgagcaagt
    11641 ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa gatttctttt
    11701 ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc tactgcttga
    11761 acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa cagcaatgca
    11821 ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt gatggcttgt
    11881 ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac tgttggattc
    11941 aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc gtcaaatggg
    12001 atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc tggcgagcag
    12061 tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa gccttctgca
    12121 aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc cgtacttgga
    12181 cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat tttttcttca
    12241 taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc cacagctgca
    12301 cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg ttttgctagc
    12361 tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac ttgtttttct
    12421 gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa atgaaaactg
    12481 ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct agcttgatca
    12541 cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga atcccactct
    12601 ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt aacgttgaac
    12661 gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt tcattaggcc
    12721 gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt ggttgatttc
    12781 ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat ggcgagcgta
    12841 ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca cctctttcga
    12901 ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg caggtcatgt
    12961 gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt agttaatcat
    13021 taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt ggtaaactat
    13081 aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt ccatgattac
    13141 aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa aagaagtttc
    13201 tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt tcttaaaaaa
    13261 cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg gcttagctgc
    13321 tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc ctgacccaga
    13381 ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga gccattctgt
    13441 tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag aagtaccagg
    13501 atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg gtgaaaaaca
    13561 caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg accatatcaa
    13621 cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa aagaaggcgt
    13681 attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa gaaagcttta
    13741 taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc actaatcgaa
    13801 atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg atacgcatct
    13861 tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga tagaatagtt
    13921 tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct gcttcagcta
    13981 gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta tgcagatgga
    14041 tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt ctcataaagg
    14101 aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg taaaggggat
    14161 gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc agttcttctt
    14221 ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta ttggtttctt
    14281 ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca gctcgtagta
    14341 aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca aacttgctag
    14401 cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc cgtgtctctc
    14461 cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt gtttcatcaa
    14521 atcaacatac gtgtctgtta ttgtttcctt atatccctta tttttcagca gactaacaac
    14581 tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt cttctttttt
    14641 cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa ccaactctcg
    14701 atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta gttcttcctc
    14761 tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg gaaaagattt
    14821 ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac ccggcgtgac
    14881 gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg cgtactccag
    14941 accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct ggacattcgg
    15001 agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg tacaattgaa
    15061 atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat tgataactgg
    15121 ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt gggatcgcag
    15181 ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca tacggtcggc
    15241 aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt ctttttcggt
    15301 tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca accgctccgt
    15361 cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg attactaaga
    15421 ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact tgaaaactcg
    15481 ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc tccatgactc
    15541 catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct ccgcatgctt
    15601 gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat tttcgacaat
    15661 gacattgata cagactccca tgaaaattga cgagttgcgg gcttttagcc tttgcgtgca
    15721 aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga tgtgctattt
    15781 ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca aattgataga
    15841 atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc tgctccatcc
    15901 cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg gatacccctg
    15961 caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct tcgtctactg
    16021 ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg aacaaattta
    16081 ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga gcaatttatc
    16141 acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata aaagtgaggt
    16201 gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt atcgcctggt
    16261 tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat ggaaatcttt
    16321 gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca ttagccgtat
    16381 atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta atattagcca
    16441 tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag aaagcggcag
    16501 cttaaatgat cgagatggaa ctggacacag gaaatttt
//
LOCUS       DAYYHX010000047        16191 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000047 DAYYHX010000000
VERSION     DAYYHX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.047"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..2116
                     /locus_tag="ACHA6Y_001139"
     CDS             2..2116
                     /locus_tag="ACHA6Y_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8987249.1"
                     /translation="MYQNTYESIESSYLTDKKNLLRQVKTNVEWKIRTIEYSFSTYGS
                     TKNFSDIFKHPLTYTDYSTYSEIRKELNFIETIVMDDNNYDLVSLAGKWGVINGSLSQ
                     LTEEEVEKYQQKYINNKNNLFWQKKNKGIEMVISLPMFEKEKYALGIATIEKHTIEQV
                     VGESGEDILSIENEVGNLFTTQTVKNKEQPTVLTDVDLDYNQLKVIREKDKTYIVLKS
                     DYNNWIYRLEVDSSAIGAMIRNLRIGLVAVSLTLVGLIGLLSYLFSDRFARPISQIQE
                     RLNLKSKGFSGKELDLVAQSVSRIIGEKEALSAHLVTQKPQLETLFVLSLFRNRVERR
                     ELDQRLQQFGYSSQNDCYYTALVQIDLLEDSHGGERDLLLLAINNMITEIVPKENRML
                     PIVLNEEMQATIYRMDKDDAEASKKVMEYCRLIQKRVKEYLNLTISIGISNRFETLNE
                     SKQSVDRAKEALYYRVNTGPSSIIFYQEIVPAAHEKTLIRYPIEEQNRLFEAIRSGNQ
                     EVKNLVHELIDALFAQNKNPLSREVVSVRLINELVQLGQLLGVDSKNFDNMKQIYVKA
                     LNNYHPKELEKLVLEQLVLPITECSQAITDHEFKNLSENIMHIVYTEYDRDLSLDVIA
                     DRLHYNPNYLSNVFKKETGEKFGDFVQNHRLEVAKKWLKETNLSVKEIAERLQYRNPQ
                     NFIRFFKKKESITPGEYRKKYL"
     gene            complement(2574..3446)
                     /locus_tag="ACHA6Y_001140"
     CDS             complement(2574..3446)
                     /locus_tag="ACHA6Y_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8987250.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            3746..4720
                     /locus_tag="ACHA6Y_001141"
     CDS             3746..4720
                     /locus_tag="ACHA6Y_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8987251.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            4742..5434
                     /locus_tag="ACHA6Y_001142"
     CDS             4742..5434
                     /locus_tag="ACHA6Y_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8987252.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            5400..6908
                     /locus_tag="ACHA6Y_001143"
     CDS             5400..6908
                     /locus_tag="ACHA6Y_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8987253.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            6982..8085
                     /locus_tag="ACHA6Y_001144"
     CDS             6982..8085
                     /locus_tag="ACHA6Y_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8987254.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            8088..9425
                     /locus_tag="ACHA6Y_001145"
     CDS             8088..9425
                     /locus_tag="ACHA6Y_001145"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM8987255.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            9412..10290
                     /locus_tag="ACHA6Y_001146"
     CDS             9412..10290
                     /locus_tag="ACHA6Y_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM8987256.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            10287..11210
                     /locus_tag="ACHA6Y_001147"
     CDS             10287..11210
                     /locus_tag="ACHA6Y_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM8987257.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            11207..11617
                     /locus_tag="ACHA6Y_001148"
     CDS             11207..11617
                     /locus_tag="ACHA6Y_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8987258.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            11621..13189
                     /locus_tag="ACHA6Y_001149"
     CDS             11621..13189
                     /locus_tag="ACHA6Y_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM8987259.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            13429..14847
                     /locus_tag="ACHA6Y_001150"
     CDS             13429..14847
                     /locus_tag="ACHA6Y_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8987260.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            14859..16169
                     /locus_tag="ACHA6Y_001151"
     CDS             14859..16169
                     /locus_tag="ACHA6Y_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM8987261.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT         5374 a         2646 c         3412 g         4759 t
ORIGIN      
        1 catttaccag aatacctatg aatcgatcga atcgagttat ttaacagaca aaaagaattt
       61 gctcaggcaa gttaaaacta acgttgaatg gaaaattaga acgattgaat attcgttttc
      121 tacgtatggt tcgaccaaaa atttctcgga catattcaag catccgctga catatacaga
      181 ttactccact tattctgaaa tcaggaaaga actaaatttt atagaaacga ttgttatgga
      241 tgataataat tatgatttag tcagtttggc tggtaaatgg ggagtaatca atggatcgtt
      301 gagtcaactg accgaagagg aagttgaaaa gtatcaacag aagtacataa ataacaaaaa
      361 taatttattt tggcaaaaga aaaacaaagg aatcgaaatg gtgatttctt tgccaatgtt
      421 tgaaaaagaa aaatatgcat tgggaattgc tacgatagag aaacatacaa tagaacaagt
      481 tgttggtgaa agtggagaag acatactttc tattgaaaac gaagttggca acctttttac
      541 tactcagaca gtaaaaaata aagagcaacc aactgtatta acggacgtgg atctagatta
      601 caatcagctg aaagtgataa gagaaaaaga taaaacgtat atagtactga aatcagatta
      661 taataattgg atttacagat tagaagttga ctctagtgca attggcgcaa tgatccgcaa
      721 tctgagaatc gggctagttg cagtgtcttt aacactggta ggactcattg gtcttctttc
      781 ctacttattt tcggacagat ttgcccgtcc gatttctcaa atacaggaac gtctgaatct
      841 taaatcaaaa ggattcagtg ggaaagaact agatttggtc gctcaatcag tcagtcggat
      901 cataggagaa aaagaagcgt taagtgcaca cttagttacc caaaagccac agctggaaac
      961 gctatttgta cttagtttat ttagaaatcg agtagaaagg cgagaactag atcagcggtt
     1021 gcagcaattt ggttatagtt ctcaaaatga ctgctattat acagctttgg ttcaaatcga
     1081 tctgttagaa gatagtcacg gtggggagcg tgatttgcta cttttagcga tcaacaacat
     1141 gatcacggaa attgtgccaa aggaaaatcg aatgctgccg atcgttttga atgaagaaat
     1201 gcaagccact atttatcgaa tggataaaga tgatgcagaa gcaagcaaaa aagtcatgga
     1261 gtattgccga ctcattcaaa aacgtgtaaa agaatacttg aatttaacta tcagtatagg
     1321 catcagtaac cggttcgaaa cgctgaatga aagcaagcaa tcggttgatc gggcaaaaga
     1381 agccctgtat tatcgtgtaa atactggtcc aagttcaatt attttttatc aggaaatcgt
     1441 tcctgctgct catgaaaaaa cacttattag atatccgata gaagaacaaa atcgattgtt
     1501 tgaagccatt cgttctggaa atcaggaagt gaagaattta gtacatgaat tgattgatgc
     1561 attgtttgca caaaataaaa atcctttgag tcgggaagta gtttcggtcc gcttgatcaa
     1621 tgaactcgtt caattaggac agttattggg agtagattca aaaaactttg ataacatgaa
     1681 acaaatttat gtaaaagcac tgaacaatta tcatcccaaa gaactagaaa aacttgtttt
     1741 agaacaatta gttctgccaa tcacagaatg ctctcaagca atcactgatc atgaatttaa
     1801 aaatttgtcg gagaatatta tgcatatcgt ctacacagaa tatgatcgtg atctgtcttt
     1861 agatgtgatc gctgataggt tgcattataa ccctaactat ttaagtaatg tatttaaaaa
     1921 ggaaacagga gaaaaatttg gtgattttgt tcaaaatcat cgtttagagg tagcaaaaaa
     1981 atggttgaaa gagactaacc tctccgtaaa agagattgca gaaagattgc aatatcgaaa
     2041 tccgcaaaat tttattcgtt tctttaaaaa gaaggaatcg attacgccgg gagaatatcg
     2101 aaaaaaatat ttgtgaatca agaggctgtt tttcttgtgg actgattcaa gcaataaaag
     2161 aagttagaat aaccctagta tttgtattta tggatagtta aatttcaagg ctaagcacgc
     2221 aaaaagaact gtgacaaaag tcttgtcaca gttctttttt acgaataaat ggcgttcaag
     2281 agacaggcta tcgacaataa ggcataattt tgtgaaatat gggaagctat ttttcaaaat
     2341 cgcttcttat tgctcctgtg ggttactcgt cgcaatgttt gtgagagttt ttatcatcgc
     2401 tcaaggctga acgtctcttt cacaacctct gatacacggt gttccaggag tagattcttc
     2461 gaaaaataag ccgaaatttc acaaaaattt aagaagcaat tttcgtaaat gccttcttat
     2521 ttcttgaagc tgaccacttc tgacacaact accattttgc gttcagtaca taatcaccct
     2581 ttgacagaac ctacgagtgc tcctttgaca aaatatttct gaacaaaagg ataaacaatc
     2641 agcataggca ctgttgcaat aacgataaca gccattttaa ctgtttgtgc tggtggaatc
     2701 acatcaacgg cactactttc tgcttgcatc ccacttgata caatgacgat ttggcgtaaa
     2761 agaatctgta tcggccattt agatgaatcg ttgatataca aaatagcagt catataagtg
     2821 ttccagtaag taacagcata aaaaagcgag atagtagcaa tagacggtaa cgctaatggc
     2881 agcataattt tgaaaaatac accaaaatct gtgcaaccat ccatttttgc tgattcttcc
     2941 aaactatccg gcaaggcttg gaaaaagttt ctcataatga tcatattcat tgcattaaca
     3001 gccacaggca ggatcatggc ccagtaagaa ttgaccagcc ctagagaacg aacaactaag
     3061 aacgttggaa tcatcccccc gctgaacaac atagaaaata caacaagaaa gttgacgaaa
     3121 cctcgaccaa acaaatactt tctggaaaga ccgtaggcca tcagtgaagt tgcacacata
     3181 ctgacgatcg ttccgacacc tgtaacacca atcgaaacag caagtgctct aaaaatggtc
     3241 ggcgtagaaa gaatgtagcg atacgcgtct aaagaaaagg tagtaggaaa aaggataaac
     3301 tttttcgcga cgacttcttg tgtagaagca aaagatgtag cgatgatatt taaaaaagga
     3361 acgatacaga tcaaagctag caaaattaga aacgtattgt tgaaaagtgt aaaacttctt
     3421 tctcctatag agactttctt tttcatatag ataggctcct cctcaaaata aattaaatgt
     3481 ttctgtttct atatgttcaa tttatcatcg gtgaaagatt acgtatataa tcatttccta
     3541 tgctaagtgt cagatagaaa aaaatagaag gtttgctatg cgtagtttat ttgtgaaaaa
     3601 atacacttta tcgttttgta tagcacaaaa atcattgcta tgtaagcgtt tttacttacg
     3661 gtaaaaatga ttatatacgt aattcagata gttatctatc atgaagtcat aagcagcaaa
     3721 cattcacaaa actaaggagg ggcttatgga aagtgtaaag aaacaagaac catttcaact
     3781 gacgctatcg aaaaaagaag aaaagctcag aagacgaaaa aaaataattg caggaatcaa
     3841 aacgaataaa tttttatatg tgatgattct gccgggagtc ctttatttct tgatttttag
     3901 gtatcttcct atggggggac tcgttattgc ttttcaagat tatcaaccat ttttaggaat
     3961 aatgggaagt ccttgggtcg gtttcaaaca tttcattcgt ttgtttacag aaccgacatt
     4021 ctttacatta ttaagaaata cgttgatctt gttcggatta aatatcttcg tgttttttcc
     4081 gatgccaatc attttgtctt tactattaaa tgaagtaacg aacaaacgat tgaaaaacgg
     4141 gattcaaacg atcatttaca tccctcactt catgtcttgg gtaatcattg tttcgattac
     4201 ttatgtattt ttgaatgttg acggaggggt attgaatgaa ttccttgcaa caatgggatt
     4261 tgaaaaaatc agttttctaa catcccctga atggacacgc acagtatata tcggacaagt
     4321 gatctggaaa gagttgggct ggtctacgat catctacctt tcagcgataa ctgcagtcga
     4381 tacacagttg tatgaagctg cagaaatgga tggagcagga cgcttacgca aaacatggca
     4441 tgttacattg ccggcaattc gtccagtaat cattaccttg ctgattctaa aaatcggtca
     4501 gacattagac ttagggttcg agcatatgta cttgttattg aattcattga atcgaagcgt
     4561 tgcagaaatt ttcgacacct atatttatac agctggattg aagaatggac agttaagttt
     4621 cagtacagca atgggattgt ttaaaggggt cgtaggattg tttatggtaa ttttagctaa
     4681 taagattgcc aaaaagctag gagaagacgg cgtttactaa gatagaaaga gagggaaaga
     4741 tatgctgcag tcattagata aaataaacgg aaggctaaat cgtattttac agttggttta
     4801 tttgaatttt ctgtggggcg gtttagtcat cgcaggtttg ggagttttta ctttcggtcc
     4861 tgcatcgtat gcgatggttg cagtgattag aaaatggctg cgctcaaaag aaacgtttcc
     4921 tttgacgaaa acgtacttca catattttaa agaaaactat cgtgaaacct tgctgatgag
     4981 ctggatatat ggcgccatcg ggattgtttt acttgtggat ctttctttca tacacaattg
     5041 gtatcttagg atcgctttgt atattgctgc gttcatatat gtgctctcag cactctatat
     5101 tttcccaatc atggctcatt atgattggaa aggatatttt ttcaaaatga aaatggcatt
     5161 tttattcgga ttctcacatc ttcattactc attagtgttg atattaagta tggtagggat
     5221 ttatctgatg atcatccaaa caatgccagg tgttttaacc tttatcggtg gcagtctctt
     5281 cttttccgca gtcacgtgga cagcaaattc tatattttgc catttggaat ggaagaactc
     5341 atatacagaa aaaacagaag aaaacaaaaa tattattaaa acagtgaagg agaaaattta
     5401 tgaaaaaagc aacgctcgta aaattaggtc ttagttttgc aggaattcta gcacttgccg
     5461 catgcggcag cagttcaagt aacagttctt cagagacaga agatggaaaa gctaaaatca
     5521 cttggttaaa tattttgcat catgcttcac cgcctacaga cacgattgtg aacttgatcg
     5581 aagaaaaaac cgatacggaa ctgacattca actggattcc tgatgcttca aaagaagaaa
     5641 gattaacaac tgcattggct tctgatgaac taggagatat cgttacactt caaattgcga
     5701 tgttgaaaaa ctcttctgtc cgcaatgcgt taaaatcagg cgtattctgg gatgtatcag
     5761 aatatttaaa agactataaa aatttaagtc aaatttctga agatagaatc gaagctgcga
     5821 gtatcgacgg tgccttgtac ggcgtaccga tacaaaaaga ctacgctcgt gctggcttag
     5881 tcattcgaaa agactggttg gataatctag gactagatgt tccaactacc atggatgagc
     5941 tttatgaagt agctaaacaa tttacagaaa acgatcctga tggaaatggt gtggacgaca
     6001 cagttggttt tggcgatcgt gcgcctagtg atattcgtta taccagcttt aaacttttca
     6061 cttcttattt tggagcacca aatggctggg ctgtagacga taatggcaaa ttcactccag
     6121 aatttgatac agaagagtat atgaaagctt tgaatttttc aagagatcta tttgagaatg
     6181 gctatttggc acaagacttt gcagttaccc aaaagactga tcagcaagaa caatttgcac
     6241 aaggaagaac tggaatctac actggaatgg tggatatcaa gaatctgaag acgatgggtc
     6301 aaggtattca agatgatatg gaattagtgc ctgtaaacaa gatctcagat ggtgaaggcg
     6361 gcggttatca tgtttggtca gaaggaaatg gtattggcgg cttgctagca tttcccaaat
     6421 ctcaagtaaa agacgaagca caattgaaga aattacttca atttgttgat gatcttttag
     6481 aagaagatgt gtataaattg atgactggcg gtatcgaagg aacgcattat gaattgaatg
     6541 atgatggttc tatcgaattt atcgatatgg atctttggca acaagaggtg caaccgttat
     6601 caagctctcg tcctagtgaa gtgacgttct ctttcaaaga tacagaccct gaaaaagaat
     6661 tctctaatca attgatcaaa gaaaatgaag aatttgctgt aatggatcct tctgtgccat
     6721 tggattcacc gacgaacaat gaagtaggat cagaaattga aaaaatcgtc attgatgcta
     6781 cggtgcaata catcatggga cagcttgacg aagatggatt caaaaaagct gtagaagact
     6841 ggaaagcaca aggaggcgat cgaatcacac aagagtacga ggaagcatac aaagcagcgg
     6901 cacagtaata tagacgattg ggacaaatac ttcttggtat ttgtcccaaa tcctatttta
     6961 atttttaaag gagagcaaat aatggaaaac tggtcaaaga aattagcaaa aagtatcatg
     7021 gaaagaacac ctcgcttata tgaagaaaag tggtataaag gaaaatggtc ttatgactat
     7081 ggcgtcgtac ttaaagggtt ccaattactt tgggaacgaa cccaagaaaa aatttatttt
     7141 gatttcataa aagacaatat cgactatttc gtgcaagaag atggaacgat ccgtggctac
     7201 agcgttgaag aatacaatat cgaccatgta aatacaggaa agcttttctt tttattgtac
     7261 aaagaaacag gcgaagaaaa atataaaaaa gcagcagaac tcctttccca gcagttggca
     7321 aaccatccac gcacatccga aggagctttt tggcataaag aaatttatcc ttatcaaatt
     7381 tggttggatg gcttatatat gggttctcct ttttatgctg aatttatcca gaattttagt
     7441 gaagaaaaag attaccaaga tgttttgaga cagtttgaaa tcagttataa acatctattc
     7501 gatccaaaaa caaatttgct gattcatgca tgggatgaaa agaaggtcca accatgggct
     7561 gatccaaaaa caggtctatc ccatcatttt tggagccgat cgattggctg gtatttgatg
     7621 gctgcagcag atacctatga attacttcat gaagaaacag attgttcttt gctgaaagaa
     7681 attctagaaa agacactggc tgcattactt ccatatcaag aagaaaacag cgggacatgg
     7741 tatcaagtaa caacagaaac aaaacggaaa ggaaattatc tggaagcttc cggcagtagt
     7801 atgtttgttt acgcaatggc taaaggaata cgtttgggac tgctgaacaa agaagaatgg
     7861 cttccacaag tgaagaaatc acatcaagga ttacttgatg aatttgttct ggaaacaaaa
     7921 gaaggctggc tgaacttgaa taaaaactgt caagtagcag ggttaggcgg agcagatcag
     7981 cgagatggtt catatgctta ctacatcagt gaaccaatta tttgtaatga tcaaaaaggc
     8041 gttggtgcat tcttgcaagc attgattgaa gtggaggcat tataaatatg tatgatattt
     8101 tgacatttgg cgcatcaatt gatgaattga atactgaagc aatacaacaa gccattgatg
     8161 ctgcagcaag tgacggaggc gggaccgtag ttgttccagc aggtgaattt ctaacaggag
     8221 ctttattttt aaaaagcaat gtcgaacttc atttaagcgc aggagcggtt ctgaaattct
     8281 ctgacgatcc aaaagattat cctgttgttc attcacgttg ggaaggagtc catcgaaaag
     8341 tttatgcttc ctgtatctat gctcaaaatg tagagaacat ctctgtcact ggtttcggta
     8401 cattagatgg aaatggaaaa aaatggtggc atacttttag aaatgaacca gataatctcg
     8461 cttatcctcg tcctaaattg atgagtttcc ataactgtca tcgaattact gtgaaagata
     8521 ttaaattgat ccagtcacca agttggacga tcaacccgat tctttgcagt aatgcaacgt
     8581 ttgataactt gacgatcttg aacccagcgg attcaccaaa tacagacgga attgatccag
     8641 aatcatgcaa aaatgtgcgt attagtaatt gccatattga cgtgggagat gactgtattg
     8701 caatcaaggc agggactgaa gatacgtacg aaaggatcgc ttgcgaaaat atcacgatca
     8761 caaattgcac gatggtccat ggacatggcg gtgtcgtatt aggaagtgaa atgagtggta
     8821 gtattcgtaa tattaccatt tcaaattgta tcttccaaga aactgatcga ggaatacgat
     8881 tgaaatctag acgaggtcga ggcgggatcg ttgaagatat tcgagtatcc aatatagtca
     8941 tggataatgt gatgtgtcca tttatactga atctttatta tttctgtggt ccgcgaggga
     9001 aagaaccgta tgtatgggaa aaaaaggctt atccgataga tgaacggaca ccagcattca
     9061 gaaggatcca tttttcgaat atcacggcaa ggaatgtcca tgcttctgca ggttttattt
     9121 atggattagc ggaacaattc atacaagaaa tcacatttga caatatcgat gtctcgatgg
     9181 cccaagatgc agttcccggt gttcctgcta tgatgacagg aatagaggaa atgagcagac
     9241 aaggatttta tattggcttt gcgggtaagg tttcctttaa ccagatgtcg attgaaaatc
     9301 acgagggacc agcatttcta ttagaaaata ctaaagaaat cagtattgag aaattccaat
     9361 caaatcaatc agaaaatatg gatgaactgg ttgttcgtaa aaactttgct tatgcagttg
     9421 aatgattttt taggtgctcc atctagcaga cataccccta gtggaaaacg attataccga
     9481 gaaaaacaaa acggttttat gcttgaatac tatcaattgt acttaaatga attggaaaca
     9541 gtaccagccc tctatgctta tccagaaaaa gaaggaccgt ttccgccggt cctttacctg
     9601 cattctcacg gcggagattt ttcagtcggg aaaagtgaat tgatccatgg tgctccttac
     9661 ttagcttctc catcttttgc agaagtactg acaggaatgg gatatgctgt ttggtcgatt
     9721 gatgcttggg gatttgaaga acgcggaggt atttctgaaa gcgagttgtt caaacaattt
     9781 ttattaacag gaaaaacgtt atggggaatg cgtatttatg atgtgatcag cttgatcgat
     9841 tatttggaga caagggaaga cacagatact caacggctcg caacgattgg tctttctatg
     9901 ggaggcttat taagctggtg ggtgacagca ttggattctc gtgtcaaagt ttgtattgat
     9961 ctagcggcac aggtggacat cgaaatactt ttgctacatc gccgcctaga tcatcatggg
    10021 ttttattact atgtgccaaa tctattgaca gcttatacaa cgttagcaat ccaagaaaaa
    10081 atcgctccgc gccctcgtct tagcttagtt ggaaaaaacg atacgatgtg cttggacgaa
    10141 ggcgtactga agctgagaaa agggctggac aaaaagtatc aatcaatggg tagtccagaa
    10201 aacttttctt ctttttcttt aacaggcggt catatggaaa cacaagaaat gagaaatatt
    10261 tggaaacaat tcataaggga gcatttatga ggatgaaaga caaactgata attggaaaga
    10321 atgctataga taccccgacg gattttgaaa ctatccaaga aggaatcgat tatttggcag
    10381 gcctgccgga tagccatcca aaggtcttga tagtaaaaga aggaagatat gatgaatata
    10441 tagaaagcag attatccaat tttaaacttg tgggagcagg aacagtagtt atctccggta
    10501 atcgttttgc aaaacaggag tatcttgacg gtagccagtt agggactttc cgaagtgcta
    10561 cttttttctt agaaggatcg aatgtcgtat tagaaaatct tcacatcgaa aatacagcag
    10621 gtccaggaga aaaggtagga caggctgttg ctttattcaa ttcaggagat cgcgtatctt
    10681 taaaaaactg tcgactatct ggttatcagg ataccttatg cacgggtccg ttgcctgatt
    10741 tgcaaaaaga tggtactcct ttttgtacac cggcttctgc caatcctgag tattgtcgtc
    10801 agacctatga atattgctgg attgaaggaa cggtggattt catatttggt ggggcagatg
    10861 ccgtttttca tcagtgtcat atccatagct tagccagtaa aaatcctgga tacatcacag
    10921 cagcatctac gccgaaaaat caaaaaacag ggtatcgttt tgaaagctgt accttgactg
    10981 cagaacctga tactagaaac gtctatttag gaagaccgtg gcgtccgtat gcaaatgtct
    11041 tatttgaagc gtgtaagata ggtgggcatg ttcacccaac tggttgggat aattgggatg
    11101 acaagaaaaa tgaacagact gtcaaattct ctgaacgtaa taacaattat gaaacatcga
    11161 tcaaaagacc tgaatggata catttagaaa agcagggggt tcagaaatga aaagaaaatg
    11221 ggaactattg ctaggaatgg tcggtggcag tctatctttg attttctttg gcgggttagc
    11281 agttacttta agtaatatgt ctgcgtcaga gtttaaaaaa agttatcagt cgcttgctgt
    11341 agatcattca actttgtctt tagaaaatac atttggatta ttacaggaca tgactggtct
    11401 gtttgccgtt gtgttgttta tttcacttgc ttttttagca gtagcattgt ttttgactgc
    11461 taaaggaaaa tacttaacaa ctgccactgg tttgtatttt ataacaggat tcatattgtt
    11521 gattggcact cagtttattg cgtttccttt tgcatttttt tatttcgcag ctggggcatt
    11581 cagtttatat cgagtaagaa tgagaaaagg agcctgaatc atgcagacta tcaaagtgaa
    11641 aaatcctatt ttaagaggat tcaatccaga tcctaatata ttaagagtga aagagcggta
    11701 ttacattatt gtctcctctt ttgaatggct tccaggaatc cgagtatatg agtcgaaaaa
    11761 tttagtcaac tggtcacata caacggatat tttgaccact caagtatctt tgcaaggaaa
    11821 tcctacaaat ggaagtatat gggcgccaca aatcagttat gctgatggat tgttttatct
    11881 tgtttacaca gatgtcaaaa gtgcaggccg tccgtttaaa gatgtccaca attatttgat
    11941 cactgcatct gagatcacag gtccttggtc cgaaccaatt tatttaaata gtaacggctt
    12001 tgatccttct ttgttccatg atcttgaaac gggaaaaaaa tggcttgtca atgaactatg
    12061 ggattatcgt ctggacacac cgaataaatc cagcggtatc gtattacaag aatatgatgc
    12121 agagaagaag aagcttgatg gtcccatcta taaaatattt gcaggtacag aattagcgaa
    12181 aacggaggca ccacatttgt attttcgtaa cgggtattat tatttgatga cggcagaagg
    12241 cggtacagga agaggacatt cagttacagt ttgccgagca aagaatatta ctggtcctta
    12301 cgagcttgat cctggttttc ctatgctgac agctagtgac aaacctgctt ctacattgca
    12361 atgtacagga catgggagct tagtacaaac cccgtcagga agatggtaca tgacatatct
    12421 ttgtaccaga ccattaaacg gtgcggctat tttgggaaga gaaaccgcta ttcaagaagt
    12481 gtattggacg tccgacgatt ggttaagact taaagatagc cagacaactc cgatggaaga
    12541 aataatgatt gagacaaatg aagaggtaca acaagaaaaa gatcatcagt ttatggatac
    12601 atttgaagga gagctaaaga agaactggaa tgccagacgc attttgccaa atgctgattg
    12661 gtgtgataca aaatgtagac caggtttttt acgtctgatt tccggtgaat ctcttcaatc
    12721 taccttcgaa catcatttgt tagctattcg gcaaacggat tttacttttg atgcggagac
    12781 agcatttgaa tacatgccta ataattttaa tcagatggct ggactggtac tgtacttaaa
    12841 tgaatccaat tatatttatt gttatgttac ttggaatgag gacaagggaa aatgcctacg
    12901 tatgatagaa tcagaaaacg gagtagcaca aatagaagat tggacgattc cactcacaga
    12961 aaagaagact tttttgaaag tcactgtaaa gaaagagaca gcacagtttc atttgaaaga
    13021 cgtatcaact gaagaatgga agaaagtagg tccgcaaaaa aacatgtgta tactctcagg
    13081 agggtttact ggaaatttta tcgggatagc agtgcatgat ctaaataaaa aaagaggaag
    13141 ttatgctgat tttgagtttt tcaagtatga tggaaaagat catctgtaat ttaattcttt
    13201 aataaatgag gttcactaac ttattttcac tagttagtga atttttttgt ttgtatatat
    13261 tgttatgtaa aaaaagttat caatcttatg catcaatgtt tttagattat tttttgtttt
    13321 ttatctgtca agataatgca ataaaaagcc aacattgtga atataaaaac aagaaaaaaa
    13381 gagttataat tttgaaaacg tttactagaa gaggtaggag gaattcaaat gctttcacaa
    13441 aatgctgtta cagcaaatac aataaaaaaa gaaaaaaagg aatttgggat ttccgataag
    13501 ataggctaca tgttcggtga tctaggtaac tgttttattt taggtttagt caatagtttc
    13561 ttgatgattt attatacgaa tgttcttggc gtttcagggg caatcgtagg tacgttattc
    13621 ttgatttcga gaattttgga tgcgtttgct gatgtgatgg ttggaagatt atgtgatgtc
    13681 tcagagttaa cagaaactgg ccgatttatt ccttggatca gaaaaatgaa gtatccattt
    13741 tgcttagtga ctgtcatttt gtttttgcca ttcgtccaac atcttccaat gacggtgaag
    13801 attgcttata tttttgtttc atacattgtt tatgggattt ttctctcaac aatcaatatc
    13861 ccgtatggag caatggcagc agcaattagc tcaaatccta atgaccgcac ctcgttgtcc
    13921 acgttccgaa gcattggttc tgcgattggc ggatcaacta caggattctt cattccgatt
    13981 ttaatgtata cccataacaa gagtggacaa caagtcgttt cagggaatca tttcttttgg
    14041 atttcaatcg gatgtgctgc aatagcattt gtcgcatata tgttaacttg taaattgact
    14101 actgaaagag taagagttgt caaaaacgaa aaagtatctg cttctcagtt ggtaaaaggt
    14161 ttgttagcta atcgagcact gattatttta gtcattgttg acattttcat tgtgatcaat
    14221 cagattttag ctgggacgaa tatgacttat ttattcaatg attacttcca taataaacaa
    14281 gcaatgtctg ttgctctttt atttacttac ggaacagtag tagttttagc accatttgct
    14341 acaaagctga caaaattatt tggtaaaaag gaagcaagcg tatttgcgtt acttgcttct
    14401 tcatgtatgt acttgctgat gtatttcctt catataacaa atgcatgggt atatctgatt
    14461 cttttattta tcgctacttt aggatctggt ttattcaatt tgatggtctg ggcattcatt
    14521 actgacgtga ttgattatca tcaatataag acaggcttaa gagaagatgg aacagtatat
    14581 ggagtaaatt cttttgcgag aaaagtagga caagcatgtg caggagcggt aggcggattt
    14641 atgctggcac tcattggtta ccaatcgtct acaacaggtg gagccatcca atcagtttta
    14701 gtacaagaaa ggatttatac gatggcaaac ttgttaccag cattctgctt atttgtatcc
    14761 gcaatcattt tgctagtagg ttacccactg aatcgttcaa aaactgtatt tattggtgaa
    14821 gaattgaaaa aaatcaatga aagataggga gaaacgtatt gcggacaaaa aaagggatta
    14881 tcaatgcttt gaactttaaa acaaacgaca ctcaacaaga tgatacaata atactgcaaa
    14941 aagcaatcga tcaaggtgcg aaagaacact tacctgtatt tattccaaaa ggcatttatt
    15001 tagtcggagc ccttttttta aaagataaga gtcatcttat ctttgaagaa ggtgcagtcc
    15061 tcaaggggag aacagatatc gaagcatttc cagaaatcga tacacgagta gccggcgtag
    15121 aaatgaaatg gccagcagcg attttgaatg ttctctcggc gaaagatatt cttatagaag
    15181 gaaaaggaat aatcgacggt caaggagacc attggtggga gctgtattgg ggaaaagatc
    15241 aaaaaagcgg gacaagagct gaatacgatc aaaagggact tcgttggatt gcggattatg
    15301 ctattaaacg tccacgcgct tgtttactgt atcatgcaga acatgttgtt gttcgtgatt
    15361 tgatttttca gaaatcaggt ttttggaact tgcaaatcac ttattctaac gatgtattag
    15421 tagaaaaagt aatcatccgc aacaatgatg gaccaagtac agatgggatt gatattgatt
    15481 cttcaacaaa tgtgcgtgtc tatgaatgtg atttggcctg tggtgatgat tgtattgcta
    15541 ttaagtctgg cagagatggc aatggtgcaa gagtcaatcg aaaatcatca agaatcgagg
    15601 ttgctagatg taaaatccgt tcaggatacg gtgtaactat tggtagcgaa gttagtgctg
    15661 gagttagtga cgtatatatt cacgatatcg atttttttca gtcagactgt ggtattcgaa
    15721 tgaaatcttc aaaagaaaga gggggagtga tcgaaaatat cagggtagaa aatctgaata
    15781 tgattgatgt acaatttcct ttttcttgga taatggactg gcataatgaa tacaatagaa
    15841 aaaatagcaa tgaccttgct caaatgccag aaagttggca agcagtggct gaagaaatac
    15901 ctgaatcaaa gcaaatgacg aaagtcaaag acatacacat caaaaacgta caggcatgcc
    15961 tttctccagg ttactcactt ccttcccgag cttttgattt agtagcattt cctgaaaagc
    16021 ctatagaaga tgtctgcttt tctcattgcc aaataactgc aaaagaattc gggcgaatcg
    16081 aagcagtaca aaacttatgt ttcaaaagct gtgatttatc tatagaaact ggcaacacag
    16141 ctgcaaacaa tacttttgac aatcgttgaa attataataa ggtcaagctt a
//
LOCUS       DAYYHX010000048        16171 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000048 DAYYHX010000000
VERSION     DAYYHX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.048"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(374..1195)
                     /locus_tag="ACHA6Y_001152"
     CDS             complement(374..1195)
                     /locus_tag="ACHA6Y_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8987262.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(1292..1570)
                     /locus_tag="ACHA6Y_001153"
     CDS             complement(1292..1570)
                     /locus_tag="ACHA6Y_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987263.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            1838..3847
                     /gene="metG"
                     /locus_tag="ACHA6Y_001154"
     CDS             1838..3847
                     /gene="metG"
                     /locus_tag="ACHA6Y_001154"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM8987264.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(4326..4913)
                     /locus_tag="ACHA6Y_001155"
     CDS             complement(4326..4913)
                     /locus_tag="ACHA6Y_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987265.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            5088..6410
                     /locus_tag="ACHA6Y_001156"
     CDS             5088..6410
                     /locus_tag="ACHA6Y_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8987266.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            6565..7341
                     /locus_tag="ACHA6Y_001157"
     CDS             6565..7341
                     /locus_tag="ACHA6Y_001157"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8987267.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            7334..7903
                     /gene="rnmV"
                     /locus_tag="ACHA6Y_001158"
     CDS             7334..7903
                     /gene="rnmV"
                     /locus_tag="ACHA6Y_001158"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM8987268.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            7896..8780
                     /gene="rsmA"
                     /locus_tag="ACHA6Y_001159"
     CDS             7896..8780
                     /gene="rsmA"
                     /locus_tag="ACHA6Y_001159"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM8987269.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            9231..9860
                     /locus_tag="ACHA6Y_001160"
     CDS             9231..9860
                     /locus_tag="ACHA6Y_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987270.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(9984..11093)
                     /locus_tag="ACHA6Y_001161"
     CDS             complement(9984..11093)
                     /locus_tag="ACHA6Y_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8987271.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            11302..11724
                     /gene="mgsA"
                     /locus_tag="ACHA6Y_001162"
     CDS             11302..11724
                     /gene="mgsA"
                     /locus_tag="ACHA6Y_001162"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM8987272.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      11911..12009
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            12047..12583
                     /locus_tag="ACHA6Y_001163"
     CDS             12047..12583
                     /locus_tag="ACHA6Y_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8987273.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(12623..14386)
                     /locus_tag="ACHA6Y_001164"
     CDS             complement(12623..14386)
                     /locus_tag="ACHA6Y_001164"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8987274.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(14389..16104)
                     /locus_tag="ACHA6Y_001165"
     CDS             complement(14389..16104)
                     /locus_tag="ACHA6Y_001165"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8987275.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         5143 a         2967 c         3259 g         4802 t
ORIGIN      
        1 tcaataccga cggagcagaa gaaagcaatt ccgtaagaaa gagccaagta gtagaaaaaa
       61 tgggcagaca ttcggttatc aagggataaa aaatgaatga aactgatagg agcgttaggg
      121 ttagccttgt taataaaaag tcttaatgac ttttgtttct aggaattttt cttttgaaga
      181 aaagctcact aattatgaca aaccaataaa acaatagcag aaaaagttcg tacgagcttt
      241 ttctgctatt gttaattttt tattcagaaa gtttacgaat acatcaaaat tctgaaacaa
      301 aaaacgtaac tcacaaagag ttgtttttgc gttttttctt gaatgattag gtctgaatct
      361 tttgctcaca accttattca tggtgccccg gcttgagctg atagattttt gaaggacggc
      421 cgattcctac aggacgttca ccgatctcat cgaaatattg gagcatagct ttcttaaaat
      481 tggaatgatc gatcgtttta taatcgatcc ctaagaattt agcaaatact tttcgagctt
      541 ccgtaatcgt gaaatcttta cctaaaactt gtagaacttg cggctcgtgt tccattttat
      601 tgactacacg gttgaatgct ttcaagatga tttgactatg gtcaaaagcc aaagtatctt
      661 tgccagaaga ctctccagtg actaaatcaa gcgagatagc cacttctccg ctagataaat
      721 ggatcttgtt actatgacgt tctagtgtga accagcgtac ttccttggca tcatctccgg
      781 cgattagcgg ttcttctcca ataaaggcta agtagctgac tgtgacgacc cagcctcgcg
      841 gatctcgatt aggcgtactg aatgtatgca gttgctcaat attttctttt gaaataacca
      901 caccagtttc ttcctttgtt tctcttaaga cgctttctcc agttgactca tccttttgga
      961 caaagccacc aggcaatgcc catgaatttc gataaggatg cccttttctt tggatcaaca
     1021 aaactttcag ctgatcttct tctttgttat agcacatcag tacgatatcg acagtcaaag
     1081 agggcttgtc atattctgga cgttcctgtt tttgatacca gttaagaaat tcttcttcac
     1141 ttgctttatg ttcataatat aatttttctt cttctttaga agcaaaacgg ttcatctgac
     1201 cacctccatt gacagtaaaa gtacttttta ccttattgta gcagactctg aaaaaaaacc
     1261 aaggatttac ttgaaaaatc taggttatca tttaaatatg aaaaataaat ggaagtatga
     1321 gattgaataa aaagaaaagc agataaaacg gcaatacttt ataccgctct tcacgaatgg
     1381 caaagaagcc acaaataacg atatatacgt caatgataaa acgcaaaatc caagctgctg
     1441 tcataaaact gttcaaatgc ccatggaggt taaaataacc atacatacga atataaaaat
     1501 agctgaatat taaatgtgct aatgaaagaa aagcaatact aagtgtaaaa aaaagatgtt
     1561 tcttctgcat gattgttcca gtcccttact aagatagata gtctttatta taccacgtgt
     1621 gaggggagaa ataaaaagcc gttttttagt agatatgaaa caaaacaaaa acccgcattt
     1681 attcaaccta ttcatccttt taaaggatag attgaacgta gatagaagtc gtttatccag
     1741 tctttttcat tttgaaaaaa agctaacagc atgctttttt tatggtatac tagcaaggaa
     1801 acgaattcgc atcatgcgat aaaggaggaa gagccccgtg gcagaaaagg aaacttttta
     1861 tatcactacg ccaatctatt atccaagcgg caaacttcat atcggcaact cgtatactac
     1921 gattgcatgt gacgcaattg ctcgttataa acggttgatg ggctttgatg tgttttatct
     1981 cactggagtg gatgaacatg gtcaaaaaat cgaaacaaaa gcagaagaac taggtgtaaa
     2041 accgcaagaa tatgtagata aaatggcggc tgatgtcaag aaattatgga aaacgcttga
     2101 tatcagttat gataaattca ttcgcacgac agatgattac cataaagcag ctgttcaaaa
     2161 aatatttgat cgtttattag aacagggaga tatttactta ggtgaatatg aaggctggta
     2221 ctctgtttct gacgaagaat tctttacaga aacacaactt gcagaagtgt acaaagatga
     2281 cgaaggtaac gtgatcggtg gtaaagcgcc aagtggacac gaagtagaat tggtaaaaga
     2341 agaatcatac ttcttccgca tgagcaaata cgcagaccgt ctattggaat actatgaaga
     2401 acatcccgaa ttcatccagc cagaatctcg taaaaacgaa atgatcaaca actttatcaa
     2461 accaggatta gaggatcttg cagtttcacg aacaactttt acttggggag taccagtcaa
     2521 gagtgatccg aaacatgtgg tgtatgtatg gatcgatgca ttatccaatt atattactgc
     2581 actaggatat ggctccgaag atgaaagtct attcgaaaaa tactggcctg cagatgttca
     2641 gatggtcggt aaagaaatcg ttcgctttca tacgatctat tggccaatca tgctgatggc
     2701 ccttgatttg cctctaccta aaaaagtatt tgggcatggc tggctattga tgaaagacgg
     2761 aaaaatgtct aaatcaaaag gaaatgtcgt ttatcctgaa atgcttgtgg aacgttatgg
     2821 tttagatgca ctccgttatt atttgcttcg tgctgttccg tttgggtcag atggtgtctt
     2881 tacgccagaa gattttgttt cccgtgtcaa ttatgatctg gcaaatgatc tagggaactt
     2941 attgaatcga acgattgcta tgatcaacaa atattgtgat ggactcgttc ctgactatgc
     3001 ctcacttgtc actccatttg atagtgaact gtctacgacg gctgctaacg ttgtcggacg
     3061 ttaccatgat gcaatggaaa aaatggaatt caatacagca ttggctgaaa tctgggtgct
     3121 gatttctcgt gcaaacaaat acattgatga aacagaacca tgggtattag caaaagacga
     3181 agaaaagaaa gctgaattga atagtgtcat gatccattta gctgaatctt tgcgtatcgc
     3241 agcgatatta cttcagccaa tcatgacaga aacgccagag aagatcttca atcaactagg
     3301 attggactct gaaacaatga atcttgaagg attacacttt ggcgaatttc cttcaggaac
     3361 aaaagtagtt gcaaaaggta caccaatctt cccacgttta gatatggaag aagaagttgc
     3421 ttttattcaa gaaaaaatgt ctgaaggtac tcaaacaaac gaagatacag tcaaatggga
     3481 tccagaagaa acagaacttg tttcaacgaa agaaaaacaa atcaaatttg atgtatttga
     3541 aaaagtagaa ctaaaagtag cagaagtcat caactgtcaa aaagtagaag gggcagataa
     3601 gttgctgcaa ttccgactag atgctggaga cagccaagac cgtcaaatct tgtcaggaat
     3661 cgctgaattt tatcctgatc caagcgaatt gatcgggaaa aaagtcgtca ttgtagcaaa
     3721 tctgaaacca cgaaagatgc gtggacaaat cagtcaagga atgatccttt ctgcagaagc
     3781 accagatggc tctttacaag taatcgaagc accaaaatcc atgccaaatg gctcagaaat
     3841 cgcatagaaa ttcaaagggg gtatgacact agttgtgtca tacccccttt ttccgaaatt
     3901 tgagaagcaa ttttcgtaaa ttccttctta ttacttgaag ccaatcatat aatacagaaa
     3961 cagagaacta tccaaaataa atcctagaaa aagaagtacg agcttcgctg atttttgtta
     4021 atttagaata atactaatag gaatcgtaaa aacagaaaaa aagcataatg ataaagggtc
     4081 gggcagattt taattgattc atctggtact cttttttatt gtccttacat tcataaagtt
     4141 tgaatgcaat gactttatta catctatatt tatatctaac agtagcgttg atgtgttaat
     4201 tataagttct atcactttat gttcaattag tttgtgctct tatatgtgtg attgaaaata
     4261 tagataaaaa aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa
     4321 tcctatcact tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga
     4381 gcagtaagca gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca
     4441 taatgttgat agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag
     4501 ccagctataa accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa
     4561 ggatagaaat aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg
     4621 atattcaata aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc
     4681 aaaaaggaat acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt
     4741 gcgatgaaca aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca
     4801 aataaaatta atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg
     4861 acccggttgg ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt
     4921 tcctccttcg tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc
     4981 ttaagaggga gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca
     5041 gttatactga aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac
     5101 taaaacgaga aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg
     5161 caggcgtctt cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat
     5221 tgctagcttg gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat
     5281 tagctacagc aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa
     5341 aattgcccag ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg
     5401 ctgcattatc gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt
     5461 atcttttgtt gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga
     5521 caaaagtcgg tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg
     5581 tgattgtcat atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg
     5641 aagctggaaa agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt
     5701 ttgcttatga cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa
     5761 aagatttgcc taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga
     5821 tcaattttgt ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag
     5881 cttctgaagc atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga
     5941 tcttgatctc ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt
     6001 atgcgatggc tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga
     6061 agtttacggt tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga
     6121 gccttggttc tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt
     6181 tactgatttt tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat
     6241 ataaagtacc actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt
     6301 taggtatgac gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga
     6361 taggtatccc agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa
     6421 caacagacag aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat
     6481 aaaacaagtt cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag
     6541 taagaatgaa aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa
     6601 cagttcaatg aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa
     6661 atggcggtcg tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac
     6721 tatccaaata tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa
     6781 gaaatcgaaa ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa
     6841 attggcttgg attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc
     6901 agacagattg ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca
     6961 cttgaagaca cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg
     7021 catagtttca gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt
     7081 tcatttagcg gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat
     7141 gtaccaatcg aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt
     7201 agaggaaagc gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt
     7261 cggaatcttc cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt
     7321 ggtctaagcg aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga
     7381 cgatacaaga aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc
     7441 aatcaatgaa gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt
     7501 ttttaccgat cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc
     7561 tcaggcaaaa catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct
     7621 tggtgtcgag catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc
     7681 agacatttcc caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt
     7741 aatagctggg gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg
     7801 ttacacgaac ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga
     7861 actcgcccaa gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga
     7921 caccctctag aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag
     7981 gacaaaattt tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag
     8041 atgaacatac gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag
     8101 ccaagcatgc aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg
     8161 ctgatacgat gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg
     8221 atttgtcaac agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga
     8281 atttacctta ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg
     8341 tggatgaact agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag
     8401 gaacgaaagc ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag
     8461 cattcatcgt accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga
     8521 aactgacaag aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat
     8581 tgacaaaagc ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt
     8641 atggcaaaga tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg
     8701 atccaaaacg tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga
     8761 tgtcagaaaa tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg
     8821 catcaagtaa taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc
     8881 ttattaccgc agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt
     8941 ttaactctaa gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt
     9001 ggtcttatta cttgatgcaa gcttttgacc actattatgc cgaacaagga gatgtgacac
     9061 aaattttgtc acatctccta tttttgtttc ttgatcaaaa aattcttata cttgatactc
     9121 acactcattt tgtatggatt tagaaaagaa tatgtgtctt gacagttatt agtaagtgag
     9181 ttatactaag tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa
     9241 attcagttcg aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa
     9301 tggtcatgcc taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat
     9361 tatttattgc gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat
     9421 ttggtttctt tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt
     9481 ttgcgttgat tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac
     9541 agtttacttt tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt
     9601 ctttagcaga aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat
     9661 attattctca aggatatttt tatacagtat tcatcttgat ggggattggt gggcttatcc
     9721 acagtttagt tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg
     9781 atatccccgt atttacggca gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg
     9841 cgaagaaaac gacatattag acatgatctc tcaaaaaaaa aactctcatc tagtaatgga
     9901 taataaatcc tctaagatga gagttttttg tcctaaaaag agctgcacca acatcttctg
     9961 gcacagctct aacagggggc aatttatgga agtttgatga cttgttctgt ttctttatca
    10021 aagaagtggc ctttgctgat gttgaaggca aggtcgacta tttcgcctgg tttatggaaa
    10081 tctcttgcat ctacttttga gatgaactca gtagaaccag tttttgtgta aagcatcgtt
    10141 tcagctccaa gtaattcgga aacgacaact tctgatttca ctacggcttc aggtgaagct
    10201 tcaagggcta cttgttcact gtggatgtct tctggacgaa taccgaagat aacttctttt
    10261 ccttcataac ctttttcaac taatactttg ttttttcctt caggaatacg aagcttaagc
    10321 ccatgtccat ctgtgataac accatttttc aatgtcacat taaagaagtt catggcagga
    10381 gatccaataa agccggcaac gaacatattg acgggtgtat cataaacttc tttcggtgta
    10441 ccgatttgtt ggataaatcc gtctttcatg ataacgattc ggtcagccat agtcattgct
    10501 tctgtctgat cgtgagtaac atagatggtc gttgtttcaa gacgctggtg aagtttggca
    10561 atctcagcac gcatggctac acgtaattta gcatctaaat ttgataatgg ctcatccatt
    10621 aagaagactt ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca
    10681 ccagataaag cagctggttt tcgtttcaag tattctgtca gacctaagat ctctgctgca
    10741 ttgtccacac gttttttgat gtctgcttta tcatatttac gtaatttcaa gccaaaagcc
    10801 atattatcaa aaacagtcat atgaggatac aatgcgtagt tttggaaaac catggcaata
    10861 tcacggtctt taggtgccac gtcattcatg acttttccgc cgatatttag ttctccttca
    10921 gagatatctt ccagtcccgc gatcattcgc aaagtagttg actttccaca accggaagga
    10981 ccaacaaaaa cgataaattc acggtctttg attgatagat taaaatcagt tacagaataa
    11041 ttttctgcat tgtcatattt tttatatacg tttttcagag ccatttctac cataatacgt
    11101 tccacacttt cttttttagt tttattaact atttaaagta taaatgaaag cgttaactaa
    11161 cttcaatgtc atgttgaaca agaatttttt cttttttttg tcaatgtgct agttttaagc
    11221 tttaataaat atccataaga aataaaaact gatacactgg agtaaataag tatgttaaca
    11281 atatcaaaag gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct
    11341 atgatggtga aattagcaac agcgtataaa acaatattgg aaaaacatga actttttgct
    11401 accgggacga ctgggcagag gatcacggaa gcaaccggct tgccgatcca tcggtttaaa
    11461 tccggaccgt taggcgggga tcagcaaatt ggagccatga tttcggagga tcagttagat
    11521 ttggtgattt ttcttcgaga cccgctggct gcacagcccc atgaaccaga tgtaacagca
    11581 cttatccgtt tatctgatgt ctatgagatt ccgttagcta cgaatatcgg aacagctgag
    11641 gtgctactaa gaggactgga agccggcttc ctagattttc gaagaatcgt ccatgaaata
    11701 gacaagcatc cactttcatt ttgatggata agcgtatgga aaaataacgt ttaatgttcg
    11761 cctatatgta gctataaaag ctaaacaaac tgataatatg cgatataata cgaacgtatt
    11821 ggataatata aacaataata ttagtttttc gtatttgcag taaatgtgaa aatatgctat
    11881 agtttctgtg ggaaaagatc ccaaaaataa tacagagtag aaaataaagc gttaagtgct
    11941 aagaggatgg gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt tattttcgca
    12001 ttcgctgcat gatacgatgt agcagctctt aaaggagatg ctaaaaatga agaaacaccg
    12061 gttagacgca cgtatgattg ctattatggg cttattgatt gcattgatgg tcactttgtc
    12121 ccgactggta gcaatcgaga ccccatttat taaaatcagt gtcacattta ttccacaagt
    12181 gatcatgggc attctttttg gtcctttttg gtcagggata ggcgcagttt tagctgattt
    12241 agtaggaatg gccctcttct cgaaatctgc attctttatt ggatttacat tgaatgcatt
    12301 tattgaagga gcaatctacg gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc
    12361 cattctggct acgcttagtg tcacgctgat catcaactta tttttgacac cattatggct
    12421 tgcgttgatg taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa
    12481 aacagtaata tggttaccga ttcaatcaat tgccatttat tacgtaggac gtagtattcc
    12541 ttacaaaaaa attttaagaa gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc
    12601 catcccagaa gactgcttgt ttttattgtg ctgtgaattg tgcttgatag agccgtcggt
    12661 aataaccatc tttttgaagc agctcatcat gggtgcccat ttcgacaata gctccctgat
    12721 ccatcactaa tatctggtcc gcatttttga ttgtggacag gcgatgggca atcacaaaac
    12781 tggtttttcc ttccatcatt gctaagaagg cttgttggat ttttttctct gtcaatgtat
    12841 cgactgagct ggtagcttca tctaagatca acataggggg attactgatc atcgttcgag
    12901 caatcgtcaa cagttggcgt tgtccatctg atatctttaa cccttgaccg ccaatcttag
    12961 tatctaattt ttccggcaaa cgttctacaa attcatacat ataagaggct tttagggcat
    13021 cattgatttc tgcgtcactt gcttccggat tcccaaaacg aagattttct cgcaatgtac
    13081 tgtcaaatag ccaagtatct tgtaacacca ttccaaaact tttacgtaat gtatcacgtt
    13141 ggatagagcg gatgtcatgg tcatcgactg taattgttcc ttttgttgtg tcataaaaac
    13201 gcatcagcaa attaacaagt gtggattttc cggcccctgt ttttcctact atagcaatgg
    13261 tttgacctgg ataagcagta aaattgaaat cttcgatcaa acgctgcttg gggtcataag
    13321 aaaaagaaac atgatcaaaa gagacttttc ctttgatatt tttgggttcc aaggcatagg
    13381 catctggcaa atcaggcttt tcttctggct gatcgatcaa atcaaatgct cgttctagac
    13441 cagaaaaagc ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa
    13501 actgacttga gtagatcgta aagctggaaa tcacaccaac agtgattgac gaaccactct
    13561 tcaatgccaa taggccgcca actaaaccaa tcgataaata agcaaggtga tctacaaaac
    13621 gagaagacgg gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt tcatatagat
    13681 cagcatttag cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa gctttcacga
    13741 ttttctggtt accgattcgt tcggtcacaa agcccgagat atccccaata atcctttgct
    13801 gtctatcgaa atttgtttgt gaagcacgag caacgatcca actcacaagg aaaataattg
    13861 gtgtagaaca aagaacgacc aatgtcagaa gtggacttag acgcatcata aaaataaacg
    13921 aaagaatgat gattgccact ccagaaaata attgattgaa ggccgcagaa attgcaatgg
    13981 aaatattgtc catatcattt gtaaatcgac tgacgatatt tccgtgggag ttttgatcat
    14041 aataacgaag tggcaatcga ttcagatgag caaatgcatc ttttcttaat tttgcaacgg
    14101 ataagtaagc taaccgattg cttaacactt ggatgagcca ttgactgact acgttgatta
    14161 aaacgatacc accgaataga ataagtaaat ggaacagtcg attgaagcgg acctgtcctt
    14221 ctccaatcat ttgatcgata ctttgaccaa tttgtaacgt catcacaact gttgcaatcc
    14281 cattgatgat tcctagtatc acagctagcc aaatgtcttt tgcataggca gtaaggtaag
    14341 gaatgaatcg gcggagcgtc tgcaaggata atttttttac tttcataact attcttcctc
    14401 ctgtgatgcg acgatttcct gatattctgc tgattgagca agtaattctt catgggtacc
    14461 ctgagcagca atcttacctt gatccatcac taagatatga tgtgcttctt gaatagagcg
    14521 gacacgttgg gagataataa tcactgtact gccaagataa gtctgtagcg ccttacgcaa
    14581 atccaagtct gtttgataat ccaacgcact taaagagtca tctaaaatca gtaatgctgg
    14641 cttggcaatc aaagctcttg caatcgtcag tcgctgtttt tgtccgccag agaaattttt
    14701 cccattttcc atgacttttg tatctaaacc atttggaagc tgacggacaa attcttctgc
    14761 ctgagcgatt ttcagagctt cccaacattc tttttctgtg gcgtctttct taccccattg
    14821 aaggttttca cgaatagtcc cggtaaatag tacagaagtt tggggaacaa gagcaatttg
    14881 ctgacggaga tgatccagct gccaagaacg aacatccatt ccgtccatta taactgtacc
    14941 ggaactgaca tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc cgcttccggt
    15001 tggaccaatg attccaagga ctgtatttcg cttcagctga aatgtaatat tttggagaga
    15061 aagaccagaa tcaggagtat aacggaagtc aacatggtca aaagcgataa ttgcttcaga
    15121 agaaaaatcg ggagtagtac tgacctcagc ttcaagaatc gaattttctg tgtctagcac
    15181 ctctttcacc cgatttcctg aagcttcggc acgtgtgaag atcacgacga ggtttgaaac
    15241 aacgatcagt gccaaaagca tttgattcat ataattgatc aaagcaagaa cttgtccttg
    15301 ttgcaagcta ccaatattta ctttgatccc gccaactgtc agcaaacaga tgatcccgac
    15361 attcatgatc aaggttgtag caggtgataa tagggcagag atattggaaa cacgtaaata
    15421 attgttcgct agatcatttg tgcttttatc gaacttctct gtttcttttt ttgtctgcgc
    15481 aaatgcgcgg atgacacgaa caccgctaag attctgacta acgtgttcat tgagctgatc
    15541 tagtttttct tgcacttttt ggtagagagg aacggttttt ttgatgataa aaaataaaat
    15601 gattgagaaa ataggcaata atgctaagaa aaacagacct acttcccagt cgatcacaaa
    15661 agccatgacc actgacccga tacttaaaaa cggtgctctg atcaataaac gaatgaccat
    15721 tgctaaagct agctgaagct gattgatgtc gtttgtcatt cgggtgatca aggtatctgt
    15781 tccaaaatga ttcaactcgt tgtgagagaa agtattgatt tttttgatta gctgattacg
    15841 taactctgta ccaaatcctt gtgaagctac tgaagcatag tattggcaaa tgatagcaca
    15901 aatcagacca atcacagaca tagcgaccat ccataagccc atttcaatga tttttcctcg
    15961 gtcattcatt tttagtccat tatcgatcaa ggaagccatc attaaaggca ggacaagttc
    16021 gaagacagct tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac gatagtcttt
    16081 tgcataacgt aataactgaa tcatatgatt cctcctatta tttatttgtc cacaattgaa
    16141 aaaacaagta ctaccacatt gtagcttagg a
//
LOCUS       DAYYHX010000049        15673 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000049 DAYYHX010000000
VERSION     DAYYHX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.049"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..1071)
                     /locus_tag="ACHA6Y_001166"
     CDS             complement(85..1071)
                     /locus_tag="ACHA6Y_001166"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8987276.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1094..1660)
                     /locus_tag="ACHA6Y_001167"
     CDS             complement(1094..1660)
                     /locus_tag="ACHA6Y_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM8987277.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(1674..2090)
                     /locus_tag="ACHA6Y_001168"
     CDS             complement(1674..2090)
                     /locus_tag="ACHA6Y_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8987278.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(2547..4481)
                     /locus_tag="ACHA6Y_001169"
     CDS             complement(2547..4481)
                     /locus_tag="ACHA6Y_001169"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8987279.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            4783..5718
                     /gene="pplA"
                     /locus_tag="ACHA6Y_001170"
     CDS             4783..5718
                     /gene="pplA"
                     /locus_tag="ACHA6Y_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM8987280.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            5808..6911
                     /locus_tag="ACHA6Y_001171"
     CDS             5808..6911
                     /locus_tag="ACHA6Y_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8987281.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            6924..7877
                     /gene="menA"
                     /locus_tag="ACHA6Y_001172"
     CDS             6924..7877
                     /gene="menA"
                     /locus_tag="ACHA6Y_001172"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8987282.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(8691..10037)
                     /gene="gor"
                     /locus_tag="ACHA6Y_001173"
     CDS             complement(8691..10037)
                     /gene="gor"
                     /locus_tag="ACHA6Y_001173"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM8987283.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10171..11424)
                     /locus_tag="ACHA6Y_001174"
     CDS             complement(10171..11424)
                     /locus_tag="ACHA6Y_001174"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM8987284.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11430..12353)
                     /locus_tag="ACHA6Y_001175"
     CDS             complement(11430..12353)
                     /locus_tag="ACHA6Y_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM8987285.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(12574..15066)
                     /locus_tag="ACHA6Y_001176"
     CDS             complement(12574..15066)
                     /locus_tag="ACHA6Y_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM8987286.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15073..15540)
                     /locus_tag="ACHA6Y_001177"
     CDS             complement(15073..15540)
                     /locus_tag="ACHA6Y_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM8987287.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4564 a         3272 c         2791 g         5046 t
ORIGIN      
        1 aatacaagaa ctacaattca atttgtcaaa aaacacgtag atttccttct gagaaattta
       61 tttacgtatc gaacctttcc ctattcagtc tttgcgatcc aaaatcagtt gggtaaggga
      121 aaagagattc tcttttgttc gaagagaggt ggcaggtaat ttctttattt ccttcaaagc
      181 cttttctgtg taagaggccg ctaacttcct agcttcttcc acgcctcctg tatgttcaac
      241 gattttgcgt acggcgtcga catcttcttc tgtcattgcc gctttcttct ctaaataagg
      301 tagtagcttt ggtttgtttt tttgcaaaga gtagatcaac ggtaaagaat agaccccttg
      361 acgcatgtct tctagtacag gtttgccgat ttcctggctc tcttttgtat aatctaaaat
      421 atcatcgata atctgaaagg ccatcccgat agaaagtccg atatctcctg ctttttttgc
      481 gaagcgctcg cttgttccac tttcaaaagc gccaacagaa caacttaatt ggaacagttc
      541 agctgtctta cctgaaatat tttccaaata ttcttttaca gaaagttcat aattatagcg
      601 atcatccatc tgccctaatt cgccattcaa tactttttcc atactgcgag aattcaattg
      661 gagacttttc aaggaagtgg agtaatcaga caatagttta aaacagcaga caaataaata
      721 gtctcctgca tagacagaaa cgacattacc aaactgacta cggatattag gctgaccacg
      781 tcgcagatcc gcttcatcaa cgatatcatc atggatcagt gtggccgtat ggagcatttc
      841 gatagaagct gccaaagcga tggctttgtc tcgatcttgc tctggtccaa attgtgagaa
      901 caatagctga taggcaggac gcaacagctt tccgccggca tggatcgttt ctttgattgc
      961 ttgttcgaca ggtttgtttt tcaggcggac agtttcttcc atccgtttta gtgtttctgt
     1021 caattcttta cttaaagcag gatagttttc ccacattgga tgtagcttca taaaaaaact
     1081 ccttgtgtga cgattatttt ttggtaaaca ttgggctcca tttttgtttg gttccctgct
     1141 cataatcttt ttggaatcgt tgaagtgtct gcacatgtcg taaaagataa ttcgctgcga
     1201 tcccgattgc aatccctgat aaaatcccta aaactgaaag gactggcaag tacaacatga
     1261 ctgtccatga ttgtgcaatg aaagaagcca tcaaaagctg tccgacatta tgcatgaaac
     1321 cgccggccgc actaattccg atgatactga cacgttttgg tcctaattgc ttgaccaata
     1381 acattccaaa ataactaagc aaagcaccac tcatactata aaggaatgtg gaaatcgttc
     1441 cgcctagcaa agtagtcagg aatagtcgta gccaaagtag taagaagctg tcttgttttt
     1501 tcatggtaaa aatggcaacg atcgtgatta gattggctaa gcctagtttt gcaccaggcg
     1561 caaaagaaaa aggataaggg atcatatttt ccaataaacc aatgatcact ccttgtgcaa
     1621 caagtagcga aatataaatc attttttgta atttactcat ggtgtgtaaa ggtctaataa
     1681 atcagactgt cttcgtcacc tccatctgtt gaacggactt cgatcactaa tttatgtggc
     1741 aaacagacga tggtttcgcc atttttcgtt gcccagcctc gacgtacgca gatctggtca
     1801 tcgcaatctg cttcttttat ccgtactcga tcgccatcta tctcgatcaa gttataatct
     1861 ccgtgttcat ctttgtaagt atatgtgttg ctctcttctc cagccatcaa agggaatgtt
     1921 ttgatttctg tgccatccac acgcagtacc gcttcttttt ctgctgttgc ggtgttttgc
     1981 tgaagaccaa aaatcatgac tggtaaaaaa gaaagaacag tcaaaagcaa aataataaaa
     2041 acatcccaag gtttgagcag acttttttca gcaaattctt taaggctcat ttttgttctc
     2101 ctttttccag attaactcac ccaattttag cataaaaaca agaggttgtg acacctgtgt
     2161 cttgctttga gtaataagaa cgaagaatag aaaacagctt tccatgtttt ctaattcttt
     2221 gggcttatta ccgagaagca gcaggtggaa caccgtttat aacaagaggt tgtgacagaa
     2281 gtgggcaact tcaagaaata agaaggaatt tacgaaaatt acttttcaag tttttgtgaa
     2341 atttcggctt atttttcgaa gaagctactt ctggaacacc gtttataaca agaggttgta
     2401 acagaggttg tgacacctgt gtcttgctca tggaggctta tctcgggcat cttattcctt
     2461 taattttaca taaaaaggag gctgtgacgg tttgtctcag cccctttcta ttgaaatctt
     2521 gcattcaaat aacgttcttt atctatttat ttcttgccgt actgggaact tggtctaccg
     2581 taccaccaat tacctagtgt acgttgtagc catggaatta cccagcgatc caacccaatc
     2641 gcacgtccgg aaccgttcat caatgcgaaa gcaacaaaaa tgaaccaaat gtttacccag
     2701 taaaacatgc ctgataagca gaacgtgatc accaatacaa tcgttgtcgc actgcatagc
     2761 catgtgaata atccagcaat caatgctaat gccaaggcaa tttcaaagaa taccatgaat
     2821 ttttggaaga acattgctac atcattgttt ggcatgatca cttgcatgat ctttgtgaac
     2881 cagtctggtg acttctcaat cacagccatt ggttcttcac cgtatgcata actcaaacca
     2941 aaatgtgcag cttgagtcgc tgcttctccg gcacttgccc ctgctccgct ggcagctgtg
     3001 gttgcatcac cagcagcaga agcaccactg gcaacatctg ttacattgta ttcccatgtc
     3061 caagggaaca cactgtttcc agtgaaccat gatccttcac caaaccaagt acttggtttg
     3121 aacacttcac cattcccaat gatttttttg gaagcttcaa cgaaccaaac taaaccatag
     3181 aaaatacgaa gtggcacact ccataagaca ttgccgtaac gagaagaatg tcctcgagtc
     3241 acgttgcgat catctttgat atggaaaaac tcatgcatta aatattggaa catataataa
     3301 ccagaacgaa tatcaaagaa gtatttcaaa ttcacgatat gtttcattac gatagctaaa
     3361 aatccgctta aatggatctt atcaaatagg ttagctaccc cccatttggc accaatagaa
     3421 accataaatc cttgatagtt tcctttaaat ttatgctttt ctccgccttt gatcgaagcc
     3481 acgatattcg ctgcggctgt gtgtcctgtt tgttcagcag cttgaacgat ctgcggtgtc
     3541 ggtgttcctg ggaattcttc ataaaagact aaatcaccaa tgatatagat gtttttatct
     3601 tcgtagcctt ttgcctgcat atattcattg gcgatcaacc gctgtccgcg tgctgattct
     3661 aatccaaagt cagcagcgtc agaagttcct ttgacaccag ctgtccaaat cagtgtgtga
     3721 gtcggaatgt tgcgtccgtc tttcaatttg atatgatcag cagccacttc tacgattggt
     3781 gcattcaata ataattccac gttgtgctta cgcaaatagc gttcagcttt tgcagcatca
     3841 tttcgatcca acatattcaa gatcgtcggc attgcctcta cgaccatcag tgtgatttcg
     3901 tttggatcta atttataatc tttggctagg cgatttttcc agtcgatcag ttccccaatc
     3961 atttcaatac cggtaaatcc agaaccacag acaacgaaag tcagcatggc tttacgcagt
     4021 tcatcatctg gttccaatgc agcttttgct actgtcttct caatatgttc ccgaatcctt
     4081 acagaatctt caaatgacca aagggtgaat ccgtgtgctt tgacaccagg tgtaccaaag
     4141 tcattcggct ctccgcccat tccaaggatc agctgatcaa aaggatagga acctaatttg
     4201 gtcttgacga tttttcgttc tttatcgata ccgattacag tgtctgtgac aagttgtaca
     4261 ttcttttgac gagcaaacaa atgctgcaga tcgtattgga ttgcttctgg ttctacacgt
     4321 ccgccagcta cttcatgcaa ctcggtcatc atagtatggt acgaatgacg atcgatcaga
     4381 gtaattgcta catcttcttt tttcagtttc tttgccaagt attttgtagc tgaaacaccg
     4441 gcatatcctg ctcctacaac gacaatattt tgctttgcca ttgatcatcc gctccttata
     4501 gactatctat ataatgaata tatgataatt cttttagttt gatacttatt acacaatctc
     4561 catatattat atagattttc tgttcttttg tctaactcct tttggatttt tttcaaattt
     4621 taaaataaag cgttttttca cgaaatttgc tgaaagaaaa cgtttgaaat attacatggg
     4681 ttatttatca aaaatagttg acttataggc taaaaagatt agaatgtata atgaatcaca
     4741 aagaaaaatt gtggactaga aatggaatgg tggggtatta aaatgaagaa aaaacgtttt
     4801 gtaactggat ttgccgtatt agcattttca gctctagtgt taggcgcttg cggcgcagat
     4861 gacaacggaa gctcaaactc ttcttctgaa tcatctacag cacaatcttc gactgcaaaa
     4921 agcacaacag aatcatctgc aaaagttgta gctggcggag atttgcaaga cggcacatat
     4981 aaattagaag aaaaaaatta ttcaaatggt taccgtgcag tctttgaaat ggttgtaaaa
     5041 gacggcaaaa tcactgaatc aaaatatgat aacgtcaatg aaaacggcga atcaaaaaca
     5101 aaagatgcag aatacaacaa aaacatggaa gcaaaatctg gcacaaaccc agaaaaatac
     5161 attcctgagt tgaatgacca attcttaaaa gcacaaagcg caagcggtgt ggaagtcgta
     5221 actggagcta ctcactcatc tgaaagcttc caaaactacg cacaacaatt gatccaagct
     5281 gctcaagctg gtaacacaga tacaatcgaa atcgacaatg gtgctgattt gaaagacggc
     5341 acttacaagc ttgaagaaaa gaactactct aacggctacc gtgtgcaatt cgaaatgaca
     5401 gttgctggcg gcaaagtaac tgaatcaaac tttgattata tcgacaaaga cggtaaatca
     5461 aaacaagatg atacagaata taatgaaaat atgaaagcaa aaagcggtac agaaccaaaa
     5521 acttacatcc ctactctgaa cgacgaattc gtcaaagcaa tgggcgaaga agatggctca
     5581 cctgctaatg tagaagttgt tacaggtgct acacacagct cacattcatt catcatgtac
     5641 gcacaacaat tagtgaatgc agctgaaaaa ggcgacactc aaacaatcga agtagataat
     5701 atcgtaacag aaaaataatc acataatcat actaataaaa gttggagggt gggacgatag
     5761 ccagattagg cagtcgtctc acctttcaaa atgaagagag gaaaaagatg cggaaaaaac
     5821 tactattttc cttaatcaca ctgggcatct cttttatggt gctaactggc tgcagcaact
     5881 cagcaaataa aacaaccact gaaagttcag cagctaccaa gatattgaaa gatccatatt
     5941 ctgatgaaca gttcttgtta gggacttacg ttcgtatccg tgtgtatgat gaaggcaaag
     6001 aatcagcatt gaaacctgcc ttcgatcgag taaaagaact tggtgataaa atcacgatca
     6061 atcaaaaagg ttctgagatc gatgaagtga atgagcaagc tgggatcaaa ccagtaaaag
     6121 tttctgatga cgtctacacg ttagtcaagc gggcctacga atatagccaa gattctcaag
     6181 gcggattcga tatggcgatt ggtgcaatca cacaattatg gcgaatcggt tttgatgacg
     6241 ccagaaaacc ttcccaagaa gaaatcgatc aggcattgaa attagtggat tatcataaga
     6301 tcgaactaaa tgacaaagaa aaaactgttt atctaaaaga aaaaggaatg atcatcgatt
     6361 taggtgcaat cgccaaaggt tacattacgg acgaagtagt gaaagtcttg aaaaaacaag
     6421 gagtgaccac tgccatcgtt gacttgggcg gtaatgtcta tgtcttaggc cacagcccaa
     6481 gaggcgaaaa ccaagactgg acagtcggca tccaagatcc gaacatggct cgcggatctg
     6541 ttttaggttc tattaaagaa cggaacaaaa cattagttac ctctggaatt tacgagcgtt
     6601 atttagaagt agatggcaaa aaataccatc atttgtttga tccaaaaacg ggttatccgt
     6661 tcgacaatga tatcgcaagt gtcacgatca taacagataa atcaattgat ggtgacgggt
     6721 tgtctacagc agtcttttca atgggtgtga aaaaaggact ggaatacgtc gaaagcgaat
     6781 tgaataacgg gacggaagcg atcttcgtga caaaagacga taaagtatat gtgaccgatc
     6841 caatcaaaga caccttcaaa ttatcggagg actcccatta cacgatgggt gaccgttctg
     6901 aactaaaata aggagtacga accatgacac taaaaatctt tttagaagta gttgaaatac
     6961 gaacaaaggt ggctagtgta tttccttttg cggtaggggt attattctct atcgcttttt
     7021 ttcatgagat acattggcta aacacttttt tattttttat tggaatgcta gtttttgatt
     7081 tagcgactac cgctatcaat aattacatgg attatgaaaa ggctcattct gaagtatata
     7141 agtatgaaga aaatgtcatt ggccgcagaa atctatctcc tgtattgatt cgaaacatga
     7201 ttttagctat gattgcattt gtttttgtta tcggcgttat tctaacgatc aatacgggtt
     7261 ggcttttact tttgatggga tttatctgtt gttttattgg agtattctat acattcggac
     7321 cgattccttt atctcgaatg cctttagggg aaattttcag tggggtgact atgggccttg
     7381 gaatttttgc catggtcgtt tatgtcaata cattcggttc agatgtctat gatctgacag
     7441 tcaacttttc atctggacaa tttgctttgg taggaaatct ttgggctata gcagcaatcg
     7501 tattagcttc cttacccttg gtgtttacga ttgccaatat catgttggct aataatttgc
     7561 gggatcttga ccgtgatatt gaaaatcatc gctatacact agttttttat atcggacgtc
     7621 ctgcgggaac agtcttattc cagctattga tgtatgcttg ctatttcgtg attttaatgg
     7681 ggttgttgag cggtatctat gaatggccga tcttggtcac cttcttgacc ttgccaattg
     7741 tgaagaagca tttggatcaa ttcaagcaaa cgttacccca gcctatcagt ttcagctatt
     7801 ccatcaaaaa tatggtctta ttcaatgcca gttatgtatt ggggctatta ctaacaatca
     7861 ttatgcaagc catttaacag catgaagagg ttgtgaaaaa actgccctgc atcaagtaat
     7921 aagaacagcc aaataaaaat agctccttat atttttcgtt tggttgggct tattaccgca
     7981 gatgcaagct tttgaacacc gtttatcagg aggttgtgaa aaagtcgctt agtttcgagt
     8041 attaagaaaa attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc
     8101 gagaaactgg cttttgaaca ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag
     8161 tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg agctaatacc
     8221 gaaggacagc tgattgaaca ctatttgttc ggaaaaagtg actaactatg acacaatttt
     8281 tttcacagtc ccttttttca cttgatttga ttcgtaccag cgaacttgac gagaactttc
     8341 ttggtttggt aactatatgg tattctttta acatcctcgt ttgcttcttc taatttattt
     8401 atacaaagtc acactttttt aaacatctgt tcttcgatac atttctattt cttttaaaca
     8461 tcgataccaa tattttgaac ttaagagtgc ataccaaatg gattcattta ctcttcgtca
     8521 gttacgaaga taatcaaaat ctccaacttc cctacttatt tcgttttgag aattatcgaa
     8581 gcaatcttac ataaataact aaaaaacaaa aaaccagaaa atcagtatac tgattttctg
     8641 gttttacatg gtttgttcaa taaaaagctg ttggaactgt taatattaaa ttatcgcatc
     8701 gttacgaatt cttccgctcc tgttggatgg atggctactg tattgtcaaa gtcttcttta
     8761 gttgcaccca tcttgatagc cacggcaaaa ccttgcagca tctcatcgac accaacacca
     8821 atagcatgta agccgacgat tctttcttct tttccgacac agatcaattt catttcgcat
     8881 ttttggcgat aatcatttaa tgcaaagtac attggggtaa agcgtgaacg ataaactttc
     8941 aattcatttt cgccatattt ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt
     9001 ggtggatgcg taaagacgac tgtcggaaca agtttatagt caagataaga atctttcttt
     9061 ccattaaata aacgttctga taaacgtcta ccagctgcga ttgcaacagg agtcagatct
     9121 aatttaccga tcacatcacc aacagcaaaa atatgttcat ccgttgtatt ttggtattta
     9181 tctactttga taaacccttt tttgtccaat tccacatttg tattttccag acctaatttt
     9241 tcggtattcg gtacacgtcc accggcaaaa agtacacaat caccagttaa ggtttcacca
     9301 ttttcaaata gcaccgtata ttcgtcattg tttttagtaa tttctttagc aatatgatgt
     9361 gcgtaagttt ttattccagc ttcttggtac atttggatca aattgtccga aagcatttta
     9421 tcaaacgttc taagtggtcg ttcttttcga tatgcccaca tgacttcact acctaaacca
     9481 cgaaatacac ctgaaagttc agccgcaatg tatccagcac ctaaaacgat tgctctctta
     9541 ggtaactctg ttaattcaaa aaatccattt gaatcaattg cgtattctcc accaggaatg
     9601 gtcattttac ttgcacggcc tccagtagcg atcaagattt taggagcacg ataggtttca
     9661 ccgtttactt cgatcgtttg actatcgata aaagtagcgt aaccttttat agcctcgaca
     9721 tgattgctat ctaatcctct ttgataagca ccgtgtagaa aatcaatata tttttctcgt
     9781 ctttctacta gaccggcgaa atcaacattt tttaaatctg cttggatacc gtaacttgag
     9841 gcatctcgtt caattgtttc caaaatcgta cttgcttgcc acataacttt tttaggtaca
     9901 caaccgacgt tgacacatgt tccgcccaat tcattcgctt caatcagcaa aacttttgcg
     9961 ccatgcatcc ctgctctgtt tgctgaagca ataccgccag agccgccacc aattacgatg
    10021 taatcatata tttgcatttt gttcctcctt attgaagaga tcaatcaaaa cctttattta
    10081 acgatccttt ttctacagaa aacaagtgta acacttttct tgaattttgt atgcgaagat
    10141 gcttaaaaca gggatatatc agctttttcc ttatttacgt tttcggatca tatctccagg
    10201 tttcaccggc tgtgcgactg gcatagttaa aatcatcatt ggatttggtg ctcggtcaat
    10261 ggattcgcct tcttcattat gcataacttc aacggtttga ctgaaatgac tgaatcctgg
    10321 accataaaat tcgatttcat ctcctacaga aaacaagttt ctctgacgta tcgtagcaat
    10381 ctgtgttgcc tcatcataag ataatacttc accgacaaat ttatatactg ggattttacg
    10441 acgttcaccg aataattgtt cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa
    10501 ttctcgttgt gctactttcc ataactcgtc gatccattct tgcttgcaga catagttttc
    10561 aggatcttcc atataactgt ctacagccgc tttatagaca tttgacaccg tagatacata
    10621 atggatcgat ttcattcttc cttcgatctt gaagctgtct acgccgtttt caatcaattc
    10681 tggaatatgt tcgatcattg acatatccac tgcactcatc gagaattctt cttccacttc
    10741 gcctgtatca gttaaactgt ttcgttcttg accaaaaggc atatcgaaca atccatactt
    10801 ccagcgacaa gattgagaac atccgccacg gtttgcatcc cgcatcgaca tatggttgga
    10861 caatgtgcat cgaccagaat aagagataca catggcacca tggataaatg cttcgatttc
    10921 ggtatccgta tttttacgaa tctctgccac ttcctccatt gaaacttcac gagctaaaac
    10981 gacacgttcc aacccttctt ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc
    11041 ttgagtggat aaatggatcg gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc
    11101 tggatcagaa acaatcactg cagaaatacc gacatcacgc aaactgcgga aaaattcccc
    11161 tgctccatct tcatttcctt catgggtgac catattagca gcaacgtata ccttcgcatc
    11221 atgttctttt gcaaaagcaa ctccttcagc catttcttca taagtaaaat tacctgcacg
    11281 gcttcttaaa ccatatgcat tcccgccaat atatactgca tctgctccat aatggatcgc
    11341 ggtttttaac ttttccagtg ttcctgctgg cgctaggatt tctggtcgtt tcaattttct
    11401 ttttactgtc atttctttca tcatactcct tatttgatag cagaaggatc aatataaaag
    11461 aatcctgtat ctaattcacg gttttcagga tgcaatttac tgatttcgct agtcattttt
    11521 tccgcttttt cttgatccca gctaccttct aaaatttgtt tttttgcttc ggcgaatagt
    11581 cgagtgatgt ttacgaattt ttcgcctgga gtaaataatc cgtccaattt ccatgtacag
    11641 taatgatgct gataaagttc attcaattca ttcgagagat tcagatcatt gctggcaaaa
    11701 atatgtgtgc catgactatc ttcataaata gaataatgtg tctcttcttt tttaggttcc
    11761 gagataaaaa gacctctttc acgatctttt tgttcgtctt gtttcgtata gttatagtaa
    11821 ttctgtagca gtggacgttt ggattgatgg atgcatgtag caccatacac aagggtttct
    11881 acaggaatat cgagtttttc ttccatcgcc ttcatctctt caaaaggaac ttcacgagca
    11941 agtactgctc caactgcgcc ttttttcgcc cagaaattga tttgacgtgc gctcgttact
    12001 aatgtttcag catcataaat atacggtata cgcaattcag gatttttttt catcgtataa
    12061 ataatacctg tatcaccaac tgtgatcaca tctacgttta tttcttttaa aaaagccaaa
    12121 tattcaggta catttttcat tttttcagga tgaatgatcc catttacagc aatattcgct
    12181 tttttaccat acttgtgtgc taattctacc aattgtcgct gttcttctcg tgtaaatgaa
    12241 gcaggcagac gcaatccaaa ttgctcttca ccgaaatata gtgtatctgc acctgcagca
    12301 agcaactgtt ctgcttgttc aatagtttct actgtggtta tgagttcgat cataattttc
    12361 tcctcctaca acttcataat agtagcatag ctctaaaaac tttccaatac ttcttctatt
    12421 taaagtcgga tttttgaaaa ttatttcaca aaaaatcaaa ataaagttcg atatattctg
    12481 tccaaatata aaaagagatt gtgacaaaaa tcttgtcaca acctcttaat caagtcttac
    12541 tattataaaa attcagctac acaggccaag taactatagg gaacctactg tttccttttt
    12601 aggtccgcga acattcaatg tgatcttacc ttttgaagca cctactgtta cgtggtcacc
    12661 taaacgaatt tctccgccaa gcaaagcctc gctcaatcga tcttcaattt ctttttgaag
    12721 tgcgcgacgg attggacgtg caccatattc aggatcaaat ccagctttcc cgatcacatc
    12781 gattgcagat ggtgtgattt tcacttgaat atcttgttct ttcaatcgtt tgataatcgc
    12841 tttgctcatg atttggacga ttgtatggat ttcatcttta ctcaatgaat ggaagacgac
    12901 agtctcatcg attcgattca agaattcagg tcggaaagct ttctttaact cttctaagat
    12961 acgtttttgc atggctttgt gatctttagt aacatcttgg acattgaatc cgacattttt
    13021 ttcttctcga atttgtgtcg ctccgatatt tgaagtcata atcatgattg tatttctaaa
    13081 gtccactttt ctacctttag aatctgttaa atgaccatca tccaatactt gcagaagtaa
    13141 attgaaaacg tctggatgtg ctttctccac ttcatctaag agaatcactg aatatggttt
    13201 ttgacgaatt ttttctgtca actgtccacc ttcatcatac cctacgtatc ctggaggaga
    13261 cccgattaat cggcttgtac tgtatttttc cataaattct gacatatcaa cacggatcag
    13321 ggcatcttca cttccaaaca tgacttctga aagtgctttg gccaattctg tttttccgac
    13381 accagttggt ccaaggaaca tgaatgaacc aattggacga tcaggatctt tcaatccgct
    13441 tcgtgcacgg cggatcgcac gtgaaactgc tttgacagcc tcatcttgac caacgacacg
    13501 ttcatgcaga agtccttcca gttctaacaa gcgttcgctt tcttttttct ccaactgctg
    13561 tagagggaca ccagtccact ccgatacaac tgtagcaaca tcttcttctg ttacacgatc
    13621 agaataacct tgctcctgtt ttgcttcaat tgcagaaact ttttgcagct ttttacgtag
    13681 tgctttttct tgtctgcgta attgagcagc attttcaaag tcttgttttt ggatcgctgc
    13741 ttccttttct tgtactagtt catcgatttc tagtttgatc acagttgatt ttgttaagtg
    13801 atcggtttga tccaaacgga ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc
    13861 gggtagttga cgagaagtga tataacgaac agaaagattg actgctgctc gtacagcttc
    13921 atctgtgatc tccactccat ggtgctcttc atatcttgta cgtaatcctt gaagaatcac
    13981 ttctgcttct tcaggagttg gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc
    14041 agaatctttt tcgatatatt tttgatattc atctagggtc gttgctccga tcgtttgtaa
    14101 ttctcctcga gctaacgccg gtttcaagat attcgaagca tcaattgctc cttctgctcc
    14161 acctgcacca attaaggtat gcaattcatc aatgaataag attacttgac catcattata
    14221 gatttcgtcg atgacctttt tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc
    14281 aaccaaagat cccatatcaa gcatcatcag gcgcttttct tgcatgtctt ctggcacttc
    14341 acctaaaatg attttttgag ctaacccttc agcaatggct gttttgccta caccaggttc
    14401 acccaccagt acagggttat tctttgtccg tcggctaaga atctggataa gccgtttcac
    14461 ttctctcgaa cgaccaacaa ctgggtccag cttgttttct tttgctaatt tcgtcaaatc
    14521 acgtgctaat gaatctagcg taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt
    14581 tacatttcta cgacgattca ttccttttcc agcagaatca ccagctagcc ccattttttg
    14641 tttcagaact tgacgggctt ttgaaaggct gatacctaag ttgatcatga ttcgagtagc
    14701 catgatactt tcatctttta ataagctcat caaaatatgt tctgttccaa ctttttgaga
    14761 accagttttc ttcacttcgc tgctagctaa agcaaaaact tgtttcattc ttggtgaata
    14821 tggaagaaag actcctttag ggtaagaacg gataccacca taaccaatca gatgttcaat
    14881 ttcttcataa atttccgttt cattcaaatc catttcacgt aatacttttc cagcaattcc
    14941 attttgctcg accactaagg ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa
    15001 ccgttttgct tcttcttgtg caatttctaa tactgcttgg gctctttcag taaaaagttc
    15061 ttccatctaa tctcacttcc catcttcata acttaaacgt tccagaaatg ctcggagaat
    15121 ccgagcacgt gtatgatctt catattcatt gaaattcaat gtgttttttg caattgcact
    15181 caacatcaag tttccttctt tttttgtaat tatttggtct tcataaagtt tttgaataat
    15241 agcaaaagca tttttctcac tgattgtatc atcaaataac tgatttatct gctcaagtaa
    15301 ttgtttttta tctgaaatcc ttacttttgc aattcgtata taaccaccac caccacgttt
    15361 gctttctact agataaccac gttggatggt aaaacgggta ttaatcacat agttgatttg
    15421 tgaaggaaca cagttaaaga ggtctgccat ttcaatcctt cgaatctcga tcatttcact
    15481 attttccaga attttcttta tatatgattc aattaaatcc gaggtattct gatgtgccat
    15541 cttattcatt ccctttgact aatattgacc ttaattataa cgaatttgat aatagaaaga
    15601 aagaagaacg ctttttgtta aataaatcct ttagaatcaa caaaaaaaaa tacggaatat
    15661 actattccgt atc
//
LOCUS       DAYYHX010000050        15550 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000050 DAYYHX010000000
VERSION     DAYYHX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.050"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..408)
                     /locus_tag="ACHA6Y_001178"
     CDS             complement(<1..408)
                     /locus_tag="ACHA6Y_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987288.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLE"
     gene            complement(703..1038)
                     /locus_tag="ACHA6Y_001179"
     CDS             complement(703..1038)
                     /locus_tag="ACHA6Y_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8987289.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1025..1309)
                     /locus_tag="ACHA6Y_001180"
     CDS             complement(1025..1309)
                     /locus_tag="ACHA6Y_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8987290.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1365..2888)
                     /locus_tag="ACHA6Y_001181"
     CDS             complement(1365..2888)
                     /locus_tag="ACHA6Y_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8987291.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(2881..4056)
                     /locus_tag="ACHA6Y_001182"
     CDS             complement(2881..4056)
                     /locus_tag="ACHA6Y_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8987292.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4060..4245)
                     /locus_tag="ACHA6Y_001183"
     CDS             complement(4060..4245)
                     /locus_tag="ACHA6Y_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987293.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4211..5905)
                     /locus_tag="ACHA6Y_001184"
     CDS             complement(4211..5905)
                     /locus_tag="ACHA6Y_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8987294.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(5902..6375)
                     /locus_tag="ACHA6Y_001185"
     CDS             complement(5902..6375)
                     /locus_tag="ACHA6Y_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8987295.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6444..6599)
                     /locus_tag="ACHA6Y_001186"
     CDS             complement(6444..6599)
                     /locus_tag="ACHA6Y_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM8987296.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(6733..7113)
                     /locus_tag="ACHA6Y_001187"
     CDS             complement(6733..7113)
                     /locus_tag="ACHA6Y_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8987297.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7117..7332)
                     /locus_tag="ACHA6Y_001188"
     CDS             complement(7117..7332)
                     /locus_tag="ACHA6Y_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987298.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7335..7742)
                     /locus_tag="ACHA6Y_001189"
     CDS             complement(7335..7742)
                     /locus_tag="ACHA6Y_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM8987299.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8008..9483)
                     /locus_tag="ACHA6Y_001190"
     CDS             complement(8008..9483)
                     /locus_tag="ACHA6Y_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM8987300.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(9473..10321)
                     /locus_tag="ACHA6Y_001191"
     CDS             complement(9473..10321)
                     /locus_tag="ACHA6Y_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM8987301.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10358..10720)
                     /locus_tag="ACHA6Y_001192"
     CDS             complement(10358..10720)
                     /locus_tag="ACHA6Y_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987302.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(10721..10879)
                     /locus_tag="ACHA6Y_001193"
     CDS             complement(10721..10879)
                     /locus_tag="ACHA6Y_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987303.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(11020..11644)
                     /locus_tag="ACHA6Y_001194"
                     /pseudo
     CDS             complement(11020..11644)
                     /locus_tag="ACHA6Y_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(11641..11952)
                     /locus_tag="ACHA6Y_001195"
     CDS             complement(11641..11952)
                     /locus_tag="ACHA6Y_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987304.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(11996..12289)
                     /locus_tag="ACHA6Y_001196"
     CDS             complement(11996..12289)
                     /locus_tag="ACHA6Y_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987305.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            12483..13130
                     /locus_tag="ACHA6Y_001197"
     CDS             12483..13130
                     /locus_tag="ACHA6Y_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8987306.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13191..14336
                     /locus_tag="ACHA6Y_001198"
     CDS             13191..14336
                     /locus_tag="ACHA6Y_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8987307.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(14417..14722)
                     /locus_tag="ACHA6Y_001199"
     CDS             complement(14417..14722)
                     /locus_tag="ACHA6Y_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM8987308.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(14747..15178)
                     /gene="ruvX"
                     /locus_tag="ACHA6Y_001200"
     CDS             complement(14747..15178)
                     /gene="ruvX"
                     /locus_tag="ACHA6Y_001200"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM8987309.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15175..15441)
                     /locus_tag="ACHA6Y_001201"
     CDS             complement(15175..15441)
                     /locus_tag="ACHA6Y_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM8987310.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
BASE COUNT         4437 a         3243 c         2484 g         5386 t
ORIGIN      
        1 ttccaaattt gcttccttat aatttgaaga acgaccatgt tttactctgt tatatatttt
       61 ccaccaatct ggtgtatctt caggccaccc atctccaaaa ggttttagtg aagtcaatgt
      121 taaagaattc ataaccctaa caccattatc caaacacatt aattcgctag atttaataac
      181 agctttaaaa tcagttatgc ttttattgct actgctatca aatccgcaaa gttctttgaa
      241 aataatctca cactccgaac caatcgctag tagtagtttt tgatattcaa tagagaatgt
      301 tttgaaatta ttctcatcga gcatcacaaa atctgtggta ttaataaact cattttctaa
      361 atgtagataa tacttccaat aaaaatttgt aaactctgct ttgtccatta aatcacctct
      421 tttgaattat aacaaaaaaa acacgtccac aacagacgtg ttctttgatt tctacacaat
      481 cagcttgcca aaaccatcca atcagtccta gcatgcctgt attaaaattg aacgttcatc
      541 atctatcatc tttcttagtc cagcatgttt gcaaccacac ctacaatttt cagtccaaca
      601 atagatttcg actagctaag tcttgcaaaa ttcaggcagc taacccttca tttactacct
      661 taattatact aaatacccac aacgtttaca agtgtaatca ttctaagaaa tctgtttagc
      721 tatgatcgtt gtataatctt taaacgccgt tccttcattg attgaaataa ttttataact
      781 cttgtttttc caagctactt tcatatcgtt tgttagttca taatcttgat cgtatcgaat
      841 aataaacgtc aacgttcctt caagaatggt acctacacta gcaagcacgt cttttagcat
      901 ttgtgaacgc acattagccc aacattcaaa tacagtttct tcactatcca ccatctgccc
      961 atcttcatct tttacccgtt ttgtcttttt aaacttgact ctctcagtca attcatttac
     1021 attcttaatg attggcataa taagcacctc tcatttgttg tactaatgct tgtaccgtta
     1081 ctggtatccg ttcgctgctg gcttcttgac ggttcaaata ccaatgttga gtaagtaatg
     1141 acaccgccaa atcaaattgc tgataattat ccatatcttt atccgttgcg ctactatcaa
     1201 tagcactttt tatatattcg cctgctgtat caatcaattg ttgaataaag tcatcatcaa
     1261 tagtgtgatc gatccgcata ctttttttta tgctatccaa aattaccatt ttatctctcc
     1321 taatgaaaaa aaggcagtgg gataaaccgc cgcccaatat ttttctatac ttttggtgtt
     1381 acaactggcg taaactcaat atacactgaa gcattttgat caattttctt ataatcgttg
     1441 cgaacgatca ctgcaagtcc ttgtgagtag taatcgaatt tttcccattg agtcgttact
     1501 tgatttctac gagcaacaaa gatagattgt gcaatatccc ccataatcat agggaatgtt
     1561 ccagctttag gattggcaaa caatgtatct gcaatcaata ctactggcat accgaataat
     1621 gatttaccac taggtgctgt cacatcgggt tgtaaaatgt aacgtccatc tgaatctttc
     1681 aatgtatcta ggtggtaata tccgctttgg tttagaatta ccattttatt taatgctggg
     1741 tctaatgcca cattgtatag ttgtttcaaa tcgtccaacg tagcagccgt tttcttagtg
     1801 aatgttttta ataaatccat gatatgctta ttgtcagtgt tttctaccaa tttcgctaac
     1861 tgatctttga cctcttgtac aatattcact gctgaatctt ccacaacctc atttgataag
     1921 gcaatcttac cagcacgagt ttctacttta taatcaactg aagtaaacat ttcagcgtca
     1981 atatcaccaa tttcagcaag ttcagctttt gttgctaaca ctgcttgttg gttagttgct
     2041 actggatatt taccttgtcc atttgatact gtttttactg ttgcatattg agctaagtta
     2101 taatttgaac gtttcaaatc aaagacttca ccgattactt cttcgggaac aactgccgct
     2161 gcatttacag tagtgactcc atcacgcact tcgccttgtg aacggatata ttcttcatac
     2221 ccacgcaatt ctgttttttc gttatcgatt aaagtttttt ccatgttatt actctccttt
     2281 tgtgctttag aagtggattc taagctattg tttgtttgtt ttgcttggtt gttactcata
     2341 aacgattcgt aagaacgctt atttacttgg acatttgatg aatcataagc gggaacggtc
     2401 acgacactaa tttcattaag tgctttgatt ttatctattg atcgttcaat tgtgccatct
     2461 tcttttttgt cgaatgaatc taatccaagc acgaacccaa aggacataga atcaacaacc
     2521 ccttttgaga tattctcata cacatcattt gaataggtcg tgtcggttag ctctgcttca
     2581 aaatgtaatc ctgtatcatc gacattcaat ttcaacgtac ctgctttgac actagctagt
     2641 ggctttgaat aatcgtgtcc atataataag aacacatttg ataaatccac ctcttttaat
     2701 gcttcgggtg taatcacttc tacaaagccg cctaaatctt ttgacggttg cccgaatttc
     2761 aacgcatagc ctgaaatagt tttgccgtct gttggttgtt tttcattttc tttctctact
     2821 ttgggagaat cggctgttag ctcagcttct tctgtcagcc gtttttcctt ttcttcttca
     2881 ttattcaact ggtgtaactc cttcctgttc ttttaattga tatttctcca acgtatctaa
     2941 atacgtaaag ttcaaacttg ctagtaaacg atcgccacca tccatggggg gtagccccat
     3001 tttcgaccgc ccctcattaa tcgtgagaag tgaccctttg acctgatcca atacattttt
     3061 tacttttgtt tcaggatcag tctctagtaa ctgatcggaa ttaaagcgga tattggtttc
     3121 aagtttcgta tctaattcac tcacaaacac attaaagtaa tgaatcagcg tactttggat
     3181 atactggaga ttactttgaa cggtgctaga atgttcattt tcaacgccta aacgctcaat
     3241 cggtacacca aacgccttgg caatttgttt tgtgttccaa tcattggaat tgataagctt
     3301 caaaacatcg gtgtttattt ctaatgtttt gtagtccatt gtttcatcaa gaatgatcgt
     3361 tctaagagca ttatctccac tacttgaacc gttggcttct tcaaactttt cacgaattgc
     3421 gttctttgct gatccgtcta aatctgactt atgaaccttc aaaatacctg acccactgac
     3481 accacgagta aagaaattgt gcaatgtgcg gttgccagct tgttgaattt tcagttcatc
     3541 acgtaacgca taaagtggtg gtaatcctgt taaaccatct tgggtgaaat atttgaagtg
     3601 caaaatatcg ctagacttca cacgtctttt tttatcacca atctgataag acaacgtacc
     3661 attatctaat tgagtaaccg ttacacttga gttaggtaat aagtgaattt cttctacctt
     3721 ttcaccgttg cgtttaatct ctgcaaagct attaccgttt agcaacatat tgaccgccaa
     3781 agcaaacttt aaatgccaac catccatttc tgaattaggt ttctcgttta gtaacttcac
     3841 cagttcatta tcagcttgtg gcatattgtt tttgaccaat tgaatcggac ttgaagcaat
     3901 atcactagca ataattcgaa cggctgcaaa cacatcacta tttcttaatg cacgaacgct
     3961 tgtaaagctg gtggtgtaat catccgattg gattgatacc acatgttgta ggaaaggatc
     4021 tcccgttgta ttttgtctcg ctttaaaaaa tgccatcttt taacctccct tctgttctgt
     4081 atttagcatg accgctaccc caataagcac tatgcccagc gctaacaagc catagtagac
     4141 atttgttagt aaggtgatag caaccaacac acacaccaat ccaagaagta aaagaacggt
     4201 atgaatgtaa ttagaaagaa aattcttcac ttgtataaaa ttcattgtct gctttcgctc
     4261 cttccgttgt ggtgaagtaa tccattgcaa acacataggc attgattaat gcagctattg
     4321 gatcaatttt gttactgttc tttgatttat taatctgaat gccgttgtta tccgttttta
     4381 aaatagcgtt gttcactgca tgggtgagaa tcgtattttt attgtgaata acgttgcctt
     4441 cataaacttg ttctctaaaa gttcgggtcg gaacgtttag agtaatcgtt ccttgtctta
     4501 cttctaacat tgggtagttg gctttttcag ctttactgat aagcgaatta gcgttatacg
     4561 ggtcataaca aatccccata cattccaaat cattttcttg aatcaaatct tgaataaact
     4621 gaaatacttg gtcatagtcc acgattccac tttctaattg agtaatggaa cactcaccag
     4681 ctttttctag ttctcgataa ggcaaaccgt cgcgcttttc tttgtcttga agcccatatt
     4741 tggtggctac aaagctatgt gaatcacaat aaagttcgcc gttatctagc ggtacgatcc
     4801 atgaaacgct agttaaatca tctgttttag ataaatccac tccgatataa acgggcttgc
     4861 ctgtaatatc ttgtggttct acttcggttg cctgccagtc ttcagcaatc atatagctgt
     4921 cctcactcgc ttgtctccat aaattgaagt ttttcaccaa tacagaatta aggttatttt
     4981 gtttcaaagc aaggttcaca tcatcttgaa tggttggaat cattactttt ttgatttctt
     5041 cactttcaaa aatcggattg gctttgatcc atttctcttg atcgtgaatt tcttcgggat
     5101 cgtccaattc ccatatcgct ataaagtacc tgtcggcttg ttcttcgcct tttagcacac
     5161 gatctaacaa taagtattcc tcgtacatag gaacgtttaa atcaagccca gcggtactaa
     5221 taacacacaa taagccgttc ttttgttggg tcattcctga cttaatgacg ttgtacgttt
     5281 tacgagtttt tgcttcgtgc cactcgtcaa gaattgcggt tgttggtgca aacccatcta
     5341 gcgtgctggt ttcactagcc agtgccatag cgaacgaatt agagggcaag tgctgaatct
     5401 tagagttcat aattttcagt tgtggtctta aaaacttact agacttgacc acgtttctaa
     5461 gtgaattaga cagcatatca tagcccaatt tagcttgttt caaagcgtta cttacaaaca
     5521 atacttgcct tgcttctgct ggttcttgtt ccataatcaa cgcattggca gccatgccac
     5581 ttgcgatata cgttttccca ttcttacggc tcatactggt aaacgctcga ttgaatcgcc
     5641 tgtaattccc ttctttagta cgccagccat aaagactgcc tagaatgaat ttttgaaatc
     5701 ctaacatctt gattgcttta ccgtccgtag atggtagcaa ctcaatgaat ttaatcgctt
     5761 tggtggcttg ttcttcatcg aacacataag ggaagctatc tgacttagaa cgctccaaat
     5821 cgcttatatg acgctcacac gcttggataa ttttcttggg tgcaatcagc ttacctgata
     5881 ggactttctc aatgtaatca ttcatgaatt catcaactct ttgaacgggt cttcgggttc
     5941 atcattttta accttgttca aagctagttt tgatcgtgat tctaaggtca tacctagtga
     6001 ggtggcttgt tttttcaaat cactcatagc ttgtgattga acccgaaccg ctggattggc
     6061 tttctttact ccgttttcgc atgtgataaa cgtacctgat tttcttatct cggcttcggc
     6121 tgttttcatt cgagcaaagg ctaaacaata ggctaccaat gaaccataat ctgcttcact
     6181 caatggataa tcttttttta atgttggtac gatacgattc cattcacttc tagcgctcgc
     6241 tgccatccag tcagggggcg tgatatttat taatggttgc tgcttgaaca attcttctct
     6301 agcgtccaca cggattgctt tttcttcact tgatatattg ccttttgtat cgtgtaatat
     6361 tttagggtgt cccatgttgt cctcctttca gacaaaagaa aaaggaacgt aagcaattaa
     6421 gctcacattc cttcgttttt cgatcagaat ttttctttat ctaatttatc tatataaatg
     6481 atttttccgt tctgcatttg aatggtcact tgaccaaaat gaggtaactc aatggtgcgc
     6541 tgtttccctg ttttatcagt aataacaatt gtatttttca ataaatctcc tactttcatt
     6601 atatactttt tcacttgtga ttacaagtgt aatcgttatt tgtaattacc cttatgcctt
     6661 ttcacaacgg ttatttgtac caaaatgagt aggcatcgat tgtcagtaag tttcacacta
     6721 tgccccctta actcaaaggg tactcttttc tcgtctttgt ctttcattgg ctgtcttgga
     6781 attatggtgt gcatggcata aagaacgaaa attagtttca tctaaccgtc tattccaatc
     6841 atcttttagt tcaattttgt gatctacaac atcagctttc cttatcaagc cttcttctag
     6901 gcaatcctca catactggat gatttaaccg atacaagtat gaagcctttc gccatgttct
     6961 actttgatag aattgaaaat atttaccttc gctttgcttt ctttcttcac gcttaattgc
     7021 cgtttgcttt gctttatgct tcccacaata cttctgccta taatcaatca atgttttaca
     7081 tccagcatga ttacattgtt ttttaactgc catagattac caaccaaagc gatctcgttc
     7141 gtcagaatca tcttgttccc attttgattt gtattcaata taaggcgaaa ctatcttcaa
     7201 gcacgttccg tctttaaaat taactttagt gattttatct tcctcaaaac taataaagtc
     7261 tacatcttct tgagtgtttg aatactcaat gtgtttctca ccatcgaagt atctaatact
     7321 ttccatatgc ataattattc tacctccaat aaattagtta tcaattcatc atctgttagt
     7381 agcaaagcca agaacttatc atggatgttt aaaaacaaat tatcctcatt cgatgtaagc
     7441 tctcgttgtc tcgctcgatc atataggtta agcagacaat taaattcatc cattagctca
     7501 cctgtatcat catagttgcc actatctacc aatgatccca ctactagcga ttgacaccac
     7561 gctactgact cctgcagcca atcaggttct tcttccatta tctttaatac ttctggatca
     7621 aatgtggaat aatcttctgt taccaatccc acactaaatg ccagcgtgtt cattagaaac
     7681 cgatccattt catctgtata ttgaaagttc ggtgctttgt ttgttgaaat aatattgagc
     7741 atttactttc ctccttgatt cgtaacacgt ggtaacacct acgtaacagg tacccgttac
     7801 cactcaatcc caacggggac aagggttcta gcgttttggt aacaggtaac gggtaaaatc
     7861 cagtaacatt attccccagc atattagcta actatttcaa aagttttaag gaaataaggt
     7921 gttaccccgt taccaaattg ctgtatccct tggggcagta agggtaaact agtaacgggc
     7981 taggtgttac tgacccgtta ctacccgtta ctttttgtac tttccagctc gatttttatt
     8041 ttcataggca tttgacttat tgtaaaatcc tcgtcgttta tttctttttg gcagttgctt
     8101 ccgttcccaa ccaggaatac tgctaataat caaggcaatc tttttagttt ctgcatctga
     8161 tcgaccattc aataaatcct ttgcttgttt atcaaaaaca gcttccaaaa tatcagcggt
     8221 gagtacgcta tcaactaaat aaaactcttg ttgcatagta gctccattat agaaatgctg
     8281 taaatttttc tcattacatt tactgtcagg atagttctga taataagaac gtctcgccca
     8341 cgctggagct tcttcccatt tatacgggat ttccatttcc aaatatttag tgattgcttc
     8401 tttaattgga ttctccatct tggcatcctc ttgatagtct tttgcaattt ctaagacttc
     8461 tttatcttca tcgggactaa aatagattcg ttgtccttta tcaaaaagca cctttgcttc
     8521 agctaacact tgaagaaaat attcatcctt tgttttaaac ggattcatct ttggcttatt
     8581 cttacaaggc aaaggataaa aacggcggtt tcctgtatca tctttcaaat actgcccatc
     8641 attcgacgtt ccaataaata cacattgcct tgcccaagct atgacattcc gaccataagg
     8701 aggtctaatt ttatcgatct tggcactgat gaagtttttt atctgttcaa ttttagtttt
     8761 gttcattgaa gataattctc caagttcaat aattacattg ccttgaagct gcatataatc
     8821 atctttcttt tcacctagcg aatctaatga atcggtgaaa aaatcatccg tgtaaagtaa
     8881 tgctggaacg gtactcttac caatcccttg cggtccatcc aaaactggaa cgatctcaaa
     8941 ctttattcct ggtttataga tacgtgcgac tgatccgact atccaacgtt ttgttacttc
     9001 tcgtgtgtaa aggttgtcgt ctacccctaa aaggtcaata aacagtgttt ccagtctagg
     9061 ctgttgatcc catttaactt tttcgatacg ttcttttact gggttgaaaa cattctttct
     9121 agccacattt actactgctt tgtgtatttt gttgtcatca aaaactacat tgtagttttc
     9181 ttcaatatat gccgttaagg catcctcaaa actgtcttcc cattctccag cggtacgatt
     9241 gatccaagga gaatctttta ataggtgaat gtatccgcta aattcgttat aagcaattgt
     9301 tccagctaaa ttgctatcat tggttaaaat caattctgca ttcacaacac tgttactttt
     9361 aatctgatcg tctttattgc atcttaatcg tgacatccag gttttcaatt caatgatatt
     9421 gtcttcacgc tcgctggctt cgggaaagtc taaaatacta tctaatttat gcttatgagc
     9481 catgagcttt ccccctcaaa tcttttttaa tcatcgatcg aataatttga ttcacttctt
     9541 tatcttttag cggtggttca ttgtacgcat ttgctgtata aatcaattct gctaaagttt
     9601 gattatctac accgtgccat agtaaccaac cgcataactt agttaaatga tcgtttctac
     9661 taccttcgcc agcgccttga acaatcatat ctaatttttt tgctgtccct gtttttgcta
     9721 cgggtcggta ttgagtcttt atttcttgcg gtttttttag cagtaggttc actagccaat
     9781 cgggagctgg tgcaatatta tttagagata cgttttcaat aggttcatat gctctcccaa
     9841 cacttcttga aataatcata gttggcgcta ttggaactcc tttagtgata atatcaatcc
     9901 cgcttaaact tttaccttta tcatcacgcc cgaagatatc agcaaatgca cctgtttctt
     9961 gttggataat tacaccatca ggaattttaa agaagaaatg gattccttta cctggtgtga
    10021 tttccgtata agtagaagga aatggatcat acattgaagc aagtttttta taattctcaa
    10081 cgccgtttcg tgtttcagaa tgttgatcta ggtcaactac caccatttga tgatcttcaa
    10141 gcattaaccc aatatttcta gttgcatctt ccgtccacca ttcattaatt tgattagaat
    10201 cgttagttgc atcattccaa ccactagagc cttttattgg aattttagtt ccaggagata
    10261 agggataaat tgaccatccc gacttttgta attccgttgc tgcttgataa ttatttgcca
    10321 ataaacctct cctccttttt caaaatactg gtataaatca ttcagacacc tccatgagct
    10381 tcaactgctc aagttcgtat ttcaacccat cccgaacaaa ttcagcacct tttaattgat
    10441 tctgatactt tacccatgta ttaaatgttt tcacccttgc ttcttgcata tcagtaatgt
    10501 ttaaccatgc ggttaaaatc tgatttttta gctcgttgat ttctcgacaa attattttgt
    10561 gttctgcatg ttttttgagt acatacaaaa ctgttagttt ttcattgaat gaatttccaa
    10621 agtaggtttt cccgatatta ttttctagtt cagcgatccc ttgattaatt tcctctaatt
    10681 caaggctatc gagttggttt aatagttttg ttttgtacat ctacttatgc cctccagttc
    10741 ttaattgata gtcgtactcg tcataagcag tgaagagata taacgctaat cctgcaaaga
    10801 tagctatttt tgcaattaat ggtaataagc cgaaaattcc gccgattaaa atgcttccta
    10861 taattaagtt tcgtttcata atggttccct ccgatttttt aatatggtat aatcggaaca
    10921 aagaatatcg ggttttatcc caattaagtt gcctttatcc tgtccgccaa gactgttatg
    10981 gataaggctt tttctttgtt ctcgatcatg ttaatgctcc taagttgtct gcaattcagc
    11041 agaacgatta taaattttct cactcactga aaactgcaac ccatattttt ctttaacagt
    11101 cattaattct actgtctcat ctaaaatagg ttcacgatca accaacattt caggagtcat
    11161 atctttcttt tttaccattt tggaatgacc ataaatagtt gatacagctt tattggctat
    11221 cacattcgct ttaatgtagt ctactggttt tggttcagta atagcgtggc ttaatttagt
    11281 cattgcttct ttttggtgtt ctttgtctag catgcggaat acttgaaagc cttcaagtcc
    11341 tgtggactgg cgtagttctt tgatgatgtc gaaaatccag tcttgaaatg cctcggcttc
    11401 ttttttacgg cttttgaatg ccatgcggta aatatttttt ttcgttgatg atgtttacat
    11461 cttgtgttct gcccaatgaa tcgatgacct tactagtagt aaccccatct ttaggcaaag
    11521 agtgaatggc tctagtaacc tgtttaagac ctaacgcttc cgcaacatct ttcgccactg
    11581 cccaccattc atcattaata tcgacaaatc ttataatgtg tccgttccaa atttcagttt
    11641 tcattcaacg tcctccagtt caagccctag caagctcgct aacaactcaa cttgctgata
    11701 caaaatttct tgatactctt caatagaaag tcgttcttta aactctgctt gtactaaatc
    11761 gttcaagcca cgcaatactc cgcttaccgt tggttcttgt ggtagcaatt tttgatagtc
    11821 catttgttcg tacaaacatt caaacgtttc ttctagcgct ttgccttcca agtttgctaa
    11881 ttctgccgct gtgtcttctg taagaattgt tgataatact ttttcgattg ctttattttg
    11941 tgtcgattcc attattattt cctcctatta atccctttgc ccccacggct tgcgtttatt
    12001 tttggtattc ttccataaag cgatcacatt catcttgtga aattcttacg actccatcaa
    12061 ctataatcaa ctttaaaccg ttaggaacat atttctgcat catagtattg tatgaaacgt
    12121 tcatatactt agcagcttgt gccttgttca tatattttgg aaactggtac tttttttcta
    12181 ataatgaaag taacgttcct tgagtttgct ctacttgttg aacaactcga tcgtcaataa
    12241 ttttaaaagc tggatcatca agcgtgagtt tgtcaatttt taattccata tttatccccc
    12301 ctcgttagca tacatttcta tacaaagatt ttaaaaaaat gcaagaggct cttgaataca
    12361 tttctatacc tcttataaac tcaatatacc atcatacctt ttcatttgca agaattttaa
    12421 catacttttt tgaactttta tgtttcacac atatttaaat cgtgatatag tagagaaaat
    12481 atatgaatgg tggtgaaaat gtggctagaa aaaaataccc cgaagcagtg gagacaggac
    12541 aaagaattag actggctaga attgaaaagg gactctcaat ggaacaactt ggaggtaaat
    12601 tatccccacc ggccagtaaa ggtgccgtta gtaattggga aaatggctat aatcttccca
    12661 ataacaccag gcttaaacaa ctttcaaagg tattagatgt atccactacc tatctattaa
    12721 acggatctta tacattaaga gatatgcata tgatgcccga aattaagcga aatgtgcttg
    12781 aaaagcaatt gaaaaaaact attgaaaaaa ataactctat aattgattct aatctttcta
    12841 atgctatcaa taacatcgaa gaagataacc tgactgtctc acagaagatt tatttaacaa
    12901 acgcaataaa atatcttaaa acaggatctc gaattgaatt tcctgacggg agtaccccaa
    12961 ttgcttatct cgcagctctt ctaaatacgc ttttacgata tgactcatta attaatgatt
    13021 cagaaaaaaa tacagatgag aaaaaagagt atcaagaatt ttatgaaaat gatcttctaa
    13081 aagagttcac agctcttgtt actgagctaa ataaacacta ttcaaaataa taataaactg
    13141 cctcctccaa ccgcccccac ggcttgctac actatggagg gaaaactatt atggcaacat
    13201 tcaagcaata tgaaactaaa aaaggtaaat tttggctcta tgaagcctat cttggaatga
    13261 acaaaatgac tggcaaacct gataaggttc gtagacgagg ttacaaaaca aaaaaagaag
    13321 cccagcttgc cttgtcacgc ttgcaagtgg actacgataa aaacggtatt aaaaaagcga
    13381 acaacgaaac gtttaaagaa gtttatgact tgtggctaga aacatataaa accactgtcc
    13441 gagaagccac cttcatgaaa actgaaatca agtttagaaa gtggattctt ccaaaatatg
    13501 gaaatcttcg tgtgaatgaa gtcacggtaa aaaaagccca acaaattgta aatagctggg
    13561 ctgaaactac tgaccaatac aaggtacttc attcaacttc taagcgtatt tttagctacg
    13621 ctatcaatct tgggattatt gagactaatc cactagacaa cgtattaatg cctaaaagaa
    13681 ccaccactaa gcataaagaa aaggtcaaag tttacagtaa agaacaactg atcaccttgt
    13741 ttgattacct aaacagcaaa cctgaaactt atcgaaacga ctttgataaa acgttactac
    13801 gctttctctt ttatagcggg gttaggatca gtgaagccct tgcattgaat tggtcagaca
    13861 ttgattttca agaaaatacg gtcaccatca ataaaacact aagccaatcc aaaaatggct
    13921 ataaaatcag tgatcctaaa actgattgta gtgctggggt tcttccacta gatgaaagaa
    13981 caatttctat attaaaaaaa tggcagatta atcagcggaa atatatgctg acacttggtg
    14041 taactgatcc gactatgatt ttttgtggta tctataaaga actaatcaca caccacgcta
    14101 tttatgcaag attgattact atttgtgaaa aagctatggt tccatttctc ggtgtgcatg
    14161 tagcaagaca cactcatgct tcaatgctct tggacgctgg agctactatg gtagaagttc
    14221 aaactagatt acggcataca aaaattagtc agacgatcga ttgttacggt cacttggcaa
    14281 aagaaacgaa agaaaagact gttgaaaaat tagtacaaca cttaaatatt gactagatgg
    14341 catacaatat ggcatacagt aaaaaataaa aaaaggagaa accctatcat atcaaggttt
    14401 ctcctagctt tatagtctat tcttcttctt ccgccatgaa tgtattgaat acttcttcaa
    14461 tcatgtccca ttctgcgtct gtttcgattt gttctaactc gccttctgtt ccatcttcgt
    14521 tttcgatgta tgcataagct tgtaattcta catcttcatc ttctgaaaca ccagcaggat
    14581 ataaaagaac atagttacgt ccaaattctt cttgtccgtc aactgttagt aggatttcat
    14641 ataatgtttc atttccttgg tcatccacca atgtgatgtg ttcgtgtcct tcatggtcgt
    14701 gggaatggtt atgttctgtc ataagattac ctcttttctt ttattattaa aataattgct
    14761 tacctgagcc gtctaaataa ttttgtaaga tcatcacagc tgctaattta tcgatgactt
    14821 tttttcgttt ggctcttgaa gtatttgcct gctctactag catacgttct gcctgaacag
    14881 tggttaatcg ttcatcttga taatcaactg gcaaggagaa tagttctttg atcttttctc
    14941 cgtatgacat ggaagcttct gcccttggtc cgatggaatt gttcatattt ttaggtaatc
    15001 caactacaaa ccggtctact tcatattgtg ctactaattc cttcaaccga tcaaagccaa
    15061 actcttcttc tgcttcattg atccgaatga tctctactcc ttgagctgtc cagccaaagg
    15121 ggtcactgat agcaacacct actgtcttag aaccgacatc caagcccatt actctcataa
    15181 attcactcca tgatttgaga ggtagtcctt gacaattact tccatgatct catcgcgttc
    15241 gtgacggcga atcaaatttc gagcatcacg ataacgagga atgtaggacg ggtctccaga
    15301 aagcaagtag ccgacaatct gattgattgg attgtagcct ttttcttcca aagcccgata
    15361 aacaatcgct aatgtttcgc tcacttcttt tttgcgacta tcgtcaaaac caaaacgtac
    15421 ggtttcatct gtaaaaccca tgattctaca cctctttccc gcaaggtaat tccagcttac
    15481 cttctacaac cttattttac tcgaagaaag ggaaaactac aaacaaatat tccaaacttc
    15541 cgaataaatt
//
LOCUS       DAYYHX010000051        15437 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000051 DAYYHX010000000
VERSION     DAYYHX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.051"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(10..1374)
                     /locus_tag="ACHA6Y_001202"
     CDS             complement(10..1374)
                     /locus_tag="ACHA6Y_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8987311.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1371..2930)
                     /locus_tag="ACHA6Y_001203"
     CDS             complement(1371..2930)
                     /locus_tag="ACHA6Y_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8987312.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(2986..5466)
                     /locus_tag="ACHA6Y_001204"
     CDS             complement(2986..5466)
                     /locus_tag="ACHA6Y_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987313.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            complement(5450..5785)
                     /locus_tag="ACHA6Y_001205"
     CDS             complement(5450..5785)
                     /locus_tag="ACHA6Y_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987314.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5799..6833)
                     /locus_tag="ACHA6Y_001206"
     CDS             complement(5799..6833)
                     /locus_tag="ACHA6Y_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8987315.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6923..7603)
                     /locus_tag="ACHA6Y_001207"
     CDS             complement(6923..7603)
                     /locus_tag="ACHA6Y_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8987316.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7887..8990)
                     /locus_tag="ACHA6Y_001208"
     CDS             complement(7887..8990)
                     /locus_tag="ACHA6Y_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM8987317.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(9212..9682)
                     /locus_tag="ACHA6Y_001209"
     CDS             complement(9212..9682)
                     /locus_tag="ACHA6Y_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM8987318.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            10015..11655
                     /locus_tag="ACHA6Y_001210"
     CDS             10015..11655
                     /locus_tag="ACHA6Y_001210"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM8987319.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            11809..13131
                     /locus_tag="ACHA6Y_001211"
     CDS             11809..13131
                     /locus_tag="ACHA6Y_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM8987320.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(13193..13624)
                     /locus_tag="ACHA6Y_001212"
     CDS             complement(13193..13624)
                     /locus_tag="ACHA6Y_001212"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8987321.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(13688..14524)
                     /locus_tag="ACHA6Y_001213"
     CDS             complement(13688..14524)
                     /locus_tag="ACHA6Y_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM8987322.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         4513 a         3115 c         2795 g         5014 t
ORIGIN      
        1 tgatatttct cacagtcttt tatctactct attgctattt gctctcttag ctagtacttt
       61 aaaagctaac tcattgaggt agtgaaaacg agaggtgtat tcagacagaa atccaaaatc
      121 ctcaactgct agctgtggaa tcgtagtttt gtgtttcacg atatccgcaa gcaggttcaa
      181 atctgataat aagaattctt ggcttatcag ctgtccgcca attatttcta atgtatccgg
      241 atggtagatc agctttgcat ttactgtttt gttttcttcg tcgtattgtg gaaatagatc
      301 aaattctcct gaacacgaac cggtattttg ttccaagaat ggcgcttcat cttctgtcag
      361 ccctacagtt cctaaaaagt agtctataat gactgctgct gttatctttt gtctgaggtc
      421 gtaagcggtt ggctgaccac tcaaagttag cgctactgct cttgccatat gtacagctcg
      481 agtaactaaa ggtaaaaaga gatgccgttt atttttgtca aaactgactg ggacgatatc
      541 tccaatcgca tagatgtccg gatcacttgt ctgcagatat tcattgacta tcacagtctc
      601 atcctcatac aaagccactt tttcttttaa caatgtcgta ttaggacgag tttgggcaga
      661 aaaaatgacc atatctgcct ttattgtttg agacagaagt tgtacggata ctactttctc
      721 ttctgatttt ttccaatcga tcaagaattc atttggataa aaatttattt gcttagtttg
      781 cagctgattt tgaaaactgt tgatcatatc cttatcgaaa tatcttgata aaacattcgg
      841 catataatca actagatgaa taatgtatcc tttatttctc aaagcatctg ctaattccaa
      901 tccaatgtaa ccagcaccga agatcactac ttcttttgct tcttttaaag cggcaagtac
      961 ttgcttagat tgcactagat ttttataggt gtagacattt tcagcttcaa tttgtttata
     1021 caaggtgaca ggagaggaac ctgtcgctac gattaattta tcataggtta tggtctcgtt
     1081 ttttccttct ttggatgcga ggatgagctg atgtttgtct ggatcaagtt cagttactct
     1141 ccattcggtg atcagttttg ccccactttc ttgaagagtc cgcatgttat gggcaacttc
     1201 agctaaacta cgtatctttc cttttaaata aaggttgatt ccatttgaaa catagcctag
     1261 tccatcttta tgatcttcat cgatcagtaa aacttctgct ttcggttgaa gttttcttag
     1321 atttagggca gctgtgatgc ctgcatggga agcaccaatg atcacgattt tcatgtttta
     1381 ttcatcctta aattcaattt ttctaaataa cattctttta aaaattggtt caaatagggg
     1441 aaatcataat tatattcgtt gctagtgaat acaaaaactt tagcggactt ctgtgacggg
     1501 tactctttca attggctagt caaaatgata tcatactgct tttctttctc gtattcgact
     1561 aaatcatatt tgatcgtatt cagcaattgt tcctggatga tttctttcac gatatccgcc
     1621 attagccttt cataaggaag atcataagca atttgcagac gtaattctaa ctgcttgatc
     1681 agcgtccgta aaatagatgc atagcgagca aataattcaa agcgggcatt ttcctcgtct
     1741 acttcagtaa aatcaacaat agcaaatgtt tgtctgacca atctttcaca taaaaccagg
     1801 aattgtttat cttctacttc agttaaccgc tgttttaatc cttcttggcc aaaaatattc
     1861 atccatcctc taaaataggc aaaatgagcg tgattttgca tcactgtcaa cgcaatcttt
     1921 tgcgaaatct cttctggaaa aacataaaaa gagaaatatt cttctatcga ttgtttaaac
     1981 gttcgattca attgagctac tacgtcgtct ttgtattttt cgcttatcaa aaactcctgg
     2041 acatgcgagt tatgtgtatc taatgtaaag ttactgatca tgaaaagata aaaaaccaac
     2101 gtttcatttt gattccaagg aaaggcatat tgaaagaagt aacgggtcaa aaatgtatgg
     2161 aggtccaata aaaattgtgt cgtttgaaac aatccaacag gaagattgct ataatctggt
     2221 ttggattttt tcgtacagcg cattcgcttc ttcataatgg aaagccatat ttcaaatttc
     2281 aatcgtcctt cttctgtaat agcaaaacta aactcttttt gcagcagatc taaaaaagtg
     2341 tttcctgata aatcactgta ttcttttttg ttttcaagat agggacgtcc aaaccaaaat
     2401 aattggaaat aaaaataacg aatttgactt tctggaccgg tcagccgccc gctttttatt
     2461 ttcagatgaa actcatttaa agaatcattt aattccttca ctctgcgaga caaagtagcc
     2521 gaactgatat taagttccat gacaagtttc tgccgatcaa atgagccatt ttccaacaga
     2581 taaagaagta tctgatattt tgtagaatag cgcaagtaac aatgataata aacatcgact
     2641 gctgctatat ttccatgtct gattaataaa atattccctt cttggataac aatttccaat
     2701 tgtttgatat tttccaagtt agcttgtaaa taaaacagat cttctttcaa ggtagcccga
     2761 tcaactgata agcgggtaag tacgttatta acggaaacct ctcctttttc ctctaataaa
     2821 agttggaaaa gtttgatttg ccgttgttca tttttttcaa gcaaactgaa aagttccaag
     2881 tccactcctt caagcatttc tgattttaac agcagaagtt tccttctcat ccttgaaaaa
     2941 gaggatctgt gacagttatc acagatccat acaaattcta atcacttagc tggttggccc
     3001 agctgtcagt ttccatgtga ccatgccttg atacgttgcg ccaggttgag cattcgtact
     3061 gctatttacc tctaagagta aaccatcggt atcgggaaag tcaacgagaa cctctccttt
     3121 agctgttcca tcactctcat aaatcaacgt gttttgacct tgctgaatca attgctttga
     3181 tccagaatga ttgaagtata attttgttcc gcttaattct tgaccttgat cattcttaaa
     3241 taaagaagtc aatgtacctg tcagttgcca aggagacgtg cttggaagcc gagtatcccg
     3301 cacatcaaaa gaaaaatcgc cttttgattc ataataactt gtcttcaacg gtttggggag
     3361 atcgttacca aatgtaaaat catctggtac attaactaat gttaattctt gtttggggac
     3421 aagaattgtt ttagcagatg tttggacaac tgggttagga agcttagcac catctgctaa
     3481 tgtaatattg ctggaaagta cagctgcctt tgtcagtatg ctgacggatt gtaaagaagt
     3541 atctacagta tttaattgat agttgtaagt aagatgaatg gtatctccta ttttgctcaa
     3601 tagatctgct gggacagaaa ctccttgttg aatgccggta tctgcgatag ttgtaccatt
     3661 taattttaag gaaccattga tataagtagt attttcagga agtgagatcg tgattttttt
     3721 gtcggttatc gcatccccag attttgttaa cgtgaatgtc acattttctt gaatcgtatc
     3781 tcctgataga gcagacaggg gatctcctgt ctcaggttgt ctgctaaggt tttttagctg
     3841 actatctgca ctcacagatg gaaaattagc cactgttatt tgaggaagag tggtgattcc
     3901 agcttgttca gactgccatt tgtacgcaaa agacggatac atgttagtaa gtacatcgtc
     3961 caccaagcga taatcaaaat aatattgccc tgaagccaat gcaggtagtg tagaagtagc
     4021 tgtatttaca cccccgttat ttgattggat cgtttggaaa agttgatctg tccatcctgt
     4081 cgctaatttt tgggttcctt tcgaataaag cctgtaatgg atcgaaccct tttttagttc
     4141 agttgctgac caattggccg tgacttggta cgatgcagaa gaaaggagct ttccccactg
     4201 actatcggct gttaagtttg ttggtaaaac cgttccgtct agcttgtact gctccaatac
     4261 ctttaaagca acaggagaaa gcaatccgcc ttgcgcatct ttcccactgt acacgatatt
     4321 ggctgtctga ttcaatccac ttgtgcttgc tggagtatga gtcagtattg ctccttgtgt
     4381 aacgtttttt cctgtaaaat agttgactac gcgatcaaag cgactaaatg gcaactgatc
     4441 aaaacgtcct tcagtcagcc ccatcgtcaa tgtccgacta ttggtagagc ctggaaggtt
     4501 ctggttattt ccatccacgt attctgtggt atcagcaaat gtattatatt ttcctgttga
     4561 atcacttggt gagatgtaac tattccacgt atttatctct gcacttgcag aaaatgcata
     4621 tgctcctgga ttcaagtttc cgccagttaa tttaggataa aatctacctg cagctggagc
     4681 taaaatcgaa ttgaacatac gatcttggat aatattattc gaatcataaa aatcaacaga
     4741 aaaaacggcc tttttagaca tagaaggttg taatttaccc ttggtcaaaa ttttgtttgg
     4801 actcgtatca tatttatctc cgttttctgt catcttcgcg acattagctg taacatcaaa
     4861 ttgaagcggg atatcgtcca cgttgaattc gcctcgcgta ataatagcaa taatatctgt
     4921 caatagagta tttgtatccc acgaccccat cccaaaacgc aaggtatggc ttcctgctga
     4981 taataaactt ccgctagtaa ccttgatggg ggcaccggca atatacaagt aggcgttgtt
     5041 ccagtcaatt gcttttaata catcactgct tgatacatgt tccggcaaat tgatgatcgc
     5101 cacaaatgca aatccttgtg cagtgatatt caccaaatca agtacatttg ttagtttcac
     5161 atggctttcc caatgaaaac ggaccgtgcc acctttccaa ggatcatttg aaccatcgtt
     5221 caaataagta gcagtcaatt taggcaattg gttagcccca gttagttgga caagcttccc
     5281 gccattgact gcactacgtc caccatttgg aggtacgata ttttcatacg tgtatggatc
     5341 ataagaaatg gcaggaggcg ttgctccaac agcttctgcc gaaggtgaaa agataaatat
     5401 acttaaaaca aaaagcgaag taaaaaagaa ggagcgaaaa aatgaaaatt tattttgtag
     5461 caacatgttc atcctccttt tcctttcttc tccataacaa caaccacgca agaagccata
     5521 attctatacc aatgatcaaa taaagaaaac ggatcttttg ttcattcgtc tccgggaata
     5581 cgccatgttg attagattct ttataaatcg gtagctgtgt ctgctgtttt gatgcgccat
     5641 tgactgaacc acttggatgc ggaattggac ttagatcata aaatgtaatc gtacctgaac
     5701 ttttggtatc tgcttggact ggctcttgcc agctgaagaa cagcaaactt gcaataatcc
     5761 ctcccaataa gatcgatgtt ttcaacgaaa tcacctcatc atgttgtttc tccttttttc
     5821 tttttcttgt ggacccacaa aaacagcaaa atcaaccata acactagaac tgcagcaacc
     5881 aatcctgcat aaagccatgg gaaagattgt tcttgtactg caaaaacgga attttcattt
     5941 actttttgtg ctttttcttt tttgatcgta aagttttgtg taaatcgcca ttcattactg
     6001 tcgcctgatt ttgctatgat ttctgcgcgg taatcgccgg cttttgcttt atcaccagct
     6061 aaatcaagat tcaagtgaaa attcgaattt ggtgcaacta aaaatgcatt agacgtattt
     6121 tcaatataaa gctgattttc ataaaagatc ttgctcgtaa atatcaaatt gctgataatc
     6181 ctaggctgag gattttggaa acgaagctga acagttggct ttgcgttgac ctcgtccaaa
     6241 tctcctgata aaagtttcag atccggttct atcgtctctt gactttcttg taaaacgact
     6301 gcgagattat aagcaatacg atttttgatt tgtgctcctt ctgtagcatt ttctgtttca
     6361 gcttcttctt taggtgaaac atgaataccc gctaatacac gtcccttaaa gctttttgct
     6421 gggattttca cttctatagg tacagttgtt tctgcatgtg ctgatacatt gatttccttt
     6481 ttcgcaatga gcaaaacgtc acggacatca aacggcaatg tctcatctaa cgttgggttt
     6541 ttttgactat aatcgatcgt tccaccatca ctcgttaccg ccggattgac agagacctca
     6601 aatgttcgtt cttcatctga agaattgctg ataattactt ctagtgtctg attttgttcc
     6661 ggtgccacta ataaatcaaa ataaccgatt gctttatcta tttggttctc tggaaatacc
     6721 ggtttgattc caaagtctcc tgctgaatcc tttgcttggc tgattgacgg aaaaatgaaa
     6781 aatagactta aaatagaaaa aaacgaaaca aacagtcgtt tactttgatt cattgtccac
     6841 cacatccttt caacaaaaac tgcggtaaaa aatccttcac ctatagtatt ttcaccgcag
     6901 tttgttgaat ttatttcata ctctatgcta ctgggccagc cacgaggttc caagtcaaat
     6961 cagccgtgaa tgttccagct gctgctttcg gcactttcag tgtgatattt gctggatcat
     7021 aaacagaaag ccatgttccc agaccttgat cttttgctgc tgtaaatatt ggtgtcgctg
     7081 cacttgcttt actcaaccca gttacagttt gactagttgg tccagtggat tcgttatctg
     7141 acgtactttt aacagtggat gttccaaaat ctaattcagc gccttgcaaa acttgtgaac
     7201 cgtttacaaa atctgaaacc gaaactgtca cgttccaccc ttgtgcttgg ccatctgcac
     7261 ctacacctcg acgatctgat acttggagat aaggtgtatc gttttttgaa gcgtctactt
     7321 gatacgtttt tgttccgctt tcaatctcgt gtgtaccaaa tggcaattcg ggaactacat
     7381 ccagtgttaa aggacctgtc tggccagttg ctggattttc cggatctgct ggctctactg
     7441 gttttgaagg atcagtaggg ttaagaggct ccacggtatt tgttggttca gtaaaggtaa
     7501 tatctccttt tgaagtcgct tgaccctcag caaaagctgg tgttgccaag ccgcctagaa
     7561 gaagtgttga aagtaataat gtcccaatca tgttcttttt cataataaat cactcctttt
     7621 tcttagtgac ctaagtatag tagagacagt atgcaaaaaa tcctcatgaa ttcatccgct
     7681 tacttgaaat tatgaccctt tttgtataaa agagaaaaaa acaataaaat cgtttacatg
     7741 aatattcttc cttttttatt aagtatgaaa aaatatttca attttatttt ttcgtttcaa
     7801 attattctat tagtgacatc cttgaactat aaaaatagga gtacggcatc atctgccgca
     7861 ctcctatttt tttcgtcatt tgtttcttaa ataaaacgat tctcacgcaa ttttattttt
     7921 ttgatatctt ctactgtttg tgttccagct aattgcatga ccatttccaa ttctgtttta
     7981 aagaaatcaa acacttgatg gactcctgta cttcctccta aagacaatcc ataaataacg
     8041 ggtctgccaa tagcaacaag atctgcacct gatgcgattg ctttgaaaac atgctgtccg
     8101 cgtcgtacac cgctgtcaaa cacgattggt acacgtttat ctactgcttc tgccacatat
     8161 tgcaacgaat caaacgctgc tggaccacca tctagttgac ggccaccatg attagatacc
     8221 cagattccgc cagcaccaga ttctaatgaa cgatagacat cttcttctga ctggacacct
     8281 ttcacataga ctggcaaatc agaatgagca gcaataaatt ctacatcttt tggactaagc
     8341 ttctgtttgg aagatttgta cacagcatcc atcgtttggc ctactcctga ttgataagcc
     8401 tgaacgatcg gcattggcag tgggaaggta aacccatttc tgcggtctgt ttcacggttt
     8461 ccgccaactg ttgcatccgc tgtcaatacg attgcttttg ctccattacg cttggccact
     8521 tcaagaatat ccaaattgat cccatcatct ttgctcatat aaaattgaaa ccattgcggt
     8581 gctttctccc cgcctgcttc tcttatctct tctaatgtac aagaagcgta agaacttgct
     8641 gtatagatcg tcccaaaatc tgctacacct tttgcagaag ctttctccgc cttcacatga
     8701 gctaagccat gagcagcaac tggtgccatg atgattggtg cagtcagcgt ttctttgtca
     8761 aaatgtgtag ttgtatcggg taattctaca tcttttaata catgaggaat aatcagctga
     8821 tgattgaatg cccgttcatt ttcttgataa gtaaataagt cgccagcacc actgctgatg
     8881 taaccataac caccagctgg aatcacttgt gaagcggcta tttctaaatc ttccatattg
     8941 ataaaatcca ctattccttc atttgtacct gcttgatacg ttttttccat ttcaatgcct
     9001 tccctctata aaaaattgaa tgaataatat gatagcgttt acacacttta tattactctt
     9061 ccgtattgat acaggcaaat aatctgttta catttcagtc attccagttg ttactactct
     9121 gaaagaaatg tttagaacac aaatagataa aaaacgatgg gactttgaca aaagtcgtgt
     9181 caaagtccct tttacgaaat actgtggatg cctattgaaa taagtgcggg ttgtttcttt
     9241 gtatttcaat cgtttcttgc cattcttcgt ttaattttgg attaccgttt tcttccttga
     9301 acgcataagg tcccaatcca aaaagctcag ttagtatttg attcacgatt ccatatagct
     9361 cggtgtgatc ggacacatga ttattgaaga tcatctcatt atcaccacgt ttgaatctca
     9421 cattcgggct ctcatgatga acctctatgc agttttcccc tttttttaac tgacggattt
     9481 ctgaaaacat ctcataaata tcttctccaa taaaaccttc agcaagcgcc agactgtcca
     9541 caagaccgcc tactgtcaac tcagagatta tcgctttgtt ggcaccaagc tttaatgtat
     9601 agcttgttgc ttctactacg ccgcatgcat tgcaaccacc ttctgatagt tcgctttttc
     9661 tgatcaattg aacttctttc attttctgtt cctcctctta gttttctact tatgtatttt
     9721 ttatcacctg acgtaagtgt attttatcgt cacgatcacg tgatttattc ttcagagatt
     9781 ctatttatct atctaacata tcctttattc ttctactttt ctttctttag catataagag
     9841 atacgcatag taatcaagca aggattgttg atataaaaag aacaaatacc gctattttga
     9901 tttcttttga ctctattact catataaata aaatttatta tacctgctct ttttgactat
     9961 atcctcatct gactcctata cttcgtttgt attaaaactt aggaggaatc attcatgctt
    10021 acagggctaa acttattaaa taatcctttt ctaaataaag gaacagcttt tacaaaagaa
    10081 gaacgggcaa aatacggaat cactggtatg cttccaagta ctgtccaaac attggaacaa
    10141 caatctgtcc aagcttacgg tcaatactta agcaaacaga ccgatttgga aaaacgtatc
    10201 ttcttaatga atctattcaa tacgaaccgg acattgtttt ataaattgat gggcgaacat
    10261 ttagttgaat tcatgcctgt cgtttatgat cctgtcgttg cggattcaat cgaacaatac
    10321 aacgaaattt tcttgaaacc acaagatgct gcatttcttt caatcgacga accagaaaat
    10381 atcaaagcaa gtttgaaaaa cgcagcagat ggacgcgaca ttcgtttgat cgttgtgaca
    10441 gatgcagaag gaatcttagg tatgggtgac tggggtgtga acggtgttga catcgctatc
    10501 ggtaaattga tggtctacac tgctgcggct ggtatcaatc ctgcacaagt cttgcctgta
    10561 tcaatcgacg ctggtactaa taacgaagaa ttattgaata atccattata cttagggaat
    10621 cgtcatgctc gtgtagaagg cgaaacttac tacgaattca ttgatcaatt cgttcaatca
    10681 gctactgaat tattcccaga attgttatta cattgggaag attttggacg aggaaatgct
    10741 gcaacgatct tagaaaaata cgaagataaa atcactactt tcaatgatga tatccaaggc
    10801 acaggaatcg ttgtactagc tggtgtatta ggtggattga atatatcagg tgagcaattg
    10861 aaagatcaaa cgatcttgac atttggtgct ggtactgctg gtgtaggtat cgcaaacatc
    10921 cttttagatg aaatgatccg tcaaggtgta cctgaagaag aagcacgcca acatttctat
    10981 caagtagata aacagggttt attgtttgaa gatacaaaag gattgactcc tggtcaaatc
    11041 ccattcgctc gtaaacgctc agaattcact aatgctgatg aattgacaaa cttagaagca
    11101 gttgtaaaag caatccatcc aacgatcatg atcggtactt ctacccaacc tggagcattc
    11161 actgaagaaa tcgtgaaaga aatggcggct cataccccac gtccaatcat catgcctttg
    11221 tctaatccaa caaaattggc agaagcaaaa gcaaaagatt tgattgaatg gacggacgga
    11281 aaagctttag ttggaacagg tatccctgct gctgatgtgg aatataacgg tgtaacttac
    11341 caaatcggtc aagcaaataa tgcgttgatg tacccaggtt tagggcttgg tttgattgct
    11401 tcaacagcta cacgagtaaa tgctgaaatc atttctcaag ctagtcgagc attaggcgga
    11461 atcgttgatg taacaaaacc aggagcagct atcttgcctc cagtagcaaa aatcactgaa
    11521 ttctctcaaa cgattgctga aactgtcgca aaatcagttg ttgcacaaaa attgaatcgt
    11581 gaagaaatca cggatatcaa agaagctgtt gaatctgcta aatgggttcc tgaatataaa
    11641 tctttagaag attaagattg ttatagtaaa agaacataca gaaactttgt atgacgtcta
    11701 aaaagaaaaa agcgactgcg gcaaaacagc agccagccaa ctactgctct tttgccgctt
    11761 ctctttggct tttttcatac gatacagaat aagaaaggat ctgtaaaaat ggcacaaaaa
    11821 acaacaaccg caactgcagt tgaggaaaag aaaaaagaag ggttttggtc aacaaaaatt
    11881 accggcgtaa gtttgcccct ttatttgatc atggtccttg tcctgatcat tgcaatggct
    11941 ttaggaaaat taccgacgaa tatgattggc ccaatcgctg ttttggtcat tttcggtaat
    12001 ttatttcatt ttatcggaac aaaaatccct atcgtcaaaa gttatttagg tggtggctcc
    12061 gtttttgcga tctttgcctc tgccgcgatt gctacatttg ggatcttacc tgagtatgta
    12121 gtaaaatcca cagaaaactt tgtcagcaat atgggctttt tagactttta tattgctgcc
    12181 ttgattactg gtagcatcct tggtatgaac cgtaatctat tgatgaaagc ttctgttcgt
    12241 tttattcccg ttgcacttat ttcaatggtc gtctcctttt ttgccgtagg actcgtaggt
    12301 atgcttatcg gaaatggttt tgcaaattcc gttttatata tctcattgcc cgcaatggct
    12361 ggtggtgttg gagccggagc tgtaccttta tcaacgattt atgcaaatgt tttaggtacg
    12421 gacgcttctt ctatcatttc ccgcctgatt cctgcgacag caatgacaaa tgtcctagct
    12481 atcattggtg cagcgttagt tgctcgtgcg ggacaatcaa tgccaaaatt caacggtaat
    12541 ggtaaattaa tggaaaaagg cgaccttggc gatggaaaac ctagaaatgt aaaaccttcc
    12601 attccacaat taggtgtcgg tttgatggta tcattgactt tcttcatctt aggacaaatc
    12661 tgtaactatt ttgttcctaa agtccatgct tacgcattca tgatcattat cgttgttatc
    12721 tgtaaaatcg caaacctatt accagaatat tacgaagatg cagctatcat gttcaacaac
    12781 ttgattgtca aaaacttgac agcagctgtt cttgccggta tcggtattgc gttattgaat
    12841 ctaaatgtat tggcttctgc tttgacatgg caatttgttg tcttatgttt gacaagtgtt
    12901 atcgcgattt cagtagtttc aggatttgtc ggtcgtttgt ttggtttata tccaatcgaa
    12961 tcaactatta ctgctggctt atgcaacaac tcgatgggtg gtacaggaaa tgtagctgtc
    13021 ttatctgcag ctaatcggat ggaacttatt gcttttgctc aaatgggcaa tcggcttggt
    13081 ggtgccatcg ttttgatcat ctcaggtttc ttgatgcaat tactttctta gtcaaaaaaa
    13141 atacctgatc atcaagtatc ggcttgatga ccaggtattt tttgataaaa atttacagga
    13201 ttttcaaaaa tcttttttgc attttatcgg aatgataacc gatgcgttca taaaatcggt
    13261 gtgcttctac tctgtattct gcagaattaa gtcttattgc tgatattcct cgtcttttcg
    13321 cttcttctac gccagtcatc aaggcctttc cgattcctct tttttcagca tgtttagaca
    13381 cagcaagtgc cataatattg aacatcggat cgctgaaaat cgattcatag gactccgctt
    13441 gtacatagcc agctgctttt ttagaagtag gatcttcata gaccaagaaa aaattttttt
    13501 ctggctcctt caacagtttc ttcatttgtc tcaccgtaac ggttaatggt acatcatatc
    13561 ctaattgttc cgcattgatt tctcgtagtg cttctgcatc tcctaaacgc atttcccgca
    13621 acatccaaat caccagccaa tttttatttt tttaaatgaa tttttttatc acagtatact
    13681 ccttttttca tttagaacaa tatgtttcag tgattttgac ttgcgttagt ctagattctc
    13741 ctaatgtttg taagatgaca aaggcattgt aacaattgac caaaagaacc acgaggacaa
    13801 ttacttgaaa aataaaggtc actttttttg gagacaaaac cctgctaaat cttgctcccc
    13861 acaaacctcc aataacagca gcggggataa cataaaacaa catagataaa tcaaagcgca
    13921 taaagctact tgttaaagca atcgttacca attttgccag ttgagaaaag aaaatcgtag
    13981 atagagaata tagtgttgct tcttttattg gcaatgcaaa catcagcatc aacaaagaaa
    14041 cattgactgg tccaccacca atacctaaaa aactagctaa aaagcccaga acccccccac
    14101 atataaggta ccaagtccat gacgtcaaat gaaattttgg ctgatgatgc ttggtgtaga
    14161 agaacgcaaa aaccaaggtc aataccgtca aaaaaatttg gatcagctgc acatgcttct
    14221 cgttttcaaa taactgtaaa aaaacttcga atacaatgtt gccgactact cctccaacaa
    14281 cagctccact agatacccaa ccgacaatct gccaattcag tgatttcccg cttgatactt
    14341 gtcgcacagt cgaaacaatg gacatcgtga ataccgcaac tgttgagtaa aaagaaatcc
    14401 ctgcaacaga atgggcgccg ataagatcaa gaatcggttt gatcaacacg cctccaccca
    14461 tcccggaaat agcgccaact gtgttcgcca gaacgatcac cacaaaataa atcagaccaa
    14521 tcatcttgta tttcctttct cttgtttata tcttttagcg attgttactc ttttctttta
    14581 ttatacagta aaagaaacaa acgaggcttc gaaagctttt ctacttttta aggaaattta
    14641 gtgctaaacg aaaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa
    14701 acaaaaatag ctcctcataa ttttcgtttg gttgaactta ttaccgcaga tgcaagcttt
    14761 tgaacaccgt ttaagaaaga ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca
    14821 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttggc
    14881 aggagaacac cgtttaagga agagactgtg aagtcaacgt tttgacctga ttattagaga
    14941 aacaaatggc aaaatagttc ttcatatttc gccgtttttc gagcgaatgc cgaggggcag
    15001 ctgattgaac atcgtttatt tggaaaaagg gattgtgaca agacgtttgt cacaatccct
    15061 ttttttatat cttattaatt cattcttttg ttctattctt cttcttccgc catgaatgag
    15121 aaacccatat caaagaacta taaaggttat caaaacagcg tttatgacta aatgaattag
    15181 agttatctat cactattttt caagcgattt aaaccaaatg gcatacaata tggcatacaa
    15241 aaacttcaat aagccgtggg ggtttttaaa tttcttcttg ttctttaaaa tcaatgagtg
    15301 gagctacata atttgaatcc actttttcaa acgtcacttt ttgcatcgac gaatgttttc
    15361 tttcccaaga taacgtaaat agatttgact ctggacttat aaagtctact tcaccttcta
    15421 gtattacttt cttatgc
//
LOCUS       DAYYHX010000052        15306 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000052 DAYYHX010000000
VERSION     DAYYHX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.052"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            79..807
                     /gene="sfsA"
                     /locus_tag="ACHA6Y_001214"
     CDS             79..807
                     /gene="sfsA"
                     /locus_tag="ACHA6Y_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM8987323.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            823..1836
                     /locus_tag="ACHA6Y_001215"
     CDS             823..1836
                     /locus_tag="ACHA6Y_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8987324.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            1836..3113
                     /locus_tag="ACHA6Y_001216"
     CDS             1836..3113
                     /locus_tag="ACHA6Y_001216"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8987325.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3176..5878
                     /locus_tag="ACHA6Y_001217"
     CDS             3176..5878
                     /locus_tag="ACHA6Y_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8987326.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6030..6347
                     /locus_tag="ACHA6Y_001218"
     CDS             6030..6347
                     /locus_tag="ACHA6Y_001218"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8987327.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6377..6697
                     /locus_tag="ACHA6Y_001219"
     CDS             6377..6697
                     /locus_tag="ACHA6Y_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8987328.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            6805..8265
                     /locus_tag="ACHA6Y_001220"
     CDS             6805..8265
                     /locus_tag="ACHA6Y_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8987329.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8333..8554
                     /locus_tag="ACHA6Y_001221"
     CDS             8333..8554
                     /locus_tag="ACHA6Y_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987330.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            8585..8767
                     /locus_tag="ACHA6Y_001222"
     CDS             8585..8767
                     /locus_tag="ACHA6Y_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM8987331.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            8767..9180
                     /locus_tag="ACHA6Y_001223"
     CDS             8767..9180
                     /locus_tag="ACHA6Y_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM8987332.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9303..10484
                     /locus_tag="ACHA6Y_001224"
     CDS             9303..10484
                     /locus_tag="ACHA6Y_001224"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8987333.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(10555..10719)
                     /locus_tag="ACHA6Y_001225"
     CDS             complement(10555..10719)
                     /locus_tag="ACHA6Y_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987334.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            10873..10946
                     /locus_tag="ACHA6Y_001226"
     tRNA            10873..10946
                     /locus_tag="ACHA6Y_001226"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10907..10909,aa:Arg,seq:cct)
     gene            complement(11015..12154)
                     /locus_tag="ACHA6Y_001227"
     CDS             complement(11015..12154)
                     /locus_tag="ACHA6Y_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8987335.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            12326..12460
                     /locus_tag="ACHA6Y_001228"
     CDS             12326..12460
                     /locus_tag="ACHA6Y_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987336.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(12453..13088)
                     /locus_tag="ACHA6Y_001229"
     CDS             complement(12453..13088)
                     /locus_tag="ACHA6Y_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987337.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(13201..13836)
                     /locus_tag="ACHA6Y_001230"
     CDS             complement(13201..13836)
                     /locus_tag="ACHA6Y_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFM8987338.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(13870..14331)
                     /locus_tag="ACHA6Y_001231"
     CDS             complement(13870..14331)
                     /locus_tag="ACHA6Y_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFM8987339.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(14461..14892)
                     /locus_tag="ACHA6Y_001232"
     CDS             complement(14461..14892)
                     /locus_tag="ACHA6Y_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8987340.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(14910..15230)
                     /locus_tag="ACHA6Y_001233"
     CDS             complement(14910..15230)
                     /locus_tag="ACHA6Y_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8987341.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT         4881 a         2583 c         2971 g         4871 t
ORIGIN      
        1 taagactttc gactggtttt tttactgttt ttctacaaat tatgatagga tacatagtag
       61 gaagaaaggt gacataattt gaagtatcag caagtggaaa tagctttttt tattgaccgc
      121 ccgaatcggt ttatagcaca ttgtatgaaa aaagacggag aaataattat tgcgcatgtc
      181 aaaaatacag gtagaggaaa agaggtcctg ataccaggcg ctgaagtggc tattgtattt
      241 gctcctggac ctaaacggaa aaccgcctat gatctgattg ctgtaaaaaa gaagggtgac
      301 tggtttaata tcgatagtca attgcctaat cggctggcaa ttgatggtat tttagacggc
      361 acgatccttt taccaggatt aactggtcca tttacggtat acaagcgtga agtaacttat
      421 cttcactcca aatttgatat ttatttagag acggtggtcg gacaaaaagc atttgttgaa
      481 gtcaaaggta tgacacttga aaataaagcg attggtgctt ttcctgatgc gcccacactc
      541 agaggattga aacatatagg agaattaacg tatgccgcac aagatggtta tgccgcttat
      601 gtcttgttta ttgctcagtt cgaacatctt catctggcaa cgatccatga agaaatgcaa
      661 ccagcactag ctgatatggt tcgacatgcg cagcaaagcg gagttcaaat cctcgcctat
      721 aattgtcaag tatcggcaga tgaggtaact gtcaagcagg ccattccatt tgatgtagat
      781 atcccttttg aagatcctat acaatagaaa gaaggtaaaa taatgatcaa cttaggtatt
      841 atcggaacaa attggattac tcatcaattt gtccaagcag ctttaagcac aaaaaaatat
      901 gaattgacag cggtatattc tagacatttg gaaaaagcac aggaatttgg ttctcattat
      961 gaaggggata tcgaattcgc aacagatttg aagacttttt tcaatattac tcatatggac
     1021 accatttata ttgcttctcc gaatagtctt cactttagtc aagctaagca agcaattttg
     1081 gcaggaaaaa atgtgatagt tgaaaagcca gctttttcga cgccaaaaga aatggatgaa
     1141 atcatccatt tggcaaatga acacaatgtg tatttttttg aagcagcaag aaatatccat
     1201 gaatcaggtt ttaaaaagat tgccgattac ttaccattaa aagacgagat aatcggtgca
     1261 aatttcagct atatgaaata ttcttcgcgc tatgaccaag tattagatgg agaagaaccg
     1321 aatatcttct cgactcattt ctcaggtggt gcattgatgg atttaggtgt ttaccttgta
     1381 tatgcagctg ttgcttggtt tggaatgcca aaagaagtcc attatttccc aagaaagatt
     1441 tctactggtg tagatggtct tggatggggg atcttacgtt atgagaattt tgatgtatcg
     1501 atccagccag gtaaaatcgg tgattcttat ttaccgtcag agatttattt tgactcagga
     1561 acgctagtga tgaatggagt caatgcaatt gaacaagcac aatttcatga tcgcgcgcat
     1621 aaagaaatca ttgatttggc ggtaacagct gcagaaaatc caatggcaga tgaagcagag
     1681 gattttgcca atgtcatggc tcatccgaaa gatccagctt ggggattgtt gtatgaagag
     1741 tggatcgagc tagctagaaa tgtgaaccaa gtgatttatg atttaagaaa aaatggcggc
     1801 atcaaattcg atgcagataa tgaggaagat ttttaatgaa cgaatttgta caagtatttc
     1861 ccgaaaattg gcagaaaaaa tatgaggata aagggtttat ctcaccttct cttattcaac
     1921 gagcagtttt tcagccacta agtaataaac aaagtatagt agcggtttct cctacaggtt
     1981 caggtaaaac actggcttat ctttggcctc tactactaaa cgtggaacca ggtgaagcaa
     2041 gtgcgttggt catttttgct tcttctcaag aactagctat tcaagtagca gatgttgcac
     2101 gtgaatgggg aaaagacaaa gagttgaaag tccaatcttt ggtgggcgga gccaacgtca
     2161 aacgtcagat tgagggtttg aaggaaaaac cagaaatctt gattggtaca ccaggaagaa
     2221 tattggaatt gatgaaagca aaaaaaataa aagcacatca agtgaaaaca atggtatttg
     2281 atgaagcaga ccagctattt gacgctggga acagccaaat cattgatcaa attcttcatc
     2341 aagctccaac agagtatcag ctagcttttt tttcagcgac agcagatcgt tcccttgaaa
     2401 gcattgaaaa aatcactggg aaaacgtttg agacaattga cgtgacagca gaggacgatt
     2461 ctcgaaaagg actgagacat tactttttac gtgtaccgat acgaaagaaa gatgactact
     2521 tgagacgtct cacgcatata gaagattttc atggtctgat tttctttaat caactgactg
     2581 aattaggcgt aatggaagaa aaattgctgt atagaggtgt accggtagga agtctggcct
     2641 ctgatcagaa caagctactc cgaaaagcag cgctagaaca attcaaaaag aaaatggtca
     2701 gagcactttt gaccacagat gtggcggcta gaggtttaga tattacaggt cttccttatg
     2761 tagtgaatgc agaagttccg ttaagtgaag aagcttattt gcatcgttcg ggaagaactg
     2821 gtcgaatggg taatgaagga atagtaatta cactcgtaca agataataat ctaagagatt
     2881 taaaacggct tttacgtcca tcaaatataa cacttgaaga gattttttta tacggaggta
     2941 agctgcaaac tgagcagcca accaaagaag atgcagctcc tgcagtaaat aaaaagtatg
     3001 cgaaaacttt ttctggaaag gagcagaacc cagaatataa caaagaaaaa ataagaaaaa
     3061 aagttaaaaa taaatcaaaa aaagagagaa ataaaggagc aaggcgcaaa taagcgcctt
     3121 tttatgctat gattatgaaa acgggaacaa aattggttgc cagaggaggg gttatgtgaa
     3181 agaacgtcaa gaagagatct tgcagctatt gacagaaaac caacaaggat tgacagcaag
     3241 tgatgtagct gagcgcttaa ctatcgatcg aagcaatgca agccgatatt taagtgaact
     3301 atacaaggcc catcatattg taaaaaccgc tggccgacca gtcgtttact ctttgccaac
     3361 ggaaaagtca aaatctgatg aagtgcatgt cgactcttct acgcaagtga cttttgaaac
     3421 actcgttgga gaaaatgatt ctttaaaagt cagcatacag caggcaaaag ctgcgattct
     3481 ttatccaccg cgggggttgc atacgattat ttttggagaa actggtacag ggaagtcgat
     3541 gtttgctgaa tgtatgtatc attttgcaat cgattcagag atgctttctg cagatgcgcc
     3601 atttgtttct ttcaattgtg cggattatgc tcaaaatccg caactgctct ttggacacat
     3661 ctttgggatc aaaaaagggg cttacacagg agcagctcaa gatagtcccg gtctaatagc
     3721 caaagcagac ggcggtatat tgtttttaga tgagatccac cgtttaccgc cggaagggca
     3781 ggaaatgctg tttactttca ttgataaagg tatttaccgt ccgcttggag aaagtgcaca
     3841 agtacatgaa gcatctgttc agatcattgg ggcgacgact gaatcttcag aaagtttctt
     3901 atctacgttc aatcgacgga ttcctatggc aatcaccttg ccgaatcttg cttcacgttc
     3961 actggatgag cgttatgaaa tcatctctct ttttatcaaa caagaagcga atcgtttgaa
     4021 tcaacgtatc gatatcgaaa aagaagcgat tcttgccttc atgctttatg atgcggaagg
     4081 aaatatcggt caagtgaaac gagacttgaa actagtctgt gcaaaatctt ttttacatta
     4141 ccgtactcat catgagaaaa agctaatcat tcgaaaagaa gaactttccc ttcaagtaca
     4201 aaaaggatta ctgaaaatca aggaaatacc ggaacgtctc gaccgattca tggacagcaa
     4261 aagtcagtat ttgacatttg aaccaggatt tgctgatgtt gtctggtcac aagatccaga
     4321 acgaaacatg caggtctata acgatattga ggaaaaagtc acttctcttt ccgaaaccgg
     4381 tgtggagaac attgatctag agaccctcat ttcaaaagat gtggatgctt atttccagac
     4441 atatgtcaaa gaattgacta aaagttcgat tcccaaagat cttttgccag atgaaatctg
     4501 gcggttaacc aatcaattgt atgatgtggc tgaaaaacgg ttagaccgta tttataatga
     4561 aaaagctcgt tttgcttttg ctttgcatct gcaaagtaca ttggatcgtg tgagagaagg
     4621 tcatatgatc gtccacccag atttgaataa cgtacggaaa aattttaaga gggaattcca
     4681 ggtagcaatc gatctttcaa cgatcattga agaagaagaa caagttgaga ttccttttga
     4741 tgaaatcggc tttattagta tgtttctttc tatcaatcta ggagaaagtg agcctgttaa
     4801 agaaaacaaa gtagatgttc tcatattgat gcacggcaga cagactgctt caagcatgtt
     4861 ggagatggca caagaactat taggaacaaa agtagggaaa gcttttaata tgccactggt
     4921 tatggaagtt caaacaatgt atgaagaagt gttacaatat gtcaaaaact atcaagagca
     4981 tttgacgaat ggcctactct tgttaacaga tatgggatca ttaaacacct tcagtgattt
     5041 gatttatgaa gagactggca ttcggacgaa agctatcacg atgacaagta ccatgattgt
     5101 gatagaagcg atacggatgg caagtgttgg aagaagctta gaagatattt atcaaaatat
     5161 ccagctttcc tttgaatcga tcgtacgtga tcagttcaaa gcggaaaagt ctgaaacttt
     5221 acagcgtaaa aaagccgtaa tcgtgacatg ctttactggt gaaggagtag ctgccaaatt
     5281 atatcagcgt attatgccag tgattaatca aaataaggta gaactgatcc agatgcagtt
     5341 tttagaacgt gaatcattca aaaaacacat cgatgcctta ttggaagaat atgagatcaa
     5401 agcaattgcg ggaacagttg atgttgatta tcaaaatatc ccattttttt cagcctatga
     5461 cgtttttgat gatgaaaaac taaatatttt aaagcgtata gctgatgatg agatcccgac
     5521 agctaaaatt gtcgattcac ttgctggatc attaagcgat gtcttttcta ttgatgaact
     5581 gatccgttca ttgcaaaaga cagttcagca gatccagacc gatttgtcga ttatcgtgga
     5641 acctggagta gatactggaa tcgttatcca tttagccttc atgttagacg gattgatcaa
     5701 acaagaaagc catcggcaat ttgagaaact gtctattttc tcaaaaattt atcgtttgga
     5761 aatggatatc gttcgggcaa acttgatggc actagaacga aaatatcagg taaaaattcc
     5821 agaagatgat atcgcatttc ttacacaaat gtttcttgaa aataaaataa aaaaatgaac
     5881 acactaatca tactgtttac acactaaaca tctgtgtgcg cactataaaa aagagctaaa
     5941 aagcatgaaa acgctatttc agctcttttt tttattggca tgcttcttgc gttatataaa
     6001 agtgtaaaaa aataaaagga gtgttaaaaa tggcaaaaaa aacaatcatg ttagtttgtt
     6061 cagcagggat gagtacgagt ttattggtca caaaaatgca aaaagcagca gaagaaaaag
     6121 ggatggaagc cgatattttt gcggtctctg cctcagatgc agataataat ttagaatcta
     6181 agaatgtcga tgtattatta ttaggtcctc aagtacgttt catgaaagct caatttgaac
     6241 aaaaacttgc gcctaaaggt attccattag atgtcatcaa tatgcaagac tatggcatga
     6301 tgaacggaga aaaagttctt gcacaagcag aaaatctaat gaaataaatc aagtatgcag
     6361 ttgggggtat aacaacgtgg aagatcaaca aaatctagaa gccatcatgg ggttaatcat
     6421 gtttggcggt aatgcaaaga gcgatgcaat ggaagcgatt gcggctgcta aaatcggtga
     6481 ttttgagtta gcggatagga aaatcgctga tgcagaagaa tcacttatac aagcgcatca
     6541 tgcacaaaca gacatgttga cacaagaagc acaaggaaat catatgcaag tgacattatt
     6601 gactgttcac agtcaagatc acttaatgac atctattgcg tttactgatt tagcaaaaga
     6661 aatcatcgaa ctttatcgtc gcgtatacag cgaataaata caacttcttt tttgaacgga
     6721 agaagaaaaa tttttgaacg gattttttat tttgttataa ggagaactct ctttatataa
     6781 aaaacttata ggaggaagta aaaaatgaac ggactatcaa gttggatgga gaaatacatc
     6841 ctgccggttg ccgcaaaaat cggagcgcaa aaacatttag ttgctttgcg tgatgctttt
     6901 atcggcatac tgcccgcaac aatggcggga tcagtcgctg taatgatcaa tgcaattatt
     6961 cgtgatttac cgcaacagtt taacagtacc tatgctgaca gtttagcagc agacaaagga
     7021 atctttgccg tattgaatgt catcatcgga atcaatggtt ttgtctggaa cggtacacta
     7081 gcaatcgccg gattgatctt tgccttttct tgggggtata atcttgcccg agcttatgga
     7141 gtaaatgatc tagctggggg gatcgttggt ttagctacct tgattcaagg tatttccttt
     7201 ggtccagcat tatcagcagc attagatgtg aatgttcctt caaatgtcgc agatgcaatc
     7261 aatagttctg agattggtgc aacgattgca gatggcacac tgtcagttgg attatggggt
     7321 tggctaaaac tagatcattt gaatggaaac gcttatttta cagttatgat catgggagct
     7381 atttcagtca ttattttttg taaattgatg cttgccaaca ttacgatcaa aatgcctgat
     7441 tcagtaccac cagcagtatc taaagcattt gcagccattt taccagcgac gatcgcttta
     7501 tacgtcattg ctattatcaa ttatatcgta ggtcgtgtag ctgatgggca actgatcatt
     7561 gatttggtgc aaaaatatat tgcagaacca ttcctaggat tatctcaagg gttgggagca
     7621 gtattgatcg tcacagtatt tgttcaagta ttctggttct ttggtattca tggatcaaat
     7681 gtattggcac cagtgctaga gggaatctgg ggacaagcac aactaatcaa tattgatatt
     7741 ttccaaaaag gttataacgg aaaaactggt acaccagccg tgttagatgc aatcgaagat
     7801 ggaaaagctt atatgtgggt ccgcggatcg ttcgatgctt ttgcatggtt tggcggatca
     7861 ggcggaacga tcgtcttact gatagctatc ttgattttct ctaaacgtgc agattatcta
     7921 acagtagcta aattatccat tgggcctggt atcttcaata tcaacgaacc agtgatgttt
     7981 ggtctgccaa tcgtattgaa tgcaatcatg tttatcccat tcttgattgc tccagtagta
     8041 gcaacagcaa ttggatggac agctacttat cttggcctag tcgctccggt atcgcagcaa
     8101 gtcacatggg tagtaccgcc gttcttattg tcattcttag caacaggagc ggattggcgg
     8161 gcaccgatcg taacattagt atgtatggtc gtgactttct tgatttgggc accatttgtc
     8221 atctctgcaa acaaaatgga ccctgccgca ggcgaacaac aataagatga acgtgaggag
     8281 cgggacgctt taagtttcgt tcctcataat gaattttcga ggaggaactg aaatgattac
     8341 aattgccagt gcaatgcaac aagatgaaat caaagaattg ttagaaggat acaatgaaga
     8401 aggaatgact tttacattta aagaaaaaca aggaatcaag ctgttctttg aaacaagtgc
     8461 agaagatccg gaagttgcgg ctaaaaaagc aaaggaacta atcaaagcac agccttgggg
     8521 aacagtcctt tattttcaat caacagctgt ttagaaaatg gtttaaaaag ataggatggt
     8581 tgagatgata aaaaacggtt tatttttatg tagtattggt tctttgatcg tgttgtatag
     8641 tttgaatcca cattcgcaaa ggtatgattt gtatagtatg gtcacaggtc tttttctaac
     8701 aggattagga atctatttct tcatgaaggg gaaaaaaaga gaagaagcaa aggagaaaaa
     8761 taactaatgg aaatcacgaa aaaattaaat attccagcat cgttttttta cgataaagtt
     8821 atggactctg tccttttcga tgtacgtaaa gcgaccggca aatcagtaac tagaaaacag
     8881 ctgaaaaatt tggagtacgt caaacaattc tcgcaaaata accgggctcg cataaaaatc
     8941 gaagaagtca ttgaaaacca gtcttataag tttcgtacat cgacaacgaa gaatgaattc
     9001 gtcgttcatt atcaaatcaa accattggac gattcgacct gtgaggttca ttacacagaa
     9061 aaaatggagt cttatggcat gcttcaaaag ttgaatgata tgcttttagg aacaatcttg
     9121 atgtatttta aacggagacg tttcaaaaaa atgctagata tgatcgaagc aacgtattaa
     9181 atcaatgaaa catggtacta tttagttatt gaaaaatgaa gtaagctaga aggaattttt
     9241 cataggaaga tagatctttc ctgcgaatcc tcactagctt ttttgtagga aggggcagca
     9301 aaatgacaaa caaagtaaga gagctaggtg tatcttcgat tgaggaaatg tttgatttag
     9361 ccacttatgc atttaatggg gcagatacac ctgaaaggcg agaacgtttt cgtacacttg
     9421 cggagaattc ttggaattac ggattttttg atgaggagga tcggctaaca agtcaagtca
     9481 tggcgacccc ttttccagtg aatttttacg gacaagagta cctaatggcc ggcattggct
     9541 atgttgcttc ttatccagaa gcaaggggac aaggcgggat caatcagatc atggaaaaga
     9601 tccttgaaga ttgccgtgat cgtaaagtaa gcttatctta tctggcgcca ttctcctatc
     9661 ccttctatcg gcgctatggc tatgaacaat catttgataa aatcagttat caactaaaca
     9721 gtcgtgatgt gccgtttatc aaaaaaaaat ctggaaaaat aaagcgtatg gattttgaag
     9781 atgcaaaagc tgcaatcaga agaatttatg aacagatgcc ggaaaaccaa cgagctggtc
     9841 tgaagcgcga agactggtgg tatacgtata aattcaagca aaaaggcaat taccagtttg
     9901 ctttgcattt tgatgataat gaggaagcaa cgggatatat cgtttatcag cttgcaacgt
     9961 cttcctttat tattgcggag tggggatttt tggatcatga tgctttttgc agccttgttc
    10021 gcttcgtaag ttctcacaat ggtgcttttg aaacatttga atattcatgc ggctacggcg
    10081 gtaatgatca aaattatcta ttagaaaatc catttgcttc tgtaatgatc acaccttata
    10141 tgatggcgag gatcgtagat atttctttgt ttttggaaaa ttatccattt aaaaagagac
    10201 aagctgcatt tgccctacaa atagaagaag atcattatgc gaaatggaat gaaggaatcg
    10261 tggaagtaac aatcgaaaat ggaaaaaatc atctctcaaa agtagaacaa acaaaactcc
    10321 caatcgttcg aggaagtatc caacatatca cgcagctatt attagggtat cgtaaagtgg
    10381 aagagttggt attccaagaa agaatacagg tgacaaaaga atatgaagaa acgttaagct
    10441 caatcttgcc acagcaacag ccaattctta atgactattt ttgataaaaa acgggtgatg
    10501 acaatatttt tgtcaccacc cttttgtttt tcatttggca tttgaaggaa aatgttaaag
    10561 taaatcctct tttttcgtaa atttgaattt tttgttttct tttgttttag ggttttcttt
    10621 tttgtggtct tggattgctt ctttcgtaat cttttcccga ttttttttag atgaatctaa
    10681 gcgttctgcg tatacttttt cttttggctt atcattcatt ggaaaggacc tccctacatt
    10741 atttcattct acctctagtt tatcatctgt taaggactag tggaaacgtt ttgcctaact
    10801 tataagctga tttttttagc agataagctt gcaagtaaaa aacaaacaag ctacaattga
    10861 tttattgaaa ttgttcctgt agctcagtag gatagagcaa tcgcctccta agcgataggt
    10921 cgctggttca attccagtca ggaacgtata aataacccct gtatcctttg cggtacaggg
    10981 gtttgttttg tatttgtgcc aaaaatgtgc caaactagtt atcattttta tataaactat
    11041 cgaacacagt attgattttt tctttatcct gatcttcaag ctctcgaaca atatgaagat
    11101 aagtagacat tgtagtttct aaactgctat gtcctaaacg tttcgatacg ctaagtatat
    11161 ttactccttg ataaagtaaa atagatgcat gtgtatgcct tagtccatgc aaagtaaatt
    11221 gcttttcaat ccctaattct tttaattttt tgcgcaaata tttgctgacg gcgtttgatg
    11281 aaactaatcc attttttaag ttgaaaaaaa caaaattatg agggtttttg atttcaaaat
    11341 tctcgaataa ttctttttga ttgattttaa acttttttaa gagatcaatc gtatgtttat
    11401 caatggaaat tttcctattt gaagtttcgt tttttgtttt tccccattca tttaatttat
    11461 aattccatgt tttattaata gtgatgattt gatcttcgaa atcgatatct tcccatgtta
    11521 atcctagaag ttcagcaaat cgcattccag tggcaccagc aactaacacg agcataggag
    11581 aagagtactg cgcagaaagg ttttcttcta cgagtttcat taaagattta aattgatcat
    11641 aatctaaata tttatcttct tttggcttca atgaatcttt tcctttgatt actgcttttc
    11701 tagttggatc aaacggaatt aatccttctt ctacggcatc tttcaacgac gctctaatat
    11761 ggttattaaa cttaataacg gtagatttta catggtcttt agcatactta tttaaaaagc
    11821 gttgatatcc cactctatct aaatcagaaa tcaataccgc tggcatatat ttctttatgt
    11881 tcattaacgt atcttcgtat tttctataag tgataggaga tactgttcct tctttataaa
    11941 gttgcatcca atcctcaaag taatctgata ataatagatt tttgcgatcc cctttaaggc
    12001 ctttgttcaa gttatattct aattcgttgg cggcatcttt tgcttctcct tttgtcttaa
    12061 atcctgattt tcttatcttc gtgtattttc cgtcatcttt tttgtatgaa atttcgtatt
    12121 gccaactatt tccacgtttg actaatcttg ccataattga atcagctctc tttctctgat
    12181 acaataggaa ctataaagaa gcccattgtt tgggttttat tattttttgc acatcctttt
    12241 tgcttgccgg cgcaaggatg tgctatttgt cgttttaatg gtataattgg atgtgtatta
    12301 gatcatccaa tcagaggtgt ttattatgat taaagtgatt tttaaaagaa taactgaact
    12361 tccagagttt agattatttg ttgattattg gtatgtctgg ttttttattg ttgcattgct
    12421 gataggtcta gatgtcatat tagaaagaaa acttaagtaa gagttgagtt aataaattga
    12481 attcctaaat gagatatttt gtagcccata ttttgtttgc cgacatcacc taatactatg
    12541 gagctaatcg ttttttctaa cagaccaaaa tcagataatc tatttaagct accatctatt
    12601 agctcttgtg aatagccaga catgctgata tttccagtga aattttcgtt tccacgactc
    12661 attaagcatg ctaacaattg aatctcaatc gatgttaatt gattcagtac aagaagataa
    12721 tactcctcgt tatcccatga agcgttgtta attcgaagta aacaattctt ataaagattt
    12781 ttatacatat ctattttatt ttgtgccttt gatttttcta cctcgtcatt tatcccttcg
    12841 aatattccaa tgagttggtc cttgttctct aagttgaaaa catcttctgg aatttgatcg
    12901 cgaatgtttt ctaatgattc gtttaaattt ttgtagaatt tctcgatgcg tttgaaacgc
    12961 ttctcgtttt tagacccaaa ataaagtgac tgcacaacgc caccaatata aggaattgct
    13021 ccaataccgc cttcaactat taattcaaga tattcttttt tggttagttt ttcattgcta
    13081 ttatccatag ttacctccta ttttgtcatt tcctttacaa gcattgtgac tcctgatggc
    13141 actcttttgt ataggtttta ttattgtgca caatctaact cttagtgggg cgtggttcat
    13201 ttatttttta cttactttac tattaatcat atcccattga gcataggatt tttctatgtt
    13261 cgatcctctt gaactaattt ctcctgtaaa agttacttta tcgccctttg taaaggtatc
    13321 agcattgttt aagtccttcg caaaaattac atatgaaact ttaggtgttg cagaaatatc
    13381 attccaactt ttatcatcgt actctttgtc tgctataatg gctattctag tagtcattga
    13441 ttcgataaca gttccttcaa aagtatatgt tttaccacta atatatttag taaaagctgt
    13501 attttgctca gtagctggag taatagaata ataactatct aagaaagcac cccagtcatg
    13561 attatttgtc tctaaaaatt cttcaaattg agttaggctt ttattgctgc tgttagatgt
    13621 agtagtttga gtatcagatg atgagctttc ctgtttgtta ttgtcatttg cctcgtttga
    13681 tgaggaaagc gagaaaccta cggtagtaaa aataatcccg ataataagga gaatcatagg
    13741 tgttttctta tttgtttttt tgataaatct tacaattaac atgattaaac ctataataaa
    13801 taaaacaata ccagtaaaac caaatatacc gtccattaat agatcctctt tctcttttga
    13861 tattgaatat tataaaccaa gcaattcttt tttcttaaaa tcgaattctt cttgattaat
    13921 aatcccttca tctaacaact ctttatactt tttaatttca tctgcacttg aaatttgtgt
    13981 ttctttggaa ttattatcta atatagattg ttgcttctct atatattttt ttaaattttc
    14041 catttcattc caatattttt ttgtaaacat aacggtgttt tcatctttcg tggccgctaa
    14101 tatacctccg cgactttcgt ttccgcctag caaggtaaat tgtatataac cattactcat
    14161 acctggtttt tttaattgta cggctgatat atttttaaag gggatagttt tttctccttt
    14221 aataccttga ttaactaaat ttataaatcc ttttctaata attgaaatag aatcatcagt
    14281 tactttaatc aaggtttttc ctggagattt tattaaaatc tctttttcca ttttttcttc
    14341 ctcatttctg tgatatgatt ttttttgtag aagctcatat atgaggaaag agcccgtgtt
    14401 gcagcacggg cttttttaat gttttgctga aatcggtttt tttaataatt cttgatgtat
    14461 tcatacatat tgccttgcgt aagaatattt tttcttaaaa tagcattggc agacagcata
    14521 acaagtgtat ctgagcttat tctgaataga ataatactcc ataaattttt cgagattaaa
    14581 ctgagattca tcagtcagtt cgttctcaat atagatattt aataaaatta gaatagctat
    14641 tttatctgct tctgtttcaa attttgagtg aaaagttgta gaagtatcgt acaaaactga
    14701 aaattcaaaa tgagaggcgc tgaaatgtgc aagttcatga gataaatgaa aggcttctgc
    14761 agtttcaccg tatagatttt cattcaaaaa aatgattctg ggttttggat agtagaaacc
    14821 aggctctttc atctccatat aaactacttt caaattgtat tcgctcaaca tttctttcaa
    14881 tttcaaatac atacaaacca tcactccaac tattcatttt cctctaaagc tttagcaatt
    14941 gcaatcgctt tacgcattgt ctccttagat atttcttttc catcaaaaga aaaaacagta
    15001 tcgtcttctg ataaatccac atgtttaggg gtttctcttt cttctctacc tagaaggtag
    15061 tctacagaga catcgaaata gtcagcaatc cgacttaatt catctgaatt aggagtatta
    15121 tttttccact ttgctagata accgttagaa tatccaaaat taatttctaa ctgccgtata
    15181 gatatctttt tcctcttcgc caagtctttt attatttcat aggtattcat cgataaaaca
    15241 acctttctaa atgtttacaa aaaaagttta gaaaaatacg cagaaataat ttgactaact
    15301 ctgagt
//
LOCUS       DAYYHX010000053        15229 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000053 DAYYHX010000000
VERSION     DAYYHX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.053"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACHA6Y_001234"
     CDS             278..787
                     /locus_tag="ACHA6Y_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8987342.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACHA6Y_001235"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACHA6Y_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8987343.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACHA6Y_001236"
     CDS             2322..3302
                     /locus_tag="ACHA6Y_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8987344.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACHA6Y_001237"
     CDS             3424..4842
                     /locus_tag="ACHA6Y_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8987345.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACHA6Y_001238"
     CDS             4913..6382
                     /locus_tag="ACHA6Y_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8987346.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACHA6Y_001239"
     CDS             6392..6859
                     /locus_tag="ACHA6Y_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8987347.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACHA6Y_001240"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACHA6Y_001240"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8987348.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="ACHA6Y_001241"
     CDS             7848..10382
                     /locus_tag="ACHA6Y_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8987349.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="ACHA6Y_001242"
     CDS             10538..11311
                     /locus_tag="ACHA6Y_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8987350.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="ACHA6Y_001243"
     CDS             11304..12095
                     /locus_tag="ACHA6Y_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8987351.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="ACHA6Y_001244"
     CDS             12105..12596
                     /locus_tag="ACHA6Y_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8987352.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="ACHA6Y_001245"
     CDS             12614..13024
                     /locus_tag="ACHA6Y_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8987353.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="ACHA6Y_001246"
     CDS             13028..14428
                     /locus_tag="ACHA6Y_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFM8987354.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="ACHA6Y_001247"
     CDS             14451..14825
                     /locus_tag="ACHA6Y_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8987355.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5287 a         2134 c         3045 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttaaatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactccta
//
LOCUS       DAYYHX010000054        14769 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000054 DAYYHX010000000
VERSION     DAYYHX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.054"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(433..999)
                     /locus_tag="ACHA6Y_001248"
     CDS             complement(433..999)
                     /locus_tag="ACHA6Y_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8987356.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(999..1955)
                     /locus_tag="ACHA6Y_001249"
     CDS             complement(999..1955)
                     /locus_tag="ACHA6Y_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM8987357.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1955..2611)
                     /locus_tag="ACHA6Y_001250"
     CDS             complement(1955..2611)
                     /locus_tag="ACHA6Y_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8987358.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2981..4999
                     /locus_tag="ACHA6Y_001251"
     CDS             2981..4999
                     /locus_tag="ACHA6Y_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8987359.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5069..5275
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5426..7840
                     /gene="leuS"
                     /locus_tag="ACHA6Y_001252"
     CDS             5426..7840
                     /gene="leuS"
                     /locus_tag="ACHA6Y_001252"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM8987360.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8188..9741
                     /locus_tag="ACHA6Y_001253"
     CDS             8188..9741
                     /locus_tag="ACHA6Y_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM8987361.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10122..11906)
                     /locus_tag="ACHA6Y_001254"
     CDS             complement(10122..11906)
                     /locus_tag="ACHA6Y_001254"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8987362.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11921..13642)
                     /locus_tag="ACHA6Y_001255"
     CDS             complement(11921..13642)
                     /locus_tag="ACHA6Y_001255"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8987363.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13742..14503
                     /locus_tag="ACHA6Y_001256"
     CDS             13742..14503
                     /locus_tag="ACHA6Y_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8987364.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4797 a         2723 c         2936 g         4313 t
ORIGIN      
        1 aatcacctca aaacgattat acgaacttac gttcttgttg taaagcgata tttgaggggc
       61 aaaaaagggg cataatttga ctgatattaa gttagctatt gatatatgaa tgacatacaa
      121 aaatataaaa tcttttaaaa atgcttatat atcaatgttt ttgccattct tttatatatc
      181 tgttatatgt caaaaaaatg tgttcaattt caatttgtat ttgaataaag aggttgtgaa
      241 aaagctgtcc tgcatcaagt aataagaaca gccaatcaaa aacagcttct catgtttttg
      301 atttggttgg acttattacc gcagatataa gcttttgagc accgtgaata aagaggttat
      361 gaaatcaacg ctctaacctg agtattagag aaaaaaacgc aaaacatatc atttatgttt
      421 tgcgtttttt atttatcttt ttttctcaat acaatataaa ataggcggct ggttcttttg
      481 gttgataaac cgataattca gcacattata tgtttcctgt ggcagtttgg aacaaaaaga
      541 atcaaccata tccagttctt tttctccacc ttcatgacca tagtagacga caaggatcaa
      601 tcgtccgcga ggcacaagtc gtttcaatat ttcttccata gctgtgcgtg tagtctctgg
      661 catagtgatg attgctttat cactttttgg gagataccct agattgaaaa tccctgcttt
      721 gagattttgt tgttcagcta tcacttctcc taacgtttcg tggccttgta gaaacaatga
      781 gacacgttct tctaactgac gctgttctag cctttcacga gtagcatcga ttgcttgttg
      841 ctggatatca aaactataga catgaccagt ttttccgatg tgttctgcta gaaaaaccgt
      901 atcgtggcca ttgcccatcg ttgcatctac gacgtgatcc cctggttgca agatttcttg
      961 cagtaatgta tggctgaaat gcaaagcagt ttgcaacatc agacgctcac ctttttccgt
     1021 acatcatatt ttccttggaa gctatctctg cgaagaagtt cttcatcaat tgcattcaag
     1081 acttcccatt ttttcaagct ccacattggt ccaatcaagg aatcccacgg cgcatcccct
     1141 gtcaaacgat ggatgatgat ctcttttggt atcatttcca gctggtcgca aatgacattg
     1201 acatagtcag gacggctcat taattgaagg cgaccttcat gatagtcccg caacattctc
     1261 gttttgcgca tcagatgcat caaatggagc ttgattcctt ggatatctga atctaaaatg
     1321 gtgcgtctga cattttcttt catcatttct aaactttcgc ctggtaatcc gttgatcaaa
     1381 tgcgtacaaa cacggatatt atgtttgcgt aatttagcca cgccatctaa ataagtttga
     1441 taatcatgtg ctcggttgat cagttttgaa gtttcttcaa aagttgtttg caatccaagt
     1501 tctacccata aatacatccg ctcgttcagt tcagcaaggt aatcaactac ttcatctggc
     1561 agacaatccg gtcgtgtacc gactgataat cctacgacac ctggtaaatt gactacttgc
     1621 tcaaatcgtt ctttgattat ttcaactggc gcatgggtgt ttgtaaaatt ttggaaataa
     1681 acgatgtatt gttggacatg aggccatttt tgatgcatca tgtcgatttc tttctggaat
     1741 tggattggaa gagggtcttc aggtgcaacg atcatgtctc ccgaaccaga tacactgcaa
     1801 aaagtacaac cgccatgggc aacagtccca tctcgattcg gacagtcaaa tcctccatcg
     1861 atcggcacct tgaaaatttt cccgccaaac tcatttcgta aagcgtaatt ccaagaatga
     1921 tatcttttat ttccttctga ataaggaaaa gtcattaaaa gtccttcttt ctgtaagagt
     1981 tatctatttg ttttcttttt tctctataca ctggataagt caggcacagc caagaaagac
     2041 tgagtaagta gccgccgatg atatcagtag gaaaatgaac gcccagataa atgcggctta
     2101 tcccgacact tactataata aacatcatca gcaattgaag caatgtgcgt aaggttcttt
     2161 tttgaacaaa agctggaagc agaaatagta aagaaccgta aagaatcata ctgcctgctg
     2221 catgtccact agggaaactg taacttgttt caactactaa gtgcgccaat gaaggacgct
     2281 ctcgcatgaa aaatagtttg atcaccgggt tgatgattcc agcaattaca ataaatccag
     2341 aaacgaacca aaatgtttct gccctttttt tcttatacca catccataaa gccgcacaag
     2401 caaataaaat gatgacggta agaggattac cgaatttagt cataaagagt tggtagctgt
     2461 tcagatcagg ataagaagct ctgacaatgc ctgtaaacca ctgatcgaaa ccagaaagcc
     2521 acgacagatg gaactttacg atccagccca aaatgaaaaa caggacaaga aagaagctac
     2581 cagccacctg aaatgatgtt ttatatttca tacaattcct cctttgaagc aatgacttat
     2641 tatagcattt ttggtccagc gaaaaaaacg gtaaagagat aaaaccaatg tatgattcct
     2701 ctttgaataa ccaatattct tataaaaaaa ataactgtaa tcggaaagac attttttggt
     2761 aatgcaaatg aaatgttagg gaaataaaat cagtgttttt cgaaatcctt ttaaacaaga
     2821 acgtagacgt aggaagaact tgaaaatgaa cagaagttgt tcattacggt aaattacaat
     2881 atgattacaa aaaacaacaa agctcttaaa taacttttaa taaatattaa gagaagttat
     2941 aattattaat aatagataaa ttgaagttgg aggatagaag atggaaaaca ttgcaagaaa
     3001 agaacggaga cgtttagaac agaccaaacg ttttcgaaaa gcaaaacgag gggcagcaat
     3061 cgtgggaact gcgatggtag gctgttctgt cgctgcccca ttagtacagc cggttcctgt
     3121 tcaagcggat gaaacacctg ttcagttcgg cgcaagaatc aatacagcgg catttatcac
     3181 agaaattgct acttatgctc aaccaatcgc tcaagccaac gatctatatg catcagtgat
     3241 gatcgcacaa gcagttgtag aaagcggttg gggcagtagt gcgttatctc aagcacctta
     3301 ttataatctt tttgggataa aaggcagcta tcaaggacaa accgtctaca tggatacgct
     3361 tgaattttta aataatcaat gggtaaataa aaaggagcct ttccgacaat atccttcttt
     3421 tgctgaatct ttcaatgaca atgcctatgt acttagaaat acttcatttg gcaacggtta
     3481 ttactatgcc ggtacttgga agagcaacac gaaatcctat acagatgcga cagcttgttt
     3541 gacagggcgt tatgcgactg atcctggtta cgcaggcaaa ttaaataata tcatcgcaac
     3601 ttacggattg acccaatatg atacaccagc tagcggaaac gcaggcggtg gagcgactac
     3661 aggtggcaat aatactggaa gtgcaggtag tactggcggt tcaagcaatt ctggtaatac
     3721 aaactcaggt tcagcaactt cgaccactta tactgtccaa tctggtgatt ctgtttgggg
     3781 aatctcaaat aaattcggta tcacaatgga tcaattgatc caatggaaca acatccaaaa
     3841 caatttcatt tatccaggac aaaaactgac aatcaaaggt agccaggaaa atggctcttc
     3901 gacgaataat tccggtaaca ataccaactc ttcaggaaat gctggtacat caaacggagg
     3961 acaaacaact ggtgcgaaat acactgttca atctggtgat tctgtttgga agatctcaaa
     4021 tgatcacgga attacgatgg ctcaattgat tgaatggaac aatatcaaga acaatttcgt
     4081 ttatccagga caacagctaa tcgtatcaaa tggcggttct tctaatgctg gagcaaatac
     4141 tggctcaaat actgggaata caagtggctc tagttcgtcc agctcaagtt cttctaatac
     4201 agcttcagga acgaaatata cagttaaagc cggagactct gtttggagcg tctcaaataa
     4261 atacggaatt acgatggctc agctaatcca atggaacaat atccaaaata actttatcta
     4321 cccaggacaa caactgactg tttcaaacag tggatcaaca aacagcacgt caggtaatac
     4381 aggttcgtca accaatacaa ataacagcag ttcagttaat actggcagca gttcgtctca
     4441 aggcacttat accgtgaaag caggtgaatc tgtttggagc gtggcaaata aaaacggcat
     4501 cacgatggat cagttaatcg aatggaataa tatcaaaaac aacttcattt atccaggaca
     4561 gcaattgatc gttaaaggtg gaacttcggc aaatacaaat acaggttcaa ctacttctgc
     4621 agcgaaacca aatacaccga atacgtctgc tacgacaagt acatcatcca ctggtaatac
     4681 gatgtatacc gtgaaagcgg gcgagtcagt ttggagcgta gcgaacaaac atcatatcac
     4741 gatggatcag ttaatcgaat ggaataatat caaaaataat ttcatctatc caggacagca
     4801 agtgattgtg aagaaaggtt catctcaatc aacaaaccaa caatcagtta ctggttcgaa
     4861 aacctatact gtgaaatctg gagaatctgt ttggagtgtt gctgacagtc atggaattac
     4921 aatggctcaa ctaatcgaat ggaacaatat taagaacaac tttatttatc caggtcaaac
     4981 gttgattgta gctaaataaa atagctgttc gaagaaataa cgattgaatt ccacaaaaat
     5041 atttgttact ataaggctac ttaaatatga caacacttga aaaggaagta gtaaaaagac
     5101 tgaagtttca gagagcgcat ggctggtgag aatgtgcacg gatgcttttg aactcgcctt
     5161 tgagttgccc ttgctgaata cccgagtaag caagcggcgg tgacggccgt tacaacgttc
     5221 gaggcctttt cactaaggta aaattaggtg gtaccgcgta gattacgtcc tataaaaaga
     5281 gcaatctttt tataggattt ttttatttct tttgtgaagt ttgagaaaac aaagcaaaag
     5341 gaaataaaaa taaacagatt ctgccggttt tttcgttata tttataatag aaacaagtgt
     5401 tttcaataga acaggaggac agcaaatgag ttacaatcat aaagaaattg agaaaaaatg
     5461 gcaaaaatac tgggcaaaaa cgaatacttt caacacccat gatgatcctg aaaagccaaa
     5521 attttacgca ctggatatgt ttccctatcc atccggtcaa gggctacatg tagggcatcc
     5581 tgaaggttat acggcaacgg atatcctttc acgtgtgaaa agaagtcaag gatacaacgt
     5641 gctgcatcca atggggtggg atgcattcgg tctgcctgct gaacaatatg cgctagatac
     5701 aggaaacgat ccagctgagt ttacccaaaa aaatatcgaa acattccgac gccaaatcaa
     5761 ttctttagga tttagttatg actggaatcg cgaagtgaat acaactgatc cggagtatta
     5821 taaatggaca caatggatct ttacgaagct ttatgaaaaa ggtctagctt atgaagcaga
     5881 agtagcggta aactgggtac ctgaattagg aacagttatt tcaaatgaag aagtgatcga
     5941 tgggaaaagt gaacgtggtg gctatgatgt agtcagaaaa ccaatgcgcc aatggatgct
     6001 gaaaatcact gcttatgctg atcgcttatt ggatgatcta gaattagttg attggccaga
     6061 aagcattaaa gaaatgcaaa gaaattggat cggtcgttca gtaggagcaa atattgaatt
     6121 taaagtggct ggtacggata aatcctatac cgtatttacg acacgaccag atactctatt
     6181 cggtgcaact tactcggttc ttgcacctga actagatctt gttagagaaa ttacgacccc
     6241 tgaacaaaaa gctgctgtag aagcttatat cgaagagaca gctaaaaaat cagatctaaa
     6301 gagaacggat cttgcaaaag aaaaaacagg agtatttact ggtgcttatg caatcaatcc
     6361 agttaatggc aaggaaatcc caatctggat cgccgattat gtattatctt catacggcac
     6421 aggagccatc atggctgttc cagcacatga tgaacgtgac tttgaattcg ctcaaacttt
     6481 cggactagaa attcttccag ttatcgaagg tggagatgtt gaaaaagctg cttacacaga
     6541 agatggtacg cacatcaact ctgaatttct agacggcatg aacaaacaag aagcaatcga
     6601 taaaatgaat gaatggctag aagaaaatgg cgtagggaaa aaagaagtca gctatcgctt
     6661 acgtgactgg ctattctccc gtcaacgtta ctggggtgaa ccaatcccaa tcatccattg
     6721 ggaggacgga acagtcacag ctgtcccaga agaagagcta ccattacggt tgcctaagac
     6781 aaaaaacatc aagccaagcg gtactggaga atctccatta gcaaatatcg aggagtgggt
     6841 caatgtcgtt gatcctgtaa caggtaaaaa aggccgtcgc gaaacgaata caatgccaca
     6901 atgggcagga agctcatggt attacctgcg ttatatcgac cctcataaca aaaaagaact
     6961 agcagattat gaaaagttga agcgttggct accagttgac atctacattg gtggagcaga
     7021 acatgcggta cttcatttac tatatgcacg attctggcat aaattcttat acgatatcgg
     7081 tgttgttcct acaaaagaac cattccaaaa actatacaat caaggaatga tcttaggcga
     7141 aaacaacgaa aaaatgtcaa aatctcgtgg taatgtagtc aatcctgatg atgtggtcga
     7201 aacttacggt gcagatacac tgcgcatgta cgaaatgttc atggggccat tagatgcttc
     7261 gattgcttgg agcgaaaatg gcttagaagg aagccgtaaa ttcttggatc gtgtatggcg
     7321 tttgatcgtt gatgagaaca ataaaatgcg tgaccgtatc actacattga atgatggcaa
     7381 gcttgataaa gtgtatcatc aaacagtgaa aaaagtgaca gaagactatg aaaatcttca
     7441 tttcaataca gcaatctccc aattgatggt ctttatcaat gaagcctata aagtggacgc
     7501 tctgccttat gaatatatcg aaggatttgt tcaactactc gcaccgattg ctcctcacat
     7561 gggtgaagaa ctatgggcaa tccttggaaa tgatggtggt atttcttacg caccatggcc
     7621 aacatacgat gaagcagcac ttgttgaaga cgaagtagaa gttgttttcc aagtaaacgg
     7681 taaagttcgt gcgaaaagca atgttccccg agacttagga aaagatgagc tagaaaaagc
     7741 agcattagcc aatgaaatag tgcaagaata tattgaaggc aaaacagtca gaaaagttat
     7801 tgcggtacca aacaagttag tcaatatcgt tgctaattaa ttggctcttt gtcaataagg
     7861 actgatgagt tgtgcaaaat caaatctggg ccagaatgaa cttcattctg tctcagattt
     7921 tttgttattt caaattgatt aattgattga tcaaaaaggt tttgattttc atgttctcaa
     7981 acacgttcaa tgtctttttc ttctgaatgg tcttgtaaga gatctctgta atcatcaaat
     8041 ttttgtctat taatcttagt attttttgat gtataaacta cctgaaattg agagtacata
     8101 ccaaattcat aaatttttta ttagtgggac tagtgattgt atggattttg acgcataatg
     8161 aatgaagtat gagaaaagga gagacaaatg gatcaagaac tatttattat cgaagtatta
     8221 tcagctaaac aaaaaattat cgagaaagcg aaaaaaagag cgaaaagaat aatgttcaag
     8281 cttttaagct tagtattttt ctctggtttg ctttttttcg gagctgtatc gttcctatat
     8341 cgccatatga gtgcagtagt ccgttcagct gaagggatgc aaaaattaaa taatttgtat
     8401 cacttttcgt ggggagttgg cggaataaca attgttgtgc ttattgtttt tcttctactt
     8461 atttggctcc gttaccatca attgtcgcgt catttcgatc aatggcttag tcaaagagca
     8521 caacagtttt tacataataa accaacttct caagatgatg actatcttta ttttgagaag
     8581 acttcgacga gtcaagcttt tcggatacaa aaaagtgatt gccagttatt gctgagatca
     8641 cttgatgatt tacctatcta cttaggcgaa agcaaatcaa aattcggaat gaacacattc
     8701 catatttttt tactagaaaa cgaacccaga tgggaaaaaa gccagtcttc gcctggtgga
     8761 tacctgacaa ctaagatgag tacattagtc actggttttt tcctcattct tttagctgga
     8821 acaatctatt atgggagata cactcagcca aaaatgtctt ctcatataaa tgctgcttct
     8881 cacatgacaa tgaactcaac taacgatacg catatacaaa agacaaatga agggttgtta
     8941 gctgatccta agcaaaaggt aaatttgaca acacaaaaga taaatgatct caaacttgat
     9001 acagagacgc actctctcta tatgacaaca gatagtggtg ctaattgggt atttgtgccg
     9061 attgattcgc aatggttgcg ttttggcgaa tacaccctga caacggggct gattcctgaa
     9121 ggttattgga tggacaacac atatgatatt tccgccgatt tctcatggtt tatctattct
     9181 tctggggatg atgtacgtct gctgacttca agagataatg gtaaaacatg gcaaacgtct
     9241 attatcaccc aatcagttca gccgatacgt tatcgaaaag cggtatttta tggcaacgat
     9301 ggtctattta tctgtacatc agccagtggg atgtctgcag aaactttgta catttacact
     9361 actatggatg gcggaatcac ttggataaaa agtggaagta caatgatcaa tcagccagta
     9421 caaaatgtca gcttcgttac aacttcttta ggttttgttt cgacacgtga aagtttgttt
     9481 tatacaaaca acggaggacg ctcattcaaa gaatcagttc tgtcgattcc ggaaaactat
     9541 cagacagggg ggatagatct ttttcaatca ccaaatgaag ttactcaaat aagcgcgaat
     9601 acgttagaga caaaatttta tttgttgaaa aaaggaccaa tcgatgcagg gaaaatgttt
     9661 gcctgcctct tccgttcaac tgataacggg gaaacatggc aattcgttca gcaactatcg
     9721 caagtagaac aaggagatta aagaagagag attgtgagcc tgacgttcag caccgagtca
     9781 caagaaacaa attcaaaaaa cagcttctca tatttgttga atagtgtaac tcgtgattcg
     9841 gagttgacaa gagcaacaag aagcgatttt gaagtatagc ttctcgtatt ttacaagatc
     9901 gtgtcttatt gccgataggt tgtctcttga acaccgttta ttcgtaaaaa gggattgtga
     9961 caagtctttt gtcactactt accgaacgaa actgaaggag ctatccaaaa agtatcatta
    10021 gtattattct ttgtgtttct agtggcacca ctagtacatt taaaaaagct atcgatcatg
    10081 gactaatcat acatgatcga tagcttttta gataatgttt gttacgctat ctgaaattga
    10141 ctattataaa gactggcata aagcgttggt gattggagta gttcagaatg tgttcctttt
    10201 tcgacgatat cgccattctt cattacaagg atcatgtctg cattttcaat cgttgacaaa
    10261 cggtgtgcaa tcacaaaact tgtcctatct tttgtcacag cttccattgc ttgctgaatc
    10321 aatgcttctg ttcgtgtatc tacgctagaa gttgcttcat ctaaaataac gacaggagga
    10381 ttcgctagaa tgactcgagc aattgtcaat aattgttgct gtccttgaga taacgtgccg
    10441 ttttcgctag aaatgatcgt atcatatccg tctggcagtg ttcgaataaa gtgatcacat
    10501 tgtgcgattt cagccgcttc gataatttgt tctcttgtgg catccatttt cccgtaggcg
    10561 atattatcag ctaccgttcc ttcaaacagc caggtatttt gtaaaaccat cccaaaagaa
    10621 cgacgtagtt ctccacgtga aagcttggta atatcaagac cgtcgaattc gatctttcct
    10681 ttattcagtt cataaaaacg catcagcaga ttgactaaag tcgtttttcc tgcaccagtt
    10741 ggaccaacga tcgctaccgt ttgttttggc tttactgaaa aatcgacttg attcatcaat
    10801 attttatctg atgtgtaacc aaactgtaca tttttgaaag ctatttttcc tttcggttgt
    10861 tctaaaagtc ttgcatctgg aaggtctgga atttcatctg tttcatctaa tatctcaaat
    10921 atcctatcga ttgctgccat tgcggattgg atcgagttga tcacataaga agcggtagaa
    10981 attggttcag aaatctgatt gatgtactga agataagctt gtaagaagcc gatcgtcaca
    11041 ccacctgata gaacaagcat agcaccgaat accgcactta tgatgaaggc gagttgatta
    11101 atgaaacgaa tggccggata gatagcacag ttcataaatt gagcacgttt aaaagcgttg
    11161 tattgctttt tatttacttc gtgtatagca gcttctgcat gagcttgctg attgaatgtt
    11221 ttgaccacta gattaccagc taagatttct tccattcgat tatttaaaac acccagttca
    11281 tactgattct gttcagaaat ctgtttgttt ttgttcgcaa tcttagttgt aacaattgaa
    11341 ctgccaacga tcagcaataa gacgagcaaa gtaagcttgc tttcgatata aaacagcatc
    11401 agaccagcag cagctattgt tacgacagaa gtaaaaaatt gattgatacc tgtcaataac
    11461 acttgagaaa gttgattgag accggtagtt gtccgactaa tgatgtcacc tacttgatga
    11521 ttgtcaaaaa acgacatcgg taatgttttg aatttttttg tgatttcttt tcgcaccctc
    11581 aatgtgatct tttcgcttaa cgctgccatt gtccgttcct gaataaatga agtgatgctg
    11641 cttaagactg aacagccgat cagcaataag acagggaaca acaatgcttg tttgatatca
    11701 ctgaaagaaa gctgtccaat acctgttttt cttatttctg ataagagatc atcgatcccg
    11761 attcccatga tcaagggcat ggctacgact aataggttcc cgaataaact gcatagaaac
    11821 aatccgtaaa aatatttatg ttcaggtttg atcattttcc aaaaacgtat caaagcacgc
    11881 gtgttcaacg tttttttctt ttccatcgta tcccttcttt ctatattcct tgtgattttg
    11941 caaactcctg ataagtttgg caattagaca gtagttcttc atgagtacct tgtccaacga
    12001 tttttccttc atctaaaaca atgatgttat ctgcttgttg gattgtactg aggcgttgag
    12061 caacaatcac gagagttttc tccgacatgt gtttatgaag ggctgctctt aatgctgcat
    12121 ctgttttata gtctaaagcg gagaaactat catcgaatac atagatctct gctggtttga
    12181 tcaacgctcg agcaatacat atccgctgtt tctggccccc tgaataatta gaaccgcctt
    12241 gagcaacaaa gctgtccaat ccgtcaggaa gggtgtccac tagttcttct aattgagcga
    12301 tcttgatcgc tttagacaac tcttctgttg aggcatattc atttcccatc tgcaagttgc
    12361 ttaaaattgt cccgctgaat aagaaagcct tctgagggac ataactgatg tgggagcgga
    12421 tctgttcttg agtcatttga gtaagaggaa taccaccaag ggttactttt cctatcgtag
    12481 ggtcactaag tctcaagaga agtttcgcca aactactttt tccagatccg gtcccgccaa
    12541 cgatcgctgt tgtttttcct ttaggtattg tgaaatcaat atcttctaga actggatcag
    12601 agatttcagt atatttaaat gtgacatgtt caaaagcagc tgcatttgtc tgatcaaagg
    12661 ttggttcagc tactgttttg atcgtctctt cctcaatttc gatctctgct tgcagtattt
    12721 cttctatccg tcgtaatgag gcaaacgatt tagggataat gaccaatacc atagctgcga
    12781 tcatcaagta agcaagtgtc agcatagagt attcgactac agcagtcacg acaccgattt
    12841 gtaatgctcc ttctgctaca agatttccgc caaaccacaa aatagaagca taaacgattc
    12901 ccatgagcaa aaaagcaatt ggagtgaccc aagcaaatat tcggttcact ttgatcattt
    12961 gattagcata gtcagcaaag ctcttattcg ttcgcttttc ttcatatgat tgattatcaa
    13021 aggcacggat catattaatc ccagtaaaga attctctcaa tgtgatggtg atccgatcca
    13081 tttttggctg gatactcaaa gataaaggtg cccctcgttt catcagtcga taaacaatcc
    13141 atacataaaa aacaacagag atcaatgtaa cccatgttag taatagagaa taacgaaaca
    13201 tcatgagaat ggtgaaaacg caaataaccg gtgctggtaa gatcatttgg caaaataaaa
    13261 caatcatttg ctggacatta tcgacatcat tcgtcattct tgtcaaaaga gatccagaac
    13321 taaaacgact gatatccttc attgacagct gttggaattt acggaaatat ttctcccgcg
    13381 tttggaatcc aaacctagcg gaagccaatg ctgaaaagta actagaggca actgcagcga
    13441 ttgttcctag aaacgctaca agcagcattt caactccaat tttttttata actgaggctt
    13501 cgccacttgc tatacctgtg tcgatcagct ttgctactaa ttttggtaca cctaatgtac
    13561 cggcgatttc taaacagatc atcacaaagg taaggagagc gagtcctttg tttttctgta
    13621 agaatgtttt taagatagac atattattcc tccttttttc ctgatacttt tcgattctat
    13681 accttcaagt aacttgaagg tcaatagaaa aaagaataaa ctgaaaaaga ggtgagaaaa
    13741 aatgaaaaaa atgtatacga ttggccaagt atcggaacta tttgatttgc caaaatcaac
    13801 gatccgctat tgggatgaac aaggattaat ccgttcaagt agacaagaag agaatgatta
    13861 tcgattgttt gatatcgatg atatttttat gctttatgat atcgtttttt atagaaaatt
    13921 agatataccg atcaaacaga tgaagaattt atatggaaaa acattgacgg agttatatga
    13981 gacacttgat gaaacagaaa gaagaatcca taaagaactg gtggtgatga aacaaaaaca
    14041 aaaagagata agggagcaaa aaaaacagtt gaaattgatg atcgatacaa acgaggaaga
    14101 atttcctgta gaagagatcc cttttgactg tatgatctca acggagtttg aagatattgt
    14161 ggaaatcaaa aaatttctac ctaactattc aagctttggg atgatgagta tgccagcttc
    14221 cagttcacag acgatgtatg gattttttat cgaccctagt gaagtacatt tatttactca
    14281 agacgtgatt tgggagaaaa aagatacggc tgtttatcgt cgattcttat tgaaatccga
    14341 gatgaaccat gcggaacgta ataatatttt agaaatcaga gaaagaatgt atgaaaaagg
    14401 atggaaaaca ggagaagtga tcggccagta tctattgacg aatacggatg aaaacaatat
    14461 cagaactgaa tattatcatg catggataga aatgaaaaaa taggaaatat gtgtttaaga
    14521 taactcgaaa atgatggttg ttttagttat aaagaaaaat tttctggttc gattacgggt
    14581 ttaatgtggg ggattattag tcgtggatag atagaatttt agttttttag aggaaatatg
    14641 tctttaaaaa tggaatggtt gcaattctta gcgatatata cgatgtaatt gtcctcttaa
    14701 aaaagtatgg aaaaataatt gtattgggtg ttatgtataa ttcatggtga actgagacta
    14761 atgagaaga
//
LOCUS       DAYYHX010000055        14431 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000055 DAYYHX010000000
VERSION     DAYYHX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.055"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(227..1114)
                     /locus_tag="ACHA6Y_001257"
     CDS             complement(227..1114)
                     /locus_tag="ACHA6Y_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8987365.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="ACHA6Y_001258"
     CDS             complement(1111..1980)
                     /locus_tag="ACHA6Y_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8987366.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="ACHA6Y_001259"
     CDS             complement(2117..3403)
                     /locus_tag="ACHA6Y_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8987367.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="ACHA6Y_001260"
     CDS             3676..4254
                     /locus_tag="ACHA6Y_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8987368.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="ACHA6Y_001261"
     CDS             4254..5183
                     /locus_tag="ACHA6Y_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8987369.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="ACHA6Y_001262"
     CDS             5180..6916
                     /locus_tag="ACHA6Y_001262"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8987370.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHA6Y_001263"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHA6Y_001263"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM8987371.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="ACHA6Y_001264"
     CDS             complement(7812..8321)
                     /locus_tag="ACHA6Y_001264"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM8987372.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHA6Y_001265"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHA6Y_001265"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8987373.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="ACHA6Y_001266"
     CDS             complement(9622..10713)
                     /locus_tag="ACHA6Y_001266"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM8987374.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHA6Y_001267"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHA6Y_001267"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM8987375.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHA6Y_001268"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHA6Y_001268"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM8987376.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHA6Y_001269"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHA6Y_001269"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM8987377.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4109 a         3143 c         2608 g         4571 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc a
//
LOCUS       DAYYHX010000056        14168 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000056 DAYYHX010000000
VERSION     DAYYHX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.056"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACHA6Y_001270"
     CDS             complement(<1..1835)
                     /locus_tag="ACHA6Y_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8987378.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACHA6Y_001271"
     CDS             complement(1832..2536)
                     /locus_tag="ACHA6Y_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8987379.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACHA6Y_001272"
     CDS             complement(2548..4857)
                     /locus_tag="ACHA6Y_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8987380.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACHA6Y_001273"
     CDS             complement(5052..5516)
                     /locus_tag="ACHA6Y_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987381.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACHA6Y_001274"
     CDS             complement(5516..6142)
                     /locus_tag="ACHA6Y_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8987382.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACHA6Y_001275"
     CDS             complement(6149..6514)
                     /locus_tag="ACHA6Y_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8987383.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACHA6Y_001276"
     CDS             complement(6504..6842)
                     /locus_tag="ACHA6Y_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987384.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACHA6Y_001277"
     CDS             complement(6832..7158)
                     /locus_tag="ACHA6Y_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8987385.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACHA6Y_001278"
     CDS             complement(7139..7417)
                     /locus_tag="ACHA6Y_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8987386.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACHA6Y_001279"
     CDS             complement(7419..8783)
                     /locus_tag="ACHA6Y_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8987387.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACHA6Y_001280"
     CDS             complement(8796..9365)
                     /locus_tag="ACHA6Y_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFM8987388.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACHA6Y_001281"
     CDS             complement(9337..10524)
                     /locus_tag="ACHA6Y_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8987389.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACHA6Y_001282"
     CDS             complement(10588..12315)
                     /locus_tag="ACHA6Y_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8987390.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACHA6Y_001283"
     CDS             complement(12312..12764)
                     /locus_tag="ACHA6Y_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8987391.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACHA6Y_001284"
     CDS             complement(12876..13256)
                     /locus_tag="ACHA6Y_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8987392.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACHA6Y_001285"
     CDS             complement(13253..13639)
                     /locus_tag="ACHA6Y_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFM8987393.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACHA6Y_001286"
     CDS             complement(13641..13919)
                     /locus_tag="ACHA6Y_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987394.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13982..>14168
                     /locus_tag="ACHA6Y_001287"
     CDS             13982..>14168
                     /locus_tag="ACHA6Y_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987395.1"
                     /translation="MLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGVSSYSA
                     FDNDRIFGVLVQCINDEC"
BASE COUNT         3915 a         3054 c         2232 g         4967 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgca
//
LOCUS       DAYYHX010000057        14062 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000057 DAYYHX010000000
VERSION     DAYYHX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.057"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..447)
                     /locus_tag="ACHA6Y_001288"
     CDS             complement(<1..447)
                     /locus_tag="ACHA6Y_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFM8987396.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNAN"
     gene            complement(365..1894)
                     /locus_tag="ACHA6Y_001289"
     CDS             complement(365..1894)
                     /locus_tag="ACHA6Y_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8987397.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            complement(1906..3321)
                     /locus_tag="ACHA6Y_001290"
     CDS             complement(1906..3321)
                     /locus_tag="ACHA6Y_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8987398.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            complement(3314..4147)
                     /gene="terS"
                     /locus_tag="ACHA6Y_001291"
     CDS             complement(3314..4147)
                     /gene="terS"
                     /locus_tag="ACHA6Y_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFM8987399.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            complement(4306..4536)
                     /locus_tag="ACHA6Y_001292"
     CDS             complement(4306..4536)
                     /locus_tag="ACHA6Y_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987400.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            complement(5283..5750)
                     /locus_tag="ACHA6Y_001293"
     CDS             complement(5283..5750)
                     /locus_tag="ACHA6Y_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFM8987401.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(5825..6364)
                     /locus_tag="ACHA6Y_001294"
     CDS             complement(5825..6364)
                     /locus_tag="ACHA6Y_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8987402.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            complement(6295..6588)
                     /locus_tag="ACHA6Y_001295"
     CDS             complement(6295..6588)
                     /locus_tag="ACHA6Y_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987403.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            complement(6619..6813)
                     /locus_tag="ACHA6Y_001296"
     CDS             complement(6619..6813)
                     /locus_tag="ACHA6Y_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987404.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            complement(6919..7371)
                     /locus_tag="ACHA6Y_001297"
     CDS             complement(6919..7371)
                     /locus_tag="ACHA6Y_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987405.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            complement(7397..7858)
                     /locus_tag="ACHA6Y_001298"
     CDS             complement(7397..7858)
                     /locus_tag="ACHA6Y_001298"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8987406.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            complement(8059..8910)
                     /locus_tag="ACHA6Y_001299"
     CDS             complement(8059..8910)
                     /locus_tag="ACHA6Y_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8987407.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            complement(8925..9725)
                     /locus_tag="ACHA6Y_001300"
     CDS             complement(8925..9725)
                     /locus_tag="ACHA6Y_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8987408.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            complement(9768..10658)
                     /locus_tag="ACHA6Y_001301"
     CDS             complement(9768..10658)
                     /locus_tag="ACHA6Y_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM8987409.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            complement(10660..11601)
                     /locus_tag="ACHA6Y_001302"
     CDS             complement(10660..11601)
                     /locus_tag="ACHA6Y_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFM8987410.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            complement(11834..12169)
                     /locus_tag="ACHA6Y_001303"
     CDS             complement(11834..12169)
                     /locus_tag="ACHA6Y_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987411.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            complement(12228..12356)
                     /locus_tag="ACHA6Y_001304"
     CDS             complement(12228..12356)
                     /locus_tag="ACHA6Y_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987412.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            complement(12495..12683)
                     /locus_tag="ACHA6Y_001305"
     CDS             complement(12495..12683)
                     /locus_tag="ACHA6Y_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987413.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            12761..12985
                     /locus_tag="ACHA6Y_001306"
     CDS             12761..12985
                     /locus_tag="ACHA6Y_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8987414.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            complement(12982..13137)
                     /locus_tag="ACHA6Y_001307"
     CDS             complement(12982..13137)
                     /locus_tag="ACHA6Y_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987415.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(13277..13489)
                     /locus_tag="ACHA6Y_001308"
     CDS             complement(13277..13489)
                     /locus_tag="ACHA6Y_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987416.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(13492..13704)
                     /locus_tag="ACHA6Y_001309"
     CDS             complement(13492..13704)
                     /locus_tag="ACHA6Y_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFM8987417.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
BASE COUNT         3864 a         2963 c         2170 g         5065 t
ORIGIN      
        1 attagcatta gaagaatatg ttctttgaag aatttcctct aagcgatcag aaaaatattg
       61 attcacatct gcatccatcg catattctgc aagaatttta aatacatctg atcgtatttg
      121 taataagcga ttgacttttg catagtcaaa tggtggaaaa aattcatcga taaactcttt
      181 gtattttgca tcatacattt ttaatgactt ataatttttt tccacatatt cacgatattt
      241 tttctgatta cttgtactgt aaaactccat catttctgga taagtgatat tgtgtaagtc
      301 agcttgtgat aatagttttg cttgtatctc tttcagtgct tcaggaaata cactagttaa
      361 ttttttaagt gtctgatttt cttgtttgag ccttgcttga tcctccagtt ctcgacgttt
      421 ggtccaatac ttcgtttcta tcgtcactat tgccacctcc gccaaaattg tattcactat
      481 ctggataagc acttccactt tccaattcca tcatttcatt ttcataatca acatctgtta
      541 caaatggaat ttggctctga atagttcgtt tggatacgta tggtgcaagt ttaggtaaag
      601 cttcagctaa ataacttaaa tcagttggca aagatctaga gaaagtaaaa ataatttttt
      661 caggctcaat ttctactttg tcatgaaact taagaaaagc agcaattgtt tctgcgcatt
      721 ctttcaatcc ttctctaaaa tattgctctt tagtatttgt tttcgcttct aggctaatga
      781 tttgccactt gcgagcttct ccagaactgt ttgatttaaa cacctcatcg ttaaaatcga
      841 ttgctttaca aatggtgtaa aactgttttt tcaatagatc catatgatat tcattgaaat
      901 cttttgctaa attttttgtg acatattcag cttttgcagt aggggctttt aagttgataa
      961 tccctagctg attcatcatt tctttagcag tgcctttgct cattgttgtg ccagtaacaa
     1021 gcatgtacgc aagcttaaat tgttcgattt cgttttgttg atcagacaaa gcacgatcaa
     1081 ttgcatctcc aatttcctca gctacttcaa aatcacaata acgattcgta ttatttttaa
     1141 attctgccaa ggtaatcgtc cccaaagggt tatcggtctc atcaattttt ttgaacgttc
     1201 cgcttgtaac aaaattcaaa tcaccatagc aagcatatgt cagaatttta tttcttgtca
     1261 taactttcat ttcttgaaaa aattttttct ggtaagagtc atacttttct ttgatataga
     1321 ttccggcatt tccatatctt tctgctctcc aaggttcaat attacttgct cttaacttcc
     1381 aaccatcatc ttcttccaca ggctctagca atcggaaagc tactccacaa gctccttgaa
     1441 aagttgcagt ttcagaatca agcattgcga atctcatgga atcaagattg cttgttaatt
     1501 tgttaaaagc ttctggaact gtcggaagtt ctatatcatt ttttaagaat ttatctttta
     1561 atctttgaat gagtgttctt ttttgttccg atacatcgta atcccatttg ataggtatac
     1621 cagtaaaatg attgactgct tggtctacga caattgaata cataccagca tgaagtttat
     1681 tattaacttt tacaatatca gtgttaggct ttggtctatt atctatttca ttcttttcgc
     1741 tagtataagc tagatatttt ctttcgcgat cagcaaaaaa tggtttcata tcttctataa
     1801 aaccattcgg atcaaacatt ccatcttcaa tttgtgttgc atatttaaca cgtagcttct
     1861 tatatctctc taaagataat actgtactaa tcaaaagctc acctcctaaa attgaataaa
     1921 ttgatagtga cttggtggct cataaaacgc taaagccagt gcgtcagcaa tatcgggact
     1981 accaatattt cgctttttca tatcatcttt actttctaaa cgtatacgac ttctacttgt
     2041 cattttgaat ttgcgagtac ttaattcttt gattaacgaa ctatcagaag gcaattcaat
     2101 aacaggctgt tctccgttaa gatttgctgt catattttct tctaacattt ccttgatgtt
     2161 tccccatagt tgagtaccta aattatcgta aaaatcgtct tctgatgttg aaccgttatt
     2221 cactccaaac acctcaaaag gataatgttt gtcttctata agttcttcta ggcggtcggt
     2281 tacaccacct ccgacaccag tgtcatcgac tttaatcatc actttatcaa tactcggata
     2341 ttgactcatt agattcttgg ccatgttgat gacatatcct gttgtttcca tggtgctacg
     2401 ttttgaatac ttctcatact ccaatgccct ggtagcaatt ctaggaaaga gaatcgtaga
     2461 atcatcacca tatcgagcta cgtcgacacc aatatgagca acctttgttt tattgactaa
     2521 agaatcacta atttgttttt ctgtagccaa ttcaactgtt tcaagactga taaatgaatc
     2581 caacgcacct ttcggaaatt ctccaaaaat acggacacga gcaacatcac tttcttttcc
     2641 atatttttta agaatcattt ctatattgtc tttgtttgta cgtttactat catagcttga
     2701 tactttatga actctgtatt tatcacggtc tgaattatgg gaatcataaa agacaccttc
     2761 aatattattg gggtttccac acatcaacag cttattatca aaacctgata gcgtaccaag
     2821 aatagcttcc ataattggat cagacacacc agaagcttca tctaccacaa tcaacatatg
     2881 gtcctcgtga aaaccttgca tactttctgg tttagtcgct gttctagccg tagcaaacca
     2941 acgctctgaa tcaccaacca tataaatttt ggtctttgtc cacttcagta agtttttgat
     3001 caagctatca tttaaccatt tagctacctc tgcccaaagt acatcgtaaa gttgcttcat
     3061 tgtcggagct gttgctatta ctttagcgta gggtcgacac gttaagaacc aaagtattgc
     3121 tcctgcttct aatgctgttt ttccaactcc ttgaccagaa cgaacagaaa cctttgaaaa
     3181 ttcagctaaa tcatttaaaa cattttcttg ccattcatca ggattcaaat gcaaaatatc
     3241 ttggcaaaaa gctactggtt tatcatagta ataatcaatg gcagaaccaa tatcagcaaa
     3301 aggaataaat tcattattca ttttctttca ccgcccgttt gtttgcggca tttaaaactg
     3361 cctgtttcca ttcttctatt tcttcacctg aattatttcc gccaatttca tttgtctcag
     3421 cttggatctt atcagtctga gctttaatta aatcagttct gtattcattc atataaagtt
     3481 cattcatttg cttgatggct ttagctaact ggttgctaat ccgagtcaat gaatcttcaa
     3541 ttgaaagaat atcatcaatt ttgcgatgtt tttttctgct aatctgcaca tcttgcataa
     3601 cctcacgctt gatttctagc tttttaccat ttttttcaat cggagtttta atctttctta
     3661 gctgctgcaa tcgttcgact tcttcatcat ttagtccagc ttcagcttgt tggattcttt
     3721 tcatcattcg aagttgtctt attttaagaa gtcgtatttc ttcggacaaa gcaaaagaag
     3781 gatcatcatt caaactagaa tagatgtcct tctcattatc ggttaacgtg tcaaaaaata
     3841 ttgtttcata ctcacctgtt ttcaaagcgt ttttattacc cactggcgga gaagctcggc
     3901 tatttccttt gttaccttta gcattttgat taccaatagg agcgccacct tgattagtaa
     3961 cgttactttt gccattggta acgttactaa tcaattcagc gctccatttg tcttgcgatt
     4021 tccattttct aacctgagaa tctgaaacat ttaattcaga tgcaatttcc tttaactgct
     4081 tttctccgtt ggattctaac caaattcttt tggcttcatc acgtcttgga tcacgttgtc
     4141 ttgccattca atacacacca cctcacattc tgtttaggtt gagttttgtt ttctaatttt
     4201 caatatcttt tagcattaaa tcagcttcaa tcaatatctt taaatcggaa actttgtcta
     4261 acttgatttg tcctgattgg agatttttaa gccatttctt gcattctaat ccctcaaatt
     4321 cttgttaata ttattttgaa tgtttggttc atcatagaaa ccatgaccgc aataaacgag
     4381 cttgcacgca tcaatctcac atggttctgc ttctctcccc atttcgacaa tagagtactt
     4441 ccctttgatt tgtacagaac gcacaacacg cactgaacaa tcatcaatgg ttcgaggata
     4501 ttcattagtt agcgatacat accagtagtt tctcattatg tagcctcctt tatgtaaaga
     4561 aaaagaccac tcaaagagtg atctaatatg taatgcacag gcagggacgt ttccgatcct
     4621 gtgcttgaga catttgacga ttcatttgta ccgaatccca aatctcaatc taacctaacc
     4681 agttatgtaa tagcaaccta cacgatgcac aaaacgcgta cgaaattgcg cacccctata
     4741 tttttaaacc gccgatgcct cggttgccaa agtcactggc aaggaatcga accttgcatg
     4801 gttgccgaag cattgaccta gcacacatgc ttagcgtcta ccctttccgc cacagtgaca
     4861 ctataaaatt attcttggct gctactattt tttattttgc ccatttttaa atccaatcat
     4921 atatacatta agacaaagcg caaaaattga aattattaat ggaatcattt ctcttcactt
     4981 acctttagtt atcgtgtgaa taattaaaaa aacaatagac agcaacggat gatagataat
     5041 aagaacaatt tagaaggagt tgaaattcac atccttattc ttaatatttc cgttgctgcc
     5101 tatcgaagct taattaaacg atgagggaga tttcctccct tacattttat tttgtcgatc
     5161 ctgtttccta atttttcgac actatcataa tacaacgttg aataggtaag tgattggtat
     5221 aaaaaaggta taaaatggaa accaaattgg taataaaagg gtataaaaag tgtaaaaact
     5281 ggctacttga aagcaaccag ttctaacgat gaagcaaatt ggatgataat tctgtttgat
     5341 tctactttaa ccgattcttc gctagtatta ttcctttgag cagttacata aatgggcaga
     5401 ccattgatat aacgatcata gaatatcttc ttgcgccttt cagtcacatc aggcttatgc
     5461 ggatgctgta tcgctgaata gcctcgaaca aacaatttat gcagatactc aaattcttcc
     5521 tgtgcttctt ctttatcgat cagcattctt tctgcttcaa atatatgatc agctgtagaa
     5581 ggtggaacca aggaataaga tgctgtcact tttggttctc gatgttgccc tactctacat
     5641 ctagctgata gatatgctga aagaaacaca gcgacattat gttttgtgcg ttccatatca
     5701 acatctttcg cgcttggtgt ctcatatttc tttacatcga aaagtaccat cctctgattc
     5761 ccccaattat gatataatac ttatgtcgga aatattattc atagtcggag gaatccgact
     5821 ttttttattt tgtacgtgaa atgagttctc ctgatttata cgcctcagca aattcaacca
     5881 aagctactgc cttcattctc tcgatagttc tatcggaata tcctaatttt tctcctattt
     5941 gatacattga catcttatta ggaaaacaat aactataata aagtacctgg aaatggattc
     6001 tacttagccg tgttaatgct tcgataaccg aatctcgagt acttatcgct tcaatgattt
     6061 cttcgttttg actgtcaatt gtgaatttca aatttttggt aacagtcatc acggaaaagt
     6121 ctattttcac tggtccgacc aaacgttcta aatttctgta tctttttagt aatcgtctag
     6181 catttttcct tgtttgctta atatctatat tctctaaaat gttcacaatt gcctcttctc
     6241 cttccagttg tggaataacg ctcattgttg taggatagtt gtaaaaaagc aaaattaaga
     6301 ttttataact tccatgtcca ccaatctcac cactgctaaa ttctctttgc ttttcgctaa
     6361 ccacttatca cattccatcg tgttttcaat gcgaatgatt gctgagtgat tatagacgtg
     6421 ttctacatat ccacgaaatg gatagatgaa tccttctgct tcgcagcgaa ccatgtcacc
     6481 gactttgaat tttggtttct tacgtgtttt aggattcttt gtcggcatat ctagcattaa
     6541 accgccgatg ccgtggccgc tggagtaaaa tccgtctttt agtttcattc cgcttcctcc
     6601 tctttagctg tcaattactt actcgcaagt cactaaaatg gctacttcat cgataaagtt
     6661 tccgtcttcg tcttccattc cttgctgaat gctaatgtcg gtaatctgct taaaaaattc
     6721 gttgtcatag ccttgtgcgt ttatcactac ttcgacgtct ccgaaaacct ctttgagact
     6781 attaatttta tttactactt ccgatgcgtc catgactaca cccctttcca gttaaaaatc
     6841 tctgtcgtta atagactact gttcaaagta ttcatcttca ctcatccttc tgcctcctct
     6901 ttagttgtca ttagtggact aaatactacc tgtttgaaaa ccataatgtg cagtttcaac
     6961 gccgtcataa tcacttattt cccactcagc cccatcgggt atatcaacga ctttaagact
     7021 tgcatatggt ccactggcct cacttcctag tttttcgaca acagcaacga ggtccttatc
     7081 acctctgttt gaatcacttg aaaaactgga atcatcagca attccgtaca gtcggtcaat
     7141 ttcgctataa attcctgtaa ctttgtcgat tttcgggtct tttttgaaat agtagataaa
     7201 atccatcgaa cctagctttt ggcctgtgta tctcgtgtat tcatcatctg agtcatagct
     7261 aaagttattg atgtatggga atacttcaat attttttctt ttaaaaattt ccatttttgc
     7321 ttcatggctc aacccaaagc caccaaaaca tttatttaat acaatcttca ttacttttcc
     7381 tcctttgttt cctccgttag ctgaattttt tcataaacac aagccaatgt gttttgcttc
     7441 gtttattacc aaacaatggc tgttctccaa tagcctctaa cacatcagaa agtggtatct
     7501 gttcctcgtt ccacttaaaa attaatgttc ctgaaggtcg taaaactcgc atacactctg
     7561 caaaaccttg cctgatgtct tctggccaaa gttcgtctaa ttttccatat tttttggcaa
     7621 gccatgagtt ttcgccgaca tgtcttaagt gtggcgggtc aaatacaacc atatcgaaca
     7681 catcatcttc aaaaggcata tcgcgaaagt ctgcgaccac atctggatca acattgatca
     7741 catgacctgt tgataattct tcatactgct gacgacaatc cataaatgtt acgtcagaat
     7801 tgttcttatc aaaccagaac aatctactac cgcaacatgc atctaatatt tttggcaatt
     7861 actctacctc caataactct ggattctcgt agacgttacc taaaacagtg agaaatcttt
     7921 ctgtatcccg ataaataatt agcttactta tcttccgaac cattcgactc tcttgttgag
     7981 ataattcgtc catgtcacga tcacctataa tttgcaacat tatctgacga cagattcttt
     8041 tttcttgttt agtcaaattc atgcgatcgc tcctttgact ggttttatgc gcttgtctgc
     8101 tgtttgagaa aataccatcg taaatccatc agagttgaca aacattcttg atattgttct
     8161 cgtaccgtaa gcttttttca gttcttcacc aagcaagttc gttgtgataa tcgttgctag
     8221 gttctgtcgt gcatccaaga acgaatttag cgtgtttatg ccaaacgctc gactatctga
     8281 tgcatctttt cctagttctg aaccgatatc atcaatgatg actaaatccg ctgttttgat
     8341 atctgcaatc aacgatccct caatcgtttt tctgagttca ggattgttgt acgaaaattt
     8401 gatttgatcc agcatttcct gtaacccgat aaataagatt tttttatcgt agtttgatcg
     8461 cttcaagact tcccaagccg ctgccatcgc taggtgactt ttacctgttc cttgttttcc
     8521 agttaaaaca aggtgacttg gatttcctaa caagactgaa ttaacaaagc gtttggttac
     8581 ttcaaccgct tgtcttgttt cttgatcgac aatttgataa ttctgcaatg tgcaatcaaa
     8641 caacgcttga ttaggcacaa cagaaccacc tttgaaaaaa ttgattgctc ttgctttcag
     8701 gctctcgttg taaattcgtt ctgtttgtaa gtcttcctga actcgcaatg ctttataccc
     8761 acattgcata caggttggct tacaacgttc tgagccatca gggttcttcg tacgccatcc
     8821 atacaaaggc tgtccgcatt caggacattc gccacgttgg acaagcactt tctgtatcag
     8881 cttttccatg atttccccaa cagtttccat gcctacgcct cctctcaaat aggcaactca
     8941 tctgtgctaa atttctcgta ttcaagcgtt ttagtttgtt ttgttttata atcacgattt
     9001 gcttgcgctg ccattgtgtc gtatttttct cgcaattttt tagcagataa aatattcgat
     9061 gcccaaaaca cattgtgctg actccattca atcattcccc gtacctggtt ttcggtccgc
     9121 ttgtcgatct cgatcatttt ccgaatgtca tctgcccaac tttgcagatt cggctttttg
     9181 atttcctgat tctgacaaat ctgtttgaat aactcctccg cgagaatgta gtaaactgag
     9241 tcggtgtcgt aaacacgctt tttgcgtggt tgcgacgatg atgttttatt attcttttca
     9301 ttcttataat tcttttcatt cttgtttgtg tgcacttgtt gttcacttgt tgtgcgtttg
     9361 atgttcattt gttgttcact gctttggtac aaagaccagt tttttattga tataacgctg
     9421 tatttcgtag ttgatttgat gttcaacatt ccgttttttt caaattgttt tagccatctc
     9481 catacagaac cgctgttcac ttgatgttca cgtttcacgc cttcgttcat ctcagatgtg
     9541 atagcatcgc gccctgtgac gaattctccg ctgttcactt gtatctcttt tccattaaaa
     9601 agtatcttgc gattctcatg actcgctttc atcaaacaca agttccataa tttatacata
     9661 taaggattgg tccaaacgaa tgaattcatc acttttcgat gtaatttgac atatcctgtg
     9721 ttcattcgta tttctcctct agaaaagcaa gggagaaagc tccctcatta tttgtttaac
     9781 ggcggattag atgcatcaaa taatccagtt tgtacatctt cattttcttc agatataacc
     9841 tctgcttctt ttctttcagg aatatcttct tcaacttctg tttcagcaat aatgctgccg
     9901 tcttcttgaa ccctttggac tctctcatcc gatgtggtgg cttcttgcat ttcgatggac
     9961 aagattcccc atttagaaag aagatttctc aaaacagttt ttcgtgccat tgcattgtaa
    10021 tcagatgccc acacaccact taactttgtc ttatcgcgat ctttattgtt agcaatccga
    10081 tgagcttcga tttcttgttt ggtccaatag acagtttttt tgaatccatt cagtaactcg
    10141 aaatagccaa catatccaat gacttcatca gacgttctac catttggatc aaactcaaac
    10201 tcttctgtca gtcggttcca gctttttagt tctccttcgt aaacttcgat cacatttaat
    10261 gctttgtatt tacctgatcg ttgggctaat tggatatatc ctttatagcc aagcatgaat
    10321 tgagctttct tttcccattt tcctgtttgc ttgtttttac tattgaatgg aactaaatat
    10381 gcataaccta aattcttatc tagcccaaga tttaatgttg cagcagttaa cgcaccactc
    10441 atgatagaca tcggttcact atctgcaaga taactgtcat tagatacaag agtcataaca
    10501 ttcgacataa aagcattagc attgtcatga agcacctctt cgaacttctt tctcattgtt
    10561 ggtgtattca tcaaagcttt aagtcctaat tgacctggtg caacttgttt ctgtggcttt
    10621 gctgccaatt gattttttaa cgattcattt gttgccatat tatttgatct ccctttcggt
    10681 tagccttctt gattcagtaa cgttataaat ctcttcatca tttgcgacat ctggatattt
    10741 ctctgctagt ttcttcgagt tcatacgtct cgtacggaca aatttccaac tgatgatgtt
    10801 tttttgagtg ataccgatac tggcttcacg tttacctagc tcgctgataa tctcgttgtc
    10861 tacttgacgg atagctgatt caatttcttt cttagtccgt ttgagttcgt ttttctgttc
    10921 aactagttca tcgaaatgaa ttggtaacgt cgtttgaacg tcttcaacat ctgcatactt
    10981 ctctttcaag aaatcagctg tcgcttgact accatcaata attggctcga tacctccaag
    11041 aacgtttgtt tcccaaaact ctactaattg ttcagtgatt gtatcgatca gctcttgatc
    11101 tcgttcaatc cgcttccaga taaatttttg accaccgatt aagacagcga tgtaacaata
    11161 gtctttgttt agaacgttca tgtaatgttg aacttgacag agatagctaa gcggtacctc
    11221 ttcgccttcc cattctttac cgagaaattg attggctgtt ttgcattcca gaatggcatt
    11281 ttctcccact acttcccgat caatatttgc tcttaaaaat ggatgtaatg gatgttcaaa
    11341 tacttggttt cttctgcgta cttttttgcc tgtacgttcc tgaaactctt tagcaaccac
    11401 ttcttctaaa acattacccc aataagcggg ctcacttcct gattcttcaa gtactacttg
    11461 tcctgttttt tctagccata gttgataagg agatttccat ttattcaatc ctaaaattgt
    11521 tccaacatcc gaacctccga tgcctttctt acggtcttca agccattctt gatggctcat
    11581 ttctaaggta gatttactca tcttcatcct cctcatcaat tggtgcttca tatggtggtt
    11641 tagcataatc aggatcagtt aaatagttgt caagatttgc taactcgttc atgtttaacc
    11701 tcttttctgc ttgctaacac gcaaagcaca agcatggtaa ttgcgaataa caagccaaag
    11761 attacataac ttttcgctac aatcactaac gtgaaaaata aaataatgag tagatcaatt
    11821 gttttttcat tcatcattga aattccccct tgagaatacg agtcatcaca tcagctaaat
    11881 cttccttgtt attaataaca aaagtatgat ttgaaggttg attttcttct tcatttttcg
    11941 cttgttcgta agcatccaca gcaattctac gcataatatg tgctgacaat gggagatttt
    12001 catctaaatg ttcttcttgc atagcaagac aaagtaaaat ttctgttgtc tcaccataaa
    12061 cgagactttt aagttcccct tctttttggg ctgtacaaat aatagaaaca ccttgctttt
    12121 ggcattcacg tttcaattct tcgataagat tttcaatttt cttattcatg tgatataatt
    12181 ctcctagttt tataaatttg tatgtgactc tttgctggct ggcagagtca cttttttatt
    12241 tgttgccatg ctttttgctt ttcgatatgt tgcttgctta aaataatagg gcggctattt
    12301 ccccaccgat tatcagcaat cactttaccg atttttagcg cttcttctcg tgccatagtt
    12361 gctcctttct tttgaatcaa gcagattgat taaaaccatc aatgctgcga acaagcttcc
    12421 cccgataata ctttggtgcg ctacaatcac taataacccc aaaatgaatc ctataaaaag
    12481 tgtgtctgtc ttcttcataa tctaatctcc ctatttttta tttctagcat tctcaaatcc
    12541 tcaagttcag aagcaattag ttcagcttgc ctatctgata gctcatcggc ttttctaagc
    12601 gctgcacggt catcttgtaa ttgtttcctg cgttgtttaa tcaaaccgag aatttgatgt
    12661 tcttgttgca aggtatatgc catatcactt ctcctttcgt ttattgaatt tctccatttc
    12721 tatttgataa aatatttcta tagaaatgga ggtgaaaact atgggaaaaa atcaacatgt
    12781 tgtacctaat tccaatggtg gttggaatgt caaaggtgct ggaaattcta aagcgactgc
    12841 acatactaat acaaaatcag aagcagtaaa aatcgctaga gagatttcta aaaatcaagg
    12901 atctgagcta tttattcatg gcagagatgg aaaaattcaa agccgtgata gtcacggaaa
    12961 cgatccacac ccaccaaaag gttaatcata tttaggagtt aatcgaatac gataaccgtc
    13021 agcaggagtt gcatcggtca atgtaacttc tgctactttt ttcccttctt ctgtttcaat
    13081 aattaatctt gtgtaattgt tctcattcaa aatccctatt tttgattctt ccttcatgtc
    13141 atccctcctt cgtctctcgc cgtcaccacc tccaagaaga ggagttgcca aaatgaaaaa
    13201 acgtggactt tggcaaactt gaaaggagag cagcaactcc tcttttcaga agtggtgaat
    13261 ttagattttt tagctattaa caaacagctc taactctaaa tcgattttta gaacgccatg
    13321 ttctaaactg ttcatataaa tcgacatcaa ttgttatttc tttggaagta actgcgttat
    13381 acccctcact aaaacgtgat tttttaaatt ccttcattct tctttgataa gtactttcag
    13441 aatacttata tttctcttta aactccgctt tgctcaaacg ttgtaccatt tttacgcctc
    13501 ctgttctgat ggctgatcat tagaatctac ttctaacttg atatccaaaa gttcttcgat
    13561 ttgttttaaa cgttccttag gttttctttt ttcgatgaag atatctcgta aataagtaat
    13621 tgatataccc attcgtcgag ctaactctct ttgactccaa cctttcttta ctaaagcaat
    13681 taatacttgc tgttttaaag tcatgcttca taccccctta tcgaatattt tctaatattt
    13741 ttgtctaata agtttgacaa aaactgcaaa tatgctatta tgtaagcata caaaatacag
    13801 cgcacaaaaa gcgagcttac tgatgtctgg ggagactatt agtttattgc ttttagtctt
    13861 tgtatgccgt aaatatcttg cttacaaaac tagtataact gcaaattctc tatttgtaaa
    13921 tagataattt gcagaaaaat actttttttg tcggtatttt gaaaggaatg catatcctat
    13981 gctattagac cgtataaaaa tcttattaaa agaacgttca atgacgttgg cagaacttga
    14041 aagaaagtta aatttcggaa at
//
LOCUS       DAYYHX010000058        13866 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000058 DAYYHX010000000
VERSION     DAYYHX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.058"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACHA6Y_001310"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACHA6Y_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8987418.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="ACHA6Y_001311"
     CDS             complement(481..675)
                     /locus_tag="ACHA6Y_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987419.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            758..1366
                     /locus_tag="ACHA6Y_001312"
                     /pseudo
     CDS             758..1366
                     /locus_tag="ACHA6Y_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1386..2414
                     /locus_tag="ACHA6Y_001313"
     CDS             1386..2414
                     /locus_tag="ACHA6Y_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8987420.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            2411..3169
                     /locus_tag="ACHA6Y_001314"
     CDS             2411..3169
                     /locus_tag="ACHA6Y_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8987421.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(3255..3995)
                     /locus_tag="ACHA6Y_001315"
     CDS             complement(3255..3995)
                     /locus_tag="ACHA6Y_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8987422.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            4056..5309
                     /locus_tag="ACHA6Y_001316"
     CDS             4056..5309
                     /locus_tag="ACHA6Y_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8987423.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            5604..5858
                     /locus_tag="ACHA6Y_001317"
                     /pseudo
     CDS             5604..5858
                     /locus_tag="ACHA6Y_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5980..6306
                     /locus_tag="ACHA6Y_001318"
     CDS             5980..6306
                     /locus_tag="ACHA6Y_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8987424.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            6374..7087
                     /locus_tag="ACHA6Y_001319"
     CDS             6374..7087
                     /locus_tag="ACHA6Y_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8987425.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            7397..8249
                     /gene="licT"
                     /locus_tag="ACHA6Y_001320"
                     /pseudo
     CDS             7397..8249
                     /gene="licT"
                     /locus_tag="ACHA6Y_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            8370..10235
                     /locus_tag="ACHA6Y_001321"
     CDS             8370..10235
                     /locus_tag="ACHA6Y_001321"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8987426.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            10249..11692
                     /locus_tag="ACHA6Y_001322"
                     /pseudo
     CDS             10249..11692
                     /locus_tag="ACHA6Y_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11800..12501
                     /locus_tag="ACHA6Y_001323"
     CDS             11800..12501
                     /locus_tag="ACHA6Y_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8987427.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            12627..13139
                     /locus_tag="ACHA6Y_001324"
                     /pseudo
     CDS             12627..13139
                     /locus_tag="ACHA6Y_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(13537..13746)
                     /locus_tag="ACHA6Y_001325"
     CDS             complement(13537..13746)
                     /locus_tag="ACHA6Y_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM8987428.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         4633 a         2354 c         2887 g         3992 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag taaggtactg ttctatgggg cgcttaccca gaaaatcaac tttttgcagg
      781 agatgtatta gaaattgtat taaacgacca agcaggagaa gctgctgcta agggtgtgca
      841 gaacaagcca agtaccaacg atgaaattgg gaatcacaat ccaagtgata cgccagttgt
      901 ttatcatgat gccccgcctt ttgcacctgc tacgaagatt gtagtagaag gtggtctctc
      961 atttcttgcc ccttccaaac tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga
     1021 agcatttggc caaatggata tacaggaaaa attatggatt tatgatcagc gagaaacaaa
     1081 aagtccatgg atcttaacga taaaattatc tcagcctttc acaaccgatg caggattttc
     1141 tatgcaggat gtcctatatt tcaagaaagc tgaggagtat caattagtga atgaagaaga
     1201 actggaaatt gtcagaggac aaaattcaac gaatgtgcaa acagattatt cagaatattt
     1261 aaataatggt cgggcgttta aacttgtctt aaccaaagaa aatcagatag ctggtaatta
     1321 ctcagcagag atgacatgga cattagcaga tgcgccgaca gaataaagat caaggagaat
     1381 gattgatgaa tcgaatatgg aataaaagtc atcgaaacaa ctgctcttta cttctgatcc
     1441 ttctccttgg attcatcctt tggcagacac ctgtccatgc ctccgaattc aattttgcgg
     1501 tagattttga attaccggct aatcagattg ataagaacaa aacatatata aatgtaagga
     1561 tggagcctgg gaaaacacag gaaatcccct acacggtctt taaccgaaca gataaatcag
     1621 taacagttga gacattgatc cactccgcta cgacgaattc aaacggtgtg atcgaatatg
     1681 gagaatcaaa aagtaaattt gatgaaacat taagttatga tatgaaaaac ttagcaaaaa
     1741 cagtgggaga aataactgtc cctcctcaaa gtgcggtcac tgagacactt tcactgaaag
     1801 ctccagaaaa agagctggct ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag
     1861 agaacgtttc taacaaaagt acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa
     1921 tcttgattcg aaatagcgaa aaagagtctt ccccaaattt gaaattaaaa aaaacttatg
     1981 cggatcaaag cgatggaaga aatgtgatta aggcgaatct tcaaaatcca aatgcttctt
     2041 atatgttcgg cttgactaca tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc
     2101 tttcaataaa aaaagaaaaa atagatgtag cacccaatac aaattttgat ttacttgtcc
     2161 cattagaagg aaaaaaatta gctccgggac attatcaagc ggttgtgaag ggtgcttgga
     2221 aagaaaaacg ctggaatctc cataccgatt ttgaaataac tgatgatgca gcggaccaat
     2281 tgaatgaaaa ggatattgat ttaaaaaatc agccagaaac atttgactgg cgatggctga
     2341 tacttgttgg agtcattctt attggcttat ttgctggatt atttatttta aaagaaagaa
     2401 ggaaaaagaa atgaaaaaaa caattgcatt aagtaccgtt ctagctggaa gtctattatt
     2461 atgtacacaa agtgtttttg ctgagaataa gagctcagcc actacagaag gaacgatttc
     2521 tgtaacaaaa ccagatgccg caaatcctga agacgttgtt ccagttcctc ctgttgatcc
     2581 aaatgatccc gatacgccgt ttacacctaa cgaaccaaat cagggaacat ccggattact
     2641 gacgattgac caagctccag atcttgattt tggaacaatc aagttaggta cagcaaaaac
     2701 agtatatgct gcattacaaa acggaacgga cagtacaggg acagcaacac aagtacagaa
     2761 tttcgtacaa gtaacggata aaagcggaat ttatgccggt tggaaactat cagtcaaacg
     2821 aacagaattt attggtacaa catctgatac gaataaacta actggttcaa aggtgaaatt
     2881 caaaaacgcc gtgattcgaa atagcgcaac taatagcagt ggaacgccat ctactatttc
     2941 tggtactggc gctgaaggaa ttgagatccc agcagatcag gcagtagatc tagtaactgc
     3001 aggagcaaat gaagggattg gcacatggat ttattcatta ggagagaatg tggaacaagg
     3061 gagacaatca gtggaattat ttgttcctgt atccaattat gccgtagaca actacgtttc
     3121 aacattcaca tggtcgttaa cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa
     3181 tgacttcagg gagtggagcc gattcaaggc caactccctg aaatctgcta tgcttacgtc
     3241 tcagctctta ttcatcaact cgaagcagtt ttttggaagt catggaaacg ttcctttgtc
     3301 ttttcaaaga tctttcgacc aacgagtaag gagtcaccat tgaaaaattc taacacataa
     3361 tgagcagaat caattttctg caattgcgat aagttgatac agtaagattt taatagtaaa
     3421 aagtaagcat acttttccaa ctctgtcttc aattgtttta aagaagtatt cactaattta
     3481 tcttctgtag ttgttttgac cataacttgg tttttcaaac ctttgatact ttcaatatat
     3541 aaaatttccc gaggatacag gacgagactt ccaccttttt gatcaataaa gattttatcg
     3601 acatttacga tttgctgttc tttccaattg atctgttcta gcacttgacg aatttgttca
     3661 gataaattcc tgtcttttct gatatatcca atcggaagga ttttccgatt gatgatctct
     3721 actgcaaaat tcgtataagc ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt
     3781 atttgttcgg cgacatcaat acctgtgagt gattgcttca agtcgatatc gataaagaaa
     3841 acatcgtgat tatcaatagt acccaactgg atcaactgat tgatttgaga gatagaagtg
     3901 agctgcacga tattctcgat atgatgattg accaattctt tgatgatcca ttcgctgtga
     3961 aactggttat cttctatgat aaaatacttt ttcatatgca ggctcctatt aaaaaaatat
     4021 gtgtacattt tgaaacaaaa caatggagga ataagatggt cgcaacagtg acactttatt
     4081 tgacctttac tttgatttta gatttaggta tctatttatt cttttcgaaa caactgctag
     4141 taaaaaacag ctttggtttt atagctttga tggtggtgac acttttattt cctatcccta
     4201 aagtgagctt tccttaccta gttggcttat tattgatgtt tcttttttat tttactacga
     4261 aaaacctgtt tatcagctgt ttgatcagta atcttttttt tgctatcact ggactcagct
     4321 tttttctggt ttttgatctg atggattatt taggaaagag acatcttgga acagcaagtt
     4381 tttttgcact acttatcgca tttattttct atctattagt aaaaagtata gacaaacaga
     4441 cgagatttct caacttgctt tttacttatc caactgaaca ctattttgga ggtatttgta
     4501 ttttattaag ctggggcgtg atttacttgg tattgggaag tgttcacttc cgctcatcta
     4561 attatctgct tctatctctt ttgtcgatag tcatcagttt tttttctttg tttttttcca
     4621 ttatgttgat cagtttccaa gtaaaagaaa gacaacatca ggaatcgctt caactgtatc
     4681 aaataaatga agaatattac catcatctag aggaattcaa acatgattat aaaagtctac
     4741 tgttttcatt aaaaattact cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa
     4801 tcttagaaga tcattctgca gctatttttg aaaaaccgca aatacaacag ctaacaaata
     4861 ttgtatcccc agctgtcagg ggtgtctttt taaaatttct agaaaaagca gaatcggaga
     4921 aaatcccgat ccaagtagtt attcctgtcg aagtgaaaac aatcccgatt gatctggttg
     4981 tttttattcg ctgtttatct atttttattt ctaacgcatt cgatcatcgt gtagccgatc
     5041 agagtccgat tacgatcaat ctgctggaag aaaaagaggg attacgtttt agtatagcga
     5101 acaaggcaaa tccgacgaat ttgacgctat cggagatggt caaaagagga aaaacctcta
     5161 aaaaaaatgg cgggttaggt ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg
     5221 cagaactaaa aatcgaattt tcaaaacaga atcactattt ttttgtagaa atgtatctgg
     5281 catctagaaa gagcaaatca gcctactgac ttactgtcaa aggataagct tgaggaaagt
     5341 ctacacggag atagagacaa caacacagca tattcaaaag aaacagaagc gtgatgaccg
     5401 ttgtcaccct tctgtttctt ttgctatgta gttaaacaaa gtagttgaca cttccgctac
     5461 ttagtattag tacgaaagta tacttcatga tactaagtag ataagaagtg aaaagcggaa
     5521 cccacgatcg tcaaaatgct aactgctttg cttacaccag ataaagggaa gagcaatatc
     5581 gcttatttta ggcgtggaga agcaagaaaa tccctacgat cacacaaaaa atgctgaccc
     5641 agcagttaag agagttagaa caagatggca ttgtccatcg aaaagtctat aaccaagtcc
     5701 cgcctaaagt agagtactcg ctcaccgaag aaggaaaatc attaagagag attctagtag
     5761 cgatgtctgt ttggggagaa gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca
     5821 cattactgca tacacatggt ggctacctaa attactgatt tctatgacat ttattttaaa
     5881 taaaagaaaa caggagtttt tttaaaagca tcatctattt tttatgtaaa aaaatggtat
     5941 gattaatcaa gtaagaaatg attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt
     6001 aaccagttta gagatgaacg taattggcga caatttcata acgaaaaaga tttggctatt
     6061 tctatttctt tggaagcatc tgaattgtta gaactttttc aatggaaaac gccggaagaa
     6121 gtaaaagaaa cacaattaga acgaatcaaa gaagaactgg cggatgtatt gatctattca
     6181 tacatgatgg cagacaacct tcagctggat ctagatgaga tcattgaaga aaaattgatc
     6241 aaaaataatt tgaaatatcc tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag
     6301 ttttagaaat gtgtcattcg gctgttggaa agagcaaaag aaaaacagta aaatgagtct
     6361 ggagggagag aaaatgaaaa aaaaaggaaa agcaaaaaga tggctagtca atatcctctt
     6421 gttcctcttg ttattagtag gactagcttt gattttcaat gaacagatca aggattatct
     6481 tgtcagagag acaggagata aatatgctat agcgaatgta acaaaagaag atctgaaaaa
     6541 gaataacgat aaagatgtca gttttgactt tgatgcggta gaaccaatga cgacagaagg
     6601 ggtcatgagg tcgcagatga gaggaacaga tctaccagtg attgctagta tcgcagtccc
     6661 ttcagtttca atcaatttac cgattttcaa aggcttggat aatacgtcac tactttacgg
     6721 agcaggaaca ttatctccag atcaagaaat gggaaaagga aattatgcct tagcaagcca
     6781 tcgtgcaacg aaccctgaat tgttgtttac accgctggaa aatttagaga tgggtgccaa
     6841 gatctatttg acagatttag aaaatgtgta tacatacaaa acatttttta aagaaaaagt
     6901 tgctcctaca gatacccaat tactgaatga agtcgaggaa aaagaaattg tcacactaat
     6961 tacttgtggc gacatggacg ccgtcacgcg actagttgta caaggagaac tggaaagtgt
     7021 tacaccgata aaagatgcga cagatgatat gagatctgct ttcaatctag agacaaaaac
     7081 attttaggat actgggacat aagtcgttac agattaaaaa cagccaaagg atatgaatcg
     7141 ataaggaaaa accaatttgc tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg
     7201 gttgttttct agatagactt gtgttccagt ttttttattt tttatatgaa aaggcttgca
     7261 taaataaaaa ttcgatggta tgatgatttt gaataagaaa tggattgtta ctgtacggca
     7321 ggcaaaacca gaattcagct aagaaacagt aggcgaattc taggtttttt tatttttctt
     7381 gagagggcga agcaaaatga aaattgataa aattttgaat aacaatgtcg tcatttcgaa
     7441 aaatggattc ggggaagaag tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga
     7501 ttggggacga gatttcgcca gaagcggtcc aaaaagaatt tattttaaga gattcttttg
     7561 ctaaaaacca attccagcag ctgatggctg atattccggc agaggagatc gaatgggtca
     7621 aacaagtagt cgaactggct gaagataaac taaaagttga attttcgcct aatatttatc
     7681 tgacattgac cgatcatctt cattatgcga tcacacgagc aaaagaaaat atccaactgc
     7741 ctaatccatt attatttgaa acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag
     7801 aggctctttt gttcgtgaaa caaaagacgg gtgtggaact ttctgaggat gaagccggtt
     7861 ttattgcctt tcattttgtg aatagccaac aaggaaacga agacatgcaa gtgacgatga
     7921 ccgctaccac aatggtaaga gatatactga gtatcatcag taaattcttc ggcatcgtgt
     7981 tcgatgaaga atctttgaat tatcagcgta tcattactca tctgcagttt tttgcccagc
     8041 gttatctcaa aggggaagga tctgacgagc aagatgaatt tctctacgca ttagttcaag
     8101 gaaagtatcc aaaagcgttt cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc
     8161 aaaaacagga aatggggatc gcagagcaga tttatttaac gattcatatt caaagagtag
     8221 taagtgagaa aaaaagcatc caatcataag gattgttact gtatggcagg caaaacctgg
     8281 attgaaaaaa taggttcacg aatgtttgtg actgtttttt tcaatctggg tttttatttt
     8341 ttaaataaaa aagaaagaag gaaaaaacaa tggataatca agcagttggt cgtcgtgtat
     8401 gggaagcggt aggaggacaa aagaatgtca aaagtttagt acattgtgct acgcggttgc
     8461 gattcagatt aaaagatgaa tctttagcag atacgcaaaa gttaaaagaa gacccggacg
     8521 tgatccaagt cgtacaaagt ggcggtcagt atcaagtagt gattggcagt aatgtagcag
     8581 atgtttatca atcgatcgtg gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag
     8641 agtccaaaaa tattttgaat cgtctgatcg atattatctc tagtatattt acaccttttc
     8701 taggggcgat ggcagccgcc ggggtattga aaggattctt atcattggca acggtgatgg
     8761 gctggctgac agctgatagc ggtacttatc aaatattgtt tgctgcagca gatggtgtct
     8821 ttaccttctt acctgtcatg cttgctttta cagcagctaa aaagttcaaa gccaaccagt
     8881 ttttagcagt agcaattgct atggcacttg tgtatccagc tatcacagca gttgctggtg
     8941 cgggagaagc tatcagtttc tttgggatcc cagtgattct ttcaccatct ggttatactt
     9001 cttctgtcat accaatcatt ctagccgtat gggttcaaag taaattagag ccattcgtaa
     9061 aaaaagtgat tccgcaattt ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga
     9121 tggttccatt aacatttctt gctttaggac caatcgggac ggttgccggt aatgctttag
     9181 gcggtctctt caactcgatt tatggattta gtccaatcgt agcaggtcta atcatgggaa
     9241 gtttatggca agtattcgtg atgttcggca tgcattgggg attcgtaccg atcatgtttt
     9301 tgaatatcga acaatatggg tttgatgtat tgatgccaat gcttcttcca gccatcttgg
     9361 cacaaggtgg ggctgcttta gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat
     9421 tagggatttc ttctacagtg acttcgttat ttggaatcac tgaaccaact gtgtatggtg
     9481 tgacattacc tttgaagaaa ccattcattg ccgcttgtat ttctggaggt attggtggag
     9541 ctattatcgg attttccgga gtaaaagctt tctccagcag tttggtcagc ttattgacga
     9601 ttccgacttt cattaatacg gtggatggtg tagagtcaaa tgtgacagta gcagtaatag
     9661 ctacaggaat tgcttttgtt cttgcattcg taggtacatt gattttagga tttgacgaac
     9721 aaacacaaga caatcaatta gaaaacaaac atgcgaatgc aggagaaccg attacttcag
     9781 cacggcatac cttaaaaagt cctttaacgg gtaaagtact gccattgagt gaagtaccag
     9841 accaagtttt ttcttctggc gtgatgggaa aaggtatcgc tattgatcca gaagtaggag
     9901 aactagttgc acctgcagac ggagagatca cgactatttt tccaactgga catgctgtag
     9961 ggatcacaac gactgacgga gcggagattt tgattcatat cgggatggat acagtagaat
    10021 taaatgggaa tggctttgag atcttagtca aacaagggga tctcgtcaaa gcaggagatt
    10081 tattgattcg ttttgacatc gaagcaatta gagcagctgg ctatagcgta atcactccag
    10141 ttgtcattac gaatactgat gcatttgccg atatacttga attagaccaa aaagagatta
    10201 ttgcaaacga agatgttctg gcaattgtaa aataaggagg aaatcaggat gcaatcacgt
    10261 ttttcagaaa aatttttatg gggtggagca actgctgcca aatcaattag aaggggctta
    10321 tgatcgagac ggaaaaggac tatccgttgc agatgctatg cctggaggta agcagcgctt
    10381 tgcgattatc ggcagtgaag aatttgactg gacgattgat caagaaaaat atatttaccc
    10441 gaatcatcga ggaatcgatc attacgatcg tttcaaagaa gatcttgcct tgtttgcgga
    10501 aatgggattc aaatgttatc gtttttcgat tgcctggaca cgaatttttc caaatggaga
    10561 cgaaggtact cctaacgaag cgggattaga attttatgat cagctgattg atgagtgctt
    10621 aaaatatgat attgaacctg tgatcacgat ttctcattac gaaatgcctc ttcatttggc
    10681 aaaagaatac ggtggctgga aaaatcgaaa actgatcgac ttctatgaac gatttgctca
    10741 gacagtatta gagagataca gcagtaaagt caaatattgg atgactttca atgaaatcaa
    10801 ttcggccttt cactttccag ctttaagtca aggattagta aaaagcaatg gagcaggaga
    10861 gtaccaaaat attttccaag catggcataa tcaattcgtt gcaagtagca aagccgtaaa
    10921 aatcggccac gaattgcgtt cggatatcca aatcggctgc atgattatct atgcaacaac
    10981 ttacagcatc gatgccaatc cagtcaatca ggcagcaaca atgatccaaa accaagaatt
    11041 caattttttc tgcacggatg tccaagtacg tggagaatac cctgcataca cagcgcgtac
    11101 ccataaaaaa tatggagtcg atccggaaaa attagagcaa acggaaaaag acttccagtt
    11161 gctaaaagaa tatccagtgg actacatcgg atttagttat tatatgtcta cggcaatcaa
    11221 tgagactgat ccacaagcag caacttcaga agggaacttg ctgggaggag taaaaaatcc
    11281 gtttcttgag gcaagtgaat ggggctggca gatcgatcca gaagggctga gaatcgcatt
    11341 gaatgaatta tacaatcgtt accagaaacc attattcatt gttgaaaacg gtcttggagc
    11401 cattgatcat atggaagagg atggcacgat tattgatgat tatcggatcg attatttacg
    11461 tcgtcatatc gaagcgatgg ctaatgcagt agcagacggt gtagatttga tgggctatac
    11521 gccttgggga tgtatcgatc ttgtcagtgc atcaacagga gaaatgagta aacgttacgg
    11581 ctttatctat gtggatctag acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa
    11641 atcttttgat tggtataaag aagtaattgc ctcaaatggc gtaaacttgt aattaaagca
    11701 gctgaatgaa aaagaacaca cttctcacat gtgatttgtg ttcttttttt attttttatg
    11761 tgctattctt ttagtaatag taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta
    11821 tacttggaag tcgcagataa aattaaagaa gatattttct caggaaaata tccagtcggt
    11881 tcgatgttgc cgacagaaag tgaattagaa gaactgtttc aagtcagcaa aatcactgtc
    11941 cgaaaggcaa tcgaactgct ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg
    12001 acgacggttc taagcaatcg tccttacaat cgattatcca aagcggcaag ctttacgcaa
    12061 atcttggaga actcccgata tgaagtccgg aaagaaacgc tggatttgca attggtccat
    12121 ataaagaagg aagatccttt gttttccttt ttcggtgagc aagctgtttg ttttaagcgc
    12181 ttatattatt tgaacaatca gccttatatt tattttgttc attatcttcc acaagaaatg
    12241 atgcaattca gcagagaaga atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa
    12301 ggatatacta ttgactcatt ttctgatgat ttctcggcag tatttttaac agaagaagaa
    12361 caaggaattc tgaaaacaga cgcacagatc ggcatcaaac ggatacggaa atcgtctacg
    12421 caaattgggg aaatgattga atattcggag gcggtctacc atacggagct gcatccttac
    12481 catattgatt atgaaacata aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag
    12541 taataagaac agccaaacaa aaatagcttc tcatattttt cgtttggtta ggcttattac
    12601 cgcagataca cggctgtttc tggattacca tttattctgg ataagggggt gtggcacgat
    12661 tttcagaagg aatctatgaa ccaatcactg tattgatttt agcttcgatg gtaaccccat
    12721 tcggcttgac gatcgcttat ttcttaggga aaatcattcg taaaaatata ctcaatcgtc
    12781 aagaaatcga tacattgaag accgcttttc ctatggggat ctgtcagatc acagagggat
    12841 gtttccctat cgtattgaac gacttggcac gcaatgtcat agcaacggga gtcggtggga
    12901 cagtaggtgg cggattaagc atgttttggg gcgcagatag ccgtattccg gcatcgggaa
    12961 tgtttgcagt cgctacgatg accagaccat gggcattcat tggtgcattg ttagctggtt
    13021 cttttgtcac aggaataacc attttattat tgaaaaaacc agtagatccg aatgcagaaa
    13081 tcatccaaga agaaaaagag gaagaagaca tatcttggga tgatttgaca attagttagg
    13141 agaggcagaa tggattacaa gaaaaaagta caatagatgt accttgagac aggaatggta
    13201 tgaaagaatc agagttagaa agaatcgaat atacagattt tggtttagga gcgatcgaaa
    13261 ctgtccagag catcggcacc ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg
    13321 ttgtcgaaag ggtctggtat accttatgtc gaaggaaagg aaacaaaaaa actttcctgt
    13381 actattccaa aaaggaaaga aagttattat acagtaaaaa aagaaattat tcttcatgct
    13441 agagatcagt acacgttcga gcccaatatc aaacactagt tccaggcagg cgcaacaggt
    13501 gcggttatca ctgaattttc ttccagcagt gacgattcaa gtgatatttt tacaaatcca
    13561 gctatttatc gttaacaaaa agatttttag gagaagctga tgaacaaaaa tgatcaaatt
    13621 ttgatcgttt agttcgtcgg cttttttatt tagagaaaca tcatttatca gtgaaacata
    13681 acacgagaac aaaatgattt tttcgttaat aaaagaaaca agtatttgtt gtttaaaata
    13741 atacacgtta ttggcatata ggacgaaatc ccagtatctt ctataagtaa gtcatttcat
    13801 atcgatgaaa acacgttatt tatttttgtt ttatctataa aatggctgaa aaatgatttt
    13861 tttcta
//
LOCUS       DAYYHX010000059        13811 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000059 DAYYHX010000000
VERSION     DAYYHX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.059"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..552
                     /locus_tag="ACHA6Y_001326"
     CDS             <1..552
                     /locus_tag="ACHA6Y_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8987429.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(705..1178)
                     /locus_tag="ACHA6Y_001327"
     CDS             complement(705..1178)
                     /locus_tag="ACHA6Y_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8987430.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            1316..2788
                     /locus_tag="ACHA6Y_001328"
     CDS             1316..2788
                     /locus_tag="ACHA6Y_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8987431.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2785..3495
                     /locus_tag="ACHA6Y_001329"
     CDS             2785..3495
                     /locus_tag="ACHA6Y_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM8987432.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            3847..4131
                     /locus_tag="ACHA6Y_001330"
     CDS             3847..4131
                     /locus_tag="ACHA6Y_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM8987433.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(4448..4831)
                     /locus_tag="ACHA6Y_001331"
     CDS             complement(4448..4831)
                     /locus_tag="ACHA6Y_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM8987434.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(4833..5417)
                     /locus_tag="ACHA6Y_001332"
     CDS             complement(4833..5417)
                     /locus_tag="ACHA6Y_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM8987435.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(5464..6939)
                     /locus_tag="ACHA6Y_001333"
     CDS             complement(5464..6939)
                     /locus_tag="ACHA6Y_001333"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM8987436.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            7120..7431
                     /locus_tag="ACHA6Y_001334"
     CDS             7120..7431
                     /locus_tag="ACHA6Y_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987437.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(7546..9882)
                     /locus_tag="ACHA6Y_001335"
     CDS             complement(7546..9882)
                     /locus_tag="ACHA6Y_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM8987438.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            10024..10692
                     /locus_tag="ACHA6Y_001336"
     CDS             10024..10692
                     /locus_tag="ACHA6Y_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8987439.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            10676..11737
                     /locus_tag="ACHA6Y_001337"
     CDS             10676..11737
                     /locus_tag="ACHA6Y_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8987440.1"
                     /translation="MRRLFKDEKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRH
                     SISPAESAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFS
                     IRMDEQQKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLL
                     QAPNLSDEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREV
                     IVFFQPKWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFE
                     TATEIVLEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRI
                     DYSSIEGVGTTAMIRLSKQ"
     gene            complement(11974..12855)
                     /locus_tag="ACHA6Y_001338"
     CDS             complement(11974..12855)
                     /locus_tag="ACHA6Y_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8987441.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            13014..13742
                     /locus_tag="ACHA6Y_001339"
     CDS             13014..13742
                     /locus_tag="ACHA6Y_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM8987442.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
BASE COUNT         4178 a         2473 c         2794 g         4366 t
ORIGIN      
        1 atggcaagaa aaaatacact tcgatatatt ttgttagggt tattaagtaa gaagaagatg
       61 acaggatatg aattgaatca atcgttcaaa aatgaaatag gagaattttg gcaagcaaaa
      121 catagtcaga tttatcctga acttgcaaaa atggaagagc aaggaatcat tcagcatcaa
      181 gtagagatca caggtgaaaa attggaaaag aaagtttatg aattaacaga agaagggaag
      241 gaacaattgg cagagtggat tcatacaccc accaatgaac taccggttaa tcgagacgaa
      301 tttgttctga aattgtattt cgtcaaagac gtgaatgatc cggcattagt agaaattatt
      361 cagcagcagc gtgaccttca tgaagaaaaa cgacttcatc tattagagcg aaaaaaaacg
      421 atctttccta ctgaaaaaga aaaacatgaa aattacggac actacctgat tttagcgcat
      481 gcgatcaatc gggaaacaga atatgcaaat tggctaacag atgtattaga agaaagtaac
      541 cgaaaaaaat aaaaaaatat ccgaacagtg atggagaatt cctttctata tgaaagaaac
      601 gcagatcacc gttcggatat tcttatgttc aaacaggcct tttttcaagt atttatgtag
      661 catctactta ttaaaaagct tggtacttag gagattagtg gcgtttagag ttcttttaac
      721 aactgttcgt agtcagcttt ttcatcgaca ggaaggagct tgtaagttaa ttcgtctgtc
      781 tcgttgtcat gaatgatgta catcttagca ttctcttctt tgaagttacc gatttgtaaa
      841 atcgtatagc gttcgtcgat caattcgata tctttgattg cttttgaaaa aactttacgg
      901 acatggtgca ttttcttttg gcggatttct gccttttctg cagcttttga caaagtaaat
      961 ccgtcatcta aatatttttt gatcagttct acttttatga ctgtaggtaa acgatatttt
     1021 ctaggacaat tcgcatcagc ttcgatcgaa cgaatgaatc ccttttgttc ccaatacctt
     1081 aattgacgtg gagacacacc tacgatatca cttaattcac tgatgccaac aaccaattga
     1141 tcattttcca acaaacgttt aagggaagcc ccaaccattt ttgtcactcc ttttgtcctc
     1201 ttgcttccct taattatatc cgtattaact tatgtgtcaa gtaggttgac aaatagcgaa
     1261 tttaggacta aagtattcta gtagtttcag aaaaaattca gaaagaagga gaaatatgag
     1321 taacaaccaa cccgtagata tttacggaaa accatataat cggacacttt tagttgttgt
     1381 cttattaatt ggtacatttt gtaccgtttt gaatcagacc ttattgacga cagcttttcc
     1441 tacattgatg aaagctttcg atatttcagc gtctgatgtg caatggttaa cgactggttt
     1501 cttgctagtc aacgggatca tgatcccaat aacagcatgg ctgatcaata aattcagttc
     1561 aagaaattta tatctttctg ccatgacgat ctttttgatc ggaacgatta cttgctttac
     1621 agcacctaat tttagtacat tactaatcgg tcgtttgatc caagcttgcg gtgtgggagt
     1681 atcgatgcca ttattgcaaa atatcatgct atctattttc ccgccagaaa aaagaggatc
     1741 agcaatgggg atgtcaggga tcgttattgg acttgctcct gctttaggcc ctacattatc
     1801 tggttatatc attgataact accattggcg tgacttgttt ggtatggtac tgccaatcgt
     1861 tgttcttgtc ttggttttag cattcttcct aatgaaaagt gttattccat tgtcaaatcc
     1921 tagcattgat attttatccg ctattttatc aacgattgga ttcggtagtt tactttacgg
     1981 attttctagt gtaggagatg acggctgggg aagcttgaaa gtaattggtt tcttagttgt
     2041 tggtgtgatt ttcatcgcct tgttctcatg gcgtcaatta catttggaac atccattctt
     2101 ggaacttcgt gtattcaaat catctacatt cacgattgca gctgttttag ctggtgtaac
     2161 aaatatggcg atggtagggg cagaaatggt cgtaccattg tatattcaaa atattcgtgg
     2221 agaatcagca ttccattctg gcttgatgtt gttgccgggt gcattgatca tgggggctat
     2281 gatgcctgtg acgggtgcga ttttcgataa atacggcgcg aaacgtttag ccatcacagg
     2341 gatgttcatt ttgacagcgg caactgcacc atttgctttc ttgacaaaaa cgactccagt
     2401 tttatatatc gtgatccttt atgcgatccg tatgtttggg atctccatgg tcatgatgcc
     2461 ggtaacgact tctggtatga atgcattgcc aaataacttg atcagtcatg ggacagcggt
     2521 aaataataca ttccgtcaaa tcgcaagctc gattggtaca gctattttga tcagtgtgtt
     2581 gacgaatgtg acaaaagcaa atatcccaag caaatctgta ttggataata cgccattagc
     2641 ttataaagat caggcaatca atgccacact gtcaggttac catgccgcgt ttatcgtagc
     2701 gatcatcttt gggattcttg gctttattat tgccttttta ttgaagaaaa aagaacctgt
     2761 tctaatgaaa gcgggggata atgcatgatt attggagttt tgattgtcag cttgcttgct
     2821 tttgctttaa cgaacgtgtt cgctaaaaaa acatggcaaa cctttttatc tttgattttc
     2881 ggactaatct tcgtcgcttc actaagtttg atcgtcgcta atttgtccaa tcactttgga
     2941 atggaaaaag taacggaaac aaaaacagaa aaaattgttt ccagtgctga ttcccaagga
     3001 gcagatatgc ttttgtataa agctttaggg aatggaaaag aaaaagtgta tctttaccga
     3061 acaaatgaaa aacaagaaaa accaaaagca actggtacgg ataacgaaac aaataaagta
     3121 gaaaagacag acggcgatgc tgaaaaagtg acgaaaacta cttattggga atacaaaaac
     3181 gacatgtata aattctggtt caatattgct gataacaatc acgaatatga cagccgagtg
     3241 aatacattca aaatcccaga gacatgggta gaattaagta cggaccaagc agccaagtta
     3301 gctgaattag tgaagaaaca gcaaagtacg atggaaagtg aagcaaaagc ttatgttcaa
     3361 gacggtatgg taaaagcaat gactgaaaat ccaaaaatga gcaaagcaga gcaagaacaa
     3421 cgtacgaaag ccttagctgc tgaattccaa caacaagcat tcgcaaaatt agtgaaagaa
     3481 gcaaaaggcg aataatattt tgaacaccat gtaggaggtt gtgaaaaagc tgctctgcat
     3541 caagcagtaa gaacaatcag ataaaaatag cactccatat ttttcatctg attgggctta
     3601 gtgccgtaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgagc ctgccgttta
     3661 actctgagtc acaaggagca atttcaaaaa acagcttctc atgtttgttg actattgtga
     3721 cttgtgaccg aggagttggc aggagaacac cgtttattcg aaaaaaggaa ctgcgacaag
     3781 acttttgtca cagttccttc ttttttttgc tatgctatac tagatgtaaa aaataggaga
     3841 aataaaatga gggaaattta ttcttttgaa tcaaaaatct atgcttccga agtcggcaag
     3901 ggcggggcat atattatttt tccgtatgat ataagaaaag aattcggtgc tggaagagtt
     3961 aaagtccaag caacttttga caatgtgccg tatgaaggca gtatcgtgaa tatgggcgtg
     4021 aaaaatccag atggttcagt ctgctacatc cttggactac gaaaagatat tcgtaaaaac
     4081 atcggaaaag aaatcggtga tattgtagca gttacagtga agcaaaaata aaagaggttg
     4141 tgacaaaaga cgtgtcacaa cctcttgata aacggtgttc aaaagccagt ttctcggtag
     4201 taagtcaaat ttatgcaaaa atgtggaaac ccatgtttac aaaatttttc ttactactcg
     4261 aaacgaagcg gctttatcac aacctcttga taaacggcgt tcaatcagct gtccctcggt
     4321 attagctcaa aaacggcaaa atatgagaag ctatttttcc atttttttct ctcatactca
     4381 ggtcataacg ctgatttcac aacctcttct ttcataatgg tttttcacca tctttataat
     4441 aaactggtta tttgatatgg aaatctattt tttccaacca atctaaaatc ttgtaaccta
     4501 actcttctga catcggaaaa gaggcgttga tttgtgcatt ggagatatgg tcaacatgga
     4561 taccggaagt gatcgtacaa cttcccggaa cggcagtttt gatgatcaca gctatttttt
     4621 cggctaagac atcgtcttta tggaaccgac catcgtggct agggaaacgt acagtttgca
     4681 tctctgtatc acgagaaaat gtagtgactg ttccgatatg tggtgtatct ccgcctgtta
     4741 aagtgatcaa taagtcttta ccgattactt taacagcagc ttggatcgtg aaacctagtt
     4801 tggttacttc aactatcgtt tctttttcca tattaatcac cctgccaacg ttttgaatgc
     4861 tcatttacaa tttcaaattg atccaaaagt ttcattgtat ggtgattaac aagatcttgg
     4921 atcgtttgag gctgattata aaaagcagga attggtggta tgatttgcac acctagtcgt
     4981 gctaatttag tcaagttttc caagtgaatg gcatttaatg gcgtttcacg aggaacaatg
     5041 actaactttc gttgctcctt cattgttaca tcagcagcac gtgcaatcag gttgtcgcct
     5101 aaaccaacag agatacctgc gactgttttc atacttgccg gtacgatgat cattccatcg
     5161 gtcaaaaatg atccgctggc gacagcagca cctaaatctt tgtttgaata atggtaatca
     5221 aacaggcttt cgaattcagt tagtgataag tcggattcca gctttaagtt ctgttttgcc
     5281 catggactga tgattccgtg tgtttctatc atcggatatt cttttagttt cttcactagg
     5341 ttgatggcat aaatggtgcc tgaagcgcca cttacaccaa tcacgatttt tttcttccgt
     5401 tgtgccaatt gttccatttt ccgcactcct ttttatgatg tcaaaacagt tgcagatcat
     5461 tcattatttc aaatattttt cccaatcttc tacttcttgg aattttgcac gtacaaaatg
     5521 ttctttcata tcgaaaggaa ctgtaccgtc aaagatagtt tttgcactta tcccacggaa
     5581 acgaatagag ccaggattat attctggtaa ttctgatggg tccaatggat ggttcctcat
     5641 tcctgggatc acgatgatat ctttatctcc ttggaaacga gtgttgaaag tccaaactac
     5701 atcgttcatg tcgaaaatat caacatcttc atcgactaaa atgactgtct tcaattcttt
     5761 gaaggctgat aaagcaagaa tagctgcttg acgctgaata ccttcatccg cttcgttttc
     5821 tttacggatt tgcatgatgg tcatcaattt accaccacct gcaggaggat tgtagacatt
     5881 tactactttt cctggaatag ctttatcgac caaggcaaga atacttgctt ctgtaggaat
     5941 accggccata gatacgtgtt cttcactggg accaatcgtt gtttgcatga ttggattgtc
     6001 ttttcggtga gtaactgctt tgactttcac tacttgtaca gctggattag catccccagt
     6061 atagccagga aattctggca ttgcttttcc tgagttcgtg ttgatatctt cttgcattgt
     6121 ttcatttggc atgatttctg cttcaatgat gaattcagca cgagcaatac ccacttcatc
     6181 gactgctata ccatcaacga tttgaatagg ttcgttacgc aaagctcctg cacaccatag
     6241 ttcattgtag ccaagtggag tggtaggtgg ttcgaaagtc gtgccaatgg caattgctgg
     6301 atctaaacca atattgatag tgataggcat tggtttattc aaggcttcga attgtttttg
     6361 gaagtgacca atgtggcgtc cgccaggcat gatgtacata ccgatcgtat ctttatcttc
     6421 tagaaccata cgatggatcg ttacatcgct cattgttttt tctggatctg atcctaagac
     6481 tacacccatt gtgatgtatg gaccagcatc ttcaggagta tttgtaggtg caggcaaaat
     6541 gttgcgaata tcgaagtctg catcggtagc tagatgaacg acttcttgtg ctggagcgtc
     6601 tgctttgtct actttcacag gtttgattgg attttctaca gcgtctttca agaaacgacc
     6661 aagtgttcga taatcatggt gcaaaatttc acctacacgt ttacgactag ccattgttcc
     6721 gatgttcaca cgtgtgccgg ggaaaccttt gatattgttg aaagtcatag ctggtccttc
     6781 ttgagtcgga cgttttactg taccgccagc accgatataa cggtaaacac cagataattc
     6841 tgcttcagga tcgatttcaa tatctgtttc gtgatattga cctggaagtg tttttagttc
     6901 ttcgatgact tttcttaaat catacggttg atctgtcatg agagattcct ccttagttgt
     6961 ctttacctat ctatagtaaa aggtgaaaaa gaggaaaaca attcaacttg ttcggtaaag
     7021 tattctacaa tggaatggat gcgttttttt gataatagaa taaactatga tatacttatc
     7081 actaaaagaa agcgcatact tagtgttgga ggaaggaaag tgaaaaaaat ggaggagtca
     7141 tttcctaaag cagtaaaagt ggaaaatata gcgaatattt tgaaagttac atttgaaaac
     7201 ggagaagtaa aatacgtgaa atctcattgg acagaagaga taaccgatgc cttgcaattt
     7261 ggaaaaaaag gaagaggaaa aagaaaaaat cttctcgcac tttctcgtaa tatgtggatt
     7321 gggactgaag taacgattga agcagatgga acagtattca taaatggaaa agacaggtat
     7381 acgccagaag agttgtggta caaaggaaag aaaagtatac cagaattata aaaaataatt
     7441 aaaaaaaaca gcgcgaaaca aggaacatat tacttcctcg tttcttgctg ttttctacta
     7501 tttcagcgct cttatgactc aaatctaaat gtggttgtca caaccttatt ctgcacgcaa
     7561 agcgatagcg gcgtcttttt tcgctgccat tcgtgcagga agatgaccgc caagcatcgt
     7621 caatatagta gagacgatca ctagaataat cgcatggaca ggatttagtt gagcaacatt
     7681 ttttagatcc gtcatgttgt ataagatgct attgataggg aacgtagcta gccatgcaat
     7741 aaacacacct aatatcccag aagaaatacc taaaatacac gtttctgcgt caaatacgcg
     7801 agtgatgtct tttttacgtg cacctaaagc ttttagtaca ccgatttctt ttgtccgttc
     7861 aatcacagat gtataagtaa tgatactgat catgatcata cttgttacta aagagatccc
     7921 tgcaaaagca ataaggacat aagtgatagc atccatcaat ccgccagtca gttcagtcat
     7981 cgttccagct aaatcagtat aaatgatttt atcttcatct gatttgcctt tattgtaatc
     8041 atctagataa tccaagatct tttctttgtc ttcaaaattg ttcggataaa tcatgatact
     8101 tgaaggcaag ctgtctccac ctaaataact aagcaatgtt tgtttggcat tttcgtcaac
     8161 tttttcagta gttaagacat ttacaccact gtctctttga gctttgacga tttctgattc
     8221 tttgttttcg ttgacgattt gagtcgttaa ttgatcgcta taagcgattc ctggagataa
     8281 gagattcatt gtagaagaag atttcacgcg taggatcccc gagatcttca attcatcaga
     8341 agcattctga tacatcgcat cataatctgt attagggata aaattcccag ttggaagttt
     8401 tgtatagtaa gtattgttgt ttactaattt gaatgttgta ccaacgatat catcaaaatc
     8461 aagtttttcg tcttctttca catcaaatcc aagattcttc aatgcgttga tattagtatt
     8521 attattgccg tctacgatca gcacgacatc tgttgctgag gctggataac ttccggcaag
     8581 taaagaataa ttgtctttta aaaagttttc tttgctagtg tcaagctgtg tagggaaact
     8641 tgacactccc acacctgtca ttgctgacat cgtactagat aaagaaagag attccgtatc
     8701 aggattttga ttcgagaagc tgacaggctg tactttgcca tttacatcac gtaaaagatt
     8761 gattcctgta gtacgggtaa atccgatatt attacttaag ttaggatcga tgtctgttac
     8821 gtaatcgacg tatgtttgat caatcttatt cgtgtgctgt gctttatctt cttcgctgac
     8881 ttttgcagta atcgttttgc tgtctggata atccgctttg cttgcaccca atccggcatc
     8941 gtcacgagtc tggttagtcg ttaccttaga aatagtaatc ggaaatttcg ccatcgtttc
     9001 agcttgcgtg ttatcgattt gtttttggaa tccagaagaa agggacagaa cgatcgcgat
     9061 tccgatgatc ccaatactgg aagcaaagga ggtcaagaaa gtacgtcctt tttttgttcg
     9121 gatattgttg aaggaaagct ttaacgctgt ccaaaaactc atctttgttc tgcgtaagtt
     9181 aaattgatca tcttttggtc gttcgatgtg aggatgggaa tctgttaaga tcttaccatc
     9241 agaaaattca atgattcgat ctgcgtattg atgggccagt tcaggattat gtgtgaccat
     9301 gatcactaat ttttctttcg ataactcttg gatcaattcc atgatctgga tacttgtttc
     9361 tgtatctaac gctccggttg gttcatcgca caaaaggata tctgggtcat tggctagtgc
     9421 tcgagcgatg gccactcgct gcatttgtcc accagataac tgatttggct ttttatgcat
     9481 atgatttgtc aagccgactc ggtgcaatgc atcttctgcc cgttttcgtt tttcgtcttt
     9541 agaaacacca cttaatgtca tcccaagttc aacgttttca ataatgccta gatgaccaat
     9601 caagttgtaa ctttgaaaaa tgaaaccgat agagttgttt cggtaggcat cccagtcact
     9661 gtccttaaag tctttagtag actttccgtt gattaccata tcaccggaat cataattatc
     9721 tagtccacca atcacattca gcatagtcgt tttaccagaa ccactaggac ctaaaatcgc
     9781 tacgaattct ttttgtctga aagcgacaga cacaccatcc aatgctttgg tggtggtttc
     9841 gccgacttta taatattttt ttatatcttt taattgaagc ataatcaccc ttctcccgta
     9901 ttgaaatatt cggtatacta ataagtatat cagaacaatg atcgtagtat acgaaataaa
     9961 tgttaactga agatgaacta caagaaaaaa tctagaaatt tttcaaaaaa cagagggaga
    10021 tcaatgaatc gtattttagt agtagaagat gatccgcaat tacagttgct ttaccgttct
    10081 gtgttggaac gagcgggctt ttcagtcatc accgcattga atggagagga tgcgctgaaa
    10141 aaactagaag cgacacgtat agaattaatc attacagata tcatgatgcc gaaaatggat
    10201 ggctatgact tgctagactc attacgtagc accagatttg acacacctgt tctaatgatt
    10261 acggcaaagg ccgattttga ggataagaaa cgcgggttca aattaggagc agatgattat
    10321 atgacaaaac ccatcgatgt caatgagatg atcctacgtg tggaagcatt gcttcgacgt
    10381 tcaaagatca attacagcag tagcttgata atcggacaaa caaaattaga gctagatgct
    10441 tatacagtgt tacaagatca gcattcgttg attctgcctc aaaaagaatt tctgcttctg
    10501 tataaattac tgtcttatcc caacaagatc ttcactcgcc aacagttgat ggatgatatt
    10561 tggggattag atacgaatac agaagaacga acgatcgatg tacatatcaa acgtttaagg
    10621 acccgttttg aaaagacgga agattttcaa atcatcacag tacggggact aggctataag
    10681 gcggttattt aaagatgaaa aaaagctatt ttcacaatta tggatctatt ttgctagtat
    10741 tgtcttcatt tcgatcttag tgacactgct gtgttttctc ggttttattt tcttattgtc
    10801 tcgacattct atctcgcctg ctgaatcggc tggaaaacca acgttatacc cgttattcgt
    10861 ctttgcgggg ttcagtatgg tcgtgggtac aggaatatcg atttttgttg ggcggcgtat
    10921 cctgcatcca atcagtgcgc tagggacaaa tatgagtctg gtagcaacgg gagatttttc
    10981 gattcgaatg gatgaacagc agaaagtagc agaagtacag caactgtata aagatttcaa
    11041 cgttatggtc caagaactca atagtatcga gacactgagg aatgactttg tgtccagtgt
    11101 atcccatgaa ttcaaaacac cactagcaac aatccaagga tatgttcaat tgttacaagc
    11161 cccaaatctc tctgacgaag aacgccaaat atttttatat cggattatcg aaagtatcac
    11221 gcagctttca caattaacag agaacatctt aaaattgaac aaactggaaa accagcgtat
    11281 ccaactggaa aagaaagagt atcgattaga tgaacaaatc agagaagtaa tcgttttttt
    11341 tcagcctaaa tgggagaaag aacagctaga attggatatc gaacttgctg cagtaaatta
    11401 cacaggcaat gaagagtttt tgtaccaagt gtggctgaat atcatggata acgccataaa
    11461 gtacaatcaa atcaatggac agattcatat caaactattt gagacagcca cagagattgt
    11521 actggaagtg accgattcag gtgtaggaat gaatgaagag accagagatc gaatgtttga
    11581 aaaattttat caaggagata caagtcgtca aatttctggg aatgggctag gactttcact
    11641 ggttaaaaaa attctagaac tgcatgatgg acgtattgat tatagcagta tcgaaggagt
    11701 aggaacgaca gcaatgatac ggttaagtaa acaataggaa aaagaataaa acccctaaaa
    11761 attctattga aggtttttag gggtttttag tcgttttttt gtgattaggg aaatgttctt
    11821 acggttcttt tggcataaaa atcgctgctt tcttattgaa ctacgagaaa tgttagaggt
    11881 gtcattgaaa caaggacatg atgcaagaaa gagagcgaaa gtcatgcgtc gctctctttt
    11941 tttgggtgga tagatggaca gaaaatgtag ggcttagttt tcttcttgat ttaaatactc
    12001 ccaaaatgta tctgaagcca aagaatgctc accgacttta cgagtaaatg caagataact
    12061 ctctgttgtt ggagaaatag ggagaatcac agtattttct tttaatagat tcttgactgt
    12121 tttttccata acaatagcaa tccccatgtc atttgcaatc atattgatga tcaagtcgat
    12181 tcttgctcct ttataagaga tttttggttc aaatccagct tcgcgacaca gatctagtac
    12241 aggttgatac aagttcgtag tggggccaag gagcaaaaag ttctcctttt tcagttcgct
    12301 tagatccaat ttctttttac tagctaaagg atgattttta ggtagaactg ctacaaaatc
    12361 gtctgtttca gtaggcaacc actcaatcga acttggtggg gcctggaaac ttctagcaaa
    12421 gtggatcgtg tcactatgtt cattggcgtc attgtaagtt aattcgacac tttccacttc
    12481 tttcaactgg actgtaaatt caggatggcg tttcaaaaac tcggtgattt tggaaaaacc
    12541 tttataattg atcattgtcg gtatcgttaa gatatttaat gaaagattgt tgtctttcgt
    12601 ataggtttca agcacttctt gcatagcatg gtattgttct acgacatttt ttacatatgg
    12661 gagcaataag cttccagctt cggtcaattc gatttttcta tgggcgcgtt cgaataattt
    12721 gacgtctaat tctgtttcta acgaaagaat ctgtttagaa atatttcctt gggtggtgaa
    12781 ttgtcgatca gcagttttgg tgaaattcat tgtttcggct aaatcaagaa aaacttcgaa
    12841 tttatggata tccatcatat tcctcctact tattccaata aagaatactt taacacaaaa
    12901 aatgaattgg tgtaatcttc tctgtctgtt acaatctttc ttgcagcgaa agcaataatt
    12961 acagaaattt ttctgatttt tttcgcttcg tagaaaaaga gaggagtttt ttgatgccaa
    13021 ctggagtttt gatcaatgta ggttccgttt tgtttggcgg actaattggc ggattagtag
    13081 gaaataaatt aagtgaacat tttaaagcac agctgacgat ggtttttggt gtgtgttcga
    13141 tggggatggg aatctattcc attgcgccga tgaaaaatat gccggctgtt atttttgctt
    13201 tagtgatagg aacagcgatt ggtttgattg ttcatttagg taatgggata aataaggggg
    13261 ctgcgctgat gcaagtacct atttctaaaa tcttccctag tgaaaagctt ggtatgaccc
    13321 acgatgaatt tatcagcaca ttagtgacgg tcattgttct attttgtgca agtggtacgg
    13381 ggatctatgg aagtttagat tctgggatga caggagacag tacgattttg atttctaaat
    13441 ctgtccttga ttttttcaca gcagctattt ttgcgtgtaa tttagggtat gtcgtttctg
    13501 tgatagcaat tccccaattc attatttttt atattttgtt cttattggca aaatttattt
    13561 atccattgac tacaccagac atgatcttag attttaaagc ttgcggcgga ttcttgatgg
    13621 tcgctaccgg ttttagaatg atcaacgtga aaatgttccc agtagctgac atgatacctg
    13681 caatgatcgt tatcatgcca ctgagctgga tgtggacaaa ttggattatg ccactgttat
    13741 aataaaaatc aaagagaagc cattttttgc tgactaaaaa ggatcaaatc ttttagtcag
    13801 tttttttgta t
//
LOCUS       DAYYHX010000060        13680 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000060 DAYYHX010000000
VERSION     DAYYHX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.060"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..472
                     /locus_tag="ACHA6Y_001340"
     CDS             134..472
                     /locus_tag="ACHA6Y_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987443.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(677..1239)
                     /locus_tag="ACHA6Y_001341"
                     /pseudo
     CDS             complement(677..1239)
                     /locus_tag="ACHA6Y_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(1971..2552)
                     /locus_tag="ACHA6Y_001342"
     CDS             complement(1971..2552)
                     /locus_tag="ACHA6Y_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8987444.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2735..3361)
                     /locus_tag="ACHA6Y_001343"
     CDS             complement(2735..3361)
                     /locus_tag="ACHA6Y_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8987445.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3383..4714)
                     /locus_tag="ACHA6Y_001344"
     CDS             complement(3383..4714)
                     /locus_tag="ACHA6Y_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM8987446.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4869..5270)
                     /locus_tag="ACHA6Y_001345"
     CDS             complement(4869..5270)
                     /locus_tag="ACHA6Y_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8987447.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5356..5562)
                     /locus_tag="ACHA6Y_001346"
     CDS             complement(5356..5562)
                     /locus_tag="ACHA6Y_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987448.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5657..7159)
                     /locus_tag="ACHA6Y_001347"
     CDS             complement(5657..7159)
                     /locus_tag="ACHA6Y_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM8987449.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7177..7632)
                     /gene="argR"
                     /locus_tag="ACHA6Y_001348"
     CDS             complement(7177..7632)
                     /gene="argR"
                     /locus_tag="ACHA6Y_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8987450.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7730..8347)
                     /locus_tag="ACHA6Y_001349"
     CDS             complement(7730..8347)
                     /locus_tag="ACHA6Y_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8987451.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8568..9941
                     /locus_tag="ACHA6Y_001350"
     CDS             8568..9941
                     /locus_tag="ACHA6Y_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8987452.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10016..11128
                     /locus_tag="ACHA6Y_001351"
     CDS             10016..11128
                     /locus_tag="ACHA6Y_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8987453.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11200..12837)
                     /locus_tag="ACHA6Y_001352"
     CDS             complement(11200..12837)
                     /locus_tag="ACHA6Y_001352"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM8987454.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13005..13613)
                     /locus_tag="ACHA6Y_001353"
     CDS             complement(13005..13613)
                     /locus_tag="ACHA6Y_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8987455.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3686 a         2882 c         2449 g         4663 t
ORIGIN      
        1 aaaaataatc caagaaacat tgaacctatg gatccaaaag ctacccaaat actatagcta
       61 accccccgct accttcaatt ttgcagataa aacgctttgg gagaaaattt caaagacaac
      121 aggccatcct atcatgcccg attataaaca aaaagctggc tattcctttt gctctgacaa
      181 atttcaattg ggaaagttca cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa
      241 agaatgcccg cttcaaatag aaacattggt tcaacgaatt tcaactagac aagactttgc
      301 tattgtagaa cgtgagatcg tagctatttt tgtagaagaa gggattttac atgatccaac
      361 tcatatcaat gtggataaat ggcaacctct tatctataaa ttcagagaat atacaactgc
      421 caaccagtcg ttaactttga attttagatt ccaagaattc aaagagtcat aatgataaac
      481 atcgttaggt ataaataaaa aagactcgca gcacgagtaa aaaacaaata gaagctatac
      541 attagaaagg tcgactgtga ccctatagat gcagtatatg cagcttatga gcatttcttc
      601 tgttctcttt gtttttggga tctgtgctga agtctttttc gttttttgcc gctctttctt
      661 tgaaaaatcg attatgttat accttctcca ttactaattc aggcatctgc tgagtaatga
      721 tttttcgcgc aaaagaaata tctgttaccc aaggttcgat ttgttcttca gtcaagacaa
      781 gcggcattcg gtcatggatt ggtgcgactg tgcggttagg ttcagtagtc attatgattg
      841 attctgccat tttttcatcc tgatggctac gataaaaacc gccaatatac actatttttt
      901 ccttatttga aaacataaat ttgtcttttt cttcattcca ctcattggga aaacacaacg
      961 attttgcaaa aatggttgat gaaaggtctt tttttcttct actgtctctg cacgggcatt
     1021 aatcatcaat tgtccttttt taaatccggt aaatccccac ttcatgattt ccggagtgag
     1081 gatgttttct tcctcttttc ttaacgtcac aacaagattt gacggaaaaa cttctccagc
     1141 tgctaattct gctgcctgct tcggttgttt cgtctgggct tctcgataat aattctgaag
     1201 gagctctgac tctagatcaa aaaaataacg accacacatc ctaatccctc ccaatctttt
     1261 tttctatcat acactgcaac aagaagttcg caccaacaaa acgctttcaa tttttgtaga
     1321 actaaaaaag ttgtgacaag tgacaaactc tccagtgaat ggtattctcc tgccaactcc
     1381 tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg
     1441 tgactcggag tgaaacggca ggcttacgac ctctgggtaa acggtgttca aaagccagtt
     1501 tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc tattttcaca aaatttttct
     1561 taatactcga aactaagcga ctttttcaca acctcttcct taaacggtgt tcaaaagctt
     1621 gcatctgcgg taataagccc aaccaaacga aaaatatgag cagctatttt tatttggctg
     1681 ttcttattac ttgatgcagg gcagcttttt cacaacctct ccactaaacg gtgttcaatc
     1741 agctaacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg
     1801 tttctctaat actcaggtca aagcgctgat ttcacaacct ctaatacacg gtgttccaga
     1861 agtagcttct tcgaaaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa
     1921 attccttctt atttcttgaa gctgaccact tctgtcacaa cctctaatta ttaaagaacc
     1981 ttttgcgaag ttaacaatct ttctttgatc aattgaaaat tctgcctaag tagtgataga
     2041 tcctgtgtga ctttcaacaa taagatactt ccctggattt cagcaatcaa cgctttggct
     2101 tgacggtctg cttcttcatc ggacagtttt tcttcagaaa gtgccaggat attttgtttc
     2161 gtatacttga cccattgatt gactaattct agtagcaaca gacggtattc ttcatccact
     2221 gtactcaact caataatcaa attacctaca ggacagccat gataagtgat ctgtgtctca
     2281 tgatcagcga aaatccagtc caacatttct tctaatgctt cttctggctg ttttccagca
     2341 tttaagatgt cttcgaacaa ttctgtctgc catacgtcca tatgcgcatg gatcaccgct
     2401 agacaaactt ctttttttga agaaaagtaa taataaaatt gaccttttcc tgtatcagaa
     2461 gctgctagaa tatctcggat tgacgtttca tgatagcctt ttttataaat aagatctttg
     2521 gtcgcttcca agatcttttc ctttgttttc aatgatattc tcctttttca tttctcatag
     2581 tcctatatta tacagtaaat tcatcatttt ttcttcttaa tcgtttaaaa aagactgtgc
     2641 caaattatct gacacagccc tcactataaa actgttctca tatagctaga tgttcattga
     2701 ttcaagaact gcgaggacaa aagcaattcc tatcttatga tagttgctct aatacttcct
     2761 ctgttgtcat cacttgatct gcaataaagc caaagttcgt caacgctgca tccattgctt
     2821 cttggccagg agcaccaatt gcatctgtcg ctaccacaac attgaatcca ctctctacca
     2881 attcacgcat atgggaatcg ctacacaagt ttccgttcat tccaccaaga attactgtgt
     2941 cgattccatt ttttctcaat tggagcgcca aatcattgct ttctggtcca aagattttat
     3001 gaggagagct gatgatcgta ttttcatcca aataaggttt caattcttcg ataaaatcgg
     3061 ctcctgaacc ttcttccact gattcatatt gactttttct agcaaacatc tgctgctcaa
     3121 gcatcatatg ttctcctgca ccgccaaatt gccatttatg gtcatgtggg taaaaataat
     3181 gaggagaaat aaagagtttg tatccttttt cttttgcttg ttttaccatt tcaatcatat
     3241 tttcgatcgt attgtatttt tctaagatat cttttgtcaa accaaagcca ccgcctgctg
     3301 gtttcaagaa ctcgacttgc gggtcagtga gaactactgc tgtattttta ttattgaaca
     3361 tggtggattc tccattcgtt atttatttta atacatcatt tagagcttca ctcattgtcg
     3421 gatgagaata gatttgatct ctcaataacg tataaggaag ttttccattc atagctagcg
     3481 aaatcaagtt gatgacttca tgagaatctt ccgcatagat acttgcccct agaatcaagt
     3541 cagtttcagg gtcgactaaa actttgaata cacctttagg atcttctaat acttgtgctt
     3601 tagggacacc gcttgtcata tacttgaata atttgtaatt gacgccttgt gctttggctt
     3661 gtttttctgt taaacctaca ttggataaag tcggagtgat aaatacactg taaggcacag
     3721 ctttccggtt attcgttgtt cgtttattct cgcccttcaa ctgatccata atgattcgat
     3781 aatcatctag agagatataa gtgaactgca atccgccttt gacgtctcca atcgcccaaa
     3841 tgttgtctgc atctgtacga aggtattcat ccacagcgat tgctccgcga tctgttagtt
     3901 taacatctgt attttctaat ccaagtgatg ctgtacttgg ttttcttcca gttgcagcca
     3961 aaacttttga cgcttggatt gttacttctt ggccatcttc tgtaaaggtg acggcagctg
     4021 ttgattcttg gtctgctact tgatctacag atacacctag atggaattcg attcccgcat
     4081 ttgtcatgtc ttctaaaatc atttgcgcga tatcttcatc ttcacgtgga agaaattcag
     4141 aatgggcatc aagaacgaca actttagatc cgtattcatt gaacattgat gcaaattcca
     4201 agccaatata tccagccccg atgatcacta atttttccgg taattgttcc tgatccatcg
     4261 cttgcgtaga atcaatcaag tagcgacttg tattcaatcc tttgattggt aaaatgattg
     4321 gttcagaacc agtattgatg aaaatccgtt cgccttcaac aatcactttt tcaccattat
     4381 tcgtcacttc gatttgatgg ttagaaagaa aacgtgctgt accgtccatc actgtcccag
     4441 ttgcctcatc gctgatcata tgataatttt tggcgcgaag catgcccgtc aatttttctt
     4501 tccgagcaac agcctctgta aagtcgattc ctctttctcc attgaaaatc aatgatttag
     4561 aaggaataca gccgatgttg atacatgttc cgccatacat acgtgttgac ttttcaacga
     4621 ctaaaaccga ttcacctttt gaagctaacg ccttcgctaa agtcttaccg ccctttccaa
     4681 aaccgatcac aatattttga tatttttcca tcatttttcc ctcgcttctt ttttacctac
     4741 cgattggtac aaaaatatga taaccgataa ttgtctattc tccaaatcat ctgctttatt
     4801 tgtcaaaaaa aaccagacaa aaagcggtcc tttcacgttt ttagtctggt tcgatttcct
     4861 ttattcttct aaacaatcgt tacttctaca tcgatatttc ctctagttgc tttggagtaa
     4921 gggcagaatt catgcgcatc ttctgcaatt ttttgggctt cttcttcgct gatgccatcg
     4981 atatgcactt ctaatactac accgatctga tagccgccat catctaaatt ataaagagag
     5041 acacgtgctt ttactgtgga ctctgctgtc attcctgctt ctttgatcat atgttgtacc
     5101 gccccattga aacaagaagc ataacctgct gcaaataatt gttctggatt tgttccttct
     5161 gcatggattc ctggaggagt gattttcata tgcatcgagc catttggtgc ctcgacttct
     5221 ccattgcgcc cgccatggtt gatcattgct gtttcgtatg cttttttcat tgtactgctc
     5281 ctttcgattg accatttgct acttggctat aattatagtg tatgacaagt aaggcgcttt
     5341 ctacaaaaat tctgcttatt cgaataccag aatgccgtct tctactattt ttccaaaaaa
     5401 caaaaaaata ctactcgtga acaacagcgt catatttgtt ccaaaataaa tagcaatctt
     5461 ttctacataa tcttttatat gattgatttt aagatacata tataccagaa agttgacccc
     5521 ataaaccaat actgttaaaa aaataaccat attcgttacc attttttcct ccttttgctg
     5581 gtctccctac cagcactcga tttaaccata ccactttttt tgccgtccaa gcaactagcc
     5641 tttcaaaaag actggtttat tctgctgtta atgattcaag atcagaaaaa tgtccgaata
     5701 attttgctaa ttttactaac gccaacttgt tctttccgaa ttcatcattt tctatctgta
     5761 atactagtaa cggctcatgt aagcttaaac ggatcaaaaa ccagccgttt ccgtatggct
     5821 cttctagatc atatcgaata ccttcttcat tttccgggtc gaagttcatc cctttttctt
     5881 cttccaacag tcgcttcatt tcttctaata tcttgtttcc ttgttcgcgt ggttcatcgc
     5941 tgaacaattt taatcggatt tcttgtactt ctacgggctg tttcaactct ttgatttgtt
     6001 catctaatgt gatacctttt ctttttaatt ctggcaaaag catcaggatc tttgcgatga
     6061 catatgcacc atcatccaga aaatagtttt ccttgaatgc agcgtgtccg cttgtttcga
     6121 tggctagctg tgtatctttc ccaagacggt taagttctaa tgctttattg attacatttc
     6181 gatagccgga aatataacga acttggatgc ctcctttttc ttcaatgaat ctttgtagat
     6241 gagaagaagt aggggaattc gtcacgattc ttgttcctgg atgctctttt aatacgattg
     6301 tacttaatac tgcgatcaaa ttatttcgat tgatcactgt tccagaagca ctgacaactg
     6361 cagaacgatc cacatctgta tcgaaaatga tcccaagatc tgcttcattt gctaaaacag
     6421 cctgcttgat actttccatc gcttctttat tatcaggatt tgggatatga ttaggaaatc
     6481 ggccatctgg ttccaaaaat tgggaacctg ttgaatccgc acctaagcta ttcaatactt
     6541 tttcagcaaa gaatccacca gcaccatttc cggcatcaac aatgattttc caaccattta
     6601 gtggtttgct ttctagtgtt ccaatcccct ttttaatttt tgtgactaaa tcttgcgcat
     6661 aaggtgtcaa aaggtctgct ttttctacgc ttccagtttg ttcgcttttt attttttgct
     6721 gctgtgatag gataaaagca atatcttctt tttctgctcc tccttctgag gtaaagaatt
     6781 ttattccatt ataataatag ggaagatggc tagcagtcag cataaccgca gcatcgcacg
     6841 agaactgttc aaattgagtg gacataaaca tagcaggcgt agtagctaat tcgaaatcga
     6901 tgacatgtat cccttgttct ttcagacttt ggatcaacgc ctctttcagt gctggtccgc
     6961 ttatcctact gtcatgacca atcccaatcg tccatttttt atttggatcg gttgatttct
     7021 tctttttctt tatccagttt gctagtcccc agccgatctt ttttaccatg tctgtcgtca
     7081 agtttacttc atatccttca actggtaatg cgattcctcg aatatccgat ccattttgca
     7141 gcgctttcat atctttcata taaaagtcct cctcatttat tccctcattt tttccagctt
     7201 ttctgcaaat cgctgtgctt cttcttctga gcggcaaata ataatgaatg tatctacacc
     7261 ggccatcgtt ccgacaactt cagggtaagc cacttcatct agccaattcg ttaccacatc
     7321 ggcaccattt cgttcagtca atacgataac catgaactga acagactgga tacgcaaaac
     7381 ctcgcgtcta attgctgtac gcaaacgttc ttcatttaaa gaaacagatg gctggttaaa
     7441 taatgtatag cgtactattt tgttttcatt ttgacttttt actaacttca tttctcgaat
     7501 gtctcgagaa atcgttgctt gagtcacatc ggcgcctgct tcatgcagcc gcgcaagcag
     7561 ctcttcctgt gtcccaatag gttgttctgt aatgatctgg cggatcagtg cttgtcgctg
     7621 tttctttttc atattagaca cctctcaatg ttgattatag caaaagataa gtaatatttc
     7681 ttcatatttt ggtatttttt acttttattt tttgttttca ttcaggttgc taaccttttt
     7741 ctattttaac acgcaaaatc gtcttcattt tttcaggtgc tgattttctt ggatcgctga
     7801 tgtaaatctc tttgtgttct ttagaagctc tttcatagcc ttcttttgcg atgaatgctt
     7861 ctaatttctc aaacgttgct ggttcttcgg catagggccc gacgtgtaag atttgggcac
     7921 aaagaccttc ttccatcttt ttgaatacta cttgttcttt caagtcttcc ggaagtttgc
     7981 tagaacgctg aatggcttct tgtgctatcg cttctgtaac aaagtcaggc tgtcggatca
     8041 tcaattgata agaaaaatgt tcttttttca tcactggttc atgcaaatac ttttcttgca
     8101 gtccccattg tccttccagt ggatagacag tatacatctg ataatcagga atcagccaat
     8161 tttttttagg cgacatgcgt attgcatagc tgactgcata tagacaggct actcttcgtt
     8221 taaagtcttc ctgattcggg tcgccgatcc ctctgatgct aaagaatgat tttttaggaa
     8281 cctctagtat ttgcgggctt tttttcatac tatagctctt gtctttacgc cattcaattt
     8341 tttccatttt tcttcctccg cgaaattcca tttagttatt tattatcata cctctttttg
     8401 agtgacttgg ctaatcgtgt tgacactatt tttcagtttg tgctagaata agtgaagtaa
     8461 ataaatcgta cagataacac gtttcggcgt gtcgagagag aggcggtttc tatttttata
     8521 atagaaatca ctctcttttt ttacgaaatt ttttagaggt gatgtaaatg acaaaacaaa
     8581 aacagctgct tttagtacct gcagtaattg caacagggat gatgtctttt gcgggcgtat
     8641 tgatcgagac ggccatgaat gtaactttcc caactcttat cagacagttt gatttaacga
     8701 ctgcacaagt ccaatgggta acaacgattt atcttttgat gatttcgatc attgttccgc
     8761 tgtctactta tttgaatcgc aattttagtc tcaaaaaact ctttttagct tcaaatctgc
     8821 ttttcttggc aggtataatg atcgatttct tctctcctac cttcatggtc ctattgtttg
     8881 gtcgattgct ccaaggtgca gcgacaggta tcgctcttcc gttgatgttc catattatct
     8941 tgacatttgc ccccctcaat cgacgaggcg cgatgatggg tgtcggtact ttgacaacgg
     9001 ccattgctcc agcaattggc cccacttatg gaggcattat gacttcacat tttacatgga
     9061 atcatatctt cctgtttctt gtgcccgttt tgtttctttc tttgattatc ggctggtttg
     9121 ctattcccga gatggcagta caaaaaagtg gttatttgga ttggataagc gttttaggaa
     9181 tttgtctttt gtttagtgga agtctaacat ttttgagtac gttgggaaca gtttccagct
     9241 ggattgcttt aatcatcggc ggtattggct ggttcctttt ttactatcga agtaaaaaag
     9301 tggagcatcc actgatccga ctgtctattc tttcaaataa aacattccgc ttgtttttga
     9361 ttggcttctt agtttatcag tttttattac ttggtgtatc ttttgtcttg ccgaatttcc
     9421 ttcagatcgt tcaaggaagt agtccttttg tcgctggatt agcgatgctt cccggcgcag
     9481 cggttggtgc tttgctttct cctttttccg ggaaaatgct ggatcaatat ggtccgaaga
     9541 agccaatttt tgctgggctg attctttctt tgattggttg gctagcatta ttcctactca
     9601 taggacacgc ttcagtgggt gtattgattg cttgtcatgt gttttatatg atcggtatcg
     9661 gattatctta cagcaatatg atgaccaccg gaatgaatgc actgaatgaa gaactacaag
     9721 gagacgggaa cgcaatgttc aataccttgc agcagttttc tggcgctgtg gctacttcat
     9781 tagtagcggt cattatcaat tacgtccagc atcatacttc ccataactat gaagtgtcta
     9841 ctacattagg atcaaaagca gcattaggcg tcttgttgct gttgcttctc gtaagctttg
     9901 ctcgattcgc ctattatctg ttctttgcta aaaaagcgta gattccatat acggcaggtg
     9961 ttgcatctgc cgtttttttg tgtgaaaatg gttgtttgta aaaggaggct tttttatgga
    10021 aaaaattgcc attatcggtg gcgggatcat tggtatgacc ctagccagtt acttagatac
    10081 atcaaaattc aatgttgctt tatttgatga agggacagga caggcaacta aagctagcgc
    10141 tgggattatc tccccttggt tgtcgaaacg cagaaacaaa caatggtatc gtctggctaa
    10201 agacggcgct gcttttttcc ctaaacttat aaaagatttc caacttgatc aaacgattta
    10261 cgcacaatca gggacacttt tgcttcgacc tgaaaaagat ttagctgaat tggctgttct
    10321 tgccgagaaa cgaaaagaag aagcgccaga aattggaaac atcgaatggc tttcacctga
    10381 acagacatct caagcacttc cgttattgaa accagcagtt tcactgcgta tcagtggtgg
    10441 tggtcgtctg gatggaaaag catatttaga acatcttggt cagcgtttag cacaaaaaca
    10501 aatggagcag atccaggcaa aagtcacttt gcataagaag agcgattctt ggctcatcaa
    10561 gggaccgttt ggagaataca cagctgatcg tgtaattttg acacctggcc cggcacttaa
    10621 gcctttatta caagaattag gctatgctgc agatatacgt ccgcaaaaag ggcagctctt
    10681 agcatttcag acatcttata agatgagtca agactggcct gttgctatgc ttgacggaga
    10741 agctgatttc attcctttca tggatggaaa gatcctttta ggtgctaccc atgaaaacga
    10801 tcaaggctgg gatttggctc ctactagcga agcttataag ggattgacta agagtacaga
    10861 acagttcttg actgatccag atcagataaa aaactggcca tacgagttcc gtgtaggtac
    10921 ccgtgcttat acgtcagatt tttccccatt tttcggcgta ctgccagatg atccaagctt
    10981 actggctgct agcggtctag gctcttctgg cttgaccaca ggtccttata tcggctatct
    11041 attagccaac ttcttaaata ctggtcattg gcctacggaa gattatcaaa aggacatctc
    11101 cacctatatt acgaaaaatc catcatagaa aaacagcttt ttcaagtaag gatcccgagc
    11161 gatattgcgc atggattact tcttgaaaaa gctgttattt tagttcaatt tctgaatcaa
    11221 attctccaaa ctttcttttg cttcatctgg caagagatct tttcctatct gagcttcaat
    11281 gaatcgtaaa cgaatatcaa gtcgttgttt caccagattt ttataggacg tatcctcaaa
    11341 agaaacagga aaatctggta taggtaaata gtggatgtct tcaaatggac caaacgactt
    11401 gaattctgcc gtttgttgca cgattctttc aacgattgac aatgtcgttt caggtttgat
    11461 ttttttatca tcgaaatatc cagtattcag gacatagcag gctgtttgct gtttcaaaaa
    11521 caactctcga aaagcttgat agtcttccct aagaggatag acacggaaag gattagcaaa
    11581 tggttcaatg actagctcat ctagctgtct ttttgtagtg atattttccg cgtctgatcg
    11641 ttttgttgct aatgttgcac caaaagacgc tgctaaaact gggtcattga tcttgataat
    11701 tggcggaagc gaatcatctt tcatgatcca aaaaacagca tcgatctttt cgctaagatg
    11761 atcgactcgg tcgggtgtcg cataacgtga tttgactgtc cgcccatttc cgtttcgtat
    11821 atctcctgtt actaggactt tttctccttt acttgtacat gtcacgccca cattctgaca
    11881 agtaaggaaa gaagaaatgg ccggatcatc agacggatag tcttgtgttt tatcaaagta
    11941 agaaggttcc aaggcagtag caaaaccatt ttttcgagaa ataacgaaag catcgtcatg
    12001 caagatcgaa acagagtgac cttccgtatg ttttgctaag gtaatcgttg attttccaga
    12061 accggacaac ccaaatacag ccatcgtata ggtctggtca ttactagcat attgtttcat
    12121 cccaccgtgg caggcaataa agccattgcg atttcccaat gcccaagcaa gcgtcaaagt
    12181 cgcttttttt aattctccaa agtaacgtaa tcctagcaca gccgctgtat tatggagagc
    12241 atcaaacaaa atgagtccat ttggaaatgt ttcatgtgtc cattcgggat cacaaaagat
    12301 ataaagatct ccctcatcaa aggaaacaga cttttgataa gtctctaaga tcttcgaatt
    12361 cgctggctga aaattcagca aataagaata caaattgttc tcaaatcctt ctggaatcag
    12421 caaatgactt tgcacagaaa attcttcaga taggccaacg attacttttg tatggtaaaa
    12481 ctggcgcttg cgactttgat acacagcttc tcttaaaatt ttctcgtatt tgctttgttc
    12541 cttgtctaag tcctcaaaga tccgccgagc acttgctgtt cttcccacaa tgcttccatc
    12601 attgaatact agtgtcctaa catcagctgg taaatctagt tcttcagcat gtgagattgt
    12661 taaatctgtg atgatcgttc ctggactccc ttttgctagt tgataagctg tcttcctatc
    12721 aaaaacttca tgaacattat taccgtaaaa tgtcgtttct acaatcgatc gaatactgga
    12781 taacagtgga tttccacttg tgatttcgtt taattcgaga ctctcttttg tactcatggc
    12841 tcctcctcat tccctaaaca aaaaaacgtt ttctttatta tacattattc gagtttttat
    12901 ttgtactgct gcgctgtggg aagcaaacga gttatttttc acacaaaaaa accgagaact
    12961 tgaataaaca agtttctcgg tttcatcatt ttccttttat cggcttagta ccagccgttt
    13021 gctaaccaga atgttttagc agcatcccat gaaccataac gagatgttac atattgttct
    13081 gctacacgtt cttgattggc agctgaatag tcgccgttta agtatgaagc atctaattga
    13141 taacggccga tgtaacgtcc gttagtagct gtgtatgaac cgcctgattc tttttgtgcg
    13201 atccattctt ttgcagagct tgaagttggc gctgttgcag cagctggggc agcaggagct
    13261 acttcaactg tttcaacagg tgcaacgtat gtatgttctt gcgttgctgt ttcagttgta
    13321 gatgctgtat tttctgttgt tttgttttct gttgttgctg aagaatcatt tgaagttggg
    13381 attgttaatt gttcaccaac ataaatgcgg ttgatgtttg cgatcgaatt tttttcagca
    13441 attgcatcga tcattgagtt gtctcctgca aatttttgtg agatcttaga taatgaatca
    13501 cctgatttta ctgtataaac ttcatcagcg tgagcatttg tagttcctac aaacattgca
    13561 gtagcaccag cagcaaaagt tgtacctaaa acaattttct taaatgaatt catattaaac
    13621 tccttttttt ctccgttttt tattctttcg acgctattaa ctttatcatt tagaaatatt
//
LOCUS       DAYYHX010000061        13618 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000061 DAYYHX010000000
VERSION     DAYYHX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.061"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..1448
                     /locus_tag="ACHA6Y_001354"
     CDS             21..1448
                     /locus_tag="ACHA6Y_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8987456.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1540..1905)
                     /locus_tag="ACHA6Y_001355"
     CDS             complement(1540..1905)
                     /locus_tag="ACHA6Y_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8987457.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1902..2267)
                     /locus_tag="ACHA6Y_001356"
     CDS             complement(1902..2267)
                     /locus_tag="ACHA6Y_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8987458.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2459..2707)
                     /locus_tag="ACHA6Y_001357"
     CDS             complement(2459..2707)
                     /locus_tag="ACHA6Y_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987459.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2706..2834
                     /locus_tag="ACHA6Y_001358"
     CDS             2706..2834
                     /locus_tag="ACHA6Y_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM8987460.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2891..4585
                     /locus_tag="ACHA6Y_001359"
     CDS             2891..4585
                     /locus_tag="ACHA6Y_001359"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM8987461.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4685..4981
                     /locus_tag="ACHA6Y_001360"
     CDS             4685..4981
                     /locus_tag="ACHA6Y_001360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987462.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5359..6492
                     /locus_tag="ACHA6Y_001361"
     CDS             5359..6492
                     /locus_tag="ACHA6Y_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM8987463.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6523..6843
                     /locus_tag="ACHA6Y_001362"
     CDS             6523..6843
                     /locus_tag="ACHA6Y_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM8987464.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6872..7336)
                     /locus_tag="ACHA6Y_001363"
     CDS             complement(6872..7336)
                     /locus_tag="ACHA6Y_001363"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8987465.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7467..8720)
                     /locus_tag="ACHA6Y_001364"
     CDS             complement(7467..8720)
                     /locus_tag="ACHA6Y_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8987466.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8749..9066)
                     /locus_tag="ACHA6Y_001365"
     CDS             complement(8749..9066)
                     /locus_tag="ACHA6Y_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8987467.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9092..9412)
                     /locus_tag="ACHA6Y_001366"
     CDS             complement(9092..9412)
                     /locus_tag="ACHA6Y_001366"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8987468.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9876..11291)
                     /locus_tag="ACHA6Y_001367"
     CDS             complement(9876..11291)
                     /locus_tag="ACHA6Y_001367"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8987469.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11308..12624)
                     /locus_tag="ACHA6Y_001368"
     CDS             complement(11308..12624)
                     /locus_tag="ACHA6Y_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8987470.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12774..13472)
                     /locus_tag="ACHA6Y_001369"
     CDS             complement(12774..13472)
                     /locus_tag="ACHA6Y_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM8987471.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4315 a         2772 c         2349 g         4182 t
ORIGIN      
        1 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
       61 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
      121 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
      181 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
      241 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
      301 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
      361 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
      421 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
      481 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
      541 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
      601 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
      661 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
      721 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
      781 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
      841 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
      901 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
      961 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
     1021 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
     1081 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
     1141 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
     1201 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
     1261 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
     1321 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
     1381 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
     1441 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
     1501 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
     1561 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
     1621 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
     1681 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
     1741 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
     1801 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
     1861 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
     1921 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
     1981 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
     2041 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
     2101 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
     2161 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
     2221 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
     2281 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
     2341 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
     2401 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
     2461 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
     2521 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
     2581 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
     2641 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
     2701 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
     2761 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
     2821 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
     2881 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
     2941 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
     3001 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
     3061 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
     3121 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
     3181 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
     3241 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
     3301 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
     3361 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
     3421 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
     3481 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
     3541 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
     3601 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
     3661 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
     3721 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
     3781 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
     3841 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
     3901 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
     3961 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
     4021 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctatcgt
     4081 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
     4141 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
     4201 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
     4261 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
     4321 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
     4381 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
     4441 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
     4501 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
     4561 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
     4621 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
     4681 gcagatgcaa gcttttgaac accgtttacc aagaggttgt gaaaaagctg ccctgcatca
     4741 agtaataaga acacccaagc taaaactgct tcttatgttt ttgacttggt tggacttatt
     4801 accgcagatg caagcttttg aacaccattt accaagaggt tatgaaaaag ctgccctgca
     4861 tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt ggttagactt
     4921 attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta
     4981 gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga
     5041 cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt
     5101 tttgaaaaca actatttttt caacgtttac tctcttgcca atccaacatc atctaacgga
     5161 tacaactatt tcaatgatta ccattacact aaaaaatcgg aaacacacct ctccgaacac
     5221 aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg
     5281 taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat
     5341 ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc
     5401 agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca
     5461 acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa
     5521 gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa
     5581 acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat
     5641 ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc
     5701 gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta
     5761 agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa
     5821 aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga
     5881 gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa
     5941 aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt
     6001 ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa
     6061 gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta
     6121 ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc
     6181 ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa
     6241 gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg
     6301 gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgca
     6361 atgattgcag aaggactatt cgatcctaca caaatcatta ctcattcgct gccactatca
     6421 gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt
     6481 ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga
     6541 aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc
     6601 cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg
     6661 attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc
     6721 tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg
     6781 aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca
     6841 tgagaatttt tcaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac
     6901 gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat
     6961 aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt
     7021 ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa
     7081 acagggcaca aagatttggt gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc
     7141 ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt
     7201 ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga actccccatt
     7261 ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg gtgttctctt aatttgatcc
     7321 agttatatgt aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac
     7381 aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa cggaaaacaa
     7441 tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg
     7501 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
     7561 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
     7621 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
     7681 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
     7741 ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag
     7801 tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa
     7861 cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg
     7921 ataaacggtg cgctatgccc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca
     7981 ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag
     8041 aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat
     8101 ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa
     8161 attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat
     8221 gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg
     8281 cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg
     8341 tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct
     8401 gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gaaagcaatc
     8461 aagacagaaa gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc tttccatgat
     8521 tcaccaccaa aaattgagtt catcaattct tggaacccgg gaatcggtaa gttattgatc
     8581 attactgcta atgctcctaa gatcattaac ggcatactca ccataaatga gtcccgaatc
     8641 gcaactaaat gccgttgtgc accaattttt gatgctacag gtataaaatg tttctccata
     8701 aatgcaacaa atttatccat aaatcttccc tcttttcttt actgtagatt acgcgttcaa
     8761 tcgacgatac agatcaatga tctcttttgc taaatctgta aaagctatag atgtcatcag
     8821 atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt gtgcttcttg
     8881 tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa gattcctctg catctttgat
     8941 tttttcatct gctaaagtga aatcaccttt tttcgcagca gcaatcgctt ccattgcatc
     9001 gctttttgca tttccgccaa acatgatcag acccatgatg gcttctagat ttttttgatc
     9061 ttccatttat tgtatcctcc gataattttt attatttgtt tattagtttt tctgcttggt
     9121 cgagtacttt ttcaccattc atcatgccat agtcagccat attgattaca tctaaaggaa
     9181 tgcctttagg tgctagcttt tcagcaaatt gattttgcat aaagcgtact tgcggtccta
     9241 aaagtaaaac gtctacattt ttagattcta gttgattatc cgcatctgaa gcagatacag
     9301 caaagatgtc tgcttccatt ccttttgctt ctgctgcctt ttgcattttt gtgactagga
     9361 gacttgtact cattccagct gaacatacaa gcatgattgt tttcttttcc attttaaaca
     9421 cgttcctttc tatacttaca cttgtatata agcaagtagc gtgccaactt taaaagcgct
     9481 ttaaaaagtt gatatcatca catttaaggt ctataccaag ttgcatacac actaaaaaag
     9541 aaagcgcaca ctaaacacac aaattaattt ttattacctt ttttctttta acgtgcacaa
     9601 aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa taaacggtat tcaaaagctt
     9661 gcatctgcgg taataagtcc aaccaagtta aaaacataag atgttgtttt tactttgcta
     9721 ttcttattac ttgatgcagg acagcttttt cacaacctct caataaacgg tgttcaatca
     9781 gctgaccctg ggtattagct caaaacgctg gattcacaac ctcttttatt tcaaataacc
     9841 agtaaataca atgaacatct acaaaaaact tctatttatt tatagatttc gtcaggcaaa
     9901 tccaattctc tcttatcaat caaagtctga taccacgaag cagatttttt caatgttctg
     9961 cttcgttcat cgttcaattc gattcgaact aatccatatc gattcttaaa tgcattcatt
    10021 ggtgacacac agtcagtgaa tgcccataac atatagcctt cgcaatttgc gccttcttct
    10081 gttgctttga tcaagtaatg caaatgctgt ccgataaatt cgatacggta atcatcttgc
    10141 acgattcctt tctcatccat atactgttct tcatattctc ttcccatacc gttttctgtt
    10201 atcagccaag ggatattatg ataattttct tttagataca tggctatatc atacatgatt
    10261 ttcggataga tttcccatcc cctggaatta ttcattttct tcccaggtaa atcaaagact
    10321 tcataatatc gttcaggatg gaaaggtgtc tcttcattcc attgataacg aggggcgcga
    10381 acgcgtttag gataatattg attgattcca aggaaatcta ctgtatgatg ctgaatcgtt
    10441 tctaaatcct ctgttgcagg atcgaaataa atcgattgtt tttgacacaa ctccaggaac
    10501 tcttctgtgt agtatccttt taccatcgga tcaaaaaata ctcgattata aaacaaatca
    10561 tacatataag cagctttttg gtcttctgga gaattggatc gagcatagac catttcagga
    10621 tttaaaatac agccaatcct tccaccataa tttccgctgc ggtaaatctc tactgcttta
    10681 gccgttgcta aaactttatg ataattccac atcatccact tttttgtgtt ttgttcatgc
    10741 ggccagcgaa cagcatccaa atagcaacgc gtttggggaa cgattggttc gttgaatgta
    10801 aaccagtttt ttaccctatc accgtaacga tcaaaggcaa tttgagcata cttagcataa
    10861 aaatcaacta cttttcttga agaccagcca tcatattttt ccaaaagaaa agctgggact
    10921 tcataatgtt ccaaacaaat catcggttct acgtctgctt cgatcaaaga gtcgatcatt
    10981 tcacttatat gctgagcata ttcttcatct actatacagt tctcataatc agtaaaaaaa
    11041 cgagcccaat tgatagaagt ccgataatgt gtcagatgga tggatttcat cagatccaca
    11101 tcctgctggt attgttccat aaaattagtt gctacggctg ggccataccc attatgccaa
    11161 acaaaatgtt catttttata ccagctatct ataaacgaat cctgtccttc tttttttcct
    11221 ttccaacctt ccgtttgcca agcagaagat gcagcaccaa gaataaaatt ttttggaata
    11281 gtaattttca tgatttcctc ctgtttttta gatcccatca ttttccacac gatttgctac
    11341 ttttacaaat ggcagataga tcaaaataga aacaataata caaacaagtt gcgtaataac
    11401 tgcaccgata tttcctgctg ttgccagata agcattgacg atcggcggca ttggccaagg
    11461 gaccattaca actgctttcg ccgcaaatcc taaagcggtt gcaaaataac caatcgttcc
    11521 agtgataaga ggcgttaaga taaacggaat agctaaaatt ggattcagca tgatcggcat
    11581 accaaaaata actggttcat tgatgttgaa gatcccagga gcaagggata acttggtgat
    11641 ttgtttcata tcttcccgtt ttccaactaa caatactgca atcagtaaac cgatcgtgac
    11701 tccagacccg cccatactca tatacatatc ccaaaatggc atagtaataa tgttcggtac
    11761 ttcttttcca gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa gcaacggttc
    11821 ccgtatcggc ttcaccattt gatttccatg gataccaatc acccaaaaga cttgcgcaat
    11881 aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg ggcgttgtac
    11941 gacattgtaa atgatatcat aaaggtacat tcctgtaagt tctttgatga taaatcccgc
    12001 agccgcgata cccgatacag tcagaatcgt cgggaataaa gcagaaaaac tagcagaaac
    12061 attcgttggc acggtatcag gcattctcag ctgtagtttc ttctgtttcc tcaaccagca
    12121 ataaagttca atagaaagga tggagaccaa cattcccaaa aataaccctt tcgtatctgt
    12181 atattgttta gccaatacgt tttctactat aacagtctga ttttcattca ctaattcata
    12241 aacagttgga ttaacagata aataagacat cactgcgatg ataccaggga atacaccatc
    12301 cactttattc agcttggcta attcgatccc tatcagaaaa acagcataga tggtcaagaa
    12361 acttagtgtg acatagctga tggcagatgc gataggcttt aattcttcta aaaaacgcaa
    12421 gaaacttatc tgtgctaagc cattttccga gtcaaagacc attgctgaaa acaatgtgcc
    12481 aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat gcgtttttta tcaccatgat
    12541 ataccttaaa ttattgattc tcgttgccac gttccctaaa acattggcta atttatccat
    12601 aaaactcatt ccagatcccc tcattcctgc ttcttctcct tgacgtcatc tctttttgtc
    12661 tattagtcag cgcttacatt tcgatgaaca gaaactattt actcctcttt tttaccaaat
    12721 ataaaacaaa aattcatctg atgtatttat cataaaatac atcagatgaa ctgtcaatta
    12781 gtttcttttt ctcctattat tttctgtaat tctctccgat ttattgccat atggacagtc
    12841 attgcatcaa acgcagcaag tgcattccgc ctttcgatgg cctgaacgat ctctgtatga
    12901 atatcgatcg tctcgatttt cagatgctgg atatcataat ttttattaaa caaagcaatt
    12961 gattcattaa tgattgggat aatatgcagc aaggctacgt ttccgctagc ttctgcaatc
    13021 atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg tgccttctcg aaaagaagtt
    13081 tcaatctttt gcgttaattt tttcatttgg acgatttgat tcttcgttgc atattccgct
    13141 gccaaacctg ccatcttagg ctccaataaa tatctcagtt caaataagtc tcgtatcatc
    13201 cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc tcgcttatca
    13261 ctgacaaatg tccccgctcc ttgccgaact tcaaggatat tcctggaagc tagagcccgc
    13321 acagcttctc gcaccgtgct tcgccccacc tctaaataat cggacaattc atattcattc
    13381 ggcagtttat ctcctacttg caactgcatt tcctttaaat atctaaggat cttgtcagta
    13441 gttacttcta ctagggtttt attttcttcc aaaccacata cctccttttc atctgatgta
    13501 ttttttcgta attattataa cacgtttgaa tcatctgatg attttttgta tacaaaattg
    13561 attaatccgt tcatagacat acaaacgaag aagatattta ccataaaaaa gactagga
//
LOCUS       DAYYHX010000062        13604 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000062 DAYYHX010000000
VERSION     DAYYHX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.062"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(126..1073)
                     /locus_tag="ACHA6Y_001370"
     CDS             complement(126..1073)
                     /locus_tag="ACHA6Y_001370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM8987472.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1263..1532
                     /gene="rpsN"
                     /locus_tag="ACHA6Y_001371"
     CDS             1263..1532
                     /gene="rpsN"
                     /locus_tag="ACHA6Y_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8987473.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1708..2070)
                     /locus_tag="ACHA6Y_001372"
     CDS             complement(1708..2070)
                     /locus_tag="ACHA6Y_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8987474.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2081..3202)
                     /gene="alr"
                     /locus_tag="ACHA6Y_001373"
     CDS             complement(2081..3202)
                     /gene="alr"
                     /locus_tag="ACHA6Y_001373"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM8987475.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3218..3568)
                     /gene="acpS"
                     /locus_tag="ACHA6Y_001374"
     CDS             complement(3218..3568)
                     /gene="acpS"
                     /locus_tag="ACHA6Y_001374"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM8987476.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3698..5209)
                     /locus_tag="ACHA6Y_001375"
     CDS             complement(3698..5209)
                     /locus_tag="ACHA6Y_001375"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8987477.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5531..6883)
                     /gene="murF"
                     /locus_tag="ACHA6Y_001376"
     CDS             complement(5531..6883)
                     /gene="murF"
                     /locus_tag="ACHA6Y_001376"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM8987478.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(7036..8112)
                     /locus_tag="ACHA6Y_001377"
     CDS             complement(7036..8112)
                     /locus_tag="ACHA6Y_001377"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM8987479.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8308..10053
                     /locus_tag="ACHA6Y_001378"
     CDS             8308..10053
                     /locus_tag="ACHA6Y_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM8987480.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10333..10770)
                     /locus_tag="ACHA6Y_001379"
     CDS             complement(10333..10770)
                     /locus_tag="ACHA6Y_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM8987481.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(10754..12127)
                     /locus_tag="ACHA6Y_001380"
     CDS             complement(10754..12127)
                     /locus_tag="ACHA6Y_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987482.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12449..12937)
                     /locus_tag="ACHA6Y_001381"
     CDS             complement(12449..12937)
                     /locus_tag="ACHA6Y_001381"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8987483.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12959..13561)
                     /locus_tag="ACHA6Y_001382"
     CDS             complement(12959..13561)
                     /locus_tag="ACHA6Y_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8987484.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3896 a         2947 c         2465 g         4296 t
ORIGIN      
        1 agggaaagat aattttatgc caaagttttt atctattata cgtatacgca aaaaagctaa
       61 aacatttttt gtctttgccc ctacaaaaat gtttcagctt ttttcgtttt ttattcctgt
      121 atttcttatt tttcgaatga caggatgatt ttcccagcag gaggatgtcc ttcaactagc
      181 tgatgtgctt ggatgacatg cttaagtgaa gcagacagat tcattgaaat aaaaacatga
      241 aaggctcctt tttggtaggc tttgattatg ccagcaaagg cttctgcatc tagatattcc
      301 tttcttggaa caaacgattc ataaaagcct tccttttttt gccgcaaatc tagatctggg
      361 aggtcattta atgcaacata cctgccgcct ggtttcatga tctggatgcc ggtttctcct
      421 ttgatacttc ctttcgtcgc atcgattaca aggtctgcct gatcagcaaa ttgcagacct
      481 ggatttgttt tatcataagc agcaaatgct gaagctccta atttttttac cttttcctca
      541 tttttactgg atgcactggt taagattcgg attccctttt catggagaag ctggatcaga
      601 cttgacccga ccgcacctga tgctcctagt atcattacag tatctgtcgg ctggatttcc
      661 gtcaaatggt tgattagatt ataagccgta attcctggcg tgaccattcc ggctgcttct
      721 tcccaactca ttttatctgg aatttttgct acttttgctg atggcaacac gacttcttct
      781 ccgtaagtcc cgccgactgc atggacagct actcgatcac ctacatgaac agttgtgaca
      841 tctgaaccaa tctctgtcac aataccagcg ccatcattac ctggtacata agggaatttc
      901 aatgttcgta tctctttcat ttctcctaaa cgcaaggcaa catcataagg attcacactg
      961 aatgctttta tttctacacg aacatgtcca gaaccgactt ctctgggatg agcatcgatt
     1021 tcttcaaaaa catcttgtgc ttctccgtaa ccgttaattg caaatcttcg catggtcacc
     1081 atctcccttt ttctttaagt ttacgtttat gcctttattt tgacaaaaaa aatgtctttt
     1141 cgttttggcg catgattcaa aacaaaatag ttgtcttttt tcgcgtaacg tgttaaatta
     1201 tttatctaaa agcgtaaaga ttacgattta cgctaataaa aaataattgg aggaatttgt
     1261 tgatggctaa aaaatcaaag attgccaaag caaaaaaaca gcaagaaacg attgaacgct
     1321 atgctgcttt acgccaggaa ttaaaagcgc aaaaagatta cgaaggcttg cgtaaacttc
     1381 ctttagatgc tcgtccagaa cgattgaaaa atcgtgattt aatcgatgga cgccctcgcg
     1441 gatacatgcg taaattcggg atgtcccgta taaaattccg tgaattagca cataaaggtt
     1501 tgattccagg agtaaaaaaa gccagctggt aatccaagtc tggctgtaat gtaaaggagg
     1561 agggcgcatg gaccactcct tggatcatca aaaaagatac gttcgcttct tctagcagaa
     1621 atgctggtac tagaagcaga cgtatctttt tttacgcaca gctggtcctt ttttatatgt
     1681 aaaggctgca tggttacttc gctatattta tttagtaaca ggagtcagtg cttcgatacc
     1741 cacactaatt ttcaatgctc ggtcaacttc tgccataatg ggtcgatcca aatggcagac
     1801 cttttctttc aatcttgatt tatcgatcgt tctgatctgc tccaacagga taacggagtc
     1861 tctttctatt cctgttccag tagctttgat cccaatgtga gtcggaagct taggtttagc
     1921 catttttgcc gttatggccg caacaatgat cgtcgggcta aaatggttac ctagattatt
     1981 ttgaataacc aaaactgggc gtgttccgcc ttgctccgat cccacgactg gagaaagatc
     2041 agcaaaataa atatctcctc tttttaccat aggatcctcc ttattcttga tattctcttg
     2101 gtatccgctg tcctaatccg caagccactt catagtggat cgtacctaat tgatctgcta
     2161 tatcctgcat agtgatttct ttgtctgcat ttttgccgat aagcgtcact tttgttccga
     2221 caggaaattc ttgaggcaat cggatcatca gttgatccat acatacacgc ccaatcgttt
     2281 cacagtagtt cccttctacc aatagagaaa aaccctgcat ctttcgtggc cagccatctg
     2341 cataaccgat cggtatcgtt ccgatccact ctgcttcagg agtgatatat gtttctccat
     2401 agccgattcc ttcacctgct gggagttttt tgacttgaat caattcagaa accagctcaa
     2461 gtgctggctg caatggataa acttccggta gtgcgtgccc agagggatta agtccgtaca
     2521 ttgctactcc ataacgaatc atattgccga ttgtttcatc atgccataaa gccgttgcac
     2581 tattgctgac atgtacatat cgaggaagtt cgggaagttt tttcagcact tctataaagc
     2641 gttctttttg cagattccaa tatgtatcat ctgcctgatc tgctgtagaa aaatgggtaa
     2701 ataaaccttc ccattctaat acacgacttt ctttgatgaa tgcagctgcc tcttttactt
     2761 cttcaggtgt acaaaatccg atacgaccca tcccagtgtc tgcttttaga tgaatcgaaa
     2821 gaggcgtctc tagcttactt ttctctaaaa cagcagctgc tgcttgcaac cattcccgtg
     2881 tacctgcagt tactgataaa tcatgagcaa cagctacagt cagtgcgtcg taaggaacca
     2941 tattcaagat aaggatgggc tgagtaaatc cagcttctct caactcgact ccttcatcta
     3001 aatttgaaac acaaaagccc gaagctccac ctgcaactgc tgcctcagct gtttcaatag
     3061 ctccatgacc gtatccgttc gcttttacta ctgcaaacaa ttcttttcct tgcggaagtc
     3121 ttgcaacttc atttgctaca ttttcagtta tagctttttt atgtatcact gctttcgttg
     3181 ggcggtgcca agcgacaacc atcgtttttc cttctttcta gtcttccaaa ataatttgtg
     3241 caaaagctgt ttcatttgta tgagtaatac tgacccatac attgccttca tgaggtgaac
     3301 gcgtcacaac aggctgacca ttttcatttt ttaaaatctc gacatcttga aaggtaactt
     3361 ttccgatccc agttccccaa gctttagaga aagcctcttt gcaagcgtaa cgcccgccta
     3421 aaaattctac ttggcgatga aaaggcaagg actggaatag tttcatttca tcagaggtca
     3481 agattcgtgc aatgaatttt ggtttttctt cgatcagccg agtgatccta ggaagttcga
     3541 ctgcatcgat cccaattcct tttatcatat gcttttctct tttcaataaa tttagaacac
     3601 tctcattcta ccaaattata accattttgc cacaaataag tccacaaata acaaaaatga
     3661 aagtttctct taaaaaagag aaactttcat tttatgttta atcgttattt gaacggatta
     3721 caaatccgcg ttttttatca ttgtgacgtt taccgccatc tttttggctg ttattatttt
     3781 tgctataacc gccttttgat ttgtttccac gataattacc gccgtcacga cgattacggt
     3841 tgtttccgcc accacgtttg ccatttttat tgtatccttt tttctgcatt ggcaatgggc
     3901 gttctggtgt gatcttcact ggcactgcat cagctggatc ttttgctgtc gtttttagca
     3961 aaagtgcaac taaatcttgt gcagaatatt cttctaataa tttatctgct gtttgcaggt
     4021 atttatctag accattttct gcaagttttg tttcgatttg ttcaactgca gcacctaatt
     4081 gacctttgaa tgcttctttt tcggttggtg gacgtaaagt cgtcatccgt tttttcgtca
     4141 agttttcaat cacatgcaag tagtccattt catttggtgt aacgaaagtc acagacatac
     4201 cgccttttcc ggcacggcct gtacgtccga tacggtgaac gtagctttct ggatcttgag
     4261 ggatatcata gttgtacaca tgagtgacac ctgaaatatc taatccacgt gcagcaacgt
     4321 ctgtcgctac aaggatatct aaatgaccgc tcttaaatga acgcaatacg ctcattcgtt
     4381 tttgttgaga caaatcgcca tggattcctt ctgcacggta accgcgtgct tctaatccgc
     4441 gtgccaattc gtctacacgg cgttttgtac gtccgaagac gattgttaat tcaggtgttt
     4501 gaacatcaaa caagcgagtc atgatatcga atttttcgta ttcttttgcg cggacatagt
     4561 attgatcgat caagtcagct gtcatttctt tcgctttgat cttcacatga tgtggatttt
     4621 tcatgaattt aacgccaata ttcttgatag ctggcggcat cgttgctgag aaaagtaatg
     4681 tttgtctttg atcaggaact tgtgaaatga ttttttcgat atcttccaag aagcccatat
     4741 tcaacatttc atctgcttca tccaaaacta atgtttgaac ggttccaagt ttcaatgtgt
     4801 ggcggttaat gtggtcaagc atacgtcctg gtgtacccac aacgatatgt ggacgatctt
     4861 tcaagccacg gatctgacgg ccaatatctg ctccgccgta aacggcttgt acacgtactt
     4921 ttttgtcgcg ccctaaacga tataattctt cttgtgtttg gatagctaat tcacgtgttg
     4981 gtgcgataac taaaccttgt aattcgtgac gatctggatc gattttttct aacattggta
     5041 atccaaatgc agctgttttc cctgtacctg tctgtgcttg tccaataacg tcttttccag
     5101 ctaatgctag tggaatcgtt tctgcttgga taggcgttgc ttcttcaaaa ccagcgcgtt
     5161 cgactgattt caacaattct ggtgataatt ctagttcttt aaatttcaaa tgaatcctcc
     5221 tatatggttg ttaactgagg atgtagcttt tttgctaccc cgtcgatttg ttcggtcaaa
     5281 gtgtattgtt tctttttgac agtggccacg cacgttttta tccattctta gcccctgtgg
     5341 caccaccgaa aaatcacttt gcgaagttta ggcattagaa aagccaaacg ggtcgcgttc
     5401 agctttatca ttattatgac atctggcttc aacaagttgt ttctatggca aaaccagtcc
     5461 aatttctctt catctttcgg caataggtta ttctaacata gtttttcgtt ttctgcaagt
     5521 ttacagattt ttatttcatt tgctgaagtt gttctaccac ttcaaataag cccatgctgt
     5581 tgctgccttt tagcataata ctatctgttg cttcaagatt ttgtttgata gcatcaatca
     5641 atttattctt ttctttttcc aaatagaaca caggtagttg ggggtatttt tggttcaatt
     5701 cttcttttag tgcatgcatt tgttccccat aaaggaaaat catggcgaat ttctcaggat
     5761 cgatatgttc actcatttgc gcatgcatat ttaaagactc tttccctaat tccagcatat
     5821 ctgctaacac agcgattctg cgtccttttg ttggcagatt tttaaaagta tctaatacca
     5881 gtcccatggc tgttggattt gcattgtaga catcgctcaa aatagctgca ccatttcctg
     5941 ccgttagcca ttcggttctg ttttgagtga tagaaacatg ggcgagccct tggcgtatct
     6001 caggtatgga cagtccaaag aagcgcccga tcgtatacgc aatcaaagca ttcgttacat
     6061 tataacttcc aggcagcggg atcgtaaatg tttctccttc tactacaaaa tcggtttgtg
     6121 cttttccttt tgctgtcact tccgcatgaa catcgccatt tccaataccg aatgttgtga
     6181 ctgtttgtac taatgttgcc gttaaaggtg tcagcaacgg ttcatctgcg ggcacaacga
     6241 gtaatccgtc tgatacaagt ccgtcagtga tttccatttt ggctttagcg attccttctc
     6301 gcgacccaag attttctatg tgtgcttcac cgatcattgt gattgctgca gcttttggtt
     6361 gagccataag tgaaagttcc gtgatttcat tggcgtgatc catgcccatt tccaaaacga
     6421 tcttttctgt atcttggggc atgtgcaaaa tcgtgtaagg aagaccaata ttattgttgt
     6481 aatttccttg cgtcttatat gttttgtatt tttcagacaa gacagacgcg gttagatctt
     6541 tcgtggtggt tttaccattg cttcctgtaa ttgcaacgac ttctgcgcct actttttcca
     6601 agtaataggt tgccaattgc tgcatggctt tcaaaggatc tgctacagga ataactggaa
     6661 aattttttgg agcttgttca acaggcaagc tccaaaatga agcagatgct ccgtttgtga
     6721 tcgcttgctg gataaattca tgtccatctc gcgtaccagc aagaggaacg aataaacttc
     6781 cttcggttat cttccggctg tcaaattcga cattggtaat ctttgtcagt gcagagcatt
     6841 cgctgcccac ggcttgtgca atttcttgta ttgttagatt cattgagatc tctcctaaaa
     6901 aaggttgtga aaaaagcgtt tggcctaaac ggcgggttcg catgactctc acttccaagt
     6961 aaatttctct attcgtattt agaagctaga atgaacgcgg ttacgcaatc actccagcct
     7021 cttctttatt tctctttatt catttttttc aaaaaaagat tgacgctgat ggtatcgatt
     7081 cattcctaac tggatcagtt cttcaatcaa atcaccgtat ttcaagccca tattttccca
     7141 taagagtggg tacatactga actccgtaaa tcctggcata gagtttaatt cattcaggaa
     7201 taattcattt ttatttgtca aaaagaaatc gcaccggctc aatccgcttc cacctaacat
     7261 cgtgtaagct aacttcgcgt actcttgcgc tttttgataa acttcttctg gcacttcggc
     7321 tggaatctgc atttcgattt tattattgat atattttgct tcataatcat agaatgctac
     7381 gtcttttacg acttcaccag gcaaagtcgt ccgaacgtct tcatttccta atacagcaac
     7441 ttcgatttcg cgcgcttcaa ttccttgttc aacgattgct cgagaatcat actgataggc
     7501 tgttgctaaa gcattttgca gctcttctcg gttttctgct tttgtaatgc cgacactaga
     7561 acccatattc gccggtttga caaacatcgg ataaagcaaa gaaccttcac attgatcaaa
     7621 tactttttta ggattttctt tccattgatt cttaagtact ggtacataag gaacttgcgg
     7681 cacaccagca gcttgtaaaa tatacttggt catgatttta tccatggcac atgcactggt
     7741 caatacgcct gcgccgacat aaggcatatt caatgtctct aagaagcctt ggatcgttcc
     7801 atcttccccg tttggcccat gtaaaactgg aaaaacgatg gctccttctt ctttgatttc
     7861 gcccggattg atcacttttc ctgtaaagcc ttcctctgtc tgtccacttg ggtcccatga
     7921 aagatgcaag acatctttgc tggcaggttt ttctgttagt aatggacctt tgacccattg
     7981 tccttctttt gtaataaata cgagttgaac ttggtaataa ttataataaa tggcatttaa
     8041 aactgaaaat gcggaaagaa tggacacttc atgctctgcg ctgcgtccgc catatagtaa
     8101 agtaatcttc aaaagattgt cctcctgtat tctaaatcgt tcagtgattt actctcatat
     8161 tttagcacaa aacaaaaata aacgaataac ttatttataa aaaaacgagc cagcttctaa
     8221 aagaacgagc gtcatttatt ttttgtcagc tagattattt gtcccgtttt ttataaaaag
     8281 ttatgataaa gaaaaggagg gatgatgatg gcgcaaacaa atccacgcag ccgcttggca
     8341 gaaatcattc gagtgttcat caattacaat gtagtgccaa actttgtaca gcaaaaaaat
     8401 cccgaacagg taaaaaaagc ctttgaagaa ctaggcccca ccttcatcaa gataggacag
     8461 atgctttctg ttcgagaaga tttgctttct tctgccttca ctcaaacatt caaaacatta
     8521 caagatagtg tacctagcga cacattttca acggtcaaaa agacgatcga aacagaactt
     8581 tctctttcat tatcagatat attcgatgat ttttcaaaat cgccttttgc ctctgcttct
     8641 atggggcagg ctcatcgtgc caagctgaag aacggtgacg cggtagtcgt taagatccaa
     8701 caccctaaca tcgccgaaga gatccgactt gatcttcaac tatttgaacg agcgatccct
     8761 ttgatcaaat acattcctga aactagcgta gttgatctaa aaggggtact ccaagaagta
     8821 aaacgttcct tgatcaatga aatggacttc ttaaaagaaa gtcaaaatgg cgaacaattc
     8881 tatcaaaaga acaatggctg gaaagagatc cgcagcccca agatttatga cgctttttgt
     8941 tcgaaaaaag tgatcgtgat ggaagaaatg tctggtaaaa acttgaatca tttaatgaac
     9001 gccgaaaaca aaactgaaac ttttatcact ggaattcaaa acaagcagct gaaacaagaa
     9061 gttgcaaagc ttttagttga gaactttatg aaacaggtgt ttgatgatgg ttttttccat
     9121 gctgatcctc atccagggaa tctgctattc catgtactaa caaaagaaga acagacacaa
     9181 gctagccgaa aaactgaaac agtccatgaa aaagagttcg gttcctttgc ttttcgagcc
     9241 tccacttctg cagaagaccc tgttgcgcca tataccatca actacatcga ttttggtatg
     9301 atggggcatc tttctgcagg acttaggcaa aaactcacac aagccgtttt agcgctgtat
     9361 acgaaagatg cttatcgaat cgaaaaagcg gtccttcgac tttgtcagca agaaggctcg
     9421 tttgatgaaa gtcggttcca ccaagaactg acaagttttc ttgaacagta ttatgattct
     9481 cctattgatg aaatcaactt gcaagaagta ttcacacatg tcgtgacgat ttgtcatcaa
     9541 aataatctgc agttcgatcg tgatatcacg cttttgctga aagcatttgg gacattagaa
     9601 ggggtgatcc gcgtcctcga tcctgaagtt tccctcatgg aagtcgcttc gccttttgct
     9661 caacattatt ttctcactca tctggatgta gaagatacat tgaaacaaag cggattagac
     9721 ttattggaag ggatgaaggc tgcgcctaag atccctcaac agctccacca tctattagaa
     9781 atgtggactt cgggacaagg aaaagtcaat ttggaattga aaaagcaaga caaattattg
     9841 agcaggatcg aaagtatgat caatcggctt gttttcggta tgattttagc tgcattgatc
     9901 gtagggtcct ccctgcttgt ccaagcagca cctgtagaaa acgcagaggt cgtttcctta
     9961 ttagggatat ttacgtatgc tattgccgca ttcgtcatta tttttttggc aatcgatgca
    10021 ttgatccaaa tgtataagaa gaggaaaaag tagaaaaagg ttgtgacaga aagaatcagc
    10081 ttcaagaaat aagaaggggt tttacataaa ttgtttctca aatttttgtg aaaattcggc
    10141 ttatttttcg aagaggctac ttctgaaaca ccgagaataa aaaatgtgaa aaagctgttc
    10201 tgcacccagt aataagaaca atcaaaaagg gaacatggaa cacatttatc ctgtttccct
    10261 gttccctttt tgatgtattc gagcataaat aaaatacctt cgttacctac tatcttctgt
    10321 acttggtaaa ccttagtcaa ttgccttttt taacgcttga cgaaaagaca tatgatcttt
    10381 ctccgtaaag ggttttggac cctttgtcct tatgccagct tttcttaact ttccactatg
    10441 ataacgttgt tctagaagcg tgccgatatt cgcttcgtca aattcttttc cagagtgatg
    10501 gaacactcgc acacccttcg gcaatacaag tgaagctaaa ccataatctt gcgtgatcaa
    10561 aaagtcattc ttcttaacca taccgacgat tttataatcc gcactatcag ctccgcggtc
    10621 tacgtagacg atttggacat gattagcgta ttctttggtc gtataatggt ccacactggt
    10681 cacgataacc acgtcaagac cataggattc tcctaatttt atgacatctt cttttactgg
    10741 agaaccatct ccatcaatga atagcctcat tctttttcgc cttcttccat aaatgataaa
    10801 cagttactaa tccgctcagt aaaagaccaa cattgatcca ttttgccaag ataagcagtt
    10861 gtacactttc tatcggcact gattcatggt tcagcgtgat caataataga tggattggct
    10921 ctcccatttc ttttgctaaa acaaacaaaa gcgtcatcaa taaaaactgg tggatcacac
    10981 tccctttatt ttgagtagta tacttgacaa cgatccggcg atccattgga atagctgtat
    11041 cagtttgata atattcctta tttagccagc tgcctgttac tacgtgccag aaactaagca
    11101 gaaagacacc tatcgcaaaa ccatttggaa tcaagaggac caataaagaa gaactcagtc
    11161 caagcaattg catagccaat ggagaccatg ttaaacgttt caaaatccat ggtccgacta
    11221 tttttgctgc aatcaagcct gctgcataag gcaaatacat aaaaatcgaa agtttttcta
    11281 atccgtacag tatgccaaca taaaaactgc taaataaaaa aaggaaattc cctagcattc
    11341 catttagaaa agtaaacaga tttagccagc ttggcaactt ccattccttc ttctggtgtc
    11401 ctaaaacaaa cacgaacaag atgaacagca gcaagaaacc ataaatagca agatcaaaaa
    11461 gcctttcatc gaataacaat cgggcatttc tcaaaagaaa caataagcca aagaatatca
    11521 aaaataaaac aagctctcgc gtagctacca gatttctttt gatgtctttg aaatccagtt
    11581 cgtaacgcgg ataatgacta accgtatggt aggccatgac gtaaaaaagt gtataaatag
    11641 taaagcttgc tgcaatctga gtcggtgcag gcaactcaat cgcttgatac aggagaaata
    11701 gccagatagc tgcaaataag tactttttcc catccatatt gataaaacct tgcgtctttt
    11761 cgtgaaaatt gaccgtggta ttagcaggta caagccatgc tgcactcagc cctagcaaaa
    11821 cacttgaaaa ataaaataaa ggaaaataaa atacgcctaa tattccaagc aatgaaccag
    11881 caccaccaat cagcagagcg atcatcagca acgtgtaact atctagacca aatcgtagac
    11941 tgttgactaa aaatagtcca gtcatccgaa aggcataaaa cagcgtaaat ggcaaaatcg
    12001 tcagccaatt atgcgagtct gttagtccca aaaataaaag ataaggaata aaaataaccg
    12061 tgttacttaa gataaagggc gttgtttgtg taaaattacg cccaaatcgt ttgagttttt
    12121 cgaacatgac aatggctcct taaacagtct tataactaat ctgctcttcg attttagcat
    12181 taaaggggaa ataaaccaat tttacagaat atattcttgt aaaaaaaaat acagaaagaa
    12241 gcataagctt ctttctgcat ttttacggta cttgattact caagcagaca agcccttctc
    12301 cacaaaagtc ttgcccgaca cccctgcatc cggcaggtga taaagataga aatcaattat
    12361 cctgtgcctc caaaatcaat taggagctct gtaaaccaga gtccttctcg acccgtcgca
    12421 taaatttact ttaacacagt tcctatgttt atgcgagagc ttttttcatt ttataatcga
    12481 ccatttgaaa tccttgaccg atatctgaaa taagttcttc tgtaatctca aatccttcat
    12541 gcttataaac ctcgattgct tgatgattcc cctgattcac tcgcaaaaag atctctcttt
    12601 cctttgtttg cgcagcaagt tcttcatacc agcgaaaaac tgaagaagtc aatccttgac
    12661 cacgtacttt atttagcaaa tataatttac ttaaataaat ttttccattt atctcttcat
    12721 aagcagtgta accgacagtc ttttcttcgc tttttaataa aaagtaggac acaccttctg
    12781 tcagttcttt ttgaatctgt ttttttgact gataagtttt cagcatgtaa tccacttgtt
    12841 tttgaccaat gatcggcaca aaagcttccg gccatacttc ttgtatgact gccatcagct
    12901 catccagttc attttctgtt acttttattt tttccataca cccacgtcct tttttacatt
    12961 ataaatgacc ttgcgcttga tggaataaaa agagcatgat catgctatac acacccaccg
    13021 accatggttt caagaaaatc acaatctgca taaattccct aaaagataat ttcgtcaaac
    13081 cagctactag acagatcaag tcgtctggcg caaagggaag cagcaaggta acaactagta
    13141 cttttttaat atctttcgtt tttcttgtca gcaattcatc aaatttctga tacttttttg
    13201 gcgataaaat aagctggaca aaggctacgc catagtaacg gacaagcaaa aatccgattg
    13261 cttccccaat cactaggcca atacaactat ataaaagccc aaatccattg ccaaacatca
    13321 gcatgcccgc tactgaggag atccctcccg gcaaaatagg aatgatcact tggatcactt
    13381 gcaacagaac aaatatcatc actgcatact tgccaaattg ctggataaac gcttgcaaag
    13441 acgtgctcga tcgaaaaatc ccatgatgat aagcataaag aatcaacaca aagaaaaaaa
    13501 acagaccaat aatgggcatg atctgaagac atttttgtaa aactgatcct tttttagaca
    13561 tctatattcc ccacatcttt tttcatttat taaaactgcc tcct
//
LOCUS       DAYYHX010000063        13359 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000063 DAYYHX010000000
VERSION     DAYYHX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.063"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            333..947
                     /gene="gmk"
                     /locus_tag="ACHA6Y_001383"
     CDS             333..947
                     /gene="gmk"
                     /locus_tag="ACHA6Y_001383"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8987485.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            952..1257
                     /gene="rpoZ"
                     /locus_tag="ACHA6Y_001384"
     CDS             952..1257
                     /gene="rpoZ"
                     /locus_tag="ACHA6Y_001384"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8987486.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1510..3918
                     /gene="priA"
                     /locus_tag="ACHA6Y_001385"
     CDS             1510..3918
                     /gene="priA"
                     /locus_tag="ACHA6Y_001385"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM8987487.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3933..4424
                     /gene="def"
                     /locus_tag="ACHA6Y_001386"
     CDS             3933..4424
                     /gene="def"
                     /locus_tag="ACHA6Y_001386"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8987488.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4417..5355
                     /gene="fmt"
                     /locus_tag="ACHA6Y_001387"
     CDS             4417..5355
                     /gene="fmt"
                     /locus_tag="ACHA6Y_001387"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM8987489.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5355..6713
                     /gene="rsmB"
                     /locus_tag="ACHA6Y_001388"
     CDS             5355..6713
                     /gene="rsmB"
                     /locus_tag="ACHA6Y_001388"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM8987490.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6726..7466
                     /locus_tag="ACHA6Y_001389"
     CDS             6726..7466
                     /locus_tag="ACHA6Y_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM8987491.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7463..9538
                     /gene="pknB"
                     /locus_tag="ACHA6Y_001390"
     CDS             7463..9538
                     /gene="pknB"
                     /locus_tag="ACHA6Y_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM8987492.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYNYSDTVPSGSIISQDPASGEVVAEET
                     YINFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVP
                     GAGTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSS
                     SASTSESSK"
     gene            9703..10602
                     /gene="rsgA"
                     /locus_tag="ACHA6Y_001391"
     CDS             9703..10602
                     /gene="rsgA"
                     /locus_tag="ACHA6Y_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM8987493.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10615..11265
                     /gene="rpe"
                     /locus_tag="ACHA6Y_001392"
     CDS             10615..11265
                     /gene="rpe"
                     /locus_tag="ACHA6Y_001392"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8987494.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11266..11904
                     /locus_tag="ACHA6Y_001393"
     CDS             11266..11904
                     /locus_tag="ACHA6Y_001393"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM8987495.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12059..12913
                     /gene="mreC"
                     /locus_tag="ACHA6Y_001394"
     CDS             12059..12913
                     /gene="mreC"
                     /locus_tag="ACHA6Y_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM8987496.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12917..>13359
                     /gene="mreD"
                     /locus_tag="ACHA6Y_001395"
     CDS             12917..>13359
                     /gene="mreD"
                     /locus_tag="ACHA6Y_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM8987497.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTL"
BASE COUNT         4418 a         2473 c         2957 g         3511 t
ORIGIN      
        1 gtattaagcc aaatttatgc aaaaatgtga gaaactattt tcacaaaatt tttcttcata
       61 ctcgaaacta atcggcttct tcacgacctc ttatacacgg cgttcaaaag cttgcatctg
      121 cgataataac cccaaccaaa cgaaaaacat gagaagctgt ttatgtttgg ctgttcttat
      181 tacttgatgc aggacagctt ttttacgacc tcttggtaca gggtagcttt tttaccaatt
      241 tctagctata attaaagata accttgaaag ttgcagaaaa aaagtgcaaa ataaacgata
      301 agtacaaaaa gactagttga aaggacgtcg tcatgtcaga gcgtggatta ttaatcgtgt
      361 tgtctggtcc atccggtgta ggaaaaggca cagtacgaaa agcaattttt gagagtgagg
      421 ataatgattt tcagtattcg atctcaatga ctacccgtaa aatgcgtgaa ggggaggtcg
      481 aaggcgttga ttatttcttc cgaagcaaag aagagtttga atcaatgatc gaagctggag
      541 agatgctgga atacgcagaa tatgtcggga attattatgg aactcctttg tcatatatca
      601 acaagacatt ggatgaagga aaagatgttt tcttggagat tgaagtccaa ggagcaaaac
      661 aagtcaaaga aaaagtgccc gatggtgtat tcatcttttt gactccgcca gatttagcgg
      721 aattaaaatc acggatcgta ggccgaggaa cagatgcaga tgaagtaatc gaagaacgaa
      781 tgaaagttgc aaaagaagaa atcgaaatga tggctcttta cgactatgct gttgtaaatg
      841 atgaagtgcc gctagcagtt gaacgaatca aaaacattat tgccagtgaa cattttcgag
      901 tagatcgcgt aatcggtaaa tatattaaaa tgttaaagga gatgtagtct tatgatgtta
      961 aaaccttcaa ttgactcact attagatcgt gtaaattcaa aatattctct agtgatttta
     1021 gcaagtaaac gcgcgcatga attggatgca agtgcacagc ctactctaga ttcttttgat
     1081 tcagtaaaaa gcgtcggtca agctttagaa gaaatcgatg caggaaatgt agtcaatgat
     1141 ccacatcctg aattaaaacg tgaacgtttg aagatggaag aagaggaacg tcaagcacaa
     1201 aaagagcgtg aacaacacga attggaatca cgtatccgtg aagagcaaaa aatgtaataa
     1261 agaaaaatag agcgaacatc taaaaagaaa agcaaaccca gctgtttcct tttcaaacat
     1321 tcgccactta ttgtctggta gaaaaggctg tgtctgaact ttttgacaca gttctttttt
     1381 ttcttatctg ctgttaattt tttgttagga tttggcagaa gataagacaa atccatcgat
     1441 ctctagtaaa ataggcaagg aactttttgt cgcttgataa gaaaagacaa aaaaaggagg
     1501 aaacgaacca tgacattaac agctgaagtc attgtggacg tacctactat gcagaccgat
     1561 cagccattca cttatttggt tccctctgaa atggaaacag ctttgcaagt cgggatgcga
     1621 gtagaagtcc cttttggtaa tggcaatcgt catgtccaag gatttgttat ggctatccaa
     1681 aaatcagaag aatcagctaa tccatctttg aaggcagtta ttcgtttgct agaccttgct
     1741 ccggtggtca atgaagaatt actttcatta gctgattata tgaaaaaaat tacttacgca
     1801 ttcaaaatca cttgtctaca aacgatgctt ccgagtgtga tgaaagcaga atatgataaa
     1861 ttgatctatc ctttggcaga cacaccagaa gttcaggtat tgtttgatca gcgagaagtg
     1921 atttcctgga aggaagcaga agaagaaggc agcctttctc aattgattcg ctggcgccaa
     1981 gagcaattag tagatatcaa atatgaagtc catacacgaa ataaagtaaa aacgattcgt
     2041 ttggtccgtt ctttgttaac tgaaaagcaa atcgaagaag aatgggcaaa attacggcaa
     2101 aatgccaaga aacaaaaaga acttcttttg tgtttatctg aactgagcca agaagaacca
     2161 attgcttatt tcaaaaataa aggaatcagt actgctgtct tgaatcaagg aaaagaaaag
     2221 ggctggctgg agtttataga atcagaacgt tatcgcgacc cttataaaga ccgagtattt
     2281 gaccaaacga cggcattgga actgaatgca gaacaaaaaa atgctgtcga acaaatcatc
     2341 acagcaggtc agcagcaaaa agatcaggtc tttttgctgg agggaatcac tggcagcggg
     2401 aaaacagaag tatatcttca ggcaatcgct gatgttttgt cagaaaataa aacggctatt
     2461 atgttagtgc ctgaaatcgc attgactccg caaatggtcg agcgattcaa aggacgcttc
     2521 ggagaatcag ttgcggtctt acatagcgga ctttcccaag gtgaaaaata tgatgaatgg
     2581 cgcaagattg agcgggaaga agcgcaagta gtagtaggcg cacgatctgc tatttttgcc
     2641 ccgctgaaaa atatcgggct gatcattatc gatgaagaac atgaatcaag ttacaaacaa
     2701 gatgagacac cgcgttatca tgcgagagat ctagcaattt ggcgcagtaa atatcatcat
     2761 tgcccaatag ttctaggaag cgcgacgccc tctttagaat caagagcacg agctcaaaaa
     2821 ggcgtttatc agttattgca gttaaaccac cgagccaaag cagctgcaca gcttccagct
     2881 attgagatcg tagatatgcg tgaagagttt cagaatcatc ggacctctac tttttctgct
     2941 aatttgcagg aaaaaataca aaatcgatta gacaaaaaag aacaaactgt cctactgctc
     3001 aatcgtcgag gatattcgtc ttttgtgatg tgtagggact gtggatttgt tcttccatgt
     3061 cctaattgcg atatctctct tacactgcat atggatacac gttcaatgcg ctgccattat
     3121 tgcggacatg aagaacctat tcccaatcgc tgtcctaatt gtggcggcaa taaaatccgc
     3181 tactatggaa caggtactca aaaagtagaa gaggaactcc gagaacttta tccgcaagct
     3241 aggattttgc gtatggatgt agataccact cgaagaaaag gggcacacga acagatttta
     3301 caaaaattcg gtgcaaaaga agcagatata ttattaggga cacaaatgat tgctaaaggt
     3361 ttggactttc cagaagtgac attagttggg gtgttgaatg ctgatacctc cctgaattta
     3421 ccggattttc gttctagtga gcatacattc caattgctta cccaagtttc aggacgagca
     3481 gggcgagcgg aaaaagcagg cgaagtcgtg atccaaacat tcaatcctca acattacgcc
     3541 atagaattag ctaaaaaaca aaattatgag cagttttatc agcaggaaat gcatgtacgg
     3601 catcgtggag gctatcctcc ttattacttt actgtgaaaa taaccgccag ccatcctgaa
     3661 gaacaagtag cggcaaagaa aatctttcag attgccaatc agctaaaaga agtgttaagc
     3721 cctcaaagct tgctattagg gccaacgccc agcatgattt tgagagtgaa aaaccgatat
     3781 tattatcaac taatcatcaa atataaacat gaaccgaatc ttcctcatgt tttggatgaa
     3841 attttgaatg gaagccagaa agaacagcga caaggtcttt ttgtggcaat tgacaacgaa
     3901 ccattaaact ttatttaaga aagggccaaa ttatgcgcta tcctatattg atccacccta
     3961 atgataaatt aaaaagaact gctcagccaa tcgatgtaat cacggacgag acaatcgctt
     4021 tattagacaa tttatatgag acaatgattg cgaatgacgg aatagggatc gctgctcctc
     4081 aagtaggaca aaacaagaga attgctgtga tcgaagtaga tgaaggcgag aaatttgaac
     4141 tgattaaccc agaaatcatc gaagcaaaag gtgaaagcct tgacgtagaa ggttgtctca
     4201 gtattccgca tgtctatgga acggtcaagc gagcagatga agtgaccgtg cgttattatg
     4261 atcgagacgg agaagagata gaagtgacgg cttttggcta tttagcccgt gccttccagc
     4321 atgaaatcga tcacttagac ggtatcttgt tcattgagaa aatgatacag cagatcccgg
     4381 aagaagaact tgaagaatat atggaggaac atcttgatga ctaagctagt atttatgggc
     4441 acacctgctt tttctgtgcc gattcttgaa gggctgatag aatcaggtta cgagatccag
     4501 gcagtggtaa cacagcccga ccgtccagtt gggcggaaac gagtaattac gccgacaccg
     4561 gtaaaagaag cggctctgaa gcacggtatc cgtgtgctgc agccagagaa aatctccggt
     4621 tcgccagaaa tggaagaaat cattgaattg gtgcctgatg tgatcgtcac tgcggcattt
     4681 ggccaattct tgcctgaaaa gttattgcaa gtaccaaaat taggggcaat caatgtccat
     4741 gcttctttac ttcctaaata tcggggaggt gcgcctgttc attatgccat catgaatggg
     4801 gagaaagaaa ctggtgtaac gatcatggaa atgatcaaaa aaatggatgc aggcggaatc
     4861 tatgcgcaag aaagcatacc aatcactaaa caagatgatg tagggacgat gtttgaaaag
     4921 cttagccttt taggaagaaa gctattgcta gaaacattgc ccaatatttt ggatggacaa
     4981 aaaccagtac ctcaagacga gtcagaagca actttttcgc caaacatcac acgggaacaa
     5041 gaagcaatcg attggcataa aactgcagaa gaaattgaca accaagtacg cggaatgcgt
     5101 ccttggccaa ttgcattcac gacctatgag ggcatacgct ggaaattatt atcggttgaa
     5161 gtattggcag aaactacaga caaagcacct ggtacgatca tccggaaaga caaaaaaaat
     5221 ctatggattg cttgcggtaa acagaccgtc ttagccatca atgaattaca gccagctggt
     5281 aaaggaaaac aggccgtaca tgatttcctt aacggaagcg gtcagcaagt tcagattggt
     5341 cagcaggtga aataatgaaa aaaatcccta aaaaaatgaa aaaatcagta cgatatatgg
     5401 cattactgac attagagcga gtgaacaagg gtggcgccta ctcgaatctt ttgttgaatg
     5461 agatgatcaa acaaagcgag ctaaatcaaa aagatgtggg tttatttaca gaattgatct
     5521 atggtacgat tagccatcag cgactattgg aattttacgt tgcaccattg atcgctaaag
     5581 caaaaaaagt ggatgactgg gtcaagacgc ttctttatct ttctgtatac caattagaat
     5641 ttttagataa agtccctgca catgctattt taaacgaggc ggtcgaaatc gcaaaagtaa
     5701 aaggaaatcc tgggacaggg aaatttgtaa atggtgtctt gaggaattat cagcgtcaag
     5761 gagcaccaga tctgcaaggc attgctgatc ctatcgaaag actttctgtc aaaatcagcc
     5821 tgcctctatg gatgacgaaa cgactggtgg atcaaatcgg atacgaagaa actgaaaagc
     5881 tcggtttatc gctatatcag cctagccatg ctagtgcccg agtagataca cgccgacttt
     5941 ccagagatca ggccatcact gttttgcaag aagaagagat cacagctgaa aaaagccaag
     6001 tcagtccata cggtatagta gcgaaaaaag ggcatttagc ttctagctca ttgttccacg
     6061 atggcgtcat gacgatccaa gatgaaagtt cgatgcttgt agcgccgtct atgcaaatcg
     6121 agcctgaaca tcatgtctta gatgcctgtg cagcacccgg aggtaagacg acgcatattg
     6181 cttctttttt agatccgtca tgcggtggag aagtggtcgc tttagatgta cacgaacaca
     6241 aagtcaaact cattgaagat aatgcagcac gtctaggtgt ctcttctgct gtctacgctc
     6301 aaaagatgga tgctagagaa gtcagtaaac agttcgaagc agagcagttt gatcggattt
     6361 tagtagacgc gccttgttca ggtcttgggc tgcttcggcg aaaaccagat attcgttata
     6421 aaaaacagcc ggatgaatta gcgaatttac caaaaatcca attggaaatt ttagaaagct
     6481 gcgcaccaac tttaaagtct tccggtattc tgacttatag cacttgtacg atcttaaaag
     6541 aagaaaacca agacgttgtt tctgctttct tggaaagaca ccctgatttt gagaggattg
     6601 atgttttgac caatcctatg ctacaatcat caatcgaaga gaaaatgttg actttgtatc
     6661 cgcatcagtt ttacacagat ggatttttca tttgttgttt gcgcaaaaag taatgaggtg
     6721 aaaaaatgca gattgaatat caatcagatg taggaagacg acgtaacaca aatcaagact
     6781 atgccagtgt atttaccagc cgttcaggtc tcaagcttgc tgtattagca gacgggatgg
     6841 gtggtcatcg tgccggagac attgccagtc agatggcggt aacgaatcta ggcgaagctt
     6901 gggaaaaaca agaagctgtt gacgatgaaa aaatcgccca atggttcatt caatcgattc
     6961 aagaagaaaa tatgctgatt tatcagcgtg gacaagaaca gccggaatat aacggtatgg
     7021 gaacaacaat cgtagcggcc gctctgtcag atgactggtt cacgattgcc aatgttgggg
     7081 atagcagagc ttatttgatt cgtgaagata aaatcatgca attgacggag gatcattctt
     7141 tagtcaatga actagtcaaa tcaggagaaa tcagcgaaga aatggcagtg aaccatccaa
     7201 ggaaaaatat tttaacacgt tctgtcggca tgcctggaac agtcgaagta gatgtttcta
     7261 ctcatctttg gcaactaaaa gatcgactgc tgttatgttc agatggcttg acgaacatgc
     7321 tgtctgaaga agaaatagga acgatcatca atcaagaagg cccattgtct cataaagtaa
     7381 ctgagctgat tgatcgtgcc aatgaagcag gcggagcgga taatatcacc gttcttctta
     7441 tcgaatataa ggaggacagt gtatgatcga gttgggcaaa aaattaaatg gccgttatca
     7501 aatcactgga aatatcggta gcggtggtat ggcaaatgta tttttggcgc acgatttaat
     7561 cttggatcgt gatgtagcag ttaaagtctt acgatttgac tttcaaaatg accagactgc
     7621 gattcgacga tttcagagag aagctttagc agcaacagaa ctcgttcatc caaacattgt
     7681 tagtgtttac gatgttggtg aagaagacaa catgcagtat ctggtgatgg aatacgtcaa
     7741 aggaatggac ttgaaacgat atatccaaac acattatcct gttccttatg aaacagcagt
     7801 aaatattatg cagcagatcc tttctgctat ttctttagct cataaccatc aaatcatcca
     7861 ccgagatttg aaaccccaaa acgtactgat cgataatgaa ggtgttgtaa aaatcacgga
     7921 tttcgggatt gctatcgctt tatctgaaac atcgatcacg cagacgaata cgatgctggg
     7981 atctgttcat tatctgtccc ctgagcaggc cagaggaagt atggcaacga agcagtcaga
     8041 tatttatgct ttagggatca tcctttatga gatgttgacg ggtagcgtac cgtttgatgg
     8101 tgaatctgct gtaacaattg ctttaaaaca ttttcaagat gatcttccat ctataaaagc
     8161 acttgatcca aatgtgccac aagcactgga aaacgtgatt ttacgggcaa cggctaaaga
     8221 acctgcagac cgttataagt cagccgagga aatgtctgat gatctttcta cagtattatc
     8281 accagctcgt gcaaacgaag aaaaatggca gcctcatgtg atggataatg aaacaaaagt
     8341 tatcacaccc cttacggaag atgcaccaat gcctgattca ttcaaatcta tgcctctgcc
     8401 aaaagacaag ccagaaaacg aaaccttgcc agaggaggaa gaagttctgc ctgatcctaa
     8461 aaagaaagga cagaaaaaat ggtggctgat cctattagct attcttgctg ttttaggaat
     8521 cggaacagct gtattctttg cttctggcgg gcgaggagaa gttgttgttc ctgatgtttc
     8581 tgatctttct gaagcttctg ctagagaaac actcgcaaaa gcgaaattag aagtagcaac
     8641 aaaaacagaa gaaattgcag atgacaaaat cgaagaaggt aatgtagtga agacagaccc
     8701 ggcagcggga acgacagtca agcaaaaacg cgaagtcact ctgtatatta gtagcggcac
     8761 gaaaaaaatc aaattagatg attacagtgg taaaagttat gaagatgccg aagcaagttt
     8821 gaaagaatta gggttttctg ctgacttgat tcaaaaagaa gaggaattca gttcagaggt
     8881 agaaagcgga atgatcattt cgcaatctcc atcagaagat acagaagtcg atcctaaaac
     8941 agacacgatt actttcacag tcagcaaagg accagaaaca gtgactttga gtagtcatgt
     9001 aggagaaaac tacgaaactg ttcgtaattg gcttgttaca caaggattca atatcaataa
     9061 tattagtgct acttatgact ataactatag cgacactgta ccatcaggtt cgatcatcag
     9121 tcaggatcca gcctcgggtg aagttgtagc agaagaaact tacatcaatt ttgtcgtaag
     9181 ccaaggaaca gagcctaaat tgaatgatat ctccggctat acaaaaagcg aagcacaaag
     9241 ttatttagct agtgtcggtg cagaatatat cgggcatgag acttccgaat attcaaacac
     9301 ggtggataaa gataaagtga ttcgtacagt accaggggca ggaacgacaa ttaccaaagg
     9361 aatggttgtg aacgtaattt actctaaagg accagatcca gcgtcttcta gttctgaaaa
     9421 agactcttca gacagttcta gcagctcaag tagctccagt agcacaagca actcttcaaa
     9481 agaagaatct tccagctcca caagttcatc agctagcacc tcagaaagtt caaaataagt
     9541 gctgaataaa atatatcaga agggaatgcg acattgattt gttgcattct cttttttgtg
     9601 tttctatctc ttttgatcaa ttttcaagcc atatactttg agaagtttgc atggaataac
     9661 acctactttc ctcttcgtat ggtaaaataa acaaaaacgg aggtggcact tatcaaagga
     9721 caaatcagaa aagcaatcag cggtttttac tatgtatacg caaacggaga gacctatcaa
     9781 acaagaggaa gaggaaattt ccggaatcgg aaaatcacgc cgcttgtcgg agatcaggtg
     9841 atctttgaga gtgaaaatca aacagacggc tatcttctgg aaatcttgcc gagaaaaaat
     9901 caattagttc gcccaccagt cacaaatgtt gatcttggaa tcattgtcac aagtatcgta
     9961 gagcctgcat tttcttataa tctacttgac cgctttctag ttaccttgga atacgagtcg
    10021 atcgaaccga ttattttttt gacaaagacc gacttgtcag acgacccaga aaaaatcgaa
    10081 gagatcaagc aaacctatac taaaataggc tatccggtga ttgtacccaa tcatacgggt
    10141 gatacgacag aactgatccg ttacttccca gaacggctat cggtctttat gggtcagtct
    10201 ggagcaggaa aatcgacgct actgaatcaa atatcaccgg aactgaattt agcaacagga
    10261 gagatttctg atgcacttgg aagaggacgg catacaacac gtcatgtgga actgattcct
    10321 atctatggcg ggttagtagc agatacacca ggatttagct cgattgactt tttgacaatt
    10381 gaagcaggag aactgccgag acaatttcct gaatttgtag aagcttctgt ccattgcaaa
    10441 tttagagagt gcatgcatgc taaagaacct ggctgcgaag taaaacatag agtagaaaca
    10501 ggtggtatcg cacagacacg ttacgacaat tatttgcaat tcttacaaga aatcgaaaaa
    10561 agaaaaccaa tgtatgcaaa aaaagaaggt aaacgaaaat aggaggaaac aagaatgaag
    10621 atagcaccat ccattttgag tgcagatttt gccaatttac aaagagacat tgagttagtg
    10681 gaaaaaggag gcgcagatta catccacgta gatgtaatgg atggccaatt tgtcccaaat
    10741 atcacattag ggccaaatat tgttcaggca atccgtcctg ttactaaatt accgttagac
    10801 gtccatctga tgatcgttga tccagaaaac tacattccag catttgcaaa agctggagca
    10861 gatatcatta ctgtccacgt cgaagcaact cctcatattc atcgggcact tcaaatgaca
    10921 aaaagcttag gagttaaagc tggagtagtt atcaatccag gaacaccagt ctccatgatc
    10981 aaacatgttt tgcctttggc tgatcaagta ctagtcatga ctgtcaatcc tgggttcggc
    11041 ggacaaagct ttattgaaga aacagtagaa aaaattacgg aactcgctga tctacgtaga
    11101 gagaacaatt ggacttatgc aatcgaagta gatggcggaa tcgttccaga aacagcaaaa
    11161 atctgtaaag atgcaggggc ggatgtcttc gttgcaggat catatatcta tggagccgaa
    11221 aatccagtcg gacaaatcga acgattaaaa gaggcattag actaaatgaa aattttatta
    11281 gctgctggag gtaatccgca aaactggccg cagtctctgc tgtccaaata cgatgcctat
    11341 gtcgggatag accgtggtgc gctgtacctg cttcaagcag gctacccagt agatctggca
    11401 gtgggcgatt ttgattcatt gtcgaacgaa gagcagaagg aagtatttgc aaaagccaaa
    11461 gagtataccc gttcccctgc agaaaaagac gatacagata cacagttagg tatcttaaaa
    11521 acatttgaac gttacccgca agcggaagtc actttgattg gagctacggg cggaagattg
    11581 gatcatcttt tagccaatct ctggttaggt atggaacctc gcttcaaacc ctttgcccat
    11641 cagcttatta tcagagattg tcaaaattat ttgtcttatt atttgcctgg agagcatata
    11701 gtaagacaga taccagatat gaagtattta gcttattgct gcttgactcc cgtctcgaat
    11761 cttacattaa aaggcagcaa gtatgtattg gaaaataaag aagtagaata tccgacatct
    11821 tatgcaagta acgaatttct ttcagatacc gcagaatttt ctttttcaga tggtatgatt
    11881 gcagttattc aaagcaaaga ttgatccata aacaaataaa aaaatttgtc tttcaggcta
    11941 aaaatgacag aatctattat ttgctaaaaa aaagcttaag aggagcaact taccttttgt
    12001 ttcacggcat ttagtgataa aattgaacac aagagtctaa ttaattcgag gtgtctttgt
    12061 gaagaagttc aatcctaata aaaatattat tattaccttg gttattgtta tcatcgtggt
    12121 gacgatacta agtgtgaccg ctgcgcagcg agcgaatgaa ggaaagccca atttcttcca
    12181 atccatcgtt aatgacagtg ttgcaatggt tgataaaaca atctctgccc cagtacggtg
    12241 ggtacgaaat ggagtagatt ctgtccataa tcttttttca acttatgccg aaaatgagcg
    12301 gctaaaagaa aaaatcgata gctatgatga cgtttcacag caaaacaaga attacgagag
    12361 agaaatcgaa gcgttgaaaa aagaacttga gttgaatcag acgctgacta gttacgaaaa
    12421 agtaacggca aatgtcatta ctcgttcgcc tgacagttgg caggacatgc tgatcgtaga
    12481 caagggttct aaagacggta tcgaagcaaa tatggcagtc atgtcgcaaa aaggattgat
    12541 tggccgagtg atcgaggtca atacggcttc gtctaaaatc gaattactgt catcctctaa
    12601 tgaaagctcc aatcattttc cagtacgggt atcttcggct aatggcgaag cgtttggttt
    12661 gcttaaaaac tatgatgaaa agctccatgc cttagtggtg acccaattaa ctggtgatac
    12721 ggatatcaaa gaaggggatg ttgtccagac atccggtctt ggagggaatt ctccagctaa
    12781 cttgccgatc ggtacggtta ttaaaacgaa accagatagt tatgggctgg atcgggaagt
    12841 ttatgtgaaa ccttatgcag aaatgtatga cgtgtcagtt gtgacgattg tccaacggct
    12901 agcggaggat tagtccatgt tgaagaaaga aacaatgaag tactatctgc caatcgtttt
    12961 gttctttttg atgttgatag atggtcattt aacaagaatg ctaggggagt ggtcgaaagg
    13021 cacctatatg tcaaatgccc actttctgat attggcatta ttatgttgca gtatggcgtt
    13081 tgaaaaacgt tatttactga ttaccacgat tgttctcggg gctatctatg acgcttacta
    13141 tattggcgtt atcggtatct atgcagtagc tctcccttta attgtatggt tgatgtatgt
    13201 aatgaaagac gttatccatg tcaacatctt tactgaattt ttcagtatga tcatctttgt
    13261 cacgggttat gaattgttta cgatggtggt ccagttgatt tttaaattag cagtagtaaa
    13321 taacacgtat tttattacaa ggtttttagg acctacact
//
LOCUS       DAYYHX010000064        13303 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000064 DAYYHX010000000
VERSION     DAYYHX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.064"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..2377
                     /locus_tag="ACHA6Y_001396"
     CDS             5..2377
                     /locus_tag="ACHA6Y_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM8987498.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="ACHA6Y_001397"
     CDS             2438..3898
                     /locus_tag="ACHA6Y_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM8987499.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="ACHA6Y_001398"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="ACHA6Y_001398"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8987500.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="ACHA6Y_001399"
     CDS             6129..6278
                     /locus_tag="ACHA6Y_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987501.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6353..6946
                     /locus_tag="ACHA6Y_001400"
     CDS             6353..6946
                     /locus_tag="ACHA6Y_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM8987502.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="ACHA6Y_001401"
     CDS             6959..7858
                     /locus_tag="ACHA6Y_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM8987503.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="ACHA6Y_001402"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="ACHA6Y_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8987504.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="ACHA6Y_001403"
     CDS             8724..8984
                     /locus_tag="ACHA6Y_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987505.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="ACHA6Y_001404"
     CDS             9429..9635
                     /locus_tag="ACHA6Y_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8987506.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9656..9886
                     /locus_tag="ACHA6Y_001405"
     CDS             9656..9886
                     /locus_tag="ACHA6Y_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987507.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="ACHA6Y_001406"
     CDS             9902..10339
                     /locus_tag="ACHA6Y_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987508.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="ACHA6Y_001407"
     CDS             10332..11039
                     /locus_tag="ACHA6Y_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987509.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11203..11403
                     /locus_tag="ACHA6Y_001408"
     CDS             11203..11403
                     /locus_tag="ACHA6Y_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987510.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11419..11598
                     /locus_tag="ACHA6Y_001409"
     CDS             11419..11598
                     /locus_tag="ACHA6Y_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987511.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11923..12186
                     /locus_tag="ACHA6Y_001410"
     CDS             11923..12186
                     /locus_tag="ACHA6Y_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFM8987512.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12180..12530
                     /locus_tag="ACHA6Y_001411"
     CDS             12180..12530
                     /locus_tag="ACHA6Y_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8987513.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12554..13168)
                     /locus_tag="ACHA6Y_001412"
     CDS             complement(12554..13168)
                     /locus_tag="ACHA6Y_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8987514.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         4922 a         2198 c         2737 g         3446 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga aaaggataat taagtatata cgttttttct
    11881 tatgtatgtt atactatgta tatacagaaa gaggtgtcta ctatggaaat gttacctata
    11941 aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt atattatgga atatcttgga
    12001 attcatacag aagacaaagt gaagataata caagaagaag taaacggtca gaagcggcta
    12061 attatcgaag cggctaatat agaaaatgaa ttaacaattg aacagttatt tgaaaattac
    12121 actgatgaaa gaaatcatgt gacgattcaa aatttaggtg aagcagtagg taatgaaaaa
    12181 tggtgagaaa acctagacaa ggcgatattc tattattgaa tacagcacct cgatctggac
    12241 acgaacagac aggtaaaaga ccttatattg ttttaagtca tgatattatt gcggattata
    12301 gtaacgttgt tacagttgct ccaatttcta gtacaactag aaactatccg ctatatgtaa
    12361 atataaactc agaatatgaa atgaagacaa caggaaaagt attattagat caactaacga
    12421 caattgatta tgaagccaga gagtgtattt ttttggaaaa agcgcatgat agtttagttg
    12481 aggagttatt gatgaaagtt agaacagttt ttcaaaaagt aagcaaataa aatagaaaca
    12541 gcagcatgat caactattcg tgattgacca tgctgtcttt ttttattcta tatacggttt
    12601 gtctcgtaac attgtagtct tttgcaatct tagcgatcgc aacacctttt tttagatcct
    12661 caacgatgtt tttataaaca agacgacgtt ggggatcttt tgcattggca ctgtatagct
    12721 taggacgtcc tttataaaca ccgttcgctt ttgcaatctt aatgccttgt gcctgacgtc
    12781 gtttagattc ggtacgttct tgttcagcga tcatggccaa aatttgaatg atcaaatctt
    12841 taatgaattt atccaggaga ggattgccaa ttgcttcggc catgatcggt agacttgtga
    12901 tgatcagctg cacatttttc ttcttcaagt aattgactga agcgatgatc tcgtcgtaat
    12961 ttcttccaag tcgatcaata gcttccacaa taaaaatatc ttgatctcgg acgaaatcca
    13021 gtgcttcttg aaaaacaggt cgatgagtaa tcgttgcgcc agattgtttc tcaatgaaaa
    13081 ttctctctgc accgaatttt ttcatttctt caatctgccg ttgttcattt tgatcgatgg
    13141 atgagacacg tatgtatgca actctcaaaa tttttaccta ctttctgcaa ctatttaaaa
    13201 aacaccctaa tcatacacga gaaaaccaga tttttcaatg tcgcggtcgt gtctagttag
    13261 ggtatacctt atatagacaa aaactaatga tggaggaaat agt
//
LOCUS       DAYYHX010000065        12992 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000065 DAYYHX010000000
VERSION     DAYYHX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.065"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      130..296
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            444..1001
                     /locus_tag="ACHA6Y_001413"
                     /pseudo
     CDS             444..1001
                     /locus_tag="ACHA6Y_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1059..3098
                     /locus_tag="ACHA6Y_001414"
     CDS             1059..3098
                     /locus_tag="ACHA6Y_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8987515.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3207..4358)
                     /locus_tag="ACHA6Y_001415"
     CDS             complement(3207..4358)
                     /locus_tag="ACHA6Y_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8987516.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4512..5042)
                     /locus_tag="ACHA6Y_001416"
     CDS             complement(4512..5042)
                     /locus_tag="ACHA6Y_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8987517.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5141..5491)
                     /locus_tag="ACHA6Y_001417"
     CDS             complement(5141..5491)
                     /locus_tag="ACHA6Y_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM8987518.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5648..7177)
                     /gene="ahpF"
                     /locus_tag="ACHA6Y_001418"
     CDS             complement(5648..7177)
                     /gene="ahpF"
                     /locus_tag="ACHA6Y_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM8987519.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7187..7750)
                     /gene="ahpC"
                     /locus_tag="ACHA6Y_001419"
     CDS             complement(7187..7750)
                     /gene="ahpC"
                     /locus_tag="ACHA6Y_001419"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM8987520.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7894..8556)
                     /locus_tag="ACHA6Y_001420"
     CDS             complement(7894..8556)
                     /locus_tag="ACHA6Y_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8987521.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8740..10386
                     /locus_tag="ACHA6Y_001421"
     CDS             8740..10386
                     /locus_tag="ACHA6Y_001421"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8987522.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10377..11459
                     /gene="uxuA"
                     /locus_tag="ACHA6Y_001422"
     CDS             10377..11459
                     /gene="uxuA"
                     /locus_tag="ACHA6Y_001422"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8987523.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11567..>12992)
                     /locus_tag="ACHA6Y_001423"
     CDS             complement(11567..>12992)
                     /locus_tag="ACHA6Y_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8987524.1"
                     /translation="MSESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPA
                     YCYYRLFGRYCNESVSYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLAL
                     YGLSISFSNNGRKSFSGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFK
                     KEVLDRLNSGMIDKLYFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPN
                     LPICREQRILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFD
                     LFIPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYM
                     DFREQNQHLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVK
                     IEQIFSNESIKIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRI
                     SEYIFELKEEALMDQSKKENQVTS"
BASE COUNT         4341 a         2386 c         2411 g         3854 t
ORIGIN      
        1 gattctttca acttatttgt tatttttttc taaaaaatat taaagattgt taaatttcac
       61 tatttcattt caaattccat tgcctaatca ttaggaaaag tctatcatga actcgtaaat
      121 agaatatagt aataaccttg ttaggtgagg ctcctataca aacataggct gttgcccaga
      181 aacgtcgaaa gtcgccaatg ggtaaaacag aaactgttgg attaaggctt tttccaatag
      241 ctaagaatgt tttctttacg ttgtatagtg ctaaaactcg gacgatgaga tgatgctagg
      301 tcagaattct tgttcgaaaa ggtttctgac cttttcttta tatttttgtg actgacttat
      361 ttgatgagac aaactcgctc ctcacctgac aagtggattg ctgtgtgagg aggagatgcg
      421 gtgaaaaaag atcgattggt ggaggtcgct cggattcact tctggttgca caatacaacc
      481 agaaaacaca acaagcaaaa ctgatctcta tcatgagaga ttcatatgtc gatattccag
      541 gttacggaat gaataagata aatgcagctt actcttatgg gggagttgat ttattaaatc
      601 aaacattaaa ggaaaatttc aaatttgaag ctccctatta tgcaagtatc acatttcaag
      661 attttatcga ttgcgttaat gaactgtttc ctggtggagt caagatcgat gcagaaaaat
      721 ctctagattt agatggcgta tatataaaca aaggagagca agtgatggat ggtaatactt
      781 tactccagta tgctcgattt cgggaagaca aagaaggaga cttcgggagg atcagaaggc
      841 agcagcaagt gatcaaagct atctctcagc agctgaaaga cgtaacttca atattcaagc
      901 tacccaaagc ggttgggaaa ttacttggta gtatccaaac gaaccttcca gaaagtgtgc
      961 ttctggattg tggtatggat ttcctcaaag acatatgtta gaataaacag gtataaatag
     1021 tgaaataaag gaatgacaag caagagaagg aggaaaaaat gaaaagaagt gacaagcacg
     1081 gcaaaaatcg aacaggcgct tatattgccg gcgcagtgat cttaatagca gctgcgggtg
     1141 gcggttattt ttactaccag cactatcaag aaacccaagc agtagaagct ggagaaaaga
     1201 cagttgagca atttgtccaa gctttgaaca agggagatta taacaaagct gcagaaatga
     1261 catcaaaaaa ggcggcaaat aaaagtgcat tatctgaaaa agagatctta gataaatacc
     1321 agaatatata cggtgctgct gatgtcaaag gacttcagat atcgaatcta aaagtagata
     1381 aaaaagacga ttctacttac agtttttcat ataaagcaaa gatgaatacc tcattaggtg
     1441 aattgaaaga tctttcttat aaaggaacat tagacagaaa tgatggtcaa accacgatca
     1501 actggcaacc caacttggtt tttccagaaa tggaaggaaa tgacaaagta agcctgacca
     1561 cacaagaagc agcaagaggg aatattatag atcgaaatgg tgaaccatta gcaacaacag
     1621 gcaaactaaa acaattagga gtcgttccaa gtaaacttgg agatggaggc gaaaaaacag
     1681 ccaatatcaa agcgattgct tcctccttcg acttaacaga agatgctatc aatcaggcga
     1741 tttcacaaag ctgggtacaa cccgattact ttgtcccatt gaaaatcatt gatggagcaa
     1801 cgccagaact tccagctgga gctaccattc aagaagtaga cggcagatat tatcctttgg
     1861 gtgaagcagc tgctcagctg attggttacg tgggagatat cacagcagaa gatattgata
     1921 agaatccaga attaagcagt aatggaaaaa tcggtcgctc aggtttggag atggcttttg
     1981 ataaggatct tcgtgggaca acaggtggga aattaagcat tacagatgca gacggtgtag
     2041 agaaaaaagt tctgatcgaa catgaagttc aaaatggcaa agatatcaaa ttgacaatcg
     2101 atgcgaaggc acaaaaaata gcttttgaca gtctaggagg aaaagctgca tcaactgttg
     2161 cgacaacgcc aaaaaccggt gatcttcttg cgcttgctag ctctccaagc tatgatccaa
     2221 acaaaatgac aaacgggatc tcacaagaag attacaaagc ttatgaagaa aatcctgaac
     2281 atccattcat cagccgattt gcgacaggtt atgctcctgg atctacgttt aaaatgatta
     2341 cagcagcaat cggtctcgac aacggcacta tcgatccaaa tgaagtgttg acgatcaacg
     2401 ggcttaaatg gcaaaaagac agttcttggg gatcttttaa aacaacgcgt gtcagtagtg
     2461 atgtatcaca agtagactta aaaactgctt tgatctattc cgataatata tatgcggcac
     2521 aagaaacgtt gaaaatgggt gagaaaaaat ttcgtacagg cttagataaa ttcatttttg
     2581 gtgaagacct tgatttgcca attagtatga atccagcaca aatttctaat gaagatagct
     2641 ttaactcaga tatcttgcta gctgatactg gatatggaca gggcgaactt ctaattactc
     2701 ctatccagca agcagcaatg tattctgttt ttaccaacaa tggcacactt gtctatccta
     2761 aattgattgc agataaagag acaaaagata agaagaatgt catcagcgaa acagcagtac
     2821 aaacgatcgt gccagatctg agagaagttg tgcaagatgt aaatggtaca gcacattctc
     2881 tttctgcttt agggattccg ttggcagcga aaactggtac agcggaaatc aaagaaaaac
     2941 aggatgtaaa ggggcaagag aacagtttct tgtttgcttt caatcttgat aaccaaggtt
     3001 atatgatggt cagcatgttg gaaaataaag aagatgatga ttcagcaacc aaacgagcat
     3061 cggaactatt gcaatacctc aaccaaaatt atcaataaaa aattgctttt taagtacagt
     3121 aaaaggactg tggcgttatc gtcacagtcc ttttttcaaa taaacggttt tcacgatgtc
     3181 tctttcacaa cttctttttg atttactcac acttcataaa ctttttttgt aaaatatttg
     3241 agaacaagtg gagaaataag tgtacttaat aaaatcacga taactaaagg tgaatagtac
     3301 tgtggttcga tcaattgact ttgctgtcca atttgtaaaa tgatcaaagc catttctcca
     3361 cgcgaaatca taccagcacc gaccatcaat gaactatgtt gagaaaaacc ggcaatctga
     3421 gcaccaaaat aacctccaag caatttggtc aaaatcgcta caagcgtcaa tatagaaata
     3481 aagagtagtt gttcactaaa gtcagaaaaa tcaacttcta atcctacact gacaaaaaat
     3541 actggtataa atactgcata tcctaaagcc tctacattgt ggtatacttc ttctttaacc
     3601 tttgtctgac tgacagctat cccagcaaaa aaagcaccaa tgacagagct aagcccaaca
     3661 agatccgcta cgtaagacat tcctaaacaa ataactagcg atacgatgat aatagaagaa
     3721 tttgcgtaca ctttttctgc aaatgaaagt aggaaaggcg caatccattt gactaataag
     3781 aaaataaaaa caaaataaat tagctgttca atcaaaacaa gcggtaaaga agcattgcta
     3841 gaagcttccc ccgttagaaa agataggctg aaacttagaa ctaaaacgac taaaatgtca
     3901 tctaccacag aagcacccaa aattgttgat ccttcttttg tattcactac gtttagttct
     3961 tttaatactt ctactgaaat actgacagaa gttgccgcaa gaataatccc aaagaaaatc
     4021 gcttcatttc ccgaaacttg gaatgctttg cctgtcaacc agccaaaaac aaccgggaag
     4081 atgatcccaa gcaaagcaac gaacatacca ggtcggaaat attttttcaa aagagataaa
     4141 tcactttcca gaccagctaa aaacattaat aagatcacac caatctccga aaagtcatgg
     4201 actagaatat caggtttgac ccaattcaat ccagcttggc ctagcaaaac accaacaagt
     4261 aattgtccaa tcactgctgg gatacctaat ctccgcgaga tatgtgagcc aatcgttgtt
     4321 gaaatcaata tcaggcataa aataccaata aattccattc tcatcattcc tttgtataaa
     4381 atatagcccg aacttagcat aacatacatt attgaaaaaa taggaaaata tactcacaaa
     4441 aaaaccaaca atccaaaaga tttttttgga ttgttggttt ttatacggtc tattttaata
     4501 actaaaaatg attaacgtat agtcattttt cttattctga tgaaattgat aatctcctgg
     4561 atgcccatga aaatcaaaat aaagccgaat atttggaata gaagcatcaa acttttgaat
     4621 ggattgaaca ataagacgaa tccgccaatc aacaacagaa tactactata aacactccaa
     4681 cttttagctt ttgtattcat gctgaagaac aactggaaca agctgttgat gataacagct
     4741 aatccaagaa gaatcggcag gatagaaacg attgcaggtg caaaagcata gacgatcaga
     4801 gctaagatca ggaaaaaaag accactgaac agaccgatac tccaagaacc tgttttcctt
     4861 ttaaaacggt aagcctcaaa aagattcaac aacccaagca aaacaagata ggcggtgatc
     4921 agataaccga taaaatgaaa aacagcagaa gggtttagca caatcgcaat cccagaaata
     4981 atataaacag cagctcttaa taaggcgtat ttttgaatat tttgtttaat cgtctcaaac
     5041 atataagaaa acctcctttg acttatttta acgtgaacta gtcattttac caaattaaat
     5101 gttacaaaat gagcgaagga gaagaatatc tctggaaaaa tcaacttctg aggatctttt
     5161 ccattttttt gccttttgct agctcatcga tcattttatc taaataacga atttctttca
     5221 tcaagttatc ctcgatgtct tctactcgta caccacaaac aacacctgta atcaaggaac
     5281 gtgaaggatt ggggacaggg gcttccgcaa aaaattgttc gaaagtcact tgtttttgta
     5341 gttgatattc tagttctgtt tgggaataac ccgtcagcca acaaatgatt tcatctactt
     5401 cctcttttat acgatctttt ctctccgctt ttgctacata cataggataa acgcttgcaa
     5461 aacttgtcgt aaaaatacga ttttgcgcca taatcagtca cctttctttt tttcaaatta
     5521 tttactactt atagtatata agaaaaagag aagaaaaagc gattattcaa aaaaacaaaa
     5581 aggattgtga caaaattctt gtcaccatcc ttttttccgc tctctgactt tttacaatct
     5641 tcatagctca agtgcgcatc agataatcga atgctcctaa agctgcagta gctcctgaac
     5701 ccattgaaac aatgatttgt ttgtatggac tatctgtaca atcaccagct gcgaaaacgc
     5761 cggggatatt cgttgcgcca tttttctcgg tcactatttc atttcttgga ttcatttcga
     5821 tcgttctatc tagccaatcc gtattaggga gtaagccgac tagaatgaat actcctgaaa
     5881 cagctagtgt atgcatctcc atcgtacggc gatcgatata ggagagagat tctacctgat
     5941 tcgtaccgct gatttctttc gtcgctgcat gtttgataac agtgacattc gcaagagaga
     6001 ctagtttatc ctgtaagatt tgatctgctt ttaattctgg taagaattct agaacatata
     6061 catgttttgc taacccagcc aaatcgattg ctgcttcaac accagaatta ccaccaccga
     6121 taacagcaat ctcttttcct ttaaataaag ggccgtcaca gtgtgggcaa taagcaattc
     6181 ctttattttt aaactctttt tcgcctggta cattgataga acgccagtgc gcacctgtcg
     6241 cgatgatggc ggtttttgct ttaagtgata taccgttagc taagccaata ttaataaaat
     6301 gttgctcttt atttaaggaa acggcctgtt gattcatcat aatgtcgata gggtaactac
     6361 gcacatgact ttctacttgc tgcatcaatt ttggaccttc tgtgtaagag gtaccaatca
     6421 tattttcaat ccctagtgtt tcaacgactt gtccgccgaa agaatcagcg acaagtcctg
     6481 tacggattcc tttacgtgct gcatagatag ctgcactact agctgctggt ccaccgccaa
     6541 tcactaatac atcataaggt tctttttcaa acaactcttc ttcaatcaaa gggccagtga
     6601 ttttttcaac aatttgttcg atcgtcatgc gtccgctact aaattcttct ccatttaaaa
     6661 acacagttgg cactgccatg atttttttat catcgatttc tttttggaac atcccacctt
     6721 caatcatcgt atgagtaata tttggattta atacagctaa tatattcaat gcctgtacaa
     6781 cgtcggggca attatgacaa gtcaagctaa cataactgtc aaaatgcaat gtttgtgtta
     6841 tttttttgat tttaagactc aaagattcac tgattttcgg tgcccgtcca ctaatttgta
     6901 acatagccag taaaaaagag gaaaattcat gaccgagggg gataccagca aaaacgattc
     6961 cactttcttt gccaactgga ttgatagaaa aactaggtgt gcgcatcgaa tctctttttt
     7021 cccaagagat tttaggagac atttctgcta tttccttcgt aaattcagct agtttttgac
     7081 tattttcact attatccaaa ctaacagaaa aaacaatagg agattcaatc agtgctaaat
     7141 attgttcgag attttgttta gtttctttgt ctaacatatc caccacctaa atctttccaa
     7201 caagatcaaa acttggtttc aatgtttcgc cactttcttt ccattttgcc ggacaaactt
     7261 cacctgggtg ggtacgtaca tattgagcag cgcgaatctt atcaatcagt gcactagcgt
     7321 cacgaccgat tccatctgca ttgatttcca tagcttgaac gattccgtct ggatcaataa
     7381 taaatgttcc tcgctgagct aatccttgct cttcatccaa aacatcaaaa atacgagaaa
     7441 tctgatgaga cggatcaccg atcatcacgt attcgatttt accgatagca tctgatgtat
     7501 catgccatgc tttgtgagta aagtgggtat cggtagagca agaaaagact tcaacaccta
     7561 aagatttcaa tgtactgtat tgttcttgta agtcttctaa ttcagtagga caaacaaaag
     7621 taaaatcagc tggatagaag caaagaatac tccagttccc cataagatct ttttcactga
     7681 cttctaaaaa ttctccttga tgataagcat gtgcattaaa ttcttcgatt tttttcccaa
     7741 tcaatgacat agtaaaacct ccaataataa ttatttattt ataattattg taatttagtt
     7801 ggagttgcaa agtcaatgga tattttgtta tttaataaag attactcaaa aacgaaggtt
     7861 aattataaaa atagaagagg tcgcaagaaa atgctactgc tggaaatact cgggataacg
     7921 ttgagtaaca gtttcctgtt ctttcagcat catgtgtaag tgcaatcccg tttgaaatcg
     7981 agcttcttct ggttttttat caactaaagc cttgagtaaa tcataatgtt gttccatgat
     8041 cgtttcccat ggaagcccct gaatggtcac tcgtaaccag cggaaacgtt ctaaatgggt
     8101 attatggtca tctaaccagt tccaaacttc ttttcttcct gcaatttcga aacaagcttc
     8161 atgaaataaa ttatctgcac ggaaaaaagc gcgttcgtca tttattttga ctgctttttc
     8221 ttgttcttct aaaatcgttt ctagcactat tcgtgtttgt gtgtccaatt tggaacaaca
     8281 ttcaatcatg atttgctctt ccaactgttc tcggacaaag cgagcatttt ctgcagaaac
     8341 aagattaatt ttggaaacat acgtaccact ttgaggtatc gtatacacga gttcttgctg
     8401 acgtagttga atcaatgctt cgcgaatggg agtccggccg atagcaagat gttcctccag
     8461 tgccttttca gatattttcc ttccaggttc taagtcagca tagataattt gtttacgaat
     8521 cgcttgataa gcttgtgatt gcaagctgct tttcatcttt attcctccaa aatcatttct
     8581 atgaacgtag tataacagaa gaggtctgaa aaacaatctg gtatgtcaca ttaactagta
     8641 aatttgtata aatagtaaaa ttttggttga catagcgatt tttattgcat aaaattcact
     8701 tgaatacaaa aactaatatg ttagtttagg aggggaaaaa tgattgattt aaacaactgg
     8761 aggaacttta tccctgaatt atctcaaaaa gaagtagaaa tacctcattt tgaccgtgca
     8821 tcattaagag aaaatggaca aaaaaatcct gtctggatcc atttcggtgg cggaaatctt
     8881 tatcgaggat ttcatgcaga aatcgctcaa cgtttagcgg atctaggcga attgtccact
     8941 ggtgtcattg tttgtgaaac attcgatgaa gaaataattg aaagggctta ccaagatttt
     9001 gatgatttga ttcttgaagt ggtcatgcca agcagtggag agttcgtcaa acgtatatta
     9061 gctgcgacag ctgacagtct gtattgtcat ccagattgtt ctgaacactt tgataagatg
     9121 attcgacact ttgaaaatcc tagtttacaa ttagttactt ttgctattac tgaaaaaggt
     9181 tatcgattga aagatacaaa taataaatat ataggaccga ttgcagtcga tataaaaaat
     9241 ggtccaaaga agccatcaca tacaatgagt attcttgctg cgctacttta taaacgtttt
     9301 ttatctggag cttggccgat tgcacttgtc tctacagata atttttcaca aaacggtagt
     9361 caattacgta cttctttgct aacgatcgca aaagaatggc aaaaggaagg ttatgtacaa
     9421 aatacattta tccaatactt atcgaatgaa gaaattgttg cgtttccatg gacgatgatt
     9481 gatcgtatca ccccgaatcc ttccttgtca gttagcaaaa tgttggcaaa agagggcttt
     9541 tcaaatatgt cgattttcca aaccgcaaaa ggaaataata ttgctccatt tgtcaataca
     9601 gaggacatcc attatttaat catggaagat cattttccca atggacgtcc gaaattcgaa
     9661 aaagctgggg tattcttgac tgatcgtgca acggtagata aagcagatac gatgaaggtg
     9721 acgacttgcc ttaatccttt gcatacgtct ttagctatta tgggttgttt actggggtat
     9781 aggagcatag ctgaggagat gaaagatcca gatttatcgc tgctagtttc tgaaatcggg
     9841 tacaatgaag gcctcccagt tgtggagtct ccaggtatca ttgatccaaa gatctttctg
     9901 gaagaagtaa ttactgtgcg cttttctaat ccaaatattc ctgatatgcc tcagcgaatc
     9961 gccacggata cctctcaaaa ggtggcgatt cgttatggtg agacgattaa aaaatatatg
    10021 gaaatagaag ttaaagaccc gcagacactt gtattcatac cattgactct cgctgcttgg
    10081 cttcgatacc ttattggcat tgatgatcaa ggaaaaccat tcatcccgag tccagaccca
    10141 ctattgacag actttcagca taagctttcc gatattcaac tgggtacgca agatacatca
    10201 catattcatg agaaaatggc tcctattttg tcaaataaag aaatttttgg tgtagatctt
    10261 taccaagcag agcttggcag aaaaatagaa gaaatgttca tcaagatggt agcaggaccg
    10321 aatgccgtac gaaaaacatt acaggattac ttacaagaac atggaggaaa tttatcatgg
    10381 aaatgacttt tagatggtac ggatcacagg aagaaaagat taccttagat gaaatcagac
    10441 agattcctgc ggtcaaaggg attgtaggca cattgatgga tataccagta ggagaggtat
    10501 ggcccaggga gaggatcaaa gaactaaaag aagaaattga gtctcgtggg ttaacactaa
    10561 aagtaattga aagcgtcaat gtgcatgatg ccattaaaat tggattacct tcccgtgagc
    10621 agtatatcga aaactataag cagacaatcc gaaacttggc agaatatgga atagaagtca
    10681 tctgctataa ttttatgcct gtatttgact gggtgaaatc tgaccttgac taccgcttag
    10741 aagacggctc atccacgtta gcttttatat ctgatgatat tccagatgat ccaagagaaa
    10801 tcgtcgcgcg catcgaacat tccagcaatg aatttgaatt gccgggatgg gaacctgagc
    10861 gcttagctca tatcaaatcc ttgcttgaag cctatgcatc agtagacgaa gaaaaacttc
    10921 gagaaaattt tgcctatttt ctccaatcaa tcatcccaac atgtgaagag gtaggggtca
    10981 aaatggctgt acatccagat gatccgccat actcgatttt tggattacct cgaatcgtaa
    11041 agaatcgaga agatttagat tggttgtgcc gcatagtaga cagcccttat aacggaatca
    11101 ctctttgtac agggagtatt gcagaagatc cagaaaatga tgtatatggg attttagcag
    11161 aattttgtca aagagatcga atcccatttg cacatgttcg aaatattaaa tttttagcta
    11221 atcgagattt ctatgaggca ccgcataaat ccgaatacgg ttcattagac atgtaccgca
    11281 tactaaaaac aatgtatgac aacggattta acgaatacat ccgtcctgat catggaagaa
    11341 tgatctggaa tgagacagga agaccaggat acggcttata tgatcgagca ttaggtgctg
    11401 tttatctaaa tggattatgg gaagcaatcg aaaaaggaaa caaaagtaaa gataattagt
    11461 gaaaaaaact gtaattttat tatgtaaata agaaggatgt tcaaattctt gatacataaa
    11521 tccggaggtt gttaataagc attcggattt atgtatctta tttggtctag gaagtaactt
    11581 gattttcttt tttcgattga tccatgagtg cttcttcttt taattcaaag atatattctg
    11641 agatcctcat aatcactttt ttccagtctt ctggtgtgat gattcctttt aaaatataga
    11701 tttttttgat atgagaaggc aattgtttca aagggcagtc gctgataacg atgttgcagt
    11761 tctccataaa atcattttgt attttgatcg attcatttga aaaaatttgt tctatcttaa
    11821 cgatcagatc tttactgata taaaaatcac gcgtatagtt cacaccaatc acaattcgcg
    11881 gctgtttttt gtagcgatca tacaaatgta ctaggtcttc aaccatccat tgcacatttt
    11941 ccttttctaa aataactggt aacaagtgtt gattttgttc tctgaaatcc atataaaatt
    12001 gttcaatggc ggtttggact tctttcaaca cgctatgttc ttgataaagc atgaaagtag
    12061 gaaggggcgt atttggttga ttgaagttaa gaaatccatt ataaagttta tttcgtgcga
    12121 atgccaatga gtaaagaatc ttttcttttt caggtataaa caaatcaaat acagtacaaa
    12181 aggactccac catttctctt gaataaagaa catgaggaac gtttgctttt tttaaaagtt
    12241 tgtattgctc caagcttgtt tcttccgatt cgattttcgt gatagaaagt cgagctaaaa
    12301 tattcactaa gatgcgttgt tcgcgacaaa ttggcagatt aggtcgcaaa atatctacag
    12361 agggatcaat aaaaaagtca atatattcat actctgtgaa ttctttttgt acgtctttca
    12421 cagaagttgc aggaaaattc tctttacata tttttaatag aaaatataat ttatctatca
    12481 taccgctatt tagtcggtca agtacttctt ttttaaaaaa agtattcatc ataaaagcca
    12541 ctacttgcgg atcttcatca taaaattctg tagcagtact tgaaaagatg ttccaataaa
    12601 tatctaatgc tgcatattga atttgtacct cttttccaga aaaagacttt ctgccattat
    12661 tagaaaagga gatagacaaa ccgtacaatg ctaaaaatgc attaatcttt ttcattttcg
    12721 tatacaaata agaggcacta tagtttgttt gctgacttaa actgataatc gaatttgtct
    12781 gacagctgaa caaagaagtc aagatctgat aactgactga ttcgttgcag tatcttccaa
    12841 atagacgata atagcagtaa gccggatcaa ttccttcaaa attcaacttt aaaaaaggtt
    12901 tttgcttatc aatatgcagt tctgttagat tgagacgctc aaagtcttgt tctaattctt
    12961 tgatgtatag taaaaaattt gattcagaca ta
//
LOCUS       DAYYHX010000066        12930 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000066 DAYYHX010000000
VERSION     DAYYHX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.066"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..1857)
                     /locus_tag="ACHA6Y_001424"
     CDS             complement(46..1857)
                     /locus_tag="ACHA6Y_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM8987525.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(1854..2219)
                     /locus_tag="ACHA6Y_001425"
     CDS             complement(1854..2219)
                     /locus_tag="ACHA6Y_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM8987526.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2382..2777)
                     /locus_tag="ACHA6Y_001426"
     CDS             complement(2382..2777)
                     /locus_tag="ACHA6Y_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8987527.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2795..3757)
                     /locus_tag="ACHA6Y_001427"
     CDS             complement(2795..3757)
                     /locus_tag="ACHA6Y_001427"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM8987528.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3757..3969)
                     /locus_tag="ACHA6Y_001428"
     CDS             complement(3757..3969)
                     /locus_tag="ACHA6Y_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM8987529.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(3990..4688)
                     /locus_tag="ACHA6Y_001429"
     CDS             complement(3990..4688)
                     /locus_tag="ACHA6Y_001429"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM8987530.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4708..5250)
                     /locus_tag="ACHA6Y_001430"
     CDS             complement(4708..5250)
                     /locus_tag="ACHA6Y_001430"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM8987531.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5382..6386)
                     /locus_tag="ACHA6Y_001431"
     CDS             complement(5382..6386)
                     /locus_tag="ACHA6Y_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8987532.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6383..7372)
                     /locus_tag="ACHA6Y_001432"
     CDS             complement(6383..7372)
                     /locus_tag="ACHA6Y_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8987533.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7369..8175)
                     /locus_tag="ACHA6Y_001433"
     CDS             complement(7369..8175)
                     /locus_tag="ACHA6Y_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8987534.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8341..9297
                     /locus_tag="ACHA6Y_001434"
     CDS             8341..9297
                     /locus_tag="ACHA6Y_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8987535.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9374..9892)
                     /locus_tag="ACHA6Y_001435"
     CDS             complement(9374..9892)
                     /locus_tag="ACHA6Y_001435"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8987536.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9980..10129)
                     /gene="rpmG"
                     /locus_tag="ACHA6Y_001436"
     CDS             complement(9980..10129)
                     /gene="rpmG"
                     /locus_tag="ACHA6Y_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8987537.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10357..10803
                     /locus_tag="ACHA6Y_001437"
     CDS             10357..10803
                     /locus_tag="ACHA6Y_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8987538.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(10998..12893)
                     /gene="asnB"
                     /locus_tag="ACHA6Y_001438"
     CDS             complement(10998..12893)
                     /gene="asnB"
                     /locus_tag="ACHA6Y_001438"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM8987539.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4003 a         2762 c         2207 g         3958 t
ORIGIN      
        1 cgcatgaaga ctttctaaaa aacagataag tcatcatgcg tatttttatt tgtcagtcaa
       61 tataagttgt gctattttat ctgcctgtct cagattccat ggatctgtat aaggtctagc
      121 tactgctgca gttagccaat ccacaccttc aacaggtatt ccatagcaat aaaagtcaga
      181 ctggatttcc cctgaagcat caatcacgcg attcgtacct cgctcaacat ctatagcttc
      241 tgaatgataa cttcctgtat tatctgaaaa tgaaaaaggg cgaacaagtt tgttttcctt
      301 caaatttttt atgacaggat tacttgaacg agaaaagctt gtttgtggca atcgagcttc
      361 aatcaaatag cgactgctga tttctttatc ttgtagagaa ataacatagc ggttgtgctt
      421 ttgctgaata tcaaatggcg gttcgatcaa atgaaaaata cccgcatcta tcagtgctgc
      481 taattctttc gtccgaatga ttggcggacc gatcgttaaa aagctattta gatgagtaaa
      541 ccatccccat agtatttctt tgtattcctt agcagaaaaa acgtgccatt caatagcctg
      601 ccggattgga tcacgccagt cttttaatgc atcaaatgtt gaagcaaatg ctcctgtgca
      661 attgcctttt tccgcttctt ttatctgccc ttcaataaat attcggctag cttctgaaaa
      721 gggctcattc tgataggcaa gaggagtttc taaatacgac caatcccatc tataagggat
      781 tagtgctgga tgctttccga gacactcctc tgaagagagg gagagaaaat catgctcaaa
      841 atttttcttt ggaatcggca agtgatattc ttcgatgatt ttcttgtaat aaaacaactc
      901 aacgtctttt ttcagatagt caaaaaatgt ttcgccagtc aaaaatcccg actgatgcca
      961 ttgctccagg ttttcttttg ttagtaaccg tggccatgcc attgctccgc cttgcttttg
     1021 atttcttcct cgagggtaat aagggagccc ccttcttgat ccactgtata caatcggttc
     1081 cttgcctgat ggatgataga ccagttttcc cttttcgttc tcgaagcgcc ctcctcttga
     1141 tactgtaaac aatccgacat aatcaaaaaa acttaagcct agacctctta aaaagacatt
     1201 ttctttaggc ggtatcttcg aaagatctac atctgcagga ttagaaggaa gctgatacag
     1261 taaaacattt ttctttgcgt agtctaccca cactctttct tcttctgtct catcattttc
     1321 ccagtgaccg cttgctaaaa caagctgatc gatagaatag ctttgctgat ctgtctgtaa
     1381 aagataacca tcttttcttt tgcttacttt ttttactaaa gaataaacaa actgaataga
     1441 agctggaaat tcttcttgca actttgagaa aaaccagcgt tgatagagtc cgtataaaca
     1501 tcgagtagat tgctgatctt tttccagacg attgatttct tctataatta gttgcttatt
     1561 tttggttacc gtctgttcga tataatctgt agcaggtcct ttactccatt gaaacaagtt
     1621 gggtccctgt gttaccaatc ctccactgga caatgtttca tctgtaaaaa gcgtcacttg
     1681 ttgagaaaca gaattcatta ataactccgg tggctgattc aaccgccaaa ctcgacctcc
     1741 aggaccattg ggatcgaaaa gaaaaagttc aagagaatgt ctgtcctgat tattatggag
     1801 aatcctttct gctacactta atcctctagg tccggcacca atgatcccta ttttcatttg
     1861 catgcctcca tttcgataaa ttttttcaat aaaacagcat ggttctgatg ttcatttttt
     1921 gcaccataaa caaaagtaat gactggatgt tctttcataa gttgactgag tttaaaaata
     1981 gcttgcttct tttcttcttc gaaaatcaat tcttgctggt atttctttgc aaatgaagca
     2041 aatcgttctg gtacatggtc aaaagttttg cgtgcttcat tactaggtgc aatttctttc
     2101 aaccacagat ctaactgcgc tttttctttt gaaattcctc gtggccagaa tctatccacg
     2161 agtattctat agccatcctc tttttcagca tcaaggtaaa cacgttttaa tttgatcata
     2221 gttgtttcct tctttctacc ttttcgctta gcttatatat gtagataata gcacaaaata
     2281 aaaaaaatga acggcacaac tatccattgc gccgttcatg tgatcttggt caaaatctaa
     2341 tgattgattc ggtttttaaa gctgtcatac aatcatctgt tttatacttt tttacttttg
     2401 atttttccag accatccgtc atatccaccc ttaagtaaat aaatctcttg ataacctgct
     2461 ttacgtaatt tatttgctgt acggatgctc aaacttttct tttgatcgta aatatagaca
     2521 ggctggtctt ttcgaataga tcctaatgaa tctttcaaaa cagtgtaagg gatgtttctt
     2581 gcacctaaaa tatgcccagc atcaaacaca tctttttcgc gaacatcgat tacttgtgct
     2641 tttctcattc cttctcgaaa ttcttcttct gtcaaagtcg ttgctgaacg tttggccatt
     2701 actcgtagat agacttccca aaggatgatt gcaagcacaa tcaatagcaa aatgatatta
     2761 attacccata aaaccatgta atgcctccgt ttttttaagc aaatttcaag gctaatgagg
     2821 cagcaccaat gacaccagct tcattaccaa gctctgctaa tttgattttt gttgattctc
     2881 ttacttgcgg gaaagtaaat tctttaaaat atttctctac acggctacgt aaaaattctc
     2941 ctgctgccga tactcctcca cctaacacga tacttgacgg atttaatgta ttccccaagt
     3001 ttccgcaagc taaacctaag tagaagcata cgcgatcaac aaccatcaat gcaaacggat
     3061 cgtcttcttg agccatttca aagatatctt tacttgttac ttcttctccg ttgtctagca
     3121 ttgctttcag ttttgaatcc cctgcgtatt cttctgccaa gaaacgacct aggcgtacca
     3181 cacctgtagc gctagataca gtttctaaac atccgcgttt accgcaagta cattcgaacc
     3241 cttcaggatc cacagtgata tgtccgattt ctcctccagc tccgctcacg ccgtgaatca
     3301 aatttccgcc agcaacgata cctccgccaa cacctgttcc tagagtaacg aatacaacgt
     3361 ctggatcgtt ttctccagca cctttccatc gttcacctaa agcagcaaca ttcgcgtcat
     3421 tatcgatagc gaatttgatt cctgttcctg cctcgatcag ttttttcaca aattgaactt
     3481 ctgtccagtt taagttatac gcaccaataa ccgttccagc ttcgctgtcc acgcttcctg
     3541 gggtacccat gccgattccg ataaagtctt cggctttcat attatataaa ttcaaacgat
     3601 gattgattga ttcaatgatt tcaggaacga tatgcttacc atcatccaaa atattggtat
     3661 cgatgctcca tttttgctga atctctcctt ctggtgttaa aattgcaaat tttgctgttg
     3721 ttccacctaa atcaatccca ataatcttct tgtccattac aaatttcctt tctttcggct
     3781 ttcttcaatt cggtgctctc tttttaatat gcgccacgct gtcatgtatg tatcatgatc
     3841 aatcaaacgg ctatcataca gattacgcaa ttcgatagtc attaattcaa tgtcatagat
     3901 gcgtgcaccc acatagataa agatgccaaa ttttttgaac agctgctgta catcatataa
     3961 gctctccatc tttaagcact tccttcatat tatacaagta aaccatattt ttttaaaccc
     4021 agtaaaatac atattacaaa aagaaataca aaaactagtg tagaaagaat cctttggtgt
     4081 atcgaatagc ttgtctgttg tttcggtaga cccaaaacat tccctaacac taatccgcca
     4141 attagcccac cgacatgtcc ccaaacatct acagaggtat ccagaagacc aaaaacaaat
     4201 gtcaacacaa taaataaaag aaaatgtctg actagctgct ggatcgctgg gttatgttta
     4261 aaatgaaacc ctaagataaa caacgcaccg aacaaaccga atatcgaagt acttgcacca
     4321 ccagacagaa cattcgcttc gttaaaagca aagcttgctg tatttcccat tatcccgctg
     4381 aacatataaa tcaagaaaaa acgccaatgg ccatagattg cctcgatttg ctgccccata
     4441 aaatacagta tcagtgagtt cattacgaaa tgcatcagtc caaaatggat gaagattggt
     4501 gtcactaaac gccaccattc attgaagtga acaagataag gaccatacat tccggcataa
     4561 taagggatct caagtcgcgg aaataattcc atcatgagga agaccgctgt ctgtatccct
     4621 aaaaaagcat aggtccataa cggtcgtctt aaccatgcac gcaattttat ttgctgttga
     4681 taattcatga aaacctccca tttcatttta gtcaataaac aattgttgga tcttctgatc
     4741 aaagctctcc ggttgccatt gttcattcaa ttgttcacga aaaacaaggc tacatgtttt
     4801 tttatgaaaa ttatttaaat accggtcgta ataaccaccg ccaaaaccga ttcgataacc
     4861 ggctctattg tagataatac ctggtacaat gaggagatcg atcatctccg gcaaatagag
     4921 cttctctgat tgtggctcaa gaacaccaaa actcgaacgt acaagaacgg actgttcatc
     4981 catttcataa aacgccatct gacgtttcgg caaagtttta ggtacaacta ttttcttttg
     5041 ttctttttct gctcgttgga taaccggctg tgtatccagt tcaaattcat tcgataaagt
     5101 cgtccctatc acttttgctg acttccagta ctcgctggcg aatagctgtt tatagattcg
     5161 ctgttctttt tcctttttct tgccactttt ggccagattt ttcagctttt cgattccttt
     5221 ccggcgtaat cttactttca attggtccaa aaactcaccc ttccttctct cataaaatgg
     5281 tataggaaaa aaagaaaaat agcaatcgaa ctttcaaata aatgcataaa tagaataaaa
     5341 agaagaccta gaaatgattg gccttccttt tttaatgaat ttcaactgtt cagcgcgtgt
     5401 ttttgtctga taaaaagtgc atacagaaaa tatggcgcac caaaaagggc aatcaaaatt
     5461 cctgtaggta cttccccact tggtagaatc actctagcaa ccatatctgc actaagcaga
     5521 aaaatggaac cgaacaaacc gctcagaaca aaggtccagt tattttcttt acgtacgact
     5581 agcttcgcag catgaggagc tagtagtcct acaaaagata aactacctgt caccgctaca
     5641 ctaccgcttg ccaaagcaac agctaacaac aaaaggattt tttgactttt agccacgtta
     5701 aggcctaatc cttgcgcgcg ttcttgacca aatgatagca atacaatcct ctgtgcataa
     5761 agtcctccta gcggaattaa gataaccgcc catggaagca atgcccaaac ataaggccag
     5821 ctcgcacccc agattgatcc cgctaaccat gcattcacaa atccataatt ttccttagat
     5881 aattggatcg tgcatagcaa tgttaatgca gaaaggccgg cattaacggc aataccggac
     5941 aataagattc gattcatacc caaaaagcca tttcgatcat aggccgcaag atagacacag
     6001 caagcagcta aaacaccacc tagaaaggct acaactagca atagaaacgg cagcgaatag
     6061 tgcgaaaaga aacctaaata aagcatcact aaaaatcctg ctccggcatt aatgcccaat
     6121 atactagaat ctgccaattc atttcttgtt actccttgca agatataacc agaaacgcca
     6181 agacaagctc ctgctaataa actgatcagt aatcgaggaa ctcgaaattc tctaacaatc
     6241 agcgccaaag aagatgattc tttccctatg aacgtggcaa aaagctgatc caggtaaata
     6301 aaaggcgtac caatcattaa actacctata gacaggatga caagtacaaa aagcaagaaa
     6361 tagaacgtcc aatatatttt tttcaccgtt gttccctcct tacttgtatc agtaaaaacg
     6421 gaacgccaat caatgcaatg actaatccaa aaggtgtttc aaaaggggga ttgatcatcc
     6481 tcgctgtaaa atccgccaac ataaccaatc caccgccaac taaaaaagag acaggtagaa
     6541 cataacgata gtccgttcca accattccgc gaacaactgg cggaaccaac agcccaataa
     6601 atgaaacagg tccgactaac gaaacggcaa tggctgctaa cactgcaact agacagaaaa
     6661 caatcaaccg cgttcgttta ggatgacttc caagagctaa tgcatgtgtt tcactcaaat
     6721 gaatcaaaga tattgctttt cctaatgaaa aagaaaccaa aacagcaagt atatataaag
     6781 gaaacaatac tgctaattgt ttccatgtga tattggcagc tccgcctaca aaccaaaaag
     6841 caagatcctg ctgcagatta aagaattggg taagtgcttg gctgatagca gtaaaaaaag
     6901 aactaatagc tacgccagct aaaatcagct gtaaaggatt catgcccatt ttcgaaaact
     6961 gcaaaaaacc aaatacgagc aaagttacag caaaagaacc tgccaatgac attagaaaca
     7021 cagaaaaatc agaagcttta ggccaaaaag caaaactaag ggccattgcc aacgaagcac
     7081 ccgcgttgat tcctagtaag cctgaatctg ctaaaggatt tcttgtcact ccttgcatca
     7141 atgcaccgct caatgcaaaa caactcccta caagaaatgc tcccgccaca cgaggcaaac
     7201 gaatatttcg tatgatttgg tgactttgtt ttgatctatc aaaatcaaac aacgcctgcc
     7261 aaaaatctgc tttttccaca gtcgctgcgc catttgaaag agcaacaaaa aaaataatca
     7321 aaagaacaaa acagaccaaa agcaggagtc tatttttctt catagtcctc ataattgatc
     7381 ctcctgctct tttggcaaca aatcataggt tagaaaaact ggtttatttg ttcttggaag
     7441 cgtgacaatc gttgcatcaa tctcaaatat ttttttcata cactcttttg tgatcacttc
     7501 ttcaggtgta ccttctgtga tcaacttacc gtttttcatt gctaataaat aatctgaaaa
     7561 acgtgaagca tgattaatgt catgaatcga catcaagatc gttgtttgtt tcatttgatt
     7621 gatttcagtc aataactgta ggatctctaa ttggtgtgcc ggatctaaaa atgttgtcgg
     7681 ttcatccaag atcaagatat ctgtttcctg agcaagcgcc atagcgatcc agacacgctg
     7741 ggtttgtcca ccagacagcg ttgcgatatc ccgcatacga aatccagcta gacctgtagc
     7801 atccaacgcc cactcaattg cttcatgatc ttgcgtgtcc atgccactga acagtttttg
     7861 ataagggtat cgtccgtaag atactacttc ttctactgtc atacctgtag ggtgttcact
     7921 agtttgtgct aataatgcaa ttttttttgc aagttctttt gaactgtatg tagagatatc
     7981 agagttattg acaatgatct ttcctgattt cacaggtagt atacgggtaa aacttttcaa
     8041 taaagtagat tttccacttc catttggccc aatcaaacta gtgattttcc cttcaggaat
     8101 cgtaacagac atgttttcaa tgattttttt gttttcgtag ccggctgcaa tgtcagctgc
     8161 tcttaatctc gtcatttcac ccatcctttc gctgaaaatc attctcaatt atgacgctgt
     8221 ttcaacattt cgtcaagcgc tactttcata tctatagcca aatgaagcgg ttgacagcgt
     8281 tcgaaatatc aggaataata accaatgaga aagattctca attatgttag gaggaaagaa
     8341 atgaaattaa aaacaaaagt attcacatta tctagcatat tgctgctggc tggagtactg
     8401 agcgcttgct ccccctcttc agataaagaa acagctacaa ctgccaattc gacgacttca
     8461 tccaatagta ctcatacagt cacagataca cttggacata aagtagagat tccaaacgcg
     8521 cccaaacgga tcatcggcag ttacttagaa gactatctga ttgcactagg ggaaaaacct
     8581 atcgcccagt ggactgttgg cagtggttcc attcaagatt atctgcaaga tgatttaaag
     8641 gatgtcccaa cgatttctta tgaccttcct tatgagaaag tgttaagctt tgagcctgat
     8701 ttgctgttga tttcttcttc cgctactgtc gaaggcggga aatacgaaca atatagcaaa
     8761 atcgcgccaa cttatgttgt aaaaaacgga acagatgtca catgggaaga acaactaaaa
     8821 gatgtcggca aagctttggc aaaagaaaaa gaggcagaaa aaatcatttc cgattatcaa
     8881 aaatccatca agaaaactcg agaagaacta gcagataaaa tcgagaacaa gacagctgca
     8941 gtgttgtggg tgaccaacaa ctcagccttt atggtcagca aaaatcggtc aagtggaaga
     9001 atcgtatacg acgatctgga atttggtgtt cctgatctag tggaagaggt ctccaaaaaa
     9061 gccacttctg actggtctgc tgtttcctct gaaaagctag ctgaattaga tgctgactac
     9121 atcatccttg tgaacagtga caaagatgct gccatgttca atgaaccaag ctggaagaat
     9181 ctaaaagcag tgacagacaa tcatgtactg caattcggac ctgaatctag ttggctatat
     9241 aacggaccta ttgccagtca ggaaatggtc aatgatatca aagaaaaact aaattgattt
     9301 taactaaaaa aagcatcttc acgattagtc gtgagatgct ttttttctta ttaacaaact
     9361 agacgcatag attttattct tcttttgaag caaccctatt ttttactctt tcccattttt
     9421 gaaaacgatg ggcatataga ttttcttgat ccgttatccc ctcatactcc tctataaggg
     9481 cgaagtctga aaaatcaata ttcccaatat acgtatcgcc ttcaaacgtg ctatcgatca
     9541 atgttcgcca gatccgacca gtgtaaggaa gaagttcttg aaaaatccga ctaccacccg
     9601 aaacatagat atcttctgag atcgtttgac tatacgacaa aagttcatcg atcgaatgca
     9661 ttacttccgc attttctttc gtgcgaaaat ttggttgcgt tgtcaacacg atgatatggc
     9721 gataaggaag tgaaagatcc cccattcctt cataggtctt tcttcccatc actaaaattc
     9781 gatccattgt atgttcgcga aagaaacgca tatcatttgg caatcgccat ggtaacaaac
     9841 catttttgcc gatcagacca tttttatctt gtgcccacat ggaaataaac atattatccg
     9901 ctccttatga ttttagatgt tttgagataa aaaaagtctc aattctttga ccagcaagga
     9961 attgagaact ttaaaaacat tattttgttt cacggtgcaa agtaactttg cgttcgcgtg
    10021 ggcaatattt ttgtttttcc aaacgatcag gattgttacg tttgtttttg cttgttagat
    10081 agttacgttc tttacaagaa gtacactcta aagtgatgtt tacgcgcatg tttttccctc
    10141 ccatttttac aagaatcctc taagatgact ctcagacttg attatcttac catgattttt
    10201 tctagattgc tagtgttttt cacgattttg ttaagtaaaa ctacttgacg ataaatacaa
    10261 cggttttact tctcaaacaa aatctttttc tttttgtata aataccctta cacttgttta
    10321 tatagattat gttttttaat aaagggggat actaatatgc catggaacat gaaggacttt
    10381 cctgcatcaa tgaaaaactt agataaattg acaagaaaaa aagcaatcga catcgcaaat
    10441 gcattattag atgaagggta ccctgataac cgtgcgatcc caattgccat tgatcaagca
    10501 aaaaaatggt atgaagatgc tagtgaatca gaaaaacgaa ctttcgaaaa agaaaaaaat
    10561 ccaagcaaga cggatgaaca cgatacgaat cctcgtgctg gaaaactgct tgattcagat
    10621 gtgatcgtag aatatgagga agaacaatgg atcgtgaaat ctaaaggggc aaaaaaggca
    10681 agcaaccatt ttgatacaaa aaaagaagca atcgaaaaag gaaagcaagt agcacaaaac
    10741 aaagaatcaa ctttgttcat ttataaaaaa gatgggacaa aagaaaaaga aatcgattat
    10801 tgaacatatg taaaggggtt gtgacgaaaa tcgtgtcaca acccccttat gttcgaaaca
    10861 atggtgttcc tctgccaact ccgaaccata agtcataata tctataaaac gggaagagct
    10921 gttttttgaa attgtttctt atcactcagt gttaaacggt aggagaaaac ctctttttta
    10981 ttgatatttg attttcatca aacagtcaca tagtcgattg cttctgcttg cggaatcgtt
    11041 tcatctacaa agtagacttt ataccatgtc aagaaactga agattgtcca gatttttctt
    11101 tgctcttctt tacgtccttc gtgatggtca tctagcaact tcatgatttt ttcttgatca
    11161 aaaaattctt ttgcaaaatc tgcttcgaat aatgctttga cttgttcgta gccgtgttct
    11221 tctttcaacc aagctttgat tggcacaggg aatcctaatt tcacacgatt cgaccattct
    11281 tccggaagat gtttgttcgc agcttgtctg aaaatatctt ttgtgttgtt ttgattcaat
    11341 aaaaacttac ttggtatttg ttgagccaat ttcatgactt cgatatccaa taaaggtact
    11401 cggacttcca aagaacttgc catagaaagt ttatctgcct ttaacaagat atcttttggc
    11461 atccattgat gaagatcgac atattgcatc ttattgactt catctttgat tccttctgtt
    11521 ttctcgtaat gaacagtcat gatttcatcc acagatggtg ctgtttgata ttttggtgtt
    11581 aacacttctg atgcttctga ttcttcgaac actcgagcat gaccgataaa atattctttt
    11641 gcaggagcaa gtgattcata taaatggatc cgtccatgga agtttttcat ttttccgatt
    11701 ttacgtccta aattatagcg tgttcctttt ggtaattttt tcaatccttg agcaatcaca
    11761 cggatgaatt ttgaattcgt atggaatcca tacgtttggt atcccgcaaa cagttcatcc
    11821 gccccttctc cagattggac tacccgcaca ctttgtgaag caagatttgc taaaaagtac
    11881 aaaggtacgc aagaaggatt tgaatcaggt tcatctaagt gatactggat caaagggaat
    11941 gctttgaacg acatgtctcc gtcgatcact gctgctgtat tatctaagtc caatagttcg
    12001 gttagttttc tggcttcaat cgcttcatta taggttttat catcaaaccc gattgagaaa
    12061 gaatgatctg gtttcaatac agaggttaca taactggaat caaccccgct ggataagaaa
    12121 gaaccaactt ctacatcact gaccgtatgt gcttcaatag aagcttgaac ggtttcgtcg
    12181 attttttcga tccattcttg tcgggaatgc gtttcttttg tttcaaaatc tgcatcccaa
    12241 tattgctgaa tgtctatttt tccatcttta tacgtataat aatggccttc aggcaaacga
    12301 tagacatctt tgaagaatgt ttcgccgttt aatggagaat attggaaagt catataaggt
    12361 ttcaacgctt ctttgttcaa ttctttgtta aaatcaggat gaggcagaaa acttttgatt
    12421 tctgaaccaa acataaatgt tccgttcatt tctgcataat aatatggttt gatcccaaag
    12481 tggtctcttg caccgaataa ctcattcttt tcattatccc aaatagcaaa agcaaacatt
    12541 ccgcggatct tttgcaataa ttctgttccc cattcttcgt aaccatgcaa aaggacttct
    12601 gtatctgcat gtgtttgaaa aacgtggcca gctgcaatca attcttctct caaaggctgg
    12661 taattataga tttcgccatt aaaaataatg atctttgtcc gatcttcatt gtaaataggc
    12721 tgagtaccgc cctctaagtc aataatactt aatcttctga atcctaaagc aacatgttta
    12781 tcgatatatt ctcctgaact attaggtcca cggtgaacga ttcgatccat cattgtatta
    12841 ataattgttt ttttgttgtc cttgtcgtta acaaaaccta caataccgca catttaactt
    12901 tcacatccta atataaaatt actgtttatt
//
LOCUS       DAYYHX010000067        12926 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000067 DAYYHX010000000
VERSION     DAYYHX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.067"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            410..979
                     /locus_tag="ACHA6Y_001439"
     CDS             410..979
                     /locus_tag="ACHA6Y_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM8987540.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            966..1826
                     /locus_tag="ACHA6Y_001440"
     CDS             966..1826
                     /locus_tag="ACHA6Y_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8987541.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2016..2720
                     /gene="yycF"
                     /locus_tag="ACHA6Y_001441"
     CDS             2016..2720
                     /gene="yycF"
                     /locus_tag="ACHA6Y_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM8987542.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2725..4560
                     /gene="walK"
                     /locus_tag="ACHA6Y_001442"
     CDS             2725..4560
                     /gene="walK"
                     /locus_tag="ACHA6Y_001442"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM8987543.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4557..5873
                     /gene="yycH"
                     /locus_tag="ACHA6Y_001443"
     CDS             4557..5873
                     /gene="yycH"
                     /locus_tag="ACHA6Y_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM8987544.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5874..6743
                     /locus_tag="ACHA6Y_001444"
     CDS             5874..6743
                     /locus_tag="ACHA6Y_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM8987545.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6798..7607
                     /locus_tag="ACHA6Y_001445"
     CDS             6798..7607
                     /locus_tag="ACHA6Y_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8987546.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7710..8339)
                     /locus_tag="ACHA6Y_001446"
     CDS             complement(7710..8339)
                     /locus_tag="ACHA6Y_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8987547.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8509..9801
                     /locus_tag="ACHA6Y_001447"
     CDS             8509..9801
                     /locus_tag="ACHA6Y_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8987548.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9877..10539)
                     /locus_tag="ACHA6Y_001448"
     CDS             complement(9877..10539)
                     /locus_tag="ACHA6Y_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8987549.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10764..11048
                     /gene="groES"
                     /locus_tag="ACHA6Y_001449"
     CDS             10764..11048
                     /gene="groES"
                     /locus_tag="ACHA6Y_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM8987550.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11099..12724
                     /gene="groL"
                     /locus_tag="ACHA6Y_001450"
     CDS             11099..12724
                     /gene="groL"
                     /locus_tag="ACHA6Y_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM8987551.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4306 a         2223 c         2794 g         3603 t
ORIGIN      
        1 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
       61 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      121 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      181 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      241 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      301 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      361 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      421 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      481 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      541 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      601 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      661 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      721 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      781 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      841 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
      901 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
      961 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1021 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1081 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1141 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1201 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1261 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1321 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1381 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1441 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1501 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1561 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1621 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1681 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1741 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1801 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1861 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     1921 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     1981 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2041 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2101 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2161 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2221 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2281 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2341 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2401 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2461 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2521 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2581 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2641 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2701 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2761 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2821 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     2881 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     2941 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3001 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3061 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3121 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3181 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3241 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3301 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3361 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3421 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3481 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3541 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3601 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3661 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3721 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3781 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3841 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     3901 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     3961 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4021 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4081 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4141 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4201 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4261 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4321 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4381 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4441 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4501 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4561 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4621 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4681 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4741 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4801 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4861 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     4921 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     4981 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5041 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5101 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5161 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5221 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5281 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5341 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5401 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5461 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5521 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5581 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5641 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5701 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5761 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5821 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     5881 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     5941 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6001 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6061 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6121 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6181 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6241 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6301 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6361 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6421 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6481 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6541 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6601 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6661 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6721 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6781 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6841 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     6901 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     6961 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7021 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7081 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7141 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7201 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7261 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7321 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7381 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7441 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7501 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7561 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7621 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7681 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7741 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7801 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7861 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     7921 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     7981 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8041 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8101 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8161 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8221 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8281 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8341 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8401 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8461 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8521 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8581 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8641 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8701 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8761 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8821 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     8881 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     8941 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9001 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9061 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9121 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9181 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9241 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9301 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9361 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9421 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9481 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9541 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9601 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9661 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9721 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9781 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9841 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
     9901 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
     9961 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10021 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10081 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10141 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10201 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10261 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10321 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10381 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10441 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10501 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10561 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10621 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10681 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10741 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10801 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10861 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    10921 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    10981 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11041 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11101 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11161 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11221 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11281 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11341 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11401 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11461 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11521 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11581 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11641 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11701 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11761 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11821 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    11881 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    11941 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12001 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12061 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12121 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12181 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12241 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12301 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12361 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12421 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12481 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12541 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12601 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12661 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12721 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12781 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12841 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    12901 tgtttagagc gtgagacaaa agtgtt
//
LOCUS       DAYYHX010000068        12737 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000068 DAYYHX010000000
VERSION     DAYYHX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.068"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHA6Y_001451"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHA6Y_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8987552.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACHA6Y_001452"
     CDS             complement(322..891)
                     /locus_tag="ACHA6Y_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM8987553.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACHA6Y_001453"
     CDS             1077..1979
                     /locus_tag="ACHA6Y_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8987554.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHA6Y_001454"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHA6Y_001454"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM8987555.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHA6Y_001455"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHA6Y_001455"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM8987556.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHA6Y_001456"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHA6Y_001456"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM8987557.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACHA6Y_001457"
     CDS             8547..8732
                     /locus_tag="ACHA6Y_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM8987558.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACHA6Y_001458"
     CDS             complement(8794..9990)
                     /locus_tag="ACHA6Y_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8987559.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACHA6Y_001459"
     CDS             complement(10160..11596)
                     /locus_tag="ACHA6Y_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8987560.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ACHA6Y_001460"
     CDS             11889..12698
                     /locus_tag="ACHA6Y_001460"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8987561.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4011 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaatt
//
LOCUS       DAYYHX010000069        12687 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000069 DAYYHX010000000
VERSION     DAYYHX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.069"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..962
                     /locus_tag="ACHA6Y_001461"
     CDS             <1..962
                     /locus_tag="ACHA6Y_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFM8987562.1"
                     /translation="KSNNVIIGFLFTKQSGSIKDYKGTPRTVLVQRHDELYLVNRTSG
                     ENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAENLYRNSLGADSLKN
                     ARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFFKEYDYVFVCIGDTM
                     ASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFNCLYTDSNSKNRCSN
                     RASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKVNQNNMTNSTLFSWI
                     GDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            955..1416
                     /locus_tag="ACHA6Y_001462"
     CDS             955..1416
                     /locus_tag="ACHA6Y_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFM8987563.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            1429..2289
                     /locus_tag="ACHA6Y_001463"
     CDS             1429..2289
                     /locus_tag="ACHA6Y_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987564.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            2327..2716
                     /locus_tag="ACHA6Y_001464"
     CDS             2327..2716
                     /locus_tag="ACHA6Y_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8987565.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            2703..5150
                     /locus_tag="ACHA6Y_001465"
     CDS             2703..5150
                     /locus_tag="ACHA6Y_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8987566.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            5155..7263
                     /locus_tag="ACHA6Y_001466"
     CDS             5155..7263
                     /locus_tag="ACHA6Y_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8987567.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            7260..8264
                     /locus_tag="ACHA6Y_001467"
     CDS             7260..8264
                     /locus_tag="ACHA6Y_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8987568.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            8283..9194
                     /locus_tag="ACHA6Y_001468"
     CDS             8283..9194
                     /locus_tag="ACHA6Y_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8987569.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            9381..9668
                     /locus_tag="ACHA6Y_001469"
                     /pseudo
     CDS             9381..9668
                     /locus_tag="ACHA6Y_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9856..10071)
                     /locus_tag="ACHA6Y_001470"
     CDS             complement(9856..10071)
                     /locus_tag="ACHA6Y_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987570.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(10330..10539)
                     /locus_tag="ACHA6Y_001471"
                     /pseudo
     CDS             complement(10330..10539)
                     /locus_tag="ACHA6Y_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(10842..12281)
                     /locus_tag="ACHA6Y_001472"
                     /pseudo
     CDS             complement(10842..12281)
                     /locus_tag="ACHA6Y_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         4170 a         2181 c         2557 g         3779 t
ORIGIN      
        1 aaaagtcaaa taatgtcata attggatttc tttttacaaa acaatctgga tctataaaag
       61 attataaggg tacgcctagg actgtattag ttcagcgtca tgatgaatta tatttggtga
      121 atagaacgag cggagaaaac tatggattgg gtgctaatat agctattgtt ggggtagggt
      181 cagttggcag taaaattgct gaaaatcttg gtccattagg tgtgagaaag atgacactca
      241 tagacaatga ttgttttcaa gctgaaaatt tgtatcgaaa ttcacttggt gcagatagtt
      301 taaaaaatgc ccgatctcag aataattttt ttaaagttga tttaatgcag gaggagctcg
      361 aaaaaaaaat cccatacctt tgtgttgaag tggagtcaat gaacgtttta gacttaatta
      421 gtatgcaacc tggttttttc aaggaatatg actatgtttt tgtttgcata ggagatacaa
      481 tggcaagcct ggctttaaat aattattttt ccaaaagtag tcaaaaagtt ttctattctt
      541 ggctggagcc atatggcatt gggggacaca ctctatttat aaattattct ctaaatcaaa
      601 aaggttgttt caattgtctt tatacagatt caaactctaa aaataggtgt tcaaatagag
      661 cttctctggt aaaagaagga caaatttttg aaaggacttt agcgagttgc cgctcaaaat
      721 ttataccata taatgctatt gcctcgacgg aatgtgcaat taatgcaata aaacttttcc
      781 ataaagtcaa tcaaaataat atgaccaata gtactctttt ttcatggata ggagatggga
      841 gtacttttga gaatttagga tatcaatata gcgacagaaa tacttttctt aatggagtca
      901 ataagtttga agaaagattc ttttctaaat attgcaaagt atgtggggag ttagatgatt
      961 aaattttcgt gtgaccgtat tgtcattgaa gtaagggatg aagttcttaa gatatttgaa
     1021 caatatcggc agggaaagga tgacttgcct gaagctggag gagttcttca tggaaaaaga
     1081 cttaagaatg ggagcataat aattaccgat ataacaacac ctcaaatagg agatatacaa
     1141 aaaagattac tctttaaaaa aaaccgagaa attcatcaac agctttctaa tgagaaatgg
     1201 atagaatcaa ataagttttc tattgcattg ggagaatggc atactcatcc tgaagctgta
     1261 ccaactccat caaatgttga taagaaaagc tggagactga acgtgagcaa gcaacatgat
     1321 gatagagttt atgtttttat tatcgttgga ttttctgatt tgagaatgtg gttactgtca
     1381 aaaaaatcag atattaggca ggctataaaa ctataaagga gtgattaggt gaaatcacaa
     1441 aagaatattt ggtggtgggg gtttttagta ggaaaggtaa tgataccagc ccttctctta
     1501 atttctctac cgatgattac ttttttcttt accaataaaa aaatgagtaa agaagccgtg
     1561 atttttttct ttggagacca aaaagctacc tttattgaaa cgttagtaac atctcttcag
     1621 cttaatttga attacatttt ttcaattatt attgttatat cgttattgaa tttttttaaa
     1681 aaaagaaatt caggaaaggt gtttaactct aatggaaatg tgtactacaa ctatttttat
     1741 tttgtatttt gggtagcagc aactttactt ggttatgata aaattcagat tgcaggaatt
     1801 cctatacaca tgcaatataa gttggttttg agtggaattt tttctgaggt gttgccagat
     1861 atatacgatg atcattacga cagtgatgga acatgtaaag taagcattga aaaagaaaat
     1921 ttcgatgaca tcgatggata tgactctgtg aacttactga ttattgatac ttacgatata
     1981 aaaatgagtg aattatctat ggaaaatcaa acatatccta cgataattgt tagagggaat
     2041 tcaatagatg gagttcgtaa agttaataga tctttaatct tggaaataaa aaaaacaatg
     2101 gatgaaattc aaaaaagtga ctttaaaaaa gtatttgttg cttcaacatc taatccaaaa
     2161 aatagtatca atattattaa ttcatcgttt agatttttcg gtcgatcacg taggtttaaa
     2221 ctttatgttc ttcaaaagga ttatgcaagc aatggaaagt atagtaagaa atatagaatt
     2281 tttatttaag aaggaatttt tccttctttt tattttggag gaaactatga aaaaaataaa
     2341 aagctacacc agtatttggg cagttgaaaa agtcatttat gcgattaatg actttcagct
     2401 gccttttcct gtgaccttta accagatggc ttggtttgtt ttatccttga tgtttgtcat
     2461 tgtatttgcc catgtgcctc cgctatccat gatagagggg gcttttttaa aatacttggg
     2521 cattccgata gcggtcactt ggttcatgtc gcaaaagacc tttgacggta agaaaccatt
     2581 gggattttta cgatccttta tcagctatca cttacggttt aaggtcactt ttgcaggaaa
     2641 gaaagttaaa gaacagaaga aacgatggga cgaaccaatc accttggtaa ggagtgtgaa
     2701 ttatgtaccc gattaagtat attgaaaata atcttgtctt taaccaagaa ggcgagtgtt
     2761 ttgcttacta tgaattagtg ccttacaatt actccttttt gtcacctgaa cagaagtatc
     2821 aagtacatga taactttcgc caattaattg cgcaaaatcg tgaaggtaag atccatgcct
     2881 tacaaattgc cacagaaagt agtattcgtg ccacacaaga acgttcgaaa aaggaaatta
     2941 ccggtagact caaagaagta gcaaagcaac gaattgattt acagacagat gcattagtat
     3001 ccatgattgg cgatagtcag attgattatc gcttctttat tggctttaaa ctgatttcga
     3061 cagatgaaga ggtcaatcta aagaatctca ggaaatcttt cttttctgga cttcaagaat
     3121 ttgtctatgg agtgaatcat catttgatgg gtgattttgt ttccttatcc aatgaagaaa
     3181 ttcgtcgcta taccaagctt gaaaaactaa tggaaagtaa attggctcga agatttaaag
     3241 tcaaacgagt aacgccgagt gacttgacgt atttaattga acatatctat ggtgaaaaag
     3301 gcacaccgtt tgaagaatat gaattccagc taccaaagaa aaagctgaaa tcagaaacgc
     3361 tggtgaaacg ttacgactta ttacgtccca ctcgttgttt gattgaagaa aagcctcggt
     3421 acttacggat ggaacatgaa aatcatgagt cttatgtagc gtacttaaca attaatacca
     3481 ttgtagggga aatggagttt ccttcctcgg aacttttcta ttaccagcaa caacaattta
     3541 cgtttccgat tgatacttca atgaatgtgg aaattgtgac gaataaaaag gccctagcta
     3601 ccgttcgtaa taagaaaaag gaattaaaag atttagacaa ccatgcctat caatctgata
     3661 atgaaacgaa ctccaatgta ttagatgctt tggattccgt ggatgaattg gaaaccacct
     3721 tggatcagtc gaaagaatcc atgtataaac taagctatgt ggttcgagtg agtgctaaat
     3781 cagtggatga attaaaacgc cgctgtgatg aagtgctcga tttttatgac gataccaatg
     3841 taaaactagt ccggcccttt ggcgatatga tgggtctaca caaagaattc ttaccctcaa
     3901 gtaagcgata catgaatgac tatattcaat atgttacgtc tgactttttg gcgggacttg
     3961 gttttggggc tactcaaatg ctaggagaat tagaaggcat ttactttggc tataacgtag
     4021 atacggggcg caatgtttac ctgaaaccgg cattggcatc acaaggggtc aaaggctcag
     4081 taaccaatgc tttggcatct gctttccttg gttcgttagg tggggggaaa tcttttagca
     4141 acaatctctt agtctattat gccgtccttt ttggtgggca agctgtgatt gtcgatccaa
     4201 aaggagaaag aggcggttgg aaagaaacgt tgccagaaat tgcggatgaa attaatattc
     4261 ttaacttgac cagtaagcca gaaaatcaag gcttacttga tccctatgtg attatgaaga
     4321 aaaagaaaga ctcagaaagt ttggcgattg atatcctgac ctttttaaca gggatttcca
     4381 gtcgggatgg ggagaagttc cctgtgttac gcaaagccat tcgaacggtt acccaaagtg
     4441 atcagcgagg attattacaa gtaatcgttg aattgcgtaa agaggactca ccagtcgcgg
     4501 aaaatatcgc ggatcacatc gagtccttta ccgattatga ttttgcccat ttgcttttct
     4561 cagatggttc gattacccag tcgattagtt tagataagca gttaaatatc attcaagtag
     4621 cggatttggt tttaccagat gcagaaacca cttttgaaga atataccacc atggaattgc
     4681 tgagtgtttc catgctaatt gttatttcca cttttgcctt ggactttatc catagcaacc
     4741 gagaaatttt caagattgtg gacctggatg aggcttggag ttttcttcaa gtagctcaag
     4801 gaaaaacgtt atcgaataaa ttagtgcgtg ctggtcgggc catgaatgcc ggtgtttact
     4861 tcgtcacgca aaacagtgat gacttgttgg atgaacgttt gaagaataat attgggctaa
     4921 agtttgcatt tcgcagtacc gatattcatg aaatcaagaa aaccttggaa ttctttggat
     4981 tagataaaga agatgaaagt aatcaaaaat gcatgcgaga tttggaaaat gggcaatgct
     5041 tgatgcagga tttgtatggt cgagtaggtg tggtccaagt tcatccagtc tttgaagaac
     5101 ttttccatgc ttttgatacc cgtccgccag ttcaagagag aatggagtga ggagatgaaa
     5161 aagaaacttt ttcgtatctt tggaattgtt ttaatcagtt tggtcactct gataatactt
     5221 ttgagcttag ttggaacggt agctgaggca acaggtctgg ttgatgatac agttggagcc
     5281 ggaaatcttt attcccaata ttcacttggt aactatcagt tggatttctt tgtggatagt
     5341 tcgtgggatt ggctgccttg gaattggggt gatggcctag ggaagagcgt aatgtatgga
     5401 ctttatgcca ttactaattt tatttggaca gttagtctct acttatcaaa tgccacgggc
     5461 tacgtggttc aagaagccta taagctggat ttcatttcag atacggctga aagtattggg
     5521 aagaatatcc aaaccttagc cggcattacc gaaaatggac tccagacctc gggcttttac
     5581 tttgggtttc tattattgat gattttagct ctaggagttt atgtggcgta tacaggatta
     5641 ttaaaacgag aaactaccaa agcagtccgg gctgtcatta attttgtgat gatttttcta
     5701 ctttcgggtt cgttcattgc gtatgcgccc acctatatca caaaaatcaa tgattttagc
     5761 tcagatgtca gcgaagcggc tcttagcctt ggaaccgaga ttgtcgtgcc gaattcagaa
     5821 agccaaggaa aggacagtgt ggatttaatc cgagatagct tgttttccat tcaagtccaa
     5881 caaccgtggt tactcttgca atttgatgat tccaatatag aagaagttgg cgaagatcgt
     5941 gttaacaaaa tcctgtctgt aagtccagat gaaaataaag gcaaggatcg ggaagaagcg
     6001 gtcaaagcgg agattgaaga taatgacaat gcgaatctca gtattaccaa gaccatgaac
     6061 cgcttaggga ccgtggtctt tttggtgttg ttcaatattg gcatatcctt ctttgtcttt
     6121 ctcctaacag ggattatgtt gttctcgcaa attctcttta ttatctttgc gatgtttttg
     6181 ccgattagtt tcttattgtc catgttgccg acttatgaga gtttgggcaa gaaggcgatt
     6241 attcgcttgt tcaatacgat tatgatgcga gcaggagtta ctttggtgat tacgactgca
     6301 tttagcatct ccacgatgtt tttcaatatt tcggcaacct atccattttt catggttgct
     6361 tttctgcaaa ttgtgacctt tgccggtatc tatttcaaat tgggcgacat tatgaatatg
     6421 ttcaatctcc agagtaatga tagccaatca atgggaagac gggtcatgcg aaaaccgcaa
     6481 atgttaatga atcgaacatt gcggcaactc aatcgaaatg tgggacggac tttggctttt
     6541 ggtggtggtg cagcagttgg aaataagttg gcaaaggaac aatcaaaatc caaatttaag
     6601 ccatccggtt cttctctacg aaagaattca cgattaccga acgatcacga gataccatct
     6661 gacttgaata aggaaaagcc aattccaaat aataagaaac aatcccgaat gaatttggcc
     6721 gggagaaaag tcggcaaggt tctggatacc caagctttgg tgaaagataa gaccaaacaa
     6781 gtaaaggatg gagtacggaa tacgccaacc aacttgaaat acaacttcca taaaggaatt
     6841 gaaaaaacga aaaaagcacc ggaagagttt aagcgaggac tggtccaaga aaaagcaaat
     6901 cgaggagagt tgcgagaaaa acaacaacaa cgtagagacg aaaaaatagc tgaaaagcgc
     6961 aaagtattga acgaaatagg gaatcctcat gggaaaagaa gggaaaatgt tccgataaaa
     7021 gcaaaggtcc aatctcaaaa ggatagagca ccaaagcgga ttgtgagagc aaattcgaat
     7081 cccgaaatca aacgaaaatt cgctagccaa gaaatcattt caaaaggaat aaaccaatcc
     7141 tcaagtaaga aaaattcttt tcaacaagtg aaacggcggt ctactctgtc caaacgaacc
     7201 aatcgaaaag tacaaaaaag tttgaaccct catcccaaac caaggtcagg tgagaaaaaa
     7261 tgaacgtaaa gaaattaagt gccatttcgt taactttcct aacaatctct tttatgggta
     7321 tcttgatgtg tgtgggctta ttgtttggtg aagacagtga aggaagtagt agttcaggtg
     7381 ctcctcctgg ggggagtagt gtctcagaag aggttttgaa acaccgtgca atggtggaaa
     7441 aatatgccaa agaatcggat ataacagagt atgttccgat tcttttagcg attattcaag
     7501 tggaatctgg tggcacaatg gaagatgtga tgcaaagttc tgagtcagcc ggtttaccac
     7561 cgaattcatt aagtaccgag gcatccatca aacaaggatg tctctatttt gcgtctttgg
     7621 ttaagcgttc caaagaatta ggttgtgatc aagatagtat cattcaggcc tataactttg
     7681 gcggtggtta tttggactat gtggcaaaaa acgggaagaa gcatagtttt gctttagctg
     7741 aatctttttc aaaggaaaaa tcttgcggtc aaaaaatcga ttaccctaat cccatcgcga
     7801 ataaggaaaa tggtggatgg cgctataact atggcaatat gttttattgc ttattggtga
     7861 agcaatacct aactacaaca cagtttgacg ataaaacagt ccaagggatt tttgacgagg
     7921 cgtttaagta tgaaggaacg gcttatgtat ttggtgggtc aagacccgaa acaggatttg
     7981 attgtagtgg cttgactcaa tggtgttatg ccaaagttgg tctgaaactt cctcgggtgg
     8041 ctcaagatca gtatgatgcc atgacccata ttgacctaaa agatgccaaa ccaggggatt
     8101 tggttttctt tcattcgact tatgatgccg gtacttatgt gacccatgtc ggtatttatg
     8161 caggggagaa tcgcatgttt catgcaggag atccagtagg ctggacgaat ctaacagaaa
     8221 gctattggca gcagcatctt atcggtgctg gacggtacaa ataagagaaa agaggaaaaa
     8281 gaatgaagat aaaaattgaa cggaatcaaa aggaaaagag cctaaaaaag aaaaaagaac
     8341 gagtcgtatc cgtgggtaag catcgaaaaa tggtgcttgc cctttggctt ttattatctt
     8401 gtagtcttac ttttggcgtt tataaaaatt tcaccgccat tgatcaacat acaactcatg
     8461 aaaaagtggt tattaaagag aaagtagtga atacttctgg tattgaaaac tttacgaagg
     8521 atttttcgaa agaatatttt tcgtggaaaa ataacaaaga agcaattgaa aagagaatga
     8581 acaacctcga acagtacctt acagaagaag gtttcgcttt aagtcaagat atggttcgag
     8641 cagatattcc cactaattcc gaagttcagt cagtaaaagt cttagatgtt gaaaagagct
     8701 cggaggaatt tgtcgtttca tttttagtgg agcaaaagat tacagaggga aaaaaggcac
     8761 aatcgatttc ttctgcatac cgagtgacga tctttgaaga tgaaaacagg aattatatag
     8821 taacgagttt gccaactatg attgcaaaac ctgatagggc aaaatacaag acaaaacaag
     8881 tagaaaatga ttcgaaaatt gatgccaaaa ctacagaaga aatcgctgag tttttggaaa
     8941 cgttctttaa attatatcca tcagcttctg aaaaagaatt ggagtattat gttgaaaatg
     9001 atgcgatgcg accaatcaat attgacttaa agttttcgaa gttagaggat ccagtttacc
     9061 tacaaaacgg aaaaaaaatt ctagtgagta caaagataaa ataccttgat aatgtatcaa
     9121 aaaccactag ctactttcag tacaaattaa tattacaaaa aaatggaaac tggaaaatca
     9181 ttgatgttaa ataataatga ctcaatctaa atttatgaga agttataagt agtttaactt
     9241 atttcaggca gaagtcgccc atcctctata ggtgggtgat gaatgccgtt cggtaactcg
     9301 aaaaccgaca attaagacat agtgcctttt tattttaatc catgtcataa tcagtctata
     9361 taagaaatgg ttgatatacc atggaattag atacaaataa tcattcatta tttcttcttt
     9421 gttaccacct tattttagta gtgaaacatt gtaaacatct cattgatgat gtaatgttag
     9481 cttatgcaaa aaactatctt tgaaagaata tcagaatcac atcacattac cttagttgag
     9541 tgaaatcgtg ataaagacta ctttcatatt atgctcaaag cacaacctaa aaccgaatta
     9601 acaaaattca gcaatgccta agctcatgtc tgataaaatg agactttcta agggtcaaaa
     9661 atttcctttg aatacgatca ttagcttaga taaaagtaaa aaccttagaa ctacggggat
     9721 agcttggtta ataagagata tcgctgttaa taaagacata agcttgcaag tatgctctat
     9781 tcccaagaat ctcccacttc aagcactaag gatcgttaaa ttcagctaag tggcaagtag
     9841 ttcactagac gataattatc tccatatttc aatctcagct tttccatttt tcatagcaaa
     9901 ttccgttatt tttatagcat ctaaaagatt cagcttttcc actgatcttt caccactttt
     9961 cagctttttt attgtgctta aattgatacc agtacctttt gatattgaat tggaagtaaa
    10021 atcattaagg gtttttttta ttaaattcat atccgcaatt ccagcgttca taagaatgaa
    10081 gattctcctc tcatagacta ctttgaaaat ggtactacag tggctgttta tatatttcag
    10141 taggttactg tagtggtcat tttattataa ttagaaaagg aacctaaatc aaaaatgcta
    10201 aagaaaattt tcatagtatt aaacctatca tgactaaagc cacggaattc ttgataaaag
    10261 agtatacttg caagcctatg tctttactaa cagcgatatc tcttattaac caatctatcc
    10321 ccgttgttcc taaggttctt gatgttttct aaggcaatgc ttgtatacat agcccttgat
    10381 caaaatattg atactaacgt tgacgtttcg atcatgatgt gtacggcaaa taggacaagt
    10441 ccattctcga attgtgaatg acttcttacc atctgtgtgt ccacattctg aaccaatttg
    10501 actagacgga aatcatttat ctatcttgat gatttttttg acagaagtat cgaaaatctt
    10561 tacataatct gggtgttgca ttcttatgca gatgagagcg ttagctccgt attcataacc
    10621 agttgcctct aaaacgtagt ccatcggtgg atacctagct tgcggttgcc agtggctttc
    10681 gcagtcttct tgtaatcaaa tcgagcatct aattgagtac tagtattttg aagataatac
    10741 ttgagttttt cggttttcag aagttcactt catcttccat catccatgga aatcccactc
    10801 acccatacgc aggcctccaa aaattcttca acaatggttt ttcagacatt tttctgaata
    10861 cctccttgtt ttttgatacg ccagatagtc aggaaaaagg caacaaaaca caaaacaaag
    10921 ctgcctaaaa aggtaatctt cattccatat aaaaagacat ccggccgatc tgcgatatag
    10981 ttagttactc gttggcccat cttagcagac atcgcttgat ataagaccgt cgtagaaagc
    11041 gaaatcccga tgaccatccc caaatttcgg gcaaaggaat tcatggagcc tgccagtccc
    11101 aaattttcga tagtgacaga actcataacc atcgtattat ttggggactg gaacaaggca
    11161 ttgccggcac ccatgatggc agtagcgaca atgtacagcc acaaaggaga gtccactgac
    11221 aaaaacatat acatgacttg ggttaccgac aataaaccca agcctactaa tacaaaaatc
    11281 tccgggccaa cccggtcggt caggtatccg cttaaaggcg ccccgacgac catcatcaag
    11341 ggaaagacca tcatcaacag tccggcatag ctaggactca acccgcgagc attttgcaga
    11401 taaaaaggaa tcactacatt aacaaagaag ttggaagaaa aaatcaacaa ggctgtaatc
    11461 aagctcatgg taaaaatctt gttcttaaaa ataccgaaag aaatcaatgg agaggctgct
    11521 ttcttttcaa cttttaaaaa gctgacaaag gcaatcactg aaattacgaa cagtacaagg
    11581 gggactggat ggaaaaagcc actttcttgc cccaaaaaga ttgttccaaa aaggctgata
    11641 atcgtagccg caatggcagc aaagccccct ccatcgattg tatggccgct catggtgata
    11701 tcttttggta agaatttttc agcaatcaac atcgttacga ttcccaccgg tacattgatc
    11761 caaaaaatgt acgcccaaga aaattgggac aaaatcaagc cgccaagacc tggtccagca
    11821 atcgagccta atgaaacaag cgcaccgatt gctcccaatg cccgaccacg ttcatgtatg
    11881 ggaaagactt ccgtgatgat tccggcattt gtagccatag tcatactagc accaatggct
    11941 tgtaccaccc gtgcgaacaa ttaaatacct agcgaatgat tgaacccaca caacagcgag
    12001 cccaagacaa agatcagcgt accaatccga aatactttga ttttcccaca gctatcgcca
    12061 attttaccaa acagcaacaa acaagaacag acaatcatca gataaacgga cactacccat
    12121 tccgcttgat tcattggtat gcccatatca tctgagatgg taggtagtgc aatattgaca
    12181 atactgccat ccaatgtgga catgaaggta aacatagata tagcaatcaa aatccaccag
    12241 cgattttttt ggacttttgc atcttcttga taactatgca taataggatt tcctttctaa
    12301 tgatcgtgta gctaagaaca atataaccta tgtctactgc ccaacaactt ctcgcgactc
    12361 tagccatagg aagcttgagt aatttcgatt tgttattctc atgtaaccat gtaagtttga
    12421 gatgctttat ttgaacttac tctatagtaa ctagtgataa ttcgctttac tttcttctag
    12481 caatttctaa aagtcgactt cattgttctt cagattaata gtgtgagcta tctcgtgaga
    12541 cgtaagaaaa tttccattct actttttccc aattttttca tatttatggt gttgcaccta
    12601 tttgttttcc aatttgcttt tcaaatacat tgctatgata ttcaattcca taggattata
    12661 ctaaatgtca atagtatgaa taatata
//
LOCUS       DAYYHX010000070        12662 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000070 DAYYHX010000000
VERSION     DAYYHX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.070"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..415
                     /locus_tag="ACHA6Y_001473"
     CDS             158..415
                     /locus_tag="ACHA6Y_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987571.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            528..1139
                     /locus_tag="ACHA6Y_001474"
     CDS             528..1139
                     /locus_tag="ACHA6Y_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987572.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            1318..1875
                     /locus_tag="ACHA6Y_001475"
     CDS             1318..1875
                     /locus_tag="ACHA6Y_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987573.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(2574..3095)
                     /locus_tag="ACHA6Y_001476"
     CDS             complement(2574..3095)
                     /locus_tag="ACHA6Y_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM8987574.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            3294..6986
                     /gene="nifJ"
                     /locus_tag="ACHA6Y_001477"
     CDS             3294..6986
                     /gene="nifJ"
                     /locus_tag="ACHA6Y_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM8987575.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            7175..8062
                     /gene="cdaA"
                     /locus_tag="ACHA6Y_001478"
     CDS             7175..8062
                     /gene="cdaA"
                     /locus_tag="ACHA6Y_001478"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM8987576.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            8059..9228
                     /locus_tag="ACHA6Y_001479"
     CDS             8059..9228
                     /locus_tag="ACHA6Y_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM8987577.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            9252..10607
                     /gene="glmM"
                     /locus_tag="ACHA6Y_001480"
     CDS             9252..10607
                     /gene="glmM"
                     /locus_tag="ACHA6Y_001480"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM8987578.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(10679..12484)
                     /gene="glmS"
                     /locus_tag="ACHA6Y_001481"
     CDS             complement(10679..12484)
                     /gene="glmS"
                     /locus_tag="ACHA6Y_001481"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM8987579.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT         4056 a         2267 c         2780 g         3559 t
ORIGIN      
        1 tttaaaaggt aacgtgttgt gaccgaatta aatgaattaa tgcctatttt ttatattcct
       61 tagtggacaa aaatggagac agtgaagtta ggagcattgt atcctttgtt gcgtagttca
      121 aaaaagtaag cgatttctat aatggtcagg gtaaacggtg agggctgtcc acgtaaatcc
      181 tactattgaa atcacaatga tcggacattc aaaaggtggt gctgaagcag caaattcagt
      241 agctataaat acgaatgcga ttatatttaa tccagcgaca gtaaatttat ctgcttatga
      301 attagactca aacacatata cagcaaacat ggtagcttat gttgtcgagg gagatgcatt
      361 aaatgttgtt tttggaggag tatacaccta ttggagaatt aatcatgtta gatagacaat
      421 atgatgcacc ttagtggaga atgatatctc gcataggtaa ctcagtaaaa aatcaaggta
      481 tggaagcaat tatctctgca ttggaagatc gatatagata ggcggatatg aatagtaata
      541 gggggaataa aatggaatac aatggatcaa tgagaattgt acaaattata ttttggatta
      601 catcgggaat tgtgatcata ggaggagttt ttttgatgtt gccaagctta atatttcctt
      661 tctttgcatt aatctcacct aaaatacccg aaccggaaat cacctatgga gaatttccat
      721 ttaggattga atacgaacta gatggacaga ttaacataat tgaagatact gtcatagctg
      781 agtttaacgg gtgtgagttc agcgcaggtt ctatgaaaag agaacgaaga tggagaagta
      841 gattagctag tggtagagaa gatctacctt ttggggacga tctaggaatc tattttagtc
      901 gaggaagtgc gcaatattat atgggggaaa atgtacaatc aatgtcatta aaaccacata
      961 tagcccttag aaatctcgag gaaggtttta aaagagaggt agactttata ttaggagaaa
     1021 gtggatacat aagaactgta cttaatttcg gagaagcgca agaagttttg acacaatacg
     1081 gaattacctt aatcaactgg gaaatatctg aaccgattgt taacaatttt ggtgattaga
     1141 aaacaattac gataaataga aacaagccaa atcatcagca agtaaaagct gatgatttgg
     1201 cctttcttat acgagtagta ggaattcata gcgaatatta ttacgaccag tagatatttt
     1261 gatattaaag gagtattacg aactaatatg tgagaatgta gaggtgatgt gcgaataatg
     1321 agaaatttat caaatagaaa caaaatatta atcattattg ttgttatagc tgtgtttcat
     1381 ctgggaacga atgcggtttt aagtagaata atattgggtc ctaagccacc aagaccagaa
     1441 attacccgag gggagtttga ttttagatta gaatatgaag tagatgggga aaggattgtg
     1501 atagaagata caattgttgc attatttgat ggattttctg cagatgcagg tagcatggca
     1561 tggtatcgca cgtggcgtct acatttagca agtgatagac gatcacgaaa tatcttatta
     1621 gatgaattag aggatggtcg aagaatatat tatgtcccag aaagtgctaa ctattctatg
     1681 ggagatgtac agaaaaaaag agagcctaat cctaattggt atccatttaa tggcgtaaca
     1741 atagaatatc cacgcaataa aacacctgaa ataggtgcaa aatttataag tggtttagaa
     1801 gatctgtacg ataggtttgg aatccgtctt attagttggg aacacgatcc accaatagaa
     1861 aatcgctttg aataatcgaa atctactaat ccatttcatc agccagtgaa tgttgtaatg
     1921 gaacaatttg gtttagtgtt caaaaccatt gaaatactag gctgtgatca gcaaaaatga
     1981 atctgaataa aaaagagagc aactattagc tgagaggagt taatagttgt tagctttata
     2041 cgaatcaaag tggcaaatac agatgcgtat gcatttgtgt ttgccatctt gcgtttggta
     2101 gacgagatag gtataagcta aagaaacaac ttgtttaaaa taaacgacct agctcaacaa
     2161 atgaccatct aatcaactaa cagcatacat aaaagcaact atttttgaag tagaattgaa
     2221 atcgggataa cttaaaaagt gattgggata ctagtgagaa tcaccgtaca taaatattac
     2281 tcgttaaggg aaattgagaa attttgtatg aaaatttaag aattagttag tatgcgcaag
     2341 tcaaaaatat gaataaatta tttggtggag ataatttgct tagcttgtca taagaaatga
     2401 gataatctgg cagatgcaag cttttggaca ccatttattc ggaataagga ggctgggaca
     2461 gaagcgttta actccaagaa ataagaagga gttgcttctg cttccgccgt ttatacgtgt
     2521 ttagagcgtg agacaaaagt gttctttact tttgtcccac gctccttatt tttttatttt
     2581 gtttggatca attcttttaa ttcctctatt gaaatagatt catcaaagat ttctttaccc
     2641 aagatgacag ttggaacaaa tcgaatatgt gcttgctcag cttcgttgat gacagcttgg
     2701 ctcagctgtt cgtctttttg ttctgtcagc tttagtttat ctactgcaaa atccgctact
     2761 tcttggagag aaagatgttt ccactcgtct tgagtatcga atatttgttt gatctctttg
     2821 atcgcttttt ggccatcgct gattgtgaga taccggtgca tcacgttccc tcgctgtaag
     2881 ctttcttttt ctttatcgaa caatttgatc actcgctgca attgcccgct ttggactgct
     2941 tcttctaata gctcataaga ttcttcaaac cattgtcggc aatacggaca agctaagttg
     3001 atgaattcga ccattttttt aggagcattc gatgaaccat aaaggattcc tttttgtgta
     3061 tttgttttcg ttgcatcaat cactgatata tccatgttaa aacacctctt tcgcttatag
     3121 tttatacatg tctgacaaaa aaaggcaacg atttagactg tttgtctaaa aattagatga
     3181 ccaattcaaa tagatacatg taaaatattt ttataaataa aagaaaaaaa tggcatgttt
     3241 tacgttataa tggaacagat aataagagag aatatgaaca aggatgatga atcatgcgaa
     3301 aaatgaaaac aatggatggc aatacggcag ccgcatatat ttcttatgca tttaccgaga
     3361 ttgctgcaat atatccaata acacccagtt cgaccatggc tgaattagta gatcaatggt
     3421 cagcagcagg aaaagaaaac gtattcggtc aaccagtaaa agtagtagaa atgcaatccg
     3481 aagcaggagc agctggtgtg gtccatggat cattgaaaac tggtgcactg acaactacat
     3541 ataccgcttc acaagggctg cttttgatga taccgaatat gtacaagatt gctggggaac
     3601 tactgccaag tgttttccac gttgctagtc gggcagtgac gacaaatgcg ttgaatattt
     3661 tcggagatca cggagacgtg atggctgcaa gacaaacagg atttgctatg ttagctgaaa
     3721 gcagtgtaca agaagtgatg gatttagcac ctgttgccca tcttgcttct attgaaggag
     3781 aagtcccttt tttgaatttc tttgatggat tccgtacgag tcacgagatt caaaaaatcg
     3841 aagttttgga atatgaagaa ttagctcctc tgatcaatca ggagaaatta gcttcatttc
     3901 gtaaacgagg gatgaatccc aatcatccga ctgttagtgg tacgaaccag aatccagaca
     3961 tccattttca acaacgggaa acgatcaacc cctattatga taaattacct gaaatcgttc
     4021 aaaaatatat gaaagagatc aaccgattac gaggaacttc ttatgacttg gtgacttatt
     4081 acggagcaga cgatgcggaa gaagtcattg tttctatggg atcagttgca cagacgattg
     4141 aacaaacgat tgattacttg aatgcaaacg gacggaaaac aggttttttg aatatccatc
     4201 tttatcgtcc ttttccagtg gaaacattcg tgcaaaagtt gcctcataca gtaaaaacaa
     4261 ttgctgttct tgatcgaagt aaagaaccag gggcaggtgg agaacctctc ttattagacg
     4321 ttcaaagtgc gttatatgat tctgaacttc gcccggctgt tattggtggt cgctacggat
     4381 tggggtctaa agatgtgact cccgaccaaa tctcagcagt ttttgatgaa ttgaaaaaag
     4441 acccaagcat acgcaaaaaa cggtttacca tagggatcgt ggatgatgtg acttatcaat
     4501 ctttggaacc aaaggaaagt ctggatttga cagaaccgca aacattccag gccaagtttt
     4561 ggggatttgg atctgacgga acagttggag caaataaatc agctatcaag attattggag
     4621 atcacaccga taaatatgcg caaggctatt tttattatga ttcgaaaaaa tctggcggtc
     4681 tgactgtctc tcatcttcgt tttggagata cgccgatacg ttcagcgtat cttgtcgagc
     4741 atgcggacct tgttgcctgt cacacaccag cttacctgca tagctatgac ttggtcaaag
     4801 gattgaagcc aggtggcatt tttttattga atactctttg gagcgacgaa caattagaaa
     4861 cgcatctgcc cttaaaatta aaacgttact tggctgaaaa taacattaga ttctatacga
     4921 tcaatgccat gagattggcc caagaagtcg gtttgggacg ccggatcaat acggcaatgg
     4981 aaacggcttt ttttaaatta gctgacatca ttccttttga tgaggtgctt ccgctgctaa
     5041 aagaagaagc cctgaaaagc tatggacata aatcaatggc tatcgtagaa aaaaacatac
     5101 aagcaatcga tcggacgacc gaactgcttc atcaagtcgc gatcccagac cattggagaa
     5161 cgttggagat gcctgaaaaa atcaataaaa ggaacactgg ttatatacaa gaaatacttg
     5221 aaccagtgaa tgcacaggaa ggaaatcaac tctctgttgg tacacttgta gcaaatggaa
     5281 tgacaactgg cgaaatgcct ttaggaacgg cttctatgga aaagcggggc gtcgctttag
     5341 aagtacctga gtggattagt gatcgctgta cgatgtgtaa tgaatgtgcc tttgtttgtc
     5401 ctcatgctgc catccgtcca tttttagcag atgaagagga aatggaagaa gcgccagaag
     5461 gatttatcgt gagagaaatg cgaggcgcag acggcgtaaa ataccgtatc caagtttcag
     5521 tggaagactg tacaggttgt ggtctgtgtg tagaggcttg tcccgcaaaa gaaaaagctt
     5581 tagtgatgaa gccttatgaa gaacagaaag aacaagctgt gaattgggct tttgcaatga
     5641 cattaagaca aaaagcaaat ccagtcaaag gaaagaatac tgtcttgtct acacaattca
     5701 atcaaccgct tcttgaattc tcgggcgctt gctcaggctg tggcgaaaca ccttatgtaa
     5761 aattattgac acaaatgttc ggtgatcgaa tgatgatcgc gaatgcgaca ggctgttctt
     5821 ctatttgggg cggtgcttct cctgcttctc cttatacgac caatgaatgt ggtcaagggc
     5881 ctgcatggtc aaattcttta ttggaagata atgctgaatt tggttatggt atgcttctag
     5941 cggcacagac tcgtcgcgag gctttggcaa gttcaatcaa agaagtattg ccaaaagtat
     6001 cagaagaact acgagaattg atggaagact ggttagtaca catgtttgag agtgaaggaa
     6061 cgcagcaaag agcagcaaaa ctgcaagctg cgatcgaaag cgaacgatca attttccctg
     6121 aactggagga tatatacaag agaaaggatc tattcgtaaa aaacagccaa tggatgattg
     6181 gtggagacgg atgggcttac gacattggtt ttggcgggat cgatcatgta cttgccagcg
     6241 gagcagacgt gaatatcctt gttttggata acgaagtcta ttccaatact ggcggtcaaa
     6301 cctcaaaagc aacaccagct tcggcaatcg ctaaattttc ggctagtgga aagtatgctt
     6361 caaagaaaga tctaggaatg atggcgatga catatggaaa tgtttatgtg gcgcaaattg
     6421 cttcaggtgc caatcaaatg caaacgatca aggcatttga agaagcagaa aagtatcctg
     6481 gtccttctct gattattgcc tatacgcctt gtatcacaca tggcttgctt ggcggaatga
     6541 aagaatcgct taaagaagca aaagaagctg tttcttctgg ttattggtca ttatatcgat
     6601 ataaccctac aatggtcgaa aaaaacaaga acccaatgac tttggactac aaaaaacctg
     6661 atttctctgc tatgcctgat ttcatgagga aacaagttcg cttttcctcg ctagataacg
     6721 caaatccaaa tcttgcaaaa gctttatttg aaaagactgt ccacgacgct aaaacacgtt
     6781 tttacaacta tgctgcactg acgggagaat tagaaaaaat caagaaacga cttgaaccag
     6841 aggaatctat cgatgtaccc gttaagacta gaaaagaaaa aacgacggac cttgaagcag
     6901 aagaaagaag agctgctaga agagctgcca gagcagaacg acgtaaaaaa atggaacaag
     6961 ctacaaaaga aaaaaaggaa aaataacaga aaaaatataa tgaataattc tctgaagttt
     7021 aaaaatattt attgcagaga aaaataaaac gcatacttgc aattttcttg aaaattatgc
     7081 gtttttctca agaaaaatac gagcatatca gcctctaaaa ttaaaacata tgatatacta
     7141 gagaggaaat ctcaaaagag aagagaggct ttttatgtca tttcaaatcg gggaactttt
     7201 taatttggac tattggcagc aaatgttttc cggtgacttg ttctctctga attttttggt
     7261 gaacatactt gatattctgg ttgtctggta tcttgtatac aaactgatcc aactgctgcg
     7321 aggcacaaaa gcgattcagc ttctgaaagg tgtagctatt tttattgtta tccgaatcgt
     7381 agcggaaatc attggtctgc acaccttgtc ttggctgatg aaccaagtca ttacgtacgg
     7441 ggtgatagct gctatcgtga tttttcagcc ggaaatccgg agaggactgg aacatcttgg
     7501 acgcagttcg cttttcaaac aatcaaaaag cgaacagcag gaagatgaga agatgatctt
     7561 gtcttttgac aaagccatcc aatatatgtc caagcgaaaa atcggtgcgt tgatcactat
     7621 cgaacgtcat actggattag atgagtatat tgagacaggg attgctttag atgcagatat
     7681 cactggggag ctgctgatca atatctttat tcctaataca ccgctgcatg acggtgcagt
     7741 tattgtaaaa gaaggaaaga tcgcagtagc aagcgcctat cttcctctat cagaaagtat
     7801 gttgatccct aaagaattcg ggactcgaca ccgggcagct gtcggtataa gcgaagtcag
     7861 tgacgcgatt accatcgttg tctctgaaga aaccggggac gtcagcatca cgttagataa
     7921 tgaactgatg gctggcttat cccagcaaga atatttggca atcttgcgta gagaactaat
     7981 tgctgaagag aaaacaaaaa acaagaaaaa cgtgattcaa tctttcctag acggtatgac
     8041 taagggaggg gatcgcaaat gatctcaaaa gagaaacgga caagcattct ttacagtttt
     8101 attgcattgc tgttcagtat tgcgctgtac ttcaatgcaa atggccagtc cgttccaaat
     8161 acgctttctg gaaatgaagc gtataatcaa acagttacgg gggtgccgat caagatttct
     8221 tatgattcaa agcgctacta tatccatgga tatgaaaatg cagtgactgt caaactttcc
     8281 agtgcaaacc gagttcagtt gaatgcagag gcaaacgaag atacacggat gttccgcgtc
     8341 acagcagata tcactaagct gggcgaagga acacatgaag ttccgctaaa aattcaaaat
     8401 ttgagcagtg ctgtcacagc aaagatcgaa ccatcgacta ttacagtaac ggttgagaaa
     8461 aaggtaataa aagatttcga tgtggaaacg ttgattcctt caacgttcac accatctgga
     8521 tatgaacttg atgatacttc cgttagtcct aaaaaagtct ctatcactac aggtgacaaa
     8581 acattggcag aaatcaaacg agtagtagct gatgtggacg cttcgatggt cacagatgat
     8641 gggattaata gtgaggtccc aatacaggcg ctgaatgcag caggggaagt attaagtatc
     8701 gtctctgatc cagtgcaagt aacagtgaga gctgattcga tcaagccaag caagtcggta
     8761 cgactttatg ggatacagca aggaacacca gctgccgggg tagaaagcta tgatttcagt
     8821 ttttcaaaac tggaagcaga agtaagcggc tctagcgatt tgcttgcatc gatcggtgac
     8881 agtattgctg taccgatcaa tgtctcaggt atcaatcatc gaacaactcg taaaattgaa
     8941 ataccagtag aagaggggtt aagtatcaag cccgaatcag ttagtgtgga gattacaccc
     9001 gtgttgcaaa cgtcaagcag tagctctgct tcgacgagca gtcggacaca agaaaataca
     9061 agtacgagta gaagtatttc atctgctgat tcaggaacgg aaccatcgac aacaaccatt
     9121 tcttccagtt cggaatctgg gacaagcagc tcgaccacaa gtacagaaga aacatcagaa
     9181 acaagcgaat caacacaaat gagcagaaca aattcctcag aaaattaagt taaaggagta
     9241 attttacaac tatgggtaaa tattttggaa cagacggcgt acgcggtgaa gccaacaagg
     9301 aacttacgcc agaacttgca tttaagctgg gacggtgcgg aggttatgtc ctaagccagc
     9361 atgaagaaga aggaaaacgc ccacgcgtgt tagtagggcg ggatacaaga atctcaggtc
     9421 aaatgttaga agaagcgctg atttcaggac ttttatcggt gggtatcgaa gtgttccagc
     9481 taggtgtgat ctctacacca ggtgttgctt atctgacacg tttgcaaaaa gcaagcgcag
     9541 gggtcatgat ttctgcttct cataatccag cacaagataa tggaatcaaa tttttcggtg
     9601 cagatggttt taaactagta gatgaccaag agctagaaat tgaagcatta ttagatgctg
     9661 aagtggatga attgcctcgt ccatcagctg aaggattggg aactgtagaa gaatttccag
     9721 aaggcttatt gaaatattct caattccttg tacaaacgat caacggtgac ctttccggtt
     9781 tgaccatttg tgtcgatgca gcaaacggag caacttcaac aagtgttaat cgtttgtttg
     9841 ccgatcttga aacagatttt tatacaatgg gcacaaatcc agacgggtta aatatcaatg
     9901 acggcgtagg ctctacccat ccagaacgtt tagctgagat ggtcgttgaa aaaggagcag
     9961 atgctggact agctttcgat ggcgatggcg atcgtattat tgcggtggat gaacttggaa
    10021 acattgtcga cggtgataaa atcatgttta tctgtgctaa atacttggca gaaaaaaatc
    10081 gattgaaaaa agatacgatc gttacgactg tcatgagtaa cttaggattc cacaaagcgg
    10141 tggaaggaat cggtttgaaa gatgtcgtta cacaagttgg tgatcgttac gttgtagaag
    10201 aaatgcgcaa aaacgattat aactttggcg gcgagcagtc tgggcacatg attttcttag
    10261 attttaacac aactggagat gggatgcttt caggaattca gttactgaat atcatgaaac
    10321 agacaggtaa gaaattatca gagcttgcag cagaggtaac aatctatccg caaaaattag
    10381 tgaatatccg cgtaagtgat aaaaacggtg cgatggatgt tccggcaatc aaaaaagtga
    10441 ttgaagaggc agaagctgaa atgggatcag aaggaagaat cctagttcgt ccttccggta
    10501 cagaaccatt acttcgagtg atggcagaag cgcctactga tgagaaagtt aattactatg
    10561 tcgataaaat cgcggcagta gtcaaagaag aaatcggact ggattaagag atattttcat
    10621 aaaaaataaa agaacaagga ccagtgtcag atgtctgaca ctggtccttg ttcttttatt
    10681 attctactgt cactgatttc gctaaattac gtggtttgtc aacgtcgaat ccccgtaata
    10741 gcgttgcgta atatgcaagt aattgagtcg gtacgactgt ggttagagta gttagataag
    10801 ggtggacagt tggtagcacc agctggtcac tttccttttc taatccctct gagacaataa
    10861 ctagtgtttt agcgccacgg ctttcgactt ctttcaggtt tcctcgagta tgcgcaccag
    10921 tcacttcttc actgatgatt ccgatcactg gtgttccttc ttcgatcaaa gcaatcgttc
    10981 catgtttcaa ttcgccagcg gcaaaacctt ctgcctggat ataactgatt tctttcaatt
    11041 tcaaagcagc ttcaagagaa acgttgtagt cagcaccacg tccaatgtaa aaggcatttc
    11101 ttgtatcgct gagatatact tctgccaact cttctaaata atctttttgg gcgatgaccg
    11161 attccatcgc attagcaact aaactcaatt catgagtgat atcgaataca agagaagttt
    11221 caaattcttt tttatcaccg atagctttgg ctaataaagt tagtacagct atttgtgcag
    11281 tataagcttt cgttgatgct actgcgattt cgggtccagc atgcaatagc agtgtaaagg
    11341 atgcttcccg cgaaagagta gaaccagcaa catttgtaat cgtaagtgaa gggtagccga
    11401 gacggttgat ttttaccagc acttggcggc tgtctgctgt ttcgccactt tgactcaaga
    11461 aaataaagaa tggttttttt gaaagaagag gtgtgttata gccaaattca ctggctaaat
    11521 gaacctcaac aggaatctgt gtcaattttt ccaaagtctg tttacctgct aaccccgcat
    11581 ggtaactagt tccgcaagca acgatataga ttcgatcgct atcagacaat gtatcgacca
    11641 gcttttgatc aagagagaca cgattgtctt ctgttacgta tttttgagcg atacgacgca
    11701 tgataatagg ctgttcgtcg atttctttca gcatataaaa aggataggta cctttctcga
    11761 tatctgaagc atctacttgt gcttcgaatg aagaacgttc tacgatgtca cctgcaaaat
    11821 tttgaataat aactttttct gatgtgacgg taaccatctc ttcatcttca atctcaacga
    11881 attccttcgt atgagcaagc atagccattg catcgctggc aatcacattg aatccatcgc
    11941 cgagtccgat taatagtgga cttttatttt tagcgacgta aataacatca ggtgctgtcc
    12001 gatcaatcaa agcgaaagcg taagaacctt tgatgacccg caaagctttt aaaaacgcct
    12061 cttgcgttgt agtatccttc gcgaaagttt caattagatg agcgataatc tcagtatcgg
    12121 tctcaccaat aaagtggtca tttgctaaat aagcaatttt aagttcttca aagttttcga
    12181 ttacgccgtt gtgaacgagc acgaagcgtc cactttggga agtatgtgga tgggcgtttt
    12241 cttcagaagg ttgcccgtga gttgcccatc gtgtatgacc aatacctata tttcctgtag
    12301 tatcaatatt tattttgttt ttcaaatttt gaattctgcc ttgtgctttg accagataat
    12361 cagttgtgcc atctgagaca aagataccag ctgaatcata accacggtat tctaattttt
    12421 ctagaccgtc aatcaatcct ggtgttacat tttttaatcc aatcattcca acaattccgc
    12481 acataaaaaa catcctactt tctataatga attcgtatga atgcttccag ctgcaggaaa
    12541 atccttttgt tttatctttg cagacatttt ttgtggattt tctgtagagt gcagtcccta
    12601 gggccatccg ccgaatcttt cgataaaccc tctcctcgtc atctgtttac acagacctgg
    12661 cg
//
LOCUS       DAYYHX010000071        12642 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000071 DAYYHX010000000
VERSION     DAYYHX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.071"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1089)
                     /locus_tag="ACHA6Y_001482"
     CDS             complement(<1..1089)
                     /locus_tag="ACHA6Y_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8987580.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIE"
     gene            complement(1211..1807)
                     /locus_tag="ACHA6Y_001483"
     CDS             complement(1211..1807)
                     /locus_tag="ACHA6Y_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8987581.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(2007..4649)
                     /gene="alaS"
                     /locus_tag="ACHA6Y_001484"
     CDS             complement(2007..4649)
                     /gene="alaS"
                     /locus_tag="ACHA6Y_001484"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM8987582.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            5037..5468
                     /locus_tag="ACHA6Y_001485"
     CDS             5037..5468
                     /locus_tag="ACHA6Y_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8987583.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(5513..6862)
                     /locus_tag="ACHA6Y_001486"
     CDS             complement(5513..6862)
                     /locus_tag="ACHA6Y_001486"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8987584.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(6932..8095)
                     /locus_tag="ACHA6Y_001487"
     CDS             complement(6932..8095)
                     /locus_tag="ACHA6Y_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8987585.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            8224..8547
                     /locus_tag="ACHA6Y_001488"
     CDS             8224..8547
                     /locus_tag="ACHA6Y_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987586.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(8730..10079)
                     /locus_tag="ACHA6Y_001489"
     CDS             complement(8730..10079)
                     /locus_tag="ACHA6Y_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8987587.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(10148..11101)
                     /locus_tag="ACHA6Y_001490"
     CDS             complement(10148..11101)
                     /locus_tag="ACHA6Y_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM8987588.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(11203..12393)
                     /locus_tag="ACHA6Y_001491"
     CDS             complement(11203..12393)
                     /locus_tag="ACHA6Y_001491"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM8987589.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         3623 a         2674 c         2200 g         4145 t
ORIGIN      
        1 ttctatttga ctacgtaaga ttctttctag ttctgtatca acaaactgga atttctcagg
       61 gaccactaca tcaaattgca gcgtttttcc ttgatgaatt actctaaaat catgaatatt
      121 aagccctgta cctgtttcaa gaagaatttg ctttaactgc cgcaagatat tatagtattt
      181 ttcgttatca acaggcattg gatccaagtg acagacaagt tccacatcca aagacttttt
      241 aaagtctttt tctatcagat caataatctt atgagccaca ttcaaattca ttgattcatc
      301 tacttcgatg tggactgatg caaagcgttt attgggtcca tagttatgca ccaataaatc
      361 atgatagcca agtatatcat cgcaagcatt taaatgctct tccatttctt caatctctgc
      421 tggcgttggg cgacttccta atagttcgta tacaaaattt cgaagcatcg tcaatccact
      481 ataaaggata tataatgcta aagcaaaacc tatatatcca tcaatacgcc atccagttac
      541 ccattcgatt gctgctgaaa gcaatacagc cagtgtagta aagacatcat tataactatc
      601 tgttgctgta gctctcaatg tttcagaatc aatggattgc gcaataacct gatacatttt
      661 gccttgtgca aatttcaaaa agatggaaat gattaggaca aaaaagacga tgggtgttaa
      721 agagacattt tctggatgga tgattttttc agcagaagac tctaaaaatt gaaatcctac
      781 ataagtaatg atgatcgaaa caaataaccc actgatatat tcgaagcgtt cgtgtccata
      841 aggatgttcc tgatctgctg gctttgcagc aattttaaat ccgatcaaag tcaatatgga
      901 tgaagcagta tctgatagac tgttcactgc atcagccata atggagacac ttcctgaaaa
      961 aagcccgatc attagcttag agacaaacaa gatcagattc gtaaccaatc ctaagatacc
     1021 cgctagtact cctttttttg tccgctgttg cggtatttgt ttcttttttt cttcttgttt
     1081 cttagccaag ttttttaccc cttctagttt gcttgttgct caggtaaaaa gatggagagg
     1141 acgattgttt tcctctcctc tcttcctttt tctatctatc gtgcgtccta ttactattaa
     1201 aagatggtca ttattctggt ttcaacggtg ttgctgcaaa taacgctttt gctagtaatg
     1261 gtgttatgat tcccgcacct attgcttctg gtatcccatt gattgctgca acccctgcta
     1321 aggttttaaa taaaagagca gagtctacac cataggcatc tgccgttgca cctgtataaa
     1381 ataatcccat gaatcctaac actaagactg tattgaccaa tgaacccagt acccctgcaa
     1441 aggctgagct gatcgttatt gatttatttt tttgataaat ccagtggaaa accaagcctg
     1501 ctactaagcc aacaagtact ctgggaacta ccgagataat cggattcgtg aacactaatg
     1561 tatcaaatgg cgttgttggc atggcatagg cacgaacaat tgtagcgatt ccccaaaaca
     1621 agccaatcac catgccatct tttgggccta atactactgc agcaataatt actgtgatat
     1681 ggataattgt taaactaatg aatcctaatg gaataaaccc cagccaagga accatcgctt
     1741 gtacgatgat gattgctaac aagatcgcac gtaagactag tcgaaaggtc ttatttctac
     1801 tattcatttt ttcacccctt tttatttata ctaatcgtaa aaatttctgt cactttcgtc
     1861 aactaaagac catccgaatc cgaccatttg attagatgcg ccatccaatt gaaaaaagta
     1921 tgagggcaag acttgtttgt cttcgccccc ataatcatgt ttcgttcaat gtttgaactt
     1981 ttgtttttct gtcaaacgta aacgccctat tgttgcgcaa cccattcttt cgcagcttta
     2041 agtgcatctg gaataccagc aggatttttc ccgccagctt gtgccatgtc tggacgtcca
     2101 ccaccgccac cgcctacttt cggtgcgatt gctttaatca agtcgccagc ttttagtcca
     2161 gcttcatttg attctttcgt cattgccgct agcagactga cttttccttc ttgttcgttt
     2221 gctaaaacaa gaatatctga aatatttttt tgtttccatt ggtcagctaa ttgacgcagc
     2281 tgattcatat ctttcacttt tacttgtgct gcaatgattg tttttccatt gatcgtttct
     2341 ggatctttga atacatcacc agcttgttga ttcgctaact tgctagctaa ttgttcattt
     2401 tccttttgta gctctcttac ttgctgttgc aattgttccg cttttgaaac aacttctttc
     2461 aattgaggtg atttaacaat tgttgctact tgttttagtt gtttttcttc ttcttcaagt
     2521 agttcgtacg cttctttact tgttacagcc tcgattcgtc gcacgcctgc accaattcct
     2581 gattcagaaa cgattttgaa gattccgatg ttttctgtat ttgctacatg tgtgcctccg
     2641 cacagttcga ttgaccagcc gccaatattt actactcgaa cgtcatgtcc gtatttttca
     2701 ccaaacaaag ccattgcacc catgtttttg gctgtatcga tatctgtttc aattgtttca
     2761 actgggatag cttcccagat tttttcgttg acgattcttt ccatttcagc taattcatct
     2821 gaagtgattt gaccaaagtg ggtaaagtcg aatcgtaaat gtccaggagc gactaaagaa
     2881 ccagcttgat ttgcatgctc gccaagtact tctttcaatg ccttgtgcag tagatgggtt
     2941 gctgtatggt ttttgatgat ccgattgcgc atcgattgat cgatcaatag atgataggtt
     3001 tgtccttcac tcattggtgc ttttacttct acggtgtgca taaattgacc attaggcgct
     3061 ttttgaacat ctgtaacaac tgctacgatt tcgcctactt cattcatgat ccatccttga
     3121 tccgctactt gcccacccat ttctgcatag aaaggtgttt gatcaaagat catttgagct
     3181 tcaccttctg cgacttcatt caaaagagac tcacctttaa cgatgaccaa taattgacta
     3241 tctgattctt gttctgtgta tccaatgaat ttactttcta ctttgatatc tgtcaataaa
     3301 gcagattgta cccccattga tttttcagta ctgcgggcag agcgagcacg ttcgcgttgt
     3361 gcatccattt ctttttcaaa accagcatga tcaactttca gtccttcatc ttctgccacc
     3421 tcttccgtta attctaccgg gaagccatat gtgtcgtata gtttgaagat gtcttttcct
     3481 gctagagtcg tcccgttttc gttttttact tcttggatca cttgatttaa gatttccaaa
     3541 ccttcattga ttgtttcatt gaaacgttct tcttctgtac ggataacttt ttcaataaat
     3601 tctttttgtt gaagtacttc aggataataa cttaccatga tttccccaac aacagggact
     3661 aaacgatata agaatgcttg ttcaatacct aattttttcc catgcatgac agcacgacga
     3721 agcaaacggc gtaagacata ccctctccct tcattcgatg gcaatgcacc gtcgccaata
     3781 gcaaatgaaa gggcgcgaat atgatctgcg attactttgt aagaaatatc tgttactggg
     3841 ttttcaccat attttacttg tttgcttagc ttttctacgg cgtggataat aggcataaac
     3901 aagtctgtct caaagttcgt tgacgcatct tgaacgattg agaccatgcg ttccaatccc
     3961 atccccgtat caatgttttt atgaggaagt ggttcaaatg tgttatcagg cttatggttg
     4021 aattctgaaa atactaagtt ccagatttct aaataacgtt cattttctcc tcctggatag
     4081 ttttcaggat cgtcttctgc taaatcgtta taagcttctc cacggtcata gaaaatttcc
     4141 gtatccggac cacatgggcc tgcaccgata tcccagaagt tgtcttctac atcaataatg
     4201 tgatctagtg gcaggccaac ttcttcatgc cagatgcgtt ttgcttctgt atcttttgga
     4261 taaactgtta cataaagttt ttctggatcg aatgccatcc attttggatc agtcaaaaat
     4321 tcccatgccc agtggatggc ttcatttttg aaatagtcac caatcgaaaa attacccagc
     4381 atctcaaaca tcgtatggtg ccgtgctgtt ttaccgacat tttcgatatc atttgttcga
     4441 atagattttt gggcatttgt gattcttgga ttttctggca caactgaacc atcaaaatat
     4501 tttttcaatg ttgcaacacc agagttgatc cacaataagg ttggatcgtt cacggggatc
     4561 aaagacgcac taggttcaat cgtatgtcct ttagattgga agaaatcaag aaacatttgt
     4621 cgaacttgtg cactacttaa ttgtttcatg tcattacctg ctttctataa attaaataca
     4681 aaaaaacacc atcacagcca aggacgaaaa ctcgcggtac caccttgatt gcaatgatgt
     4741 gtaatcatta cctcttaaac tcgataacgc tgagctgcgt cggtatttct accaaaatag
     4801 aaaaagggag caatttgatt agtgactatt cttttttcag ccaagaaaga attttctgtg
     4861 agtcttaaaa aacaaatcat atccttttcg tacttaatta gtatacgcat tctattgatc
     4921 actgtcaata tctcaatttt tattttcatt tccttttggt catactccac gatttttggt
     4981 acgataagaa taaaggaatc aatacatctc tgctattcat gacaaggagg aatatgatga
     5041 aaaaaatagt tggagcggat gaatggatac gcgcagctag ctgctttttg agatatcaag
     5101 tttttgtatt agaacaaggg attcctgcag aactagaatt cgacggaaaa gatatcccgc
     5161 aacaaaccta ttatctattt cttgatcaaa aaagaccagt agctactttg cgttatgaaa
     5221 agaaaacacc tacttgcttc aagccggaac gattttgcgt agcaaaagaa taccgaggaa
     5281 aagggttagg cggtcagttg cttgaagctg ctgaaaaacg ggcggtttca gaaggttgtc
     5341 agactagtta tgtagtagct gaaatgacgg ctgtagattt ttatgaaaaa caagggtatc
     5401 atacttgcac agaacctttt attgaagatg aagtactttg tatcggaatg aaaaaaagtc
     5461 tagcttaaaa acaagagatt gtgacacgcc ttttgtcaca acctcttgtt ttttatttat
     5521 gtgaattttt cttattttta cgagcagtac ggctttgctg tctgcgttcg attttgcgtt
     5581 cttttttatt tttttctgca attgcccaat cgattttttt cttataacca ggcttgatct
     5641 ttttcttttt cttcttcaca agaccaatca gcgtgggatc taatacatct tttgattttt
     5701 ctcgtttggc acgtctatta cgatcgtatg ttttgacgat ttccccgttt ttgatttctt
     5761 ttggttcaaa tgagacgccc aatttttcta tcgcatcaat ggcttgctca tcatttggcg
     5821 aatataacgt aatggccgtt ccttttaagc cattacgtcc agtacgtccg acacgatgga
     5881 taaagaagtc tagatcttct ggaatttctg cattaataac atgagagaca ccttcgatat
     5941 cgatccctct agcagctaaa tcagtcgcta caacatattg atactccaga ttctgtactt
     6001 gtcgcatcac tcgttttctc tctcttgggg tgatgtctcc atgaatcttt gctactttca
     6061 agccttggtt tttcaagtaa tcggcaattt catccacatg ctgttttgta ttggcaaata
     6121 cgattgctaa ataaggatga ccaattgtca gcagttgata aatcatttga ttctcatctt
     6181 taccttttgt tgaaatcagc caattttcaa tgtcttcaga aataactgct tttggtttga
     6241 tttcttcaat gattggattt tccatgtatt ttcgtaagaa tggacgaagt ttttctggaa
     6301 tagtggcaga aaaaactaaa aactgcagct tttctggtaa tcgacttgca atttggtcta
     6361 cttcatttaa aaaacccata tccaatgtca tatccgcttc atcgacaaca aatgcaaatg
     6421 cggtgtgaat cttcagcgct tgttcattca tcatgtctaa tattctacca ggcgtgccta
     6481 ctacaacctg tggctgttgg tgatgcagac gatttaattg acgctgtttg tctgtaccgc
     6541 cgacaaaatt cgagacacgg atctccggtg tagaaaattt agcgatttgt gtagctgctt
     6601 catagatctg tgcagctagt tccctacttg gagctgtaat aacaacttgt acttcctctt
     6661 tttctgcatt gattttttca aacaaaggca acaaaaacgt atgtgttttt cctgtaccag
     6721 tttgagattg cccaatgata ttttttcctt ttgcaattaa aggaatcaaa cgttcctgga
     6781 cctctgtggg ctgtttgaag ccctttgcgg ttaacgcttc attgataaac ggtttgaagc
     6841 ggaatatatc aaaattattc attcgtgcac ctcttctctt taaattatca cgactttggc
     6901 attataacac agacaaagcg tttttgctca gctaatttcg agaatcatca tcctttgttt
     6961 ggccaatcgt gctaacagta tatgtgtgac tagctttttc ataaaacaga atccgttttt
     7021 ttctcaaaaa tgatagcaag atatttaaaa taaaaatacc ttgtagtaac aagacaccga
     7081 gagaaatgaa tactgcactc atctgcaaca ccgtattcgt tgtcgaattc atgatttgct
     7141 gaagcaactg ccccaaccag cttaaaccat aaaaaagaaa gtacagatac ccgtagcgga
     7201 tcatcaatgc ttttaaagaa attcgttcgc ggtcttcttc caccaaacgg atacgcacaa
     7261 tttttttgcc aagtgtccat ccattggtta taaacggtac aatcataaag tataccccta
     7321 cttctaataa aagccaccag atatgagaag taactgcccc tgaatcgaac gcaattttaa
     7381 agaaaatcgc cgctaccaaa ttaataagag ataaaaatgt ccagtcgatc aaataagcga
     7441 ccaagcgtcg agtcaatgag acagtctttc ctttttcata agaaagttca tccattcgtc
     7501 ttcttgtagg aagcataaat gtaaataacg gcgtaacgac aaaaccaatg attccaccca
     7561 atgtatttgt cactaaatca ttcacatcaa ataagcggta tggtcttggg tagataaaat
     7621 aaagtccact tagctgcgtc aattcaaaaa atagagaaag gcagaaacta gcaatcatcg
     7681 ttttcataaa agaaagccgg aaataatatc ttaaataaat gccaaacggt atgagtaata
     7741 aaacattaaa agctggttcc aaaaatacac tctgcttcat tgccggcagg taggtactag
     7801 gttcagacaa gaccaatacc gtgttgtctc tgaaatgttg ccatgaagca aataaatgca
     7861 gctccatctt tggccctgtc atttgggcaa ccgtctcctt tgccggaagt ggtaagatca
     7921 ccaaaaaata cgcacacaat aaataaaaaa cgaaagaata caaaataact gcccttgtta
     7981 ctagaaatgt tccatacttt cgatattcat aaataaaaaa aacagatgag atcgctaagg
     8041 ctaaaaatgg aaaaacaatc agcgcaaaac gaatcggttc gatataactt gccatatgac
     8101 tcctccttga ttctttgtat ctctcttatc ttagcagtcc tttgctgttt caagcaagta
     8161 acgaggctat tatttctcat ttctttcaaa gtaaagtaag ataaacacaa agggggtgtc
     8221 gagatgctga atatgtactt tgtttttggc gtacctattt ttttactgtt cctttatgct
     8281 acgatcgctt atgtaagaaa aagaactaca attcattatt taggatttat tctcctgatt
     8341 atttcgggtt tcatgctagt cttcaatctt cagacttggc agcaagcact tcttgaaatg
     8401 gataaaatga ctccacatgc tttgtctaaa gtgcttggct accctgtcta tctgatttgg
     8461 cttcccattt tcatttctgg ttgcttggtt ttattaaata tttatcgcgg cgtacgccga
     8521 atagttcagt tacgcaaatc aaagtaaaag acagaaatcg gttgagcccg atttctgtct
     8581 tttactttgc tatttcatcg tagacaaaac gacatttacg tgatcaaaat taacgagtac
     8641 ttttgacatt tcttccacgt actcctcatt taaataaaat attttgtttg gcagagaagt
     8701 gcagttctaa taaaattcga atgttttagc tatctacgtt ttaaatagag ataaatcggg
     8761 ataccagcaa gtgtcaatac tatccccatc gaagctaaaa aggtctgagt gataagcgtg
     8821 cttgatacga taaaagtccc tccaatgatt gctactagtg gaataaacgg atacattgga
     8881 actttgtatg gtcgaaacaa atcaggttct tttttacgaa gaagaatgac accaacaaag
     8941 accatcgtat aaaaaatcca aatgacaaag atcagcatat ccgttagtgt atcaaaacca
     9001 ccgatcatca tcatgccaat agcgatgact agctctaaga tacctgcggc tacaggaact
     9061 ttagttttat catgaagttt agcaaagagc ttgctaaaag gcaggttgtt ttcttttgcc
     9121 ataacataag ggagacgcat tccagtaagg gtatagccat tgatggttcc ataaacagaa
     9181 atcaagatac caactgtgac cagtcttcca ccgaagccgc caaaaatcat ttttgcaacg
     9241 tcacttgccg cattgctgtt tcctgcgact ccgtcgatag atgcggttct taagaacacc
     9301 gcatttacta ataaataaac gatcatgatt ccgatgattc ctaaagaaat tgcttttggc
     9361 aaatctttcg ccggcttttt caattctccc gaaatatttc ctacgtgtat ccaaccgtcg
     9421 tatgcgaaca tcgttgctag caagcccgct cccaaggctg agaaaaatga aagattttct
     9481 cctgcttgga tcggaaacag ttggaagtct actccttctt gtcggaacaa accgaaaatg
     9541 acgatcacaa acaaaggaat aagcttgcag acaagcgtaa tcgactgaaa agcccctcca
     9601 gctttagaac ctaaaaaatt gatcaacagg atcgatacag cagctgtaac agctacggga
     9661 acaatcattg actgggaaag accaaataga ttgacgaact gtgtaccgaa aatgatcgac
     9721 aaggctgcta catttgctgg aaaataaatc acgacttgcg cccagcccaa taaaaaagct
     9781 gctgtatttc cgtaaatccg ttcgatatat ttaatcatcc ctcctgtttc tggaatagca
     9841 gctgccagtt cagctcctgt tagtcctgca cagacagaaa tcataccacc gagaaaccaa
     9901 gaaaacatat gcaaactggc ggatcctgta acttctgcta cgcttgcggc tttaaaaaat
     9961 acccctgcac cgatcactgt gcccataaca gtcgataaag cagtaaaaaa tcccatcgta
    10021 cgttttaatt cattttctat ttttacttct tttactggcg tggcttgata ttcttccatc
    10081 ccttgtcccc tttcatacca tagccaatcc caaaagtata aaaaactttt tggatgtggt
    10141 tcttgtttta taccgtctct accaattttt ctttcagttt ttccaacata tcctttgtca
    10201 tcacatctaa gtcgtatttc ggcgaaaagc cccattcctc ttgcgcacaa gaaacatcta
    10261 gtgaatttgg ccaagattct gctattgact gacgtacagg atccacatca taatccattt
    10321 caaaatctgg tatgattttt tgaatagatg ctttgatctc ttccgggtca aaagacatcg
    10381 ccgatatatt gaaggcattt cgatgaacca atcgatcagg atctgcattc atgagtttta
    10441 taatggcatc gatcgcatct ggcatataca tcatatccat tgatgtacct tgatcaatga
    10501 aactagtgta ctttccttct ttgattgctg aataataaat atcgactgcg tagtctgtcg
    10561 taccgcctcc tggcaaggtt gcataagaaa tcaatccagg gaaacggaca ccacgagtat
    10621 ctacgccata tttcacataa taataatcac atagtagttc tccagctacc ttcgttaccc
    10681 catacatggt tgtaggacgc tgaagagtat cttgtggggt atgatctttt ggagtatttg
    10741 gaccaaacgc gccgattgaa cttggcgtaa aaaatttcag ctggaattct ctagcgactt
    10801 ccaatgcatt gaccagccca gtcacgttca aattccaggc taattgtggt tttgcttctg
    10861 caacagctga caaaagagca gccaaatgaa tcaatgtgtc cacttgatat tgttctgcta
    10921 tgctgcgcat tttttcgtaa tccattacat ctaggatctc aaagatatcg tttttctcaa
    10981 tgacttcagg catgcgtata tctgtcgcaa tgacattttc actgcctagt tcttgacgta
    11041 gccgtgtgat caactctgaa ccaatttgtc ctaaacatcc tgtcaccaat acttttttca
    11101 tgatgatcct ccctgatctt ttttactact atcttcagat gattaacaat atatgcgtga
    11161 acatgttctc taattttaaa tagacgaaaa aactaaattt agttaaataa tttccatttc
    11221 ttttcctatt ttttgataaa ctgctactgc ttcatcaagc atttccctcg tatgttccgc
    11281 agtcggcata tttctgatcc tgcctgtccc aagaggcaca gttggataaa cgattggttt
    11341 agcataaatc ccatgttcga tcagcttctt agagaaagtc tgagtcactt tttcgtctcc
    11401 caaaatgact ggagtaatag gtgtttcaga cataccgatg ttatagccta cttttttcaa
    11461 ttcttcttta aaataattag cattttccca aactttttcg acaagttccg gatgttcttg
    11521 catcaaagta atcgaagcca atgcagctgc agctgcacct ggagttagcg aagtagaaaa
    11581 taggaatggc cgacttctag ctttcaacca gtcgattagt gttttcgatc cagctacata
    11641 acccccaaca acacctatag ctttcgataa cgtgcccatt tgcatatcga ttttgtcgct
    11701 taatccaaaa tgcttgacgg ttcctgcacc ttttccagtt acgcctgagc cgtgcgcatc
    11761 atcgacgtag gtgatcagtc catatttttc aacaatcgga ataatttccg gcaatcgtgc
    11821 aatgtctcca tccatggaga aaacaccatc tgtgatatac atgatttttt tatatttctt
    11881 actttctatc gcttcctttg cttttttctc aaggtctttc atatcttgat gtttaattcg
    11941 gatgattttt gctccggata agcggcaacc gtcaataata gatgcatggt ttaattcatc
    12001 cgataagata gcatcttctt ttgtcattac tgcagagata gcgcccatat tgcagttgaa
    12061 tccagattga aaggcgattg ctgcttctgt ccctttgaat tctgcgattt tttcttctaa
    12121 ttgctgatgg attttcagcg aaccattgat cgttcggact gctcctgctc ctacaccata
    12181 agtttcggtt gcttcaatac atgctttttt caattcttcg cgattggcaa atccaagata
    12241 attgttggat gctagattga tcatcttttt tccgtctacg atgatacaag caccattttc
    12301 actttctagt atgtcgatcg tattgtataa ccccttttct ttcagttgat ccaattcttt
    12361 ctctaaatac attgttagtt ctttggaaga cattgtctcc acctccatat tcatctgtct
    12421 tgcagaacat accaaacata tatcgtgtca ttcatctaca aagcaagtat gctatatttt
    12481 tgataacgct tacactgatt atgagaatta tttaaccaca ttttttattt acagtatctt
    12541 tttactttca aatgacttat atagtatgaa caattacctt ttagataatg atctatttta
    12601 ctctacctgg gcagataaat acaacacaac cattgataga ag
//
LOCUS       DAYYHX010000072        12493 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000072 DAYYHX010000000
VERSION     DAYYHX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.073"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..459)
                     /locus_tag="ACHA6Y_001492"
     CDS             complement(97..459)
                     /locus_tag="ACHA6Y_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987590.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(533..913)
                     /locus_tag="ACHA6Y_001493"
     CDS             complement(533..913)
                     /locus_tag="ACHA6Y_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM8987591.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            995..2170
                     /locus_tag="ACHA6Y_001494"
     CDS             995..2170
                     /locus_tag="ACHA6Y_001494"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM8987592.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2247..2831)
                     /locus_tag="ACHA6Y_001495"
     CDS             complement(2247..2831)
                     /locus_tag="ACHA6Y_001495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8987593.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3032..4030
                     /locus_tag="ACHA6Y_001496"
     CDS             3032..4030
                     /locus_tag="ACHA6Y_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8987594.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4142..4651
                     /locus_tag="ACHA6Y_001497"
     CDS             4142..4651
                     /locus_tag="ACHA6Y_001497"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM8987595.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4681..5340)
                     /locus_tag="ACHA6Y_001498"
     CDS             complement(4681..5340)
                     /locus_tag="ACHA6Y_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM8987596.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5342..6106)
                     /locus_tag="ACHA6Y_001499"
     CDS             complement(5342..6106)
                     /locus_tag="ACHA6Y_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM8987597.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6114..6791)
                     /locus_tag="ACHA6Y_001500"
     CDS             complement(6114..6791)
                     /locus_tag="ACHA6Y_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM8987598.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6828..8213)
                     /locus_tag="ACHA6Y_001501"
     CDS             complement(6828..8213)
                     /locus_tag="ACHA6Y_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM8987599.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8423..8507)
                     /gene="ffs"
                     /locus_tag="ACHA6Y_001502"
     ncRNA           complement(8423..8507)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHA6Y_001502"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8530..9045)
                     /gene="tadA"
                     /locus_tag="ACHA6Y_001503"
     CDS             complement(8530..9045)
                     /gene="tadA"
                     /locus_tag="ACHA6Y_001503"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM8987600.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9152..10009
                     /locus_tag="ACHA6Y_001504"
     CDS             9152..10009
                     /locus_tag="ACHA6Y_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8987601.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10090..10425)
                     /locus_tag="ACHA6Y_001505"
     CDS             complement(10090..10425)
                     /locus_tag="ACHA6Y_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987602.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10492..11862)
                     /locus_tag="ACHA6Y_001506"
     CDS             complement(10492..11862)
                     /locus_tag="ACHA6Y_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM8987603.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11940..12476)
                     /locus_tag="ACHA6Y_001507"
     CDS             complement(11940..12476)
                     /locus_tag="ACHA6Y_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8987604.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3701 a         2546 c         2100 g         4146 t
ORIGIN      
        1 atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa gaagtttgaa
       61 aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca ctccctctac
      121 ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat atccttcgcc
      181 taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa cattttcctc
      241 tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga tatgaactat
      301 ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa gttccgctaa
      361 cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac catcctccaa
      421 atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct cgatcaactt
      481 gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact ttttatttct
      541 tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt aactgctccg
      601 caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt ctgcgatatg
      661 gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt ttggaatatt
      721 catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat gaatggatat
      781 ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc tcgttatcaa
      841 gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt atttgtccaa
      901 ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa atagctcaaa
      961 aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata aacagatttc
     1021 aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc aaatggatga
     1081 cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg tagctaaagc
     1141 gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg ataaactata
     1201 tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga aagaaaatat
     1261 cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt ttacaaaacc
     1321 aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta ttatagaaac
     1381 aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt tcatcatgga
     1441 cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga ttttatgcaa
     1501 tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt taagcgaatt
     1561 gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt taactttatt
     1621 cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc tactagttgc
     1681 ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa tggtatttat
     1741 caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc aacagcacga
     1801 aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg gagaatggtt
     1861 aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt tttttgaaca
     1921 gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt ggttagattt
     1981 taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga aaggaaaagt
     2041 cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc gcttgaatat
     2101 tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg ctttagagac
     2161 aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt tcgtcctttt
     2221 tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc aatcactaca
     2281 tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc tactgtgtcc
     2341 atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg agttccgcca
     2401 cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg tccaagcata
     2461 ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt gtttggacct
     2521 gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa ttcatcttca
     2581 aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc gccgccttgg
     2641 atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc ttttttggaa
     2701 agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg aacagtaatg
     2761 tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa atctagttgt
     2821 gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa taacataata
     2881 cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac tagatacatt
     2941 ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc tcaattaaat
     3001 tctgatatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta caatcatcgg
     3061 tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag ctaaaacaaa
     3121 aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc ccgaaaaata
     3181 tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg atcaattaga
     3241 aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc tctcgcttac
     3301 tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt ccaaagcagt
     3361 cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag ataatgctga
     3421 atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt atgctggaaa
     3481 aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga tgttagagcc
     3541 tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc acgaacatag
     3601 tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca tcgatggatt
     3661 atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa gtgacgagac
     3721 gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta atttagaaca
     3781 tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat cagatatgtc
     3841 tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag gaaaagtgaa
     3901 actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt tacactatat
     3961 taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata aaaatcttcg
     4021 tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc agtttcttgc
     4081 atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg ggagcaataa
     4141 tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga aggaacgtgg
     4201 cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact atatttctga
     4261 tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc gtgaagtaca
     4321 taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa aattgatgag
     4381 tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg agatcctagc
     4441 cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt atatcgacaa
     4501 ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg tccatacctt
     4561 cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag cacacaaaga
     4621 accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga aaacgtctat
     4681 ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga aaagaaaaac
     4741 agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga aaaattcttc
     4801 tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat cgttgttgaa
     4861 aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga aaaaaccgaa
     4921 aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa gccgatcatt
     4981 cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa acacagcata
     5041 atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag cagaaactgg
     5101 gaaatctggc aagctgagga tggttttgaa gggattattg agataattca ttaggtgatc
     5161 aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt gcaaatgtcc
     5221 gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca gactgatcag
     5281 tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg aatttttcat
     5341 attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata ggcaacccag
     5401 gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata ttattgcgta
     5461 tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct tctttttgca
     5521 gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg atgtagatcg
     5581 gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca ggaagaaggc
     5641 cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca cctttttgaa
     5701 tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag cctaccacta
     5761 catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag tcaatcaatc
     5821 ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata aaatcgattg
     5881 ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac tcatctgcta
     5941 gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac aagaaaggta
     6001 attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt tgtgtcccac
     6061 gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat ccactactct
     6121 tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg ttgttcttgt
     6181 ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat aaccggtttt
     6241 ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt atggtttttt
     6301 ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc gatgacaaaa
     6361 tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc gattcgctga
     6421 tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag ttgttcataa
     6481 ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg ttctccaagc
     6541 ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct ttcacctatc
     6601 aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc tgctttaggc
     6661 tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt ttcctgttca
     6721 gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg ttcattttgt
     6781 tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta tttgattggg
     6841 tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa caaaaattca
     6901 ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg atctacggta
     6961 gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac ttgatggtta
     7021 atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc gcgaaatccc
     7081 attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc taaaactaag
     7141 cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg cggcaacgca
     7201 gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa cagaccactg
     7261 tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc tacttggata
     7321 aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc tacttctttt
     7381 tcttttaata attcatgccc acgttctaga taactattta tttcttgttc atcttctgta
     7441 aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat ttttttctct
     7501 tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc ggctgctatt
     7561 tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga tcctaaaata
     7621 acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc gagatgactc
     7681 atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg caaaatatca
     7741 taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg agcagtaaat
     7801 gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg tttcgtccat
     7861 gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc aaactcagca
     7921 tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt gtttcctatc
     7981 gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc attcgttgct
     8041 tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat cgttgtcgtt
     8101 ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat ccttggccaa
     8161 ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc catgtaccct
     8221 ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg aaacaagctc
     8281 tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc gataaatcag
     8341 tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa aaaaacagac
     8401 ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc ttagtgctgc
     8461 ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt cggcaaagaa
     8521 tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt ttcgctggcg
     8581 gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa tgccaccttc
     8641 tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg taccggctgt
     8701 tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca aaatcatcgc
     8761 gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt ccaagcgcca
     8821 gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg tagtagcatc
     8881 ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca atacaaccac
     8941 tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca atgcaaggcg
     9001 catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc cttccctgcc
     9061 tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa cttttttttc
     9121 tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac ttgctcttta
     9181 cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt cttttgcaat
     9241 tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa ccagcctttt
     9301 gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc actatctttt
     9361 taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc acgtcgaatc
     9421 aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat ttcgctattc
     9481 tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa taaaccgtga
     9541 ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg attttgatac
     9601 gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg tccctttgtt
     9661 tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc acagccgtac
     9721 tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa aagatagtaa
     9781 gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga tcattcaaga
     9841 attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc ttcatcgtaa
     9901 tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc taaaaaaagc
     9961 caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa gatagattaa
    10021 aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt gttcaaaagc
    10081 tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta catcttcggt
    10141 tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt tgatttttgc
    10201 taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga tttctagtaa
    10261 tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc gagtagtcat
    10321 atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta cactggcagc
    10381 ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta tcagctcctt
    10441 tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca ctcagtgaaa
    10501 tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc caaatccgac
    10561 aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt gaaatgtttc
    10621 tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta tattctctat
    10681 cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt tgaaatctcc
    10741 taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta actcaggaac
    10801 ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat gaaaatcatt
    10861 tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac ttttttttcg
    10921 tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg caacaaaatg
    10981 tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac cagctacgct
    11041 tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct caatcgttac
    11101 tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg caaacgtaca
    11161 agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag acaaaatctc
    11221 ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta ttgcatttgg
    11281 atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc gatcaaaaaa
    11341 gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg cgttcgaaac
    11401 tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga tctcattcgt
    11461 cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac caccgggagc
    11521 cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc caacgatcat
    11581 tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact ttccattgac
    11641 agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc tatggtaagt
    11701 ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt ttttcacgct
    11761 tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa gacgatattt
    11821 tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt ttcgttagac
    11881 attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca tgttgctgcc
    11941 taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc gtttttatcc
    12001 caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg caaacttccc
    12061 aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt gtgacctttg
    12121 acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga ttttgcaatg
    12181 atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac aatgcttaaa
    12241 acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat ttcttctaat
    12301 atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta tgtagaacaa
    12361 ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc ttccacgtat
    12421 accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc catcattctt
    12481 acctccgtct ctt
//
LOCUS       DAYYHX010000073        12354 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000073 DAYYHX010000000
VERSION     DAYYHX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.074"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..1705)
                     /locus_tag="ACHA6Y_001508"
     CDS             complement(92..1705)
                     /locus_tag="ACHA6Y_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8987605.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(1846..2754)
                     /locus_tag="ACHA6Y_001509"
     CDS             complement(1846..2754)
                     /locus_tag="ACHA6Y_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8987606.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            2937..4061
                     /locus_tag="ACHA6Y_001510"
     CDS             2937..4061
                     /locus_tag="ACHA6Y_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8987607.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4288..5205
                     /locus_tag="ACHA6Y_001511"
     CDS             4288..5205
                     /locus_tag="ACHA6Y_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8987608.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5198..6031
                     /locus_tag="ACHA6Y_001512"
     CDS             5198..6031
                     /locus_tag="ACHA6Y_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8987609.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6114..7232
                     /locus_tag="ACHA6Y_001513"
     CDS             6114..7232
                     /locus_tag="ACHA6Y_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8987610.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7253..9124
                     /locus_tag="ACHA6Y_001514"
     CDS             7253..9124
                     /locus_tag="ACHA6Y_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM8987611.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            9118..10341
                     /locus_tag="ACHA6Y_001515"
     CDS             9118..10341
                     /locus_tag="ACHA6Y_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8987612.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10427..>12354)
                     /locus_tag="ACHA6Y_001516"
     CDS             complement(10427..>12354)
                     /locus_tag="ACHA6Y_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8987613.1"
                     /translation="LQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKSTLLK
                     IIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMRELE
                     IFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQKID
                     TLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVSHDR
                     YFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLEDFVA
                     RNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDAAIG
                     YDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVTIGY
                     YDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGEKAR
                     VALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRIATK
                     VVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKEQQK
                     LIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQDQET
                     KLTEWEELSLELEEYEN"
BASE COUNT         3968 a         2066 c         2413 g         3907 t
ORIGIN      
        1 cataccaacc aacaacccta tatacttttt catttttact cctcaagtaa aaataactcc
       61 tagaccgcgg ctacccaccg cggtcgtttt tttattcttc aatcaaaatc gctttgatac
      121 ccaaacctgc caagattcgg gcagaagtat aagtattcaa tcctcttctg aaagtaatgt
      181 aaactttttt atcttttggg atttcattga ttcgatctcg taactcattc attggaatat
      241 tttttgttgc cgagatactg ccagaaggtg ctttattagg ttcacgtata tctaaaaaga
      301 acgcttcttt caaatcgttt tcgggtatat cacttgcttt gatcgtttct acgatattcg
      361 tcatttgatt gattgcgaca taaccagcta tattcaacgg gtcacgggta gtagaatacg
      421 gtggagagta aggcaactcc aaatcaggca aatcaaagac tgtcaagttt ccggtgattg
      481 ctacagaaag ttctcccatc cgtttatcga cacctttttc tccaaatgct tgtccaccta
      541 aaatacgacc gctatctgca tcaaaaatca gctttagatt taatcttgta gcttctggat
      601 agaaataggc atggtcaaag ggagtgataa aaacagtttt ataatttgtt atgcctgatg
      661 cttttaattc atgttctgtc attcccgcat aacttgctgt atgatcaaag atttttgcga
      721 cacctgcacc aatatatccg cgatagcgca taggcgtacc atttaaaata tctgccagca
      781 tatgtccttg acgattggcc gcactggaaa gcatactttg gatcggctga ccagtcacta
      841 cactagtcgt ttcgataata tcgccaatcg catagatgtc cggcagattg gtttgtaact
      901 ggtcatcaac aatgatctga cctgtatctg ataattgtat gccggttgct ttcaccactt
      961 cattcttcgg agaaacacca acagcaaaaa ttagcatatc agcagaaagg acggagccat
     1021 ctgataatac tatttctcga cctttatcac ggatctcagt aactcttgtt tctaaatgaa
     1081 cggctaatcc ttctttcagc aatttgtcat aaactaaatt agcaatttct ggatcataag
     1141 ggaaagcgac ttgtggcaat tgatcgatta aagtcacgtc taagccgcga tgcttgaaat
     1201 tctcagctaa ttccagaccc ataacacctg caccaagaat gacgacatgt tgtggatttt
     1261 tttcttcaag gaacgatttg attcgatccg catccgtcac actacggagg acaaagccgt
     1321 tttctgcttc aaaaactccg ggaatatccg gataatctgg tctagcacca gaagagatga
     1381 tcaacttatc gtaatctgtt tttatttctt catttgtgga taggtcttta actgttaatc
     1441 gcttagtaga aggatcgatt gctgttactt cgtgtttagt aaagacatcg atgttgtttt
     1501 ttgttttcaa tatttctggt gtacgttcga tcagcgagtc acggtcagtg atcacacctc
     1561 ctaaataata aggcaaagca caacttgcat aagaaatatt ttctccgcgt tcatagataa
     1621 tgatttcgtg cgcttcattc aatcgacgga aacgagtggc aaatgaagga ccgccagcta
     1681 caccgccaat gacaacaact ttcataaaaa catctccttt tctacgttga tcgtactatg
     1741 ttatggtaaa aaacagcaaa taattgtgcc ggaaaattat tgtttatttt gaatagacag
     1801 aaaatataga gaaataattt gtggataaaa cctaaaaatt tttttctatt ttaaggatag
     1861 ataatacttt gaaggggata accctgtata ttttttaaaa gctgaagaaa aatgatggat
     1921 cgaatgaaat cctaatatat ctgcaatttc tgtataggtt aattcatttt ctagcacgag
     1981 acgttttgct ttttccattt ttagttcttt aaaatagcta ataacacttt ttccattcac
     2041 ttttctaaaa tgttttttta agctggctgg actgataaaa aaaatttggg ctaattgttc
     2101 aagcaaaaaa ttttctgaaa gatgttcttc taaatatcgt atgattggat caactaattc
     2161 atctgaataa agaatatgct tttttttagg aatgactttc tcatttgaag gtattgtcaa
     2221 taaagcaagt tcctgttgat aatgccttgg taaggaagac aattgaattt tttgcagaca
     2281 ataagtaact aataaggaaa gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga
     2341 attgatttcc tgatcatagg aaatagctgg cttactatgt tttagataat caattttgaa
     2401 ctgttcattt ttcagaaaag taatgataga tgttatttct tgatctatag aaaaagattg
     2461 tcccgtaata tgtgctaata attgctcatc aaaataaaac tggataatga taaaagtttg
     2521 tgtttccttt tgagtagaaa agatttggac ttcttgctgt ttatcaatga ataaaaggga
     2581 aggattatct agttctatag atttatgtgc aaattcagca tataaattag cgctttcatt
     2641 tattagtagt cgaaaatagg gagcagcaga taaatgaaga tgggtatcta tggaataggt
     2701 tagtgttttt ttgacaagat tggaggattg gttgtttgtt aacaaaagaa ccactttcct
     2761 ttctcttttt atataaaaag ctttctattt ataattaatg actattataa caaaaatgaa
     2821 aagccaaaac gacaagtaaa atattttttt atataaatat ttgtgttact aattccgtta
     2881 atatacataa gtgaaagcgt tttatataag aacttgaaaa gaaagaaggg atagacatga
     2941 aaaaaagata caaattggtg atagctttgc taaacattac cctattattc agcttaagtg
     3001 catgtggaaa aactaacgaa gaagtaacag caaacatcgc agatccaatg ataaaaaaag
     3061 ccactacatt agaagtatat gtcgcaaacg gcaaactgac aaactatgtt gaaaatgctg
     3121 caaaaatcta taaccgcaaa actggagcaa atattgactt aaaaattgtg aatgtagctt
     3181 cgggaacagc aacagtacaa atgatcaccc caaaattggt aacacacgaa gatatgccgg
     3241 atattatctc catggcagac acttcagcag caggtgtgct ggaaaaattt caagatgctt
     3301 tttatagtgc tgatgaatat ggattttatg acaaatatgg cgatcagttt tatgatcaaa
     3361 aattgaatat tttgcgtcaa caaacgagta atgaaacgat tattccttgg gctagtgatt
     3421 ttaccccagc actttgttat tatcaaccaa aaaaatttgc tgaaataggt gtagattttg
     3481 aagaaatcag ttcttgggaa gaatatatcc aggtggcaaa agcattaaaa gaaaaaactg
     3541 gtagttttgg aattgccttg ccagaatctg gggaccaaga attgtttatc aacatgctag
     3601 cccagcagaa tcaatctttg cttgatgaag aaggaaatat ccgtctgaat actaaagaag
     3661 cgaaacatgc tgcaaagtta atcaaagaaa tgatcgactc agatattgta catttctacg
     3721 gtgcacaaga tgctgagaaa gcatttcaag aatctgcaat gtttgttgct ggcggctggt
     3781 atgctacgaa tatgtctttg aattttcctg atgcatctga caaatggcgc atggctccac
     3841 tgattccttt ttcaaaagaa aatccaggaa aaggtgcagt atcaggagga tcaagttttt
     3901 atgtccctaa agatgcaaaa aatccacaag tggcacaaca atttcttaca tttatgctga
     3961 cagatgaaga atgcttagcc aacgcactgg agctgggcgt ggctacatct aactcattgg
     4021 cctatcaaac agaagcagct gagaaaaagt ttgactacta aggaggacaa aaatactatg
     4081 aactattgaa taaaatcaat gaaaatacat caaatactgt tttaccagcc acttattcag
     4141 atgctacgtc ttatttgact accgctgcat ataactattg gcaaacaggt gattttgaaa
     4201 attcatatct aaaagaagca gaaaattttg ctcataagta cggcgtaaaa ttaactggtg
     4261 attaaaaaat attaggagga cttaacgatg atgcaatctt cgttaccaag caagacaata
     4321 aagaacagca agaaaaaagc aacagatacg tctcataaaa aaagtgctta tggattggtc
     4381 acaccttttg ttattttctt tattgtgacg attttgattc ccatcattat ctcgtttgct
     4441 tttagtttta tgaaaattgg ctactcattt gaatttgttg gatttgacaa ttatctagct
     4501 atcttccgag atccattgtt tattaaatct tacacgaatg ttttgattat catggcagga
     4561 tcaattccaa taacgatgct attagctatt tactttgctg ttttattgaa ttcagcacag
     4621 ttaaaaggaa aaggattttt tagaactgtc tattatattc caaccattac atcaacggtg
     4681 gctgtagctt ccgtattcat gacttttttc aatccgactg ggttattcaa tggatttttg
     4741 aatgcgctag gaattaacgg tgtacagtgg ttaacaaatc ctttttggat acgaatatca
     4801 atgatcatca cgatgatttg gcttagtgta ggatataaca ctattttatt tttagctggt
     4861 cttcaaggga tttctaaaga gatatacgaa agtgctgaaa tagatggagc atcgaaattt
     4921 cggcaattct ttttattaac gatccccatg ctaaagccaa tcattttaat ggccacagtt
     4981 ttagccacta tcaatggctt gggtaatttc aatatcccaa atatattctt tgggacttcc
     5041 aatggaccag aaaattcagc tttggtcgta ggagtcaatt tatataaaac aagttttgaa
     5101 atggttgatt ttggaaaagc ctctgctatt gcctggacga tggtgtttgt atctgttata
     5161 ttatcggcta ttcaatttaa attcggaggt gataaaaatg actaaacaaa agatacaaag
     5221 cagaattttt acgtacgtca ttattgtgtt tggtgcctta atatttttga tgccatattt
     5281 atatatgatt gtagcttcaa cgcaaagtaa tcaagaaatt ttaagtggcg gaatcaattt
     5341 caagtttggg gatatgctat taaaaaactg gcaagccatc atggcgagat ataattatcc
     5401 cttgattatt tggaattcga ttgttattgc tgtaatcagt acagttttag ctacagccag
     5461 ttctacaatg gcgggttatg cattagcaaa gtatcgtttt atggggaata aactgttgtt
     5521 caatacagtt atgctatcta ggatggtgcc tttttttacc acattgattc ctttatttta
     5581 cattatgagt cgtttaggat tatcgaatac ttatgcaggt gtgatcatcc cttcgattgc
     5641 cagtacaact tcagtgttta tgatgcgaca atatagcagt caatttccta cagcactgat
     5701 ggaggctgcc agaatcgatg gtgccaatga gtgggtgatt tttacgaaaa tcgctgtacc
     5761 gattttatca cctacgatta ttactagtgg tttacttatc tttatgggaa gttggaatca
     5821 atatctattt ccattagtca tgctttcaga taaagataaa tatacggttc ccttaattat
     5881 caaaaatatg acggtttcta gtacaggtga gccaattaat tatggcgcaa ttatgctaat
     5941 tttagcaact tcagtgatcc caatggtctt gatttatgtg tgggcgcagt ctaaatttaa
     6001 ggagaatgac attggagctg caaataagta aaaaaaataa cgaatgttaa ccatctagtt
     6061 cgttctagga gcaactccac attcataaaa attaaaagaa tggcgggata tccatgaatg
     6121 aacagacgaa ggaatggtca agaaaaattt tgaatgtagc ttctgaaaag atagatgcgg
     6181 aattagacag aatggggaat aaaattccat atatttctta caacggaaaa tatacagacg
     6241 taataaagga cagaggaatc gactggtgga ccaatggttt ttggagtggt attttatggc
     6301 agctctttca caagacaaaa cgagaaaagt tcaaagaggc agcaatacaa caagaacaat
     6361 tattagaatc tgccctgtac catttttcag acgttcatca tgatgtaggt tttttattct
     6421 taccatcggc tattgcacat tatcaacagt ctcaaaacaa acatagtcga caactagggt
     6481 tacatgcagc tacggtactt gctggacgct ataatatttc agggaactat ttagtagcat
     6541 ggaatgacca taaacctggt tggatgatta ttgatagtat gatgaattta ccattactgt
     6601 attgggcttc gagggaaact ggtgatccga ggttctctaa aattgctcgg cagcatgcag
     6661 atacaacagc taagtatcta gtgaggaaag atggatcaac cgggcatatc gcagaatttg
     6721 atgcagaatc agggaaatat attcggttac ttggcgggca aggatacaat gagaattctt
     6781 gttggagcag aggaaatgca tgggctttat atggattcgc gttaagttat cgttacacaa
     6841 aaaatccgta ctatctagct atagcagaaa agatagcagt aaattttatt cagaaaatcg
     6901 aaaaaactgg ttatattcct ctcattgatt ttgctgtaaa agattcgaat attgatacca
     6961 gtgctggagt atgcgctgca tgcggtcttc tggaattagc aggatatgtt tcctctgtac
     7021 gatcaaaaat gtatcatgaa gctgctatca atatactcca aaagacagaa aagcactatg
     7081 caaattggaa tatggatgaa gatgggatca ttggaggcgg agcagaagca taccatagag
     7141 aactgactta taatgtgcca ctgatctatt ctgattattt ttttattgaa ggcttacttc
     7201 gattagaaca gtcggaatta gctatttggt agtaaaaaga ggagaaaata caatgaaaca
     7261 tccagtacca ctattaagga ccagacagga ttactttgac ttatttaaaa agataaatca
     7321 accactccag ccgttttatc gagcaagtgg cgcacaaatt gattatatac atcaaggggt
     7381 aggatatgga aataaaattg caggaatgga aggattctct cgggttttat ggggagcggg
     7441 gcccgcaatt gaccaattag atgaacgatg gttgaaatta attctatcag gaataaaagc
     7501 tggaacagat ccttcccatc ccgattattg gggagaaata catgaaagag accagcgtat
     7561 ggtagaaatg cccgcaatgt tattagcgtt atatcatcat gaacaattat tatggaaaaa
     7621 attagataca ggagaaaaaa gccagattat tcattggttc tcgcaaattt ttgaatatga
     7681 atgtgcagat ggtaattggc aattttttaa gataattgtc ggaacaatta tccaaaaatt
     7741 tggttatcca gttaaagaat cagcactgaa agaagcattt gataatatag aaaattgtta
     7801 tcttcaaaat ggctggtatc gagacagttc aagaggtcgc caagattatt ataatccttt
     7861 tgcatttcat tattatggtt tgatttatag cgtactagaa ccagaaaagc ccattagcca
     7921 gatatataag aaccgagcta ttgatttttc tagagactat attcattttt ttgcggaaga
     7981 tggtgcaaat gtgccttttg gtcgaagcat gatttatcgt tttgcagttt caagcttttg
     8041 gtcagctatg ttgtgggccg atcttttgcc ttttaaatta ggtgaaatca aggggttagt
     8101 caatcggaat ataagatggt ggatgcaaaa agaaatattt gatgaaaaag gtatcctgaa
     8161 tattggttat acctaccctc aattgacatt gactgaaccc tataatagta cgttatctcc
     8221 attatggctg aataagattt ttttattgtt ggcgctacct gaagaccatg agtattggac
     8281 ggctaaagaa atgcctatgc ctattcgtga aggaactaaa cttctttcag aagccaattt
     8341 gctcatacaa catgataatg gacatacatt ttttttgaat gcgggacaat tagggccaaa
     8401 tttccataca ttgaccaatg aaaaatattt aaaatttgct tattctagtc aatttggttt
     8461 ttcgataccg cgagcgaatc aattgaaagc agaaatggca atggattcaa tgctagggat
     8521 tcagcgagcg gacacatata tcacgaccag ttttaagaat gagagcatca aaacctatgg
     8581 acagttcatc gttcgaaata atgtacgaga cattgtatgt aagaaagagg tacttgcctc
     8641 tacatgggaa cctactttac aggcaaaaat cagaacttgg cttattcctt ttgaaggctg
     8701 gcagattcgc gtacacaaag tagaattaga tacagaatat gttctatatg agacagggtt
     8761 tgctattgaa tgttctcctg agaaagaagg aataatcata gaagaagaca aggaaaacta
     8821 tagggtgagt gaagctggct tttcaggtat tatctgttta agtgacgata ctattaaacg
     8881 aaaatccaca gctattatgg gtttgcccaa tacaaacttg atgacatggg aaaacacttt
     8941 tattcctgga ttagagggta catttggaaa aggaacccat tggctcggaa caggagtggt
     9001 tgctcatcaa gatcgggatt atagtttaga agtatggaac aatcgtccca aaattgattt
     9061 tgatgggaca accgggctaa ctatccaaaa taaaaacaat aaaaaaagac taaaattatg
     9121 ctaaaggctg gtggggaaat gcttgataaa gtgatgtttg aagagataat acatccagaa
     9181 aaatggataa atgaatgtta taaagaatta atggaaaaag aatggaataa tgagatcgag
     9241 cgttgtcgca gacacacgga gaggttaatc aaacgtatca gtaacagtca gtatcaattc
     9301 ccaagcgagt gtcctataaa aggacgttat ccattaagag acaatattgg ttggacagaa
     9361 ggattttgga caggaattaa ttggttgtta tttgagaaga cgggtgatcc tttatttcaa
     9421 caaacagctg aaaaagcaga tatagatttt atccacagga ttgactgtca aattatcact
     9481 aatcatcacg atcttggttt tttgtattct ttatcaaccg tagcagatta taaaataacg
     9541 ggaaatcgaa aaatgaagga agcttcaatt gatgctgcag aaacattact tcagcgtttt
     9601 tgggagaagg caggagttat acaagcttgg ggagatccta gtgatccttc acaacaaggt
     9661 agaatgatca tggattgttg catgaatctt ccattattgt attgggcgag tgaagaaact
     9721 ggaaatcaaa aatataaaaa agctgctcag gctcatattc aaaatgcagc agattattta
     9781 gtaagagaag atggttcaac ttatcataca ttttatatgg atgtcaaaga tggaaaacca
     9841 agatatggaa gtacgcatca aggttatagt gataattcct gttgggctag aggccaagca
     9901 tgggggattt acggttttgc attaagttat cgttatttga aagaacaaaa atattttgat
     9961 ttaagcaaaa tattagccaa ttattttatg aatcggttgc cagaagatac catttgttat
    10021 tgggatttga tctttgatga ttctagcaga gaagaaaaag atacatctag tatggtgatc
    10081 atggtttgtg gattattaga attaatagaa tcaggtctat tagacaaaac agaaaagaac
    10141 ttataccagt ctgctgtttg gaaaattatg cgacaattga aagaaagata tgctacaacg
    10201 gaagaagaag atggcttttt aacacagtct gtttataata agcccaaaaa taatggggta
    10261 aatacacctt gtatttgggg tgattactat tatttagaag ctttagtgag aatgactagt
    10321 aattggtctt cttattggtg aaaaaggtaa atatggaatt tttgtttgat aaaataaaaa
    10381 aagaaaaacc aaaacagcat gaatggtttc tgttttggtt ttttcgttaa ttttcatatt
    10441 cttctaattc caagcttagt tcttcccatt ccgtcaattt tgtttcttga tcttgtctag
    10501 cagcttctaa atcttggttc agctcattga gttttacgtg atcttccagg ttttctggcg
    10561 cgaccatttc ttgttccaac tgatcgatcc attcatcgat ccgcgccatt tcttcttcaa
    10621 cctgagtaat ttttcgttga aggctacgga taagcttttg ctgttctttg ctttggtaat
    10681 aatcagattt gttcgtggga gcaggagcag attctggctc atcttgtgcc aacaattcgg
    10741 cgatttcttc ttcttcctgc tttttctcta aataataatc atagtctcct agatagactt
    10801 tgctgccatt ttcagagagt tcgacgactt ttgtcgcgat ccgattgatg aaatatcggt
    10861 catgggaaac aaaaagcagt gtgccgtcat aatcgatcaa tgcattttct aatacttctt
    10921 tgttgtcaat atctaaatgg tttgttggtt catccaaaat caaaaaattt tctttgttca
    10981 tcgctaactt ggctaaagca acccgtgctt tttcaccgcc acttagcaga gggattggtt
    11041 tttctacatc ttcgccagaa aataagaaag cacctaaagc acttcggatc tctttttctg
    11101 gagtggtagg atgctcatcc cacaattctg ctaggatcgt cttgtttgta tgcagatcag
    11161 cttgaccttg atcgtaatag ccgatggtga catttgtccc tagcgtttct tttccacgga
    11221 taaaagggat ctggtgaatg attgatttca gcaacgtcga tttcccgata ccattcggac
    11281 ctactagagc aatcgcttct tgacgacgga tatctagatt gattggttca gataaggtga
    11341 cattatcata tccgatagcg gcatcttcga tttgcaagac tacatttcca gaaggctttt
    11401 ctatactgaa tagaaagttt gcttgttttt catccccttg aggacgatcc agccggtcca
    11461 ttttttctaa tgtctttctg cggctttgcg ctcgttttgt ggtagaggcg cgaacaaggt
    11521 tccgagcaac gaagtcttct aatttatgga tttctgtttg ctgcttttca taagccttcc
    11581 attcacttgc taactgttct gctttcaaat ctagatattt cgaatagtta cctttgtaat
    11641 ggcgcatttt tcttctgctt aattcataga cttcattgac tactttatct aaaaaataac
    11701 ggtcatggga aacaatcaat aatgcaccag tatagctttg cagataattc tcaagccagg
    11761 aaagtgtctc gatatctaaa tggttcgttg gttcatctag aatcaaaatg tcagggtgtt
    11821 gcaaaagcat gcgagctaaa gctaaacgtg tgcgctgtcc gcctgataaa gtatcgattt
    11881 tctgatcata aaatgaagaa tcgaatttaa acccatgcaa tacggaacga atttcatttt
    11941 cataaccata gccgtttcga tctgaaaaat cgtgttgcag ccggtcgtat tcttttaaat
    12001 aagaactata agcaggtgtg tctggcgtac cttcgctaat gaaaatctct agctcgcgca
    12061 tccgctcttc catttgtcta acttgttcaa aagctttcag catctcgttc caaactgttt
    12121 cattggaatc aagtcctgtg tcctgagcga ggtatccgag agtcgctgtt ttatttttag
    12181 cgatctttcc ttcatcaggt gcttcaattc cagcaatgat ctttaataaa gtcgattttc
    12241 cagcgccatt tctgccaact aaagcgacac gagcgcctgt actgatttct aaatgtatgt
    12301 tttcaaataa gacttctgca ccaaaatggc gagcaatctg attggcttgt aata
//
LOCUS       DAYYHX010000074        12100 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000074 DAYYHX010000000
VERSION     DAYYHX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.075"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..550)
                     /gene="frr"
                     /locus_tag="ACHA6Y_001517"
     CDS             complement(<1..550)
                     /gene="frr"
                     /locus_tag="ACHA6Y_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM8987614.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELL"
     gene            complement(553..1275)
                     /gene="pyrH"
                     /locus_tag="ACHA6Y_001518"
     CDS             complement(553..1275)
                     /gene="pyrH"
                     /locus_tag="ACHA6Y_001518"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM8987615.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1411..2292)
                     /gene="tsf"
                     /locus_tag="ACHA6Y_001519"
     CDS             complement(1411..2292)
                     /gene="tsf"
                     /locus_tag="ACHA6Y_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM8987616.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2391..3173)
                     /gene="rpsB"
                     /locus_tag="ACHA6Y_001520"
     CDS             complement(2391..3173)
                     /gene="rpsB"
                     /locus_tag="ACHA6Y_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM8987617.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3532..4011
                     /locus_tag="ACHA6Y_001521"
     CDS             3532..4011
                     /locus_tag="ACHA6Y_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8987618.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4228..5319
                     /gene="phnW"
                     /locus_tag="ACHA6Y_001522"
     CDS             4228..5319
                     /gene="phnW"
                     /locus_tag="ACHA6Y_001522"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFM8987619.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5312..5440
                     /locus_tag="ACHA6Y_001523"
     CDS             5312..5440
                     /locus_tag="ACHA6Y_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM8987620.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5444..5989
                     /locus_tag="ACHA6Y_001524"
     CDS             5444..5989
                     /locus_tag="ACHA6Y_001524"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM8987621.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6443..8134)
                     /gene="argS"
                     /locus_tag="ACHA6Y_001525"
     CDS             complement(6443..8134)
                     /gene="argS"
                     /locus_tag="ACHA6Y_001525"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM8987622.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8554..9501)
                     /gene="arcC"
                     /locus_tag="ACHA6Y_001526"
     CDS             complement(8554..9501)
                     /gene="arcC"
                     /locus_tag="ACHA6Y_001526"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM8987623.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9616..10635)
                     /gene="argF"
                     /locus_tag="ACHA6Y_001527"
     CDS             complement(9616..10635)
                     /gene="argF"
                     /locus_tag="ACHA6Y_001527"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM8987624.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10726..11955)
                     /gene="arcA"
                     /locus_tag="ACHA6Y_001528"
     CDS             complement(10726..11955)
                     /gene="arcA"
                     /locus_tag="ACHA6Y_001528"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM8987625.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3304 a         2517 c         2191 g         4088 t
ORIGIN      
        1 ccaataattc tttttcttta tcagcagcga tcttatctac ttctttgatg ctgttatctg
       61 tcaatgtttg tacgtctttt tctaatccgc gcaaatcatc ttcggtaata tcaccatttt
      121 tttgttgttt tttatattca tcgatcgcat cacgacggat gttacgaatc gcgatttttg
      181 cattttctgc ttctttttta acatctttag ctaattcttt tctgcgttct tctgtcagtt
      241 gcgggataac taagcgaatg acatttccat cattggttgg actgattccg atatcacttg
      301 ccataatagc tttttcaaca tcttgaagaa tgctcttgtc aaaaggtgtg atcattaaaa
      361 cacgtgcttc tggaatttgg attgatgcca tttggttcaa tggtgttggc gcaccgtagt
      421 agttcacagt aatacggtcc aaaaggctag cgttcgcacg gcctgcgcgg atttgcccaa
      481 gttcacgttg aagattttgt gctgcttttt tcattttttc ttttgcttct gtcataattg
      541 catctgccat ttttatttcc cccttacagt tgttccgata ttttcgccta agattgcacg
      601 gcgaatattt ccgtgttcat ttaaattgaa gacaagcagt ggaatatcat tatccatgct
      661 tagtgaactt gcagttgaat ccatgacttg taatcctttg gcaataactt ccagatgagt
      721 cagttcttca aattttactg catttgcatc tactttcgga tcagcagaat aaacaccgtc
      781 tacattgttt ttagccatca ggataacatc tgcgccgatt tctgccgcgc gtaatgctgc
      841 tgttgtgtct gtcgagaagt acgggttgcc ggtaccgcct gcaaagataa cgatccgtcc
      901 tttttctaaa tgacgttctg ctttacggcg aatatatggc tctgcgattt gacgcatttc
      961 aatcgaagtt tgcacgcgtg tcggtacacc cacgttttcc aacgtatcct gtaaagctaa
     1021 ggcattcata actgtagcta acatacccat gtaatcagct tgagcacgtt ccatgcccat
     1081 ttgtgctccg atttgtccgc gccagatatt tccgccgccc acaacgattg ccatttcaac
     1141 gcccagctcg tgaacttctt tgatttcttg gatgatttct ttgattactg gtggtttgat
     1201 tccaaatcct gcgtccccag ctaaagcttc tccactcaat tttaagacta cacgctgata
     1261 tttcggtgtg accatgttca ttcctccaat attttctatt gccattttac catacttaga
     1321 tgatttcgac actctaagac tggtttttct tgttttgctt aaaaaaaggg agcgcacaaa
     1381 ccgtgcgttc ccgtctgtta ttcaacagaa ttattttttc acttggctca taacttcttc
     1441 tacaaagtta tcttcgcgtt tttcgatacc ttcgccaact tcgaaacgta cgaatgtttt
     1501 aacagttgcg ccttttgatg caacgtattt ttcaacagtc atatcaggat ctttaacgaa
     1561 tggttggtcc accaaagcga tttctgcttt gaatttcttc aagcgtcctt ctaccatttt
     1621 ttcaacgata ttcgctggtt tgccttcgtt caatgcttgt tctgtaagaa cagttttttc
     1681 atgttctaat tcagcttctg gaatttgtga ttcgtttaca taacgaggat tgatagctgc
     1741 tacgtgcata gctacgtctc ttgcaactgt ttcgtcagtt gttccatcta gaacagtcaa
     1801 tacggcaata cgtccgccca tatgcaagta tccaccaaat gcagcattgt cgtctttttc
     1861 tacaacttca aagcgacgga agctgatttt ttctccgata acttgagttg cttcgatcaa
     1921 gtcagattca attgtacctt tatctgtttt gattttcatt gcagcttcca tgtctgctgg
     1981 tttgttttca gcaacaagtt ctgcaatttc ttttactaaa tcttggaaca tttcgttttt
     2041 agaaacaaag tctgtttctg agtttacttc aacgattgct gcagtgttac ctttcacagc
     2101 aactgaagcc aatccttcag ctgcaatgcg gtcatttttc ttcgcagcct ttgccattcc
     2161 tttttcacgt aaaagatcaa ctgctttttc catgtcgcct tctacttcaa ctaatgcttt
     2221 tttcgcatcc atcattccta cgccagtcat ttcgcgtagt tcttttacca ttttagctgt
     2281 aacgtctgcc atgtttaact cctcctgtgt gtttttcttt ttaaaaaaag ctgcttcaaa
     2341 ggagagtgag gcttgtcgcc cagcctttga aacagctcca tcattcaaaa ttattctgct
     2401 gaagaattgt cgccttcaac aacatctacg atttcttcga ttgatgttgc tgtgttttct
     2461 tctgtgaaat cttcttcaac tacttgatct tcaccttggt ttccttcgat gaaagcatca
     2521 gccatttttg aagtaatcaa tttaacggca cgaattgcat cgtcgtttga agggattact
     2581 acgtcgattt catctggatc gcagtttgta tcaaccatag ctacgattgg gatatttaat
     2641 ttgtgtgctt cttgaacagc aatacgttct ttacgtgggt caacgatgta cattacatct
     2701 gggattcttg gcatatcagc gataccgcct aagaattttt caagacgttc acgttctttg
     2761 ttcaaaccag caacttcttt tttaggtaga acttcaaaag ttccatcttc ttccattgca
     2821 ttgatttgtt tcaaacggct gatacgtttt tggatagtat cccagtttgt caatgttccg
     2881 cctaaccaac gatggttaac gaagtattgt cctgcacgag ttgcttcttc tttgattgct
     2941 tcttgtgctt gtttttttgt acctacgaat aatgctacgc cgccttcttc agcaacgctc
     3001 ttcatgtaat cataagctgc atctactaat ttaactgttt tttgcaagtc aatgatgtag
     3061 attccgtttc tttctgtgaa gatatatttc ttcatttttg gattccagcg acgagtttgg
     3121 tgaccaaagt gtacgccggc ttctagtaat tgtttcatag aaattactgc catgtttgtt
     3181 tcctccgatt tggttttatt tttcctcttc ttatcttcta ccttgccggc aaactttttc
     3241 aagcacctac cgacaatcca ataagaatgt ggatttgctg atgcataaag caccctcagc
     3301 tattatacag acaaaagatc cgtatcgcaa gagcaaatat acttttctgc tcaacattca
     3361 atagtttacc tgaaagataa aaagaattca agaaaaacag tagatttctc tttgtttctt
     3421 tattatcctt tttccataaa ataattttag gtataaaaga cgaatattat tgggcaatcc
     3481 ttcttttttt tgttatgata catgtgaaat ctttcgtaaa ggagaaagaa aatgcgcaaa
     3541 gctcaaagac atttgctgat taaacaagtc attgaagaat tcccgatccg tacgcaagaa
     3601 gaactattaa acaaacttgg gcattatggc gttaccgcca cgcaggcaac gatttctcga
     3661 gatatccgtg atctaaaaat cgtgaaggca cctgatgaaa atggcgtttc ccgcttcgta
     3721 ttgtttcgcg aaaaaggaac tcctgagact aggactgagg aagaggatcg gctggttcaa
     3781 atgattgaag acattgtatt gaaagtcgaa cgagtccact ttttgacgat cgtcaatacc
     3841 ttgccagaca atgctcattt atttgcatct gttttagatg agatcaaacc tccctctatt
     3901 gtcagtacaa ttgctggatt cgatacgatc atggtcatct cggaaaatga ggaggacgca
     3961 aaacgagtag aagaatttct tcaccatcca actgaagtat cactttttta atcagagcac
     4021 gtattcctaa tatggaatgc gtgttttttt attttcaata ttttccttat tgaataaacc
     4081 tttacactct ttacaaaaat acatcatttt ttattcaaat ttttcaacac cttgattgaa
     4141 tcacaataat catcatttat attggatgtt aacaagagca gcttcttgct ctttgtgtat
     4201 ttcaaaaaag aaatgaaagg aaatcatatg tataaattat taacttctgg accattgacg
     4261 acttctgaaa cagtgaaaaa tgctatgctg atcgatcatt gtacttggga tcaagaatac
     4321 aaagaaatta cacaatggat aagaaaaaaa ttattggatc ttgctcaagt atcggaagga
     4381 atatacacaa cggtcttaat gcaaggtagc agctccttcg gggtagaatc ggtattaagt
     4441 tctgtgatca ggccagaaga tactgtactg atttgttcaa acggcgcata tagacaacga
     4501 atgatcaaca tgtgcgaatg tctgaaactg aactataccg tttatgaggt atcggaacat
     4561 caaatcctcc aagctcatgt gattacacag ctgattgaag agaatccgtc tatctccgca
     4621 ctagcaatgg tccatagcga gactacttcc ggtattttga ataatttgga agagattgct
     4681 gctgtcacac aagaaagaga tcttttgttt atagtggacg cgatgtcaag ctttggtggg
     4741 ataccgattt gtgtagccga actagggatt gattttttga tttccagctc aaacaaatgt
     4801 attcaaggtg ttccaggctt ttcttttgta cttgctaata aagcggaact ggaaaaacga
     4861 aaaaatgtta gccgaagttt gtcacttgat ctatatgacc agtaccgcgt gatggaaaac
     4921 gagaatggca aatggcgttt cacttcccct acccatacgg ttttggcttt tcaacaagcg
     4981 ctgaaggaac tagaggcaga aggaggaatc gaaactcgat attcacgtta tctcgccaat
     5041 aatcatgtgc tacgacaaaa aatgacagag atggggttcc agcctttcat tcaagaatat
     5101 caggggccat tcatcacctc ttttctttac cctaaggact ggcgatttga ttttaatcat
     5161 ttttatcagt atttaaagca aaatggattt gtgatctatc ccgggaaaat cagcgatcat
     5221 gaatgttttc gtatcggaaa tattggcgag atctatccgg aagatatttt ggaactgaca
     5281 gcaattatca aagactataa ggagaacgtt aatgattaaa actgtaattt ttgattgggc
     5341 aggaacaact gtggactttg gctgtatggc tcctgtccac gcttttagaa atgcttttct
     5401 agaaaaagga atccaactga ctgacaaaga aatccgctaa ccgatgggaa aattaaaatg
     5461 ggatcatatc caacagcttc tttccctccc ttctgtcaaa aaacaatgga tagctattta
     5521 tggacaacta cctcaggaaa aagaggtgga ggaattgtat caacaatttg aacattatct
     5581 gtttaaagat ttggtcaagc attctacctt gaaacccgac acattggaaa cgatcgaacg
     5641 gttgagaaac gctgggatca atattggttc cacgaatgga tttaatgctc ctatgatgac
     5701 aatcgtagca gaaaccgcaa aaaaagaaag gtattcacca gacttcatgg caacttttga
     5761 agacgtcgaa gggtatggtc gaccgtttcc ttatatgatc cataaaaaca tgcagtactt
     5821 caaaaacaag tcggttgatg aagtaatcaa agtcggagat acggtctcag atatccagga
     5881 agggaaaaat gccggagtgt tgacagtagg tgtaattgaa ggtagttcgc taatggggat
     5941 ttcttatgat gagtaccaaa aattagagat ggggaaaaag atcgtttaaa acagtctgtt
     6001 gctcttagat tccaacaagc cggcgctgat atttgtgtaa acaatttaaa agagttaaca
     6061 gaatacattt tgtacgctga catgaatcca gaatgacttt tatacaaaat aagaggttgt
     6121 gaaaaagctg tcctgtatca agtaataaaa acagccaagc aaaaatagct cctcatattt
     6181 ttcgtttggt taggcttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
     6241 aaaaagggag ctgtgacacg acttttgtca cagtcccctt atttgcaaac aaacagtgtt
     6301 taagagacaa cctctccact aaacggtgtt cattcagctg actctcggta tttgctcaaa
     6361 aaacgataaa atttgaaaag ctattttctc ttttgtttct ctaatactcg gatcagaacg
     6421 ctgatttcac attctctttt tcttacatct tatctggtgc atgaagtcct agcaaacgaa
     6481 ggtcttcttt caataataca gtcactgcat aaactagagc taagcgtgct tctttttgtt
     6541 catcatcagc taaaatcttc gtatgagcat agtatttgtt gaatgcttgt gctaatttga
     6601 ttgcatgttt cgcaatcact gacggttcgt atttttcccc agcagacaag accgtttcag
     6661 ggtatttttg aactaattta accacttccc agctggtatc gtcattcaat gcatagtctg
     6721 ctgtttctga agggacaaat cctgcttttt ccaacaggct gactgcacga gcatgtgtat
     6781 attgcacata aggtccagtt tctccttcaa atcgaacaac ttcttccagg ttaaagtcaa
     6841 atgtattcag tcgatcgttt ttcaaatcgt ggaaaatgac tgccccaacc ccaacttgtt
     6901 tagctacagc atctttgttt tccaaatcag ggtttttctc gctgatttgt tctttagcag
     6961 attggattgc ttcatttaat acttcttcta ataagacgat ctttccttta cgagttgata
     7021 attttttacc accttgcgtg atcaaaccaa atggaatatg gtgcatgtcg tcagaccagt
     7081 caaagcccat ttccttcaag acagctttca actgtttaaa atgatagctt tgttcattcc
     7141 ctactacata tagagattgt ttgaagtcgt aagtacgttt acggtacaat gcagcagcta
     7201 agtcacgtgt aatgtatagt gtcgcaccgt ctgacttctt aatcaacgct ggattcaggt
     7261 cataagcaga taggtcaacg atctctgcac ctttatcctc gttcagcaaa tgtttttctt
     7321 ctaaaagttt gacgatttca tccatcttgt cattgtagaa agcttcaccg ttcaacgaat
     7381 cgaaacgtac ttccagcaga tcatagattt tattgaattc tttcatcgat tcatcacgga
     7441 accattgcca taattgaata gcctctttat cgccttcttc cagacgtttg aaccaagcac
     7501 gagcttcttc atttaattct ggttctgttt cagctacttc atggaattgg acgtataaac
     7561 gaagcaattc attgattggt tctgctttaa cagcttcttc tgtaccccat tttttgtaag
     7621 cgacgatcag ttttccaaac tgtgttcccc agtcacctaa atggttgatg cggattggct
     7681 gataaccgat tttttccata atgaaaccga tcgaattacc gataactgtc gaacgcaaat
     7741 gtcccattga gattggtttg gcaatattag gagaagacat gtcgattggt actgttcctt
     7801 gattcccgat gttgctatcc ccataatgtt ctttttcttt cactactgtt tgcaaaactt
     7861 ttttgctgat aagttcttta ttcatgaaaa agttcaagta aggtccgaca acttcgattt
     7921 tttcaaagtt ggcagaatcg attttttctg ctaaatctgc agcgatttgc tgtggcgctt
     7981 ttcggtaaac ttttgctaat gaaaaggctg gaaatgcaac atctccgtgt tcagctgatt
     8041 ttggattttc tagtaattgc tcgacttgtt ccagtgttaa atcatctttg acagcgtcat
     8101 agacagcttt tgcgacaaga tctttattat tcatctttat tcctcctaaa gtgttccctt
     8161 gattgttaaa caaaaaaaat ctctagcgac ggcatactta gtatgccatc actagagacg
     8221 agatttcccg cggtaccact ccaattgtcc taaaaaagga ccagctccat ctgatatcgg
     8281 tagacgaccg tctgtttaaa acagaattct ccaagtgcgc ttcgttgtat cttcttcatt
     8341 cggcttccac cacttccgaa ttcgctagcc aagaatgata caagtactct cttgttcaac
     8401 aaatcttagt cttattaaac tttcttgaat tatatcagaa aaaaaaacga aaggctagtc
     8461 gtttcataaa aaaacaatcc agatggccta aaggagaaga aataccacct ggatagctat
     8521 aaatcatttc gtaaatacga aagacttaac aaattaatca gctacgataa tcgttccttc
     8581 ttcagaagct aaaagattct cgatattttc taaagaagtg atgattgcct tagcgtttgg
     8641 ttttgcttca acaaatgcaa tagctgcttc tactttcgga agcatgcttc ctggagcgaa
     8701 ttgcttttca tcaatgtatt gtttcatttc tgaaacagta acagtttcta atttcttttg
     8761 atctggtttt tggtaattga catacacatt gtctactcct gtcaatacga tcagcaaatc
     8821 cgcatcgatg atttcggcta atttttctga agcaaaatct ttatcgatta cggcttctcg
     8881 tccttctaat ccagtagctg tttctacaac aggaatccca ccgccaccaa cagagactgt
     8941 gataactcct tgatcgacca gcgtttcgat gacacgtgct tctttaatcg agatgggttt
     9001 aggggaagcg actacttttc tccatccacg tccagcatct tctttgaatg tcacagtgga
     9061 gtcagcattc atttgttctt tggcttcttc ttccgtgtag aatggaccga ctggtttact
     9121 tggatttttg aatgaaggat cattttcatc cacaataact tgagtgacta aagaaaccac
     9181 atctttatcg atcccttttt ccttcagtac ttcgcccatg gcgttttgta accagtaacc
     9241 gatagatcct tctgtcattg caacacatgt atctaaaggc attgccggtg ttttctcaga
     9301 atcagcagct tgctgctgaa tcaataggtt accgacttgc ggaccatttc cgtgggagat
     9361 gatcaattca tctccttgtt cgataaattt cactaaatac tttgctgttt ccattaacgc
     9421 ttcttgttgg gcttttgcac ttgcatccgt agacaagatg gcattgcccc ctaaagcgac
     9481 aactactttt cgtttggcca tttcattcgt cctttcttca gttctaccga attttctaga
     9541 taacaaaacg agccagcgtt ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga
     9601 tgaatgtggt tatgcttaaa cgcgagggat aaacaagtta cctaatgttg ctgccatgat
     9661 cgctttgatt gagtgcatac gattttctgc ttggtcaaat tgacgagcat atttgctgcg
     9721 gaagacttca tctgttactt ccatttcagt gatgccaaag cgttctttca tttgttcacc
     9781 gtaaacagtg tttgtatcat ggaaagctgg caagcagtgc aagaagatca aacgatctgt
     9841 gttgtgtgtt ttttctacca tttccatatt gatttggtaa ggtttcaaca atttgatacg
     9901 ttcttcgaat ttatcttctt cacccattga tacccataca tcagaataaa ggacgtctgc
     9961 tccgtctaca cctttatcaa catcgtcggt aatcatcaat tgtgcaccgg atttttcagc
    10021 aaagccttct gccattttga cgatttcttc ttcaggttgt aattcttttg gtgcaacgat
    10081 acgcatattc gctcctaaaa ttgcacctgt gactaataaa gagttagcca tattgtttcg
    10141 accatctccg cagtaagcaa cagtgatacc ttctactgtt ccaaagtttt cttggatagt
    10201 taaaaagtca gcgatcattt gtgttggatg ccattcatca gtcaagccat tccaaactgg
    10261 aacaccagag aattctgcta gttcctctac cattttttga ctgaatccac ggaattcgat
    10321 tccgtcaaac atacgtccta gtactttagc tgtatcttct gtggattctt ttttacctaa
    10381 ttggatatca tttgcaccta gatattcagg atgtgcacct agatcgatcg ctgcagtagt
    10441 aaatgcagaa cgagtacgtg tagatgtttt ttcaaataaa agcgcaatat tttttccttc
    10501 tagatagtga tgtgggatac cacgtttttt caagtctttc aagtgttctg aaaaatcgat
    10561 caaatattgt aattcttctt ttgtaaagtc tttttctgct aataagcttc ttccttggaa
    10621 tactgattct ttcatattaa acattctcct ttagattgtt tttatttaag attaaagagg
    10681 aaatcccgac ggcagctgac agtaagactg ccgtcaggaa acaaattact tcaagtcttc
    10741 acggacaagt ggttggctca tgcagcgtgg accaccacga cctcgtgaca attcacttga
    10801 attgatttct aacactttga tgccatagct acgaagcaat tcgttcgata cataatttcg
    10861 gttataagtg actacaacac ctggagcgat tgccaatgtg tttgatccat cattccattg
    10921 ttctcgaggt gcaacgatct catctccatt accagttggg atcagtacaa gatcatctaa
    10981 tccaagagca gctttcatcg tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc
    11041 accatctggt tcgattgtaa agacatccat cttaccgtct ttactttgaa ttgctggatg
    11101 gatagtgaat ttatcgtgat ctaccattgt aaatacggta tccaaatgca tcattgcacg
    11161 gttgttcgga attttgattg ccaacacttt ttcaaatcct gaattttttg cgaataggtg
    11221 gcgtgctaat ttttctaaag ctttcgcatt agttcgttga gaaatcccaa cagctactac
    11281 tttatcgctt aagatcaatt cgtctccgcc ttcgatatga tctggatttt cacgatccaa
    11341 ccatacttcg attcctttat ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa
    11401 catcgattca cgacgacgtg cttcaaaagt catcttattg actgtcatcc catttcccat
    11461 tgatgcggaa gggtcgcgtg taaagtacaa gtttggcatt gggtccatgt aaaatggata
    11521 gtcatcgtct gctgacagat catatagact gctagaacgt acttcgatat cttttgtacg
    11581 aaccccagcc atgattttat ctaccatttc ttgcgtttcc attgatagaa ggaactcatg
    11641 aagaccctct cttacagcat tcgatgcaat atgagattca tccaacattt tattcaagaa
    11701 ttgttctttg acattgcctg cgtcaatcgc ttctgccgct aatttttcca agtaaagagt
    11761 ctcgacgcct tcattttgca atgtcttcgc aaagttgtca tgttcttctt gagcaatcgg
    11821 aagatatgga atatcatcga acaacagacg atccataata tctggtgtca aattttccac
    11881 ttcttgtcca ggccgtttca gtaaaactgt ctttaatttc cctatttcag agaaaacgtg
    11941 aataggttta tccatgttgt gttgcctcct tctttttgat tacatgtata ggatatcctc
    12001 cttttgtaat cactttcata attttgtctc attcaaaatg agacattcct cacattaata
    12061 ttcgataatt aaataattaa acgtttttct atttttaagc
//
LOCUS       DAYYHX010000075        11940 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000075 DAYYHX010000000
VERSION     DAYYHX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.076"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..264
                     /gene="yaaA"
                     /locus_tag="ACHA6Y_001529"
     CDS             22..264
                     /gene="yaaA"
                     /locus_tag="ACHA6Y_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM8987626.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            251..1375
                     /gene="recF"
                     /locus_tag="ACHA6Y_001530"
     CDS             251..1375
                     /gene="recF"
                     /locus_tag="ACHA6Y_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM8987627.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1372..3318
                     /gene="gyrB"
                     /locus_tag="ACHA6Y_001531"
     CDS             1372..3318
                     /gene="gyrB"
                     /locus_tag="ACHA6Y_001531"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM8987628.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3338..5809
                     /gene="gyrA"
                     /locus_tag="ACHA6Y_001532"
     CDS             3338..5809
                     /gene="gyrA"
                     /locus_tag="ACHA6Y_001532"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM8987629.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6045..6344
                     /gene="rpsF"
                     /locus_tag="ACHA6Y_001533"
     CDS             6045..6344
                     /gene="rpsF"
                     /locus_tag="ACHA6Y_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM8987630.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6394..6906
                     /gene="ssb"
                     /locus_tag="ACHA6Y_001534"
     CDS             6394..6906
                     /gene="ssb"
                     /locus_tag="ACHA6Y_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8987631.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6932..7168
                     /gene="rpsR"
                     /locus_tag="ACHA6Y_001535"
     CDS             6932..7168
                     /gene="rpsR"
                     /locus_tag="ACHA6Y_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM8987632.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7311..9284
                     /locus_tag="ACHA6Y_001536"
     CDS             7311..9284
                     /locus_tag="ACHA6Y_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM8987633.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9290..9742
                     /gene="rplI"
                     /locus_tag="ACHA6Y_001537"
     CDS             9290..9742
                     /gene="rplI"
                     /locus_tag="ACHA6Y_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM8987634.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10016..11383
                     /gene="dnaB"
                     /locus_tag="ACHA6Y_001538"
     CDS             10016..11383
                     /gene="dnaB"
                     /locus_tag="ACHA6Y_001538"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM8987635.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         3984 a         2172 c         2648 g         3136 t
ORIGIN      
        1 aatgaaaatg agggatgggc attgaaacaa caagtcattc taaatacgga attcatgacg
       61 ttgggccaaa tgttgaaaga agtcacagtt atcggtagtg gcggacaagc aaaatggtat
      121 ttggcagaaa acactgtgtt agtcgatggc gaaccagaaa atcgtcgtgg acgcaaactt
      181 tatcccggca tgatggttga agttccagaa gtaggaactt tttttatggt aaaaaaagga
      241 aacgacgata atgaggctga ataagctgta tttaaaaaat tatcgaaatt acgaagagct
      301 gaacatcgaa ttttccaaga acctcgtcat ctttttagga gaaaatgcgc aaggaaaaac
      361 gaatcttttg gaaagtatct acgtattagc tatgacgcga agtcatcgga caagtagtga
      421 gcaggaattg attggctgga ctgacgatca ggcgatgatt caaggagaga tcacaaaagg
      481 ttcatccact cttccattag aaatcttatt atctaaaaaa ggccgaaaaa ccaagatcaa
      541 ccatatcgaa caaaaaaagt taagtactta cgtgggacaa atgaatgtca ttttatttgc
      601 accagaagat ctttctttag taaaaggcag cccgcagatc aggcggaagt ttttagatat
      661 ggaattaggc cagatcaatc caatctatct ttatgatctt gtccaatacc aagcaatttt
      721 gaaacaaaga aaccagtatt taaagcaatt gaatgaaaaa aaacaaactg atttgcttta
      781 tttggatatt ttgtccgaac aactagcagc gtttggcagc aaagtattga aagcaagagc
      841 acagttcgtc aagcgattgg agttttgggc taattctttg catcagcaaa tcacccatca
      901 aaaagagcag ctggaaatcg agtatctcac cgcagtcgat tctttagaaa cacatacgca
      961 agagcaaatc caagaacaat ttctcgcact tttgaaccaa aataaaaaga aagacctttt
     1021 tcggggaaca acgacagttg gtcctcatcg tgacgatctt tcctttttta ttaatcagaa
     1081 aaatgtgcag acttatggat cgcaaggaca gcaaagaaca actgcactaa gtgtcaaatt
     1141 agcagaaatc gatttgatta aagaagagac gggggaatat ccaatccttt tgttagatga
     1201 tgtgatgagc gagctggatg atagtcgtca actccatctg ctagaaacaa tcgaaggaaa
     1261 agttcaaacg ttcttaacaa caacgacact tgaccatgta aaaaataaaa tgactgttga
     1321 acctgagatt ttttatgttc aacaagggaa actaaaagga gaagaaactg aatgacagaa
     1381 gaaagaagtt tagtagaacg cgctaaagag tatgatgcca gtcagattca agtacttgaa
     1441 ggacttgaag ccgttcgtaa acgacctggc atgtatatcg gttcgactag ttctgagggt
     1501 ctgcatcacc ttgtttggga aattgtcgat aattcgatcg atgaagtatt agcaggtttt
     1561 gcaacgaaaa tccatgtgat tattgaaaaa gacaacagta tcaccgttat tgacgacggt
     1621 cgggggatac ctgtagatat ccaggcaaaa actggacgtc ctgcagttga aacggtcttt
     1681 actgttttac atgctggagg aaaattcggc ggtggtgggt acaaagtttc tggtggactt
     1741 cacggagtag gatcttctgt tgttaacgcg ctttctacac agcttgatgt caaagtatac
     1801 aaagaaggaa atgtgtacta tcaagaatat cgtcgtggag ctgtagtgga cgacttaaag
     1861 attatcgaac aaacggatcg ccatggaaca acggttcact ttacgccaga ccctgaaatt
     1921 tttacagaga caactgagtt tgatttttca aaattggcta cccggatccg tgaacttgct
     1981 ttcttgaacc gcgggatgcg tatatcgatt gaagacaaac gagaagaaga accagtcatc
     2041 aatgaatacc attatgacgg cgggattaaa agttatgtcg aatacttgaa tgcaaacaaa
     2101 accgtgattt ttccagaacc tgtatatttg gaaggtgaac agcaagatat cgcagttgaa
     2161 gtctctatgc aatatacaga cggctaccat tccaatatca tgagttttgc caacaatatc
     2221 catacgtatg aaggcggaac acatgaatca ggattcaaga ctgctttaac tcgtgtgata
     2281 aatgattatg cccgaaaaca aaagctgatg aaagaaaatg acgacaactt gaccggtgaa
     2341 gacgtgcgag aaggattgac agctgttatt tctatcaagc atcctgaccc tcaattcgaa
     2401 ggacaaacaa aaacaaaatt gggcaattcc gaagtacgga cagtcactga tcggttattt
     2461 agcgaacatt tcatgaaatt cttgctggaa aacccaagtg tgggccgtca aattgttgaa
     2521 aaaggattac tagcttccag agctcgtcta gcagcgaaac gtgcgcgtga agtaacgaga
     2581 cgtaaaggtg ctttagagat tagtaatctg ccaggaaaat tagcggactg ctctagcaac
     2641 gatcctgaaa aatgcgaatt gttcatcgtc gaaggagatt cggccggcgg atcagctaag
     2701 caaggtcgta atcgagagtt tcaagccatc ttgcctattc gtgggaaaat cttgaatgtt
     2761 gaaaaagcaa gcatggacaa gatcttggca aatgaagaga tccggtcttt atttactgct
     2821 atgggtacag ggtttggaga agattttgat gtatcgaaag ctcgctacca caaattagtt
     2881 atcatgactg atgccgatgt cgacggcgcg catatccgta cattgctgct gactcttttt
     2941 tatcgttaca tgcgtcctat tgttgaagct ggatacgtct atattgctca gccaccttta
     3001 tacggggtaa aacaagggaa gaatattacg tatgtacaac caggaaaaaa tgcagaagaa
     3061 gagttgaaac aagtcgttgc ttcacttcct gcctcaccaa aaccaagcgt tcagcgttat
     3121 aaaggacttg gagaaatgga tgatcatcaa ctatgggaaa caactatgga cccaagcaat
     3181 cggatgatgg cccgcgtcag tgtggacgat gctattgcag cagatcagat atttgaaatg
     3241 ttgatggggg atcgagtaga accccgtcgt gcattcatcg aggaaaatgc acactatgtg
     3301 aagaacttag atatttaatc atgaaggagg aaaaagaatg agtgaagaaa tcagagaaaa
     3361 catccatgat gtcaatctaa ccagcgaaat gaaagactcc ttcatcgatt atgcgatgag
     3421 tgttattgtc gcacgtgcac ttcctgatgt cagagatggt ctgaaacccg ttcatcgccg
     3481 tattttatac ggcatgaacg aattaggtgt aacaccggat aaagcccata aaaaatctgc
     3541 cagaatcgtc ggagatgtca tgggtaaata ccatcctcat ggagactatg cgatctatga
     3601 gtcaatggta cggatggcac agccattcag ctaccgttat atgctagtgg atggtcatgg
     3661 aaactttggt tctgttgacg gtgacggtgc cgccgctatg cgttataccg aagcacggat
     3721 gagcaaaatc gctacagaaa tgctgcgtga tatcaataag aacactgttg atttccaaag
     3781 caactatgat gatacagaaa aagaaccagt tgtactacct gctcgcttcc cgaatctttt
     3841 agtgaacgga acaacgggga tcgccgtagg tatggccacg aatatcccac ctcataactt
     3901 gtctgaggta gtagcggcta tcgatttgct gatggataat cctgatgtga cgaccaatga
     3961 attgatggaa gtattacctg gaccagattt tccaacaggt ggtttggtta tgggaaaatc
     4021 aggaatccga agagcgtatg aaacaggtaa gggttctatt accgtacgtg caaaagttga
     4081 gattactgag atgccaaatg gtaaagaacg gatcatcgtg acagaattgc cttatatggt
     4141 caataaagca aaattgatcg aacggatttc tgaactgcac agagacaagc gtatcgaagg
     4201 gatcacagat ttgcgggacg aatcttcccg tgaaggtatg cggatcgtca ttgacatccg
     4261 tcgtgacgcc agcgcatctg ttatcttgaa taacttatat aaattgacat ccttgcaaaa
     4321 ttcatttgga ttcaatatgt tggcgatcga aaaaggtgtg ccgaaagttt tgagcttgaa
     4381 acaaattctt gaaaactata tcgaacatca gcgcgaagtc atcacaagac ggacagtctt
     4441 tgaaaaagaa aaagcagaag caagggcaca cattttagaa ggtttgcgta ttgctttgga
     4501 tcatatcgat gaaatcattg cgattatccg cgggtctaaa tcagatgacg aagcaaaagc
     4561 gacgatgatc gaacggttcg atttatccga tcgccagtct caagctattt tagatatgcg
     4621 tttacgccga ttgaccggtt tggaacggga aaaaatcgaa aatgaatacc aagaactctt
     4681 aaaactgatt gaagatttaa ctgacattct ggcaagacca gaacgtgtta cagaaatcat
     4741 caagaatgaa ttagcagact tgaatcaacg atttggtgat aaacgacgca cagaattact
     4801 agtcggtgag gtcttaagtc tggaagatga agatttaatc gaagaagaag aaatcgttat
     4861 tacattgaca aacaatggtt acatcaaacg tttggcaaat aatgaattca gagctcaacg
     4921 aaggggtgga cgtggtgtcc aaggaatggg tatccacaat gacgacttcg tcaaaaatct
     4981 ggtttcttgt tctacccatg atacattgtt attctttacg aataacggaa aagtgtatcg
     5041 ggcaaaagga tacgaaattc cagaatacgg gcgcacagcc aaaggaatac caattatcaa
     5101 tttactaggt atcgattcga gtgaaagcat ccaggcaatc attgcggtga cgggagaagc
     5161 agaagaaggc cactatctct tctttacgac gcgtcaaggt acagtaaaac gtacttctgt
     5221 cactgctttt gctaacatcc gaagtaatgg tctgatcgca attggtttga aagaagacga
     5281 tgagctcgtc aatgtgctac tgacagatgg tcaatctaat atcattattg gaacacataa
     5341 cggctactct gtcactttca aagaagaagc agtccgcgat atgggacgta cagcttccgg
     5401 ggttagagga atacgcctgc gtgaggaaga ttatgtcgtt ggggcagcct taatgaagga
     5461 agatcaagaa gtattagtga tcacagaaaa aggttacgga aaacggacaa aagtatcgga
     5521 atatccagtg aaaggccgcg gaggtaaagg gatcaaaacg gcgaacatca ctgaaaaaaa
     5581 tggtccacta gctggattga ccactgtttc tggagatgaa gacatcatgc tgattacaga
     5641 taaaggtgtc attatccgtt tcaacgtatc gactgtttct caaactggac gttcaacatt
     5701 aggcgtacgt ttgatgaaga tggaagctga tacgaaagtt gtcacgatgg cagcagtcga
     5761 accagaagct gcagaacaaa cagttgaaga acaaggatca gaagaatagc catcaagact
     5821 aattgtgtaa gctagcaaga atgaccgatt aaaaaatctg gttattcctg ctggcttttt
     5881 tgattagttg aatttctctt aaaatattcg aaactgttca ttctgtattg cttttgacca
     5941 aaatagagtg tataatcaga gcttgtgagt agtctaatta attagataac tctccttgct
     6001 cttaggaacc taagagccga aagtccacag ggaggtgaat atcaatggaa aacacgaaat
     6061 atgaaatcat gtacattatt cgtccaaaca ttgatgaaga agcaaaaact gctttaatcg
     6121 aacgtttcga tgcaatcttg aaagataacg gagcagaagt tatcgaatca aaagattggg
     6181 aaaaacgccg tttcgcttat gaaatgaacg gttaccgtga aggtatctat cacatcgtta
     6241 aagtatcttc tccatcatca gcaaatgctg tcaatgaatt tgaccgtctt gctaaaatca
     6301 acgacgatat cattcgtcac atgattgtta aagaagaagc ttaattgttg tttcatgtga
     6361 aacaatagaa aacgatgaga ggagatgact attttgatta ataatgttgt attagttgga
     6421 cgtctgacga aagatccaga tttgcgttac acagcaagtg gtacggcagt cgcaacattt
     6481 actttggctg tgaatcgtaa ttttacaaac caaagcggca atcgcgaggc tgattttatc
     6541 aactgtgtga tttggcgcaa atcagcagaa actttagcaa actatgctcg taaaggaaca
     6601 cttttaggtg ttactggacg tatccaaact cgttcttatg ataatcagca aggacaacgt
     6661 gtatacgtga cagaagtagt agctgacaac ttccaattgc tggaaagtcg ttctgcatct
     6721 gaaaatcgcc aacaaagcgg cggattccaa agttctggtc aatcagctgg cggatttggc
     6781 ggaaacaata actctaatca aacatctcaa tcatccaacg gtatgcctga tttcgaccgt
     6841 gatacttctg acccattcgg ctcaagttca acgatcgaca tttcagacga tgatttacca
     6901 ttctaaacga atttgatagg agggaaatag aatggcacaa caaagaagag gcggacgtaa
     6961 acgtcgtaaa gtagactaca ttgctgctaa ccatattgaa tatatcgatt ataaagatat
     7021 cgaattacta aaaagattta tttcagaacg cggtaaaatc ttgccacgtc gtgtaactgg
     7081 tacaggcgct aaaaaccaac gtaaaattac tgttgcaatc aaacgcgctc gtatcatggg
     7141 cttgctacct ttcgttagcg aagactaata aatcctagga attgaatgac tgatcctttt
     7201 gatcagctat tcaattcctt tttttctgta aaaataacct ctattcgttt agtcgctcga
     7261 atgacgaaag ttatggtaaa atcaaattag tcatatatgg gaggaaaatt atggaaaata
     7321 aaaacaatca tttatccccg aaacctctct tccttatcct gtttatatta atcgaagtat
     7381 tggtagtctt aattttacca tttcgttttg ttagcgcttc ggtcattcta ttgatgaatt
     7441 tatggggtgt tcgctattgg ttgaaaatga aacgttggct tattttaaat aaacgtgaaa
     7501 aaatccagca ggcttcgact gtagctgaaa ccaatctagc ttatgtctca gaagtcatgc
     7561 ctgtaggagt gattgcttat cagccatcaa gccaaaaaat cgaatggctc aatccattag
     7621 cattgaaaat caaagatcaa agtggattaa cagaggaaga attgctagat acttttttta
     7681 ctgcaaaaga aaaaaagaaa aaccaagtct atctagcaga taaaacattt cagtttgata
     7741 tggatacagt aaagggtgtt gtttatttta atgatattac ccaaaaccag ttgttaaaac
     7801 tccatcagac gaacagtcag ccggtgattg gtattttatc tattgataac tatgcagatg
     7861 ccattgataa attggatgac aaagagattt cttatttgaa ctctttcgtc actacactag
     7921 tttctgactg gatgaatgaa taccatgtct ttttcaaaag aatcaacgca gaacggtttt
     7981 tcttcgtagc acagatgatc gatctgaaaa agatgatgga taaaaaattt gatttgctgg
     8041 atcgctttcg agcagaagct gaaaaacaga gctcgcctat cacccttagt atggggattg
     8101 cttatggctc aagaaccctc gatgaaatcg gtggagaagc tcagaataat ttagatgttg
     8161 ctttagtccg tggaggcgac caagttgtcg tgaaagaggt aaaagaagga gcaaaacctg
     8221 tttattatgg ggggaaatct gctaagaatg tcaaacgcac gcgtgttcgt tctcgagcga
     8281 tgagtaccgc tttgcgtcgg atttttgatg aaacaggcga tgtgtttatc atggggcaca
     8341 agtttccgga tatggatgct ttaggctctg cttttggtgt cagttgtctg gcacgcttca
     8401 accagaaaaa agcttacatc gttatcaacg agaacgagat cattccagat gtagaacggt
     8461 gtcttgaaga aatccataag catcctgaat tagaaatgca actaatctct ccagaacgtg
     8521 ccatagagct gaaaaaagaa gaggacctcc tcgtgatggt ggattaccat aaaccttctc
     8581 tatcgatctc tcagggccta tatgagcaat ttgataagat tgtgatcatt gatcaccatc
     8641 gtcgtgggga agagtttcca agtaaacctc tgctgactta tatcgaatct tcagcttctt
     8701 cagcttcaga gctagtttct gagctgatcc aataccaaag cagcaaaaca aatcgcttag
     8761 atcgttttga ggccacatta ttgcttgcag ggatggttgt agatacaaat agtttcaatg
     8821 tccgtacaac tgcacgaact tttgacgttg ccagctattt aaggtcttgt ggagcagacg
     8881 catccttagt acaatattta ttgagctcgg atttagcgac atacttggag atcagcaatt
     8941 tgattgctgg aagcgactat gtgacttctg atattgttgt ggcggcaggg acagacgaaa
     9001 aagagtataa tagtgttata gcagcaaaaa cagcagatac attgctttcg atgatcaata
     9061 tcaatgcagc tttcgtcatt acaaagcgga cagacggctt gattgggatc agtgccagaa
     9121 gtaccggcac gatcaacgtc caaatcatca tggaatcctt aggtggcgga ggacacttca
     9181 caaacgcagc tactcaactg gaaaatacga cagttgagga aacaaaacag aagctatttg
     9241 ctgcaatcaa agaaaatatg aatcaaatat atgataagga gtaatccaaa tgaaagtaat
     9301 ttttttacaa gatgtaaaag gaaaaggtaa aaaaggggac atcaaagaag ttccagcagg
     9361 ttatgcacaa aattatctaa tcaaaaataa cttggcaaaa gaagcaacga aaagcagtat
     9421 cagtcagtta gcaggacaaa agaaagcaca agaaaaacac gaagctgaaa tcttggaaga
     9481 agctaaaaag ttaaaagaat tcttggaaaa agaagatact gtagtcgaat tgaaggcgaa
     9541 agcaggggaa gacagccgtt tgtttggttc gatcccttca aaacaaattg ctgaagggtt
     9601 aaacaaacaa tacaaaatca aattagataa acgaaaaatc gaattagaaa atccgatccg
     9661 ttcactaggc tacacaaacg tacctgttaa gcttcatcat gaggtgacgg ctacgatcaa
     9721 agttcatgtc gtacaagaat aacaaatctt gatagcgatg ttcagctggt aagtataacc
     9781 agaaggaaaa aagacaaacc gaaatttatc tgagaaaaca gctgcagcaa tgaaagcaaa
     9841 agctgcaaat cgatttcaga ttgatttcgg tttctttttt gggaacagca aaatagcata
     9901 aagaaaacga gtataataaa agagaactat ttttttatta cactttcggt acgaatgatg
     9961 acttttctct cattttcttg taaaatgaag caattgaaga caaggagcga tacgtatgaa
    10021 tgaagtgtgg caggatcgaa tcccaccgca aaatattgaa gctgaacaag cggttttagg
    10081 ctctgttttt cttgacgcag aaacgatcat tgatgccatg gagtacatag agccaaaaga
    10141 cttttatcgt cgtggccatc aaattatttt tgagacaatg atcgagttaa atgatcgtaa
    10201 tgaagcgatt gacgtggtta ctgtcaaaga ccggctggaa caagccaatt tgttagaaga
    10261 tgccggtggt ttaagttatc tctcagactt ggcattagca gttccgacag cagcaaatat
    10321 cgtctactat gcgaaaatcg ttgaagaaaa atcattgctg cgtacattga tccagacagc
    10381 aacaggaatc gttacgaaag gattcgagca aggagaagaa gttcaaacaa ttttagatga
    10441 tgccgaacgc agtatcttgg aggtttcgga aaaaagaaat cgaagcggct ttttatctat
    10501 tgctgaagta ctgaactcca cgattgaaaa tatcgatcga ctagctcaaa acaatgaaga
    10561 aatcacaggt ctaccaactg gctatcaagg tctggataaa atgacagcag gtttccagcc
    10621 cgaagaacta atcattcttg ctgcacgtcc agcggtaggt aaaacagcgt ttgcattgaa
    10681 tattgctcaa aatgtaggaa caaaaacaga tcgttctgtt gcgatattta gtttggaaat
    10741 gggggcagaa tctcttgtaa atcggatgct ttgtgcagaa ggttcgattg aggccagcca
    10801 tttacgtaca ggtcagcttt cggaagaaga atggtcgaat ctgatcgttg cgatggggag
    10861 tctttctcga gcaaatattt ttatcgatga tacaccaggg atcaaaatct ctgaaatccg
    10921 tgcgaaatgt cggaaactag cgcaagaaaa aggcaatcta ggcttgatat tgatcgatta
    10981 tttgcagttg attgaaggta acggtaaaga gaaccgtcaa caagaagtat cagatatctc
    11041 tcgccaactg aaaaaactgg cgaaagagct gaaagtacct gtcattgctc tttcacagtt
    11101 atctcgtgga gtagaacagc gacaagacaa acgaccagtg ctaagtgata ttcgtgaatc
    11161 cggatcaatt gaacaagatg cggacatcgt agccttcttg tatcgagatg attattatga
    11221 aagagaaggc ggagatgacg atgatggaaa cgagccaaaa aatgacaacg tcatcgaagt
    11281 catcatcgag aagaaccgaa gcggtgcacg aggaacagtt gaactactgt ttatcaaaga
    11341 atacaataag ttttcgtcgc tttcgccaag agaagaattt taaagaagag gttgtgagcc
    11401 ttgagcaatc ccgagagctc tcgtaagaag atgcaaggag taaccgcagg aacgactaga
    11461 gtacttaatt taaacagatc tccaaaggga ttgtgacaaa agtcttgtca cagtcccttt
    11521 ttccgaataa acggtgttca atcagctgac ccttggtatt agctcagcaa acgtcaaaat
    11581 atgaggaact attttgacgt ttgtttctct aatactcagg tcaaaacgct gatcttacga
    11641 cctcttttcc ttaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatcatc
    11701 aaacatgaga agcagttttt tgaaattgct ccttgtgact cagagttaaa cggcaggctc
    11761 acaacctctt tcttaaacgg tgttcaaaag cttgcatctg cggtaatacg cccaatcaaa
    11821 cgaaaaatat gagaagctat ttttgtttgg ctgttcttat tacttgatgc aggacagctt
    11881 tttcacaacc tctgattcac ggtgttccaa aagtagcttc ttcgaaaaat aagtcgaaat
//
LOCUS       DAYYHX010000076        11901 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000076 DAYYHX010000000
VERSION     DAYYHX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.077"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(178..1197)
                     /locus_tag="ACHA6Y_001539"
     CDS             complement(178..1197)
                     /locus_tag="ACHA6Y_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8987636.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            complement(1208..1405)
                     /locus_tag="ACHA6Y_001540"
     CDS             complement(1208..1405)
                     /locus_tag="ACHA6Y_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8987637.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            complement(1428..1721)
                     /locus_tag="ACHA6Y_001541"
     CDS             complement(1428..1721)
                     /locus_tag="ACHA6Y_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987638.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERSR"
     gene            complement(1758..1895)
                     /locus_tag="ACHA6Y_001542"
     CDS             complement(1758..1895)
                     /locus_tag="ACHA6Y_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8987639.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEAEKIINKDKSA
                     S"
     gene            complement(1897..2343)
                     /locus_tag="ACHA6Y_001543"
     CDS             complement(1897..2343)
                     /locus_tag="ACHA6Y_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987640.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRADGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADGLAQINSKTMLASFQNSKDIAEIKEQLNPASKGGE"
     gene            complement(2346..4532)
                     /locus_tag="ACHA6Y_001544"
     CDS             complement(2346..4532)
                     /locus_tag="ACHA6Y_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8987641.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNAALTNVDSTIEKANQQINEFVEGATQAIDQTVDEVTEQLQATQTKIDTVSQN
                     VTSAQNNLKAVEDKMNQTNQQIGDLGKLKKMYSNSIDFGNYDYSGNPNLMSKLKSSDF
                     NVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAI
                     DKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFV
                     DRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWLGKAPLGENIANKSVTFPIKSSA
                     YSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRYGNLEPVEGLVDTWG
                     LTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGDTRTPNISQFKYFGEGLKD
                     SNNPNDYSWDVTPEYTEKGLNDAVNVYDPQRVEGLKNFADGIQIAGDKVISENDCTVY
                     TLTKDNSQSFIDGYATFIKHGKEVIVNGTVKFKKAYAFGVPLDDEVPDEFVAKIVHGM
                     LTGPSGTSSVSKAMYVRKDLGTIITNSEFAANEWFTFHGNYWVGEE"
     gene            complement(4532..5314)
                     /locus_tag="ACHA6Y_001545"
     CDS             complement(4532..5314)
                     /locus_tag="ACHA6Y_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987642.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPDGIEDDSF
                     VEMMVFADHDSRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTTSVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNGDAGVTFIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNNVKITKIEGVK"
     gene            complement(5331..7088)
                     /locus_tag="ACHA6Y_001546"
     CDS             complement(5331..7088)
                     /locus_tag="ACHA6Y_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8987643.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYVDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            complement(7063..9894)
                     /locus_tag="ACHA6Y_001547"
     CDS             complement(7063..9894)
                     /locus_tag="ACHA6Y_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8987644.1"
                     /translation="MSETVYFFDHLQKLIKRKNTRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPFETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPTVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPESTQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTDLLKNTYSTLIDSARPLVQFKAEVTGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPEVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            complement(9891..10679)
                     /locus_tag="ACHA6Y_001548"
     CDS             complement(9891..10679)
                     /locus_tag="ACHA6Y_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="distal tail protein Dit"
                     /protein_id="HFM8987645.1"
                     /translation="MDQRENKMYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGY
                     QTLTVEGREMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELL
                     NYLYRNEDVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVS
                     DGYIGNPIQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQV
                     TVNGEDCTSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            complement(10691..>11901)
                     /locus_tag="ACHA6Y_001549"
     CDS             complement(10691..>11901)
                     /locus_tag="ACHA6Y_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8987646.1"
                     /translation="VNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSV
                     VNIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMIWIDLKLFFTNL
                     WIDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLIKSWNS
                     LKQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNIIQGWENLKQGTIDTFNNLVN
                     GAQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKSVQDFV
                     GGIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFNDVQNTVGSMADFIAELF
                     NANSDVDIAANLKNANKNIGAQVEHKINMGGSTKPAVFKINLGRQSFRLFMDDISQAM
                     GEGADINLEF"
BASE COUNT         3201 a         2600 c         2010 g         4090 t
ORIGIN      
        1 gcttctccaa taaacctaac atttgcttgg ttacatgctg cttctatttc ctctttttta
       61 actataatta agcacctctt ctcactaact attaaattac cgcattttta caagataatc
      121 aataagttat ggacaaattt gcattatgta cttcccctca aaaacgaggg gctattttta
      181 tcgttgcgga atatttaaat accagcgttt atcatgaaaa tcttgtgctc cgcctttagt
      241 gttcccttct ggatcattcg ttgcccgcat cattacatag actttcttat tagggaagtt
      301 gcgcatgtta aaagatacat gatagccaac atttcctaaa gtattataag cttgatttac
      361 atctggtcta tatattccat cagcttttac tcgagctaat tctttcccag tattgtaatc
      421 cataatgaag atatactcgt atttatagtt agcgatgtgc caaccagcca catgtaagtt
      481 tgcgttttcg atttctccga actgatcaat gtgggcgtaa tttgttccat ctgtcagggt
      541 aggatttgca gcacctgctc gagttggatc aatgactggt ttatcatctg aagttgtcgg
      601 attttcatcg gtaaatccat gagccaaatc ataggctaat ttttctttac ttacgcccat
      661 ttcagaaaga taaccgtaag gatctgtatg atcgccccaa atattttgtg ttacccataa
      721 atgcgatttg atccctggtt gattatacgg tgtgtctagt gtaagtggaa taccatattt
      781 cattgctgaa tctctagcta attcaacgta tgccttgtag tttttctcaa acgttgcttt
      841 atcatgtgtg tgttgtaact caatctgcac aggactattt gcattagcat acgaaccagc
      901 accgtactgt acataaccag gttgaccgac ttgataaaca attccgccgt ctcccacaat
      961 ataagcagta taagcgctag tccatgaacg ttgcatatac tgcgcttcat tgcgtcctgt
     1021 tgctgtttca ttagccgttt catgcagtaa aatgtactga ttatttgcta cttgtgagct
     1081 accttcgttt gggcctaaat taaattcatt gttaatcgta taggcaaacc cattaattgg
     1141 caataaaaaa agagccgtta ataggctcat cgcagtaata gtaattttct ttttcatttg
     1201 tttcctccta ttttttcaaa ttataagccg acacgccagt gataacgcct aaaaatgttg
     1261 ctactgcatt gatagtgagt actgtcatat ctgttccatt ccatccatac gctttgccta
     1321 gtgttgcaac taatacagat gcagctggca atactgttaa aactgtccat ttaatgactt
     1381 gataatactt atcgggtaaa atcatttctt cttaactcct ttctttttta ccgagatctt
     1441 tctctaaata aagttttgat ttgttgtgtg tgttctacca atttttctgt atgtgtatct
     1501 aatctttcat cgtgtttctt tagttcttca tgaatcatca atcgatctga tttgctcgat
     1561 tctaaatctt tagtcagcaa atctaaattg tgacttactt ttgaaagagt ttcagtaatc
     1621 ttcgagaaag atgcagtgac tggtcttatt actaataaaa tcaaagaaac aatcgcagtg
     1681 attgatcctg cgattgttcc ccattctcct aaattaatca tgtgacaact cctttacctt
     1741 aaataaaaac gcatcactta agatgcgctc ttatctttat taatgatttt ttctgcttct
     1801 tcgtctgtaa tgcatagtgg aacgaatagt cgaacttgat cgtcagtaaa acagccccaa
     1861 tcatacatca ttttcacatc gctaaaacta aacatactac tcacctccct ttgaagctgg
     1921 atttagttgc tctttaattt ctgcaatgtc tttgctattt tggaacgaag caagcatcgt
     1981 ttttgaattg atttgtgcta aaccatccgc tttttctttc aatgcagtat tttcctgttt
     2041 aatcgctaca tcgcttagca tgagtttggc gttgagctgt tttaaatcgc cgttctcatt
     2101 ttctaacgac tcatacatcg ctttgagatt gtttaaatcg ttgtgatcta gtacattcac
     2161 taaaacaatc cattggttca gtttaggatc aaacatctga tcagcaatcg ttagtggttc
     2221 cccatcagca cgaattcctt cgagtggtgg aacatcggta aatggaacag tcataaccat
     2281 atcatcaagc acttgtccag catactctcc acctgttcgt ccgtatttcc aaatattttt
     2341 catttttatt cctcccctac ccaataatta ccatggaatg taaaccattc atttgcagca
     2401 aattcgctat tcgtaataat tgttcctaag tcttttcgca cgtacatcgc ttttgataca
     2461 ctacttgttc ctgacggacc tgtaagcatg ccatgaacaa tttttgcgac aaattcatct
     2521 gggacttcat catcaagtgg tacaccaaaa gcataagctt ttttgaactt tactgtacca
     2581 tttacaataa cttcttttcc gtgtttaata aatgttgcat acccatcgat aaacgattga
     2641 ctgttgtctt tagttaatgt ataaacagtg caatcatttt cactgatcac tttatctcct
     2701 gctatttgaa taccatcggc aaagtttttc aaaccttcaa ctctctgagg atcatacaca
     2761 ttaacagcat cattcaagcc tttttcagta tattcaggtg tgacatccca actgtaatca
     2821 ttgggattgt tactgtcttt caagccttca ccaaagtatt taaactgact aatattcggg
     2881 gttcgtgtgt cgcctttttc gatcttgagc cagtcaattt gacatgcacc tgttgttgat
     2941 tggggatact ggataatagt taaccgtttg gggtagttaa ctccaacctt ctgcggtgta
     3001 aaagtcagac cccatgtatc aactaaccct tctactggct ctaagttacc atatctataa
     3061 tctccactat cctcgttgta aacaatgaac gtttggatag gtggtttggt tgccttcatg
     3121 gtaattgtat aggtttgacc taaaacaaat tcttcttcca tgttagcttg gtataggctg
     3181 taggcgctag atttgattgg aaacgtaaca gacttattag caatattctc acccaaaggc
     3241 gctttaccta gccagtaagg gtcatcaagt aagtttggct gatatggggt ggctgttgag
     3301 ccttcttcga ttttgatgtc gtacaactta aatccgccat ttattttatc cctgtcaaca
     3361 aagctaatac tcatataaaa tcgatctaaa tttgtgattt gataattaac gttagctgta
     3421 cctttgattg ttatttcttt ccctacatca tctgttgtaa tatttgtact atttgcttcc
     3481 aataatatct tttctcctgg tgatgtacga tacaccaaac gtaatttatc aatagctcct
     3541 gtagttcctt catcaaatcg aactttcgca ctcagagtat aggttttccc actagcaagc
     3601 tgaggtgtat taatacgcgt aaacataata atgcttcctg taccatcaga agtaaaatgt
     3661 agcttttcgt tatccgaagt tagtgacccg tggtaaccaa cgttaaaatc gctcgatttt
     3721 agtttggaca ttaaattcgg attcccacta taatcataat tcccgaagtc gatgctgtta
     3781 ctgtacatct ttttcagctt gccgaggtcg ccgatttgct gattggtttg attcatcttg
     3841 tcttcaactg ctttaagatt gttttgtgcc gatgtaacgt tttgagaaac ggtatcaatc
     3901 ttagtttgag tagcttgtag ttgctctgtt acctcgtcaa cagtttgatc aattgcttgt
     3961 gtggctccct ctacaaattc atttatttgt tggtttgcct tttcaatcgt agaatccaca
     4021 tttgttaatg ctgcgttaac agcatctgtt gcttcttgtt tgactccatc aagtaattgt
     4081 tgaaagtctg caataaagta gtctgcttta tcttgcgcta gtccgtcgat tgctgaacgc
     4141 ttcatacgga aggtgaagcc taagttatca cttgttgatc cgtctggata ctcgatgtaa
     4201 acataagctt ccaccatacc gttataagct ttcatcgctt ctggcaagat atatacgacc
     4261 tgtccattca agaagctttc agtgatgagg tttttcgtga taaaagggat tggctctttc
     4321 gtgatcattc catctacttc atcatagata aacatcaaca agcgcaagtt agctcctagc
     4381 aagtccgccg gtgtgccgtt ctgttgttcg acgttgaact ctaacgctat ttgattatcg
     4441 tatgatttaa atacaagccc cgttgcttgc aaatcgtagt ctttcggctg tgtaggtact
     4501 ttgatagagc cttttttgat gacatgcgcc attatttcac accttcaatc ttagttattt
     4561 tcacgttgtt gctcattgcg ggattggaaa cattgccgga tgaaatatct aaagctctgc
     4621 cgtttgacat cgttatcttt cgcgcttcga ttgtcagctc aaattcgatg aacgtcactc
     4681 ccgcgtctcc attccataaa ttcgcttttg taattcgcgc gtatctttga cgtttcactt
     4741 cttctacaaa gtctccgtca ccgtctgtgt agtggatacg aagcgtttgg taacggaaag
     4801 tatcatctgg cagattgact gattgtcctt ttcttaattc gccttcaaaa agaacagaag
     4861 tagtgtaaac gcgccgccag cctaacgagt tataaccctc taccggttcg ctgttatgtg
     4921 tcattttgat gtaaatttcg ccgctataac gagcaaaagc gacgattaat tttctcgaat
     4981 cgtgatccgc gaataccatc atttcaacaa aactgtcatc ttcaatcccg tcagggttgt
     5041 ctgcgcccca tccggctttg gttgcgtatt tgccgggcgg aatgtctaaa atgttcggat
     5101 atttttgtgg tagcttataa tcgcgtaacc aacgcccacg cgcttgcata actgtttccg
     5161 gtgtagcaaa gcccgcgttg ctttcagtag ctaggacgtg cgcgtcgtga tccgagccgt
     5221 cgtaatggat agccgcttga cgcaataacc atttcacagc ctcttcataa ctcatttctt
     5281 caaaaatatt tggtttgctt tgaaaatcta acatttaatt gttcaccgcc ttaatagtta
     5341 taagtgaagt ctctcgcgcg ccctacgcct ttgcttgtca cttttcttgt tgctgtgtca
     5401 acttctattt tataaaacgc taattcatcc ggtgtatcaa cttggctagc tgtatgagag
     5461 aagccgacgt cgcaaagaat catttttact gttccaaacg ccccttcgac ttgttcgtgc
     5521 caatgcccgc agaaataacc tactacaacg ccggcgcctt ttgtattcat tgcgaaggtc
     5581 tttaatccgc cgaacgaacc gtcattcggt tgtcctaatt tagaccaatc gatagttacc
     5641 ggactaccag atttaaaccc ttctatcaac gtgctaatca tgttttcatt gcgaacggga
     5701 aacttgctga gacctaaagg cgtatgtcct actaacgcca catgatagtt tctcggaacg
     5761 ttcactaacc attctccgaa agcgttaatt tgtttggcac tgaaagctcc cggcgctgta
     5821 tcgctgtaac cgtccacgta cttgtcgcct gtgccgcctt cataaaaatc acaagtatcg
     5881 aaacggtaaa ttgctgcgtt tttatctggg aacaacacgc caccgtacaa accgttccaa
     5941 tactcttcga agtcagcgtt gcataacatt ccctttcgct tgcgccacgc ggggtcgaaa
     6001 caagcgtcgt gattcccttt gcagataata accggttttt cttgtcctgc taccgctgcg
     6061 ttagtgaagc gtttgagcgt gcctaacatg gaatgtcgcg cgctccattc atcaataatg
     6121 cctatatcgc tgccgagtga accaagtccg ccgtcgatat tgtctccgcc gtaaatcatc
     6181 acatctgttt tgttccctaa tttttgaaat tgagggattg cgcgccaatg tcttaaataa
     6241 gaagcatctt tgtaaccgac gccatctata cgcaagttgt gattatctgc gtgaatgtct
     6301 gtaatgaaag aaaagttaaa tttgctgtta ttaacactgt tgacaaccgt attcaaattg
     6361 cgcggcacta ggtcaacgta tttcatcgtg tcgtaatcaa aaaagcgtgt tgtttctcta
     6421 atttgtgagc tgccgactgg cacttggtat ttttggttta aacgatcagc taatgattcg
     6481 tagtcgcctt tagcttcatt cagaatgtta atgatcatac cacctggatc gatattttcc
     6541 agtatttcac gattatcttc taaccactgc tcccagtcat ttttgccctg atccatgtaa
     6601 tctttgaatt ttcttagcaa atcctcaaat gtccacacat agccagagtc acgtaactgg
     6661 cttctagata ttccagaaat gactcgatag gtaaaatcct gtgtgctaaa ttgttcactc
     6721 caagttccat caccattaag tgatcggaaa ctgaaatgtg cggtgttttc accaccccat
     6781 tgccagtcag gctcacttaa ggtgtaaaca agccttgctt gcgctggact gtattcttgt
     6841 actttttgtt caacaggttg gttttcgcca aattttgttg tattaataaa aaacggcact
     6901 aggccctcga atgtttttag tttgccatgt tccaccactt caacaacgaa cttttgcgtt
     6961 aaaacatccc cttgccgaat tcgaaccaaa tttattccgt tgtttggttc ggtggtggat
     7021 aggaccattt tatgctgcgt ttctgccatg actatccctc ctttagaaat cgatgtagtc
     7081 tcttgcattg tggaaatgac ctgaagaaga tggataaaac tcatccataa attgaaaatg
     7141 aagatgttct ccagtggatg gtcctgttgt ccccatcagt ccaatttgct gaccagcagt
     7201 taccttctga ccttttgaga cgtctactcg gctttgatgc gcgtatcctg tatacattcc
     7261 atcagcgtgt ttaatcactg tccaatttcc ataccagtca aagtaatttg catcacctgc
     7321 aacaatcact tcgccatccg ctgaagcaaa aataggtgta ttaggatttc catttacaag
     7381 gtcaataccg ttatgaaatt cttgtgctcc tgtgattgga gaagtgcgcc agccaaattc
     7441 gctcgtcact gtgattggat ccgcaatcgg cttcatatat ccttttgata caggaatttc
     7501 caaatctttg aacttgtcat accattcttg tgaccatgcc gtccgttctg gatgtggatc
     7561 acgtggacgt tcaaagttag ccacgaatgc ttgtgctgct gtgttgatat cggtcagatt
     7621 catgaattgt gtccatgtgt aaggataagc gctagttgcg atccattgac catttggtgc
     7681 atgccacatc aacaatttaa actgtgccgt gattgtgtct ggatcatcac tgatgccagc
     7741 ctttgtcatt aaattgatca tgtagacacg tccgctagtt gcgccagaag aatcggtcca
     7801 ttgccataca ccataaccga accctggtgc gccattgccc tcatcagcgg ttggattagc
     7861 atctgattct ccttgtgcat ttccaagtaa ggctgcagca gcttgttgtg taaaaccggc
     7921 acctattgcc gttgcccaaa tttgccaata tcgtttatcc cgatcagtag ttacttctgg
     7981 tgggtattgt ccgttccaac cgttatcgtt ccctccggaa ttgcctcctc cattagtgtc
     8041 aatttttgta ccgttaacat agagttcttt tacatctagc cgtccatcta tggttatatt
     8101 tccttctgaa aaattgccgt caccataaag attatatttg cgcttatcag cagtaacatc
     8161 tgctggaatt tgaaaaatag gattccctcg atcgccaccg tccccagcat taatggaaaa
     8221 aatatagttt ggctctttcc agacagcaaa gccgttaatc cctttagaac cataagtaga
     8281 tacaattgag cccaagcgtt ctccatgtac atcatctagt ccagtagact tgacttgttt
     8341 ttcaaaagaa agttctcccc cctctgccac taattggaaa tctttatcgt caaatgtttt
     8401 taaagctacc ccttgcacga ggataccaga cagaatgcct gcgcgaataa aattagcatt
     8461 gaaagttcca tccaacgtcc aagcggtcgt gctatcacca ttgtgtacat cttgaattgt
     8521 ttgccactca ccttttttac attgtttgaa agatatccct gagttatttt ggaccataaa
     8581 aaagcgtgat ctaggaatgt taggtccatc catataaaca gtttcataga tttctctact
     8641 atcactaaca ccagcttcaa ttccatttac ccaataaata gaaccgccat tatctcctgc
     8701 acctcgcata atgtcatctt gatattttcc aatctctgtc gattcgtaaa atgtcatttt
     8761 gctagattct aaactattaa tattattgac aatagaagcc gtttgttttc taacatcttg
     8821 tgttaaatta tcccctagtt cgatattcgt ttgaccggta agccgattga atgtagtttt
     8881 ataaatacga gttttatagt gataaccttt atcgtatctg tgaatagtca ctgtatttcc
     8941 tatcacatct cctccagtga cttcagcttt gaattgtact aacggtctag cagaatcgat
     9001 taaggttgaa taagtatttt taagtaaatc tgttggatca tctatatcat caaacactac
     9061 tacggtttct cgttttctca ttgatccatc tttttgtggt atcccatact tttgagttga
     9121 ttccggatct tcaagccaat tttggccttt aggcttatct aaaggatcac cattcgactt
     9181 tttccattca acatcagtga attcaattct tctaccgtaa ccgtcaccaa cctcttcgcc
     9241 tcgcccacga cctatcattg aagttgagat tgagcttcta tctatctctc ttacaactgt
     9301 taatgcttta ctaccatata cgaaacgtgt attcgattct tcgccaattt gttcatatac
     9361 ttcgatccat ttatccttta ttccatcaga attcaaagaa cacctaaaga caaattccat
     9421 acctaaggtt tgcaattctt tcaacgcttc ttttacagag acatagtaaa aagttgcagt
     9481 tactgttggt aacattgctt ctacgtgacc aacgcgccaa tttccttcag taaattcaat
     9541 caatcgatca agaacatttt ttaagggctg cccactcggc ctaatatctt tgatgatgta
     9601 agcatctaat tcatttgtcg caaatcctaa ccctgtaaac tctaatgttt caaatgggtc
     9661 gctaacttta gtaattcgat acaacgaaaa agacgactcg ttttcacgaa tcgccatata
     9721 tcttgcatcc tctatttctt tatcatattt tgtcgtaacg taaagagtat ctttcattag
     9781 atcactctta tcagaactaa tttctttttc ttgggagact tcaatcaaac ttcttgtatt
     9841 ttttctttta ataagttttt gcaagtgatc aaagaaataa actgtctcac tcaaattgtc
     9901 gcccccctat agaatatttt aagcttccca ttattgctag ttatcttctg accttgctta
     9961 agataaaagt tctcaaaatc actttctaaa tcaatcatag aagtacaatc ttctccgttt
    10021 acagttacct gctcatcgga aaaatcaaaa accaacacgt ctcctgtttt tattgccgcg
    10081 tcagttatcg tgatattttg ttctccgttt gtaattttga ttgaattatt catggataaa
    10141 gtaacttcaa tttttcttgg tgttatagga aactgtatcg gatttccaat atagccatca
    10201 ctaacacact ctttcgtata ctttagtgga tccgcacaga atacattaaa actcgaaata
    10261 atcgagttgg agtctcctgg aacagtatca gttgatgtat agcgaccgta gtaataataa
    10321 tctaattcat catgaaacct aatttccacg tcttcattcc ggtataaata attcaacagt
    10381 tctttgaatt taaactgtag tttttctgga tctctatctt ccagcttata tgttattttt
    10441 agtgttcttg aaggtatttt ctgatttgta atgattgaac caatttgtat ctcttgctgt
    10501 tcaacttcta cagaaagcat ttctctacct tccaccgtaa gtgtttgata accatcaatc
    10561 aaatcttcta aatacattcc atcgtacatc atggcagacg tcggaaggaa tcgtttagaa
    10621 ctagtgagat taatggttgt atctttgaat gagtacattt tattttctcg ttgatccaaa
    10681 atattccctc ctaaaattcc agattaatat ctgcaccttc gcccatagct tgtgaaatat
    10741 cgtccataaa caatctaaac gattgtcttc caagattgat tttaaataca gctggtttag
    10801 tagagccacc catatttatt ttatgttcaa cttgtgcacc aatgttttta tttgcatttt
    10861 tcaaatttgc agctatatct acatcagaat ttgcattgaa aagttccgcg ataaagtctg
    10921 ccatacttcc aacagtattc tgtacgtcat tgaatcctcc tgtcagacct ttattcagac
    10981 cgttcataat agcctgacca gctggaatca atagctttct atcgtattgg ataggtcctt
    11041 tgtgttcacg aatccaatca ccaatacctc caacaaaatc ttgcacagat ttccatgcat
    11101 tttgtaaccc ttctagaaaa ctatccatga tagcttttcc ggctgctagt aaatcgatat
    11161 ttttcaagtt atcaaaccag ccagttactc tatcaaccgt gtcactaaca gcatttacta
    11221 aattatccca tgcctcttga gcaccattta ctaaattgtt gaaagtatct atagtacctt
    11281 gttttaggtt ttcccatccc tgaatgatgt tatctttggt tccagtaatt aaatcaccaa
    11341 tccaagattt gaatgaagtc caaatgtctt tagcaccttg aactgtattg ttgaataaat
    11401 ctatcgttcc ttgctttaag ctgttccaac tttttataag agtttgcacg atattattaa
    11461 ccgtttggaa aaaccattgt tttagattat tccaaagttc aattgcacca tattttgtat
    11521 caatccaagt ttgaattatg gagaatttta gatttatcca catttgaatt gcactgtatt
    11581 taatatcaat ccataaatta gtaaaaaata gtttcaaatc aatccaaatc atagtcgctt
    11641 gataaactac ttcattccaa atattagaaa tactttgtat gaatccagtc cataaactaa
    11701 cggcactatt tactattgca ccgatatatt ccgtaaatat attttttaca gatgtccaaa
    11761 tatttaccac tgattggaca agtgtgttcc agattagatc taaatcctct ttcatttgtt
    11821 cgaagtcacc agtaattaaa tctataataa acagtagtgg cgcagctatt aaagacttaa
    11881 tgatttccca agcatttact a
//
LOCUS       DAYYHX010000077        11818 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000077 DAYYHX010000000
VERSION     DAYYHX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.078"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..730
                     /locus_tag="ACHA6Y_001550"
     CDS             <1..730
                     /locus_tag="ACHA6Y_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8987647.1"
                     /translation="PLCISYIPVGLACGVLLQKVGFDPLLSGLLSILVFSGGAQFLVA
                     SMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTFLAVFSQSLNDENYAVNY
                     LKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRVDADLVHFALTAMFIFMF
                     IMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIASFAGFMLEKAFKKEKAKK
                     LRDRGKDVNEPLFNFPEQEANHHD"
     gene            723..1040
                     /locus_tag="ACHA6Y_001551"
     CDS             723..1040
                     /locus_tag="ACHA6Y_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8987648.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            1284..2894
                     /locus_tag="ACHA6Y_001552"
     CDS             1284..2894
                     /locus_tag="ACHA6Y_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8987649.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            3051..3899
                     /locus_tag="ACHA6Y_001553"
     CDS             3051..3899
                     /locus_tag="ACHA6Y_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8987650.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            4130..4609
                     /locus_tag="ACHA6Y_001554"
     CDS             4130..4609
                     /locus_tag="ACHA6Y_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM8987651.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            4642..6870
                     /locus_tag="ACHA6Y_001555"
     CDS             4642..6870
                     /locus_tag="ACHA6Y_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8987652.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            6999..8642
                     /locus_tag="ACHA6Y_001556"
     CDS             6999..8642
                     /locus_tag="ACHA6Y_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8987653.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(8715..9260)
                     /locus_tag="ACHA6Y_001557"
     CDS             complement(8715..9260)
                     /locus_tag="ACHA6Y_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM8987654.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(9283..9768)
                     /locus_tag="ACHA6Y_001558"
     CDS             complement(9283..9768)
                     /locus_tag="ACHA6Y_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8987655.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(9758..10570)
                     /locus_tag="ACHA6Y_001559"
     CDS             complement(9758..10570)
                     /locus_tag="ACHA6Y_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM8987656.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(10709..>11818)
                     /locus_tag="ACHA6Y_001560"
     CDS             complement(10709..>11818)
                     /locus_tag="ACHA6Y_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8987657.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLQSLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         3808 a         2196 c         2364 g         3450 t
ORIGIN      
        1 gccactttgt ataagctata taccagtagg attagcttgc ggagtattgt tgcaaaaagt
       61 tggatttgat cctctcttat caggactgtt atcgattctg gtcttttccg gtggcgcgca
      121 gtttttagtt gcatctatgc ttacgacgca agcatcattt gcaacgacat tattgatggt
      181 tttcttttta gaattacgct atacattatt aggagcaagt cttgccggtt ttatgaaaaa
      241 agaaaagcgg acgtttttgg ctgtcttttc tcaatcactg aatgatgaaa actatgcggt
      301 gaactactta aagttttcaa ctgacccttc ctggaataaa agaaaggctt tatatgtcaa
      361 ttggttctca atgactgcat gggccggtag caatatgatc ggtaattttt tcggctcggt
      421 gattcgagta gatgctgacc ttgtccattt tgctttgacg gccatgttca ttttcatgtt
      481 tatcatgcag atgaaaagcg cactactgat ctttacaggt ttattctcag gcgtattagg
      541 ggtcgtattc atggtattat tccaaaatac tttcggattg attgcagcaa cagtcattgc
      601 ttcatttgcc ggatttatgc tggagaaggc attcaaaaaa gaaaaagcta agaaactaag
      661 agaccgcggt aaagatgtca atgagccgct cttcaacttc ccggaacagg aggccaatca
      721 ccatgactaa actatatttg ctacttttag ttgcttgtct gttcatcgtg gcatatatcc
      781 cgcgcttgtt cccgatgctt tattttactc atcgaaaagt gccatcctgg ttcagtgact
      841 ggatgaaata tgtaccggtg gcattatttg cggcgttagc ttttaaagat gttttcatta
      901 ctcatgaaca tctggatatt gcttggaata tcaaaatagc tgcaatgatt ctagtagcag
      961 gcgtggctta caaaacacgt tcgatggcgt tgtcagtttt gacaggactt gcgtctgtct
     1021 ttttgctgtc tatgctttaa actgttttat tttattaaaa acagagattg cgacataagt
     1081 tgttcaaact gatctatccg aactgcattt gcaacagtca attctggatg caagtttatt
     1141 cgtagggata aaaaagatta gtgttggctt atgatgcaag ctctttttta ttttaggaat
     1201 tagagaggga tttgtccttc tatgattttt tgctataata gggaagatgt ttattttatg
     1261 aaagaatagt tgagggaatt taaatgaata aaaaattgat gcaaggaaca ttttggctaa
     1321 cctttgctaa tcttctatgt aaagttttag gggtagtcta tttgattccc tggctaagta
     1381 tgatggggaa caatcaagat ggtatgcttg ctacgacatt gtataatgtt ggctatcttc
     1441 cttatggatt gttcctgatg cttggtacag tcggatttcc aaatgcgatt gcaaaaaaag
     1501 tagctgtagc cacaaaagaa ggagataacg ctgcttgtcg gctgattttt agaagtacga
     1561 tcaatatcat gtttgtcatc ggtatcgcct ctgctgcact gatgtattta tttgctcctt
     1621 tactagccgg aatcagtccg attgctaacg ttaataatgg gatactagct atcagaagtt
     1681 tatgcccatc tttgattgct atcccaattc ttagtgcaat gagaggatat ttccaaggaa
     1741 aaaatgatct gcggccttat ggaacttctt tgattatcga acaggctgtg cgagtgatcg
     1801 ttatcttggc tggtacttat tacttgcgcg tattgactga cggaaccata ttagaagctg
     1861 tattgatcag tacagtcgct tctttcttcg gtgggttagc tgcgattatc catatgtata
     1921 tcgtcggacg cagaaaagac ttcttcgaac ttaaagattt tttgatttcc agccgttatt
     1981 tcgaaagaga aaaccgatct gcctctgtct ctatcattcg ggaaacacta ccgtttattt
     2041 ttgtcggctc agtcattact atcgttcaaa tgatcgacca agtaacgatg aaaccactgc
     2101 ttcacttttt ccgtccagag atcgcagatc agcaactgga attcttattc agccgtgctt
     2161 ctgtcaatcc aaataaattg acattgatcc tgatctccat ggtaggaaca gtagctataa
     2221 gcagcttgcc aatattaagc acgatgaaga aaaaagatcg gatacaaatc gaaaaaacag
     2281 taggagatag tttttcgatt gcgttattga ttcttcttcc ttcattgact ggaatgtctt
     2341 tattagctgg accactatac acattgtttt ttggctatga tcctgaaagt gtcggatatt
     2401 tccaaatggc tttgctagct tctttgtttt tctctttatt cacgatccta tctacgatgc
     2461 ttcaatcttt gaatcaccat cgttttgcga tccgattaac tgtagaagca attatcctga
     2521 aagtcatctt ccaagtaatc ggattaggat tagttggcgg atacgggatg agcttatcaa
     2581 atggcttagc attcggtatt gtctttgttc gcggctatca ctttatgtgc aaagaatacc
     2641 gaatagcacc tttagcaaag atcagcaatt ttttcttaaa aactttccgc tccaccctga
     2701 ttatgcttgc agtatgtttt gccgttttct ttttgctgaa tcagcgattg tcaatggaat
     2761 caaaaagcta tgcaatgatt tattgtgtgg ctgtaggaag tattggcggc ttagtatttt
     2821 tattttctca attcggaaaa aatgggtttc gaatgttgaa aaatgttaaa catcatagca
     2881 gaagcaaaga ataggttaac taatcgtttt ttgctgtcac ttgaaaaacg gcaactcgta
     2941 tgattacgct tgtcgttttt ctattttcca attttagttt catatctaat cttacttatg
     3001 atatagttat aaggtctgga aaaattcttt gaaacgagga aattaaacac atgaaacttg
     3061 cagtagttac agatagttcg gcatatctgc cggaacgtat caagaaccat cccgacttat
     3121 tcgttattcc aatccctttg attatggatg ggaaaattta taacgaagga atcgatatcg
     3181 aagcggatga atattatgga cttttaaaaa acagtaaaga ttttcctact acttcacaac
     3241 ctgtcgtagg ggaagttttg ggtttatatg aggaattgaa agagaaaggg tatgacacga
     3301 ttttaagtat ccatctttct tcagggattt ctggctttgt caacacatta tttgcgatga
     3361 aagatgacat cagcggagta acggtcattc cttatgactc taagatcact agtatgccga
     3421 tgggacacat ggtagaagct gccttagact taaatgatga aggtcagtcc cttgatgaaa
     3481 tacttgcaca tatcgatcga atccgagata atacctacgc ttatctgatt gtagatgatt
     3541 tgaacaattt agtacgagga ggacgtttga caaatggtgc agcgctgatt ggcagcttat
     3601 taaaaatcaa accgatcctg acattttctg aagggaaaat tgttttattc gaaaaaatcc
     3661 gttcaagtaa aaaagcattt gctcgagctg aagaaattat tggtagaaga aataacgaaa
     3721 tacaaccgcc agtcaaactt tacgtgatac atgcaaataa tttagaagta gcgattgcgg
     3781 agaaagagaa attgcagaaa aaatatccaa atgcagaaat cgaaattggt catttcggac
     3841 cagtcattgg tacacattta ggtgaaaaag ccatcggcat tgccatttca gcgcaataat
     3901 tccttttatt caaaaaacat accaaaaata ttccaacatc atcttggact ttctacatgt
     3961 tactaatgag cgtgtaaaag aacgagatgg tgtttttatt tctatcaatg aagctctttt
     4021 tctttgcaat cctcacttca atcctctata atgtcgcata tagtttagta aatgagtaaa
     4081 ctactaattt acttatttac taaaaataaa aggatgagga gttaaaataa tggaaatacc
     4141 aacaaattta aaacataaac cagtaatcgt tgtagaagac tatgataaag ttgatggaag
     4201 aaatgcatta aatacggatg caaaagggct ttcactgggc cttgctcagt ggaatgatcg
     4261 tggaaaagtc gatatctcag gcaaggtatg gcgacatact ggagaaaaat ggtcacgaca
     4321 gtcggaagaa ttaccaatca cacgtatttt agatttagcg atcttagctg cacaaggaag
     4381 tttatacttc caagatgctt atcgttatga aaaattttat gatccagaaa atccagtcgt
     4441 agacatcatt ggattacaag ggaaccgaat gacggtagaa gtagatacca aaaatccaaa
     4501 gatcgatgaa gacatcattt tgtattacga tacactacaa aaagacggag aactgattgg
     4561 ggaacgattc cgtattttaa aacgtctttt agatgagcta ggatattaat agacagagat
     4621 gatcaaaagg ggaagagaag catggagaat ttctcactaa cagaaaaccg aaccttttca
     4681 cggctagaaa agcaaatgat ctggaagaaa ccgaaaagcc atcggacaag tgcagaagaa
     4741 tggcggatcg cctccgaaat caaagggaat tggcaagatc ctgaaatgaa aatcagtaac
     4801 gtacttttgg aaggagatgc cgggtcgggg aagacacaat tagccaaagc actatctgct
     4861 gatctgcaac ttccctatac aaaagtgacc tgttttgcag atatggacaa atcagatgtt
     4921 ttcggagcct tattacctgt agtagattct aataaggaac aagaccaaga attactagaa
     4981 gctatttacc aaacagatac acttgaagct gtactgacac tgatccagaa ccattacggc
     5041 attgatcctt tttctgctaa agaaaaatta gcagagctag ttcaaaggat cgaagctgat
     5101 tatccagatg caatccatta cagctattac ccttctgaaa tcgttcgagc gattgaaaaa
     5161 ggatatttat tagagattca agagccgact gtcatccgag atgcctctgt tcttgtggca
     5221 cttaactcag cgctcgaacc aaatgggatg ttgaatttac cgacaggaat cattcgtcgc
     5281 catccagatt gcgtcattgt gatcactaca aatcgaaatt accaaggaaa tcgcccatta
     5341 aatgaatctt taagagatcg gatgcaacat gcggaaaaaa tggatcttcc tgccttagaa
     5401 gtcatggcag aacgtgcgat ggcgaaaact atgatccaaa aacaagaact tctcttaaag
     5461 atggcagaga ttatccgttt gttggatgaa actgcaaaag ctaacgccat caaaggtgta
     5521 gcaggaatgc gttcttattt ttattgggca aatacctata agcaaggaca agatttactc
     5581 cagtcgattt atccaaaagt tttgtataaa ttatccactg attcagatga attggcaatt
     5641 cttgaacaag ctttgtctga cagtggattg ctactggaac ttcaggacat attgagaaaa
     5701 gaaaaatggg gtcatcctca atctgagcat acaaaaggaa gaaccattag cgccgaagaa
     5761 gcaaatgaac gaaacatcac tgaaaatgct gaagaaattc tgcgcttaga tgaaaagaaa
     5821 tcgttgtctg ctgaagaacc aaatgaggga gaagagaaac aagaagaaac aatagaaaaa
     5881 aacgagattc ctcctcaaat gcaaaaagaa cgcagtagcg atatcgaagg aaaaaatcaa
     5941 aaagaacaac aaacaggaga tgattctcaa gaaatgtctg cttctgacat ggaagcacat
     6001 gataaggaaa tcaagaagca actgaataaa gaagcgcgac aaatcatgaa ggaaacgatt
     6061 catgaaaaag aaggtctcat cgttcaccga ccagctttcc aaggaaaaac gccggaagca
     6121 cgtgagctac acatgcaagt aatgccaata gtagaatcac ttagccggca aattttagag
     6181 ttactggata atgaacaaac agagacgtac caaaaaggaa aatacgaagg tcagcggttc
     6241 aatgcaagca aagtagcata tggtgatttg agaaattttg acaaaaagaa cccgccacat
     6301 gagcaacctt ctttagcggt agctgtacgg atcgatgaat ctgggtcaat gatacgggat
     6361 gatcggatcg aagcggcaaa aaaagcagcg attgccattg ccgaattttc taagaaagtc
     6421 gatatcccat tgttgattta cggagataca gcagaccgtt cgtcaagaga aaagacttct
     6481 ttgttttctt ataaagaatt tgaagacgga tttcaatggt taaatgaaaa attagtcact
     6541 atgaaaccaa ggcaaaacaa tcgtgatggt gctgcacttc gtttagcagc agaaaaatta
     6601 aacagacaat ccgcaacaac caaactgttg cttaatatta gtgacggtca gccaaaagcg
     6661 ttgccggatt atacaggcaa aaaagcaaaa gaagatatcc agtctgtttt gaaagaatat
     6721 gaacgtcaag gaatcctatt tgtctccgca gctattggcc aagacaaaga agaaatcaag
     6781 tcgatctacg gtgaaagtcg ttttgtagat attactgact tgaatgaatt tcccaagcaa
     6841 atgattcaac tgattgcccg ctatctatga taaaataaag ctcaacgaac gcacttagca
     6901 gattcgttga gcttttcgtt tctagttacc ggtaacaagg atacttctaa aatagaatcg
     6961 gttataatca agagggaccg ttaaaggagt gaaacgatat gatacaagaa caaaaatggt
     7021 ggaaagaagc agtaggctat caaatctatc ctgctagttt caaagatagc aatggcgatg
     7081 gtcaaggaga catcaatggg atccgtgaaa aacttaatta tttgaaagat cttggtatcg
     7141 gctttatctg gatcaatccg atctatcaat ctccatttgt cgataatggt tatgacgtaa
     7201 gtgactatca agatatagaa gagagattcg gaacgatgga agattttgat ttattgctaa
     7261 aagaagcaca tgaattaggg atcaaagtga ttatggatct tgtcatcaac catagttcgg
     7321 atcagcatca atggtttgaa gaatcgaaaa aatctaaaga aagcccgtat cgcgattatt
     7381 acatttgggt cgacggggta gatggcaaag aaccgaataa ttggacatcg attttcggcg
     7441 gttctgcttg ggagtattct catgaaactg gacagtatta tctgcatgtt tttgcaaaag
     7501 aacaaccaga cttaaattgg gaaagcgaga aattgaaaga agaactattc aatatgatcc
     7561 gatggtggtt ggataaaggg atagatggat ttcgtttaga tgcgatttct catgtaaaaa
     7621 aagatgaata ttcagtaaaa gccacagaga accctttctc accctttcaa aatgtctcag
     7681 gaatcgaaga acatttgacc gatttgaaac atgtttttga agagtacgat atcatgactg
     7741 tcggagaagc aagtggtgtc actgcagaag aaggaccaca atgggtaggg aaagacggtt
     7801 atttcgatat gatattcgaa tttgatcata tccatatctg gcagcaagaa aaagaagggc
     7861 aattagatgt ccttaaattg aaacttgcat taagcgcttg gcaaacttca ttggatggta
     7921 tcggatggaa cgctttatat atggaaaacc atgatgttcc tcgtgccgtt tcagtgtttg
     7981 gagatactcg tcccgatttt tgggctatgt ctgcaaaagc aatcgctatg atgtatttct
     8041 ttttgcaagg tacgccattt atttatcaag gacaggaaat cggaatgacg aatatgccct
     8101 ttgaatcgat cgatcaagtt gatgcagtag attcgaaaag actttataaa aggctgcttg
     8161 cggaaggtaa gacaagagaa gaagcattgg atatcattcg tgaaacaacg agagacaaca
     8221 gcaggacacc catgcaatgg acttcggaac aatatgctgg tttttctaca catgaacctt
     8281 ggctgatcac taatccaaat accaaaacga tcaatgtaga acaacaagaa tatgaaccag
     8341 agtcggtcct gcaattctat aagaacatga tccgcattag acaaaccaat aaaggtctga
     8401 tatacggttc ttataaagaa tatcttcatg aacatccaca actttatgtc tatgaaagat
     8461 atttagaaga tgaacaatat cttattatgg taaacttaac agaatcactt gctgactatg
     8521 aacttccaaa agaagcagat cagtcctgga cattactctt gtcaaattcc tctagcggag
     8581 aatttgaagc taaaggcata ctagcacctt atgaagctcg gttatataaa acaaacaagt
     8641 aaaacttcct cgttaaagtt cattagttgg actttgcgag gaagtttttc agattgctct
     8701 gtcaattact catttcaata attcgctcta ggaccaccga tatatttagg acgagagcga
     8761 agggctgtca cgtgagcttg tccaagggtt ttcaaacgag gtctgacagg tacttggaca
     8821 tgtttgatat gcataccgat tgatgtgtca ccgatatcga taccggcttg tccaactacg
     8881 tgttccactt cgacaggatc attgaactgg tcaaaagcag caacagaaca agcacctcca
     8941 gcatgtagtg caggaacaac agagacaatt tcgaaatttt ttgcttctgc cactgttcgt
     9001 tctactacta atgctcggtt caaatgttca caaccttgga cagctagcgc aatttcttta
     9061 ggatccaaca actcttttaa cgtacggatg atccattgac caacttccgc actggaattt
     9121 tttccaatat gtcctccaac gacttcgctt gtgctgcatc ctaaaacaaa aagatcacct
     9181 tttttcaagt gtgcctcagc caatatgtca ttaactattt cagtcaattg gtcttttaat
     9241 tctttttctg acaacttcat tttctaaaac cttctttttc aattaatatt gcttccgtgc
     9301 ttgaatacgt tgaagacttg cagttatagg aaaggcaagt cccattccaa tcagattttg
     9361 gaagatgttt cctggtatag aagccactcc agccggccaa tcatataaaa tccaaccggc
     9421 gataccatag ccgatgatca taactaagct tcctaataaa agccctaatc cccacatacg
     9481 agtagaagat ttgctgaaag aaccagcaac atatccttgt aacccgtgga tcactaatga
     9541 aaaaactgcc cactgcggat atcctgaaat caaatcgatc aatagaccac tggaagcacc
     9601 aacaactaat ccacccgttg gaccataaag aaaagcagcg gtatagatcc cggcctcgca
     9661 taatgtcaca aatccattcg tagccggtac agggatcaaa attgttaatg aaagggcaac
     9721 tgtcaacgca atcagcatag catagatcgt taattgctta gttttcattt ctcattcctc
     9781 ctttttgaat accgccagac cagacacttc cggtatgatc gttcatcagc acgccattag
     9841 aaatacaatg ggcaacaaat tttttacttt ctgctactgc tttggtaaaa tctgccccta
     9901 acacaagatt cgctgttatt gcagaagaaa aggcacaacc ggccccgttt actgttgtag
     9961 tgtcgatttt tttatgagaa aatacctcat aatgatcaat tgaatagaga agatccacag
    10021 cttcatctcc agccatcctt gtgccgcctt tgatcacaat gttttttgtt cctgaagcag
    10081 tgagttcttt agcgagatgg atcatttctg ttaacgaaga gggaagagct tgaccagaaa
    10141 gtaaagcagc ttctgccaaa ttaggcgtaa tcatcgtaac aagtggaaaa agttcttcga
    10201 tgagctgcga aatatacaat tgattgatca cagcttcagt ttctttgaat gccaaaacag
    10261 ggtctaaaat gattgggata ttttgttttg tttttatgaa gtctcgaaca accatgattg
    10321 cttcacttga attcaataac ccaattttga taccatctaa tgaagtcatt ttatcaatcg
    10381 aaactaactg tcgtttaagc aattccaaag atatagattc cagcataaat cgctcattaa
    10441 tatctagtgc ccctatagaa gtcaaggcgc ttagtccgaa tagaccaaaa ttctcaaaag
    10501 tttttaaatc tgtttggatt cctccaccag caaatggatc agaaccgcca attgtcaaga
    10561 ttcttttcat aacattttcc tccatgaata agagtataac gaaattttca aaaaagaaaa
    10621 acagccaatt ggcatatttt tatctcatcc aatttaaaaa aacaaaaaga aaagaaagac
    10681 caaaatggat ctttctttcc ttaaatagtt aggacttttg tgattttaat tctttctcga
    10741 tttgaagccg ttgcactttc attgtagctg ttcgcgggat tgcatcaaaa tcacggatga
    10801 tagggacgtt caagcgagga agttcatgga cttgctccca ccatcgattc caatccattt
    10861 ctttatcagg agctaaagca ataatcggtt gtggagaatg atttttatcc cttacaatca
    10921 ctacttctgc caaaaaatct aacgaatcta ataaaaagtc ttcaatagct aatgtactat
    10981 tgatcgtttc gatcaaatcc acttgccgat ctttcaaaaa taggtggcca tgcttatcca
    11041 taaaaccgta atctccgcta tcccaccaat cgccataaac attttcctgg aaccgtgcat
    11101 cttccttgta ataagtcaaa gcccgtcctt tagacagtaa ttgtatatga ccatctgtgt
    11161 tttcaggtag gacattccca actgaatctg taatccgagc gcttgtcatg tcttccagtc
    11221 caacgcccat atctcttgca tcacttgttt tcaaactctg tagtgtatga gccttaagaa
    11281 tcattggtcc gcattcactt tggccatata cttgaagaaa taccgcatcc tgtgccatcg
    11341 acgttcgcaa aaatgtcttc attgtctggt tgtttatagc atcaaaagtg gagtgatagt
    11401 atcggatacc cgaaaaagct tctggatgct ccttagcagt aaaacgccac tgaacaaagt
    11461 tattcggatg ggtctctagt gcaattggcc ggtgagcttc caacatatga acaacttttg
    11521 aactttgtgc gtttgctaaa ggcatcatag gaaatcccat ggacatcaaa gaagagatac
    11581 caatattgaa tctggaatgc actggagaga tgtgaaaagc gattaatttc ttttccgaaa
    11641 tttttgtgaa aactgttttt tgccatttcg tccgccatcc catagaatga gcagaatgac
    11701 aaataagttt agggatacca gttgtaccag aagtatgggt catataagaa atttcatttt
    11761 tgtctaaaaa tgattgagaa acagacaaag caggttgttc cattaaataa agaatagg
//
LOCUS       DAYYHX010000078        11776 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000078 DAYYHX010000000
VERSION     DAYYHX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.079"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1172
                     /locus_tag="ACHA6Y_001561"
     CDS             156..1172
                     /locus_tag="ACHA6Y_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8987658.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            1313..2614
                     /locus_tag="ACHA6Y_001562"
     CDS             1313..2614
                     /locus_tag="ACHA6Y_001562"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM8987659.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            2660..3322
                     /gene="deoC"
                     /locus_tag="ACHA6Y_001563"
     CDS             2660..3322
                     /gene="deoC"
                     /locus_tag="ACHA6Y_001563"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM8987660.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            3343..3741
                     /locus_tag="ACHA6Y_001564"
     CDS             3343..3741
                     /locus_tag="ACHA6Y_001564"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM8987661.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            3807..4889
                     /locus_tag="ACHA6Y_001565"
     CDS             3807..4889
                     /locus_tag="ACHA6Y_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8987662.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            5101..6663
                     /locus_tag="ACHA6Y_001566"
     CDS             5101..6663
                     /locus_tag="ACHA6Y_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8987663.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            6656..7789
                     /locus_tag="ACHA6Y_001567"
     CDS             6656..7789
                     /locus_tag="ACHA6Y_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8987664.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            7789..8745
                     /locus_tag="ACHA6Y_001568"
     CDS             7789..8745
                     /locus_tag="ACHA6Y_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8987665.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            8844..10010
                     /gene="deoB"
                     /locus_tag="ACHA6Y_001569"
     CDS             8844..10010
                     /gene="deoB"
                     /locus_tag="ACHA6Y_001569"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM8987666.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            10034..10852
                     /locus_tag="ACHA6Y_001570"
     CDS             10034..10852
                     /locus_tag="ACHA6Y_001570"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8987667.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            10869..11576
                     /gene="deoD"
                     /locus_tag="ACHA6Y_001571"
     CDS             10869..11576
                     /gene="deoD"
                     /locus_tag="ACHA6Y_001571"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8987668.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         3724 a         2106 c         2738 g         3208 t
ORIGIN      
        1 ataaaataaa taagatacag aacttgtata gaaggctgcc aagtcggtag ataaaggagg
       61 tttttatcaa aaaacatact ttcctttttt ctctattctt ttcttggggg ttttggtaca
      121 ctgataggga atttagatag aagggagcca ataggatgaa gctgaccatc agagatatcg
      181 cagaaatggc aggcgtatca gtaacaactg tctcacagat tttgaataac aaaggaagca
      241 gatttagtga taagacaaga aaaaaagtgt tgtcgatcgt gaatgaacat cattataagc
      301 ccgattattt cgcttcaaat gtgatcaatc gacattcaaa gaccatcgga atgatcgtcc
      361 ctgatgtcac tgatttcttc ttctccaaac tgatcgaagg tgtagagagc tatttgaatc
      421 ctttgggcta tgtgatcatg ttatgcaatt ctcgtcatag ccaagaaaat gagatcaagt
      481 atttacaaga gctttcccac cgctcagtgg acggaatctt gctggccaca ccaaatattt
      541 tacctgagaa atatgcatta gacagcggtt tttatcaaaa aatgccaatc attttaatcg
      601 atcgcggatt gaatcgtcgg gataacggac ggcttatcgt aaaggaatat gagggcgcct
      661 atcaagctgt ttcgctgttt attgaaaatg gccatacgaa gatcggcatg ctgaaggaaa
      721 ccactggata ctatcaatta gaagaacgtt tcaacggata tcgtcatgcg ctgaaagatc
      781 atggcctgcc ttttaatggg aaatttgttg aacatggaga gttgactgtc caaggtggat
      841 atactgcatc aaaaaaatta ctcaaacata aagatatcac ggctattttt tgcgggaatg
      901 atgccatggc gatcggatgc tatcaggcga tcgatgaatt gggcaaaaaa atcccagaag
      961 atatctcagt cataggattc gatggattaa agctgtcaga atacatgatt ccgcaattga
     1021 cgacagtgca gcagccaagt tttgatattg gattttatgc tgcacgtttc ctgatcgata
     1081 cgatcgaatt tcctcaaaga aaagttccga acaaagtgtt cgaaacaaaa ttaattattc
     1141 gtgagagcgt aaaagtattg acaaagggct aactcggcgg tatattgctt atgtaagcat
     1201 ttacaaaggc tagttagtgc tttttttatt tttagtaaac cagtttacta aagtggttta
     1261 cctttcctaa aaataatgta taataccgaa tgaagacaag gaggatcagg caatgcgaat
     1321 ggtggatctg attgaaaaaa agagggatgg aaacgagctg tcgaaagagg aaatcgagta
     1381 catcgtcacg aactatacga atggaaagat tccagattat caagtcagtg cattattgat
     1441 ggcaattttt tatcaagaca tgacaaatga agaaatcacg aacttaacat tggcgattgc
     1501 taattctggt gatgtcatcg atttgagttc tcttgaagga ataaaggtag ataagcattc
     1561 gacaggtggc gttggtgaca cgaccacatt gattttagcc cccctagtag ctagtgtcgg
     1621 agtgacagtg gctaaaatgt ctggacgcgg tctaggttac acaggtggta cactagacaa
     1681 gctagaagca attcccggat ttcagatcga gctttcggat gaagaatttg tacgcattgt
     1741 taatgaaagt aaagtagcgg taattggtca atcaggaaac ttggcaccgg cggacaaaaa
     1801 attgtatgcc ttgcgtgacg tcacagcgac cgttgattct cttccgctga ttgccagttc
     1861 gattatgagt aaaaaaatcg cggcaggagc agatgcaatc gttttggatg tgaccactgg
     1921 agacggtgct ttcatgaaga acatcgaaga tgcaagacgt ctggcaaaga ccatgacgag
     1981 cattgggaaa ctggctaacc gagaaacagt ggcggttatc tcagatatgt cagaacctct
     2041 tggcgaagct atcggaaata gtctagaagt tgtcgaggca atcgaaacat tacaaggaaa
     2101 tggtccagaa gatctagtag aaatgtgcta cgccttagga agccaaatgg ttgttcttgc
     2161 tggcaaagct aagacaaccg atgaagcaag aacgttgctt caagaagctc tggaatcagg
     2221 taaagcgtta gcaaaattca aagaaatgat ccaaaatcaa gggggagacc cagcgatcgt
     2281 ggaacatcca gaacgaattt tgactgcacg ctacacaatg gagcttcctg ctaaacaaag
     2341 cggagttgtg tcaaaaatcg tggcaaatga attaggaatt gctgcaatga tgctaggtgc
     2401 tgggcgtaaa accaaagaag aagatattga tcacgctgtt ggattgaaac tgcataaaaa
     2461 aattggtgac actgtgacaa aaggtgagtc actattgacg atctatagta acgacgaaga
     2521 aatcacttct gtcattgaat tattatataa aaatattgaa attggtgaat cggcaatgaa
     2581 gccaacgttg attcatgaca tcatcacaga ataaaggaaa aaagcggact ttaccgtttt
     2641 aaagaaaagg ggaaaagaaa tggaactaaa ccgaatgatc gaccatacta ttttgaaagc
     2701 agacgcatct aaagaagctg tgatgcaaat tatcgaagaa gcaaaaaaat accattttta
     2761 ttctgtctgc atcaatccag catgggttgc gctagcgaaa gaacaattac aaggtgagcc
     2821 agtagccgta tgtacagtaa tcgggtttcc tctaggagca aacacacccg agacaaaagc
     2881 atttgaaaca acagatgcca tcaataacgg tgcagatgaa gtcgatatgg tcatcaatat
     2941 cggtgctttg aaatcaaagg atgatcaaca agtccaaaaa gatatcgaag cagttgttga
     3001 agcagcaaaa gatcgtgcgc ttgtcaaagt tattattgaa acatcgttat tagatagaga
     3061 agaaatcatc cgagcttgcg aaatagcaaa agctgcaggc gctgattttg tcaaaacatc
     3121 aactggtttc tcaacaggcg gagcaaaggt agaggatgtc cgtttgatgc gtgaaactgt
     3181 aggaccagaa atgggtgtca aagcttccgg cggtatccat aacgaagaag aagcaatggc
     3241 aatgatcgaa gctggtgcta caagaatcgg aacaagcgca ggagtagcaa tcgtatctgg
     3301 ttcaactggt gaaggctatt aattttttca aaggagaagc tcatggcaga actcaaacaa
     3361 gaatggatca acacagctat tgaagcgtta gataaagcct atgttcctta ttcccatttt
     3421 ccggtaggcg cttgtctcgt aacggaatca ggcaagattt atcaaggcat caacattgag
     3481 aatgcatcat ttggattaac gaattgtgcc gagcgcacag cttttttcaa agcagtatct
     3541 gaaggagaaa aaacatttac gcatctagtc gtagcggggc acacccccga tcctatctct
     3601 ccttgtggtg catgtcgaca agtaatggca gaattctgtt cccctgatat gccggttacg
     3661 ctagtaggag acaatggtgt gacgaaggca acgaccgttc gtgcactgct gccttatgca
     3721 tttactgaaa aagatctata actcatgttc attcagtcag ctgactgttg aaaaataaaa
     3781 caattatttt aggaggcttt acagaaatga aaaaagcaaa attatttggt ttaggtgctg
     3841 tcgcactagc agcagggtta tttttaggtg catgcggcaa caacggctct actgattcat
     3901 ccggtggcaa aagctcatca gacacaacaa ctgcagcatt gatcacagac acaggtggtg
     3961 tagacgaccg ttcattcaac cagtcagctt gggaaggact agaaaaatgg gggaaagatc
     4021 aaggactttc acgaggaaat gatggcttcc aatatttcca atcttcaaat gaatcagatt
     4081 acatcccaaa tattgaccaa gcattgaatg ctggattcaa aacgattttt ggtattggtt
     4141 ataaactaaa accagcgatc caagaacaag caacaaacaa tacaggtacg aactttgtta
     4201 ttatcgatga tgtcatcgat ggattagata acgttgtatc tgcaactttc aaagacaacg
     4261 aagcttctta tcttgcaggt gttgcagcag cttatacgac tgaaacaaac gtagtcggct
     4321 ttatcggcgg tgtaaaaggt gaagtaatcg atcgttttga tgctggattc aaagccggtg
     4381 ttgatgcagg tgcgaaagaa ttaggaaaag aaatcaaagt attgaatcaa tatgctggtg
     4441 acttctctgc tccagataaa ggacgttcaa ttgctcaagg tatgtatgca caaaacgcag
     4501 atatcatctt ccatgcttca ggcggtacag gtaacggtgt attccaagaa gcaaaatcat
     4561 tgaacgaatc aggcgacaaa aaagtttggg ttatcggtgt tgaccgcgac caatcagacg
     4621 aaggcgaata cacattaaac ggcgaaaaga aaaacttcac attgacatct acactgaaag
     4681 cagttggaac agtagtagaa gatttggcac aaaaatcagc ggacggtaaa ttccctggcg
     4741 gcgaacacac tgtttatgga ttgaaagaag acggtgttgg tctaacagaa ggacaacttt
     4801 ctgacgaagc gaaaaaagca gttgatgaag caaaagaaaa aatcatttca ggtgacgtaa
     4861 aagttccaga aactcctgaa gaaaactaaa accatttcta tagccttgtt agacacatgt
     4921 ttgacaaggc tatggtttga ttagaacaag aattgttcat ttatccaagc tgccttgtga
     4981 cagtttggat aagggaacaa ctccctgaag taatgtttgt ttcaaaagaa gaacgctcac
     5041 taagcgtgaa tcttagtcaa tcgttgccct agatagaaat cgttaggatg tgaaaaaaag
     5101 gtggttcaag aaaatattgt catagaaatg cagaatatta ccaaagaatt tggaacattc
     5161 aaggcaaatg acaacatcaa tttacaagta aaggcaggag aaattcatgc gttgcttgga
     5221 gaaaacggtg ctggcaaatc tacattgatg aacgtgcttt ccggattatt agagccgaca
     5281 tcagggaaaa ttttgatgcg tgggaaagaa gtaaagatca caagtccgac caaagccaac
     5341 caattaggga ttgggatggt ccatcagcac tttatgcttg ttgatgcctt tactgtaaca
     5401 gaaaacatcg tgttgggaag cgagcctagt cgcgcaggga tgcttgacca taaaaaagca
     5461 cgaaaagaga tccaaaaagt ttctgaacag tatggattgt cagtcaaccc agatgcttac
     5521 gttcgtgata tttcagttgg aatggaacaa cgggtagaaa ttttaaaaac actttaccga
     5581 ggagcagatg tactgatttt tgatgagccg acagctgtat tgacccctca agaaattgat
     5641 gaattaatcg tgatcatgaa ggaattagtc aaagaaggca agtcgatcat tttgattacg
     5701 cataagttag atgaaatcaa agcagtagct gaccgctgta cagttatccg ccgtggaaaa
     5761 ggaatcggta cagtcaacgt taaagacgta acctcacaac aattagccga tatgatggtt
     5821 ggaagaacgg tttcattcaa aacgatgaaa aaagaagcga agcctcaaga agtcgttttg
     5881 tctattgaaa atctagtggt gaaagaaaat cgtggattag aagccgtgaa aaacttgaac
     5941 ttagaggttc gtgctggcga agtacttggt atcgctggaa tcgatggaaa cgggcagtcg
     6001 gagttgatcc aagctttgac tggtttgcga aaggcagaaa gcggacatat caagctaaaa
     6061 ggggaagaca tcactaataa aaaaccccga aaaatcactg aacatggtgt aggacacgtg
     6121 ccagaagacc gtcataaata cgggttagtc ctagatatga cattatctga aaatatcgca
     6181 ctacaaacct atcatcaaaa accttacagt aaaaacggca tgctgaatta ttcagtaatg
     6241 aatgaacatg ccagagaatt gatcgaagaa tatgatgttc gaacaacgaa tgaacttgtt
     6301 cccgcaaaag ctttatcagg cggaaatcag caaaaagcaa ttatcgctcg tgaagtcgac
     6361 cgagatcctg atctgttgat tgttgcaaat ccaactcgtg gactggatgt aggtgcgatc
     6421 gaatttattc ataaacgtct gatcgaacaa agggacaaat acaaagcagt gttactgatc
     6481 agtttcgaat tagaagaaat tttaaatgtt tcggatcgta ttgctgttat ccatgaagga
     6541 gaaattgtcg gtatcgttga tccgaaagaa acatctgaaa atgaattagg tctattgatg
     6601 gcgggctata ccttagaaga agcacgaaaa gagttagaaa agggggctgg cgcacatgaa
     6661 tgatcgcagc gaaaaattcc gtaatttatt agtgcctgta ctttcagttg ttttaggatt
     6721 gatcttaggc gcgatattga tggctgcttt tggttttcag cccatccaag gttattcttc
     6781 tatgctagat gcagcccttg gttcgcaaag agggataggt gaaacgttaa ggcaagcaac
     6841 gccgttgatt tttacagcat taggtttttc tgtagctaac tcagctgggt ttttcaatat
     6901 tgggctttct ggacaagcac tttgcgggtg gatcgtcagt gtctgggtgg cacttgcgtt
     6961 tccagatctg ccaaaagttg ttttgcttcc gttatgcgtg atcttaggtg ccttagctgg
     7021 tgcgatggca gcggctgttc caggtttact tcgagcattt tttggaacaa gtgaagtgat
     7081 cgtaacaatc atgatgaact atatcttatt atacgtaagc acatttgctt tacatgaagt
     7141 gatgcctgaa agctatcgct caagtttgga ctcttccaat atgatcagtc aaaatgcctc
     7201 actgcggtta ccatggttaa cacaagcttt tggcggctct cgcgtaaact caggattatt
     7261 tttagcaatc atcgcgctta ttttcgtttg gctgctgatg aagaaaacga ctttaggatt
     7321 tgaaatccgt tctgttggat tgaatccttt tgcttctgag tatgcaggaa tgagtagtaa
     7381 acgtacgatc gttttgtcga tggtcatttc tggcgctttg gcagggcttg gcggtgtggt
     7441 tgaaggatta ggaacgtatc aaaacttctt tgttcaaacg acttcgctat ctattggatt
     7501 tgacggaatg gctgtttcct tgcttggttc tggatcagca atcggtattc ttttatcagc
     7561 gatcttgttc agtattttga agatcggtgg acttggcatg cagacaggag caggtgttcc
     7621 gtttgagatc gtcaatgtat cgattgcttt gattatcttc tttgtcggga tcaacttcct
     7681 gattcgtttc gtcatggcta aagtcttcca agggaaaaaa caagaagaga tcgttgcgac
     7741 aatagaatcg aaaccaacga atcaaaacca tgaagggggc gaactataat gtcgactatc
     7801 gaattaattg ccttgatcct tacttctaca ctggtttatt caacaccgct aattctaacg
     7861 tcactaggcg gaactttttc tgaacgaagc gggatcgtaa acgtagggct agagggaatc
     7921 atggtcatgg gtgcttttag ttccatcgtt ttcaacttga cgatgagtcc atcacttggt
     7981 tctatgacac catggatcgc tattttagtg ggtggcgtcg taggtatcct attttctctg
     8041 attcacgcag tagcaacagt cagtttgcgt gctgaccata ttatcagcgg gacagtaatt
     8101 aatctgatgg cacctgcttt aggtgttttc ttgatcaaag catggtatgg taaaggtcaa
     8161 actgataaca tcacgcagaa tctggggtat ttttctttcc caggattagc agatattccg
     8221 gtcattggac caattttctt taaaaataca ttcttacctg cttattgtgc tattttgatt
     8281 gctgtcctag cttgggtgat catcttcaag acgaaattcg gcttgcgcct ccgttcagtt
     8341 ggtgaaaatc ctcaagctgc cgacacatta ggtatcaatg tatatgctat gcgttattca
     8401 ggcgtactga tttctggctt gttaggcggt atgggcggtg ctgtttttgc tcagtcgatt
     8461 tccggtaact tttcagctgg tacgatcgtt ggacaagggt tcatctccat ggctgctatg
     8521 atctttggta agtggaatcc tttaggggca atgggtgcct cattgttctt tggttttgct
     8581 caaagcttga gtatcattgg cgcacaatta ccagttattt cttcgattcc gccagtattg
     8641 ctgcaaattg ctccatattt gctgacaatc attgtgctgg ttgttttctt agggaaagct
     8701 tctggtccaa aagcaaacgg gaagaactat atcaaatcaa aataatcttt tagcgaataa
     8761 aaagggtggg ttacgggaag tgtttttcgt ctcgcccttt ttgttttaaa ggcaataaaa
     8821 ataaaaagaa agaaggaata atcatgttta aacgcgtaca tctgatcgtc atggattcag
     8881 taggaattgg agaagcacca gatgccgata agttcggtga tgttggttct gatacattag
     8941 gacacattgc gaaagaagca ggattgaacg ttccacattt agagcagctt ggtttaggaa
     9001 cgatccgtcc tcttgaaggt gtggaatcag tgaaagatca tcaaggctat gcgacgaaat
     9061 tagaagaagt ttctgtaggg aaagatacga tgacaggcca ctgggaaatc atgggattga
     9121 atatcaaaac tccatttcgt gtgtttccta atggattccc tgatgatctg atcaaacaaa
     9181 tcgaagaatt ttctggccgt aaagtcgtat gtaacaaacc ttatagcgga acagctgtta
     9241 ttgacgatta cggtgaacac caaatgaaaa caggcgattt aattgtatat acttctgccg
     9301 atcctgtcct tcagatcgcg gcacatgaag aaatcattcc tttagaagaa ttatatcgga
     9361 tttgccaatt cgttcgtgat ataacaaaag atgatccgta tatgattggc cgaatcattg
     9421 cacgtccgta cgtgggtgaa ccaggagact tcacacgaac aagcaatcgc catgattatg
     9481 cgttgaatcc attcggacac acagtccttg attctttgaa agaagcagga aaggacgtga
     9541 ttgctgttgg aaagatcaac gatattttca acggacaagg aatcactgaa tctgtccgca
     9601 cgaaaagcaa tatggatggc gttgaccagc tgctgaaagt tatgaaaaac gatttttcag
     9661 gaatcagctt taccaattta gtagattttg atgcattata cggtcatcgt cgggatgtag
     9721 tcggctatgc gcatgcgatt gaagaatttg acttacgaat tccagaattg cttgaagcga
     9781 tggaagaaga cgacttgcta atgattacag ccgatcacgg aaacgatccg acgtttactg
     9841 gtaccgatca tacgcgagaa tatgtaccgt tattggttta cagtaaaaaa atgaaagaac
     9901 aaggggcatt gcctcaagga tattattcag atatttcagc aacgattgct gagaacttta
     9961 gtgtttcttc gactgaaaat ggcgaaagct tcttgaaatt attaaaataa tctttttaaa
    10021 taggagaaag caaatgacca agttaagcga gatgttacaa gaaacgaccc agtttatcct
    10081 tgataaagga gtaaaagaaa ttgattttgg tttgattcta ggttcaggat taggtgaatt
    10141 agcagatgaa atcgaagatg ctgtaatgat ttcttatgat gagatcccgt tctttcctgt
    10201 ttcgacagta gttgggcatg caggaaagct agtttatggg tctctggcag gcaagaaagt
    10261 gttagccatg caagggagat tccactacta tgaaggtcat tccatgcaaa cagtgaccta
    10321 tccagtacgt gtgatggccg gactaggggc ccattcagtc attgtgacta atgcatgtgg
    10381 tggtgtcaac gaatcatttg taccaggaga tttgatgctg attaccgatc atatcaattt
    10441 tactggtgaa aatcctttga tcggtccgaa tgaagatgca atgggcccta gatttccaga
    10501 tatgagtcaa gcttatacgc ctgactatca agcaacagca aaagcagcag cagaaaaact
    10561 aggtttggat cttaaagaag gcgtatatat gggctattct ggaccaactt atgaaacgcc
    10621 tgcggagatc cgaatgtctc ggacaatagg agccgatgca gtagggatgt cgactgttcc
    10681 agaagtcatc gtagcagctc acagcggatt aaaagtgtta ggaatctctt gtattaccaa
    10741 cttagcagca ggtatgcaag cgaatctgag ccatgaagaa gttgtagaaa caactcagag
    10801 agtcaaacaa tcttttaaag cattggttaa agaaacattg acattattgt aataggagga
    10861 acaataaaat gagtgtgcat attgaagcga aagaaggcga gatcgccgat aagattttac
    10921 ttccgggaga tcctttgcga gctaaatata ttgcggagac ttttttggaa gatccagttt
    10981 gttacaatca ggtacgcggt atgttaggct ttaccggcaa atataaagga gaacgtattt
    11041 ctgttcaagg aacaggaatg ggaatgccat cagccaccat ctatgcccat gaattgatcc
    11101 agtcgtatgg tgtaaagaaa ttgattcgag taggtacatg cggtgcattg tcaaaagacg
    11161 tccatgttcg tgatttagta ctagcacaag gtgcagcgac cagttcttcg atgatcgaaa
    11221 aaaatttcca agcctttcat tttcctccaa tcagtgattt caatctatta ctgaaagcat
    11281 atgagatagc taaagaaaag ggctatacgg ttcatgtagg aaatgttctt tcggaagatt
    11341 ctttctacaa agatgatttg acagagacgt tccaattagc tgaacttggc gttttaggag
    11401 tggaaatgga agcagcggct ctttattact taggcgcgaa ataccatgtg cagacattaa
    11461 gtttgatgac cgttagcgac catttgatca ccggtgaaga aacaacagct gcagaacgtc
    11521 aatctacgtt caatgaaatg atcgaagtag gattggaaac agcgatagca gaataagaag
    11581 gaaatgaaaa agctgccctg cattaagtaa taagaacatc caagcaaaaa tagctcctca
    11641 tgtttttgac ttggctggac ttattattgc aaatgtaagc ttttgaacac cgtttaccaa
    11701 gaggttgtga atctgccgtt taactctgag tcacaaggaa caatttcaaa aaacagcttt
    11761 tcatgtttga tgaata
//
LOCUS       DAYYHX010000079        11701 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000079 DAYYHX010000000
VERSION     DAYYHX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.080"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..1450
                     /locus_tag="ACHA6Y_001572"
     CDS             122..1450
                     /locus_tag="ACHA6Y_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM8987669.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            1652..2485
                     /locus_tag="ACHA6Y_001573"
     CDS             1652..2485
                     /locus_tag="ACHA6Y_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM8987670.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2501..3238
                     /locus_tag="ACHA6Y_001574"
     CDS             2501..3238
                     /locus_tag="ACHA6Y_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM8987671.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3393..4361)
                     /locus_tag="ACHA6Y_001575"
     CDS             complement(3393..4361)
                     /locus_tag="ACHA6Y_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM8987672.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            4593..5426
                     /locus_tag="ACHA6Y_001576"
     CDS             4593..5426
                     /locus_tag="ACHA6Y_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM8987673.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5460..6299
                     /locus_tag="ACHA6Y_001577"
     CDS             5460..6299
                     /locus_tag="ACHA6Y_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8987674.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6325..6885
                     /locus_tag="ACHA6Y_001578"
     CDS             6325..6885
                     /locus_tag="ACHA6Y_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8987675.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7084..8805
                     /locus_tag="ACHA6Y_001579"
     CDS             7084..8805
                     /locus_tag="ACHA6Y_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM8987676.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            8970..10112
                     /locus_tag="ACHA6Y_001580"
     CDS             8970..10112
                     /locus_tag="ACHA6Y_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8987677.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10128..11339
                     /gene="thiI"
                     /locus_tag="ACHA6Y_001581"
     CDS             10128..11339
                     /gene="thiI"
                     /locus_tag="ACHA6Y_001581"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM8987678.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4003 a         1946 c         2466 g         3286 t
ORIGIN      
        1 ttcttcatga agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt
       61 gtatttctaa attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg
      121 gatgaaagct attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat
      181 caaacagcag gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa
      241 tgtttcagca catgtggaga atacaccggt attttctata gatttggcta cggggaaagt
      301 ggctaatcaa atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca
      361 taagatgctg aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt
      421 ctgggataaa tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga
      481 ggcaatcgat agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca
      541 atgggatatg atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc
      601 ggaaagtgcg aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg
      661 taaagatgca gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga
      721 agaaaagaaa gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac
      781 acctccaaaa gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg
      841 aggactgaca ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt
      901 tagcgtgatc aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat
      961 gctaggaaat gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt
     1021 caaaaaatta gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt
     1081 aggtcaatct tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga
     1141 tctatttgat acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct
     1201 gatgacccat gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg
     1261 gaaagtcgaa aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga
     1321 tgcctggatg gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga
     1381 gcaattaaat gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc
     1441 tttagcatag acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta
     1501 agccagctct ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat
     1561 tctaaataaa ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag
     1621 tagaataaaa ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct
     1681 gaaattaaat ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat
     1741 aaaaaccgaa catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt
     1801 tgaagcagaa gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta
     1861 tatgctaggt gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa
     1921 aggggatcaa cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca
     1981 atttggtttt cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga
     2041 agattggtgg acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga
     2101 aacgaatcga tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt
     2161 gaaatcggtt gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg
     2221 taatgtgttc tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg
     2281 gaatcgggaa caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt
     2341 agatgcttat caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta
     2401 ccagttatat tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt
     2461 agatcaatta ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa
     2521 aaagcatgaa gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat
     2581 gttaggagta gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga
     2641 attcgtcaat agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg
     2701 tctccctaga gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt
     2761 tttctgttat cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga
     2821 atctgttttc gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat
     2881 cattgctcct tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa
     2941 gattgattcg ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca
     3001 gcatgttaat aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt
     3061 gactacgcat gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa
     3121 tgagatcgaa agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga
     3181 aatccgaatg caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa
     3241 aagaggttgt gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt
     3301 tctcaaattt ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc
     3361 tctttttaag tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct
     3421 tcacccgaca aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta
     3481 atactgttac agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca
     3541 taaacaggct ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg
     3601 acaagaagct tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct
     3661 tcgattacca aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc
     3721 ccagcatatg ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt
     3781 gtttctaaaa gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc
     3841 aatgtaggtg ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca
     3901 gaatggatct catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt
     3961 gcgacacgaa tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa
     4021 cgcattgccc cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa
     4081 gcagtttctt ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca
     4141 taagcggaca agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag
     4201 ggaagattga agatatcttc aactaataat tctacatgct tggggattgt gatttctgca
     4261 tctaataatg ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg
     4321 gcgatcgttc cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga
     4381 ataattttgc atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt
     4441 gaagaataac gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga
     4501 ctcaaccttg acctcctaat acaatggagt atctggttga gtttctttta caagaaagca
     4561 gccaaggaaa tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta
     4621 aagaacatga caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc
     4681 tacccttagt ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga
     4741 attggataag tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca
     4801 gcggtcaagg agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt
     4861 tagatgttat gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc
     4921 cctcatcatt caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt
     4981 ttgagggtta tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc
     5041 ttgaacgtac aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc
     5101 cacagttgct gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc
     5161 tttttcgatt ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta
     5221 aaaaaagaac gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca
     5281 aagtagttga ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg
     5341 atgcggctga aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca
     5401 atccaaccca aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta
     5461 tgatcaaagc tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt
     5521 tggaatcaac aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag
     5581 caacggggcg cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt
     5641 atgtcgtgta taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt
     5701 ttgaacaaaa agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg
     5761 tttttggtgc gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt
     5821 ttatcaagcg actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa
     5881 tcctccaggt ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa
     5941 aagaaccaat ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac
     6001 atcgctttcc cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa
     6061 aaggtggttc aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg
     6121 aagaaattat ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa
     6181 ttggagtggc catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc
     6241 aatcaaatga agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt
     6301 caatgaaaga aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca
     6361 ttccacaaaa cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa
     6421 gctcatacac gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca
     6481 cctaataaag aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca
     6541 gaagaaaaaa tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat
     6601 gcactgacag atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct
     6661 gatcgaatat tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa
     6721 cctcattatg accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat
     6781 gctccagaac ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag
     6841 aagaaatccg ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat
     6901 ttttgttatc tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg
     6961 aaaatagtat gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat
     7021 ttatgccaag cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca
     7081 taaatgggaa ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc
     7141 gtctttttgg ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa
     7201 aaacgcaagg aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa
     7261 atgcatctgg ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac
     7321 atatctactt cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat
     7381 gaaagttttc ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac
     7441 caaatggaga aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa
     7501 gaacgcaact cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa
     7561 gtcagagaag acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa
     7621 gtgatcgaaa aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa
     7681 gcccgcgagg ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa
     7741 aaaattccac cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc
     7801 agtgatactt acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct
     7861 gaagatatcg caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca
     7921 gaagttgaaa cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat
     7981 gatatcttag aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt
     8041 gcagagtata tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac
     8101 acatctcaaa gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc
     8161 gaagtggaag aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa
     8221 attccttatt cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc
     8281 gaaaagcaac aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa
     8341 gcgcaagaaa agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa
     8401 aaacaacgtt tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac
     8461 cgtattgaag aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat
     8521 cgtttagttg ctctatgtga agaagatcta gaattattag ataagaagac acatgattta
     8581 gtagatgcag cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca
     8641 cacgaagaag tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac
     8701 tatcaagaag cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc
     8761 aaacgcatcg aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt
     8821 aaaccagcaa gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa
     8881 attgaaacaa gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag
     8941 aaaagaattt caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc
     9001 caatctttcc gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc
     9061 catcaagctt gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc
     9121 agatcgctga tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag
     9181 aaggagataa ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata
     9241 tgatcatttc aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat
     9301 taggaatcga agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat
     9361 tgaaagaatt aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag
     9421 taggatcgat tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc
     9481 atttccatgt agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag
     9541 aaagagttga ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt
     9601 tcatttattg gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa
     9661 gaaatcagcg aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac
     9721 gtttatcatt agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc
     9781 taatcaatgc gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg
     9841 caccgcatat tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct
     9901 tagaagaaaa acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg
     9961 caagcagtac attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa
    10021 tcagcttaga tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc
    10081 agctttataa gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg
    10141 aaattatggt ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca
    10201 tgcaactagc acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg
    10261 ctgatcggga ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa
    10321 aactagcaaa aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg
    10381 tcgaagtttt gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa
    10441 catttaaaat tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga
    10501 atcaaacatt aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc
    10561 aaccagatat tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa
    10621 cgatcaaggg agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt
    10681 ctggggggat tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg
    10741 aagctgttca ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag
    10801 atctaacagc caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta
    10861 ccgagattca agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc
    10921 gtcgaatgat gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt
    10981 tgaatggtga atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca
    11041 atgaagtaac gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca
    11101 ttgaagtagc agaacagatc gatacattta atctttctat ccagcctttt gaagattgct
    11161 gtacgatttt tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat
    11221 atgaagagcg tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg
    11281 aagagataca agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag
    11341 aggttatgaa aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc
    11401 atgtatttta ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa
    11461 gaagttatgg aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat
    11521 caagctacag ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg
    11581 acagaagtgg gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt
    11641 tgtgaaattt cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt
    11701 g
//
LOCUS       DAYYHX010000080        11660 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000080 DAYYHX010000000
VERSION     DAYYHX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.081"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACHA6Y_001582"
     CDS             complement(132..509)
                     /locus_tag="ACHA6Y_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987679.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACHA6Y_001583"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACHA6Y_001583"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM8987680.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACHA6Y_001584"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACHA6Y_001584"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM8987681.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACHA6Y_001585"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACHA6Y_001585"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM8987682.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACHA6Y_001586"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACHA6Y_001586"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM8987683.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACHA6Y_001587"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACHA6Y_001587"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM8987684.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACHA6Y_001588"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACHA6Y_001588"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM8987685.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACHA6Y_001589"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACHA6Y_001589"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM8987686.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACHA6Y_001590"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACHA6Y_001590"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM8987687.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACHA6Y_001591"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACHA6Y_001591"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM8987688.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACHA6Y_001592"
     CDS             10661..11575
                     /locus_tag="ACHA6Y_001592"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM8987689.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
BASE COUNT         3834 a         1972 c         2733 g         3121 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc
//
LOCUS       DAYYHX010000081        11565 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000081 DAYYHX010000000
VERSION     DAYYHX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.082"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..491)
                     /locus_tag="ACHA6Y_001593"
     CDS             complement(114..491)
                     /locus_tag="ACHA6Y_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987690.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(791..1672)
                     /gene="galU"
                     /locus_tag="ACHA6Y_001594"
     CDS             complement(791..1672)
                     /gene="galU"
                     /locus_tag="ACHA6Y_001594"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8987691.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1715..2734)
                     /locus_tag="ACHA6Y_001595"
     CDS             complement(1715..2734)
                     /locus_tag="ACHA6Y_001595"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8987692.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3367..4371)
                     /locus_tag="ACHA6Y_001596"
     CDS             complement(3367..4371)
                     /locus_tag="ACHA6Y_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8987693.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4384..5433)
                     /locus_tag="ACHA6Y_001597"
     CDS             complement(4384..5433)
                     /locus_tag="ACHA6Y_001597"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8987694.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5450..6271)
                     /locus_tag="ACHA6Y_001598"
     CDS             complement(5450..6271)
                     /locus_tag="ACHA6Y_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8987695.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6258..7037)
                     /locus_tag="ACHA6Y_001599"
     CDS             complement(6258..7037)
                     /locus_tag="ACHA6Y_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8987696.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(7054..7524)
                     /locus_tag="ACHA6Y_001600"
     CDS             complement(7054..7524)
                     /locus_tag="ACHA6Y_001600"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8987697.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7536..7955)
                     /locus_tag="ACHA6Y_001601"
     CDS             complement(7536..7955)
                     /locus_tag="ACHA6Y_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8987698.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(8036..10813)
                     /locus_tag="ACHA6Y_001602"
     CDS             complement(8036..10813)
                     /locus_tag="ACHA6Y_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8987699.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            11004..11228
                     /locus_tag="ACHA6Y_001603"
     CDS             11004..11228
                     /locus_tag="ACHA6Y_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987700.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3329 a         2442 c         1890 g         3904 t
ORIGIN      
        1 aaaaaagatt cttttatata agtgaaaaaa gcttttttcc accaattaaa taaatctctc
       61 atctcaatag tttatccatt attttatctt tttttgcgta tacctactaa atattaccgt
      121 cttcttctcg ttcttctact ttgttcgaga gaatctgtac tagaaaaagt agttgtatgt
      181 ctaccttttc tgcctatccg agactttctt ttcatatatg ggatataaat gattagatac
      241 agtacagcaa aaaataaagc aaatacgcaa gcttcccaac tacctaacgc caattcaacc
      301 atcaaggtag caaccccaac ggatgctgca taccagataa aaatcagaaa cgtttctttt
      361 tttctgacca tgcgaaacat gataaataga ctaataagca atagtggcaa cacaaatatc
      421 caattgcttc cttccatcag ccacttgagt atatcaagca gcgactgtgc aacagcgact
      481 aattttccca tagtgaacac acaccttttt ttataattaa aaaattcgcc tttttattct
      541 actatgaatc ttctggcttg gctttcattt ctcttatgta atcattttct tttatacaac
      601 ttaaaaaaaa attaaaccat tagcataata ttttctgaat ctattcacga aagtcttttt
      661 ctatctttcg ttttccacga ctcaactgat ctagagtcaa aaaactaaaa cgacagttgc
      721 gcaaatgacg cacaagctgc cgctttaata acaaatagcc tttcattctc agcaggccac
      781 ttatttccag ttaactttct gttcttagct ttttactcaa ctggataata tatgcactta
      841 atgaagtccc aatttccgaa tgttgaagtc catattcaat atttgtcttc atatatccta
      901 acttatcgcc tacgtcgtat ctttttcctt taaactctct cgcatagacc gcctctgtct
      961 ggttaagagt ctgaagtgca tccgttagct gtatctcgtt tcctgctcct ggttcttgag
     1021 tttctaatat ctcaaatatt ttaggagtca gtagataacg tccgataatg gctaaattac
     1081 ttggtgcttt tcccatttca ggtttttcta caaacccttt tactctgcaa cgaccctttt
     1141 cgctatctgt tttaggatca ataattccgt atttagatgt ttcattttct ggtacacgca
     1201 tgacggcaac agcggaagct tgtgtttctt cataatccat catcaattgt tgagtcaatg
     1261 ggatatcatc tttcatcaaa tcatccccca gcatgacgac aaacggttca tttcctataa
     1321 atgcttttgc ttgaagcacg gcatccccca gtcctcttgg atgagattga cggatgaagt
     1381 gaagattgac ttgagtcgtt tcttcaacta aagcgagtaa ttctgttttt tccttttccc
     1441 gcaaattgtt ttccaattcg atatttgcat caaagtgatc ttcaatgggt cttttttctt
     1501 ttcccgttac gatcaagatg tcttcgattc ccgaatccaa tgcttcttcc acaataaact
     1561 ggatcgttgg tttatccacg attggaagca tttcttttgc catggctttt gttgccggca
     1621 aaaaacgtgt tcctagccca gcggcaggaa tgactgcttt tttgaccttc acttgctgtt
     1681 cctcctaaac gacgattaac tattgatcat ctatttaacg aagagaaaat tcattctcca
     1741 tttttccgtc acgtaacatg atttcttttg ctgctttttt gatatcttct ccattataca
     1801 aaacattata gattgtttga gtaattggca tctcaacacc tagctgttga gctagctcta
     1861 ctgctgcttt tgtcgttgcc acaccctcaa cgaccattcc catgttttct agaacttctt
     1921 ccagcttatg tccctgtcct aacaagttac cggcacgcca attgcgagaa tgaacacttg
     1981 tacaagtaac gatcaagtcc cccacaccac tcaaaccgat aaatgtcaga ggattggcac
     2041 ccatcgcgac accaagacgg ctgatttctg ccaatcctcg agtcatgatt gctgcttttg
     2101 catcatctcc gaatcctaat ccatggatag cgccggcacc aattgcaatg atatttttca
     2161 gtgctgcacc agtttctacg ccaatcacat cattatttgt ataaatcctg aaataatcat
     2221 tcatgaacag tttttgtaca taggctgctg ctttttgatc aatacttgca gctgtaattg
     2281 ttgtgatatc atgtactgcc acttcctcag catggctagg accagataat acaacaattg
     2341 cctgtctttt ttcttctgga atttcttcag ccaaaacttc tgagattcgt ttatgcgtat
     2401 cttgttctag tcctttactt gcatgtataa tgattggctt agtattcatt ttaccaataa
     2461 gttcctgtgc gactgaacga atcgcttttg ttggaacaac aaataaaata gcatctgcgt
     2521 tatccactgc ttctgccaat tctttatatc cgataatcga ttctgggatc gtcaaatcag
     2581 gtaaaaaatg ctgatttgta tgatacttat tgatttcatc aatctgttca ggtacatttc
     2641 cccaaatacg gacatcatgc ccgttttctg ccaatgtttg tgctaaagca gtgccccaag
     2701 atccaggacc aagaactgcg attttttgtt tcatggatat tcctccaaat aaataattga
     2761 taatttccat tatagtatac atttcttaaa gttgggacta atttccattg tttttaagtg
     2821 tttgtttact tcttttatcg tttttacttg aaataatcaa tgatatgtta caatagtcta
     2881 cattttattt ttacactcgt tatgctttgc ttgttcctaa gaggaagcag ttttgacaat
     2941 gttactacca gcattactat taggaggtta ggaaacttct cctagtgagt attgatttaa
     3001 aatacataag agacagattg aacatttcta aatctgtcag gtcctgcaac ggacagattg
     3061 tctctgttga cggttgacat aggaactttt gttttagtca acattctgga tacaaaacgc
     3121 tatgtaaact ggcgcaactc gttgtacttg gacttttact acggatccat gcaacaacta
     3181 aatagtacta ttgcataaca aaaaatggtt ggcacccaca tgtcccatta aacagattat
     3241 aagttgaact gtagataatg caaaaaatgt gttttataac gtaattgatg ccaatatttt
     3301 aaaaaaataa agggagcagt tatcgctgct ctctttattt tttatattct ttttatccag
     3361 tctatcctaa taatcaaact gacgatagta gcggcgtatc tctaatggat ggcagttcaa
     3421 ctgctccaca tgagcatcga tgcgttgtgt cactacatgc atcaataaga aagacaaaac
     3481 acgtccgcga tatttttcac tgataccttc tactggatag tcttttgtgt ctatcaatgt
     3541 ataattgctg caaatttttg gcaaaagttt agccacacgt tcagataaca ctctctgttc
     3601 atcttcgcct aaaaataatg tgactggggt tgtttcttct acgatctcta acgtcccgtg
     3661 aagaaactcg gcagcatgga tagatttcga tggaagccag ctttgttctt cccagtaaca
     3721 catggcatat gaataaacag ctccccattg attccctgct ccaataaaat aatgcatact
     3781 atcacgtcga tgtttttctg caaatgataa gccaaatgca tctgcttttt tctctgcttc
     3841 cactaaccct gttggtaaat aacgttcgag ctgttcatag tattctgaat aagcttcgaa
     3901 ttctccattc aaatacatca atcgatctgc tgccataaaa aatttgattt gttctgttcc
     3961 aggagctgga taagtgacca caaaatcgca tagctgagac aacttactgt tcgctttatc
     4021 gataaatcca atcagtgtcg ctcctacttt cttgatttgt tctactgctt tcactacttc
     4081 ttgagttgta ccggtaacag aggacaaaat aacgatagaa tcttttgtta atcttttgtt
     4141 tccagtagtg taatattctg cagcatgctg tacaaagaca ggcaagttgc ttttcccatt
     4201 catataagtg acagcttgca tcgaggaagc atacgttcca ccgattccta gataatagac
     4261 agcgtcaaat ttttccgcat aaagtttatc gatgatttct tctacttgcg gacgtaaagc
     4321 tagtgcgcct tttattgctt tgatttgttc ttcttcattg aatttcagca tgatttccac
     4381 ctcttataat ttacttttca agactgtatc aaagtcttca aaaatctttt tgttcaagtc
     4441 aataccttta gctactgcgg ttttataagc gaatagttga aacggaacga caagcgctaa
     4501 tacagacaga tattcatcac attctattgc taatccaagt gttttctcgt ttcttgcttt
     4561 ttttgtggta atggtgagag tctctccaac gtaccgactc atatatcttt gcaacttttg
     4621 cgaacgttcc ttatttacac tttccccttc aataaagaat agcaaatgcg aagcatctgc
     4681 ctccaggtat gggccgtgca tataagcttc taactcaaat ccttgtgacg gaacgcggat
     4741 tgtttcagtc aatttcgtct ctgcttcttt agctgttccc cagtttggac catatccaat
     4801 ggtgacgaaa cgctgtgcta aacgaaataa actttgatat ccatcaaaaa attcttccgt
     4861 tttttgaata atcgctggta aatggttgat tattttgcgc aactgttgca tgtagtcttg
     4921 ttctattttt ttgctgattt tatttttact tattccaatt cctatcccta agagtagtaa
     4981 ctgtaaaaca gtcgcagaat agccttttgt aacgaagcct actgattcta ccccggcacc
     5041 taaatcaata atttgtgcag cttctaaagc aagtggactt tgcacgtcat ttgtgatagc
     5101 aacaacaggt aacccttgtt tttgtatcat ttttactgct tcaacagtag aagcactttt
     5161 cccgctttgt gacacagcaa tcacagtatc gattgaagga gaaagttttc cataattgaa
     5221 aaaataaaaa ggttcttgaa tatcgacagt aagatcacca taagattcca atgctggctt
     5281 cgctgctaaa caagcgttat aagatgaacc cgttgctaag atcagtaaat tcactgtttt
     5341 tttcatatgt tctcttgtcg aaaaatcatt ctttttcaag atcgtactta gtacttcctt
     5401 ttcttcatag atataatctt gcatcgtaaa cattttctct gctccttttt taaaaaatcc
     5461 ctatccacga accaaaaatt ccaaagaaag ctagtccgat caagatcgtt aatggtttca
     5521 cttctttctt caatagataa aacactatgc caaatgctcc aagacataag atatttggaa
     5581 cgatatcatc aaaaatactt tgaacagtca cagcattttc tcctgaaccg atttttaaag
     5641 gaatattgat ggtgatcatc gtggcagtca ttgctccaac gaccatcagt ccaataatag
     5701 acgcgcctaa tgtcaaactt tccatcatac cgtttgcctg gatctttttc aaaaaacccg
     5761 ttcctaagcg atatccccaa cctgttgcta gatagcgaaa cagaatgtgc ggaatattga
     5821 aaatcaaaat gaacaagata gggcctaaaa tatttccttg catggctaaa gaggtaccca
     5881 cacctgtggc aatcaatcgc aaagttcccc aaaagaaaga atctcctaac cctgcaagag
     5941 gtcccatcaa agatgttttg atactatcga tcgcagtcac atcaaattca ggatcatcct
     6001 tattttcttc ctccattgca gccgtgattc ctaaaatcag tgtaacgata tgtggggtcg
     6061 tgttaaaaaa ctctagatgc cgtttcaaag ctgctgccat ttcttctttc gactgatata
     6121 actttttcaa aatcggaatc attgcataaa cataagccat attcatctga cgttcataat
     6181 tccaagaaaa ctccatttga aaagaacgcc aaaatacgtg atttagctct tttttcgtta
     6241 ttttgctgtt tttgatctca gaattcttca tcttcttcta cctcccctgc agttgtttga
     6301 agtggtagtt gacggacatt cataatattg actaccacaa ctgctaaaat cgcaccaaaa
     6361 atagctactc cggtcacagg aatctctaag taagaagcta tagcaaaacc taagaaaaaa
     6421 tatggtgcaa cttgtttgtt gatcagcaaa cgagcaagca tcgcaaaacc aagagctgga
     6481 atcaaccctg tagctactga caaccccttt tgaacgaagt caggaatcat atttaataga
     6541 tttccgatcg tattgcttcc caccatataa gaaaccatta ccacaacacc aagcatcaag
     6601 gaaagaccga taccggcaag caagtgcata tgctcgactc ctttataatt tccttcttct
     6661 gcataattgt ctgctttatg gttcataatt ggaatcaaaa gccctaagta aatatttttc
     6721 aatatcaatg tcaacgtagc aatcggcaaa cctagtaaaa gcgcggtttc tgtaccggct
     6781 ccagctgtaa tagcaaaagc cgtccctaaa atcccaccgg tcacaacatc aggtggaatc
     6841 gaagcaccta ctgagaacga tccgataaat gctaactcta aagttgcgcc cataatgatc
     6901 ccagttttta catcacctaa cacgattcct gtaaacagcc ccgtcacaat cggacgagaa
     6961 agcaacgatg tccctagtgc atattctgat tgtgcgacaa atgcaacaat tcctaataag
     7021 actgcttgta caatcattga ggttcttcct ttcttacaaa acttctctta catccacttt
     7081 tttatctgct ggtacttgac ggatttctac ttctatttgt ttgtcaatta atcgtttcaa
     7141 gcgttcctcc tcttctggta aaatatttac cgcttttccg atgctgcgag taccaggttt
     7201 tgccttgatc cctcctaagt tgatggtttt gatttctgga tacgcttctg ccagcttata
     7261 tgcatcctcg atcgattcca ctacgatgaa taacttatat ttatctgtca ccccgctttt
     7321 caacgcggca atcgagtcat caattgattt gatcactagt ttgactcctt gtggcttggc
     7381 taatttgatc gttgtctttc gtatctcatt cgtaggtaca tcatcattag caatcaaaat
     7441 acagtctgcg cctattgttt gtgtccatga aaaagctact tgtccgtgta ataaccgatg
     7501 gtcgactctt gtcaatacaa tcatttttct tcctcctaaa attcgtcttc ttctattttc
     7561 tgattgagcc gctcgttaca aaactggatc attttttttg aatcagttag cacctgctgg
     7621 atcaaaacag ttgtctctac ttcagcgtcc aaccgagata ctagctcaat caatagtggc
     7681 aaattcaatc cggcaacgag atagacatga tcccgattga tatattgtaa gaattcgttg
     7741 ttgacacttc cgccaaaaag atccgtcaga acaatgattt ctttatcttt gtacctcata
     7801 aaaagctgcc ttactgtttc cgctaaatca aaattctcat cagtatagca atcgaataca
     7861 gtaatagaat gatttttccc atagatcagc tctaatgaat ctaatatccc tgaagcaaat
     7921 tttccatggg aagctaagat aatttctcta tccacttttt catctcctct tttcacttac
     7981 acatactaaa aaagcaagtt gcatgccaaa attagtggca acaacttgct tatgttcaaa
     8041 attcttcttc tatcgagaga aactctgtat cttcactcaa aatatcatag atataggcaa
     8101 tctcagaatt ggggatcatg acactataat gctcttcaat gacactaaac gcagatttaa
     8161 tagcagataa cccttctttt tgacactgct tccatttctc taatccttga taggtttcga
     8221 ttggcatttg tcggatcaat cgttcaatga gacagctcac atggacatac aacgctaatt
     8281 ttttgggatt gctgatgctt cgattcaact gcacctctaa ttctctcata aaatgatcga
     8341 tttctcccat caccttctct gtatctagaa tagtcacgga atccagtact ttttccaatg
     8401 aaaaattacg aatgatgcgc tgattgaata tttccttctc tgaagcattc atagtagtgt
     8461 ccaaccattc agctagtaca gacatctttt cccccgagat gaggtcttct aatgaaagat
     8521 agtcgatatt ggtgatatca ggatctgtgg tgccaatcat tcctacgact tcgtatatgg
     8581 aaaataatgg gtcactgttt ttcttatctt ttaattgctg atattcatat gggatgattt
     8641 tcaactgaca agccgtaggt aagctttttt ctagcagttc acttaagtgg accgccgtac
     8701 cgattccagt gaagcaagta gtaatgagtg ctttgggttt gttttcctct ggatagagca
     8761 gtcgccattc tggctggatc tgtcgaacag tctcttctgc tatctcttct aatgatacat
     8821 ttttttgcag gcattcccct actgtgattg ctaaaggtgt cgtcacatta ttcaaaatca
     8881 aaagcggtac ggataactgt tttttaaaaa actgatggat ttctttcaga gatcccatgt
     8941 ctactaatat gatcaatcca tttgaaacat cgctgttttc gcaataatca aggatttctg
     9001 aagcaatctg ctgtggtgtg acatctagtg gcatatcaaa tgattcaaat agatcctctt
     9061 gtaataaacg atttactaca tttgccatac tgctagcagt tgcataaccg tgagcagcaa
     9121 tcatcgcttt tggcagtcgt ttttcttttg accatttcgc tttttttaga tagatcgtca
     9181 gtaaaatccg atccatactt gtgtcttcaa tatctaattt gatcttactt aattccagaa
     9241 ttcgttggac atagtgataa ctagatggat aagactgaga aacttgttgt tctaattctt
     9301 tgattagttg gataatatca tgttcttccg gtaaccattt gatagtgctt cgctgataga
     9361 gatagtaact caaggcatag acactgtttc catcgaattt gatttgatag gcagtttcca
     9421 tttgtcgaaa ggtctctcgg atagattgtg tccaataaac cagcagctcg tgtttttctt
     9481 gtctactggt ctcaaacagc aaaaaatcaa ataattgaat cacttcttgc tttaattttc
     9541 cttcgcaaga atatagcaga cagttatttt tcttgaaatc aattaggatt tgctcaaaac
     9601 ttttaacgat tcgctgctgt tcgggctgtt gcttctccat taattgctct aaggtctggt
     9661 cttgcgtaat atagattcct tgctgtgctg tatttttagt aataggctgc gcaagtagtt
     9721 ctttttgcaa atgatggatc gtgacatgga tttcaggctg ttcacgctgt tcagcatatg
     9781 cttttgctgc tgaaactttg acgatatttt tcagctctcc tatgtttccg gataatttct
     9841 gacttgttaa aagtgacaaa gctggtccac tgactgacaa agggctcccc atttttcttt
     9901 gttcttcgag aaaaaaagag taaatcagtt ctagccgttc attacgacta cgctgattaa
     9961 gtgcaggtag ttcgattcgg ataggtattc ttcgaatgaa agttgttaag aaatgactag
    10021 taatctcttc agtcgtagca aaaaagagac gtgttttgat ccggcgtgac aaattaggtt
    10081 ctcccatacg ataaatcaca ccttgatcaa gataggtgaa cagtttttcc tgaccttctg
    10141 gtgttaagcg atgtacctcg tctaaaaaca agatcccacc atctgctgct tcaaaagccc
    10201 cttttttatt ttcttctgca ccagtgtacg ctcccttaac atgtccaaac aaattgctag
    10261 tcaaaagttc aggattatct gcatattgtg cacaattaaa agtgatgaat ggtgcagaat
    10321 cttctagcag atcatgaagc agacaatact gatagaccaa attaactaaa taacttttac
    10381 ctgtgccact ttcgcctgta atcaaaagtg gtaatccgcc atctggatag taaagagctg
    10441 ttttgatttg ttcgatactt tctttcaaac tggcgtcgta tccgatcata aaagagaata
    10501 agtcttttgg ttgcgcgaaa aaaggctgtt cctctttgag atcatcgata gaaaaataaa
    10561 aattcgtgcg taacttaaaa aattgttttt caaaagcttc tttgtggaaa taaatcactg
    10621 gtcgagaatt gattttgact agtagttgct catcatttaa ctggttcaaa taatgactga
    10681 ctgtgtttct ttttacccca aaatgatcag ctaacctttg tgctgtaaag acctcgttca
    10741 tatgttctaa atcgaaaaag gctgtctggt tctctaagta acataaaatt tccttcttca
    10801 acaacttatt cacccctcct ccatcataga aaacggattc attttgtcaa taaaatcttt
    10861 aataaagaat tgaaaaatat atcttattat agacagcagc ctacttatcc cttataattt
    10921 ttattctaaa aaacgtacag ttccttccag tttttatact ggaatttcaa catatgaaag
    10981 aataaggagg ctatgactat tcaatgaaat cggataaata taaaaacagg aaacatactt
    11041 cctggaaaac ttttcgagaa atcatcggat cttatctcgc tgtagttgta tttttgcctt
    11101 tgctttatat ggctatgaca gaacaaactc gttttattgg cgtagatacg caatatatag
    11161 ccgaaacagg atttttattc atctttatct tgatttttgc gttctataaa tccttccata
    11221 caaaataaga ttcacagatt tcaactttcc tgtctgtttt tagctattac aaatccttat
    11281 atgtggtata acaacaattt attggcataa tgaggaaaaa cgagttatga tgctttcaga
    11341 gagtaaagag gtactgttgt caccatcctt tttattgatt acgtacacca ctcttagttt
    11401 tttcccattg attacgaaaa caaggtagaa acattgatgt tcctaccttg ttttcttttt
    11461 ttattttcag atcaaaatct caagtggctt ttgacttgat gtcgggaggt tgtcttcgtt
    11521 ccatcaaacc tgatagaatc tgcgccaaac catagttttc tccac
//
LOCUS       DAYYHX010000082        11524 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000082 DAYYHX010000000
VERSION     DAYYHX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.083"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(121..726)
                     /locus_tag="ACHA6Y_001604"
     CDS             complement(121..726)
                     /locus_tag="ACHA6Y_001604"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8987701.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            922..1845
                     /gene="coaA"
                     /locus_tag="ACHA6Y_001605"
     CDS             922..1845
                     /gene="coaA"
                     /locus_tag="ACHA6Y_001605"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM8987702.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            1904..3469
                     /gene="guaA"
                     /locus_tag="ACHA6Y_001606"
     CDS             1904..3469
                     /gene="guaA"
                     /locus_tag="ACHA6Y_001606"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8987703.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(3960..5591)
                     /locus_tag="ACHA6Y_001607"
     CDS             complement(3960..5591)
                     /locus_tag="ACHA6Y_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM8987704.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(5605..7278)
                     /locus_tag="ACHA6Y_001608"
     CDS             complement(5605..7278)
                     /locus_tag="ACHA6Y_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8987705.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(7291..7608)
                     /locus_tag="ACHA6Y_001609"
     CDS             complement(7291..7608)
                     /locus_tag="ACHA6Y_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8987706.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(7633..9033)
                     /gene="lacG"
                     /locus_tag="ACHA6Y_001610"
     CDS             complement(7633..9033)
                     /gene="lacG"
                     /locus_tag="ACHA6Y_001610"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8987707.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9270..10055
                     /locus_tag="ACHA6Y_001611"
     CDS             9270..10055
                     /locus_tag="ACHA6Y_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8987708.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            10058..10750
                     /locus_tag="ACHA6Y_001612"
     CDS             10058..10750
                     /locus_tag="ACHA6Y_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8987709.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(11024..11395)
                     /locus_tag="ACHA6Y_001613"
     CDS             complement(11024..11395)
                     /locus_tag="ACHA6Y_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM8987710.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         3491 a         2476 c         2105 g         3452 t
ORIGIN      
        1 ctttttgaag aaaaaatagt tctttgtttt taaaaacttc tcaacaaaaa aagaactgat
       61 acgtacccgt acatgcacgc ccagttccac tctttcgtcg ctccactatt tttcagctag
      121 ttattctttc acacttttga tgatataata tcctttatct tttgtgacga tttctgcatt
      181 accaaatgtt tcttccattt tcttttgcgc actcggcgct ccttgtttct tctggatcac
      241 aattgtcagt gtaccgccct tcttcaaccg aggatatgcg cctgtcaaaa tttcatgtac
      301 aacttttttt ccagcacgga tcggcggatt gcttacgata gctgcatagg tttcttcatg
      361 aactgcttca tagatattcg attgatggat atcaacatgt tcgatttggt tacgctttgc
      421 gtttccttgc gctagactta ccgctcgttc attaatgtcg accatttcaa caaaccgctg
      481 tgtcgcaaat gccaaagcta acccaatcgg tccatacccg catcccacat ctaagatctt
      541 tccttcatca ggtaactctt cccaatcgaa agcatcaatc aggacacgtg aaccataatc
      601 cactgtttca cgtgaaaata caccagaatc tgttacaaat tgaaaggttc tgccttttaa
      661 ctcaaaagcc cattgttcaa aatcgtgcgc cgtatctggg cgttcagaat agtaatgatt
      721 tgtcatgtct tccatccttc tcatttcttt catgacaaca gtctatcctt ttatgtatcc
      781 gtgcgcaagt caagaataag actccgtttc tttctattat aaagattata caaaaagatc
      841 ggcaatctct cttaagtggt ggggtttgcg tttattttgt gttaaaatag ttccattgat
      901 gggagaacgc aggaggctaa tatgaatgat ccaatgaact actaccgtgt ggcaagagaa
      961 gaatggcgag aattttatcg taatgggcaa gcacctttga cacaagatga actagataat
     1021 atcaaaagtt tgaatgaccg catttccatg caggatgttc gagatatcta tgtacctctt
     1081 gctcatctga tttatcttta tatgaaagaa tttgaatctt tgttcttgag taaaggttta
     1141 tttcttcata aatacgtaga agtaccgcct tttattatcg ggatcgccgg aagtgtagcc
     1201 gttggaaaaa gtacaacagc cagactgctg cagacgattt tgtctcgtac cttcaaacga
     1261 agaaacgttc agctgatcac gactgacgga tttctttatc caaatcgtat attaaaagag
     1321 cgtggaatca tggaccgtaa aggatttcct gaaagttatg atatggagat gctgatcgat
     1381 ttcttgaacc gtgtaaaatc tggtcaggaa gagataaaag tacctgttta ctcacatgat
     1441 gtgtatgata tcattccaga caagtacgaa ttgatccagc agcctgatat cttgatcgtt
     1501 gaggggatca atacgcttca attacctgcg aatcagcaaa tctacgtcag cgactttttt
     1561 gatttttctg tctatgtcga tgccgatccc gtgctgatcg aaaaatggta tttggatcgt
     1621 tttggtgcgt tactggatac ggctttccaa aatccagaga attattatta tcgttttgct
     1681 atcggagatc gtgaagaagc ctttaaaatg gcaaaagatg tatggaaaaa tgtgaatttg
     1741 aaaaatttac aggaatatat cttgcctaca cgaggaagag ccgatataat tctccataaa
     1801 actgaaaatc atatcatcga ttcgattttc atgcgaaaat attagcattt taaaatagtt
     1861 tgactatttt gagataaaat ttaaagggat aggggtaata aatttgacaa acgttgccga
     1921 tatgacgaac gttgaaaaga ttatcgtatt agactacggt agtcagtata accaattgat
     1981 cacaagacgt attcgtgatc ttggagtctt ttccgaatta ttgagccacc gcattactgc
     2041 agaagaagta aaagcgatga atccaaaggg aatcattctt tctggcggac caaacagtgt
     2101 atatgatgag aatgcatttg ggatcgatcc tgagatcttc aatttaggta ttcctgtatt
     2161 agggatttgc tatggtatgc aattgatcac gtacaaactt ggcggttccg tagagcctgc
     2221 taaaaaccgc gaatatggga aagccttgtt agaagtaaca agcgatcaag ctgtcttgtt
     2281 taaagaaaca ccaaaacaac aaacagtatg gatgagccat ggtgacttgg taactaaagt
     2341 tcctgaaggc tttgaaacag tcggaacaag caaagattgt ccgattgctt ctatccaaga
     2401 cacaacacgt aatttttatg gtatccaatt ccatgcagaa gtacgtcatt ccgaatacgg
     2461 aatcgaattg ctgcgccact ttgcctttga tgtttgtcac tgtgaaggta actggacaat
     2521 ggataacttt atcgatatgc aagtagaaaa aatccgtgaa caagttggtg acaaaaaagt
     2581 cttgcttggt ctttcaggtg gcgttgattc tagtgtcgtt ggggttcttt tacaaaaagc
     2641 gatcggtgac caattgacat ctatctttgt tgaccacggt ctgctacgca aaggcgaagc
     2701 ggaacaagta atggaaagtc taggcggaaa attcggcttg aacatcatca aagtcaatgc
     2761 aagagatcgc ttcttagaca agttgaaagg tgtatctgac ccagaacaaa aacgtaaaat
     2821 cattgggaat gaattcgtat atgtttttga tgacgaagca acaaaacttg ctggtgaaga
     2881 aggcgtctct ttcctagcac aaggaacgtt gtatactgac gttatcgaat ctggtacaga
     2941 aacagcacag acgatcaaat ctcaccataa tgtaggtgga ctgccagaag atatgcaatt
     3001 tgaattgatc gaaccattaa acacattatt caaagacgaa gtccgtgaat tagggactca
     3061 gttaggcatg cctgattcaa tcgtttggcg tcagccattc cctggtcctg gtcttggtat
     3121 tcgtgttctt ggcgaaatca ctgaagacaa gctagaaatc gtacgcgaat ctgatgctat
     3181 cttgcgtgaa gaaatcgcag ctgctggact tgaccgtgat atctggcaat atttcactgt
     3241 tcttccaggg atccgctctg tcggtgttat gggtgacgga cgtacttacg actacacagt
     3301 cggtatccgt gctgtaactt caatcgacgg aatgaccgct gactttgccc gtatcccatg
     3361 ggaagtcttg caaaaaatct ctgtacgtat cgtaaacgaa gtagcccacg tcaaccgtat
     3421 cgtgtatgac atcacttcta aaccaccagc aacagtggag tgggaataaa gagccaacat
     3481 aatatggagg gggaatccct ataaatcaac attcttcaat agctaaaatg cttgattttc
     3541 attaaaaata agatggttcc ctaccaaaag agactataat ataagttaat ttgtttcatt
     3601 cttatcataa aaagaagttc tcgaaaaacg aactgttttc cgagaacttt tttctatcca
     3661 tctaagtgct caaatcatat tcacgacgga attctaaggt taccctttct taaacaaact
     3721 ttatgttatc tgcacaactt tttgcaattt tttcattgct tcttacgaaa tcgttgaact
     3781 ctctttagca taatacaaag tgtagatgaa gtctggtgct ttactcgttt tggtaaaaat
     3841 gactggaaat cgtttctggt tatttttggc aaaggcgagg tgcaggttcg attcctgcct
     3901 tctacgctaa cgatgtggct gaagggaacc tccgatcttg aatcgaaacg atccgcagtc
     3961 tattgtttca ccaatgaaac aaaatcaacg ttcgtccaag catttcctga agccttgcta
     4021 taaaccccaa tttcacaaac acctgttgta attggaatgt ctttgattgt aatttttgtc
     4081 cattcacttg ttttaacaac cgcagtattt agttccgctc caccataatt tttgatatat
     4141 aactgattag tatcaaactc tcccccagac attacatagg cactcaatgt gtaagttcct
     4201 ttgcttatgc cactaacatt ttgataagct gatgcctcgt atgcttgatc ctgccaaaaa
     4261 gttagtttgt agtccccaaa aaaagtgccc gcttccgttt tgatcgtacc cttagcggtt
     4321 ccttgttgaa accaggtttg ccaattggaa ggactggtcg ttggtccatc gctttcgaaa
     4381 cttgcatttt taactagatt tatttgcgcc cctgcttgtt taaacgcacg caagcttggc
     4441 aaggcgtttc cagcaaaatc aaacatcgct tggttgtccc attcgtttcc catagtggat
     4501 tgaacactga gataattcat acctgcttgt gttgcccaac tcacatttcc gttgtaccaa
     4561 agaggttccc aatagaagaa gcccgccccg cgattattcg gtactgcctg aatccgatcg
     4621 gcaagatcca gcaaatactc tgcttgtcct gtcggtgttg gttcatatcc ccccgcagct
     4681 gcttcggctt caccaaaagc atttggcata gtatcggcat tcgctgttgt atgcccataa
     4741 gctgtttcaa ctacgatcac ttctttacca taccgttggc tgatcgcatt catattggtt
     4801 tgaagttcat tcattgaacc gtgccaatac ggataatacg acactccaat taaatcaaat
     4861 gacacacctc gactcgtgat ttcatcaaac caccaagtaa atgctcctgt atcgccacca
     4921 tcagctaagt ggagcattac tggagttgtt ttaggttgag tgtctcgaac agcttggata
     4981 ccagacttca aaaaagcagc caatcgatca aattctcccc cgccttctcc ccaagacttg
     5041 ccataaggcc agagcattcc gctattcagt tcattgccga tctgaaccat atcaggataa
     5101 acgtccagct gcttcatttc cgacagaacg ttctttgtat agtcgtacaa tgccgtattc
     5161 atttcttcaa aggtcagacc ttgccatgct ttaggaagat tctgtttccc aggatcaacc
     5221 caaaaatcac tgtagtgaaa atccagcagc accttaagtc ccagattttt tgctcgtttt
     5281 gcaaggtcaa tcgtcgtttc cagatcattt gtccctgctc cataactttc tccattacta
     5341 ttataaggat cctgccacaa acgcagtcga acatagttga caccattatt ttttaaaatc
     5401 tctaaggcat cttttgaaat atcgtcttca taatattttg caccagattt ttccatatct
     5461 gacaggatcg aaatatctgc tccgcgaata aattcgctgg cagatgcttc aacagttttt
     5521 gaaacagccc ccaaagcccc taacacagct gcaaataaaa cagctttacg caaccaaatt
     5581 cccattttca ttatggctcc tcctttattg aaattggttc agaataaact gaacagcttt
     5641 ttcaggatct ctcgttaatc cgatatattc tgctcctttt gtcgcaacca gcttgattcc
     5701 taagcgatcg gtgtcttctt tgatatcatc atagtaagaa ttgacttgcg gtgctaagac
     5761 caccatatca aaatcctgca gcatttcgta atgagaacca taagcacccg ccattgcact
     5821 gatcggtgct ttcgattcag atgcaccttt ttttaaagca ttagctaaca tcgcacttgt
     5881 ccctgcgcca gcacaaagta ccaacacatt gatttcttta tttgcatcgc tgataacttg
     5941 actcgctgcc acaggtactt cctcacgtac atcggcagaa gcaccttcct cggttgcgat
     6001 cacctctgct ttttgtgctt ctttctctac taagacagca tcgtatgctt tgcaaaatgg
     6061 tagatagatc aggaagtcaa cgacgattag aaccgctgct agaacaaaag ctaagatacc
     6121 catgcccgtt cctagaacca aaccgatcgg tgctggagtt gcccaaggca aaacgtacat
     6181 aaagctgttc atgcccaata catcgacaaa aaacttgaac aggcaaacgt ttacaacagg
     6241 tgttaacaaa aatggaataa aaaaataagg gttcaaaatc atgggtgccg caaatagcaa
     6301 tggttcgttg acagcgaaac ttactggaat aaaggaagct ttcccaactg ccttcaactg
     6361 cttagactta gcaaagagta ggaagataaa aggaacaacc aatgttgccc cagttcctcc
     6421 catagttcct acaaaattcc ctaaacccac cgtcagtact ttagaagctt gttcaccgga
     6481 attgaacaat tgcagattgg tttccacatt ggcatagata attgccgcaa ttgccggctc
     6541 cacgatcgac ggtccatgaa caccgacaaa ccaaaacagc gccatcgctc cccagataat
     6601 agcgattcct aagtacccgt cagctgctgt aaatagcggc tgcagcaccg taatgattgc
     6661 ttctgcaaat gagatttgga agatttgtcg aatgcctaaa tcaattaaaa cggccgcaaa
     6721 tactgaaaaa gagaaaggaa aaatatctcg gaaggtttgc gaaatcgttc caggaacttc
     6781 tttaggcatt ttaatggtaa tatctttcag cacacaaaac ttatataaat taactgttaa
     6841 aaatgcagcg acaaaggaag ccagtagtcc ctttgtcccc atatattccg ttgaaaatcc
     6901 accttcaatc ggagagacgc ttaacaacag gaaacttacc acagaggcca gcatcacaga
     6961 ggtggtgttg attactttgt ttttcggtaa gcgacgattc atcgaatcag ccaagcagcg
     7021 agcagtggtt gctgaaacga gtaaccctac aacacccatc gaataattat agactttcca
     7081 tagccatgtg gagacagatt caggccattg gtaaccaaaa atctcaggaa ccgcagcaat
     7141 caaaatgaaa atgcttgaaa ataaaatagc tggcatcgct gttaaaaatc cgtcacgaac
     7201 ggctcctaaa taactattac gtgagatttt ttcaaaaaat ggtttccctt tttcgatttg
     7261 tttaatgatt ccatccatag cgttcttctc ctatccttga ttctgtctgt acagtacaat
     7321 aaagtcttga atcagatctc tcagcaataa agtggtcatt aaatgatcct gcccatggat
     7381 aaaaatgaat cccatctcca tctctttccc agatgcttct tccgctaaaa tttttgtttg
     7441 cgaattatgt gcaagggtta aattttcgtc ggcttctttc aacgccgaat caacatgttc
     7501 aaagtttcct tgtcttactt ctttcaataa tttcaataat gtactgcgag cgtcgcctga
     7561 atatgcaacg atctcaaaac caagcatttg taattcctct ttattcatct tttcttcctc
     7621 cattatcact tattaaataa tcgttttggt ttcactgacc aacttgtacc agtaagctga
     7681 ttcttttggg taacgcttct gggtttcaaa atcaacataa aacaagccat accttttgtt
     7741 atacccattt gtccatgaga aaagatccat aagcgaccac aaaaaatacc ccttcacatt
     7801 taccccggct gttatcgcct tgcttaagga ttccaaatag actctcaaat agtcgattct
     7861 cggctgatcc ataataatgc catcttcaaa ctgatcttta taccccatcc cgttctcagt
     7921 gatatagatt ttgttgtaat gtgggtaatc gcttttaatt cgcagcaaca agtcatatag
     7981 accttctgga taaatcagcc agtcccaatc agttcgagga attccttctt tataaatacg
     8041 ttctccgatt cccttcactt tataaacaga ggtccctttt tcacccgtac cgttatgatg
     8101 gattgcattt tctccatcgt aagctttgac aaaatggcat tggtagtgat tgatccctaa
     8161 gtagtcatta cgcgtggatg cttttttcaa ttcaacgaaa tcttcttcag gaaaatgata
     8221 gctggcctga tttgcttcac atatctcatc aagagcagtc aaggtttcag ttgaataata
     8281 gcctaaatag gtagcatcta ataagaaacg gatcgacaat gcatcgtcta aaaaggcagc
     8341 atgcttatct tccggcgcgt ctgttgccgc atatttcgtc tctaatgagt gaactacacc
     8401 aatttcaccc ggaagttcgt tttctttgaa atagttcact acacgggcat gagcaaccat
     8461 catattatga agacaggcga cgatctttgt aaaatcatat ttgattcctg gtgggaaaac
     8521 acctaacaaa tattgattgg ttgcaactgg atagatttca ttaaaggtac tccacacctt
     8581 gacttctttg aattcatgaa aacaaaaaat ggcataggaa acaaatgctt ctattgtttc
     8641 tcgattcaaa aaatctccat ggtcaaacaa acgtttaggg gtatcaaaat gatgcaacgt
     8701 cacaaacggc gttacgtttc ttttcttaca ttcttcgaac acacgatgat aaaaagcgat
     8761 tccttctgga tttggtttcc cagctccatc aggaaaaatg cggctccaag caatggataa
     8821 tcttagacca tttacaccaa aacgttcaca caattcaatg tcctttgcat attgatgata
     8881 aaaatcactt gccggatcgg gactgaatcg cccttgttcc tctaaaaagt catcccaagc
     8941 aacagcgcct ttgccacctt ccttagtggc tccttcaacc tgataggctg ctgtggctcc
     9001 gccaaaaata aaatcttctg gtaatttaag catgtacatc tctcctcaaa tctataatca
     9061 gttttcgctc tttctaactg attgcattgt aaacgcatcc aaaacatttg tcaacaaaaa
     9121 caaacacaaa taatcataaa aaccagaaaa cagcaataaa atctattgta acaaacataa
     9181 aaaccaccct aaatgtttac ttttgttcga attagattga ctgttgtccg tttatatgat
     9241 agattatcct taaattagag gtgaagacaa tgttgaaaaa agaaagattg cttactatcg
     9301 tagaaatggt caataaaaaa ggaatcctta ccgttaatga aatcatcaat caattagatg
     9361 tttctgatat gaccgttcgc agagatttag atgaattaga aaaatcgggc aagctgctgc
     9421 gtgtccatgg aggggctcaa agtctttcct acactttaga tcaagaactt tctcacactg
     9481 aaaaagccac tctgcagatt caagaaaaaa aagaaattgc tgccacagca gcctccttga
     9541 ttcacgaggg agaaacgatt ttcttaggac caggtacaac aatcgaacta ttggcgacgc
     9601 atttactgaa caagaaaatt cggatcatta caaataatta tccagttttt gatattcttg
     9661 ctcaagcaga tggaccagaa attctactga ttggcggtga tttccggaaa aataccggcg
     9721 catttgtcgg accgatcacc aacgacaatt taagaagact aaagtttaca aaagcattta
     9781 tcagttcaaa tggcgttcac aacgaagcga tctctactta cagtaccgaa gaaggagaag
     9841 cacagcagat cgctctaaat aattcccgta agaaatatct actcgttgat aataaaaaat
     9901 tcaatcgaga tgatttttat gtgttttata atttgcatga tttcgatctg ctcattacgg
     9961 atcgccagat ctcgcgggag gttcaagagc actatgcaca atatgtcgat ctgcaaattg
    10021 ctgattcaac cgaaaatacc actataggag cgtaactatg aaagggatta tatttgattt
    10081 caacggaaca atgtttttgg actctcatct gcatgaagct gcttggctgc atatgataca
    10141 tgatcataca aaagatggtt tatcagacga ggatatttta aaaaatattc atggtcggac
    10201 gaatacggag attttgaaac atttcatttc agagcatttg accgcctctg agatcgccgt
    10261 attgtcagaa gaaaaagaaa gctattatcg tatgctttgc ttgcaaaatg aaaacgagct
    10321 tcagttgaca aaagggcttg aagcgactct tgaccagctg actgaccaac agattccatt
    10381 aacgattgcc acggctactg tcaaagagaa tgttgctttt tattttgata ttttcgattt
    10441 agcccgctgg tttgactggg acaagattgt ctatgacgac ggctctttcc ctgggaaacc
    10501 gcaaccggat atttttctta aagcggcaga caaactgagc ttagctccgg ctgattgtct
    10561 tgttatcgaa gatgcttact caggattact tgctgccaaa cgtgcgggga tcggaacaat
    10621 tattgcaatt gatccattcg gtaaaaacaa aacagtattt gaacaggcac agctaaataa
    10681 agatggaacc atcaaagatt ttactggttt ttggacagag cacctgtctg ccacatccgt
    10741 taattcttaa acgattcaac taactaccaa agaaaagcaa ctgaaatgca cacctccttt
    10801 tataaaaaac tgccagtgaa acgacatgaa agatacactg ctatatcctt agtgaaatat
    10861 agcagtgtat ctttgacttt ttcattcttt taaccatatg acgcgttcat ctctatctat
    10921 aattaacatt aaaaatagtg ttttcacatt atttctcaaa ccaatcttaa aaatcatcag
    10981 gagtactttt ttgatatact tttattaatg ctgggcgtaa tggttaaaag atttccacta
    11041 gttcctctcc accaagctga gttgaaaaat gcttatctgt aaacaacata gacaaagagg
    11101 aaataaacgt ttccgtatct ttctctgtcc caaggatatt aattaatttt cctactaggt
    11161 aagtatcatt aaacttaaga tctgccttca catcaaaagt tttgataaaa gcttttatca
    11221 tgcgtacaat tttgtcttta tcgccataat tgatttcacc actcatatca atatagtcac
    11281 taatccctga agaaataatg gcaactgctt cttctttcgc cttatcagat aattcattta
    11341 atgactggga ttgattgatg acttcttgag ttttatctgt tacgtaatct ctcattatcc
    11401 tatcggcata ctccgcaaag taatcttgaa aaaattcttc tacagggtaa ccaacttcgt
    11461 tttcccaacc aggagaaatt tcattatcag tacttattaa atctattaaa aaccaacccg
    11521 ttct
//
LOCUS       DAYYHX010000083        11469 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000083 DAYYHX010000000
VERSION     DAYYHX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.084"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..3203
                     /gene="fss3"
                     /locus_tag="ACHA6Y_001614"
     CDS             15..3203
                     /gene="fss3"
                     /locus_tag="ACHA6Y_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8987711.1"
                     /translation="MLSLAVQSIVGAIAPTIAFADEITHPQTVTVELDLAHQYAVEGT
                     FSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKNPLPDMSDKAKLVS
                     VLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAIEAKINQAIADYQK
                     KPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVNGNQLIISPNANSK
                     ESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVEMEGSLKIKKVDKE
                     SGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTITEKSVPAPYTIDTT
                     PMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSLAGNTFAIRKDSPT
                     GEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTFKPVKVELNYVNQT
                     VALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTLFTVKDGKAVKWTE
                     NFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAPEGYTLDETKYPVS
                     IKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGFNDLTFKVSPLEGT
                     NEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGFQKITPLEIRSTFK
                     ENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNRLMLYDLPEEEDSL
                     TSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDVDATKAAQEKDKKA
                     KPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYESKEAYEAGDEPVA
                     KDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFDDVSITHDVLDGSK
                     EAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDTSKYPEGTSFTFKE
                     INYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVPSGSQESSPTVKAF
                     PQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            3315..3629
                     /locus_tag="ACHA6Y_001615"
     CDS             3315..3629
                     /locus_tag="ACHA6Y_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8987712.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3642..4016
                     /locus_tag="ACHA6Y_001616"
     CDS             3642..4016
                     /locus_tag="ACHA6Y_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8987713.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            4017..4370
                     /locus_tag="ACHA6Y_001617"
     CDS             4017..4370
                     /locus_tag="ACHA6Y_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM8987714.1"
                     /translation="MKNMEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQ
                     GLLEVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFL
                     KQLVETKQLPVVVND"
     gene            4439..5455
                     /locus_tag="ACHA6Y_001618"
     CDS             4439..5455
                     /locus_tag="ACHA6Y_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8987715.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            6049..7923
                     /gene="ltrA"
                     /locus_tag="ACHA6Y_001619"
     CDS             6049..7923
                     /gene="ltrA"
                     /locus_tag="ACHA6Y_001619"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8987716.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            8025..8405
                     /locus_tag="ACHA6Y_001620"
                     /pseudo
     CDS             8025..8405
                     /locus_tag="ACHA6Y_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            8450..9592
                     /gene="dcm"
                     /locus_tag="ACHA6Y_001621"
     CDS             8450..9592
                     /gene="dcm"
                     /locus_tag="ACHA6Y_001621"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFM8987717.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            9582..9871
                     /locus_tag="ACHA6Y_001622"
                     /pseudo
     CDS             9582..9871
                     /locus_tag="ACHA6Y_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10127..11311
                     /gene="mobT"
                     /locus_tag="ACHA6Y_001623"
     CDS             10127..11311
                     /gene="mobT"
                     /locus_tag="ACHA6Y_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8987718.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            11308..11421
                     /locus_tag="ACHA6Y_001624"
     CDS             11308..11421
                     /locus_tag="ACHA6Y_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8987719.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         3836 a         1972 c         2561 g         3100 t
ORIGIN      
        1 tatgtgtatt aactattttg agtttggctg ttcaatcaat tgtaggagca attgctccta
       61 cgattgcttt tgctgatgaa attacccatc cacaaacggt aaccgtagaa ctagatttag
      121 cacatcaata tgctgttgaa ggaactttta gtgatggtcg tccaatgtca gaagtcactg
      181 ttccgcatta tgcagtgtac aacggcgtta aacaagatat tttctgtgtt gaaccaggag
      241 tcccgattta caatgagttc actcctggat atgagaagaa cccgttacca gatatgtccg
      301 ataaagccaa attagtttcc gttttatgga aaaaagtagg tacagatgca gatactcata
      361 ttgtggcaca gaagatgatc tggcaagaag ttaatgggta tacccttcac tcaataaaac
      421 gatcagatgg tagtgccgta aatatcgcag ccattgaggc aaaaatcaat caagccattg
      481 ccgattatca gaagaaacca agcttccata atagtacggc taaaacagta ttagggcaat
      541 cgactactgt gacggatacg aataatctga atttgtcaga gttcgatgag gttgtggaaa
      601 ataccgcaaa cattgattat cgtgtgaatg gcaatcagtt gataatcagc ccaaatgcca
      661 attcaaaaga aagtggtgtg ttgacgctta agaaatctgc tggtaccgga acacctgtcg
      721 cctataaaaa ggctggccaa cagactttaa tggccggggc gatcgataag ccgaacactt
      781 atacggtcaa aattgatgtg gaaatggagg gttctttgaa gattaaaaaa gttgataaag
      841 aatcgggtga tattatacca ggaacggttt tccatttaga ctttggaaaa actttacccg
      901 caaaagacgt gaccaccgat aaagaaggca ttgcgacctt agatgagatt cctcatggta
      961 caaaggtaac cattactgaa aaatcggtgc cggcgcctta tacgattgat accacaccca
     1021 tgactgccac aattaaagca ggtgaaacca tttcagtgac ttcaaaaaat acgcgagaaa
     1081 aaggtcaaat tattctggat aaaaccggaa ttgaaacagg gagtgatctt tggaacgata
     1141 attactcttt agcgggcaat acgtttgcca ttcgcaagga tagcccaact ggtgaaatcg
     1201 tccaagaaat gaccacggat gaaaaaggtc atgcagaaac accaaaagag attgccaatg
     1261 cgttggaatt gggaacctac tatgtgactg aaaccaaggc tagtcatggc tttgtgaata
     1321 ctttcaaacc agtaaaagtc gaattgaatt atgtcaatca aacagtggct cttgttacca
     1381 gtaacgtaaa agggcaaaac caagaaatta ctggtgaaac cactttgaca aaagaagaca
     1441 aagataccgg tcatgaaact caagggaaag ctgagtttaa aagagctgaa tacaccttgt
     1501 ttactgttaa ggatggaaaa gcggtcaaat ggaccgagaa ctttaaacca gaattgttga
     1561 agggaacgaa agcttctgat gaaaccgtaa ctttgacttt agatgaaaag aatcaagttg
     1621 ccgttaaaca cttagcgatt aatgagtatt actggcaaga aaccaaagcg cctgaaggat
     1681 ataccttgga tgaaacaaag tatcctgtat ccatcaaaaa ggtggatgat agcgaaaaaa
     1741 atgctgtgat tactcgagat gttgcggcaa aagaacaagt gattcgcttt ggctttgatt
     1801 tctttaaatt tgctggatca actactggca ctgccgaaac tggcttcaat gatctaacct
     1861 ttaaagtgtc gccattggaa ggaaccaatg aaatcaccgg tgccgaagat gaagcgacca
     1921 cagcttataa cgagcaatta gggtttgatg gttatggcaa gtttgaaaat cttccttatg
     1981 gggattattt acttgaagaa gtagaagccc cagaaggttt tcaaaagatt acgccattag
     2041 aaatccgttc tacttttaaa gaaaataaag aggattatac caagagtgat tatatcttta
     2101 ctattaccga aaaagggcaa aagcaaccga ttaaaacggt aacggtccct tatgggaatc
     2161 taaccaatga gaatttctca gtcagtttga accgtttgat gctttatgat ttgcctgagg
     2221 aagaagatag cttaacttct cttgcgactt ggaaagaagg agataaagaa ttgactactc
     2281 ttgattctac cgagctagtc gataaattga gctataatct acatgaaatc aaagaagact
     2341 ggtatatcgt agctcaagcc atcgatgtag atgctacaaa agctgcccaa gaaaaagata
     2401 aaaaagccaa accggtggcg attgcggaaa caaccgcaac tttagcgaat aaagagaaaa
     2461 caggaacttg ggagattctg cataaattaa ctgtcgaaca agttttagac aagaccattg
     2521 tcttattcaa ctatgtgtat gaaagcaaag aagcctatga agctggtgat gagccggtag
     2581 cgaaggatgc tagcttgaac aatcaagcgc aaactgttaa ttgtatggtg gaacgtcatg
     2641 tttccatcca aaccaaagcg cacttagaag aaggttctca gacctttact catggtgacg
     2701 tggtagatat gtttgatgat gtatccatta cccatgatgt actagatggc tcaaaagaag
     2761 cttttgaaac aattctgtac gctttatttc cagatggtac aagtaaagaa atttggaaat
     2821 ctggcaagat tgattacgaa gtgaatgaca aagaatttac caaaaccgta cttgcggaaa
     2881 aagtggatac aagtaaatat ccagaaggta caagttttac cttcaaagaa attaactatg
     2941 acaaagatgg gaatatcaat ggaaaacaca atgaagattt gaaagaaaaa tctcaaactt
     3001 taacgcccaa aaaagttccg acaacaccaa gtacaccaga acaatctgaa atgccaactg
     3061 ttccaagtgg ctctcaagaa tctagtccta cagtgaaggc attccctcaa accggggaga
     3121 aaacttccaa tgtgctactg ttcattggct ttagcttgat ctttgcgacg gctggctatt
     3181 atttttggaa tcgtcggaac taaggtgata agatgaaaca tgtaaatagt agaaaacaat
     3241 acgcgcgacc gcctcctaaa aaagtggtcg cgctattttt gtattcaaaa ctaacaatga
     3301 aaagaggaaa aaatatggaa ttgaaatttg ttgtaccaga tatggtcgag acgttcggaa
     3361 aactaacata cggtggtgaa ggtgaagttc tatcggaagg gtatggtcga aatactacaa
     3421 ttgttggtcg aagttaccat ctgttttcaa ataaacaacg ggccgatgat attgaagtgg
     3481 tggtagctgc ggaagccggc gaaaaggatt ttgaccaaga tcaaccacta aaagctgtaa
     3541 atccccactt aattgccaaa ggctatgaaa ttgagaatcg tgggtttacc gattacgtgt
     3601 tgtatgttga tgatttagtg aaagcatagg aggaaaagaa gatgagatta acagaaggta
     3661 ttgtcgtaga ttcaggatta acctttggca agttgcgttt ttccgcatta cgtcgagaag
     3721 tacgcaagca aaatgaggac ggaacggtta gtaacgaggt aaaagaacgg acctataatt
     3781 tgaaatcctc cgcgcaaggt cgaatgatcc aagtcagtat tccagcaaat gtacccttac
     3841 gcgagtttgc ttatgatgca gaggtagagc tggtcaatcc aatcgtggat acggtcgcca
     3901 attatgtttt tcgggaagga accaccgtta attggtttat caaggcagat gatttagtct
     3961 tgaaacgaca actgaatcaa ggaaaaccta cgaaccaaaa tgaaggaaag aaataggtga
     4021 agaatatgga aattaatgtt gaacatattt tagattgtct cgatcaattt ggcaaaggtg
     4081 agctaacgga ggaacaactc actaacgcat taacctatga tgaaaaaatg tttctgatta
     4141 tgcatcaagg gttactggaa gtaggtaacg atgccaaaga agactttgat gtactggatt
     4201 ggttagggga agaagagtct ttctttatgg ttgtcgaggt aaatgaaagt ttatgtcggc
     4261 aggcagaatc tgttttggaa gaaatcgatg tggagatgcc ggatgccatc gaaagatttt
     4321 taaaacaatt agtggaaacg aaacaacttc ccgtagtagt aaatgactag cataactttt
     4381 tgaaattgcc caatgaaatc ctattgggct ttttcgactg gaggtggaaa ccatcaaaat
     4441 gtacaaagga catcgcatac gcgcaggaga tcaacacttg atttatcact ttgttcttgg
     4501 ctggctaatc tttttgttca ttggttggat gagcgtcttt tatttacaag aacttagaca
     4561 atttgatatt tctaaggtat cattttccac tagtgaaatt ggctggtcta tgaaggatgt
     4621 aatctgcttg ctaggaagtc tcgctctttc aggaagcatg atgttacttt atatacactt
     4681 ttttcaggat cattggcgaa gtctttggca tcgacaaaag ttagctcgga tgattctaga
     4741 aaaccattgg tatgaagtaa agcagactca aggtgaaggt tttttcaaag atttgaatag
     4801 tagttggacc aaagagagta tcagttactt ccccaaaatt tattatcgaa tgaaagatgg
     4861 cttactttct attcgcgttc aaatttcact gggaaaatat caggatcagc tattgaagtt
     4921 ggaaaataag ttagaaagcg gactgtattg tgagttagta gaaaaagaac tcaaagactc
     4981 ctatgtggaa tacaccttgt tatacgatat gattgccaat cgaattggga tagaagaaat
     5041 agtggcagaa aatggggcgt tgcgattgat gaaaaatcaa gtatgggcgt atgattcctt
     5101 gccgcatatg ttaattgctg gcggcacagg tggtgggaag acctatttct tactcaccat
     5161 tattcaagca ctattgaagt cagatgcaga attgtttatc ctcgatccca aaaatgcgga
     5221 cctcgccgat ttaggtacga taatgcctca cgtatactcc caaaaagaag aaatttctga
     5281 atgtgtggaa gatttttacg aacgcatgat agctcgtagt aggtcaatga aagaaatgcc
     5341 caactacaaa acaggagaga attatgcgta tctcggactt ccgcccaact ttttaatctt
     5401 tgatgaatac gtggcttata tgggtgcgaa tcgttttcct acaagcatcg aatagatgtg
     5461 aaatggaagc ctattgagat tatcaatagt gacaactaat gttttgagag atggaatgaa
     5521 agccttcaag ttgagctgaa acatccccgc taatactcct acatgctcta atcaatgaag
     5581 tagattggtt ttggtatgaa gctaggtgaa gtcgtctgaa atctgcctaa gtgcttgagc
     5641 ataaggctga ctatagggcg ggcggctgaa aaagataact atggtaagaa tgtctgaaaa
     5701 tgactgacga atctgcgaat gtacgggtct aaaaggctga tcttattgaa agataggaac
     5761 atacggacgt atggtgagat accgacaagt aaggttccat gatatgaaaa tcgggatagc
     5821 taacgaagaa gctataattt cacaggctca tagtagatac ctaaggttat acatgatagc
     5881 taaaaacatt ggaacgatgt aagcagaaaa ctatcaatta aatgactact tctagatagg
     5941 tgcatataag taataacgaa attgctttgt ttctgtgaaa gtaagggcac agtaccgatg
     6001 aagcgtctaa caaatgtgga gggatagccc taagtctatt taataacaat gaatgttgaa
     6061 acagatgaag ttcaaggatc tggtaagaac gtgggaaaat ccgttatgga ggtgccacgc
     6121 ttgaacacaa aaatacgtta ctgggaatat tatgatctac aagaaacatt cgatcagcta
     6181 tttactcaaa gtagaaacgg gaaaaagttt tatcaattat atgagttaat catttctgaa
     6241 aataatattc ttttagcata ccgtactatc aaggcaaaca aggggtcttc gacaccagga
     6301 acggattcct tcacaattga taactataag gaaatgaacc aagcagagtt tatccatctt
     6361 attctcagtc atttagaaaa ttacaaacca aaatccataa agcgagtaat gatacctaaa
     6421 cccaatggtg agaaacgccc attaggaatt ccatgcatga ttgatcgaat gatccaacag
     6481 atgttcaagc agatactgga gccaatatgt gaagctaagt tttatgaaca tagctacggt
     6541 ttcagaccat tgagaagtgc aaagcatgct ctaggtcgca ttatgtatct cataaacatc
     6601 agtaaaatgc actatgcagt agacattgat atcaaaggtt tctttgataa tgtaaaccac
     6661 cgtttactga taaaacaatt atggaatata ggaatttgtg acaaacaagt cttagctatt
     6721 ctttcaaaat ctctgaaatc acccattcaa ggagaaggta tcccgagcaa aggaacgatc
     6781 caaggaggta tcatttctcc actattatct aatgttgttc taaacgactt agaccattgg
     6841 gtctcaaagc agtggcatac ctttgaaaca aagtatccct atactaaagg ttacaataaa
     6901 tttcgtgctc taagagatac aaatctaaag caaggctata ttgtcaggta cgcagatgac
     6961 tttaaaatca tgactaatga ttatccgaca gcattaaaat ggttccacgc tgtcaagctt
     7021 tacctaaaag acagacttaa attagatatt tccaatgaga agtctaaaat cgtcaatttg
     7081 cgtaaatgta agtcggaatt tttaggattc gctatttgcg tgaaacaaaa agggaaaaaa
     7141 tgggtttgta attcacgtat ttctaacaaa aaaaaggatc agattaaaga agagattaaa
     7201 caaagaatca aagatattca aaagagtcct acagcacaaa atgctctcct gtttaatgct
     7261 ctcgtgctcg gtgttcataa ttatttcagg tatgccactc atattaattt agatttgaat
     7321 aggatgaact acgttctttc attcactact tacaaccgat tatcaaattg tagtacaaga
     7381 tgttacccca acaaagcttc cccaacttac aaaaagtttt actcaacaac gatgaaaacg
     7441 tataaagtag ctggagtcta tttgtaccct ctttgcgacg tatctacaaa aacaatttac
     7501 ggatttaata cggaggatac accgtttact ccgtttggtc gtcaaagatt ggagcacaaa
     7561 agcctacagt ctcttgtgtt ccaagaatta agaaaattga tggaatcgaa aataccgaat
     7621 agaacggttg agtatttaga tagtagaata tctcgttact caatgaaatc aggcaagtgt
     7681 gagattacaa aacaattttt gccagcaaaa gccgttcact gtcatcacta tttgccaaaa
     7741 agtcttggtg gagacgacaa attcgataac ttgagaatta ttcataagga tatccatctt
     7801 ctgatccata caacaaataa aatgataatc gatcactacg tgcatgaact gaaactggtg
     7861 ccagagcaaa tagcaaaaat caacctttat cgaaagatgt gtaatctgca aaacattcaa
     7921 tagaaaataa atagatggaa cgccgtatga ggtgaaagtc tcacgtacgg tgtggagcgg
     7981 gggaaaagat ggagattata tcaaattctt acctatcgct atagatgtta acgacaaaag
     8041 aaagtgcggt gattttgaat aagcttaaac aaatcgtgat gttaggccgt caatctggtt
     8101 tctttctgat tctagcttgt caaaggccgg atgcgaaata tctaggagat gggattcgcg
     8161 atcagtttaa cttccgtgtg gctttgggtc gtatgagtga gcttgggtat tccatgatgt
     8221 ttggagaagt cgataaaaat ttttttatga aacgcatcaa aggacgaggt tatgtagata
     8281 cagggggcag tgtgattagt gagttttata cgccacttgt accaaaaggg tatgatttct
     8341 taagggaaat tagcaacatt gttggattaa ccgttcatac ggaaagagaa aataatagtg
     8401 tgtagcctag gaatatcaat aatcctgggc ttttttattg aggtgattta tgacattttt
     8461 agatttattc gccgggattg gtggctttcg cttaggtatg gaacaagccg gtcaccagtg
     8521 tatcggtttt tgtgaaattg atgaatttgc tcggaggagt tataaagcaa tccatgatac
     8581 aaggaaggag gtggaaatgc atgacatcac aagtgtatca gacgaatttg ttcgatccct
     8641 cggaccagtg gacatccttt gcggcggctt tccgtgccaa gctttttcaa ttgcgggaaa
     8701 gcggcaagga ttttccgata ctcgaggtac tttattcttt gaaatcgctc ggttcgccac
     8761 tcttctccaa cctaagtttc tattccttga gaacgtccgg ggattgctca atcacgaagg
     8821 aggggctacg ttcgagacga tcctccgaac gttggatgga ttggggtatg atgtggaatg
     8881 gcaagtgctt aactcaaagg cctacgttcc ccaaagccgt gagcggatct tccttatcgg
     8941 acattctcga gatgcatgta ccgaacaagt atttcctatc attggatcgt cttcaacatc
     9001 tgatcaaaac atcagaaact tggtaaatat caatccttcc aatcgaggaa tgggtggcca
     9061 agtctatggt tcagatagcg tagcaccaac cttgaccggt gatgaaggaa ttaaaattgc
     9121 cttaccggta aaggatggga tttctgtagc gggtatgttg tcgggaaatt ttgaacaagg
     9181 caataggatt tatgaggtaa ccggaacagc ccccactttg tcaacgaaac aagggggaac
     9241 gaagattatg attcggaaaa atgaaacaga atatcaagag gtgaagccag gggacagtgt
     9301 gaacctggct tttcctaatt ccacaagtcg ccgaggtcgt ctgggaaaac aaagtgtgca
     9361 taccttacta acaggagacc aacaagcggt agtcacggat cagtaccaga ttcgaaagtt
     9421 aacacctagg gagtgctggc ggcttcaggg ctttccagat tgggcttttg atcgagcttc
     9481 tcaagtcaat tcagatagtc agttgtataa acaggccgga aactcagtaa cagttccggt
     9541 catttttgat attgctagaa gattaaaggg ggtaaaagca tatgatttat gacgagttga
     9601 ttggagaaat ctactggatg attggcaaga ttcaggcaga tccgaaattg gaagaagaac
     9661 tgtaccggct caattttgat attcgcaaaa acggggtgaa agtccaggtg attcgtatgt
     9721 agtggatgag gaaacggatg ctcgaatgga gctcaatcaa gtcatcggtg aattggaacg
     9781 aattgcggac cttgccaaag aacctgatat ccgccaatac ttgtttgaga taaaagcaga
     9841 gttggaaatt gagggtatca ccgcggagta atagggaaat aacccactga taggcactga
     9901 ccgcggggga tttgcgaagc agaaatcctc cgtggtgctg tgcctgttca tggacggcgg
     9961 cagccggcca tgatgtctca ccccccgtat ctaacagggg ggtacaaaaa cacaaaaaac
    10021 aagcaagtaa ccataaaaaa tgcttgttgt atcaaggata ttaagtaatt tgtataggtg
    10081 ttaactcatt tggaaaagtt aacaccttga atttggaggt gaaaacttgg cgcaacgaaa
    10141 tttagattat cggttattaa aagaacgacg caacgaatat ggcgtttcac aaaataaatt
    10201 agctactgct tgcggactta gccgcccata cctaaatcag atagaaaacg gtggcgtaat
    10261 cgcctctaca aagaccatga gaaaaatttt tgatcaactg gaaagtttca atcccgattt
    10321 acctttaacg ttactatttg actatgtaag gattcgcttt cccacaacgg atgtgcggaa
    10381 aattattcaa gaaattctcc atttgaaatt cgagtatatg cttcatgaag attacgcctt
    10441 ttactcctat caagaacaat atgtcatggg cgatattgta gtgatgttat ctcatgaaga
    10501 agataaaggt gtccttttgg aattaaaagg tcgtggttgc cggcagtttg aaactttttt
    10561 actcgcccaa aagcgtagct ggtacgactt ttttgaagat tgtctaaaag ccggtggcgt
    10621 gatgaaacgc ttggatttag caatcaatga tcgagtgggg ctactggata ttcctgattt
    10681 aacgaaaaaa tgtcagaagg aagaatgtat ctccttgttt cgtaccttca aaagttaccg
    10741 ttccggggaa ctgttgaaag ctgatgaaaa ggatggtatg ggcaatacct tatatattgg
    10801 cagcctcaaa agcgaggttt atttttgttt gtacgaaaaa gattatgaac aatatatcaa
    10861 gttagggatt ccattggacc agaccgaaac gaaaaatcgt tttgagattc ggttgaaaaa
    10921 tgaccgtgct tattatgcga ttcaagattt gttaaaaggt cgtagcattg aaagtaccac
    10981 gttttccatt atcaatcggt atttgcgatt tgcggataaa gtagatggaa aacggcgaac
    11041 caattggccc ttgaatgaac aatggggtcg ttttattgga cggaatcgga aagagattca
    11101 attaacttct gaaccgaaac cgtataccat tgaacgaaca ttaaattggc taggtagaca
    11161 agtcgcccct acgtggaaaa tggctaagga gttggatcga ttgaatcaaa caacgtatat
    11221 ccaggatatg gtacagaatg cgcgactctc ggaccggcat aaaaagattt tggagcaaca
    11281 aagtatggca atcgaaaact tgattgtatg aaaagaggaa ttttgatgga aattgtaaaa
    11341 gatatgttat tggtacttgg tggcagtatg cttggtgtta ccgtgatgtg cttgatgaat
    11401 gcaagtgcag cagcggattg agcaatggaa aagaaaggaa agtgacaaaa tgaatttcgg
    11461 acaaaattt
//
LOCUS       DAYYHX010000084        11466 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000084 DAYYHX010000000
VERSION     DAYYHX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.085"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..648)
                     /locus_tag="ACHA6Y_001625"
     CDS             complement(163..648)
                     /locus_tag="ACHA6Y_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM8987720.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    738..1083
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1128..1769
                     /locus_tag="ACHA6Y_001626"
     CDS             1128..1769
                     /locus_tag="ACHA6Y_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8987721.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1788..2423
                     /locus_tag="ACHA6Y_001627"
     CDS             1788..2423
                     /locus_tag="ACHA6Y_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8987722.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2441..3256
                     /locus_tag="ACHA6Y_001628"
     CDS             2441..3256
                     /locus_tag="ACHA6Y_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8987723.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3575..5011
                     /locus_tag="ACHA6Y_001629"
     CDS             3575..5011
                     /locus_tag="ACHA6Y_001629"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8987724.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5200..7647
                     /locus_tag="ACHA6Y_001630"
     CDS             5200..7647
                     /locus_tag="ACHA6Y_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8987725.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7877..8944
                     /gene="rlmN"
                     /locus_tag="ACHA6Y_001631"
     CDS             7877..8944
                     /gene="rlmN"
                     /locus_tag="ACHA6Y_001631"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8987726.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8934..9140
                     /locus_tag="ACHA6Y_001632"
     CDS             8934..9140
                     /locus_tag="ACHA6Y_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987727.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9137..10240)
                     /locus_tag="ACHA6Y_001633"
     CDS             complement(9137..10240)
                     /locus_tag="ACHA6Y_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8987728.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10792..11340
                     /gene="aac(6')"
                     /locus_tag="ACHA6Y_001634"
     CDS             10792..11340
                     /gene="aac(6')"
                     /locus_tag="ACHA6Y_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8987729.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3806 a         1940 c         2478 g         3242 t
ORIGIN      
        1 aataaatatg gatttggcta actatggaag aatactcgta ttagttttca gcgttcatac
       61 tagttgttac attgtttgat tggccaaaag aatttctgat atattttgtg ctaaataaag
      121 aaaaagacgc ttgaattggg aaataagaaa actactctat cattacaaat tttcataaac
      181 tggcagatgt tctaagtaaa caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt
      241 agtggtattg atatttggat gaaagcctac ggtatcttga cgatcaatag cagcatcgat
      301 cacgacttgg atttttttct ctttgtcgtg catcaatgct aatggagtca ctgtacctgc
      361 tggaacgcct aggaggtcag gaagctgttc ttcagaaaca aaagataagc gcttttcttt
      421 taattgttca gctagttgtt ttaaatcggc gtgcttttca tcaggcaaga tgacaagata
      481 gatttgtttt ccttttcttg atttcaataa tagatttttt acttgcggac caggtaggga
      541 aaaagggaca tttttgactg acgtaatcgc aggatgatcg actttttgaa aagagatacc
      601 cagttttttt agtagttcat aagcttcttg ttcggttgca cgactcatcg cgcatcaccc
      661 tccttttttt attagtgtat cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat
      721 tacacgtaaa gttaaaacaa atgacatcga aaagaagagt agccgttagg tgtttttaga
      781 gagtctctgg ttggtggaaa gaggcagcag ttaaccgtga aacacatctt ggagtcgatt
      841 ttctgaagcg tatagtagga aaatccgggg agcccgttac agctgcggtc gttgctagat
      901 gaccagataa ggctggcttt tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt
      961 aacgtccttt gatcttcgga tcaaaggact ttttgttttc atctgaacgg caacgactgt
     1021 gtgaggtttt ttctgcaagg aaaaaataaa cagaggtgga accacgtata atacgtcctc
     1081 ttggcatttg ccaagaggat ttttttattt taaaggagga aaagaagatg gagtatttga
     1141 ttgaagttat gccgcaatta ttagtaggag cactcacaac attgaaatta tttttattta
     1201 ccttgattgg ctcattacca ttagggattg ttttagcatt aggattgtct ttgcatttta
     1261 agccgctacg ctacttgctt caaacatatg tatgggttat gcggggaaca ccgctgttgt
     1321 tgcaactgat ttttattttt tatgggttgc cgacgattgg gatcgttttt gatcgtttcg
     1381 aagcagcact cattgccttt atcttaaact atgcagctta ttttgcagag atctttcgtg
     1441 gagggataca atcgattcct caaggtcaat atgaagcagc gcaagttttg caattaagtc
     1501 cctttcaaac ggtgacgaaa atcgtcatcc cacaagtgac aaagattgtt ttaccttctg
     1561 tagggaatga agttatcaac ttggtcaaag attcttcctt gatctatata ttaggtttag
     1621 gtgacgtaat gcgtgctggg aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc
     1681 taggcgtggc aatcatctat cttacattga ctggtattct aacaatcgta atgaaacaag
     1741 ttgaaggaaa attaaattat taccgctaac tgataggagg aagaaatatg attgaattga
     1801 agaaagtgaa caaagcattt ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc
     1861 cagatgggaa aacattagct atcgtaggtc cttccggtgg cggtaaaacc acgcttttac
     1921 gtatcttggc tggcttagaa agttctgaca gcggagaatt ttatttagac ggtgtaccgt
     1981 tcaacccatt cgagcttaaa atgaaagaac aagttgtagg agtcgtattc caagacttcc
     2041 agctatttcc acatctgaat atttttgaaa acattacctt agcaccaaaa atggtattga
     2101 aacagacaaa agaagaatac accaaaaaag cagaagaatt agcggcttca ttagggatta
     2161 gtgatttatt gaatatctat ccttatcagc tttctggcgg acaaaaacag cgggtagcga
     2221 ttgccagagc attggctatg aatccacggg tattggctta tgatgaacca acaagtgctt
     2281 tagatcctga attacgccag caagtcgagc gtttgatcct atcattgaaa gaagatgggg
     2341 taacccaaat tattgtcacc catgatttag tatttgcaga aaatgtcggt gatcaattac
     2401 taaaagtttc cccaattcat taagaaagga gaagatagag atgaaaaaaa ccagttttct
     2461 attcgctttg atcgcaggtc ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc
     2521 agataattgg gcgactatca acgaagagaa aaaaatcgtt ataggtttag acgatacatt
     2581 tgttcctatg ggatttagag ataaagacgg gaagctcaca ggatttgata ttgatttagc
     2641 caaagcagtc tttgaacaat atgggattac ggtagacttt cagcccattg actggtcaat
     2701 gaaggaattt gaactgaaca atggaacgat cgatttgata tggaacggct attccaaaac
     2761 gccggcaaga gaaaagaaag tgcaatttac aaaaccttat atggaaaacg atcaagtgct
     2821 gatcacacct aaagaatcag gaatcacaaa ctttggacaa atgaataata agagattagg
     2881 tgcgcaaaat gggtcatctg gttatgatgt atttacgaaa caaccagaag tattaaaaga
     2941 gatagtcaaa aaccaagatg ctgttttgta tgatagtttt aatgaagcat tgatcgatct
     3001 gaaaagcaga cgggtcgatg gtctgctgat ggataaagta tacgcagact attatttgaa
     3061 acaaagaaac gaactagaag acttcaacat aacaaaaggt tcttatgaga gtgaggattt
     3121 tgcagttggt gcacgcaagt cagatagtac attagtgaaa aaaatcaatg aagccctaga
     3181 tcaactagag aaaacaggtg aattccaaaa aatatccgat aaatggtttg gcgaagatgt
     3241 tacgccgaga aattaacaaa taggggactg tgacaaaaga cgtgtcacag tccctttttc
     3301 cgaataaaca gtgttctcct gccaactcct tgtgactcgg agctgaatgg caggctcaca
     3361 acctcttggt aaacggtgtt caaaagccag tttttcggta ttaagccaaa tttatgcaaa
     3421 aatgtagaaa gccattttca caaaattttt cttactactc gaaactaagc gactttttca
     3481 caacctcctt tacataattt tattttgttg tggaaggggt ttcgtgttat tctaaaagag
     3541 gaacgattga aggttatagg aaaggaagag atgaatggaa cacaaagaac ttacctcatt
     3601 tccaaaagat tttctgtggg gctctgcttc tgcagcgtat caagtagaag gagcttggca
     3661 agaagatggg aaaggagaat cagtctggga tcgatttgta cggatacctg gaaagacatt
     3721 taaaggaacg aacggtgatt tggcagtaga ccactatcat cgttataaag aagatattgc
     3781 tttaatgaaa gaacaaggtc tgaaagctta ccgcttctct gtagcttgga cacggatttt
     3841 tcctaatgga cgaggagaag tcaatcaagc tgggctcgct ttttatgaga gattgatcga
     3901 cgaattgatt gaaaacgata ttgaacctat gttgacttta tatcattggg atcttccaca
     3961 agcattgcaa gatgaatata atggttggga atcacgtcag atcattgaag atttcactca
     4021 ttatgcagaa acattatttg aggcattccg cgggaaagtc cattactgga ttagtttgaa
     4081 tgaacaaaat atcttcactt cattaggtta tttgttagca gcacatccgc caggtgtgac
     4141 agacccgaaa cgaatgtatg aagttaacca tattgctaat ttagcaaatg catctgtcat
     4201 caataaattt cacgagttag aaatgcctgg aaaaatcgga ccaagctttg cgtatacgcc
     4261 aaattatcca attgacagta atcctgaaaa tgttctggct gctgaaaatg cagaagacct
     4321 gatggccaat tattggatgg atgtctatat gtggggaaaa tatccgattg ctgccatgag
     4381 gttcttagaa gaaaaaggat gggcaccaac gattgaagct ggcgatgcag aattgctaga
     4441 atctgctaaa ccagattttc ttgggatcaa ttactatcaa acggcgacga atgcattcaa
     4501 tccattaaac ggagttggtg ctggaaaaat gaatacgaca ggaaaaaaag gttcatcaga
     4561 agaaactgga gttcctggaa tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa
     4621 ttgggactgg gagattgacc cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg
     4681 ttaccgcatg ccggtactga ttacggaaaa tggcttagga gagtatgaca agttgacaga
     4741 agataaacaa atccatgacg actatcgaat caattactta caaagccacg tcaaagccat
     4801 caaagaagca atttcggatg gagcagaagt cctaggctat tgcacctggt cttatacaga
     4861 tttgcttagc tggctgaacg gttatcaaaa gcgatatgga tttgtatatg ttgatcaaga
     4921 cgaaacacaa gaaggatctt tggaacgata taagaaagac agttattact ggtaccaaaa
     4981 actgattgaa gaaaatggag agaaccttta gttcacatac ttaaaacaag aaggtacggc
     5041 tggatatggc cgtgcctttt cttgataaaa tcatttagaa attaagaaaa actgtaaaat
     5101 aagctgaaaa atgaaagaaa gcgtcagttc tttcaaacaa aactttagtt cccttacttt
     5161 tttttgtata atgaaacttg atgaaatgag ggataataaa tggcacaaaa gaaacggcct
     5221 actaaaaaaa ggaaaaagac gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg
     5281 ctgacgattg gtgttgggtt tgccttgatt ttatttgctg tcttcggctt tttaaaatta
     5341 ggttttttag gaatattgat tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa
     5401 atcctctgtc ttttattagc cgtattagga ttttggattg tcattaagaa tacagagttt
     5461 tcaattggta aaaaccgacg ctggtttggc ggcatactct tttatttcgg tatcttatta
     5521 ttgttacatg cccatttatt tggcaaactc catactgggg aaccaaatat catggggacg
     5581 acatgggatt tgttagccag tgatataaaa caaagtcaag tagacaataa cgttggtggt
     5641 gggatgattg gagcaatcct ttatcatttt acttacttct tgattgcaca gcctggaagt
     5701 tatttagcag cagtgctatt actggcagga ggagcattcc tgttcagtaa tctggaaggc
     5761 tatcagctgt taaatggtat ccaatcaatt ggagaacgag tacaggaatt attggaaggt
     5821 gatcctgcca agcaagctcg aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca
     5881 gaagcgaaag aagcaaggcg cttagctgca aaagaagctg cagaaaaaga agctgcagaa
     5941 tatgagaaga aaaaagcggt gagccagcaa agggaaaata ctgctgatga atggcaggaa
     6001 ccagaaaata cagagccgga gcagctaagt tttgttccaa tcgacagttt tcaagaaaat
     6061 atccatcctg cgaatctaga gaaaccagta ccagatacac caaaacagac caatacagca
     6121 gaaggttttt cagatgagtt gccagaggat gacggtacct cattagaatt tgagattgaa
     6181 gcagaacagg aaaatcaaga ctatgaatta ccgacagttg atttgcttga ttctatacca
     6241 actgtggatc aaagtgatga gtataaaaaa attgaaaaaa atatcggcgt attagaacaa
     6301 actttccaaa gtttcggtgt cgatgccaag gtcgtcaaag ccagcttagg accatccgtc
     6361 acaaaattcg aagtccagcc ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca
     6421 gatgatatcg cgcttgcttt agcggcgaaa gatgtccgga tggaagcacc gattccaggt
     6481 aaatcactga ttggtattga agtaccaaat ggaaagatca gtatggtttc ctttagagaa
     6541 atcatcgaag cacaaccaaa ccacccagat aaattactag aagtaccttt aggacgtgat
     6601 gtttctggac gggtacaaac ggctgatctt tcgaagatgc ctcacttact cgtggctggt
     6661 tcgactggta gtgggaagtc agtagcaatt aatgggatta tcacaagtat attaatgcgt
     6721 gctaagcccc atgaagtaaa actgatgatg atcgatccga aaatggttga attgaatatg
     6781 tacaacggca tacctcatct gttgacgccg gtcgtgacga accctagaaa agcagcgcaa
     6841 gccttacaaa aagtagtaca agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga
     6901 gtaagaaata tttctggtta caatgaattt gttcaacaga aaaacttgga aaacggaacg
     6961 aaacatccaa ccttaccatt catcgtagtg atcgtagatg aattagctga tttgatgatg
     7021 gtagccagca atgaagttga agatgcgatt attcgtttag cgcaaatggc tcgggcagcg
     7081 gggattcata tgatcctagc tactcaacgt ccaagtgtgg atgttatcac aggaatcatc
     7141 aaagcaaatg taccttccag aatggcattt gcggtatcaa gcggtacaga ttctcggacg
     7201 atcattgatt ctaatggggc agaaaagctc ttagggcgag gagatatgct attcttgcca
     7261 atgggtgaga ataaaccgat ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa
     7321 cgggtcgttc aatttgttac agatcagcaa gaagctcatt atgaagagaa aatgatgccg
     7381 acagatgaag ttgaaacgac aggtgctcca gaacagccac aagatgagct gtttgaagaa
     7441 gcaaaagcac ttgtcgttga aatgcaaacg gcaagtattt cccttctcca aagaagattt
     7501 cggatcggtt acaaccgagc agcacgttta gtagatgaac tggaagctca tggagttgtt
     7561 ggcccgtcag aaggaagcaa gcctcggaaa gtattcattg agcaggaaga aacatcagaa
     7621 acgttgccgg acgataatga catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa
     7681 ccaataaaaa gacaaggcat gatgatacca aaagaaaaaa tggttttttt ccatcttttc
     7741 taatacggta tgatgtcttg tctttttagt ttttgtgaga ttaaagttct attcgattta
     7801 tttgtgaagt attgcattaa ggaaatgaat catgtatagt atgtagcgaa actttgagaa
     7861 aaagagggaa aatactttgg aaaaacagtc catttatggc ttgacgaatg aagaattaat
     7921 caattggttt atagaaaacg gcgagaaaaa attccgagct gcccaagttt gggaatggtt
     7981 gtatcaaaaa cgtgtaagta atttcacaga aatgacgaat ctatcgaaac aattgatcga
     8041 aaaattatcg gagcatttta tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc
     8101 ggatggtaca gtaaaatatt tgtttgaact ccctgataaa aacatgattg agactgtact
     8161 gatgagacaa gaatatggct tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg
     8221 ctgtacgttt tgtgcaagcg ggctgctcaa aaagaaccgt gatctgactg ctggagaaat
     8281 tgttgcacag atcatgatgg tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt
     8341 ttcacatgtc gttgtaatgg ggatcggtga gccatttgat aattacgaca atgtaatgga
     8401 ctttttgcat atcatcaatg atgccaaagg attggcaatt ggtgctcggc acattacagt
     8461 ttccactagt ggattagcac acaaaatcaa ggaattcgct aataacggct tgcaagtcaa
     8521 tctggctatt tccttgcacg cgccgaataa tgaggtgcga acatcgatca tgcggatcaa
     8581 ccgaagtttc ccaattgaga aattgatgga agctgtggat gagtatttag agaaaacaaa
     8641 ccgtcgaatc acgtttgaat atatcatgtt gaatcaagta aatgatagac cagagcatgc
     8701 gcaacaatta gcagatctat taaaagataa gaaaaaatta gcatatgtca acttgattcc
     8761 atataatcca gtcagtgaac atgatcaata ttctcgcagt ccaaaagctg atgtattgag
     8821 attttatgat gtattgaaga aaaacggcgt aaactgtgtg atacgtaaag aacatggtac
     8881 agatattgat gcagcttgcg gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa
     8941 ataatcgtat cctactcgtt ttttcaatta ccataaaagg agcagttttc ttaattaagg
     9001 aactgttcaa cttaaataag agaaaagatt ctatagcgaa agctataaag ctttttttga
     9061 caagaaaaac cctcatcaag caaagaaatg tcctcaattt gatgacagcc tgcttgatgg
     9121 gggttttagc aattatttaa ctgattttct tatatccttg ctggctttct tgtgtagcat
     9181 gataagtttc tacgtcaaga atggcctgtt taaatttttc tcgggtgatt tcataaggca
     9241 catgtttaat atcatttgtt gccatggctt tatctaagat tgcttctaaa tcggtcgtga
     9301 cattgagatt catgtcggtt aacgatactg gaagttggtt ctttttcata aacacataga
     9361 ttttatcaaa caaatcgaac tgctgatcca tcatcaataa ggtcagtaca ccataactga
     9421 cagatgctcc gtgaaggtat ccctccattt tttctagaac agtcgtaccg taatagaatg
     9481 agtgtgccat agcagaatta tagttattgt taactaaaac agaaaccaat ccggtagtaa
     9541 caatgatatc cgaaacgact tgttcaattg catatgaggg acgatcgtcg gcactatctt
     9601 tcaatgcttg cgtaccataa gtcaagagac gatcaatgca catttttccg atttggattc
     9661 ctaatctatc cgtgtagcct agatcatcgc cacgagcaga aaaagtagat tcgacttctt
     9721 tggataaagc atcgccaata cctgcccata agtatttatc aggtgcttcg gcaataattg
     9781 ctgtatcaat gaaggtgtgt acaggtggtt ttgaaggaaa gaagagtccg tccattacgc
     9841 cttcttcgtt atagatgaca cataccgcag tgacgggcga acagttcgaa ccaatagtgg
     9901 gaaaggtgaa gactggttta gctagttttt caccaatgac ttttacagta tcgcaagcac
     9961 ggccaccacc gactgcaaac aggacgtctg ctttttggac actttcatgc tggatcaact
    10021 gttcaatatt tccataagtt gaattacccc cgtaccagag actggcgaca acctctattt
    10081 cctgtgtcaa agctactttt attttgtttt ctgcctttga aagtgcagtc ttccctccaa
    10141 tcagcactac ttttgtccca tattctttga cgattccatt tgctttttca tacgcatctg
    10201 ttccaatggt ataatttggt aagaaaacac tttgactcat attcatttcc ttctttcttc
    10261 gttgaaacgg gttaaaattt taaagagtga tcgatctgac tgcggctgta gatggagtag
    10321 aggtgaacct aagacaggat aagtgatgaa taaggtggca tgaacacctt gaaatcatca
    10381 ataagtatcc taaaaacgaa caagtacaaa aacattgaaa acgacaataa tgaatttttg
    10441 ttcctcttag tatttctacc gattcccttt gcttttttgt aattatctaa aaattctaaa
    10501 aattaagatt attatatgaa aacaggtttt atttgtcaat tcataattgg ttttttttaa
    10561 agcaaaagat aatctatatc atgatgaaca aacagagttt tttcctccaa ttgatggctt
    10621 tcctgatctt tcctattttg aataaagtgt ttctgtataa ttttttattt tataagtaag
    10681 ataaataatg aaagaataga gatactgatt ggtagctaaa gatttttgag cgagaacatt
    10741 tcgttttttc aaaaggactg ttagaataag caagaacaag ggggaagacg tatgataatc
    10801 agtgaatttg accgtaataa tccagtattg aaagatcagc tttctgattt actgagactg
    10861 acttggccgg aagaatatgg agacagctcg gcagaagaag tagaagaaat gatgaatcca
    10921 gaacgaatcg cggtagcagc ggtagaccaa gatgagttag taggatttat tggtgcaatc
    10981 cctcaatacg gtatcacagg ttgggaattg catccattag ttgtagaaag ctcccgacga
    11041 aagaaccaaa taggtactcg attagtcaat tacttagaaa aagaagtagc ttccagagga
    11101 ggaatcacga tttatttagg tacggatgat ttagaccatg gaacaacgtt aagtcaaacg
    11161 gacctgtatg agcatacatt tgataaagtg gcttccatcc agaaccttcg tgaacatccg
    11221 tatgaattct atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc
    11281 tgggacaaac cggatatttg gatggcaaaa acgattattc ctcgaccaaa ttctcaataa
    11341 taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat
    11401 tacttcttga atttttgtga aattccaact tatttccgaa gaagctactt ttggaacacc
    11461 gtgtac
//
LOCUS       DAYYHX010000085        11287 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000085 DAYYHX010000000
VERSION     DAYYHX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.086"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            218..886
                     /locus_tag="ACHA6Y_001635"
     CDS             218..886
                     /locus_tag="ACHA6Y_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM8987730.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1086..1406)
                     /locus_tag="ACHA6Y_001636"
     CDS             complement(1086..1406)
                     /locus_tag="ACHA6Y_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987731.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1443..2825)
                     /locus_tag="ACHA6Y_001637"
     CDS             complement(1443..2825)
                     /locus_tag="ACHA6Y_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8987732.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2981..4168)
                     /locus_tag="ACHA6Y_001638"
     CDS             complement(2981..4168)
                     /locus_tag="ACHA6Y_001638"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM8987733.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4175..5206)
                     /locus_tag="ACHA6Y_001639"
     CDS             complement(4175..5206)
                     /locus_tag="ACHA6Y_001639"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8987734.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5252..6676)
                     /locus_tag="ACHA6Y_001640"
     CDS             complement(5252..6676)
                     /locus_tag="ACHA6Y_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM8987735.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6851..7756
                     /locus_tag="ACHA6Y_001641"
     CDS             6851..7756
                     /locus_tag="ACHA6Y_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM8987736.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7847..8524
                     /locus_tag="ACHA6Y_001642"
     CDS             7847..8524
                     /locus_tag="ACHA6Y_001642"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8987737.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8521..8691
                     /locus_tag="ACHA6Y_001643"
     CDS             8521..8691
                     /locus_tag="ACHA6Y_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987738.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8805..9701
                     /locus_tag="ACHA6Y_001644"
     CDS             8805..9701
                     /locus_tag="ACHA6Y_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8987739.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9756..11126)
                     /locus_tag="ACHA6Y_001645"
     CDS             complement(9756..11126)
                     /locus_tag="ACHA6Y_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8987740.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
BASE COUNT         3593 a         2212 c         2204 g         3278 t
ORIGIN      
        1 ttcgtaaaac aaccgtaacg gttgtgtaaa aatatcattt tagagcataa aaaaaaggag
       61 tggatttctc cactccaaaa aagtctaact tttgggggtc actacagtgt cacagtcctt
      121 tttttagtaa aaggatacat ttcttccttg tatattccat gcgtgttcgt gagaaagcat
      181 atataattta attaacagat agttaggggg agaaaaaatg attttttcaa ttacaatgaa
      241 actggtgttc gggttgatcg gtttattatt agtggtacgt ttgcttggga agaaatccat
      301 gtcagaaatc acaccgtttg atttagtcta tacgcttgtt ttaggtggga tcttagaaga
      361 atcaacctat gatgacaatg tccatgtagg gcatgtccta tttgccattg cgctttgggc
      421 agtaatgatt tatggtatcg aacgtatcgt ccaaaaaaat gaaaaggtca atcgttgggt
      481 caaaggcgag ccatctgttc taatcaaaga cggcgtcatc aacatgacag agttgacaaa
      541 taaccatatc gagatggagc agttgcgagc aatcttaaga cagcaagaat gcttttttct
      601 tgaaaatgcc aaacatgtta tcctagagaa tgccggtcaa atgagtgtat tgaaaaaatc
      661 agatgaagac aaagctttat cgatcctgct tgtagatgaa gggcaaattc agcataaagt
      721 gcttcagtcc aatcaattga ctgaagcatg gcttatggag aacttgaaga aagaaggcta
      781 tgcagatgta aaacagctga tttatgttga atggtcagaa gaaaaagggc tctacatact
      841 aaataaagaa gacgtcatgc atgaatctta ccgaatagat ggatagatag acagaggttg
      901 tgaaatcagc gccttggctt gttactcaaa actaagcgac tttttcacaa cctctgatat
      961 ctaatatacg ttgttccaga agttgcttct tcgaaaaata agccaaaatt ccacaaaaat
     1021 ttaagaagca attttcgaaa aatcctttct tattttttga agttgatcac ttctgtcaca
     1081 acctctcatg tcacaaacca tttttttgtt tcctttaact caatataccg tacgtcttcc
     1141 cacaatatcg gctgatggtc aatcataaag taatcttcat aggcttcacc aatgaagtgc
     1201 ccttcgacct catccaagag tcttccaaat tcatctttta aattgagctg aatgattact
     1261 gctttacgag ataaaaaggc ttctgtcagt atactatcga tttcttgcaa tgtcatttgt
     1321 tgtttaggaa gaggctgttt gcgtgcttct tcttcatttt tttgataccg ttgactagtt
     1381 cttccatcgc aaaagcagtc ccccatttta atccaaatgg ccgatctcga tactcgttat
     1441 agtcaatgaa tttttttgct gtccgtctca tcttttccac cttctaatcc actcatacca
     1501 cctgcatgcc cgccaacgag agaactacgt gcgatggcgc gtcctccttg caagagacta
     1561 tgcgcatgga tgattgattg aaagccgtat ttttttctaa tcgtatcgac aacaaaatca
     1621 actttcgctt catttatctg cttttcagga tcttcaaaaa ggttaagctg taaacctgtc
     1681 gcgtatacga ggtcggaaca gttcacgcct acacgccgaa tatcctggta gtggtaatgc
     1741 tggtggaaaa tggtcaataa agcatctgca agatctctgc tagcatttgt cgcttcgatt
     1801 ttcatttgct ggtgaaaccc ggtcttgcca tttggatcaa gatagcctaa agagtatcct
     1861 acccataagc tgactacttg tgtttttgct gcagatcgtc tcaagcgagt gccgacttgg
     1921 tcggccattt ctttgataac gatctcgatt tcttctcgtc ttgtataatc tctatctagg
     1981 atttgactat ttccgatact ttttgatttt ggtgtatatt cttgtcccaa aaaacttcga
     2041 tcgatgcccc aagcatgggc ataaagctga gtaccgatga cccctagctt gcttttcaag
     2101 tgataataat ccgcgtgagc taaatcataa acagaatgta tacccatttg tttcagacgt
     2161 tttgctgttc gtttgccgat tccccaaaag tcagtgatat ctgagatttt ccataacttt
     2221 tctggtacat cttcataacg ccattcagca aacatttgct ttccgtcttt tgagccattg
     2281 tctaaggcta gtttagcaag tagcgggtta tcgccaattc ctatagtcgt gtagattccg
     2341 gtctgctcaa acacatcttt atggatcagt ttagccatgt cgtacgcatt tgttttacca
     2401 aagagtgcca tgctatttgt tacatcaagg aagctttcat cgacggaata gacatgatgg
     2461 ttcgcttcgt ctgcatattt tttatagata gaattgattt ctctgttctt ttccatataa
     2521 taggccatcc ttggggcaac aatatataaa tcacttggat aggggaaggg aagatctctg
     2581 gcacggctga tattggtgat gccgtaagct ttttttgctg caggactgga agccaagatt
     2641 aatccgctac cccgtgtttc tgtcgaatcg gacggatagg acatgactac taatttagtt
     2701 gtaaggggat caagatcctt ttcaacgcac tctacggagg cgtaaaaaga tttgcagtca
     2761 atacataaaa tatctcttga aggttctttt tcataaacaa ataccggatt ttgattcgcc
     2821 cacataacaa tcacctcttc aacacaatta gaacacacgt tctctttccg tgtcaataaa
     2881 aaacagcaat tttcaaatac gagcaaaagg ctcacatttg gaaattgctg agaggttatt
     2941 tttatctatt tgtttttagc tgagatcgtt gattttttta tcaaacatga acagcttctg
     3001 gatcgatctt tgttgtccca tttccaatgt gtgcaacata aaattccgga gcatagccga
     3061 caactttcat gtattcatca tagacattgt ttttgaaggc aggtatattt tcttctttga
     3121 caagagcaat cgcacagcca ccaaatccag ctcctgtcat tcgtgcacct aagacacctt
     3181 cttgtttttg agcagcatct actagtgtat ctaattcgat cccagttact tcataatcat
     3241 agcgaagaga gtggtgagaa tcatttaaca gttgaccaaa tgcttctaag ttcccagctt
     3301 ctaattcagc ttttgccttc aaagtccgct gattttcaga aacggcatgg cgagctctgc
     3361 gtatcaatgt atcatcgccg atttggtcaa tagttgattc aaattcttct tcatctaagt
     3421 ctcctaaaga agagataggc aaagtttttt gcaaacgtgc caaggcttct tcgcattcgc
     3481 tacggcgttc attgtatttt gaatctgcca attcccgccg cttattcgta ttcatgatga
     3541 cgattgcata accatctagt ttcactggaa ccatttcgta tttcagcgta tttgtatcta
     3601 ataagattgc atgatctagc tcaccgaagc cgatggcaaa ctgatccatg atacctgaat
     3661 tgacaccgat gaattcattt tctacttttt tacctgtttg gactagttcc aagcgatctg
     3721 tttttaaatc aaacagatct tctaatacaa cacctactaa taattctaat gaagcgctac
     3781 tggaaagtcc tgcaccatta gggattgttc cttccactaa taactcaaac ccatgatcga
     3841 ttgagtgtcc agcttctttt aatttaagga ccattccttt tacgtaattt gcccagtttg
     3901 cttttttatc atattctagc tcatctaaag taaaactaat gacaccgata tcgtcaaagt
     3961 ttgtggaata aactaatatt ttattatcgt ctcgaggtgc tgctacacct tgcgttccat
     4021 aagtgatgga agcggggaag acatggcctc cattgtaatc tgtgtgttca ccaatcaaat
     4081 tgatgcgtcc tggtgagaag tattgtgtag tttttttagg tccgaatgtt tcggcaaatt
     4141 ttgtgtttaa gcttcttttg atttccatga tgagttattc ctttcttgtt ttgattttga
     4201 attctgttac tgtttcaaat actgatccgg cttttaaatg aatcgaacca aatgctgaga
     4261 agcgctctgc acctggagct gtttgagtct caaatgtgat gccaccgtga tgtgccagtt
     4321 ttcggttgcg catttctggt gtttctgtgc cgaaatttgc agtgaaaata accacactag
     4381 aagcatctgt agcaatatct acttggattc ttttatcggg gctagtcaag cgagcggctt
     4441 ctttacccaa gccggtttct tttaagaaaa atggatgatc gattccgtca acaagatttt
     4501 tttgatccaa atcactagca aaaacggacg ctaatttttt aggtatttga aaatcgaaag
     4561 cacttcctgc agcattttct tttaccccta taggaatact atctgttcgc aaaggagcat
     4621 aagcttcgct attgagccaa agctcatgtt gatcgattga ctgagaagca tctcccgtca
     4681 agttgaagta aacatgattt gtcggattga ataaagtatc ttggtcactg gtgccgcgag
     4741 aggtcactcg ccagatatta tcttttgtca aagtatagcg gatttccaca tccattgtac
     4801 ctggaaaacc atgttctccg tcgggacttg tagtagtaaa aatgacagaa gcttcatttt
     4861 cgccattcaa aatgacataa gaccattttt ttaattcaaa tccaggagca ccaccatgca
     4921 gattatgtcc agtttttgga tctgtggcta attggtaagt ttttccattg agagaaaagc
     4981 ggccattttc gattctccct gcggtgcgac cgatagaagc accgatatac ggatcttttt
     5041 cgatatattc ttcagcggtg tcaaatccca aaactaattc tcgatcattt gattttaaac
     5101 tgacgattct agcacctaaa tcagagatag aaagtgctag cttgtttttg ttctccaagg
     5161 ttatcaagtg ataaccttgg ccaaaatctt tttctgtaat cttcattttc aagatccttt
     5221 cttaatggga aggaactact gcatcgtgaa gttaatcttc cattgtatca gctggagcca
     5281 atttgttttc caatttctca acgatttgtg catgtttttt ctctgtcaat gtaatcttcc
     5341 aagcaaaaat aatagcggag aaaatcatca aagcagcagg agcatagaat gcataagctt
     5401 tgaaagtcgt tacgccatga gcagtgatat cagcagcagt agcattacca gtcatcccag
     5461 aagcgatagc gacaaaccct acgatcccgt tagacaaagc accagcgatt ttatctagta
     5521 aagggcgaat tgatagagta acagcttcgt tacggatacc atttttccat tgaccatatt
     5581 caacagagtc tgtgatcgtc atcaaagcgg ataagaaaat caattgttga gggaagtaga
     5641 acaatactag cccgatgata acgattggca aacttgaacc tgcgatcgta aagaaaaagt
     5701 aagaagaaat catcattgcg atccctccaa ggtaaacgta gcgacgagag atgacgcgtg
     5761 taagcattgg gaatagtgga acagctacta aaccaagtaa ggcagcaact agaccaacga
     5821 tccaaaattc agttggttgg ccaagtacat atttgaagaa gtagaataat acggcagttg
     5881 ttgctgcatt ggcaatcgca taagcgatat aagcaagggc aagccacatc aactgatcat
     5941 tttgagcaac tgctttgaac acatctttga tcccagcttt ttcattttgc tgacggatcg
     6001 cagtatcgac ttcttttgtg cccaaggcag tgataatcgc agtaccgcct gaaataacag
     6061 cagcaatcac accgaaccag aaccagcctg aagcgccttc aatatgttta tgagtaacaa
     6121 gataggtaaa gaaagtaacg atagggacaa cgactagagt agtaccattt gcacctagag
     6181 aagagccgaa acgagcaaac gtagcaaatt tttcacgttc ctttgtttcc gtactcaaag
     6241 ctggaatcat tgaccaaaaa gcgatatcct taaatgaata aaaactatcc aataagatga
     6301 aaacgattgc aaataatatg ataaataaag tcgtattatg agtagctaag ccaaagaaat
     6361 tggtaaagat tgctaccaag agtacggaac taacaacgcc gccgataact aaccatggtt
     6421 tgaatttacc gtattttgta cgagtattat cgatgatgcc accaatcaaa ggatcaaaaa
     6481 taatttcaac taagcggata cctacaacta agcttgtaac tgtagcaatc atttttgcat
     6541 tcgttgcctt atctgttcct gcgaacatct ggcttgtcac gaataccata aaataagtgc
     6601 ttaatgtaac atagaatgtg tcgtgtccaa aagcgcctaa agcgtaggac acacgttgga
     6661 tcattttgct tttcataatc tgtattcctc tcctgttcaa tatataatac taagtaaacc
     6721 ggttacaacg aataacaaag atcttttaat gacttaaact taacaagaaa tcgcttcctt
     6781 tttaatatat ttctgtgact tttatttaac tttttgttgc attatactaa aggagtaagg
     6841 agggatagac gtggagcaat cgattttttt aaaaaaggaa catcaacaag tagaaaaaga
     6901 attgtatttt gatttttgtg gtttctctaa aacgatgccg tttcattctt ttggtcctgc
     6961 tattcgcaat gattacatcc tgcatgtcgt gatggaaggc aaaggatctt atcatgtgaa
     7021 aaatcaacaa ttccaattga aaaaaggtga tctgttcttg attcgtccag gagactcgac
     7081 gttttactta tcagatggag aagatccgtg gatgtattgc tggctttcat ttggtgggag
     7141 tgctgcagaa gaaatcattc ggcagtcgct tttcagagaa gaccaataca cgatggtctc
     7201 ttctgacata caaaaatata tcgacattat tttagaatgc atgaaagata cggaagaaac
     7261 cttggaaaat gaattacagt taacagcatt aacctaccgt tttttatcag tccttttaaa
     7321 agatggcgga aaggtcagcg gtggtgtaca aaaggttcat tcctctttag ctgttgaaac
     7381 agtcaaatac atcgaggcac attatgctga aaaattgtct gttgaggaaa tcgcccagca
     7441 tttagcggtc aatcggagcc atttgtcgcg agtattcaaa aatcatatgg gtaccagtat
     7501 caaggaatat ttgatcgggg tacggatcaa tcgaggggcg tttcttcttt ctctgacgga
     7561 tgaatccgtg gaaagtattg cctatcaagt agggttcaat agtctagtcg tatttagccg
     7621 aatgttcaaa aagagtaccg gagaaacggc tacgaactat cgtaaacgta tgaaaaacga
     7681 aaattttaag aatattgctt tggatacctt gaaaaaacag ttggaaaaac aaaccgcagt
     7741 ttcttggaca acctgattag tctggttaaa atgacaagca tcggcaaaag aaacttgtgg
     7801 cggttatgct acactgtaaa gcgaagacta aaaaggagga aaagggatgc gtacacttat
     7861 catcattagc catccaacga tcgaagaatc tttaaatcag caatttttca aagaagcttc
     7921 tgctggttta ccagctactt ggcatcattt ggaatcaagt tatccagacg gaaaaataga
     7981 tatagagact gaactacagc tcttggccca gcatgatcgg atcattttcc agtttccgtt
     8041 ttactggtat agttctcctg ctcatttaaa aatttggcaa gataatgtct tggaacgagc
     8101 agcacatgta ttaaaaggga aagaactagg aatcgtattg acaacaggtg tggcggaaaa
     8161 agaataccaa gctggaggaa aagaagaata tacgatttcc gaatttttga ggccgtatca
     8221 gcgaatcgca aataaattcc atatgaccta tcttccgcca ttcgttttag cacaatttat
     8281 gtatttatcc caggaaaaac gttgggagaa gctaattgct tatcagcagt atctctcatt
     8341 ggaaggcaaa ccatcgctga ctcagcggat tgactggttt attcaaagag tacaggaaaa
     8401 tcagaaaatg caggaagaag aatctgaaaa acaagcatat atcgtagaag cactgactga
     8461 ggcaaaagag cagatcgaag acttgacctt tacactgcaa gaaatgaaag gaacttcatt
     8521 atgacaaatg attggctgat ccaacaatta gatgaattaa gcgaaaaatc agaatatcaa
     8581 gaccgtgcgc tcttatatga aataaagaaa ctgatggctg agatagacga acgaacaatg
     8641 cagctgcaag gagaaatcga cggacaatct tggaatcatc tcaactggtg aaattttctt
     8701 ataaataaca aagataaaaa tgtgcacagc gacaattatt tgctacaata agaatatgaa
     8761 gcgtttgata acgctattat acataaaaat tggtggtgaa aaaaatgtat gatgaaaatg
     8821 agtggggaga aaatgttcgg atgacccgaa gaagacagca aaaacaggca gctcgtaagc
     8881 ggatgatcat gattggggca gcagttgccg tgtttttgat catagcagga ggagttgcgg
     8941 gtctatattt tcatggaaaa aattcgtcag aagcgaaaag tacgaccata acggaaacaa
     9001 cgacaaagaa aacagagacg aaacaaacaa gtgagtcatc aactaaaaca aaagagacac
     9061 aaacttctga agaaaaaaca gcaaatggtt tgccagagca acttcaagca atcgtaaatg
     9121 attattcgaa aaagatcgaa gaaaagacgc ctgcattgat tgaagaatac cagacagaaa
     9181 ttcagggaaa tcaagaggga atagctggtt tatcagcagt tgctaaccaa aaagcacgtg
     9241 agcttcaagc aatctccgac gaagggatca gaaaattacg tgcagcgtat caggcagcag
     9301 aaaataaaga cggtgtagac ttagatactt tgatcaatca attatcggct aattatacga
     9361 atcatgtcgc taagatttcc gatatttatt tgcagactag cgcctctctt caagcagagt
     9421 caacaagcac acaagacaca acatcttctg attctgagac agcagagtcg gtagaagctt
     9481 ctgaaatgca agctcgaaca acacaagaca caacagattc ctctgaaact aataccaaca
     9541 gcgcgacttc acaggctgcg acaaccgtag ttttggaagg agaaggtcca aatcaaatag
     9601 ccgaacgaac tggcgtacca gtagaaacga tcttgtcgct gaatgggatg agcatggaga
     9661 attatttcct gactcccggt gagacgctaa aattgaattg ataaaagcaa taaaaaagaa
     9721 cgaagcttcc actaaggtga agcttcgttc tttttttatt ttccggttct tttttcgcgt
     9781 acaaagtaat ggatcgtcat gtaaagcaag aaaacgagtc caataatcaa tgagattctc
     9841 gtttcaggat tgataaacat gaaaatcaat acgatagcta aaaaagcaag tgtcacataa
     9901 ttcgtataag gagatagtgg cattttaaaa ggatgatcat gcattttatc tgcgtgcttc
     9961 tttttgaagc gaatctcgct gatcaaaatc actacccaag gaaccatacc aggcaaaata
    10021 cttgagctgt atacgaaaac gaaaatattt tctgactggt tgatgaacat tggtagaaga
    10081 taatttaaga tcagaccaat taagattcca cctgagattg ctacgacagg ataaaaaggc
    10141 gcaccgtgac gagatagttt cagaaatttt ttagacatct ttccatttat ggcaagtgta
    10201 tagaccatac gacttgcgct gaaaatccct gaattgcaac ctgataatgc cgctgtaatc
    10261 acgacaaaat taatcagtcc tgcagcaaat gtaatgccga ctttagaaaa agtttgtaca
    10321 aaaggtgaac caatctcact caattgattc cacggataga tactcacaat gataaaaata
    10381 gcaccaatat aaaaaatcaa gattcgaccg atagtggact ggacagcttc aatgatcgtc
    10441 tttttaggat tctctgcttc accagcagtc attccgatca gttcgattcc ttgataggaa
    10501 gcaacaacga tcgataatgc aaagaaaaat cctttgacgc ctccagtgaa gaatccacca
    10561 tttttccaaa gatttgaaat ccctactgcc tgaccatgat taccaaatcc aaataagatt
    10621 accccaagac ctgcaatgat catcaaaatg atcgttatga ctttgatgat cgaaaaccaa
    10681 aattctaatt cgccaaacgc cttgacagaa ataagatttg ccagcataag aaacaagacg
    10741 acaatgattc cgggaataat tgccggcaaa tcaggccacc aataattcat ataagatcct
    10801 acagcaatca cttcacttat tcctacgaca atatattgaa aaatattact ccaagcggtc
    10861 aagtagccag ccacaggatg gatgtactcg ctcgcgtaat ttgcaaaaga accagtcgaa
    10921 ggatcaacgt aaagcatttc gcctaaagca cgcataatca aatacaaaaa gaatcctgca
    10981 atcgcatagg ctcccatcac tgagggccct gtccaacgta tggttgagga ggcacccata
    11041 aacagaccga caccgatcgt tccacctagt gcgatcattt ggatatgacg ggcactaaga
    11101 cttcgagtta acttttcttc ttccatacaa aaactccttt cgttgttgag tcaacaagaa
    11161 aaaataatca aacgtttcaa cacatgtttt tcattattag gttagcctta ttgaactctt
    11221 gatgtgattc tacagaaaat tttcataaaa tgcgaggaaa attcgaaaaa aactttttca
    11281 tcattct
//
LOCUS       DAYYHX010000086        11181 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000086 DAYYHX010000000
VERSION     DAYYHX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.087"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(416..1684)
                     /locus_tag="ACHA6Y_001646"
     CDS             complement(416..1684)
                     /locus_tag="ACHA6Y_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8987741.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(1915..2652)
                     /locus_tag="ACHA6Y_001647"
     CDS             complement(1915..2652)
                     /locus_tag="ACHA6Y_001647"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8987742.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(2719..3288)
                     /locus_tag="ACHA6Y_001648"
     CDS             complement(2719..3288)
                     /locus_tag="ACHA6Y_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8987743.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            3416..4288
                     /locus_tag="ACHA6Y_001649"
     CDS             3416..4288
                     /locus_tag="ACHA6Y_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8987744.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4630..5502
                     /locus_tag="ACHA6Y_001650"
     CDS             4630..5502
                     /locus_tag="ACHA6Y_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8987745.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            5499..7457
                     /gene="nagE"
                     /locus_tag="ACHA6Y_001651"
     CDS             5499..7457
                     /gene="nagE"
                     /locus_tag="ACHA6Y_001651"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8987746.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(7708..8559)
                     /locus_tag="ACHA6Y_001652"
     CDS             complement(7708..8559)
                     /locus_tag="ACHA6Y_001652"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8987747.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            8682..9149
                     /locus_tag="ACHA6Y_001653"
     CDS             8682..9149
                     /locus_tag="ACHA6Y_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM8987748.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(9412..9894)
                     /locus_tag="ACHA6Y_001654"
     CDS             complement(9412..9894)
                     /locus_tag="ACHA6Y_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8987749.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(9994..10857)
                     /locus_tag="ACHA6Y_001655"
     CDS             complement(9994..10857)
                     /locus_tag="ACHA6Y_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8987750.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         3289 a         2280 c         2053 g         3559 t
ORIGIN      
        1 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
       61 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
      121 ttgattctta tcaaactata gaagatagct cttctgcagt agaaaatttt acaccgccaa
      181 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
      241 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
      301 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
      361 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
      421 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
      481 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
      541 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
      601 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
      661 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
      721 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
      781 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
      841 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
      901 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
      961 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
     1021 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
     1081 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
     1141 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
     1201 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
     1261 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
     1321 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
     1381 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
     1441 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
     1501 ttgtattttc tgagacagta gctgactgca aagtactctc tgtatcgttt tcaattcctg
     1561 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
     1621 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
     1681 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
     1741 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttgata aatttgcttt
     1801 ttctacataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
     1861 tcgtttgaat aactatttaa caagtaattc tctttggata ttatccgtta tggattatac
     1921 ttcctgtgtc gtcggacgga taatcatgtt actgacagac atccggtctg gcgtttctat
     1981 cgcaaatgct acagcagaag ctatatcttc ggaggtcaat ccccattctt tttgcgcttc
     2041 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
     2101 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
     2161 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
     2221 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
     2281 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
     2341 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
     2401 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
     2461 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
     2521 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
     2581 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
     2641 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
     2701 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
     2761 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
     2821 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
     2881 tttcttgatg tggattggct ttactaacaa gcctcttcgt tcttttggat cggttagatg
     2941 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
     3001 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
     3061 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
     3121 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
     3181 tgctaaaggc tcttcgtttt cagcttggat ctgatggatc acttcaaaga gcggatccgt
     3241 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
     3301 ttttatcctt actttataac ggacggctta tcatgtctaa tacttataaa acatacttaa
     3361 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
     3421 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
     3481 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
     3541 acttggtgtg acacttttca ctcggggctc ccgagagata cagttgacgg attctggtcg
     3601 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
     3661 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
     3721 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
     3781 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
     3841 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
     3901 agatcgttgg ggacttttga tacatcgaaa tcatccttta gcaaagcaaa ccgtcatcca
     3961 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
     4021 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
     4081 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
     4141 gatcatcaat acttatggga cggaaaaaat attcatccca ctcgatccgc cattacaagc
     4201 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
     4261 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
     4321 aacaacgaat aagataagca aaagatccaa cgattaaata acataagttt cttaatttat
     4381 ttcttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
     4441 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
     4501 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
     4561 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
     4621 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
     4681 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
     4741 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
     4801 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
     4861 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
     4921 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
     4981 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
     5041 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
     5101 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
     5161 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
     5221 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
     5281 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
     5341 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
     5401 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
     5461 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
     5521 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
     5581 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
     5641 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
     5701 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
     5761 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
     5821 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
     5881 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
     5941 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
     6001 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
     6061 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
     6121 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
     6181 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
     6241 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
     6301 acaaagcaaa aacagcttcc ttgatgctag cagcagcttt tgcttcattc tttactggtg
     6361 tgacagaacc acttgaattt tcatttatgt tcgttgcttg gccattatat gtcttgcatg
     6421 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
     6481 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
     6541 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
     6601 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
     6661 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
     6721 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
     6781 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
     6841 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
     6901 ttgcagatga gatggcaaaa ctacatggtg gtgctgcagc acgacctgca caaacaaaca
     6961 ccgtggttaa aacagaaaca tttgccggtg aaacgatcga acaagatatt tacgctatcg
     7021 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
     7081 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
     7141 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
     7201 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
     7261 atgtagaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
     7321 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
     7381 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
     7441 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
     7501 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
     7561 actccgcggt aataagtcca gctaaacgaa acatatgaag agctattttt tatttgactc
     7621 ttcttattac ttgatgcaga acagcttttt cacaaaattt ttcttcatac tcaaaactaa
     7681 tcggcttttt cacaacctct ttatacatta ttcctgatcc aatatttctt ttaatgctgc
     7741 ttcaagagaa gttaatggtt ttcccaatac tttttctaaa tcatcagagg aaatagctaa
     7801 ctgatccttt ttaatatcat gttggatcgc taaaaacatg tcagctgctt cttgagggaa
     7861 tccggcttct tttagatttt cgacaaacgc tgcatcactt gcttcaataa tttctaactc
     7921 tttacctgtc gcacgctcta atgccttggc taattcttca tagtcagtta gattgccgga
     7981 taattctaag atttctggag agtcactttc tactagtact tttgctgcag cttctgcata
     8041 ttcacgtttc aatgcccaac ctgtttttcc atttcctgca gcataaacga attgaccagt
     8101 ttttaaagcc tgcccaatga taggtaattc attctctaca taccaattat tccgtaaaaa
     8161 tgtatgggaa atacctgaat ccttaatcat tttttcagtg aattggtgat ctggcgctaa
     8221 tacactgatc gagtgatcgg catctgcaaa acttgtataa gcgatataag ataccccagc
     8281 tgcttgtgct gccttcacta cattcttatg ttcggcttga cgatttcctg gaacgccaga
     8341 gacaaaaagc aatcgatcta ttcctttcaa cgcttctttc attgagtcta aatcatcata
     8401 atcaccaata cgtatattaa atccagcttc tttcagtgct tctccttttt cttcactacg
     8461 tactagtaca tagatttcat tttgtggaac catctttttt aagaattcca gcgcataacc
     8521 accaaaacct ccagttgctc ctgttactaa ataatgcata tttgccactc cctattcttt
     8581 ttatttgtta agatttacag ctgtacattt ataaattaca cctttatcat aaagagtgtg
     8641 gtaagataag tcaagcaaaa aaaatgaaag gatgacaact gatgaagtat tctctgcaat
     8701 ttagtgatgc aatccatgta cttgcctata ttgaaatgtt tcaagatacc cgatcacttt
     8761 ctagtgagat gattgccagt agtatcgaaa caaatgcagc aaatgtacgt aaaatcatga
     8821 gtcagctcaa aaaatccggt ttgatcttta caaaaaccgg aaaaccgcag ccttcccttg
     8881 ccaaaagtcc agatcagatt tctttactag atgtctatcg cagcatcgaa ggtaatacac
     8941 agcttatcca agtcgatcca aaaacaaatc caaattgcgt cgttggtgcg aatatccaag
     9001 aagctttaga agaaagctac gctcggctgc agaaaaaggc agaagaagaa atggctaaga
     9061 tcacacttga tagtctgata cataaaatag ccgttttgga aaaagaaaaa agaccagaaa
     9121 acacaggcct cgtagagaga tttttataat tctctctact gtgcctgcat ttttctgatc
     9181 ggaatcgtct acataaattc ttttattctc cgggggacaa aagccttgtc atgccccttt
     9241 ttcctctcta atgctcaggt caaagcgctg aagtcatgac gtcttgataa acggtgttca
     9301 agtagctgac cctcggcatt agttctgaaa aatgcaaaat atggaaagct atttcccatt
     9361 tttcctctct aatgctcagg tcaaagcgct actttcacga cctctatatt atcatggttt
     9421 attgactacc ggtatccaga tttctcccat atatttcccg ttggtaactc ccatttcgac
     9481 tacagcattt ggacccccaa cataggcgta atcttctact tctcttaaaa catgcccaaa
     9541 ggccgtattc gtcaaatcat ttcttaattc ttcaggcgat tctgctaaag cgcttacaac
     9601 aagatacgtt ccttttggga atgggatgat tcgcgtagtg tttggaacat cctgactcgt
     9661 ttgaacaccg acatagtaca tcattttccc gttatagggt tcgttgactg taaagaaata
     9721 atgattttca gcagcttgtt tcaatgcatc tattgttcca ttaccgataa ctttttgctc
     9781 aaatgcttgt ttctctgcag ccaaaccgac aaaatccgta ttgctactct tcaattccac
     9841 accaataccc gtcaccgtaa aatctggttt ttcttcgatc ctatactttg ccatttctcc
     9901 caccctttct attatattaa gttcatctta cataaaatta aagggaaatg acacgattac
     9961 gctctttttt ctagggatat ttttattttt taactatttt tttgctttca ctgcaatcca
    10021 aatctcactg taaacattac tgaaatcctc tccccatttt gtaaaagaga tctcgggagc
    10081 ttcgactaac ccataatctg aagaaggaag ccattctgca tagattttcc cccaagtatc
    10141 ttgtaaagtt tggggaaatg gccctttatt aggaaaaatg gcccaagtgt gttcttctat
    10201 agcaagcatt tctaaatcag cgtacggatt ttcttttgtc gtagcaaagc caatcatgtg
    10261 catcaaactg cccttttctt ccaagcggtc tccatcaaat ccataagaaa cgttgattac
    10321 ttgatttgga tataaatctc ctaacttatg catctcttcc ctttgagtct ccgtgatcgt
    10381 ctgcgctaac ttttgtattt cttgattgac tccttcaaat ttgacaggaa cttttttcgc
    10441 cacacctgcc acataaaact gttctttctt ttcgatacga aattccatcg attttcctcc
    10501 tctgacatca ataaaaaatg tcaagcgtgg aaaagatttt tgtatgccgt atctgctgat
    10561 ttctgaaggc agatatcctg accaatcacg aaaagctctt gaaaaacctt cgactgtttc
    10621 atatccatat ctaaacgccg tttctgtcac tcttgatcca tctagaagat cgcgattagc
    10681 caaagccaat ctacggtttt tgatatagtc cattagcgac atccctgcaa taaacgtaaa
    10741 gatgcgtttt aattgatatt cagagattcc tgtattttcg gctattattt tataggaaag
    10801 tccttctgtc aaatgttctt cgatgtaatc gatcaattgg ttcagatctt taagcatatt
    10861 tatcttcctc cttgattgcc tttttatcat attatcaaag aaagtcaatt acttgatgaa
    10921 tcaggaacca atttagtcag atgtagaaaa tgttgtatgg atctaaagaa aattctcaag
    10981 cagcgtttcg taacggataa ttggtattat ctgcctatct atgttttacc tttagaaaaa
    11041 taaattttgg ggaagacaga ttctaagtaa acaaagcggt aaaaatatct tttattccct
    11101 tagttaatcc gcttactacc tactgtacaa agcaaattgt tgttcaaact gctgatcaga
    11161 acgctgcttg gatttttctt t
//
LOCUS       DAYYHX010000087        11011 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000087 DAYYHX010000000
VERSION     DAYYHX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.088"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..1355
                     /gene="efmA"
                     /locus_tag="ACHA6Y_001656"
     CDS             69..1355
                     /gene="efmA"
                     /locus_tag="ACHA6Y_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM8987751.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1493..2092)
                     /locus_tag="ACHA6Y_001657"
     CDS             complement(1493..2092)
                     /locus_tag="ACHA6Y_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8987752.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2496..3617
                     /gene="mnmA"
                     /locus_tag="ACHA6Y_001658"
     CDS             2496..3617
                     /gene="mnmA"
                     /locus_tag="ACHA6Y_001658"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM8987753.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3863..5284
                     /gene="arcD"
                     /locus_tag="ACHA6Y_001659"
     CDS             3863..5284
                     /gene="arcD"
                     /locus_tag="ACHA6Y_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM8987754.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5298..6416
                     /locus_tag="ACHA6Y_001660"
     CDS             5298..6416
                     /locus_tag="ACHA6Y_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM8987755.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6476..7768)
                     /locus_tag="ACHA6Y_001661"
     CDS             complement(6476..7768)
                     /locus_tag="ACHA6Y_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8987756.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            7918..8478
                     /locus_tag="ACHA6Y_001662"
     CDS             7918..8478
                     /locus_tag="ACHA6Y_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8987757.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8607..8984
                     /locus_tag="ACHA6Y_001663"
     CDS             8607..8984
                     /locus_tag="ACHA6Y_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM8987758.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            8917..10989
                     /locus_tag="ACHA6Y_001664"
     CDS             8917..10989
                     /locus_tag="ACHA6Y_001664"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8987759.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         3547 a         1984 c         2387 g         3093 t
ORIGIN      
        1 aaaaagaatc ccgtataatg attgttgtgc aaacgctata caaaatgaaa aagaaaagag
       61 ggatgtttat ggaaaatgaa cagtcggtcg ttttaacgaa ttggaagaga aattatttgt
      121 tctttttatc gggacagttt ttgtcaggaa tcactagtat ggtcgtccaa tatgcgatca
      181 tctggtattt gacaagagaa accggttcgg cgacaatttt gagttttgct actttactag
      241 gaatgattcc catggtttta ctaagcccat ttgtaggacc gctagtggat cgctgggata
      301 aaaaagctct tttgattgtc acggatatta ttgtagcgat tttcgcgctt attttagcag
      361 tagtcggaac gatttcagaa tccttcccga tttggctagt ttttgtgtct ttgtttatgc
      421 gttcggtcgc acaaacattc caaatgccaa cgatccagtc gattatgccg acaatcgttc
      481 catcttctca cataacaagg acgaatggac agttaggaat ggtccaatca gcgaatttca
      541 tcattgcgcc agctctaggt gcggctttgt tttcagtcgt tcctgtcaat taccttattt
      601 tattagatgt attgggggca gtttttggtg tcggcttatt gatttttgtg aagatcccaa
      661 aagtttctcc tgaaatattg gaagttccgc ttactatttt taaagatgca aaatttgggc
      721 tgcagcaatt gatggataat aaagggttat ggtacataac gatcaatggt gcatttgtga
      781 tgctgttgtt catgccagca atcagtctgt atccattgat gacgctagat tactttggtg
      841 gttccgttgg acaggcggga gcagtggaag tagtctacgc agtcggaatg ctcttaggcg
      901 gtgcactgat cagttttata ggaacatgga aagaccgaat gaaacctatt attatagcct
      961 acatcattat gggcctgacg atcggtgcaa gcggattggt tccaaacgat agtcaagggt
     1021 tcttgtactt tcttatcttg aatgcaggag caggttgtgc aacgccatat ttcaatacac
     1081 tgctgatggc aatgatccag cagagttatg aatcgaatgt tttaggtcgt gtcttaggga
     1141 acttcaactc tttgatgaac ctggcaggtc caattggatt attatttgca ggaccattag
     1201 ctgatcgttt aggggtagaa aaaatgttct tgttttccgg aatcgggatt ttattatgcg
     1261 gaatcgtttt atttttaacg tcagctgccc gaaattatga taaagaatta cagaaaaaac
     1321 tggtaaaaga acaccatgag caaaaagacg aatagtatgg aatggagttg tgacagaagt
     1381 cttgttacaa ctccatttct gatcctaatg ttcctgtggc aataagccta ctcaaatgaa
     1441 aaatatgagt aattatcttt atttgtttaa tacttgatat agggcagttt tttcacgacc
     1501 tatcgtactt cctctttgct tcatagattt ctttgatttg ttgctcagat aacggagtgc
     1561 caaatggtag tttttccttt aagagttctc tttccggtgc gtctacgcct acattgacat
     1621 cgttctcgat aggtacgctg taatgatgga tatgtccatg aagattgcgg atagtgtcgt
     1681 taggcccgag caacatcgga taatgagtca agtaatattg ttgatggtcg aatttaacga
     1741 tggcacctac atcataaaaa taaaatttgg gacttccgtc agtaagccta ggatcatttt
     1801 tttctaaata acggaaaaaa gcgcgactgt catggttccc tttgatcaag tggattgttc
     1861 cttgcagttg agtgaccagt tccaaaattt ctgcatttgc tttttcatat tgcggatgca
     1921 tagcaaggtc gcctagatga taaacatggt cggtttcttt tattactgca ttccagctag
     1981 tgatcaactg ttggttcatt tcatccacat ttgcaaagag ccgcggagca aagtcgtttt
     2041 ttcctaataa gcgttcatgg aaaaagtgca tatcactgat aaagtaattc atgtatcttt
     2101 tgcctccctt ttttcttaga gtaaacaaaa tcacttatct gagcaactca taacatctaa
     2161 aaagaaaagg ttgtaaatga gacgtgcagc tttaagcgat aagaagcaat tttgaaaaat
     2221 agtttttcac aatttataaa attattcctt attgccgaaa ggctggccct ttttatgagt
     2281 atgccaattt tttatatcga atgctcaaaa gatgagtgat tttttggttg actatcatgt
     2341 ttgtgtactg aataggtcca gtagaacagt atttttacga cttgataatt aagggatgaa
     2401 attttcagaa atatgacgta taatgtaaaa tactgaaaag ttgcgacctt caactcgtag
     2461 atgtttcaga atctaattga aatgatggtg atatcatgac agacaacagc aaaacacgcg
     2521 ttgtcgtagg gatgagcggc ggtgtcgact catctgtaac agcacttttg ttaaaggaac
     2581 aaggatacga cgtgatcgga atattcatga aaaattggga tgacacggac gaaaacggtg
     2641 tatgcacggc cacggaagat tataaggatg tagcgaaagt agctgctcaa atcgggattc
     2701 cttattactc tgtgaatttt gaaaaagaat attgggatcg tgtatttgaa tatttcctag
     2761 ccgaataccg cgcaggacgt acacctaacc cagacgtgat gtgcaacaaa gaaatcaaat
     2821 tcaaagcatt cttggattat gcaatggatc ttggcgccga ttatgtagct actggacatt
     2881 atgcgcaagt cactcgcgat gaaaacggcg tggtccacat gcttcgtgga gtagacaata
     2941 ataaagatca aacttacttc cttagccaat tatcacaaga acagctttca aaaacgatgt
     3001 tccctctagg cggcatggaa aaatcagaag tacgggcaat tgctgaacgc gcaggccttg
     3061 cgactgctaa gaaaaaagat tcaacgggaa tctgctttat tggtgaaaag aacttcaaac
     3121 aatttttgag caactatctg ccagcaaaaa aaggaaatat ggtcactctt gatggtgaag
     3181 tcaaaggaca acatgctggc ttgatgtatt atacgattgg tcaacgtcaa ggactaggga
     3241 tcggtggcgg aggagattcg caagaaccct ggtttgttgt aggaaaagat ctagcgacaa
     3301 acacattata tgtggggcaa gggttccatc atcctgcatt gtatgccaca agcttggatg
     3361 caagtgaaat ccactttacg acgaatgaac caatgccaaa agaattcaag tgtacagcga
     3421 aattccgtta tcgtcagcaa gacgttcctg tgacagttcg cttattggat gataatcgtg
     3481 cagaagtcgt attcgacgaa cctgtacgtg caatcactcc tggacaagca gttgtatttt
     3541 atgatggaat ggaatgtctg ggtggtggtt tgatcgacca cgcatatcaa gaaacaaaag
     3601 ttttacaata cgtttaagcg atattaaatg aatcaaaaca gctatggcta acaaaagaaa
     3661 caccttttct tttggatagc atagctgttt ttttgcaata aaattaattt ttattcattt
     3721 tatacttgaa atacttgtgt aaaaagttca taatatccaa taatatgaat aaagttgctt
     3781 tttgttaagc aagagcagtt gcaaaataga agggaatctt ttgcaagtga tttgttcaat
     3841 tttataagtg agggtgatgg aaatggaaaa gaaatcgacg ggtattagca aatggggact
     3901 ggttgctctg gttgtcagtt cgtcaatcgg aagcggtgtg tttgggatca caagtgatct
     3961 ggctagttct gcagctccag ggccagcgat cctttcatgg ataatcgttg ggattggtat
     4021 tttagctttg gtattatcgc tgaatcattt aggagaaaag cgtccagatc tagatggtgg
     4081 tattttcggt tacgcggaag cttcatttgg taaattaggt ggcttcatca gtggttgggg
     4141 ttactggttg tctgcgtggc tgggaaatgt tgcttttgca acgatgctga tgagcgccat
     4201 gggagaattt ttcccgatat tcaaaggtgg acaaaatgtt ccttctattt tatttgcaag
     4261 tatttttatt tggggtctga ctttattagt aaacaatgga gttgaaggag cgagcgtcat
     4321 caatacaatc gtgacgatct gtaaattagt gccattattc ctatttttaa tatttgtatt
     4381 tatcgcattt aaagtgaata tattcactgc tgatttttgg ggtaacgtat ctgataatct
     4441 agtgaatgga acagggcagc aaggaacgat ctggttgcag attaaaggtt gtttgatggt
     4501 tatgatgtgg gtctttgtag ggatcgaagg cgcaagtgtt ttagccaacc gcgcgaagaa
     4561 acgttcagaa gcccagcaag catcgatcat tggtctgctt tgtttattgt ttatctatat
     4621 ccttgcttcg atcttgccat acggcgtctt atctcaagaa gagttagcaa ctatttcaca
     4681 acctgctatg gcaaatattt taaaaggaat cgtgggaaat tggggagcag cgctgatcaa
     4741 tatcggcttg attatttcga tcattggttc ttggttgtct tggacgatgc tcccagcaga
     4801 aacaacgatg ttgatggctc gagacggtct gttgccgaaa ggttggggaa aaacgaataa
     4861 aaaaaatgcc ccaacttatt cgttagttgt tactgcaatc ttgactaatc tattcttatt
     4921 gactttctta gttactgatt atgcctatca atttgcctat tctttatgta cagcggcaat
     4981 tttgatttgt tatctacttg tcgggatcta tcaagtccag ttttctttaa aagaaggaat
     5041 caaaaaacaa gtagtgatcg gtgggattgc tgtagttttt gaattgatgg gcatcatttt
     5101 agcaggattt tcttatgtcc ttttatgtag tattgcctat cttcctggtt tttgcttcta
     5161 ctggatcgct tgtcgtgaaa ccaaacatca aattacggga aaagaaaaag taatgattgg
     5221 gctgatttgt gtggccgcac tgctttcaat cgggttgctt gctgtaggct ggatcaagat
     5281 ataaggggga aagaaacatg aaaatcgcaa atttcggtgt agaagaatgg ctaaataaat
     5341 gggaaaagaa agcggtatat gatattgcac aaagctcgat cgaagcattg acattagacg
     5401 aactcgttgg cttagatggg actagcgtaa gtaaattttt cgaaaaacgc aaaaacgaac
     5461 cattggacta tggctggatc gaaggatcag atgttttcaa acaagaagtc gcttctcttt
     5521 atcaaaccgt cgatccggaa aacatcttac aaacgaatgg agcgactggt gctaatcttt
     5581 tagctctata cgcgttagtg gaagcaggag accatgttgt ttcgatgctt cctacttatc
     5641 agcaattata tgacatcccc aaatctttag gtgccgctgt tgactttgtt catttgaagg
     5701 aagaagaaga ttggcagttt gatttagaac agctagaagc cttagtcaaa cctgaaacaa
     5761 aaatcatctg tttgaatagt gcaaacaatc ctacaggcac attgctagat cggaaaactt
     5821 tagaaaaaat cgcagaaatt gctcgtacag tagatgcata tgtattgata gatgaagtat
     5881 atgcgccttt aacggataaa ggtgaattct tgtcgatcgt agatgtctat gaaaaaggta
     5941 tagcaaccaa ttccctatcc aaaacctact ccattccagg tattcgtatt ggctggacag
     6001 ctactggacc tgagttggca gaagtttttc gaaagtatcg agactacacg atgatttgcg
     6061 gcggagtcct gtcagatgat cttgctgtac atgcattgaa gaataaagaa aaaatattgg
     6121 cgcgaaacaa aaagatcatt acagagaacc ttgtgatttt aaaacaatgg gtagccaatg
     6181 aaccgaaagt tgaacttgtc gctcccaatt acgtctctac ttctttcatt aaattagcca
     6241 tcgaagaaga tgaccagaca ttttgtatca atttattaga agaaacagga gtactattag
     6301 taccaggaag tgcctttgat ttaccgaagc atgcccgact aggttactgt tgtaaaaaag
     6361 aaaccttgga aaagggatta ttcctcttat ctgagttctt gaaaaaacag gcctaaacaa
     6421 aaacactgca aggacagtct gtgacaactg ttcaagcagt gtttttcatt tacgcttatt
     6481 gtctatgaga aggagagtgc cctgaagcta tggagactcc tgaagctttt tcttctgcag
     6541 tttcttgctg atggatctct tgtgtattgt gtcgtacaag caagaagttg accaaaacaa
     6601 gaagagaagt cgaaagaaag acgccgcgat aaccaaatgc actggaaaca ctggagccga
     6661 tcataggccc tactacgtta ccagttgctt ggaaagattg gttataactg aagatccgtc
     6721 cagcagcttg atgaggcgaa tattttgtga tcaatgcttg gacagcaggg agcagacaag
     6781 catcagagat accaatcaaa aaacgtaagg cagctaattg ccaaacattc gtgacgaatg
     6841 ccattgggat ataaaccaga atcgcaaaac ccaaaccaat cgcaagtatt ctttcactcc
     6901 caatcttgtc acctaagcga ccaaatcttg gagcggctat caaagtggca atcccaggta
     6961 tcgaagcaat gattccgctg atcaacgtaa cattcccatg tccatgcagc aattggcgaa
     7021 tgtacagact gatgattgga ctaatggaat tgttggatgc ttggatgatc atcgttgtga
     7081 cgaacatacc gatgacgacg tgtggatatt tcaagtcttt gaaaatctgt ttggcagaaa
     7141 ccatgttttc ttttttcact gggacaaatt tttcatggac gaaaaacaga ctcaacaaga
     7201 agacgaaaaa taagatgatc cctgtaatga aaaaggtagg gcgatagcca aacgctgaag
     7261 cagagatccc gccaagaagc ggtccaagta atgttcctgt aacagaacca gttgctaagg
     7321 ttccaagaac ttgaccactt ttttcttttg gcgtcccagt tgcgacaagg gcagtggcat
     7381 tactgatata accagaaaaa atcccttgaa gcagacgcaa tgcgactaat tgatacacac
     7441 tagtcacgca acccatcaaa gaaatgacga ttgccattcc taatgaagca cgcaacagca
     7501 tcaattttct tcctttttgg tcggctaatt ttccccacca aggagagata agcgtagtca
     7561 ccaagaaagt agatgaaaaa gtcaatccgc tccaaaagtt caattgtgac gtactataat
     7621 tacctaacgt atctatgtaa agtgacataa aaggcatgac taaactaaat ccgataccag
     7681 ccataaatgt tccaaaccat aagacaaaca agttttgttc ccaagtttct cttttcctaa
     7741 ataatttttc ctttattctt gcagccaatt agactccttc tttctgaaaa ttttcatcta
     7801 cagctcttta ttataaacta caatatgtag tttatcaatg aaagtctctt ttttctcttt
     7861 ctttttcgtg gtaaactgaa agagtgaagg atttatttta tgagaggaaa catggaaatg
     7921 aaaaaaagaa atctgtctca agagaaaatc atcgactgct ttcgagaact agcagaagag
     7981 atggatgttc agcaaataac ctttcagcat ttagccaaac gcttggatat caaggctccc
     8041 tcgctgtata accatttcaa aaatattcgt gatgtcaaaa cagcgctaac agcgaaactt
     8101 ctgcaggaac ttaatgacca gctgcgacgg gctcttgtgg gaaaaagtgg tcaggaagca
     8161 ctgcagatct atgcagaaac ttatcgatcc tttgcttttt caaatcaagc agtgtatgag
     8221 ttactgatca gtgtaccgca taccaatgaa gctgttttat tagatggaat ccatgaaacg
     8281 aatcagatca ttttgcagat atttgaggct tttcaattaa cgaaagaaga aaaagttcat
     8341 cgaagcaggg aacttcgtag tatgatccac ggttatcttt ccttacgttt tttaggatac
     8401 tttacaaaag aaccaacaat cgatccagaa gacagctatt tttggatgat caataacttt
     8461 atcgctactt tgccttaaac aatcagattc atttgtaaca attttaagat gttcattaaa
     8521 tgaccctttt ataagagaaa attcttgcaa ctttcttgaa aagtgactaa gatgagtcta
     8581 gaaaagctga aagaaggagc acgagtatgt atcaagtaat taccatgttc ggggacaatg
     8641 agccttggtg gttttttgaa gactgggaag aagatattga agaagaagaa acattcgagt
     8701 ctttcgaaga agcacgacaa gcgtacgaaa aacaatggaa cgaaatccat caaaattatg
     8761 aatatatcaa tgcaaaaagt aactttctca gcgccttttg gaatgacgga gatgagcgct
     8821 ggtgtgaaga gtgtgacgat gatttacagc aatacaaggg acttgcgttg ttgaaaaatc
     8881 atcagcccat cacagtagaa agcagaaagg aattttatga aacaactaat tctagcggaa
     8941 aaaccaagcg ttgcgaaaga cctaagcaag gtgcttggtg ctaatcaaaa gcacaaaaat
     9001 tattatgaag gacccaaggt gatcgttact tgggcccttg gtcatttatt aggattgaaa
     9061 atgccggaag atctgaataa ggaatggcag agctggcaaa tggacacgtt gccaatgatc
     9121 cctaaaaaac taggaatcaa accacttcct aaaacaggac atcaattaaa agcaatcaag
     9181 cagctggctc agcgaaaaga tgtctcagaa gcagtcattg cgacagatgc cggaagagaa
     9241 ggggaacttg ttgctcgctg gatcttagag tatgtgcatt tcaataaacc agtgaagcgg
     9301 ttatggatct cttctcaaac agataaagca atcaaaactg gattcaagca actaagacct
     9361 gcgaaagaat atgatgcatt gtatgaatct gctcttgccc gggcaaaagc ggactggctg
     9421 gtaggattga atgtgaccag agccttaact gtgaagtacg cggataattt atcagcaggc
     9481 cgtgtgcaga caccgacact agcattagtg agagaccaag aaaagaaaat cgagcagttc
     9541 cgtccacaaa cgtatttcac gatcacgttg caagctggaa aagaacaagc caaaatgctt
     9601 caaaaaaatc aatttgcttt aaaaacacaa gaagaagcac aaaaacttgt tcaattgatc
     9661 agtacaagca aaggaactgt cacagaagtc caagaaaaat taaaaacgga atctgcgcca
     9721 ttgccttatg atttaacaga gatccaaaga gaagccaatc aacgctatgg ctattctgca
     9781 aagaaaacat taagtcttgt acaaagtttg tatgagaccc ataagatcgt tacttatccg
     9841 cgtacagaca gcaaatatct gacaaatgac atgaaaagta cgatgaaaga acgattgcaa
     9901 gcagtcagcg actttgctcc tgaggtcaag cagtatttga aaaacggagc catcgtccaa
     9961 cagacaaaag tattccaaga cagtaaagtg accgatcacc atgcactttt gccaactgaa
    10021 aacagagcac gttatgaaaa attaagtaat gaagagcgaa aaatctatca aatgattgtc
    10081 tctcgttttt taggactgtt tgctcaaccc cataaagtca gccaaacaaa agttacggtt
    10141 gaatttggta aagaacgatt catttttcgg caaaatcgtg tcgtccaagc aggatggaaa
    10201 ggcgaaactg aaactgagac ggaaacagtc aaatgggaaa aaggaatgag aatcaatccc
    10261 gatttcgcca tcaaaaaaga actatctgca ccgcctaaac cattgtcaga agcaagcttg
    10321 cttggtcaaa tggaaaaata cagtctggga accccagcta cgcgtgcaga gattatcgag
    10381 aaactaatca aatctgaact gatggaacgt acccctcaag gactgcaagt atctcctaaa
    10441 ggtaagcaat tattagtatt ggtaaatccc tctttagtaa caccagattt gacagaaagc
    10501 tgggaaaaag accttgaagc tattgctgca ggaaagaaac aagcaggaac cttcttgaat
    10561 ggaatcgaaa aagaaacaaa acgcttggtg aatgagatca aatcaagcaa acaagaatat
    10621 caagatttct cgattaccca aaagaaatgc ccagaatgtg gggccaatct gcgcgagaaa
    10681 aacacacgtg acggcaaaat ctatgtctgc accaattcag attgcaacta ccgcagaaga
    10741 aaagatccaa aagtctctaa tcatcgatgc ccgcaatgtc accggaaaat ggaaatcatc
    10801 gaaggtaaaa acggtgctta tttccgttgt aaatacgatg gaacgacaga aaaattgctt
    10861 gataaaaaag agcagaagaa gaaaatgacc aaacatgaag aacgacgctt gctgaaaaaa
    10921 tatgcacaag cagaagaacc agaagaaagc ccattagcag cagcactaaa agcagcaatg
    10981 aagaagtaat aagatatgaa agaggttgtg a
//
LOCUS       DAYYHX010000088        10973 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000088 DAYYHX010000000
VERSION     DAYYHX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.089"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1035)
                     /locus_tag="ACHA6Y_001665"
     CDS             complement(<1..1035)
                     /locus_tag="ACHA6Y_001665"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM8987760.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEK"
     gene            complement(1025..1516)
                     /gene="coaD"
                     /locus_tag="ACHA6Y_001666"
     CDS             complement(1025..1516)
                     /gene="coaD"
                     /locus_tag="ACHA6Y_001666"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM8987761.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(1513..2073)
                     /gene="rsmD"
                     /locus_tag="ACHA6Y_001667"
     CDS             complement(1513..2073)
                     /gene="rsmD"
                     /locus_tag="ACHA6Y_001667"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM8987762.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(2192..2530)
                     /locus_tag="ACHA6Y_001668"
     CDS             complement(2192..2530)
                     /locus_tag="ACHA6Y_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM8987763.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(2514..2960)
                     /locus_tag="ACHA6Y_001669"
     CDS             complement(2514..2960)
                     /locus_tag="ACHA6Y_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8987764.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(2941..4065)
                     /locus_tag="ACHA6Y_001670"
     CDS             complement(2941..4065)
                     /locus_tag="ACHA6Y_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM8987765.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(4086..7514)
                     /locus_tag="ACHA6Y_001671"
     CDS             complement(4086..7514)
                     /locus_tag="ACHA6Y_001671"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM8987766.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(7540..8703)
                     /locus_tag="ACHA6Y_001672"
     CDS             complement(7540..8703)
                     /locus_tag="ACHA6Y_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8987767.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(8706..9008)
                     /locus_tag="ACHA6Y_001673"
     CDS             complement(8706..9008)
                     /locus_tag="ACHA6Y_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM8987768.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(9122..10894)
                     /gene="recQ"
                     /locus_tag="ACHA6Y_001674"
     CDS             complement(9122..10894)
                     /gene="recQ"
                     /locus_tag="ACHA6Y_001674"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM8987769.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
BASE COUNT         2983 a         2411 c         1879 g         3700 t
ORIGIN      
        1 tttctctaga tagtcaagcg cttcttgtac tgtttttact ggtacgattt tcattgagga
       61 attcaatttt ttagcggcag cttttgcttc ttcgtagttg gtttttattt caggatattc
      121 tttcttaaca tcgtctgaaa tcgtatcatc tggtgcaaag aaaatctcta cgccagcttt
      181 atccgcactt gccacttttt tatcgattcc tccgattcgg ccaacttctc cttcactgtt
      241 gatcgttcca gttcctgcaa tttgcttacc atgtcgcaag tttttaccag tgacttgttc
      301 atagatttct aaagtgaaca tcaatcccgc tgatggccca ccgatagagc cagaattaat
      361 acgaacatct gtatctgatt cgatctcggt atggtcagta agcgtgatac ctattcctgc
      421 ctttttatct gttggtaact cgatcagatc accagaagct gttttttctt caccattatg
      481 caaataactg atttcgattg tttgtccaac tttttggctt tttacataat ccataaatgc
      541 ttctgtactt tgaaagcttt tcccgtctac ttttgttact gtatctccaa ctgaaagctt
      601 gccaaagaaa ttcgatgttt tctctacact catgacatat acgcctttga attccatttt
      661 ataaggcttg tctgcaagtt ttaacgcttg ttcgattgct gcattttggg aagaatccat
      721 ataatactgt tgcatttgat tatattcttc atttgtcgcc ccaccagtca attcttcttc
      781 actgatcaat tcttcaaaag gggtcatttt agcttttaaa gctaagaaac ctgtagcttg
      841 cctgatacca acagaggtaa gagagaaaga cccagaacct ttatcttcct tatcattgac
      901 cgtaattagt tctttcagat cgatcgtggc tcccggtgat tctacataat acggcaaggg
      961 aactactaca aatccaatga tcaacattcc aaatatgata atcagtactg ttttcaacca
     1021 ttgtttagct ttcatttgat tcacgctttc cagctaaagc ttcgttgatc actggcggta
     1081 agtagctgct cacatctccg ccaaagtgca gtacttcttt taaaatactg gaactcacgt
     1141 gactatattc tggttttgct aataagaaga cagtttccaa ttcatcatat agatgatgat
     1201 tcatctgagc aatctctcgt tcatattcaa agtctttgat acttcgtatg cctctgatca
     1261 acgcatctgc accaatctct tttgctgttt ctaccgtcaa ttgattttct tgatgcatga
     1321 cttttacatt aggaagatga gaaacagctt ttgtgatcaa tgtcattctt tcttctgggg
     1381 gaaacaaact tttctttgaa gtattgataa aaacaccaat tactacctca tcgaataatt
     1441 tagccgcacg ttccaccgta tctaagtgac cttttgtaaa aggatcaaaa cttcctggga
     1501 ataatgcacg tttcattgat ttccctcttt tctatagata gttacggccg ttatgccata
     1561 aatctgtttt cttatttgat ggaatccagc cacagtttct ggcaattcga ctgttttatc
     1621 cgtttcgcat acgataactg cggttttaga aaaaagtccc agttcttcca ttttatggat
     1681 ttgatcgatt attttttgtt ttgcatatgg cggatcaaga aacaagtagt cgaattgttg
     1741 tttctccgct gctagccgtt cgattgcttt gtccgcatca agttttattg tggtaaattt
     1801 ctctggttct ttcgtaattt cgatgttttc tttgatgatt tttagcgctt ggtaattttt
     1861 gtctacgcat accgcatgac tgcagcctct agatactgct tcgatagcta aaccaccgct
     1921 tcctgcaaac agatccaata ctgtctctcc atcaaaatat ggtccaatca tattgaatat
     1981 ggattctttt actttatctg tagttggtct ggtgttcgcc cctgctaaac tttttaaacg
     2041 gcgcccgcca taatttcctg atattatccg catctactca ctccttagaa atggtactgg
     2101 tactagtata ccaaaaaaat acttggacaa tgatcaaaaa tcgatcgtta tccaagttat
     2161 tttttattta aaaactgctc ataagagatt tttaaagatg ttcagctaat cggatttctg
     2221 tatttagctc ttcaatttcg aaaccttcgt cttttaatac tgttttgttt cctatttttt
     2281 cggcaaaatt catttcgata tccggtcggt aagaacgttc tacgtttcga acaaaatgta
     2341 atttacggat tcgttcttct gtttcttcca atgtctcttc atttaagtaa agcacgacat
     2401 atttcattct tcttgagaca taatgcaaca agccgaactt tttcagattc tttaattgtt
     2461 ttaagctgta gacccatacg atcagcccgc gtcgttttgt tatttcaaat tcactattgt
     2521 ctaattgcat gacaattacc tccgcatcca aagtgttttt tcgtttcaaa aaacgggcta
     2581 cctgcatcta ctttgatttc attagaaact tcttcggcaa gtacttggac aatttgatcc
     2641 aacagacttt gcagctgtgt ttcactatat cggaaagctg caacatgctc atccatatca
     2701 agctgtcttt ttgcttttcg aacagctcgt tttttttcac gataatcagg tgcatactca
     2761 ccatatgccg ctacattttc aaatgcttct ttcttagaca agaacgcttc tttcaactgg
     2821 caaacttttg cagattcttc cattgactgt ttatgttcaa gataagaagc catcgtttta
     2881 ctctttttta tcatttcagc gacttggagg cattgatcct ccagttgaaa cagctcttcg
     2941 ttaacgatca ctgctatcac tactttctcc gttttcttct tgcaataaga ctaacatatt
     3001 ttctttagaa aaagcaatgc ctacagaatc cgttcccggg tccatgaaac ggtcatggtt
     3061 gtaaggatcg ctgtaccaat ataaaaacga aaaagtagcg tcaatcgccg gatgaataaa
     3121 tagcccacta gtattttcta atttttgttt actgattaac ttctccaatt catcattcgt
     3181 cagtgagaat ttcactgtcg tttgagcact gcctagcgat ttttgccaat cagccatacg
     3241 ttcctctgac aatatttcct caggccttgt caaaaaatca tgtagtagca gctgactgtg
     3301 gttttgaaag gcaagtgttt gattgtatat gggaagttcg tcttctcttc taagcatatt
     3361 taataaagtc gtactttttc gttctttaac gcgattgatt gattcccaat cagccgattt
     3421 atctgcttga taatgtggaa ggccttcacc aaacacttga tatggcaata gtttcaaaag
     3481 actatcttta tctaaataca caactgaaaa taggccgttt gttgtttgat caaaaaataa
     3541 aatagcaaaa gtgtcgttat caaaagcaat tagcggacga taattcatat cctcttccat
     3601 tagttcgatt ccaacttctg tatcatggta ctcaaaagtg aaattagtat agatagtagt
     3661 gattttcgta agatcttgca tcgtcatccc taaataaaac ggcgcgatat cagcaggccc
     3721 tgtttgcagt actttaacag ttgtgactct gctgtcttgg atgttcgctt caaatgtgag
     3781 atgactagct ttattttcat aatatcttgt ttcaaaacca aatccgctag ttaatcgatc
     3841 ataggcttgg ccaaaatcct tttccagctg atcgatcggt tttccgatat aagtagaaaa
     3901 accagttgct ttaagctcct ggtatttcca cgttgtcatt tggtttgata tttgttgatc
     3961 agagttttcc ttcacttcaa gttttggcgg aaacaagact ggttccatgt agaatgccac
     4021 aataactaat agcagaatca gccaaaacaa acccagtctt ttcatgtttc cccacctttc
     4081 gcttattatt tctcagtcac ttcaatcaaa aggtcaccag acgaaatagt gtctcctgaa
     4141 gacacatata catgtgcgac ttctccgtca aaacgtgctt cgatggccgt ttccattttc
     4201 atcgcttcag taatcatcaa cgtatcacct tttttaacgt tatcgccttt tttcactaat
     4261 acatccaaaa cagaaccaga catcgttgct ccaatttgtt ctcggtttgt cggttcagct
     4321 ttcaatcggg tttggaccgt actgatgatc gaattatctt tgatagtgat ttcacgtctt
     4381 tgtccattca aattgaagaa cagtacgcga ttcccttcga tatcgggttc tccgatttca
     4441 tctaaacgaa tgataagcgt ttttccgcgt tcgatttgta cattgattgt ttcacccaaa
     4501 cgtatacctt ggaaaaatgt tggtgtgtcc agtaatgtaa catcgccaaa ttgattatat
     4561 gctttttgat agtccaaaaa gacttgcgga tacatcaaat agcttaatac ttcgtcttgt
     4621 tttggttcat agccaatttt ttctgccaat tcttttttta cttcgttgaa gtcgactgat
     4681 tcagccaaaa gacctggacg ttcattcaat gcaggtctgc ctttaagaat aatcttttgc
     4741 aattttttag gaaatccgcc aacgggttgc cctagttctc cttggaagaa agtcacaaca
     4801 gattcaggga aacttagtgt ttcgcctttt tcataaatat cttcttctgt aagatcattt
     4861 tggaccataa atagtgccat atctccaact actttagatg atggagttac tttaacgata
     4921 tcgccaaaca tcaagttgac agtatgatac atttgtttga tttcatccca gcgatgacct
     4981 aatccgacag cttttgcctg ttgctgtaga ttcgaatact gtcctcctgg catttcatgc
     5041 atatatactt ctgtttcagg agcattcaag ccgttttcaa acggtttgta atacatgcgc
     5101 acatcttccc aataatggtt taatttctgt gcattttcaa tcgtgatttc tggcagccgt
     5161 ggaccattga ccagtgcata atacaagctg ctcatgctag gctgactagt attcccactc
     5221 attgcactca ttgccacgtc aacgatgtct actcctgctt ttgaagcagc agaataagtg
     5281 atgattccat tcccgctggt atcatgagta tgcagatgga ttggcagatc tgtcgtttct
     5341 ttcaattcac taatcaaacg ataagctgcc tgcggtttaa gcagcccagc catatctttg
     5401 attgcaatga tatgcgcacc cagttgttct agttcctttg ccatgtcttt ataatactga
     5461 acattgtatt ttgcccgact tggatcgttg atatcgccag tatagcaaat agctgcctct
     5521 gcgattttac cagtatcccg tacagcttgg atactttttt ccatttgtgg agtccagttc
     5581 aaactatcaa agattcgaaa tacatcgatt ccttgcgctg ctgcttcttt gacaaattcc
     5641 accaatacat tgtcaggata attagaatac ccaacggcat ttgaacctct gaaaagcatt
     5701 tgcagcaacg tattaggcat taaagaacga atttttctca accgttgcca aggatcttca
     5761 ttcaaaaagc ggtaagccac atcaaatgtt gctccccccc acatctcact tgaaaagagt
     5821 tcaggaagac cttctccagt caaacgtgca atctgcttga aatcctttgt ccgaacacga
     5881 gttgctagca gactttgatg ggcgtctcta aatgtcgtat ccgtcaacaa caaattttcc
     5941 tgtcctttga tccattcgac caaagcatcg gcacccttcg catctaatac attcttagcc
     6001 gttacgtaat cagttcttgt gattagatct ttaggcatac gtggttcttc ataaaatggt
     6061 ttttcgccac tttcgatacc tggaaaacca ttcacagtaa tttcaccgat atatttcatc
     6121 gttttgtttc cgcggtctcg tagtcgagga aattcaaata atgttgctgt actatcaatg
     6181 aaggttgttt ttgcatttcc tgattggaat tcaggatgtg tgatgacatt gagcataaat
     6241 ggaatgtttg ttttcactcc acggattcta aattctttca agcaacgttc cattttctga
     6301 atggctgttt caaaggtagc accatgcgta catactttta ctagcaatga atcaaaatac
     6361 ggtgtcacta catagcccgc gtaagcattt ccgacatcca gacgtacgcc aaaaccacca
     6421 ggtgaacgat acgtatcgat tttccctgtg tctggtaaaa agttattcaa cgggtcttct
     6481 gtagtgattc ggcattgaat ggctgaacca tttaatttaa ttccttcttg ctgaggcaat
     6541 ccgatttctt tatgcagatc caaaccttga gcaatcaaca gttgcgttgt tacgatatca
     6601 atatctgtaa tcatttctgt gatcgtatgt tccacttgga cgcgagggtt gacttcgata
     6661 aagtaaaaat catctccttc aacaagaaat tctactgtac ctgcgttgac atacccgaca
     6721 tatttcatca attggacagc tgcctggcag attttttgac gttgctgttc attcatagag
     6781 acacaaggcg ctacttctac tactttttgg tgacggcgct gaacagaaca atctctttca
     6841 aaaagatgga tgacgtttcc atgcgtatcc ccgagaatct gtacttcgat atgcttagga
     6901 ttagcgatgt atttttcaac atacacttca tcactaccaa aggctgcttt tgcttcgctt
     6961 ttcgcacgtt cgtagccctc tcttgcactc ttttcatcgt gagctacacg catgccgcgt
     7021 ccgccgccgc ctagagctgc cttgatcata atcggataac cgtattgttt cgcaaactcc
     7081 aaggcatcgt ctatagaagc gattggtcca tctgtgcctg gaatcgaagc aatccccgct
     7141 tcgatagctg cagcttttgc tttgatttta tctccaaaaa tatctaaatg atgcagtttg
     7201 ggtccaacaa aaatgatgcc ttcttcttcg caccgttgcg caaaattcaa gttttccgac
     7261 aaaagaccgt aacccggatg aatagcgtca gctccgcatt ctttcgcgat agaaatgatc
     7321 ccttcaatat ctagatacgc atcaatcggt ttttttcctt gtcctactaa atatgcttca
     7381 tccgctttga aacggtgtac ggaatattca tcttcttctg catagattcc aacagtttta
     7441 attcctaatt ctgtacaagc acgaaataca cgtacagcga tttcacccct gttagcaact
     7501 aaaacttttt tcatcgaacc actcctacat ctttttttat tataaaccta agctcttgcg
     7561 tttttcatcc gcactgatgt tgaggacaaa gccgacacaa atcgaaagca tcagcaaact
     7621 ggagcctcct tgacttaaaa atgggaacgt gatccctgtc aaaggaataa cgccagtaat
     7681 tcctccaaga ttgacaaata cttggatcaa aaacattgcg ccgatcccaa tgcacaaaag
     7741 cgaattgaac ggatcttttg aacggatacc tacaaggaca atgcgcacaa tcataaagaa
     7801 tagcaacgct aatatcaaaa tccccataat cactcccagt tcttccacta cgattgcata
     7861 aataaaatcg gtttgtgctt cttgtaaaaa tccttttttc tggatactgt tacctaatcc
     7921 cctgccaaat agtcctccgt tgaacatggc atagtagccg ttgactagtt gatgcccttt
     7981 atttaattcg tctgagaaag gattttggta aatagcaaat cgattataga tatactgtaa
     8041 acgtccgggt aagatctttc cgtttgtgat attgatcagc cagatcgtaa aagtacttag
     8101 acaaaagcct cctactccta caatcaaagt atacacataa ttgactccgc tggctaaaag
     8161 cagaaccaaa ataatcaaaa agataactgc cgcattacca aaatcgggaa gaatagcgac
     8221 taatgcagta agtccgataa ccattgcaag tggacgtttc acagtcccaa gaaaatcttt
     8281 ttgtacactg ttttgtcttt tagataaagt cagagacagg taccagatag aaatgatttt
     8341 taggtattcg gcaggttgga tcgagaatcc tgcaatttgc agccaacctt tcgccccatt
     8401 gatctcctta ccaaaaaaca caacaatcag cagcaaaatc gtcaaaactg cgatggctgc
     8461 catgatcaaa cgctggtttt tcaaaacatc tgttttcatt ttataaagta gtgcgatagc
     8521 aatcaagcta agcacccaaa aaatactctg gttaatgact gatgcggacg gattttgtcc
     8581 gttttctaat aatagatacg acgtagaact atataccatg atcaatccca atacacataa
     8641 aattaaatag ggaatcaaga tgctatagtc taatagatgc ctttttttca ctttcttagg
     8701 caagtctatt cctccttgcc aacttctttt ttatcataaa tttcttggta aacctcacta
     8761 tataactgat tcaattctcg ttctagctca cttaacatca gctggccctc gttctttccg
     8821 attactccta tacggacagc aaaaccgact tgccgggaaa agccgtacat ctgtgtatct
     8881 accacttctt cgaaggcttt gcattgtgag atacagagac tgttgttttg atttcggatt
     8941 aatcgtttga tccgttctgc ttcatcattt aatacttgga tcgcaagttt tttcgacaaa
     9001 gaatccatct gtgcttacct cccattctaa ttccttaatt ataacatatt tgattttcat
     9061 aaggagcaaa aaaaacagat ttaaccagtt gtttttattc atttttacgt attcgttctt
     9121 tctacttatc cagaacgtta gagtccgcat tttttatccg agataaaaag gcttcgccat
     9181 atttatcaag cttgctttgg ccaactcctt ttatctgaag catctccaat ctgcttgatg
     9241 gacgtattcg gctcatttct ttcaatgtgg aatctgaaaa tacgacatac gggggaacgc
     9301 ctgcttctga tgctagatcc gttcgcagtt cccgcagcac ttcaaatagt gtatcggttt
     9361 cttcatccga tagctgctgg actttcttgg gttcctttcg ataaacagat tcttgtcctt
     9421 tcaatacttg tatccctctt tctgttactt tcaaaacagg atactcacca ttgatggtca
     9481 gaagatagcc attcgatatc aaatattcaa ttaactgcat cgtttctttt tgcgattggt
     9541 ttttcatcag tccataggta gataaatgtc caaaacctaa agcttgcagc ttttgttctt
     9601 tcgatcctgc taagactttc atcagcattt gtttaccata gttctcaccc atacgtttta
     9661 agcaagaaag aaccatctgt gcttctgtcg ttacttctac tatttctctg tcatctaaac
     9721 agttaccaca acggttgcac ggactgcttg tctcgccaaa atagtttagg atgtattgct
     9781 gaagacagga ctcaatatac acgtattctg tcatcgcttt taatttttca tattcttttt
     9841 gcttttgccc ttcttctctt tgggactgct ggataaaaaa cttttggact tggatatctt
     9901 gaggagagaa aagcaggatg gcttcactgg aaagaccatc tcttcccgcc cgaccagctt
     9961 cctgatagta tgcttctaga gagccaggaa tttgataatg gataacaaaa cggacattgc
    10021 ttttatttat acccatacca aatgcatttg tcgctacaat cagctgcaag cgatcgtata
    10081 aaaaggcttc ttgcatttct gttcgctcat tctcatttag accaccatga tatctaccca
    10141 cggaaaaatc gaatttttta agcaaatgat agagacggtc tacttctttt ctcgttgctg
    10201 cataaatgat accagattga tttttgttga tctttaaata ttcgattaga tattgttctt
    10261 ttttttgatc tttaattact tgaaatcgta gattttctcg ttcaaaacca gtctgaatat
    10321 ggttgttttc tggaattttt aaaagtcttt ttatgtcagc agctacttgc acagtcgctg
    10381 tagctgtcaa agcaacaatt acaggacgat ttggcagttg atctagctgg ttcgccatct
    10441 gtaaataact tgggcggaaa tcatgccccc actgagagat acaatgtgcc tcgtcaatag
    10501 cgatcatacg gacgttccat cgcttcattg cttggataaa atccggcatg ataaaacgtt
    10561 caggagcgat ataaagtaaa cggtactgct gtctatcgat ttcttggaag cgtctagctg
    10621 tttcgtaacc atctaacgag ctattgataa aagtggcggg tatacctaat tgattcgccg
    10681 cgtccacctg atccttcatc aaagagatta aaggggacac aaccacagta agcccatcta
    10741 atagtaaagc tggaagctgg taacagattg atttcccact tccggtaggc atgattccta
    10801 atacattttc ctgatcaatg actttctgga taatttcttt ttgaccaggt ctaaactcat
    10861 catagccaaa gtattgtttt aataagtcgt tcatcttccc gctcctttct cttgccattt
    10921 tacttcgatt ttcttcgaag gacaagttga tctcctttcg tgaaacatta tct
//
LOCUS       DAYYHX010000089        10959 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000089 DAYYHX010000000
VERSION     DAYYHX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.090"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(166..773)
                     /locus_tag="ACHA6Y_001675"
                     /pseudo
     CDS             complement(166..773)
                     /locus_tag="ACHA6Y_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1257..1484
                     /locus_tag="ACHA6Y_001676"
     CDS             1257..1484
                     /locus_tag="ACHA6Y_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM8987770.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1500..2666
                     /gene="hemW"
                     /locus_tag="ACHA6Y_001677"
     CDS             1500..2666
                     /gene="hemW"
                     /locus_tag="ACHA6Y_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM8987771.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            2900..3943
                     /gene="hrcA"
                     /locus_tag="ACHA6Y_001678"
     CDS             2900..3943
                     /gene="hrcA"
                     /locus_tag="ACHA6Y_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM8987772.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4037..4600
                     /gene="grpE"
                     /locus_tag="ACHA6Y_001679"
     CDS             4037..4600
                     /gene="grpE"
                     /locus_tag="ACHA6Y_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM8987773.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            4651..6480
                     /gene="dnaK"
                     /locus_tag="ACHA6Y_001680"
     CDS             4651..6480
                     /gene="dnaK"
                     /locus_tag="ACHA6Y_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM8987774.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            6631..7797
                     /gene="dnaJ"
                     /locus_tag="ACHA6Y_001681"
     CDS             6631..7797
                     /gene="dnaJ"
                     /locus_tag="ACHA6Y_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM8987775.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7879..8508)
                     /locus_tag="ACHA6Y_001682"
     CDS             complement(7879..8508)
                     /locus_tag="ACHA6Y_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8987776.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            8520..8693
                     /locus_tag="ACHA6Y_001683"
     CDS             8520..8693
                     /locus_tag="ACHA6Y_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987777.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(8738..9475)
                     /locus_tag="ACHA6Y_001684"
     CDS             complement(8738..9475)
                     /locus_tag="ACHA6Y_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8987778.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(9477..>10959)
                     /locus_tag="ACHA6Y_001685"
     CDS             complement(9477..>10959)
                     /locus_tag="ACHA6Y_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8987779.1"
                     /translation="HSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQKIEPKKDV
                     YVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAALQALESGQ
                     ADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTVGAKVGTE
                     SAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQGQELETPI
                     KKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSETSSESSTA
                     DESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRALRTIASI
                     YVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIVRGGINAV
                     PVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISAIGVVELL
                     QTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
BASE COUNT         3584 a         1972 c         2277 g         3126 t
ORIGIN      
        1 acctcattat ttttttaata ccagtatagc ataatcctga atttttgaat ccgtaaaacc
       61 ttgtctatga atataaatag agtgtatctt ctttgataaa tgtcagaaaa agtgtgacaa
      121 ataatcatca gtaaaattac ttgtcacact attgataagg agtatttaat tgaagagaat
      181 gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag tttgtataga
      241 cgaatcgttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag aatcaagtct
      301 gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt cacagcttgt
      361 tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg aaattgcgca
      421 tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc aactgcccca
      481 caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg atataaatga
      541 tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac tttttcaggt
      601 atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt gacctctgat
      661 tggatagaag cagttttgtt ttgccatttc tcattttggt ttagtgtgct agtaatataa
      721 gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt cattttcaag
      781 ctctccttta ctatctataa caaggacaac agttttaacc tacagacatt cattaaaatt
      841 gggtagcgac agcgaaaaca acaattctta agttttagac agagtataca gatagtcaac
      901 ttaattaaat acgaactaaa taaaaatgac aggtaatcat ttttgtttac cagcattata
      961 acattttgca tacatggatg acaaaagaac caattcccac ttgcttgagt acaatattaa
     1021 aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat cgggcactca
     1081 gacatgaaaa caaccctaga gatatataac taggtaacca gtttaatgaa agaaaaaatc
     1141 atccaagaag tcgattcttg gattttttag tggatttgtt cgccaaatac gtttagtatg
     1201 cgtatatgag aggttgtaag caaagcgttg tggtctgagc acttaaggag gaatctgtga
     1261 aaatagcttt tcatattttt gcagattttg acttaatgcc gaaggtcagc tttgcgatta
     1321 ccgtttatcg agaggctgta aaagcaccgt gtagctccga tgacaataag caagcatttt
     1381 tagagctagg tttccttgat tctaaatatg cggcttattt caatgagttg gtgcttgaac
     1441 gcgattttaa gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa agaatttcaa
     1501 tgatagaaac aataaatccc gcagaaaaca aggtttctgc gtacattcat atcccatttt
     1561 gcgagcatat ctgctattac tgtgatttta ataaagtttt tttagaaggg cagccagtag
     1621 atgagtatgt ggaaatgctg ctgaatgaaa tgaaaataat gctagaaagt catccgacag
     1681 cgaatttaga aacactttac gtagggggcg ggacaccaac ttctttatct gctaaacaac
     1741 ttgatcgact gttatcaggt gctagagaga tcctgccttt tcaaaatggt aatgagttta
     1801 ctgtagaagc gaatccaggt gatttgaccc gtgaaaaact tcaagtaatg aaaaactatg
     1861 gcgtcaatcg tttatccatg ggagtgcaga catttgataa tcgcttattg aaaaaaattg
     1921 gacgtaaaca caccgcagaa gatgtgtatc aaactatgca atttttagaa gcagaaaatt
     1981 ttaccaatgt aagcatcgat ttgatttatg cattaccggg acaaacacta gaaggatatc
     2041 tggatacttt ggaaaaagct ttggctttgg atctgcctca ctattctttg tactcattga
     2101 tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca aggaagactc gaactgcctg
     2161 atcaggaaac ggaaaccaga atgtttgaag agacgattga agcaatggaa aagcatggac
     2221 gtcaccaata tgaaataagt aactttgggc tctccggaca tgaatctcaa cataacctca
     2281 tgtactggaa caatgcccac tattttgggt ttggagctgg tgctagcggt tatcttggag
     2341 atattcgtta ccgcaacaaa ggaccaatcc aacactattt acgcccatta cgagcaaatg
     2401 aacgcccggt attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa gaagaaatgt
     2461 tcttaggact aagaaaaaaa acagggatta ctaaaaaaca tttctatgat cgctatcaaa
     2521 aaacaattga atcggtgtac ggtgatgtgc taagggaatt agagcaagaa gggttacttg
     2581 ttaatgaaga agaccgtgtt tatttgacac caagagggac ctttttgggg aacaatgtgt
     2641 ttgagcgctt tttgtttgaa aaatagttta aagaatattt gttttttatc tgaatttaaa
     2701 taaaaaaagt tcaaaaatac aataaaggac tattgaaacg ttgatataac tggatttaca
     2761 tcaattattt agaattagca ctcttgtgtt tagagtgcta aaaaatggag tctttctctt
     2821 gacaggagaa gggcttcttg ctatatttat aagtggatta gcacttagaa tatatgagtg
     2881 ctaatgagag gtgaggaaga tgttaacaaa gcgacaaaac gacattttac gtctgatcat
     2941 ccaacactat acgagtagtg gcgtgcccgt tggttccaaa acattgatgt ctgagggagt
     3001 caaggccagc tcagcaacga ttcgcaatga tatgaaagca ttagaagatg aaggattgct
     3061 gttgaagact cactcttctt ctgggcggat tccatctgct ttaggctatc gttattacgt
     3121 ggatcatttg ttaaaacctg ctcgcgtagc agaagatgat ttgctgctga ttcgtcagtc
     3181 tctaggcaga gaatttcatg aaatcaatga gattattaag caatctgctg agatattatc
     3241 tgagctgaca agctatacga ctttttcatt gggacctgaa ataaaagacc gccgactgac
     3301 tggctttcgt atcgtaccct tgaataatcg acagattatt gctatcgtcg taacagatat
     3361 ggggaatgta gaaagccaag tgttcactct tcccgaatat atgggaagtc aggaactaga
     3421 aaaaatggta cagattgtaa acgaccgtct agtcggagat cctctgatga cggtgtacca
     3481 taaacttcgt acggagattc cgatgattct tcataaatat ttccaaacga cagaagggat
     3541 gtctcagtta tttgacaccg ttcttggtca tgtttttgaa gataaggttt atgtcagtgg
     3601 acaaatgaat ctcttggatt atgagccccg tcaagatctt gatcagttca aatcgatgtt
     3661 ttcttttatg aaagactcag acggacttac acaaatgatc actccgatgg atagcaatat
     3721 tcatatcaag attggtgctg aattaggcaa tgatttattg cagaatatga gtatgattca
     3781 agcaagttac gagatagatg gtcatggaag aggtacaata gccttgttag ggcctactag
     3841 tatgccttat tctcggatgt tcggtctagt cgatgtttat cgaagagaac tagcagctaa
     3901 acttgctgat tactaccgtt cgcttgactt gtcaaactcg taatgtgaat aacgaagcta
     3961 tttgcagctg atcgttctct ctttcttagt aagagcaatc aaacagctga ctgaataatg
     4021 aaaggaagac tgtatcgtga aagaaaatca agaagaacta gataaagaaa tgacagatgc
     4081 tcagccagaa ccggaaatcg atgtcgaagc tgcagaagat tcgggtattt cagaagctga
     4141 agcggaagaa ttcgaaacag cgaaattaaa agctgagtta gaagaaatgg aagataaata
     4201 cttgcgggct cgtgctgaaa tcgcaaatat ggcaaatcga ggaaaaaatg agcgtgagca
     4261 attacaaaaa taccgttcac aagatttagc caaaaaatta cttccatcaa ttgacaacct
     4321 ggaacgtgca ttagccacag aagtgtcaga tgatcaaggg gcaggtttga aaaaaggcgt
     4381 ggaaatggtc ttggaaagtt tgcgtaacgc actggaagaa gaaggaattg aaaaaatccc
     4441 tgctaaagga gaagcctttg atcctaatct tcatcaagca gtacaaactg ttccagcgac
     4501 tgaagataca ccagccgata cgattgtaga agtactacaa gaaggctaca aattacatga
     4561 tcgcgtgtta agaccaacca tggtcattgt tgcacaataa ttgtgcggat ataaaactta
     4621 cactttcaat taaagaagga gacgttaatt atgagtaaaa ttatcggtat tgacttaggt
     4681 acgactaact ctgcagtagc agtattggaa ggaaacgaag caaaaatcat cgctaaccca
     4741 gaaggaaacc gtacaacacc ttctgttgta tcattcaaaa atggagaaat ccaagtaggg
     4801 gaagttgcaa aacgtcaagc tgtgacaaac ccaaatacga tttcatcaat caaacgccat
     4861 atcggtgaag caggttacaa agtagatgta gaaggtaaat catatacacc acaagaaatc
     4921 tcagcaatga tccttcaata tatcaaagga ttcgcagaag actatttagg tgaaaaagtc
     4981 gaaaaagcag ttatcactgt gccagcttac tttaatgatg cacaacgtca agcaacaaaa
     5041 gatgctggta agatcgctgg tctagaagta gaacgtatcg tcaatgaacc aactgcagct
     5101 gctcttgcct atggtttaga taaaacagat cgcgacgaaa aaatcttagt attcgacctt
     5161 ggtggcggta cttttgacgt atctatcctt gaactaggag acggtgtctt tgatgtattg
     5221 tcaacagctg gcgacaacca tctaggtggg gacgactttg ataacaaaat catcgactac
     5281 atggtagcag aattcaaaaa agaaaacagc attgacttgt ctcaagacaa aatggcttta
     5341 caacgcttga aagacgctgc tgaaaaagcg aaaaaagatt tatctggcgt atcaagcaca
     5401 caaatcagct tgccatttat cacagcagga gaatctggac cattgcattt ggaaatgaca
     5461 ttgacacgtg caaaatttga tgaattaaca gctgacttag tagaacgtac aaaagtacca
     5521 gtacgtcaag cgctaaaaga tgcaggttta tcacaatcag aaatcgacga agttatttta
     5581 gtcggtggat ctactcgtat ccctgcagtt gtagaagcag tacgtaaaga aacaggaaaa
     5641 gaaccaaaca aatcagttaa ccctgatgaa gtagttgcta tgggtgctgc tatccaaggt
     5701 ggcgtgatca ctggtgatgt gaaagatgtt gttttacttg acgttacacc attgtcatta
     5761 ggtatcgaaa caatgggtgg agtgttcact aaattgattg accgcaatac aacgattcca
     5821 acaagtaaat cacaagtatt ctcaactgcg gcagataatc aaccagcagt agacatccat
     5881 gtattacaag gtgaacgccc aatggctgct gacaacaaaa ctctaggaag attccaattg
     5941 acagatattc cagcagctcc tcgtggtatt ccacaaatcg aagtaacatt cgatattgat
     6001 aaaaacggga ttgtaaatgt atctgcgaaa gatctaggta cacaaaaaga acaaaaaatc
     6061 acgatcaaat cttcttcagg tctaacagac gaagaaatcg aacgtatggt aaaagatgca
     6121 gaagcaaatg cagaagcaga taaagctcgt aaagaggaag ttgacctacg taatgatgta
     6181 gatgcattgc tattctctgt ggacaaaaca ttgaaagaat tagaaggcaa agtggacgaa
     6241 gaagaagtga aaaaagcaga aacagctcgc gatgaattga aagccgctgt tgaagcaaac
     6301 aacatcgaag aaatgaaaac taaacgcgat gcattgaacg aaatcgttca aaacttgact
     6361 gttaaactat acgaacaagc ggcacaacaa caagctcaag aaaacccaga agcagctcaa
     6421 ggcggagcag atgatgtcgt tgatgctgat tttgaagaag tcgatggcga cgataaataa
     6481 tcagttcact gaatttcgag agaaggccaa agttgctact ttggcttttt cttttgaaaa
     6541 atagtttgaa gtatcggttt ttgtttcaaa caaaatatga tatgataaca agcgacgtga
     6601 aaatgatgag aaccaatagg aggaaagcct atggcaacaa aacgtgatta ttatgaagtc
     6661 ttagggctct caaaaggagc atcagaagat gaaataaaga aagcttatcg taagctttcg
     6721 aaaaaatacc atcctgacat caataaagaa cctgatgcgg aagaaaaatt caaagaagta
     6781 tcagaagcat atgagatttt aagtgacccg caaaaacgcg ctgcatatga ccaatacggg
     6841 catgcaggca cagatccaaa ttacggtgct ggtggagctg gtggcggatt tggtggattc
     6901 ggcggcttct ctggtggcgg cttcggtggt tttgaagata tcttcgactc tttctttgga
     6961 ggaggcggtc ggtcagtcga tccaaatgcg cctcgtcaag gtgctgattt gcaatatacg
     7021 gtcgacttgt cttttgaaga agcgattttt ggtgtagaaa aagaaatcaa gtataatcgg
     7081 gaagaaatct gtcatacttg tggtgggaac ggtgcaaaac caggcacaca accaactact
     7141 tgtcataaat gtcatggtgc aggtacgatt aatgtagaac gccaaacacc acttggacga
     7201 gtgatgagcc gtcaaacttg tgatgtttgt catgggacag ggaaagaaat caaagaacct
     7261 tgtccaacct gtcatggctc aggacatgag aagaaagcac attcagttaa agtgaatgta
     7321 ccagctggtg tagaagaagg acaacaaatg cgtttagctg gtcaaggaga agctggagaa
     7381 aacggcggac catttggtga tttgtatgtg gtcttccgtg tagaagaaag tgatatcttt
     7441 gatcgtgaag gttctgaaat ttactatgaa ttgcctttaa gttttgtaca agcagctttg
     7501 ggggatgaag tccaagtacc aactgtccat ggagatgtga aattgaagat ccctgctgga
     7561 acacagacag gtacgaactt ccgtttacgt ggaaaaggcg ctccgaagtt acgtggtggt
     7621 gcaacaggcg accaacatgt caaagtaaaa ctgatcacac cgaaaaatct aaatgaacaa
     7681 caaagagaag cattacgtgc attcgctgaa gctggcggca tcaaagttga agaacagcaa
     7741 gaagacggtt tcttcgataa gtttaaagat gcttttggcg ggaaaaagcg gaagtagttt
     7801 ttctagtaaa aacgctaagt cattaataat taaaaaccag acgaacaatc atatgttcgc
     7861 ctggtttttt tgtagttgtc atgtttgtac caacaaaaaa gccagcttta gaatatgata
     7921 atcgtcatag tttctagggg tataaccagt gagagaatgg aacttgttca atcgatattg
     7981 caaggtgttt ttatgaataa ataattcttc tgagcaattt ttgatgcttc cgttattttt
     8041 actgaagcat gccaaagtct tcttgaacaa atgtacttct tcttctgtca ggcttttcaa
     8101 aatacgtttt acatataatg ttcgttgatc tgacgggatt gctggtaaaa cgatcccgag
     8161 gtctaaatct tcaaaaaagc ggatctcgtt cacagattga tggaaagcta tccattcgca
     8221 aacttgtttg gcatgaagga aagattgcgg tgtctcttgc tctgtataac aaagcgaccc
     8281 aattccaaag tttagctgta actgatacat tttattaaac tttgtctgga ttttatcaag
     8341 caatgatagc aatgaggctc tggaaatccg attgactagg ataatgattt gttgtctatg
     8401 tacagtgagc aaagcctgtc cgtaataaga gaagaccctt tcgagcaact ccaataaagg
     8461 attgatccgt tcttgacaca attgctgatt gtcagaaagt tgtcccacaa ctacataata
     8521 tggagaagaa aaatcaagct caattaagtg agggtggtat cgactatgta cttgatatca
     8581 tcaatgaacc gatggtactt tcttatgcga tggaacaggt agagcgattg cttgtttgtg
     8641 caggaaaaca agcgatgcgg ctgatacgat taggtgagaa aataagaaga taaaaataca
     8701 cggtcccttc atggcgaccg tgcatttttg gtttttatta aatatttaat actttattca
     8761 agaaatcttt tgtccgttca ttttgcggat tttcaaagat ttgttgaggc gttccatctt
     8821 ctaaaaagtt tccgtcgtca atgaacatga cgcgattggc cacttcttta gcgaacccca
     8881 tttcgtgcgt aacgataacc atcgtcatac cttgttctgc taactttttc atgacgttca
     8941 agacatcacc gaccatttca ggatcgagtg cagaagtagg ctcatcaaaa agcatgatgt
     9001 cagggttcat cgctagagca cgagcaatag ccacacgctg tttttgtcca ccagaaagca
     9061 tttcaggata catatctttt ttatcttcta gaccaactgt ctgcagtaat ttgattgctt
     9121 ttttctctgc ttcttctcta gattctttct tcaggtccat cggtgctaaa atgatgtttt
     9181 ccaaaataga aagatgaggg aataggttaa agtgttggaa taccattccg atatgttgtc
     9241 tcaccaagtt gatatctgtg cttttatcca tcaagtgggc gccgtcaata ataatttcac
     9301 cgtcagaagg ttcttctaaa cgattcaagc aacgcaagaa tgtacttttt cctgaacctg
     9361 aagggccaat gatacagacg acatctcctt cgttgatgga tacattgatg tcgtttaata
     9421 ctgtgttatc gccgtatttt ttgactaaat gctcaactaa gattttttct gccatcttac
     9481 ttcaccttct tttctaaaac ttttgctaat tttgtcaatg ctgtgatcaa aatcaagtac
     9541 atgatcgcaa tgatcaagta aacatacgta ctttgtgttg tacgtgcaac aatgatctta
     9601 ccagtttgca gcaattcgac tacaccgatt gcggaaatga tcgttgtgtc ttttaatgag
     9661 ataacgaact gattgacgaa tgatgggatc atgatgcgta tcgcttgcgg caaaatgatt
     9721 ttttgcattg tccgattata agaaagacct aaacttctgg aagcttccat ttgtccgaca
     9781 ggaacggcgt tgatcccgcc tcgaacaatc tctgcgatat aagcacttgc gttaagagtc
     9841 aaggtgataa caccagcaag gaagtcagga atcgtaaatc caacgatacc aggcaaccca
     9901 aagaaaataa agaacgcaag aaccatcatt ggaattcctc ggatgatatc gacataaata
     9961 ctagcaattg tgcgaagagc gcgtattggt gctacactga ataatccaaa aatcacaccg
    10021 attactatag ccaaagcaaa ggagatcaaa gttaggagaa ttgttctcca aagtcctttc
    10081 aacaatgctt gatagttgtt ttggataagt ccaagaaatg ttgattcatc agcagtggaa
    10141 gactcactgg aagtttctga accatccgct acatattctt taataatctt atcgtattcg
    10201 cctgttcttt ttaattcttt caatccttca ttgaacatat taagaagctc aggattttgt
    10261 ccttttttaa cggcaaatgc atattttccg ccagattctt ttttgatcgg tgtttccaat
    10321 tcttgtcctt gtttgatcgc ataaccgatt accggataat catccatcat tgcatcgatt
    10381 gcgccaactt ttaacccgtc gtacaattga tcagcagcat caaaatattt gatagaatag
    10441 ccatattcag attcatggtc gcttaaaaat tcagcacttt cggtaccaac ttttgctccg
    10501 actgtttttc ctttcaaatc ttcataagac ttgatcgaag tatctccttt tttgacagct
    10561 aattggatcc cgctatcaaa gtaagaatca gaaaaatcat agcttttctt tcgttcatct
    10621 gttacagtca ttccggcaat cattccgtct gcttgtcctg attccaaagc ttgcaaagca
    10681 gcagagaaac cacggtagtc cattcggatg ttaaatcctt gcagtttggc gatgttttcc
    10741 atcaatttta catcgatgcc ttcgtactcg ccctttgcgt tttgaaattc aaatggagca
    10801 aaagcagagt cactggcaat tacgtatacg tcttttttgg gctctatttt ttgcattgtc
    10861 tcgcttgtgc ttgactctgc cgttgtagtg ttgtcagctt gtgcaacatg accaaacgca
    10921 agtgtcacaa gtcctgtcat taagaaaaca aatgagtga
//
LOCUS       DAYYHX010000090        10867 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000090 DAYYHX010000000
VERSION     DAYYHX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.091"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            184..1443
                     /gene="lysA"
                     /locus_tag="ACHA6Y_001686"
     CDS             184..1443
                     /gene="lysA"
                     /locus_tag="ACHA6Y_001686"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM8987780.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1430..2230
                     /gene="dapF"
                     /locus_tag="ACHA6Y_001687"
     CDS             1430..2230
                     /gene="dapF"
                     /locus_tag="ACHA6Y_001687"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM8987781.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2233..3114
                     /gene="dapA"
                     /locus_tag="ACHA6Y_001688"
     CDS             2233..3114
                     /gene="dapA"
                     /locus_tag="ACHA6Y_001688"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM8987782.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3111..3827
                     /gene="dapB"
                     /locus_tag="ACHA6Y_001689"
     CDS             3111..3827
                     /gene="dapB"
                     /locus_tag="ACHA6Y_001689"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM8987783.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3833..4528
                     /gene="dapD"
                     /locus_tag="ACHA6Y_001690"
     CDS             3833..4528
                     /gene="dapD"
                     /locus_tag="ACHA6Y_001690"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM8987784.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4544..5545
                     /locus_tag="ACHA6Y_001691"
     CDS             4544..5545
                     /locus_tag="ACHA6Y_001691"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM8987785.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5542..6903
                     /locus_tag="ACHA6Y_001692"
     CDS             5542..6903
                     /locus_tag="ACHA6Y_001692"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM8987786.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6863..8014
                     /locus_tag="ACHA6Y_001693"
     CDS             6863..8014
                     /locus_tag="ACHA6Y_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8987787.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7989..9158
                     /locus_tag="ACHA6Y_001694"
     CDS             7989..9158
                     /locus_tag="ACHA6Y_001694"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8987788.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9333..10712
                     /locus_tag="ACHA6Y_001695"
     CDS             9333..10712
                     /locus_tag="ACHA6Y_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8987789.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3608 a         1897 c         2427 g         2935 t
ORIGIN      
        1 tagcttctaa tagttcgggt attttgtttt tataaccatt tttattctta tttttaaaaa
       61 aatatttcga aatatactag acaattcaga aaattttgtt taatattatt ccaataaaat
      121 tcaaatcttt atcaaatgaa attccaataa aaacggaaaa aaagtgaaaa gaggaatgaa
      181 aaaatgacag aattgataat tggcggagtc acagcaagca cattggcaga aaagtataag
      241 acacctctgt atgtgtatga tgaagaaaag atgagacaaa cgatgctgtc attcaagcga
      301 ggctttgtat cgaaacgttt tgatacaaaa gtgctttacg cctctaaagc attccaaacg
      361 attgagatgc tgcatttagt tcgtgaatat ggattcgggc tagatgttgt cagtggtggg
      421 gaaatctata ccgctttgca agctgatttt cctagagata ctatctactt ccatggtaac
      481 aacaagacgc cagaggaact gatctatgca cttgaaaatg atttgttgca tattgttgcc
      541 gataatttga tggaagttga attattagca gaattaagcc aacgctattt aaaaaaaatc
      601 tgtatcatgc ttcggttgaa tgtcggagta gaagcacata ctcatgaata tatcgtcact
      661 gcgcatattg attcaaaatt cggcatgagc tatgaaagta aagagtgcca acaagccctt
      721 cagttgataa aagaaagcag ctatctagag ttagaaggat ttcatgctca tatcgggtca
      781 caaatttttg atgttactgc ttggttagct gaaatcgaca aattgacgaa ttacttaacc
      841 gattttgacc aacctctttc cttgaatctt ggcggagggt ttgggattcg ttatacacaa
      901 aatgatcagc cgatcccagt cgaagaggca ctaaaaaaac tgattgaata tacggaagaa
      961 gctttggaaa aacgaaaact gaccattcgt caattactga tcgaaccggg tagaagtatt
     1021 gttggagaag ctggtacaac actatacacg attggtttca tcaaaaagac accacataaa
     1081 aaatattact ttgtagatgg cgggatgaca gataatatcc gtccagctct gtatcaggca
     1141 gaatacgatt gtgatttagc agcaaaactg tcggaagaaa aaacagagaa agtgacgatt
     1201 gcaggaaaaa tgtgtgaatc aggcgatgtg gtaattaaag aagcttattt gccgaaagca
     1261 gaatcgggtg atcttttagc tgtttattcc acgggagctt atggatactc gatgagcagc
     1321 aactacaatc gagcgacacg accagcagtt gtttttgtca ataatggcca atcaagactg
     1381 attgttcgca gacaagcatt ttctgatcta ctaagggggg aagtttccta tgatccttca
     1441 taaatataat ggctgcggaa atgattttat cttattagat tacgcagaag acacagatta
     1501 tagtcaatta gccgcttctt tatgcacaaa agaaaaatat gatatagacg gattgatcgc
     1561 cgtaaaaatc gatccgttgg aaatgatcta ttacaacaaa gatggcagcc gcgcgccgat
     1621 gtgcggaaat ggcattcgct gttttgctcg atacgtaaat gatcagaaca tgatgaatga
     1681 acaggaattc aacgtagaga cattagcagg cacaagaaaa attacggtgt tggatccctc
     1741 acctttttat tgcagcgtag atatgggagt gccagatgat tctccggaaa aaatcaaagc
     1801 taatcaaact tatccaataa aaaaccagga aatagagatc aatggtgaaa aagtaacgat
     1861 cactagttta tttttaggaa cgattcatac cgttgttttt gttgaaaatg cgcgtgagga
     1921 agtctggaga aaaagaggtg aactgatctg tcatcatcct ttgttcaaag aaaaaaccaa
     1981 tgtcaacttt gtagaagtag tcaatgaaca agaattgatc gtccgtacct atgaaagagg
     2041 agtaggttgg acacttgctt gcggtacagg ttgctgtgcc tcttatgtgg ttgctaagga
     2101 aactgggaaa atttcagcag atcaggtagt tgttcacttg gaacaaggag acttgatgat
     2161 ctctggtgag gatgcgatac aaatggctgg cccagcagtt catgaatgga caagaatatt
     2221 ggaggaatga agatgataaa aggttcgatc gtggcgctga tcacgccgat gaatgaagag
     2281 ggatcagtag attatgctgg tttggaaaaa ttgatccaat tccatttaga tgaacaaaca
     2341 gatggattat tagttttagg tacaacagga gaatcttcta cgctcacaca atctgaggag
     2401 gagcagatcc ttcaactgac agtaaagaaa gtggctggcc gtgtgccggt gattgcagga
     2461 gctggaacga ataatacgaa agaaacgatt gaaaaagcga agcattttgc ttctttgggg
     2521 gcagacgcac ttttggtcat caccccttac tacaacaaaa caagtgatgc aggactagct
     2581 gctcatttta cagcaattgc agaagcctca ccgattccgc ttattttgta taatgttcct
     2641 tctagaacag gcatgtcgat tccaatccat gtattagtaa atttagcaga acatcctaat
     2701 atcattggtt tgaaagaagc ctcaggagat atggcttatg tgatggatgc cgctcggttg
     2761 attggagaag aattcttcct ttacagtgga aatgatgatt tgatcctacc agtaatgagt
     2821 gtaggaggaa gcggtgtaat aagtgtatgg gcgaatatcc aacctaaaat agtccatgaa
     2881 ttagtaaaag atacgcagga cggaagatgg cagcaagcaa aagaaaagca attaaatgca
     2941 cttgaactga tccatgcctt gttcagtgag accaatccta ttcctgtcaa agcagccatg
     3001 tcattattag acttaccttc cggacctcta cgcctgcctt tagtttctct ttcaaaagag
     3061 aaaaagaaac aattagctca gcttttgtta aaagaaagga cggttgaaaa atgaaaatcg
     3121 gactgattgg cagtggacag atgggaaata gagtcgaaga agttgcacaa gaaagaggag
     3181 atacggtcct tcttttacgg acggcaccta aagaagcaac tacgcttgct tttacggaac
     3241 tgccagaact gcttattgat ttttctcatc cagataattt agagaaaatc ctttcgtata
     3301 gcttatcata tcaacttccg ttagtcattg gcacgacagg ttatacacaa tcacagttcc
     3361 aagaaataaa agaacatgcc aaacatgtac ctgtgatgta tagtgctaat ttttcgttcg
     3421 gcatcatggt gatgaatcaa ctaatcaagc aagcagctgc aatgttgcaa ggctggcaga
     3481 tcgaactgat tgaaaaacat catagtcgta aaaaagatgc accatctggt actgcaaata
     3541 tgctgcttca aacaatccaa gaagtgcaaa agctgcagcc cgtatacaac tggcaaggga
     3601 aaaaaagaga agaaaaccaa attggtgtac acagtattcg tgcaggttct ttacctggtg
     3661 aacatgatgt attgtttgct gctactgatg aaatatttac gatcaaacat gaatcttttt
     3721 ctaatcgaat ctttgctgca ggcgcagtag aagcagcgga ctggttgaaa gatcagtcac
     3781 caggttatta caaattagaa gatttattaa tggacaaagg ggtgtaaaaa acgtggaagc
     3841 tcaagaaatc atccgctaca ttcaaacggc atctaaaaag acgcctgtga aagtctatct
     3901 gaatgtaacc gagccaatcg aatttaaaga aagtaaagtt tttggtgaag gaagcagctt
     3961 cgttgttttt ggtgactatg agacgattgc tccggtttta gaagctgaat cagaaaaaat
     4021 cattgaaatc gaaatcgaaa ctgctgcaag atacagcgca gttcctttgc tagatattaa
     4081 aaaaattaat gctcgtatcg aaccaggtgc tattatcaga gatcaagtgt caatcggcaa
     4141 caatgccgtg atcatgatgg gagcgattat caatattggt gcagtaatcg gtgaaaacac
     4201 gatgattgat atgggtgctg tcttaggtgg acgagcgaca gtgggcaaaa actgccatat
     4261 tggtgcagga gctgtcttag caggtgtgat cgaaccagca tcggccaaac cggttatcgt
     4321 agaagacggc gtactggtag gtgcgaatgc agtgattgtt gaaggtgtcc atatcggaaa
     4381 agatgcagtc gtggcagcag gagcagtggt tttagaagat gtggcagcag aaactgttgt
     4441 cggtggaata cctgcacgtg tattaaaaat aagtgatgat cagacaaaag aaaatacagc
     4501 attgattgct gcgttacgcg aattatagag gagatggaga aaaatgcgcg aaatagatac
     4561 ctgtattgtg ggggcaaccg gattagtcgg ccaaacgatg ctcaaagtgt tagaagaata
     4621 tgattttcca gtaggacgat tgaaattatt agcttcaaaa cgttcagccg gtaaagtttg
     4681 tgtattcaag aaaaagaaac acactgtaca ggaattgaca gaaacttcat ttgaaggata
     4741 tgatttagcg ctattttctg caggtgcttc tgtatccaaa gagtttgcgc caatcgcacg
     4801 aagtcaggga ctatttgtca tagataattc ctcagcatgg cgggaagatt cggatattcc
     4861 attaatcgtt ccagaggtca atctggaaga ctatacattg aatcggctga ttgcaaatcc
     4921 aaattgttca accattcaag cggtacttcc gcttaaagct ttacaagaag cctttggtat
     4981 ccagcgagtc aattattcga cctatcaagc agtttctggt tccgggcaaa aaggcattga
     5041 agatcttcat cgtacgcaaa atgacgagga accagttttg tatccttata atattagcca
     5101 aacggtgatt ccggaaatcg atcagccttt ggaaaatggg tatacaaaag aagaacagaa
     5161 aatgataaat gagacccgta agattctgca tcagcctgct ttgccagtat cagcaacctg
     5221 cgtacgtgtg ccaatcgaaa atggacatgg agtctctgtg gcagttcaac tgcaaaaaga
     5281 gtttacgacg gaagaaatta gagagtgtct cagccagttt ccagggattc aattagaaga
     5341 tgatctagca gcacataaat atccgacgag cattcttgct agaggaacag atatggttta
     5401 tgttgggagg atcagaaaag acacttcttt agagaacgga ttgttgttct atacgactgc
     5461 cgataacata cgcaaaggag ccgctgcgaa tgccgtacag attgctgcag ctttaatgca
     5521 gaaaatagcg gaggtggcaa gatgagtaat acgctgaaag tagcgaaatt cggcggaaca
     5581 tcgatgtctc atgaagagca attcaaaaaa gtccgtacaa ttgttctttc agatcccaat
     5641 cgccaagtga tcgttgtttc tgctttaggc aaacgtacgt cagatgatga caaagtaaca
     5701 gacttacttt atttgattta tgcttacctt cagcatcatg ttgattggac acccctttgg
     5761 aaaaggattt gtgatcgttt cattcaagta agagatgttc ttgccctgca gttacctatt
     5821 gaagatgaac tagcacgcat acagaaacaa ctggaagacg aaaagataac tgaagattat
     5881 ttagtaagta gaggtgaata tctgactgct caactgatgg ctgagtatct tggttaccag
     5941 tttattgacg caaaagacct gattttcttt gactatgatg ggaaaatcga tcaggaaaaa
     6001 acagcacagg aaatcacaaa agcatttaaa agatatccga aaatggttgt tccagggttt
     6061 tacggggcga accctgcagg aaaagaaaaa ttattgggcc gtgggggaag cgacattacc
     6121 ggcgcgatac tggcgaagat gttgcaagcg gaaagttatg agaattggac ggatgtttca
     6181 ggaatcatga tggctgatcc gcgaatcatc gatcatccta aaatcatcga ggaaatctct
     6241 tttagagaac tgagagagat ggcttatatg ggggctaacg ttcttcatga agcagctgtt
     6301 ttcccagttc aagaggctga tatccccatc caaataaaaa ataccaatga tcctttcgct
     6361 caagggacaa aaatatcgaa tcacgaaata gaaaaggaaa atggactgac aggaatagct
     6421 ggacgcaagg attttctttc catcacttta ttcaaacgtc atatgtccga tgaaatcggg
     6481 tttatctgga aagcaatgag catttttgct aaacatgaga tatcaatcga acatattcct
     6541 tcagggatcg acaatatcgg agtgatcgtc tcagcagaag cgattgccga taagttattt
     6601 ctcatcacga aagaattaaa agaaacactt ggcgtggaag aaatcgtcgt gatcgaagac
     6661 ttagctttga tctctgtcgt tgggggtccg cataaagaat tgattggatt atctgggaaa
     6721 gtgttatcga tattgaacaa attagaaatt cgcacatcga tcttatcaca aggagcacag
     6781 gagttgaatc tgatcatcgg tgtaccgaat aatcaatatg aaacagtagt gaagggtatc
     6841 tatgaaggga tggtgaatac ggatgctgaa agaatatcga atggcactcc atcaaattcc
     6901 tgaaatcggg tttcatgaat tcaagacaaa aaaatattta tatgaacaag taaaaaacag
     6961 cgaggccgtt atccatgaaa tcggcgaaac aggactactc ctttattttg ataatcagca
     7021 agaagaaacg attgcttttc gaacagatat cgatgcactg ccagtcaaag aagaaacgaa
     7081 tctctctttt gcttctaagc atgaaggtta tatgcatgca tgtggtcatg acggacatat
     7141 ggcgatgctt ttagggttga ctgactttat aactaaacac attgaggatc ttatttataa
     7201 tgtagtactg atctatcagc catcagaaga aatttccggt ggcgcacaaa gcgttattca
     7261 gtcaggattg ttggataaat atgaagtaca agcaatattt ggctttcatc tatggcctgg
     7321 actagctaag ggagaagttt tctctcgacc aggacctttg atggcacaaa gcagtgaaac
     7381 agacattatc attaaaggcc agtctgcaca tattgcttct agtgaaaaag gaatcgatag
     7441 tctggaggca gctgtacgat ttttgaagaa tgtttatgat tttgaccggc aattaccggc
     7501 agagtggaat catctaatga aatttggcca aatccaaggt ggaacgatcc gaaatgtttt
     7561 agctggagaa gttattgtat ccggaagtat ccgttcttac agttcagata tgcaaaatta
     7621 tttgaagagg ggattagacc atttagcaga acagtttcac tctacagcta ctatttcttt
     7681 tcgctataat gacggctatc cagctgtaat caaccataga ggcttatatg cagcactgtt
     7741 agattcaaac aaactacatg aattagatgc tccagttctt caagccgaag attttggcat
     7801 atatacgcaa caatatccat gtgtattttt ctttttaggt gtcggtgata cccctgcttt
     7861 acatgatccg acgttcgatt ttgatatgga tgttttagaa aatggtgtag aatggtacaa
     7921 aacgattctt tttacagaaa atcttcttgt aaaaatcgat agcagttcaa aagaggagcg
     7981 agtaaataat gatgaattta tccaatcgat ttagtcctag gctttctaga atagaagtat
     8041 caaaaatacg acaattcgat cagcaaatat cgtcgatacc agatgtgatc aaactcaccc
     8101 tgggagaacc ggattttcca actcctgaac atgtcaagca agcaggaata gcagcgattg
     8161 aagaagactt ttctcactat accggaatga gaggattaga ggaactgcgg gaagcggctt
     8221 gtatatttca gcaacagcgt tatggattaa cttatgatcc gcaaacggaa gttttgacga
     8281 cagttggtgc aacggaagcg attgcctctg cgttgttatc tgttttggaa gaaggagaca
     8341 aggttcttat tccagcgcct gcttattcag gatatcagcc attagtggaa ctagctggtg
     8401 ctgaattgat cccgatagat acttctgaca cagggtttgt ctgtcagccg gaacaatttg
     8461 aacgagcatt tgaacaatat ggatcagctg tcaaagcagt cattttaaac tatcctaaca
     8521 atcctactgg gacgacattg tctgcaaaac aattgaaagc aatcgctgag gttttaaaaa
     8581 aatatccggt atttgttata agtgatgaag tatatgctga attgacttat agtggaacgc
     8641 atatgtccat agctacttat cttccagaac aaacaatcgt gataagcggg ttatccaagt
     8701 cacatgcgat gactggttgg cgagtgggct ttatttttgc tcaaaaagcc cttattgacg
     8761 aactaattaa agtccatcaa tatctggtga ctgccgctac aacgatgtcg caaaaagctg
     8821 cggctgaagc gctaataaat ggggtgaaag atagcgagaa tatgaaagag caatatcaaa
     8881 cacgaagaga ctatctggtc cagcaattgg cgcctttagg atttgaagtg acccagccaa
     8941 acggtgcatt ttacttgttt tgcaaactcc cagctgccat tcaaactgac tcttggcagt
     9001 tttgtgtaga attagcggaa caggcaaaag ttgcgtgtat cccaggaatt gcatttggcc
     9061 cagaaggaga aggttatatc cggatttctt atgctagcag catggaaaaa ttacatgagg
     9121 cctgcaaacg gatcaaagac tttttaacag aacagtgatt catagatttt atcaaaaaaa
     9181 caatcgttaa ttttgcggta tattctgcaa ggagacgatt gttttttaaa tttagttcaa
     9241 atgaacgcgt ttcttttgat tcataactaa tgtaatgtta agtttaaatt gtttagaatg
     9301 attttaaaag aatattcaag gaggtttcat caatggaaaa aatcaaagtt gtcattgtag
     9361 gtgcatctca tggagggcat caatcgatct tggaactatt gacacgatac ggtgaaaatg
     9421 tagatatcac gctatttgaa gcaggagact atatttcctt catgtcatgt ggtatggagc
     9481 tgtatttaga agacaaggta acagatgtaa atgatgtacg taatttcaga ccagaaaact
     9541 ttccgcaatc caatgttcat attttaaata atcacgaagt aaaaacgatc gatgccgata
     9601 aaaaaacagt aacggttact agcgcatcag ataacgaaac aaaagaatat ccttatgaca
     9661 aattgatttt gagttctggt gtcaaaccaa acaatctgcc agtaccagga gcagatctag
     9721 aaaatgtcta cttgatgcgt ggatacaatt gggcaaccaa aatcaaagaa aaattaaatg
     9781 atccggctat taaaaaaatc gctgtcgtag gtgcgggtta cattgggatc gaagcagcag
     9841 aagcaagccg aaaagctggg aaagaagtcg tattgcttga tgtgatcgat agacctttag
     9901 gcacttattt agatgctgag atgacagata ttttagaaca acacctaaat gaaaacggta
     9961 ttgaagtttt gacaaatgca aaaatcgaag cttttactgg taacggaaaa gttgaggcaa
    10021 tcaaaacaag tgaaaaagaa atcccagcag atctagtcat tcaagctgcc ggtgtgaagg
    10081 caaatacaga atggttaaaa gggatcgtcg acttagatga acgtggctgg atccaaacaa
    10141 atgaatactt gcaaacgaat cttccagatg tgtatgcagt aggagatgct acacttgctt
    10201 attctatccc agcacgcaaa aaaatgccga tcgctttagc gactgttgca cgtagagaag
    10261 cacgttatgt agtaaaacat ctttttgaag aagtaccaaa caaaccgttc ggcggagtcg
    10321 taggttcctc tgcattaagt gtctttgact atcattttgc agcaagcgga ttgaatagct
    10381 ttacagcagc aaaagcagga gtagatgtac gagtgtctta ttatgaagat acgttacgac
    10441 caaaatatgt accagaagaa caaggaaatc caaaagtgtc catccaatta ttctcggatc
    10501 caatgaccca tcaattgcta ggaggagcag tattatctac ctatgatgtt acaggtcaag
    10561 gaaatgtatt ggcactagcg atccaaaaca aactgacttt agaagaacta gcagaagctg
    10621 acttcttctt ccagccaggc tttgaccgac aatggagcat gttgaatcta gccgctcaac
    10681 aagcgttagg agaagaacca tttgtagaat aagaaaacga aatatttgaa aagaggttgt
    10741 gaaaaagctg ccttgcatca agtaataaga acagccaagt aaaaacagct tcttatgttt
    10801 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attttcaaaa
    10861 aagggac
//
LOCUS       DAYYHX010000091        10707 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000091 DAYYHX010000000
VERSION     DAYYHX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.092"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..645)
                     /locus_tag="ACHA6Y_001696"
     CDS             complement(<1..645)
                     /locus_tag="ACHA6Y_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8987790.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKF"
     gene            complement(658..2160)
                     /locus_tag="ACHA6Y_001697"
     CDS             complement(658..2160)
                     /locus_tag="ACHA6Y_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8987791.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            2708..4387
                     /locus_tag="ACHA6Y_001698"
     CDS             2708..4387
                     /locus_tag="ACHA6Y_001698"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8987792.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            4514..4993
                     /locus_tag="ACHA6Y_001699"
     CDS             4514..4993
                     /locus_tag="ACHA6Y_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM8987793.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            5212..5892
                     /locus_tag="ACHA6Y_001700"
     CDS             5212..5892
                     /locus_tag="ACHA6Y_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM8987794.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            5900..7231
                     /locus_tag="ACHA6Y_001701"
     CDS             5900..7231
                     /locus_tag="ACHA6Y_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM8987795.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            7246..8208
                     /locus_tag="ACHA6Y_001702"
     CDS             7246..8208
                     /locus_tag="ACHA6Y_001702"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM8987796.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            8319..8657
                     /locus_tag="ACHA6Y_001703"
     CDS             8319..8657
                     /locus_tag="ACHA6Y_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM8987797.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            8736..9443
                     /locus_tag="ACHA6Y_001704"
     CDS             8736..9443
                     /locus_tag="ACHA6Y_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM8987798.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(9546..>10707)
                     /locus_tag="ACHA6Y_001705"
     CDS             complement(9546..>10707)
                     /locus_tag="ACHA6Y_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8987799.1"
                     /translation="AVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCT
                     LEGNELSIRLYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLL
                     TLFILRNKQQAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNY
                     FFYCFFPNQISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFY
                     FLVLAVVFFELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQI
                     SQYLSQLIQHLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHA
                     DLIITDQHEKISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         3409 a         1886 c         2265 g         3147 t
ORIGIN      
        1 aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt caatctcatg
       61 agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa gtttgacgat
      121 atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga ttagttgatg
      181 ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg aaagtttggt
      241 cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat ctaaagcaac
      301 ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga cgttgtctag
      361 agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat catttttacg
      421 ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct ttccttcttt
      481 tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac cacttgctcc
      541 taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat aaagcgcatc
      601 ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat tcctccttta
      661 gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta aaaagacaga
      721 aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag gtttgacatt
      781 gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg ttccttgttg
      841 accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac cgccaggagc
      901 ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc ccacggcatt
      961 cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac aaatagccac
     1021 ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata gcaagacgcc
     1081 taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta tgataatacc
     1141 ggctacagcc actagtaaaa caacattttt agaaaaactt gccacgttgt ttaaaggttc
     1201 caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg tgtcgatcag
     1261 cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag tgtaaatagc
     1321 agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag tgtaagaagt
     1381 gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc cgcttgtttc
     1441 gctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat cttcagtact
     1501 cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag tagaatcaat
     1561 gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg tgatttttgc
     1621 tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga tttgaaagtc
     1681 tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc ctgcatcagt
     1741 agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc catcttttgc
     1801 catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt cagctatttt
     1861 ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg gacgttcttc
     1921 agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt ctcgattggc
     1981 acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt cttctgcagc
     2041 gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca cggcaaacaa
     2101 taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca taaaattcat
     2161 attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta aagctagctt
     2221 aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa aagtcactat
     2281 agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac gaagaagaaa
     2341 tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac gtgcatagtt
     2401 gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa tggggaacag
     2461 ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat tttgttaatg
     2521 aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc tggtaatttt
     2581 cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat tggaatggct
     2641 catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat aagaagaggt
     2701 gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa atggaaaaaa
     2761 catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc taaaatatcc
     2821 tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt tagaagaaaa
     2881 cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga tcggggcact
     2941 tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga cgatcgagtt
     3001 agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt tccatgtggt
     3061 agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc gtaccactca
     3121 tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta tcgtttatac
     3181 gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt atgcacgatt
     3241 ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa atgcagaaaa
     3301 tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata cgatcaaata
     3361 ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag tgcaacaagt
     3421 attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag atttcgaacg
     3481 aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag atctgcttgt
     3541 tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag aaactggccg
     3601 aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc gtaatgtcaa
     3661 gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga tggaaaccat
     3721 cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt tgatctctga
     3781 gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga tcaatttgat
     3841 gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg ctcatgcaga
     3901 tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac gcggagacgt
     3961 gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag aaaatgtaat
     4021 gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc gaaaaatctt
     4081 gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa agaaaatcat
     4141 ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc gtgatctgat
     4201 gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa gcaatgattt
     4261 tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact tatttgaaca
     4321 aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac gtcgaaataa
     4381 aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg ctcttttttt
     4441 gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac gatagaataa
     4501 agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag aacaaaagat
     4561 cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag aagaactaca
     4621 aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag gaaacaaaac
     4681 tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa aaacgagaca
     4741 agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa tgcatacagg
     4801 atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg attattgttt
     4861 tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg gtgaaatcgg
     4921 gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta tttacgctga
     4981 tttaattagt taagggacga gaagagaagc taagcagagc agagacctca aacttgagat
     5041 tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt tgtacatact
     5101 gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga tgtgcaagtg
     5161 atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa tatgaaaatt
     5221 acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg cgtgatgatt
     5281 gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt aaagacagat
     5341 tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc tattgcaaaa
     5401 gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa cagcagagga
     5461 gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg cgtggtaaaa
     5521 ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca gtatatgggc
     5581 gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc aggggatact
     5641 gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt cgctttcttg
     5701 ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc gcaaacaatt
     5761 cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga acaagatccc
     5821 gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt ctcggaaacg
     5881 ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat atattgaaag
     5941 tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga atgtcagtga
     6001 aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt caacaattca
     6061 acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac gtttgacatt
     6121 tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag gattagacaa
     6181 ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac gctatattac
     6241 gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag cattggaaga
     6301 tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg caaagctagc
     6361 tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag tcgcaaccat
     6421 gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg tcagtgatat
     6481 ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt ctgattatca
     6541 gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc atcatcgttt
     6601 gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga tcatcgatcg
     6661 ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct ctgttagcca
     6721 tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact tgacattgga
     6781 aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc gacaaccgca
     6841 gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg atactgaccg
     6901 tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac aaatggtcaa
     6961 tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg tcgcacaaaa
     7021 aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc tacattattt
     7081 gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg aaatcggcag
     7141 acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt ccaaagcaat
     7201 cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac agtacagcaa
     7261 gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca tttgcgccct
     7321 gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga aagtttccca
     7381 gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt agcagaaatg
     7441 caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga tacagctaat
     7501 tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa aatcgaccat
     7561 catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc aagtagctgt
     7621 agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat gaacgttaag
     7681 gctgcacggt tactttatgc aggaatcgtc ggagatacag ggcgcttttt gtatccctcc
     7741 acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt tgatgcaagt
     7801 gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc cggttatctt
     7861 tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc gcaagaaatt
     7921 cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact tcctggagtg
     7981 attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta ttaccgcgta
     8041 cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca tggaggaggc
     8101 catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga aatctataaa
     8161 gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata cttcgttcga
     8221 aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc tttttccgtt
     8281 acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca aattgcccag
     8341 aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca gaatgcggga
     8401 acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg gtattagatg
     8461 caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac ttgaaagtaa
     8521 aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt ttagtagaag
     8581 gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg aaatcggaat
     8641 ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct actactaatg
     8701 ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga atgtatggtt
     8761 ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga attgcgtcag
     8821 cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac aatgtgactg
     8881 gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc ggtgttcaac
     8941 aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg agtttgtctc
     9001 aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat gaatgcggac
     9061 atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca cttgttccag
     9121 cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta ttgcttagct
     9181 ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta cttttccaat
     9241 tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt ttgagtgaag
     9301 gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt gcagcagcac
     9361 tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg atcttattgt
     9421 tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt tgatctgagc
     9481 taataccgag agttagctga ttgaatacca tttatttgga ataagaggat gtgacaagaa
     9541 ttttgtcaca tcctctttgt tttgttctca taaccttttt tcatatacag ttcatgaacc
     9601 gcttttatta acttgccaaa taacactgta ttgtgcgttg tatccagata gaaagtctcc
     9661 gtttcatctg aaattttctc atgctgatct gtaatgatca aatcagcatg ttctgaatat
     9721 gcggtaattt taacagcatc tttgcgaaat aactgctgca aatggctttg aagatgataa
     9781 tttcctgtaa actgtttggt tatctgcaaa tggatgagta actgctcttt cgtttctgtt
     9841 tgtagtaagt gttggatcaa ttgactaagg tattgactga tttggtttct ttccccattt
     9901 tttgaaaaaa ttgtatcgat tttttctctt aatttctttt cctgtataaa agaactagaa
     9961 tgattggacg tggcttctgg aaagcaaagt gtagaaaaac tactgctgga ttcacccaac
    10021 agttcgaaaa aaacaactgc caacacaaga aaataaaaat aaattggtat tttattttct
    10081 aaatggttga gaggaaactg ttgagttaag taagtcatca attgaccgac ttgtcgacaa
    10141 ataaggtttt tgcttgtgga aaataaaaaa ccaatctcct gtttttgtcg aaaagagatt
    10201 tgatttggaa aaaaacaata gaaaaaataa ttaaagtagg ctctctctgt ttctgtctct
    10261 tcacaagggc gactcactga aaaagaagta aagtattctg aataatcatg tgtatgctgc
    10321 aataagtcca tcaattcagt gatttcagaa gaagtttttg gaaaatatgc ctgttgtttg
    10381 tttcgtaata tgaataaagt taacagcaag tagatatttt tttcttgttt tgaaattttt
    10441 tctatttgct catctgtact gttcattttt agtgcattta attcaagtga ggaaaatggc
    10501 caatcgttac ctccaaatgt atctgaaaga aaaaagtaaa gatagagacg gatagataac
    10561 tcatttcctt caagagtaca gcaatttttt acagatagga tccgtaaatg gaattttatt
    10621 aaggaatgat tgagctgttt tgtcagtttt tcgagatagg agggcgatat ataaagttgt
    10681 tcaagtaaat atttttttgg tacagca
//
LOCUS       DAYYHX010000092        10670 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000092 DAYYHX010000000
VERSION     DAYYHX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.093"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(188..1036)
                     /locus_tag="ACHA6Y_001706"
     CDS             complement(188..1036)
                     /locus_tag="ACHA6Y_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8987800.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1200..2102
                     /gene="gnd"
                     /locus_tag="ACHA6Y_001707"
     CDS             1200..2102
                     /gene="gnd"
                     /locus_tag="ACHA6Y_001707"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM8987801.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2104..3105
                     /locus_tag="ACHA6Y_001708"
     CDS             2104..3105
                     /locus_tag="ACHA6Y_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM8987802.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3102..3386
                     /locus_tag="ACHA6Y_001709"
     CDS             3102..3386
                     /locus_tag="ACHA6Y_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM8987803.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3419..4216
                     /locus_tag="ACHA6Y_001710"
     CDS             3419..4216
                     /locus_tag="ACHA6Y_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8987804.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4230..5063
                     /locus_tag="ACHA6Y_001711"
     CDS             4230..5063
                     /locus_tag="ACHA6Y_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8987805.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5081..5938
                     /locus_tag="ACHA6Y_001712"
     CDS             5081..5938
                     /locus_tag="ACHA6Y_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8987806.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5960..6469
                     /locus_tag="ACHA6Y_001713"
     CDS             5960..6469
                     /locus_tag="ACHA6Y_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8987807.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6506..6907
                     /locus_tag="ACHA6Y_001714"
     CDS             6506..6907
                     /locus_tag="ACHA6Y_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987808.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7019..7372
                     /locus_tag="ACHA6Y_001715"
     CDS             7019..7372
                     /locus_tag="ACHA6Y_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987809.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7406..7849
                     /locus_tag="ACHA6Y_001716"
     CDS             7406..7849
                     /locus_tag="ACHA6Y_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8987810.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7866..8951
                     /gene="uxuA"
                     /locus_tag="ACHA6Y_001717"
     CDS             7866..8951
                     /gene="uxuA"
                     /locus_tag="ACHA6Y_001717"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8987811.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8944..9657
                     /locus_tag="ACHA6Y_001718"
     CDS             8944..9657
                     /locus_tag="ACHA6Y_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8987812.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9864..10013)
                     /locus_tag="ACHA6Y_001719"
     CDS             complement(9864..10013)
                     /locus_tag="ACHA6Y_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987813.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10409..10609
                     /locus_tag="ACHA6Y_001720"
     CDS             10409..10609
                     /locus_tag="ACHA6Y_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8987814.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3560 a         1690 c         2315 g         3105 t
ORIGIN      
        1 gttatcgcat ctaacaagta actcgaataa taccaataca accttctcca aagatgcgat
       61 gacatacgtt ctaacccacc gactattgat gttctgtacc acccttgcag aaccaaaaaa
      121 gccactacca aatatttggt agtggctttt tttgttttat ggatttttcg ctttttctgt
      181 tatggtgcta ttctttgatt tcagaaatag tatgtcgaac tttagcgata gaacgacgtg
      241 attctttttc gttgtaaaac atcaaaatca catatagaga gtccaaaatg cttagttgag
      301 aaatacggga agctaaagct tcagatcgga attctgtttc ttcagaaata gaaataaaaa
      361 cgacatcgcc aagtttagca agggaagaat tagcttggct agtaataacg attgtttttg
      421 ctccagtttt tcttacggtt tcagcaatat ggattgtttc ttttgattgc cctgtgtgag
      481 aaatcaaaac agcacagtct ttttccgtca ataaagaagc ttccatcaat tgaatatggt
      541 aatccgtgct gtgagaagtt gcgatagggg aacgcaaaaa tttatgataa gcgtcagttg
      601 ctacaatctc tgaaccgcca acaccgaaaa aatagacacg tttagattca ttgataatag
      661 tagccgcttt ttttagatcc ttttgttgta acaacttttt tgtgtcattt aaagttgaaa
      721 tactagactc gaacaccttt tgcgccatag tgagctcagt atctgttttc tgaatatttt
      781 cgtggataga aatagcagat agatcatttt cttggatcaa catggccatt ttaaaatctt
      841 taaatccatt ataaccaagt ttttttgtaa attgaaaaaa agtagagtca gcaatgccca
      901 gttcacttgc taaattgcta ataggactat gacttacttt gcttggattt tctaaaatat
      961 aatcggcgat agcctgttct tttgggctga agtcgttgta cattgttttg atttgtagtc
     1021 ttatggagac gctcatgaga attcctcatt ttcataataa gattatcatt ttttccgttc
     1081 tcattaatgt taaagataca aggagagtag gaaaaaatca aacaaaaaat atttttgtaa
     1141 aatctattta caaaaaaatt tttttgtata atgagggtat aagaaatgga ggcgctatca
     1201 tggaaattgg tgtaattggt ttgggaaaaa tgggattgaa tttagcttta aacttaaaag
     1261 agcaccatca caaagtacaa ggactcgaca tttcaagtac agctgttagt caggcaagag
     1321 aaaatgacat tcctgcttat caagagatct ctgaatttct agctactttt gagcagaaaa
     1381 aaatcatctg gttaatgctt cctgctggag agataaccga aaacatgtta agaaaacttt
     1441 ttccaaaact cggaccagga gacattatta ttgaaggcgg aaactcttat tataaagatt
     1501 caatccgtcg agcagaagag tttcagaaaa atgaaatcgg ttacttggat tgtgggactt
     1561 ctggaggaat cgaaggagca cgacatcaag cctgcttaat gattggagga gataaagaaa
     1621 cttttctatc cgtggaacaa ttattcaaag atgtagcaat tgaaagagga tatctgtatg
     1681 ctggtgcatc cggaagcgga cacttcttga aaatgatcca taatggaatc gaatatggaa
     1741 tgatgcaagc aatcggtgaa ggattccaac ttgtacaaaa aagtgagtat gcttttgact
     1801 tggaacaagt cgcacgtgtt tggaatcatg gttcggtgat tcgcggctgg ctgatggaaa
     1861 tcatggaaga tcagttaaac gaacatccca atttaagtaa ttatcgggga atcgtttctg
     1921 catcaggtga agcaaaatgg acggttgaaa cagcgctaga aatggctgtt ccagttccag
     1981 taattgcaac tagtttgttc atgaggaatc tttcacagga agaagatagt ttttcagcaa
     2041 aagtagtttc agcactaaga aatgggtttg gcggacatga aatagttgaa aagggtgagt
     2101 aaaatgccag attatcatat agcaatcgtt aattccagca gtttcggaag agtctttcca
     2161 caccacttgg aaagattaaa gtcagttgga gacgttcatt tctttactgt tgatcaagag
     2221 attccaggaa aagaactagc ggagttactt aaaggattca atatcattat tgcaagtgtt
     2281 acgccatttt ttcgacgaga tttttttgaa aacaaagatg aactgctatt gatttcccga
     2341 catggtattg gttacaacaa tattgattta aaagcagcaa aagaacacca gacaatcgta
     2401 tctatcatac ctgctttaat tgaacgaaat gctgtagcag aaaataatat aactaatttg
     2461 ttgtccatgc ttcgacgcac gacacaagca caagcaagag tcaaggacga tcgatgggaa
     2521 gatcgggcgg agttcgtcgg taggacgctg tttaacaaaa cagttggtgt gattggtgca
     2581 ggaaataccg gaagcggagt aatcgaaatt ttgcgtaacg gttttcgctg tgatgttcta
     2641 gcttatgatc cttacaaatc agatttatac atcgaaagct ttggtgcaaa aaaagtagaa
     2701 cttcaagaat tactggaaca atcagatatc atttgtttat gtgcgaattt gacagaagag
     2761 aactatcaca tgatttctga taaagaagtc gatcagatga aagaaaatgt ttatctatct
     2821 aatagtgcaa gaggcgctct agttgatgaa gaagcgatga ttaatggtct gaaaagcgga
     2881 aagatcgcag gatatgccac agatgtttta gaagaagaac cgggacgtca agatcatccg
     2941 tatttagcgt ttgaacaagt agtgattacg ccacatatat cggcctacac aagggaatgt
     3001 ttagaagcaa tgggtgaaaa atgtgtcaga gatgtcgaac agatcagtca gcaccttctt
     3061 cctgaacgtt ctgtccagac aaagagcaac tatataaaag gatgatgaag aaaatggtga
     3121 acggtttaaa agtgaaaaca ggccctcaat tttatttata tgaagaaggt ggtatttcaa
     3181 aagtttctga tctcttgaaa tcatacggcg cgaaaagagt actggtcacc cacggaacgg
     3241 tatcatggga aaaggcgcta cctaaattag tatttttgaa tgacgaaacg attcaatttt
     3301 tttatcatcg ttattctgga gaatgtagtt atgctgaagc cagacgaata gctacaatca
     3361 tcaaaaaaat gaaatcgatt tcgtagttgg cgttggcgga ggaaaactga ccgatctagt
     3421 gggttatagt gccaatcttg caagaatcga tttcggagtc atccctactt tagctagtaa
     3481 ttgtgctcca tggacacctc ttgctgtcat gtataaggag aatggacaag cagaagggaa
     3541 aacagaacac ttttacagac aagctgcttt ttttataaca gatccccaat tagtgattga
     3601 ttcccctgtt ccatatttca ttgccgggtt agcagataca ctagcaaaat ggtatgaatc
     3661 tgagctcatc ttagaacaag attttttgca agatgaatca tttttgaagc tagcacaaga
     3721 tactgcgaag atctgcaaag aagagatttt gaatcattct gctaaagcaa tccaagatat
     3781 ggcacaaaga aaactgacat cggagttcaa acagctttca gagattatct ttgctgttgc
     3841 tggtttagtt ggaggattag gagataaata cgcaagaaat gcggcagcac atgctatgca
     3901 tgacgctatc agtaaatata ttccggaaag ccatcgtttt ctacatggag aaaaagtagc
     3961 gtatgggatt ttctatcaac tggcactaga aggtcgctgg gaaaaaatag acgagttata
     4021 tcctttgtat cgggaattag aactgccggt ttctctagaa caaatgggat tatttccgaa
     4081 agataaacag ataatagata aaatggctga atttattgat tcgaaagaga aagtgcattt
     4141 gattcctata gaggtaacac agaaatcact gatacagtca atgatggaac tggaaggtta
     4201 tatcagaaag atctgaagga ggaggacaca tggaaacatt aagtgtagta caaagtttgt
     4261 tgattgcttt atgggtagcc gcggttatgt cccgctggtt aggtggagga gccacgttga
     4321 ccttacgttt ttctcctttg atgactggat tagtcgtcgg gttgatcatg ggggaagttg
     4381 cacaagcgat gattgttaca gccgctttgc aaatgattta tatgggggtt ttttctccag
     4441 gaggatcaat gccagctgaa ccatctattg cagcagctat cgcagtacca gtcgcattat
     4501 taggggatct gtcgccagaa gcagcgattg ccgttgcggt gccagtcgga ttgctcggaa
     4561 gttacttgta tcagtttcgt tttttcatca atactttttt aggtaaatat acagacaaag
     4621 ctgtagaaga tttgaataca aaagctatgg cccgatcaat tattctttat ccaacggttg
     4681 cctcattttt attattcgtt ccgcttgttt tcattgcttt atatttaggg gcgccattga
     4741 tcgctgatgt aatcaaagct ttagagggaa cagtcgttat ccacattctt gaagtagtag
     4801 gtggagggtt agcggcaatc ggtatcgcga caactgtata cgttatcggt cgaaaagatt
     4861 atctggtctt tttcttcctt gcttatttta tgagtgttgt tttgaaatct ttagatatta
     4921 cgatggttac ttatgccatt tttgggatta ttatcgcttt gatctttgtc cagtcgcaaa
     4981 aagggaaaca agtacagcct gccacaagca ataacaattc agcatctttt gatgacgaag
     5041 atgactatga tgacggattt taatttagga ggaatgggaa atggctgaaa taatggataa
     5101 tcaaacaggt gctcctgaag aaatcacgaa aaaagatgta acaaaagcct atctgagatg
     5161 gcattttgca aacgaaattc ctcactcttt tgaacgttac ttggcgccat cactactata
     5221 cggaatgatg ccaatcttga aaaaacttta taaagatgaa gactctttaa aagcagcata
     5281 taaacggcag ttactgttct tcaacactca attaagctgg ggtggcggtg tgattactgg
     5341 cctaatgtct tctatggaac agcaaagagc agaagaagag tatgaagaaa aagagatctt
     5401 gatgcaggat gacttgatgt ataacacgaa agcgggattg atgggggcac tagcaggaat
     5461 cggagatgca atcgattcag gaaccgtcca atatattttt atcgcaatcg cggtaccttg
     5521 ggcacaacag ggaagtcctt taggcgcact ttttcctttt atctgctttg ggttgtatca
     5581 ggttttacta ggggtatttt tcgcaagaca agctttcact atgggccgta atgctacagg
     5641 cctaatgcaa aacactggtg ttcagacagc gattgaaatg ctatcagttt tagggttgtt
     5701 tatgatgggg gtgcttgcgg gaaactatgt aaaagtagaa tcttcattga aatttgcgat
     5761 atccggacgt gagtttgtta tccaggaaat tcttgatcaa atcgtgccag ggatattacc
     5821 tttagctgtc gtaatgggtg tgtacttgtt ctatacgaaa aaaggattga aagtcacaca
     5881 agcattatta tggctgactg gaatattaat tgtgctagca ggaatcggta ttttataaaa
     5941 agaagggaac gtgaataaga tgggtgtagt caatttagcg cgtgtggatg agcgtttgat
     6001 ccatgggcaa gtaatggtaa cgttatctca aaaaagtgga gtgaattcca tttttgttgt
     6061 agacgaagtt gtagcaaaag ataaatttat gagagatctt tacaaaaatg caggaagccg
     6121 tacaggacaa aaaacaattg tcattacacc agagaaagct aaattctatt gggatgaata
     6181 tcaatttaaa gaatacaatt gtattttgat cgcaaaaaca gtccaaacga tttacgactt
     6241 agtaaaacac ggaatcccca tggaagaatt gaatattgga gggattgctc aaaaaaatcc
     6301 tgatacagat cttttagtga cgaaatctgt ttatttaaat aaatcagatg ctgaaaaatt
     6361 gaaagaaatg aatgaacaat atggcgtaaa gaatatttat tttcaagcca ctccatctgc
     6421 acctaaaaca ggattaaaag atgtattgaa caagtttgat ttgtcataaa caaatgctta
     6481 gaaaaaagat aaagagggcg aaaaaatgaa gaagaacgag ctgtttcgtg attgggaatt
     6541 tcgttatcgc tatatttacc gtaagcgccg tacaaaaaag agtaaacagc gttttctttc
     6601 agcattagta tcagatattt acagtatgag aacagatgtg acagtcattg cttacgatac
     6661 gctagcctac cgatcaaaaa atatttatgt tggagatatc gaaaaagcag agaaagttat
     6721 ctgtacttat tacgatacac ccgttcatgc attaggaagt tatttcatgt ttgattggaa
     6781 agaccaaagg aaaaaaacaa tctactctat attattgtcc ttcattttgt tgttcagttt
     6841 aggttggtgg ggaatgatga tatataacaa aaatcctcat cacgtattcg atctgctatc
     6901 cgtataaaca ggtatcactg tactagcatt tgggagctac tttttcttat tgggaaaagc
     6961 agctcgcgga tggcctagca gacaaacttt tattcgaaat acttcatcca tattaacgat
     7021 gctagaaatg atccgtacga ttgatgatcc gagtgtagcg tatgcgtttg ttgatgaagg
     7081 ctgttatgga gaaaagggct tagacagcgt gagatcgggt atgaaaaaag aggctattct
     7141 tttttatcta gactctgttg gggcagatac accgctacag ttttcgggaa attatttttc
     7201 aaataaagaa cagtggctga aacaagttga taagttgaaa gaaaaaaacg tcaattatat
     7261 ttttagtgca agaaaaaagc aagcgcagtt cttctatttg accaaaacag atttacgagg
     7321 gaaaacattt aattggcaaa atgccaatca gattattgct ctttttcgat aagtaaaaag
     7381 tgcaaaacaa ggagtgagaa gactaatgtt ggaaatcatg atcgttacac atggtaagtt
     7441 gagcgaagga ctgaaagatg cggcggaagt aattgtcggg acatcgagcc atattgaaac
     7501 aatgtgctta gagtctgggg aggacatcca aacattcgga gagaaggtca aagaaaaatt
     7561 aactatgatc aacacagcag aacccatgct agtatttgtt gatctaatta gcgcaagtcc
     7621 gtacaatcaa gcgctcttgg cagtcaatga actgactgaa gaaaagaaag cagccactta
     7681 tgtcataggg ggcgtgaatc tgccaatgtt gttagaagca atcaatcatc agatgcttca
     7741 cacaccgata gaagaagcgg tggaacaaat tgtcaaacaa ggaaaagata atttaactgt
     7801 ttggcatgct tcagcggaag aaaaaaaatt agatgaggac gatttttaga aggaaggctg
     7861 aaaagatgaa gtggggattt agatggtatg gtgaaaaagg ggattcgatc ccattaaaat
     7921 atgttcgcca aataccggga ataaatggga taattggaac tttgctgact aaaatgcctg
     7981 gagatgtttg ggagatttca gaaattgagg agttgaaagc atctgttgag agccaaggac
     8041 tggaactgct tggaattgaa agtgtagcga tccacgatgc aatcaaggca gggacagatg
     8101 aacgagatta ctatattgat aactatatac gaacaatcaa aaacttgtct cgatgtgggg
     8161 ttcgattgat ttgttatagc ttcaaaccaa tttttggatg ggcgaaaact gatttgttta
     8221 atgaaaacaa cgatggaagc tattctcttt tatacgatca gaaaatcgtc gatcagatgg
     8281 agccaggaga aatgtatcaa ttgatacaca gccagtcaaa gggattcaaa ttgccgggtt
     8341 gggaagaaga acggttgaaa aaattcgctc agctggaaaa actgtatgaa ggaatgaccg
     8401 aagaagaatt atttgaaaat ctgacttatt ttttagaaaa agtgattcca gtgtgcgaaa
     8461 aagcagatgt aaaaatggct atccatccag atgatccgcc atgggaactt tttggttttc
     8521 ctagagtgac gaaaaatctt ggcgacttga agaaaattct aaatgcagta gattctccat
     8581 ataatggact aacgctgtgc acaggatctt taggggcaaa tccggaaaac gatttagttg
     8641 aaatcatcca tgaggtaggt tcgcgcatcc attttgtcca cttcagaaac gtggagtttt
     8701 taggagaaag aaagttcaaa gaaacggcac atccctctac agaaggctcc cttgatatgt
     8761 atcatattat gaaagcgttg gtgcaagaag ggttcgacgg tcctatacgc cctgatcatg
     8821 gtagaaccgt gtggggtgaa gtggctatgc ctggatatgg cctgtatgat cgtgcaatgg
     8881 gactcactta catgcaaggt ctatatgagg cgataagtaa ggaaaaacca ctgggagagc
     8941 attatgaata aaatcaagct cttcatcttt gatatggatg gccttttatt agaaactgga
     9001 cgtttagctt atcgtgctta tgtaaaatca gcacaaaagt atgattatga aatgcgtaag
     9061 gaagtctatt atttactaac tggccaaaca gaaatggcga taagagaaca aatgggcatt
     9121 ttgtacggag aagatgttcc atatatcaaa tggcgagaag caatcaatca atataaagaa
     9181 aaaatcgtga aagaagataa gagagtatat acgaaaaaag gagcagaaga aattttatct
     9241 tttgcaaaag aaagaggaat ccatacaatc gtggcttcgt ctaatacacg ggtaaaagta
     9301 gaaatgtatt taaaaatgga aaatctatat gccttgtttg atcaaatcat ttcaggggat
     9361 gatgtaaaaa aaggaaagcc ggaaccagag atctttttga aagcttgttc aaaaatgaat
     9421 attcctccaa gtgaagcact tgtatttgaa gactcgattg cagggatcga agcagcaagg
     9481 cgagcgggca ttttgtcttt tctcatcaaa gatcatttga tagggttgcc cgatcataat
     9541 gggaagcata agttgaaagt agatgtaagt ttatatgaat ccaataaagc agactatgaa
     9601 ttttatgacc tgcatcaagc aaaacgattt ttagaaagta aagggttgta tctctaataa
     9661 agaggactat tttctagtcc tctttattta ttgtcctgca cgactgaggg aacagatgaa
     9721 aaagaagtaa ttttttattt tgaagtatct accttatttg tagtgaacaa tttagaacaa
     9781 acacaaaacg catctactgt tatctaccag tagatgcgtt taaagagaag aacgagaatc
     9841 gtccatatga gggtttccac tccttatgca gattttttta attcttgcac tgcttgttcg
     9901 atcgtaacac ctgtcgctac atgattggta ttttctttgt caaaaacgac aaatagattc
     9961 aaatctgtat ccaaggttac tcgatattgt atatctttgt tatagaatgt catagccatt
    10021 ctcctttctt taaatacttg ctgaaaggca acgaacgcgt tgataacgaa attccattta
    10081 taagtatacc accaattgaa aggttttcat agttaagaaa ttttaaagtc aaaagttgca
    10141 gataaaagca aataaacaag aaaacaaaaa gtgaatcaat cgcggttatt caactttttc
    10201 ttcgattcga agacaataga agacaatgaa ttttatattt cattgcatta tccttgtgtt
    10261 attagaaaaa gcatgatata ttaaatgagt aatctatttt gaaacgtaat ttgagcgata
    10321 tatctacata gataaaactc cttttgccta tcaaatattt attgcaacga aaaaacgtac
    10381 attgatacgt atcaggagga atttatttat gaataacggt acagtaaaat ggttcaacgc
    10441 agacaaaggt tttggcttta tcactggtga agatggaaat gatgtatttg cacatttttc
    10501 agctatccaa ggtgacggct tcaaaacttt agaagaaggc caagcagtgt cttacgatgt
    10561 tgaagatggt caacgtggcc ctcaagcaac aaacattgtt aaagcttaat aaaaattgac
    10621 ttttaaccgt cttttcgaaa gaaaagacgg tttttttgta cgaaaaaatt
//
LOCUS       DAYYHX010000093        10651 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000093 DAYYHX010000000
VERSION     DAYYHX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.094"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..1040)
                     /locus_tag="ACHA6Y_001721"
     CDS             complement(24..1040)
                     /locus_tag="ACHA6Y_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8987815.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            1260..2348
                     /gene="chvE"
                     /locus_tag="ACHA6Y_001722"
     CDS             1260..2348
                     /gene="chvE"
                     /locus_tag="ACHA6Y_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8987816.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            2402..3928
                     /gene="mmsA"
                     /locus_tag="ACHA6Y_001723"
     CDS             2402..3928
                     /gene="mmsA"
                     /locus_tag="ACHA6Y_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8987817.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            3930..5135
                     /gene="mmsB"
                     /locus_tag="ACHA6Y_001724"
     CDS             3930..5135
                     /gene="mmsB"
                     /locus_tag="ACHA6Y_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM8987818.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            5392..6369
                     /gene="guaC"
                     /locus_tag="ACHA6Y_001725"
     CDS             5392..6369
                     /gene="guaC"
                     /locus_tag="ACHA6Y_001725"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM8987819.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            6633..8057
                     /locus_tag="ACHA6Y_001726"
     CDS             6633..8057
                     /locus_tag="ACHA6Y_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8987820.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(8099..8356)
                     /locus_tag="ACHA6Y_001727"
     CDS             complement(8099..8356)
                     /locus_tag="ACHA6Y_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987821.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            8518..9627
                     /gene="pdhA"
                     /locus_tag="ACHA6Y_001728"
     CDS             8518..9627
                     /gene="pdhA"
                     /locus_tag="ACHA6Y_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM8987822.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            9630..10607
                     /locus_tag="ACHA6Y_001729"
     CDS             9630..10607
                     /locus_tag="ACHA6Y_001729"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM8987823.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         3475 a         1815 c         2361 g         3000 t
ORIGIN      
        1 tttttgtcat gatacttctc cagtcaataa tttctggttg ggatgttagc tatattctgc
       61 tgggaaaaga cagtctcgga aataaaaata tctttaggta atgtttttcc tgtttgaata
      121 tttccaaaat aacgagtaac ggtattggct acataccagc cgacatcagg tttacactcg
      181 actacacaat tgacgattcc tgagcgtagc ttctcgatca tatcttcttg tccatcaata
      241 gttacaatga tcaaatcttc tccaggtttg attgatgttt cttttatcgc agctaaagca
      301 ccgtgagtca tttcatcact atggctgtat aatacatcga tttcggataa atcatttgtt
      361 tctaaatatc gtttcatctg gtcttttcct ttcatccgaa taaaatcacc gacaattcgg
      421 tgaacaatag tcatcggagg tttatggtcg atggttccat ctttttttag ggttggttta
      481 cgattgattg attccaaaaa tccttcggag cgtagcttgg tcggtgtgga gttctctgaa
      541 ccagcaagtt ctaggattct gatttcctgt ttctttgaat ttgtgaagtg attgctgaca
      601 aataaacccg cccgatttcc ttctgctttg aaactggggc caatgtgggt gacgaaaagc
      661 tcgggatcat tcgtttgaat atgtctgtcg acaagtatga cagggatccc tgctcgcttt
      721 gcttctttta atacagagtc ccacccggat tcttgcaaag gagtaaaaat gatcatatcc
      781 actttatagg caatgaacgt ccgtatatct tggatttgcc gttcctgatt catgtagcca
      841 tttcggtaga gaacttggta atcttctttt tcaagcgcag tgataattga ttcagtatgc
      901 tttttccgcc agctactctc cgttcctgat tgcgaaaaac caacgactag tgggtcaggt
      961 gcttcctttg tattaggtgt acaccccatc atttggtaag cgattacaac aagggctaaa
     1021 aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta ctatcttatc
     1081 agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa
     1141 aaaatccaag acaaaaaaag tacacctaaa aaatttaaag taaatcccat aaaaaaatca
     1201 atgttaacgc tttctttatt agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa
     1261 tgaaaaacaa atttagaaag attgttggga ctatcgctat tttgaccgca agcattctat
     1321 taagtgcgtg tggcaattca ggatctgcag atgatagtac ggggtatgta ggaatcgcta
     1381 tgccgaccaa atcagcagaa agatggatcg ctgatgggaa caacatggtc tcggaattag
     1441 aaaaactggg ttataaaaca gatcttcaat atggagaaga taaagtagaa aatcaagtag
     1501 cacaaataga aaatatgatt acaaaagggg tagatacact tgtgattgct tctatcgatg
     1561 gttcggcttt aactgatgtg ttagctaaag caaaagaagc agatatcaag gtgatcgcct
     1621 atgatcgttt actgatgaat tcagaaaatg tcgattatta cgcgactttt gataattttg
     1681 gagtaggtgt atcgcaagca gcttatattg aagagcactt aggattgaaa gaaggaaaag
     1741 gaccattcac gattgaatta tttggtggct cgcctgacga caacaacgca ctaatcaatt
     1801 acaacggagt gatgtctgtt ttacaaccat atatggacaa cggtcagttg gtcgtgccat
     1861 ctggacagac gagtttcagt caaatcgcta cgcttcgctg ggatggttct acggcgcaag
     1921 caaggatgga taatctgtta agcgcaaatt atactgacca aacattggat gctgttttgt
     1981 cgccatatga tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga
     2041 gtgaaagtaa acctttacct gtgattactg gacaagatgc gacagttgct ggcgttaaat
     2101 cgatcattgc cggggaacaa acacaaacga tctttaaaga cacacgtatc ttagcaaaaa
     2161 atacgattga aatgatcaaa gcaatcagtg atggagaaga ggttccggtc aatgatactg
     2221 aaacttatga taatggggtg aaaactgtcc ctacgtattt agcaaataca gtatctgtcg
     2281 ataaagataa ttatcaagca gagctgatcg acaccgatta ttataaagaa tccgatctga
     2341 aaaattaaga ggaaccaaac aaggaactaa agagatctaa gtcagagaga ggataagaat
     2401 tatggcggat tgtattttaa aaatggcaga tatcaccaaa aaattttctg gaatccgagc
     2461 gttagattgc gtgaatctga tgattgaacg tggggaaatc catgcattgt gcggagaaaa
     2521 cggagcggga aaatcaacat tgatgaatgt cttgagcggc ttgtatccat atggctccta
     2581 tgacggggaa atcttttatg atggaaagct gtgtcaattt aaagatttga aagatagtga
     2641 agaaaaaggc atcgtcatca ttcatcaaga attagcacta agtccttatc tttcaataaa
     2701 agagaatatt tttctcggaa atgaacaagc agaaagaggc gtgattgatt gggaactgac
     2761 agaaaagaaa acatatgaac tgctgaataa agtcggtttg tctatcgacc cgaatcttct
     2821 agtatcccag attggcgtag ggcaacagca actagttgaa atagctaaag cattatcaaa
     2881 atccgtccgt ctgttgattt tggatgagcc taccgctgca ttgaatgaag aagaaagcgc
     2941 gaatttgctg agattgatcc gagagttgaa agagcaaggt gtaacctcca tcattatttc
     3001 acataagctg aatgaaatcg tggatatcgc agatcgaatc accattttac gggatggaca
     3061 gacgattgag acactagaaa atgaaatgat cagcgaagag catattattc gcggaatggt
     3121 gggcagagat ttagttgatc gttatcctga aagaaaaccg gatattggag aagtttattt
     3181 taaagtagaa gactggacgg tctatcaccc tcttgatcac gagcgaatca taaacgacca
     3241 catcaacttg catttgaaaa aaggagagat tgtaggaata gctggactga tgggagcggg
     3301 cagaactgaa ttcgctatga gtctatttgg acattcttat ggaagcaaga tatccggacg
     3361 tatattaaag gaaggggcag agatatcagt taagaccgtt ccttcagcca ttgcaaacgg
     3421 actcgcttat gtctcggagg accgaaaatc tttaggactg aatctgctga tggatattcg
     3481 ggaaaacgtg acgctgagca gtttggataa aataagtcaa aaaggagtat tagataaaga
     3541 aaaagaagta actgaggcag aaaatttccg gaaaaagatg cgtataaaaa caaactccat
     3601 ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg
     3661 gctgatgaca caacccgata ttcttttttt ggatgagcca acccgtggga tagatgttgg
     3721 ggcaaaatat gagatctaca cgattatcga agaaatggca gcaaatggca aagctgtctg
     3781 tcttatctct tctgaacttc ctgaaatatt agggatgtgt gatcgaatct atacgatgaa
     3841 cgaaggacgt attacaggag aagtctcgcg agaagatgca gatcaggaag tcttgatgaa
     3901 attaatgacg aaagaagagg tgggttaagt tggaacaggc aaaaaatctt acggataaaa
     3961 aagagaaaaa actaaatttg aaaagtagag cagctgatct ttttggtcgc tacagcatgg
     4021 tgatcatttt agcgggacta cttattgcgt ttcaattgat gacggatggt attttctggc
     4081 gtccattgaa tataactaac cttgttctgc aaaatagcca tatattagta ctagctgcag
     4141 ggatgttatt agttgtgctc ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt
     4201 ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca aatgaacccg tggatgacag
     4261 ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt
     4321 atgcagggat accggcgttt atcgtgactt tagcaggatt gctgatgttt cgtggactga
     4381 cacaagttgt tctaggaggc caatctctag caccttttcc agctgttttc caaaaaatgt
     4441 ctacgggtta tttaccagac ttaagcgaag gaagcagcct tcacttcttg acattgattt
     4501 taggaatgat catcacgatc gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga
     4561 aaaatcattt atttgaagtg gaatctccaa cagctttctt ggtaaaggcg gtatttacag
     4621 cagtcgttgt actaggaata acttatatct ttgcctctta tcaagggtat ccgattattt
     4681 tgatcattct tggtttgatc gttgcagcat atgggttttt gacaaataaa acagtagcag
     4741 gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa
     4801 caaagcgaat cacgttctgg gtattcgtca acatgggagt catggcagct ttagctggat
     4861 tagttttagc tgctcgattg aatgcggcaa cacctcaagc aggtacatcg atggaactgg
     4921 atgcaatggc ggcagtatat tttggtggtg cttcgacttc cggcggcatt ggaacaatca
     4981 tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg tcgattttag
     5041 gcgtcggggt agactggcag caggcaatca aaggattgat cttgctttta gccgttgtgc
     5101 tagacttgta taataagaaa aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa
     5161 gactgtgaca agacatttgt cacagtcttt ttttcgctaa aagcgaacat tcattgtttc
     5221 gtttgtatag actaaaaata atatatgttt ttttaccatt cgtataaatt ctctttttgc
     5281 tcttgatttt tttgcttaat tgagttaaat tacatagggc gctaaaaaaa gccttgatta
     5341 aattaataat gttcgtgttt tgctggatgt gaacatcagg aggataatgt aatgaaagta
     5401 tttgattacg aagatattca attaattcct aataaatgta tcgtaaatag ccgttctgaa
     5461 tgtgatacaa cggtcacatt aggtaaacat acattcaaaa tgccagtagt gcctgccaac
     5521 atgcagacga ttattgatga aacaatcgca gaattcttag cggaaaatgg ttatttctat
     5581 atcatgcatc ggtttgatga agcagctcga attccgttta tcaaaaaaat gaaaaagcgt
     5641 ggattgatta cctcgatcag tgttggcgtc aagaaagagg aatattcctt tatagagaag
     5701 ttggcagaag aatcgctaaa tcctgattat atcactatcg acatcgcgca tggacatgct
     5761 aactcagtta ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac ttttgtgatt
     5821 gccggtaatg ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg cgcagatgca
     5881 acaaaagtcg ggattggtcc aggaaaagta tgtattacaa aaatcaagac aggctttggg
     5941 actggaggct ggcaactggc agctcttaga tggtgtgcga aagcagctcg caagccgatc
     6001 atcgctgacg gtgggatcag aacacatggc gatattgcaa aatctgtccg ttttggtgcg
     6061 acaatggtca tgattggttc attgtttgcc ggtcacgagg aatctcctgg cgaaacaaaa
     6121 gtcgaaaatg gaatcgtgta taaagaatac tttggttctg cttcagaatt ccaaaaaggc
     6181 gaaaaacgaa atgttgaagg aaagaaaatc tggatccaac acaaaggatc tttgaaagat
     6241 acactagtgg aaatgcaaca agaccttcaa tcatctattt cttatgctgg aggaagagat
     6301 ctggaagcca ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt taatggagac
     6361 acaatctaaa aaataaataa aatattagga tgttgtgaaa aagttgtcct gcattaagta
     6421 ataagaacag ccaaacaaaa atagttcttt atatgttttg tttggctgga attattatcg
     6481 cagatacaag cttttgaaca tcattattca gaataaggga ctgtgataag atttttgtca
     6541 cagtccctta tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg gtaaaatcaa
     6601 aagaaaaaat agacaataga aaagagaagc ttatgtggga aaaagtagag aaaaaacaag
     6661 gaccgattta tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac ggaaggatca
     6721 atcctggaag tcaacttgaa tcagaacgaa aactagggca actttttggt gtgaatcgtt
     6781 ccaccgttgt ccatgcattg gaagagctta gggagcttgg ggtactaacg agcaaacgtg
     6841 gcagcggatg gtttgttaat cagactgaat gggggaaatt cgctgttcca agaatcgatt
     6901 ggcggcaaat gatctctcct cgttatgagc agacggactg gtatgagcaa aaaataagag
     6961 aagcaaagca gatccaaaaa acatcgtttc ttgatttata cgctagcgag atgccagatg
     7021 agcttttgcc gaattttgag tttccttctt attctcttga agaaatctta gcggaagaaa
     7081 aagaaatgga tcttttaggc tatactccgt tacgtaacaa aatcaaacgg tatttggaaa
     7141 gtaaatttga attggcattt tcccccaacc agcttcttgt gactggtggc ggtcagcaag
     7201 caatcttttt gattttacaa acgattcttt cagctggaga ggggatagct gtagagtcac
     7261 cttctttttt ttaccgatta cctcttttca aagcttcagg aacgcgttta tttgggatac
     7321 cgatggatga agaagggatc gacttagaaa aactgaaaga atcgatccat aaaaataaat
     7381 tgaaagcagt gcttatcaac ccgaattttc aaaaccctac aggaaaagtg atgagtcaaa
     7441 aacgacgaga aaagctagta gaattgtgtc gacagtatcg gcttccaatt attgaagacg
     7501 atgtatttac ggatctttca tttctaaacc aagaagaagt ccttcctata cgaacgattg
     7561 atccggataa tgtgttatac gtcggttcgt tatctagggt tttaggaaaa acaacgaaaa
     7621 tcggttgggt gataggacct gagactttta tctcgcaact agctcaagcg caagaaatga
     7681 tggagttttc gatgaatatc cttacacaaa tcacagtggc taatgtattt aatgaagcaa
     7741 ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga ctgataagcg
     7801 cttggggaga ttcacaatcg tttttcaagc ttcattcaat tttaggtggt tactatgcct
     7861 ggattacttg ggatgggaaa aaactagacc ccgaactagc agaaaaagta gtagatatgg
     7921 gtcttggaat tgcaccaagt ctactttttg ggcaagatat aaatggaatc agaatcaatt
     7981 acagtaggct ggatgaaaac caaataccgt tgttttattc aaagatgaaa attctagaaa
     8041 agtggttaac agaataattt cacaaagtaa atagattaaa gaatataaca gcttggattt
     8101 aaatttcaag ctgtttcttt tttttcggaa aaagaatacg atataaagaa actaagaaaa
     8161 aaccattatt tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg caacaaaact
     8221 gtataacacc aacgttttta aatagaaaca gatggttgta agtttgatta gaaaatagaa
     8281 aaaatgcaga tgaaatataa aacgctttcc ttattgtgat tttattcact aatgctgtta
     8341 atatgcggat tggcataatt tcctttcatc gtgaaaattt gtgcttttag cttgtatttc
     8401 tcataaatcc atgataattt attaatgaag acggttaaat agtgatacag ttttattcac
     8461 tgtttcaatc gcagaatgaa gagattttct tatagatgag agaggaatgt gtgacaaatg
     8521 gcaaacaaaa aagcaccgat cgacttccaa gcactgctag atgaagtcaa tgcagatttc
     8581 ccagtatatc aagcacttga tcaagatgga aacgtagtta acaaagattt agtacctgat
     8641 ttatccgatg atgaactcgt tgaattaatg actcatatgg tatggtctcg tgtattagac
     8701 caacgttcaa cagcgctgaa ccgccaagga cgcctaggct tttttgcacc aactgctgga
     8761 caagaggcaa gtcagctggc tagtgcttac gcatttgata aagaagatgt tcttcttcca
     8821 ggttatcggg atgtacctca actagtaaaa catggtttac ctttatctca agctttttta
     8881 tggtctcgag gacatgctgc aggtaattct tatccggaag aattgaaagc tctaccacca
     8941 caaattatca tcggtgcaca atatgtacaa gcagccggtg ttgcattagg tttgaagaaa
     9001 cgaaacaaga aaaatgtcgt cttcacttat actggtgatg gcggttcatc acaaggtgat
     9061 ttttacgaag caatcaattt cgctggcgct taccatgcaa atgccgtatt ttatatccaa
     9121 aacaacgggt atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa aacacttgca
     9181 caaaaagctg tcgcagctgg gattcctggt attcaagtcg atggaatgga tccgcttgca
     9241 gtatatactg tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg accagtccta
     9301 atcgaaacac tgacttatcg ttatggacca catacactat ctggtgacga tccaacccgc
     9361 taccgtacaa aagacacaga tgacgattgg cagaaaaaag atccattgat ccgcttccgt
     9421 aaatatttga cggaaaaagg actttggtct gaagaaaaag aagaacaagt catcgaagca
     9481 actaaagaag aaatcaaagc agctattgct gaagccgaca aagtaccaaa acaaaaggtt
     9541 tcagatttct tgaaaaacat gttcgaagta tctccacaat caatcaaaga acaaattgca
     9601 atttatgaag cgaaggagtc gaaataatca tggcacaaaa aacaatgatc caagcaatca
     9661 cagatgcctt agcacttgaa ctagaaaatg acgagaatgt cgttgttttt ggtgaagatg
     9721 ttgggaaaaa cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag ttcggagaag
     9781 accgagtatt cgatactcct ttagctgaat ctggaatcgc aggattgagt ttcggtcttg
     9841 cgttagaagg atttcgtcca gttcctgaaa tccaattctt tggttttatt ttcgaagcaa
     9901 tggacgaagt agtagctcaa atggctcgta caagatatcg aatgagcggc actagaaatc
     9961 tacccatcac aattcgttcg ccatttggtg gcggtgttca tacaccagaa ttgcattcgg
    10021 ataatttaga aggtttgatc gctcagtctc ctggtattcg agtagtgatt ccttccaacc
    10081 cttatgatgc aaaaggacta ttgatttctt ctattagaag taacgatccg gttgttttcc
    10141 tagaacacat gaaactatat cgctcattcc gagaagaagt gccggatgaa gcttacgaag
    10201 ttcctttgga taaagcagca gttactcgtg agggaacaga tgtatctatt atcacatacg
    10261 gagcaatggt gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa aatatatctg
    10321 ttgaaatcat tgatcttcgt acagttgcgc cattagatgt agaaacgatc attcaatccg
    10381 tggaaaaaac tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg ggcgttgccg
    10441 cgcaagtagt ttctgagatc tcagaacgcg ctatcctttc attagaagcc ccaatcggac
    10501 gagtttctgc acctgataca gtcttcccat ttggacaagc agaaaatgtc tggttgccaa
    10561 atgcaaaaga tatcgaagac aaagtaaaag aaatcgcgga attttaagaa aagaagggac
    10621 gaaacaaagt catggcttat caatttaaat t
//
LOCUS       DAYYHX010000094        10442 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000094 DAYYHX010000000
VERSION     DAYYHX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.095"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..329
                     /locus_tag="ACHA6Y_001730"
                     /pseudo
     CDS             1..329
                     /locus_tag="ACHA6Y_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(327..1046)
                     /locus_tag="ACHA6Y_001731"
                     /pseudo
     CDS             complement(327..1046)
                     /locus_tag="ACHA6Y_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(1071..1256)
                     /locus_tag="ACHA6Y_001732"
     CDS             complement(1071..1256)
                     /locus_tag="ACHA6Y_001732"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM8987824.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            1483..2756
                     /locus_tag="ACHA6Y_001733"
                     /pseudo
     CDS             1483..2756
                     /locus_tag="ACHA6Y_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2863..3894)
                     /locus_tag="ACHA6Y_001734"
     CDS             complement(2863..3894)
                     /locus_tag="ACHA6Y_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8987825.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3901..4731)
                     /locus_tag="ACHA6Y_001735"
     CDS             complement(3901..4731)
                     /locus_tag="ACHA6Y_001735"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8987826.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4728..5543)
                     /locus_tag="ACHA6Y_001736"
     CDS             complement(4728..5543)
                     /locus_tag="ACHA6Y_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8987827.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5540..6364)
                     /locus_tag="ACHA6Y_001737"
     CDS             complement(5540..6364)
                     /locus_tag="ACHA6Y_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFM8987828.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6354..7454)
                     /locus_tag="ACHA6Y_001738"
     CDS             complement(6354..7454)
                     /locus_tag="ACHA6Y_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8987829.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7632..8417
                     /locus_tag="ACHA6Y_001739"
     CDS             7632..8417
                     /locus_tag="ACHA6Y_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8987830.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8450..8833
                     /locus_tag="ACHA6Y_001740"
     CDS             8450..8833
                     /locus_tag="ACHA6Y_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8987831.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            8909..9040
                     /locus_tag="ACHA6Y_001741"
     CDS             8909..9040
                     /locus_tag="ACHA6Y_001741"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8987832.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9009..9245
                     /locus_tag="ACHA6Y_001742"
     CDS             9009..9245
                     /locus_tag="ACHA6Y_001742"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8987833.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            9257..9343
                     /locus_tag="ACHA6Y_001743"
                     /pseudo
     CDS             9257..9343
                     /locus_tag="ACHA6Y_001743"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(9323..10294)
                     /locus_tag="ACHA6Y_001744"
     CDS             complement(9323..10294)
                     /locus_tag="ACHA6Y_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFM8987834.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3090 a         2450 c         2021 g         2881 t
ORIGIN      
        1 actcagatgt tgaaagtcaa agaaaagttg actattttag aattaaaacg tacccgtttt
       61 ctatgctatg attgtgggca gaccttttct gcgaaaacgg acttagttga tgaacatcat
      121 caactaacaa aggaactgaa acaggcgatt ctcatagaac tgtacgaaaa tcaatcacgt
      181 aatctgaaaa tcagaagaag taccgtcggc tcaagcgata ttggaaactc ttactcaaag
      241 attccacgac attggagccg ctcaaacggc attatcatcg actgtttaaa cgaccaatta
      301 gtcaaacaga aatcgtcgat gaaccgttgt gcacaatggt gtcctgaacg tacagcgaca
      361 ccttccatat ccagagcagt cgccacatca cggggatgga tgccatccaa attaaaagca
      421 accacgccag tatgatgttc aggatcttgc ggtccgtaaa tggtgatacc gccgatcgct
      481 tgtaatttag gtaaaacgta ggcaaccagt tcttgctcat aacgatgaat attctccata
      541 ccaatctcat tcaagtaatc gattgctcct gcaatgttcg gagtaccagc ttcaaatttc
      601 catggcagct ctttccatga actatcgtaa agattgacaa aatctatcat ttcaccgccg
      661 aattcgactg gctccatttg ttctaacaat tgacgtttgc catataatac accgattcct
      721 gtcggcccgc acattttatg cccgctaaag gcgaaaaaat ctgcatccaa cgcttgtaca
      781 tcaactgtca tatgcggtgc tgcttgggca ccgactggac tatcctcaac tacaacagcc
      841 tgttcaaccc tcagccgttt caagcacaac aaataacctt ctggatctgg tttgccgacc
      901 tgaagatcat cgatcgtcac aaaggaatca attttttcct taatcatcgg atgtgcttca
      961 aaaaaatagt ctagctcccg cagttcagaa ttgagactac tccgattttc cagtgctgct
     1021 gatccgctgc tcaaaatgtt cagcatccac tggtctccca accgcttgac ttatcccttc
     1081 ataaaaggac agttctgctg ccgtcccaac accttgttga tagtctgcaa aaggaatttt
     1141 atagccgaac tgctccagta aataggcttc agtaaatagt gatgaatact ctcgtaaaac
     1201 atccattcac tgtcaatcat cgtcccatca aaatcaaaaa taagtgcttt ggtcatcttc
     1261 tttccccctt acccaaaaca agcaaaagct gtgtcctgct ctctctacag tatcagcata
     1321 aaaactgccg tctgaaaagt caaaggtgaa agaaatgtgg cgctataatt tctaggactg
     1381 atagtaaatc attctatcag tccttttgag cataaaaaaa caaaacatac agaagctccg
     1441 ttagaattga atcaccacaa cacaatcaaa ggagcgttct gtatgcctga tcttcatctt
     1501 atcaaactat tactaggaat caaagatgga aacattcaaa tcactcatgt cgagaagaaa
     1561 aaaatacatt caatcaacag tatcgttgtt tctgccacgc tcattaaaac gattcagccc
     1621 tgtcctcagt gtaaacaggt gaatcaacat gggctgatcg ttaagaacgg aaagaaaaaa
     1681 tcgctggtcc ttctcaataa gtgtgcgaat caacggactt atcttgcttt ggcaaagcaa
     1741 cggtacctct gtcgtggctg tcacacctat tttacggcga atacctacat tgttgaaaaa
     1801 aactgtttta tcgccaaaca agtacattat aaaatattgg aggaattgac tgaaaaacaa
     1861 gcaatgacga cgatttctaa acattgcggc gtttcttggt caaccgtttc gcgaacactc
     1921 gcttctctgc tccccatgac aaacgtgaaa ataaattggt tgcctcgctg ttcattggta
     1981 gatgaatttc gttcattgaa aaatcaagtc ggtccctatt ctttttcttg tatggatggc
     2041 gatactggga aattactgga tattcttcct tcacgtaaga agaaagatct catttcttat
     2101 tttatgcaat ttgaacgacg tgcacgttta aacgtaaaaa tactcgtaac agatatgaac
     2161 gcctcttatg ctttgctgat aaaagagtgc tttcctcatg ccaaactcgt tgtggatcgc
     2221 tttcacatcg tcaagcacct cattcgcagt tttaaggata tttgactatg agtcatgaaa
     2281 agcttcgacc gaaacgatcc aatacaagcc aaacactatc gacaagtgaa ggctttatct
     2341 cgacttctga tcaaacgaca ggacacgctt gtttatgata aatggacaaa atggcgtaat
     2401 tttggttggg cctatttaac cgaaagtgaa gtcgtagaac gtctactttc tacttctgat
     2461 gaactacgaa tagcttatgc ttactatcaa gaaattcttc aagcttttta tgacaaagaa
     2521 gcggacatct tctttcagct tgtaaaaaca atgcccaagt cggttccaag agaactgcat
     2581 catataaaaa aagcgtttat aaactatgaa tccggcattc gtttagcctt agagttgcct
     2641 tactcaaatg gcaaaataga aaatctgcat acccacatca aagcattgaa acgaatagct
     2701 tacgggttag gatgagaacc agaattttct taatcaatca tctgatcact tattaaacat
     2761 aaaaaaacat ctaaagcaga aaatcaattc taccttagat gttcttatat cactctatca
     2821 gtcctatttg acaaagagcc agaaatgttt gccgactata cactagcttt gttgtgcaaa
     2881 atgctgagta tcaatttcca cagggattgc tagaatatct ttcagctgaa tgtataaggc
     2941 atcgctaatt ttctcttccc gatcgttcaa ccgatccact aaaatttctt tgccctccag
     3001 cgtttgaaaa acataccgtt gaatgttgcc taaaataaat gattccttcc atagaaccgg
     3061 aatcttaacc ccctgaggaa ccggttcaaa ttgaatcagc tccggtctta aataataaat
     3121 gcagcgattg cccttagcgg aaaactcgtg ggtatactga ttcagctcat accattcgaa
     3181 acgattatag ttgccgataa attccgcaac aaatcggctc tgaggattct gatacaattc
     3241 cttcgaggtg gaaacctgag ccacccgccc agcttccatc acaaaaatcc gatcactgat
     3301 tcgcatcgcc tcttcttggt catgagtcac aaaaatcatc gtcatcccta gcgattgctg
     3361 gatagcgcgc aaatcccgct gcagctgttt tctaattcgg gcatccaaag cacttaatgg
     3421 ctcatccagc agcaggactt tcggttcagt gaccaatgag cgagccagtg ccacccgttg
     3481 tcgttgaccg ccagaaagaa actggacatt tttatctgcc tgctccgtca tatccaccaa
     3541 tgatagcatc tcaaaaacct tttcctgaat cacctcagga ctcagcttct ttaccttcaa
     3601 accaaaggct acattttcaa aaacagtcat agttggaaac aatgcatact gttgaaaaac
     3661 aaagccgata ttgcgatcct tcgtaggctt atcggcaata ttctcattgt ccagcacaat
     3721 ctcaccgctc tttggttgtt ccaatcccgt gatacagcgc agcaacgtag actttccgca
     3781 cccgctggga cctagtaaag taaccagctc acccttttca atctgaattt catcaatagc
     3841 taaaatctgt ttttcatcaa aaaacagatt gagatcatgt atttctaaaa acattttccg
     3901 tcacaactcc tttaagggct tctttttcga ctgcttctct tccctcgtct gcttaaggga
     3961 cagctgttgc ttttgtcgat aagtcagcat aaaggtagtt aaagcaattc ccaccataaa
     4021 cagcaaatac aaaaccatca cagcgctagc caaatggccg ttttcgttca tccgccgcag
     4081 catatagatg cgtaccgtct gaaatcgacc gccaatcaac aggttcgtca gcatgtactc
     4141 cccaaaaacc gaagaaaaaa tcatcagaga aactagggtc acaccgatac ggatgtttgg
     4201 aaacagcacc tggataataa tcgtagacat cggggcgccc aaggtttctg ctgcatccac
     4261 taattccttc agattaatta agcgcatctg gttgttaaga ctaagataga caatcggcaa
     4321 cgccgtaata aacaatgcac ctaccaacac tacaaacatt gggataccag tttcagcata
     4381 cgttcgaagc aaagccgtta ccaaaatcac tcctggtaaa gcatacggca gcaggagcag
     4441 cttttccagc caacgattca aacgagggaa atacaaatga atccaaataa tggtgggaat
     4501 catcaacaac agaatcgtca ccaagaccac acctgctaat aaaagtgagc gacccaccgc
     4561 agcaataaat tccctgtccg tcagcagctg ttggaaccat tgcaaggtaa aatcactagg
     4621 taaaagcgat tttgaccatt tagtggagct ggcatacaaa atcgttccaa taatcggcag
     4681 caagtaatac aggacaaagc agaacaaaat cggttttatt aactttttca cgtctgcacc
     4741 accttgcctt tcttttgaat aatcaacatt gccgttacca gcaggacacc taaagcgact
     4801 gccaacgcac tgcctaaatt aggcttggcg aacacatctc ccgacactaa agccgcaatt
     4861 cgaatcgtta aaagatttac attactgctg gtcagggcat acgctgtttc ataggtcccc
     4921 atgccattag caaataagat gacaaaggtg ctggcaatcg atggtcgcaa aaaaggcaaa
     4981 gccaccttga accaagcata cgctgatgaa gcgcccagca attgtgcgga ctccagccat
     5041 tcctttttca cttccttcat gctgggatac aaaaacaaga cccctaacgg aatttggaaa
     5101 taggtatacg tgatggtcaa tccccaccaa gaatacaaat tgaagtccgc tagaaatggc
     5161 aaaatcattt tcagcacacc tacattcccc aacaaaataa taaaagagaa tgccagcgga
     5221 atcccagcaa aattagctgc caaattggaa acaaaggtga ttttctcctg caatttagtc
     5281 gatagattca ttaagcataa acataaaata aacgtcccga ttaacccaat cacactggag
     5341 ctgacagcaa tcagtacgct gttaacaaag gcctgtgtgt aatatttgtt gctgaaaata
     5401 tcactgtaat tggccaatgt ccactggtta gttaaatcac tgtgcaagct gcacagaacc
     5461 ataaaaagca gcggcagcag taaaaatgca cagaagataa aggaggcagg aaccagaacc
     5521 aagcgttttt tcatctcttt catcccctca tcaattcttg aatgtcctgc attttctctg
     5581 acggtgcaat tcctaataac cagcaaaaca atggagccgc cagcagctgt ggtacagttt
     5641 gttccttcag cttttggcgt ttatttgaaa tcaaataaaa aggaacttca cgatgtgcag
     5701 ccaagctgcc gccatgcagt ccgtgcgcat ccatcccgtg atccgcagtc accacgactt
     5761 gataaccttt ttgcagccat tcaggaaggt aaatagccaa taatctgtcc gcctgattaa
     5821 ccgctgctgc atattcatga gaatcggcgg taaatttgtg accgacatca tcaatattca
     5881 tcgaatgtac gaccaaaaag tctggctgat actgattcac taaataatgg gcatccgcaa
     5941 ataaatgcga atcagggtaa gtgtcctcac tataaaagat gccatgctga atcagtcgct
     6001 cctcctgatg ctggatgcga tcaaccaaac gattataggg ggcttgattg tacaactcgc
     6061 tgtaccagtg gtaagcggcg gctcctgttt ttccgccagc ttccttaact aattcaaaca
     6121 gcgaccactc tttggatcgc tgagcatggt aattactata gatgccattt tcataggctg
     6181 gaacgccggt catcagtact tcgtataacg gacgtgaatt gctgggcagc tcacttttta
     6241 ccttcaataa gcttgcttgc tggtgctcaa ctaaatgatt catgaaccca agctgggcga
     6301 cggctgtgtc gtagcgacag ccgtctagta taactacaat cagtttgtca agtctacttt
     6361 gcataggtca gtacctccgc ctgccaaagc tctggaagct ctacagaggt cttgttccaa
     6421 gcttcattgt cttcaactgg caccgccgct tcataagcat cctccggcag taatttttca
     6481 gctacttctt taggcagctc taccttgcga ataggtcgcg catagccctt tgccaaattg
     6541 atttgccctt catcagataa aatatattcc cgtgctagct ttgccgcatt gggatgaggg
     6601 gcatttttat tgattaaggt tgtatacccg ctaatgatgg agccgtctgt tggcacagtt
     6661 acctcaaaac gctcggagtc aatttggtca cgataattta gtccattgaa gtcccaaaca
     6721 actgccacat cgatttcacc tttttcaaga tttgctacgc tggcatcaac ggtggacaaa
     6781 cggccttttt ctgccatttt tttgtagtaa ttcagacctg gctgaatatc cttttcgtca
     6841 cctccgttgg caatcgccgc agccaataca gcaaattgtg cttggttggc aatcgtcaca
     6901 tcccccacgc taacttgata gtcaccctct tgcagctcct tccagctagt cggtgcttgc
     6961 ttcacattct ctttatcggt gataaaggaa atggttcccg tatagcttaa caaccaatcc
     7021 ccatcatcat cctttgccca atccggaatt tcctcccaat agttggtctt ataaggtaag
     7081 gttaactctt tgctttcagc caacggaccg aagctgatac cgacatcacc aatatcggcg
     7141 gttccatttt ttccttctga ttcaaactta gccaattctt ctgcactgga catatccgta
     7201 tccacgtgac ttaagccgta ttctttttca aggtcgttcc aagtgcctac ccaatttgcc
     7261 caagtatccg gcattcccaa gctggcaatc tctccttctt ccttggcttt ttctataatc
     7321 gtatctaaag agtcgtctgc cgcagccgat tgcttcgttg cttcacctga gccgcagcct
     7381 gtcagcagga tacttactgt tgctgcacaa aataaaccga tagtcatgtt acgaattttt
     7441 tcattcattt tcattcattc ttctcccctt tcgataattc cattctaaca aagctttgta
     7501 atgagagaat cagtttcttg aaaaggtttc atgaacaatg taaaggttgt atatggtttg
     7561 taaatttcga cgaattcccg ttaaccttct aaagcttttg cggtatgata gtagaaaaaa
     7621 ggaggctccc tatgcagtat tatgatcagc atctccacac gtatttttcc tttgattcag
     7681 aggaaaaatt tgaaaactat ttagcctatc agccggagtt tttcgtttct acggatcact
     7741 ttgatttaaa caatccatct accggaaaag atgacattcc ggattatcca gcctatataa
     7801 acaaactgga ctctttaaaa gaaacctatt ccaccaaatt tctgcgaggt atcgagattg
     7861 gcgttgttcc aggacaagaa gagcggatcg tggattacct cgctcagcat ccttatgatt
     7921 taaagctggt aagtattcat caaaatggcc gctttgatta tatggacgat gtggttttaa
     7981 ccaaagatcc cattcaaact accaaggaat attttgatca gatgttgcag gtcttgacgg
     8041 attttccaga aggagatatt ttgactcact ttgattatgg attacgccgc ttcaccttca
     8101 cagcggaaga attcgcggag cattttgagg ctcagctgct aacgattttc aaaaaggttg
     8161 ttgaacgaga attggcgatg gagttaaatg ccaagagctt cttgaaatac ggcaacgaag
     8221 acctttaccg ttatgccgtt cctttgtatc aaagtgtcgg cggaaagctc tttaccttag
     8281 gctcggatgc tcatgttgcc gaagattatc agctgggctt taaaaaaatg agtcagctgt
     8341 tggcagactg tcaggtagaa aagttgctag tcattcaagg aaaagaacgc cgcctgacgc
     8401 cgctgccgat tttgtgattc ctatataata gaagaaagtg gtgaaaaaaa tgagcaacct
     8461 gtgggactac aatcaagagg cacccatcca ttatttaatt gcgcgacatt gggatgcgtt
     8521 gaaaattgag gcggtttgcc gatccttgtt agcagccgtt ccaaaacaac aactggaaaa
     8581 ttttttggtg gctgacagct tgcagcggga aaaagttcag gcttattttg cagcctttaa
     8641 agatcagcct ttggaatatt tgcatgcgca gtttcatctt ttttatcaag tagccgcccc
     8701 agatgactac aacgatttac gaggacagct gcaattgacc tttcaagcgg atgaaaccgc
     8761 ctataccgtt ttattgggaa tggctcgcct tggtgatcag gcgaaggtcg agtggcggat
     8821 tttcgatatt tgatggcagg atcaaggcgt ttcagttgca aaccttcctc tctttcaatt
     8881 agaaaaaccg cagcaggaag aagtttgtgt gctggtcacc gatgctggtg aaattcgtct
     8941 ccgcttattt cctgaccaag ctcccctggc tgtagccaac tggcgctccc tagctgagca
     9001 aggcgtttat gacaacacac ctttcggctc gtgtcattaa ggaatttgtt attcaaggtg
     9061 gcgctttgga tggttctggt gatgaagctg tttccagcta tgagggcttt ttcgctgatg
     9121 aagtgcatcg cggactttat cattttaatg gcgccttggc cttaggggac cacggccccc
     9181 acaccaacgg caaccagttt tttattgtgc aaaacacaaa ggcgcaggct gacctgctaa
     9241 tgtaaagcaa gcatacttgg agcttggagg actaccggag ctggatggtc gttatactgt
     9301 ttttggacag gtataccaag ggttagaaat cgtagcagcg atcgcagctc agccgacaaa
     9361 cagtgaagat gtaccgattg agccagtgtt cattcgacaa attaacttcg aaaaaacatc
     9421 gtaaaaagac actcactgac ttcagcaaaa gctctattct tgagctggct gcagctagtg
     9481 agtgtctttc atttatccat aagataaagc ttggattcaa taaataaata aatgttttgg
     9541 ttaaagctgt gaccagcaaa tgaggaaccg tgcgagccag ttgatctgcc aatgcaaaca
     9601 tttctttagg tgaaggcgct ggcgtgtctg ccaacggata ctccagtccc aatgcttggt
     9661 aaccgtgcat accatagcag tggtacggta gtaattctaa cggtacgaac cgatcaagat
     9721 ctgccacaaa acaagcaatc gtcagcagct cggtcaggtt catattgata cctggaacca
     9781 caggaactcg aattcgcact tcgcagttag gatactcaat taaaaagcga cgaatattct
     9841 tcaaaatttg ttggttatct aagccggtcc attgtttgtg gagcttgctg gtaaacattt
     9901 ttacatccac aaacaatgta tccacatagg gagctacctt ttggatttca ttataagcac
     9961 cacaaaagct ggtttccagt acggtattga ttccaatgta tttcgatttt tgcaaaattt
    10021 ctttagcaaa atccgcctga accagtgcct cgcctccgct aatggtcact ccgccatccg
    10081 agtgaaagta aaaaacagca tccttctcaa tctcatccat tacctcttca gcggtcatgg
    10141 tctggccaaa tcctttttca atctgcttgc tttgtgattc gggaatcgaa caccaggcac
    10201 agcgtaatgg gcagcccttt aaataaacaa cggtccgtaa accagcacca tcatgtagag
    10261 aaccctgttc tattcttaat actgttcctt ccatatagat ttttgaatta cacactagtt
    10321 taaactaaaa cacacaagga aaaacttacc atttaatcat aaaattagct catataaata
    10381 tactaggatg taaaatagac tggtcaaaat ctggtcaaaa aagaggacta atacaggtag
    10441 tc
//
LOCUS       DAYYHX010000095        10410 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000095 DAYYHX010000000
VERSION     DAYYHX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.096"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHA6Y_001745"
     CDS             6..305
                     /locus_tag="ACHA6Y_001745"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8987835.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHA6Y_001746"
     CDS             378..1739
                     /locus_tag="ACHA6Y_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM8987836.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHA6Y_001747"
     CDS             1761..2021
                     /locus_tag="ACHA6Y_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987837.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHA6Y_001748"
     CDS             2035..2787
                     /locus_tag="ACHA6Y_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM8987838.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHA6Y_001749"
     CDS             complement(2881..3486)
                     /locus_tag="ACHA6Y_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8987839.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHA6Y_001750"
     CDS             complement(3539..4513)
                     /locus_tag="ACHA6Y_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM8987840.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHA6Y_001751"
     CDS             complement(4891..5610)
                     /locus_tag="ACHA6Y_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8987841.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHA6Y_001752"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHA6Y_001752"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8987842.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHA6Y_001753"
     CDS             7161..8558
                     /locus_tag="ACHA6Y_001753"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8987843.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHA6Y_001754"
     CDS             8639..9079
                     /locus_tag="ACHA6Y_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8987844.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHA6Y_001755"
     CDS             9227..9553
                     /locus_tag="ACHA6Y_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8987845.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHA6Y_001756"
     CDS             9554..10303
                     /locus_tag="ACHA6Y_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM8987846.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3304 a         1834 c         2151 g         3121 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca
//
LOCUS       DAYYHX010000096        10397 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000096 DAYYHX010000000
VERSION     DAYYHX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.097"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..730
                     /locus_tag="ACHA6Y_001757"
     CDS             158..730
                     /locus_tag="ACHA6Y_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8987847.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            875..1579
                     /locus_tag="ACHA6Y_001758"
     CDS             875..1579
                     /locus_tag="ACHA6Y_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8987848.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1807..2145
                     /locus_tag="ACHA6Y_001759"
     CDS             1807..2145
                     /locus_tag="ACHA6Y_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987849.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2130..2984
                     /gene="lgt"
                     /locus_tag="ACHA6Y_001760"
     CDS             2130..2984
                     /gene="lgt"
                     /locus_tag="ACHA6Y_001760"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8987850.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3131..3364
                     /locus_tag="ACHA6Y_001761"
     CDS             3131..3364
                     /locus_tag="ACHA6Y_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM8987851.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3562..5466
                     /locus_tag="ACHA6Y_001762"
     CDS             3562..5466
                     /locus_tag="ACHA6Y_001762"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8987852.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5776..6138
                     /locus_tag="ACHA6Y_001763"
     CDS             5776..6138
                     /locus_tag="ACHA6Y_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFM8987853.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            6155..6766
                     /locus_tag="ACHA6Y_001764"
     CDS             6155..6766
                     /locus_tag="ACHA6Y_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFM8987854.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(6868..7776)
                     /locus_tag="ACHA6Y_001765"
     CDS             complement(6868..7776)
                     /locus_tag="ACHA6Y_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8987855.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(7818..8132)
                     /locus_tag="ACHA6Y_001766"
     CDS             complement(7818..8132)
                     /locus_tag="ACHA6Y_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8987856.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8431..10362
                     /locus_tag="ACHA6Y_001767"
                     /pseudo
     CDS             8431..10362
                     /locus_tag="ACHA6Y_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
BASE COUNT         3367 a         1714 c         2210 g         3106 t
ORIGIN      
        1 aatttaatag agaagcctgg cgtctattaa gaaataattt tcttgatgag cgtcaatttt
       61 tttaacggaa atctatccta tactaaattc agtaaagaaa aaggaggaat caaagaaagc
      121 tattttaaac aaaccaaaat tataggagga ataaatcatg actgaaaata aagctactca
      181 agaaagaaca ccccaagaaa gattgcaaga agaacaagaa cacaaggaac atgttcatca
      241 tacgaaaatt aatgcagcgg ccattgcgga tcatctttta ggcaacatgc atacattaca
      301 tgtgaaactg caccaatatc actggtatgt aaaaggagcc aatttcttta cgttgcatga
      361 aaaatttgaa gagttgtata acgaaaatga aaaatggttc gacaaacttg cggagcgtct
      421 aattacttct gggcataaac ctgcttcgac aacggttgaa tttgaaaaat attcgatgct
      481 ttcggaagat gcagttaata aatacgctaa agcagaagac atggttgaaa atattatcga
      541 ggacttcaga agcactcgtg acctaactat tcgcgcgatt cgtttagctc aagacgaggg
      601 tgacgatgct ttagaagata cattgattgc ttttaaaaat gacttagaca agaacatttg
      661 gatgctgcaa gcgttcattg gtaaagaagc attagaagat gatgacgctc ttgatgagga
      721 tgaagattaa ggtaaaagta gaagctgatt tagggattgg aattctttcc agtccttttt
      781 ccattttctt gatgtgcatc aatttttttg tagtaagaga cttatataat aaaagcagcg
      841 aatataaaca aaataaataa agggggagaa tgaattgact aaacataccc atcatgaaat
      901 tggcgaccac gcagcctgta tccgcttggt tccaatattc aatcacttag aggatagcgc
      961 gatgaattac attgctgaaa aagcccacac gaaacagtat caaaaaggag agtttctgtt
     1021 tcgttcacaa gaaaaagagg atactttata tattatcaat cgtgggaaaa tccgtattta
     1081 tcgattggca gactctggca aagagcagct agtgcggatc ttgaaccctg gtgatttcac
     1141 cggagaatgg accttgttca acccggatgc agtacacgag gaatatgccg aagcaacccg
     1201 agataccgtt gtctgtatga ttcagcaaaa agatgtccaa gattttctgg aacaataccc
     1261 agctatttct ttaaaactat tggcggaaat gtctaaacgg ttagaaagtt cagaacgtca
     1321 aacgacacaa gtagccgtgg agagtgtcat tacccgttta gttttgtttt tggcagaaaa
     1381 tgtggaacct gaaatgggca actctcccac catcaccctg ccgatggcaa aaaaggacat
     1441 tgcttcctat ttaggaacga cacctgagac catcagtcgc aaatttggag aattggagga
     1501 cgaaggattg attcaacagc tgtctgggaa aaggatcaag attcaggatt tagatgattt
     1561 attgctttac agcgaataat cggacacact tttttctaca cgttggtgtt ctgtcgctct
     1621 tcattaacaa cgggacagaa ctcagtgctt ttgcttccat ctgtccgttt ttggcttttt
     1681 taagacagaa cgaccttcat tcattggaaa aataaaagca caaacatgat aaccctcaag
     1741 tttttttgtt ttatttttct ttaagatagg gttgtcgaac aaaacgtaaa aataagtagg
     1801 gtgaatgtgc attgtttttg ggtgatagtt gccatcacgt tgtcagcagc gggggcattc
     1861 tttgtcgtcc ctttcaccaa gaacacgatt ggcggcgaag caaaaacgtc taacgttgtt
     1921 gaggcagata ggttagctat ggaaaaagaa gaggaagttg taaaaggagc cggcgtaatc
     1981 ggacacggaa aagtggcgta ttacgaagcg agtgaccagg gagataaaag tgtattcact
     2041 gtttggtttc catggagctt agccggaatc ttgttcagca tcaccatgac tagaatgacg
     2101 cgaaaaattg aagaaatgga gcaaatagaa tgggattcgt attaggcgtt ttgaatcccc
     2161 atgtggtgga gattggttct gttgtgattc attggtatgg agtcattatt gcagcgggca
     2221 tattagcggc tctccagttg agcaccaagg aagcaaagaa aaaaggactg gaagaggata
     2281 ccattatcga tatggcctta tgggcaattc cgattggact cctcggcgct cggctttatt
     2341 atgtgttatt tgaacttggt tattatctgc aaaatccagg acaaatcctt gcgatttgga
     2401 atggcggcat tgcgatttat gggggattga ttgcaggagg gggtacgcta tattggtatg
     2461 caaagaaaaa aggtacttct ttggcactcg ttcttgatat tttagcgccc aatgtattgt
     2521 tagcccagtc cattggtcgt tggggcaatt ttatgaatca ggaagctcat ggtgggccgg
     2581 ttactcgtca attcttggaa aatctttatt tacctgaatt tatcattgaa caaatgaata
     2641 tgaatggtac ctactaccat ccgacttttt tatacgagtc attgtggagt ttgttgggct
     2701 ttgtcctcat cgtcactata cggaaccgaa aacagctttt gcgtcaagga gaagtcgctt
     2761 tgagttacgt tttgtggtac tcagtgggtc ggttcttcat tgaaggtatg cgaacggaca
     2821 gtttatggat tggcgagtgg atccgcgttt cgcaagcctt gtctctggcc ctgtttattg
     2881 gcgcgattgt agtatggttt atacgcagac aagattatcc accaatttct tattattctg
     2941 atggcaataa ggggcagaaa aagactatta agatggtgaa ttgagcaaaa agataaaaag
     3001 aagtgcaaaa cttgatatgc gtcaattttc gtttgctaaa agtgagctat actacagttg
     3061 taaagaggaa aaggcagtgt tcattaatct gctgccgata aaataaaaac acataaagga
     3121 gataggaatt atggaaaaag ctatattgca attagaaacg ttgtcttgtc caagttgtat
     3181 gcaaaaaatt gaaggtgccg tgaaatcagt taacggtatt gataaagaca gcattaaagt
     3241 attattcaac tccagcaaag tcaaaaccaa ttttgattca gctgtcactt cgattgaaga
     3301 gattcaaaaa gctatcgaaa acgtaggcta tccggttctt aaagcaaaag tcaaggccgt
     3361 ttaacagatt aaatgaacta ggcaattctg gaagaaaagc tatttttcca ggattgcttt
     3421 ttttgttgca atccgattcg aggtgcttag aaacgataaa agagaacttt ctaaacttga
     3481 tgtcagtcaa ttaattactt aggcaattat cttatactgt gtttatcaat aaaagagaaa
     3541 ttaaatttgg agggaaaaag catgcaacaa tatatattaa gcaagaaaaa tgttatcacc
     3601 gtcatcagtg gattgttaat cgtactcggt ttcttcagtc actttgtttt agaaaacgta
     3661 ggactttcag agtggtcttt gatcatcgcc tctgtctttg ggattacgcc aattgccatt
     3721 caagcgtttc aagcaatgaa agtcaaagtc atcagtattg atgtcttagt cagtattgca
     3781 gcgattggtg cgctttttat tcaaaattat gaagaatcgg ctattgtcac gttcttattc
     3841 ttattcggac actacttgga acaacgaaca ttgaaccaaa cgcgatctgc tatcaaagaa
     3901 ttgactgaaa tggcacccga aagcgccttg aaacaaatgg ataatggcga atttgaagaa
     3961 gtagaagtgg atgatataga tgaaggggat atcttgctcg ttaaaacggg tgcgaaagtt
     4021 ccagtagatg gcatagtcct tacgggtgaa gggcatatca atgaagctag cattacaggt
     4081 gaagctattc cggtcaacaa gctagctgat gcagaagttt ttgcaggaac cattttagaa
     4141 aacggaacga ttcaaatcag agctgaccga gttggtgagg acaccacatt tggaaaaatt
     4201 attgaactcg tggaagaagc acaagattct aaatccgaag cggaacggtt cattgatcgc
     4261 ttttctaaat actacacacc agctgtcttg gttctggcaa ttgttgtatg ggcgttcagt
     4321 caaaatattg agttagcaat caccatcttg gttctaggtt gcccaggcgc attagttatc
     4381 ggagtgcctg tctcaaacgt tgccggtatt gggaatggtg ctcgtaacgg tgttctttta
     4441 aaagggagtg aagtcattca agacttcagt aatgtggata caattgtatt tgataagaca
     4501 ggtactttaa ctgtcggaaa cccaaccgtt gcagcgactg aactgtatga aaaagatcct
     4561 gctgaaacgc ttggctatct ggccagtgtg gaacgggaat cagatcatcc attagcaaaa
     4621 gcggtcttaa atcaaattgg agaaacaaac ttttatcctg ttgaaggaac tgaagttgtg
     4681 aaaggcggcg gaatcgtttc aagtgtagct ggacatagag tggctgttgg gaatgtggcc
     4741 ttgatggaaa aagaaaatgt cactctcagc aaaaaagtgc aaaaagatgt caaacggttt
     4801 gaacaacaag ggaactctct cgttttgaca gcggtcgacg gtgaattaaa agtactgatg
     4861 ggcattcgcg atcaagtccg tccgggtgtg aaagctaatt tgcaggaatt aaaagacttg
     4921 ggcgtgaaaa atctagtcgt tctttcagga gataaccaag gaaccgttga tgtggtcgcc
     4981 ggcgaacttg gtttgaccga agctcacggc tacatgttgc cggaagacaa atcggcttat
     5041 atcggaaaac ttcaacaacg tggtcaaatt gtagcctttg ttggagatgg cgttaacgat
     5101 agtccgtcct tagctttagc agacattgga atcgcaatgg gaagcggaac ggacgtagcg
     5161 attgaaacat ccgatgtcgt tttaatgaac tcgaacttca gcaacttgcc tcacgcatta
     5221 ggattagtaa aagccaccgc aaataatatg aaacaaaata tcgtgatttc aattggagta
     5281 gtgttggtct tgttgaccag cgtcttcttc agcgaatgga tgaatatgtc tatcggaatg
     5341 ttggttcatg aaggtagtat cttggtggta attttcaatg gcatgagatt aatgaagtat
     5401 aagttgaaag gtcgaaaaga acaaaaagaa gtatcagcac ctgcagagaa agtaaaaata
     5461 tcttaagaat aaattagaag aggctgagac aatagtccat ccttgaaatg acaaaagcgg
     5521 aaactcagat ttttaaatct gggattttcc gcttttttga gttcttgaaa aaaaataaga
     5581 gtatagagta tgttctttat aaaattaatt ttcgtgtaaa tgattaaaaa atttaataat
     5641 aaaatttaaa ttcctattaa gttgtaacac atgaaattgc ttataatagt gtattgaaat
     5701 taaggtagat tgacaaacga gtgatcaaat agatatacta ccttcaaacg accgtttgaa
     5761 tgtgaggtga gatgaatgga aaaagtttgc caagtgacta tcgtacataa agaaaaagta
     5821 aatgtaataa aaaaaaaatt ggatcaaaaa gatttttcaa acttacttgt gttagggaaa
     5881 tgtttcagcg attcttctag gattaaaatt ttctatgctt tagaaaccta taaggaaatg
     5941 tgtgtctgtg atttagcaga aatacttact gctagtgtcg ctacaacgtc acaccattta
     6001 cgttttttaa aaaaacatgg aatggcaaaa tcacgtcaag atggaaaagt agtttattat
     6061 tctttagcga atgaagacat cctttccgct attcgtgttt ttttaaagtt atcagaaaat
     6121 ctatctatga aatcttagtt tggaggaagt tattttgtta caaagtattt tttcagctat
     6181 cgctgtttat atttctacca gtattgatta tttgtttatc ttgctgatta tcttttctca
     6241 aagccacaca aaaaaaggta ttcgtcacat tttttggggt caatatcttg gaacaggtat
     6301 tttggtagcc gtaagtttgt ttgctgctta tgtgctaaat tttattccgc aagattggat
     6361 cattggtctt ctcggtctaa ttccgatttt cttaggaatc agagtcgctt ttgttggcga
     6421 agaggaggaa gaagaagagg aagtcgttga aaaactcgaa tctagaggaa ctaatcgctt
     6481 attctggaca gttgccttaa taacgattgc ttctggaggc gataatttag gtatttatat
     6541 tccatacttt acctcgttag ctttttcaga aatcattatt gctttaatcg tattcgctat
     6601 ctctgttgcg gttctctgct atatcagtta caaattagcc aagatttctt ttgtttccga
     6661 aacgttagaa aaatacgaac gaatcattgt ccctataatg tttattgcgt taggaatttt
     6721 tattatgata gagaacggga cgatacaaac gctactaggt ttttaaaaat cataatcacg
     6781 aaagataaaa agagaggcag cgactaatca aaatcgctgc cttttttgct tttcaaataa
     6841 tgaagtattt tttagcgtag aactatctta cgtgttgtgg gagtatccct tttcaagctc
     6901 ttctttgctt gtagttattt ctattgtgat atgattctct acgcttgatt tgtgtgcgac
     6961 ttctgcaatt tcttctttta tttccctagc cttgtctatg tctgaagggt ctataagaac
     7021 cgtgatgata agggcgtttt cttccccgtc cagtgaccaa atgtgaaaat gtgagaggtc
     7081 ttttacagca ggaacatttt taatggagtc cgccaagctc tttacgttta cattttcagg
     7141 cgaaccattc aataaaattt tcattgtggt gaaaaattca ggaacggttt tgtagaggat
     7201 ataaagtgca atcaaaatcg ataagatagg atccagacga tatgcctcgg taaagttcat
     7261 tagaatactc acaatcaaga ctccaatcca ccctaatacg tcttccagca tatggaggtt
     7321 caacatgctc tcgtttttgg atgtgccttt gcttaaaatc cacgcagcat atccattcaa
     7381 tgcaatagct acgagagaaa gccagaacat gccactataa tttacgggtt gtgggtcaat
     7441 caaacgtggc acactacgat agatcataaa aaacgagcca ctaattagca caacgccagt
     7501 tatcagcgcg cccagtaaag aaaagcggtt atagccaaaa gtgaaccgtt catcttgttt
     7561 tttctctgaa taaccttgaa aaaaccaagc gaatccaatt gaaatagagt cccctaaatc
     7621 atgaactgcg tcggacataa tggacacact attgaaaaga aagccaaaga aaaattcgct
     7681 tatagagaaa ataagattta tgaagaaagc aattttaata ttctttttcg gttgattctg
     7741 attcgagtga ttatggtctt tatctgattt tgacatttaa aaacctccta agtttgtatg
     7801 ctacatacta attggtttta tttttctaat tcattaacat gagttaagac ttgttcgaga
     7861 atactaaaaa catgaaggtc atctaaactg taaagcatac tttttccttc tcttcttgat
     7921 ttcactaagc gtgcgtcttt caacgttttt aattgatgag aaatagcgga ctgttccatg
     7981 tccagagaaa tagcgatatt tccaacacta cactcttttt tctgtaaaag aaagagaacc
     8041 gacaatcgtg taggatcact aatcacctta aatagtttgc ttattgacag aatagatcct
     8101 ttatcgattg gagaagttgt ttttatatcc atagtgtttt tattcctttc atatgacctt
     8161 ccgttcatat ctatattacc atatgagcga aacaatgcta atcttttgtt tataaaagag
     8221 aagtggcgtt ctctttatct tgacaaaatg tatatagcga tataaaatga gtatacaaac
     8281 atatgattaa ttagtcatat gtaaggtgga ttcattacga tttacttttg gaataaacga
     8341 aaggagtcag aaatagcaga aatttatgtg attcaagaag taaatgatgg aaaacaaagg
     8401 aattataaat tatataggga ggcaatagaa gtggaaacta tccaagaaca acagtctcaa
     8461 gaaaagcata atcataatca caaccacgat cacgaccatg atcatgggaa aatgccagtc
     8521 gttttgtact ttgttggttt agcattagca gtgattgctc tatttttgaa tgaagattat
     8581 cagttggtac aaaatgtttt gttctcaatc gctacaatca gtgccgggta tcacgttatt
     8641 cttcttgaag gccttggaga aacaattgag aattcaaaag cacaaaaaaa gttcatacct
     8701 aattctcata ttttaatggg attagcggca attggagctt ctttgatggg gaatttttgg
     8761 gaaggaacat tattgattct tattttttct ggcgcacatt ttttggaaga ttatgcagaa
     8821 ggaagaagca aacgagaaat aacgaaatta ctcgaaatga atccaacgac tgcccggtta
     8881 atcctgcctg atggtagtac aaaaaatgtg gatgtcagtg aattaaaagt gggagatcaa
     8941 cttcaagtgt tgaatggcga tcaagtacct attgatggcg tcattttgtc tggatctaca
     9001 tccattgatg agtcgtctat tactggagaa agtatcccga aagagaaatc caaaggagat
     9061 ggcgtcttcg ggagtacaat caatggaaca ggtaccttca cgatgaaagt caccaaagaa
     9121 aacaaagata cagtattttc aaaaatttta caattagtga atcagaacca agataaccaa
     9181 acaaaagctg cgagtattat ccaaaaattt gaaccgaaat atgtcacagt tgttttaatt
     9241 gccattcctc tattcatgtt attggctcct tttctacttg attggacatg gtcacaaagt
     9301 gtctatagag gattggtcct tttagttgca gcttcaccgt gtgctttagc agcagctact
     9361 gtctcggcaa cgctatcgac gacttctaat ttagccaaaa aaggagtcct ttcaaaaggt
     9421 agttcttacc tgtctcaatt agccgatatt aaagcaattg catttgataa gacgggcacc
     9481 ttgaccaaag gaaaacctga agtaaccaat tattattttg ctgattccgt gaacgaagaa
     9541 aacatgattg atatcgtagt agctcttgaa aaagaatcca atcacccctt agcagatgct
     9601 attctaagaa agtttgagca aaaaaacaaa ctaactattg aagtagaaaa ccaaattggt
     9661 aaaggattga cgggagatta caaagggaga aattatcgta taggaaaacc gacttcgttt
     9721 gatgatgttt cagatgaata tagtcgttta aataatgaat gggcgtcaga aggtaagaca
     9781 gtcgtatacg tagcagtaga tgaagaggtt ataggcctta ttgcccttat ggacgttccg
     9841 agtgaatatg ctaaagaaac cattgaatat tttaggaaac aaggtataca caccacatta
     9901 attactggtg actcagaaat gacgggaaaa gcggttgcta aacaattagg aatagatgaa
     9961 gtgattgcga atgtcatgcc ggaagacaaa tccagaatta tagacgaaca aaaagaaaaa
    10021 tacggcgtga ccgccatggt tggagacggt gtaaatgatg ctcctgctct tgttaatgca
    10081 gatgtgggaa tcgctatggg agatgggact gatgtggcag tagaggtatc tgatttagtt
    10141 ttaatgcaaa acaatttatc taaattggta caatctcata atatttcttc gaaaatgaac
    10201 cgtgttattt gacagaatat tattttttca atggccgttg ttgctttttt aattgtcgtt
    10261 agtttcttag gcttaacgga tattgcaatc agtgtcatta ttcatgaagg aagtactttg
    10321 gttgttattt tgaatggact tcgattgcta aaggcagtct aaagaatgat tgcacaactt
    10381 caaattaatc gagtaat
//
LOCUS       DAYYHX010000097        10159 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000097 DAYYHX010000000
VERSION     DAYYHX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.098"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            163..1038
                     /locus_tag="ACHA6Y_001768"
     CDS             163..1038
                     /locus_tag="ACHA6Y_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8987857.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            1052..1375
                     /locus_tag="ACHA6Y_001769"
     CDS             1052..1375
                     /locus_tag="ACHA6Y_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987858.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            1372..2124
                     /locus_tag="ACHA6Y_001770"
     CDS             1372..2124
                     /locus_tag="ACHA6Y_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM8987859.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            2114..4285
                     /locus_tag="ACHA6Y_001771"
                     /pseudo
     CDS             2114..4285
                     /locus_tag="ACHA6Y_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(4397..6568)
                     /gene="copB"
                     /locus_tag="ACHA6Y_001772"
     CDS             complement(4397..6568)
                     /gene="copB"
                     /locus_tag="ACHA6Y_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM8987860.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(6588..8774)
                     /locus_tag="ACHA6Y_001773"
     CDS             complement(6588..8774)
                     /locus_tag="ACHA6Y_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8987861.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(8774..8983)
                     /gene="copZ"
                     /locus_tag="ACHA6Y_001774"
     CDS             complement(8774..8983)
                     /gene="copZ"
                     /locus_tag="ACHA6Y_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM8987862.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(8996..9436)
                     /locus_tag="ACHA6Y_001775"
     CDS             complement(8996..9436)
                     /locus_tag="ACHA6Y_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM8987863.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(9510..10049)
                     /locus_tag="ACHA6Y_001776"
     CDS             complement(9510..10049)
                     /locus_tag="ACHA6Y_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8987864.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         2863 a         2157 c         1827 g         3312 t
ORIGIN      
        1 taaaaaaaca taataagatc actttgcttg taggatgtat tcccttccat tacaatgaaa
       61 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
      121 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcggcaa acgttcaact
      181 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
      241 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
      301 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
      361 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
      421 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
      481 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
      541 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
      601 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
      661 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
      721 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
      781 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
      841 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
      901 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
      961 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     1021 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     1081 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     1141 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     1201 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     1261 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     1321 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     1381 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     1441 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     1501 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     1561 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     1621 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     1681 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     1741 cttttttgct tttggggttc gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     1801 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     1861 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     1921 gcttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     1981 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     2041 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     2101 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     2161 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     2221 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     2281 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     2341 gacattttcc atattatgga tcttacagtt tttttcgtca gtcacagagg aattcttcca
     2401 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     2461 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     2521 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     2581 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     2641 ccggttgttg tcgggaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     2701 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     2761 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     2821 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     2881 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     2941 gaagatcgaa aataaaaatc attacaccgg ttctggatgg atagaagatc aggacgcacc
     3001 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     3061 caaagaaaac attacattga ccctaaaaaa acaagttttc ttaccttatc cttatggaaa
     3121 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     3181 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     3241 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     3301 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     3361 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     3421 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     3481 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     3541 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     3601 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     3661 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     3721 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     3781 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     3841 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     3901 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     3961 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     4021 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     4081 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     4141 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     4201 aaaaacctat ttagaaataa cgcatataat aagaaaaatg gtatacaaaa aaattctgac
     4261 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctggaatgcc
     4321 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     4381 caaccttttt tcattattat ctgattttca atgtcaaagc atttagagct acaactaccg
     4441 tacttaacga catcaagaca gcaccaactg ctggacttaa tatgatacca attggcgcaa
     4501 gaattccggc tgctagcgga attgcaagaa tattgtaacc tgccccccac cagaggttct
     4561 ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa atgcaaaata tcttttggat
     4621 cgctgtttgt caatacgaca tccgcagaat cgattgcgat atctgtccct gcaccgattg
     4681 ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt gatcccatca cccaccatga
     4741 ttactttttt cccttggtca aggtatttct ggacaacagc ttctttatca tctggtaata
     4801 aaccgccata atattcttcg atccctaaat aatctgctac tgcttgggca gcgtttttat
     4861 tgtcaccagt aagcatgaca ggagtgattt ttctttcttt caatgttcgg ataaattctt
     4921 ttgcttctgt ttttactacg tcacctaaag caacgattgc agctagatga ttttctaaaa
     4981 tcaggaagct gatggtattt ccttgttctt ggtaaggttt caataatgct tgttctacgt
     5041 ttaatccgag acgctcaact tctttttcat taactatttt cacttgttgt tttttgactg
     5101 ttgcctctag cccgactcct gaaagatttt ttagattttc agcttgataa ggcttgattt
     5161 catgttcttt caaataattc ataatgccaa cagccaatgg atgattggca ttcgcttcca
     5221 aggctcctag atattgaagg atttcatttt tattgaacgc atcatcaagt acttcgacac
     5281 ccgttacagt aaattttcct tctgttagtg ttcctgtttt gtccagcatg atcacatcta
     5341 gatcattggc ttgctccagt gcattgcggt ttttcaaaag taatccgttt ttagctgcta
     5401 ttgatgtgga acgagcaaca actaatggga tagctaaacc tagagcatga ggacaggcga
     5461 tgataaagac tgttaccata cgttctaatg cattaggaag atctgtcaag aacaaccagg
     5521 cgatgaatgc caggataccc acaattaatg cgacataaaa cagccatttt gccactttgt
     5581 cagataatga ctctagcttg gatttttctc cttgtgcttt tcgcaccatt tccataacct
     5641 tcgctagata accattttca ccagttccag ttacttcgat ttcgatcgtt ccatccccat
     5701 tgatagaacc gccaatcact ttgtcattgg cttgtttttt gattcctctt gattcgccag
     5761 tcacagctga ttcgtcaaca attgtttcac ctttcaagat tacaccatct gttggcattt
     5821 tatcaccaga ccggacgatc aaacgatcac cttcgtttac ttctttcaat gaaactgtct
     5881 cttcttttcc atctttcgtc aagcgtttca ctgattctgg cagtaattct gctagttttt
     5941 gtaaagcatt gctggcattc gacacagcat tcatttcaat ccagtgacct agtagcataa
     6001 taacgatcaa agtagccaat tcccagaaga aatccatgac gtgtgtatgt ggattgagca
     6061 gatttgcgat aaacgaatag acactataaa tatatgcaac tgtaatcccc atcgcgatca
     6121 gtgtcatcat agcaggactt ttttgtttca attccatctt agctccactt aaaaatggct
     6181 gaccgccata gataaatagt atagttgcta agatcaatac gagccaatca gaaccaggaa
     6241 acgttacttg aaatggaaat tccattccca tcattggtga aaaaagaatg attgggatag
     6301 ctaggataag cgatagccaa aatttttgtt tgaaattccc catgtgcatg gaatgatcca
     6361 tgccgccgtg ttcgtgatcc atgtgcatcg aatggtccat ttgtttgctg tttgctatat
     6421 ctccatggtt atgtcccatg tgcatggaat gatccatctg actatggtca tgtttcatct
     6481 ccatctgttt ttcatgagat tctgtacttt ctttgtttac tacttctttt ttatgctcat
     6541 gttttttctc catatcgttc ccattcattt ttactccatc ccttctctta ctttttcatg
     6601 tgtcgattta agcacaggga gttcaacaat acactgacgg aactaaacgc catcgctcct
     6661 cctgcaatga ttggattcaa aaagcctagt gcggcaaaag gaataccaat cgtattatag
     6721 acaaaagccc agaaaagatt ttgtttgatt tttctcaatg tcaacctaga caactcgatt
     6781 gtttgtgcaa tggatgccaa actgctattc atcagcgtca catcggctgt ttccatcgca
     6841 atgtccgttc cacttcccat tgccataccg acatcagcta atgctaaggc cggtgcatca
     6901 ttgattccat cgcctgccat accgactttt ttgccatctt tctgaagttt ttctacgtag
     6961 gctgctttat cctctggaag tacttcagca aagatatgct ttggatcaat accaacttgt
     7021 ttaccaatcg tttcggctgc cagtttgttg tctcctgtca gcataaaaac atctaatcct
     7081 ttattctgga gctgcttgat tgcttgtttg acttctagtt tgatttgatc agccacggca
     7141 atcaacccga tcacttgctt ttcatcagct aaaaacatga ctgtttttcc ctgctgttct
     7201 aattctaacg cgtgttcttg gtattcatca aatgataggt tcaattcaat taatcgttta
     7261 cgtgtacccg caaaatagcg tactccgtta atcgtaccgc tgattccagc acctgggtgg
     7321 gctgtaaaat caatcatatc gtatgttgct gcttcttgca gttttccgta ttccacgatt
     7381 gctttaccta gtggatgttc agaagcttgt tctaaagtat aaaaaataga taggacttct
     7441 ttagaaccga ctagatctgt aactttgggc tttccttctg taatcgttcc agttttatcc
     7501 aaaacaatgc tgtctaagtg agcagctgct tctaatgctt ctccgccttt aattaaaatt
     7561 ccatttcgtg caccaacacc cgttcctacc ataatagctg ttggagtcgc taaacctaat
     7621 gcacatggac acgcaatgac taatacagag acactatgga tcaatgcgag ttgccaatca
     7681 ccagtgatca aacctgtagc tatgagcgtg ataaacgcga tgactaatac gatcggaaca
     7741 aaaatccctg agatccgatc tgcgattttt tggattgggg ctttgctccc ttgagcatct
     7801 tccaccattt gaatgatttg cgctaatact gtctgcttgc cgacttgtgt aacttccatg
     7861 tgaagtagac cattcgtatt gattgtcccg ccaaagagcg tgtcatccgg attcttttct
     7921 acaggaagat tttcaccagt aagcatactt tcatctatcg cactgcttcc tgaaataatt
     7981 cgaccatctg ctggaatctg ctcaccagga cggatgacta gttgatcacc aacgactact
     8041 tcttcaatcg ctaacgtttc ttctttcccg tttcttatca cttgggcggt ttttgtttga
     8101 agagacatca attgtttgat tgcattacct gttttcgttt ttgctgtatg ttccaaatat
     8161 ttgccgagca aaatcagcgt gatgatcata ctgctgcttt caaaataaag atcttggggg
     8221 tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg cagccgatgt tccaattgct
     8281 accaatacat ccatatttgg tgctttcgtc ttgattgcat ggtacgcacc tttataaaaa
     8341 cgagcaccga cataaaattg gacaggagca gacagcacta actggacgat tggaaaatga
     8401 aaaaagtgaa caatcgccgc atggcttcct aacatcatag caatcattga caacatcaac
     8461 ggcaaagtca aaaccgtact caatataaga tcgaatagca tttttttcaa atacgcttgc
     8521 ttttcttctg caattttttg tttgtgggct tcatcgtaca aaattgcacc atagccgatg
     8581 ttttctacgc tttggattaa tttttcagtg gtcgtccctt catatttgac acttgctttt
     8641 tcagtggcca agttgactgt ggcattgacc acaccaggtt gtccgttcag ctctttttcg
     8701 atcctagcag aacaatttgc acaagtcata cctgtaatga cgaacgtatc cattttggtt
     8761 tctgctgttg ccatcaaatc acctctgctt tataacccaa tccattgatt gcttgacaaa
     8821 tcttttcgga ttgaactttt gtttcatcaa acttgaccgt accgtttgct tttttcaaat
     8881 ttactttcac tttttgcacg ccgtcaagtg cgcttactgt ttcttctact cttgctgcac
     8941 aatgattgca gctcatacct tcgattgaaa attgttgttt cattttttct tcctcctatt
     9001 gatgttgttt acattcacat tgacctggga tacagttaca ttcgatcgta tctactggtt
     9061 gcttttgttc cagttgtttc aaaatctcat tgatatcttc ttgagtcaaa gtggcttctt
     9121 ctactagatc cgcaatcgtc tctccaatcc tttttgcaca gatatgggaa aaaagatttt
     9181 ccgtcgcact gcgaactgtt tccatctccg agacagctgg ataataaatg aatttttttc
     9241 cttcttgttc tgtccagatc acttcttttt ttaccaaacg acccagcaaa gttttgatcg
     9301 ttgctggttt ccaatccatc gaatcagcta agatctgcgt gatttcttgg gctgtcgtct
     9361 gtccaagtgt ccaaataaca cgcatgatct cccactctga atcactgatc tttactggcg
     9421 ttttgacttt ttccatttat ttcacacctt tcgtttacaa atgtaatcat tatcttatga
     9481 atacagatta cacttgtaaa caaaaactgt caattatttt tgctctctat tttcaattta
     9541 gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga tcagtttatt tcttgcatta
     9601 gctacttgga tcagctgctg tattttcggt tcattgtttt cttcaaaatg ccagataaaa
     9661 gataaaaatt ctaagtattc cctgtttagt ttctccgaaa atgattcagg catatctggc
     9721 cgagattttt tgtttatctt tctttttaga gaacgccaaa ctactcggta gatggcgaga
     9781 taacgcggca ctttaagcca aatgatctta tctgcttctt taatcctttc ctctaatgta
     9841 gaagtataat ttccttcaac gatccatgac ggattagata ccataaacaa ttgctgttct
     9901 tgcaaaaacc acttttttgc ctgaatactg tagtctgttg tatgccataa aaggtctagc
     9961 ggcagtagcg gaaagtcggt gatttctttg atcttccttg cgagggtcga ttttcccgac
    10021 ccactttgtc caatcacgat gatcttcatt cttctccctc acttctcttt ggaatttggt
    10081 tcgatcatac cacttataac gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa
    10141 agtcccttgt ttcgaataa
//
LOCUS       DAYYHX010000098        10090 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000098 DAYYHX010000000
VERSION     DAYYHX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.099"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(115..2235)
                     /locus_tag="ACHA6Y_001777"
     CDS             complement(115..2235)
                     /locus_tag="ACHA6Y_001777"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8987865.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2465..3643
                     /locus_tag="ACHA6Y_001778"
     CDS             2465..3643
                     /locus_tag="ACHA6Y_001778"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM8987866.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3659..4417
                     /gene="aroD"
                     /locus_tag="ACHA6Y_001779"
     CDS             3659..4417
                     /gene="aroD"
                     /locus_tag="ACHA6Y_001779"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM8987867.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4440..4925
                     /gene="ybaK"
                     /locus_tag="ACHA6Y_001780"
     CDS             4440..4925
                     /gene="ybaK"
                     /locus_tag="ACHA6Y_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM8987868.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4996..6249)
                     /locus_tag="ACHA6Y_001781"
     CDS             complement(4996..6249)
                     /locus_tag="ACHA6Y_001781"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8987869.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6366..7715
                     /gene="gdhA"
                     /locus_tag="ACHA6Y_001782"
     CDS             6366..7715
                     /gene="gdhA"
                     /locus_tag="ACHA6Y_001782"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM8987870.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7827..9173
                     /locus_tag="ACHA6Y_001783"
     CDS             7827..9173
                     /locus_tag="ACHA6Y_001783"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM8987871.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9481..9867)
                     /locus_tag="ACHA6Y_001784"
     CDS             complement(9481..9867)
                     /locus_tag="ACHA6Y_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8987872.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(9916..>10090)
                     /locus_tag="ACHA6Y_001785"
     CDS             complement(9916..>10090)
                     /locus_tag="ACHA6Y_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM8987873.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         3219 a         1839 c         2054 g         2978 t
ORIGIN      
        1 aaaaatctga gccagaatga ggttcattct gacccagatt tgattttgta caacccatca
       61 gtccttattg acaaagagcc taattttgtc gctgctcctt ttaaatttat tttatcattc
      121 ggaagaactt gtggtcgttc cgtgaagttc ttggtacgtt tgcgttttat aaagatcaac
      181 agttgattgt ccgttgtgtt cactaaagac actagttgac ttgtcaccaa gagatttttc
      241 agcttctttt aatttctcta agccattctt gtagtcataa tcactggtat caattgggtc
      301 taatccacta ttcgtataga accgaagaag atcaccatta ttgatttggt cagaaacagc
      361 taactgttta tcaacttttt ctttcagtga atcaatttca ttctgtactt cctcagtagg
      421 ttcagtaata gcttcaccag tttcgttact atagatcgta ccaccgtagt aagtatattt
      481 tggtgtaaca aaatcaccat cacggaaaga aacaagctgt tcatgatctt tcgagaatag
      541 atcttgtcct aactgaatat aattttttgt atctacacct agtaagtgaa gtaatgtagg
      601 caaggcatca acttgtccgc cgtaagtgtg attgattccg ccctgtgttt gtccagggat
      661 atggatcata taagggacac gctgcatttg tgcattgtcg tagtttgtcc aagtagaact
      721 tgtttttcca actaattcag ctagattttt atttcttgaa ttcgagacac cgtaatggtc
      781 accgtaaagg acgatgactg aattatcata caagccgctt tctttcaaat aattgaagaa
      841 ttcttgtacg gcggtatcta aataattggc tgtcgcaaag taaccgttga tcgtttcatc
      901 atttgtattg gctaacggga atcctgcttc gtcattggtg aattttgaat aaggataatg
      961 gttagataca gcaatgaatt ttgaataaaa tggttgttgc agacgttcca agtattgtac
     1021 ggattgttgg aaaaatggtt tatcatgcaa cccgtattgg aacgaattgt catcattaac
     1081 atcataataa gaagaatcaa agaaataatc atatcctaaa ttcttgtatg tctcgtttcg
     1141 attccagaat gtaccagcgt tcccatggaa taccgcacta gtgtaacctt gtgtttgact
     1201 taaaatatca ggtgcagctt ggaaagtatt ttttccgcct aattgtgtga acaaggagcc
     1261 ttgattcaag ccaaacaatg aattttccat cagcgtttcc gcatcgcttg tttttcctgc
     1321 ttttacttga tggaaaaagt tgtcaaaact gaacgtgctg ttgctgtggt aaagactatt
     1381 gatgaaaggt gtaacttcat gctggagacc attctcatct gtcaatttat aatcgatcag
     1441 gaattgctgg aagctttcta aatggatata aatcacattt tttcctctag cgagcccgaa
     1501 caaatcgtca tttggtgctg cataatgttc ttttacataa ttttctactt ctttcatatc
     1561 attgggactt gcttctgccc gcacttggtt tgtctggtaa gtttgaacac cgtcataaac
     1621 catgaaagca ttcaagccaa gatatttgac caaataatca cgagagaatg ttcgagtcag
     1681 caattctgga cgatcagctt ccgccataaa aagatttcct gagaaaatca taactgctaa
     1741 tgtagagatc gcaaaagcca ttcttgctcg gatcgggcga gggtccaact tgattttttt
     1801 tgcaatcagc agaacagtca agataataaa atcgatccaa tagatcacat catatgggcg
     1861 aaacagtcga atggcacttt ctcccagacc actcgctact ttcccagcac ctaaaatcgt
     1921 gttgatcgta atgaagtccg tgaattctcg ataataagtg acattcgaaa aaacaagcaa
     1981 tgtcaaaaga aaataaatga ccatcatcgt gatataagca actctagggc gtctgatata
     2041 aagagcaatt ccaagtaata acaatgtagt tgcaataggg ttgataaaca agataaagta
     2101 ttgaatgcca ctttcgattc cgagatggaa atcaactaaa taggcaaaaa tcgttttcaa
     2161 ccaaagtaaa acgactagta aagaaaaaaa tccgagcctc ttattcaaaa aggcaggtgt
     2221 ttttatacat ttcaagataa tcttcctttc tgcacttctt aatcacagaa gtgtcctgaa
     2281 accatgttac acagctcact tattctcatt tattttacta gtatgacaat atagtgtcaa
     2341 ttgaaaccag aagtttatgc tgtttcatta aggttttctt taagcttcat gaaaaaatga
     2401 gcagtaagac aagtcctatg ttttttgtta tacttgggaa gagaaagcta aagcaggtga
     2461 aggaatgaaa gtaaaagtaa aaaaaagagc aagcaatcga ttgaaaaaag gatatccact
     2521 gatccaaaaa gaagaccttg ctcatcccca aaaagaaacc acgcaatggg tggaattagt
     2581 tgattcgcaa aaccaattta tcgcaaaagg atatctagga aagcagaata agggaatcgg
     2641 ttgggtattg agccagcaga acatcccatt tgatcaatct ttttttgaag aattttttgt
     2701 gaagtcaaaa gaaaaaagaa ctttcttttt ttcagatgat tctactaccg cttttcgtat
     2761 attcaatgga gaaggagacg ggatcggcgg tctgattgtt gattattatg caggttatgc
     2821 tgttttttct tggtataacg aaacgttata tgcacatcgt gaattattga ttcaagcttt
     2881 tcgtgaaatc tacccggaaa tcataggagg ctatgagaaa attcgatttg aatcagtaaa
     2941 tctgcctgag agccagttca tttacggaaa agaggcaccg gaacccttga tcgtaaaaga
     3001 aaacggagta aaatttgccg tttatttaaa tgaaggatta atgactggga tctttttaga
     3061 ccaaaaagaa gtacggggat cattagcaga aggtttcgct tctgggaaaa cagttttgaa
     3121 catgtttagt tataccggcg ccttttctgt tgctgccgca atgggagggg caatcgaaac
     3181 aacaagtgtc gatctagcaa aacgcagttt aaaaaaaaca caggaacagt ttgaagtgaa
     3241 tggtttggat gtcgatcagc aaaaaatcat tgttatggac gtattcgagt atttcaaata
     3301 cgccaaaaga aaacaatttt tatatgacat gattgtattg gatccaccaa gttttgccag
     3361 aaataagaaa aaagtctttc gcgtagcaaa agattatggg gaactggtca aggatagtct
     3421 ggatatttta gcagaagacg ggatacttat tgcgtctaca aatgctgcga atgtctcgat
     3481 agaacaattc caaacaatga ttgaagagga attccatgcc aaaaatgttt cttttgaaca
     3541 acttgcttta tatcgtctgc caaaagattt ccagacgacg gatacctttt tagaaggcaa
     3601 ttatctgaaa gtgtttgttt atcaaattaa aaataaccat tagaaggagc gatgcagaat
     3661 ggttcgggta aagggattga atataggaag cggtagacca aagatttgtg cgcctcttat
     3721 cggggccgtc cgagaggaag tattacgtga agcagttctt gcacgtgaag caggggccga
     3781 tttagtcgaa tggcgagtcg atcattatcg tgaagttttt tcttttgatg aagtagcaga
     3841 aacattggtt cttatccatg agatgcttga tggactgccg ttattattta cgtttcgtac
     3901 cttagctgaa ggcggagaac aagacatatc gatccgtcag tatcgcgacc tttatgaaca
     3961 tgtgatacac acaggattag tagatatggt cgatatcgag ttgttcaaag tagaaagtct
     4021 aggaaaaaga ctgatagagg aaatcaagtc tttaaacatt cctttaatta tcagtagtca
     4081 tgattttaaa gaaacaccag cagaccctgt cttgctttac cgattgaatg ttatggaaca
     4141 ttttggagcc gatatcggaa aactggcagt aactcccaat aatgaacgag atgttttgcg
     4201 gttgatggag ttgactagac gggcaaatgc ttttgtctcc atgcctatta ttacgatgtc
     4261 catgggaaat cttggtaaaa tttctcgtct ggttggcgga acgacgggtt ccgttatgac
     4321 atttggttcc ttaagcagcg attctacttc tgctccagga caattaccgg tcaaagcatt
     4381 gaagcaaatt attcaattat taaatgaacc gttgtagaac aagtttgagg tgatattttt
     4441 tggcaaagaa aaaaatagta aaaacgaatg caatccgtat ggtcgaacaa aaaaagattc
     4501 cctatacaga gcacgaatat gagtgggacg aaagccacct aagtgcgtca tcggtcgctg
     4561 aacagcttcc agaaagccaa tctcgtatat ttaaaacact ggttgctgtg ggcaatgtga
     4621 cggggcctct tgttgctgtg ataccgggag aagccgaatt gaatctaaaa aaattagcaa
     4681 aagtttcagg gaataaaaaa gtagaaatgc tccatttaaa agatttggaa gcaacgaccg
     4741 gatacatacg tggtggctgc tcgcctatag gtatgaaaaa actatttcct acttatttag
     4801 atcaaattgc agaaagctat gaacaaatca ttgtctcggc tggcagaaga gggctacaga
     4861 tggagttagc acctcaagat atctgtgcat taacgagcgg acaatttgca gatataaaac
     4921 aataaatcat acgtataaaa gaaagaagga ggccgggatg ttgtttgtcc agtctccttc
     4981 tttctttttt atacattatg ctctttgccc ggcattcatt cctgctacat gccctgtagc
     5041 gaaagccgct gtgatattaa agccgccagt ataaccattg acatccagta attctcccgc
     5101 aaaatacaga ccatcaatga ttttactctc caatgtttta ggataaattt cctttagaga
     5161 aacccctccg ccagttacaa atgatttctc aataggaaaa gttttgttga ttgatagctt
     5221 aaactctttg catagttgaa caaaatcttg aatttgtttt tgagaggctt gttgtcctgt
     5281 cagatgatcc atttcaagcc gctcaagaaa gaaaactaac aatcgctcag gcaatatacc
     5341 atgccaagca ttgaccaagt tctttttggt tgctttactt ttttctgtca atatatgaat
     5401 aagctcttgt ttggtttgtg tagggaaaca atccagagaa agtgtcactg gttctcccgt
     5461 tttctctaat tctttattga caaaacttga acaacgcagt gcagccggac cagaaatacc
     5521 gaaatgggtg aaaagtaaat ccatcgtatg ttcagtcaat acacgacctt ttttatttaa
     5581 aacgcgtaaa gtgatgtctt gtaaagaaag cccttgaagt gttttctcct gaatatacgg
     5641 ttcatccgat atcaaggggg actctgtcgc ataaagaggc gtcaccgtgt gacccactcg
     5701 cttcacgatc ttgtacccat ctccagtaga accagtagat ggataagtcc gtccaccagt
     5761 tgttaagata atggcaggag catggaactc ttcgaattcc gtgcgtatac cgtaaatttt
     5821 atgttctttg tggatcaatt tctctactcg agtagagaat agaagcgtca catctagttc
     5881 tttcagacga ttgagcaatg cttcgataat cgtttttgat ttatttgtta caggaaacat
     5941 ccgtccatga tcctcttctt ttaaatgtac gccttgtgat tcaaagaagt tcatgatatc
     6001 gaaattattc cattgcgcaa acgtactata taaaaacttt ccatttccag gaatatgtgc
     6061 aatcaaatca tcaacaggac gattgttcgt aacattgcac cgaccaccac ctgtcatcag
     6121 tagttttttt cctgcttttt tgtttttttc gatcaagagt acgcgtgcgc cttgttcagc
     6181 tgcactgatc gctgccatca tcccgcttgt tccagcgccg actacgatca catcatacag
     6241 tttttccatt ccctcacctc aaggaaagtg tagcatatct gaagaaagaa aacagaaaaa
     6301 aatgttttga gccaaaagag aaaatgagct aaaatgaggg aagataagca aaggagcgtg
     6361 gaacgatgac aaacgcaaca gaatatgttc aagcaattca agaaaagcta catcaaaagg
     6421 atcaagggca agaagaattt ttacaagcaa tcgatgaatt catgcccacg gttatgtctt
     6481 ttttggacac tcatcccgaa tatatcgaaa agaatatctt agaactttta acggaaccag
     6541 aaagagttat tcaatttcga gtgccttggc aagatgatga aggaaactgg cgagtaaata
     6601 gagggtaccg tatccaatac aactcagcta tcggccctta taaaggtgga ttgcgcttcc
     6661 atcctagtgt caatctaagt atcttgaaat ttttggcttt tgaacagatt ttcaagaata
     6721 gtttaaccgg tctgccaatc ggtggaggaa aaggcggaag tgacttcgat cctaaaggaa
     6781 aatctgataa tgagatcatg cgtttttgtc aaagtttcat gctggaattg tcaaaacata
     6841 tcggcccatc gattgatgtt cctgcgggag atatcggtgt aggagctaga gaaatcggct
     6901 atttgttcgg cgaatataaa cgattgaaaa aatatgatac tggtgtgttg acaggtaaac
     6961 ctttggattt ttggggaagt aagatacgta cggaagctac tggttatgga ttagtgtatt
     7021 atgtcaaaca tctattgaac gaagaaaaag attcatttaa tcaaaaaaca gtttttgttt
     7081 ccggaagcgg gaatgtagcc atttatgcaa tcgaaaaagt gcaagagcta ggcgggaaag
     7141 ttgttacatg ttcagattct agcgggtaca tctatgatcc agaaggaata gatgtgaaac
     7201 tattgaaaga agtcaaagaa atcaatagac aacgtttgac ggaatacgca aaccaacgtc
     7261 cttctgctat ttatcatgct ggagaatctg tttggacatt gaaagaaaaa gcggatatcg
     7321 ctttgccatg tgctactcaa aatgaaatag acgaaagctt ggccgaaatc ttagtagaaa
     7381 atgggatcaa aatcgtagca gaaggcgcta atatgccatg tacagtaaaa gctgtttctg
     7441 ttcttcatga agcaggcgtg tggtattgtc caggaaaagc tgctaatgct ggaggagtag
     7501 cagtatctgc tttagaaatg agtcagaacg tccagcgatt gacttgggaa agagagcaag
     7561 tggatgagca gctggatcaa atcatggaaa cgatttacca tacgtgtcgc caaacagcag
     7621 ccgagtacgg cgacgaaaaa aatctgcttc ttggagcaaa tgttgctgga tttgaaaaag
     7681 ttgccaaagc aatggcagca gaaggtttag tttgaaaaat gaaactttct tggaaatatt
     7741 gtcagcattt tgtttatttg cttgaaaaat aataaaaaaa aacgtaaagt attacctgta
     7801 gagtaaacct ataaggagga gaacaaatgg cacacattca ttttgactat tcaaaagtag
     7861 caccgtttgt caacgaacaa gagttagggt acatgcaaag ccaagtaaca gcagcccaca
     7921 acgaattacg ggaaggtact ggcgcaggaa gcgacttccg cggctggatc gatttaccaa
     7981 cgaactatga caaagaagaa tttgcacgta tcaaagaagc agctaaaaaa atccaatctg
     8041 attctgaagt attagttgtt atcggtatcg gtggttctta tttaggcgct cgtgcagcga
     8101 tcgacttctt gaatcacaca ttcttcaatt tattagatag caacacaaga aaagcaccac
     8161 aaatcttctt tgccggaaat tcaatcagct caacatatat tgctgacttg atcgaagtaa
     8221 tcggtgatcg cgacttttca gtaaacgtta tttcaaaatc tggaacaact actgaaccag
     8281 cgattgcgtt ccgtgtattc aaagagcttc ttgttaagaa atatggacaa gaagaagcaa
     8341 acaaacgtat ctacgctaca acagataaag caaaaggtgc agttaaagta gaagcagatg
     8401 cagaaggttg ggaaactttc gtgattcctg atgacatcgg cggacgtttc tctgtattaa
     8461 ctgctgttgg tttgttacca atcgctgtta gtggtgcaga tatcgatgca ttgatgcaag
     8521 gtgccgctga tgcaagcaaa gcatactcaa gcgacaaact agaagaaaac gaagcttacc
     8581 aatacgcagc aatgcgcaac attctttacc gtaaaggcaa agtaactgag ttgttgatca
     8641 actatgaacc aggaatgcaa tacttctcag aatggtggaa acaattgtat ggtgaatctg
     8701 aaggaaaaga ccaaaaaggc atctatcctt caagcgcaaa cttctcaact gatttgcact
     8761 ctttaggaca attcatccaa gaaggacgtc gcaatatttt tgaaacagtt gtgaaggttg
     8821 aaaaacctcg taaatcaatc acaattcctg aacaagcaga agatttagac ggtctaggct
     8881 acttgcaagg aaaagaagtg gacttcgtaa atacaaaagc atttgaagga acattgcttg
     8941 ctcatacaga cggtgacgtg cctaacttgc tagtcaaaat tccagaaatg gatgcttata
     9001 cattgggtta tacaatgtac ttctttgaaa ttgctgtagg tatttcaggt tacttgaatg
     9061 gaatcaaccc attcgaccaa ccaggtgtag aagcttacaa gaaaaacatg ttcgcacttc
     9121 taggcaaacc aggctttgaa gatcttgcta aagaattgaa cgaacgcctg taagataagg
     9181 gtttttcgct agatttaaat aatcagtgat agaaacatgt tcgctttaaa atgaaaactg
     9241 gtacccattt aggtactcaa acatactgca tcgtttattc agaataaggg gctgtgacaa
     9301 gacgtttgtc acagtccctt gtttgtatag aaatcgattg attgccttgc tctttagttt
     9361 gttttaggac tttaagaaca gtgaggctga cttcgaaggt taattctgtt tttttgtttt
     9421 ctagaatcag taagcaaaaa agcactcact tcgagtgctt ttttgcgata tattcttagc
     9481 ttaagatagg cctaattttt cttttacaga tgagggtacc ttatcgctgg taattacttt
     9541 tttctctatg atccgctttt cgcttgcttt caactgcaag atcgttcctt cggggtaagc
     9601 agaaccattg gcagagtcac cagaagtaat gacatcgaaa tctaactgtc ttttttcacc
     9661 gttttcagca taagcatcta cgttgtattt aaaacctctt ccaattactt ccccattatc
     9721 tgctttgata tcagtttctg caggcattgg agctttgact accgcataat aatctttacc
     9781 tacataagta gactgatagt agtcccaacc tttccatcct ataaaagcaa tcaatatgat
     9841 aacgatcaac cctaaaattt ttttcatgaa agatctcctt ttttattttt attatacccg
     9901 tggtgctagt taattttaat atcctaaaga atatctcaaa gttccttcaa aacgtcgttg
     9961 tgctcgactt aatagaacac tgtaagccag taatatgctt tcaaatctac tctctagccc
    10021 tttaacagaa cgtgagttga aattttgaca acctaatttt tctaaggaag aaaaaacagt
    10081 ctcaactgtt
//
LOCUS       DAYYHX010000099         9957 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000099 DAYYHX010000000
VERSION     DAYYHX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.100"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..550)
                     /locus_tag="ACHA6Y_001786"
     CDS             complement(38..550)
                     /locus_tag="ACHA6Y_001786"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM8987874.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(564..2861)
                     /gene="recJ"
                     /locus_tag="ACHA6Y_001787"
     CDS             complement(564..2861)
                     /gene="recJ"
                     /locus_tag="ACHA6Y_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM8987875.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3003..3446)
                     /locus_tag="ACHA6Y_001788"
     CDS             complement(3003..3446)
                     /locus_tag="ACHA6Y_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM8987876.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3447..4235)
                     /locus_tag="ACHA6Y_001789"
     CDS             complement(3447..4235)
                     /locus_tag="ACHA6Y_001789"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8987877.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4232..5173)
                     /gene="rnz"
                     /locus_tag="ACHA6Y_001790"
     CDS             complement(4232..5173)
                     /gene="rnz"
                     /locus_tag="ACHA6Y_001790"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM8987878.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5350..5937)
                     /locus_tag="ACHA6Y_001791"
     CDS             complement(5350..5937)
                     /locus_tag="ACHA6Y_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM8987879.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6158..7471)
                     /gene="obgE"
                     /locus_tag="ACHA6Y_001792"
     CDS             complement(6158..7471)
                     /gene="obgE"
                     /locus_tag="ACHA6Y_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM8987880.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7759..8769
                     /gene="gap"
                     /locus_tag="ACHA6Y_001793"
     CDS             7759..8769
                     /gene="gap"
                     /locus_tag="ACHA6Y_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8987881.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8854..9789)
                     /locus_tag="ACHA6Y_001794"
     CDS             complement(8854..9789)
                     /locus_tag="ACHA6Y_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8987882.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2753 a         2274 c         1719 g         3211 t
ORIGIN      
        1 tttaagaata acatcaagaa tagcttcttt tttctgctta atattccatt aatgtaatga
       61 tgtcatatcc tttgattttg tcgcgtccat ggagatccat caattcgatc aaaaatgcgc
      121 agcctacgac tacaccgcct aattgttcga tcagttcgat cgtcgctttg attgttcctc
      181 cagtagctag caaatcgtca caaatcagca cacgttgacc tggtttaatg gcatccttat
      241 gcaatgtcaa tgtatctgtt ccatattcta atccataagt cacttcgatg gtttctcgag
      301 gtaacttccc ttttttgcgg actggggcaa aaccgactcc taactcataa gcaacaggac
      361 atcctacgat gaatccacgg gcttctggcc caactaccat atcaattcgt ttttcttttg
      421 cataatttac gatttgttta gtagcttccc gataagcgtc accatctgcc atcagtgggg
      481 aaatatctcg aaacacaatt ccttttgacg gatagtcggg aatactggca atataatctt
      541 ttaaatccac tctttgtttc ctcctaggtt gttaaccatt tctttatggt aggaatatcg
      601 ctcaaaagca aaaattcctc aactttgatt ttattgagcc gtcgctggta taatctgctc
      661 tcagtcaaag gatgactttc tgggctagcg actttttgta aaacaccgtt ctctattgta
      721 acaaatttca attcaaaaaa cacctgtatc ataaaaatta ataatttttc agggatacgc
      781 aaatattgag ctattgcttt tattttatga cgtatatcaa ttcgttcttg agaatggatc
      841 aatttaaaca attttgcgta ctgttctctt gtgcctaccc cattcaaata ggcctcatca
      901 ggtgaaatcc ccatcagata aacacgattg aatgaataag accgaaaaac ttctttcaga
      961 tccgctaaat catctggaca atccataaca actagagagt ttggttttct tttttcaatt
     1021 attgccttta cagaatctag tccttcgaaa acaatcgcag cttcctggat aatctcaggc
     1081 acgaatttca acgtcgaaga atcaaatgct aaataaaggc taccttccga cgttcggctc
     1141 tgttcacgga aacgttttgc ccgccaatca aatacttgca gaccacccac agaaaaatca
     1201 gataccatca gctgcggttt cttacgcccg ttccattcat tgatcgaaag ctttcctacc
     1261 acatctacct gattattcag cagttcttct tcttgagtac caaaaccaaa agcgaccgca
     1321 tcaagctgac tcgatgcatc ggaaaggaca aatttcagat gctgttggtt tgctccgatt
     1381 cttttgatat tttctgcttg gacttgtcga aataaaaact gaggcaaagg attatccgta
     1441 ccaaatggcg ccaatagttt cagctcatca attcgctcta ctgttacttc atttggcagc
     1501 aatacttcat caattctcaa ctcaggccct ctcatcaagt caatttgatg atcgactata
     1561 tattggttca tcttctcctg tagtatagtg acattttcac ttggcatcgt taaaccgact
     1621 gctgcatgat gtcctccgaa gtaagtaaat aaatctcgca tctggtcaag catttcaaaa
     1681 agattcaagg cttcaatact acgtccagat cctttagctg atccatcttc ttttaaagta
     1741 aggacaagcg ttggtttacc cgtttcattc attattttcc cagcaacaat gcccaaaacg
     1801 ccttcatgcc atcctcggtt taccaataga tggatatgat tctccttatt gaccatcgct
     1861 aatgcttctt gtgttatctg ctctacgatt gcttttcttt cttcattgat ctcattcaat
     1921 ttttttgctt gaacggatgc ttcctcttca tcaaacgtcg caagtaacga gacggcagga
     1981 cgtggatcgc ccatccgacc gattgcattt agtctgggag caatcgaaaa accgatcgtt
     2041 gtttcgtctg cgtccctcat ttttacgccg ctttcttcaa atagcgcctg aagtcctatc
     2101 cgttctgaat gatgaatggc atcgattcct aatgaaacaa ggattcgatt ctcatcagtc
     2161 aatgaaacta aatcggcgat cgttcctatc gctactaaat ccaaaaattc tgcaggcggt
     2221 tcttccaata atgcagaagc tactttgaat gctactccga ctcctgccaa atctggaaat
     2281 ggatagtgcc ccgctggatg acgagggtga acaatcgcat aagcgtctgg taaattctca
     2341 ggtagttcgt gatgatctgt aatgatcacg tctacaccag cttgctgagc gtatgctacc
     2401 gcatcatttc ctgctacccc gttatcaaca gtgatgatca actggattcc ttcttcgatc
     2461 ttttcttggt ataccgattg gtttggtcca tacccatgct caaatcgatt tggcaggaat
     2521 acttctactt ctgcaccaag taattccagt gtttctttca ttaccgttgc actggtgatc
     2581 ccatctgcgt cataatcacc gtaaacgagg attttttctc cactgattac agcttcttgg
     2641 attcggctga ttgctttatc catatcgtgt atcagatacg gatcatgtaa ttcttgttcc
     2701 tttggatgta aaaatcggtt gatatccttt tcctcatggt aacctctttg ccacagtaac
     2761 ttaccgacaa atggagataa tttcagttca cggatcattt ggagaaagga ttcagaaggc
     2821 tctgtctcgc ttaacttcca atcaaaattt gcttgtctca ctttctcact cctaacttag
     2881 ttattatagc aaaggaaatc agcaaaaaaa agcagaaaga aagaataaaa acagatcaga
     2941 tccgctagac agatgttcag caggacctga tcttttgtac gaccatttat gaaatatccc
     3001 tattagtcaa aataattgaa gctttgtttg ggcgattgag ttttttgttc ttccggcgct
     3061 ttcaattgac tgatttgtgc ggttttcgct tgtaactccg cctgataagc agcttccaac
     3121 tccgcacgtt cgtttctaaa ctcgcgttgc tgttcttctt ttgcttcttc aattttcttt
     3181 tgaagttctg tttgatattc tgacatttcc tgagtcagct gtttgatttg ctttttttgc
     3241 tgccatagtg ttgccgaagc agtcaaaaat acaatcagtg caccaatcag cgcagaaccg
     3301 atgatcacta agatcaatgg cgcagaaaag gaagtgaagc caaaacttac aggaacattt
     3361 ttattgttca atacagcaaa aagaacaata atcaaaacta aaacaaatcc aagaatcaca
     3421 ttgctttgct ttttcaattc cttcacctac tttttgttaa aaattccgct ggctaaataa
     3481 tcaccaatcg ttggaaacag cgtataaaat tttgatgcaa cttctaaaat aaacggttga
     3541 ttgatttccc gttttggatg tctcatattg cggacgatca tttttgccaa cttggcaggt
     3601 tctaaaacga tttgtccgac tttttccaaa tagcttccgc ttggatcagc cttatcgaag
     3661 aaatttgtct cgattggacc tggattcact gtcgtaacag ctacgccaag tggtttcaat
     3721 tctaatcgca aagcgttgga gaagccaagg actgcaaatt tcgtggcaga ataaaccgtt
     3781 gattttgcag ttgccatttt tccagccata gaggcgatat taatgatatg accttgtctt
     3841 ctttctgcca tatcgatcgc aaatttttgt gtcaaaacca tcattcctaa gatgttgact
     3901 tcaaacatat cgtatgcttt tccaaggtcg aatgtaagga agtcttcgaa aatcccaaaa
     3961 cccgcattgt ttaccagaat atcgacgcgc ccgacttctt ccatcacctt ttcatataat
     4021 tcgtccacgc tttcaggatt gctgacgtct accttgaaag cataagcctc tttgccgctc
     4081 aagcgtctgc actcgtctct gacaccttct acaaatgcca ttcttcgtgc gcaacttata
     4141 acgattgctc cttgctttgc tgcttcataa cagatttgtg cgcccagacc gcctgaacta
     4201 cctgttacta aaacaacttt atcttctaaa ttcatgcctc ttcctcctcg cgaaatggaa
     4261 tctcaatgct atcaaaatct ttcataatcg tcgtgttttc aaacacttct tgtgcttctt
     4321 tttcaagctc taaagctgct tttcctaaat atctcgcact gatatgagta agtagcagcc
     4381 gttttgcctg cgcttctttt gcaacttcag cagcttgatg agtcgtggaa tggaaatagt
     4441 tgtgcgccat tcttgcttca tctttattaa atgtgctttc atgtaccaga atatcgctat
     4501 tttccgcaag cacaacggaa ttatgtgttt ttctcgtatc gcctaaaata gtcacgattc
     4561 gtcctttttt atctggacct acgaaatctt ttccattgat cgtccgccca tcttctagtt
     4621 ggatcgtttc accttgcttc agtttgccgt acagtggtcc agctggaata cctaattctt
     4681 ttagacgatc cacctgcaat tctcctttat gatcatgttc aaccacacga taaccaaaac
     4741 tgtcgatccc atgatttagt ttttttgtat aaacagaaaa ttgctgatca gtaaaaatcg
     4801 gggctgtttc atttagctca atgaatttca gcggataact taaacgcgtt tgcgaaatac
     4861 ctaaagagac ttttatgtat tcctctatcc cttttggccc gtaaatctct aatggcgtat
     4921 ccccaccttg aaatgaccgg ctgctgatga gcccgggaag tccaaaaata tgatcgccgt
     4981 gtaaatgtgt aatgaaaatt ttaccgattt ttctcggacg aatagtcgtt cgcaaaatct
     5041 gcatttgcgt gccttcgcca cagtcgaaca accaaacttc attgcgctcg tctaaaagtt
     5101 tcaatgctat acttgtcaca tttctatgtt ttgcaggaac acctgcacct gttcctaaaa
     5161 attgtaattc cataatctac ctgactttct ttaagttcct ctttaatttt agggtaaaaa
     5221 gaagggataa tcaaccgtaa gtcgagaatg ggttcaaatt aaaaataacg ttctaaagcg
     5281 atagcgtttt ctttgaaaat tactataaaa acagcagcga acccgtttga gatcctctgc
     5341 tgttttttat taatttgaag atccagaact ttcattgtcg ttggaagttt gcggatgttt
     5401 gtccttccaa gtcttttgtg cagcttcttt cgcatgattt tcagcttctt tcgcatcatg
     5461 cacatctttt tcgacatctt cataatccaa gcgagtaatt tctccgccta attccatcat
     5521 taccaattga ttcagtgggc gattgtcctc ttcatcagca atcaagatca gtcctgtagc
     5581 tccttcaccg attttatttc ctacaaagtc gaacacggtc tgggcatctt ttatttcttt
     5641 ggcatcttgt gtcgcaccga tcattcctcc ggcaaaccat cctaataaaa tccctagcgg
     5701 accacctaat acaccaacca acattccgat taatccaccc gtagaagatt tgttattacc
     5761 ggtaaagtcg ataaaatctt ctattttgaa ttgatgtgtc ccattaggat catggctgac
     5821 aactgccatc tgttctcctt taatcgaacg aacagcatgt aactttttga tttctgaaaa
     5881 tgcctgatat gcctggcttt ctacttgaaa atttaaaatg attactcgtt tgctcattaa
     5941 aaacgcccct ttcatacctt cttgttattt attttacctt taattgaaaa ataagaaaag
     6001 aaaagcactc tttttttcca ataaaatagg gctgtgacaa aagtcgtatc acagctcctt
     6061 tttccgaata aacggcgttc ttgaagctga tcacttctgt cacaacctcc cttaaatctg
     6121 aatctatatt ttttggtatt tgtttttatt tgcttgccta ttcgacaaat tcaaattcaa
     6181 actcaccgat acgtacaagg tcgccatctt tcgcaccgcg tgcccgaaga gcttcatcca
     6241 cacccatacc acgtaattga cgagcaaaac gcatcactgt ttcatcatga tcgaaattcg
     6301 tcatttggaa taatttttcg atcttctcac cagataagat ccacgttgca tctgaatcac
     6361 gatcgatttg gaactcagga ccttctggat tgaatccata gtgtacagtt tcttcttcca
     6421 gttcttcttc gtacaatggg aattctggcg ttacttctaa caaatctgct gtagcattca
     6481 ataacgcgtc caatccttgt cgagtcactc cagaaattgg aaatactggg atgtcatcag
     6541 caaattcatc ttctttttcc ttgttcagct gttctttgaa tttcactaga ttttccggtg
     6601 catccggcat atccattttg tttgccacga tgatttgcgg acgctctagc aagcgtaagt
     6661 tataagtaga taattctttg ttgatggcaa gatagtcttc ataaggatct cgtccttcca
     6721 tcccactcat atcgatcaca tgtaaaatta ctcgtgttct ttcgatatga cgaaggaatt
     6781 gtgtacccag cccaacacct tgcgaagctc cttcaatcaa gcctggcaag tctgctgcgg
     6841 caaaacttct gccatcactt gtagtaacca ttcctagatt aggtacgagc gtcgtgaaat
     6901 gataagcgcc gatttttgga cgagcagatg aaataacgga tagtaaagtc gatttcccga
     6961 cagatggaaa acctacaagt ccaacgtctg ccaatacttt caattctagt tcgatttttc
     7021 gctcttgtcc tggttcaccg ttttcagcaa tttccggtgc aggattccga ggagaggcaa
     7081 aacggatatt ccctcgtcca ccgcgtccac cttttgccac aactagtgtt tgaccatttt
     7141 caattaaatc tcctaaaaga gcaccagtct ccgcatctcg gacagtggtt ccttgtggta
     7201 ctttaacata ggtatcttct gaaccacgac cgtgcatccc tttactcatc ccattttcac
     7261 ctggctgcgc tttgaaatgg cgattgaacc gaaaatccat caatgtacgt aacccttcat
     7321 cgacgatcaa gatgacatcg ccgccacgac caccgtctcc gccagccggt ccgccgtcag
     7381 gaacgtattt ttcgcgacga aatgcaacca ttccgtctcc gcccttgcca gctttcacat
     7441 cgatcgttac ttgatctaaa aacatggaca taattaagtc ctcctattat tcatttattt
     7501 atttgactgc ctgaatttcc acaataaaca aaagtcttac ttattttatc ggtgaaatct
     7561 gattttgtct atcatttcag caaaaagatt gtttaccttc ttcttttttt atcatttggt
     7621 aaggtttcca accttttttc tgctaccaag tataattttt tgacaaaata ttctttgcgt
     7681 agcagaataa cagtgtatta attgtattaa ttgaggatgc cgtttcacag cacccctaat
     7741 aaaggaggaa cttttgatat gacagtaaaa gtaggtatta acggatttgg gcgtattggc
     7801 cgtctggctt tccgccgcat caaagaagtc tcagatgata ttgaagtagt agcaatcaac
     7861 gacttaacaa gtccaactat gttagcacaa ttattgcaat ttgactctac tcatggtact
     7921 tatcctggca cagttactgc aacagaagac tcaatcgtag tggatggcga agcaactcgt
     7981 gtttatgcag aacccgacgc aagcaaaatc ccttgggcaa aagaaaacgg tgtagatatc
     8041 gtactagaat gtactggttt ttatacatct gaagaaaaag caaaagcgca tttagatgct
     8101 ggtgtcaaac gagtagttat ttctgcccca gctggtgcta tgaaaaccat cgtctataac
     8161 gtcaatgatg atacattaga tgaaaacgac aaaatcatct cagcaggttc atgtacaaca
     8221 aactgtttag cacctatggc ttatttctta aataatgaat ttgggatcga agttggtaca
     8281 atgacaacag ttcatgccta cacttccaca caaatgctct tagatggacc tgtaaaaggc
     8341 ggtaatttgc gtgctgctcg ttcagctgct gacaatacta tcccacattc tactggtgct
     8401 gcgaaagcaa tcggcttagt tatccctgag ctacaaggaa aattacaagg acacgcacaa
     8461 cgtgttccag tcgttgatgg ttcattaact gaattggttt cagtattgaa aacaaaagta
     8521 actgctgatc aagtaaacga agcaatcaaa aaacacacag ttgacaatcc ttcattcggg
     8581 tatgatgacc gtcaaatcgt ttctagcgat gtcattggaa caactcaagg ttcgatcttt
     8641 gacccaacgc aaacagaagt aacaactgct ggtgatttcc aacttgttaa aactgttgca
     8701 tggtatgaca atgagtatgg atttacttgc caaatgattc gcttactaga aaaattcgct
     8761 aacttgtaat ttgatagatc aatcgataaa agaagccttt gactggatcc agtcaaaggc
     8821 ttctttcttt tcatccccta cttttaaact cgttcaatga acatattggc ttaaaaattg
     8881 ttggactact tgctgatatt tttcaggatc tgtcttgtat gcttccgcat gctcggctcc
     8941 ttttacaaca tacttttctt ttggtccctt cgttgcacga tagaccttat ctaacatttc
     9001 aaatggtaca aatgtatctt cgtctccatg tataaagagc attgggcgct ggtttttctt
     9061 caattgttcc actgcatttg cttcaccgaa aaagtagcca gcacggactt ttgtcactag
     9121 actcgttact tgcataagcg gaaatgctgg aagactaaac atatcgtcta gctgataagc
     9181 aagctcatca tgaacggaag aataaccgca atcttcaatg attgctttca cattatctgg
     9241 tagtttttct ccgctagtca tcataactgt tgctgctccc atgctgacac cgtatagaac
     9301 gatttcttgc gattttccat tgttttctag cactttgttt atccactgta catagtcttt
     9361 acgctctggc catccaaatc caatgtaatc cccttcgctt tttccatgtc ctcttgcatc
     9421 aggaacaagg acattgtaac ccatatcatg aaacatcttt gcgtaattgg tcatcgtttc
     9481 agcgtttccc atatagccat gagccacaag agctgttttt tcacttttcg tttcagcagg
     9541 aagataaatc gctgataatt tcagaccgtc ttttgaagtg atggtccaat cgcttcggtt
     9601 ttcttttgaa ctaaaccatt tatgtgcatc cgtttctttg gatgtctgtg ttgtaccagg
     9661 tgtatcttcg tctaaaaagt ctttctcaga aggcacaaca gcataattat aaaaataatt
     9721 ccctgcaaac actaacgcaa caatcagagc taaagcgacg cctaaaagga ttttcatcca
     9781 aagtttcata aatcatccat ctcatttctc ttttttcata taaccttgaa ttagtataat
     9841 caaagaatct ctactatgca atgttcattt ttataacccg cgaattgtaa aattaagtta
     9901 gaaaaataaa aaggcattta tggtacactc aaattgtatt tccgacgaaa gaaaaca
//
LOCUS       DAYYHX010000100         9897 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000100 DAYYHX010000000
VERSION     DAYYHX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.101"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..3437
                     /locus_tag="ACHA6Y_001795"
     CDS             159..3437
                     /locus_tag="ACHA6Y_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM8987883.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3978..5885)
                     /locus_tag="ACHA6Y_001796"
     CDS             complement(3978..5885)
                     /locus_tag="ACHA6Y_001796"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM8987884.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6015..6890
                     /locus_tag="ACHA6Y_001797"
     CDS             6015..6890
                     /locus_tag="ACHA6Y_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM8987885.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6970..7944
                     /locus_tag="ACHA6Y_001798"
     CDS             6970..7944
                     /locus_tag="ACHA6Y_001798"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8987886.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8124..9269
                     /locus_tag="ACHA6Y_001799"
     CDS             8124..9269
                     /locus_tag="ACHA6Y_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8987887.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9406..9750
                     /locus_tag="ACHA6Y_001800"
     CDS             9406..9750
                     /locus_tag="ACHA6Y_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM8987888.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3221 a         1814 c         2070 g         2792 t
ORIGIN      
        1 tttttacgtt aataaatctc tttattatca acaacttaag ttatattttt tctttttata
       61 aaatacactt attataattt tgttttttat tgacagtaaa aataaattag gtaatacttt
      121 aagtaaacgc tttctaattt gttacgggag gaatacactt gttaaaaaaa cgtataaaat
      181 acagtgcgct aatgatgaat ctccttatgc tcagtacccc ggttttaatt tctgtcgttg
      241 aagttgctgc taacgaacaa cagtctgtga acgaagaaaa tgcacacgtg aacattgaag
      301 ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga tatggatacg gaagaaggaa
      361 atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa aaagcaatct gaagaagcaa
      421 atgaagagtg gaaaggggtt gttttcggag aaagcacaag tgctagtagg tatgccattg
      481 aaccattaga agacggcgtg cggctctctt ccacgaataa cggcggaaaa ttccagtcaa
      541 gcggatcaga tggaatgacc tattattatc aagcaatccc caaagacatc aactttagga
      601 tgcgggcgac gatcgaaata gaatcttgga cctatacgaa tgcccaagaa ggatttgctt
      661 taatggtaag agacgcagta ccaaaaaatc atttgtttgg gcaggcattt tattcaaatt
      721 catttgccat actaggcagt cggatcgaat atgtctggga ctcggataga caagaagtgg
      781 tcaacacgag cggcagcaaa tatacgatgc gacttggatt ggggacacgg gcaatcacag
      841 gaatatcatc tgaagatccg caagctgcgc cagctcctgg gagtgtctca gtggaaacaa
      901 cacctttaga gacttcagca gggttcttgg aaaaagaaac aggttcttat aatatattcg
      961 gaaacggtca tgggaatctg tttccggcca cgaactcgat tactaaactg gacgtagaga
     1021 tgaaaaaaac gaatactggt ttagaggcat attattacga tcctgaaaca ggagaagaaa
     1081 tggcttctga catcatgtat gactgggaaa aattatacgc ttcagatgaa tcggttattt
     1141 atgcaggatt tgctgcagca aggaatatga cgatcaaggt agaaggaatc gaagttgctt
     1201 actcagatcc tgcgacagat cctcctgggc aagaaagacc tactcgcttg attgatccga
     1261 tttacactgt gacctctagt aatcattcag gaaaccaaga tcatactatg tcctttagag
     1321 caaatgctga cggattattg acaattaaaa ataaagcaac aggagaggtg cttaagaacg
     1381 caaaaaactt ggctatcact gcgaatcggg aatttgatta tcagacaaaa ctgatggaag
     1441 gaacgaacga atttactttc agttttacgc cagataaaaa ctatccgcca gaagaatacg
     1501 tagagatgac tagttatgaa acaaaagaga ttcttcatac agtggactac cgtaaaccta
     1561 aatattcgac agtttatgta acaccagaag ggtcagatgc aggaaacgga agcagacaaa
     1621 atccattatc tctgacacaa gcattgagat acgcatatgc tgggcagaca atcgtcatgg
     1681 cgcctggaac ttattccttc gaaagaggat tgacgattcc aaaaggcgtt aacgggacaa
     1741 gagaaaaccc gattcagttg attgcagaga aaaatccaga aaacaagcgt cctgtacttg
     1801 attttaaagg aactggaaat gggatgacac tgttcagtca ctactggggt ttacgagggt
     1861 ttgacattac aaatagtgca gctatgcaaa aaggattaca gatttctgga aatcataatt
     1921 tgatcgaaga gattcatgct tatcggaatg gaaatacagg aatccaaatc agcggtagct
     1981 caaatgatcc tttcgaagca tggcccgcgc ataacttagt caagaactgt acatcttttg
     2041 aaaatgccga tccaggattt gaagatgccg atggatttgc ggccaaactg acaataggag
     2101 aaggaaatgt atttgacggt tgtatcgcgt atcataatgc agatgacggt tgggaccttt
     2161 ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat caaaaactca gttgcttatc
     2221 gaaacggctg ggtaccagga attgagggag aaggtaacgg taatggattc aaaatgggcg
     2281 gttcttcttt aacgggccct catgaattga tcaatagttt gtctttcgaa aatcttgcga
     2341 aaggaatcga ttccaatagc ggaacagata ttatcgtcaa tagcagtaca agttttaata
     2401 acggttcata taatgtggct ctttatacct cttcagcagg aaacacagac taccatgctt
     2461 ctggtatctt atctttccga acagagaatc ttgagatgag agagcagatc cgcccattga
     2521 atcaaaacga agatcaaatc taccactcgt tgaactacta ttggaatgaa cgagagaaac
     2581 aatcagaaaa tactcttgga cataccatat caaaagattg gatcgagtct ctaaatacat
     2641 gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa tggaagtatc catgttcatg
     2701 gactgatgca gattaaaccg gggaatcgtc aaacagaaaa ggaaagagtg gatggtcttc
     2761 cattatctgt aggtgctatt tttgacggag aaacctcacc gtcttcggaa tatccagagt
     2821 atgcaatcgt acaagaacca aatcagacaa aaccaaatga acaaaataat ccgattatta
     2881 tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa tatcattgaa ctgaagcctt
     2941 ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac gcctgtcttt gaagatatgc
     3001 gttatttccc agaaaagatc caaatcgttg aaacacagtc aagtgctcaa ttttctaatc
     3061 ataccgctat tttttcagca ccttcaaaag gagaatatac gctagaagtc acttatcgtt
     3121 cggaaatatt tgatggggaa aaatggtctg ctcatacaga atctgttacc cagcaaaaag
     3181 cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa tgacaaacca gaaagagaaa
     3241 atccaggaaa tgaatcacca aatggaaaac caaatggaaa accttctcat ccggatagga
     3301 cagcaaaaga aagtgaaaaa aatggcactc aaggactccc aaaaacggga gaatttaata
     3361 acccgttgct agcattagct ggaggaattt tgttaatcgg tgtagttgtt tatgtcatga
     3421 aacagcgaaa aaaataagtg gaatttctcc ttttatgatg taaaacaatt gactgaggaa
     3481 agtaaaaata gaaggaaatg agaccaatag atgtctcact tccttctatc tttttataga
     3541 tgaggttctc cttgctgctt ttgattggct gttcttatta cttgatgcag gacagctttt
     3601 tcacaatctc tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaacaaac
     3661 ggcaaaatat gaggaactat ttttccgttt ttttctctaa tactcaggtc aggacgctga
     3721 tttcacaacc tctcgataaa cggtgttcaa aagccagttt ctcggtagta agtcaaattt
     3781 atgcaaaaat gtggaaagcc attttcacaa aatttttctt actacttgaa actaagcagc
     3841 tttttcacaa cctctaatac atggtattcc agaagtaact tcttcgaaaa ataagccgaa
     3901 atgtcacaaa aatttgagaa gcgattttcc caaattcctt cttatttctt gaagctgacc
     3961 acttctggca caacctctta ccagattcgc actctttttt ctggcgctag atacatagcg
     4021 tcttgttccg tgataccaaa tgtttcatag aattcgtcaa ggttttttgg ttgcacgttt
     4081 gctcggagtt tagcaggggc atggacatcg atttgcagta aaagttgttg gtattctttt
     4141 tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga attcctcgat agaaagatct
     4201 gattccgttt ttgctgcttc caatgcacag cttagaccgc ctgcatcagc aatattttcc
     4261 gaaacggtta attttccgtt tacttttccg tcagcaaaag gcagaccatc gaattcttcg
     4321 atcattgctt gtgctttgtt ttgaaattgt gtaagatctt cttctgtcca ccagttgttc
     4381 aagttgccat attcgtcaaa taaagcgccg ttattatcaa atgcatggga aatctcatga
     4441 gcgataacag ctccgattcc accataatta gcagagcttg attgttctag actgtaaaat
     4501 ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc gaaatggatg ataataagca
     4561 ttgaccgtgt cagcactcat ttcccattct gaacgatcca ctggtttgtt ccatttgctg
     4621 aaacgatctt ttaatgtgag tttgctgaaa acaagagtat ttgaaagcaa agtaccaccc
     4681 tgatctgcag gtactgtttt gaactggtcg aataatgctg ggatcttatc ggggtagcct
     4741 acttggatac ctaagttatt taattttatg acggcttttt cttttgttgc atccccaagc
     4801 cagtcatttt cttctagacg ttttttataa acggcgatca tttttttcac catttgttct
     4861 acgtcttttt tagcagcttc gccaaaatat ttttttccgt agtaatcgcc aactacttga
     4921 tcgaattgtc cagaggcaag gtaatacgct gcttttttct gaggcattgc ttcgtctgtt
     4981 ccagacaata cgcgagaata gattccgctg acttggcgga attcttctga tagataacca
     5041 ctaagagaac gtactgtttt aatgatcatc cagcttttga ataacggaaa atgagcttct
     5101 gtcaggatgt cattcaaatg atcgaagtac actggatcag ttacgatcac tttatctgga
     5161 gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca gtttatctgt gtatttaata
     5221 aatgtatcaa aagattgcgg attatacatt ttgctgtaat ctgcgttttc ttctgcactt
     5281 tttacatgag gagcaatcaa tttatcaaat tgcactgctt gttccgtgat tgcttctgcc
     5341 tgactttttt ctttgccggc aagctctaat aactgaacag ccatatcaaa gaaaacgcct
     5401 attagttgtg caccgttggg atggtctgat gcataatacg tcttatctgg aaggaataaa
     5461 gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct tcatatctgc atctacatcc
     5521 agattaaatg gcaatggtaa tgtatccaac agccattctg gaaattgctt attcaattcg
     5581 tcgaaagact gcaatgattc aactttttcg attataggtt gaagcggttg agcatgcaac
     5641 tcatttcttc ggtcataatc gtttgccagt tgataatatg caaggaaatg tttcattttt
     5701 tctgaagaca ctttttctgg atgggcaagc agatcgtctg tttctcccat caaaagttgg
     5761 tcgatcccgt caactaaatc ttggaaaccg cctgtagctg gtttatctgc agggattttt
     5821 gctgtttttt gccattcttc attgactgct tcatagaaat cttgtttcaa tcgttctttg
     5881 ttcattcatc gatcgctcct tttttgtttc gtgtgcccat tatcttacaa aatgaaggaa
     5941 ttaacaaata gaacataaaa aattgaaaat tcgaacaatc aaactgattt gtttttttca
     6001 atccttcact tataatgaaa aaaaggaggc gatcccaact gaatcaagta gtggataaac
     6061 ctttatattt caaagtagat aaaaaaatta aaaaattagc cagcactcag cagctgcagt
     6121 caagaaaaag taaattgctt tttttagctc tggtttttga agatcaaagt tatgtgatta
     6181 tcgatcaatc aggacatccg gtagaatatt ctccagcaaa atatacttat caagagggtc
     6241 tttcttgtaa aaaatggaag ctgatcaatg aagagccaat cgagttatct agatggatca
     6301 acagaaaaga agatattccc gttttgatcg aagaaaaaag gtcaggaaag gaactcgcaa
     6361 attgctgggt gggattgcca gaagaacgtt ttttgaggta taagaaatgg gctaccccct
     6421 caggctatct ttgtggtact tacgcggcag cagttctcct tgcctattat caagactatc
     6481 gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa tacggctgat tcgttggttt
     6541 tgacgaaggc attgcgtagc cagatccaac cactaggact tcctacgatt ccttttcagg
     6601 ttagtacagg gatttctagt tttttaaaga agaatggcaa tcatgagcgt gcacgagcaa
     6661 ctcttctagg ttcatggcag cgagctacaa aacgtatacg tgaaggcaaa cctgtgatga
     6721 ttggtatttt gaaagtactt ggcagtacat atggcaatca ttgggtgaca gcgtacgctt
     6781 attttgaaac tgaaacagga gaacgttatt acaaagtaca cgacaactgg ggcgattatc
     6841 ataaagtcat tcctgccagt tggagtaacg gaacagtttc acttccttaa gaatttgctg
     6901 aaattaacag aaaatcttgg aaaaatatgt taaactatcg aagaatagag aagttttggg
     6961 gaggactaca tgagtaataa gtatcaggat gacacgttga agatttttag tttgaatggc
     7021 aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa cagaattagg taaaagcgtc
     7081 gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg aagaaagtat tcgaggggat
     7141 catgtgtata tcgttcaatc aacgaatgca cctgtcaatg attactatat ggaactactg
     7201 atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga tcaatgtcgt attgccttac
     7261 tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg aaccgatcac tgcgaaattg
     7321 atcgctaact tgatcgaaga agcaggggca actcgagtac tgactttaga tctgcacacg
     7381 gttcaagtac aaggattctt tgatattcct gtagataatt tatttacgat gccgttgttt
     7441 gctcattatt accgtcaatt aggtctgacg ggagatgaca tcgtagttgt ttcgccaaaa
     7501 aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt tgaacagcac attagccatc
     7561 gtagatcatg cagatgatgc ttcaactgag ggtggttatg tgatcggtga tgttcaagga
     7621 aagacatgta tcatggtaga tgatatcttg aacacaggag caacgcttgc acgagcagca
     7681 aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct gtgcgtcaca tggattattg
     7741 tccgatcatg cgaaaagtat tttagacaat gcaccaatca aagatatttg tatcactgat
     7801 tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa caatcgttac atgttcagaa
     7861 ttaatgggtg aagcggtcaa acgcatccat gaaaatacac cgatgagtcc gttattccgt
     7921 ctggaagaaa aagaattcga ataaacaaaa aaaagaggga aagaagttgt gacagaagtg
     7981 agcagcttca aggagctgcg acaagacttt tgtcacagct ccttttttct atgcccatga
     8041 aacgtcctct agacaaaaat gagctcttct ttggtttgtt cgctatttta tgctagaatg
     8101 tgcctagcaa taatttggaa gggatgaatg aagtgaaaac tatttatctg gatcatgcgg
     8161 caactacccc catgcatcca caagtaattg aagcgatgac ggcaatcatg caagacacgt
     8221 ttggaaatcc ttcaagcata catggatttg gtcgccacgc gcatgaaaaa ttggaaacag
     8281 caagacaaat cattgcagac agtctgcaag caaaacctca cgagatcatc tttaacagtg
     8341 ggggaacaga aggggataat acagctatct tagaaacagc gttctcgcgt caaaaagaag
     8401 gcagacatct aatcactaca gctatcgaac atcctgctgt tcttaatacc atgaaatatt
     8461 tagagacaaa ggggtttgac gttacatatc tgcctgtaga tgaaaaaggg aatctgtcga
     8521 tcgaagaggt caaaaaagcg ttacgagaag atacgatctt ggtctctgtg atgtacggga
     8581 acaatgaaat cggaaatttg atgccaatca aagaaatcgg tgaactttta gcggatcatc
     8641 cagcttattt ccatacagat gcggtacaag cgtacggaaa ccaaacgatc catccagaag
     8701 aattaggaat cgatttatta agtatctctg ctcataaaat caatgggcca aaaggcgtcg
     8761 gtttcttata taaacgagac ggagtaaata tccctgcatt gcttttaggc ggggagcagg
     8821 aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat ctggggtatg gcaaaagcag
     8881 ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa caaatataaa ggattccagc
     8941 aattgattct tgatacatta gaagcaaacg aaatccctta tcaagtaaat ggtgatttaa
     9001 cgaataaact ccctcatgtg ttgaatcttc gcttaattgg cgaagcaaat gatttgctac
     9061 tgatgaagct tgatcttaat ggggtcgcta tttctactgg ttcagcttgt acagcaggaa
     9121 atatcgagcc ttcacacgta ttagaagcaa tgtatggaga agatacaccg atactaaaag
     9181 aatccatccg tatcagcttt ggtttgggaa ataccgaaga cgacatccag tatttccttg
     9241 atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc attcttatat gaacagatgt
     9301 ctgagagaac gccttcattt tttagggaac ttaactgaaa aacctagtaa aaatcaggat
     9361 aaacttttat aataaagaga aactaatttt atcgaggtga aaatcatggc atttgaaaca
     9421 acagcaacgg tactgggcgc accaattagc tatcgtttgc atcctgatgc aaaaagatat
     9481 acattgcgcg ataatggatt tacagaaaca aacggaggca attatcaatt gatccgacca
     9541 ttagatgcca cgccgcaaag caaagaagga ttcaaattaa aaatcactgt atcaaaagat
     9601 attcaaacat tgaaaatgtc gatcactaca gcaaatggat tgaaagcagt caatattttc
     9661 aaagatccaa aacagaaaat gagccaagat aaattttatt ttctgatgga tgggatgatt
     9721 agccgtgggt tattcgaaaa agtcgaatga tctgtggata taaaaagaca actagttttg
     9781 aaacggccta ttgttagatg actaactttg taaggacgac gcaaattagt tgtctttttt
     9841 atttttaaat ttaacacatg ttgttatttt taatattttg ctgatttatt gtttcta
//
LOCUS       DAYYHX010000101         9704 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000101 DAYYHX010000000
VERSION     DAYYHX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.102"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..646
                     /locus_tag="ACHA6Y_001801"
     CDS             23..646
                     /locus_tag="ACHA6Y_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987889.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /gene="trpX"
                     /locus_tag="ACHA6Y_001802"
     CDS             1096..2145
                     /gene="trpX"
                     /locus_tag="ACHA6Y_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8987890.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="ACHA6Y_001803"
     CDS             2142..3023
                     /locus_tag="ACHA6Y_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8987891.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="ACHA6Y_001804"
     CDS             3026..3796
                     /locus_tag="ACHA6Y_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8987892.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="ACHA6Y_001805"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="ACHA6Y_001805"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM8987893.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="ACHA6Y_001806"
     CDS             5107..6270
                     /locus_tag="ACHA6Y_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8987894.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="ACHA6Y_001807"
     CDS             6387..6797
                     /locus_tag="ACHA6Y_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8987895.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="ACHA6Y_001808"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="ACHA6Y_001808"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8987896.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="ACHA6Y_001809"
     CDS             9370..9654
                     /locus_tag="ACHA6Y_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987897.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT         3363 a         1647 c         2087 g         2607 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtc
//
LOCUS       DAYYHX010000102         9664 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000102 DAYYHX010000000
VERSION     DAYYHX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.103"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..632)
                     /locus_tag="ACHA6Y_001810"
     CDS             complement(<1..632)
                     /locus_tag="ACHA6Y_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8987898.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="ACHA6Y_001811"
     CDS             975..1391
                     /locus_tag="ACHA6Y_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8987899.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="ACHA6Y_001812"
     CDS             1388..1858
                     /locus_tag="ACHA6Y_001812"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8987900.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="ACHA6Y_001813"
     CDS             1872..2645
                     /locus_tag="ACHA6Y_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8987901.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="ACHA6Y_001814"
     CDS             2659..3471
                     /locus_tag="ACHA6Y_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8987902.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="ACHA6Y_001815"
     CDS             3525..4490
                     /locus_tag="ACHA6Y_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8987903.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="ACHA6Y_001816"
     CDS             4606..7329
                     /locus_tag="ACHA6Y_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8987904.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="ACHA6Y_001817"
     CDS             7342..7974
                     /locus_tag="ACHA6Y_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8987905.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9664
                     /locus_tag="ACHA6Y_001818"
     CDS             8873..>9664
                     /locus_tag="ACHA6Y_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8987906.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3260 a         1477 c         1884 g         3043 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agctacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaaccgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtat
//
LOCUS       DAYYHX010000103         9503 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000103 DAYYHX010000000
VERSION     DAYYHX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.104"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACHA6Y_001819"
     CDS             99..746
                     /locus_tag="ACHA6Y_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8987907.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACHA6Y_001820"
     CDS             1139..3784
                     /locus_tag="ACHA6Y_001820"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM8987908.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACHA6Y_001821"
     CDS             4094..5407
                     /locus_tag="ACHA6Y_001821"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM8987909.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACHA6Y_001822"
     CDS             5397..6050
                     /locus_tag="ACHA6Y_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8987910.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6796
                     /gene="radC"
                     /locus_tag="ACHA6Y_001823"
                     /pseudo
     CDS             6105..6796
                     /gene="radC"
                     /locus_tag="ACHA6Y_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6946..7146
                     /locus_tag="ACHA6Y_001824"
     CDS             6946..7146
                     /locus_tag="ACHA6Y_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8987911.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7400..9079)
                     /locus_tag="ACHA6Y_001825"
     CDS             complement(7400..9079)
                     /locus_tag="ACHA6Y_001825"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8987912.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9081..9293)
                     /locus_tag="ACHA6Y_001826"
     CDS             complement(9081..9293)
                     /locus_tag="ACHA6Y_001826"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM8987913.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
BASE COUNT         3020 a         1696 c         2031 g         2756 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaacagtgtt
     6541 catcggttcg ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg
     6601 ttattgtgca gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc
     6661 gcagcaggat atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac
     6721 tgtactggac cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat
     6781 gatggaagag aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt
     6841 gtaattttgt ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa
     6901 tgttacaatt taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca
     6961 gtaaaatggt tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac
     7021 gtattcgttc acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa
     7081 gcagtgactt tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa
     7141 gcttaatttt atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag
     7201 cgagtaacaa aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta
     7261 tgaaaaagta aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa
     7321 caaacaaaaa tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc
     7381 tgatttcata acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg
     7441 gatgtctttc tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca
     7501 atttatattc tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc
     7561 gctgagcttc attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa
     7621 tatctggacc agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa
     7681 gaccttcttc tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc
     7741 cgctaccatc gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc
     7801 tagttaacgc taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta
     7861 catcttgtgc tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa
     7921 aatattttgg cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg
     7981 aagtatggat gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat
     8041 tgatcaaatg attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg
     8101 tatcgtctgg ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg
     8161 ccatcgcttc tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat
     8221 tgatttcgtg tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac
     8281 cgttgacgat ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca
     8341 cagcggcatc tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga
     8401 tacgtccttc gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt
     8461 tagtaaatgt tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt
     8521 ccccgatacg agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt
     8581 tgaagtctcc agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat
     8641 ctgggatact atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg
     8701 tatcttcatt gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt
     8761 ttgtgatcaa cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca
     8821 agtagggtat tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt
     8881 tgtgtaagtt ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat
     8941 cgtcttccgg gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc
     9001 catacgtatt tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat
     9061 ttttgatgga aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg
     9121 tactctagat gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct
     9181 tccacacgtt ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct
     9241 tcccgtttag ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg
     9301 tctcctttat ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac
     9361 gaggggacaa gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa
     9421 gagacaagcc tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata
     9481 aaaaatatac aacaattaat tgt
//
LOCUS       DAYYHX010000104         9462 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000104 DAYYHX010000000
VERSION     DAYYHX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.105"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..538
                     /locus_tag="ACHA6Y_001827"
     CDS             11..538
                     /locus_tag="ACHA6Y_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM8987914.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            601..1971
                     /gene="radA"
                     /locus_tag="ACHA6Y_001828"
     CDS             601..1971
                     /gene="radA"
                     /locus_tag="ACHA6Y_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM8987915.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            2005..3162
                     /locus_tag="ACHA6Y_001829"
     CDS             2005..3162
                     /locus_tag="ACHA6Y_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM8987916.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            3332..4792
                     /gene="gltX"
                     /locus_tag="ACHA6Y_001830"
     CDS             3332..4792
                     /gene="gltX"
                     /locus_tag="ACHA6Y_001830"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM8987917.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     misc_binding    4789..5028
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5114..5650
                     /gene="epsC"
                     /locus_tag="ACHA6Y_001831"
     CDS             5114..5650
                     /gene="epsC"
                     /locus_tag="ACHA6Y_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM8987918.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            5647..7059
                     /gene="cysS"
                     /locus_tag="ACHA6Y_001832"
     CDS             5647..7059
                     /gene="cysS"
                     /locus_tag="ACHA6Y_001832"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM8987919.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            7056..7451
                     /locus_tag="ACHA6Y_001833"
     CDS             7056..7451
                     /locus_tag="ACHA6Y_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM8987920.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            7444..8304
                     /gene="rlmB"
                     /locus_tag="ACHA6Y_001834"
     CDS             7444..8304
                     /gene="rlmB"
                     /locus_tag="ACHA6Y_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM8987921.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            8308..8838
                     /locus_tag="ACHA6Y_001835"
     CDS             8308..8838
                     /locus_tag="ACHA6Y_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM8987922.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            8915..>9462
                     /locus_tag="ACHA6Y_001836"
     CDS             8915..>9462
                     /locus_tag="ACHA6Y_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8987923.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKI"
BASE COUNT         3230 a         1724 c         2136 g         2372 t
ORIGIN      
        1 aggaaaaccg gtgagaaaaa gaggatttga agttatttct aaatataaag atcaaaatat
       61 caatctaccg cagcgcgcta cacatcatgc tgctggctat gattttgaag cagcagaaga
      121 ggtcgtgatt ccaagtatct ggaaagcagg ctggaaaaag gggttggaaa cagtcgcaga
      181 tggattgaaa aaagatgtat cagaaaatga aaaacaattg aaaccaaccc tcgtgcctac
      241 tggaatcaaa gcttacatgg gagaagatga atttttgcag cttgctaacc gttcaagcaa
      301 tccgttgaag cggtttttag tgttgacaaa cggagtaggt gtgattgaca gtgattatta
      361 caataatgag gcaaatgaag gccatatcat gttccaattc accaactttg gtttcaagga
      421 tgtagtgatc aaaaaaggcg agcgtatcgg ccaagggatc tttttgccgt ttttaaaagc
      481 agatcaagat caaacgactg ctcaacgtca aggcggattt ggttcttcca gtaaataaaa
      541 cgttaaagat acttaagctt gatttaatgt ctcatgatta ggataaaagg aggcggtaca
      601 atggcaaaaa aatcaaaagt acaatttgtt tgccaaaatt gcgggtatgt gtcgcctaag
      661 ttcttgggac gctgcccaaa ttgcggaaaa tggaatacga tggtcgaaga aatcgaacaa
      721 gatacaacgg accgcagaac tcggacaagt ttgacaggag aaaaagcaaa accaacaaaa
      781 atcgcagacg tcgtccctaa aaaagaacct cggatcaaaa caaaattaga agaactgaac
      841 cgtgtgttag gcggaggtgt ggtgccaggg tctatggtct tgattggtgg cgatccaggt
      901 atcgggaaat ccactttgct gttacaagtt tctcagcaat tagcagccat aggcggaaaa
      961 gtcttatatg tatccggcga agaaagcgca gagcagataa aattacgcgc ggaacgcctc
     1021 ggaagtatca acacagaatt ttatctatac gctgaaacag atatgaacga aatcagtcgt
     1081 gccatcgaac atatctcccc tgattatgtt atcattgatt ccatccaaac gatgacgcag
     1141 ccagacatca caagtgtggc aggaagtgtc agccaagtaa gagaaaccac tgcagaacta
     1201 ctcaaaatcg ctaaaacaaa tgggatagcc atttttatcg tcggtcatgt taccaaagaa
     1261 ggctcgatcg ccggcccaag aatgttagaa catatggtgg atacggtgtt atattttgaa
     1321 ggtgaacaac atcatagttt tcggattttg cgagcagtaa aaaaccgatt tggctcaacg
     1381 aatgagattg ggatttttga aatgcatgag catggattgg aagaagtcgc taatccttca
     1441 cagattttct tagaagaacg cctagacggt gcaactggct ctgctatcgt tgtggcaatg
     1501 gaagggacac gtccaatttt ggtagagatc caagcattag tcacacctac tatgttcgga
     1561 aatgccaaac gaacaacaac aggtcttgat ttcaatcggg tatctctgat catggcagtg
     1621 ctggaaaaaa gagctgggtt gcttcttcag aatcaagatg cttacttaaa agctgcaggt
     1681 ggggtaaaat taaatgaacc agcgattgat ttagctatcg ctgtcagtat tgcttcaagc
     1741 tataaagaaa aaggaacaca gccaacggaa tgtttcatcg gtgaaattgg actgactgga
     1801 gaaatcagac gtgtcagtca tattgaacaa cgagtcaaag aagtccaaaa actcggcttc
     1861 acaaaagtat acctacctaa aaacaactta ggtaactggg aagcgccaaa aggaatcgag
     1921 atagttggag tagcaacttt ggctgaaacg ttgaagcgtg tgttccgcta actaataaat
     1981 gattgtcgaa acggaggaaa aacgatgcaa aaacgtgtca ttactttgct gatgatcatt
     2041 gtcggcgcaa gcttgggtat ttcattttta ccctcagctt ggcaggcaat cggtcaacag
     2101 tcaaacggat ggctgaataa tacatttacc aacagcctgt taggcgcact tattttcttt
     2161 ttattatctt tgctgttggc caaacatatc tcagctatta tcaaacaagt agaacaaaaa
     2221 ctaagtgagg tcagcttgac ttatcttctc ttcggtgcga tcggtgcgat catcggattg
     2281 actttaggtg ttgtgatctc gacccctctt tacaatatgg aaattccatt cgtcaatagt
     2341 gtattgccga ttttactgat gatcatctta ggctatctgg gattgagaat gggaacgaca
     2401 cgaatcgaag aatggaagaa agtttttggt tcacgaagca aaaaaatcga gcaagaaaca
     2461 gctagtcaag ttgagagtca actgttagaa cgaaaatcag gagaaaattt ccataaatat
     2521 aaaatcttgg atacaagcgt gatcattgac ggtcggattt atgatattac aaagactgga
     2581 ttcttagaag gaaccttgat gatccctaat tttgttcttt acgaactaca atacatcgcg
     2641 gactcagggg atagtttgaa acgtgtgcgt ggtcgtcgcg gattagatat cctaaatgcc
     2701 ttgcaaaaag aagagggtat atctgtcgaa atgtacgatg gagactttga agatatcagt
     2761 gaagtagaca gcaaattgat caaattggct aagcttttag atggtgtcat tgtaaccaat
     2821 gactataact tgaataaagt ctctgaattc caaaatgtac ctgttcttaa tatcaatgcg
     2881 ttggcaaatg ccgtaaaacc agtcgtcatc cctggtgaaa cgatgaatgt tctagtagta
     2941 aaagccggga cagaacgcca acaaggagta gcctaccttg atgacggcac gatggttgtc
     3001 gtagaagatg gacagcatta catgaatgaa cgtatcgaag tcgttgtaac aagtgccctt
     3061 caaacagctg ctggacgcat gatctttgct aaacctgcac atgcaggacg aggaatcgac
     3121 aataaaaaag agacagagaa aaagactaaa caatcaaaat aaaaaagaaa ttcagccaac
     3181 tggtaaggaa attcaaccag ttggctttac tatttacctt agaaaaggta aaatttgtgc
     3241 tgagtggctt taaataaatg atatttgaga agccgtaact gacggaattt ttgctgttag
     3301 tcaaagtcta gtttgaaaga agaggaagaa aatgacgaaa gtacgcgtac gctatgcacc
     3361 aagtccaact gggcacctgc atattggaaa tgcaagaact gcattattca attatttgtt
     3421 tgcccgccac aatgatggag actttattat tcgtattgag gatactgacc aaaaacgtaa
     3481 tatcgaagat ggcgaaaaaa gccagttgga aaaccttgct tggttgggaa tgaattggga
     3541 tgaatctcct gaaaatcctg gagaatacgg cccttaccgt caatctgaac gtaaagaaat
     3601 ctatcaaccg ctgattgatc agctgcttgc tagcaatcga gcatacaaat gctattgcac
     3661 agaagaagaa ttagaagcag aacgggaagc acaacgcgca agaggagaaa tgcctcatta
     3721 cgcaggaaca tgtgcgaact tgacacctga agaacaagca gaaaaagaag cacaaggtct
     3781 agaatctgtt gtacgtttcc gtgtgcctcg caatacagaa tacgcattta cagatatggt
     3841 gaaaggcgct atttcgttcg aatccgataa tatcggtggc gactttgtca tacaaaaacg
     3901 cgatggtatg ccaacttata attttgctgt tgcggtcgat gatcatttga tgaaaatcac
     3961 tcacgtttta cgtggagatg accatattgc caatacacca aaacaactga tgatctatga
     4021 agcatttggc tggacaccac caaccttcgg acatatgaca ttgatcatca acagtgaaac
     4081 aggtaaaaaa ctaagcaaac gtgacgaatc aatcttgcaa tttatcgaac aatatcgcga
     4141 attaggctat ctaccagatg caatgttcaa ctttattgca ttattgggct ggtcacctgt
     4201 gggcgaagac gaaatcttcg aacaccaaga attgatcaaa atgttcgatc caaaacgcct
     4261 aagtaaatca cccgcagctt tcgatgcgaa aaaactgcaa tgggtaaaca accattacat
     4321 caaagcaatg gatctagatg catttactga catgtgtctg ccttatctaa tcgctgatgg
     4381 acgtgtagca gaaaacccag atgaaaagac aatcgaatgg gtgaagaaga tcgttagcct
     4441 gtatcaacct caaatgagct atgctgcaga aatcgttgat ctgtctacgc tatttttcaa
     4501 tgaacatcct gtattgaatg aagcagcaaa agaagtactt gctggtgaaa cagttcctac
     4561 tgttctccac gcattcaaag cacagctaga acaaatggaa gtagtagatg ctccaactgt
     4621 caaagctgcc atcaaagctg ttcaaaaaga aactggcgta aaaggaaaaa atcttttcat
     4681 gccaatccgt gtagctgtat caggacaaat gcatggtcca gaattgcctg aaacgatcga
     4741 attgcttggt cgtgaaaaat cattagcaca tttaaatcaa gtactgaact aaacacgatg
     4801 aacagaaaaa agtaagcagc tgatcattac aagagagata ccgggtggtg caaggtatca
     4861 atgaatggtt gccgaaatgc ctctgggagt ggtcttggcg aaacacagaa aacaggaaaa
     4921 actgttttat aggaaacaaa gtagccaaga acgtataaca ggcgttagct gtcagagata
     4981 atcattgatt atgaagaaaa gtggaaccac gcggaaagcg tcttttgata aggaaactta
     5041 tttaaaaggc gcttcttttc tttataagaa gatgatttcc tacataaaat tcttacgaaa
     5101 ggagtggaaa gtcatgagtt ggtttacccg agcgattgat gcagcaaaga gaaacgatcc
     5161 ggctgcacgt tcgacagctg agatcgtttt gacataccct ggttttcatg ccttgttttg
     5221 gcatcgtttc tctcattttt tatatcagca ccggctcttt ttattggcga agatcaatgc
     5281 tcaattctgg cgtttcctaa ctggtgttga gatccatcca ggagcaacga ttggaactgg
     5341 cgtgttcatc gaccatggaa tgggaatcgt cattggcgaa accgcagaaa tcgaggacga
     5401 tgtgatttta ttccatggcg taacattagg cggcacagga aaagaaacgg gtaaaagaca
     5461 cccaactgtt aaacaaggag ccatgctctc tgcgaatgcg cagatattag gaccggtaac
     5521 gatcggaaaa aacgccaaaa tcggtgcagg tgccgttgtg ttgaaagatg ttccagacga
     5581 cgctacagcg gtaggagtac cagcaaaagt cgttcgaatc aaaggagaaa aagtgaggag
     5641 atcagaatga taaaaatcta caatacattg accagaaaaa aagaagaatt catccctata
     5701 caaaagggac atgtcggtat gtatgtctgc ggaccgactg tgtataatta tatccatatc
     5761 ggaaatgctc gtagcaccat tgctttcgat acaattcgac gctattttga atatcgaggc
     5821 tataaagtcg atttcgtatc gaatttcacg gatgtagacg ataaaatcat ccgtacagca
     5881 aacgaattag gtattacagc accagaagtt gctgatcgtt tcatccgagc atttgaagaa
     5941 gatacgaaag cgttgaatgt cgagcctgct acactgcatc caagagtcat ggatcatatg
     6001 ccagatattt tagcatttat tcaagtgttg gttgaaaaag gctatgctta tgaatcttca
     6061 ggtgatgtgt attaccgtac ccgtaaattt gaaaattatg ggaaattaag tgaccaatcc
     6121 atcgatgaac tggaagtcgg agcaagtcag cgtacagggg aagaacagca gcaaaaagaa
     6181 gacccattag atttcgcttt atggaaacaa gcaaaacctg gcgagatttc ttgggattca
     6241 ccatggggca aaggacgtcc cggttggcat attgaatgtt ccgttatggc aacaaaacat
     6301 ttaggagaaa cgatcgatat ccatggtggc gggcaagatt tagaatttcc tcatcatgaa
     6361 aatgaaatcg cacaaagtga agcgaaaaca gggaaaaaat tcgctaatta ctggatgcac
     6421 aatggctatg taacgattgg cgaagacgat gaaaaaatga gtaaatctct agggaatttc
     6481 atcactgtac atgaaatgat caaaaacgtt gatccacaag tcttgcgttt cttcatggcg
     6541 accacacaat accgtcgtcc gatccgctac agtgaaacga cgatgaaaga agcagccacc
     6601 aatttacaac gactgaagaa tacgtttgac aatttgaaat tccgccaaga aactgcctca
     6661 gagaatttgg cagatgatgc ggataaaatc gctttgttag aaaatttaga acagcgattc
     6721 atcgaagaaa tggacgatga ttttaatgca gcaaatggga tcacagtggt ctatgagctt
     6781 gctaagtggc tgaacactta tctagaccag ccgaaagttt ctgccgctgt cttggcacaa
     6841 ggagaagctt tattctcgca atggctttcg atcttcggta ttttatttac agcagaagag
     6901 ttattagatg aagagattga acaattgatc gaagaacgaa atcaagcaag aagcaatcgt
     6961 gattttgtcc gaagcgacga aattcgtgat ttgttgaaag aaaaagggat cttattagaa
     7021 gatacaccgc aaggaacccg atggagaaga gaagcatgag agattacaca caattgaacg
     7081 gtttagcctt ggcttatgtt ggagatgcga tttacgaaat ctatgttcgt gaccacctga
     7141 ttgatcaagg agaaacaaaa cccaataagc tgcatcgtgc ggcaacgaat tatgtttctg
     7201 cgaatgcaca agcctttttg attcagcaaa tgctggaaga acatcttttg acagaagaag
     7261 aagaacttta ttatcgcaga ggaagaaatg ctaaaagcca tacgagcgca aaaaatgctg
     7321 atgttacgac gtatcgggtc gcaacagggt tcgaagcgct catgggctat ctgcatttga
     7381 caaaacaaac cgagcggatg gaagaattga tccgctggtg tattcagaaa gttggtgaaa
     7441 aaaatgggta aagaaagaac aaacaaacat caatcgaaac gtggatttga agacaaaaaa
     7501 caaaatagat attcaaataa aaagcaaact ggacgaaatc agtcagtttc tgatcaaaat
     7561 gaagaagcat tagaagataa tttcgtcttt gggcaccatg ccgcagtaga agcgctacaa
     7621 caagggcgag ggaataaact ctttcttcaa gaagattcac gaggagaaaa agtagaagaa
     7681 ctgaaagaaa tcgcacgtga acaagctgtg ccagtcaaat gggttcctaa acaaaaatta
     7741 gaaactttga gcgatcacgg cgtgcatcaa ggagtcgttc ttgcgattac accttatgaa
     7801 tatttatccc ttgaccaact gattgaacga acaaaagaga aaaaagaaga ccccttcttt
     7861 ttgattttgg atagcatcga agatccacac aattttgggt cgattttacg gacagcagac
     7921 gcaagtggcg tcgatggtgt gatcatacca aaacaccgag cagttggaat cacacctatc
     7981 gtaacaaaga cttccactgg ggcagtcgaa catgtccctg ttgcacgcgt gacgaatttg
     8041 gtacaagctg tgcaacagct caaagaaaca ggattttggg tatttggcac agatatgacc
     8101 ggcacaaact tccaaaaatg gaatgccaaa ggatcaattg ctttagtcat tggtaatgaa
     8161 ggcaaaggaa tgagtccagg attgaaaaaa gaaatggatg aattgctgac gatcccgatg
     8221 accgggcacg tccagagctt aaatgccagt gttgctgctg gtttgcttat gtatcaggct
     8281 ttttcccaac gtcaggggga gtagaccatg aaaaaacagc tgttgattgt agacggctac
     8341 aacatgattg gttcgtggcc ggaacttgtc cagcttaaaa agcaggacaa aatggcggat
     8401 gcaagagaag ctcttttaca tcgtttatca aattatgcga aatatgaagg aatggaagtc
     8461 atcgttgtct tcgatgccca actagttccg ggcatccaac aaaattataa gaaataccag
     8521 ttggatgttg tttttacgaa agaagacgaa acggctgaca gttatatcga gagaatcgct
     8581 ggtcagctga atgaccgttt gacacaagtg acagtggcga caagcgattt agcagaacag
     8641 tggatgatct tttcccaagg agcattacga acttctgctt atgaattata taagacgata
     8701 aaaaaaacgg aaaaaacaat agcccaagaa aataaagata tgcattttat tgatttccga
     8761 cgtaattctc catggaatga agagcagtta aaaaaattgt cccaaaaatt ggacgattta
     8821 atgagaaaaa ataaataaca tatcgttcta atcagttcga tgaatcattg atgataagat
     8881 tcctccataa acgaatttat ggaggaatta ttttatgtct gaggagttat tgcgcgctgc
     8941 acgtaatggt gacgaagaag catttgctgc cctgtatcaa aaatattttc cacttttact
     9001 acgtttgaaa aacacctatt atttaagaaa ttatgtttct gaggattggg aacaagaagc
     9061 aaggatcgct ttatatcagg cattgagaac ttacgaaaaa agcaaaggtg ttagctttgg
     9121 aagttattat aaaagcatta taagcaatca aatctattct ctgttgagga agcagaacgc
     9181 gctgaagcgt cgtgatcaaa aagatgaagt ctcaatcgac caaaaaatag agacagaagg
     9241 accagacttt ttagcagaga tcacattgca agaaccttta gcgattcagc atcttttatt
     9301 aaaagaagca atcgaaaatt gcgaaatcca gttttcaaaa atagaagcta aaatttttca
     9361 ttattatatg caaggcgttg attttgtaga catggctgaa tgtttagcaa tggatcacaa
     9421 gcaagtaatg agcgcttacg ggagagtaaa actcaaaata aa
//
LOCUS       DAYYHX010000105         9432 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000105 DAYYHX010000000
VERSION     DAYYHX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.106"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..680
                     /locus_tag="ACHA6Y_001837"
     CDS             <1..680
                     /locus_tag="ACHA6Y_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8987924.1"
                     /translation="YFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHI
                     VQHIGRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRAS
                     FKDYLTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETY
                     TTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRH
                     GKTFLTK"
     gene            800..1000
                     /locus_tag="ACHA6Y_001838"
     CDS             800..1000
                     /locus_tag="ACHA6Y_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8987925.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1076..1273
                     /locus_tag="ACHA6Y_001839"
     CDS             1076..1273
                     /locus_tag="ACHA6Y_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987926.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1570..3117)
                     /locus_tag="ACHA6Y_001840"
     CDS             complement(1570..3117)
                     /locus_tag="ACHA6Y_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFM8987927.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3201..4004)
                     /locus_tag="ACHA6Y_001841"
     CDS             complement(3201..4004)
                     /locus_tag="ACHA6Y_001841"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8987928.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACHA6Y_001842"
     CDS             complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACHA6Y_001842"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFM8987929.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACHA6Y_001843"
     CDS             complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACHA6Y_001843"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFM8987930.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACHA6Y_001844"
     CDS             complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACHA6Y_001844"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFM8987931.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6389..7318)
                     /locus_tag="ACHA6Y_001845"
     CDS             complement(6389..7318)
                     /locus_tag="ACHA6Y_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8987932.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7513..8196
                     /locus_tag="ACHA6Y_001846"
     CDS             7513..8196
                     /locus_tag="ACHA6Y_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFM8987933.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8357..9400
                     /locus_tag="ACHA6Y_001847"
     CDS             8357..9400
                     /locus_tag="ACHA6Y_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8987934.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2893 a         1933 c         1869 g         2737 t
ORIGIN      
        1 gctatttttc tcggttttca ttagcgactc gtgaagccgt tcaatggatt gttattgata
       61 tgtatgcgcc ttatgtttca ttggtgaaaa agcttttccc gaatgctcag ttgatcatcg
      121 accgtttcca catcgtccaa catattggca gaaccttctt gaatcatcga gtgaaagaaa
      181 ccacggcacg gcttaaagga acttcccact ctcaacgagg gttagggaaa aaattgaaac
      241 gctattggaa actcctacaa aaagaagaag caaagttaaa ctacgaaaaa agggtttggc
      301 gtgccagttt taaagattat ttgactgaaa gtgagattgt cgatcgactg ttatctgctt
      361 gtcccaacct gcgccaaggc tatcagttat accaagacat tctttatgcg gtaaagaaaa
      421 gagatcagtc attatttgag gactgtttaa acagagaagt cagcggtctt cctgaaacct
      481 atacaaccac tttacgaacg tttaaaaagt ttctgccaca gatccaaaat gcgcttcact
      541 acagctattc aaatggacca ttggaatgtt taaacaacca catcaaagta ttaaaacgca
      601 atgcgtacgg ctttcgcagc ttttacaatt tcaaactacg gatcatgatt cgtcatggca
      661 aaacattttt aaccaaataa acagaacaga aagagagttt cttctttctg ttctgtccaa
      721 attattctaa gatttctctt taccaacatc atttgacaaa gagcctgtat ttattactgt
      781 cgaggacaat ggcatcggca tgattgaaga ggaacaaaaa aacatttggg atcgctatta
      841 taaagccgat ccgtcaagta aaaatacaaa gataggcgag tcaggtcttg gattatcaat
      901 cgtgaaggaa ctagtgcgcc tgcaccacgg ttcaattgaa gtgaccagcc gaaaaaatga
      961 aggaacgacc ttcctgatac aatttccaaa agaaaaataa aattaccagt aaaaaaccgt
     1021 ggagagatag ctgctattga tttttgctat gtcttttctt tgagaggtat ccggtatggt
     1081 ggagtgtctg aaaaataaaa aaatggaggc caattttgtg aaagctgtta tttctggaag
     1141 tattgttttc caagaagaat atcgagatat ggaaaaagat ttcagtaaaa aaggcattga
     1201 attaattgat tatcctcgct catgtccgat cctagacaaa gagtatccag aaatcttgac
     1261 gacttttttt taaatatcgc agcagcggat ttattttttg tatttaatca agatcggaaa
     1321 ggggtattag gctatattgg tgccgcaact ttttcagaaa tgaagtattg tctgatgcaa
     1381 aatcttattc atggaaaaag gatcaaaata gtcttattta aggaaccatc agaggaaaat
     1441 tttttgtttt gatgaggtag atcgctggct aaagaatggc tggatggaaa tcagacagcc
     1501 taacaatttg gcataacaaa ggtgtgggca aaaaactgct cacacctttt tgaatttttt
     1561 atttatatac tacaaaatca cttgaaaatt atgattcact tttgcttcga tgcgtgggtg
     1621 aatattcaaa tagtcggcaa ttaattcggt catgtcgatt tggatttctc ggacaatttt
     1681 ctccgcacca aacatcgcat aatttcctcc gccgattgcc cgatattgat ttgtcaccac
     1741 ttccagcatt tgttgcggaa gaacggggtg acctttaaaa cgaagttcgg taatccgttg
     1801 tccaacaggc tgtttcatat caatagtgta ctgaatccct tcatacatgt cataattgta
     1861 gtactggggc ttaggctgaa taaaggctgg attgaatacg atttggttat tttctacagc
     1921 taaataattt gcggtctgct ccaacgccgc tctcaaatct gcaccattta tttgaatcac
     1981 tgccagagta ttcggataaa tatagttggt gatgacgtcc cgcatcgtga tcgtctcacc
     2041 aaagcctttt ccttcattat tgaataacgc agttccagaa atctctgagc cgctggcaaa
     2101 taattgtaca cgattaataa actctacata gggatgttcc tcaagacgag cctgcatcgg
     2161 atcattaata cgcatatttc cgttcacttt acctaagggt tgatccaacc atttctctag
     2221 tttttcttct accggtacga tcgctgcaag aatttctgga tcaggctctg atttttcaac
     2281 tgagtgtaaa gcaacctggt gatctgtaac aaccacttgc ccctcttttt tctcgacggt
     2341 aagacgaatt tcaccgatga actcgccacg atagcctggt tgaacaacgg gaactccatg
     2401 aaccgtttca gcaattttcc ggtgctgatg accggtgaaa agggcgtcaa tgccttcaac
     2461 ctgctgcaat agctcgtagc cttcattctc tccagttagc gcttctgtgt cctcaccggt
     2521 taacaagtct ttttcaaatc cgccgtggta actaacgatt accaagtctg cttgtttcct
     2581 taatagagga acatattctt ttgctgtttc gacgatactc ttaaacgtaa ttcctgctaa
     2641 ggtttctggc ttttcccagt gaggaatgta ttgtgtcgtt aatcccaaga tagcaatttt
     2701 tacacccacc ttttcgataa tcttataggc gtttccaaag tatggttggt catttttgtt
     2761 caccatattg gctcctaata taggcgcttg ataattctta atcgcttcct cgagataatc
     2821 cacaccatag ttaaactcat gatttcccaa cacttgaaca tcatagctca tttggttaat
     2881 caagtctgta agctctttgg ctgaaccata ttctttagca agataataac tcaaaggaga
     2941 cccttggatg aaatcaccat tttcgatcgt aattaccggt ccatctgctt ttgcccgtaa
     3001 ttccttcagt ttcgacgata ctttagccgt tcctaaggcc agatcggttc tgccggaata
     3061 gtctgttggt gaaacatagc catgcatatc acttgtctct aaaaacgtaa tgttcattct
     3121 gcgtgctcct ttagctgact caaaatagaa aaaccgaagc gtattcagct gttcggtctc
     3181 tttttttgat ttataatgcc ttaatccttc atttgaagtg tttttaaaat gttgcctgga
     3241 ataaagtttt ctgttgtctg gcgaacaatc cttccttgat cgagatgata aaggttatac
     3301 ccatgacgtt cttcaaagcg tacgaagcca tcgccttcat cttcaccaac aaaggacata
     3361 ctcgatacag taaaataggg gatcccatta atttcatcag taaatgtgtg atgggtatga
     3421 ccgtttagga tacagcgaat atcgtaaggt gcgattagct tcaagaaacg ttcggtacca
     3481 ggccattttg gtatactgct ttggtgagac agtagatgat gatgggtgac caagaaaatc
     3541 ggttggtctt tttcttgctc taaggctttt ttcagccatt gaaattgctg atcgtccact
     3601 ataccatctg cgtatccata gcttgaatta tcaaaggaaa tcactgtaaa ctcaggatat
     3661 ttgactatct ggttgtacgg ggcagaagag gcggcttcat cgcaccagcc tacccaaaaa
     3721 tgttctttga tatcgtgatt gcctagagta acaagaatcg tcgtttctcc tagcgcattt
     3781 tccagccaag tcttcaagta tcgataatcg tctatttcgc catcttcggt taaatctccg
     3841 ctgataatca caaggtccaa gggtgtttgc tgcaggagat gatgaatgca ttgctccaga
     3901 gggataaggg gattctgcat cgtagccagc attcccttat agccttcttc acaagcctca
     3961 tactctctgc gaaagtgtat atctgatata tgcaatatat tcattcttat ctcaatctct
     4021 ttcttataat tccggttatt tgatctaaaa taaacacggt tacgactacg ccaatcaaaa
     4081 taatactcac tttgctccaa gaccgagttt gaatcgcgaa tatcaatgga gcaccgattc
     4141 cgccagcgcc aactaagcct aaggtagccg cactacgaac cgctatttca aaatgattta
     4201 atgctaaaga acaataaatc ggcatcaaat aggggatgcg aacaaagaaa atcgtttgcc
     4261 aaaaatttgc tcctaccgca tgcatttctt caacaagtcg ctcatccatc gcttccattt
     4321 cttctgtaaa aagtttgcca agcattccga tctgatggac gccaatcgcc ataacaccag
     4381 caaaggggcc aggtccgacg actttcacaa aaataattgc aaaaactagt tcaggaaaag
     4441 ctcgtaaaac attacagata agcttgccaa tttttgttac ccatggatgt gcttgccaga
     4501 ggttaaccgc actgatcaag gtaatgggaa tcgctaatac cgtagcgatc attgtcccta
     4561 aacaggcgat tccaatggtt aataatagca aactaatcaa atcctcgccg ctgccatcat
     4621 atagaaactg ccaatcaggc tgacctaacc ctctcaaaac gtccattccc atggaaggag
     4681 agaaggtgct taatccagag taatccagag tagcggccga caaaataacg agaatcacta
     4741 aagcaactaa tgataaaagg gttcttctgt tcatttcacg aagcatcata acagggacct
     4801 cctgacacgt tcacttaatt tatcaacgat cgcgacagtt actaggatcg ttagaataat
     4861 gattgaaact cgttcataat tcaaaagcgc aagtgaggaa tttagaataa cgccaatacc
     4921 accagctccg acatatccaa ggacagtaga agcacgaaca ttcacctcaa acgcgtaaaa
     4981 agcatagctc gcaaactgac tcaaaacttg tggagcgatc gaccaaatag cgatttgcat
     5041 tttatttgct ccaacagccg cagcggcttc aatcgggcca aagtcaatgg tttcaattgc
     5101 ttgaaacatt aattgtgaca ccataccaaa agtaaagaca gcgatagtca acacgccagt
     5161 cgcttcacca attccaaaca cagcaacaaa aagcgcggct aaaagcaggg tagggatggt
     5221 ccgtactaaa cctaaaagaa aacgaaagat ggtagaaaga agacggtttt ctgtcactac
     5281 agtggtcgct aaaaaactaa cagggatcgc taaaattaag ccaatgaccg ttccaagcaa
     5341 agacatttga agtgttttca ccataggctc gactaattta ggtaaatagc taaagtctgg
     5401 atgaatgaag cgtgataaaa agacagtcat ttgattggag ttttgaaaaa cctgagaaaa
     5461 atcagctgac gtgatccagg cacttccttg aaaacaaaca attaagaaca gaagaataag
     5521 caggtgttta taccatttaa agtgaacggt ttctttcaag ctcatcgatt tcgcctgcct
     5581 tttcttcaaa gattgcattg ccataaattt gctctaagcg ttcatcggtt aagtcgtctg
     5641 gacgaccatc aaagacaacc tgagcagctt ttaacgcgag cacacgcgta gaaaactcac
     5701 gagccagagg gactgaatgc agattgacaa tgactgtttt atccatcgtt tgattgatat
     5761 tttttaggtc catcataacc ttttgagtcg tgattggatc taatgatgca acgggttcat
     5821 ccgctaaaat aatgggggct tgctgcacga gtgctcgcgc aatggatact cgctgttgtt
     5881 gcccaccact gagctcatca ctgcgtgtct gcaatttatc agacaaacca actttttcca
     5941 gtgcttcctt tgtctgctgg tagtcttcct tcgtaaaaag acctaaaata cttttccaag
     6001 tggagtaata gcccaaacgt ccagatagca catttttctg aaccgtacta cgctttacca
     6061 gattgaagct ttgcgagatt agaccaatct ggcggcgaat ctgtcgcagc tcttttttat
     6121 tcgcttttgt aatagaggtt ccattaatgc gaatatctcc tttggtggtt tctgccaagc
     6181 gattgatact gcgcagcaga gtgctctttc cagcaccact caaccctatg atggaaacaa
     6241 attctccatc ttcaattgtt aagtcaatat ccttcaagcc aacaacgcca ttgggataga
     6301 ccttcgaaac attgtcaaaa ataatcatta tccttctcca tttttttaag tgtaagcaaa
     6361 aaaaagcttg ctgacactta ctgatgtttt attctgctgc ttttttcgtg tagtctctga
     6421 tcgtgtcata ggcagaatca gcagcttctt catagccctg atgcccccac aaagccattg
     6481 cttctttgcc atctttatca tccttcatat ttaagaaggt ctctttaatt ttcgctttta
     6541 aatcttttga catgtcaggc tgtacagcga ttgcgtcatt cggaatatcg ccttttgtca
     6601 ggtacaggac ttttaaatcc ttaaataaat cattatctgg gaaattcgaa gcaaaaacat
     6661 ttcgcgcgcc ttcaaaaaca aacgccgcat ccatttgctt gttcaacact gcggtaatct
     6721 cactcggaat atcattcaca gtggtcatct ttgtatctgc agtaggatca atcccagcat
     6781 ctttcaattc agcgacagga tagatgtagc cgctagcaga gttaggactt aaggttgcga
     6841 tattttttcc ttttaaatca gttaacttat tcaatccgct atcggcacga accaatactt
     6901 ctcctttaaa ggtgttcgta agtttgtcct ttaatggcaa gccagtttct tgatcataat
     6961 cgccgagctg agaagtcagg attgcatctg cagctccagc atcctttgct tgaacatagg
     7021 ctgcaggcgg cataattccg acatcgactt gaccagaaga catcgcttcg acgatcgttg
     7081 aataatcagt agctaacgtc acttctacct cacgatctaa ctttttcgat aaatacttag
     7141 caaatggttt tgcttttgcc tccatactac catcattgtt tgttggaacg aattgaacaa
     7201 ctaatttttc tttgctctct ttttctgagc tgctacttgt attcccagaa ccgcaggccg
     7261 ccaaagtggt caataacatg actccgatga acccttttgc taactgtttg aactgcattt
     7321 ttctcctcct gttggatata atttctaata cgtctaaatc atagcaggga atcaaaatca
     7381 atgaagtgac gcacgtccta ttttacacaa cttttacata caatttacaa acaattataa
     7441 ataattattc gtattgagcc gaaaaacaat ctaagctata ataaagaaaa acttcttaga
     7501 ggtgacgatt tcatgaaaaa aatccatgat acgaagcaat taactgctta cgttgagaag
     7561 cacaacctgc aggattatat ggattcagat atattggcaa tggcttcttt actttttttt
     7621 gaaaaggaag agcacctcat tcaaacggga aaaacctcag attatttgta ttttttagtg
     7681 gatgggacgg taatggtgta ctcgtattct tctgatacac aaaatatttg catcgattac
     7741 gcaaagcccg ctacaccact cggcgaagcc tcttcattgt gggaattatc gccgaaaagc
     7801 agcgttaaag cagtgactaa gtgcatctgt atcagcattc cgctaaatca atatcgaaca
     7861 cagcttcaaa aagatgttcg ctttttgcag aatatttgcc agttactaag ttatcgttta
     7921 aattccggga taaatttggc gaactcactg acagaacctg ttgaaactcg tttagcaaag
     7981 tttattttga cccatcacga aaacgaacgt ttctcatttc ggttaaccac ctgcgcctca
     8041 atcttaaatg tcagttatcg acatctttta cgaacaatca cgaattttcg tgaggagaat
     8101 attatcgaaa agaaaaagaa ttattacttg attcatgata tcgaagcttt agaggactta
     8161 gctgagaata taagtagtcc agtaaaagca gaataacagc tataaaaaaa cagcagtcag
     8221 aagtgattgc tggttttttg atagtggaga aattgggaaa cggaaaatct tcgttgaaga
     8281 gtagtgttct atatattggt atgctatact tttaaaagtt caatgtgtca agacacataa
     8341 aaaggggaga aaaattttga agaaaatact tgtttcattg ttgtttttgg tgtcattagt
     8401 aacgttagtc ggctgtagtc agtctcaggg ggagaagcaa agtgaaaaag agaaaagtaa
     8461 aatcgctttg gttcttggat ttggcggggt tgatgaccgc tccttcaatc aagctggctg
     8521 ggaaggatta gaggcttggg gaaaagagaa aaagctgaca gcaaaaaaag attttacgta
     8581 tatagaaacg aacaatccag aggataacag taccaatatc gatcaggcaa tcagaaacgg
     8641 ctttaccacc attattagct tgagctactt gcaagcagag gaactggaaa atgctgctaa
     8701 agataatcca aagttgaaat ttgccttggt agatggtgag attactggga aaaagaatat
     8761 ctcatcagca acctttaaag atcaagaaag tgcgtattta gcaggactag ctgccgcata
     8821 tacgacaaaa agtgatcaag taggctttat tggtggaatg gaagggcccg tgatccaacg
     8881 tttccaaagc ggatttgaag ctggcgttaa ggcaggggcc acagcactga acaaggaaat
     8941 tactgttaac gtacaatatg ctgcatccta tgattcgcta gataaaggaa aagctattgc
     9001 tgcttctatg tacaaagagg gagtcgatat tctttatcag gcggcaggaa gtactggagc
     9061 tggggtgttc caagaagcaa aagcccgcaa tgaaaacgac tatagcagaa aagtatgggt
     9121 gattggtgta gatcgcgatc aagcggatga aggggattat aaaatggaga acggcgagac
     9181 agggaatttc acgttgactt ctacaaagaa aaatgtcggt gcagaagtca aagaatttgc
     9241 agaaacttct cataacgata aatttcctgg cggcaagcat ttgacctttg gtttgaaaga
     9301 agaaggggta gatctgacaa aaggacaatt atcagaggaa gcctatcagg cagtttcaga
     9361 cgcaaaagag aaaatcatca gtggtgattt cagactgtag acaaagtcca ttttatggat
     9421 gatgtctaca gt
//
LOCUS       DAYYHX010000106         9375 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000106 DAYYHX010000000
VERSION     DAYYHX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.107"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            232..2067
                     /gene="tdc"
                     /locus_tag="ACHA6Y_001848"
     CDS             232..2067
                     /gene="tdc"
                     /locus_tag="ACHA6Y_001848"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8987935.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2372..3850
                     /locus_tag="ACHA6Y_001849"
     CDS             2372..3850
                     /locus_tag="ACHA6Y_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8987936.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3816..5148
                     /locus_tag="ACHA6Y_001850"
                     /pseudo
     CDS             3816..5148
                     /locus_tag="ACHA6Y_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            5262..7814
                     /locus_tag="ACHA6Y_001851"
     CDS             5262..7814
                     /locus_tag="ACHA6Y_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM8987937.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7821..9260)
                     /locus_tag="ACHA6Y_001852"
     CDS             complement(7821..9260)
                     /locus_tag="ACHA6Y_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8987938.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
BASE COUNT         2931 a         1562 c         2004 g         2878 t
ORIGIN      
        1 cgcttttttt aggttatcta ttttagaaga ctgatttatt gaaaaattgt tcgctttttt
       61 gcgttgaatt atcagaaaaa tccgacttta tttatttttg tttgtaaaaa tctacgtgga
      121 tttgatgtta taaaaaaaaa attctcattt ttggcgctat agtgaagagg tgttacagat
      181 aaacacatgt atctgcaaga cattgaccaa caaaatatgg agggaagaaa aatgaaagat
      241 atggatatca aggccgtctt tataggcgac aaagctgaaa atggaccagt ttataaaatg
      301 ttgttgaaca agatggtaga tgaacatctt ggatggagag aaaattatat tccttcagat
      361 atgcctgcaa ttagcgaagg agataagctc actccagatt atctagccac ccgcgaccat
      421 atgatagagg tcttggatga agtcagtcag cgattacgcg caggatcgat cccatggcat
      481 tcagcaggcc gttactgggg acaaatgaat gctgaaacat tgatgcctgc tttattagct
      541 tacaactatg cgatgctgtg gaacccaaat aatgttgcat tggaatcttc tatggcaact
      601 tcgcaaatgg aagcagaagt aggacaggat tttgcttcat tattcaatat gacagacgga
      661 tggggacaca ttgcagcaga tggttctatt gctaatcttg aaggattatg gtacgcccgt
      721 tgcatcaaat cgattccttt agctgtcaag gaagtgctgc ctgaaaaagt gaaaaaaatg
      781 tctgagtggg aacttttgaa tttatctgtt gaagaaattt tggaaatgac cgaaagcttt
      841 acggacgaag aaatggatga ggtcaaagca gcttcttccc gtagcggaaa aaatatccaa
      901 agattaggga aatggctggt ccctcaaaca aaacactatt cttggatgaa ggcgttagat
      961 atttgcggag tcggcttaga tcagatggtt gctatcccag ttcaagagga ttatcgaatg
     1021 gatattaatg ctttggaaaa aacaattcgt gaattagctg gacaaaaaat cccaattcta
     1081 ggtgtggttg ctgtcgtagg aacgacagaa gaaggtcaag tagacagtgt agataaaatc
     1141 gtccaattaa gagaaaggtt aaaagatgaa gggatctatt tctatttaca tgttgatgcc
     1201 gcatatggcg gctatgcgcg ttcactgttt ctaaacgaag caggagaatt tgtgccgtat
     1261 gcttccttag ctgaattctt tgaagaacat catgttttcc accactgcgt aacgattgac
     1321 aaagaggtat atgaagggtt cagagctatt tcagaagcgg attctgtcac gatagatcct
     1381 cataagatgg gctatgtccc ttatgctgct ggaggaatcg taatcaaaca taaaaatatg
     1441 agaaatatca tttcttattt cgcgccatat gtgtttgaaa aatcagtaaa ggctccagat
     1501 atgttaggag cttatatttt ggaaggatca aaagctggag caaccgccgc cgcagtctgg
     1561 acagcacatc gtgtattgcc attgaatgtg accggatatg gtcaattgat tggggcttca
     1621 atcgaggcag ctcaaagatt cagagaattt ctcgatcatc tgacttttac tgttaaagga
     1681 aaaacaatcg aagtctatcc attgaatcat cctgatttta atatggtcaa ttgggtattc
     1741 aaagaacaag gctgtacaga tttgaacgct atcaatgaat taaatgaaaa aatgttcgat
     1801 cggtcttctt atatggatgg cgatgtttat ggtgaacggt ttattacttc tcatactaca
     1861 tttacacaag aagattacgg tgattctccg attcgttttg ttgaaagaat gggtctaacg
     1921 aaagaagaat ggaaaaaaga acagaaaatc actctgctac gtgcggctat catgactcct
     1981 tatttgaatg atgatcgaat tttcaatttt tatacaaaaa aaatcgccaa agcaatggaa
     2041 aagaaactaa acgaaatcat ccaatagaac tgatttttgt gtgaaagaat aacaagagac
     2101 atatggagaa cagacaatta tttgcttcat caaaacaatt gtatttcccc ctgtttttca
     2161 tatattcctc gccaagatat tcttttttat aaaacgttct ataagttgga tcgttcgttt
     2221 catgtatcaa acccagttca ctaagatcgt tcaatggatg tttcggtgat actgggtttt
     2281 gtactgaatc gatgaaacct tgtcatttct gttcttgaac aaaaataaaa gaaaacaaaa
     2341 acatatgtcg ctattgaaag aaggaataaa gatgaaagat actggttcat caaagcaaat
     2401 cacgaccatg acctttatcg gaatgacctg cgcccttgta gcaagtgtac gaaatattcc
     2461 agatgtagca gcaacgagct ggacgatgat tttttacatg ctggttgcag tattattgta
     2521 cgcttttcca atctcattga tctctggtga atttgctgga atgtttcccc aaaaaggtgg
     2581 tccagaactt tgggtatcga atgctctagg aaaaaaatgg gggttcgttg tctcatggct
     2641 gttatgggtc caaatgtttc ctgggatggt gatggtcgct tcagcactcg ctccgttgtt
     2701 tggtaatatt atcgataatg tgccgcttgg tttgaacagc aaatttacat tagttgtcat
     2761 tttagtcgtt tattggatca tcactttttt gaatttgaaa tttgatatgg caaaaatcgg
     2821 cggaaaagtc ggtgtttggt taggattgta cgtgcctttg acgatgatgc tcttattagg
     2881 gtttgctgcg tggatcaaag ttgggatcgt accaaccgga acattaggca gtttttcatg
     2941 ggacaaatta ataccagata ctacgactgc atcctcattt gtttattttg cgccgatcat
     3001 gtttattttt actggtatcg aaatgtcctc tgtgtatatc actcgattgg aaaatccagt
     3061 taaaacgtat attcgcggag tatttgccgc gttaattttt atatttttcg tcaatatttt
     3121 gaatgcttta gttgtagcaa atgtcgtacc aaaaggacaa atggaattga ataatatcgc
     3181 gcaatctatt tcgatttatt gccaaattct tggtttgcca catcttattg tgaatctgtt
     3241 tagtttactt gtcttcattg gtgtagcagt ccaattatct gcttgggcct caggaccagc
     3301 aaagacagtg acagaaagtg ctagaaaagg tgcgtatcca ccaaaattca atttttggaa
     3361 gacgaatcaa tttgacgtat ccaaatccgt tattttgact cagtctgtca ttatctcaat
     3421 ctttgcactt ttttatttgc tgattcctgg cgtcaatcaa gcatttttga tgctcgtaaa
     3481 ttcaactact gttatctact gtatcgtcta tgtcatcatg gggatcgcag ttttgagatt
     3541 gcgttataca catgcaaagt tgaatcgacc atttagaatt ggaaaaaaag agaaaaagag
     3601 caatcttgga gtttggactg tcgttattgt tttatttgcg gcaatcggat tttctgtcgg
     3661 actgacgatg aaagctggaa catggatcaa tctaattgca gtcacagcca tcagtgtcat
     3721 tttgtttgta gtaccactct gtattgaaaa aataaagaaa ccttcttggg aacaggaagt
     3781 cgccatgctg cttgaaaaag aaaaaggtga acttgatgga aaaaccggca atgaaacgga
     3841 ctctaactaa tcgaaatatc cagttaatcg cattaggtgg cgcaattgga acaggattat
     3901 ttttaggttc agcaaaggca attgcatcag cagggccagc tgttattctc gtttatttga
     3961 tatgcggttt ggctatttat ggtttgatgc gggccatggg tgatttattt ttagcaaata
     4021 gtacatttaa ctctatcagt gatttcgttg actattattt aggcaaaaag tgggcatttt
     4081 ttgtcaattg gacttattgg ctttgctggc ttggagctgg acttgcggaa ttaacagcaa
     4141 taggattgta tatccatttc tggtttgtac atgtaccagc tttgataagc ggaggtagcg
     4201 cactaatgat cttaatggca gtcaaccttg ttgcagtcaa gtggtttggt gaattggaat
     4261 cttttttctc aatcatcaag atcttgggga ttttcctttt tatcttattg ggtataggat
     4321 tgagtgtctc agattttcat acccagtcta tcggaatcaa agaaaatttt ggacagatcg
     4381 atgaattact accgtttggc tttgctggaa tggtagtctc atttcctatg gttttattca
     4441 gttttgctgg aattgaaatg atcggcttaa ccgttggcga gacaagtgat ccgcaaaaga
     4501 atttgaaaaa agcaatcaac tgtctgcctt ggcgtattct ctttttctat ataggaacga
     4561 ttttgtcgat tttatttgtt gttccatggc attgtttaga tttaaaagaa agtcctttag
     4621 tgactatgct gcaagtgatc cattgccaag gcggagcagc aattgtcaat ctaatgattt
     4681 tggttgcttc tttgtcctca gcaaatagtg cagtctacag tactagcagg attttatatc
     4741 aaacagcaaa agaaaaaagt ttgccccatt ggttttgtgc gctttctaag aaaggcgtac
     4801 ctttacacgg tattttaatt acaggaatgt tatttttttg ccggtctttg tctacatttt
     4861 tttgtagctg atagtcgatt gttttttcag cttttggcag gcatgacaac gagttgtttt
     4921 ctatttgtgt ggtcttcgat cttgtgtgca catgttcaat ttattaaaga aaagaaacgg
     4981 aacactacac aaagatataa agatcttgct ttgttggtgt tttttacttc aattgctttc
     5041 agcttgttat tcgaaagcac gacacgcttt attccttttt tattggttat ctggtttgta
     5101 ttcttaaatt ttgtatataa aagaataagc aatagaaata tcagttaggc ccagatacat
     5161 tttgcaaaac gaattatttc agacccccct gaaataatcg atacgcctga aaaaacttcc
     5221 ctgcgtataa taacagtatc aattaaacag gaggggatag gatgaactgg tacagattga
     5281 cggtagaaga tgtattgaaa aactatcaaa catcaacaga aggcttgtcg caagaagaac
     5341 gacagaaacg cctgactgat caaggatata atgaaataaa agcaaggcag cagacaaaac
     5401 gatggaaaaa aatagcaaag cattttactg atctgctgat gatcgtgttg atcattgcct
     5461 cttttttgaa atttgcttct agtgaatata ttgaaggaag tattatttta ttcgtagtga
     5521 tcgtcaatgg actcgttagt tactggcaag aaagaaaagc ggaagaatca ttgaatggtt
     5581 tgaagcagct gatgggacaa gaagcgatag tactatcaaa cgggatgcaa gaaaaaatcc
     5641 ctgcgaaaga actggtaaaa ggagatgtca ttgtcttacg tgccggagat gtcgttccag
     5701 cagatatccg attaatcgaa gcacatgatt tattagtgga agaatcgatt ttgacgggag
     5761 aatcagaagc atctgaaaaa agtcatgccg ttttgacaga agaagaatcg atcggcgacc
     5821 agaaaaatat gggtttttca ggaacgcttg tacaatctgg ttctgcagtc ggtgtcgttg
     5881 tagaaacagg aaacacgaca gaaatcggta aaatcaatca agcattgcaa tcggtcgaac
     5941 aacaaacgac gcctctgatt aaaaaaatca accgattaaa caaacaaatt tttcaaggca
     6001 ttctttgttt gagcctattc ttagttattt ttacgacatt tcgatatggt atggattggc
     6061 atattcttct ttcggctacg attgctttgg tggtgtcaat ggtacccgaa ggattgccag
     6121 ctgttttgac catgattcta tccgttggag tacatgaaat ggcaaaagaa aaggcgatca
     6181 tcaaaggact tccttcggta gaaacattag gttctatgac agtgatctgt tcagataaga
     6241 caggaacatt gacaaaaaat gaaatgacag ttgtggatgt agctgcaaaa gaagaagcct
     6301 gcgtgttgtc cattatgaaa aactgtcagg aattaaaaac aaaagaagaa cagaaaacag
     6361 aaaatttaag tggcaatccg acagaagttg cgctgattcg acacacagaa acagccaatc
     6421 tacctttgaa aaaaatagaa gcaaagattc cgttcagttc tgaatacaaa tatatggcaa
     6481 cgatgcatgc ggaggaaaaa ggagcggtga tttacgtcaa aggtgcacca gaagtattat
     6541 ttgctaaatc aactctttct tctgcagaac aagaagcatg gagtcagaca gctgcagaat
     6601 ttgcgagaaa aggtcagcgt gttttaggat ttgcttataa aaaagtagat tcgaaacagg
     6661 aactcacgca tgagacttta acccaattaa cttttgcagg gatcgccgga ttgatcgatc
     6721 cgccaaaaga aagtgcagtc aaagctgtaa aggaatgcca acaagcaggg atctcagtga
     6781 aaatgattac aggggatcac aaagacacag caaaagcgat tgcggaacaa attggcttaa
     6841 aacatactgc caaagtttta gaagggatcg atttggatct tatgtctgat gaagagttga
     6901 ttcagcaagt gccaatagtc gatgtctttg ctcgaacgac accagaacat aaactgcgaa
     6961 ttgttaaagc tttgcagaaa aatggagaaa tcgtcggaat gaccggagac ggcgtaaatg
     7021 atgcacctgc tttgaaaaga tccgatgtag gaattgctat gggaatcaaa ggtagtgaag
     7081 taagcaaaca ggcagctgat atggttttag gagatgataa tttccatacg attgcaaaag
     7141 ccgtaaaaga gggccggcgg attttggata atttgcaaaa aaccatcaat ttcttcttgc
     7201 caactgcatt agctcaagga ttgatcttga tttgggcact tatgctgaat cgcccgctac
     7261 ctttgtcccc agtacagatt ttatgggtca atatggtaac gacaatcact ttatcttatg
     7321 cactagggtt cgagtcgcca gatccagaaa tcatgaagcg cccaccaaga gatccaaagc
     7381 aaggaatcct atctggttat catttgtttc ggatcatcta tgtctcccta ctgatcatgg
     7441 ttcctgccta tttattggct atgcagtttg aagggcaaag cttacagcaa accattcttc
     7501 tgcaaaatat cgtcttagct caagctgttt acatgatcaa ttgcagagag ttgtccaagc
     7561 cgtcattgaa ccaaggtctt ttgcaaaaca aagcgctttt tctttcatta ggtatccttg
     7621 ctttattgca agggatggtt atttttctac cagtcggcca gcagctgatc ggtacagtaa
     7681 gtttaacgat gacacaacag ttattgattg gagcaaatgt gattcttcta ttcttagttg
     7741 tagaaataga aaaagggatc atgaacaaac tgtttcgcaa aaaagaaaaa acagctagag
     7801 aatacagtcg ctagccgtta ctataaaaaa gttttgaaag tgagttcagt caaacgattg
     7861 agctcatttt tcgttggaat agaagaaatg tatacaatag ggatatctag agaatctaga
     7921 gggaaattgg aaatcaagat atctgttttt tcaaaagaaa tagtggaaag atcggttagt
     7981 tcgataggta agaagcagac acgttttcct aagtaatctt ttaattcctg ctgcaagaac
     8041 gccttccatg aaggttctcc ttggaaaata aaatagacat tgaacaaatt cggtttgata
     8101 gaaaaaatcg tttgttggat cagtagagac aagagttgga gaaagtaatt tggttcattg
     8161 atttctattt ctttttttag atcgttcagt aaattctctt tctgttgata caagtgtgca
     8221 tattgctgat catatgtttc taaaaaacga tggtattgac ggtccaatgc ttcaatcaat
     8281 atcggcgatt catgatattt taatatgagg agagccaagt tatcagtcag agatgttttt
     8341 tttagttgat caagagacag ctctgaagct agttcctcta caaacgtttc gaggacctct
     8401 aaaaatggag aatgatgcag gcagcgagcg gtttcttgtc catagttatt gttgtaattc
     8461 caactttcca gaaagcagaa agaagcaaaa gaaagctcag tgtctttaag ataaatacct
     8521 tccgttaaat acaatttttt caatgcagag tcatacaggg aggagagtgt gctgttggta
     8581 tggatagaag aatctaatcg gcaattcgct tttatacgga tcgtattgat aaagaaccaa
     8641 catgttccga aaatctcttc tgtttcaacc gctagttcag tttgactgag gtttcttaaa
     8701 aattgaaaat aatgggattc atggatcgaa taatcctcga aaaaatagtt gttatgagtg
     8761 aaaggaatca gcaatcgatg ataaaaagta cgaatcgcac gctcttttcc aagaatacaa
     8821 gctgtatatt tcgtcaaatc gatctgcaag tcgtaacttg ctaaacgctg attgatttta
     8881 gtcacatgtc ttttcaatgt cccataagaa agcccatggt cggctagaaa atcagggaca
     8941 gcaacttttt ttgtaaaaag cagatttttc agcagttcga cactgactga ttgtttgaaa
     9001 aaaatctccc aaaggtcatt tgttaataaa ttttgctggt tttgcaaact gattcctgct
     9061 tgatcgctga tgatgtccca gccttcgggc agctgagagc gaatagattg aaggtcagaa
     9121 aaaatcgttc gttcggttgt ccctatgatt tcagctaatt catttacggt tatctttgta
     9181 tgattcagca attgttctac taaagaaaca tgacgtcgaa tatctttttc ggtaatgatt
     9241 tttttcatta gtgaatacat caatcagact cccttctatt ctaagaatac ggagaaaata
     9301 aacggtggtc aagtttcaac tgatttttca atcattgttt atttttctgg agcatttact
     9361 gatgataaga atttc
//
LOCUS       DAYYHX010000107         9110 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000107 DAYYHX010000000
VERSION     DAYYHX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.108"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            214..438
                     /gene="nrdH"
                     /locus_tag="ACHA6Y_001853"
     CDS             214..438
                     /gene="nrdH"
                     /locus_tag="ACHA6Y_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM8987939.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            597..2756
                     /gene="nrdE"
                     /locus_tag="ACHA6Y_001854"
     CDS             597..2756
                     /gene="nrdE"
                     /locus_tag="ACHA6Y_001854"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM8987940.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2806..3771
                     /gene="nrdF"
                     /locus_tag="ACHA6Y_001855"
     CDS             2806..3771
                     /gene="nrdF"
                     /locus_tag="ACHA6Y_001855"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM8987941.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3860..4999)
                     /locus_tag="ACHA6Y_001856"
     CDS             complement(3860..4999)
                     /locus_tag="ACHA6Y_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8987942.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5308..6021)
                     /locus_tag="ACHA6Y_001857"
     CDS             complement(5308..6021)
                     /locus_tag="ACHA6Y_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM8987943.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6275..6976)
                     /gene="nagB"
                     /locus_tag="ACHA6Y_001858"
     CDS             complement(6275..6976)
                     /gene="nagB"
                     /locus_tag="ACHA6Y_001858"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM8987944.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7210..8742
                     /locus_tag="ACHA6Y_001859"
     CDS             7210..8742
                     /locus_tag="ACHA6Y_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8987945.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8933..>9110)
                     /locus_tag="ACHA6Y_001860"
     CDS             complement(8933..>9110)
                     /locus_tag="ACHA6Y_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8987946.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         3033 a         1775 c         1591 g         2711 t
ORIGIN      
        1 aatatttctt catagttttc cacaattttt tctctattaa atttgctatt ttcccacacg
       61 atacttgttt tcagctcttt tttcaaaaac acaaaactta gtattccaca gtttccacaa
      121 cacaatatgt agtgtttgtt gtttgacttt tttactaaga gttttatact atctattata
      181 gataataaga atgtgttaga ggaggcactc accatgaacg taaaaatctt ttctaaaaat
      241 aattgtatgc aatgtaagat ggtcaaacgg tttttaacag aaaatcaaat tgcttttgaa
      301 gaagtcaata tcgatgaaca gcctgaagca gtagactggt tgaaagcaca aggattccaa
      361 agtgttcctg tcattacttc tgatgcagaa acagttatcg gattccgtcc tgatcagttg
      421 cgtaaacttg ccagctaaca tgactaaaag aatcaaaacg tgatacatgg atttgaaggg
      481 cttgagacag caccagtttt tgtcgcaaac ttccaaatcc ttttttaaag cttaaaaaca
      541 atgcaatgat ttgcaatcaa gcatcatcaa atacgaaatg aaggttgggc gtacgtatga
      601 gtcttaaaaa tttacaagat gtcagttatt tcaagctgaa taatgaaatc aatcggccag
      661 tcaatggtca aatccctttg aataaggata aagaagcttt agttgccttt tttgaggaaa
      721 atgtcaaacc aaatacgatg acttttccat ctattatgga aaaaatcaat tacttgatcg
      781 atcaagaata tatagaagca gaattcattc aattgtattc accatcattt attgaaaagc
      841 tatatcaatt tttggacgag caacattttt ctttcaaatc ttttatggct gcttacaaat
      901 tttattctca atatgcgttg aaaaccaacg acggttccgc ttatctggaa acttatgaag
      961 atcgagtagc cttcaatgcg ctttactttg cgaacgggga tgaagaatta gcacttgctc
     1021 ttgcagacga aatgatccat caacgatatc agccagctac accttctttc ttgaatgctg
     1081 gacgtaaacg tcggggcgaa ttggtgtctt gtttcttgac tcaagtcact gacgatatga
     1141 actctatcgg gcgctcaatc aactctgctt tgcaattgtc ccgtattggt ggcggcgtag
     1201 ggattacttt atcaaatctt cgtgaagccg gcgctcctat caaaggatat gagggtgctg
     1261 caagtggtgt cgttccagtc atgaaattat tcgaagatag tttcagctat tctaatcaat
     1321 taggtcagcg tcaaggagct ggcgtggttt atttgaacgt tttccatcca gacattattg
     1381 ctttcctttc agcaaaaaaa gaaaatgcag atgaaaagat tcgcgtaaaa actctttctc
     1441 ttggcgtgat cgtacctgat aaattttatg aattgacacg taaaaacgaa gatatgtatt
     1501 tatttagtcc atatagtgtc gaaaaagaat atggtgtccc attctcttat gtaaatatta
     1561 cagctgagta tgatcatcta gttgcaaatc caaacattcg caaacaaaaa atcaaagcgc
     1621 gtgatcttga aaatgaaatc tcaaaattac aacaagaatc tggctatcct tacgtaatca
     1681 atattgatac agcgaaccgc gagaatccaa tcgatggcaa gatcatcatg agtaatttat
     1741 gttcagagat tttacaagtt caaacgcctt ctgtgattaa cggcaaacag gaatatgaag
     1801 tattaggaac agatatctca tgtaacttag gctcgacaaa tatcgtgaat ttgatgcaaa
     1861 gccctaactt tggtcaatct gtgcgtacga tgactcgtgc attgacattt attacagatg
     1921 catctgatat cgatgtcgta ccaccgatcc aaaacggaaa taagctgaat catacgattg
     1981 gattaggtgc catgggctta cacacttatc ttgcaaaaga acaaatggaa tatggctcag
     2041 aggattcatt agactttaca aatatttact tcatgctgtt gaattattgg acacttgttg
     2101 aaagcaacaa tatcgctcgt gaaagaaaac aagttttcca taattttgaa aaatcagctt
     2161 atgctgacgg cacttatttc gaaaaatacg taactggaga attccagcca aaatcagaaa
     2221 aagtcaaagc attgtttgaa ggcattttta tcccaactgc tgaagattgg aatgctttga
     2281 aacaagcagt aatgaaagat ggactgtatc atcaaaatcg cttggcagta gcaccaaatg
     2341 gctcaatttc atatatcaac gatacgagtg caagtattca tcctatcact cgcatgatcg
     2401 aagaacgcca agagaagaaa atcggtaaaa tttattaccc agcgccttat ctatccaatg
     2461 agacgattcc gtattacact tctgcttatg atatggacat gcgtaaagta atcgatgttt
     2521 atgcaacggc acaacaacac gtcgatcaag gcatgagttt gactttattc atgcgttctg
     2581 aaatcccaga aggcatttat gaatggaaga caacaacgaa gcaaacgaca cgtgatttga
     2641 atattttaag acattacgct ttccacaaag gaatcaaatc catttattac gtacgtacct
     2701 ttactgatga tgcagaagaa atcggaagca atcaatgtga aagctgcgtg atctaatatc
     2761 atagatagat tcgcttattc atattttaat taaaggagca ttctcatgtc agaaacatat
     2821 tatgaagcaa taaactggaa tgaaattgaa gatatcattg ataaatccac ttgggagaag
     2881 ctgacagagc aattttggct agatacacgt atcccacttt caaatgacct agatgattgg
     2941 cgtacattgt ctcaattgga aaaagataca gtaggacatg tatttggcgg tttgacactg
     3001 ctagatactg tccaatctga aagtggcatg gatcagttgc gtaaagatgt ccgcactccc
     3061 catgaagaag ccgttttgaa caatattcaa ttcatggaat ccgttcatgc caaaagttat
     3121 tcttctattt ttagtacact gaatacgaaa aaagaaatcg aagaaatttt tgactggaca
     3181 aatacgaatc cttatctgca aaaaaaagct gagcgtatca atgaaatcta caaacacggt
     3241 acaccgctag aaaagaaaat tgccagcgtc tttttagaaa cattcttgtt ctattcaggt
     3301 ttttatactc ctctttatta tttaggaaac aacaaattgg ctaacgtagc agagatcatc
     3361 aaactaatca tccgagatga atctgtacat ggaacctaca tcggttacaa attccaatta
     3421 ggattcaacg agttacctga agcagaacaa gaaacgttaa aagactggat gtacaatctt
     3481 ctttatgagc tttacgaaaa cgaagaacgt tatacagaag aattatacga tccaatcggc
     3541 tggacagaag aagtcaagac attcctacgc tataatgcca acaaagcatt gatgaaccta
     3601 gggatggatc cactctttcc agatactgcc aatgatgtca acccaatcgt aatgaacggg
     3661 atttcaacag gcacaagcaa ccatgacttc ttctcacaag tcggaaacgg ctatttgtta
     3721 ggtcatgtag aagcgatgaa agacgacgat tatttgattg gattggaata aggatttatt
     3781 ttaattcaaa aatttttcga aaaataaaaa gagtttagcg tacagatatt ttaaatgtac
     3841 actgaactct tttttatgct tattcacttg tttcttccag cacttttgga ctgtctaact
     3901 tttccgaaac atgttccatt cttctcaata aatagatttt ccggatattt aatattatcg
     3961 tcttaatcac tgcatagaca ggtatgcaaa tcagcatgcc taatattcct gcgacacttc
     4021 ctgctccaat caataccaag atgatcgtca aaggatggat attcattgat ttcccaaata
     4081 acaacggctt gatcaaattt ccatctaatt gttggatcac taaaatcgac aacgccatat
     4141 acaaagcttg aaatggagag ataaacaaac cgacaatgat tgctggtacc gctccaataa
     4201 aagggccgac atacgggata atattcgtga ttccgcaaat gaccgcaagc aacaagctat
     4261 aaggctgatc gaatattctc attgccaaaa agctcattac accaataatc aaagcatcta
     4321 agatcgtact gctgatatag gcagataatg tttcgtttaa ttcttttacc gtttggcgta
     4381 actctttacg aatcgctttc ggaaagaagt gagacaacgc atcaataaat ttgtgcccat
     4441 ccttgaacat aaatagcaaa ataaatggaa cggtaaataa caaaataaaa aattgcatca
     4501 taaatgaaat gatttttgag acacttgcag ataaactgac taatatgata ttgaacaaat
     4561 tactaagaga taaatttgct gatgctaatt gttcttccaa attgaaattt ttaaactctt
     4621 cccgctgcgc caattcattt aaccatttgc ttgtttcatc tgcatacatt ggcaagctct
     4681 gggtcaattg aattgtctgt tccattaact gaggcactac attcatgaca gttaaaacaa
     4741 ctaaaatgat tacgaccaaa aacgtcaata aaaaaccgaa cactctcggg atccgatgct
     4801 tttcgagaaa aacaacgatt ggatcgaaca tataatagat aaaaccagca acaagcaatg
     4861 gtaaaaaaat tgccgaaagc attttcaata ttggactaaa aatattcggc atttgtaaaa
     4921 taaagtacac gccgatgatc gtgagtacta gctccactgt ccagaaaaat aattttgatt
     4981 gtttaaaacg tgattgcata catctcctcc aaggtccttt gatacttcta ttctatcatt
     5041 taacaaagag ttacgcgctg agattttcat ttgcttaaaa aaaacaaaaa aaataaacat
     5101 attttaatga gtagacgagt acttaaacaa aaacacggtt tggagtagtg acaaaaatta
     5161 tactgcagtc cctttttcca aataaacggt gttcaaaagc ttgcatcttc gtaataagcc
     5221 cagccaaacg aaaaatataa ggaactattt ttatttggct gggcttatta cttgatgtag
     5281 gacatctatt ttacaacctc agatcattta ttgcgcaata ctgactaaat gcaactttgt
     5341 gatatttttg tgtacggaca actcctcgat cacatcggca taagtgagcc cttttggaag
     5401 atcaatcgta tagatatttt tgtacactcg gtagtcatca acgaatttta cgtcaaaatt
     5461 cacatcttcg attacgatat ttttctcttc aaaataatcg gtaagaaaat ctttggtttc
     5521 ttttctatgg ataaattgga tttctaactt tttagtcgta ggtacatgga tcacgcgttt
     5581 cactactgtg agtgcaatag caatcccgat aaaactgatg atcgcaatgg aatagtaacc
     5641 catgccgatg gcgatcccta atccagcaat tgcccataaa gaagcggcag tcgtaagtcc
     5701 cgtcaccgat tgtttcgtga cgatgatcgt tcccgcgcct aagaaaccga caccactcac
     5761 cacttgagca atcagccttg cttcatctga acgaatcgtt ccagcaagct gtggatactc
     5821 taaagcatct cttaatgcac cactagcgat ctcaacttgg atcaatgcaa tgatacatgc
     5881 tcccatacaa actaaaatat gcgtacgcat acctgctgga cgatttttat attgtcgttc
     5941 aaaaccgatt gctccgccaa atagcatagc gatccctaaa cgtaaaataa tctctggaat
     6001 tgtcaatacc atatcattca tctaaacacc ctccagtcca atatatcatg tctgaaagga
     6061 gcagacaaaa aaagtgcaat ttttacctct tacgaaaaaa ggactgtgac aaaatttttg
     6121 tcatagtcct ttttatgaat aaacggtgtt caatcagctg accctcggta ttactcaaca
     6181 aacggcaaaa tatggggagc tatttttcct ttgtcttctc taatactcag gtcaaaacgc
     6241 tgatttcaca acctctttag cctactattg aaaattacaa cttacttgct gcttcttcat
     6301 cgatgatcac aacaacattg tcgtgatttt gcaatacact tgctggcaaa tgattcgtta
     6361 caggtccttc aatcatccct ttgattgcat cagctttgct ttcgccaaat gccatcaaga
     6421 cgatttcttt acttttcata attgaaccga ttcccattga aacagctttt gttggtacat
     6481 cttctgcttt atcgaagaat cgtttgtttg cattgattgt tgattctgtt aagtcaacta
     6541 ctgaagtcgt tccgtcaaat gaagcaccag gttcattgaa accgatatgt ccattttgcc
     6601 cgattcctaa gatttggata tcgattggat gtgcatcgat gatttgatca tactttttgc
     6661 atgctgcttc cagatcttct gcttttccat ctggtacaaa cgtttctttg aatggttttt
     6721 tatcaaataa ttggacatcc atgaaatgac ggtagctttg ttcatctgaa ccgcctagtc
     6781 caacgtactc atctaagttt acagaagtca tttcactgaa atccaaatcg cttgaagtca
     6841 tttctttata taaagtaact ggtgtgcttc ctgttgccaa tcctaatact tttgcacctt
     6901 tgttcatacc ttctttgatg atttcaaatg ctttttttcc gccttcttca gcgtttttta
     6961 cacgaatgat ttccatagtg attgctcctt tatattttgg tatagcccaa ttatagcatt
     7021 ctttttttct ttttacaata attagtatag accaaattgc atttttttca ttttcttgac
     7081 aataaatagg aatccgtgtt aggtttcata agatggttca atcaaacaat ccatgttgca
     7141 tgaaaaccaa gcaagtgatt cactgtggac gctttatgtc tgcagttttt ttatgaaaaa
     7201 ggagttatta tgagtatact gacattaaac aacattaccc agcaatttgg cgacaaaatc
     7261 ctctatgaag gtgtcgatct gcaattaaat gctggcgaac atcttgggct gattggacaa
     7321 aacggagccg gtaaaagtac attgattaaa atcatcactg gtgagatctt accagatgac
     7381 ggacagatcc attggcaaaa aaatattcac aaaggttacc tcgatcaata tgtggaagtc
     7441 gatgaaacat taacaatcga agaatttcta aaaactgcgt acgcaaaaga atacgaaaag
     7501 gaagaaaaaa ttgcttttct ttaccaaaca tatagcgaga ccttcgatga ttccttgctg
     7561 gaaaaagctg ggaaactcca aacggaatta gaccaaggag tgttttatca aatcgacacc
     7621 ttagttgctg agatgagcag cgggttgggg atcgatgtct taggtttaga ttcactgttg
     7681 aaaaatctaa gtggtggaca acgttcaaag gtgattttag caaaactact tttagaaaac
     7741 ccagatgttc tgatcttaga tgaaccaacc aatcacttag atgatcagca tatccaatgg
     7801 ctgacgcagt ttcttcagga ttttgacggt gcgtatatcg tgatttccca tgaccaagaa
     7861 tttcttgatc agatcaccac ccatatcgca gacatcgagt ttggaaagat tactaagtat
     7921 actggccatc taaaacaagc attgaaacaa aaggaacaaa atcgcgaaag ctatttgcgc
     7981 caatatcatg ctcaacaaaa acaaatcgaa aagaccgaag cttatattcg aaaatacaaa
     8041 gccggttccc gatcaacgat ggcaaaaagc cgacaaaaac aattagataa aatcgaacgg
     8101 ctgacgcctc cagcttcagc aagtaaagct gtctttgatt ttccattctc gcctattgtc
     8161 acgacattag cagtggaaac aactgattta gtcatcggat atgatcgacc gctacttgca
     8221 cccatcaatt tgaccatccg ctttggtgaa aatattgcca ttcgaggctt caacgggatt
     8281 ggaaaatcta ctttattaaa aacactgatc ggtgagattg aaaaaataag tggcgatttc
     8341 cacttcccag ataacacaaa aatcaattac ttttcacaag atctgtactg ggaaaatcca
     8401 ttagaaacac cactacaata tttaagcagt caatttccaa aagcgactat caaagaatta
     8461 cgtcgccaac tagctaaagc agggttagtt aatcaattag caagtgaacc tctcactaca
     8521 ttaagtggtg gggaacaaac taaggtgaaa cttgcacagt tgacaatgaa ccaaggtaat
     8581 ttactgattc tcgatgaacc gacgaaccat atcgatcaag aaacgaaaga aagcttacaa
     8641 gacgggatac agaaatttcc aggaacagta atcatcgtct ctcatgaaca agaattttat
     8701 caagatcttg tgcaacgagt cattgagatt gaaccaaaat aaacgtttga ttgaatagtt
     8761 gaaaaaaagg agctgtgaca aaagttttgt cacaactcct tcttctgaat aaatggtggc
     8821 caattagctg accttcggta ttagctcaaa aaacggcaaa atatgaggag ctatttttat
     8881 ttggctattc ttattacttg atgcagaaca gcttttcaca acctcttttc gtctattttt
     8941 tattttcgag atttctcttt cctttaaaaa caactgtttg aaagaagaac gcacatcctt
     9001 gaaaaacaag cccgctaacc gttacaccta accagccata ctgctgccac attgtcgtcc
     9061 ctagcaatga accagccgca cctccaataa agtacataaa cataaataca
//
LOCUS       DAYYHX010000108         9017 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000108 DAYYHX010000000
VERSION     DAYYHX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.109"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..1138)
                     /gene="recA"
                     /locus_tag="ACHA6Y_001861"
     CDS             complement(89..1138)
                     /gene="recA"
                     /locus_tag="ACHA6Y_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM8987947.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1247..2488)
                     /locus_tag="ACHA6Y_001862"
     CDS             complement(1247..2488)
                     /locus_tag="ACHA6Y_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM8987948.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2728..3306)
                     /gene="pgsA"
                     /locus_tag="ACHA6Y_001863"
     CDS             complement(2728..3306)
                     /gene="pgsA"
                     /locus_tag="ACHA6Y_001863"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM8987949.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3515..4438)
                     /locus_tag="ACHA6Y_001864"
     CDS             complement(3515..4438)
                     /locus_tag="ACHA6Y_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM8987950.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5176..6462)
                     /locus_tag="ACHA6Y_001865"
     CDS             complement(5176..6462)
                     /locus_tag="ACHA6Y_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8987951.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6455..7723)
                     /locus_tag="ACHA6Y_001866"
     CDS             complement(6455..7723)
                     /locus_tag="ACHA6Y_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8987952.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            7975..8436
                     /gene="mscL"
                     /locus_tag="ACHA6Y_001867"
     CDS             7975..8436
                     /gene="mscL"
                     /locus_tag="ACHA6Y_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM8987953.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8495..8941)
                     /locus_tag="ACHA6Y_001868"
     CDS             complement(8495..8941)
                     /locus_tag="ACHA6Y_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8987954.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         2482 a         1956 c         1606 g         2973 t
ORIGIN      
        1 aactcacgat ttctttattc aataaaaaaa cggcaggtca aggagagtac ctactcttca
       61 gaccagccgt ttttgtttat cataattttt attcttctac taaaggcaat tcttcttgtc
      121 ctttttcatc ttctgggaca gcgacttctt caccaatacc ataagctgct ctaactagag
      181 cagaaacttc tgccatcatt tcaggatgat tagccatata gattttagcg ttttcccgtc
      241 cttgaccaat ccgatcttct ttataagaat accaagcacc gcttttatca acaatgtctt
      301 tttctactgc catgtctaga agttctcctt cttgtgagat ccctaaacca tacatgacat
      361 ctacttcagc gattttgaaa ggaggtgcta ctttgttttt tacgactttg atctttgtac
      421 ggttaccaac aatatctgta ccttgtttca attgttctgc acgacgtact tccaaacgga
      481 tcgttgcgta gaatttcaat gcgcgtcctc caggagtgat ttctggattc ccaaacatca
      541 cgcctacttt ttcacggatt tggttgatga aaatagcgat tgtttttgtt ttgttgatcg
      601 aaccagagag tttacgcaat gcttgagaca tcaaacgtgc ttgtaacccg acatgtgagt
      661 cacccatttc gccgtcgatt tctgctcgtg gaactaaagc agcaactgaa tcgactacta
      721 cgatatctac agccccactt gatactaaag catcagcgat ctctagacct tgttctcctg
      781 tgtcaggctg tgaaagaagt agttcatcga tattcacacc taatttttgt gcatattgcg
      841 gatctaacgc atgctcagca tcaatgaaag cggccgttcc gccatttttt tgtacttctg
      901 caatagcgtg tagtgcaact gttgttttac ctgaactttc aggtccatat acttcgatga
      961 tacgtccacg ggggtaaccg ccgacaccta aagcgacatc taacgcaaga gagccacttg
     1021 gaattgtaga gatttgttgg tcgatttttt cgcccaattt cataatagag cctttaccat
     1081 agctcttttc aatttttttc agtgcagcat ctaaggctgc tttacgatca tctgccaata
     1141 cgataatcct ccttatattt acaaatcaga gcaacatttc attatcctta tcatatcttt
     1201 tttagtgaaa aaaagcaaga aaaaaccgaa caaatattcg catttatcat tttcttaaaa
     1261 gctgttttct caaaagatca aatccttgca taaccgcatt atatctgata gcagcacgat
     1321 ctctggcaaa gtgatatttt tccgaaacca cgccgtcttt agatgaaata gcgatccata
     1381 ctgttcctgc agattgacct tctaacgtgt ccggtccagc cactccggtg aaagctacac
     1441 tgtaatctgt tcttgctatc tcacgtgctt gaatggccat ctgctccaca cattctttgc
     1501 tgactgttcc atattctgct aatagctccg gatcaatctc cagaaaacca gcttttgttt
     1561 ggagcgagta ggtaacgaat ccgcctggaa atacttcaga tacaccagca atgtcgccta
     1621 atgcggcttg gaaggcgccg gctgtcaaac tttccgctgc tgttacagtc tgcttgtttt
     1681 ctttcagtag ttccactacg acttttgcta gcgaattatc atcgccgtat ccataaaaat
     1741 attcccctac tcgttcttgg attttttctt ctaatgctaa aagagcttga ttccctgcct
     1801 gaacatcatt tgttttcact gtcagtcgta atgtcacttc atttggtttg gcatatggtg
     1861 cgatcgttgg attgatctgt gtctcaatca gatcttttag ctcagtgacc agtcgcgatt
     1921 caccaatccc ataaaagcgc aaaactttag aaattagttt ttcttcagaa ggaaaacgtt
     1981 cctctagtaa aggacgtact tgttccacaa acattggttt caactcatta ggcggaccgg
     2041 gaagaaggat atacgcatgt gtatctgttt gataaaaagt ccctaaagca agccctgtac
     2101 gatttggtag aggaaccccg ccttcgataa tctgggactg ttgcagattg tttttggtca
     2161 tttttcgatg ggttgtttca aaatatgcca ataatttttt gtatccttcc gtattttgta
     2221 tgagagactt accgaggtga gccgcagttg cttcttttgt caggtcatct tctgtcggtc
     2281 ctaatccgcc acataagagt atgagttcac ttcttgtttc agctagagca atcaactctt
     2341 ctaaacgctg ctgattatct cctacaactg tttgataata aacttcgata cctagatcag
     2401 ctagctgttc tgacaagaaa gtggcattcg tattcacgac ttgtcctagt aaaagttctg
     2461 ttccgacagc tattatttct gctttcatta cttcccgctc ccttatatag tatacgctgt
     2521 atttttatat ttttttattt aaaaagaggg caaggagctg tgacaaaaat cttgtcacag
     2581 ctccttgttc caaataaaca gtgttctcct gccaaaacct cggtcataag tcacaatata
     2641 cataaaacat gaaaagctgt tttttgaaat tatcccttgt gactcagagt tagacggcag
     2701 gctcacaacc tctccactaa acgatactta cattgatcct ttgaacacac cgctgttttt
     2761 gatgaagtaa tccacacctg aatagatagt aaagatcaga caagcataaa gcatgatttg
     2821 atctactgga ataccgatca ggctgaatgg gatattattg ataaacagca agataatagc
     2881 aaccatttgt gtagccgtct tgacttttcc cggccaagct gcagccatga cttctccatg
     2941 ttcgacaagt agtaaacgca agcctgtgac ggctaattca cgacagacaa tgatcgctac
     3001 tacccaacca ggggcctttc cttggtcaac caacatgata aaagcagtca taactagcat
     3061 tttatctgcc aacgggtccg caaacttgcc aaaattagtg acaaggcctc gcgcacgggc
     3121 aatttttcca tctaaccagt ctgtgatact agctaaagca aaaataactg cacctactag
     3181 ctgtgtgaca gccagtgtgg tgtctcctac gttaaattgt ccccaatcta aaggtaaacc
     3241 tacaaccaaa atgaaaatcg ggatcataca aattctaagt acagttaatt tatttggtaa
     3301 attcacacta tcgactcctt tcttccctat tctacgtgac tatcagcgaa gtttccaccg
     3361 atattattta cttttccaaa tgaatttatt caaaggagta tttttattga aattgatctt
     3421 tttgaattaa cgaagaagga ccgcgacaca gatttgtcac gatccttctt ttttatacct
     3481 acaagtaaac agatgcgtaa actaatttat acgatcattc tgcgtattga agagtaaggt
     3541 tcacattttt tggactctga ttgttttccc ctggattaac tggtacggat tctccattcg
     3601 ctttgatcgt tgcattgctt gctgcaccaa tcgtgatggt cgcatttgtt gctgtttccg
     3661 gtaacacggt actctgtgtt tcatttgctg ccagtgtccc ttgataaaca taggcattgt
     3721 ctactagcac acctaaccat acacgattga cagcagtaaa ttcaagggtt acaggcttgg
     3781 tagcgtctgt gatatttact gcgatggcac tgccagaatc ttctcctgta gctattgcca
     3841 ttttcttttc ttcacttgat tttgttgttg attctactgt agaagactct ttggtctgcg
     3901 tgctttgctc tgaggaagag gactcctcga ctgatccttc tactttaagc gaagaagctg
     3961 tgtctccgat tattggatca gcttgacgat cttgccaagt catatagccc acgacaacga
     4021 caatcgccaa agcaaccaat cctaataaaa tgacaggaag acttgtccaa aagcgacttg
     4081 gatgcgattc ttcctgatgc attgcttttc tagaagtact tactgtttca ggctgcggac
     4141 gttcagggac cgatcctgtc aatttgcttt tcccatcaaa aacatcgact aaatggtctc
     4201 catcttctcc tacagcttca gcgtattgcc gaataaatgc ccgcacgtaa aatttaccag
     4261 gcaattggtc aaaatcatca ttgtcgattg cttccaaata acgtttttga atttttgtaa
     4321 tctgctgtaa ttcatctaaa gatatttttt tattcaggcg tgcttgccgt aatttttctc
     4381 cgatggtttc aaaagccact cgttcatctt ctccagtctg tttgattctc cgaagcatct
     4441 gctgttgcaa cgaaagacgt tatgtttggg tgctgtgaca aaagtcatgt cacagctcct
     4501 tttttccgaa taaacattgt tctcctgcca actcctcggt cacaagtcac aatatccaat
     4561 aaatataaga agctgttttt tgaacttgtt ccttgtgact cggagttaaa cggcaggctc
     4621 acgacctctt tcttaaacgg tgttcaatca gctgttcctc ggtattagct caaaaaccgg
     4681 caaaatataa ggagatattt ttccgtatgt ttctctaata ctcaggtcaa aacgctgatt
     4741 tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agtccaacca
     4801 agttaaaaac attaagaagc tgtttttact tggctgttct tattacttga tgcagggcag
     4861 ctttttcaca acctcttggt acacggtgtt ccagaagtag cttcttcggc aataagccga
     4921 aatatcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
     4981 cacttctgtc acaacctctt tgattacttc gtttgatttt attttaatat aagttgatta
     5041 aaagagagca tgatttgttt tattttctca ctaatttttc caacacaact aagaatacca
     5101 taaaagctga ttttggagga ggttcctact acaagtctaa aacgaaattt aagacttttc
     5161 ctcataaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
     5221 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
     5281 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
     5341 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
     5401 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
     5461 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
     5521 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
     5581 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
     5641 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
     5701 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
     5761 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
     5821 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
     5881 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
     5941 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
     6001 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
     6061 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
     6121 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
     6181 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
     6241 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
     6301 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
     6361 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
     6421 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
     6481 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
     6541 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
     6601 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
     6661 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
     6721 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
     6781 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
     6841 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
     6901 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
     6961 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
     7021 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
     7081 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
     7141 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
     7201 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
     7261 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
     7321 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
     7381 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
     7441 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
     7501 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
     7561 gataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
     7621 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
     7681 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
     7741 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
     7801 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
     7861 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
     7921 ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc attcatgatc
     7981 aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt
     8041 attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat cacaccacta
     8101 ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc actttcaatt
     8161 cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc
     8221 attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg
     8281 aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac tgcagaagat
     8341 tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa tacacatcca
     8401 gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa aaaagaaagc
     8461 cgattttcag tgtcggcttt ctttttttag attcttaact tgctacttgt tcctttttca
     8521 tgtcgatatg tggaatatca tcttccaaat acacatctga gattgccttg aatccaaacg
     8581 actcataaaa agcttgtagg tattcttgtg cgccgatcac gattggtttt tctggaaaat
     8641 ttttctggat catgttaatc gtttcttgaa caatctttct tcctaaacca tctttacggt
     8701 tgtcctgatg aacaaggaca cgtccaaaat ggatactctc cgcttcttca tatactcgcg
     8761 tgtaggcaag gatcttccca tcagtatctt gaaaaaacgt atgccaagct tgtccatcaa
     8821 tgtcatcaac ttcttgataa ggacactgtt gttccacaac aaaaactgcg attcgttctt
     8881 tgacgatcgc gtaaaactct gctgttgaca attcttcaaa tttctttacc tgatattcca
     8941 ctaagcaaac gccccctgct tttttattct tagcataccg attcgcacac aaaaaagcaa
     9001 acgaacctta cgaatct
//
LOCUS       DAYYHX010000109         8965 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000109 DAYYHX010000000
VERSION     DAYYHX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.110"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACHA6Y_001869"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACHA6Y_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM8987955.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACHA6Y_001870"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACHA6Y_001870"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM8987956.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ACHA6Y_001871"
     CDS             complement(2169..2906)
                     /locus_tag="ACHA6Y_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8987957.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ACHA6Y_001872"
     CDS             complement(2997..3524)
                     /locus_tag="ACHA6Y_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987958.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ACHA6Y_001873"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ACHA6Y_001873"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM8987959.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ACHA6Y_001874"
     CDS             complement(4401..4886)
                     /locus_tag="ACHA6Y_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM8987960.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="ACHA6Y_001875"
     CDS             complement(5039..5236)
                     /locus_tag="ACHA6Y_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987961.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACHA6Y_001876"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACHA6Y_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM8987962.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACHA6Y_001877"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACHA6Y_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM8987963.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
BASE COUNT         2533 a         1987 c         1500 g         2945 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttg
//
LOCUS       DAYYHX010000110         8940 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000110 DAYYHX010000000
VERSION     DAYYHX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.111"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..474
                     /locus_tag="ACHA6Y_001878"
     CDS             10..474
                     /locus_tag="ACHA6Y_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8987964.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            458..1087
                     /locus_tag="ACHA6Y_001879"
     CDS             458..1087
                     /locus_tag="ACHA6Y_001879"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8987965.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1441..2919
                     /gene="msr(C)"
                     /locus_tag="ACHA6Y_001880"
     CDS             1441..2919
                     /gene="msr(C)"
                     /locus_tag="ACHA6Y_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8987966.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2966..3412
                     /locus_tag="ACHA6Y_001881"
     CDS             2966..3412
                     /locus_tag="ACHA6Y_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8987967.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3611..4126)
                     /locus_tag="ACHA6Y_001882"
     CDS             complement(3611..4126)
                     /locus_tag="ACHA6Y_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8987968.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4243..5445
                     /locus_tag="ACHA6Y_001883"
     CDS             4243..5445
                     /locus_tag="ACHA6Y_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8987969.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            5818..6144
                     /locus_tag="ACHA6Y_001884"
     CDS             5818..6144
                     /locus_tag="ACHA6Y_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987970.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(6261..6605)
                     /locus_tag="ACHA6Y_001885"
     CDS             complement(6261..6605)
                     /locus_tag="ACHA6Y_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987971.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(6681..7928)
                     /locus_tag="ACHA6Y_001886"
     CDS             complement(6681..7928)
                     /locus_tag="ACHA6Y_001886"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM8987972.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(7925..8731)
                     /gene="proB"
                     /locus_tag="ACHA6Y_001887"
     CDS             complement(7925..8731)
                     /gene="proB"
                     /locus_tag="ACHA6Y_001887"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM8987973.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
BASE COUNT         2720 a         1601 c         1869 g         2750 t
ORIGIN      
        1 gaggaaatta tgtctttaga agagattagt caccttttat atcaaatcaa attagccgat
       61 caaaaaatcg tccagatctt tgaagaaaat atcggtataa gtcttactag atacgagatc
      121 atgatgagac tgaaagaaga aaatccattg ctacaaagca gtttgcaaga aaagttgcag
      181 attgatcaag gagcagttac tcgtcatctg aaggtattgg aagcaaatgg ctatgtttcg
      241 cgtcagagaa atcctcagaa caaccgggaa gtgtatgtag cgctaacaga aaaagcccag
      301 aagaaaatcg agcattgtga aatggaccat aaagatacag gttccgtgct atccccggct
      361 ttcagcgatg tagaaatgaa acaatttcta acacttttag ataaattgaa tacccaactt
      421 tgcaatataa caacagaaac gaaagaggtt aataataatg gaacaaaaaa atgatttttc
      481 aacaattatg actggtagaa gatccgttcg cgtatatgat gaaacataca aaatcccaca
      541 cgaagaaatg ctggaaatga tcaaagaagc gacgactgca ccttcttctg tcaatatgca
      601 accatggcgc tttgtggtcg ctgaaagcga ggaagcaaaa gaaatcttgc gtcctttgat
      661 ccgtttcaat acgagacaaa atgatacttc ttctgcaatg gtcatgattt ttggcgatat
      721 gcaatgttac gaatatggcg aagaaatcta taaccaagct tacgaatcag ggaagatgcc
      781 aaaagaagtc agagatcaac aattagctgc gatcattcct tattacaaga gtttttcaag
      841 agaagaaatg aacgatgtag tcaaagtcga ttctagctta gcggctatgc aattcatgtt
      901 agttgcgcgt gatcatggct atgaaacaaa tccaattggt ggagtcgaag cagatcagtt
      961 ggcagaggca tttggtttgg ataaagaccg ttatgttcca gttattattt tatcaatagg
     1021 aaaagcggta gaagaaggtt acgagtctgt tcgtttagca ccagaaaaga tcactacgtt
     1081 cgaataaaag gttttcagtt actattcagt agctattgct ttttttgaat cgtgtgtggt
     1141 aacctaataa aaaaggaggt cggaaaaatg actgcatcga tgaaattacg tttcgaactt
     1201 ttgaataaca actaaataaa ttcaaaagac atgtataaac aacaatttca tggatggagt
     1261 atgtccattt tttcgaacga cagtagaata ggtgaaagct atgacagatt tggtgattta
     1321 gtcatagtta tttcgttcag agtaggaaaa gatgtgatgc agtttttttg caaacagatt
     1381 tttctgctct tttttagtat cgataattgt tgttttacat gaaataggag tgtaaaacat
     1441 atggaaaatt tagcagtaaa tataacaaat ctacaagtca gctttggcaa ccagctagaa
     1501 ctatctattg attctcttcg tgtctatcag caagatcgta tagggatcat aggagaaaat
     1561 ggtgtcggta aatcgacttt gctcaaacta atagccggtg aactttttcc cgatcatggg
     1621 aaaatccaaa cagagatcac cttcaactac ctgcctcaat taacctatct cgctgaagca
     1681 aaggacctaa atttggaatt agccagtcat ttccagttaa gactggaaga aacttcagag
     1741 cggaaatgga gcggagggga agaacgaaag atcgagttga tacgtcttct ttcttcttat
     1801 gaacaaggga tgcttctaga cgagccaaca acccatctag atagaaaaag tattgatcga
     1861 ctgattgaag agcttcgtta ttattatggt acgctggttt ttgttagtca tgatcgctat
     1921 tttctagatg agctagcatc gaaaatctgg gaagtaaaag acggagaaat ccgagagttt
     1981 tcggggaatt atagtgccta tctcactcaa aaggaattgg agaaaaagac tcagctacga
     2041 gaagcagagt cgatcatgaa agagaaaaaa cgattggaaa aatcgatcca agaaaagaaa
     2101 aaacaagcgg aaaagttgga aaaagtgtcc agtaaaaaga aaaagcaaca aatcagaccg
     2161 gatcggttgt cttcctctaa acaaaaagac agtgtacaaa aagccatcca aaagaatgcg
     2221 aaaacattag agagaagact ccaaaaaata ggagaaacaa ccaagccgca gcagatgaaa
     2281 caaatccgtt ttccagtacc aaaatctctt gaactccaca gccgctatcc catcatggga
     2341 caaaatgtcc aattggaaag aagtgggaga acattactgg taaatggtga ttttcagttt
     2401 tctttaggta aaaaaatcgc gattgtcggt gaaaatggtt caggtaagac aaccttatta
     2461 gaacatatcc gcaaacaagg agaaggaatc cttctctctc cgaaagtaag ctttcaagta
     2521 tatcagcaaa agggttatca aatgacatct gaagaatcca tcattcgttt tgtcatgaga
     2581 caaacagagt tttcagaatc gcttgtccgt agtttgctga atcacttagg gtttgctcag
     2641 gaaactttga cgaaaccgtt atgtacatta agtgggggag aagcgacccg tctgacgatt
     2701 gctttgcttt ttactaagcc aagtaatgtg ttgctgttag atgaaccgac taattttatt
     2761 gatatggcaa cgatcgaagc tttagagaag ctaatgcaaa tatatccggg aacgattttg
     2821 tttacttctc atgattctta ctttgtcgag cgtacggctg atgaagttta tgaaataaaa
     2881 gggcagaaaa taaaaaaagt acttacgaga aatttttaaa tgtgctacga tgattttcgt
     2941 gacaaattta aaggagtggt acagattgcc tacaaataaa aaagaaggaa taatttttac
     3001 aacaatgatg tgttttttga tggtcttggg gatgagcgcg tataatttat ggctgcatca
     3061 agatcttcat ttatcaagtt tgttgattgg tctagtgcct ggattcgtgg tggctttcat
     3121 tttggatgtc ttcatcatcg gtgtgctggc gaaaaaaatt gcctttaaac taccagtaaa
     3181 tccagaaagc aaggtccaaa tgatcttaac tatttcatgc ttgatggttt taggaatggt
     3241 gacatgtatg tctttattcg gtgtgatcat ggaacaagga atacctgatc aattattctc
     3301 tgcttatatg aatgcttgga aaatgaatgt gattgccgct ttaccgttgc agttgatcat
     3361 cgttggcccg ctttcaagaa gtgtgctaaa agcaattcaa agtaatgaat gatggaacac
     3421 tgttatttga aaaaaggcct tggcatgctt tttgtcatag tctcttgaat gcagttctag
     3481 aaaaatagtg atccgaacga taggttttgg cctgtcgttc ggatcgcttt ttcttttcca
     3541 actgtattgc tcttttattg aagataattt gctcttcaat agatatttct agaaaggttc
     3601 tgtttatccc ttactcaaaa aactgctgca tttgttctct tacttgctga caccattgga
     3661 gttcctgctg acaaagcata attctgcgct ggatcacaac ataacgtcca aattcttgtt
     3721 tcttgtctag cgagaggtca ttcaattttt tttgattttt ttgcatgatt ttgttcagct
     3781 gttgttctct agtgaatagc agtccagtag ctgttgtttt atcgagcgtc gaaaaagcat
     3841 agatttttgc tagaaattca tctttttgcg taggagcggg agtttcctct ttgatccatt
     3901 cctctacaag agcacgtcct tcatcagtca agtggtagat ctttttttga gagtgctcgt
     3961 ctttgtccaa aacggtcaca taaccctgct ggtctaactt ggcaagcgtt gtatagatct
     4021 gactgtgatg ggcttcccaa aaaattgtga tcagctgctt caattcatac ccagaacgtg
     4081 gtttggataa aagcacgcat aataatatat aggctaaagt attcataaaa atcctacctc
     4141 tcgtcttatt ttctttatta tatcacagta aagtttccac gttttacatg tgcatattta
     4201 catgtaaaat aaatcatgta tgatgctaat agagagaaag ggatggaaat attggagaat
     4261 caaatagata tacaagaaca aacaaatgaa gaaacaaagg ggaaaagttc agcgctagtt
     4321 gtagcagcta cctgtatgat cgcttttatg ggagtgggtc tagtagaccc aatcttaaaa
     4381 acgattgctg cacagttaaa cgctacgcca gctgaaacga ccctgttatt tactagttac
     4441 atgttagtca ctggtgtggt gatgctattt acaggtttct tatctagtcg gatcggtttg
     4501 aaaaaaacat tgatgacagg attgtttatc atcgttgtat tcgctggtct tggcggatta
     4561 tctgggaata tcggaatgct gattgggtta cgtgccggct ggggtgtcgg gaatgcttta
     4621 tttgtctcta ctgctttagc aacgatcgtc agtattttag ttggtaacac agaaaaagca
     4681 atcatgatgt atgaagcagc acttggctgc ggaatggctg ttggccctct agtcggtggg
     4741 gtcttgggca gtggttcatg gcgttatcca ttttttgggg tagcattttt gatgttttgc
     4801 ggttttatag ccctagcggt acttttacct gaaactgaga aaccaaagag aaaagtaggc
     4861 ctttgggaag gtgtcaaagc gttaagcaat cataaacttc gttcagttgg actaatcgca
     4921 ttgttatata atttcggatt ctttacttta cttgcgtatg cgccattttt attgattggc
     4981 tattctgaat tagaagtcgg ttttgtattt tttggctggg gtgtgcttct ggctattgct
     5041 tcgattttcg ttgcaccggc tcttgagcgg aagtttacga cttatcggac aattttagca
     5101 gcatttgtct tattcattat ttgtttgctg ttattaggag ctggaacttc tatgccagtc
     5161 cttgtaccga tcagcattgt gttagcagga tttttccaag gaattatcaa tacactgttg
     5221 acgacgattg ctatggaaat accaggtctg caacgaaacg ttgcttcgtc cagctatagt
     5281 tttgtccgct ttttcggtgg agctttagca ccatttattg caggtgtgat tggtgaacat
     5341 attggtgaaa gctatgcgtt ttattttgca ggtgccattg ttcttatcag tgtatgttgt
     5401 atcatttatc accgtaatta ttttgctaca aatgaagaag aataagaaat cagaggttgt
     5461 gaaaaagctg tcctgcatca agtcataaga acagccaaac aaaaatagct tctcatattt
     5521 ttcgtttggt tgggcttatt accgcagatg caagcttttg aacgccgttt accaagaggt
     5581 tgtgagactg tcgtttaact ctgaatcaca aggaacaatt tcaaaaaaca gtttctcatg
     5641 tttgttgaat attgtgactt atgattgagg agttggcaga agaacaccat ttattcggaa
     5701 aaaggggatg tgacacgatt tttgtcacat tccctttttt tctcaaaata atgaaactgg
     5761 aaaattttca gtaggtaagc taaaattcat ttaagaaaaa gaatgggggg ataatagatg
     5821 ctggaagaat attttcagga agtcgggttg acactcgttg aattacccat tgaattttca
     5881 gatgatcggt tgatttatcg tggctatatc aaagaattgc caataatcaa tgcggaaggt
     5941 gaaactagaa aagcgatgca gcaaaaaata gctggtatgt atcaggtgta tcgggaacag
     6001 ctactggctg aatcagcgga agaagaaaaa gaaaaaacga tgaacttgtc catggatgag
     6061 ctgcttcgtt attatgatgg cgaaacattt gacggatttt cgcttcttaa cgaagaggaa
     6121 acagatttac caggcagctg gtaggcgaac gccgtttata tggaaaaaga ggctgtgaca
     6181 aaatctttgt cacagcctct aaaaagtccg gcagtttttt gccgatcttc aaggcactct
     6241 acttatcgta cggatatttt ttagcggaaa gtccgattca agtggttttt attcagctga
     6301 tatttataag ataagtgaaa aatcccagca atcagtagcg taatcgtata agcgattgaa
     6361 tgatcgaaaa aagaatagta attgattgtc gctaataagc ataatgcaac catgtaacta
     6421 atgtgcaagc gtgtaaccag ccctttttgc ccgattttgt caaaatactg cgaccatttt
     6481 tcccgttgac tccctatgct tcgttttttg aattgaaaga aaagggataa aataccgact
     6541 cccagaaaag cagcgatatt cagagaaata aggtaaataa aaaaaatcat taaaatttca
     6601 aacatattga cacctttcta gttggataga acctttacag aaaaaaggca aaaggcggga
     6661 ataatgtttc acacgaaaca ttattcgcgg atttgtccat cgccataaat gatatatttt
     6721 gtggaagtta attcatttaa ccccattgga ccacgggcat gcaacttttg agtagagata
     6781 ccaatctctg caccaaaacc aaataccgat ccgtctgtaa aacgagtgga ggcattgaca
     6841 tagacagccg ccgcgtccac ttcatttaag aaacgttgac tagccgcata actatctgtc
     6901 acgatcgttt ctgaatgttt agtattatgc cgattgatat gtgcaattgc ttcatccaaa
     6961 gaatcgacca ctttgacagc aaggatataa tctagatatt cagtatccca atctgtgtct
     7021 tctgctaaag cagccgtctc tagatattct aacgcacgag catccgcacg taaagagacc
     7081 ccgtgttcta ccagttcttt ttcaatagct ggcaagaaaa aaggtgcgat ctcagaatgg
     7141 atcagcaatg tttctgccgc gttacagaca gatggacggg aagcttttgc attgacgatg
     7201 atgttcactg ccatagataa ctgtgcttct ttatcgatat atacgtgatt atttcctgta
     7261 cctgtttcaa tgataggtac cgttgcctgt tctttgactc gttggattaa ttttgctccg
     7321 cctcgaggaa tcagcacatc taagtattct gtcaatcgca tcaactgtcc tgcagtttca
     7381 tgagaagtat catcgacaaa ctggatggca taaggagatt cgccagcact gaccagtact
     7441 tgctgcaata attggactaa taattgattc gagaagaatg cttctttgcc accgcgaagg
     7501 ataacggcat tccctgattt gaatgctaga ctagctgcat ctgtcgtgac atttggacga
     7561 gattcgtaaa tgatgccggt cacgcctagt ggcacgcgct ttttgccgat tctcaatccg
     7621 tcggtgttcg tccacatctc atctactttt ccaataggat cgtctaacga agcaattttt
     7681 tcgatctctg tagccatggc attgattcgc tcaggtgtca gctgcaagcg gtcttgcatc
     7741 gtttcactga tattgttttc gactgctttt tctaagtctt tatgatttgc ttcaatgatc
     7801 gcttgttgat tctttttgat ggtttgtgcc atttgaacaa gtagctcatt tttgcggttt
     7861 gtaggcataa gtgctagttg ttgcgcgcta tcttttgcct gttgtcctaa ttgcatcaag
     7921 tcagtcattt caagtcctcc ttaaataaag tacctacttc ttttcctgca agaatatcaa
     7981 aaataattgc cggatttttt ccatttgcta aaatcattgc gctgtttgct tcaaatatcc
     8041 tagaagcggc ttttaattta ctctgcatac cgcctgttcc aaatgtgcta ccagcaccac
     8101 ctgcttgtgc cagaatcttt tcatcgattt tatcgatata gcgatacatc tgagcctcgg
     8161 gatttgtagt cggattatct gaataaaacc cgtcgatatc agaaaggacg atcaaaaggt
     8221 ctgccgcgac aagttttgcc atgatggctg acagctgatc attatcgcca aattttgtca
     8281 gatgatctaa ttcatccact gctacagtat cgttttcgtt cacgataggg atcgtgtcca
     8341 taatcaaaag ctgttccaat gtattcctca catttttacg actttcagga taatctacca
     8401 cgtctcttgt cacgaggatc tgtgccgtct gttgattata tacactgaat ctttgattat
     8461 agatattcat cagttcggct tgaccaacag ccgccgcagc ttgctgttca ggaatcaaag
     8521 tcggacgttc tgacaaatgt aatttatgca taccaacgcc tacagctcct gatgagacta
     8581 agatcacttc ctttccccga tttctaagat cagaaaggac aaaagctaac tgatcgattg
     8641 ccgcaagatt gatatttcca ttgggataaa tcaatgtact tgttcctact ttgacaataa
     8701 ttcgttttgc atttttaatt tctttacgca tgtgacacct caatcaaata tccactcatt
     8761 aatttcgttc tattctactt gttttgacga gtaaagtcat gtaaaaaata gtttttttgt
     8821 acaacttgta gtcgtttgga aaaagaacga attaatggtt tactttaaga tagcagaatt
     8881 tcttgaagaa tttgtaaaga aagtctttta cagcctcgga tatgttagaa taggagtagt
//
LOCUS       DAYYHX010000111         8862 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000111 DAYYHX010000000
VERSION     DAYYHX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.112"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..917)
                     /locus_tag="ACHA6Y_001888"
     CDS             complement(<1..917)
                     /locus_tag="ACHA6Y_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8987974.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQK"
     gene            1104..1595
                     /locus_tag="ACHA6Y_001889"
     CDS             1104..1595
                     /locus_tag="ACHA6Y_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM8987975.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(1619..3994)
                     /locus_tag="ACHA6Y_001890"
     CDS             complement(1619..3994)
                     /locus_tag="ACHA6Y_001890"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM8987976.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            4320..4946
                     /locus_tag="ACHA6Y_001891"
     CDS             4320..4946
                     /locus_tag="ACHA6Y_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8987977.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            4969..5673
                     /locus_tag="ACHA6Y_001892"
     CDS             4969..5673
                     /locus_tag="ACHA6Y_001892"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8987978.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            5865..6698
                     /locus_tag="ACHA6Y_001893"
     CDS             5865..6698
                     /locus_tag="ACHA6Y_001893"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM8987979.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            6895..8823
                     /locus_tag="ACHA6Y_001894"
     CDS             6895..8823
                     /locus_tag="ACHA6Y_001894"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8987980.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         2708 a         1693 c         1636 g         2825 t
ORIGIN      
        1 tttttttgtg aacgattttt tatttctggc aatacgccag ttgttcgata cattttttgt
       61 tcgttttcat ttgcaaaccg gccaaatact gaatgagttg gtaaaatatt gataaatgca
      121 tcgtcgtcga tttcatgaac gatctgatca agatcataca tttcgtagcg ggtgatgacc
      181 atcatgatca tgttcccttg tttttttgaa tagccaccga cagacggcag taatgtcatt
      241 ccacgtacca tccgttttgc tagttcttct gttacctctt ctggcttgac ggtaacgatc
      301 attgctgtta ctttttgatg actcgtgtgg atggcatcaa ttacttgagt cattgcataa
      361 atggaaataa tcgtatacaa ggcactttcc cagttaaaga agaagcctgc gatgactaca
      421 atgataccat ttagaatcag catgtagttt ccgactgttt ttccagttgt ttgtgaaagt
      481 acaagagaga tgatatccat cccaccagtt gtaaagccca tttttaaaga taatcctacg
      541 ccaagaccta ataaaacacc gcctactagg gcattcatca aaatattatc agtgatctgt
      601 ccgactggaa gcagcatcgt catcaccgat acacatacaa cattcacaaa actaaggatc
      661 gttgctcgtt ttcctacttt gagaaaccct aatacaaaca cagggatatt caaaagaaaa
      721 ataaatgttc ctgtgtctat cttgatatga aaccattcat ataaaagcga ggaaatgatt
      781 tgagcgatcc cattcatccc tgcagaaaag acattggctg gaatcaaaaa ataatttaaa
      841 gcgaccgcag cggtcaatcc ggttatcaaa ataaccacta ttttctttaa tctttcattt
      901 ttatgatacg cagtcataat tttactcaca agcacctctc ccctactttg gtattccaaa
      961 tgtaagaata acagtttatc tattaaaata aaatacttcg aactcaaatg agaagaattt
     1021 tagcaaacag gtaagaatct gccctaatcc tatgataaga tagatgtatg gtaaatattt
     1081 atatgtatat caggagggtt cctatggcaa aagatgctag cttcgacatt gtatcagaag
     1141 tcaatacaga agaagtaaaa aatgcgattc agcaaacaac aaaagaattg tccaatcgct
     1201 ttgacttcaa ggattctaca gtagaaatca aattagagaa cagcaatcta gttgtattga
     1261 gcgatgatga tttcaaaatc gaacagatca aagatgtatt atttggtaag ctgatcaaaa
     1321 gaaatgtgcc aatcaaaaat atccattttt ccgaatcaga acacgctcta ggtggcaaag
     1381 ctcgacaaac tgccgatttg gtcaacggaa tcgatcgtga aaacgcgaag aaaatcacta
     1441 cagctatcaa aaatgaaaaa ctgaaagtaa aagcacaaat ccaagaagat cagattagag
     1501 tgacaggtaa gaaccgtgat gatctacaag ctgtgattgc tttattacgt aaacttgact
     1561 taccaatcga gttgcaattc actaactatc gttaatcaaa aaacgaggct gacgtctgtc
     1621 attcctcgtt tttttgattt actgcttctt ttgataacca aaaacgctgt attccttctt
     1681 ccaattcccc aacacttcgt acttctcttg ctgtttgcca atgttgtggg aataaacgtc
     1741 gataagaaaa actagaaaat cgttgatctg ccaatacaac gattccttga tccgccatgc
     1801 cacgaatcac tcgaccacca gcttgcaaga ctttgttcat tccaggaagc tgataagcgt
     1861 aagcaaatcc aaggtgttcc ttctcatcat aatatgattt gatcagttcc tgctcatgat
     1921 tcatctgcgg caaacctaca cctacgatca tactgccgat caatcggtcc cctctcaaat
     1981 cgattccttc tgaaaaaatc ccgccaagta cgcaaaatcc aatcaatgtt tcagcgggat
     2041 ttgtttgaaa ttttgttaaa aatcgttcgc gttcttcctc attcatgtcc gtttcttgga
     2101 ttagcacatt gctgtcggga tattgctgtc tgaatgcttc caccgcaaga tctaaatatt
     2161 gataagaagg aaagaaaacg aaataattcc cgacctttcc atcagcaaaa gcatgaatcg
     2221 tctctactaa ttttgccaga ctgtgttccc tttttcgata agtcgtctct aggtaatttg
     2281 cgatgaaaac ttgctggttt ccttctggga aaggactcgg cagtttgtaa gccagacttt
     2341 tttgtccccc cagtgtttcc tgataataag agagtggtga aaaactagca gaaaaaagaa
     2401 tactgctttt tcctttatcc aacgactgct caagaaacaa gcttggatca atacaaaact
     2461 cttttactat cagatcgcga tacgtttttt cgactgttgt ttcgtagtga tcatcataaa
     2521 attcactgac cttcaaaaaa tgaagcaaat caaaataata aggaagcaat tgttcttgtg
     2581 atgggtgctc aggaaattct gccagccatt ctttaatttt ttcgcttaat tgataacctg
     2641 cctttactaa agattcagcc ggtgcttttt gatgatgata atcccaatga tcttcttttg
     2701 ctatttctcg gatcgaatca aattctttta atagtttgtt gaaacggcga tgaagttttt
     2761 tgaattcctt tggtatagct tccttacttc gtttcgtttt ttcataagat agttctgccg
     2821 aatacatctc tcgggaacga ttcaccaaat tatgtgcttc gtcaatcaaa aacagatagt
     2881 cctcgttttt ttcatcttca aaaaaacgtc ttaaataaac agttggatca aataaataat
     2941 tgtaatcccc aatgattacg tcacaccaga tactgacatc taatgatagt tcgaaaggac
     3001 aaagtgtatg ctttcttgca taagtttcga tgacttcacg agtgatctga ttctcatgat
     3061 tcaaaagatc ccataaacct tcattaatcc gattgtaata accattggca tatgggcact
     3121 gatctggatt acaggtcgtc tcatctaaaa agcagatctt gtctttcgcc gtcaaagtga
     3181 cactttttac ttcagctcct gtatctttta atgctgtcat agcatcttca gctacttgtc
     3241 tagttattgt tttagccgtt agataaaaga tcttttctcc ctcttcttcc cctacagctt
     3301 tcaaagcggg gaataacgta gagattgttt ttcctgttcc ggtaggtgct tctacaaaca
     3361 atttctgttt cgtacggatc gttttataag cagctaccgc tagttcacgc tgacctttcc
     3421 tatactcatc atgggggaat ttcaaagcca tcaaagaggc attgcgtaca gtgcgccagt
     3481 tttcttgaaa aacaagccat tctttataat cttcagtcag ctttttaaaa aaagtttcta
     3541 actgctctct tgtctgatgt tcaacctttc tcgtgatcac ttcttctgcc gtctggaagt
     3601 aagtcaattg cagatttatt tcttgtaaat catgttcttg actatagata taggcataca
     3661 cccttgcttg atgaaagaaa agttccactt gttctggttc taaatcttca aatcgtggtt
     3721 cggaggtttt gatttcatct atataataaa taccgtctct tttgaaaata ccgtctgcac
     3781 ggccttcaac gatcaggtaa tattcatcta attgaactgt tgtttttaaa aagacttctt
     3841 tttcatagtt ttctcccgca tctttttgga gcttgcggtg gattctcgct ccttctaatg
     3901 cagtatggtt acttttttta cgattgtcga tacttccacg tcgcaaaata aattctacta
     3961 agtgccgcac agatattttt gactgtttga tcatttccac cacctctttc tctctaagat
     4021 tacctgaaaa aacctagttt agcaaaaaga gctctctttc caaacataaa aaaacaccct
     4081 gctgattagg cagggatata ggatatagaa tttggatttt tggatgaatc taatgtaaca
     4141 aggacatatt aaaatgctat gaaaacttac tttccttctc tttagaatat ccaaatactt
     4201 ttcatatagt ctgataattc agataagtaa agagaaataa gtacctgttt ttgtttccta
     4261 gcaatgaatg cgttatacta atggatgaaa gaagttttgg ctatcaagga ggaatgcaca
     4321 tgcatatcga aagattaaaa acaggaatga ttgaagaaaa ctgttatttg gtctacaacg
     4381 aagaagcact tttgatcatt gaccctggag aagatgcgga acgaatcaaa caagaaatca
     4441 aaaaaacaaa ccaacagccg gtagcgattt tgcttaccca tactcactac gatcatatcg
     4501 gtgcagtgga acctttacgc gcgtattatc aaatcccagt ttatgtcagc ccactagagc
     4561 aaaaatggct aggtgatccg attttgaatc tttctggact cggtagacat gatgatattg
     4621 ctgatgttat tgtctcgcct gcagaatatg aatttgaaat gaaaacttat actttaggaa
     4681 acatgtcttt tcgcgtcgtc cctaccccag ggcattctat aggaagcgtc agctttattt
     4741 ttgatgattt tgtcgtttct ggcgacgccc tgtttagagg aagtatcggt agaaccgatc
     4801 tgtatacagg gaatttagaa caattacttc atagtattcg gacccagctg tttgtattac
     4861 ctgatgaatt tgcagtctat ccaggacatg gcgatgcaac gacgattgaa caggaaaaaa
     4921 ggacaaatcc ttttttcaat cgttaaaacg acttaggagg agaataaaat gacagaaact
     4981 acactttata tcgtacgaca cggaaaaacg atgttcaata caattgaacg agtacaaggc
     5041 tggtgtgata cacccctcac aaagcaagga caagaaggca ttcattattt aggtcgcggg
     5101 ttaaaaaatg tcgacttttc atttgcttat tctagtgaca gtggtcgtgc aatcgaaact
     5161 gcccgtatta ttctaggaga acattctaag ggaaaagaaa ttccttattt tattgaaaaa
     5221 aggattagag aatggtgttt cgggtctctt gaaggtggat atgacatgga aatgtggggt
     5281 gtgatccctc gtgtattgaa ttttaaaaca tatgatgaaa tgtttaccac agaagtttct
     5341 tttgaacaaa ttgctaatgc tgtatacaac ttagataccg ctgggtgggc ggaaacttat
     5401 gaaacattgc gagaccgtgt atggacagga tttgaagata tcgcccatca ctgcgaaaaa
     5461 aatggtggtg gaaatgtttt agtcgtatct catggtttga cgatcgcctt tttgcttaat
     5521 ctgatcgatc ctaaacagcc tgttcgtgct gggcttttaa atggcagcgt aacaaaggtc
     5581 acttatgcaa acggtcgatt ttctatccac ggcatcaatg atacgtctta tatcgataac
     5641 gggaaaacac atagtcgcga ctcgtttcta taatggggat attttaattt ttaccagaga
     5701 atcataaaag gcaaagtctg aacgagggct ttgtcttctt ttttctcttc gtgtacttat
     5761 ctgctttatt tctcaagttt ttcttaatat taaaaaacag attgggtttc tcaaaaaata
     5821 acggtatact ataagatgta tcttaattgg gaaggaagga atttatgttt tttgcaaata
     5881 ttatcaaagc agtgattctc ggtattattg aaggaataac ggaatggctg cccatcagta
     5941 gtactggtca tttgatccta gcagatgaat tcatcaaact ggatgccagt tcacaattta
     6001 tgtcgatgtt caatgtcgtt atccaattag gggcgatctt ggctgtcgtc gttctttatt
     6061 ttcacaagtt aaacccattt tctccaagta aatcaacggt agaaaaaaaa gatacgtgga
     6121 cactttggtt taaagtgatc gttgcttgca taccagccat catcattggt ttgcctttgg
     6181 atgattggtt agaagcaaat ttccacaagt tcttaccagt tgccattgtt ttgatcgttt
     6241 acggtattgc ctttattatt gtcgaaaaac gcaacaaaaa caaaacacct cgttggactt
     6301 ctctgaatga ctttacgtac caagcagctc ttcttgttgg gttattccaa gtcttggcac
     6361 ttgttcccgg aacatcccgt tctggcgcaa cgatcctagg atctatcttg attggtgctt
     6421 cgcgttttgt cgctacagag ttttcatttt tcttagggat tcctgtcatg tttggcgcaa
     6481 gcggcttgaa gatcgtcaaa tatttagctg aagggaattc attcaaaatg gaagaaactg
     6541 ttgttcttct tgtaggaaca ttcgtatctt ttgtggtttc cgtcttagct atcaaattct
     6601 tactaagcta tttgaagaaa aacgacttta ccgcttttgg ctggtatcgt attatccttg
     6661 gtatcatttt gattggttat tggtttattg caatgtagaa aaaaccagaa cacttctctt
     6721 atttgagaaa tgttctggtt ttttattttc tttaataata atcattataa ataataaaaa
     6781 caacatcaag cttaatcacc aaatgataac cattatcgtt ctcatttagc tgttattatt
     6841 atcaattaca tttttattat gctatccttg ttttgacaag gaggtctaat aacgatgact
     6901 aatcagaaaa aagcttttgc agcaacaatc ggctgccttt tatttatgat cttcggtttt
     6961 atcttcgacc gattctctct taccatcacc ccactttttt atatcagtgc cattatcatc
     7021 ggtggatata aacaaacatc ggaaggcttg aaagaattgt ggaatgatcg gacactgaat
     7081 gtggatttat tgatggcttt agctgctatt ggtgcctgtt tgatcggtaa ttactttgaa
     7141 ggagcaatgt tgacattcat cttttgttta agcggtgcat tagaggaata cacaactaat
     7201 aaaagtcaaa aagaaattac tgctttgatg aatcttcaac cacaaaaagc ccaattatta
     7261 aaagaaaatg gacaaatgca agaagtagat gtttctcaac taaaggtgga taacttggta
     7321 tttgtcgcta aaggagcagc tgttccgata gatggctttt tagaaagcac acaggcaacg
     7381 atcgatgagt ctgcattaag cggtgaatct gtgcctgtag aaaaatattt aggcgatgag
     7441 atgtatgcag gaacgctgaa tcaaggaaat cccatcacga ttcgagtaac aaaaacgagt
     7501 tccgaaactg tctttgctaa gattatccaa ttagtagaag aagcgcagaa tacgccaact
     7561 caaacagctt catttatcga acgaatcgaa aataattatg taaaattgat tttgcttgct
     7621 gttcctctaa tgatccttct cccccacttt ttcctaggtt ggagttggga tgaaagcttt
     7681 tatcgaggaa tggtattatt agttgtggct tctccttgtg cgcttgtcgc atctgctaca
     7741 ccagcttctc tagctgctct ttcaaattct gcaaaaaatg ggattttaat caaaggcggt
     7801 atccatttag aaaagctttc tgaattaaaa gcgattgcct ttgataaaac aggaacgctc
     7861 actaaaggaa aaccagttgt aactgacagc tttttctttg atgaaaagga tttggcacaa
     7921 caacttcttg ttgctatgga acaaaaaacg acgcatccac ttgctcatgc gattttgcaa
     7981 tatttcgatt gcacaatacc tgaagaaatc caatacttac caatagaaga aattactggt
     8041 tttggattac aaactaccta tttaggtgcg caatggaaag ttggcaagca cgcatatgat
     8101 cctgagacga tgataatcag caaagagatt gcggaaatga tcgaacgttt ggaaaatcaa
     8161 ggcaaaaccg tgatttatct ttctaaagat cagcagttaa ttgctgtttt aggtctgcta
     8221 gatattccaa aagcaaatac ccaacaagta atttcctact ttaaatccca aaacatccat
     8281 acaagtatga taaccggcga ccattctggc acagcaaagg ccattgctga acaagtaggg
     8341 attgaacatt actatgcaag ctgtactccg gaagaaaaaa cacaactagt caaaaaagag
     8401 aatgaacttt accaagtaaa tgcgatggtt ggtgatggag tcaatgatgc gcctgcttta
     8461 gcagccgctt ctttaggtgt cgctatgggg caagggacag atgccgctat ggatatcgca
     8521 gacatcgtct taatgaaaag cgacttaaat aaatttgtct acagtcatcg gctatcttta
     8581 aaaatgcggc ggatcatcaa acaaaatatt attttttcga ttcttgttat tctcttgttg
     8641 attgcttcta acttcctgca atttttaaat ctacctcttg gagttgtagg acacgaagga
     8701 agcacaatat tggttattct gaatggcttg cgtttactaa gacctttacc tgatctttct
     8761 gattcttcaa aaaaatgtaa gtcttgtcca ctatacaaag cgcggctgtc ttctgtcaaa
     8821 taagcaaagt gtttttttat gattttgttt attttatgaa aa
//
LOCUS       DAYYHX010000112         8818 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000112 DAYYHX010000000
VERSION     DAYYHX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.113"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..765)
                     /locus_tag="ACHA6Y_001895"
     CDS             complement(<1..765)
                     /locus_tag="ACHA6Y_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HFM8987981.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDY"
     gene            complement(771..1877)
                     /locus_tag="ACHA6Y_001896"
     CDS             complement(771..1877)
                     /locus_tag="ACHA6Y_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8987982.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            complement(1877..3013)
                     /locus_tag="ACHA6Y_001897"
     CDS             complement(1877..3013)
                     /locus_tag="ACHA6Y_001897"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8987983.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDIVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(3045..4496)
                     /locus_tag="ACHA6Y_001898"
     CDS             complement(3045..4496)
                     /locus_tag="ACHA6Y_001898"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8987984.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(4540..5304)
                     /locus_tag="ACHA6Y_001899"
     CDS             complement(4540..5304)
                     /locus_tag="ACHA6Y_001899"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM8987985.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(5332..6030)
                     /locus_tag="ACHA6Y_001900"
     CDS             complement(5332..6030)
                     /locus_tag="ACHA6Y_001900"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM8987986.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(6042..6821)
                     /locus_tag="ACHA6Y_001901"
     CDS             complement(6042..6821)
                     /locus_tag="ACHA6Y_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM8987987.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(6837..7781)
                     /locus_tag="ACHA6Y_001902"
     CDS             complement(6837..7781)
                     /locus_tag="ACHA6Y_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8987988.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7827..8606)
                     /locus_tag="ACHA6Y_001903"
     CDS             complement(7827..8606)
                     /locus_tag="ACHA6Y_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987989.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2510 a         1838 c         1310 g         3160 t
ORIGIN      
        1 ataatccacc atcatatcat tatttttcca ataatttaaa aaaaagtctc taattatagc
       61 aaacatccat ctagactctt gattacatgc taatgaataa cccgcaaaat atcctgatgc
      121 tacggatgcg tgagcataat caggcctcct aatagaccaa atatcgatat caaaatactc
      181 atctggtatt ggttttgtac aataaaatgt ggcgtctaac cacaaacctc catgttttgc
      241 tagcaaagat aatcttaata aatcagaata atttgttctc gtaatgattc ctttgttttt
      301 cttttcttcc acccattttg gtatatcaac atattgtcta taattatctt ctgttaaaat
      361 tataacagta tgtttccctg catttcgttt tattgatttt acacaagctt ttacaatttc
      421 cggcgattga tccaatccct gccaccaaca aacccaaatg cagttagaat atggtgattt
      481 tgattgatta attatttgat tttgactata atcatatgtt ttcaaaaaat ctgaaaaagt
      541 cttttcataa tacttcagca ttacctcatg tttttttaat aaatgttcct tctgtttttt
      601 actttcatta tatccattac gattcatgac ttgaatatca attttagctt gtaacgtggc
      661 aaatgcttcc ttccaagatg ttatctttgc aattcgtctt gttgcattca attcttcgga
      721 tatgcgatgt ataattcttt ttatacttga atgtttctgt gtcatattct ttaacctctt
      781 tgtattaatt caaataactc ataatatttt gattctaaga ttttgtatgc tgtttgaata
      841 ttataatctg cctttattaa aatcgaacgt tctgaatctc gatgagagat atctacattt
      901 gcaatctctc tcgcccattc ttttgccgat tgatttaaac ttatgaaatt ggtcttatca
      961 ctgaaagcaa cttctgtagg aactttctcc gaaaagaaac acggtaaatc tgcaaattga
     1021 gcttcaatac ccactaacgg aaccccttca aataatgatg gcaacattaa tacgtcaaat
     1081 gcttgataaa atttttctat atcatttcta tttcctaaaa atttaatatc atcatctatg
     1141 ccacttttct ttgcataatt ttttatttct tcttccatat caccagttcc aataattaat
     1201 aatacggaat tattcttaat agcgtgaaat tctttaaaaa tttcaatcaa aaaacgatga
     1261 tttttagaat atgaaattct tccaatatgt cccattacaa acgtctgact tgaaattcct
     1321 aatttcattc gtacatcatt tctaatactt tcatcataat aaaatttttt agcatccact
     1381 gcatttggaa tcaaagtgaa tttatcattt cgaaacaaaa aatcacctgc tacttttcca
     1441 catgaaaatt catgtgtcaa aacagacgtt aacttacatc gataatattg ctttaaaaga
     1501 tatttaaaat ctctatctat cgatgtatta tggctatgtg ctatccgtat cggaatccca
     1561 gctttttttg cagcttttaa aggtaaataa ctcatcgcat caatatgaga atgaacaatc
     1621 tcatattctg gatgatcctt aaaaaattgt tccatccatt taaaatattt aggataattt
     1681 tgtggataaa gtctcggtgc ataatagacc tttcctccaa gacttattat ttcctcatca
     1741 taatcacttt tatatggacg atgtgtcaaa aaatcaaact gaattttagt tcgatctata
     1801 ttcctataat agttcattag catcgtctcc aaccctgcac gatgcatatc attcacacac
     1861 tgtaatattc gaatcattat atatttcccc tatacaaatt taatgtttct tgtacgattt
     1921 tatcccaatt atatttacta caaataaact cagtagcttg attcttcaaa actttaacca
     1981 tttccgattg attgcatgcc tcttctaatc ttatctttaa atcacttaca tccgatttct
     2041 taaaaatcat agccttgtct tctactactt ccgtacactc tgagatattt gaaactatac
     2101 aacagttccc ataactcatt gcttctaaca aacttaatgg cattccttct aaatcacttg
     2161 gaagtgtata gatatacgca ttactatata attcttctaa ttcctgtcct tgtacaaatc
     2221 cagtaaatat aattcgctca tcaccttttg ctaattcctt tagttcattt gcaaattcat
     2281 ctgtatcaga gcttcctccg gctattataa gttttttatc tgtctgaaca tctttaaatg
     2341 cctcaattag ataccgaatt cctttttcgg gaaccaatcg ccccaaaaac aaaatataag
     2401 aatcttttgt taatccatac ttttctgtaa tcaatcctgc tgattttatt tcaatttttt
     2461 taacgccatt tggtataaac cttgtttttc ttccatatat atcttcaaaa taattttgga
     2521 cgctttcact aagtacaatt atttcatctg caaattttac agcacacttt tcccctaaca
     2581 tgatgtatgt actagcaagc ttgttccatt tttctctttg atgatctaga ccatgaattg
     2641 ttgcaacaca tcttttccca aaaagttttg gcaaccacaa catagcacat ggtccttctg
     2701 catgaaaatg aacaatatca tattttccga atgctgctct aattccacca aatactgaag
     2761 aactcattgc tgcaattcct tttatattta atgtaaaaat agtctttaat tttacacctt
     2821 tatattcttt atttttatgc tggtcaaatt cctttccgct aacatgatga ccacttcgat
     2881 tatagcaagt cacttcatgc ccaagtgcaa ccattcgcgt tgccaattct tctaccacta
     2941 tctcaattcc accttctctt gatggaattc ttttatgacc tagcatacag atttttaatt
     3001 tttttcttgc catcacgttt tactccttct atagtatctt tatcttacat ggaaccgttt
     3061 ctatgaagca cagttttgac tgtcctaaac aaaattctaa aatctaatcc catactccaa
     3121 ttcctgatat attctgcatc aagttttaca acctcttcga agtctgtgat atcacttcta
     3181 ccactcacct gccacattcc agtaatccca ggtttcatcg caattctcgc acgatgatga
     3241 aaatcatact tctcatattc tgacttcaag caaggtctaa accccaccag gctcattttt
     3301 cccattagga cattataaaa ttgtggaaac tcatccaatg atgtagatcg aataaattgt
     3361 ccaattcctg tcttctttgt tccatcagga agtattttat tgccaatgat tcttgggtca
     3421 aaatccatct taaacatgag attattactc atcttatttt ctttcatcaa ctctgctttg
     3481 cgttcttctg catccatata catactacgg aacttataca tcttaaatgt cttaccattt
     3541 tgaccaatac gttcttgtgc aaagaaaatc ggccctggtg atttgatgta aataactggt
     3601 gctacgaaga taaaaataac acctgttgca atacatccaa taattccagc aagaatgtca
     3661 atcgttctct ttataaatgc ttgcctcatc gtcataacat taatactaga tgtaataaca
     3721 gtataatcac caactcggcc aataaattgt tttccacgtg tactatcaga cacttttgta
     3781 agattcatat gtacagcaag tcccatttcc gaacatctgg cgattaattc tttgggatat
     3841 ggaacacttt cgtcaacatt gatgaatact tcatctaccc aattctgtaa aagatattcc
     3901 gctgcattat cagcatttgc aactactggg acacctccga tggtagttcc atacatatct
     3961 ctgtctacta caatcacacc actaagttta tacatgccat aattcttctc tttaagatta
     4021 cgaactacat tttttgcaat cttttgagat gttacaataa ctaacgatac atcttcaccg
     4081 tttttcatgc ggtgatgcaa caaccctttc caaagtatac gaattatata tgttaataat
     4141 ccatacacca cccccattat aaataaagaa actctggaat aatcatttcc atcctgcatt
     4201 gtaataaggt ataaactgca cacaagtaac agcataaaag attgctttat agaagctaca
     4261 gcctctttat aatatcctct ttttaaaaca tttttatatg cctcaaataa aaatataatc
     4321 gtaagattct caagttctat aaaaattgcc atgtggcgat aaataggaat cacatacgga
     4381 ttccagtcac cctgtcgcat tacatatgat atataaaaag caagctggac acaaaataag
     4441 tctaataaaa taaaatcaat atgtttagac caaccacttg agttttttct atacatctct
     4501 cactctctcc tcatttcctt tttatctacg aataaagaac taaaataaac caaacttctt
     4561 cttttttacc tcttcataga ttggataatt gatctcatcc ccgttgatca gatcctttgc
     4621 gacttgtttc atctgtacta ctttttcctt accaaactcc ttggcaatct gctcatagca
     4681 ctcttttaag taaaaggtgc gcttagagac cccgtgtgca tcagaagcag ccatctgcac
     4741 gagattatgt ttgaccattg ttttggctgt tttctggata tctttgccaa atacaccaac
     4801 ataacttggc gcagtaagtt gggccaaaca tcccatatct aggaacggaa gcaaatgatt
     4861 tgggtcttct cgaaaatgcg cgttgcgttc tggatggacg atcactggta tctttcctag
     4921 ttctaacaac tcaaaaaaaa gccgttctgc atataacggc acttccagtg tcggaaattc
     4981 gatcagaaga tatgtatcat ctaaatctgt aaataaaatt tcatctcggt ggatgtcttc
     5041 gatgagcgtt ccggtaatac gtacttcttg tccttccaat aacgtcaaag gcaactgtct
     5101 tttctctaat tcggcttgta acgaagcgac tagagaaatg acttgtgatt tttcattgct
     5161 gtattttcca ttattgtggt gtggtgtgca taaaatatgc gtaatgcctt gctgaatcgc
     5221 tttttcagcc attgcaatac ttgcttctaa gttttctgcc ccgtcatcga ttcctggtag
     5281 gatatggcaa tgtagatcga tcatttctct tgtctcctct cttcttattc cttaatttcc
     5341 gtagtaataa ccagcatctt tgctgtgttc tgctccgttg tatacgacac ctaagactcg
     5401 tgcttgtacc atatctaaca gttcttttgc tttagtcaat gattcttttc gtgacgtatt
     5461 ttcccgtacg accaataagg tgccatccgt ttttgaagac atgatctgtg catctgttac
     5521 ggcaacgact ggcggcatgt cgaagatcac tacatcaaat agttgtcttg cttctgctaa
     5581 aatctgatcc attcttggcg aacttagtag ttctgatgga tttggtggtt taggtccact
     5641 tggcagaatc gacaaattgt cgactggtgt tcgttggatc tcatctgcta cacttcctga
     5701 tgaactaaga acagtactta atccgcttgc gttgttcaac ttgaacgttt tatagatgac
     5761 gggcttacgt aagtctgcat cgactagcag cacccgttga ccagatttgg caaaaacaac
     5821 ggcaatgttt gctgcagtgg tcgatttccc ttctccaggt cctgatgaag tcacaacaat
     5881 cgttttgatt tgctgaccag ctgcggaggc aaactggata ttcgtccgaa tcgtacgata
     5941 ttgttcagcg attggtgaag agggattggt gagtgtgacc aaactgactg catgagtttg
     6001 ggctgctttt tgtttctgtg ttcgtgccat tttctttcct cctagactct tgagcggctt
     6061 ctgcgtgatg gcgctgtttt atcgtttgta gcttttggtt gtgtctgagc tggtcctttt
     6121 ttacggatac cagcattcaa ttctttcgct gacatttctg gaactgttcc taagattggg
     6181 aatcctaatg tatctgtcac atatttttca tctttcactg tccgatcgag taattccaat
     6241 aagaatgcca gacctacacc taccatcatt cctaacatta agccagtcat taaattcaat
     6301 ttattatttg gcgaaacagg tgtcgtattg gcaactgcac ctgaaatgat cgagatttta
     6361 tcaacattca tcacatcttt tgcgttctct tggaaaactt gtgaagttgt gttagcgatc
     6421 tgttgagcag ccactgcatt cgtacttatt gcctgaatcg agaacatttg tgaattttct
     6481 gcctgattaa ctgagatggc atctttgatt tgttcagcag tcattttcac attgtcttct
     6541 gtttctaaac gatctttgac ttcattgagc actaaatcac tgacgatcat atctttatac
     6601 gtattgatca tttgcaggtt cgtattgaca tcattgacat ttgcagtgtc tgattgtggc
     6661 agtgtcacga tcaattgtgc ttgtgaactg tattttggtg tcaagatgaa aaatgttacg
     6721 attcctgaga tggccaagcc agcaaacatt gagatgatga ttattaccaa gtgtttgcgt
     6781 aatgtttcaa aaatttcttt caagctgatt gtctcttcca ttttttctcc actttcctat
     6841 ttattctcta gattcatttg atcttttaat tcttttttca cacgatcaag ttcttgatca
     6901 ctgacttttt gataggagat attatcttgc atgaagcctt ctccttgcag ctgatcttgc
     6961 ttaatcgtac caaatgcctc ccgataatct aaagcaattt ttttcatttc atcaaagttc
     7021 atgtctgttt tcatattcga ctctagagca ttcaaaacgg attgatagtt gctgacaccg
     7081 tttagactca atactttttt cgctacgcct tctacgatct ttctttggcg cccttgtcta
     7141 ccgtaatcac catttgggtc ttcataacgc atacgagaat aagataatgc ctgatccccg
     7201 ttcaatgtaa ttttaccgat tggaaagtca tagccatctt gtgagaaagt gaggtcatta
     7261 ttgacttcga taccaccaac tgcatcaacg agttccttta gccctttcat attgatagag
     7321 acatagtgat cgattgggat gtctacatat ttttgtacgg tatccatcgc catggcagct
     7381 ccgccgaatg cgtaagcgtg gttgatcttg tcttttgtgt cacgacctac gatgtcgaca
     7441 tacgtatcac gaggaatgct gacgatcgtt gtctgtttgt cttcaggact gactgtggca
     7501 accatcatcg tatctgatct tccttgatcg acccgaccta aatctccagt atcaatcccc
     7561 atcaatagaa cagagaaaga ttcttgtttg tccagatcaa ctggtttcgt tcgttctcct
     7621 tgggttcgtt ccactgattc gtaagttttc tcaatagaat gtgagagatc cagatataat
     7681 tttgctccta ctcctacaat tgctaatact aatacgataa gaactgctaa aataatcaga
     7741 aatatttttt tccctcttga ttttcttctt cgtcttgcca tagtttcctc cgtttgctaa
     7801 ccttgcttga atcaacgttt taactgtcaa tagcttatat tgctatagcc catttgctca
     7861 gcaacagttt gtgcttttgt tgcgtatgct gcgcttattt tttcattccg gatctgctct
     7921 atttgactga gcaccgtcaa atattgatcg taagtagcgg tggcagtcac ttgaccatcc
     7981 tttaacatcg tatcgaataa ctgatctagt tgcgtgactt gcgcgatctg aatatcagcg
     8041 aatcctagat attgcataac aaccgttttc caaggactat ctttaaaaaa gctcatattt
     8101 tcacgtacac gagcgacatc tgcacttgtc accttttctt tgatgatgac atcgtctaca
     8161 gcttgctgcc agtttgatac atttttagta aataaatcgt tgacagctgt ctggatcttc
     8221 agttttgatt ctgcactatc taactgtgtc acgattgctt tcttctcatc ttggattgca
     8281 acagcttctt taggcaattc tgattctttg atgtcgtaat catctgctga aagtttgaca
     8341 gaggatagct tatttgacaa acttgtgatt gtttcttctg ttatttcagg gtttagaaaa
     8401 attttttctt ttgtactata cagcttgtct acttcttttg ataaagaagt caaggtgtct
     8461 ttttgattgg tgatggcaga ttctgcataa ttgatccgtt gctgatgttg ccgctgatta
     8521 ttgatatggt atccaaacaa acagatcgca atagcacctg cacctaccgc cacagcagtc
     8581 aatgacttga cgaatggctt ttccatagat tcactcctaa gtattgatga tagataaatt
     8641 aagatataat tcacttacat aaaaatgaca gctttttatt taaaaaaaca taattaaact
     8701 aatatattca cagaaatatt tgtaaacaag ttttccctat ataatcatac cacaaactgt
     8761 tttcttgact agggcaattt acgatttaaa agtaaataaa acattaataa gataaaaa
//
LOCUS       DAYYHX010000113         8786 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000113 DAYYHX010000000
VERSION     DAYYHX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.114"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..299
                     /locus_tag="ACHA6Y_001904"
     CDS             <1..299
                     /locus_tag="ACHA6Y_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8987990.1"
                     /translation="DYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPV
                     RTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(396..830)
                     /locus_tag="ACHA6Y_001905"
     CDS             complement(396..830)
                     /locus_tag="ACHA6Y_001905"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8987991.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1062..1772)
                     /locus_tag="ACHA6Y_001906"
     CDS             complement(1062..1772)
                     /locus_tag="ACHA6Y_001906"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8987992.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1762..2622)
                     /locus_tag="ACHA6Y_001907"
     CDS             complement(1762..2622)
                     /locus_tag="ACHA6Y_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM8987993.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2627..3265)
                     /locus_tag="ACHA6Y_001908"
     CDS             complement(2627..3265)
                     /locus_tag="ACHA6Y_001908"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM8987994.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3280..3579)
                     /locus_tag="ACHA6Y_001909"
     CDS             complement(3280..3579)
                     /locus_tag="ACHA6Y_001909"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8987995.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3622..5106)
                     /locus_tag="ACHA6Y_001910"
     CDS             complement(3622..5106)
                     /locus_tag="ACHA6Y_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8987996.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5126..5587)
                     /locus_tag="ACHA6Y_001911"
     CDS             complement(5126..5587)
                     /locus_tag="ACHA6Y_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8987997.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5605..6672)
                     /gene="ulaG"
                     /locus_tag="ACHA6Y_001912"
     CDS             complement(5605..6672)
                     /gene="ulaG"
                     /locus_tag="ACHA6Y_001912"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM8987998.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6890..7660
                     /locus_tag="ACHA6Y_001913"
     CDS             6890..7660
                     /locus_tag="ACHA6Y_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8987999.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7933..8760)
                     /locus_tag="ACHA6Y_001914"
     CDS             complement(7933..8760)
                     /locus_tag="ACHA6Y_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8988000.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2656 a         1852 c         1383 g         2895 t
ORIGIN      
        1 tggattatac tgttgaattt gacagtgaac tacttaagca atttccacaa gaagaacatt
       61 tcatgtataa agaagcaatt tcccacaaag tgacagagta tttggagcat aatttttttc
      121 gtataaagcc agtgcgtact cgttcggcaa aaggaatata cgagatgaaa gtcaaaatta
      181 ataaaaaaaa ttatcgcata gcatttgctt taaaaaaaaa tcatgtgttc attttttata
      241 ttagttcgaa tttgcaaaaa attgtatttg ataaagaggt gagtaaaaaa ataaaatagc
      301 tgagcattgg aggatagatt gagtgatact aagtctatcc tcttgttttt ctaaagaact
      361 aagctagaat tttttctttt gtacgttatt atatgttatt tgtcattacc aaaaagcacc
      421 ttttttcttc tagtatctat gacataagaa ataccaccca tttcctttac ttgaacaaat
      481 cctactgatt cataaaattt ttttgttttc tcagtatcat ctgtcaataa ttcgatttgg
      541 actggtccat aactagtgat cttctccaaa gcttcattca aaagcacttt gcctattcct
      601 tggcgctgat acctcggatg aatgaggata tcttgaataa atgcgatcgt cgcaaaatca
      661 gtgatccaac gaacgattcc gataatcttc ccttccccat tacgctttat cagagattca
      721 gattgttcaa atgctctagc taaccttgca gtgtcttttg tgtaattcgt ccaaccaacg
      781 ctcttatata attcagaaat ctcatcgatc ataagggaat caatattcat ttttttcttc
      841 ctttcttata caattctccc tctaataata cagttctttg actatttaat ctatcacata
      901 taactctcct gtaaagattc tctcgattcc ttaaaattcc tctttccttt tagaatgcat
      961 actttttgat aaataaaaag gttgtgataa attttcttca caacctctgt ttctctctat
     1021 caagtgttat cttttaattt tctaccctta ccctagcatc tctattgccc gtagtatgca
     1081 tactttccat gtttgcgaaa ataatgttta tctagtaaat attgcgggat ttcacatata
     1141 ttggggttga tcagctcgtt ttctttggcc atcagacaaa tttcatctaa gatcaaactg
     1201 ttttctactg ctttcatagg tgaatccccc catgtgaacg gtccatgtcc atagacaagt
     1261 actccaggga cttcgtttgg atctaatttt ctttccttaa acgtctcgac aatcacattc
     1321 cctgtatgaa cttcgtaagc ttctcttact tcttctttcg ttaattggcg tgtacaagga
     1381 accttaccat aaaaagcatc agcatgagtc gtgccataag ctggcaaatc gcgtcctgct
     1441 tgcgcccaca tgacggagtg cgtggaatga gtatgagtaa tagcagttat atcttcaaaa
     1501 gtttgataca aaacaacatg tgtggggaga tctgaagatg gttttaagct atcttcttct
     1561 attatatttc ccgacaaatc tgttaccacc atttgatctg cttgcatgca ttcatatctg
     1621 acaccgcttg gcttaatgac gatgactcct agctcacggt ccacttcact cacattgccc
     1681 caagtcaatt tgactaatcc gtatcgcggc aatgccagat ttgcttcaag gacacgttgc
     1741 ttcattgctg caattttttc ttcacaagac atcatagcca gcctccttca atttagaact
     1801 taagtagtct tttgcctgct ggatttctct tcgaaagtct tggctttttt cactccacat
     1861 ttcaatcaga aatgttccat cataatctag acgtttcaaa tgtcttaata atccaagaaa
     1921 atcaacacaa ccgctaccaa aaggaacatc acgaaactgg cctgaatatt cttttgttac
     1981 agggtaagta tcttttaagt ggatagcagt gatataatcg atccctttct caagttctcg
     2041 agccggatta ttttccggcc atgcggacaa attgcctagg tctggatata cttgtagaaa
     2101 aggtgaatga atctgttctt tgatttctaa aaattttgaa atggagttca taaatggatc
     2161 atccatgatt tccacggaaa gaatgatgcc atatttagaa gcttccttta cgctttcttt
     2221 tattccttcg ataaagtctt ctctggaatt cattgatttc tcttcataat aaacatcgta
     2281 tccagctatt tggattactt tgacgcttag attatgcgcc aaatggattg cttttttcat
     2341 tagttgtttc gctgcttttt tcttttctgg atcagcagaa ccaaacggaa atcgccgatg
     2401 cgcacttaaa caaatcgaat ggatacgtac accggagctg aacatctctt ctctcagctc
     2461 agctatcttt tcttcagacc aatctaatcg tgctaaccgc tcatccgtct catcaatcga
     2521 catctcgata aaatcgaagt ccatttcttt tacaagggaa aaacgttctt tccatgaaat
     2581 atctttgggt aacgcttttt cataaatccc aatttgagcc atttccttac ccccagatac
     2641 gaatgatctc atctttgaag ttttgtgcgg ccttcatcgg atcatttgct gctgtaatac
     2701 cacgaccagt gataaaggta tacacatcga tcccttcaaa taagtgtaat gtgtgtggat
     2761 ttaatccacc agttacagaa acattaaagc ctagttcaat cagttttttg atcttcgata
     2821 aatctttttc accccaagtt tcaccagata acagcgcatc acggctttga tggtagatta
     2881 cctgccggat accgatgtta tgccaatcca tggcttgttc aaaagtccaa ttgccgtaga
     2941 gttctacttg caattcacct atttcttttt gtgctgcctc catagttgga atcgttgcac
     3001 aacaaatgac tgtcatccag tctgctcctg cttttgcaac attactcgcc actgttcctc
     3061 cagcgtctgc acatttcgta tcagcgacta gttttttatc tgggaacata ctgcgaatac
     3121 agcgaatcgc tttttgtcct tcctgtaagc aaagaattgt gcccacttca ataatatcta
     3181 ctacatcacc aactttcata acatctccta atgcatgttc taagtcattg tgatctaacg
     3241 caatttgcaa attcggcctg ctcatcttag ttccttcctt tacttgtttt tttggtacca
     3301 ctttgtttca gcaaattttt cactgatttc tttgactgac atcacatttt tgataccgat
     3361 aactgtaact ccttttttct ctgcctgagt aaacatattc aaaaaattga ctggcgtaaa
     3421 aactacatcg tattggccag ctgaactttt tccctctgaa atggcacaat gatggatttt
     3481 ggtaattggt acgtttagtt ctttcattgc tttttccaca cttctcatca tcatcaagct
     3541 tgttcctgat ccatttgcac acgatactaa aattctcatc ttaatccctc cattatgagt
     3601 gttgtctaat cagattttct tttatactct ctgcttttct gcatatgtgt catagtcttc
     3661 tacaatcaaa aaataaccat caggattttt ccgatattgt aactgcggaa tagccaataa
     3721 accaatgaca actacggcaa ttccagcata acctaggact ttcatgatta ctgtaaacaa
     3781 tggccaagct gttgcccagt cgaacatccc gatataacca ccgtatgttg acatcccaat
     3841 ccatgtagcg aataatgctg aaccgcctac ttgtatgatt cctgaaagaa acgggaacag
     3901 gcaagcggct ttgaatccac ctcggttatt ggcataaact gctataacgg cattatcaaa
     3961 gaacaacgga atgaaaccgg ctatgacgat cgttggtgat ttcaataaaa tcagtaaacc
     4021 aatcatcaaa aactgtccca aagcaccaaa caagaaccca atcgttaccg catttggcga
     4081 accaaagcca aaagtagcag ctacgtcaat tcctggaaca gctcctggta aaatcgtgtt
     4141 tgagattcct tggaaagatt gagttagttc atcgacgaat gtccgaacac ctaattgcaa
     4201 gatcgccaga tataccgcaa aatttaaaga agtcgtcata atgtagaata agaaactctg
     4261 cccttcttgc atgaattgcg cttgaatcaa ataatctttg cctagtacaa tcagaatgat
     4321 cccaaagaaa aatagcatga gtaacgaagt agcaaccata ttctcattga aaatcgacaa
     4381 gaatcctggt aatttgatat cttcaatttt tttgtcttgc ttgattgatc tatttttctt
     4441 ttcatcccgc tttttcatcc actcagctaa ttttgcaaat ataaagattc cgaacatttg
     4501 ctgatgtgcc actgcaaatc cggcaccttc tgtcaattcc tgcgtgattc ctactgttaa
     4561 gttagaagct actgtccaat aacaacctaa aatcagtccc ataaaaatca atgcttgtat
     4621 cctacctaga tcaggaaagc aaaacagtaa aatccaaaaa gcggtagctg cttgttgaac
     4681 ctgaacgttt ccggtagtga aaacggaacg cagtttcgta tatttctgaa aacggactaa
     4741 taagatattt aaaacaaatg caatcagtaa taaaatcatc acatctccaa aggtacggcc
     4801 aaatgtctcc attaatccag catccacagc attttgtcca aaataaggat ctgtaaccat
     4861 ggcatccaaa ttgaaccgtt cctttagtcc aaccaagatt ggccgaaaat tattgaccaa
     4921 accaccagag ccaacagtca aaataaagta accgaccgtt gcctttaaga atccagcgat
     4981 gcattcatga agcgggcgtt tcagcaaaat atagcctaat aacacgataa aaccaatcag
     5041 gtatgctggc tgcgtcaaga tatttgttgc aaaatacgtc caaatgctta acaaaaattc
     5101 ccccatcttt tttctcctct atcaattaat tatattctgc catcacaact tggtaatcat
     5161 cgatggagtt cgtagagatg agacgctcaa ccaatccatc tgtcattaat aattcggata
     5221 atttttggat attttctaga tgttcttctg gatcttttgc tgcgagtgta aaaaacaaac
     5281 tagccgtttt ctcctcattc tccccttcac tttcaaaacg gattggctgt ttcaacttgg
     5341 taaatgaaat agctgttcca aaaacaccat cacttttttc tgaagaatgc ggcattgcta
     5401 cttgaggtgc gatcacaata taaggaccgt atttttctac acaatggatg atctcctcca
     5461 catacgtttc atcaataatt tccttatcga tcaatacaag acagctttcg gcgatagctt
     5521 cacgccaatc cgtgatttct ttatctgggt aatgaatcaa ttgatggtca aaaaagtatt
     5581 tcagcaagaa actccccttc tttcttataa aaacgacgga aacggtgtat cattttcaat
     5641 cgagaagaca tcttcaaatc ctcgataaaa attaaactca agatcatctt tgtttgtcgg
     5701 atagatgaat ttcccgccta cttgccagat ataaggttta aatccatact tcaggcgatc
     5761 ctttttgaat ttccaaattt cagtgatctc ttttggatca gccataaaat tagaccaaat
     5821 atcgtagtgg acaggaatca ctacttttgc attcaatgac tccgccatgc gcagcatatc
     5881 aacagaagta actttgtctg ttatccctcg tgggttttca ccataggccc ctaaacagac
     5941 atcgattttg tgttcattac catgtttggc aaataaattg gagtaatgtg aatctcctgc
     6001 atgataaaga ttccctcctg atgtttcgaa taaatagttt actgcaattt gatccatctc
     6061 ctgaggcatt tttcctttta atacctcttc tggatcttca caagtgacca acgctgtccg
     6121 atcaaacgct tctaaagcta ctacattgat gtctttgatt ttgactgcat cacctggctt
     6181 tactgtaatc gttttttctt caggaattcc ccatttttgc cacgtgcgaa ccacttcttt
     6241 tggcccaatg aatgttgctt caggacaatt ttgatgaact gctgcggctg tatggatatc
     6301 tagatgatcg gaatggatat gtgtaacgat taaagcgtct acatttttta tcgcaaaagg
     6361 atcgattacg aaaggctgag tacgtaaatt aggctgcata ttttgaacgc cactcatacg
     6421 catcatctga tggccttttt tcatcatgcc attcccatga ctacgttttc ccgtcccaca
     6481 ccatagatca cataaaatat tcgttccttc atgacttttc agccaaattc ccgtacagcc
     6541 cagccaccac atagcaaaag ttccaggatc cactacttct gtttcgattt cttcattcaa
     6601 gtatgtgccc cactcaggaa atgtttctac taaccacttt tcttttgtta cttcattgac
     6661 agttttactc atttgcttgc tccccccata tcgttgttcc aacttacaag taaatattaa
     6721 tgtgtatcag catccatgaa aaccctttta tatgaccgtt tgttttttat atgattttct
     6781 ttcagtaatc aatgattgtt tcagtataat aaagacagat atgaatgttt aaatgaacaa
     6841 ctagtcaaat aaatccatgg atcaagatgt ccctcaggag gcaaaagtca tgaaaaactc
     6901 acttgtcaat attgaaaaac gtcaaaaaga aattttggaa ctgcttcagc agactgaaca
     6961 actatcaact ttcgaattag ctgaatccct tgatgtatct attagtacga tacgaagaga
     7021 tttaaattta ttagaagaaa aaaacgagat catacgcaat tacggttatt gttcttataa
     7081 ttacaaaaac cagactgatt ttgatcaatc tggtcctgaa aggattaaac aagcgattgc
     7141 cagagaagct agtaaataca ttaacgatta tgatacgctg tttatcaatt ctagctcgac
     7201 tgcgttgaaa acactggatt ttcttcaatc taaccatttg acagtcatta caaacaacgt
     7261 gaaaatcaat tactctccgc acaaagataa ttgtagttat attttaactg gtggagagat
     7321 gcgctttcca aaagaagcac tagtgggcga catcgcatta aatacgattt cttcagtcaa
     7381 tgcagacatt tgtatcattg gctgtagcgg agtcgatgta gtacatgggg tcacaacaaa
     7441 gatattaaat gaatcaacga tcaacgaaat gatggtcaac aagacaatca aatgtaagat
     7501 tttagtcgct gatcatcgaa aaatcggact gacatcaaag ttcaagattg ctgatatttc
     7561 agtctttgat tatttgatca ctgataaatt ttgttctgaa aaagtattag aggagatcag
     7621 aaatacgggg ataaaaacca ttcaagtaga ttcattttaa gaaccaggac gtaaatatgt
     7681 aaataggatt atcaaaaaaa tgaataacaa atttatcgat aaatgaaaaa cacgctacgc
     7741 tcaaattgat aaaggaatcc caagatgtat ccatccaatc gataccatca taatctattg
     7801 cttcttttat aaccgttata tgttgcctaa ataggaatca aacaatatcg aaaaaaggag
     7861 gtatgacaaa agtcacacct ccttttttcg aataaacggt attcaaatca gaacactgat
     7921 ttcacaacca ctttatttca ggtcgttttc aatcaccttt accgcgtaat catttccgtt
     7981 gaaacgcgct attttttttg cgtccatata tctttctaaa taatcatctt ctcctaattg
     8041 cttatggcaa gaagcaattt cataaagcaa gtattccaat gcataagtcg atttatgttt
     8101 cttattatac tcaataccct tctcacataa ttggattgct ttatcataat cctcgatttt
     8161 acggtaatag gtggcgatat cataatatac aatgcagacc atcagattat caaaatcagt
     8221 ctcactcaat attttaagtg tacgctccac ataaactttc gcaaaatcta attctccttt
     8281 tgacaagtag atcattgcca cgtttgcagc tgcttgtaag tgatggatat ctgtttcggt
     8341 cgtttgttcc aacacttgat taaaataaaa taatgctgca tctggttttt tcgcataaag
     8401 atgatcaagc agtccttgat aaaataaaat ttgatttaaa agatcagaat tacttacttt
     8461 ttcctgatcg atcgtttgta aaattttata agattcttgg tattttgata cagaattcaa
     8521 tgattgaatc tcatttattt ttttcctgat tttgtcttct tctgtttcta ctaatatatc
     8581 ggagatctcg acattcagct ttttgcaaag cgcacttaag atattgatgc ctggcgacat
     8641 attcttgttt tcgatattgc tgacagttgc ttgttcacaa atgccttctg ctaattgtac
     8701 ttgtgttaac ccctttttct ttcggtattc ttttagtaat gctccatcta ctttaatcaa
     8761 aacaatttcc ttccctttaa aaaatt
//
LOCUS       DAYYHX010000114         8642 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000114 DAYYHX010000000
VERSION     DAYYHX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.115"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(240..2621)
                     /locus_tag="ACHA6Y_001915"
     CDS             complement(240..2621)
                     /locus_tag="ACHA6Y_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8988001.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2611..3258)
                     /gene="recU"
                     /locus_tag="ACHA6Y_001916"
     CDS             complement(2611..3258)
                     /gene="recU"
                     /locus_tag="ACHA6Y_001916"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM8988002.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3322..3861
                     /locus_tag="ACHA6Y_001917"
     CDS             3322..3861
                     /locus_tag="ACHA6Y_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM8988003.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3951..4370
                     /gene="gpsB"
                     /locus_tag="ACHA6Y_001918"
     CDS             3951..4370
                     /gene="gpsB"
                     /locus_tag="ACHA6Y_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM8988004.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4392..4759
                     /gene="rnpB"
                     /locus_tag="ACHA6Y_001919"
     ncRNA           4392..4759
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHA6Y_001919"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4968..6134
                     /locus_tag="ACHA6Y_001920"
     CDS             4968..6134
                     /locus_tag="ACHA6Y_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM8988005.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6180..7676
                     /locus_tag="ACHA6Y_001921"
     CDS             6180..7676
                     /locus_tag="ACHA6Y_001921"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM8988006.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7757..8440
                     /locus_tag="ACHA6Y_001922"
     CDS             7757..8440
                     /locus_tag="ACHA6Y_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8988007.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8449..>8642
                     /locus_tag="ACHA6Y_001923"
     CDS             8449..>8642
                     /locus_tag="ACHA6Y_001923"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988008.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2715 a         1569 c         1836 g         2522 t
ORIGIN      
        1 aaagactagt gtgtatttag ccgatttaat tataaacaag taaatggata aagtatatgt
       61 aatgataaaa gaaaaatgga ttttctaatt tttaagctaa atgttagatc acacatgtat
      121 atgctggata gtcttataaa aaatcatgta atacagcttg gtacatgatt tttatttgta
      181 gctgtttttt acatagacac aaaaaagaaa ccacgcttgt agacgtggtt tcttttttac
      241 tatctgtttc cagaagctga ggtgctgctg gatggtgtat ggtcatttgc agcatccgct
      301 ccgctagttg ctggctcact ggaagaagca ggaggagcag atgaagacgg tgtcgagctt
      361 tcttcggatg aagaagaagg aaccgttgtt gaagacgctg gctcgcttgt ttctgctgtc
      421 gtgctttctt ttgaggactc agcagttgat tcgctttgac tagtagagct agagctttga
      481 gtcgttgtct ctgttgttga gcttccagga gtctggacat agctggaaga tggaatagtt
      541 gttgatggtg taattgcatt cgatctagct gtatattgat ctttgtagta cagttcaccg
      601 cccacacgaa ttaatccgct tggcatctcc cagtctgtat ttgtcacgtt agctgaaaca
      661 tactgcataa gctcgcgata aacatcagag gctacgtgtg acgattctga agtgacaggc
      721 gtcatttttt tattgtaacc tgtccacacg gaaatcgaat aatttggcgt atagcccgca
      781 aataggatat ctggatacac cccagaagaa ataccaagtt ttgcatactc gtcatctgtg
      841 taattggatg ttccggtctt accggcttga tataacccag cgatttgcgc attcgtacca
      901 gttccttcag tgatcgtatc ttttaaaatg tctgtgatca tataagctgt ttctggagac
      961 atggctgttt ttccatctgg ctcataagtt tcttctgttc cgtcttggaa gacgatttta
     1021 ttaacatatt gaggtttgta ataagtccct ccattggcga aagccgcata ggctgctgcc
     1081 atttttaagg aagaagctcc gtattttgtc ccatcttgtt cttccgtatt actagagatc
     1141 gcattcgatt gatggatcgt actgtattcg atcccaagat tttttaagaa agaagccact
     1201 ttatctgaac cgacttcatt gaataatttg actgctggaa cattacggga taagtaaagt
     1261 gcttggcgta aagtaatggt gcccatatat tgattatccc agtttcctac aggagtcgaa
     1321 gtcccttcat agttatacgg tgcgtcagta atagtttttc cagtagaata tttcaaatat
     1381 tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag aaccaaaatc acgggaagta
     1441 ttcactgcta agttgttgcc taaagttacg tcttctgcga tgtgacgtcc gccaatctgg
     1501 gcttttactt tcccggtatt ggtgtcgata agggtggagg caacttgcat ctcgtcgtct
     1561 gggtaagaga catactgatc cgtgttgacg atatcatata gtttcttttg ggcgtccaga
     1621 tccaaattcg tataaatctc taaaccgtct gtgtagacat tcttatctgt tttttcttgt
     1681 acttcattga tgacttcttt gacataatta tcgacgatct tagtattgtc atcggattga
     1741 gttagttctt gcagaccatc cgttaccggt acattaacag cttgatcata ttctgtttga
     1801 gagatttttt cgttttgaag catcgtatac aaaacagtat ctcgtctttt cttcgcctga
     1861 tctggataaa cataaggatc ataggcggaa ggggcttgag gcatcccagc aagcaatgct
     1921 gtttgcggta aagaaagttc tgataatttc ttaccaaagt acatctcaga agctgtctcc
     1981 atcccgtaca agccgtttga catatacact ttatttacgt agtaggtcaa gatctcttgt
     2041 ttcgattttt tttgttccaa tcttacagcc atccacgctt cttgggcctt gcgtttcaga
     2101 gtctgatcct cggcactggt ggagaagaag gaaagtttga tcaattgctg tgtcaaagta
     2161 cttccgcctt gcagtccgcc agaggtaaag ttagagaggg cagaaccgat gatccgaata
     2221 gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta ccgacacgat cgcatcttct
     2281 aatgttttcg gtaattcatt cgcagaaatt ttttcacgct tttctgcgcc taaatcttcg
     2341 aatagttcgc catcttgtgt atatagtttg gaagatacgg tagcgtctaa ttttttatcc
     2401 gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc ctacacctgc taaaaagagg
     2461 atacagaaaa atgagagtat tcctaaaagg atcttgataa ataatccttt tttatgtgtt
     2521 ccagaggact ttccagaacc tttgccagaa ttcttcttca cactcttttt aggagtaggc
     2581 tgtttctgtc gtctagagct tctagtttgt tcatttgcca ttcttgcttt ctccttcaat
     2641 agataatgtt gataaataga agtcaaccgc gtccaaataa ggaatccgcg gagaaatacc
     2701 agttttgatc tctattccta gttcttcgat caaagataaa ggtagtgatt tgcgtccttt
     2761 ttccttttgg gcgtcccagt aagcaatcaa atgaacacta ttcaaataaa aacagcgctt
     2821 caaagacgaa aaccataaca agacaaagca gaccccattt tgttcgaggc atgctttcat
     2881 atgttcgatt tgatgggcat ggaagttttt aaagggaaaa gatgttttgt tcttggtttc
     2941 ttttgcttca aagtccaagt aaaagcctcg atagacgcca ttatagtctg tagttgaggc
     3001 ttgggtaaag taggcttcct tgatcacagc agcacttctc ttaggatagt ctactttcac
     3061 gatttggata ggggtaggct ttttatggat aaccgccaac ccgcgggaaa gataatattt
     3121 attactttca ttgattgctt cttcaaagcg catcccccga ttgccgaatt cagtgatctg
     3181 atttcttttt tcttgaattt tttcgacggg agaggcattc ttataataag gttggccatt
     3241 tggatagcgc aataccatct ttaatcacac tcctaggcca cattatagca aaaaagcgga
     3301 tagaaagaaa aggaatctga gatggataag tcaaaagtaa tctatgtgtc tgggtatcga
     3361 agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa tccaagttgt taaaaatgtt
     3421 ttaaaaaaag aactccatca gttagcagaa gaagggctgg aatgggtact tgtcagtggg
     3481 aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg aattgaaaga agaatatccg
     3541 gaactatctc ttggtatttt gtatcctttt tctgattttg gtaataactg gaatgaaggg
     3601 aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt atgtcgaagc tgttagccat
     3661 caaccctacc aatcacctag tcagctaaag aatcacactc gttttatact tgagcataca
     3721 gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa aaacgaagta tttttttgaa
     3781 gatgcacaga attaccaagc agatcatccc tacgaaattc ggctgatcag tatggacgat
     3841 cttcaaaatt ttagtgaata atcgatattg tgtttgattt atcaactttt ttcctataca
     3901 atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag aggtgtaaat atggcgaatt
     3961 tggtttatag tcctaaagac attttacaac aagaattcaa aacaaaaatg cgcggctatg
     4021 atcccgttga agttgatgaa ttcttagaca atatcatcaa agattatgaa acttacagca
     4081 aagaactcct tgctttacaa gaagaaaacg atcgattgag tgcaaaagta gctcaattat
     4141 ctaaaactca aggagcagct caaacacgcg tgcaacaaac agaggcacca aagagcgcag
     4201 cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct tgaacgtgaa gtatttggta
     4261 aaaagcttga tcaacaagca agtgcagtca aaccagcaca acctaatcca aataactaca
     4321 caaatgcaga cacaagctta gatgataatg aaaaaacacg tcaattttaa tttttgatta
     4381 atgaatttgc ggttttcggg taatcgcggt ctgacttgat caggctgagg aaagtccatg
     4441 ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg aaaccataag ctaaggtact
     4501 ctttggagta acggcaggaa aacaagctaa ggcttcggct atgcttaagt atccttgaaa
     4561 gtgccacagt gacgaagcgg ctggggaaac ccatccggtg gaacgcggta aacccctcga
     4621 gcgagcaacc caaacaatgg taggggcgct ttttgtctgg aagaagaaca ggacaaaaag
     4681 gcagtataac tgcagataga tggttaccgc tttgtttttt ctccctggaa aagcaaagta
     4741 cagaacatgg cttatagaaa accgcaaaca tcagggaatt aggtcgtgac aaaagtaatg
     4801 cgttataaga aacaagaaaa acatttgaaa gctatttttc aatacttcag atgaatcgtt
     4861 cttattacaa aacaaacttt tgtatcaccg gttattcgga atttgagtaa aagtgtgggg
     4921 taggactgat gtcccacctc ctttttttat aaataaggag attgtgtatg gaaaaaaaca
     4981 ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga agcactggtt ggtaaagaat
     5041 taagagatct tggaatcgaa tgccaagttg agaacggacg tgcacgtttt gaaggaacaa
     5101 tggaaacgat cgctacagct aatttatggt tacgtacagc agatcgagta aaaatcgtgg
     5161 ttggggaatt cgatgcgtat acgtttgatg aattgtttga aaacgtaaaa gcattgcctt
     5221 gggaagattt tcttccacta gatgcagctt ttcctgtagc tggaaaatcg atcaagtcga
     5281 aactatatag cacacctgat tgccaagcga tcacaaaaaa agcaatcgtt gaacgtctcc
     5341 gtacctatta tcaccgtcca gcttctgtac cactgacgga aactggcgca cattttcagt
     5401 tggaagtagc tttgctgaaa gaccatgtga tggtaactct ggatacgaca ggaccaagtt
     5461 tattcaaacg tggttatcgc ttagaaaaag gtggagcacc attgaaagaa aacatggcag
     5521 cagcacttat tatgttgaca aattggcgaa aagaccgtcc attttatgat cctgtatgtg
     5581 gttcaggtac gctttgtatt gaagcagcct taataggcca taatatcgca ccaggattca
     5641 atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca agaaatcatg gaaaatgttc
     5701 gatcagcagc cgaagaaaaa gcagattatg atattgaatt agatattact ggttcagata
     5761 tcaatggacg gatgattgaa attgccaaag ccaatgcaga agaaatcgga ttaggctctt
     5821 cgattacctt taagcaacaa gcagtcaaag acttcaaaac ggataaagaa tacggggtca
     5881 tcgtggctaa tccgccttac ggagaacgtc taggagaaga agaaactgtt cggaaattat
     5941 ataaagaaat gggcgatgta ttccgtccat taaaaacatg gagtaaatat atcttgacta
     6001 gcgacttagc gtttgaggaa ttttacggac aaaaagcaac caaaaaaaga aaattgtaca
     6061 atggtgcatt gcgtacggat ctattccagt attggggaac tcgtccgcct agaaaaaaag
     6121 agaaagacga ataatcttac taattgattc attcaattgg atgtttggag gaatagggaa
     6181 tgaaagaaaa agagttttta caagaagtaa aagaaatcaa tcttctacaa caagctttag
     6241 gaatcctcga ttgggataca caaacaggta tgcctgaaaa agctagtgca taccgaagtg
     6301 aagtagacag ctatctttac agcatttatt tttcaaaaaa aatcggtcca aaaatcaaag
     6361 aagccattcg ctatttttct gaacatccgg aagaattgtc ggaagttgga actgccgttt
     6421 ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt accggaagat cttatgagaa
     6481 aatatacagc tgccgtttct tcagcacacg gacaatggca aaaagctaga gaaacacaac
     6541 aattttctga ttttcaacac gcattgaatc aaaatattga attaacaaaa cagttgatac
     6601 cttactggag aaaagatgaa aagacgaatt atgatgtttt gctgaatcaa tacgaacccg
     6661 ggatgactgt cgaaatcctt gataaggtat ttgatcagct taaagaagga attctttcga
     6721 tccgccgtac gctccaagaa aaagggactg aacctgaaac agacttttta aatcggagaa
     6781 tgacgaaaaa acaacagaag agatttgtag tgaaagtaat cgaacaactt ggttatgatt
     6841 ttagtcgtgg tcgtttagat gacactgtcc acccatttat gactggaatt aatccgaatg
     6901 atgcacgaat cacgacacga tggaacgaaa cagatttcaa aatggctgtt tttggagtga
     6961 tccacgaggc tggtcatggt atgtatgaac aagacatcga tgataaatat gcgtatacac
     7021 cggtttatga aggtacatca atgggtatcc atgaatcaca atcgttgttt aacgagatca
     7081 ttattggaag tagtcgcagc ttctggaaaa agcaatatcc tttctttcaa gaatgtgcag
     7141 aaggaacatt cgatgatatt ccttttgaca aattttatcg ttcattgaac catacaaaat
     7201 caagtttggt tcgtatcgag gcagacagtt tgacttaccc tctccatatc atcattcgtt
     7261 atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt tgctgaacta cctgaaatat
     7321 ggaatcaaaa atatgaagag tatctgggaa tacgaccaac aaatgatgtg gaaggcatcc
     7381 tacaagacgt tcattggtca ggtgcaagct ttggttattt tccctcttat gcattagggt
     7441 acatgtatgc ggcacaatta cttcattctt tgcagcaaga aatagatgtg gaaaatgttc
     7501 tagcgtcaga cgattattct ccaatcaaaa actggatgtc agagcatatc catcaatatg
     7561 gtgcatctag aaaaccaaat caattaatca tggatgcaac acatgaaccg cttaatccgc
     7621 aatacctgat cgactatatg aaagacctgt attttaatgt atatcaggta aaataaaata
     7681 caatttacaa agatagctga aattcatcta atctatagat gttttcagct atttacttaa
     7741 gaaagaagtg gaacgaatgg ataaattaca agtgatcgct gattatagta tggaccaatt
     7801 agcgcatgat caaacaggac atgggagcga tcataccaaa agagttgtca agttagctga
     7861 aaaaattttg gagacggaac cgcaggcaga ccgctttgtg acgcttgcag cagcttacct
     7921 acatgatacg attgatgata aagtcgttaa aaatgagaac gaggcaaagc aacagcttcg
     7981 tgtattcctt cgctctttgc caatagtaga ggaacagatc tcgatgattt tttccattat
     8041 cgaaaatatg tccttttcta aaaatctctc tgaagcggcc gaactatctc tggaaggtaa
     8101 gatcgttcaa gacgctgatc gtatcgaagc attaggagca atcggcattt tgcgtactgc
     8161 ttatttcggc ggtggacatg ggcacccgat ttttgatcct gaactgtatc cgcaaacatt
     8221 caaggacaaa gaaaattacc gaaaaggcac gacagtgatc aatcattttt atgaaaaact
     8281 gttcttgctg tctgataaaa tgaatactga ttacggatat gaagaagcca aacgaagaga
     8341 aaccttcatg cgtgaattct tagaagaatt ttttctggaa tggtctattg acgatgaaaa
     8401 ttttactcca gacaattgga tcaaaaataa caaaaaatga tggaggttgt gacagaagtg
     8461 gtcagctcca agatatttcg gatcattttc cgtaaaagtt acttctggaa cactgtttac
     8521 caagaggtcg tgagcctgcc gttcagctcc gagtcacaag gaacaatttc aaaaaatagc
     8581 ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat tggcaggaga acaccgttta
     8641 tt
//
LOCUS       DAYYHX010000115         8639 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000115 DAYYHX010000000
VERSION     DAYYHX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.116"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..130
                     /locus_tag="ACHA6Y_001924"
     CDS             <1..130
                     /locus_tag="ACHA6Y_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8988009.1"
                     /translation="QVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            complement(83..295)
                     /locus_tag="ACHA6Y_001925"
     CDS             complement(83..295)
                     /locus_tag="ACHA6Y_001925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8988010.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFLMILFVKLSETKLSIYVTQKNF"
     gene            complement(739..1860)
                     /locus_tag="ACHA6Y_001926"
     CDS             complement(739..1860)
                     /locus_tag="ACHA6Y_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFM8988011.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2188..3597
                     /gene="istA"
                     /locus_tag="ACHA6Y_001927"
     CDS             2188..3597
                     /gene="istA"
                     /locus_tag="ACHA6Y_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFM8988012.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3594..4322
                     /gene="istB"
                     /locus_tag="ACHA6Y_001928"
     CDS             3594..4322
                     /gene="istB"
                     /locus_tag="ACHA6Y_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFM8988013.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4450..5361)
                     /locus_tag="ACHA6Y_001929"
     CDS             complement(4450..5361)
                     /locus_tag="ACHA6Y_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8988014.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5378..6385)
                     /locus_tag="ACHA6Y_001930"
     CDS             complement(5378..6385)
                     /locus_tag="ACHA6Y_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8988015.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6382..8511)
                     /locus_tag="ACHA6Y_001931"
     CDS             complement(6382..8511)
                     /locus_tag="ACHA6Y_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8988016.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8516..8626)
                     /locus_tag="ACHA6Y_001932"
     CDS             complement(8516..8626)
                     /locus_tag="ACHA6Y_001932"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988017.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2652 a         1758 c         1505 g         2724 t
ORIGIN      
        1 ccaagtcaat caaggattca tctcatccgt tgcgtccaaa agaaaccata tccctagaaa
       61 atcactaaat taccaaacac cattagaagt ttttttgagt tacgtaaatg gaaagttttg
      121 tctcgcttaa tttgacaaat aatatcatta aaaagttagt aaataattgc cctctaagtt
      181 ccacggtctg ttcattaata actttatatc ctcgtttttc aaaaaatggc tttgcggtaa
      241 tggaagcgtg ggtagtgatt ttttgcatac cagtttctaa aacatggttt tccaattcat
      301 ctgcaatttt agtcgcaata ccttttcctt gatggctatg tttttgtcac aacttcagta
      361 ttcctgtaac aatacggatt tcagtataca acaacagctt ttgaaaagat acttgcaaaa
      421 ggattaagat atctatgacc tgctatgctc actgatgttg aaaagaatgt tttattagga
      481 ttgaggtaag caagatagtg aatatgtccg gaaagcctgt aaaataaggc ttttcggaca
      541 tatgtgaagt tataaaaaga tagcgaaaaa tctatttagt atcactaatg ttgcgaacat
      601 atcgtcagaa tagtctgaca tttatttctt gctaaaaatt tcaaaaaagt caagtgctga
      661 cgcagcggat gactattttc cagaaaattt tagtatgtat tcaaaaatca aacgtgacaa
      721 cagctattaa ctacatcctc atccaggtct gtaacgtttg aaccactctt ctgcgggctc
      781 aaacattaag taatggaagg tacgtttcaa ttgaaacagc gtcgctgtcg tattcaactc
      841 aatttttatc acataagtga gaagcgttgc gattaaggtg aggatgactt gatttatcgc
      901 cccttgttca ctatgtgaat atagtcgttt aatggtcaga ttttgtttta agtgtttgaa
      961 caacaattca atctgccaac gggcttgata catgtcggcc acttcgtttg gcgatacatc
     1021 aaagagattg gtcaccattc gcaggatttt ttgtcccttg gtaagaaccg ttaccaacct
     1081 aaaacgctct gtcacatggt tgacaccgcc gacaagaatc acttgttggt cactgattat
     1141 cgtcccatca cgggttgttg tatctgctac ttcaatttga tccaagatgg taatttttgt
     1201 attagatttt gtccgtgtaa cgaagaaata gccgtcacga tttagtttgt ctaacaactt
     1261 gtaatcaaag tagccgcgat ccaccacata cgttgcttgc gttttatcaa caagaacttc
     1321 aaggtgatta cggtcatgct cactcgcatt tgtcatcgta aaactttcag gatactgatt
     1381 ggttttatca agataacaga gattcaaatg tagcttgatg ccagactttg ttttccgaaa
     1441 cttggcccat ggaaaccatg ctttgttcaa cggaatagtc gttgaatcaa tcaattgtag
     1501 actggttgtt tttgagcttg gctgctgagc agaaattctg gcgactaatt gcgtaaaaat
     1561 ttccatcaat acttcttgct tgatttctgg cgctctacga gataacgagc tgtaacacag
     1621 ggaatcaatg ccaagttcct tgcagaggcg ctgatctaaa aaggcgcgat caatctcgcg
     1681 atagcttggc aattcttcat aaaccgcatg aagtaacact ttaagcgtcg tttggaagtc
     1741 tagtttttta ctgtagaaat caaagtcgct aattaagctt tgagagtttt ctgataaacc
     1801 agaaaaattt attgcagaaa accatttttg aaatgaggtt tttacgttat acttatccat
     1861 gcgtgagtcc tttttattgg tcttggataa gtcatccgag aatagtataa aggacttttt
     1921 tgcgtttgga tagctttgta ttaggctttt gatgggttaa aaagtctttg caacactagt
     1981 aatttagtat ttatgtataa attgaaagat attttaccac cacttagtct tcttaaaagt
     2041 gatccaacat tttgtttgtg gcagaattta actacacact aatgcagccg aaagtacaca
     2101 actatgcagc agtttgactg cgaactggtt tccttccatc ataagtttga atgacttagt
     2161 gatggaggta cagaaaggtt gttaactttg gatgatatta aaacaataaa acatttgaga
     2221 aataatcgtg atcagtcaat tgatcagata agaaaagcaa tgaatatcaa ttggcgaaca
     2281 gcgaaaaaat acgccgatag tgatcaacta ttcattacag atcccactcg aaaaaacaaa
     2341 tccatgatgg atgatggttg gggagaaatc atcggtagtt ggctagaaga agacctacgt
     2401 ttacgcagaa aacttcgtag aacggccaaa gctatccatg aacaattgaa acctttgggt
     2461 ttccctggct cctatcgaac agtttgtgta tacatttctc aatggaaaaa gcagcgttac
     2521 gacgaaacga attaccaagc tgtcgatcgt cttgatcatc cgatcggaga agcacaggtc
     2581 gattttggaa caatggaagt tatgcatgaa gatcacatga aagatataca acttcttatc
     2641 atgtcatttc cctatagtaa tgcgacgtcc gcaagtgcca tgccagctca aaaccaagag
     2701 tgtttcctgg aagcattaaa acaactattt aatcaatgcg gcggcgttcc aaaaacgatc
     2761 agaatagata acttaactcc agcggtgaaa aaaacaaaat caaaatttga agaagcacaa
     2821 ctaaccgatg aattcttacg tttcaccatt cattatggtt gtgatgttca agtctgtaac
     2881 ccacgtagtg ggcatgaaaa aggacatgtc gaaaataaag tgggttatgt ccgatacaat
     2941 ttcttctcga cttcgccaat catggactct tttgagtccc taaataagca acttgaagaa
     3001 caattaaaag cagatcgaaa aaggacgcat tattcaaaac atcaattaat cgaagatttg
     3061 tggaagaaag aaaataaagc gttacattct cttccacaag aggattaccc aatttttaaa
     3121 gaagtggaag ctaaactaaa tcgttatcac gaaatgaaat tggatcaaac ggttattcat
     3181 attcctaaag catatggcta ttctcaattg actgttgttt tgacttggaa taaattcaaa
     3241 gttcttacgc ctcatggaga aatattaatg aacgaatatc gcccctatat gaacaaaaaa
     3301 agattgatcc catggagtga catcatgaaa caatggagta ataaattgaa tgtcgttagt
     3361 tattcgaggt attggaagta cttgcctgca cgccttcaaa attatctagc gattgataat
     3421 aatcacctga aatacagacg gataaaacaa ttactaagtt tattagcgac ctacgatatc
     3481 caacaaatta atgaacaatt ttacgaatta gtcgttcaag aagacgcccc ctctgacttt
     3541 gatgtgaatt ggtcaaacta tgatgcatta acaaaaacag gaggtgtgca aaaatgattg
     3601 gacagacgtt agaagattat tgtaaaaagc ttcgattaag ccatatcatc acgaacatac
     3661 caagagcttc tttcgaagct ccagaacaat ttattaccga tttattccgt ttagaactgg
     3721 agaccagaca gcagatcaaa ttagaacgga tggaaaaatt agccaaattt cctgtaccaa
     3781 aatcgctaaa gaactatgaa tggcacgaag atatttttct accatctcat ctagataagg
     3841 agacccttgt ctctcttgat tttctaaaaa ggcaagaaaa cattgtttgt gttggctctc
     3901 ctggtacagg gaaaacacat ctagcgatcg cattagggag aaaagcctgt agtgaaggtg
     3961 tttcaaccaa atttttccga gttaacaatc tcgtagagca attagagaat gcattaaaaa
     4021 aacaacagct agccagtttc aagaagcgat tcgaaacaac agaattaatt attcttgatg
     4081 aaatggggta cttaccattg acaaaagaag gagctgaact tctcttccaa ctgatttcag
     4141 attggtatga gcaaaagagc attattatta cctctaattt agaattcagt caatggaatc
     4201 gagtgtttat ggattctcga ttaacagctg ctctcgttga tcggctaatc catcacgctc
     4261 acattatcag cttcaaaggc gaaagtttca gattgaaaaa ttcattatct aaatcatatt
     4321 gaaggaaaaa actagttgca gcaaaactct gcactttcgg ctgcattttt ttgtaaaaag
     4381 ctattgcaca acacaaacat ttgaacgacg taattccaaa tgttggattg cttttagata
     4441 atcttttatt tattcacaat tgctaatagt ccaattttca tttttgtata gttttaatct
     4501 atactgaaaa acacttgttg ttttggacaa ctcatcaaga tactcaacac caacaatcac
     4561 ttgaatcgct ttaccctttt gtttgtaaat aggattcact aattccacaa atttcaaatt
     4621 agtattaatt ggttgcatcg cgtctttttc aacataatgc tctagttctt tttctgaggc
     4681 tgctggataa agcctgaaaa acgtctctaa aaactcggtg atttcttccg ttgtctttga
     4741 atcaattttt gaatcacttt ttacttgtct tactttatgt tttgcctttt ctggcttacc
     4801 aatcatagtg ggtaaactcg ttacaatatg attgcctttt tcatcttcaa agatcatcag
     4861 acgatatgca gaagaaattg attgtgtctt tttcccttct gtaatctttt gatctaccac
     4921 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta ctgatacaac
     4981 ctcggaacta gtgggaatat ccgctcgaac catatcttgg ctcaaagcga gcccttcttc
     5041 tgtaaggtac tgctcaagat tagtcattct cttttcaatt acctccttgt tattcttcca
     5101 tgaaaaatat tctttcacaa aatctacagt aaaattttct actccagagg tattcactat
     5161 tttctctttc acgaccactt tctcatgagt tgtatgctga tcaatggccg tgaagttttt
     5221 gtagattcca aaagcaaggc tacaagacaa taaaagccaa agggcaagca ccatttttcg
     5281 atgcttgccc acggatacta ctcgttcttt cttctttttt ggaatctttt ccttgttact
     5341 tcgctcaatt tttatcttca ttttccattc ctcttttcta ttgtttatac cgaccagcac
     5401 caataatgtg ctgttgccaa tagctatcgg taagattggt ccaacccacc ggatctcctg
     5461 catgaaacat tctgttctct cctgcataaa tgcccacatg agtcacataa gtaccggcat
     5521 cataagttga atgaaagaaa atcaaatcgc ctggtttggc atcttttaag tcaatatgag
     5581 tcatagcatc atattgatcc tgagccaccc gagggagttt caaaccaact ttggcataac
     5641 accattgagt taaaccacta caatcaaacc cagttccggg acttgaccca ccaaatacat
     5701 aagccgttcc ttcatacttg aacgcctcgt caaaaattcc ttggactgtt ttatcatcaa
     5761 actgtgttgt agttaggtac tgcttcacta ataagcaata aaacatattg ccatagttat
     5821 accgccaccc actattttcc ttaatcgcga tgggattggg ataatcgact ttttgaccac
     5881 ccgatttttc ttttgaaaaa gattcggcta aagcaaaact atgcttcttt ccgtttttcg
     5941 cgacataatc caaatacccg tcaccatagt tataagcctg aatgatacta tcttgatcgc
     6001 atcctagttc tttggaacac ttaaccaaag acgaaaaata gagacacact tgtttgatgg
     6061 atgcctcggt acttaatgaa ttcggtgata aacctgctga ttcagaactt tgcatgacat
     6121 cttccattgt acctccagat tcgacttgaa taatcgccaa aagaatcgaa acatactctg
     6181 ttatatctga ttctttggca tacttttcca ccattgaacg gtgtttcaaa actacttctg
     6241 agacactact acctccagga gaagttccag aactaccact tccctcaccg tcctcaccaa
     6301 acaacaaacc cacacacatc aagatgccca caaaagagat ggtcaggaag gttaacgaaa
     6361 taatactcaa tttttttaag ttcatctttt ctcacctggc tttggttttg ggcgagtgtt
     6421 cttttttctt tgtacttgtt gattggttcg ctttggaaaa gtgaatctct gtttcacttg
     6481 ttgaaaggaa ttgttcttgc ttagtggctg agtgcttccg tttggaatga cttcttgact
     6541 agaaagtttc cgtttgattt caggattcga atgtacttgt acccttggtt ttaaagacct
     6601 attttctggt agttcatttt tctgtggttt tatctccttt tttatcggaa cattttccct
     6661 tcctttctca tgacgattcc atgcttcgtt caatactttg cgtttttcat ccatttttgc
     6721 gtctcttcgt tgtcgttgtt tttctcgcaa ctcccctcga tttgcttttt cttcgaaagg
     6781 ccctcgctta aagtcctctg gtactttttt cgtcttttcc atgcctttat gaaggctgta
     6841 tttcaagtta gttggtgcat cggttacttg attttttgct tgtttcgctt tgtccttcac
     6901 gatttctttt gtatcgagta cttttccaac ttttctacca actaaattta ttcgtgattc
     6961 tttcttcgtg cccactggtt gttcttgttt tttcagatca gaatttagct cgttaatgct
     7021 tctcgcggaa tggttctttc tattggacac tctattgatt tttgaactac ctttcccttt
     7081 tgtagaactg gtagctagtt tcccaccaac tgctgcaccg ccaccaaaag caaaagttcg
     7141 acccacattt cgattaagct gtcgcaattt tcgattcatc aacatttgcg gtttccgcat
     7201 gactcgcctt cccattgact gactatcatt actttgaagg ttaaacatgc tcataatgtc
     7261 acctaatttg aaatagatac cggcaaaggt cacaatttgt agaaaagcta ccataaagaa
     7321 gggataggtg gccgaaatat tgaaaaacat cgtggagata ctgaatgcag tcgtaatgac
     7381 taaggtaact cccgctcgca tcataatcgt attgaacaaa cgaataatcg cccttttgcc
     7441 caaactctca tacgtcggca acatcgacaa taagaaacta atgggcagaa acatcgcaaa
     7501 gataatgaaa aggatttgtg agaacaacat aatccctgtc agaagaaaca caaaaaagga
     7561 aatcccaata ttaaacaaca ctaaaaagac cacggttcct aaacggttca tggtcttggt
     7621 aatactgaga ttcgcattgt cattatcttc aatctctgct ttgactgctt cttcccgatc
     7681 cttaccctta ttttcatccg gactcacgga taaaatctta ttaacccgac cctcgccaat
     7741 ttcttctata ttggaatcat cgaattgtaa tagtaaccag ggttgttgaa cttgaatcga
     7801 aaataaactg tctcgaatca aatccacact atcttttccc tggctttctg aattaggtac
     7861 aacaatcttg gttccaaggc taagagccgc ttcactaacg tctgagctaa agtcattgat
     7921 ttttgtaata taggtgggcg catacgcaat gaaagaaccc gagagtagaa aaatcatcac
     7981 aaaattaatg acggttcgga ctgctttggt agtttctcgt tttaataatc cggtatacgc
     8041 cacataaact cctagagcta aaatcatcaa taataggaac ccaaagtaga atcccgaagt
     8101 ctgtaatcca ttttcagtga taccggctaa agtttggata ttctttccaa tacttttagc
     8161 cgtatccgaa atgaaatcca acttgtaagc ttcttgaacc acatagcccg tggcgtttga
     8221 taaatacaga cttactgtcc aaataaaatt ggtaatggca taaagaccat acatcacgct
     8281 tttccctaag ccatcccccc aattccaagg taaccaatcc caagaactat ccacaaagaa
     8341 atccagttga taattgttca gagaatattt ggaataaagg tttccggctt caactgtatc
     8401 atcaaccaga cctgtcgcct cagctaccgt accgactaaa ctcaatagaa ctgtcagagt
     8461 gacgatgcta ataaaaacga ttcccacaat ccgaaaaagt ttctttttca tcccctcact
     8521 ccattctctc ttgaaccggc ggacgagtgt caaaagcatg gaagagttct tcaaagactg
     8581 gatgaacttg aaccacacct actcgcccat acaagtcttg catcaagcat tgaccgttt
//
LOCUS       DAYYHX010000116         8597 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000116 DAYYHX010000000
VERSION     DAYYHX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.117"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..851)
                     /locus_tag="ACHA6Y_001933"
     CDS             complement(<1..851)
                     /locus_tag="ACHA6Y_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8988018.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTI"
     gene            997..1800
                     /locus_tag="ACHA6Y_001934"
     CDS             997..1800
                     /locus_tag="ACHA6Y_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM8988019.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1978..2925
                     /locus_tag="ACHA6Y_001935"
     CDS             1978..2925
                     /locus_tag="ACHA6Y_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM8988020.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2993..3286)
                     /locus_tag="ACHA6Y_001936"
     CDS             complement(2993..3286)
                     /locus_tag="ACHA6Y_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM8988021.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3314..3979)
                     /locus_tag="ACHA6Y_001937"
     CDS             complement(3314..3979)
                     /locus_tag="ACHA6Y_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM8988022.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4119..4751)
                     /locus_tag="ACHA6Y_001938"
     CDS             complement(4119..4751)
                     /locus_tag="ACHA6Y_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8988023.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4758..5825)
                     /gene="liaS"
                     /locus_tag="ACHA6Y_001939"
     CDS             complement(4758..5825)
                     /gene="liaS"
                     /locus_tag="ACHA6Y_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM8988024.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(5822..6553)
                     /gene="liaF"
                     /locus_tag="ACHA6Y_001940"
     CDS             complement(5822..6553)
                     /gene="liaF"
                     /locus_tag="ACHA6Y_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM8988025.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(6722..7201)
                     /gene="greA"
                     /locus_tag="ACHA6Y_001941"
     CDS             complement(6722..7201)
                     /gene="greA"
                     /locus_tag="ACHA6Y_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM8988026.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7337..8512)
                     /gene="mltG"
                     /locus_tag="ACHA6Y_001942"
     CDS             complement(7337..8512)
                     /gene="mltG"
                     /locus_tag="ACHA6Y_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM8988027.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         2553 a         1765 c         1335 g         2944 t
ORIGIN      
        1 tggatcgtca catgtgtaat accatatttt aattttaata aagaaattat acgaggatat
       61 atcttttcaa tctcgcttag tagctgatct tcaaaatcaa tatgtccgtc gaaaataatt
      121 atcttttcgc tatactgcca aatatggaca tgatgaacat cctttacccc atcgattgct
      181 tcaatttctt gttccatttt ttcatagtcg atagcaggtg cactttgtat caaaatagcg
      241 gcagcttttt tgatcaccat tatcgcttct atcaatatat atatcgaaat gatcagtgta
      301 ataactggat cgatccagta gatatcgaaa aaacggatca aaattgctcc gataatcaca
      361 cttaccgaag acagtgtatc acttaacaaa tgcaaataac tagattttat attcaaactt
      421 tcatctgctt cttgtcgtaa taataatact gagaacaaat tcgcgagcaa tccaataact
      481 gctactgtta gcatcaaatg actatttatc ttttctggtg aagtgaaccg cttgaatgct
      541 tcaacggtta aaaacaagga taatatgatc aatatgaatg agttgatgaa agcactcaat
      601 atttctaaac gctggtagcc atacgtacgt tttttatttg cttcttttcg ggccattttt
      661 tgtgccccgt aagaaaaaat aattgccaat gtatcactaa ggttatggaa agcatctgaa
      721 acaagagaca aactccctga cataatccct ccaataaatt ctgataaagt gatcaccgca
      781 ttcaaaacca ctactccaaa tagttttttt gatgatctct gtggctgtgt aggcactatt
      841 ccttcctcca ttttatttat attggatagt ctttagacaa gaataacatt tttttgatat
      901 ttcaccaatc attcagctgt ttccttaaag cacttgtatt tcacttgata cttcgctaga
      961 attgcaagtg atgacactac tagatgagga gtacttatga caacaaaaca attaattatc
     1021 agttccttta tacgattcaa agacctaaag gaggcgagaa atgtcccatt ttggaaagcc
     1081 attgtctatc ttttagtttt gagtataatt atggctttgc caatctcttt tcaagttttt
     1141 caagtcttaa acaacattca attggatggg caaaagattg ctactcgcat tcctgatttt
     1201 acaatccaaa acgggcagct tcaaaccgaa gaaaaagaag gattcattta ccaaacaaac
     1261 tccattatct tcacatttga tccagaggga aaacgaacag aacaggacat ctctactgat
     1321 ttgatgggga attttgttag tgtcgggatg ttaaaaaaca aacttatcat agctcttcct
     1381 aatacaggca cgacttctgc tttgttgaac aataaccaat tagaacttcc ctacaccaat
     1441 gaatctttaa aaaacttaac aggtaagcag ctccgttctt ttttaagtga agcatctata
     1501 ccactttgga taaaagtcct gacatttata ttttctattt atcctagttt tctgaatctg
     1561 ctgatcactt tgctatttac caacattgct gcttttctct atgcacgctt tcgactaaca
     1621 aaagctactt ttcttgattg tttaaaaaca ttgatctatt caatttcatt gcctactata
     1681 attgcaacaa tcttgatggt ctttcttcct tcttttgaca ctagcgcctt cattgctttt
     1741 gcaggaatct ttatttttgc tcaagctgtc aaaggatggg caaaaatatc gatcaattaa
     1801 gaaataaaga caactcgtta tttctacttc aatagaccct tttagaggtt gtgacatttt
     1861 tttgtcacag cccctttttt atttgaaaaa tatttctttc tcaaaaaaca agcaaggcgc
     1921 ttcaaagttc ttcttgtatg cgctacactt agagtaaaga aaggcgggaa attaactatg
     1981 tcagaaagaa tcatttatgc agatcgtcca tttcgtgatg aatttatcga agaaatccat
     2041 actatagcgc ctggttatat ctttaaaaca gaattacaac cagaggatct acaaaatgta
     2101 gagatcagtt tgggctggaa ctcagactac caagaaaaac tattagcctc ttctaccttg
     2161 aaatgggtcc aatctatctc agctggtgtt gatcagctac cccttgagac tttttcaaag
     2221 aaacatattt tactttcaaa tggtagtggc atccacagtg aatcgattac ggagcatgtc
     2281 ttaggaatca tccttgggta ttctcgtggt ctttttcaag cacaaaaggc acagagtgac
     2341 atgaaatggt taggttcatc ggtccattac caatctatcc aagggaaaaa aatactaatc
     2401 attggaactg gccatattgg aaaaatgatt gcaaaaaaat gcaaagcatt agacatgaac
     2461 tgctatggaa tcaatacatc cggtcatcca gcagaaggaa tggaaaagac tttttcttta
     2521 aatcaactaa aggaagttct tcctgaagta gatttcgtag tcaatatctt acctctaacg
     2581 gaggaaacaa ctgggctctt tgatcagcat atgtttgaac actttgatcc aaaatctgta
     2641 ttcatcaatg ttggccgtgg tgcttctgtt aagacacaag atctggtgca ggctttaaat
     2701 aaccagcaac tttcctttgc agcattagac gtttttgagg aagagcctct tccacaagat
     2761 catcctcttt ggaaaatgga caatgtactg attacttccc acattgcagg tttgacacct
     2821 gattttcaaa agaaattaat ggctattttt ttaaccaatc tgaaaagcta ctttgccacc
     2881 catgagcttc aaaccaatca agtaaaattg tctgcaggat attaatagct gaaaacaata
     2941 ttttcaaaag aaaaaagaga aggtgacaaa aattgtgtca ccctctcttt ttttattgtt
     3001 ttttaaagtg ataggttggc tcctccaggt tgggatcata atcaacaagt aaatcatatc
     3061 ctttaaagaa ccattcatct gcttcctcga taaaaaaaag tgtcccatca atttccactt
     3121 tctttacagg atgttctggc tgttcaactg acattccaat cgaaaaacca tcatgaacct
     3181 ctgttttgcc gtagactttt ccaaaaaaac gtattccgta agtttctggc agatcaattt
     3241 ctgagtcaaa ccacttttgt gcttttggcg ttaccgttaa tttcatccca acaacctact
     3301 ttctcttttc tttctattga ttcatcttat cgtctagaat atccacatcg cctgtctctc
     3361 caataaccag tagattatca ttttcacgaa catattcatc tgctgcagga gaagcgataa
     3421 ccgtttgatc tactcttcgt atggcgacta ctgttaagtc aaaacgttgc ctgaaattta
     3481 attcagctaa tgttttgttg aaaaatttag gattggagac tcggatttct gctaaagaat
     3541 atttatctga caactctagg taatctaaaa tattctttga cactaaatta tgcgcaatcc
     3601 ttattcccat gtctcgctcg ggatggacga cgcgatcagc tccgatcttt tcaagtactc
     3661 tggcatgata ctcattttgg gcttttgcta aaatatttgg tacgcccatt tcctttacca
     3721 tcagagtaac taagatactg gcttgaatat cttctccaat agcgacaatt acatgatcga
     3781 aatttcttat gcctaatgat cgaagtgtca tttcatcctg tgcatttgcc acaacagcat
     3841 gagtggctat attcatatat tcattgaccc gatcttcatt actatcaata gccagtactt
     3901 cttgaccagc ttcgatcagt gttcggcaga tacttccgcc aaatcgtcca agacctacaa
     3961 ttgcaaaatt ttgtttcacc gttttcctcc tttagtatct tcttttctca gtataacaaa
     4021 gcctttacaa aaacaaaagc aagcatcatt attgtaaaaa agccaagaaa aataatagtt
     4081 attttctcag cttttgatta tcctctttat catcgttttt atttcaccaa accatgtttg
     4141 aatgcataga tagcggcttg tgtacggtct tccacttcta gcttagccaa tatattggaa
     4201 acatgtgttt ttactgtttt cagtgtaata aacaattcgt ccgctatttc ctgattactt
     4261 tttccttcag aaatcagcat taaaatttca ttttcccgat tcgttagttc ttcatgtaat
     4321 acaggctcat ttcgccgact cattttttcc atcatttttg ttgtcacttc tggttctaat
     4381 actcgctcgc cacgttgcgt tgcccttata gcatcagcaa tttcatgtgc ggtagaggtc
     4441 ttcaacaaat accctgctgc acctgcttca atggcaggat aaactttttc gtcatcaata
     4501 aaactggtta cgataatgat tttagcttct ggccaatctt ttaagattgc ttttgttgat
     4561 tcgataccat ccatttcttc catgactaag tccattaaaa tgacgtccgg tcgaagtgtc
     4621 atcgccttct catagccttg tcgtccattt tcagcttctc caattacttc aatgtcttct
     4681 tgaatagata aataaaaaga gacgcctaag cgcaccattt catggtcatc tactaataaa
     4741 acttttatca tccttatcta tgcctccttc atcaaaggga ccttgatttc tacacttgta
     4801 ccttgacctt taaaactaat gatttttact gttccaccaa taccagctac tcgttctttg
     4861 atattgttca atccataact tcccgttttc gtttcattca tattaaaccc tgttccgtcg
     4921 tcaataatac gtaacaaaag attattgtct attttgtgca aatatacctc taattcattg
     4981 gctttagcat gtcttaatgt atttgataac aattcttgaa cgattcggaa caagtgatcc
     5041 tcaatggaac tagatagttt tacatcttct acatcccatt tcagcgaaat ctggatttta
     5101 ttttgcaatt ccttcaataa ctgttcgata ccttgcttta aacttttctc ttctaaattg
     5161 actggacgta gatgaagaag cagcgcacgc atctcagact gggaagcgtt gatgatctct
     5221 gctaccattt tcaactgctt gcgaaacatt tctggtgtct cgcttttttc tgcttgctcc
     5281 gtcaatgccg acatcatcat cattgctgca aacaattgtt gcgaaactga atcatgcagc
     5341 tcacgggcta atcgatgtct ttctaattct aaaatttctt ctttggtttg tccatctaca
     5401 tattggggac ggctcgttac aatttgtaat tcactagata cttcaatcat tttttctttt
     5461 atcttagtga tttccttatc aatatctttg atgtataaat cgtccgatgc acttgggatc
     5521 ggaagaagca acaatttgga ttcataattg ccagaagaaa gtgcacgcag cttttcttcg
     5581 attttcccgt attttgtctt ttgaataagt gataataata gaaagacaat taatcctaca
     5641 cctaaggaaa tggctaacaa atggaagata agcggaacat ataataaacg tgcacgcagt
     5701 aattctcccc accaatgact ttgattgctg gcataaaaat aagtgaaaag tgaaaataga
     5761 ataataagga aagccgcaat ccccgaataa acagctagca tcgctctgga tatttttccc
     5821 atcatattcg tatcacctcg acatctccaa tcaaggtatt agtaataatc ttcaatctac
     5881 gagggttttc atcataatca ctgctgtaga ttttgatttg ttcatttttc aaagaaaatt
     5941 gctcttcttc aaacaagaca tttcctacta atgtcgcatg ctctaatcgg atggctacgc
     6001 ccaaaggtac taagatccgt gttctgccta tcccttttct tacaatcaca atattatcat
     6061 cttttggcaa aagcgtattg cccaaatcta tgatagtatc accagaaatg acggcgatgt
     6121 tgatatcgtc ccattcatag acgtcgtttc caatacgttg gttaccgaat aattgctgtc
     6181 ttttccgctc attattatgt gtttttaatt gctctgtttg gatcatcatg atctgtttct
     6241 ttcgccaaaa agcatttttg gttaaatcta tccctgaaat ctctacacct ttcagaccaa
     6301 taaataaaac ggcaaataca gccatcatcc ataaagcagg actgttgact aagctaaaaa
     6361 aaatgaccaa acttcctaat atcaattgaa aattttgaaa tttagtacga ggatgtttgc
     6421 gcatagctag atatatattg aaaataccga atatcactaa aagcaaaagt ccagtattgt
     6481 ttacgatttg ccaaacagca aataacagca gtagtgcttc aactaccaca aaaaaacgcc
     6541 aagaactatt catcgctctt tctccctcct gacacattct catttcatca tacctatttt
     6601 tttaactgtt ggctatagga ctttagatgt aagatcgttt gttgaaatct aaaatacaca
     6661 aagagaaaaa gatctagagt gtatcatcag taagatgaac tctctagatc tttcagtcag
     6721 actacgcttg ttctactttg acgattttaa ctaacatatc gccacctgga gtagcaatag
     6781 ttacttcatc atttttacgt ttgccaatca atgcttgcgc aataggagaa tcattggaaa
     6841 ttttgccaga aaacggatct gcttcagcgc ttccgacaat cgtgtactct tcttcttctc
     6901 catctggtag ttcgatgaat gtaactgttt taccaattga tacctcatct gcatctacac
     6961 cgtcgttatc gatgatttga gcaaaacgaa tcatattttc aattgttgtg attcttcctt
     7021 caacaaatgc ttgttcatct ttcgctgatt catattcaga gttttcggaa agatctccga
     7081 aaccgcgggc aattttgatt cgttcaatga tttctccgcg tttgactgtt tttagttctt
     7141 ctaattcttt ttctaatttt tctttacctt ctagagtcat tggataaact ttctctacca
     7201 tgtgtaacgt ctcctttttt cgataaaata ccgcaaaagg taatcttttg aggaatatag
     7261 tccaaaaaag attaccacgc ttttcttcaa atgtaaatgt ttttctaaaa atgatcaatg
     7321 tctagagcct gtttttctaa gaattattta catatttttc cactaattcc atatgttctt
     7381 cataagtttt agcgaaataa acatttccag tgctcgtatc tgctacgaaa taataatagt
     7441 cattttctgc tggttctaat actgccttga ttgcctcttc gcttggattg tcaaaaggtc
     7501 caggtccata gccggtattg gtatacaaat tatatggtga atctacagct gtatcttcat
     7561 acgtcactaa ttctttgtgt tcacctaatg cgtataaaac agagatatct gactgaagtg
     7621 gcatatttgc tttgattcgg ttaaagaaga cttgtgcaat gtttttacga tcattttctt
     7681 ttacgccttc tttttcaact agagaagcta gagttaaaac ttcttgtaca gtcaaatttt
     7741 tctgcttgat agctgaataa tagttttgca tgaccgtatt cgtcttgttc accatttcaa
     7801 tcactagatc cttaagcgaa gtactcgtgt aataatcata tgtggctggg aataagtaac
     7861 cttccaaacg gtattttgtg ttctcagctt tagatgcact ttccaacagt tctgggaatt
     7921 tttggtgcaa ctcgttaaag aaagtctcat cattcactaa atctaaaaat tctttcttgt
     7981 ctttaccagt taccttcgct acccgttcag caatctgatc gatatcgtac ccttctggaa
     8041 tggcaatttt cccatctgca acttttgttg gctcagaagt accgccttct tgcaactgtt
     8101 tgccaatctc gtcaagagtc atattaggcg ctaactgata atagccagct tgaaagccag
     8161 ttaaattttt aaattttgta taataattga aaacaatccc gcttttaata atattgtcct
     8221 tctccaaaat ctcaccaatc tgcttgttag aagagccact cgggatctcg acttggacta
     8281 attggtcatc tgatttatct aaaggcttca acccgctatc tacataacga tacacggtaa
     8341 atccaagaaa tccgccaata atcaataatg taagagcaat cactaaaatg atcttcctca
     8401 caatcttatc ttctttttta cgcagatttc tccgcttgtc gattatatct gctgtagctg
     8461 ccttactggc ttctttttta tctttacttg ggtgttgttt gtttcctgac aaaacaactc
     8521 ctccttacta tcaagttcgt aactttattc attatacatg atatttgaat tggcgtgaat
     8581 gattttattt aaatatt
//
LOCUS       DAYYHX010000117         8539 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000117 DAYYHX010000000
VERSION     DAYYHX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.118"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..299
                     /locus_tag="ACHA6Y_001943"
     CDS             99..299
                     /locus_tag="ACHA6Y_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8988028.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            446..3235
                     /locus_tag="ACHA6Y_001944"
     CDS             446..3235
                     /locus_tag="ACHA6Y_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM8988029.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            3282..3809
                     /locus_tag="ACHA6Y_001945"
     CDS             3282..3809
                     /locus_tag="ACHA6Y_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM8988030.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            3830..5020
                     /locus_tag="ACHA6Y_001946"
     CDS             3830..5020
                     /locus_tag="ACHA6Y_001946"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8988031.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5060..6358
                     /gene="asnS"
                     /locus_tag="ACHA6Y_001947"
     CDS             5060..6358
                     /gene="asnS"
                     /locus_tag="ACHA6Y_001947"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM8988032.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7534..8208
                     /locus_tag="ACHA6Y_001948"
     CDS             7534..8208
                     /locus_tag="ACHA6Y_001948"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8988033.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8205..>8539
                     /locus_tag="ACHA6Y_001949"
     CDS             8205..>8539
                     /locus_tag="ACHA6Y_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988034.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFA"
BASE COUNT         2881 a         1545 c         1847 g         2266 t
ORIGIN      
        1 ggtaatctgt ggtagcttgt ttcaaataaa catcttccag atatacacca ttcaagtaat
       61 tgcaaaatat gtgttgatac acaaggagga tttttattat ggaaacaggt acagtaaaat
      121 ggtttaactc agaaaaagga tttggattca tcactgcaga aaacggaaac gatgtattcg
      181 ttcatttctc agctatccaa ggcgacggat tcaaaacttt agaagaaggc caagcagtaa
      241 ctttcgatgt tgaagaaggc caacgcggac ctcaagcaac aaacgttact aaagcttaat
      301 taacctatac taaaaaataa acgcccagac atttgcgtgt ctgggcgttt ttgtctattt
      361 tctttaaaaa gcgatgagag tatttttctg cactttgtag tataatttca aaagaacatt
      421 agaggcagga gaagaggaag atattttgaa accaagaact tatgcagtcg ttgatatcga
      481 aacgactggg acgaatccaa aagaagatcg gatcatccaa tttggctgcg tcatgattga
      541 atcaggtcgg atcacttcac gtttttcgat cgatattcat ccagggcgga agatttcaaa
      601 acaaatccag catttaacag ggatcacgaa tcaacgcgtc caaaaagcgc cttatttcga
      661 agatgtcgcc caaacgattt acaatctttt ggcagacacc atatttgtcg cgcataatat
      721 ttattttgat tataattttt tgaaccatga actcatgcga tgcggacttc ctagtttgaa
      781 gataccaggc atcgatacag tagaacttgc acaaattttc ttgccgacgg aaccaagctt
      841 tcgcttagct gatttgtcag aaagccttgg atttattcat gagaatcctc atcaggcaga
      901 tagtgatgcc gaagtgacag gtcaacttct tttgctgatc gaagaacgga tgagaaaatt
      961 gccaatcatc acgttagagc agattgcgcg attaagtaga cacacaggta tggatacgag
     1021 tcgtttcatt tatcatgtga ttgaagaaat gaaagaaaaa ccagagccac ttgatccctc
     1081 attagaaatc gtcgatgggc tagctttgca gaaaaaagaa gtggaactat tcacatccgt
     1141 ccattatggc gagcggacat atccaagaaa aaaacaagca aaagaaaaaa tgtttggtaa
     1201 aacactgatg tatcgaaaag aacagaacag actgatgaat gcggtctacg accatttcac
     1261 aaaagatgaa tcaaaagatt tgatgatcga agcagctaca ggaatgggta aaacaatcgg
     1321 ttatctgctg ccattgagtt atttggctac acctgaaaaa cccgctgtca tcagtaccgt
     1381 ttcgctagtt ttgcagcagc agatcataga aaaagacatt ccattgctga atcaattgct
     1441 agatcagccc atacaacctg taattatcaa aagctatcgc cactatattg atttgcagcg
     1501 ttttaaaggc acacttgtgg aaccgccaga acaaaaacaa tatgctttgt atcaaatggc
     1561 ggtcttagtt tggctgacgc aaacgaagac tggtgatttg gatgaattac atttaacaaa
     1621 tttgaaccat tcattctttg cagatatcgc gcacagaggt attggatttt tagcaagaaa
     1681 tcagtcattt tacgaacaag actttgttcg tttcttgcaa aagaaaatca ggcaaagtaa
     1741 ttttttgatt gtcaatcatg cgtttttagt acaagaaaca cagcgaaaag aaccattgct
     1801 tccagctagt ccatttttgt tgatcgatga agtacatcat ttgccggaaa tcgctgaaaa
     1861 agtaaatgac aaacgattaa gtattaacta tttccataaa caaatccagc aattagaaga
     1921 accaaatcag ctgtttgacc gaatacaaag ctatttaccg aaagaacatg aagccttacg
     1981 ctggttatcg atttatcgag aagaactttc tgcgctatgc gaagcacaga tttgtatggc
     2041 agaaggatta atgagtttag tgagcggcaa gcaacctaac ggacagtatc cagaagaagt
     2101 catgatgaca aaagagatgt tggatcagct gccactggaa tctgggacag ccattgatca
     2161 cctgcagctt ttttatcagg agatccagca gctacagtca caaatgcgtc tgacttttga
     2221 gattatccaa gaaagctgga caaaccagat gcggatcgat ttagcaagtt tatatagcct
     2281 atttgatcaa ttagacaagc aatacttgct gatggatcaa tggctggaag actggcgaga
     2341 tcatttgatc cattggttcg ttcctgggaa cacagcgcag cagatgattt ttcatctcca
     2401 tgattttcaa gcggctatgt tgccgtcgac tgtttggtat cctcgatatg atcgtattct
     2461 ttatattgga ggaacgctga agatcggccc taatcgacac tatctctcta agaggcttgg
     2521 cattgaagca gcacctctta aagtgattgc ctctccttat gattatgaga aacaggcaag
     2581 gatctatgtg ccaaaagagg cacctgccgt ccatcaagtc agcaatcaag catatatcga
     2641 ttatttagag acagcactta caggtctgat cgaacaagaa gatcggccaa tacttgtgtt
     2701 attcacttct catgatgttt tgcagaaggt ctatcagaaa atgcatctgt ctatgctaga
     2761 aaaaggacga gaaatactag ctcaagggtt tggtggaagc cgggagaagc ttttgaaacg
     2821 cttcattttg tcagaaaaaa gcgtattgtt tggcgctgac agcttttggg aaggtgtaga
     2881 tttgcctgga gatgctttgc agatcgttgt agtcacacgt ttacccttcg ataatccgct
     2941 tcgcccgatg gtcaaagcta ggaacgctta tttagaatca gaggggatca atccttttta
     3001 ccaggaatcc gtacctaaag cagctttgaa gttacgccaa gcattaggtc gcttgataag
     3061 gacggagaca gatcgtggcg tattgcttat tttggatcga agactagtca ctgcaaaata
     3121 tggcgcacgg atcatccatg cattaccgaa aaaagtaccg atcaaagagc tagctatgga
     3181 ggaaatctat caggatatcc atgaattctt aaagaaaaat gaagaaggaa gttagaggag
     3241 ctgtcttttt tcataattaa aatctgctat aatgaaaagg aatgaagata aaggagcgac
     3301 gacccttgaa gaaaaaacag acaaaggaaa aaacgattaa tcgcgtgtta cttggcttga
     3361 tcgtattttt attagcggtc atcgtgatca gctcgatttt ttacttgcgt tccagtcgtc
     3421 cggcggtgca ggcgaagaaa gaagcagtag aaatcgcaaa gaaatatgca aatcttgagg
     3481 aaaccgatca tttttattgg tttactcgaa agaagacata cttttcactg acaggtacaa
     3541 atgataaagg aaaagaaatc gccgttatca ttcctaaatc tggtgaaaaa gtaaaagtat
     3601 tggatcaaac aaagggactg actgaaaacg aagcaaaaca gacaattgct gcggcccatc
     3661 ctgaaatcca aatcgaaaaa gctgctttag ggatgtatga tgatcagcct gtgtgggaag
     3721 tagtaggaaa agaaacaaat gggaaactga attattacct gattggcttt gaatctggag
     3781 atgaagtcaa gacaatcaag ggaatttaaa aggtaaagga atgaatggga tggagttatc
     3841 aaaaagaaca aaacgattag aaccgtctgt cactttggca gctgcggcaa aggccaaggc
     3901 attaaaagca aaaggcagag atgtgctgag tttgactgtg ggagaaccgg attttgcgac
     3961 accagaaaac atccaagaag cagcaatcga agcaatccgc aatgggaaag ctagctatta
     4021 tacaccaaca gcaggaatcc cagagcttcg acaggcaatc gttgattatc tgaagaaaaa
     4081 ctatggactt gtctatgaac cttcacaaac agtcgtaaca gacggagcga aatttgcttt
     4141 gtataccctt ttccaaacga tccttgaccc gcaagatgaa gtcatcattc ctgtgcctta
     4201 ctgggtcagt tacggtgagc aagtaaaact ggcagaagga cttcctgtct ttgtgaaagg
     4261 ggaagaatcc aatagctgga aagttactgt tgaacagcta gaacaagcac gttcagaaaa
     4321 aacaaaagcc gtaatcatca attctccatc gaatccgact ggaatgattt atagcaagaa
     4381 tgaactgcag gcaatcggtg aatgggcagt aaaaaatgat attttgatag ttgctgatga
     4441 tatttatggc cgtttagttt ataatggcaa tgagtttact ccaattgcaa cgatttcaga
     4501 agcaatcaaa aatcagacca tcatcatcaa cggcgtgtct aaaacatatg cgatgacagg
     4561 ctggcgcatt ggttatgcgg tgggaaatcc tgtgatcatc aatggaatga tcgctgttgc
     4621 ttcccaatca actagcaatc cgacagcagt cagtcaatat gcagctgttg aagcattgaa
     4681 aggtgagcaa gatacagtag aagaaatgcg gaacgccttt gaagaacgcc tgaatacgtt
     4741 atatccgctt gtggcagaac tgccgggtgt ttctcttgaa aagccgcaag gagctttcta
     4801 tctttttcct aatgtaaaag aaacgctacg catgtgtaag tatgaaaatg tcacacaatg
     4861 ggtagaagat cttttagaag aaacaggggt agcgcttgtg actggcgaag gctttggggc
     4921 accagaaaat gtccgcatga gttatgccac tgatcgcgct acgttagaag aagcggtacg
     4981 cagaatcaag caatttattg agagtaaaag tcaaaattaa tcctgaagaa attcaaggaa
     5041 tataaaagga gagtcatacg tggaacaaat tcaaatcatc gattcgaaaa accatgtggg
     5101 agaaacagta acaatcggcg cttgggtcgc aaataagcga tcaagcggaa aaatcgcatt
     5161 tttacaatta agagacggaa cagccttttt ccaaggtgta gttgtaaaaa gcgaagtgtc
     5221 ggaagaagtt ttccaaacag ctaaaaatct acaccaagaa acatccgtgt tagtaactgg
     5281 agaaatccgt gaagacagtc gttcaaaatt tggttatgaa atcggaatta caaatgtgga
     5341 agtggtaagc gagagccacg attatccgat cacaccaaaa gaacacggaa cagatttctt
     5401 gatggatcat cgtcatttat ggctgcgttc ttcaagacaa catgcgatta tgcagatcag
     5461 aaatgaaatc attcgtgcaa cgtatgaatt ctttaatcaa catcattttg tcaaaatcga
     5521 tccgccgatc ttgactggtt ctgcaccaga aggcactacg gatctattcg aaacaaatta
     5581 ctttgatcaa aaagcttatt tatctcaaag tggccaatta tacatggaag ctgctgctat
     5641 ggctttcgga aaagtctttt cattcggtcc gactttccgt gctgaaaaat caaaaacacg
     5701 acgtcatttg atcgaattct ggatgatcga accagaaatg gctttcactc atcaagaaga
     5761 aagtttagaa gtacaggaac aatatgtagc attcttagta caaaatgttt tagatcattg
     5821 cgaatatgca ttagatgtac taggacgaga caaagaagtc ttaaaaactt atacaaaatt
     5881 gccattccct cgtattactt acgatgatgc aatcgaatta ctgaagaaaa atggatttga
     5941 tgacatcgaa tggggcgaag actttggttc acctcatgaa acgttcattg cgaattcatt
     6001 cgaacagcct gtctttatcc taaactatcc aaaagccatc aagccattct acatgaaacc
     6061 acatccaaca agagaagacg tggtaatttg tgcagatatg atcgcaccag aaggttacgg
     6121 tgaaattatt ggcggaagcg aacgggcaac agactatgat ttcttgctag aacaaatccg
     6181 taatcacggt ttggatgaaa aagaatattc atggtatctt gatctacgta aatacggttc
     6241 tgttccgcac tctggttttg gtctaggcct tgaacgaaca gtcacttgga ttgctggtat
     6301 cgaccatgta cgtgaagcta ttccattccc acgtttgttg aaccgaattt atccataaga
     6361 ggtgatgaaa aagctgttct gcatcaagta attagaacag ccaagcaaaa acagctgttc
     6421 atgtttttga attggcggga cttattaccg cagatgtaag cttttgaata ccgtttagtg
     6481 gagaggttgt gaaaaagctg tttagtttcg agtattaaga aaaattttgt ggaaatggct
     6541 ttccacattt ttgcataaat ttggcttaat accgagaaac tggcttttga acaccgttta
     6601 aggaagaggt tgtgaaatca gtgccttgac ctgagtatta gagaaacaaa acggaaaaat
     6661 agcttctcat attttaccgt ttttttagct aatatcgagg gtcagctgat tgaacaccgt
     6721 ttatatcaag aggttgtgcg cctgccgttc agctccgagt cacaaggagt aatttcaaaa
     6781 aacagttttt catgtttgat gactattgtg acttgtgacc gaggagttgg ccggagagca
     6841 acgtttattt aaaaaaaaga actgcgaact ctcgcagttc tttttttaaa atacgggata
     6901 aaggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg attcacaagg
     6961 ttctataacg aaatcgcaat caatcaaaca tatacaaaga aggttattca aatggaaatc
     7021 tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagtacctga aaacaaacta
     7081 atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaatgat ccatatgtaa
     7141 gtttaattca tccttgaaat catcggacca gataaagtta tgaccaatct atcccaagca
     7201 atctagaaga gatattagta aaagaacatt tactggaagt cttagatgag cctataaata
     7261 tatgttctga tggcgtcatc agtggtctga ttgacggaag cttaggtgtg atcgatttct
     7321 ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa aagtataaaa
     7381 aactagacac ttgatgagcg ttcattcaat tgattgaagg gactaggctg gtgtcatttt
     7441 ttatacatat attttctctt tcgtcttgat agggcttatg ggataatata aggaagaaaa
     7501 tcatcgtttt attcaaaaag gagtttttga caaatgaaaa aacgtatatt atttatcggt
     7561 gacagcatta cagacgcagg aagagaccga gcagaagcaa ctgatctagg taaaggttat
     7621 ccacttttga ttgccggaaa actaatctcc caatatccaa cgatgaaatt tgaatgcatg
     7681 aaccgtggga taggaggaga caaaattgca gatctgcaag tacgctggca agaagattgt
     7741 ttagctttac aacctgacta tgtatcgatt ttgatcggaa tcaatgatac ctggcataat
     7801 actagcgatc aggctatatt cggaactgag aaatctgcgc agcaatttga atccgtttat
     7861 cgaaaattac taaatgaact tcaaacagcg gaaatctgca acatcatctt gatggagccg
     7921 tttgtacttc ctataaccga cgaccgtcat ggctggcgta aagacttaga ccctaaaatc
     7981 caaattgttc gtaaacttgc taaggaatat ggcgcattgc tggttccttt agatggcata
     8041 ttcaatgctt tagggattgc ttatggtttt tcgaaatata caagagaaga tggggtccac
     8101 ccaacgattg gcggacatga gatcattgca caaagctgga tagatgcaat tgccgcagtc
     8161 gattttttta gtggagataa aaatgagcga aagaaaagtg agaaatgatg gaaatacggt
     8221 gggcagtaaa agaagattta cctcaaatca atcagctgat tactgatcat ccagaatcat
     8281 tcgtaaaagc aaaaacggca gctgacctaa atgataagaa taaactattg ctcgttgctg
     8341 taacaaaaga aaaacggatt atcggatttg cgcaagcgga cgtgcaacag tcagaagaac
     8401 gggtggatca gcgaccatca ggaaaactaa tccaacacac attagtaaca aaagaaaata
     8461 cggaagaaat ccaaaaaaaa tttgcagaat acttgatgct ggaagctaaa aaaagaagaa
     8521 taaaatcaat ttttgccca
//
LOCUS       DAYYHX010000118         8470 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000118 DAYYHX010000000
VERSION     DAYYHX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.119"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..290
                     /locus_tag="ACHA6Y_001950"
     CDS             3..290
                     /locus_tag="ACHA6Y_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988035.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            706..2244
                     /locus_tag="ACHA6Y_001951"
     CDS             706..2244
                     /locus_tag="ACHA6Y_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM8988036.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            2241..3206
                     /locus_tag="ACHA6Y_001952"
     CDS             2241..3206
                     /locus_tag="ACHA6Y_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8988037.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3193..4515
                     /locus_tag="ACHA6Y_001953"
     CDS             3193..4515
                     /locus_tag="ACHA6Y_001953"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8988038.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4578..4682
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4756..5499
                     /locus_tag="ACHA6Y_001954"
     CDS             4756..5499
                     /locus_tag="ACHA6Y_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8988039.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5514..6506
                     /locus_tag="ACHA6Y_001955"
     CDS             5514..6506
                     /locus_tag="ACHA6Y_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8988040.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6518..7255
                     /locus_tag="ACHA6Y_001956"
     CDS             6518..7255
                     /locus_tag="ACHA6Y_001956"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8988041.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            7245..7967
                     /locus_tag="ACHA6Y_001957"
     CDS             7245..7967
                     /locus_tag="ACHA6Y_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8988042.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8051..>8470)
                     /locus_tag="ACHA6Y_001958"
     CDS             complement(8051..>8470)
                     /locus_tag="ACHA6Y_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8988043.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2734 a         1460 c         1827 g         2449 t
ORIGIN      
        1 aaatgcgagc agcagactta ttgacaattc ttacaaaatc acccaaaaaa acagagtatc
       61 aaggagtttt tttggctcaa gaacaaaaac tggtgcctgt gacaatggtc cagataaata
      121 aagcaaatca atttatcttt ttatttgaac acggcaaagc accacttgct atgaaagaca
      181 tctttctttt tttgatgaag aaccgtgaga aagctgttta ttatcagtca aaagaagaac
      241 tcattcctct ttatggagtg aaagagatag aaaataaagt ggtgatataa gtaaaaataa
      301 gaaaacaaga cgtttatgat gtcaaaacaa ccatttttta catttttggc aaaaaagtgt
      361 catgatacag tataatattc gttgaacagg ccaataaagg tgttctcccc cacgagattt
      421 ctactttatt ggctgtttct ttttattaaa ataaaagttg tgtgtgtaaa aaagatatag
      481 ctgattccaa gtgacaattt cgtatagatt tttatggtat tcgtagtgaa aaaacgagaa
      541 ccaaaaagtt gatccgactt tgttgcttgg attctttttt tgtcagcata tttagaggta
      601 tttttttctt ttttttgttc gtgctgtaag gttaaggcaa gacaagttag ttgggaaaaa
      661 ggaaagaaat gtttatactt aaagaataac agaaaggaag gtcgcatgaa taattttata
      721 caaacattaa gtgaaagaaa aggcgagttg ctggaagctg catttcaaca tctgtccatt
      781 tctctcattt ctttgctgat tgcagcactg attgctattc ctttagcgat ttgggcagcc
      841 aatcataaaa aaatggcaga agtcttattg cagatcacgg gtgtgctcca aacgattcct
      901 tcacttgcct tattaggttt acttatccca tttgtaggga tcggaactgt tccagcattg
      961 attgcacttg ttatttacgc tttgcttccg attttccaaa acacgtatat tggtctagct
     1021 gaaattgacc cgtctattga agaagcagcg gtagcatttg gtatgtccag aatgagacgc
     1081 ttgctgaaag tagagcttcc catcgcatta cctgtcatta tctcaggtat ccgaactgcc
     1141 ttagttttaa tcatcggtac ggctactttg gcagcattga tcggagcagg tggattaggg
     1201 acatttattt tgttaggtat tgaccgaaat acacctgttt tgacattgat tggtgcgatc
     1261 agttctgcct tgcttgcaat tatattcagc ggactgattc gctggttgca gcataagaaa
     1321 cctcgatatt ccatcattac tttagcagca gtggtgtgtt tatttggcgg cgtttcttta
     1381 tatcagtctg ccttgtttaa aaatgaaacc atcacgattg ctggaaaatt aggagcggaa
     1441 cctgaaatcc tgatccaaat gtataaagaa ttgattgaag acaacacaaa aacagaagtt
     1501 gaattaaaac cgaattttgg caagacgagt tttttgttca atgcactgga tcaaggtcaa
     1561 atcgatatct atcctgagtt cacaggtact gtcctagaaa gcttggtgaa ggtacctgaa
     1621 gcaatgaaaa gtgctgattt gacaaaagaa aaaacatatg acgaagcaga agaattattg
     1681 aataagcaat tcgatatgac attattaaaa ccaatggctt accaaaatac gtatgccttg
     1741 gcagtcaaga gatcttttgc gaacgaacat caacttaaaa agatttccga tttggcagct
     1801 atctctgatc agatcaaagc gggattcaca ctggaattta ttgatcgttc agatggatat
     1861 aaaggaatcc aagaagcata tggcttgaat ttttcaagtg tacaaagcat ggagccaagt
     1921 ttgcgttacc aagcaatcaa taatggagat gtcaatattg tggatgccta ttccacagat
     1981 agccagctca aggagtacga tctagtgact ctagaagatg atcgagggtt attcccagct
     2041 tatcaaggcg caccgctgat gaaaaaagat tttgctaaag aacatcctga aatcgttaaa
     2101 gcactgaata aattaagcgg aaaaataaca gaggatcaaa tgattgatat gaattaccaa
     2161 gtcaatgtcg aaaagaaaaa gccgacagaa gtagcacgaa tcttcctgaa aaatgaagga
     2221 ttagtaaagg aggaagatca gtgactaaat taatcgagtt caaacatgtg caaaaaaagt
     2281 atgatggaaa gtatgtcatc gatgacctaa atctggcaat caaaaaagga gaaatctttg
     2341 ttttggttgg tccttccggt agtggaaaaa caactacttt gaaaatgatc aatggattat
     2401 ctaaaccatc agcaggagac atctacttca aaggaaaatc gttaaatgaa tataatctgc
     2461 agaaaatgcg gtggaatatg ggatatgtgc tgcagcaaat cgctttgttc ccgaccatga
     2521 cagtcaagca aaatattgaa gtcatccctg aaatgttagg ttgggaaaag cagaaaagag
     2581 cagaccgtgt agatgaactt cttcaaaaag ttggcttatc tcctgatatt tatcgagatc
     2641 gtatgcctag agagttgtct ggaggagaac agcaacggat cgggatcatc cgagctattg
     2701 ctgcaagccc agatgtgatt ttgatggacg agccatttag cgcattagat ccgatttcaa
     2761 gaaactcatt acaagaattg gttttgtcct tacacgaaga attgggcacg accatcgttt
     2821 tcgttacgca caatatggaa gaagcaatca agctaggtga tcgtattgca tttatgaagg
     2881 atggagaaat tatccaatgt gacacaccag agcagttatt gatgaatcct aaaaacgact
     2941 acgttcggca tttctttgat gaaccgaaac agacgaaaga gtggcgagta gaagatttag
     3001 tcgtcaatgg atatttctta aatgaaatac cagaaaatgc tcgtcaacaa gtttgctttg
     3061 atacgcctat gaaagaagta tattcgcttc tttcggtgta tccaagcatt gtgatagtag
     3121 aaaagcaaag aagtattggg tcgctgacaa gtaaagaaat ctttgctttt ttaagtcgag
     3181 aggaggaagg aaatgaaaac atatgatgca atcatcatcg gttcaggtgt gagcggctta
     3241 agtgctgctt atggattgaa agaagcaggt aaaacagtac tagtggtcga agaagatcta
     3301 tggggcggga cttgtcctaa ccgtggatgt gatcctaaaa aagtcttgtt aagtgcagta
     3361 gaggcacgta atcgggtaaa acaattgagt ggaaaaggtt ttaacgagat cccaactgcc
     3421 aactgggaag aacttcaaaa attcaaacga acatttacgg atccagtccc agaaagccga
     3481 aaaaaacaat tagcagaagc tgaaatcgat catctttcag gaaccgctcg ttttttggat
     3541 gattcatcta ttgaagtcaa cgaagaagtc ttccatgcgg attacttagt tttggcaacc
     3601 gggcaaagac ctacgatctt accagtggag ggaaaggaat acttgaaaac tagtgcagac
     3661 tttttatcat tgcctgtact gcctaaagaa atcattttta tcggtggtgg ttatattgct
     3721 tttgagctag ctacgattgc caatgctgca gggagcaaag taaccattgt ccatcataat
     3781 caacggccat taaaagaatt tgaggcgtct ttggtagagg aagccgtcca tcaaatggaa
     3841 gcatctggca ttcagtttgc ttttggtgtt gaaacccaaa agatcatttc agaaggtact
     3901 cgctatcgtt tagtcggaaa agagacggaa ttagtagcag atatgatttt ttgtgcgact
     3961 gggagacagc cgaatacaga atctttagca cttgagcaag cgaatatcgt ctttgataaa
     4021 cacggaattg cagtcaatga ttatctgcaa acgagcaatc caaaaatttt tgcgtgtgga
     4081 gatattgttt caaggaaaac cccaaaattg acaccagtcg ccacttttga aggaaattat
     4141 gtagctaagc ggataacgga tgcaacaagc gaacctatca aatatccgat tatacctacg
     4201 atcgtctacg ccagtcctaa acttgctgaa gtaggagtta caaaaagtca cgcctcttcg
     4261 tcagatcaag tggtagagat ggatcttacc agttggttca cctatcatcg tgtcaatgaa
     4321 ccagttgcta aggctgaatt gacctttgac cagcaaaatt atttgattgg tgctgcagtg
     4381 attagcgagc aagcagacga attgatcgat gatttgacac tcgtcatcaa tcaaaaattg
     4441 actaaaaaag agttggatag ctatatcatg gggtatccga cattggcaag tgatctctcc
     4501 tatttattga aataatcagc atgataacta aagcgcttgt tgctttttcc ttcttgtgct
     4561 acactttaaa aaatgaatac ctgtttctgg ggatgtcgta gacgacatga gagtaagttg
     4621 tgaacttaga cccattgaac ctgtcagtta atactggtga agggagaaac atagcagacg
     4681 cttgaagcgg gttactatgt cgtgcccatt tcaagcgttt tttgttattc atattagaag
     4741 aaaggaggta acaaaatgaa gaaaaatctt tatccagcag tcagtatggc attcatttta
     4801 attatttggc agattgttgt catagtttgg aaaatacctg cctttatttt acccggacct
     4861 gctgctgtat tttctgcatt agcggcagat gcacaagtat tactttccca ttctgtcacg
     4921 acattaaatg aagcagtgct tggtttggta atagctagcg ttctttcttt tattactgcc
     4981 ttggcaatgg attattggaa gtttttagaa atgtcttttt atccgttact agtgatttca
     5041 caaatgttgc caatcatggt gttagggcca ttacttaccc tatggtttgg tttcggtatg
     5101 cttccaaaag tgatccttgt tgtcttgata agctattttc cgatcgtagt ttctttttcg
     5161 gatacattga ggaaagtatc aaaagagcaa atcatctttt tgaaaacgat gggggcagat
     5221 acagggaaga tttatcgaat atataaaata ccagcgggtt tatccggctt cttttccggt
     5281 ttaaaagtgg ctgctactta ttgtgtcagc ggagcagtcg ttggagaatg gctaagcgct
     5341 caagcaggct tagggtatta catgatacgc gtaaaaaaca gctaccagat cgataaagtt
     5401 tttgctgcga tcatttgtgt cattctattg agtttattgc tgaatggcgc ttgttcatta
     5461 ttgaaaaaag ggtattatcg tatgctttat attttttaag agaaggggat caaatgaaaa
     5521 aattatggct tttattaccg ctactgttgc ttttatccgc ttgtggcact gcaaaagaaa
     5581 caagtagcaa acaagaaata aaggatttga aaaaagtgac acttgttttg gattatgtac
     5641 cgaatactaa tcatactggc atctacctag caaaagaaaa aggctattat aaagaggctg
     5701 gtttaaatgt ccaaatcatc gaaccaggag ataattcgac aagtattggt ctagtaggtg
     5761 cggacaaagc acaatttggt gtaagttatc aagaagatgt cacttatgct cacgcagatg
     5821 gacagaatat accagtcaaa gcaattgcta cagtgatcaa acataatact tctggattcg
     5881 ccaccctatc agacagtaac atccattcac caaaagattt tgaaggaaaa acatatgcag
     5941 gttggcaaag tccaagtgaa gaagctgtct taaaagctgt gatggaaaaa gacggcgggg
     6001 acttttcaaa actgacaatg gtaggaagca atggagaagg gccagaaagc ttaggaaaat
     6061 cctcagatat ccaatggtac tttgaaggtt gggatatgat caaagcaaaa gaagcgggta
     6121 ttgaactaaa ctatatccca ttgaaagaat tagatgaacg gttagattac tatactccgg
     6181 taattattac gaatgatcaa ctgatcaaaa gtgatccaga attggtacag tcttttatgg
     6241 atgccacgaa aaaaggctat caggaagcta ttaaggatcc aaatgacagt gctaaactgt
     6301 tgcaaaaata tgcaaaagaa aatgatcgga cgtttttaga agaatcacaa gcattcttgt
     6361 caaaaaatta tacggatgat cctgagaatt ggggattgat ggaagaaaaa gtttggcgca
     6421 attatacagc ttttatgcag gaaaacggat tgatcaagca agatgtacct tcgcaagaac
     6481 tattcactaa tcagtttata aaataggagt gatagatatg tcaatccaaa tcagccactt
     6541 gaatgtagtc attgaaaacc tgaaaatatt agaagacatc catttcacta tccctaaaga
     6601 tacctttaca tcgattgtcg cgccaagcgg agccggtaag tcaacattgc tgaaaacttt
     6661 aacaggagtc cagcctatga cttctggaac gatcaaagta gacgatcaaa aagtaactgg
     6721 tctccataca gcatttagtt atatgccgca agaagatatg ttacttcctt ggctgagcgt
     6781 ctatcaaaac gttacgttat atcaaaaaat caatcatcta acaatcaatg aagaacaagt
     6841 aagggattat ctggatatct ttgggttaaa cggctatgaa cattttttgc cggaacaatt
     6901 atctggcggg atgaagcagc gaacggcctt gttacgaaca atcatgaatc cttcttctta
     6961 cttgcttctt gatgaaccat ttggagcgtt agatgctatg accagagggc agatgcaaga
     7021 ctggatgttg aagctgccta aaaaagcaaa gcggactact ttacttgtga cgcatgatat
     7081 cgaagaagca atttatttat cagacaggat tcttgtgctt tccgcccgtc cggcacatgt
     7141 gattgctgag attcaagtgc ctgaaaaacg acggtcacgt gaatggctac tgcagcaatc
     7201 agagttgaag caaacgattt atcagttatt ggcaggtgaa ttagatgtta agtgatgctc
     7261 attgtcattt agatgggagt cagagtttag cactccttca gcaggaacac acgattttga
     7321 cgatcatcaa ttgtgattcg ccagaagaat ggaaggaaaa cagacagctt gcagcaagta
     7381 agactcaagc cttaagttac ggtatccatc cttggaaagc agatagctat acgtttgaac
     7441 aagttgagcc ttttttgaaa aaagcaagga tcatcggtga aatagggtta gataatattt
     7501 ggacgaatgt accgatgact acacaaaaaa aagtatttga gcgacagctt gcttttgcag
     7561 ccataaatga gaaacctgtt gtactgcaca caaaaggatg cgaaaaagag atcttaaatt
     7621 atatccaagc ttaccctaat cactatttga tccattggta ttcttctttg gactatcaac
     7681 aagactatat tgatttaggc tgttatttca gcattggtct tgatttgaaa aagaatccag
     7741 cagtctggca actagctaaa gcagtcccaa tcgaccactt acttattgaa acggatggac
     7801 tacatgcagc agagtgggct ttagggggga ttttttcaga aaaagattat gtctcactgc
     7861 ttgaagaaca gttaaagaca atcgctgaga tcaaagagct atcagttgaa gaagttaaaa
     7921 aaaatactta tcaaaacttg gaacgtttca ttagattagg taaataaaaa agagatgtga
     7981 cacaactagt gtcgcacctc tttttggctt tctttgtata aaagtaaact gtttgattaa
     8041 taaatgaatc ttagtaccaa ccgtttgctt cccagaatgc tttcgccgct tcccaagaac
     8101 cataacgtga agtaacatat tgttctgcta ctctttcttg gtttgcagca gagtagtcac
     8161 catttaagta agaagaatct aattggtaac gtccgatgta acgtccgttc gttgctgtgt
     8221 aagaaccgct tgattctttt tgtgcgatcc attcttttgc agagcttgtg cttgctggag
     8281 tagcagcagt ttctgttaca gcaggagcag gttgtgtttc agcaacaggc gctgcttgtt
     8341 tttccgtttg aaccgtttct tgtacaacag gttgttcagc ttggactggt gcttcttgaa
     8401 cggctgcttg aggttcagca gctgcttgtg cagagccttc tgttgggatt gttaattgtt
     8461 gtcctgagta
//
LOCUS       DAYYHX010000119         8469 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000119 DAYYHX010000000
VERSION     DAYYHX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.120"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      54..133
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            131..1324
                     /gene="metK"
                     /locus_tag="ACHA6Y_001959"
     CDS             131..1324
                     /gene="metK"
                     /locus_tag="ACHA6Y_001959"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM8988044.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1454..2926
                     /locus_tag="ACHA6Y_001960"
     CDS             1454..2926
                     /locus_tag="ACHA6Y_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8988045.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3009..3797
                     /locus_tag="ACHA6Y_001961"
     CDS             3009..3797
                     /locus_tag="ACHA6Y_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8988046.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3864..4532)
                     /locus_tag="ACHA6Y_001962"
     CDS             complement(3864..4532)
                     /locus_tag="ACHA6Y_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM8988047.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4647..6359
                     /locus_tag="ACHA6Y_001963"
     CDS             4647..6359
                     /locus_tag="ACHA6Y_001963"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8988048.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6362..8134
                     /locus_tag="ACHA6Y_001964"
     CDS             6362..8134
                     /locus_tag="ACHA6Y_001964"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8988049.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(8216..>8469)
                     /locus_tag="ACHA6Y_001965"
     CDS             complement(8216..>8469)
                     /locus_tag="ACHA6Y_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8988050.1"
                     /translation="PISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         2588 a         1411 c         1855 g         2615 t
ORIGIN      
        1 aagcaaaaaa atgaaaaaat acgagcagaa aactgtttta tctgctatac ttggttcgtt
       61 gcaagttccc gaaaggatcc gcaggacgga gatgccttgt aaccgaattt aactaggggg
      121 aatcattata atggtagaaa gacacttatt tacatcagaa tctgtttctg aaggacatcc
      181 agataaaatt gctgaccaaa tcagtgatgc aatcttggat gcaattttaa aacaagatcc
      241 aacagcacga gtagcgtgtg aaacgtctgt aacaactggt cttgttttag tatttggaga
      301 aatatctacg acggcatatg tagatattca aaaagtcgta cgggaaacaa taaaagaaat
      361 tggttataca cgtgcaaaat tcggatttga tggagataca gcagctgtat tggttgcgat
      421 tgatgaacaa tctcctgata ttgctcaagg agttgatgaa gcacttgaga tccgggatga
      481 agataaaaaa gacgtattag atgagattgg tgctggtgac caaggtttaa tgtttgggtt
      541 tgctgtagat gaaacaccag aattgatgcc tttaccaatc gctttgagtc atcgtctggt
      601 acgacgcttg gcagatttgc gtaaatcaaa tgaattaacg tatttacgtc cagatgcaaa
      661 atctcaagta acggttgaat atgatgatca aggacaaccg gaacgcgtag atacaatcgt
      721 tatttcaaca cagcatgatg atgcagtaga taatgaaaca attcgtcatg atgtcatcga
      781 aaaagtagtg aaggaagtta ttccagctga attattagat gatcaaacga aatattatat
      841 caatccgact ggccgatttg tcattggtgg tcctcaaggg gatgccggat taacaggaag
      901 aaaaatcatt gttgatacgt atggcggtta tgctcgtcat ggtggcggtg ctttttctgg
      961 taaagatgcg acaaaagttg accgttctgc cagctatgct gcacgttata ttgctaaaaa
     1021 cattgttgca gcagggcttg ctcgcaaagc agaagtacag ctagcttatg cgattggtgt
     1081 tgctcaacct gtttcgatct caatcaatac tttcggtact ggaacggttc cggaagaaga
     1141 actgattgct gcagtgagag aaaacttcga tcttcggcca gcaggaatca tcgagatgct
     1201 tgatttgcgt cgtccgattt acaaacaaac agctgcttac ggtcattttg gtcgtacaga
     1261 tgtagatttg ccttgggaac atacggacaa agtagatgcg ttgaaagcaa gtttagcaaa
     1321 ataagttata ttcagctgga acgatcgcca agtggtggtt gttccagctt tttttatggc
     1381 agttaacctc ttgaaaaaga tagtaattat ttatctattt ttagatcctg tcataatgaa
     1441 agggaggaat aagatgaaaa aagaaacaaa tgttactttg gtcacagtga gtattttcat
     1501 tgcaacgttc atgacggcta ttgaaggtac aatcgtaaca accgctatgc caacgattgt
     1561 cggttcatta catggaatgg aaatcatgaa ctgggtattt tcgatttatt tgctgacaaa
     1621 cgctatgctt actccgattt acggaaaact tgcggataaa gtgggacgga aaccaatctt
     1681 tatttttggg acattattgt ttattatcgg ttcgttattt tgcggtttat ctcaaagcat
     1741 gattgggttg attgctgctc gtgcgattca agggatgggt gctggggcaa tgatgccagt
     1801 agctttgacg atcatcggcg atctttattc tattgaaaag cgagcaaaag ttttaggttt
     1861 gaatagttca gcctggggga ttgccagtgt ttttggaccg cttgcgggag gattcatcgt
     1921 tgaaacgatc agctggcact ggatcttttt tatcaatgtg ccgatcggaa ttctattaat
     1981 tattttgatt tggctatatt taaatgaacc aaaaatcata catgagtcta aaccaatgga
     2041 tatttttggt agttttagtt tgatggccat gcttgtctca ttgctgttag ggttccaatt
     2101 attaggagac gaaggaatga gtccgcttgt ttctgctttg ctgctaggta gtatcgtaat
     2161 gttaggtatt tttgtttttg ccgaaaaaag agctaaagat cctgtcatca tgatgcattt
     2221 attcaaaaat ccactatttg ttgtggtcaa cttggctgca gcattggcta gtggtttttt
     2281 gatgggaatc gatgtttata tccctatgtg gatgcaaggt gtattagggc tgaatgcagc
     2341 aattggtggg ctggtattag caccattatc actagtctgg atggtcggtt ctttccttgc
     2401 aagcaagtgg atgactcaaa tgaaaactca ttgggtcttg agaattggac tactaatcgc
     2461 agctattggg ggcgcttggt tgtatcttat gcctttcaga agtaactatt tatggttttt
     2521 tgctatatca acggtcatcg gtataggctt aggtgtgacc atgactacct cgactgtcag
     2581 tgcacaaaat agtgtatctc gtgaccagct tggtgtagca acttctttta atacgttagt
     2641 tcgaacaatt ggtcaaacag tcatggtttc tatttttggt ttattgataa atggggtaac
     2701 taaacgagaa cttgcagcag caaatttaac aaatgatgca gatatcatga ataaactggt
     2761 gaatccacaa acggctaaat atattgatgc ggatattttg ttaccattaa gaaaaattct
     2821 ttatgatggg ttacattccg tttatctagt tggcttgtta ctcgtgatca ttgctttggt
     2881 cttaacgctt ttcgttcatg aaaaaagtgg taaaataaaa cagtagtttc ggttaagata
     2941 tgtacgtagc cgaatcattc aaatttaaag ttattcaaaa ataacttctt actaaggaag
     3001 gcgaaaatat ggcatttatt caagcaaata tttattcgaa tgtgttagaa atggaagtca
     3061 atctaaatgt gatactaccg caaacaacaa aaaaggtgat tggcaccagt tcagagcaag
     3121 agacaacgga tatacctgtt ttatatttac ttcatggaat gggtggcaat catagtgtat
     3181 gggaaagacg tacgtcgatt gaacgttatg tagcaaacta cggattagct gtcatcatgc
     3241 catctactga tcttggctgg tacacagata cagtttatga catgaagtat tggacattca
     3301 ttgcagaaga gctacctgag atttgtcatg aattattccc gcaattaaca agaaaaagag
     3361 aaaaaacata tgccgctgga ttatcgatgg gcggatatgg cgcgttaaaa ctaggccttg
     3421 caaaatcaga aaatttcgca gcagtaatct ctctttcagg cgctgtttct gtaggaaatc
     3481 gaatggatga tcttttgatg gttcgtaaag catctttttg ggaagggatc tttggaccat
     3541 tggatcaaat cgaaggttca aaaaatgatc cgcaatactt attgaaagaa ctagtaaaaa
     3601 gcggaaaacc agttccaaga ttttatatgg cttgtgggga atctgatttt ctttatcagg
     3661 caaatcagga aatggtatct gctatgcagg aacaaggctt ggcggtcacc tttgaacaag
     3721 gacctggcga tcataactgg atcttctggg accaatggat tcaacgtgca ttggcgtggc
     3781 taatggaaga aaaataaaaa aagagtctta aaaagatagc tcctatagag atcacaaaca
     3841 tagtgatctc tataggagct tttttactgc tcgttttctc ttcttttatt ttgttggcat
     3901 aagccttcaa tataggtttg tgcactcgtt agaaagagtg ctttttcatt caattctgca
     3961 tgggactgca tactttcttt aaatccctct aattgatttt caactacata attacgcata
     4021 aatagaactg tttctttcaa aaattcattt ccgtccataa ttttttgtcg aagctgctgt
     4081 tcatgctgct gatccattaa ctgtccaatc aataaattgt ggagagaggc aatggcctga
     4141 tgactttggg tgactgaaaa tccttcacga acaagtaatt ggatcatttg ctccaaatgt
     4201 tctaatcgac tcggaaaagc gggcacagta ttgatttcta attctgcccc acaagggaaa
     4261 cgtgtataaa ggtcatagta attttccata tactgcagca actgttcatt ccaaggcaaa
     4321 gttggatctg gaagcgctaa ttcttcttcc attgtttcag ccattttttg caagagctgt
     4381 tgtttgtttt tgaaatacca atataaagca ggtgcttgaa catgaattct gtttgcgatg
     4441 tttcgcatgg aaagctgcga aagagcttgt gtttcagcca ggatatcaaa ggcggcctga
     4501 atgatgattg cttgagaaag ctttatttcc attttatcaa cctcgtgtat tgcattatct
     4561 ttttaaacat ggtaaattgt tagtcggtct aattttacag tgttaaatta gtcctgtcaa
     4621 tttaaacaaa ggggtgtaat caacgaatga aattaatctt acgacatgcg aaaaaatata
     4681 aagtatctgt ttttatttcc ttgctgtccg tagcagtcat ggttgcagca gcattatggc
     4741 aacctaagtt actacagcaa gtattggaag ctattattac agaagataat gatgaaatga
     4801 aaacaattgg tatttacctg atcagtctgg ctttgctagg attagccgca ggtgtaacga
     4861 acacgatttt ctctgcaaaa gtagctcaag gcgtaagtgc agatatccga gaagaagcat
     4921 ttcgaaagat ccagacattt tcatttggaa atatcgaaca gttctctgct ggaaatcttg
     4981 ttgttcgtct aacaaatgat atcacgcaaa tccaaaattt agtgatgatt tctttgcaat
     5041 cactttttcg aattccattc ttatttattg gtgccttcat tttagctatg atcacgatgc
     5101 cgcaattatg gtggatcatc gtagcgctta tcgttacggt atttctgatt acggcacttt
     5161 catttacaag gatgggcaag cacttcatga tcattcaaaa actgattgat cgtgtgaata
     5221 gcattgcaaa agaaaactta ctagggattc gtgtagtgaa atcttttgta caagaagaaa
     5281 accaattgaa gtcattcagc aaagtaagtg aggacttaac aaaacacaac atcatagtcg
     5341 gtacattatt ttctgtgatg atcccgtcct tcatgctggc tgccaatttg gctgtggttg
     5401 gggcgatctt ttttgtgagc gatcttgtta aagatgatcc tacgttaatt ggtggaattg
     5461 cttcattcat gaactacttg atgcagatta tgatggcaat cattattggc ggaatgatga
     5521 tgatgatgac ttcaagagca gccgtatcgc tgaagcggat tgcggaaatt ttagatgctg
     5581 aaccagatct tacttattta gacgttccag aacaagagtt gacgggttct gtcacttttg
     5641 accatgtttc ttttcgttat cctggtgagg atacagatac gcttaaagat atcagctttt
     5701 ctatcaaacc tggagaaatg gttggtatcg tcggggcgac tggtgccggg aagtcaacac
     5761 tagcacaatt gatccctcgt ctatttgatc cgacagaagg caaagttgaa gtaggtggtg
     5821 tagatcttcg tcaagtaaat gaaaagagtt taagacaaac ggtttccttt gttttgcaaa
     5881 aagcaatttt attctcagga acgattgctc aaaacttgcg ccaagggaaa aagaatgcaa
     5941 cagaaaaaga gatggaacat gcttcttcta ttgctcaagc aaaagaattc atcgaaaagt
     6001 tatctgatcg ttacgaagca ccaatagaag aacgtagcag taacttttct ggtggtcaaa
     6061 agcaacgttt atccattact cgtggcgtta tcggtaatcc aaagatcttg atcctagatg
     6121 acagtacgag tgcactggat gctcgctctg aaaagctggt ccgagaagca ctcgatcgag
     6181 atttgaaggg aacaacgacg attgtcatcg cacaaaaaat tgcttctgtt gtcaaagcag
     6241 accgcatctt agtattggat gaaggcagat tagtcggcga aggaacccat gaggaattgg
     6301 tagctggaaa ccgtgtctat caggaaatct ttgaaactca aaaaggaacg gaggagtaag
     6361 tatgactgat ctaataaaag caagtaaatt tttctatcac tatttgaagc ggtacaaact
     6421 ctcctttttc tttatcttta tcacagtgat cattgctacc tatctacaag taaaggcacc
     6481 tcaatacatc ggggaagctt ttcaagaatt agctaattac gtaggaggct tgatgcaagg
     6541 cgtagacgat aaaagcaaat ttgtttctgt aatatggaaa ctcttgctgt tttatgtact
     6601 agctagtgca gccaacttta tctatagtat tttgttcaca caagtagtag ggaagtcaac
     6661 gaaccgaatg cgtatcggtc tattcaacaa attggaaaaa ttgacgatcc gtttctttga
     6721 ttctcatcaa gacggtgaaa ttttaagtcg ttttaccagt gacttagata atattcaaaa
     6781 tagtttgaac caagctttgc ttcaagtcat cacgaatgct gttttacttg tcgggatctt
     6841 gatcatgatg ttccgccaaa atgtagagtt agcttgggct acaattgctt ccactccagt
     6901 agccgttttg atagctgtct tgattatccg caaagcacgg aaatatgtgg atcttcagca
     6961 agatgaagtg gggaaactaa atggttatat ggatgaaaaa atcagcggac aacgtgtcat
     7021 catcacaaat ggcttacaag aagaaacgat tgaaggattc ttagagcata atcaaagtgt
     7081 tcgcgaagca accttcaaag gacaagctta ttctggtcta ctatttccga tgatgcaagg
     7141 aatgtcactg gtcaatacag cgatagttat ctttttcggc ggctggttag cattaaatgg
     7201 tgatttggaa cgttcggtgg cattaggctt agttgtgaca ttcgtccagt attcacaaca
     7261 atattaccaa ccactgatgc agatttcatc tggatacagc atgatccaat tagcgattac
     7321 aggggcaaga cgattgaatg aaatatttga tgaaaaagat gaagtcaaac cagaaaacgg
     7381 taaagcattt gatggtgttg aaaaaggtct tgcgttgcag cacgtagatt ttggttattc
     7441 tccagaaaaa ctagtcttga aagatgtctc gatcaatgtg gacaaaggag aaatggttgc
     7501 tcttgttggt ccaacaggat cggggaaaac gacgatcatg aatctgctaa accgttttta
     7561 cgatgtcaat gatggttcag tagtcatcga tggaatagat atccgagatt atgatcttga
     7621 tgcattacgt agacatgtag gaatcgtttt gcaagaatct gtattgttct ccggaacgat
     7681 caaagacaat atcacattcg gcaaaccgga cgcttcagat gaagaagtca tcaatgcagc
     7741 aaaacaagcg aatattcatg acttcatcat gacgttagaa aaaggttatg atacagagat
     7801 atccgaagaa aataacttat tcagtacagg tcaaaagcag ttgatcagta ttgctcgaac
     7861 gatcatcaca aatccatctt tattgatttt ggatgaagca acaagtaatg tagacacggt
     7921 aacagaagca aggatccaaa aagcaatgga tgaagcaatc aaaggaagaa ccagctttgt
     7981 gattgctcac cggctgaaaa caatcttgaa tgcagataga atcgttgtct tgcgtgatgg
     8041 ggaagtcatt gaagaaggca gtcatcatga gttattagca gaaaatggat tttatgcgga
     8101 actttatcat aatcaatttg tatttgaaag ttaaattaaa agaacctcgt tataacgggg
     8161 ttctttttat atattttatg ttgtttgttt ttataagggg cagaaaaggg gcaatttaaa
     8221 tttctatttt tcctagtttt tctactagtt gattttgcat atttttagtt gtatgagtat
     8281 aaatctttaa tgttgttttt gaatcttcat gtcctacacg atccataatg gcttttaatg
     8341 ggattcctaa ttctgtaagt agagaaatgt gggaatgcct taacatgtga gaagaaactt
     8401 ttttatctag gtttaattct ttggatactg ctcttaaaga ttgattatat gcttgaatag
     8461 atatcgggt
//
LOCUS       DAYYHX010000120         8372 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000120 DAYYHX010000000
VERSION     DAYYHX010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.121"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..1373)
                     /gene="celB"
                     /locus_tag="ACHA6Y_001966"
     CDS             complement(21..1373)
                     /gene="celB"
                     /locus_tag="ACHA6Y_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8988051.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1373..1891)
                     /locus_tag="ACHA6Y_001967"
     CDS             complement(1373..1891)
                     /locus_tag="ACHA6Y_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988052.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1907..2233)
                     /locus_tag="ACHA6Y_001968"
     CDS             complement(1907..2233)
                     /locus_tag="ACHA6Y_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM8988053.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2246..4225)
                     /locus_tag="ACHA6Y_001969"
     CDS             complement(2246..4225)
                     /locus_tag="ACHA6Y_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8988054.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4246..4560)
                     /locus_tag="ACHA6Y_001970"
     CDS             complement(4246..4560)
                     /locus_tag="ACHA6Y_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM8988055.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4728..5021
                     /locus_tag="ACHA6Y_001971"
     CDS             4728..5021
                     /locus_tag="ACHA6Y_001971"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM8988056.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5099..6211)
                     /locus_tag="ACHA6Y_001972"
     CDS             complement(5099..6211)
                     /locus_tag="ACHA6Y_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8988057.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6364..7212
                     /locus_tag="ACHA6Y_001973"
                     /pseudo
     CDS             6364..7212
                     /locus_tag="ACHA6Y_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7565..>8372)
                     /locus_tag="ACHA6Y_001974"
     CDS             complement(7565..>8372)
                     /locus_tag="ACHA6Y_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8988058.1"
                     /translation="GYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSG
                     EVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPI
                     EFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLT
                     TGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVAD
                     SSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2605 a         1722 c         1372 g         2673 t
ORIGIN      
        1 agttcctttt ttcttagaaa tcattttaac ttttcactag ctaattctgc tgccttttct
       61 ctttcttcat ttaataattt attatcatag aatttgataa atgggaacca gataatgaat
      121 gcgatgattg cacaaatgac tgctaacaca gcagctttcc aatcaccacc actaccaaca
      181 aatgctccga tcccgactgg tgaaggccac ggcatctgtg caagcatcgg tttgacgatt
      241 tccagccgaa tggcaaaata agcgagtgta gcagaagcca tcggagctaa gaaaaacgga
      301 accgctaaat agggattgta gacgattggc atcccgaaaa tgattggttc attgatattg
      361 aataatcctg gtacaagtga ggcttttcct aacactttca gctgatcaga tttcgcgagg
      421 aatgcaataa agatgatcaa tcctaaagtc gcacctgatc caccaacggt tacataagaa
      481 ttgttgaatt ctcctgccaa cggataattt gcgccttgtg cgttagctgc catattagct
      541 aatacgatcg gggtcaagaa agaagtgatg atattcgctc catggatccc tacgatccac
      601 aatgcatgga tcaagaaata aatcaccatg acaccaagcc atgtattcgt caactgcgta
      661 acaaacccaa atgggatagc aatcattttg aagatatccg ttcctagtgc aactaataat
      721 ccattgatga tcatcaccac aaacgcaatg acaaatgttg ggatcagagc agtgaaagaa
      781 cgagaaactc ctggcggaac aacatctggc atttttatga cccaatttct cttgacacac
      841 atacaataca attcgaccgc aataatcgac ataataatag ctgtaaagat tcctgaagtt
      901 cctaaacgtt cgacaaaaga gcccatccta ataccattta ctacagtttc tgtctcattg
      961 atagaagcaa tcaagctgat tttaccatca gtcatgatca attcagggat acacataaag
     1021 aatgcaaaaa cagatagtaa tgcccccgtg agcggattaa catttaattt ttgttcatct
     1081 cgttcaattg acgttaattc ataagcaaac acaatggcaa agtaaataga taaaatcccc
     1141 atcgtcattg tattgacgat catgtacaag tcactaactt tgaaaaaagt tgcattaaaa
     1201 aatccttcta aaaatgggaa cggtaatggc aaaatattta aaaccaaaaa catcgagcct
     1261 acaatcgtga aaggaataga agccatcccc gctgccatga cagcccgaac gattttaagt
     1321 tgcgcaattt tccccatagg tcccatcagg tatttctgca aaaaatcaaa cattagttat
     1381 tctcctttcc taaatataac gctttttcat attgtttaat acggatatac tgttggtctt
     1441 ggttgagtga aattttcttt aaacgatact gtataaatgc actggcaaaa ataccgctta
     1501 caacaagaat gaaaacaaat aactgtcctc cattgttatt tgccataaaa ggatacaaga
     1561 atgtaaatcc tgaggtaaac aacactggaa tcactaataa attaaagaca ctctggaaca
     1621 aaaaatacca ctttgtccat ggaacgttcg tttggtgttt tcgataaagc tttacttgtt
     1681 ctcctactgc aggcaaaata gctaaaatca gaatcatcgg taaactactt cctaaacttt
     1741 tagctgatag aagcagaatc atccattgca tgttcatgaa aaagaaaaag gcagtcaggt
     1801 aacgtacgat tagataacga ttgaaataca tgtttttcaa actaagttgc tttttttcta
     1861 attcgatttt cctttttact gtatcctgca caatgatcct tcctttctat ttattttttt
     1921 gatacaaata attcatttcc tgtgcaactt ccaataatgt catcgtcgtc atcagatgat
     1981 cttgtgcatg gaccatgata atttccattt ctatttttgt cccgctggca tattctttca
     2041 gaagttgagt ttgtgactga tgcgcatgaa gaatctcttc atttgcttgt cgcaacttct
     2101 cttctacttc ttcaaacatc tctttacgca tcgagcgaaa tgcttcatgt atcagtgtcc
     2161 gtgcatttcc gctatggaaa atgatatcaa aagaagtcac ttgtagtgtt tcactgttta
     2221 ttttttcagc catctgtttt cctccttatt cgatcagttt gataaattta ctgctgaaat
     2281 tctcaaaaat aggttcagct aagatttcct gctgtacttt tagctggtca actaacctaa
     2341 cgattgcttt agtcacgacc gtaattcctt cattttcgac ataagaaggc gaaactaaaa
     2401 aaacaaaatg aatttcttga tattgatccc actgcatccc agaaggaatc aatgcaacgg
     2461 ctacctttgt ttcgattcca actggaacag cgggatgtgg gactgccact gtatcactga
     2521 atatgatttg tcccatattt tcacgttgct ggatctgctt tcgcatttct cttatatatc
     2581 cttcttcttc atttatgctc agcttttgca gcaaatcatg aagaatttct tcctttttcg
     2641 gttcgtacgt ataagtcttg aaaaattttt cagacaactg ttcggtgtaa atctgctttt
     2701 tttgctgtag agaaaatgct ggtatcggtc tatctgagga atgatagggt atagattctc
     2761 caactacctt tcgtatttta cgaacatcat cttcattcaa aaaaatactg acatgtaaaa
     2821 ctggaatttt gaaaaacatt gtcgaaagat caatagaaga aataatcagg tcgattcctt
     2881 ttaaagttgt ttcattgatc tcataatagc cttttacact taccactgta atatttttac
     2941 cgaattcact gactacgcga ttctttaata gttgggcact gccgtaaccc gttgcacaga
     3001 taatcaatgc atgtagttta tgcgcagctc tttctttttc caatgccgcc atgagatgca
     3061 aagccaaata ggcccattca tcttcattga tggtatagtt tttcaaagcc ggcatgttgc
     3121 tgaaatgcct ttttactgcg tcaaagactt tgggattttc tttctttatc tcctcagtca
     3181 atgggttctc aatgcgaata cctcggctca atcgaatcat catcggtgac aaatgatcga
     3241 gtagtccatt gaaaagctgg agatcctcta ctaatgaaaa atgaaattcc tgagacaatc
     3301 ttttcagaac tttcgctaat tcctttgata gttcctgatc attttgattt tctttgtgat
     3361 tcgacttagc catgagatgt aatgtcaaat aagcaatttc ttcttcggga aacgatacac
     3421 ctgcgattac ctcaatacgt tgaacgatcc gctgcgccac ttgatactct actcgcttag
     3481 aaacttcttg ttcgatgcct aattccttga tttcaaaccc ttctcggagc cgtttgatcc
     3541 ccaaagccaa gtgtaaagtc agattctgta tgataaaatc cgaaactttt aacttagctt
     3601 ctcttgtttc atcgagaatg ataatgacta gctcttcaaa actgatgtct gggaaaaaac
     3661 ggctttgccc taaatattct ttcaatgaat ttccataatg gtttccaaaa aaagtatcca
     3721 tgataaaatg ccgtcgattt ctttcttccc cttcgatcca tgtcccttga ccatgcttag
     3781 aaacgatttt tagtgaatat ggaatcaatc gctccttgat ttcttgcatg tcctttgtca
     3841 aactggaacg actgatataa agctcttctg ccaaatcatc cataaaaaga atggcatctt
     3901 ccaaaagcaa ttggttcaat atatactttt gtcgatcttc ggactcataa aatccttgcg
     3961 gttctctagc atatccaggt actatttctt gccgactcaa aaacaagtca aatgtcagtc
     4021 tttgtaaaat atgcagttgg tagccataac ctggtttagc aatgatctta cctccgtttt
     4081 tttctactaa ctctttgatc cgctgcaagt aattacggat tgtccgatca gataataaca
     4141 attcagaagc taaacgttga ctggtgacat actccttctt gtgatcaatc aaatatctca
     4201 acagcaattt ttccttctgt ttcattatca tccactccta ttccatcata tgttctcttc
     4261 gatcacctct gctaacttct ggatgcctgt agggattggg acatacgctt ggaaagggat
     4321 actaacaaca ggctttccaa cagattctcc taattttttg aatttatcca aaaacatcgt
     4381 cgtttgcggg ctgatcagaa ataaatcaaa atcactattc ttaatcatat tatcgccttc
     4441 tgtagctgaa actgcatcta cgagtacctc ttctcttttt tgcttaaaga aatccgttgt
     4501 ctttgaggcc atcatcgagg atgacattcc tgccgcacaa ataatcaatg cttttttcat
     4561 aaatcaatca ctccttttat ttttataaac caagcatatc ttgtaagcgt tttctattca
     4621 aaatatatat ttccgtagca ttgcggaaaa aatgtgtatc atatcttgaa taataatttc
     4681 tggcaccgtg atatactagt gatagaattt tttcaggagg ggatttagtg agtatcacag
     4741 tgaatatttt gtatacaagt acaaatggac aagccagaaa atttgcagaa gaaatggtcg
     4801 aaagaaacat tgtcaaagcg attcgtgcag aagaaggaaa cgagaaatat gactattact
     4861 ttccactaga agatccagaa tctcttttac tgatcgaccg ttggaaagat gaagaagcaa
     4921 tcgaaaagca tcacaaatcc gaaatgatgg ctcaaataaa cgaactccgt aaaaagtata
     4981 aattaaaaat gaaagtcgaa cgtttcactg atttaccgta aaaagataca catggaatga
     5041 tagcaattta tcatccatgt gtatcttcac tttttcctta ttttttattc gatctcatct
     5101 aatgattcaa accgtgtttt tctcttattt ttcggataag tatttcagtc accccaatat
     5161 acatgattcc taaacatacc ccaaggatat tctcaaaaat gcggatcgtt actgctcctg
     5221 gaaccccgaa taaactggca gtgattgcca aagcaccaaa agaattaaaa actgttttat
     5281 atctataggt agaacaaaca cctaaagcta gaccacctaa tataccgacc cagttaaaag
     5341 gaataaattg gctgatacca ataaataaaa cacatccaat caatgagcca atgattcggt
     5401 ctactgctcg ttctttgaaa ccaatcgaca tcaatccata gctagataca atagaagaaa
     5461 atgcaaaccc tgcccacatc aatcgctgaa aagggacata ctctcctgca acgatcaaaa
     5521 gactaatgcc aaatgcatag atgagcatcc aaattttttc ctgcgaatac atgcctttca
     5581 aaaaaatctc tttaaacaag cttttgtctt tgttcttttc tcgatgtttt ctgtaaagga
     5641 gaactgaaaa ccaacaaaaa aacaaaaata ataagctact tgtttgtgta aagaaattct
     5701 tgttcaacga atctttaggt aacgaataaa caataaagag atatgaaaag ccgtacaaag
     5761 aagcattccc cattttggga tcactagcgg ttgccattaa tatgctgctt agtaaaataa
     5821 aatgaatcaa catgcccata actgaccatt tgatcagctg gacatacggt gctactaata
     5881 gtgagaacat gacgattgct aaacctatga tcgaatggga aatcttataa ccaaagtcaa
     5941 caaaacgaat actcatcaac atacagaata aaaccacagc gtatggttta tgcttcgatt
     6001 caaataatgt tgtaattata atgataaaac aaatggcaaa caatacgatg aataatgatc
     6061 gtaaaaacaa tgaacaaata tacatgcact tttctttttt agaagctgct tctcgtattt
     6121 tttgttttaa aataaacgga tctagttgta gagactgata aaaatccacc tgttgtcgtc
     6181 ctttcttttt acttttattt tttacagtca tctatgacag gttgtaaata ctatggaaca
     6241 tcatatggcg ttgtcatttt cttgtcaact atttttccta ttcatgtttt tataaaaaaa
     6301 catatcgttt tcttgtatga ttaaatcacc acaaaataaa caactgagag aacgatatgc
     6361 tttatgaatg gttctatcaa aaaaattctg agatcaatag acaaaaattt gatgattaca
     6421 gaggtctctt acgaaacctt acagaagaaa aaaacattga tcgttgctgc agttctcttc
     6481 cctgctccat gtgtctgtag aaattgtggt tctgctgtgg tagatggaaa taggaaatta
     6541 atggtggtga aaaacaagaa gaaggaaacc attgtccact ttgaataata taatcatatg
     6601 ccaatggtta tacgcctaaa aaagcagcgc tatacctgta aaaactgtcg aagccattgg
     6661 aacgctcaaa gttattttat ccgtccaagg cactcgattt caaatcatgt cagacataaa
     6721 atcacttctt tactgactga aaaagtgtcg ttatttttta tttcgaaaag ttgttaggta
     6781 tctttgacaa ccgttattcg cacattgaaa gagttgagaa gctatttacc aaagtcttcc
     6841 aaaagaattc tgtcttgagt gttgagggtt gtgaatttcg ttctcacgtt tccgttgagg
     6901 ataaactgag ttttatttcc gcggatggcg agacagaaaa attaatcgag aaaatttacc
     6961 tacacaaaaa attcctcgat taacaaacta tttccttagc tgcactaatc ctaaagaaat
     7021 agaattctta gttacagaca tgaacgccac ctactttcag ctcaccaaac atgttctacc
     7081 aaatgcgaaa ttggtgattg atcgattact tggtttttcc acgcctttca aggaggcgta
     7141 acctttattc acgagttggt tgaagctttc cgagacaaaa gacccgactt gtttttctcc
     7201 ttattgacaa agagccatta aaactagaga aaaaattaca actgatcgct gattatttgt
     7261 agaaagaaaa tcaaagtatt acccaataaa cttgagtcat taggcaaaaa acactgcgaa
     7321 actcaagaat gattttcgca gtgtttttgt atatttttta ttaactagta ttactacaaa
     7381 ggtttatttt tcacttctca acgcagcaca aaattgtctt tacttattta ctggttgggt
     7441 tcaaccttct ctactccgat actaatattt gtttgtatac cccttctaaa atcggtagtt
     7501 tccattcaat tgatgggtac tagttgattt taaaaactat gcttattcaa ttttttattt
     7561 tagctcatat atttcgtttc tttctaaatt ttatactgat gaaaacaagt agcacaccca
     7621 atattgacaa tataaactgg ctaatgatgg tttctccagt ttttggaaga tgcttatttc
     7681 cactgcttgt tggattgttt cctcttatat ctgagctatc agcaactttt ctattctctt
     7741 ttgttaaaaa gatcgccact tcttgttctt tttcgatctt gaaagttact ggtgtcgcat
     7801 ccaattcata ccccgttggc gcttgagttt ctaccagctg atatgctcct ggtgctaagc
     7861 catctatcgc cagttttcca tctgctcctg tggttagacc tgactgcaga acggttccat
     7921 tggcatcttg taactcaaaa acagctcctt gcaggacttc tccgctttgg tcatcgatct
     7981 ttgtcaacac gacaccgcct ggtgtaagtc gattttcttt cgtcaattct accaccgctg
     8041 tctgacttct ttcgatctca aactcgattg gtgtcgcatc caattcatac cccgttggcg
     8101 cttgggtttc taccagctga tacactcctg gtgctaagcc gtcaatcgcc agttttccat
     8161 ctgctcctgt ggttagacct gactgcagaa cggttccatt ggcatcttgt aactcaaaaa
     8221 cagctccttg caggacttcc ccgctttggt catcgatctt tgtcaacacg acaccgcctg
     8281 gtgtaagtcg gtttgctttc gtcaattcta ccaccgctgt ctgacttctt tcgatctcaa
     8341 actcgatcgg tgtcgcatct aattcatacc cc
//
LOCUS       DAYYHX010000121         8328 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000121 DAYYHX010000000
VERSION     DAYYHX010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.122"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..2252
                     /locus_tag="ACHA6Y_001975"
     CDS             87..2252
                     /locus_tag="ACHA6Y_001975"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM8988059.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            2556..3389
                     /locus_tag="ACHA6Y_001976"
     CDS             2556..3389
                     /locus_tag="ACHA6Y_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM8988060.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(3491..5191)
                     /locus_tag="ACHA6Y_001977"
     CDS             complement(3491..5191)
                     /locus_tag="ACHA6Y_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988061.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            5698..6858
                     /locus_tag="ACHA6Y_001978"
     CDS             5698..6858
                     /locus_tag="ACHA6Y_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8988062.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6874..7317
                     /locus_tag="ACHA6Y_001979"
     CDS             6874..7317
                     /locus_tag="ACHA6Y_001979"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8988063.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(7800..>8328)
                     /locus_tag="ACHA6Y_001980"
     CDS             complement(7800..>8328)
                     /locus_tag="ACHA6Y_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM8988064.1"
                     /translation="KEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMVK
                     GRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPEI
                     TVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPDD
                     IRSGKYFDENYKRVK"
BASE COUNT         2749 a         1426 c         1730 g         2423 t
ORIGIN      
        1 aaaataaaaa ataaagcatt tacaaaaaag aaaacgcacg ctataatacg tttaacgaaa
       61 ccgattgcgt aaaggagcta aacggaatga aaaagatatt tagttttgag ttttggcaaa
      121 aatttggaaa ggctttaatg gttgtggtag cagttatgcc ggcagctggg ttgatgatca
      181 gtatagggaa atcaattccc ttgatcaatc ctgattggac gccgcttgtc acaacgggcg
      241 gagtaattga aaatattggt tgggcaatta ttggtaatct tcatatttta tttgctttag
      301 caataggagg aagttgggca aaagaaagag caggcggagc atttgcagct gggatctcat
      361 ttattttaat caatcggatc actggggcta tctttggtgt cacaagtgac atgcttgcga
      421 atgaagatgc atttacccac actctgttcg gtacgaagat catggttaaa ggatttttta
      481 caagtgtatt ggaagcacca gctttgaata tgggagtatt cgtagggatc atcgctggat
      541 ttgtcggggc aatggcttat aataagtact ataattatag aaaattgccg gatgctttgt
      601 catttttcaa tggaaagcgc tttgttccgt ttgttgttat cttatggtca acaatcgtgg
      661 cattgattct tgctgtggtg tggccaaacg tacaggctgg aatcaataat tttggtttat
      721 ggattgcaga atcacaagaa agcgcaccga ttctagcacc gtttttattt ggaacattag
      781 aacgtttact attgccattt ggattgcatc atatgctgac gattcctatc aactatacac
      841 agcttggcgg atcgtatgag attttatcag gcgcacaggc ggggacacaa gtatttgggc
      901 aagatccgtt atggttggct tgggccactg atttagtgaa tcttaaaggt gcaggggata
      961 tgtcgcaata tgagtttgtg ttgcataact ggacgccggc tcgtttcaaa gtaggacaaa
     1021 tgattggttc ttcaggtatc ttgatgggaa tggcttttgc gatgtatcgt aatgtcgatc
     1081 ctgataagaa agcaagatat aaatcaatgt atttttcagc agcgttggct gtctttctaa
     1141 caggtgtaac agaaccattg gagttcatgt ttatgtttgc tgctgtgcca ttatatgtca
     1201 tttattcagt gatccaaggg gctgcatttg cgatggcaga tatcttgcca ttgcgggtcc
     1261 attcatttgg gaatatcgaa ttgctgactc ggacaccgtt agctatcaaa gcgggtctag
     1321 gtggagatct tatcaacttc gtgttgtgtg tgatcgcttt tggtgttgtt acgtatttct
     1381 tagcaaattt cttgatcaaa aaattcaact ttgctacacc gggacgtaac ggaaattacg
     1441 acaatgacag tgaagaaacc gttgtatcta acagcggaac aggaacagca gatcagcaag
     1501 tggtccagat cattcatttg ctgggaggaa aagaaaatat caaagatgtc gatgcttgta
     1561 tgactcggtt acgcgtaagt gtaaatgatc gagaaaaagt agggactgaa gaagcctgga
     1621 aacgagcagg ggcgatggga ttgatcgtca aagacaatgg agtccaagca gtttatggac
     1681 caaaagcaga tgtcttgaaa tcagatatcg aagatctgct gcaatcagga gcagagattc
     1741 cagagccaga aatggtttct acaggaaatg gacaagcagg tagtaagcaa tacttaggta
     1801 tagaacaaga actagtttcc gcagcttctg gagaagtgat tcctttatct gaagtaaaag
     1861 atccggtatt ctcacaaaaa atgatgggtg acggctttgc tgttattcca acatcaagag
     1921 aagttgttgc gccgatcgct ggaaaagtga caagtatttt ccctagcaaa catgcaattg
     1981 ggctggaaac gaaagatggt atcgaagttt tgatccatat gggaatcgat acagtccaaa
     2041 tgaatcagcc agcttttgag attgctgtca aagaaggcca agaagtagga acaggaacga
     2101 aattagctga gatggatctt gatgtgatcc aaaacgaaga aaaagatcca acgatcatga
     2161 tcgtatttac aaacgataaa gtagaagaag tagtgatcaa gcaattaggt acagtgacag
     2221 ctggtaaagt gatcggctcc attaaaatgt aaggagaggt tgtgaaaaag ctgtcctgca
     2281 tcaagtcata agaacagcca aacaaaaata gctcctccta tttttcgttt ggttgggctt
     2341 attaccgaga agcagcagtt tgaatactgt ttagtggaga ggtcgtgaaa aagctgccct
     2401 gcaccaagtg ataaaaacag ccaaacaaaa aatagctcct ggtccattga gcttattact
     2461 gaggagttag caggagaaca ctatttgctg gagaggttgt gacaaaaatt ttgtcacaac
     2521 ccttttgttt cgaaatgaag acaaggagaa aaccaatgaa attcatgaca ctcaacacgc
     2581 atagctggct tgagccagaa ccagagaaaa aattgcaaga attagcagat aagatcctgt
     2641 tagaagatta tgaagtgatt gctttacaag aaatcaacca attactggaa tctgaagaag
     2701 tagaacctgc gaagctaatg aagttctgtc cggtaaagaa tcaagtacct atccgtaaag
     2761 ataattttgc ctaccgttta gttcagcttt taaaggaaca cggaaaagag tatttttgga
     2821 gttgggaaat gagccacatc ggttatgata aatatgaaga aggaaatgca ctgcttacga
     2881 aaaaaacatt ggaaagtcag gtccttaccg tctcccaatt tcaaaagaaa gaagattatc
     2941 agacacgcaa gatcttgatt gggaaaacaa agatagatga tcaggacatt tttgtctcga
     3001 gctgccactt ttcttggtgg acagataaaa aaagtggctt ttattttgag tggaaaaact
     3061 tggaaaacta ctttttggaa actcgcgtac cacttttttt cctaggtgat ttcaataatc
     3121 cagtagactc tcaaggttat tatactgtaa gagaaagttg ccttcttctt caagattcgt
     3181 atgttgtcgc gaatgaaaaa ggaaaagcag ctactgtgga aaagaaaata gatggttggg
     3241 aacaaaatac agaaaaattg cgtatagatt ttattttcgt ccctgaagga atgcaggtta
     3301 aaaaatatca acggattttt gatgggatcg atagtccaat catcagtgat cactacgggg
     3361 tggaaatcga attagatgtg aacgaataaa aaatataaca tataacataa aaaaactgat
     3421 taaaataaaa ctctccaaac gattataccg tttggagagt tttttgatcg gatgtattta
     3481 tcccttttgc ctaagcacgt aaaagcttgt atctatgata aataaataaa catagcaagg
     3541 taaaccagcc aatcaaagta agagaaagaa tgaaatagaa atagattggc gtttgataat
     3601 gaagcgtgag ctcgttgatt cctttttgct gcttcacgat tggggtcccg atatctgtta
     3661 cttcataatc agtcaatgtt tttccatttt gctgtagcac agtccgatcg tagacgataa
     3721 cagggatatg aacagttttg tccccagtgt ttttccactt gattgtcaaa agaccgcctg
     3781 gatgtgtttt tataaaagtt gtttcgtttt ctagtatttc ttcgttatac cggtcgtatt
     3841 tattgtcttt tgtctcctta tagattggca gatagtcagg tgtggttttt tggaaaacga
     3901 acaagctggt agacatatct ttgctgaaga atgttttcct tgcttcttcc ggcgtatcga
     3961 atagataagt atgtcgactg acaaatgttt cattttccca cttttccaga tgctggtata
     4021 agttctggat cgtctgtgct gaaaaaataa gaatgccaaa tatacttaaa agacggaacc
     4081 acttttgttc agaccactgg ttcagtgaaa gggcaaggta aagtagaagc aatagcgtaa
     4141 atggcacgaa aaatcgaaat ggaaactgga tcaaactaat aaaaggcaca tttttttcga
     4201 tcaatgtcgt ccatggaatc aagctagtag aaagaaaaaa gaaaacaaga cttgtgtaca
     4261 acacgatttt ttggaataag gtgttctttt gttgaaagaa aataaaaagc aaaaaacctg
     4321 ataatatcaa tataggaaag gccttagggt aatggagcca ataactgcca tccagatcta
     4381 tcgccttttg actcatttct ctgttaacaa agggaggaag aatcttattc ccactgtaaa
     4441 caatagctaa tcctgcccaa atatttgctg tcagaacaaa ataaagcaag accgacttaa
     4501 tcagttgaag cagtgctttt cctttttcag tctggttaaa aaagtaataa agataaaaag
     4561 ggatatacat caaagctaac atgatcgaag acagtaaatg gatctgtgtc ataagtccca
     4621 ctgagacagc gactttgata acagggaact ttttatgaag gacgaaatca gcaataggga
     4681 tcatacataa aggataaaaa caagtccccc agctagtgaa tccttggttg atagaccagt
     4741 attgaataga atacgtactc atgaataaaa cggctactgg tacacttacc caatttcgaa
     4801 cattgacata tctcagtaaa atcagcatag aaaatcctga aataagataa agaatgaaat
     4861 ttgaaagaat ctgataatga aaccagctac ctgcaagcaa cacgatcaat ccttgtaaat
     4921 aagcgaaaaa tgggccgtaa acggcattca cgatcctgcc actttgctgg aatccataaa
     4981 gggaaggaaa ataatggaaa tccccatatt taatttgttg cgcggcatca tagaaccgat
     5041 tgtaatggaa tggggcatca gcacccaaaa ttagtttatg tgtgaataaa tgaggcatga
     5101 gaaatgccaa agcaatgaaa aaaattgcta aaaatgggcc atattttttc aattgtcgaa
     5161 ctcctttgca aactttagat aaattcatca tatatcctat gcgagaggta ggcaagaaat
     5221 tagaaaattc atgaaaatat ttatgaattt tacaagaaaa acataagtga aagttctaag
     5281 gagagaggat gcgacataag ttttgttgca tctccttgtt ttgaaacaga atttttctta
     5341 atactcgaaa caaagtagct ttttcatgac ctctcggtaa acggtgttca atcagctgct
     5401 tctcggtaat aagctcacta gcctagaaaa tatgagaagc tattttctga ctagcgctct
     5461 tattactcaa agcataacgc agttttcaca acctcttcct taaacggtgt tcaaaagctt
     5521 gcatctgcgg taataagccc aacccaacaa aaaatatgag tagctatttt tttgggctgt
     5581 acttattact tgatgcagga cagctttttc acaacctcat cggctttcat tttctatttt
     5641 tttcctgaac ccatataata aaagaaagaa caaaaaaact tgcaagggag gacacatgtg
     5701 aagcagagta agcaaaaaca gtctcgtcga cttttgtttc gtttgttttt attaatatgt
     5761 atcgtcgtag ggttactgat cgctttatat ccattttatg tggattcttt aaatagtttt
     5821 attgatcaaa aacgtatcga acaagtacaa aaacggaccg cagctgaaat tgaagtgcag
     5881 cgaaagaaaa tggaggagaa aaaccaacga ttaactgacc aaggattcaa tcctggtact
     5941 gatcctttca atgaaaagaa cagaaatgaa agtacaacat ccagccaatt agaggaatgg
     6001 ctgatcggaa gtatcaatat acctaagatc caattaaata tttcacttta tgatcggctc
     6061 aatggtagga tccttgaaaa tggagcaggc gtcttgcaag gaacctcttt cccgcttggt
     6121 ggaaattcga cgcattcagt gatctctgct cacagcggtt tacctaatcg acggttattt
     6181 accgaattag atcgtttaga aaacggagac acgtttattt tgactgtttt gggggagaaa
     6241 ttggcttatc aagttaaaaa tatacaagtg gtactgccag atgacacgtc tgttctaacg
     6301 atttcagaag gagaagactt agttaccttg cttacatgta cgccgtatat gatcaacacg
     6361 catcgtttgc tagtgacagg acatcggatt ccttataacg aatcggtaaa aaaagaagaa
     6421 ggaaaaggaa atcaggaaag aaccatgcgg caatggttta ttcttgcagg aacgataata
     6481 gctgtagtca tactgttagt gtttatcgga cgactaatct atcagtatag attaagtaaa
     6541 aaagtgcttg atttttcatt tattattagt gattcgtcag ggaaaccggt aaatggtagt
     6601 tcattcattt tgaaacataa gaaaaaaaca cttactagaa atggtgtgcc gttttcagta
     6661 caaagcgacc atcatgggaa agtcaagctg gatcaattgc ctggtggaac ttatcgaatc
     6721 gtttcagctg atgatcctaa agttgctgtt tcttttggta ttagaaaagt aaaacaagag
     6781 aagatatact tttttgaggg gcgaaaactt gtaaaagagc ttcaaaaaaa tggtttttat
     6841 tttaaaatca atgaataagt aaggagagaa aaaatgatcc gcacaacaac aaaattgtct
     6901 gccaaagaac tggaatctgt tctatcgatt tggctgcatg ctaacttgga ttctcatgaa
     6961 ttcattccat caacttattg ggagaatcaa gtagaatctg ttaggaaagc tttaccagat
     7021 gcagtgattt acttagcaga aatagatcaa gaaattattg gttttgctgg ggtgattgat
     7081 cagtttatcg caggaatctt tgtgaaaagg gaataccggc aacaaagaat cggtacgcaa
     7141 ctaatgaaaa aaataaaaga aaccaataaa acactgactt tgacagtcta tcaaaaaaat
     7201 caatcagcta ttcatttcta tctttcacaa ggttttacaa ttcaaacaca gcaaaaagat
     7261 gaagcagtaa atgaggagga acttgtgatg aaatggaatc aaaacgctga tttttgatga
     7321 acagaataac gaaataatgc tcccagccta attttttgct gggagcatta tttcattttg
     7381 aagtcactaa tttaactgtg tgaatatgca gttgttacaa tctttgataa acggtgttca
     7441 atcagctgac cctcggtatt agctcaaaaa acggcaaaat atgaggaact atttttccat
     7501 ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctcttgata aacggtgttc
     7561 aaacggctgc ttctcggtaa taagcccact agcctagaaa atatgaaaag ctattttcta
     7621 actagcgttc ttattactca aagcataacg ctgatttcac aacctcttga taaacggtgt
     7681 tcaatcagct gaccctcggt attagctcaa aaaacggcaa aacatgagga actatttttc
     7741 cgtttctttc tctaatactc aggtcagaac gctgatttca caacctctct ttcacaacct
     7801 tattttactc gtttataatt ttcatcgaag tattttccgc tacggatatc atctggcatt
     7861 ccatcgtatg gttcttcgct aataacatct tcattgtcta tacctgcatc acccatataa
     7921 gtgatcgtag caaattcagg aacaagtaat tttggataag tttcatattt ttcacgagct
     7981 gcttccattt ctgcgatatg ttcgttttgg aaagtaacag tgatttctgg atgttcttca
     8041 acccatttag ggaaaaagat cattccgtga agtttctttt catttggcat aaagtccaat
     8101 gccacgtctg tccctgttgg ctctgtccaa gtgattttga atacgccgtc cgtcaattta
     8161 acgatatcag cttcttggtc ttttacccaa cggcctttga ccattccgcc atggatacgg
     8221 taatcaacag tatggtcatt tttggcatac cattcatatt cccaaccatt gtcatacgta
     8281 tagataaagt gagttccaat aaaatcttcc aattctttaa attctttc
//
LOCUS       DAYYHX010000122         8155 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000122 DAYYHX010000000
VERSION     DAYYHX010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.123"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..1458
                     /locus_tag="ACHA6Y_001981"
     CDS             4..1458
                     /locus_tag="ACHA6Y_001981"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFM8988065.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            1534..2694
                     /locus_tag="ACHA6Y_001982"
     CDS             1534..2694
                     /locus_tag="ACHA6Y_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFM8988066.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            2807..3814
                     /locus_tag="ACHA6Y_001983"
     CDS             2807..3814
                     /locus_tag="ACHA6Y_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8988067.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            3838..4863
                     /gene="iolG"
                     /locus_tag="ACHA6Y_001984"
     CDS             3838..4863
                     /gene="iolG"
                     /locus_tag="ACHA6Y_001984"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HFM8988068.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            4880..5770
                     /gene="iolE"
                     /locus_tag="ACHA6Y_001985"
     CDS             4880..5770
                     /gene="iolE"
                     /locus_tag="ACHA6Y_001985"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFM8988069.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            5793..7388
                     /locus_tag="ACHA6Y_001986"
     CDS             5793..7388
                     /locus_tag="ACHA6Y_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFM8988070.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIACAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            7404..7718
                     /locus_tag="ACHA6Y_001987"
     CDS             7404..7718
                     /locus_tag="ACHA6Y_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8988071.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
BASE COUNT         2432 a         1538 c         1947 g         2238 t
ORIGIN      
        1 taaatgacaa gatttttaag aaattatatt gatggtcgtt gggtcgctag tcgctcaaca
       61 gagtttgaag aagtaatgaa tccggctaca gaagaagcgc tggcgaatgt accgttgtct
      121 agcaaagcag agacagccga agcagttaaa gctgccgatg ctgcgtttca aacttggaaa
      181 aaagttcctg ttcctgttcg cggacgttat ctgttcaagc tgcatcagct tttaacagat
      241 cataaaagtg aattggcgcg tttgatcacg ctggaaaatg ggaaatcatt gtccgagtca
      301 ctgggagaag ttcaacgagg aatcgaaaat gtggagcacg cagcgggaat caccaattta
      361 atgatgggag attctttatc aacagcggcg actgatattg agatcacgaa ttatcgttac
      421 ccaatcggag taatcggagg gatcacaccg tttaattttc caatgatggt gcccttctgg
      481 atcttcccga tggctctggc ggcaggaaat acggtggtgc tgaagccttc agaaaaaaca
      541 ccattgttga tggagaaaat tgttgagctg gttgaaaaag caggctttcc tagcggtgtt
      601 tttaatgttg tctatggtgc ccacgatgtt gtcaacacat tgctgcaaga tccactggta
      661 aaaggcatca gctttgtagg atcaaaaaat gtcggtgaat acgtttaccg tgaaggaaca
      721 aaaaatctaa aacgggtaca agcgttgact ggagcgaaaa atcatgttgt tgtgctgaaa
      781 gatgccaata ttgatcgggc agtcaaggac cttattggcg gagcttttgg ttctgctggt
      841 gaacgctgca tggcagcctc cgtcattgtt ttggaagagg aaatcgcgga tcagtttgtt
      901 gaaaaattta aggctgcggc aaaagcgatc aagatcggca atggcttgga cgaaggggtc
      961 tttttaggac cagtgattcg aaaagatgca cagcagcgga cctttgacta tatccaaaaa
     1021 ggggtcgatg aaggagcgaa actggtacta gatggtcgcg aaaacattcc agaaaaaggc
     1081 tattttgttg gaccaacgat ttttgataat gtcacgatgg atatgaagat ctggcaggat
     1141 gagatttttg ccccggtcgc atcaatcgtg cgggtaaaag atttgatggc agccgttgag
     1201 ctgacgaatg aatctgaatt tgcgaatggt tcgtgtctgt ataccaacaa cgcatcagcg
     1261 attcgctact tcagagaaaa tgttgatgct gggatgctgg gggtcaatct tggggtgcct
     1321 gcaccgatgg cttggtttgc cttttcaggc tggaagcact ctttctttgg tgatttgcat
     1381 gccaatggca aagacagtgt tgagttttat acccatcgga aagttgttac agcacgttat
     1441 gcgcaaagtg agatttaaag atttgcttcg aagctctgtt aaagagcttc gaggattctg
     1501 ttataggcta taagccagag agaggttttt tctgtgaata ttcaactgaa aatgcctgcc
     1561 aatttaagaa ttggcggcgg aacaatccaa tttcttatgg aagaatcaga acaactaggc
     1621 aacaagccgt taatcattag tgatacggtt atggaacaga ccgggcatct tcaaaagatc
     1681 aaggagctgt tgggaaaaac agatgatcaa ttagcggagt atacagacat tcaagccgaa
     1741 ccgacgcaag aaaatgttta tgatgcgctg aagctttttc agaagcatca atgtgatcat
     1801 atcattgctt ttggtggcgg cagctgtctg gatacagcca aggtggtagc cgttttggct
     1861 gctaatcaat taagtctcga agcgattcat gcaagaaatt ttgatcagct gcaagcgggc
     1921 attccgctga ttgcagtgtc aacaactgct ggaactggtt cggaagtaac cgatgtggca
     1981 gttattacca atacaaaaac agcagtcaaa atgatgatga aggatctcgt tttcatgcca
     2041 cagatctcaa tcgttgactc tgattttacg aaaactgtac caccaaaagt aaccgcggca
     2101 acgggagtcg atgcgctatg tcacgctttg gaatcttatg tttcagtcaa agccaatgaa
     2161 gtaacgcaac tattttcact ggaagcgatg aagaaaattt tgaacacgct ttctcaggca
     2221 tatgaaaata gcgacgacac tgaagccaga gaacaaatga gcttagcttc aacggaggcg
     2281 gggattgcct tttcgaatgc gtcagtcaca ttgattcatg gaatgagtcg accggtgggg
     2341 gcactttttc acattcctca tgggatatca aatgcgatgt tattgccggt cttttttgaa
     2401 ttctcaaagg atgctattca gactgagtta gcgacgatcg ccagatttat ttatccggca
     2461 gccgaagatt tatcgctgga agaggctgcg gattatctga ttaaaaaaat ccaagagctc
     2521 tgtgagcagc tgtcgatccc aacgattcga gattatggta ttgatcagca agcgtttatg
     2581 gcagccttag acaaaatggc cgcggatgcg ttgaccagcg gaagtccgca aaataataaa
     2641 aaggtgccta gtcatagtca aattaaggaa ttatacgtac aagcttatgg gtagtaaaag
     2701 gcggagcaaa gagagggctc taccttttaa gaataaaagc tacacaggaa taattttgta
     2761 agcgcatact atgtgtgctt aaacaacaga acagggggat aagtaattga gagtaaaagt
     2821 aggcgttatc ggaacaggtg caatcggcat cgagcatatc gacaggatca atcagaaaac
     2881 acaaggggct tatgtgacag cagtctcaga tatcaatgta aaagctgcta aagaggttgc
     2941 caaacgtgtc gatgctaaat tctttgaaac cggagaagca ttgatcgctt ctgaagatgt
     3001 ggatgtcgtt gttgtgactt cttgggaccc gacccatgag cagtatgtgt tagaagcaat
     3061 caagcatggg aaatatgttt tctgcgaaaa gccattagcg atggatgcag caggctgccg
     3121 ccggatcgtt gatgcggaag ttgcttatgg gaagaaattg gttcaggttg gcttcatgcg
     3181 ccgctatgac cgcggctaca ttgaactgaa agaagcaatc gaatcgaagc aatacggaga
     3241 agcattaatg atgcattgtg cccatagaaa tcctagcgca gatgaaaact acacaacacc
     3301 gatggcgatc agcaacaccg cgattcatga gattgatgtg cttcgctggt tgctaaatga
     3361 agattatgaa tctgttcaaa tgatcttgcc gaaaaaaacc agcaactccc atgccgattt
     3421 gcatgatccg cagctgctga tctttcaaac aaaatccggt gtaacgattg atttggaaat
     3481 ctttgtgaat tgccgctttg gctacgatat taaatgtgat gtggttttcg aaacggcaga
     3541 agtaggaatg acggacccag cctatacacg aattaaatcg gaagaaaaaa attacacacc
     3601 tgtttcacca gattggcaga cccgcttttt ggatgcctat gattatgaat tcaagctgtg
     3661 gatcgattcg atcaaaaatg atgcggttgt cggtcctacc gcttgggatg ggtatgtagc
     3721 agcgatcacc atgatggctt gtcatgagtc tcgtgaaaca aaaacaaaag taatgattca
     3781 aatggatgaa cgaccagcac tatacaatga gtaattctca gaaaacggag gtatttcatg
     3841 aacaacatta gagtaggcat cgtcggttta ggaagattag gaaaagtaca tgcacaaaat
     3901 ttagcaacag gtgtcagttg ttgtgagctt acggctgcgt gcagtttagt tgaaacagaa
     3961 ttagcattcg cacagaagaa attaggcgtc aagcatactt tttccagcta tgaagaaatg
     4021 gtcaaaagtt ccgtgatcga tgcagtcttt attgtttcac catcaggctt tcactgtgac
     4081 caagtccgct tggcgatgga aaatggcaag catgtcttta ctgaaaaacc attaggcctt
     4141 gaagttgaag atattaaaaa gacccaaaaa gtcatcgaag agcatcctga acaggtcttc
     4201 atgttaggct tcatgcgccg ctatgatgag tcgtatcagt atgcgaagaa aatggtagaa
     4261 gatggcgaaa tcggcgaaat cacactgatt cgttgttatg ggatcgatcc gagcgaaggc
     4321 ttggaaagct ttgtcaaatt tgccatgaat aacgacagcg gcggcttgtt cttagatatg
     4381 gcgatccatg atattgattt ggttcgctgg tttaccggcc aagaagtcga tcaagtgtgg
     4441 gcgattggga aaaatgcggc gtatccagaa ttggatcagg ctggtgagct tgaaactgga
     4501 gccgcaatga tgagcctgag cgataaaaca atagctttac tagttgccgg cagaaacgca
     4561 gcccatggct atcatgtcga aacagagatc atgggaacca aaggaatgct gcgggtagcg
     4621 caagcgcctg agaaaaatct tgtgacgatc atgaacgatc aagggatcat ccgtccatgt
     4681 tcacaaaact tcccagaacg attcagagaa gccttcattc gtgaagccca agaatttgtc
     4741 tcatgtattc aagaaaaacg tcaaccagcg gttaatgcag aagatggatt gcaatcaact
     4801 aagatcgcgt tggcttgtca agaatcattc gatacgaaga gtttagtaca actaaaaaaa
     4861 taagcaggag tgtgagaata tgaccaaaca aattcaatta gccatcgcac caatcgcgtg
     4921 gaccaatgat gatatgcccg agcttggtag tgaaaatacc tttgaacaat gtatcagtga
     4981 aatggccctt gctgggttca ccggaacaga gattggcaac aaatatccaa aagatccaga
     5041 ggtgttggaa caatatttga atattcgcgg cttaaaggtt gccagtgcat ggttcagtgc
     5101 gtttctgaca actaaacctt ttgaagaaac tgccgaagcc ttcaaagcac atcgggattt
     5161 cttacatgcg atgggcgcta aagtcatcgt agtagctgag cagggtcata gtatccaagg
     5221 gattatgaca gcgccagtct ttgatgataa accagttttc actgatgaag agtggaaaaa
     5281 actggcggaa ggattagaaa aattagggga tttggcccac gaaaaggata tggagatcgt
     5341 ttaccatcat cacatgggta ccggcgttca aacgacggaa gaaatcgatc gtttaatgga
     5401 aatgactgat ccagacaaag ttaaattatt gttcgatacg ggacatttgg tcttttctgg
     5461 tgaaaatcca atcgcggttt acaaccgtca tcaagatcgt atcaaacaca tccatttcaa
     5521 agatattcgt caagaaacgg ctgaagaagt agaaaaggaa aaagacagct tcttaaacgg
     5581 cgtgaagaaa ggcgtcttca cggttcctgg tgatgggatg attgatttca ctccaatctg
     5641 gacagcaatc catgccagcg actatgaagg ctggatcgta gttgaagcag aacaggaccc
     5701 ggctaaagcc aatccatttg aatatgcagt caaagccaga agctacattc gagaggtcac
     5761 cggtctttaa tctattgaaa aggagctaga cgatgaatct tttttctata atcagtttcc
     5821 tagtgattgt catctgtgta tggttatttg cctattctag atccagaagt gtcgatactt
     5881 ctggatcaga agggttcttc atgggagggc ggagcttaac tgctttgcca atcgccggca
     5941 cgatcatcat gacgaacctg tccaccgaac aaatagtcgg acaaaatggt caaagttatc
     6001 atgcagggat ggaagtaatg gcctgggaag taactgctac gattgcgatc gtagctttgg
     6061 ctgtgatatt cttgccaaaa tattttaaat acggcatcaa tacaatctca gatttcatcg
     6121 agattcgtta tgatacggtt accaaacgaa ttatttcgat cttatttatt gtgacctata
     6181 tgacttcttt cttacccgtg gttctttatt ctggatcgct ggtgttcaat aagatcttcc
     6241 atattgatga gctgctgggc gtccagccga tcgtcgcgat cataattgta gcgatggcaa
     6301 ttggtattgt cggcatcctc tatctgctga tcggcggctt gtcgttaagt gccaatagtg
     6361 actccattta tggagtcggt ctgctggctt gtggtctgct ggtcccaatt ttaggcttaa
     6421 tgaagcttgg tgacggcagc tttgttggcg ggatcgaaca cgttgtggta aacacgccat
     6481 ggctgcttaa ctctgtggga tcgatcgact cggctgttgt tccatggccg acactattta
     6541 cagggatgct gtttaacaat ctgtatttct ggtgtaccaa tcaaatgatc gtccaaaaag
     6601 cattgtccgg caagaattta gctgaggcac aaaagggcgc tttcctagtc ggcgtcttca
     6661 aaatcttagg tgcgttattc cttgtctttc cagggatcgt cgctcgcaat atttttggcg
     6721 atagtttaat gtcaaatcca gataacgcgt atcctttttt agtcagtgaa gtattgccta
     6781 aaatgatctt tggtattttc gccgctgtta tctttggcgc gatcttatca tcatttgcgg
     6841 gagctttgaa cgcgacatcg accttgtttt cactggattt ttataaaccg cttatcaata
     6901 agaaagcaaa tgataagcaa atcgcttgtg ctggtaaaac agtaacggtc gttgtgggga
     6961 ttatctccat tatcgtagcg ccattcattt cttttgcacc ggctggattg tatcagttcg
     7021 ttcaagaatt caatggacta tacaacatgc cgctgctcgt gatcattctc tttgcctttt
     7081 attcaaaaaa agcaactgct tttggggcaa aaacgacgat tgccgtgcat atcgtcttgt
     7141 atgcactatc aaaaatgctt ttccaaaata tccatttcct ttatgttttg acggcattat
     7201 tcgttctgga cattatcatt ttgatggtgg tttccaaatt gaaacctgat ggagaatttg
     7261 tgttgaactc attcgataca aaggtcgata tcgagccttg gaaatacacc aaggtgactg
     7321 ctatagcatt ggtagtcatt gttattttaa cttatgtggc attttcacca attggctttg
     7381 caggctaaaa acaggagggt attatgcata aggatctatt gattatttgc gagaccggaa
     7441 tcagtgcgtc gctgctcgta tcaaaaatgc ttgaaacgat cagagccaaa aatctatcgt
     7501 atgagataga ttatgcaacg atccgaaacg ttgaagaaaa actgaattat aaagactatg
     7561 acgttttact tttaacgccg caggtcgcac gttctgagga aaaagtaaaa gaaatcatca
     7621 aaaaggaaaa atgtaccgct cagatcgttt atatcaaaca agaagatttt cgttatatga
     7681 atattgaaaa catcattgca agcatcgaag aagattaacg agaatctttg attattgagt
     7741 gtcgaggagg tggtatatga aaagatggca tgttcgcccc gatcacccca atatgaaggg
     7801 cggacttatt agaaataccc aatcttgtat ttattcaata gggtaagcgg acctcaatag
     7861 ccagctcatc cccaaaaagt cgaatggaga cacttaaatg agtgtaccta ttcgactttt
     7921 tgagactcta agtcaaatcg tgttgttctc attaaatcac tgaaaggaaa tgcttcggcg
     7981 tttccttttt tctttttgtt tttcttcgtg ttttgaaaat tacttactat ttactatacg
     8041 agtagtttca aataattttg acatcctaat cttcaacaac agaaaaaaat ctaaattata
     8101 aaatttaggg ttgactaaaa tataattttt gtataccata tgatcgtgat aatca
//
LOCUS       DAYYHX010000123         8146 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000123 DAYYHX010000000
VERSION     DAYYHX010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.124"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            349..1212
                     /locus_tag="ACHA6Y_001988"
     CDS             349..1212
                     /locus_tag="ACHA6Y_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8988072.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1341..2963)
                     /locus_tag="ACHA6Y_001989"
     CDS             complement(1341..2963)
                     /locus_tag="ACHA6Y_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8988073.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3212..3733
                     /locus_tag="ACHA6Y_001990"
     CDS             3212..3733
                     /locus_tag="ACHA6Y_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8988074.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3919..5649)
                     /gene="spxB"
                     /locus_tag="ACHA6Y_001991"
     CDS             complement(3919..5649)
                     /gene="spxB"
                     /locus_tag="ACHA6Y_001991"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM8988075.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5814..6632
                     /locus_tag="ACHA6Y_001992"
     CDS             5814..6632
                     /locus_tag="ACHA6Y_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM8988076.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6735..6932)
                     /locus_tag="ACHA6Y_001993"
     CDS             complement(6735..6932)
                     /locus_tag="ACHA6Y_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988077.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7101..7679
                     /locus_tag="ACHA6Y_001994"
     CDS             7101..7679
                     /locus_tag="ACHA6Y_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8988078.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7774..7911
                     /locus_tag="ACHA6Y_001995"
     CDS             7774..7911
                     /locus_tag="ACHA6Y_001995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988079.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2456 a         1451 c         1607 g         2632 t
ORIGIN      
        1 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
       61 ctattttttc cgtttttttc tctaatactc aggtcaaaac gctgatttca caacctctga
      121 ttcacggtgt tccagaagta gcttctacgg aaataagccg aaatttcaca aaaatttgag
      181 aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctct
      241 tattttgatt agaacttgcg aaaataaacc ggttaaagtg tgtctagcgc aagagatttg
      301 ctataataga cgataagggt aaaatgtaac gagaaggggt tgaaaaatgt gtatcaaatt
      361 gtaacagatt cttgttgtga tctgccatac catgtattag caaaagaaaa agttgatttt
      421 ttatcaatga atctccaatt gaatgatcaa gagttagtag atgacctcgg aaagacattc
      481 aattatgacc gctacatgaa tcagctgaaa aatggtgcga tgcctacgac atcacaaatc
      541 aatgttggcc gatatttaga atttttccgt tcttatgcgg aaaaacagat tcctgtttta
      601 tatgtggctt tttccagtgg attaagcggc tcttacagta gtgcgcttca agcggttgaa
      661 ttgctgaagg aagaatatga agaccctgag atctatgttt ttgatacaaa agctgcaagt
      721 attggggaag ggcttttagt attggaagct gtaagattga aaaaagaagg taaaagcatc
      781 gaagaaaccc tagagtggct ggaaagtaat tatttgaaag tccattcatg ggtaacagta
      841 gacgatctga aatatctaga acgaggcggt aggatttcca aagcaagtgc tactcttggc
      901 agtctattga gtgtaaagcc tattattacg gtggataaag aaggaaggct gacgaatgtt
      961 gacaaagttc gaggaagaaa caagtcgatc cagacaatcg tcaatgagac ggtaaaaaca
     1021 atcgttgcac cacttgatca aatcgtttat gtggcttacg ctggggatct agagagcgcg
     1081 gagaaggcaa aacggctttt ggaagagcag ataaagatga aagatgtgaa gctttatccc
     1141 ttaggaccaa cgatcgccag tcacacaggc tatggctgta tcgcgatttt ttcgatggga
     1201 atcagtcgat aaaagcagta actcattctg taaaaaagcc cagaaattga ttgtaacaaa
     1261 aacagcgact cttaaaagat agatgagcat cctatctctt agaggtcgct gtttttttat
     1321 tttttaagct aaataggcca ttatgcccat aactcgttca attgtttttg aatatctgca
     1381 ttttccaaaa attcatcata tgtcgtatca gctcgatcga tcacaccttt atccgaaacg
     1441 gcaatgatgc ggtttgccaa tgtttggata aattgatggt catgtgacgc aaataagatt
     1501 gagccagtaa atgccatcaa gccgtcattc aatgctgtga ttgattctaa gtctaaatgg
     1561 ttcgttggat catctaatac aagtacgtta gctttcgaaa gcatcatttt tgaaagcatg
     1621 caacggactt tctctccacc agataaaaca ttcactggtt tcaatacttc ttcaccagaa
     1681 aacaacattc ttcctaagaa gcttcttaag aaggtgttat catcttcttc tttacttgca
     1741 tattgacgca accagtctaa aatcgttaga tcagtatcga attctttcgt tgtatccttt
     1801 ggtaaataag aattacttgt tgtcacaccc caacgtactg ttccagtatc tggcgtaatt
     1861 tcacccatga taactttgaa tagcgttgtt gttgttatat cattttctgc gacgaaagcg
     1921 accttgtctt ctttgctcaa attaaaagat atatcatcta aaattttctt tccatcaatc
     1981 gttacactta cattttctac ctgcaataag tcgtttccaa tctcacgttc aggtttgaat
     2041 ccaacgaatg gataacgtct agaagaaggt tggatatcgt ctaaagtaat tttttcaagc
     2101 atttttttac gtgaagttgc ttgttttgat tttgatgcat tcgcactaaa gcgggcaata
     2161 aattcttgga gttctttgat ttgttcttct tttttggcat ttgcgtttgc ttgaagttta
     2221 gccgctagtt gactagattc caaccaaaaa tcataatttc ctacataaag tttgatttta
     2281 ccaaaatcta gatccgccat atgtgtacat actttgttca aaaagtgacg gtcatgggat
     2341 acaacgatca cagtattttc aaaattgatc aaaaattctt ccagccaatt gatcgattgg
     2401 atatccagac cgtttgttgg ttcgtctagc aaaagaacat caggtttacc aaatagtgtt
     2461 tgtgctaata acacttttac cttttgtcca ccagttaatt cactcatttg ctgatggtgc
     2521 aattcttcag ggatgttcag accttgcagt aaaacagctg cttctggttc agcttcccat
     2581 ccattaagct cagcaaattc tccttctaat tcagctgctc gaataccatc ttcgtcagtg
     2641 aaatcctctt tcatgtaaat cgcatctttt tctttcagca cttcatacag acgcttgtgt
     2701 cccatgatga ctgtttcaag tacagtatag tcttcatagt cgtaatggtt ctgtttcaat
     2761 acggccatcc gttcatctgg tcccatagta acggaacctg tggaaggctg cagatcccct
     2821 gataacactt taaggaacgt tgatttacct gcaccatttg caccaatcaa gccatagcag
     2881 tttccggggg taaatttaat attcacatca tcaaatagtt tgcggtctga aaagtgcaaa
     2941 ctcacatcat ttacagtaat caattttttt cctcactttt atcattatta gttttgacaa
     3001 gtcttataga attatatagg ggaaaggtaa aagattcaag gcaatcgaac attctttgtg
     3061 tttcacgtga aacagaatga aaaaaataaa aataaaagcg gagcgaaaat agctactata
     3121 ggatttatta gtttgcaaca gcataatttt tgacgttatt tataagaggg agtactataa
     3181 aactgtaaaa gaaatgaaag aaggattgaa aatggatgaa aaaagaacca tcgactgggg
     3241 ctcattgatt ttgggaattt tatttgtact tgtctcattg ttatcatttc aagaccctgt
     3301 cggaaattta gttgcaattg tagtagtctt tgcgattttt gcctttatta aagggatatt
     3361 tgaactgttt gtacggaaca gattaaaaga gctaacagga tataaaggaa agatgccctt
     3421 aataatcggg atcatcgata ttctagttgg cgtctttttc ttattcaata ttggtgcagg
     3481 agtcgcagca ttgccttttg tttttgcagt atggtttatt gctgattcag ttttagcgtt
     3541 atttactgct gacttggcaa gaggagtaag tgaaggatat tattggttta cgatcatcgt
     3601 caatatttta ggaattttat taggaatttt cttattgttt aatccaattt cttctgcgtt
     3661 gacattaagc tttttagttg gattctattt tatgttattt ggtatcacac atatcgtcta
     3721 tgcatttaga taagtaaaat caaacaaata aaaaagccgg cttccgatta cagttttact
     3781 gttgtaattg gaagccggct tttttatcat ggacaaatat gcacgaggtg atttttaggt
     3841 gtcgagacaa atttatgcaa aaatgtgaga agctatatac acaaatttta tcttaatact
     3901 cgaagcatag cgcagttgtc acaacctctt ttcatataat tcattcaata ctggcaaatc
     3961 atggacttca tatttttctt tgaatgcttg gatttcttct tcagaatatt ttcttggatc
     4021 taaatgcaga tcttctactg gcaatgggcg cttgttattg atcttcacat cgattactac
     4081 aggacgatct gattttgcag ctgcatcaaa ggcaaatcga agttgcgagt attctgtgac
     4141 tgtaaagcta tttgccccga gcgcttctcc aactttaccg aaatctgcat cttctaaaaa
     4201 tacaccaaat ttcttttgtt ccgtatcttc ttgttctgct tcgatgaaac caaaagaatc
     4261 attcgaaaag acaacattaa taattggaag ctgatatttg acttgtgtaa tgatatcttg
     4321 catgaccata gcaaacccgc catcaccact taaggtaaat acttgttttt ctgggtagct
     4381 tagttgtgcg gcaattcctc ctggcacacc attacccata gttgcgaacc atccagatgt
     4441 cgtatgtttt tgtttaccgt tcatattcaa taaacgaata gaatggatag tcgtgttacc
     4501 gacatctgtg acgaaaatcg catcgtcttc agcaatacga ttgatttctt tatagacagc
     4561 ctctggacga atcggttctt cctctcgatc ttcaaaagat ttcaaccaat ttacccattt
     4621 ttctttattt tcttgatttg ctaggtacca gctatcagca gggcgagcat cccctaactc
     4681 gacaagtctt tgcaaagctg tgtttgcatc accgaggatc gacaaagatg tttcatggcg
     4741 tcgtccaaat tttgacgcat cgatatcgat ttggataaat tcagcagttg gattaaaaaa
     4801 tgcacgtcca aatgggaagt cgctaccgac aaataagatc agatcggctt ctgccaatgc
     4861 ttcatttccg ggtttagtag cgacacgtcc agcaaaacca agaaagtttt cataatcatc
     4921 aggaataatt ccttttgcta gaacggaagc gacaacagga actgaaaaat gttctgagaa
     4981 ttctttgatt tgcggaaatc cgtttcgcgt tccttgacca acataaagaa ctggtttttt
     5041 tgctttttca agataaggca aagcagctag caaatctttt tcttctggta atatcacact
     5101 tgttttgtga gtgtgagcgg tggagaaagg ttgttcgtca atttcttcaa aaccaagatc
     5161 aacagggata gtcacgatag ccacgccttt atgctcatag gcggctttaa tggcttcatc
     5221 tactacatgc ggaagacttt gtggtgtcat gactgtacga ttgtatacgc taacatctgc
     5281 gaaaatagga ttttcattta gttcctggaa agagttatag ttcatggatg tagaagcaac
     5341 ttgtcctaag aacgctacta gaggcacatg gtccatctgt gcatcataca aaccattgat
     5401 caaatgtgtc gcaccaggtc ctgcagagcc gaagacagcg ccgactttcc ccgttaattt
     5461 tgcatctgca gcggctgcga gtgcgccgac ttcttcatga cgaacttgaa tgtagttgat
     5521 ttcggttttt tcttgatata gcgcatccat aattgaattg aaagatcctc cgggaatgcc
     5581 gtaaatatgg tcgatacccc agctttcaag aacttttacc attgcgattc ctgcattgat
     5641 ttttgacatg gtgtttcact cctttagcaa tgatgataca taaatttttt gtatctagag
     5701 tatagaactt tttatcgaaa atcccaacta attcgtcttt tcacaaaaat gtagtcatgt
     5761 ttttgggaat atgctagagt agtacataga tagattcaag agaggggaat ggcatggttg
     5821 aatggctatt gagacttgaa ccttggcagc aagcacttgc tgggacagct tttacttatt
     5881 ttatgacagc tcttggtgcc gggttggttt ttttctttaa agaaatcaaa aaagaagtgc
     5941 taaacttgat gttaggattt gcttctggtg tcatgattgc tgcaagtttt tggtctttgt
     6001 tagatccggc tattacaaaa gcagaagaaa atggagatat tgcttggcta gttgtaagta
     6061 ttgggtttgg tttaggcggt gtgtttttat atatggcaga taaaacactg ccccatatgc
     6121 attttggacc acaacatgaa gcagaaggat tgcctaccca tttaaaacgg acgatcttgc
     6181 ttgttttttc tattacattg cataacattc ctgaaggttt agccgtgggg gtagcattcg
     6241 gtgcagcagc aactgcggat aatccgacag ccgctatatt agccgctgtt tcggtggcgc
     6301 taggtattgg tatccaaaac ttcccagaag gagcagctgt atcaatcccg ttgcgacaag
     6361 aaggtctgag tcgtaaaaag gcctttgttt acggacaagc ttctggaatc gttgaaccaa
     6421 ttgctggagt gattggagcg ctactagtaa cgaaagtgga gctgctgctt ccttatgcac
     6481 ttgcttttgc cgcaggagca atgatttatg tcgtggtgga agaattgatc cctgaagcac
     6541 agcagacatt gtccagcaaa catcattttg cagtttttgg cgtcatgagt ggatttatca
     6601 ttatgatgat tttggatgtg gcattaggat aaataaaaaa aatatatatg atatatcaaa
     6661 tagggccgcg aacttatatc gcgaccctat ttttttagta agttatatca taatgaagtg
     6721 aatggtatca tacattaata atagtttttt ctatgtgtat ttcctttgat ccagcgaaca
     6781 agccagatga taagtgcgat tgggattaga actttgaccg caaaccataa aagcccgctt
     6841 aggacactga caaaaagact gatgacgaag ctagctgcaa ttagcactaa aagaaccatg
     6901 aagatattta agaacatatt tttttgacgc atacaaaacc ctcctcttgt tcattcttta
     6961 ctatagaaaa agtataacag ggattgttta aaaacacctc caactgaaga ctgattttta
     7021 cttcatctac ttaaatgatc aaacaattgc tttttctctg gtttatcatc taaaatagag
     7081 cggaaaagga ggagaatatc atgcctaaaa atcgtcttga agcatttaca gatgctgtaa
     7141 tcgcgattat catgactatt ttagttttag aacttcatga acctaacgga gacacgtggg
     7201 aggcgctcgc tgcattaggt taccgatttt ttatctatat tgtcagtttt atcattttag
     7261 cgatttattg gaacaatcat catcatttat ttcaagttgt acataagata aatggacggg
     7321 tcttatgggc gaacagtttc tttgtttttg cgctttcgct ttttccgttt gcaacttcat
     7381 ggatcagtga gtacatcaat agcctggctc cagaaatgac ttatggtttt gttaccttga
     7441 ccgctaatat tgcgtattat atcttggcaa aagaacttgt caaagcaaat aaaggccacg
     7501 agaaagcatt gaaggcgatg ttgaataatt acaagaaatc gtatctatcg attggtttga
     7561 atgtgttagg aattgtctta gggtatttag tagcgccagt agccgtgctg atcgcaaaca
     7621 tcgtgattct agctgcttgg atcatcccaa gtcgtaccat cgaatcacaa tatcggtaaa
     7681 atgatccgtg tttaaaattt aagagaaaaa tatagaaaaa ataaataaac gatcaaatat
     7741 ccgagtgata ctagtattca aagagggatg cagatggatc aaaaaagaat tcatgtagga
     7801 ctgattacta gttttgtcac gatgcttatt tatttggatc aaggtcattt gacttggaca
     7861 gtagttggtg tcgtaggatt tttgacatta ttcacagcaa taaaaaaata aacatcttta
     7921 tgtaatctga tgttctatct atttaagaaa agcatgttta cagcagtatg attctgttgt
     7981 aaacgtgttt ttatttgtgg aaaatttaat ttttagaaaa gtgtatcgaa aaatagatag
     8041 ttagtttttc cacagtggat aacttcgtaa gagataaaaa taaaaaatta gtcaaagaag
     8101 ggcgtataga tttcttttga taggagatat acaacagaaa tgaata
//
LOCUS       DAYYHX010000124         8051 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000124 DAYYHX010000000
VERSION     DAYYHX010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.125"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1112
                     /locus_tag="ACHA6Y_001996"
     CDS             <1..1112
                     /locus_tag="ACHA6Y_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8988080.1"
                     /translation="KLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAP
                     VLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSL
                     ASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSI
                     VGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANY
                     ISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVF
                     NDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFL
                     GVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1240..2616
                     /locus_tag="ACHA6Y_001997"
     CDS             1240..2616
                     /locus_tag="ACHA6Y_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8988081.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3017..4597
                     /locus_tag="ACHA6Y_001998"
     CDS             3017..4597
                     /locus_tag="ACHA6Y_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM8988082.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4795..5019
                     /locus_tag="ACHA6Y_001999"
     CDS             4795..5019
                     /locus_tag="ACHA6Y_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM8988083.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(5224..5544)
                     /locus_tag="ACHA6Y_002000"
     CDS             complement(5224..5544)
                     /locus_tag="ACHA6Y_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8988084.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(5692..7926)
                     /locus_tag="ACHA6Y_002001"
     CDS             complement(5692..7926)
                     /locus_tag="ACHA6Y_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8988085.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
BASE COUNT         2473 a         1429 c         1608 g         2541 t
ORIGIN      
        1 aaaaacttag acagtctaaa ctaatgtttt ggtcgcttga attattgatc atagctgcat
       61 tgatcatgat ttcatcagaa ataaacttta ttttcgaacc gatcgggaca tttttttcca
      121 cgttatttgc acctgtactg attgcaggct ttctttacta tttattgaat cctattgtca
      181 agctgttgat gaaagcaaaa atcaaaagaa tatgggctgt cgcaattgtg ctgctccttt
      241 tagttgcagc aattatttgg attttactta gtgtcatccc aagtctagta cagcaaattt
      301 cctcattggc atctaatatg cctgatttca tcaaacaggt agaaagctgg ttaaaagagg
      361 tagccgaatt gcctttgttc aaggaagtag atatcaataa atattttgag caattagaca
      421 tttcttacgg gacaatcatt cagcaattcc ttagtggagt atccaatagt ttaggttcga
      481 tcgttggaac gatcgcttcg gcaaccgtag taatcattac agcaccattc atcttgttct
      541 atatgttgaa agacggagaa aaactagtgc caaacatcca acagtttttc ccagaaaaaa
      601 gaagaaacca aattgtggag ttattggggc aactgaataa aacattggct aattacatta
      661 gcggacaagc aatcgaatgt ttattcgttg gtaccttcac atttttagga tatatggtaa
      721 ttggtgtgga ttatgctttc ttatttgggg tcattgcggg attgacaaac ctaattccat
      781 atttaggacc ttatctagga ctagcacctg catttctagt aacagtcttc aatgatccgg
      841 taaaagcatt actttgttgt gtggttgtgc taatcgtcca acagttggat gggaatatca
      901 tttatcctaa tgtaattggg aaatcactga aaatccatcc attaacgatc attatcgttc
      961 ttctagtagc tggaaatatt gccggattgc ttggtatctt cttaggtgtt cctttttacg
     1021 caatctgcaa gacgatcatt tcctatataa tcaaaatcgt caaagaagac aagcaaaatg
     1081 aaaacaagaa gaaaattgct acgacctcgt aggtagtttt agtacacttg tttttattgc
     1141 aaagaaaatt gctttgtgat accattttaa cgtggctata gtgccacgtt tttttctatc
     1201 gtaaactaaa aattaatttt ataaggagag aatgcgatta tgaatgctga ccctgagagt
     1261 cagtcgctgt tagcgcagat tttattattg atcgttttga cattgatcaa tgctttttta
     1321 gcagcttccg aaattgctgt tgtttcgatc aataagaatc gaatcgagca aaaagctgat
     1381 gaaggagatg tgaaatctca aaagctttta aaaatattgc agaatccaaa taatttccta
     1441 tcgacaatcc aagtaggaat cactttggtc aacattttgt ctggtgcttc actagctaat
     1501 actttatcgg aacggttagc tccggtatta ggtggcggca cagcggcaaa aaatattgca
     1561 aatatcattg tgctggctat tttgacctat gtatcgattg tttttggaga attgtaccct
     1621 aaacgtattg ctttaaataa atcagaagaa gtcgcaaagt tcacttcagg gctgatacgc
     1681 ttgattggtg tagtagctaa gcccttcgtc tggctgcttt ctgcttccac aagtttattg
     1741 gcgcgtatca cgccgatgac ttttgatgat gaagattcaa aaatgaccag agatgaaatg
     1801 cgttatatgc ttgaaaacga aggcgtattg aataatgaag aattagaaat gctccaaggg
     1861 gttttttctc ttgatacgaa agttgcccga gaagtcatgg tcccacgaac ggatgcattt
     1921 atgattgata tcaacgacac tgtggaagaa aatgtgaatg aagttttatc cgaaaactat
     1981 tcaagaatcc ctgtttataa cgaagacaaa gataaagtag tcggaatcct tcatacaaag
     2041 aatttactaa aagctgccca taaattcggc tttgataatt tagatatcaa gaaaatcatg
     2101 caggagcctt tattcgttcc tgaaacggtc tttattgatg atctgttata tgaaatgaaa
     2161 aagacgcaga atcagatggc tattttatta gatgaatatg gcggcgtcgt tgggttagca
     2221 acactagaag accttttaga agaaatcgtc ggagaaatcg atgatgaatc agatgaagtt
     2281 gagaatcttt atgaaaaaat cgatgaacac gaatatatca tccaaggacg tatgctgatt
     2341 gatgagttca atgaagcttt tgacagtgat ctgcatatga gtgatgtaga tacgatggct
     2401 ggttatttga ttacagcatt aggcatgatt ccggatgaag gcgaaaagtt atcgtttgat
     2461 gtcgataata ttacgctggt ttctgaagaa atggaaggtt ctcgaatcct taaaatccgt
     2521 gtcatttttc atgatccgga agaaacagaa gcggaacctg aagaagaacg aagatacttc
     2581 agaaaagaat tcgaagatga cgagccaaga agataattgg aaacttggtt atgaaagacg
     2641 tgttgcttga ataaacaagc aacacgtctt ttcttttagt cagctgacaa aaaaatattt
     2701 gactcttatg gtttggtggc tttttcattc gctgaatcca tgctatactg attcagttgt
     2761 agtagttgac aaaggttgtg aaaaagctat cctgcattaa gtaataagag cagccaagca
     2821 aaaacagctt ttcgtatttt tggcttgggt gggcttattg ctgataggct gtctctagaa
     2881 cgccgtttac tcggaaaaag agactgctac acggcttttg tcacagccct atatgaaatt
     2941 gtctttcttt aagagatcgg acaatctgtg cctagagaaa atataaataa ttgaatggaa
     3001 gaaatggagt taggttatga ataatccaaa attaaagcaa caagtggaca atcgcagaac
     3061 ttttgcgatc atttcccatc cggatgctgg gaaaacaacg attacagaac aattactatt
     3121 gtttggtggg gctatccgtc aagccgggac cgtaaaaggc aaaaaaacag gtaattttgc
     3181 caaatcagac tggatggaaa tcgagaaaca acgtgggatt tctgttacta gttctgttat
     3241 gcagtttgat tatcaaggga aacgagtaaa tatcttggat acaccaggac atgaagattt
     3301 ttctgaagat acttatcgta ctttgatggc cgtggatagt gcggtcatgg taatcgatag
     3361 tgcaaaaggg atcgaagcac agacaaagaa attattccaa gttgtaaaaa aacgtggcat
     3421 cccgatattt acttttatca acaaactaga tcgtgatggt cgtgaaccat tggaattgtt
     3481 agaagaatta gaagagctat tagatatcga atcttatccg atgaattggc caatcggtat
     3541 gggtaaaggg ctagaaggtc tttatgatat ctaccataat cgtgtagagt tttatcgtcc
     3601 ggaaaattat caggatgaac gattagtaga attggatgaa gatggactgc ttcccgaaaa
     3661 tcatcctcta actgaaaatt ccttatatga acaagtctta gaagaagtag aactaatcaa
     3721 agaggctgga gatgcattta atcaagaaaa aattgctcgt ggcgatcaga caccagtatt
     3781 ttttggttct gcattgacta acttcggtgt acagactttt ttagagactt tcgttgaatt
     3841 ggcaccagct ccttatggac acaaaacaca agatgatcag atggtaagtc cttatgagga
     3901 agaattttca ggtttcgtat tcaagatcca agcaaatatg aatccagccc atcgtgaccg
     3961 aatcgctttt gtccgaatct gttctggaac attcgaacga ggaatggatg tctggttgga
     4021 acgtacaaat aaaaaactga aattaagcaa tgtcacacaa tttatggcag attcgagaga
     4081 aaacgtcgaa aaagcagtag caggcgatat catcggtgta tatgatacag gcaattatca
     4141 gattggggat acattgttcg aaggaaaact aaaagttgct tatgaagaac tcccttcatt
     4201 tactccagag ctgtttatga aagtgactgc aaaaaatgtc atgaaacaaa aatccttcca
     4261 caaaggaatc tatcaattag ttcaagaagg agcgattcag ctttataaaa catatctaac
     4321 ggaagaatat atcattggag ctgttggtca gctacaattc gaagtattcc aatatcgtat
     4381 gttaaatgag tataacgccg aagtaatcat gtctccaatg ggacacaaaa tcgctcgctg
     4441 gatcgatcca gaagatttgg atgagaagat gagttcaagt cgcaatatct tggctcgtga
     4501 ccgtttcgac caaccgttgt tcttattcga gaaccaattt gcagaacgct ggtttgcgga
     4561 taaatatccc aatgtaaaat taaaaagttt gatgtaaaga agtcttgaca caagctgccc
     4621 tgcatcaagt aataagaata gccaaacaaa aacagcctct catattttgt ttagagccgt
     4681 gacggatttt ccgtgacggc tctttttact atattaaaat catagaaatg tctaatcaaa
     4741 aaaatataaa aattattttg cgtacatatg attaaagaaa ggagagtgtg acacatggaa
     4801 acagaatggc aaagacgtga aagaaaaaaa cagaaaaaag aagagatgtt acgattatgt
     4861 cgattattag atgtaaaaga ctgggacaaa aacgagcaaa ttgcacaaaa agtacgatca
     4921 atcatccata aagaggaaaa agttaaaaat aaagacacgc gtagaactga aaatgaaaag
     4981 agaaaagtac aaaagaaaag agaaaaatca aaaagctaga aagaaaaaga ggctgagaca
     5041 gaagcgttta actccaagaa ataagctgga attcacaaaa atttgaaaag caattttcgt
     5101 gaatttcagc ttatttccga aggagttgct tctgctttcg ccgtttatac gtgtttagag
     5161 cgtgagacaa aagtgttctt tacttttgtc ccacgctctt taattattat gttcaaaatt
     5221 tgtttacacg gctaatgtag cgcctaattc atgttttgga atcgagattt caacaaggcc
     5281 tgccaattcg attactttga tttcatcctc gtggtaaacg gcgtctttga ttttatctaa
     5341 gaaataattt ttagcatctt cgatttcttt cggctggatt ttacgtttgt cattgatcag
     5401 cagaatttca ttatgttcag gggcttcagt gtagacaata gtggtatttc cggctgtgta
     5461 gaccttgact agatgggcat cagtgttttc aagctggttt aatacaagac gggaatggct
     5521 gtttgttaca tttaccaatt tcatggcgct cacctctttt atgtattttt aattgcttgt
     5581 ttctcttatt ataatttctt ttttataaaa tgaacagtat tttgactttt ctgaaaaaag
     5641 aatgagacgg catttctcaa agaaataccg tctcacgcta taaaactgga tttattcagc
     5701 cgtttcttcg gctgattctt tagccaaacg ttctggttta gaagtaacga tgatttttcc
     5761 atctttatcc agttttgctt tcaactcatg gatagaaggg tgatcaagat aaaattcagc
     5821 gataccatct tcgatttgtt cttggatcgt gcgtcgaaga ggacgagcac ccatagatgg
     5881 atcatagcct aagtcaacta atttttcttt gacattcgta ggaacttcga tgtgaagttg
     5941 ctgtgctgcc agcatttggt tgacatcgtc tagcattagt gtgacgattg tcatcaagtt
     6001 ttcttttgat aatgcactga attcgatgat cccgtcaaaa cggttcagaa attctggtgt
     6061 aaagtagttg tttaattggt tcaatacaga acgagtaaca ccttcgcgtg ccgcaccaaa
     6121 accaacgttt gcttctactt taccagttcc ggcattactt gtcataatga tgatcgtatc
     6181 tttaaagctg accgttcttc cttgtgcatc tgtcaatcga ccatcatcta agatttgcaa
     6241 gaacatatgc aaaacatctg gatgagcttt ttctacttca tctaacaaga caagactgta
     6301 aggatttctc cgtacttttt ctgttaattg accagcttct tcgtatccga cataacctgg
     6361 aggagaacca atcaatttag agacactatg tttttccata tattcagaca tatcgaaacg
     6421 aatcattgaa tcttgtgaac cgaatagttc gtaggcaagt tgtttcgcaa gctctgtttt
     6481 acctacacca gttggtccaa caaataagaa ggagccgatt ggacgattct gtttgtttaa
     6541 tccaacacgg tttcggcgaa tcgctttcgc tactcgatct acagcgttat cttgtccgat
     6601 gacatgagct ttcaagtcat ctgctaagtt tttcagttgt gtctgttctt tttcttttaa
     6661 ttcacccact ggaatacctg tccgttgttc aacgattttt tccatatctt tttcggtaat
     6721 cacaggtgtt tcctcttcag tgagctgacg ttctttcatt ttttgaagtt tattgatctg
     6781 atcgcgataa taagctgctt tttcgaaatc ttcttctcga gaagctaaaa ctttttgttc
     6841 ttctgcttct tgcagttttt tctcgattgt tttaggatca acgatttgga tcgtcaagtt
     6901 tttcttagaa cctgtttcat ctagcaaatc gatggcttta tccggcaaga agcgatcttg
     6961 gacgtaacga ttggataaag ttgcggctgc ttcgatggcc tcatcagtat attttacatg
     7021 atgatagtct tcataacgtt tttgcaaacc tttcaaaata ctgatcgttt cttcgacagt
     7081 tggttcatct acccgtacag gttgcatacg acgttctaaa gcagcgtctt tttcgatgat
     7141 gcggtactca tttaaggtag tagcaccaac catttgcaat tctcctcgag ctaaagctgg
     7201 ttttaaaata ttaccagcat ccatatttcc atcaccagca gaaccagcac cgacgatttc
     7261 gtgaacttca tctatgaata ggatcacatt ttctgcttga cggatctctt caatcaattt
     7321 ttgcatacgt tcttcaaatt gtcctcgtat accagtacct tggactaaag atacgacatc
     7381 taaacgaatg acttctttgt ccattaattt ttgcggtaca tcaccatcta cgattttttg
     7441 tgctaaacct tcaacaactg ctgttttacc aacacctggt tctccaatca agacaggatt
     7501 gttcttggtt cggcgattta aaatttcaat gactcgtttg atttcgtcat cgcgtccgat
     7561 cacaggatcg atatcacctt gtcgtgcttg ttctgtgata ttgattccgt attctcccaa
     7621 taaaccttct gtattgcctt gaggtggctg accaccattg aagttgttgt ttccgccaaa
     7681 ttgtgttggt ggtgtttgct cataatttcc ttgctgttgc atttggcgag acatggaacg
     7741 gaaaagatcg tccaaactgc caaatccaaa tggatcattt tgggccatat tagtcagacc
     7801 tccattttgt tgatttttga ttttttgata acagctctga cagtaatcga gttgtgtccg
     7861 ctgaccattg acagtggcat acaagtgaat cgttgcctca ttcttgccgc agttttgaca
     7921 aagcatgaat cattcacgtc ctttcacaaa aatgggtaca tccatatttt acatctcgtt
     7981 tttcatgagg taaaagaata acccctagtt ttttaagaga tttatgaggt agaaaaggtg
     8041 aaatctctat t
//
LOCUS       DAYYHX010000125         7890 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000125 DAYYHX010000000
VERSION     DAYYHX010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.126"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1093)
                     /gene="queA"
                     /locus_tag="ACHA6Y_002002"
     CDS             complement(62..1093)
                     /gene="queA"
                     /locus_tag="ACHA6Y_002002"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM8988086.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1286..2461
                     /locus_tag="ACHA6Y_002003"
     CDS             1286..2461
                     /locus_tag="ACHA6Y_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8988087.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2465..3106
                     /locus_tag="ACHA6Y_002004"
     CDS             2465..3106
                     /locus_tag="ACHA6Y_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8988088.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3103..4020
                     /locus_tag="ACHA6Y_002005"
     CDS             3103..4020
                     /locus_tag="ACHA6Y_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8988089.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4017..4688
                     /locus_tag="ACHA6Y_002006"
     CDS             4017..4688
                     /locus_tag="ACHA6Y_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8988090.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5229..5717)
                     /locus_tag="ACHA6Y_002007"
     CDS             complement(5229..5717)
                     /locus_tag="ACHA6Y_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM8988091.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5918..6184)
                     /locus_tag="ACHA6Y_002008"
     CDS             complement(5918..6184)
                     /locus_tag="ACHA6Y_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8988092.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6184..6447)
                     /locus_tag="ACHA6Y_002009"
     CDS             complement(6184..6447)
                     /locus_tag="ACHA6Y_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8988093.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6968..7108
                     /locus_tag="ACHA6Y_002010"
                     /pseudo
     CDS             6968..7108
                     /locus_tag="ACHA6Y_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            7385..>7890
                     /locus_tag="ACHA6Y_002011"
     CDS             7385..>7890
                     /locus_tag="ACHA6Y_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8988094.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
BASE COUNT         2280 a         1649 c         1525 g         2436 t
ORIGIN      
        1 ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc ttttcataac
       61 cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct tcaatcgcat
      121 gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac atgactaaag
      181 tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat tcatagccag
      241 gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta aatttcgttc
      301 caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc ccgccatttt
      361 tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat tcactatgca
      421 tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac cctacatgta
      481 gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat tctttcgtaa
      541 aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag acagtttgat
      601 agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt tctcccaatg
      661 attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt ccgccgtgtt
      721 ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa
      781 ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt gtattcgtta
      841 gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa cgagcaggca
      901 gaactcgtgt attattcata actagcgcat caccaggctg taactcttca atgatttcat
      961 ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa cgagaatgat
     1021 cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt
     1081 ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct atcatgtttt
     1141 tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc taaataaaaa
     1201 aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat agttcttcat
     1261 tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa
     1321 aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg agttcatttg
     1381 ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca accgaatgac
     1441 tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa ttgatccagt
     1501 ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc ctcacatgac
     1561 gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa
     1621 taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta
     1681 cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc
     1741 gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa tcactcgtga
     1801 ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga tcgtatttgt
     1861 aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca tgagtgaagg
     1921 ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa acgatttcgt
     1981 agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga caagagtcgg
     2041 cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac aagaagcaat
     2101 caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt cgaatgtatt
     2161 aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg
     2221 cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc gtgatacctt
     2281 gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac agaacaaagt
     2341 cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga tttggggaga
     2401 agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta aggaggtctg
     2461 acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca
     2521 tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac cgcttggtgt
     2581 cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca gtgccttgca
     2641 gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg taggtaaatt
     2701 acctgcgatt atcgcactct ttatttattc attattgccg attttacgta atacgtacat
     2761 tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg ggatgactga
     2821 gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca tggctggtat
     2881 tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata tcggcgctgg
     2941 tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc tcattttggc
     3001 aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag gtctcttgga
     3061 acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg tatactaaaa
     3121 atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc tggtcttgcc
     3181 agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga agcacagatc
     3241 ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac cgcaatcatc
     3301 aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga tgcttcaatt
     3361 tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc tcctgaaaaa
     3421 gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata cggtcaaacg
     3481 tggttcccgt cctatggttt tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca
     3541 caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga attagtagct
     3601 ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt ccaagaaact
     3661 tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta tgacgcagtc
     3721 gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat cgccagttat
     3781 gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc tgctccagtt
     3841 gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa taagttaggt
     3901 cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa taatctagtg
     3961 gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga agggaagtga
     4021 agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag gaaaatggct
     4081 cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt gtcctctttg
     4141 cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa ttagcaaact
     4201 gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg atctccattt
     4261 taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt ttgtattccc
     4321 tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa aacatccttg
     4381 atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta gagttaccac
     4441 tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc atcgggataa
     4501 ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc cgaggaacca
     4561 atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca ttaatggcga
     4621 tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc agccgtacat
     4681 cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct
     4741 tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc cttatgggca
     4801 aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat actgccaacg
     4861 ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca ttttgccaat
     4921 ccattttccc atcactcgtc atggaattca taccacatga tctctaaata ctcgtctgct
     4981 tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat ttaagccttt
     5041 tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg tgactgccgt
     5101 atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat ccttggctaa
     5161 aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct aaaaccggta
     5221 cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg atccttcacc
     5281 agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa gacattggcg
     5341 taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat ggccgaaagc
     5401 gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt cgctttggat
     5461 acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc ttttccaaaa
     5521 gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt gtccattgct
     5581 tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt cttcgcctca
     5641 ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc tccaatttta
     5701 ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca acattccatt
     5761 cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta aagaaatcat
     5821 gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt tggggagact
     5881 ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt gtccaggcag
     5941 aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga tgctgtctat
     6001 tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg
     6061 gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc tttaaattcg
     6121 tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact gtatagttac
     6181 tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt cgtctttttc
     6241 gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc
     6301 tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac taataatctc
     6361 gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt tacgagcagt
     6421 gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata taattatagc
     6481 gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc aaacttttcc
     6541 tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca cgctcacttc
     6601 ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac gtatcatctt
     6661 tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac acgaaggttc
     6721 ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca cgaaagattt
     6781 gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca aaaatgattg
     6841 cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt agaccgttgg
     6901 gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat ggtcacttga
     6961 cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg taaaggaaga
     7021 agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt atgatcaaga
     7081 catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc atacgctgtt
     7141 cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa acagaccctc
     7201 tagcttcaat taagagctag agggtctgtt tttgatttta aaacggttct ataatatttt
     7261 gtgttggtgg aaaatcgaaa gcgattttct actaacactt ttttcttcgt ttcaactcaa
     7321 aaagagactc acttttggta aaatttagtc gaccaaaacg tcaccctacc aaaggagtct
     7381 ctcaatggat aatcatacta gaaaattact cggattaaca gataaacatt tattttttga
     7441 tgaagaatgg ctgattgaaa aagaatataa aggcgttcaa gcgcagttta ttaaagggag
     7501 attggatgac tcaccttccc attgtgaaca ctgtggatcg attcgtatta tccgcaacgg
     7561 ttcctatacg acacgcactc agatgttgaa agtcaaagaa aagttgacta ttttagaatt
     7621 aaaacgtacc cgttttctct gccatgattg tggacggacc ttttctgcga aaacggactt
     7681 agttgatgaa catcatcaac taacaaagga actgaagcag gcgattctca tggaactgta
     7741 cgaaaatcaa tcacgtaaac taatcgctaa gaagtacttc gtctcggatg ggacggtgac
     7801 acggatcttg cgtgaagcaa cgaagcatta ccagccacgt atgaacttcc ttccgacggt
     7861 gttgtgtatg gatgagttca aatcaatgaa
//
LOCUS       DAYYHX010000126         7814 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000126 DAYYHX010000000
VERSION     DAYYHX010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.127"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..791
                     /locus_tag="ACHA6Y_002012"
     CDS             90..791
                     /locus_tag="ACHA6Y_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8988095.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1400..2416)
                     /gene="tsaD"
                     /locus_tag="ACHA6Y_002013"
     CDS             complement(1400..2416)
                     /gene="tsaD"
                     /locus_tag="ACHA6Y_002013"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM8988096.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2413..2877)
                     /gene="rimI"
                     /locus_tag="ACHA6Y_002014"
     CDS             complement(2413..2877)
                     /gene="rimI"
                     /locus_tag="ACHA6Y_002014"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8988097.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2884..3426)
                     /gene="rimI"
                     /locus_tag="ACHA6Y_002015"
     CDS             complement(2884..3426)
                     /gene="rimI"
                     /locus_tag="ACHA6Y_002015"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8988098.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3410..4132)
                     /gene="tsaB"
                     /locus_tag="ACHA6Y_002016"
     CDS             complement(3410..4132)
                     /gene="tsaB"
                     /locus_tag="ACHA6Y_002016"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM8988099.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4323..5303)
                     /locus_tag="ACHA6Y_002017"
     CDS             complement(4323..5303)
                     /locus_tag="ACHA6Y_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8988100.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5327..6811)
                     /gene="galT"
                     /locus_tag="ACHA6Y_002018"
     CDS             complement(5327..6811)
                     /gene="galT"
                     /locus_tag="ACHA6Y_002018"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM8988101.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6823..7812)
                     /gene="galE"
                     /locus_tag="ACHA6Y_002019"
     CDS             complement(6823..7812)
                     /gene="galE"
                     /locus_tag="ACHA6Y_002019"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM8988102.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2181 a         1690 c         1389 g         2554 t
ORIGIN      
        1 ttgtttctaa tgttggtatg ttaaaacttt acaaattctt taaaaaaagt gacaataaat
       61 atagtgaaca ataaggagag gactttttaa tgcgtgttaa taaaatcgat tttcactttt
      121 caaaactcag atatcaacct gattttgaac atttctcgga tgaagagttc caacagatca
      181 aggaacatac tgttcttcgt tcttataaaa aaggacagat ccttttcgac gatggtgatc
      241 ccagacaacg gttgtatatc ttgaccaaag gactcgttcg tttagaacgt tatgatgaaa
      301 gtgcaactta ttactactat gactatgtga atacaaataa cctttttcct atgtcaggaa
      361 tgtttggaga agagacctac acttatacag ctcaagcgat gactgatatt gaaacattct
      421 accttcctgt ttcctactat gaaaaattag cacgaaaaaa tcccgaacag ttgatctata
      481 tgatacaaaa aatttctaaa gtagttgaaa tacatgaatt gcgtatccaa atcggattga
      541 cctctagcgc ctttgaccgg gtaaaacaaa atttattcat tttgaaagaa gaattgggcg
      601 taccaacaaa agatggtcat atcaccattc cttatccgat caccttaaaa gaattagcag
      661 tgaatagcgg cacgacacgt gaaactgctg gacaagtaat caaacaacta cgtgattccg
      721 ggctgttaga ttatgacaaa aagcgtttta ctttctgtaa gacagctact actccagcta
      781 tgagagaata acgcgaacaa aaataagcag tgaccctgat aaatatcagg gtcactgctt
      841 attttcctcc ctaaaacttc tgaaagatcc ggcagtttta gatcactgga caagactcaa
      901 agattttcta tgtgtgtttc agttatgtaa tattcactgt ttgcaaaaaa tactgttaga
      961 ttaactagat gaagcgtctt gtcctcatcc gatctagatt attcgatatt cctcgacaat
     1021 aaacgattac tcagtgaggg caatcagagg aacggataat gataaataag ctgtaaaaaa
     1081 tagcaaaata acaataatga ttaaaagtat gtaaaaaata gggctttgac aaaaacgtgt
     1141 cacagcccta tttcgattaa acggtgtgta cctgtcaact cttcggtcac aagtcataat
     1201 gttcataaac atgaggagct gttttttgaa attgttcctt gtgaatcaga gttaaacgac
     1261 aggcttacga actcttgatt aaacggcgtt caaaagcttg catacgcggt aataagaaca
     1321 gccaggtcaa aaacatgaga agccgttttt cctgggctgt tcttattact tgatgcaggg
     1381 cagctttttc acaacctctt tattcagata aaaccaagct tggatcagca ttcaactgat
     1441 agctagcaaa atttcttttt tgcatttcca catgtgctgc tgctccaatc attgcagcat
     1501 tatctccaca aagtcgaaga gggggaatca ctagttctac ctcagggagt ttagctgcca
     1561 aagcatcctg caatccttca cgcaaccctt gattggccgc tacacctcca gctataatca
     1621 gttgcttgac tggataattt tgacaagctc gaagtgtttt agtaatcaat acatcgatca
     1681 cacttgcttg aaagctagct gctaaatcat ttttgtctag atcttctcca cgctgttgtg
     1741 cattatgcac caaattgata aatgcacttt ttaatccgct aaaactgaaa tcataattat
     1801 cttcatggat catcgctcgc ggaaaatggt aattatcttt tccctgatga gctagctgat
     1861 cgatttcttt tccgcttggg taagaaagac ctaatactct tccaactttg tcataagctt
     1921 ctcctgctgc gtcatctctt gtctccccaa tgatttcata tgatccatct tcttgcatat
     1981 agactaattc tgtatgtcct ccgctaacaa gaagtgccat caatggaaac tggaatggtt
     2041 taaccagacg ggctgcatag atatgccctg ccatatggtt tacaggaata agcggcaatt
     2101 ggtgtgccca agcaaaagcc ttagctgcag aaataccaat cagcaacgag ccgacaagtc
     2161 ctggtccata agtgacagcg actgccgata aatcttctgc cgacacacca gcttctacca
     2221 gtgcatcttc caagcataag gtgatttgtt ctacgtgatg gcggctagct acttcaggta
     2281 ctacgccacc aaaacgttta tgactgttta tttgagaagc aacgatgtta gataagatct
     2341 ctgttccatt tctaacgacc gctacactcg tttcatcaca acttgattca atcgccagaa
     2401 tcaattcttc attcatttca tcgtctccgt ttttaacttt ctgctcataa tgatcgcatc
     2461 ttccgtcgga ttgcgataat atgatttccg tttacctaat atacgaaact tttctgattc
     2521 ataaagtttt cttgctgctt cattagatag acgtacttct aaaaaaacat gcagcgaatc
     2581 ttttatttgt tccgtaatca gtaattgtaa caaatgccgt gcatgccctt ttccttgttc
     2641 tgaaactgca acagcaatat ttgtgatctc gacttcatct attactttcg agtatccaat
     2701 aaagccacag atctggttat cttcagctaa aagcagataa tcagagtgag gctgttggat
     2761 gtctgccaaa aactgcattt tcgtccaagg tgaaccatgt ggataagcat tttcgctaat
     2821 catccatacg cattctgcca agtacgcttc atcaaaatct ttttttctgc aaatcaacta
     2881 atctcactca ttttctaaaa gttttatcat atccaacgca tcttcttttg tttcagtgta
     2941 atatcttttt ttgatcgttg ttgcttgaaa ccccaatttc cgataaagtc tttgtgcatc
     3001 ttgattactc atacgcactt ctaatgacat cgtctcacag cgattcatta tagcaaagtt
     3061 ctcgatttcg tcaataagca gcgaaccgat tcgtttacgc tgatatgccg gtcgaacggc
     3121 aatattcgtg atatggcaat ctaaaccgaa cgctcttgaa ccgataaaac caatcattct
     3181 tccattatca aagataccta gatatagatg aggaagcggt gacttcaatt ctgatataaa
     3241 ggcacttttt gtccatggca attcgccatt atagacttct cgttcgacat ccagcagctc
     3301 tttgatatct tctgtagtca attctcttag tacgtaacga cgatcgattt gttcgactgt
     3361 tttttctgta taaatcattt tatcttgaaa aaagcttctg gcatacgctc taaatttttt
     3421 caacataact ttcgtctcca ggtgtgtgcg tctccaacca tttttcttct gcttctactc
     3481 gttttaaata gcgaggaaga aaagcttgtt tctctgcaac tggttcaact aaagcaccta
     3541 attgagccac agttacacca cttggaatat cccatgctgg aactgtatta atcatcgcac
     3601 taggcaatat ttcctttatt tcagagaaga atttgtgaca atcactcccg acgaaataaa
     3661 tagcttcatt cagcttttgt agttgtgtga acaaatcaga agcagaaata tgtacatctt
     3721 caagtacagt ttctaatcta ttttctttcc agcggtatgc cccagcgtat acattttttc
     3781 ttcgagcatc aaacatgggc acaataatat tatccacacc gatgcaatta gccgcgatgg
     3841 ttttcagact agatactcct actagctctt tatctaatgt ataagcaatc gtttttgctg
     3901 tcgttacacc tatacgaagc cctgtataag aaccgggacc atcagaaacc gcgattcggt
     3961 caatgtcttt tggacttatt tgaagatctg caaacaattg gtcgattgca ggcatcagtg
     4021 ttacactgtg gttgcgtttg atatttgttt gtatttgtcc aagaatttgt tcgtcttcta
     4081 ccacaccgat cgccaaagtt tggtttgcag tatctattcc taatgtaatc atatatttga
     4141 tcctttctgc tatgacattc tcgccttcca ttgtagcata cttttttatt ctgaggatga
     4201 aaataccaaa ggcaaaacta gtacaataat acatgattat ctgctggcgg gcaaattttg
     4261 ccccttcctg aaacacaaaa aagctgcggt atcactccat tttcccacag cttttccatc
     4321 ttttatttcg ttgattctcg aataatcaac tctgtttcca caataatctt tcttgaaacc
     4381 ggtgctttgg aattacttaa ttgaagaatc gtctccactg ctagctctcc catccattcg
     4441 gtataaactt ttacagtaga aagcgagggc gttacatatt tcgctacact gacatcattg
     4501 aagcccatca cagaaatatc ttctggtacc actaaacctt tttcttgaat cgctttcagt
     4561 gcgccaatcg ctaatgcatc attagctgca aaaagcgcat caggaacatg cggattcgtt
     4621 tttaaatatt gcttcactgc ttgatatcct gactcgacag aaaaatctgc ttcgataatt
     4681 ggaccttcgt cgtagccttt ttctgccaaa acttgttgaa atgccataat tctgggatct
     4741 tttagccgct gatggttttt cttcgtatat tctgttccag caagcatagc gatatgttgc
     4801 gctttatggg agacaaagtg ttcaatgact ttttgaacac ttgacttaaa gtcgacaact
     4861 agagagtcga ttccttgaga agtgccgtca gaatctacca aaagcaaggc attttgggat
     4921 cctttcattt ctaataactg agcttcgtcg aattttccta gtgcgatcgt tccatctgcc
     4981 ggaagatcac taatggaatc ccacgtttct ttcaataaag taacgtttgc ttcttctgcc
     5041 tttttttcga ttcctaggcg aatagataaa taataaagat cttcaagctc ttccgtatcg
     5101 tcgtaccact ggatcaggcg aagcgtttgc ttagtatttg ctgcattttt acgatgtttc
     5161 gtataattta agcgttctgc tgcttcaaaa attttttgct ttgtggctaa gccaacggaa
     5221 agatcaggat catagttgag aactcttgaa acagtagcag gagaaactcc tgccaactgc
     5281 gcaatgtctt taattgttgc catttgtctc tcctcctttt ttacgtttat aaaatcgcag
     5341 taaatcgttc aaacgctgcc tgtccttttt catctcgctt gaatactcca gcgtcttcca
     5401 gtacacgtgc aaaaacctga cctacagatt tttgtacgac atcggtgacc gtatctttcg
     5461 tcaaatcagg atgttctttt tttaattgtt ctgcccacgg acgatgatat tcagccatct
     5521 ggttttcttc tcctaataag aaatgctcta cttctactag ttctggtttc aaacgaggtg
     5581 gtaaaacagc aagccccatt acttcgatca acccaatgtt ctcctttttg atatgttgga
     5641 catctttatg tggatgaaaa atcccatcag gatgttcttc tgaaacattg ttgtctcgaa
     5701 ggacaaggtc caactcaaag taattgtccc ttcttcgtgc aattggcgta atcgtatggt
     5761 gtggtgtacc atctgctgaa aaagctctga tagatactgt ttcatccgaa tatgctttcc
     5821 atttttccaa tactgagtta gctgcttcaa taagcaagtt tttctctgtt gattgcaaac
     5881 ggatcacaga catcggccat ttcacgactc ctgcgatgac ttgcggataa tcttctagtt
     5941 tgataagttt ttctatcggt gcttttgcca taggaaattc atgacgtcct gcctgataat
     6001 gatcatgaga aagaatcgat cctccaacaa ttggtaagtc agcattcgaa ccaacaaagt
     6061 aatgaggcaa gacttcagta atccgaagca gtctagcaaa tgtttccttt gtgattttca
     6121 tcgggcggtg ttgttctgat aaaatgatcg aatgttcatt gtaataagca taaggggaat
     6181 attggaatcc ccagctttca ccgtctagat tcattcgaat aatccggtga ttggtgcggg
     6241 ctggatagtt caagcgacct ctatatcctt cgttttccat gcaaagcatg caggtaggat
     6301 agtctacttt taccgcctcg cgttctgctg cgatttgttt tggatctttt tctggcttag
     6361 ataaattgat tgtgatttcc agttcaccat actccgactc tgccggaaat ttgatgtttt
     6421 ttgcgatggc tcttgttttg atgtagtcat ttttcttact taaattaaag aaatactcag
     6481 ttgcttcctc tgggcttttc gaatagtgct gtgcaaagaa tgcattgacg actgaaggtg
     6541 gcggtgtgag aaaatccatt aattgcgctt caaaaatctc ttgttctgct ggcaattctg
     6601 aaagaacacc attttctctt gccttagcga caagctgatc caataagtct acagattctt
     6661 ccttaactgg tcttacttca atgctttcta gagcatcttc tcctaccatc cctaaaagac
     6721 gattctgtaa atataagcgg tccatttcca tccatccgcc agcttcgatc gcaagtgtgg
     6781 caaaatcaca aatcgtttga ctgattgaca tatctcattc ccctattctt cataaccatt
     6841 tggatggctg acatgccaat cccatgctgt tttgataatc gctttgatat ctgtgtattc
     6901 aggtttccag cccagaatct ctctcgcttt ttcacttgaa gcgactaatc ggctaggatc
     6961 tccagctctt cttggtgcta tttctgctgg aatttctttt cctgtcactt ctctagctgc
     7021 ttctagcatt tccttgactg agtatccttt gctgctgcct aaattgaaga agttgctttc
     7081 atttccttct ttcaagtatt ccaaagctaa aatatgtgca gcaatcaaat cttctacttg
     7141 aacataatct ctgatacatg ttccatctgg tgtatcataa tcgtctccat aaacagccaa
     7201 ggcttttctt tgaccaagag ctacttgtaa aatgattggg acaagatgcg tttctggtgt
     7261 atgatcttct ccgatagatg catctgcttt tgctccggct acattgaagt agcgcaaagc
     7321 gacatagcgc atgccatatg cttggtcaca ccacttcatc attttttcca tcatcaattt
     7381 actttcacca tacggatttt taggattagc tggtgtatct tctgtgattg gagactcttt
     7441 cggctctcca taagttgctg cagtagaaga gaatacgatt ttattcacgt tgaactcgtg
     7501 catgacttca agtaagattt gcataccgta tacgttgttg ttgaaataca tcaatggttt
     7561 ttcgacagat tctcctacta aagagcttgc agcaaaatgg attactcctt caatcggttc
     7621 cttttcaaat acggaacgta gaaattcttt atcccggatg tctccttcat aaaaatgagc
     7681 atctggatgt actgcttgtt tgtgacctgt gagcaaatta tctaccacag ctacttgata
     7741 ccccttttga actagctggt ctacagcgtg cgaaccgata taaccggctc cgcctagtac
     7801 taaaattgtc ataa
//
LOCUS       DAYYHX010000127         7778 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000127 DAYYHX010000000
VERSION     DAYYHX010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.128"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..339
                     /locus_tag="ACHA6Y_002020"
     CDS             22..339
                     /locus_tag="ACHA6Y_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8988103.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            332..940
                     /locus_tag="ACHA6Y_002021"
     CDS             332..940
                     /locus_tag="ACHA6Y_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM8988104.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            995..1786
                     /locus_tag="ACHA6Y_002022"
     CDS             995..1786
                     /locus_tag="ACHA6Y_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM8988105.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            1924..2412
                     /locus_tag="ACHA6Y_002023"
     CDS             1924..2412
                     /locus_tag="ACHA6Y_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8988106.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            2463..3335
                     /locus_tag="ACHA6Y_002024"
     CDS             2463..3335
                     /locus_tag="ACHA6Y_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8988107.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            3511..3873
                     /locus_tag="ACHA6Y_002025"
     CDS             3511..3873
                     /locus_tag="ACHA6Y_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8988108.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            3882..4634
                     /locus_tag="ACHA6Y_002026"
     CDS             3882..4634
                     /locus_tag="ACHA6Y_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8988109.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            4648..6450
                     /locus_tag="ACHA6Y_002027"
     CDS             4648..6450
                     /locus_tag="ACHA6Y_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8988110.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            6727..7767
                     /locus_tag="ACHA6Y_002028"
     CDS             6727..7767
                     /locus_tag="ACHA6Y_002028"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8988111.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         2615 a         1281 c         1656 g         2226 t
ORIGIN      
        1 ttgagaatag aaggtgacgt aatgcacatc cagattccaa ctgttcttct tgatggtact
       61 gtgctagcag cattaagcaa ggaagatatg tatgggtatg ccttaacaaa aatcgtacaa
      121 gaaagtctct ctgtcagtga atctacacta tatccggttt tgagaagatt gaaaaaagaa
      181 ggatatcttg agacctatga tgaacctttt gaaggcagaa accgtcgata ttactcgttg
      241 acaccaatgg gaagtcgtca tttagaagaa attattgatg aatggattgt ttttcgagat
      301 tctttagacc ggttattgga ggagagcaca aatgaatgaa tatttgaatg aagttgtgga
      361 tcatcttcaa gaaattcctg aagacgatcg ttttgatttg atccagtatt atgaggaata
      421 ttttttagat tctggaaaaa cattagatca gatcattgat gaatatggta caccaaagca
      481 gtttgcctta aaattaaaaa tcagttactt ttcagaagaa gaacaagcag aaaacgagtt
      541 agtgaaaaat ccaaaaagac aaattcgcct gatttggtta atcattatcg gtttatttgc
      601 ttctcctgtc ctcattccac tagcactggg gtttatattg gttattgggg gactattggt
      661 tgctttacta gcgctcattt ttagtatcta tgttttatgt atcagtatct taggtgttgg
      721 cctattcatg atcatctcag gaatcagtgt gtttggacaa tcgattctga gtggattatt
      781 ctttatcggt gtcggtctgt tcgccacagg aagcgcagta tttatttcgc cactgttgct
      841 gaaaggaaca aaagggcttt tttatgtcat gatgaacttt gttaaatgga tgggacgacg
      901 ttttattaca aaaagagatc tgcattcagc gggcgtttga aggaggacac ataaattgag
      961 attgaaacat tttttgatta ccggtattgg tctgatgctt actggtggcc tcctagcagg
     1021 tgcaacttat acattaggcg ctcaaaagag tctgacttgg gataatggtc ctcggttgat
     1081 cgatatggtt actgagacaa aaaaaatcaa ggatgcaaac aaaatcgtca tccatgcaga
     1141 aaatcaaaca gtttcggtca tacgcggatt ggattttgag ataaaaacga cttatgataa
     1201 gacagaaaag ccagaaataa aggagaaaaa tggcgtgttg actgtgaatg cagtcaaaaa
     1261 atcaacaggt gcaatcatta gtttagaaga cacaagtgca caaatcacta ttgttgtccc
     1321 tgcagatcat gaattatctg aattgacaat cgaagggaat aatagtgcaa ttgatatcaa
     1381 cgaagtatca atcaaaaaag tgaatactaa gacacatgat ggctgggtat cgttcaatga
     1441 atttacagga ggaacaataa ctgcgaaaga tgaattaggc tcacttcgat tagatgaggc
     1501 caatgtggat aacttgatcg tcacaggtga aaacagcaac ttccatctag aagacagttt
     1561 gatcagcaaa gaaggaaaag tcatcctaaa cggtggcaat ttatctttgc atgagatgga
     1621 aaacagtggc tatactatcc aaacgtctgg tgaatcttat attgaaaaag actaccagcc
     1681 aataacaaat actttgttag aacaaggaca gaaaaagcta aagatcactg ctaaagacac
     1741 atcgatcaat attacgacgg atcaagagga caatgaaaca gattgatcct gtactcaact
     1801 ggatatttta agaaaaaaca gcaaagatcc ttccaatctt tgctgttttt catcgcttaa
     1861 cgcacataac tatagtaaaa taagaaagaa aatatctaaa aagaaaacag ggtgaaagag
     1921 tatatgaagg tagaagaagg tcaaagagaa aagttaaaaa cagaaatgga aagattacat
     1981 acttatatca ctcaactaag ccaaaccttc tatgatccag acaaagaaaa agtgatggtt
     2041 aattacccga acaattcaga aggtcgccaa ttagagcaag tgtaccatga agttttcaaa
     2101 catttattga cggtgaagaa agagctggat tattattctt taccgattat cgatacagga
     2161 atattgaagt atgatgggaa gaaggaacga tttatcttta aatctgttcg ggaagatttg
     2221 cctctttcag cagggatgga tctagaggtc ttggtagaag actattttac agaagaaaag
     2281 cactgggttc gaacaaaatt ggattattta ccgcaggcag ccggagggac acaggcaagc
     2341 ggttggtaca tcactgaaga caaagagtta gaactagaag gtgtaatggc gcgtattcga
     2401 aaaaaaaatt aaaaaagtaa cattagatat ttattgaagg ataaaggagc gcaggaagta
     2461 gcatgagaga aaaaagagca ttttatatca atggttatgc agggattgtc ggattggtcg
     2521 tattagcttt agttggattg tttttcttct atttaggtat gtggcaagcg aaagtttggg
     2581 cacttttttt aagtattttt ctttggttga tcacgttatt gttgttaagt tcggcaactg
     2641 tagtcagtcc taatcaagca aaagtaattt tgttttttgg acaatattta ggtacgattc
     2701 gagaaaatgg ctttttcctt acgatacctt tggcacaaaa aatgactgtt tcgttgaaag
     2761 ttcggaattt caatagttct gttttaaaag tcaatgattt ggatggaaac cccatcgaaa
     2821 tctcagcagt agtagtcttt aaagtcgttg atacagcaaa ggcattattc gatgtagcgt
     2881 attatcaaga ttttgtagaa atccaaagtg agacagccat tcggcatatc gctagccaat
     2941 acccgtatga tacattcaat gagaatgatt tgacgcttcg aggaaacacg acggcagtgt
     3001 ctgacgagtt gcaaaaagag ctgcaagagc gtttagcagt agcaggtgtc gaagtaatcg
     3061 aaacacgctt gaaccattta gcctatgcaa cagaaatcgc tagtgccatg ctccaacgtc
     3121 aacaagcaaa agccattttg tctgctcgac aaaccattgt tgaaggagct gtcacaatca
     3181 ctcaaatggc cttagagcaa atcgaagatg gacaagaaat caatttcacc gatgatcgga
     3241 aagtccaatt gatcaataat ttacttgtct caatcattac agacaaagga acgcaacctg
     3301 ttatcaatac aggcgacatg aaagaccgta agtgacatac tttttgtaag ataggaagaa
     3361 ggagtttagg acggattatt gtcttgaact ctttttttag ttgttttaaa gaaaatgaca
     3421 aaattgaaag aagaatgata gttgattcga aggcagaatt gttcgctttt ttctataatc
     3481 agtatataaa gtaagagaga aggggaaaaa gtgaaaaaac ttctgatcgg tctacttgtt
     3541 ttcatcgttg tggtacttgc aggggtgaaa atcgcagatt acgtagttat gggtggagaa
     3601 gaatactatg tacaaattac gacagatggt acaaaaacag tttcaaaatc agactctgga
     3661 caagaaacag tcaactatag atatatcctg cctggctata cgaaagaggg taaggaaaaa
     3721 gaactggact tcaagggaca attgactcga ccgcttcgta aaggagcata tttaaaaatc
     3781 acctggaaca aaaataaagg tgtcacttcg tatgaagaag tcgaaaaaaa agacgtgccg
     3841 aaaacagcac tggaaaaact aattaaaggg tagggaaaga aatgaacaaa atagtcgaag
     3901 tacagaatgt aacaaaagta tacggaaaaa acaatgagaa aaagacgcaa gcattaagcg
     3961 gcatcagctt cgatgtagaa aaaggcgaat ttattgggat catgggcgct tctggttctg
     4021 gtaagtcgac acttttgaat attttatcca cattagataa accaacagat gggcatatcc
     4081 gaatcaatca aaaagatgtg accacattaa aaggaaatca actagcagat tttcgtgcga
     4141 atgagattgg ctttattttc caagatttca atttattaga aagcttgaca gcacaagaaa
     4201 atatcgctgt tcctctttct ttacaaggcg tcagaccaaa agaaatcaag caacgtgtcc
     4261 aaaaaattgc agaacgttta tccatctctc atattttgga aagctatcct tctgagattt
     4321 ccggtggaca aaaacaacgg gtcgccgccg cacgtgcatt gatcacacaa ccaacgattc
     4381 tattagggga tgagcctaca ggtgcgctgg attccaaaag tgcccgggat cttttagata
     4441 cgatggacga attgaataca aaagatcatg tatcgattct tcttgtgacg catgatccgt
     4501 tttctgcaag ttattgccaa cgtattttat tcatcaagga tggcggtatc catcaagaag
     4561 tgaaacgtgg agatcaaagc cgtgattcct tctatcgaga aatactatct attcttggaa
     4621 acttggaaca gtagggggag attgactatg ctttggaaat tatcacttac tgggatcaag
     4681 agccgtttaa gagactactt ggtgttattc tcaggattag ttatggcatc tgctattttt
     4741 tacatgtttg aatcaatggc aagcaacgaa gcttttttga aaagtaatac gatcgttgcg
     4801 gctacagtta tcattttccg attcggttct gttttactag ggatcatcac attcgtttat
     4861 attttatatg caaattcctt tttgatgacg atgcggcaaa aagactatgc aatgtttatg
     4921 atgctgggag caaaaggcag aaaaatcgca cagatgattt tttctgaaac attcttagta
     4981 gggatcgcag ctactttagt gggttcagct gtcggtgtag gattgacaag tatcgtccac
     5041 cgattattag tggatcagtt agatctcaat atttcgcatt ttacaccttt tagtattcac
     5101 gggttgttga tcactgtttt gttttttgct gttttgtttt tattagctgc tgttgtcaat
     5161 gctttttcga ttgtcaaaaa accgatttta gcattgattc gtgcggatca gacaccaaca
     5221 agaatcaagc agcataaatt cttgtttttc cttgaagtag tactgggaat tgcttttcta
     5281 ggctgcggtt actatatgat gaaacacgtg gggacctatc aagtctttgg tataggggta
     5341 gccttagtta cgatcgtatt aggaacttat ttcattttcc attctgtgat cattttcttt
     5401 ttatcattac taaagaaatc agaacagatt tcattgaaaa aattgaacaa tttcacattg
     5461 tcacagttaa actttcgtat tcgtgaatat acacaaatgc tctcaatggt cgctatgctg
     5521 tttgccttgg cactgggcgc tttgacggta ggattaggtt ttaaaaatga aattgcagac
     5581 ttgacagaaa acaattctgg atatgatcta gtgatgaata atcctcgggc agaagatcag
     5641 caaaaaatca aagaactttc accaacactt tctgcttctt atactcaaaa agaagatgcg
     5701 ggaacgattt actataatgc aagtgaattt gcggctcaac cattgatcat gataaaacat
     5761 gaagatggaa caccgccgaa gatcacgaaa gaaaaagttc ctgctaaaaa aatgaatgaa
     5821 ctgactgtac aagaggaact tcgaagctat gaattgccgg aacagcaaga aaaagaaatc
     5881 aaggtagtca gtcaatcaga atttgatcaa ctgaacttgc cacaatccac tttacagcta
     5941 gttcaggtaa aagacttcca agcaaacctg aaaccaatcg aaacacttgt tgctcaaaat
     6001 aaaatgaaca atccatcttt gcaaaaagta gagtacagca accagaaata tgaaatgtat
     6061 gaagtgtata atggtctatt ctctggtttt gaatttatgg gattcttcct aggtttagct
     6121 ttcttgacca tgcttgctag ctgtttgatg ttcaagatct tgtcaggaag caagagtgac
     6181 attgctcgtt atgaaatgct tgaaaagata ggaacacgac gcgggttgct taaacaatcg
     6241 atccgtagag aagtaggcgt gttattctta gcaccaggaa tcttaggtgt agtccatgtc
     6301 ttattcggat tacaaatgtt tggtctattc atgcaaaatc catacaaaga tatttgggtt
     6361 ccgtttacga tattcttcgt attgtatttc atctactatg tactgacgac atggttatat
     6421 actggaatcg tattgaaaaa gagattgtga aaaagctgtc ctgcatcaag taataagaac
     6481 agccaagcaa aaatagctcc tcatattttt cgtttggttg ggcttattac cgcagatgca
     6541 agcttttgaa caccgtttgt tcggaaaaag aggttgtgac aatatttttg tcacaacctt
     6601 tttttcacgg tcttttatta gaatatagta aattttttct cgttactcac ggaataagct
     6661 gttaagaaaa cggtttccat gtatactgga atcagtgcaa atgaaagaaa agagggtgca
     6721 aggaacatgg aaatcaatga acaaaatttt ggcgaaaact atcacttgat tacgatcacg
     6781 aatcaaaatg ggacatcttt gtctgtcagt gatcttgggg cacgggttgt tcattttcaa
     6841 acaataatcg ataatgaaaa acgagagttg atcttaggtt ttgatgaagc aaaagaatat
     6901 ctagaaaaag acccttatat cggtgcttca atcggacgta tggctggacg gattacgaat
     6961 ggcacattca aattagatga tgaaacgttc gaagtaaaaa cagacccgga cactggtcat
     7021 tgtttacatg gtggtgcacc tagctttgaa gccaagcagt ggcattatac agtcatcaat
     7081 ggtgaaaacg aagcatcggt cattttctat cagactagcc cagatatgga aaacggattt
     7141 cctggaactt tagatgtaga agtacggtat acgctgacga acgatgatac atggcgagtg
     7201 acggtccaag ggatcagtga ccgcaaaaca gtattcaatc ctacaaatca tgtctatttc
     7261 aatttaaccg gtgatccagc acagtcaatc gacgaacata tactttggtt gaatagccga
     7321 ttatttgcta cgctcaataa agatacaacg cctacaggag aagtagtttc agtaagtggc
     7381 acagcatttg acttccaacg tcctaaaaaa ttaggtgagg tctttgccag tgattttcgc
     7441 caatcaatga tagtgggcgg catcgatcat ccatttttgt tgaaaaaaca atctggttta
     7501 ctaaatgctg cggaattgac aagcccagat gaaaaagtat cgatccaagt aaaaacagat
     7561 gcttcaggaa tcgtgatttt tacagcaaat tttggagaag atggaccgga aatgcgcggg
     7621 aataaattag taaatcatgg cggtatcaca tttgagactc aagaactgcc aggagcggag
     7681 cgacttgaaa gttttggaga tgtcactctg gaacctaacc aagtacgcga gtttgtaacg
     7741 gaatacaaaa taaatataaa gaaatagaat aaattaaa
//
LOCUS       DAYYHX010000128         7766 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000128 DAYYHX010000000
VERSION     DAYYHX010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.129"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..571)
                     /locus_tag="ACHA6Y_002029"
     CDS             complement(65..571)
                     /locus_tag="ACHA6Y_002029"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8988112.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            731..1171
                     /locus_tag="ACHA6Y_002030"
     CDS             731..1171
                     /locus_tag="ACHA6Y_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8988113.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(1197..2225)
                     /locus_tag="ACHA6Y_002031"
     CDS             complement(1197..2225)
                     /locus_tag="ACHA6Y_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988114.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(2280..2354)
                     /locus_tag="ACHA6Y_002032"
                     /pseudo
     CDS             complement(2280..2354)
                     /locus_tag="ACHA6Y_002032"
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2357..2713)
                     /locus_tag="ACHA6Y_002033"
     CDS             complement(2357..2713)
                     /locus_tag="ACHA6Y_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988115.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(3011..3985)
                     /gene="sgrA"
                     /locus_tag="ACHA6Y_002034"
     CDS             complement(3011..3985)
                     /gene="sgrA"
                     /locus_tag="ACHA6Y_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM8988116.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(4181..5020)
                     /locus_tag="ACHA6Y_002035"
     CDS             complement(4181..5020)
                     /locus_tag="ACHA6Y_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8988117.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            5205..6092
                     /locus_tag="ACHA6Y_002036"
     CDS             5205..6092
                     /locus_tag="ACHA6Y_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM8988118.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(6686..7380)
                     /locus_tag="ACHA6Y_002037"
                     /pseudo
     CDS             complement(6686..7380)
                     /locus_tag="ACHA6Y_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(7364..7762)
                     /locus_tag="ACHA6Y_002038"
     CDS             complement(7364..7762)
                     /locus_tag="ACHA6Y_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8988119.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2436 a         1532 c         1143 g         2655 t
ORIGIN      
        1 ttacgtaata aagcagaatg gataaaccag tatccattct gcttttttta aacgaagaaa
       61 caacttattg ctccaaaaat gtcagaaatc gtttatccca aattttctcg taaaaagcga
      121 ttgtttcctt tgctttcata aaaggattat cataagttgt cgttgctcct ctacacatca
      181 taagctggta tcctaatcca tgagcaaact tcacagtcgt atctatacaa tactctgctt
      241 gtgccccaca taattcgatc gaatgacagt ccaattcagt taataaaaca ctcaaattag
      301 tcttataaaa tgaattcgca tgggtttttc tcacaaaata actgtgctca ggaatagata
      361 attccgacaa tagctcccat ggttttgttt gtggcactaa ttgagcatct tcgtgttgaa
      421 cgaaaataac tggtttccca atcgtttcgt atgttttgat tcttttattc actaatgctg
      481 tcactgtttc taattcgtgt atcggcgaag aatcaaaaca tacccctttt tgtaaatcga
      541 tcactactat tgcgtcggct gtgtttgaca tacttgtgcc ttctttcttt tttcttgatt
      601 taatagtatt tcttatctag ctagttccat tatacgtgac aacattttct tattttacac
      661 ctctaaatgg cacttttcga ctatttcttc atgttattat ttcataaata cttagctatt
      721 ggaggaatgt atgatgatta aattgtatac aggttctggc tgctcgtctt caagacgcgc
      781 aaaacgttgg cttatcgtcc atgatatacc gtttatcgaa cagaacttaa ttcgtcaacc
      841 actaaccaaa aatgaattta tccacttact aaaattgaca aacaacggcg tgaccgaaat
      901 gctctctact cattctatta gttataaaaa tcttgttcac agaatagatg aactcagttt
      961 gagtgaacta tatattgaga ttcgtacatc cccttcttta ttgaaaaaac cgattatcta
     1021 cgaccaatct gcaaaacttt tgactggatt tagcgaagat aaaatcagga tgttcctttc
     1081 taaaactaaa agaaagcaag agctcaacga actcttgctt tctggtcatc ttgtcgattt
     1141 taaagaaaat aatctttttg caatgtctta attttggcat aaacaatctg cttttcttaa
     1201 ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat ttttatgatt
     1261 tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta aaatatctgt
     1321 gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac aatatcaggt
     1381 aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa tatccttttc
     1441 tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat gacaggttgt
     1501 tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa ccgtctgttc
     1561 ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa aaactgttgc
     1621 atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc tgattttcca
     1681 aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat cattgccaaa
     1741 ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt tttttcaaaa
     1801 ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc tggagtatcc
     1861 aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag gtaagcttgc
     1921 tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg ctctagtatg
     1981 taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat ctcttctggg
     2041 acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag taggctgatc
     2101 catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt ttctaatagt
     2161 tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa tgactgccaa
     2221 tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg attacctttt
     2281 ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct taattttctt
     2341 tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat tgataagtac
     2401 ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt atagaataaa
     2461 ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt tctgaaagaa
     2521 aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga atcaccgaat
     2581 tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca tataatttct
     2641 cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg atatcaataa
     2701 attcttcaaa caaagtacat actccttttt atctattatt tagacttatt ccgacagcta
     2761 catgaataac gtacaaatag attcgttact gatttttcag agatactacc atgcgcagtg
     2821 tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt tcctgtttct
     2881 caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca ggcaatacag
     2941 cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt agatctgttg
     3001 cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct cctacaatca
     3061 aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta ccagtttctg
     3121 gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca attccacgag
     3181 aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt ctcttttgtt
     3241 ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct gtgctgcttt
     3301 cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta ctttcactac
     3361 tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca ctactcgtac
     3421 tctccgtact gctttcgctg ctcgtgtttt ctgtgctaat ttcactgctc gtactctctg
     3481 tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta tccgtactgc
     3541 ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc gtcatctgtc
     3601 catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca atcgatagtt
     3661 ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct tttaaataaa
     3721 cttccccaat aactttttct gaacttccat taccatccca accatatgtt agaacagcat
     3781 gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg acttgttgtg
     3841 tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca tttgcccgtt
     3901 catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca agaataacaa
     3961 ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc ttctatttga
     4021 taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg aaaatctaac
     4081 aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa tgacagactt
     4141 aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa acagcagtgt
     4201 atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc aagactaaca
     4261 agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta attattgtat
     4321 tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg actggtatac
     4381 ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc gtaaataaac
     4441 ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata ttgaaaactg
     4501 ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca gcagatccaa
     4561 tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca cggaataaaa
     4621 gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc ggcacaatgt
     4681 ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta gtaataagca
     4741 gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct tttcctcgaa
     4801 cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga aataaagcta
     4861 gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat tcactatttc
     4921 caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa ccaacaaaga
     4981 ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc tcctttatat
     5041 taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt tctccatcat
     5101 agagagaata gatttttacg aactaagaat atctacagca accttacgaa aatgaaagac
     5161 tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga aattttttga
     5221 aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc tcaggttgta
     5281 gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt caggattttt
     5341 atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg attgtcttca
     5401 aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca aaagaatata
     5461 atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca gttggccaaa
     5521 cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac ggtttgaaag
     5581 ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc tatccagaag
     5641 ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca aaaaaagaag
     5701 caaatgaagg agaaaatttc tccaagcttg ttcaagctta tggaaaaaat aaaacattaa
     5761 aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct actgaagtaa
     5821 aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct gtaacagatc
     5881 caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag gataaaggtt
     5941 ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca aaaactcttg
     6001 atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat gtaactatta
     6061 aagatcctta tgtaaaaaat atattcaaat aaacaaattt gacccaattg gggatgtggc
     6121 aaaatttgag aagtgatttt tggaaattcc ttcttatttt tgaggcaaac cacttttgcc
     6181 acaacttcat cggggccttt ttttatttgt accaaaacaa atcactggat ttgttacaat
     6241 catgaggata taatttaaaa aagtttttta tttctatcac atgaatcaat gcgccattaa
     6301 gtagattctt tcatgcttcc ctattttttc acttgccgta tcgcttcaaa aacaactatc
     6361 ataaactcaa actcgactga aaagttattt taaaaaagac taaaaatatc ccttctggac
     6421 ttgttcttct tattctgtta tactagagaa acacgaaaga tatttttatg cgacactttt
     6481 catttgtttt tatgaaattt attcaaaact ggcgtaactt cagagttaca aggatgtatt
     6541 agaggttagg gaatacatcg tttaaatgag aaataagtaa tagttagtaa tagtcaccca
     6601 aactttaaat taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta
     6661 tcgtttattt tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa
     6721 tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa
     6781 tcgagggcat ctctaatact tgatctagta gctctttacc taacgttatc gctgagctcc
     6841 agatcgaatg gctgtagtga cttgttcaca atttttgaaa ctagctgcta atatttttgt
     6901 tggtctttgc gtacgttcga tctcagtccg caacgaatca atcactttca tagcatcaat
     6961 gccttgattt tgcatcctgt tataataagg tgcaatataa tctgctccat tattaatagc
     7021 taaataccct tgtaattctg tgtaaatagc tgtaccggta atcaaataac cttctttttt
     7081 caactttttg atcgcttgaa ggcctgcttg attcaccggc accttgataa acgtatcttt
     7141 tcccagttct tctatgataa ctcgagcatc ctctatcatt tcttcttccg ttcttcctat
     7201 tgtctgaata tggataggaa tctgtccgat tatttttttg atttctttca tatgcgagaa
     7261 gaagtttgac gctccttctt ttttcagaat tgtaggattt gttgttacgc ctgttaaatt
     7321 caaaaactgt tgacaagaat aaatactttt tgtatttgct gtatcaagca tgatttccat
     7381 atagctttcc cccattctga tcctcttgat cgattttaat gattgtcccc tttcttaaat
     7441 gcggcatttc cttatcttct acatacattg ttcctggaag ttccggctca tgagatccat
     7501 caaattttat cgtgatatgt cctaattcat tcaaattagt ttggacaaca tctcctactg
     7561 ctgtgattac atacatttgt ttatcaaaac agagcttcat atcacaagtt atttcttttc
     7621 ttacctgatt caattcaatc ttataacaat aatctgttaa tgtttccggt gcttcatctc
     7681 caaataaaat cattattttt tctttcctga atacttctgc ttctatacca acatctgtta
     7741 caaccgtttc aaagatcgtc atctct
//
LOCUS       DAYYHX010000129         7762 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000129 DAYYHX010000000
VERSION     DAYYHX010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.130"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(70..1491)
                     /gene="tyrP"
                     /locus_tag="ACHA6Y_002039"
     CDS             complement(70..1491)
                     /gene="tyrP"
                     /locus_tag="ACHA6Y_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM8988120.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1734..3611)
                     /gene="tdc"
                     /locus_tag="ACHA6Y_002040"
     CDS             complement(1734..3611)
                     /gene="tdc"
                     /locus_tag="ACHA6Y_002040"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8988121.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3902..5158)
                     /gene="tyrS"
                     /locus_tag="ACHA6Y_002041"
     CDS             complement(3902..5158)
                     /gene="tyrS"
                     /locus_tag="ACHA6Y_002041"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM8988122.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5270..5475)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5714..7038
                     /locus_tag="ACHA6Y_002042"
                     /pseudo
     CDS             5714..7038
                     /locus_tag="ACHA6Y_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376531.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(7107..7706)
                     /locus_tag="ACHA6Y_002043"
     CDS             complement(7107..7706)
                     /locus_tag="ACHA6Y_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8988123.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2301 a         1590 c         1360 g         2511 t
ORIGIN      
        1 aaaaactatt cacttggcag gacctcctac taaatcagcg acaaaacgtc ggctgaaaga
       61 cttatttttt tatttttctt ctaaactttt ttctacctca atcccccaag aatctttctt
      121 gaagtgattg atgatcaatg gaataacgaa catgaccact gtgatagctg ctactaataa
      181 tgcatcactc aatgtcgctg tacctaatgt agcaaatacg acaacgatga tagagaagat
      241 cagcatgcag ctgactaccc aagctaatcc gtttccgttt ttaccaattc ggtaaggacg
      301 ttcgaattct ggatgtttct tacgcatttt aagcaatgaa acagcaatca aaatgtacac
      361 gatacaataa ataacagttg tagcatttgt aagtgtcaag aatacagcac ttacgtcatc
      421 cattacacca taaagcaaag caaataatga aataacgatt gattgtgtta gaacgacatt
      481 tctagatacg ccgtatttgt tttctttatg gaatccgaat tttggtggaa ggaatccttc
      541 acgagctact tggatgattg tttttgatgg tccagtaacc caagctgata attgtaaaag
      601 cacaccaata aataccatga agctgaagat atttccgatc acttctggta agcctaggat
      661 ttgacagtca atcaagatag gctgcgtgat atttgctaat tccatttttc catctggtac
      721 gacatttgct acaagcatcg cattgatgac attcaataaa actaatccga tcaaagcgat
      781 aaatacgcct tttgtataat tttttgttgc gtctttcaaa cgaggcatgt aaacagaact
      841 catttcgatc ccaacaaaaa taaatgtgat accagctaag tatttcaatg aatcaatatg
      901 ttccaagtca gggaaagctt ttgaccatga aaagtcacct agatagccgt ttgagactag
      961 tccgactttg aaggctgcca aaacacctaa aacgaacatg atgactactg ggatatatac
     1021 ccctagccat acaccgatgt tcccgccgac ttttgccata tcgaatttca agttcaagat
     1081 cgtgatgatc cagtagataa ctaaaataca acctaaaacg aaccagtgat tattgccaag
     1141 ttctacatta ccaaatgtgt ttcctaacaa tggtcctaaa gtagaagcga ccattaccat
     1201 acctgggaac atttgcaccc ataatagcca tgcaacgaca aatccccatt tgctgcccag
     1261 tgctgttttt acccataact gaggaccgcc ttcttgagga agcatcgtgg aaagctcacc
     1321 ggaaaccatt gatatcggac cggcaaaaaa caaaactgca aaaattgaat aaaaaatcaa
     1381 tgtccaaccg actgcggcta acgtaggaat actccgaacc gttgcacaca gtgccattgt
     1441 cagaccgata aaggtcccaa gacccatttt acttacttca gatttactca ccgttgacac
     1501 tctccttgat tttttggata aaataacggt gtctttgaag tagtttcagc aataaacgaa
     1561 acaattcagc gaatatttga ggggattctt tgagtttatt gcttgaaggg tttcttcaaa
     1621 gacaacctca aaacaaaggg atcgaagagc ttaggtctcc ccttgctctt cagatccaaa
     1681 catgcgtcag aaacagttag tattcaagct gttttctaac atgattacct tgcttatttt
     1741 gcttcgcttg ccaataattg gtctgcgtag attttttcca atttttcttg aagagctgct
     1801 ttgatttttt cagcatattc ttcaaagtgt tcttctttgt tcatgtatgg tgtcattacg
     1861 ctggcacgca atacagttac ttttccggca cggttccatt cttcatctga gaatcctagt
     1921 tggttcacaa attgtaatgg gctgttgcca tagtctggga tagcaaagtc agtatgtgaa
     1981 gttaagaact cattaccgta aatgcttcct ttcacgtatg aagaataatc atatacgtca
     2041 tggttcaatt tattcattgc aactaagtca tcgttgcctt tttctttgaa tacatagtct
     2101 accatattga aatctggata agttaatgga tgaacttcga tttctttgtc tcctacttta
     2161 aatgacaagt cattcaagaa gttgtagaaa cgatgtgcac cttcgattga tgcacccatt
     2221 aatttaccat atcctgtgac attcaaaggt aagacatggt gtgctgccca aacgctggct
     2281 gctgttgccc ctgcttttga accttccaag atataagccc caagtaatgc tgggatatct
     2341 gcgccttttt caaatacata tgtcgcaaag taagagataa cgtctctcat gcggatatct
     2401 ttgatgacga taccaccggc agaatatgga acatagccca ttttatgtgg gtcaatcgtt
     2461 acagattctg cttcttcgat tgctttatat gcactgtgta cttcttctaa gatataatct
     2521 ttgttttctg taaagacatt gtatttgtaa tgaacatctt ttaaatcttc aaatggaatg
     2581 aagttgttgt cttcgtctaa gaaaatcgca cggccgtatc cgccataagc agcatctacg
     2641 tgtaagtaga agtaaatacc gtctttttct aatacgcgac gcaatgcaac gattttatcg
     2701 ataccgtcga ttgctccttc ttctgttgat cctacaacac cgactacacc taggattggt
     2761 gttttttcag ctgctagacc acgaacgatt ttttcaagtt cgttgatgtc catgcgatag
     2821 ttgtggtcaa ctggcactgg aatcacttgg tctaaaccga taccgatgat gtcggcagct
     2881 tttagccatg aatagtgttt agtttgtgga accaaccatt ttcctaattt ttctaaatgt
     2941 tttccgctac gtgctgagtg agctttgatt tcgtcgattt tttctgggac gctgtctaac
     3001 agattcatga tttcttctgt tgacaagttc attaattccc aatcgctttt tccagcaact
     3061 agttcaggtg tcacttcttt cattgcaagc ggcaatgatt tgatgttgcg ggcataccaa
     3121 agtccttcta agtttgctaa agaaccatca gcaacgatgt ggccccagcc atctttatag
     3181 ctcattaatt ttgcaaattc cattcctact tcttcttcca tttggcttgt tgcaggagaa
     3241 gattcatagg caacgttatt accattccat agcatagcaa aattgtacgc tagtaaagat
     3301 ggcatcaatg tttcagagtt catatgaccc cagtaacgtc cggcattatg ccaaggaacg
     3361 gaatgggtac gcatccgcgc tgaaatttct gatagaacat cctttgtttt attcacagtg
     3421 tgttcaaaac tagcactgct tttttcttct ggcgtaataa taggcatgtc ttgaggcatg
     3481 tagttttgac gccagcctaa atgttcatcc accaattcat tcaacaaagc tttatagatt
     3541 tggccgttct cagctttgtc cccaatgaat agggcattta ggtttagatc tttcgacaat
     3601 gattcactca tagtgaaacc ctcctaaaaa aatatttaaa ttttgtcgag ccctacatta
     3661 cacctgttct tctattctgt aaactaagtt ttggcctgat ttttaatcat attttaatat
     3721 gtatcaatta tccgaattgt tcacactttt gaaataatta aaaaaaacga gggaatttac
     3781 aaatgtaaac tccctcgcaa agattgtatt aacgacaacc agcgttctat tttaggatgg
     3841 gtttcaaaat tggaatgttg taatagtatt tccctttttc aaggtaaaaa cttactaaat
     3901 tctaatcaat tactttggcc aaaaagtaat ttttcttacc ttttcgaata accacaaact
     3961 taccatcgaa ttcatcggat ggatttatga caaaatttaa atcggtaacg cggtcaccgt
     4021 taatacttat agctccgttt gaaacatctt cacgtgcttg acgtttagat ggttcaattt
     4081 ttgtggaaac aagcagttcc acgatatttt caggtgtgct tgaaatttca acgttcggca
     4141 ttttaccgaa tccttgcgcg atttcttccg catttagttc tttaatattt ccagagaaca
     4201 atgcttgcgt tattttttgc gcttctttca agtcttcttc actatgcaca aatcttgtaa
     4261 cttcttctgc caaacggcgt tgtgcttcac gtttctcagg ttctgtctct acttttttcg
     4321 ctaagtcttc gatttcttct tgagacaaga aagtaaagaa tttcaagtat ttcactacat
     4381 cgcgatcatc ttgattcaac cagaattggt agaactcaaa tggtgaagtt ttcttcggat
     4441 caagccaaac ggcaccgcct gcagtttttc cgaatttcgt tccgtcagcc ttcagcatca
     4501 atgggatcgt taaaccgaac gcttctgctt ctggtccttc taattttcgg attaggtcta
     4561 atccagctgt gatatttccc cattggtctg ctccaccgat ttgcaattgc acatcgtatg
     4621 ttttatgcag atgcaaaaag tcgatagatt gcaggatttg gtaagtaaac tctgtaaatg
     4681 agattcctac ttccaaacgg ctggcaacga tatcttttgc taacatcgtg ttgatgttga
     4741 agtttttacc gtagtctctc aaaaattcaa gtaaagagat ttttgacaac cagtcgtagt
     4801 tgttcacaaa tgtgatattt gcatctttac caaacaattt tctcatttga ttggaaagtg
     4861 cgtccacgtt atgttgcact tgttccatcg tttgcaacac acgttctgtt ttgcggccgc
     4921 ttgggtcgcc gatcgttcct gttccgccgc caatcagaat atatggatga tggcctgcca
     4981 attggaatct tttcatcatc ataaatggaa tcaaatgtcc gatatgcatc gaatcaccag
     5041 ttggatcaac gccgcaataa agtgaaatgc tcttttcttc aacgagctcg cgaagtcttt
     5101 cttcgtttgt ttgctgatta atggcgtcgc gccaagttaa ttcatcaata atattcattt
     5161 tactgacctc cgtgtagttt ttttatgaaa gcaggaacct gctagcatgt gtaattaatt
     5221 atttcttcag gataggatag gaaataaaaa aagtcccttg gcatataagc caagggacga
     5281 aatctcgcgg taccacccaa tttgtatgtc tagatatcca acatacctct cgaacattta
     5341 acgctgtcgc gtggcaatca ctgccaaaga ctcctgaaat gtaattcgct ttaaagcatg
     5401 tactggtttg cagcgaccac cagctttctg aaacattggc tttaacaact acttcgtttc
     5461 gatcttcgtc ttgttattat taatatgtgt ctatcataga gcgcaacttt ttctttgtca
     5521 acagaaaaag gatatttttt ttaaattcct cattttttct tttttgaatc cgctctaatt
     5581 ccgaacattt tattttcaat tattgttaaa agaaataact ttttctgtat tttttctgca
     5641 atttatcgag aagtcacttt actttgcctc ttattctttc ttcgttacaa taaggaatgg
     5701 gacaagtaaa aaaatgaaaa agcaagggac gaggtatatg gaaaaaaagc aaaaaattat
     5761 tttaggagcc acttctgctt taactatcgt tattttaatt ctaagtttgt tttttgtcat
     5821 ccgatcatct acaaaaaaag aaccaatcat tcctgaaaaa aaataacaga aaaagcaaaa
     5881 gaaccgacag atgataagca acaactagtt actcaagcaa aaaatctggc tcaaagctac
     5941 gattatgacc aagcgattgc gttattggaa aaagatagta agaacggatc tactgaaaca
     6001 caacagcttc ttcaaacact aaaaaaggag aaagaacaac tgatcgactg gtctgatcct
     6061 actcagatct ctcatgtctt tttccacagc ttgatcgtgg acccgcaaaa agccttccac
     6121 tctcaacagg cacaaggata caaagattat atggtgactg ttgaagaatt caaccgttcg
     6181 atcgaacaac tttatcaaaa tgattttgtt ttagttaatt tgaatgattt gatccaaaaa
     6241 gacgaacaag gaaatctttc ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg
     6301 gttctttcac aagatgatgt cagctattat gaatatatgg ataattccgg ttttcctagc
     6361 cgattggtcg tatcaaataa caatgaaatc aaaaatgtct ataaagagaa agataaagaa
     6421 gaaatcggtg actttgacat ggttcctctg atcgatgcct ttgtcaagaa acatcctgat
     6481 ttttcttatc gaggcgcaaa aggtaccttg gccctgacag gttacaacgg agttttaggt
     6541 tatcgaacat caaaatctca atatggagac aatgaaaaaa caaaaaaaga gattaaagaa
     6601 gctaaaaaag tggcggaaca gttgaagaaa gacggctgga gctttgcttc ccatacatgg
     6661 ggtcacttga atatgactca agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg
     6721 cagcaagaag ttgcacctat cctaggaaaa acaaatattc tgatttatcc atttggtgca
     6781 gacatcagcg actggcagcc ttattcagca gaaaatcaaa aatttgctta cttgaagagt
     6841 caaggtttcg atattttctg taatgtcgat gcttctacac cagcttgggg acaattaggt
     6901 acggatttct atcgaaatgc tcggatcaat atcgatggca ttcgttttca atctgattta
     6961 gacggaaaaa atcctattct tgatccattc atcaatgtcc atgatgtcta tgacaaagaa
     7021 gcacgcgaat ctgcttgatt ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt
     7081 atcgtcagtt tcggcttttt tatgctttat ttcttcttcg gtgcgattcc cttgatcagg
     7141 acttctgtcc attctgctgt atatggaagc agttttgcgt atatctccgg cttagaaatt
     7201 ccttctacca ttgcttgaaa atagaggatc aagaactcat ccgaatattt caaatccact
     7261 tttccttctt tccgccctcg atggaaaaga tcaagcatga tactgaaact ttcttgcgta
     7321 tacgcatcca tcgcgtcaga aaattctttt ttttcttttt ttgtatagta tgtcatcaaa
     7381 tcgatataaa attgtttgtt cactcgattg agataacgga ttttgttttc actcatggca
     7441 atcagtgttt cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa
     7501 tcattcataa atgcaaaaaa tacttcataa atcagccctt ccttgctgcc aaaatattta
     7561 aaaatcgtcg ctttacttac ctgagcgttc tcagctacct tatccattgt cactccagcg
     7621 gttcctatgt ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt ctttttctct
     7681 tgtgctctct tttcaaaacc gttcatttgt agagcctccc gtaattttaa acttttataa
     7741 tatatatagt tcaaatttat tc
//
LOCUS       DAYYHX010000130         7748 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000130 DAYYHX010000000
VERSION     DAYYHX010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.131"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..205)
                     /locus_tag="ACHA6Y_002044"
     CDS             complement(<1..205)
                     /locus_tag="ACHA6Y_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8988124.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPV"
     gene            437..1171
                     /locus_tag="ACHA6Y_002045"
     CDS             437..1171
                     /locus_tag="ACHA6Y_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8988125.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            1210..2352
                     /locus_tag="ACHA6Y_002046"
     CDS             1210..2352
                     /locus_tag="ACHA6Y_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8988126.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(2447..3838)
                     /gene="sufB"
                     /locus_tag="ACHA6Y_002047"
     CDS             complement(2447..3838)
                     /gene="sufB"
                     /locus_tag="ACHA6Y_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM8988127.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(3843..4313)
                     /gene="sufU"
                     /locus_tag="ACHA6Y_002048"
     CDS             complement(3843..4313)
                     /gene="sufU"
                     /locus_tag="ACHA6Y_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM8988128.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(4300..5535)
                     /locus_tag="ACHA6Y_002049"
     CDS             complement(4300..5535)
                     /locus_tag="ACHA6Y_002049"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM8988129.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(5532..6818)
                     /gene="sufD"
                     /locus_tag="ACHA6Y_002050"
     CDS             complement(5532..6818)
                     /gene="sufD"
                     /locus_tag="ACHA6Y_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM8988130.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(6831..7604)
                     /gene="sufC"
                     /locus_tag="ACHA6Y_002051"
     CDS             complement(6831..7604)
                     /gene="sufC"
                     /locus_tag="ACHA6Y_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM8988131.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
BASE COUNT         2241 a         1590 c         1468 g         2449 t
ORIGIN      
        1 taacaggtaa cccgtattga gaaacatatt ttctaatcgt tcctactgat acacctagat
       61 attcacttgc ctgctgatag tccatatact ctagagtgac tccagaaata atgtcttcta
      121 gcataggctt tagcactgta attatctctt tcacttgttg ttcaacttgt atagaaacaa
      181 tgctatcaat gatttgtaga gacatatttc cccctccttt cgtgattgct tgaacaaatt
      241 ttctaagaat tatcttcgta ttttttgctc aaaataaaag tattttgtta ttaaccgatt
      301 aatcaaacaa aaaatgatta ccagttcatc gacatacaca taatacctcg ataaaaaaga
      361 ggtgtcaata tctttgtaaa atttatttac aacacccctt tttcgaggta tactaatttt
      421 aggaggtgtg gtcaatatga taaaacgtcc accaataaat tatttagaac gaaaaaagat
      481 actcggaaca aaaataaaag ctataagaaa gagtaaaaaa ttaacacaac cagcatttgg
      541 tcttatgatt aacaacggtc aattaattga caaaaaaact atctacgaat gggagaaggg
      601 aacctattta ccaataccag agcgattatc ccgaattgct gatttgggaa atatgagtat
      661 agaggagtta gtttgtggca atgtagaaga atatattttg ggaattattc tttatagaga
      721 tagcattgta ctagatggta taacatttcc agataaaaac ctgttccagc atcttcgtca
      781 acagttccca ccagtacact ctaatttaga tacatggctg gatagatatt ccaaattaga
      841 accagagatg caagaattta ttgctaacaa gacttgtaat aaagttaaaa atgaaaaaat
      901 tagtcttttc aatatcttaa aaatagaaga attattcatc aatgctattg tagaagaatt
      961 tgacaataac attcttttcc tcactagcag tatagaagaa ctactagaaa gaatggtaga
     1021 tgaatggcta cctattcaat tgaaagacat gtcttatccc gaagaagctg tgagagaaat
     1081 tactgataat ataaacaagc tagaacaaac gatttcatca attgggaaaa aatacactaa
     1141 gaaaaaaatg aagggaggtg ataccatcta gtggtaagta ctccttgtaa actaagagga
     1201 gatgatttca tggcaaaaat atcaaagtac aaaaagaaaa acggtcaagt ggcgtggatg
     1261 tataaaggac acattgccac cgatccacga actggtgaaa aaataaatac tacgagaaga
     1321 ggttttagta gcaaacaaga agctcaaaaa gattatgacg agtacagaca tcaaatatta
     1381 tacggtgtta aaaagaaagc acctgatatg acatttgaag acctgtataa tgaatggata
     1441 actcatcagc gtaccagtgt taaagcttca acaatcgcaa tctcagtaag atacgcaaat
     1501 aatcaaatac ttccagcatt tggtaagctt aaactatcga atatctctgt cccatattgt
     1561 caaaaagtgg tagatgaatg gcacagtaaa tatgaaagtt acgattatat gagaaaacag
     1621 actgcacaaa ttttacgcta cggggttgca atgcaatata ttgacaataa tcctatggaa
     1681 aaaacacttc ttccacgaaa aaaagagtat gagaaaaatc gtaaatttta ctcaaaagag
     1741 gagcttaata atttactgga tgcttttaaa gattttggta acatgaaaca atacgccttc
     1801 ttcagattat tggcatacac aggtatgaga aagagtgaag tactcgcttt acagtggaaa
     1861 gacatagaca cgtttaataa agaactacat gtcaataaaa ctcttgctgt agatgaattt
     1921 ggtaaagtta taattcaatc tccgaaaaca agagcttcta gacgagttat ctctttagat
     1981 actgagactc tttctatcct caacaactgg aaattacagc aaaaagaaga gtatttaaaa
     2041 cttggataca atactagctc taaagagcaa catgttttta caacagttaa aaacactcta
     2101 tacattccaa acactgttaa tgattggtta agatatattt tgaaaaaata taacttgcct
     2161 cgtattacac ctcatgggtt tagacataca catgctagtt tattattaga agcaggagaa
     2221 tctgtcaagg tagttcaaca acgattaggg catgaaaata gtaaagtgac attagacatc
     2281 tatgcacata ttacaaataa tgcacctaag aaaacaggtc aagactttgc tgatatgatg
     2341 gctcatcagt aaaagagctg gtcaaaatct ggtcatttct agtatacagg caaaacaaaa
     2401 aagctagaac ccttatatat caagggttct agctcctata acaggtttac ccaacactgc
     2461 cttccatctc ataactgatc aatcgattga gttcgactgc atattccatt ggaagttctt
     2521 tggtgaatgg ttcaacaaag cccatgacga tcatttcagt tgcttcagat tcggtcaggc
     2581 ctcggctcat cagatagtag agttgttctt ctgagatttt tgagactttg gcttcgtgtt
     2641 ctaaagagac ttggctgttg tggatctcat tgaatgggat cgtatcggat ttcgaccatt
     2701 cgtccatgat gatcgtgtca cattcgatat gagagatcga acccgcactg tttttggcaa
     2761 aggttacttg tcctcgatag tttacttctc cgccgtcttt tgaaattgat ttagagacga
     2821 tagagctaga tgtatttggt gcattgtgga tcatttttgc acccgtatct tggatttggt
     2881 ttgctcctgc aaaggcgatg gagagcatgg tgcctcgtgc accttttcca tctaagtaaa
     2941 cacttgggta tttcatcgtt gtttttgcac ctaggtttcc gtcgatccat tcaacggttg
     3001 cgccttcgta tgcttttgct cgtttggtta ctaagttgta cacattgtct gaccagtttt
     3061 ggatcgttgt gtaacggcaa tacgcatctt ttcttgtaaa gatttctacg atggcggcat
     3121 gcaggctgtt acttgaataa gtaggtgccg tacaaccttc gacatagtgg atgcttgctc
     3181 cttcgtcaac aatgatcagc gtacgttcga attgccccgt gttttccgca ttgatccgga
     3241 agtacgtttg taatggaaca tcgactttga cgccttttgg cacgtaaata aaggtcccac
     3301 ctgaccatac ggctgaattc agcgctgcta atttgttgtc ggttggcggt acaagttttg
     3361 aaaagtactc tttgaataat tcagggtatt ctttcaaagc agagtcagtg tcagtaaaga
     3421 cgattcctaa cttttcgaat tcatctttca tattatggta cacgacttct gattcatatt
     3481 gtgcggaagc accggctaga taagctcgtt cagcttctgg tataccaatc ttttcaaatg
     3541 tatctttgat tttttctggt acgtcttccc agtcacgagc gggtttgtca cttggttttt
     3601 ggtaatattt gatggcatcg aaatcgatgt ctgaaaggtc ggggccccat gtttgcattg
     3661 gcattttgtt aaatgtttct agagatttca gacggaattc aagcatccat tctggttcgc
     3721 ctttgattct tgacatctca cggatcactt cttctgtcaa gccttctcct gtactgaaaa
     3781 ccggttcgac atcatcgtga aacccgaatt tatattcttc taattctggt acgcccatgt
     3841 tctcactcct cttccttgtg gtaatgatgg aaagctgcgg taccatcgtt ttccattgct
     3901 tttcctagtg ctttccaggc aagtgtcgca catttgatcc gagcgggaaa tttagctaca
     3961 ccaccaagca tagcagcatc gcctaattgg tcgggatctt tgacttcttt tccttggaca
     4021 agctgtagaa agtctacagt taaatgttct gcttcttcta ttgattttcc gatgacggcg
     4081 tctgtcatca tcgaggcact tgccgtactg atggagcagc cactgccgtc aaaggcgata
     4141 tttttgatga ttccgtcttt tacatcgact tgcaattcga tcacatctcc acatgtgggg
     4201 ttgttcaatt cgatcttgct tgtcgagtca gtcaagcttc cacgatgatg cggatgggaa
     4261 gaatgatcca agatcacttg acggtataaa ttgtctaatt tagatagtcc catgttggaa
     4321 aaactccttt gttgccatga ttgcttcaac taaacggtcg gcgtcttgcg ttgtgttgta
     4381 gaaataaaag ctcgcgcgag cagtcgctga tacttcaagg tacttcaata gcggttgcgc
     4441 acaatgatga cctgctctga cggctacccc ttccatgtct aaagctgtcg cgacatcatg
     4501 gggatgtaac ccatccaaat tgaatgcaac gactcctgtc cgaaaattcg ggtcttgtgg
     4561 accataaaca gtcaatccat caattgcttg taattttggc aacacataag cggctagttc
     4621 ttcttcatat cgatggatat tgtccatacc gagtttttgc aaataatcga ttgcatggcc
     4681 taatgcgata gcgccggcga tatttggtgt ccctgcttcg aatttccaag gtagttcttt
     4741 ccatgtactg tcgtataatt cgacaaaatc gatcatttcg ccaccaaatt caacaggttc
     4801 catttcttct aaccattttc gtttaccata taaaacacca atacctgttg gtccgcacat
     4861 tttgtgtccg ctgaaagcaa agaaatcaca gtctagtgct tggacatcaa tggccatgtg
     4921 cggggcagat tgagcaccat ctactacgat gaccccgccg ttttcatgga caagttcagc
     4981 taattcttga acaggattga agacacctaa gacattggat acatgagcaa tggagacgat
     5041 tttggttttg tccgtaatct ttttcgctgc atcctccata tccaggaagc cttcttctgt
     5101 tagttcgata tatttcaaga cagcacctgt tcgttttgcc aactgctgcc acgggataac
     5161 attagaatga tgttccatgt aagagagcac tatctcgtct cctgctttga tatagcggtc
     5221 gccgaaggtt ctggctaccc aattcaagct tgtcgtcgta ccacgagtaa acagcacttc
     5281 tgctgtttct tttgcatgga tgaacgcccg tactttttct cttgcttgtt cataatcatg
     5341 ggtcgcgcgt tctgctaacg tatggacacc gcgatgaaca ttggcgttat cgtgttcgta
     5401 atagtacgct aatgcctgga gtacttgttt tggcttttgg gtcgtcgctg cattatccag
     5461 ataaactaaa ggttcatcat tgacgtcttg aaataagata ggaaaatcat tgcgtagctt
     5521 tttagcatcg atcatccatt caacttccct tcgattactt cgactaattc tttccgaact
     5581 tgttctacag ggattgctgt taagacagaa ccgaggaatc ctcgaatcac taagcgctct
     5641 gcttcttctt tatgcaatcc tctactcatc agatagtaca tttcttctgg atcaactcgt
     5701 cctacactgg cagcatgtcc agcagttact tcgttttcat cgatcaaaag aataggattc
     5761 gcatcaccac gtgctttatc agaaagcatc aagacacgac tttcttgttg ggcatctgca
     5821 ccttttgccc ctttcaagat gtggccgata ccattaaagg tcaatgttcc tttttctcgg
     5881 atcactccgt gttgcaaaat atgcccgatc gtatgagggg ctttattggt tactcgtgta
     5941 tcgattcctt ggatttgtct gcccgagctg attgccacga ttttgacttc tgcgtgtgca
     6001 ccttctcctg ctaaatctga gtcaaagtca gcgatcacat gaccgtcatt catgacacca
     6061 attgcccagt cgacagaagc atctcgtaaa atgtgcccac ggcgattcat ataagaagta
     6121 atattttctc ctaattgatc tacggctgaa tatttgattt ttgaaccagc tttagcgatg
     6181 acttccacga tgatattacc tgatgatttt gctacttgtt ctttggttgt ctggaaacgt
     6241 tccagataag aaaattcact atgttcatct gccacgatca acacatgctt gaagaagtgc
     6301 tcatcggatg cgccatcttg aatgaataag gactcgagtg gttcatcgat cacgacattt
     6361 ttaggcacat acaggaaaat accgctgttc ataaaagcta catggagtgc agtcaattgg
     6421 tcttcgttca tgtttactgc ttttttcata taatattctt ggactagttc tggatattcg
     6481 atcattgcag tgaacaagtc ggtaaaaatg actccttttt ctgctaattc cacaggcagt
     6541 tgttcaaaaa tcgtccatga accttgttgg acaagcacag gattgtcttt cattgcatca
     6601 aatgccggaa tcgtgcctgt ttcactgatg gtttgtgttt ctttcacatc aaataacgac
     6661 cagcgatgga atttcacccg atcgatatgc ggcaatggca gttcatctgc tttcgctagt
     6721 gcgtcttgac ggaaggttct catccaatca ggttcgcctt tacttaaaga aaaatcattt
     6781 accgcgtcaa gataatcgag ccagtttttt tctttcattc ttgctccccc ttacgcttct
     6841 tcttctttat agtcgattcc taattcttta ctgataccag cgtagccttc tgcttccaga
     6901 cgtttagcta ggtcagcatt ccctgtcatg actactcggc cttccatcat gatgtgaacg
     6961 acatcaggtg tgatatagtt caataatcgt tgatagtggg tgatgatcaa tgcaccgaaa
     7021 ttgtctccgc gcatttcatt tactccttta gcaacgactt tcaaagcatc gatatccaga
     7081 cctgaatcga tttcgtctaa aattgcaaat gtaggttcta acatcaacaa ttgcaggatc
     7141 tcattccgtt tcttttctcc gccagaaaaa ccttcgttca gataacgttc agccatttct
     7201 tctggcatat ttagtaaagc cattttttca tctaactttt tcaaaaatgc cataacagaa
     7261 attttgtcgt tttcatcacg tttcgcattg atcgctgcac gcatgaattc cgcattcgtg
     7321 atacctggta tctcacttgg atattgcata gctaaaaata agccaagtcg cgcacgttca
     7381 tctacttcca agtctaaaac atttttcccg tcaaacaaaa tttcgccttc tgttacttca
     7441 tagtttggat tgcccatgat agcggcagat aaggtagatt ttcctgtgcc gttaggtccc
     7501 atgatggcat ggatttctcc ggttttcatt gttaggttta cgcctttaag gatttctttt
     7561 tcctcgatcg acacatgtag attttttatt tctaagacag acatgcatta tacccccaaa
     7621 ttctctttcg ttctcactca ttttagacta attgagaatg aagcgcaatg accttcctta
     7681 ctaaaatcaa atatctttta tttattatta aacaattttg atttctaaat tagaataggt
     7741 ataaaaaa
//
LOCUS       DAYYHX010000131         7720 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000131 DAYYHX010000000
VERSION     DAYYHX010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.132"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..312
                     /locus_tag="ACHA6Y_002052"
     CDS             <1..312
                     /locus_tag="ACHA6Y_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988132.1"
                     /translation="ILSDKINVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQ
                     VGLIDGKYRIPIYVIEDYIWKLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPL
                     K"
     gene            complement(393..962)
                     /gene="thiT"
                     /locus_tag="ACHA6Y_002053"
     CDS             complement(393..962)
                     /gene="thiT"
                     /locus_tag="ACHA6Y_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM8988133.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(1028..1130)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1136..2107)
                     /locus_tag="ACHA6Y_002054"
     CDS             complement(1136..2107)
                     /locus_tag="ACHA6Y_002054"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8988134.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2201..3574)
                     /gene="glmU"
                     /locus_tag="ACHA6Y_002055"
     CDS             complement(2201..3574)
                     /gene="glmU"
                     /locus_tag="ACHA6Y_002055"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM8988135.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(4077..4895)
                     /gene="purR"
                     /locus_tag="ACHA6Y_002056"
     CDS             complement(4077..4895)
                     /gene="purR"
                     /locus_tag="ACHA6Y_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM8988136.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(5062..5886)
                     /locus_tag="ACHA6Y_002057"
     CDS             complement(5062..5886)
                     /locus_tag="ACHA6Y_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8988137.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(5859..6539)
                     /locus_tag="ACHA6Y_002058"
     CDS             complement(5859..6539)
                     /locus_tag="ACHA6Y_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8988138.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(6562..7500)
                     /locus_tag="ACHA6Y_002059"
     CDS             complement(6562..7500)
                     /locus_tag="ACHA6Y_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8988139.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
BASE COUNT         2216 a         1678 c         1336 g         2490 t
ORIGIN      
        1 atcttgtcag ataagatcaa tgtaaaaaaa tcttttttca aaaaagaaac acctgttgaa
       61 aaaatcagtg tggaattatt gaaagaaatg cgtaatttga tgcaggaagg gatcattttt
      121 tcttttgatc aagttggttt gatcgatggt aaatatcgta tcccaatcta tgtgattgaa
      181 gattatattt ggaaattaaa aggcacccgg tatcgagaag aggaaatcat cgctacttta
      241 atctactctg tcaccgatcg acactggcaa ttggtagaag atgccgatct ttcttatctg
      301 ccattgaagt agatctattt cttatcaaaa aataaaaagg aaacaagaaa ctaggacatg
      361 attctgtctc aagtttcttg cttctttttt atttacagct cctgtcgttt atctggcaca
      421 aaaatcgaag gagtcgtttg ttggatccct aataaaacga tgatcgttac aatagctgta
      481 ctcaacccgc tggcaccatt cattacgaaa gaaaagagcc aaggattcat cccccatagt
      541 gcatacgctc cccagaaaac cactcctgca ataaaatgcc aaaaatatct ggaaagtgcg
      601 ccaatcaatg caccaaacac gatgcttttt ctaatttgtt tcgtattatc gtctacatag
      661 cctttttgga catttgaagc catgagccct gctgcgcctg caaaagtata ggcaatcggg
      721 tactcaatca acacttgaac gacacttaaa taataaactt gtcccaacgg aaaatgtagg
      781 atgccccaaa tgaaacctgc cagcatccct gcctttgtcc ctcttcttaa cgcaaaaaga
      841 gtcaatggaa tctgccctaa tgaaatagaa aaactgctgc cgatctggat cggaatcaac
      901 gacaacacca tcgctaacgc tgcaacaatc gttccttcta cccaaactct attttttccc
      961 ataaaaaaac ttcctccttt gaataaaaaa gtcacaaagg aggaagcagc tttcgaaaat
     1021 agtcaaacag ctgtttattc acaattccta cgctcgcata accgatacag gtacgaaggg
     1081 tttagaaata gattcaatct cagcccaatt ggactcccct ttgtgagttt atttattatt
     1141 tattttttgt ttcaaacaat ggactaactg gtttgttttc atggatacgt ttgattgcat
     1201 ctcccattag tccgccaaca cttacttcat cgattttgtc gattttacga tcttctggaa
     1261 gatagatcga gtctgtaaca actagacgct cgatagctga atcttctatt ctttgtaaag
     1321 cagggccgga taatactgga tgtgtacatg aagcatacac actagttgca ccagcttctt
     1381 tcaaagcatt ggcagccaat gtgatcgtac ctgctgtatc gatcatatca tcgatcagta
     1441 cacaagtttt gcctttgact tgtccaatga tattcattac ttctgcaaca tttgcttttg
     1501 gacggcgttt gtctatgatc gcaatcggag atttcaaaaa ttccgctaat ttacgtgccc
     1561 gtgtgacacc accatggtct ggtgaaacaa cgacaacatc atcgcctttg attccttttt
     1621 ctaagaaata attggcaata agtggcgcac ccatcaaatg atcgactgga atatcaaaga
     1681 aaccttggat ttgaactgca tgaaggtcca aagtcagcat tcttgttgca ccagcttttt
     1741 gaatcatgtt tgctactaat ttcgctgtga ttggttcccg tgcacgtgct ttacggtctt
     1801 gacgcgcata gccataataa ggcatgacta cgttgattgt ttttgcactt gctcgtttca
     1861 gtgcgtcaat catgatcagc aactccatca agttatcatt gactgggctg ctcgttgatt
     1921 gaataacata gacatgcgat ccacgaatac tttcttcaat gtttacttga atttctccat
     1981 cactaaattg agtaacagat gattttccta actcgacgcc gactgctgct gcaattttct
     2041 ctgctaatgg gcgattggaa tttaacgcaa aaatttttaa tcttgggtca aagtaatgtt
     2101 ttgacatggg gacctccact ttctttttca caactctaga attctaccta ataaatagta
     2161 gtcatatttt ttaaataaat caagtgattt tgcaaaagtc ttagtttaga taagggagtt
     2221 tttttgcata tccttcttta ttgacttgtc ttgctctcgc aattgccagc gcatattctg
     2281 gaatattgtc tgtaattgtt gacccggcag caatcgaact gtttttagct acctcgacag
     2341 gtccgatgat attcgtgcta gaaccaataa agctgtggtc gccgacagtc gtatgatgtt
     2401 tgttctttcc atcatagttg acgaagacaa caccacatcc gacattgatt tcttctccca
     2461 gcgtagcatc accaacataa gtcaagtgac cgactttagt tcctttgccg atttgagcat
     2521 ttttcacttc cacaaagttt ccgatatgga cgttttcgcc aatttcagct tttggtcgta
     2581 aatgtgcata aggtcccaca tctgcatgtt tcttgacatg gctgctttca atgactgagt
     2641 tttcaatgac gacgtgatcc tcaatcctac tgtctacgat ttttgaatgt gacccgatga
     2701 cacaatcaga accaatgact gtatttcctt ggatttgtac acctgcttcg atcaatgtgt
     2761 ctggaccgat ttctacgcct gcatcgatgt atgtcgttgc tgaatcgaca aagcttacgc
     2821 cattgaccat atgcatctgg ttaattcgct ttctcatgat ctcatttgct ttagctaacg
     2881 cgatccgatc attgactccc attgattctt caaaatcatc cgtttgataa gcagcaactg
     2941 tatggccttc ttcttttaag atttcgatga tatctgttag atagtattca ccttgagcat
     3001 tgtttgttcc aactttgctt aacgcatcga acaaagcttc gttgtcaaaa cagtaagttc
     3061 ctgtattgat ttcttgtaca agagcttctt ctggtgttgc atctttttgt tccacgattt
     3121 tttctacgat cccaatatgg tcacggatga ttcgtccata tcctgttggg ttttctgctt
     3181 gtgccgtcaa gatcgttgca cttgcatttt ttccttggtg atattcaaaa agattgttca
     3241 gcgtttcagt agtcaataat ggtgtatcgc cactgataac taacgttgtt cctttttttc
     3301 cttccaagaa agaagcagct tgcaaaacag catgtcctgt tcccaattgt tctgcttgta
     3361 acgcatattt actgcgttct cctagttgtg ctttaacttg ttctgctccg tgtccaacga
     3421 tcgtaatcac ttcatctggt ttcgtttcac tgacccgatt aatgatatgt tctaccatag
     3481 gtttaccaga aacgggatgt aaaactttat ataattttga cttcatgcgg gtacctttcc
     3541 ctgccgccaa aataattgca taacgtgcgt ccaactgcgt cactcctaaa tatagtttgc
     3601 tgatttcagt ttatcccagt atacccattt tttcaacaaa acaagtgatt ttcttttatc
     3661 ccagcgtttt ctggataaaa aaatagatag accataacgg gcctatctat ttggtaaccg
     3721 gtgctctcct gccaactcct cggtcacaag tcacactact cataaaacat gaaaagctgt
     3781 tttttgaaat cgtttcttgt gacttagagt taaacggtag gcttacaacc tcttcattaa
     3841 acgtttttca atcagctgac cttcggtatt agctcaataa acggcaaaat atgaggagca
     3901 atttttccgt ttattgagct aatactcagg acaggacgct gatttcacaa cctctagata
     3961 aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatgaaaaa atgtggaaag
     4021 ccattttcac aaaatttttc ttactactcg aaactaagca gctttttcac aacctcttat
     4081 tctgaaaaat aatttcctgg cacaaccgta atatttttcg tttgagtatc tacatctttg
     4141 acataaagca gtgacgtata atcatcgatg gcgcgatgac cgttgaattt tgattcggca
     4201 aatacagtga tgccaacaag ttctgattcg aattcttcaa tcaagctctt cattccgttg
     4261 actgttccgc cgcctttcat gaaatcatcc actacaagga cacgtgaacc acgtttcaaa
     4321 cttctcttag acagttccat cttttcaatt ctttcagaag atccagacac atagttgaca
     4381 cttactgttg atccttctgt tatttttgag tcccggcgta caataacgaa tggcgcattc
     4441 agataatagg acacagcttg ggcaataggt acgccttttg tggctacagt cattacagca
     4501 tcgatttttt caccaaggta tttagaagca atgattcttc caacttgacg cagcagttca
     4561 ggttctccta acaaatcaga gagataaaca tatcctcctg gaagcagacg gtcttgttct
     4621 gatagacgtt ctgctagaga ttcaatatat tttttcgcct cttcatatga aatttcaggg
     4681 atgaacaata caccacctgc agcacccggg attgtttcca aggttccata ccctcgttct
     4741 ttaaatgttt tcttgatgat tcccaaatct tcactgatag aagatttagc agattcataa
     4801 cgttcggcaa agcttgttaa ggaaatcaat tcatgcgggt gattcaataa atactgtgtc
     4861 atgtcaacta gacgttcact gcgacgtact ttcacctatt ccaactcctt ttaagaaatt
     4921 ttatcgtttt acattatata agtttttctt caaaaattcg agcattttat gaaaaaataa
     4981 gaaaatagtt cggtatttat taaaataaaa agaagcctat aaaaaatttt tcgtttttat
     5041 aaggcttctt ttctttataa tttagttatt ttttcttctg aaggcaacca atattcgttt
     5101 gatgatattt acaatcagga acaaagcaat aaagactaat gtgattgttg ctcctggagg
     5161 tgtatctaga taaaacgagc tagttagtcc tgacagcatc cctagaaatc ctacacaaac
     5221 actgatagca atcacgacat tgaagctttt gcctaatcgc ataccgattg ctgcgggaag
     5281 aaccatgatt gcggatatca aaagtgcacc ggcaatcggg atcatcacag caatagccac
     5341 accagtaatc acattgaaca tcatcgacat caaatgaacg ggtagcccat ccacgtgggc
     5401 ggtatcttca tcaaaagtca gaacatacat cggccgtttg aataaaaaga acaatatacc
     5461 gatggcaacg aataagattc ctaaaaagat gacttgctcc catgtaatgg tcacgatcga
     5521 accaaacaga taggattgga tacttgttgc tgaattccct ccgctcaaat tcatcaatac
     5581 taacgctagc gccaaaccac cagccattaa aatggcaatg gagatttctg aataggttcg
     5641 gtagatcgtt cgcagatact ccaatatcac agctgctaat acaacgacta gcatggtcgt
     5701 aagtgtagga ttaaggttca aaaagaatcc taacgctacc cctgctaaag agacatgaga
     5761 aagcgtatca gccatcagcg attgtcttct gatcactaaa aacacaccta gcatcggggc
     5821 gattccggca atgaagatag cggcaagaaa cgctcttctc atgaattcat atgaaagcaa
     5881 cgccattgag aatcctcctt tctcaccagt cggatctgtc tgtccgcata ctgtttgata
     5941 tcttcatgat catgggtgat cattagaact gatttcccat ggacatgcgc actatgtcgg
     6001 agaagacgat aaaattcatt tctggattct tcatccatac ctgttgttgg ttcatcaaga
     6061 acaaataagt cagggtcagt agcaaaaata cgcgctaaac tgattcgctg cttttgtccg
     6121 ccagacaatt cacctattcg ccgatttcgc atatcccaca tccctacagc ttctaaagca
     6181 cgctgtacat gttgatgatc tttttcagag agacgcttga accagcggcc tcttggatat
     6241 cttcctgaac ggactaattc gatcaccgta ctaggaaaac ctgcattgaa agatgcaact
     6301 tgttgaggaa tataaccgat acttaatttt tttccttctt gatttttctc agcaatcttt
     6361 atcattcctt ttgaaggttt cagtaatcct aacgatgctt tcaccaaggt tgacttggct
     6421 gctccatttt ctccagttaa aatgacaaat tcacccgcat ctacatgata agagacatct
     6481 tctagtacag gttcatcatc atagtaaaag gtcaaatcag atatttcgat ataacgcacc
     6541 ttacaacctc cattctaaaa cttaattgat actttctttt aaagctttca gatttttttc
     6601 catgacagaa atataatttt cgcctgcttt gatttgttca ttagtcaagc tttctaaagg
     6661 attcaacact tctagcttca ctcctgtttc attcgacaat gtctcagcta ccttcgaaga
     6721 ggcattttct tcaaaataaa tcactttgat gttgtttttc ttcacaaatt cttttagctc
     6781 agctaagcga cttggtgtgg gttcctgatc tggtgaaacg cccgaaatcg ctacttgttt
     6841 taagtcgtat tgattcgcta aataagcaaa agcagcatgc tgagtgacaa atgtgcggtt
     6901 tgttgcatct tttagctcat cggagtattt ttgatccagt gcttcaagct ttttgatata
     6961 agcatcggca ttcttcgtaa aagtttcctt tttatctgga tatttttcgc ctaattcttc
     7021 agcaatcgtt ctgacttctt ttatagctaa taccggatcg agccaaacat gtggatcgta
     7081 ttcatgtgaa tgaccatgcc cttcttctga ttcttcatgt tcgtctgctt cttgcgattc
     7141 taaacggtcg attcctttag ctgcttcgat catcaaggtc tgtttgctat ccactgaatc
     7201 ctttaacgaa tccacaaaaa attccatgtc tgaactatta tacacgaaaa catctgcatc
     7261 ggaaatttct gctctttctt ttgcacttgg ttcgaaatca tgcggctccg ttccagcagg
     7321 gatcaaaagt ttcacgtttc cttcatcccc tacgatctct tttgtaaaat catacatcgg
     7381 ataaaaagtc gtaacgatcg tcaaatcctc ttttttgtct gcttccttgt tcgtatttcc
     7441 acatgctgca aacagtaaag cacctatcat taccgtagct gtcaaaagat attttttcat
     7501 ttatatgctc ctttcaatga acaaatcgga attttacgat ttattaacgt gaactaatgt
     7561 atcaaaataa aaaaacacag tcaatagaaa tccatcaatt tctgatttaa gcatgtatat
     7621 tcttgattaa atcgtaaaat ggatgagttg ctgctcatat ctagccattt cactacaata
     7681 aaaaactgaa gaataccatt ttaggattcc tcagtttttc
//
LOCUS       DAYYHX010000132         7647 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000132 DAYYHX010000000
VERSION     DAYYHX010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.133"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..2344
                     /locus_tag="ACHA6Y_002060"
     CDS             17..2344
                     /locus_tag="ACHA6Y_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFM8988140.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2341..3255
                     /locus_tag="ACHA6Y_002061"
                     /pseudo
     CDS             2341..3255
                     /locus_tag="ACHA6Y_002061"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3328..3843
                     /locus_tag="ACHA6Y_002062"
     CDS             3328..3843
                     /locus_tag="ACHA6Y_002062"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFM8988141.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3840..4286
                     /locus_tag="ACHA6Y_002063"
     CDS             3840..4286
                     /locus_tag="ACHA6Y_002063"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8988142.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4436..5251
                     /locus_tag="ACHA6Y_002064"
     CDS             4436..5251
                     /locus_tag="ACHA6Y_002064"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8988143.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5457..6629
                     /locus_tag="ACHA6Y_002065"
     CDS             5457..6629
                     /locus_tag="ACHA6Y_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8988144.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6839..7285)
                     /locus_tag="ACHA6Y_002066"
     CDS             complement(6839..7285)
                     /locus_tag="ACHA6Y_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988145.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(7282..7476)
                     /locus_tag="ACHA6Y_002067"
     CDS             complement(7282..7476)
                     /locus_tag="ACHA6Y_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988146.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2574 a         1444 c         1525 g         2104 t
ORIGIN      
        1 tttattgaga ggagggatga aaatgattaa gaaaactcaa aataaaatcc aacgtatatg
       61 tcaaaaacat tttgtttcta gactcaaagc tgtatctaaa agaaaatact tacatcccat
      121 tatgatttcg atcatcttga tttttacttt cttagggagc ttatcaagta tttatgtaaa
      181 cgtttataca gaaacgtcaa attttgctaa ccaaacgaca gaggagacaa atagtccacc
      241 ctattctgaa atacagtcca tagaaagcac caatcagtct agcaacaacg agacaacctc
      301 aaccacatcc tcgactcaac tgattgatcc gacagaaaaa gaaatcacag cagcaaaagc
      361 agcaggggag aaagcttatt cagaaacagg tagaagccag acatttagta ctgttgcggc
      421 cactgcgaat ttcaacggac ataccatacc agatggatat acagtgatat atgccaaaga
      481 tggagtcatt gttgcggtta acggatcgat gagtgcggat aaaactgcgt tacaaggcgt
      541 aaccaaagca atcgctttta ttgataaccg tccagatatc cataatctgt ttattggctc
      601 cacagtaaat ggtactacta tcacattccc aaaaagtatc tatatggggg tggctaccgc
      661 tggaaatacg atcgattcag ttacacccaa tgcggataaa agttattctt ttacaccctc
      721 aacatgggga gggcttcctg ccggaggagg tatgagtact ttttcaatcg gtatacagtt
      781 agcaaatgat attccattca cggggtactc gatcaaaaat gggcagatcg ttgatggtaa
      841 gggaaatgtt tcaaattttc caacctatca agcagcaacg gttcaggtca atgcgaatcg
      901 gacaggaact ttttcttata cagtcaatgg tggagcagca aaaatcggaa cgtacgggac
      961 tacattatct ggaatccatg tgggtgacaa agtggttatc actccaacag cagtaacggg
     1021 ctattctttt gcacaaacaa acggttcttt cacagcccta gaaagtaacg tcgatacggt
     1081 gacctataca ggcaaacaaa caccaatcac tattaagtat caagactatt ttggacagat
     1141 gattgctcct tctaaaataa tgaatcttac ttacggaagt gctgcacaag acctgacgac
     1201 taatacgcct actatcaacg gatatacctt tacagctgtt tcagcgatag aaacaaagaa
     1261 ccaatcagca atgagtgttt tagctagttt agatacaaat ggaaatgtaa tcgtaaagga
     1321 cgcaaatgga aaacagattt ctgaagtcat cctatattat aagacaaatg caacagtatc
     1381 aatcaatgca aatggcagta agtactatga tggtctttct gttgtaccga tcgttaaata
     1441 tgcctttaat gataaaacag aatcaacatc ccttttaaac aatgatctca gtattgatca
     1501 aatcacatgg aatccaactg atttcaaagc gatgaatgaa aagggagttg aagtaactgc
     1561 gcctactgaa attggaacct atacgtcttg gcaattgaca aaaagcggac tagctaaact
     1621 agctgccaga acaaactatt tatttactat cgtgcagaca tctgatgtct atacgattaa
     1681 gcaaatcagc ggtgcggtag agctcagtgg cagtaagaca tatgatggaa aagcaggtgt
     1741 cccgtcaatc catgtcaagc tagcagaggg tgtcactagt tcgcttactc cagtacaagt
     1801 ggcacttagc agtatggatt acacagcaga tgcacaatca gcaaaaaata tggttaatgc
     1861 tggcagctat acaattaaac taacgaatag cggaatagac aaagtcaaag cagcaaatcc
     1921 caagttgtca tttactgata tagccaacac atcaggaact tataccatct ataaagctaa
     1981 cgcagtgatt actgtcgatg acgctgcctt taattatgat gcccaaagtc actcgatttc
     2041 ggttggaaat ctccacgtta ccggtgttgt cttaggcgaa tcattggatt ataccctaac
     2101 agttaacagc cggacagata ttggatcaca aatagtaacg attagtttag gcgaaggaag
     2161 tgtaaacaat aattatacga ttatcgcaac agataccgct aagttgacga tcaatccgac
     2221 tctcttgctt ccttcgacag gtgggatagg tctactgccc tttgtctttt taggactgat
     2281 ctttattttt tctggcttct tttattttat ccatcgtaag aaaaaagcag gtgaacagcg
     2341 atgaaaaaaa caaaaaatgt acgtgtggat atatttgctg ttctcttttt tatcctactg
     2401 gtctttttta taggttctcc gactgtgtca gctagagaat cacctattac atttactttg
     2461 cataatcgaa tgttcgaaag cgataagatg ccaagtatca gtcaaaacga cggaataaat
     2521 atcccggata actttcaagg agtaaaaggg ttgaacggcg ttactttcgc tatatatgat
     2581 gtctcggatg aattttacaa gctgcggtca gaaggaagta gtgtggaaga cgcacaaaga
     2641 aaactagcac aaaaaagtga tagtgaaaaa atactggcag aaaatgtgac aaaaacagta
     2701 gacgaggaag agggcattgc ttcttttagt gcatcggaca aagatgagca aggtcgtgac
     2761 gctgtttacc gatttacgga aataaagact tcagattaag taaaagaaaa atcagcacca
     2821 tttgtcgttg tgcttcctgt gacagattca tctaatcata aattgacgac catccacttg
     2881 tatccaaaga gcgaacaaaa aatacctgct gcactcacat taacaaaaac agtagagaat
     2941 aagcagacag attttgcaga tggggataaa gtcccttatc tcatcaccgt aacaattcct
     3001 gagaacatca acgagatagg aacctatacg atcaaagata cagctgatcc tcagttatgg
     3061 ctagaaccag aaacgatcga ttttttgatt ggtggagaca agattcatac atttcacaca
     3121 caaaaaataa aacatggttt tgtcttatca gattctggaa aagatcttag ttcgtttgct
     3181 ggaaaaaaat taacgatccg ttaccaaatg acattgaaag aaaacagtga aaaaacgaca
     3241 tttgataacg atgtacgttt gacgactgac aatcaaacag tagaaacaca tcttgccatc
     3301 caaacaggag gcaaacaatt tgtcaaagtg gatcggcaaa cgaaacaaaa actagcagat
     3361 gctcaatttc tagtcaaaaa aaatgaccaa tacttagcgc aagaaaacgg gataaatcat
     3421 tgggtagcca aagatgattc aagagcaacg atttttactt ccagtaaaga tggaacattt
     3481 tctgtccatg gattgtcttt tggaagttac gaactagtgg agattaaacc gcctaaagga
     3541 tatatattga gtcaatcaac cattctcttt gaagttgaaa aaggcagtta tgatccgctt
     3601 catcctatac cattggaaat catcaacgaa ccaaaagcaa atccgacgaa taatggaaaa
     3661 acgccagttc agatgcagac aaatggtcca tcaaatgaaa aaaatggagg aagctttcca
     3721 aaaacaagcg aagagatgct tggaggtttc tcaattttag ggttattgct agtcctttca
     3781 acagggacag cttggtttta taaaaaacaa caaaaaggaa acaataggag ggaaataaaa
     3841 tgaaaaacat caaaacaaaa atatggacgt ttttaggaac tgctatcatg ctgcttcctt
     3901 tcgttctcgg cctaggaact gcagaggttt ctgcagccgt atcacctaca ccagaaaatg
     3961 ttacggttaa tttgcataaa ttgaaattca cttcagcacc agaaaatcaa ataaacaacg
     4021 gaacggaatt gacatttccg aatagcgaac cgttgaacgg cgttgaattc aacgtatatg
     4081 acatcactgc tacgtactat ccaagtaaag acacagcagt tccagcagac gctacaccct
     4141 tcgcaagtgt aacgacttct ggtgaaggat tagctaacct gactcttcca ggtaaatctg
     4201 atgggaaaga tgctgtatac gtctttgtag aaacaccaaa gcccggtgtc gaaacgtcgc
     4261 caaatatcgt gttaagctta ccctagaccc tgacaaacct ttagacacga ttcacctata
     4321 tgctaaaaat gtcatcaaag aaagcaatat caaaatccag aaagctggga acttttcagg
     4381 ggtagataag ttagcagggg cgcaattcca gatcaaaaat tcagagaatc aattcgtgac
     4441 cggttttgat gaaaacactg gtgtcgctct ctatgatcct gctgagtcag cgttgacatt
     4501 cacaactgat actgaaggat ccatcagtgt caatggatta atagacggca attatacttt
     4561 agttgaaaca aaagcaccag atggttatca attaaccaat ccacaaaaag aaactacttt
     4621 tatagtaaaa aacggaaaat tgcaacaaac aaccggtatc atccatggcg cagatgaagc
     4681 caatcttggg aatccaaacg gtgtagtcaa tcgggaaaat acaactacgg ataacacgat
     4741 tactgtagaa aatgtccaaa actacggtga tttccacttc atcaaacaag atgtgaacac
     4801 gcaagaaaaa ttagccgatg cagaattttc tattctaaga gcaaaagatc caactgttaa
     4861 attaatgaaa aaaatagcaa accttgcctc aggagaatac ccatatgctt ggtcagatga
     4921 agtcacaggt tctgactggg aagaagtgaa attgatttct gattcagaag ggaaattttc
     4981 aatcgaaggg ctgaaacaag gggcatactg ccttcaagaa acaaaagcac cagaaggcta
     5041 cgcattacct aagaatgaag ctgcgtacac tgaatttaca gtagatgatg atccaacaac
     5101 ttctgatgaa acaacgatcg ataaccaacc taaaggaatc ttgccttcaa ctggtggtat
     5161 ggggatcatc gtattcatct tggtaggtac tgctttagtt ggaggagcag tgatctattt
     5221 caaaaaacgc catcaagaaa cagaagctta aactattgga aacaaaaatc atacgataaa
     5281 taaacgatag tgattcaaat ggggagaaag gttgtgacaa gtggtcggtc tcttggtatt
     5341 cgctcaacaa acggcaaaat aggaggaact attttgccgt ttgcttcgct aatactcagg
     5401 tcaaggcgct gatttcacaa cctcttcata tacatattag aaaataggtg gacatgatga
     5461 ataaaagcag aataagaata ctggacatct tcatggcaat cattttagtc gttgggatcg
     5521 ggatcttttc ttatcctttt gtagaagatt cactgaatga ttttcttgcc cagcaaatga
     5581 tcatccatta tcaaaagcaa gcatcgaata agaattcaga ggaaataaaa aaacagcagg
     5641 aaaaaatgac aaagaaaaac caacagttgg cagagcaaaa cgtatcacca gggataggca
     5701 gtttcaacca agcagttgat gcaaaagcat taaaagattt acctagtaat gcctttttta
     5761 tggaacatat gcttggagtc atcgaaatcc caaaaatcaa tgtcagctta cctatattcg
     5821 atcagacaac ggaaatattt ttgcaaaaag gaacgtcatt attggaagaa agttcttatc
     5881 cgactggcgg gaaaaacacc catgccgttc tttctgggca tcgtggactc ccagaagcaa
     5941 aattatttac agatttgcct aagttgaaaa aaggagacca gtttttcatc cagatcaatg
     6001 gaaagacatt ggcgtatcaa gtagaaaaaa tccaagtggt cttacctgat gaagtagatt
     6061 ctttgggtat ccaaaaaggc agagatctcg tgactttgct tacctgtact ccttatatgg
     6121 tcaatacaca tcgactgctt gtgacggggc atcgtattcc ttatcaagca aaagaggcaa
     6181 aaaaagcgat ccaaggaatt gatcaatgga aaaaatggaa attttttatt tggtttatcg
     6241 gtatactact tggatcaatc ggcttagttt ggctactcat cgcttattta gattctttgg
     6301 ccatcgctaa gcgaaactat cctttatctt tttatgtaaa gaacacaaac ggacgaccaa
     6361 tcgaaggaat ggtcttttct gtaaaaacgc tcaacggcaa acactatatt actagagaaa
     6421 aagttccatt tgtgaaagct tctgatgaat acggactagt gagattttct gatttaaaag
     6481 gcaggaacta tcgcttgcaa catgaggagc tcctgcttaa aatccatgta aaacataaac
     6541 attcgaaaca gttttcaatg aaattgaaaa aaggaagata taaactgcga aaagaaaaag
     6601 aggtgtacta tttgattgag aaagaatgaa tgaagatgtc caaaaaagca gagggaacag
     6661 actaaatgaa aaaagagaga agctgacgaa atggacgtgt aaactgttat ttttggttta
     6721 ctatctggaa cgccatcttc tattttttta tgtttcagaa gaattttatt attatagtat
     6781 aacttttgaa ttagtttcca taacatttgg agcaaggggt taaaccacga gcttgagctt
     6841 agcttaaagt ggcaggggta tacgtaccat ttccacattt atgtgtatgg tattttgaac
     6901 cggtcggtgt gacatagaca gtttgttcat tattttgctc aggagctgcg gcttgtgcct
     6961 gctgagaggc ctgttcagct gctgattgct gtcgcgcctg ttctgcttgt tgctgtgcga
     7021 ttgcttcttg cctcgcctgt tctgcttgtt gctgtgcgat tgcttcttgt tgcgcttgtt
     7081 ctgcttgttg tcgtgcaatt tcttcttgct ttttccgttc ttcttctgct tcttttgctt
     7141 tgattgtgga atcaattgca gcaagacggc ttgtcaactc ttgtttgttt tcaggtagcg
     7201 cttggatagc tgctgaagca gttgcatagt tttcttttgt aggatttgct tcagcttgtg
     7261 acaaagcaga ttccgcttct gtcacttttt ttctagtgca gctttttccg ctttttgttt
     7321 ctcttctttt ttcttttcaa tttgttcttg ttctttcgat gaatctgccg tactttcgga
     7381 gatcgtggta ctgctgctgg aagcctgatc tgaacttgtg ctttgagtag tagacgtagt
     7441 agcaccacac ccactcaaca aacttcctgc gaccaaaaac aatgcaacta attttttcgc
     7501 taaatttcct ccttaagtaa aaaacaaccg ttttcaataa atgtcctttt caattgttta
     7561 cttacttgtt ataataattg tgttatgaat agtgattgag gaaacttaat ctaatcccgt
     7621 gttgatacac gggatttttt gctatag
//
LOCUS       DAYYHX010000133         7460 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000133 DAYYHX010000000
VERSION     DAYYHX010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.134"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..288)
                     /locus_tag="ACHA6Y_002068"
     CDS             complement(<1..288)
                     /locus_tag="ACHA6Y_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988147.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(406..666)
                     /locus_tag="ACHA6Y_002069"
     CDS             complement(406..666)
                     /locus_tag="ACHA6Y_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988148.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(803..1216)
                     /locus_tag="ACHA6Y_002070"
     CDS             complement(803..1216)
                     /locus_tag="ACHA6Y_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM8988149.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1672..2157)
                     /locus_tag="ACHA6Y_002071"
     CDS             complement(1672..2157)
                     /locus_tag="ACHA6Y_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988150.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2296..2511)
                     /locus_tag="ACHA6Y_002072"
     CDS             complement(2296..2511)
                     /locus_tag="ACHA6Y_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM8988151.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2735..3535
                     /locus_tag="ACHA6Y_002073"
     CDS             2735..3535
                     /locus_tag="ACHA6Y_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8988152.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3554..5422
                     /locus_tag="ACHA6Y_002074"
     CDS             3554..5422
                     /locus_tag="ACHA6Y_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8988153.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5415..5906
                     /locus_tag="ACHA6Y_002075"
     CDS             5415..5906
                     /locus_tag="ACHA6Y_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM8988154.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5894..6448
                     /locus_tag="ACHA6Y_002076"
     CDS             5894..6448
                     /locus_tag="ACHA6Y_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM8988155.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6466..>7460
                     /locus_tag="ACHA6Y_002077"
     CDS             6466..>7460
                     /locus_tag="ACHA6Y_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM8988156.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2650 a         1198 c         1423 g         2189 t
ORIGIN      
        1 ttttttattc aaaaaaaata gcacaatggc aaagatcgta agagagatca taagccccaa
       61 tgaatgacta aaaagaaaag cagaatcaca tgccatatca tcccatattt cttcaagaaa
      121 ataaataagc gggaaaaagg gaaggaatag tagaacatcg atccagcacc atttttttct
      181 tcttttgaaa taacataaaa caataaacat gaacgtgcag ggaagtaaaa acaaagcttt
      241 actcataact aacaaaatac cagctccttt tatttttttc tcccccacat tcaaagagta
      301 acaatggtat gtacttaata atagaaaaat ggatcaactt gtatgaataa cagctgaaaa
      361 gatctgcacc aaataaaaaa cttccagcca aaactgactg gaagtttatt tttttctgaa
      421 atattctaac acaaatatga aaaagatggc cgaagctaat aagccgattt cgtagcaaaa
      481 caatgagata ccagagtcgt aataataaaa tcctctaatc ttatggataa aatagatcat
      541 cggacaagca gataagagta agtatttgtt aggccttttg aataagtaaa agaaagcact
      601 ggtcattatg ataaatggta tccaaaacaa aagtttactt aagacaatca aatgatcaac
      661 tcccatattt tttaccaaga gagatcataa catctttatc gtaaaaacaa aatatttttt
      721 tgctcatttt gaaaatttaa acatactcgt tcttatccaa atatataaaa atcgcccctc
      781 attcatcgag aggcgataag ttctaattta actcaatttt ttttgtttct tttggctgat
      841 aagctgtttt aggtaatacc aatttcagca caccgttttt aaaggttcca gtgatttttt
      901 cttcatcaac attaggaaga tagaatgaac gtttgtaaga gaccgaatgt ctttctttcc
      961 gtacatagtt gccatcttct ttatcttctg tatgggactc atgattggcg gagatcgtca
     1021 aagtattatc tgaatattca actgtcgtgt cttctttgtc acagccaggc atatcagcag
     1081 ttagtttgta ttctttttca ttttcgacta aatctacttt tggataactt gaaacaccta
     1141 aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc agggaacatg tcgcgaatgc
     1201 taggtacatt tgccattttt gatcatcctt tcaataagta ttttttattt atccctattt
     1261 acgtaataac tctattttga aaaaaatgca agtataatgg ttagctttgt gaatatttca
     1321 tctaatacga tacaaaggct atctttctaa tttgaaaaag ggatcattaa aataataaga
     1381 agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc caaatataga aaaattgaat
     1441 ctagaaaacc gcccgacaat aaatttcatt aactcagttg accgaaaatg aagtagatac
     1501 caaaaattta tcaagagagc aagtagagaa gtgagtatat tcataagaga aatagctgta
     1561 acgaattatt aggttaatgc aaatgcttat ttatactccg tataactttc tcagaaaaaa
     1621 tgataccaat aatgacttgc tagtactgta aatccgtata atatcatcta atcaaaatgg
     1681 tgttccattt gcatctgtca tcgtaatcga tccttcgggt gacactcgat aaaaaccaac
     1741 gcttccagat cctccttggg aacggatttg tttgctggaa gctgtgaaca aataatctcg
     1801 atcaattttt tgataaaagc taataacaat atcgtcactt tgtaattcgg aacggtgggc
     1861 aataagaaat agaagtgctt tgtcagagtt ttctaatgga agagaaggta cagtgctttc
     1921 ttgcatttca gatgttgaag aaatgctaaa cgactgaata cttgattggc tatgttgcga
     1981 atctacatta ccagtagaag tattagtaat ggaagtagta gaagtacttt tagttgaacc
     2041 aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca gaattctttg ttttatatcc
     2101 acaggcagaa aaaagtatta cagttcccaa caagcctgcg taaatccctt tatgcatgat
     2161 agctcctcct aaaatagatt atctttattc agtatatcac ttaaacaaag aaaagcagat
     2221 ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc atgaagataa taactttttt
     2281 aataattata gctttctaat atctttcatc aacttttcta gtaatcattg ggaaaaattt
     2341 caagcagtag atcgcacaaa tacctacgac gatataaatg atcttcgcaa aaatagttgt
     2401 tgaaccacca gcaatcatag ctactaaatc aagttcgaac aagcctacca acagccagtt
     2461 caaccctccc acaatcaata aagttaatgc aatagcatct aaagttttca ttagaattct
     2521 cctttcttaa atgataaaac aattatatat caaaaagaga aacattgctt atataacgct
     2581 caggaatttt agcaatctat gcaaaaagat gaaaattttt tttgctcttc cattagtaca
     2641 cattttttag ctgaagctgt aaatttcatg tcttaatttt ttttagtgag cttttatact
     2701 ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg gattggttgg atattgcagg
     2761 aaaagtagta attgtaactg gaggttcctc aggaattgga agaagtatcg tcgaaaattt
     2821 gttgaagcag aatgcacaag tagcgaattt tgatgtaact gaatgccgta tacagcatga
     2881 aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg gacatagaag aagggatata
     2941 taaagtaatg aaacatttca agacgatcga tggtctagtc aataatgcag gaataaatat
     3001 tccttctctt ctaatagata gaaatcatcc aaaaagcaag tatgaattat cagaacaagt
     3061 ttttgataag atgatcgctg tcaatcaaaa aagtgtatat ttgatgagcc aagcagttgg
     3121 aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat ctttcttctg aatcaggatt
     3181 ggaaggatca gaaggacaga gctgttatgc tgctacaaaa gcagcgatga acagttttac
     3241 tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt gtggtaggtg ttgcaccagg
     3301 gatactggaa gaaaccggtt tgagaacaca agaatatgaa gaagctcttt cttatacgag
     3361 aggtataagc gtcgaacagc tgagaaacgg ttacagtcga acaagtacga tccctctagg
     3421 aaggagtggt aagctgcaag aagtggcgga tctagtctgc tattatcttt cagaaagatc
     3481 aagttatatc actggagtca cgactaatat ttcaggaggt aaaacaagag gataaaggat
     3541 aggggggacg cacgtgacgc tagtaaacag atggtaccaa ttattgaaat tactagtcac
     3601 tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc aaaaccagta atcaaaccat
     3661 aaaaaaagat attggggagt tgaatgaaga aattgccggt attgcaaaaa tcatccaaaa
     3721 aaacaaacat tttgaacttg aaatttatga ttttgatttt tttgatgaga tcatgcaagg
     3781 aaaattgaaa acagccagtg atttcaattc ggcatccaaa agaatcgctt atttactaaa
     3841 taggctgatc gaaacagagg atttcttacg tatggatgat ttgtctgaag aagtcggtgt
     3901 cagcagagga acagtggtca aggatctaaa gacattaaaa gagatgatca atgattttga
     3961 tgtaaagatt acaggcacac caaataaagg actaagaata ataggagatg agctagaatt
     4021 acgactactt ttattttatt ttgtttctcc ctacttttca gagacgaaaa cagaacagtt
     4081 tctagaagaa aaacaagcaa tcaaacattt tcagaagaag acgaatgcta gtaaacaaga
     4141 aatcaccctt ttaacgaaag ttatcggtat ttctttgaat agaatctcta gtaactatct
     4201 gttgaagaag cccatcaatc gttacagtgg gtgttttgaa tatatccaag aattcaatga
     4261 actgatcaat cagttggaag aaatctatga actaactttg agccaatatg aaaaagattt
     4321 tatcagtttt cctttgaaca tcatcaacac aagtataacg atgataagga aagctaactc
     4381 tgatttgcgc ttatatttcg accccatgat gatgcgtatt cgacgattag atctcattga
     4441 tctggatgag tccttcttat atcaagagat gaaacagcac ctctctttat tgataaatcg
     4501 ggtaattttt cgttatcgat tgactgattt attttatggt gagatagaaa aaaagtatcc
     4561 tttttcttac caaatcgcta gcgaatgcat acaagcatta gaagctatct tgcaacataa
     4621 gatctctaaa gtagagatca gctacctaac gttgtatttg gagttagcat ttcgatatca
     4681 aaaagagcat ttttcctcta aaaaagtagc cattgttcat acgacaggaa aaggaacagc
     4741 attaatgatc caaagacaaa tcaaaaggat attggggagg gatatacaaa taacacatgt
     4801 ttcagaagaa caatatgaaa aagtagatct aaacaaatat ttcgctgttt ttacaacgat
     4861 tccgttaaaa aatgtaaatg aagagacacc ggtgatccat ttgactaatc tttttgatga
     4921 ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag cgattagccg ggtttcattc
     4981 tactgttttt ttctatcaaa tatggaaaga aaaaaagacg tatcaagaat atttaaacaa
     5041 attagtcgct cctttgatca gaggctatta tgtggatgaa gaatttgaaa aaaggatctt
     5101 gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa tttgcttttc ctcacacaac
     5161 caaccagcta tctcaacaca ttgttttatc agtagggcta ataactgatc aaggtttagc
     5221 agaaacagga ggaaatggag ttattttttt atttgccata cctgaagaaa tgacggctga
     5281 agttgagaat gaattatttg agctatacga tatcctattt gaactcataa aaatgggaat
     5341 agatgagttt attgaattag caccgacaca agaatcattt ttaaaactaa tcaaaagtaa
     5401 aggagagaga cttcatgaat gatatgtatc tattcatagt tttcgcagct tcagcttatg
     5461 tgctgcaagc agttatcagt tggaagcaac taaagcattt caatcaaaca tatagcgaac
     5521 tgcgaagaaa aggaaaagta gcgattggta aacgacctgg gaaaatccgt tcaggcacga
     5581 ttgttatgct agctatagac gatcatgatc aagttttaga agtcagaaaa atgcaagggg
     5641 ttacatcttt ggcaaaattt cattcattac ctgtctatgc gggtgaagat atccattaca
     5701 tcgatcgtta ccatccctca gttcgagcgg aagatcgttt gacacaagca gctttagaaa
     5761 atgccagaga agtatactta cgaatagagg caggaaatga aaaaagtgaa caaccttcat
     5821 tgccttttat tgctatgctc aataattttc ggatatggaa aagtacgatc aaagaaaaaa
     5881 ggagtgacaa acgatggata tcataacaaa atttgctgag ggatttatga ggctgttcca
     5941 aacaggtgca gaaacattca taagctggat gacaggaatc gtaccagttg tcttgatgct
     6001 gatggttgca atgaatacgc tgattgcttt tttaggtgaa gaccgagtaa cgaaagtagc
     6061 caaattatca gcgaaaaatc cgcttactcg ttaccttgtg ttaccgttct tatctgcttt
     6121 tatgttggga aatcctatgt catttacaat ggctcgtttc cttccagaat actacaaacc
     6181 aagctattat gctgctcaag ctcaattttg tcatacgagc aatggtgtat tcccccatat
     6241 taatcctgga gaattgtttg tatggatggg gattgctcaa gggatcgaaa ccttagggct
     6301 aaattcgatg gagctggcta tccgatactt gcttgtggga ctgctgatga actttattgg
     6361 tggttggata actgatttta ctactggatt tgtatgcaga cagcaaggaa tcgttcttag
     6421 caaaaaagta gagctttcag ttgattagag aaaggagagt tttaaatgta tggtgatatt
     6481 aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga ttattaggcc gacaaaagat
     6541 aaacacaagt tcatctatgt cacaggtgga ggtgagaggc caatgatcgt agataaaata
     6601 gaagaactga caggtatgga gtctatcaat gggttcaaaa catcaatacc tgatagtgaa
     6661 atcgcgcttg caatcgttga ctgtggaggg actcttagat gtggaattta tcctaaaaaa
     6721 gggataccaa ccatcaatat cgtttccaca ggaaaaagtg gcccattagc acaatatatc
     6781 actgaagata tttatgtttc agcagtaggg ttagaacaaa tacagccact aatggaaact
     6841 gatgataaca aagaaacagt aaatactgca tctccagata aaaagtacaa atatgatact
     6901 tctaaaaaga taaccgagca aaaagcagag cagcaaaact ggatagcaaa aattggaatg
     6961 ggcgcgggaa aagtagttgc tacattcaat caatcagcaa gagaagcagt agatacgatg
     7021 atccggacag tgatcccttt tatggctttc gtttcccttt tgattggtgt gatacaaggt
     7081 tctgggatcg gtaatgtatt tgctaacatt atggcacctt tagcaggaaa tgcagttgga
     7141 ttaacaatca ttggctttat ttgttcactg ccatttttat ctcctttgct tggtccaggg
     7201 gcagttatta gccaagtgat tggaacattg atcggtgtag agattgggaa aggaaatatt
     7261 cctccccaat tagcattacc ggcactattt gctatcaata ctcaaaatgc atgtgatttt
     7321 attccagtcg gtctaggttt ggaagaagca aaagctgaga cagtagaagt aggtgtacca
     7381 tcagttcttt attcaagatt tcttaacggc atcccacgag ttatcattgc ttgggtagca
     7441 agttttggat tatatagctg
//
LOCUS       DAYYHX010000134         7428 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000134 DAYYHX010000000
VERSION     DAYYHX010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.135"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..608
                     /locus_tag="ACHA6Y_002078"
     CDS             135..608
                     /locus_tag="ACHA6Y_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM8988157.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            605..2737
                     /gene="feoB"
                     /locus_tag="ACHA6Y_002079"
     CDS             605..2737
                     /gene="feoB"
                     /locus_tag="ACHA6Y_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM8988158.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2739..2873
                     /locus_tag="ACHA6Y_002080"
     CDS             2739..2873
                     /locus_tag="ACHA6Y_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM8988159.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            2967..3611
                     /locus_tag="ACHA6Y_002081"
     CDS             2967..3611
                     /locus_tag="ACHA6Y_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8988160.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3798..4991
                     /locus_tag="ACHA6Y_002082"
     CDS             3798..4991
                     /locus_tag="ACHA6Y_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8988161.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            4984..6711
                     /locus_tag="ACHA6Y_002083"
     CDS             4984..6711
                     /locus_tag="ACHA6Y_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM8988162.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            7105..7302
                     /locus_tag="ACHA6Y_002084"
     CDS             7105..7302
                     /locus_tag="ACHA6Y_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8988163.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            7304..>7428
                     /locus_tag="ACHA6Y_002085"
     CDS             7304..>7428
                     /locus_tag="ACHA6Y_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8988164.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQ"
BASE COUNT         2425 a         1269 c         1643 g         2091 t
ORIGIN      
        1 ataattgaca ttctcaatta ccgagagtat tctatgtgag aatgattctc atttatttgc
       61 gtaacacgta agaaaattag aacatataat gaatgatttt aatacatgat ttatttcaca
      121 agagaggaat caatatgcag accttagcgc aggcaaagca gaatttcgtt tatcaagtcg
      181 aacgaatcga aacagaagaa aaaacaaaac gccatttgca aaatatcggg atcgttgccg
      241 gaagtaaaat cgtcgtcgtc aatttatcag gcaacaatgc cattttactg gtaaaaagtc
      301 atcgagtggc agtaagtcgg gaaatactag gtcaaatctt tgtcaataaa aagactgctt
      361 cctctgaaag ctggacttct cttgatcaat taaaagcagg agagactggg atcgtcgtag
      421 caatccacgg acaaggcgca gtgaagcgac ggttaatgga tatgggattg acgcgaaatg
      481 taaaaatagt tatccgaaaa ctagcccctc tgggagatcc aatcgaactg acagtgcgag
      541 ggtatgaact gacgttacga aagtcagagg ccgaactaat cttgatacaa agggaggaaa
      601 aagaatgaat atcgcgttag tcggaaatcc aaacagcggg aaaactagct tgttcaatct
      661 cttgactggt tcgaatcaat atgtagggaa ttggccgggc gtaacagtag aaaaaaaaga
      721 aggatcgttg aaaaaaaatc cgaacattca tattcaggac ctacccggca tctattcttt
      781 gtctccttat acaccagaag aagtgattgc aagagattat ttggttcagg aacagcctga
      841 actgatcatc aacattattg atgctaccaa tttagaacga aatctttatt taactacgca
      901 gttattggat ttagacattc ctatgatcat tggtctgaat atgatggatg tcatcaaaaa
      961 agaaggaaaa aaaatcaatc tagaaaagct ttcctatggt cttggtgtac cagttttacc
     1021 aatcagcgta ttgaaaaagc aaggaatcga aaaactaatc gaaaagattc aggagtctgc
     1081 aaaaaagcgg ccttctattc ctgttccctg tacatatgat ccacgtttag aagcagcttt
     1141 acatgaaatt gaagatgtac taggagaaca aagcggcaag aaacgctggt atgcaatgaa
     1201 atattttgaa cgagatcaga aggtaagaga ggattacgag atagcagttt ctcagcaaaa
     1261 agagatcgag caactgatcc agttaacaga gaaactattg gacgacgata gtgagacgat
     1321 tctggtcaat gaacgttatg aatttattac ccaactatgt acactctgtg ttgtgtcaaa
     1381 tgattctttc caattatcga tgagtgacaa aatcgatcag attgcaacga atcgttggtt
     1441 agccttgccg atcttcgcat ttgtgatgtg gctgatctat tatctggcta ttcaaacagt
     1501 aggaacaatg gggacagatt ggatcaatga cacattgttt ggtacatggt taccagaaca
     1561 tgttggcaga ttgttagctg aatggcaagt agcgggatgg atgcaggagc tgatcttgaa
     1621 cggcatcatc gcaggcgtgg gagcagtgtt aggatttcta ccgcaattga ttatattgtt
     1681 tttatgcttg agctttctgg aagactgcgg atatatggcg aggatcgctt ttgtcatgga
     1741 tcgacttttc aggaaattcg gtctttcagg aaaatccttc attcctatgc tgattgctac
     1801 aggatgtgga gtgccaggcg tcatggccag tcgcacgatt gaaaacgagc aagatcgtcg
     1861 tttgacgatc atggtcacaa ccttcatgcc ttgttctgca aagttgccga ttattgcctt
     1921 agtagcaggg gccttttttc ctcatcaatc ttgggtagct ccttctgcct attttcttgg
     1981 tatgactgca attattttct ctggaatcag cttgaaaaaa acacgtttgt ttgctggaga
     2041 tacagctcct tttatcatgg aattgcccgc atatcatttt cctcaatggt cagcggtcct
     2101 aagacaaacc tatgaccgca gcaagtcttt tgttaaaaaa gcaggaacga ttatctttgt
     2161 ttctagtatc gtgatctggt tcagctcttc ttataatttt tatgctcaac ctgtttctga
     2221 agaccaaagt atcttagcct ttttcgggcg aatcctggca gttgtttttc agccgcttgg
     2281 ttggggaaac tggcaaggaa cagtcgcagc gattacagga ttggtcgcta aagaaaatat
     2341 tattggcaca tttggtattt tgtttggtca tgcggaagta tccgaaaatg gcagagaaat
     2401 ctggcaagtg ctacaacaca catatactcc cgcagcagct tattcattgt tagccttcaa
     2461 tttactttgt gcgccttgtt ttgcggcaat tggggctatc catcgggaaa tgaatgccaa
     2521 gaaatggact tggatcgcca taggctatca atgcggttta gcttatttag ttagttttgt
     2581 gatttatcag ttgggacact tattggaggg tggacaggta gcgttaggga cgtatcttgc
     2641 tattttactg atgataggac tcgtttatgt tttgttccgt cagccaaaat ccaagaacca
     2701 gttttatact atattgtcat tagaaggaga ggaataggat ggcgacattc ttattaagtg
     2761 tgttaatctt tggaagtgca ggcgcaattg tctatactcg tttaaaatcc ggtaagaatt
     2821 gtgatgactg tcaaacagct tgcccagtaa aaaaggaaca gatcaagcga tgatcagtcg
     2881 ttgcaagagg ctattctccc cttttttgct tattttgcta gagcatagta agatgaggga
     2941 aagtaaaaaa agaaagagga aagaaaatgt ctggaaagaa aaaaatcgtc cgcccgcgct
     3001 accagcagat tgctgttgat ttagccgaac gtatcgttga aaaccgatat aaagtgggtg
     3061 agaagatcca tgcccgctca acacttgcca gtacgtacaa tgcttctcct gagactaccc
     3121 gcaaggctat caatgtgctg gtagatcttg gaatcatgga agtacggcat ggaagtgggg
     3181 catttgttgc ttcgaaggaa aaagcaaaag atttcctatt gcaatatcaa gatgtacaat
     3241 ccatccaaga tacaaaagca gaaatcatgg caagtgtgga gaagcaacaa gatgagctga
     3301 atcatttttc tcaattattg gatcagctgg tcaatcaaac gaagcgaata cataagatga
     3361 atcctttact tccgtttgaa ttatctttat cagaaaaagc aatgcatctg gaccgttcaa
     3421 ttagcgagtt gaatatctgg caggcgactg gcgccacagt tatcgcaatt tctcataatg
     3481 aagaactgct tgtttcacct ggcccctatg ctaaatttac ggcaggggac atcgttcatt
     3541 ttattggaaa tgaatataca ctacagcgta tgacaaactt tttttacccg gagcatcagc
     3601 ttgcagaatg attgtgtgaa atattaaaaa aatattaact tggctaaacc ttttatttac
     3661 aaagatttag ccaagttttt ttcagtttgg acaagcaaat ttctttcatt tttgacaaag
     3721 tgactacatg atgttaacat agtgtggtca cttttttcag aaagaaaaat gaatttacat
     3781 aaaaaaggag aatttgtttg gtaaaagtag aagtaaaaca tttaacaaag atatttggta
     3841 aaaagacaca agccgcttta gatatgatga atgatcacca gccgaaaacg gagattttaa
     3901 agaaaaccgg tgcaacggtt ggtgtgtatg acgtgaattt tgacgtaaaa gaaggagaaa
     3961 tcttcgtgat catggggctt tccgggagtg ggaaatccac attgatccgt ctattgaatc
     4021 gtctgattga gccaacttcg ggcagtatct atattgatgg agaagatgta tcgaagttaa
     4081 gcaaagaaga attgcgagaa gtccgtcgtc ataaaatcaa tatggttttc caaaacttcg
     4141 gtctgtttcc acatcgaacg atcttggaaa atacagaata cgggttagaa gtccgtggtg
     4201 tacctaaaga agaacgacaa gaaaaagcag aaaaagcatt agaaaattct agtttgcttt
     4261 catttaaaga ccaatatcct agtcagttat ctggtgggat gcaacaacgg gttggcctag
     4321 ccagagcttt agcaaatgat cctgagatct tgctgatgga cgaagcgttc tcggctcttg
     4381 atccgctgat ccggcgagaa atgcaagacg aattactgga cctgcaagcc aatgttcaaa
     4441 aaacgattat tttcattacc catgatttga atgaagcttt acggattggg gacagaatcg
     4501 ccttgatgaa agacggcgaa atcatgcaga tcggaacagg tgaagaaata ttgaccaatc
     4561 cagcaaatga ttatgtacgt gaatttgtgg aagaagtcga tcgttccaaa gtgcttacgg
     4621 ctcaaaacat catggtacct gcattgacga cgaatatcga atctgatgga ccaaacgttg
     4681 cattgacaag gatgcgtaat gaagaagtca gcatgctgat ggctgttgac agaaagagac
     4741 acctgaaagg gatcatcact gcagatcaag cgttagaagc taggaagcaa aaacgtccat
     4801 tgatcgactt cctagatgaa aatgtgacgg tgattgggaa agacatggtg gtaagtgata
     4861 ttttcaatat tatttatgac tcaccgactc cacttgcagt tgtagaggat ggaaaattaa
     4921 aaggtgtcgt catccgaggt agtgtgattg aagcattagc agaaacaagc gaggtgagtg
     4981 aacatgaata atctattgaa ctatcaatta ccagtggcaa actgggtaga aaatatcaca
     5041 gaatggttta cgacaacttt ttctggccta ttttcatttt tacaaacgat tggacaggca
     5101 gtgatgtcgg ggattacaaa tttactgtta gtgattccag ctcctttgtt tatcttgtta
     5161 ttgacggtag cagctttctt tatttctaag aaacgtccag gacttacgtt gttcacatta
     5221 atcggtctat ggtttattta taaccaaggt ctatggaatg atttgatgaa tactgtcaca
     5281 cttgttcttt tatctagtgt gatctcgatc atcattgggg taccattagg gatcttgatg
     5341 gcaaaaagca gtaaagccca aagtattatc aaaccgatcc ttgactttat gcagacaatg
     5401 ccaggattcg tttatttgat tccagcagtt gcatttttcg gtatcgggat ggttccaggg
     5461 gtatttgcct cagtcatctt tgctctgcca ccaaccgttc gttttacgaa tttagggatt
     5521 cgacaagttc ctaaagaatt agtagaagct tctgattcat tcgggagcac aagtcgtcaa
     5581 aaactattca aactagaatt gcctttagct aaaagtacga tcatggcagg gatcaatcaa
     5641 acaacaatgc tgtcactttc aatggtcgtt attgcctcta tgatcggtgc acccggctta
     5701 ggacgcggcg tattatcagc attgcaaaga gcacaagtcg ggaatggatt tgtcaacggg
     5761 gttgcattag ttatcttagc aatcatcatt gaccgtttta cacagcattt gaaccagcca
     5821 aacaataaaa aagctgcagg agcaacaaca cagaaaagta aaaaacgtca aggactgatc
     5881 attggtgctg tagtagtagt gatcttaggt gcgattggta tagggagttt ttcatctgcc
     5941 aaagaaacaa aacgtatcaa cttatcttat gttgaatggg atacagaagt tgcttcaaca
     6001 aatgtagtcg gtgaagtatt gaaacaaatg ggctatgatg taacgatgac gccattagat
     6061 aattcaatca tgtggaaatc tgtttcaaat ggcgagtcag atgccatggt ttcagcttgg
     6121 ctacctaaaa ctcatggttc acaatatgca caatataaag atcaagtcga agacctaggt
     6181 gccaatttga caggagcaaa agttggttta gcagtgccag cctacatgga tgtaaattcg
     6241 atcgatgagt taaccgatca ggcaggtaag aaaatcatag gaatcgagcc aggagctggt
     6301 gtagttacgg ctgctgaaaa cacgatccaa aaatatgaca acttgaaaga ctggaaagta
     6361 gagacatctt cttcaggagc aatgaccgtt gcactaggtc aagcgatcaa aaaacacgaa
     6421 ccaattgtag ttacaggatg gacacctcat tggatgtttg ccaaatatga tctgaaatac
     6481 ttagaagatc ctgaaaacgg aatgggaagc gaagagcaga tccatacgat ggttcgaaaa
     6541 ggattgaaag aagaccagcc agaagcttac aaagtattag acaatttcca ttggtcagaa
     6601 aaagatatgg aaaaagttat gttggaaatc aacaatggaa aagatccaca gcaagcagca
     6661 aaagactgga tcaaagagaa ccaagaatta gtcgaaagct ggaaaaaata aaaagacaga
     6721 cacgaaataa atggagcttc caaaattgga tcaaatatcc aaatttggaa gctccatgtt
     6781 cgtctctatc ttttttgtct ataaaaaaag agaatgcgta gctcatcttc gaaacgttca
     6841 aagacgccga caaactatta tgaatttatg ggtgcgagtc aaagagatcg atcgtaggta
     6901 tgaaagacta atgtgattaa agtttattat gtacgaagtg cattctcaaa aaatattaaa
     6961 gaatatacat agtttatata cttaaaagtg attaaattct tattcaggaa ttattttgct
     7021 acgtttcaag aaggaattgt tttttgaaat ttaatgaatg ttagaataat agtctaaaaa
     7081 cattaattta ggggtgatta gattatgaaa catttaaaaa ttttgtctat taaagagaca
     7141 caacttatct atgggggtac cactcatagt ggaaaatatt atggaaatgg agtgtattgc
     7201 actaaaaata aatgtacggt cgattgggcc aaggcaacta cttgtattgc aggaatgtct
     7261 ataggtggtt ttttaggtgg agcaattcca gggaagtgct aaaatgaaaa aaaatgctaa
     7321 gcaaattgtt catgaattat ataatgatat atctataagt aaagatccta aatattctga
     7381 tattcttgag gttttacaaa aggtatattt aaaattagaa aaacaaaa
//
LOCUS       DAYYHX010000135         7397 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000135 DAYYHX010000000
VERSION     DAYYHX010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.136"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..730
                     /locus_tag="ACHA6Y_002086"
     CDS             <1..730
                     /locus_tag="ACHA6Y_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8988165.1"
                     /translation="LFSSDQAFLITKNTILYNVAFILLNLLISVVFAIIMSELRNKRL
                     VKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFI
                     LVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTIL
                     TILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQS
                     VVGFVLLMITNTIARRFDSDSALF"
     gene            742..1662
                     /locus_tag="ACHA6Y_002087"
     CDS             742..1662
                     /locus_tag="ACHA6Y_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8988166.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1703..3160
                     /locus_tag="ACHA6Y_002088"
     CDS             1703..3160
                     /locus_tag="ACHA6Y_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8988167.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3505..4119
                     /locus_tag="ACHA6Y_002089"
     CDS             3505..4119
                     /locus_tag="ACHA6Y_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8988168.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4112..5836
                     /locus_tag="ACHA6Y_002090"
     CDS             4112..5836
                     /locus_tag="ACHA6Y_002090"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8988169.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5833..7284
                     /locus_tag="ACHA6Y_002091"
     CDS             5833..7284
                     /locus_tag="ACHA6Y_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8988170.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2524 a         1231 c         1471 g         2171 t
ORIGIN      
        1 tttattttct tcggatcaag catttctcat cactaaaaat acaattttat ataatgtagc
       61 atttatcctt ttgaatctgc tgatctcagt cgtatttgca atcattatga gcgagttgag
      121 aaataaacga ctcgttaaag tttatcaaac gatgtcctta ctaccttatt ttctatcttg
      181 ggtcattatc agttactttg tttacgcttt tttaagtcct gacaaaggaa tattcaatca
      241 gtggatcact ggacatggag gagaagctgt caattggtat aatgaaccga aatattggcc
      301 gtttatcttg gtgttcattg gtacatggaa agggattggt tataacagca ttatttactt
      361 tgcatctgtt atgggaatcg atccgactta ttatgaagct gctatggttg atggagctag
      421 caaatggcag cagatcaaac atgtgacgat accacaactt attccactaa tgacgatttt
      481 aacgatttta gccgtaggaa acattttccg agctgatttt ggcctgtttt ataacattcc
      541 taggaattcc ggtgcattat atgatgtgac tcaagttttg gatacgtata tctacaatgg
      601 cctgacatcc acaggagatt ttggtatgac ggctgctgca ggactttacc aatcagttgt
      661 cggctttgtc ttgttgatga tcacaaatac gatcgcccga cgttttgata gcgattctgc
      721 attattctaa ggaggaaaaa aatgaaaaaa aagaaagtgg agagagtcca agtccgctct
      781 ttcaataaga cggctaattt tttctctaat ataatgattg cactctttgc tatttcctgt
      841 attttgccat ttatatttgt tatcgccatc tcttttaccg atgagtccat attgtcgact
      901 cacgggtaca gtttttggcc agaaaagttc agtatatttg gttatacgta cttgttcgac
      961 cagatgcagg ataaaatttt ccaagcgctt ttcgtcactg tacttgttac tgttttaggt
     1021 acactgatca atagcacggc tacttcttta tatgcttatg cgatttcacg gtcaaacttt
     1081 ccattccgtc gtttttttac cgttttttgt ttgattacga tgttgttctc accagggatg
     1141 gtggccaatt atcttgttat gacaaatctt cttcagttaa aagatacgat ttgggcattg
     1201 atcttgccaa tggctgtcag tcccttcaat attattgtga tgcgcacatt ctttaaacgt
     1261 tcggtctctg attcgattat tgaatcggca cggatcgatg gagctagcga actgcgaata
     1321 tttgttcaga tcgttttacc gttagctgtt ccaggtattg cgacaatcag tttatttgcc
     1381 gcattgggtt actggaatga ctggttcaat gctttactat acatccaaga tgataagcta
     1441 gttccactac agtatctttt gatgaaaatc caaagtaata ttcaatatct gacacaaaat
     1501 tcaggagcag gaagccaatt gacaggcgga ttagcctcag ttccaggaga atctgccaga
     1561 atggcgatcg tagtcatctc gacgttgcct attgcgatca gctatccatt tttccagaag
     1621 cattttgtca aaggtctaac aatcggcgga gtaaaagaat aaagaaacaa aaatataaat
     1681 cataaaatta ggaggaagaa aaatgaagtt atggaagaaa ctagccttta caggtgtttc
     1741 agcgttgttg ttgggaacat tagcagcatg cggaggttct ggttctaaag accaggcaga
     1801 agctagcaat tcggacactt tgcagatgta tcaaatcgga gataaaccag ataatttcga
     1861 tcaattgatg gaagtagcaa acaaacgaat cgaaaaagag atcggtgtaa aagtcaatat
     1921 caattacatt ggctggggcg attacgaaaa gaaaatgaac gtcatcattt cttccgggga
     1981 aaattatgat atcgcctttg cgaacaacta tgtttccaat gcccaaaaag gagcctttgc
     2041 tgatttgact gaattagcgc ctaagtacgc tgaaaaagct tataatgatt tagatgaagc
     2101 atatattaaa ggaaatctgg tcaatggaaa attatatgct ttcccagtca atggaaatgt
     2161 ctttgctcaa caagtactta cttttaacaa agctttacta gataaatacg atctttcaat
     2221 tgatggaatc caatcttacg ctgatgcaga aaaagtcttg aaggaattcc atgaaaaaga
     2281 accaaacacg gcagcttttg ccattggaca aggcttcaaa gtccaaggga actttgatta
     2341 tcctttaggg aatacgttac catttgctat tgatctggat ggagatgatt cgaaaatcat
     2401 caaccaatac gataatgaaa cgtatatcgg actgttacgt acaatgcata gctggtacaa
     2461 acaaggactc attccaagtg acgctgcaac gagcaataag gattatccac tagaaggaaa
     2521 tacttggcta atgcgtggag aaacacaagg accgtatgat tatggcgata caattttgac
     2581 aaatgcagca aaacaagaac ttgtctcaaa agcaattact gttccactaa aatctacagc
     2641 tcaagcacaa atggcaaatt ttgtcgtatc aaatacttca aaaaataaag aaaaatcagt
     2701 agaacttcta gggctgctta acagtgatcc agaattattg aatgggcttg tttgggggat
     2761 cgagggagaa gcatgggaaa aagtcgatgg ttcagatgat aaaatcaaat tgctagatgg
     2821 ttatcagcca aatacacata tgtctgcttg gaatacagga aacaacaaga ttctatatac
     2881 acaagagtct attacggatg acatgattgc caaacgtgat caatcgattg ctgatgcaga
     2941 aacttcaccg attttaggat tcagcttcaa cactgacagt gtcaaaacag aattaagtaa
     3001 tatttctaat gtcatgaatc aatatttaga tggattgaat acagggactg ttgatccaga
     3061 tgaaacatta ccgaaattga aagacgcttt ggacaaggct ggctatgata aggtattgaa
     3121 agaaatgcaa aaacaatatg atgagttccg acaagaataa taatgaataa agacaaaggg
     3181 gttgtgacaa aagacgtgtc acaacctctt gttccgaata aacggtgttc aaaagccagt
     3241 ttctcggtat taagctaaat atatgtaaaa atgtggaaag ccatattcac aaaatttttc
     3301 ttactactcg aaactaagcg gctatttcac aaactcttgg taaacggtgt tcaatcagct
     3361 aaccctcggt attcgctcac aaaacgacaa aatatgagga actatttttc cgtttgtttc
     3421 tctaataatc aggtcaaggt gctgattgcc caaccttttt ttgtataatg agaaagtata
     3481 aacttaatgt ttggaggaga agatatgtta gggaaggttt tggaagagtt atttatccgg
     3541 atctgggtca ttataaaact aaccttgtat ttctggatat atacatttgc cggcgggatc
     3601 atctttggat taggtgcggc atggaaaaca gtcaatgaac tattttattt gtacggattc
     3661 gagtataaag aaatcacaat aaaaagaggc tggaatatat ataaaagaaa ttttttaaga
     3721 ggcaatctgc tatttagttt atttttatct ggtactgctt tattgagtta caatttatat
     3781 ttatctgttc aaatcaaagg gctcattttt ttagctattg attttatttt actttttgcc
     3841 ttgctctgcc tatttgctac ttatcaatac agtatgattt tagatagtga atatacgatc
     3901 tctgttccta atctgctaaa gctaagtttt atttcggttt tttccagctt ttcgtctttt
     3961 ctaaaaataa ttattggtag cggtataatt ttaggcgtga cttggcaatt caaaggattg
     4021 atcctgtttg gtgtcattgg tcttttgacg gtttggaatg gtacgatgac aacacattgg
     4081 cgtgaagaat tagataaaca gttggaaagt tatgaataag tttcggattc tattttcaaa
     4141 aggaacctta ctaaatcgtc tgttgcggaa atacgcggtt atcatgatcg gtgtaacgat
     4201 gactgctacc gttattttta gtatccatac ttgggaacaa aatcaaaaac aggcagaaaa
     4261 catgacaagt gatgccgttc aaagcacaag cagaatgctg aatgataaaa caaccttgag
     4321 taggatcatc aaaaaccagc ttgtagggaa ttccgaaaag atcgaaaatg tgacgaccta
     4381 cttaacaaaa cccatagatc agtatctgat gtatgtatac gaacagcaaa acagtaccga
     4441 tgaattagtt tctttcccaa atcaaatcaa agatctctac gcaaattatg aagaactaag
     4501 tgcgatatat atcgtgttga atcaactacc ggagtattat gaatcttcta gagaaaacaa
     4561 aggtggggaa ataaaaaagg ggacaccaaa attactagac gccttttata tcaagttgcc
     4621 tatcacacaa ggaggagctg agtcaggaag tatttttatt ggttttcaaa agaaagaact
     4681 agatatgatt ttagaaaatc taacgtcatt caatggactc tctttataca tgatttctgg
     4741 aacgacaaat cgactataca cattccatga taataaaata gctaaagaaa cgtttgaaag
     4801 acaagaaatg atcatcacag agagtctgaa gaaaaattca caattgccga ttgacgatct
     4861 ggaaaaaaac aattttgttc aacaccaaga actgtctgat gattatcgta tattagctgt
     4921 actagataaa aaaagtgtaa aaatcgaaac gggaaaaaat cttgccccgc tgatcattgg
     4981 tatcattatt ttagacaata tactgcttat ctttctttat aaaacattca aacgatattc
     5041 acagcaagta tctatcgtca tgcaggccat ggatcaaaca gcagcaggga atttagagac
     5101 gcggatcgac ataacaaaca cggaatatga actgaaggaa ctttcaatcg gtatcaacga
     5161 aatgttggat agcatcaatc agtttgtaga agacatctac aaattagaaa tcaaacagca
     5221 agacgcacat atgcgggcgc ttcaagcaca gatcaatcca cactttttat ataatacatt
     5281 agaatatatc cgaatgtatg caatcagcga aggaagtgaa gaattagcag atgttgtgta
     5341 tgcgttttcc gctttgcttc gaaacaatac gaatcaagag aaaacgatca ctttaaaaga
     5401 agaactggac ttttgcgaaa aatatgtcta tttgtatcaa atgcgttatc ctaatcgagt
     5461 agcctatcat tttatgatcg atcctgattt ggaaaagatc gaagtaccta aatttgtgat
     5521 ccagccatta gttgaaaatt attttaaaca cggtattgat tttactcgat tcgataatgc
     5581 gctaagtgtc aaagtcttgc aggaaggcaa acgggtaagg atcatcatca aagataatgg
     5641 aaaagggatg actgaaaaac ggctaaaaca gattgaagaa aaactgtctc atcctaaagt
     5701 ggaacttcac ggttctatcg gtctgcaaaa cgtgaatgaa cgtttacgtg ccagcttcgg
     5761 cagcagttat tatatgtcgc ttgagaataa tgagacaggt ggactgaccg tttctattac
     5821 attcaaggag ggatgagcgt gtatcaagtg ttattggtag atgatgaata catgattttg
     5881 aatgggttaa aaaagataat tgattggcaa agtttaggct ttcaaatagt agcaacagca
     5941 gagaatgcca aagaaggact agctgtactt gaacagcgac agattgattt agtagtaacg
     6001 gatgtgacaa tgccagaaat aaatggactg gaatttattg aagcggctca aaaagaacgt
     6061 cataattttg aatttatgat tttatcagga taccaagaat tcgattattt gaaaggaggt
     6121 atgcaattag gcgccgtgaa ttatttgatg aaacccgtga ataagttcga attggttgaa
     6181 agcctcaaga aaatcaaaac acggttagat cagcaaaatg aacaaaaaaa tcaacaagaa
     6241 atctatcaag aactgttatt cagtcaatgg ttgaatgaag aattggatga aataagtgaa
     6301 gaagaaataa taggacaaat aagtgagaag cagcatagga tagtattgat tcaactggca
     6361 agaactcatg atactcttat caatagatgg ttgcaagaac ggcaacaaat cttttactat
     6421 caacgaaatt acggagaccg tgtattgatc acgttgttgt tggaggcaga tgaagttgat
     6481 ggcttctgtt gttttgttca agaaaacttt cctgatcagg aatggctgat cagtatcggg
     6541 gaagagacag attctttgga caaaatccct gaaacattcc aacaagcgaa agacaatcta
     6601 cagttgcatc aattttatgg agataaagac gagcatgttt tttacgcagt acaagcagaa
     6661 acaaaagatc aaaccatcga tttttcaagt ttcagacgcg tgctgcaaaa taaacaattg
     6721 gaagtcgctc aaaaaatgat cactgatttt tttgaacagt ttcagttagc agccatgatg
     6781 ccggaagata tacgatattc cagtttttta ctgtttatgg agattcaacg ggaattgatt
     6841 gatctggaag atgaggaata tttacaagga atcgaaaaaa tccatcaggc aaaaacggtg
     6901 caagaacttc agcagctact tctgtcattc atcaaaaagc atcagagaca gaaaaagtac
     6961 agtgacaatg tggaaaaagt aatcgtaatc ctgcaccagc attatcaaga accacttaca
     7021 ctcaaagaag tgtcagaaaa cctgcattta aatgtcatgt atctaggaca gctgttcaaa
     7081 aaagaaacga aaaaaagttt ttcagcttat ttgaaccatt tacgtatgga aaaagcgaaa
     7141 caactgcttt tgcacagtaa tcaaaacatc aatgagatag caagtgagat tggctacaac
     7201 aacaccacgt atttctctaa attattcaag aaaatcgtcg gcagatcccc taaagagtac
     7261 cgagaaaaca gtggagaatc ataaaagaaa aagccaatgt aatgaaataa cttataaaaa
     7321 attgtctaga aatctaattt tgggctgttc ttattacttg aggcaaggca gctttttcat
     7381 gacctctcga taaacgg
//
LOCUS       DAYYHX010000136         7372 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000136 DAYYHX010000000
VERSION     DAYYHX010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.137"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..1034
                     /locus_tag="ACHA6Y_002092"
     CDS             177..1034
                     /locus_tag="ACHA6Y_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8988171.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1027..1929
                     /locus_tag="ACHA6Y_002093"
     CDS             1027..1929
                     /locus_tag="ACHA6Y_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8988172.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1962..2312
                     /locus_tag="ACHA6Y_002094"
     CDS             1962..2312
                     /locus_tag="ACHA6Y_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8988173.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2361..3284
                     /locus_tag="ACHA6Y_002095"
     CDS             2361..3284
                     /locus_tag="ACHA6Y_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8988174.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3362..4255
                     /locus_tag="ACHA6Y_002096"
     CDS             3362..4255
                     /locus_tag="ACHA6Y_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8988175.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4293..4856
                     /gene="amaP"
                     /locus_tag="ACHA6Y_002097"
     CDS             4293..4856
                     /gene="amaP"
                     /locus_tag="ACHA6Y_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8988176.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4870..5058
                     /locus_tag="ACHA6Y_002098"
     CDS             4870..5058
                     /locus_tag="ACHA6Y_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8988177.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5071..5973
                     /locus_tag="ACHA6Y_002099"
     CDS             5071..5973
                     /locus_tag="ACHA6Y_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8988178.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6005..6247
                     /locus_tag="ACHA6Y_002100"
     CDS             6005..6247
                     /locus_tag="ACHA6Y_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8988179.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6503..6874)
                     /locus_tag="ACHA6Y_002101"
     CDS             complement(6503..6874)
                     /locus_tag="ACHA6Y_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988180.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2704 a         1242 c         1459 g         1967 t
ORIGIN      
        1 acctttgaat aaaaagccat gaaaaacgaa tagttagcgc ttaccgatgc gttttgtgat
       61 aaaaaaattt taaaatattc aatgaaaaca aagcatatat tttgctttaa aaccaaatga
      121 ggaatattct agatgtagaa caggttagaa aaagattaga aaaaggagtg attttgatgg
      181 agaagagata cggcgtagtt cctgtggtaa ctgcttcaga cgaaaattat gctccttact
      241 tgagcgtgat gatcgcaact gcattagaaa attgcaataa aacaagacgc ataaaatttt
      301 atgtgattga cgatggtcta tcagaatata gtaaagaagg attggaagaa acggtaaata
      361 aatattcaag taatgcaagt atccaattcc tgacagtgga aaaagatatc tacgaagact
      421 tcctagtcag tgaccatatc acaactaccg cttacttaag aatttcttta ccaaacttat
      481 tagctaaaga ggattataag aaagtccttt acttagattc agatgtgtta gtactagacg
      541 atatcgtaaa actatatgat gagccgttaa atggaaaaac aatcggcgct attatcgatc
      601 caggccaagt gaaagctttg gaaagattag gaatcgattc cgatgacttg tacttcaatt
      661 caggagtgat ggtgattgac attgatcaat ggaataaaaa agaaattaca gaaaaaacta
      721 tccattacct cagtgaaaat ggcgatcgaa tcatctatca cgaccaagat gctttgaatg
      781 ctgtacttta tgaagattgg gaacaacttc atccaaaatg gaacatgcag acttcgctga
      841 tttttgaacg tcatccagca ccaaatgaaa aatatgaacg tcagtataaa gaaggaaatg
      901 aaaaaccttc gatcgttcac tttacaggac atgataaacc ttggaatacg ttgaaagatc
      961 atccgtatac caatttatac ttaaaaaaat tagcacacag tactctaacg aaagtaggcg
     1021 agatcaatga ataaaaaaga aatagcagta gtcgcaagtt gtaacacaaa attcgtacca
     1081 catttagctg cattatttgt ctcagtatta gataattgca acccgtctaa atttgttcgg
     1141 ttctatgtga ttgacgatga tatcgatttt gaaagtaaac agcttttacg cttctcagtg
     1201 aaaaacgcac gaatgaactc agatgtggaa ttcttgaaaa tcaataaaga attctttaca
     1261 aatgtggtaa tcagcgatcg tattccagaa acagcttatt atcgtattgc gattccggaa
     1321 ttgttccgcg gcacagaagt tgaacgtatc ttatatatgg attgcgacat gatcgcttta
     1381 caagatatct ctaaattatg gcgtcttgac ttcggtgatt caatcgtagc agctgttgaa
     1441 gacgctgggt tccatcaacg actggaaaaa atgaaaattt cagctaaatc aatgcgttat
     1501 ttcaactcag gtttgatgct gatcaatgtg aaaaaatggt tagatgaaaa tattacgcaa
     1561 aaagtattag atttcatcga acataatcca gaaaaattac gattccatga ccaagatgca
     1621 ttaaatgcta tcttacatga ccgttggtta ccacttcatc cgcgttggaa tgcacaaggc
     1681 tatatcatgg caaaagcaaa aaaacatcca acagcagcag gtgaacgtga atacgaagaa
     1741 actcgcaaca atccttacat tatccatttt tcaggacatg tgaaaccgtg gagcaaggat
     1801 tttgaagggc caacgaagaa atattacgaa aaatatgcag ggatgacggc tttccgttgt
     1861 gtagctaaat tccctaaata tccaaaatat gcaaaagttc aaccaaaaac agatgtagtt
     1921 gttaaataat agaagacaac aactgaagag ggggagtagg aatggaaatt acaatgtcta
     1981 aaaatgcagt agagacgtta attgaaaagg ttggggaaaa tacaaaaatc gcactcgcgc
     2041 ttatcaatga ttccgatcca tttctaaggg acaaaggtgc ttttgcaaaa ggcagctttt
     2101 ttcaaatcat tccttttgtt tcggaatttg gagaatatgc gactaaaatc gagcatccat
     2161 tattggacat ctatacatct aaattagagc aaaattattt cggtaaacga ttgaacatgg
     2221 acttcaacaa acagcttgac agcttttcat tagaaaatga aatcgcagta ctcgattata
     2281 atatcaaatt aaagaactgc ttcttcagct aatagtttcg agaaagtcca aaaaggaggt
     2341 atacattgag atactacttt gtggatgaag aaaagaatgt aatagaaaca gatgaaaaaa
     2401 aatataattg gctcgtactt gatagtgcaa agcccgaaga gattgagcaa gcaatcgaag
     2461 aattcgagct tcctgatgat attttcgtag gaaccgaata tccggaagaa gtttctcgga
     2521 tggagcgcct ttatgagacg aaactagagc atccattttc tttggtagtg attaatctag
     2581 atcagtcaga taatcgtatc gaaaaaaaat taacgccaat atcttttgta ctttccgata
     2641 atctcttgat tacttgtatc gatagaaaaa ccgaatttgt ggatcggctg ctgacaaagc
     2701 atggaaatca tttaaactcg tttgaaaaga tcatcacgta tacattatta gatatttaca
     2761 cccattacgt gaaagaacta agagagatga aaaagcgaat tgacgctttg gataaagcag
     2821 ctcgtaaaac aacggaaaat gaagaactgt ataaacaggc agatcttgaa agagacatcg
     2881 tatatatcga tcatacctta agagatcaac gggaaacaat ggatcaatta tggaattctg
     2941 aaactttcat caaacgttta gacgatgaac agctactcta tgatgtccat ttgagacagc
     3001 gacaaacaga aaaaatgatc gagatctatc gagatttatt agagagtatt ggtgatctat
     3061 tcaacgggat gatggacaat aatctaaatc atatcatgaa atatctagat tcagctgcat
     3121 taatcatttc cgttccggcg ttgttcgcag ggatttgggg aatgaataca ggaggattac
     3181 cgggtaaaag ttctgctcta ggctttatac tcgtagtctt tggctctttg atagcagcag
     3241 tagttttagg ttaccattta taccggaaag attatacaaa ttaaagaatc tatttaaaac
     3301 aggtatcaaa caaagaaatt tgttttggca atacaattta aaaactagga ggaatacagc
     3361 aatgacagaa ccaaaattaa tcgacccgcg aaacttgtac catacggaga aatttcctaa
     3421 acaagatcag gatacaccag ctttacagca gaatatgatt cctgttcctg attgtggaga
     3481 agaatcctat gaaggcaata atcaactaga aaaccgccgt gtactaatca ctggtggaga
     3541 ttcagggatc ggtcgtgcag cagcgattgc atttgctaga gaaggggcag atatcgctct
     3601 tcaattcttc ccaggagaag aaaaagatgc agaggaagta gcaaaatacg tgaaagatgc
     3661 aggacgtaag attgtcttac taccttttga tcttcgtgac gaacaagcac ctaaagaaat
     3721 catagataaa gccgtgagtg aacttggcgg gctagatacg atggtactaa acgcaggaca
     3781 gcaaatttct tgtcagtcta ttgctgagtt accacttgag caagtcaaag acacatttat
     3841 gataaacatc atcgctatgt ttgctttagt gaaagaagct gttccgcata tcccagcagg
     3901 aggagcaatc gtaacaacaa cttctgttca agcattcaat ccaagcgaac atttattaga
     3961 ttacgcagca acaaaagctt caatcgctaa ctttacgatc ggtttagcaa aacagcttgc
     4021 acctaaagga atccgtgtaa atggtgtagc acctggtcca atctggacac cattacaact
     4081 agacgaagga caaccttctg attcgatccc tgaatttggt cagcaaagtt tactagaacg
     4141 tgcaggtcag ccaggagaat tagcaccagt ttatgttttc ttagcatcga ataaagcaag
     4201 ttatgttaca gcccaaattt acggagtaac tggtggagaa gcgatcaact tataagaagt
     4261 tgatcgtttc acccctatga ggaggtgcga aaatgaataa aggtgtaaaa acgatcggag
     4321 tgatcggaac attacttttg ctatctgtgc ttttgtttgc gatactatta agtgcaacct
     4381 atccattgcc ttttcagcta gagaagtttc gattcttttc catcactaat tcatacgtgc
     4441 aacaatatgt attttggctt gccgttgcat ttgctatttt gacaattatc acgttacttg
     4501 ttttcctgtt ttatccaaaa acaatcggaa actttctact gaaacatggt gatggggagt
     4561 tgacgttgga taaaaaagca attgaaggaa tggttcgttc tcacttgcat gaagatgaat
     4621 tcatccattc accaaaagta aatgtcaaag caacgaaaaa caagatcgcc gtcaatgtaa
     4681 aaggggagtt gaaacgaact tcagcactca ttggtaaaac aggaacattg atgaaagaaa
     4741 tcgaaaatga agtacaacac atattgggaa caaaagagcc agtgaaagta tcggtgaaat
     4801 acacaggctt tgaagaacca gctaaacgaa gcaatgaccg caaacgagta gaataggggg
     4861 ggaccaaaaa tgaaagaact attttcgaca tacaagctcc ctatcatctt tggggtactt
     4921 ggattgatat tagccgtttt atggatcacc gtgggctttt tcaagacctt attgatcttg
     4981 atcatggcag cgatcggcgt cgctatcggt ttttatctga aacaaacgaa aatcttagat
     5041 cactatttta aatcttagga ggaaacatcc atgaataaca ataacaaaaa taataacaat
     5101 aaaatgaaca accaaaaacc agtgtctgca catccagacc caacgacacc aaaccaatca
     5161 aatggtgtga acgatgaaaa gaacaaaaca gcaccagtat ctgcacatcc agatccagta
     5221 gtccctaacg ttcaacaagg agtagaagag aagaaacatg atgcagaaaa gcatgcacct
     5281 catcatccag gaactccaga atctaaagtt ccagaacaac atgatgcaat cgatccttct
     5341 aaacctgtgg aagagcaaaa acatccttct gaccatgcga aagcacaaga aaaacttagt
     5401 tctgaccaca caaacggaca aggaaaactt ggttctgatc atacaaataa ccaaggaaag
     5461 catgattctg aacaggaaga ttcaatgcgc ggtgagctga attatgaaga taaagttgtg
     5521 caaaaaatca tcggaatcgc aattgaacaa gtagacggtt tattatctgc aaacggtggt
     5581 ttcttctcta atgtggcagg aaaacttgtg aatacagata atgtaacggc tgggatcgaa
     5641 acagaagtcg gtaaaaaaca agtagcagtt gatatggatg tcattgtgga atatggcaaa
     5701 gatatcgaaa aaatctttga agaaatgcaa gaagtaatcg gcaaagaagt aaaaaatatg
     5761 acacatttag aagtgatcga agtcaacgct aacgtagtgg acatcaaaac aaaagaagaa
     5821 tttgaaaaag atcaagaaac ggtgcaagac aaagtaactg acgcagcgaa aaaaaccggt
     5881 gaatttgctt cgaatcaaac agataaagta aaatcagcag ctggaagcgg cgcacaaaaa
     5941 gtaaaagaaa atacagaacc acgggttcag taataaggaa gtaaaacatt aggaggaacg
     6001 aaacatggga tttatttggt cacttatcgt cggaggaatt atcggagcaa ttgcaggagc
     6061 aatcacgaat aaaggttctt cgatggggat tattgcaaat gtcgtagcag gtttagttgg
     6121 atcagcactt ggtcaagcat tattaggttc ttggggacca agtattgcag gaatggcaat
     6181 cgtgccatca ttgattggtg ccatcatttt agtactaatc gtatcattct ttgctcgaaa
     6241 actgtaaaaa gacttttaaa aagaggtcgt gaaaaagctg ttcttcatca agtaataaga
     6301 acagccaaac aaaaatagct tctcatattt ttcatttggt tgggcatatt atcgaagatg
     6361 caagcttttg aacactgttt atacggaata agaggctgtg acaagacttt tgtcacagcc
     6421 tcttatttat gcgggcaaat tcccaattag atttttaaaa gtttttcctt aaatgtgtct
     6481 ttattgatac agagaagctt ctttaataaa acaatgcctc cctaaatttt cttgagatag
     6541 gaaaggtgaa catacaaaat aatacgacca ctttgaaaac ccagatgatc cacaaatgga
     6601 tcgtatgata ctgatgaagt aaaaagcaat ggatcagaaa ctcaaagaga ttgaaaaaga
     6661 caaggaagaa ataatatctc ttttttactc ttgtctgttt tagccaataa tagctcgtaa
     6721 agtaatctag acaaacaaat agaagtaaac aaagtttcgt aagagcagca atcgtatcat
     6781 gttgctctgc tagacgattg acgagcagga aaaatgttgg taatggaata tagatcacga
     6841 gtaatgaaag aaaaaagtaa acaataaatg gcatactatt cctcctaatt gtgtgagttc
     6901 gccctcattt taaaataacg atatttgttt gtgaagcttt ttcgtgtcgt aacaaatgtt
     6961 atctaatgta tttttaaata aataatcatt attatcaaaa taatgttaaa aataaaaaag
     7021 aattgtccat cactgttatt caaatcattt tatcaaaact acatagtaaa tgttaaaaag
     7081 caacatgaca taatatatag aaggctttac agtatatatg ggagcttact aaaaaagaat
     7141 ggagtaagag ttaacacaat gggccattag ctgaagtttt aagcacttca aggtatgaaa
     7201 cagaatggcc tgtaatacta catgattatt tagactgctc aaactgctat cagtctggtg
     7261 gaatgaataa agtattatac taaaagcaga tccaactaca acgtaacagg aaaccagttg
     7321 tatcaggaaa agtgatagta catggagaag aaaaggcccc ctttgtatga gt
//
LOCUS       DAYYHX010000137         7363 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000137 DAYYHX010000000
VERSION     DAYYHX010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.138"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..470
                     /locus_tag="ACHA6Y_002102"
     CDS             15..470
                     /locus_tag="ACHA6Y_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM8988181.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            550..678
                     /locus_tag="ACHA6Y_002103"
     CDS             550..678
                     /locus_tag="ACHA6Y_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988182.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            940..1209
                     /locus_tag="ACHA6Y_002104"
     CDS             940..1209
                     /locus_tag="ACHA6Y_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988183.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            1609..1863
                     /gene="rpsT"
                     /locus_tag="ACHA6Y_002105"
     CDS             1609..1863
                     /gene="rpsT"
                     /locus_tag="ACHA6Y_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM8988184.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(1952..2998)
                     /gene="holA"
                     /locus_tag="ACHA6Y_002106"
     CDS             complement(1952..2998)
                     /gene="holA"
                     /locus_tag="ACHA6Y_002106"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM8988185.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(3036..5309)
                     /locus_tag="ACHA6Y_002107"
     CDS             complement(3036..5309)
                     /locus_tag="ACHA6Y_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM8988186.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(5302..5841)
                     /locus_tag="ACHA6Y_002108"
     CDS             complement(5302..5841)
                     /locus_tag="ACHA6Y_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM8988187.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(5977..6195)
                     /locus_tag="ACHA6Y_002109"
     CDS             complement(5977..6195)
                     /locus_tag="ACHA6Y_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM8988188.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(6192..6437)
                     /locus_tag="ACHA6Y_002110"
     CDS             complement(6192..6437)
                     /locus_tag="ACHA6Y_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8988189.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(6553..7281)
                     /locus_tag="ACHA6Y_002111"
     CDS             complement(6553..7281)
                     /locus_tag="ACHA6Y_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM8988190.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         2253 a         1466 c         1245 g         2399 t
ORIGIN      
        1 gaggaatata tttcatggaa agttggcttt ttttaggaat tattttactt gtagcaattt
       61 tagctaaaaa tcaaagtctg atgatagcgg cagcttttgt tttgattatg aagctcatac
      121 cagcctcccg ttcctttttc ccttggcttc aaggcaaagg aatcaatatt ggggtgacga
      181 tcatcacagc ggctattcta atccctatcg caacaggaga aatcggtcta cgtgatctat
      241 tagaagcctt taaatcccct attggctggg tagctgtttt ttgcggtggg ctagtggctg
      301 tcttatcttc caagggtgta ggattgattg cttctgatcc acaaatcact gttgcacttg
      361 ttttcggcac gattttaggt gttgtcttct taagaggaat cgccgccgga ccggtcattg
      421 cttctggtat cacttactgt atcttacaag tatttgcttt aggaaaatag aacaaatttt
      481 gtctatttta ggagattctt aacaccccca atttactgat ccgtgatata ctgacaacga
      541 ggtgatacaa tgttcgaaaa attaaagtat ttcataggaa caatcggctg gtttcttcta
      601 gcggtacttt tgattctggg cttagcttat ctattatttc ctgatttgat acaaaatgtg
      661 tttgatactg ctttttgatt gacctattaa aacgatatag gggatgctcc tgcgatgcag
      721 gggctttttt taatcaatgt tttgtaataa tttaggccgt gccaatgaga cttcctatag
      781 aattcgatgc tcttttttct ttttggctct tctgctcttt tttgttatac tagattacag
      841 tggacaaaag aaagaatgat cgcatccact tcaacgaata tcaaaatgga agaattagtg
      901 cctgttttca ggttactttg aaaagaaagg aagacgcttg tggaaacttt tgaaggtctt
      961 ttaattgata cgtacgtttt acaagaacag ccaatgatgc tgactgggat caacttgcag
     1021 cttgaggacc gtgtgatcaa tggcctgctt aaagatcgct atttggcaaa atatatctta
     1081 cgtttgcccc taaataaata tgtgctggaa gtaagtggca aatggaaccg tcggaaccag
     1141 ttggtcataa aaaaaatgtt gataaaaaat atcgatgaat acattaaaat aatcggtaca
     1201 ccagaatagc ctttactgat caaaggatac tggttataaa ataactgcag tttcttaata
     1261 gaaaaacaaa gaacaacagg gaatcgtatc ccttttcaaa aaaaactttg caaacggttc
     1321 tctttgttat ataataactt agtaccaaac attcccattc aaactttgtt taaatagata
     1381 aataaaaatc tagtttggtt tgacgctatt gcctcccaca atagcgtctt tttgattttt
     1441 ttcagcttgt actatccgtt tttactgaaa aaaaatccta ataaaagact gttaaggaaa
     1501 aaaacttgtc atgttcatat tgcaaagcac gctgccttta ggtataataa tgaatgttga
     1561 attatgcggt aacacgcgta agaacttaag ttatcggagg tgaaactaat gccaaacatt
     1621 gaatctgcaa tcaaacgtgt tcgtacaagc gaaaacgcta atgtgaaaaa ttcatctcaa
     1681 acaagcgcta tgcgtactgc aatcaaaaaa tttgaagatg ctgtagcttc aggtgctgac
     1741 aacgtagatg cgttatataa agaagctgtt aaagctattg atatggctga atcaaaaggt
     1801 ctaatccaca aaaacaaagc taaccgcgac aaatctcgtt taagcaaaaa aatcgctaaa
     1861 taaaagaatc ggcttcggcc gattttttta tttgtcaaaa aaaggacaaa aacaaacgac
     1921 cttcccgcat gctttgccct cgtctttctt cttatcgatt tcttaacaac tgccctgtgc
     1981 gcaaaacgaa tagttgaaaa cttaattctt tttcgatttg tcccgttttg atttgataat
     2041 ccatctcaac taattgatca aacaactgta ccagcaaatg ttcatcgaat ttttttactt
     2101 cctgcatggc aagttttaca cgatatggat ggattttcaa tgtttcagca atatttgcct
     2161 gctgataacc aatctttaca agaaattttg tctgtaaaaa taaacgtaat tgagaaagca
     2221 aaatcgcatt aattttgatt gtttcttccc cttgcataac taaatcttcg tataaacgca
     2281 aagcttgttc cgtcttccct tttaaaatat attgggtcat gtcaaaaata ttatgttcta
     2341 acgttttagg aactaattct tctacttctc ttaccgtgat ttgctgttga ttttgtccat
     2401 aaaggaccaa tttctgtaac tctctcatca ttttggataa atctaagtcg gtcaacctca
     2461 agaataaatc aattgcactt cgttccattt tgattgagga attctctaag gtattcatca
     2521 gatattgacg tacttctttt tcattcaact gttggacatt gatgataatt gctgtttttt
     2581 tcaattgttt cgttactttt ttccgttcat ccaacttttc atatggcgca aagaatacta
     2641 atacagtcga ttctaatggt tctttcaaat aagccgtcaa taaatcagta ttttgttcga
     2701 ttccattgtt tactttttca cctgttaaaa aataaggatt ctctacgaat actaaccgtt
     2761 gatctccgaa aaacggcaac gtctctgctt cagctactac ctcatctaaa cttgcatttt
     2821 ccatgtcaaa acttaaaaaa tttaagtcat cttcgccctc taattggatt tttttgacaa
     2881 tttctgtccg aacttgatct tggagaaact tttctgttcc taaaatcaag taacaagatg
     2941 caagtttttc tttacgtatt ttttgtaact ctgcttgtat actcaaatta ctcaccactt
     3001 tcaaaaatta tcctaccatg agcagatgag aattactagt ctatcattgt ttccactagc
     3061 ccatgaggat gaaaaactgg atgccagcga taggtgatca ttccttgttg atccgttcgc
     3121 catgttttta tctgattttc ttctagtgtc tccaatactt cgctgtgcgg gtgaccataa
     3181 cgattattta cgccgcagga tataatccca tgttttgctt caatatggga aagaaaatct
     3241 gtgttacttg acgtacggct accgtgatga cctaatttga ctacatcggc tttcagatta
     3301 ggataaatca aaagcaattg tttttccatt gcttgattca aatctcctaa aaataaaaag
     3361 cgcctattgt tatattccat ccatagacca atcgaatctt catttcttcc ctctcccttt
     3421 tcaaaaggag tcaatacata aagatcataa taaccgctga tcctgtcccc atgtttgatt
     3481 tcaatgatct cagtaccatt tttttgagct tcagataaat gcctttgtaa tggctcattt
     3541 cgtccagcgc cccaaccgat ataaattttt ttgatagtaa aatgctgaga taacgtgggt
     3601 aattctccca catgatccgt atcgtcgtgt gtcaagatga gtttatcgat tacacttatc
     3661 ccctgacctt ttagatatgg aacgatattt ctttcagcag gaggacgtat cataccttgc
     3721 gcccactttt ctctttcaaa gtgtaactga cctccagtat cgatcaaaat cgtttctttt
     3781 cgaaaaggac tttgcaaaaa gatactgtct ccttgaccga catcgataaa agcgatcgtt
     3841 gaagaaaagc gtaaaaatgg caaagtcaaa aaaataaaag aaaaaagacc aacataaaac
     3901 aagtgtttca caggttgttc tttcaaccta tccatacaaa tcagtaatcc gaaaatatac
     3961 atcccagata gcacaagggg caaacgtcct ataatgagag acgaccctga agaaaaatcg
     4021 attattcgct caaataagat taatccctct tctatcagca aaagtaaagt ttccggcagc
     4081 cagcgattga agagtgtgag aaataacaca ccaggaagaa aaagaaactt gaataccggt
     4141 gcaatcagta atgtaaaaag actacctaaa ataggccact cataaaaatg ccatacaatc
     4201 aacggcatcg tgatcatagt aaacaggacg tgagaggccg tttttttcaa ccaatctcca
     4261 tcaaaagaaa tcaggataaa caaaaaactc atgaaaaaac taagctgtcc acccgcatga
     4321 aaaagaaatc gaggatcaaa tagtaaaaga agccaaaaga caactgcaaa taagtccatg
     4381 gtggaataat gcttattcag caatttgact agcaatcgca ataaaaacag caaactagca
     4441 cgcaaaacag aaatagaact tcctgcaata gaaacataac agatagttag tagactcaac
     4501 ggaataatac tgacgtgtag tggccatccg atacgtcgaa acaaataata cagccaccct
     4561 aaataaaatt ggatgtgtag acccgataga ctgaacagat gcagcaaacc actctcccta
     4621 aaaatttctt ctggttctct gaatcctgca tctttatagc cccataataa agaattaata
     4681 tatacacttg ttttttgcgg aaattcagat tggacaaagg agataaaaga tcctcttttt
     4741 ctctgaagcc agtttcgcca gtgattttgc atggtgatct gttcaatctt ttgaatttgg
     4801 aaccgtccag aaattctatg gttcctgaag taagcttctt ggtcaaaatt ataaagattc
     4861 cgcttttttg ctggagagat gaactcgcct tcagcagtca attctattcc ctttcctcgg
     4921 aagttaagcc atgttttcaa ttctgcttct gtgtttactt gataagaaag ctggagcttt
     4981 tgtttattct ctgataacct tccttccatt gtcaaaaaat taccatttgc acgaatcgta
     5041 tctgtaaata cagtaatttg cgcagatgtt gttttttcct ctaaaaatga atactcttgt
     5101 agcctataag aaatggatac cgttatgcta cacaaaatac acaagtacgc gataaaacgt
     5161 ccttctttta gcgaaaacca taaaaaaatt ccggcaagaa gcaaacgata accccaattg
     5221 tcagtgtaat acaacaaaca gataatcatc gacgtaattg ccggaaaaat caaccgattt
     5281 cgaacatgcc tttctaaaac gctagacatg ttttcctgtt aattcatttt gaagttttga
     5341 aaaatgctgt gagtctaaag caacttggtg tacagataca cctaattgtt cgatcaacgc
     5401 ttgggcataa ggatcatttc gatagtcatg gagataattg atccctttca caccagcttg
     5461 caacaaagcc tttgtacagg ccaaacatgg aaaatgtgtg acatagattt ctgaaccttc
     5521 cgtcgggata ccaagttttg cacactgtag gagtgcattc atctctgcat ggatcgttcg
     5581 caagcagtgt ccatccacga cgtaacaccc ttcatctatg cagtggacat ctccactgac
     5641 tgctccatta tagccgccag caatgatcct tttttctcga acgagtgtcg ctcctacttc
     5701 taaacgtgta cacgtactac gtaaagataa cagtactgcc tgagccataa agtactggtc
     5761 ccaaggaatg cgttcattat gtgacttatc agtaactttt tctttattcg tatcgcatgc
     5821 ttttgaagat ttttctaaca tgttttatat tcctccgttt tttcaagttg attaaaaagt
     5881 actcattgct taaacaaaat attctttaaa tataacgaat catcctgtct gacaaaagat
     5941 cctacccttc actgccgaaa tgaagtccgt catccattag cccattataa aaattcttcg
     6001 aatccatttg ttcaagaaat tccgtataaa aatcgatact tcacaatcat ggcgttttgc
     6061 tacttctgtg agaacagaga tatattcttt gatcaacttg ttattccgat atttttgttt
     6121 tgtctcatcg actgctggtg gcgtgataaa gaaagcatga tcagaactat agtctttttt
     6181 cagctgaact atcatccact gcagattttt tggtacgttt ctttagaaag ttgtttatgc
     6241 tctgctagat cattcgtccc aatgagaata aatactttat ctgctgtata gctaagtaca
     6301 tctttggcaa agcgttctcg tagagcttga ctgttattgc cgctgacagc cgtattcata
     6361 atatgaatcc cagatatttt attttggaga tagacattca gcattggttt ttttaatgtt
     6421 tctttgcgaa caatcaagct atttcctatc caaaaatact acctcctcgt cctgccaagc
     6481 tcttagaatt ggattattct tagtatccat tagcgtccat ttattgctcg tgccagtcga
     6541 ttttgaatca ttctatttaa ttgtaacgaa gttttttagt ttttcaactg tcttttcacc
     6601 gataccagaa atctcctgca ggtcttccac tttctggaag gatccgtttt cttctctaaa
     6661 tcgaatgatt tcttgcgctt tgactgatcc gatcccagga atctgctgaa gttctgatgg
     6721 agtggctgta ttgatattta ttttgggagg ctcggaatct gctgtaggct gttttccttc
     6781 cgaggatgcc aactgatcaa gatgttctgg cagctcttca cctacagcag ggatatatag
     6841 gacttgctga tcttctatct tcgcagaaaa gttgattcga tcgctatccg cttcttctaa
     6901 taatccacct gcacgcttta gtacatcata gacccgttct tctgaagaaa aggaatagat
     6961 accaggctcc ttcacacttc ctttgatatc ggtgtacatg acctttggac gagcatcctc
     7021 ttctacatcg tttccttcag ctgatggatc ttctccactg gaactaaacg aagaaagtat
     7081 ttcttttgca gcagtctctt ctgtttctct ataaaatagc aaatttgata ataaaaaacc
     7141 acttgcgata caaaagataa gaactatcat agacatccaa atgatcaatt tgggtgaagc
     7201 tagtattttc ttgctgattt tttctatcat tgttcttttt tcaaagaatg accagtaaaa
     7261 atcattcatt actcctttca tttaaaattg aagaaacagt gagctctggc aaaatcgtcc
     7321 cttcaacatc taattacgtc aattttttct tttgttttta ttt
//
LOCUS       DAYYHX010000138         7348 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000138 DAYYHX010000000
VERSION     DAYYHX010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.139"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..2679
                     /gene="clpB"
                     /locus_tag="ACHA6Y_002112"
     CDS             70..2679
                     /gene="clpB"
                     /locus_tag="ACHA6Y_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM8988191.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2830..3516)
                     /locus_tag="ACHA6Y_002113"
     CDS             complement(2830..3516)
                     /locus_tag="ACHA6Y_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988192.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3494..3988)
                     /locus_tag="ACHA6Y_002114"
     CDS             complement(3494..3988)
                     /locus_tag="ACHA6Y_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8988193.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4008..5228)
                     /locus_tag="ACHA6Y_002115"
     CDS             complement(4008..5228)
                     /locus_tag="ACHA6Y_002115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8988194.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5375..7210
                     /gene="lepA"
                     /locus_tag="ACHA6Y_002116"
     CDS             5375..7210
                     /gene="lepA"
                     /locus_tag="ACHA6Y_002116"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM8988195.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
BASE COUNT         2425 a         1397 c         1497 g         2029 t
ORIGIN      
        1 cgatataata gtttttgtaa agatcaaaga aataagtgat cttaccttta attaggaggg
       61 tatacctgta tgaatattga gaaaatgact acgacgcttc aagaagcgat tgctgaagct
      121 caacagattg cggtcactcg aaaacatcaa gatatcgaca ttgcccatgt atggaaaatc
      181 tttttacaat ctaatcattt tggtcgaaat ttctatacgg atgccggatt ggatgtcgaa
      241 tcttttgaac atgaaattga tcgtctactg gatgagtatc cagttgtttc tggtggaaac
      301 gtccaatatg gacaaaacct cagccagaat ttctttcgat tattgaatga agcagaccaa
      361 atacgtgaaa gcttcggcga cgagtttctt tcaacggaag tcgttatttt agcgttgatg
      421 aaattaaaaa attatccgtt aactgtttat ttgaataaaa acggattatc tgaaaaagaa
      481 ttgcgtaaaa acatcgaaga gatgagagga ggagaaagag tgacctcaca aaaccaagaa
      541 gaacaataca aagcattaga aaaatacggc gttgatttag tacagcaagt caaaagcgga
      601 aaaatggatc caatcatcgg tcgtgatgaa gaaatccgtg atgtcatccg tattctttca
      661 cgaaaaacaa aaaataatcc tgttctgata ggagaacccg gtgtagggaa aacggcaatc
      721 gttgaagggc ttgctcaacg tatcgtacgc aaagatgtgc ctgaaaattt aaaagataaa
      781 acgatttttt cattagatat gggtgcattg atcgctggag caaaattccg tggtgaattt
      841 gaagaaagac taaaagcagt cttgaaagaa gtcaaaaaaa gtgatggacg tatcctcttg
      901 ttcatcgatg aaatccacaa tatcgtaggg gcaggaaaaa cagaaggaag tatggatgcc
      961 ggaaatctgt tgaaaccaat gcttgctcgc ggtgaattac atttgatcgg tgccacaact
     1021 ttagatgagt accgtcaata tatggaaaaa gacaaagcgt tggaacgtcg ctttcaaaag
     1081 gtattagtca aagaaccaac agtggaagat acgatcagta ttctgcgtgg attaaaagaa
     1141 cgttttgaga tccaccatgg tgttaacatc catgacaacg cattagtagc agcagctact
     1201 ttatctgacc gttatattac tgatcggttc ttaccggaca aagcaatcga tttgatcgat
     1261 gaagccagtg caacgattcg ggtagaaatg aactctatgc caacggaatt agatcaagtt
     1321 actagacgtt tgatgcagtt ggaaatcgaa gaagctgcac tgaaaaaaga atcagatgat
     1381 gcaagtaaaa aacgcttgaa aaatttgcaa gaagaattag ctgaacttcg tgaagaagca
     1441 aatgcgatga agatgcagtg ggaaacagaa aaagaagaag tcaattctgt ttcagcaaaa
     1501 cgtgctgaaa tcgataaagc aaaacacgaa ttagaagatg cagaaaataa ctatgattta
     1561 gaacgcgctg ctgtattgcg tcatggtacg atacctcaat tagaaaaaga attgaaggaa
     1621 ttagaagcta aagcaaaaga cagcgagatt aagatggttc aagagtccgt taccgaaaat
     1681 gagattgctc aagtcgttgg cagattaacg ggtatccccg taacgaaact tgtagaaggt
     1741 gaacgagaaa aactaattaa attgaatgaa acacttcata aacgagtgat tggtcaagat
     1801 gaagcagttg atgcggtcag tgacgcggta attcgttcac gggctggatt gcaagaccca
     1861 aatcgtcctc taggttcttt ccttttccta ggaccaactg gtgtcggaaa aaccgaatta
     1921 gcgaaagcgt tagctgagaa cttgtttgac tcagaagatc atatggtacg gatcgatatg
     1981 agtgaatata tggaaaaaca tgctgtgtct cgtctagtcg gagctcctcc aggctatgta
     2041 ggttacgaag aaggtggtca attaacggaa gctgtcagac gaaatcctta tacaatcgtc
     2101 ttgttggatg aaatcgaaaa agcgcatccc gatgtcttca atatcttgtt acaagtccta
     2161 gatgacggac gcttgactga ttcaaaaggg cgagtagtag atttcaaaaa tacggttctg
     2221 atcatgacca gcaatatcgg ttcacagtta ctgctggaag gcgtgactgc tgatggaacg
     2281 attccagaag cagtggctga acaagtcaac acgctattac gtggaaactt caaaccagaa
     2341 ttcttgaacc gaatcgacga cacgatttta tttacaccat tgagcctaga caacgtgaaa
     2401 ggaatcgttg acaaaatggt ggcgcaattg gcacaacgtc tggaacatca agaaatcctg
     2461 ctgacaatca gtgatgaagc caaaacgtgg attgctgaaa atgcttatga accagcatat
     2521 ggtgcaagac cattgaaacg atttatcact aaggaagtag aaacaccact agctaaagaa
     2581 atcgttgcag gacatgttat gcctaaatca aaagtaacga ttactttact tgatggacaa
     2641 cttcatttca aaacagaaga gctagaagaa atcgtctaat cttccattga gactataaaa
     2701 gaaaaaaggg gtctgacaca actagtgtca gacctccttt tttcaaataa acagtgttcc
     2761 agaagtagtt tcttcgaaaa ataagccgaa atttcttgaa gctgcccgct tctgtcacaa
     2821 cctcttttat taggtatttt tcactttttc taatttataa ctttcaaagt aacgctcatc
     2881 ttttttactt tgagaccatg caaacaatcc cattaaggca gcacctatcg tatttgtcag
     2941 caagtcgccc atcgtatcca tcaatgctgc tcgtccgaca aacagtgtgc catcagaagc
     3001 agcaaaacgt tgaagattca tgcctaagaa ctggtcgcat aagaattccc agaactccca
     3061 aaacacgccg caaagtccag caaacgcaaa gccgaataag aggaacaacc aaggactggt
     3121 ttttgatatc tcagcatctt tcatcaagta accaatcaat ccatagccaa gagctgtcag
     3181 gaccattgga ctcaccgcat gtaagatttt gtcccaaaaa gagatgatac tgatcaaatg
     3241 catccctgtg cctaaaaaga cagagatgaa caagaaaaac cagtaaaaca aaacgattgc
     3301 tgctggtatt ttaagtttga aaactttcaa aatgacttgt ggcatgaaaa tcaatgcaat
     3361 ccctaataaa gattccacga taaatgtgat tccttgagaa gtcgaatatt tttgtgtgat
     3421 aaattcataa aaattatagc agacaacgaa tacaccaaaa gcaagtaaag ctatttgcac
     3481 ctttttgatt tctttaatca ctgaaagtcc ctccatctaa aaatcttttt acttctgaaa
     3541 gcaactcaat ttctgaagaa accacttttc cgttcaattt gatcaaacct attgtatata
     3601 agttcaggta gtgaaattga ttttcagctg tttcttgtag tgcagcgatt tttttcgggt
     3661 gggttccgcc ttgctgtcga gtatcagtat ataagccgat cactgggatg cctttcgcat
     3721 aagccacacc gatttccgat gctaccccgg catcgatcgt cagcccatct agtaaagcaa
     3781 tcattaaatc actagccaac actttttctg tgtcagcaag agcaatcatt cgactgtctg
     3841 cataagcagt tttgtcattg attgcttcat tttcttgagg aagatagatc gaaagctcag
     3901 aagagagctc tctgatttct ttaactaact gttggttata taacaaatca cttttagcaa
     3961 acattggtgc ggcaaaataa attttcatct aaacatcatc ctttttatca aaaataatcg
     4021 ctaaagtaag cttccatgtt tggaatgata cgctctagtg acttcagtgt ttcttcatct
     4081 gtttctaacc gttcgattct tgccaaataa gaagcacggc gataattcat catcaagcaa
     4141 gtcaatgttt ggatatccag tcgaacggat tttcctatag gttcatttaa gacacggact
     4201 tgtccctgtt catcccatgt taaagcgaag acaccattat tccaaggagc gacaggatct
     4261 tcaatgatga aatgaaaagc atcagctgtt ccaacaaacg gaaaccgttg aaggaattct
     4321 tttacatcaa cgatccgagc cataaaatat ggttcgatct gctctttgat ctgactatct
     4381 tcaatcaaaa atgctaaagg ctcattttta taaatatctc catgtaccca gtagatcatg
     4441 gaaaaatgtg cggaaataaa gttccataag ccatttcgag cttcttgatc aagatagaac
     4501 atttctttta cgtgaaatat ttcttcagct acccagtaga acagaacacc tcttggttca
     4561 tggttgctat cgtagtagac agcggctgtg cgctcatctt cattttcgaa acgccaatac
     4621 tcttcccaat gaaaatcact gcggaataaa gcaccatggt tttgttgggc aaatttgtca
     4681 taaacggaaa atacgtctgg atgatcaact ggtaatcgct caaccatacc acttactggg
     4741 atttgtttag gtagctgtgt atctctgatt ttaaaggaaa gtttatcaga catgatttcc
     4801 catcctttac gccgatagta ggggatactg taaggataca aataagagat ccattgctta
     4861 ttcttgcgca tattatccaa tgcaacgata attagatctt gcatcaatcc atggttagca
     4921 tattctggat aagttccaac tccagtcaca ccacccattt tataacgtgc accgtgaata
     4981 ttgacttcac aaggatagat agcgatttga gagatcagtt tattttcgtg gaaccatccg
     5041 aacactttcg atacttccaa aatcggtttt ttggaacgga taaaagctcg tttattctca
     5101 aacccgcttt cctcgatgtc tgcttcagtc acttggaaca cataggaaag caattcattg
     5161 aattgatcga tatgttcttg agtgaccggt tttaattcaa gattttctct aaaatctttt
     5221 tgatccattt tttcatctcc atttattagc ttgataacag tatagcaaaa ttctacaaaa
     5281 aaaacttcta tagctgacta attaaaaact ctttaaatta ctgcttttcg ttggtataat
     5341 ggaaagtgct gattatatga aaagaggaga cgctatggat ataaagaaaa tgaaagaacg
     5401 ccaagagcag attcgtaatt tttcgatcat cgctcatatc gaccatggga aatctacatt
     5461 ggccgatcgt attttagaaa tgacgcacac tgtgacgtct cgcgaaatgc aagatcagct
     5521 gttagactcg atggatttgg aacgtgaacg tggaatcacg attaaattaa atgctgtaga
     5581 actcaattac acagcgaaaa atggagaaac atatatcttt cacttgattg acaccccagg
     5641 gcacgtcgac ttcacctatg aagtctctcg aagccttgct gcctgtgagg gtgccgtttt
     5701 agtggtcgat gcagcacaag gaatcgaagc acaaacattg gccaatgttt atttagcgtt
     5761 agacaatgat ttggagatcc tgccagtcat caataaaatc gacctacccg cagcagatcc
     5821 cgaacgtgtc cgtcaagaaa tcgaagacgt aatcggaatc gatgctagtg aagctgtatt
     5881 agccagtgcc aaatcaggga ttgggattga agatattctc gagcagatcg tggaatatgt
     5941 tcctgcacca agtggagact tagatgcacc gctaaaagct ttgatctttg actctattta
     6001 tgatagttac cgcggtgttg ttctaaatgt ccggatcacg gacggtatgg tcaaacccgg
     6061 agacaaaatc aaactgatga gtaatggcaa aacatttgat gttacagagg tcggggtctt
     6121 ctcgccaaaa gcagttgcta gagacttttt gatggtcggt gatgtcggtt atatcacagc
     6181 aagtatcaaa actgtccaag atacacgagt aggggataca gtaacgttag cagacaatcc
     6241 tgccgctgaa gctttaccag gttaccgaaa aatgaatccg atggtttatt gtggtcttta
     6301 tccaattgat acttctcgtt acaacgattt gcgggaagct ttagaaaaac tgcaattgaa
     6361 tgatgctgct ttacaattcg aaccagaaac ctctcaagca ttaggttttg ggttccgctg
     6421 tggattcctc ggacttcttc atatggatgt cgtccaagaa cgcttggaac gggaattcaa
     6481 tctagaattg atcacgactg caccatccgt tatttaccat gttaataaaa cggatggtac
     6541 gactgctaca gtagataacc cggcagattt tcctgaacct gtaacgattc aggatgtaga
     6601 agaaccattc gtcaaagcac agatcatggt cccaaatgat tttgtcggtg cagtaatgga
     6661 actttctcaa cgaaaacggg gagaattcat tacgatggat tatttagatg attaccgagt
     6721 aaatgtcgtc tataatattc cgctttctga aattgtcttt gactttttcg acaaattgaa
     6781 atcaagcacg aaaggctatg cctcattgga ttatgaaatg tctggttatc aaaaaagcaa
     6841 gttagtgaag atggacatct tgctgaatgg agaaaaagtc gatgcattaa gctttatcgt
     6901 tcaccgagac tttgcctatg aacgtggaaa agcaatcgtt gaaaaactga aaaaactaat
     6961 cccacgccaa caatttgagg tgccaatcca agcagctatt ggacaaaaaa tcgttgcccg
     7021 ttcagacatc aaagccttgc gtaaaaacgt cttggctaaa tgttatggtg gagatgtttc
     7081 tcgtaaacgt aaactcttag agaaacaaaa agaagggaag aagcggatga aacaaatcgg
     7141 ctccgtcgaa gttccgcaag aagccttcat ggccgttctg aaaatggatg agcaagatag
     7201 tgtgaaataa cttataatca atcgaaaaac ctattaaata aacctttttg agtagatgca
     7261 acactgagga ttatctgtat aaaagatttt aaactgtcat ttggttatca cggcgatact
     7321 aaaattaaca aaattggaat atttttct
//
LOCUS       DAYYHX010000139         7301 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000139 DAYYHX010000000
VERSION     DAYYHX010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.140"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..1111)
                     /locus_tag="ACHA6Y_002117"
     CDS             complement(146..1111)
                     /locus_tag="ACHA6Y_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM8988196.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="ACHA6Y_002118"
     CDS             1345..2190
                     /locus_tag="ACHA6Y_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8988197.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="ACHA6Y_002119"
     CDS             2381..3568
                     /locus_tag="ACHA6Y_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8988198.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="ACHA6Y_002120"
     CDS             3574..3930
                     /locus_tag="ACHA6Y_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM8988199.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="ACHA6Y_002121"
     CDS             3932..4270
                     /locus_tag="ACHA6Y_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM8988200.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4346..4602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4665..5699
                     /locus_tag="ACHA6Y_002122"
     CDS             4665..5699
                     /locus_tag="ACHA6Y_002122"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8988201.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="ACHA6Y_002123"
     CDS             5692..6378
                     /locus_tag="ACHA6Y_002123"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8988202.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="ACHA6Y_002124"
     CDS             6402..7250
                     /locus_tag="ACHA6Y_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8988203.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT         2417 a         1303 c         1597 g         1984 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa ttataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca t
//
LOCUS       DAYYHX010000140         7244 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000140 DAYYHX010000000
VERSION     DAYYHX010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.141"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(389..814)
                     /locus_tag="ACHA6Y_002125"
     CDS             complement(389..814)
                     /locus_tag="ACHA6Y_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988204.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1188..2159
                     /locus_tag="ACHA6Y_002126"
     CDS             1188..2159
                     /locus_tag="ACHA6Y_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8988205.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2191..3588
                     /locus_tag="ACHA6Y_002127"
     CDS             2191..3588
                     /locus_tag="ACHA6Y_002127"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8988206.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3747..4370
                     /locus_tag="ACHA6Y_002128"
     CDS             3747..4370
                     /locus_tag="ACHA6Y_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988207.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            5119..7212
                     /locus_tag="ACHA6Y_002129"
     CDS             5119..7212
                     /locus_tag="ACHA6Y_002129"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8988208.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         2465 a         1219 c         1475 g         2085 t
ORIGIN      
        1 aaatctttac tctttgattt gtaaactgaa atgatccact gtttgtttta ctgatgaagc
       61 ttgatcaaag gaagaagctg agtttgaagt tcataaaaat cagccatagt gagttttgct
      121 tggatttgtt tatcttcttc tactttttac ataattttga acaggataag attcaaatgt
      181 tttaaatgaa tctttgacta gtttgtgaca gttgtcgcat atgctcaagc ttccttggtc
      241 attaaattct gtatggatat ttttgttgtt attacatgta caataatcag gactatcgat
      301 catccatttc gtctccttta caatcatctt gcttctttac tatagcagaa aagaagggga
      361 gaaacaaaag aagatccaac agacggcact atagactgta gatgttgcca tcctcatcct
      421 tcaaacggta attaccgatt tttgtaaata tgactgttgt ttctttttta ttttcttttc
      481 ctgaaataat atattcgtca tctttggtag tgatcttgat atctgtcagc actgtttttt
      541 cttcagatga ttcagaagaa tcagttcctt gtgtctgata cgtgcgtgtc cattccattt
      601 ttttatctgt aaatgtcaac tgtcccgcgg ataaatccat atctttacgt acttttagcg
      661 tatatacact gttggtggta tctgcttttt ccgcttttaa gacagaagaa tcggttgttt
      721 cgtccgagga ttggtcagag ccgccaaaca aatcacagcc agtcaatgtc aaaagtcccg
      781 cagacaaaat aaatagtacg attaatttct tcaaaatact tcccccattt gataggataa
      841 agaaattata ccattggaat gtaaaacttc aaacggatgg aagagaaaaa cgcacaaaac
      901 acccacaaat agagtaaatt ttagaataaa attttaaaaa ggcaaaaaat ccgccacttt
      961 cagaaagcgt tttaaaaaag tgttgcaatc ttttaaaaaa atgctatatt aaaaatgaac
     1021 cggttcaaat acttgtttaa gtataattaa tagtgagagg aagttgctct actgaagact
     1081 ttagttatat ggatattgat gacaagcaac actatgcaaa gaatgcggtc atcataatcg
     1141 aacaacaaga aagagaaatc aaatgtaact ggcaggagtg agaatgtatg gcatcgatcc
     1201 gtgatgtggc taagatggct ggtgtttcag tgggaacagt ttcccgttac ttgaatggtc
     1261 agcaactgaa ggaaaagaac atgaaaaaaa tagcagaagc gatttcagcg ttggactata
     1321 aagaaaacat cattgctaaa gggttgaaga ataatcgaag cttctctatt ggacttttga
     1381 taaacgggat ttccagccgt tttgggtcgg aagttgtctc agggatcgaa agagtagctg
     1441 aagaaaatgg gtatagctta ttattaagcg ggtttgccga tcagccagaa aaaattgaac
     1501 aaaaaattga atacctgatg aagcatgtga tcgatgggct gattgtcttc ttatccgaag
     1561 aagagtggtc tggatttgaa atgctcacaa aaatgagtat tccagttatc gcattgaatt
     1621 gtccagaagg accagcaggt attgatacaa tattagtgaa tgatcgagaa agtgtagcaa
     1681 aagtgatcgc tcatcatggt gaacaaggac ataaaaaaat cggatttatt gctgccactc
     1741 aaacagacta cgtagcaaga gaacgtttag caggagccaa agaagcggtc caaaatgatc
     1801 catcgatcca attagaggtc tttacaggag actattctag aaaaagcggc tattacgggg
     1861 caaaagcatt attagaaaaa ggggcaactg ctatttttgt cagcaattac aatatgtcga
     1921 ttggtgccat cgaatgtttc aatcaagaag gtgtcagaat cgggaaggat attgcgttca
     1981 gtcactatga ttatttagat aaaaacgatc tgagtatcct tccgaaaatc acgatcacgc
     2041 caccaacaga taaaatcggg gaacgagcag cagaacagct gttaaaacga atcaaaaaag
     2101 aagaggaaga aaatgaaaaa gtgattgtga tggaaaatat catacatgga atcaactaaa
     2161 acgaactaaa aaagaaaaag aggtgctttg atgaaaaaat atcaatttcc agcaaatttt
     2221 tggtggggtt ctgcagcaag tggcccacaa acagaaggaa ggatttcagg cgatggaaaa
     2281 ggagagaaca tttttgatta ttggtacaaa aaagaaccgc aaaagttctt tgatcaagta
     2341 ggacctgaaa aaacctcgca agtttatacg aagtatcaag aggatgtaca gttgatgaaa
     2401 cagactgggc ataattcatt tcgaacatct atccaatgga gccgtttgat acctgatgaa
     2461 tctggaacaa tcaacgaaga agccgttcgt ttctatcatc gttattttga tgagttgatt
     2521 gaaaacggta tcgaaccctt tgtgaatctg tatcatttcg atatgccgat gtatctgcaa
     2581 gaaaaaggcg gatggttgaa ccgagaaacg gttgaagcct atgaaaaata tgccaaaagt
     2641 tgttttgaat tgtttggttc gaaagtaaaa aaatggttta ctcataatga accaatcgtt
     2701 ccagtcgaag caggttattt atatcgatgg cattatccag aagagtcaga tatgaaaaaa
     2761 gcgatccaag ttggctatca tgaagcgttg gcttcagcgt tagccatcaa atcttaccat
     2821 gaaatggagc aaggtggtgt tattggtatc atcttgaatc tgacaccaag ctatccaaga
     2881 gacgaatcaa accctgaaga tgttaaagca gcgcaaatag cagatgcgtt cttcaatcgt
     2941 tcattccttg atccagctgt aaaaggcgag tttccaagtg aattgattga gatagtaaaa
     3001 gaacttgatc tcatgcctgt gatccatgca gaggatttga cggttatcaa agaaaataca
     3061 gtagatcttt taggtatcaa ttattaccag ccacgccggg taaaagcaaa agaaacacca
     3121 atcgatacta aaaacggtcc aatgcctgag gattatttcg acaattatga aatgcctggt
     3181 cgtaaaatga atccttatcg cggatgggag atctatgaac aaggaatcta tgatattttg
     3241 atcaatgtac gagatcatta tggaaatatc gactgcttta tttctgaaaa cggtatgggt
     3301 gtagaaaacg aagcacgatt tatcaaagaa gatggcatga tccacgatga ttatcggatc
     3361 gaatttgtcc aatctcatct gaaatatgtc catcaagcca ttcaagaagg tgccaattgt
     3421 ttaggctatc atatgtggac atgtatggat aactggtcgt ggaccaatgc atataagaat
     3481 cgatatggct ttatttcagt agacttagca caagatggaa aaagaacaat caaaaaatcg
     3541 ggtcattggt tcaaagacgt atcagataaa aacggatttg aggcttaaga aagaatagtt
     3601 gagagcaaat gcgtgaaatt ctccattctt accattttgt gaaaaagaag ttcaattatt
     3661 tacgatcttt ttcaactttt ttgtctttct atttcatttt ttgtatataa tagaatagtc
     3721 aaaattaaat aggagtggag gaaagcatga ggaaaaaaag accgtttctt ctcttttggt
     3781 tattcggttt tttcttactg cttattttat ccgcagcatt ttcaggaaaa atgacagcag
     3841 atcttgctca gaaagaaaca gaaaaaatcg tagcaagcaa agaatcagta gataatttca
     3901 aaaaagaatt ctcaacttat tggtgggcgg agtataaaat aactgccaat tacgaaaaag
     3961 gcgaattgga catttatttg ccaaaaggaa aagaagaaaa tcaatttatt aatatcgatg
     4021 acgtacttag tgcgatttct ctcagacaat atcaatttaa agacaagcat ttgaccgttt
     4081 tgcttttgac tcatgatggc gacaaagtag cccgtttgaa aaatggaaaa gtgatcaccg
     4141 cttattctga aaaagtagac aaaaaagtgg tcgccgatcg tttagcggaa tggaaaaaga
     4201 caaatgaaca agtcaagaaa acaactgtct cttctcaaaa aaacgagtcg acaggaacaa
     4261 gtccttcaga ttctggcagt ggacaaacga taacgggaag tgaagaaaat caagcctcaa
     4321 ttattacgaa tgagtcatca caaggacaat atcaatccta tggacaatga aaaacagagg
     4381 ttgtgaaatc agcgttttga cctgagtatt agagaaacaa acggcaaaat agttcctcat
     4441 attttgccgt tttttgagct aataccgaag gtcagctgat tgaacaccgt gtatcaagag
     4501 gtcttgagcc tgccgtttaa ctctgagcca caaggagcaa tttcaaaaaa tagctcctta
     4561 tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaccaa
     4621 gaggttgtaa aattagcgtc ctgacataat ctaataccga gggtcagctg attgaacacc
     4681 gtgtatcaag aggtcttgag cctgccgttt aactctgagc cacaaggaac aatttcaaaa
     4741 aacagcttct catgttttat aaatattgtg acttgtggcc gaggagttgg caggagaaca
     4801 ccgtttaagg aagaagttgt gaaaaagctg tcctgcatca agaaataagg agctgcgaca
     4861 agaattttgt cacagcctct ttttttgtac gaataccgag aaactggctt ttgaataccg
     4921 tggagtgaag cggtcaccac aagactttta tctcttcatt agtttaatcg cttacatcgc
     4981 agcggtttta tgctgtgaaa agtggacgga ataatcttta gaaagaataa aaaaatctta
     5041 agataacaac ttttttagga aattttcatt tttcaggagc aaaataagaa aaaatgtgaa
     5101 tagagagaag tgatatacgt gacacaactt aaaaaattgt gccaaaatcg tttagcaatc
     5161 gttttgacag ctgttttctt tttctggctg aagacgatca ttgcctacta tgcagatttt
     5221 tcattaggtg tggaaggtac gatccagtac tttatcttgt ggatcaaccc aattgcgact
     5281 accttattgt tttttggcct ttctttgtat attaaaaagc caaagccagc gttagctatt
     5341 cttttgatca ttgatatttt aaatacgctt ttgttgtact tgaatattat tttttatcgg
     5401 gaattcacag atttcattac agtcaaatct gtattaggct tttcgaaagt gtcgcaagga
     5461 ttgtcaggaa gctccttttc tttgatgaag ccccatgatg tcatctactg gctggatatc
     5521 gctgtgttca ttgggctact ggtttggttg aaggtcaaga aaatacctat caaaagcaac
     5581 cctgtcggca aaccgatggc tatcgctgtt acctgtcttt cagggcttat tttttcagga
     5641 aatctagcgc tgagcgaagc caaccgtcca caattgctgc agcgaacatt tgaccgttca
     5701 tatattgtca aatatctagg aatcgatgcg tatactattt atgacggaat caaaacagga
     5761 atgactagca gtgttcgcgc gcatgcaagc agtaatggga ttgatgaagt tttagattat
     5821 acaaagaaac attatgcaga gccaaatccc gaaacatttg ggatcgctaa agggaaaaat
     5881 gtgatcgttc tccatttaga aagtttccag cagtttttga ttaatatgaa ggtagacgga
     5941 caagaagtca caccgttttt aaatagtatt ttccagaatc aagcaacgat cagctttgac
     6001 aatttctttc atgaagtagg acagggaaag acaagtgatg ctgaaaatat gttggaaact
     6061 ggcacatttg gtttacctca aggctcatta ttcacagaac ttgggtcaga caatgtcttt
     6121 caagccgcac cagcgatcct tggacaaaag caaggctata caagcgctgt tttccatggg
     6181 aatgtagcta gtttttggaa tagagatcat gtatataaaa acttaggtta tgacaatttc
     6241 tttgatcgat cttattttga tgaatccgac gaaacgttag gttatgggat attagataaa
     6301 gatttattca gagaatctgc ccaatatctt gagcatctac aacagccgtt ttacacgaaa
     6361 tttctttccg taaccaacca cacgccttat tatacggatg acaagaattt cgactttcca
     6421 tctttgaata ctggcaatag cacagtagat aattatgttc gaacggcaca ttatctggat
     6481 caatcattgg aacaattttt tacgtatttg aaaaaatctg gtatttacca aaattcaatc
     6541 tttgtgattt acggggatca ttttgggatc tcgaatacag ataataaaga tttagcaagc
     6601 gctcttggta aagatccaga tacatgggat gaatttgaca acgcacagat gcaacgagtg
     6661 ccattgatga tccatatgcc tggttatact aaagggacag tcaatcatga atacggtgga
     6721 gaaatcgatg ttttgccgac attactacat ctattgggaa tcgatgataa agattatctt
     6781 cattttggta cagacttatt atcttctcag catgatcaag tagttgcttt tcgaaatggg
     6841 aactttgtga cacccaaata caccgtccta ggaggaaaag cttacaataa tcagacagga
     6901 gaaatcatcg atacgaaagc agcaggaatc gatgaggaaa tctccaaaga tcaggaaaaa
     6961 gtcaagactg cgttaagtct ttcagataag ctgaatcagg aaaatctctt gcggttttat
     7021 gtgccaaacg gatttgagac gattgatccg aaaaaatatg actataaaaa tgaagcggaa
     7081 cgtaatgaag caatcgaaaa gcaaaaaggc gaacattcga caagtgtctt ttcaaaaaat
     7141 ggtaataaaa caacaaccaa cttatatcca gtaacaagaa cagacgttga acagcaaaaa
     7201 aataatcaat aaacaatcaa accaaccttc agtagttaga caac
//
LOCUS       DAYYHX010000141         7086 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000141 DAYYHX010000000
VERSION     DAYYHX010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.142"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..562)
                     /locus_tag="ACHA6Y_002130"
     CDS             complement(<1..562)
                     /locus_tag="ACHA6Y_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFM8988209.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(749..1399)
                     /locus_tag="ACHA6Y_002131"
     CDS             complement(749..1399)
                     /locus_tag="ACHA6Y_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988210.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1942..2877)
                     /gene="whiA"
                     /locus_tag="ACHA6Y_002132"
     CDS             complement(1942..2877)
                     /gene="whiA"
                     /locus_tag="ACHA6Y_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM8988211.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2911..3909)
                     /gene="yvcK"
                     /locus_tag="ACHA6Y_002133"
     CDS             complement(2911..3909)
                     /gene="yvcK"
                     /locus_tag="ACHA6Y_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM8988212.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3906..4790)
                     /gene="rapZ"
                     /locus_tag="ACHA6Y_002134"
     CDS             complement(3906..4790)
                     /gene="rapZ"
                     /locus_tag="ACHA6Y_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM8988213.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4925..5656)
                     /locus_tag="ACHA6Y_002135"
     CDS             complement(4925..5656)
                     /locus_tag="ACHA6Y_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM8988214.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5660..6925)
                     /locus_tag="ACHA6Y_002136"
     CDS             complement(5660..6925)
                     /locus_tag="ACHA6Y_002136"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8988215.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
BASE COUNT         2133 a         1506 c         1097 g         2350 t
ORIGIN      
        1 ctataaacat caccccaaat aaaacagaaa acagtataag cattataaaa attatcaaca
       61 accttgatcc ttcttttata gattcaggaa acagtataat tttagtacta gataaattgt
      121 ttacaccgat tagtgttaaa atatctttat tcttcttgct aacataaccc tttatgcaca
      181 aaagaacagc tatacttaaa gtaacaaata aataacgtat tccaatagaa aaatcactat
      241 aaatactctc tagcatagga ccgacaattg aagaagtcac tactgcaaat gctggaccta
      301 tccaagcaac attagaatgt tctttctctc ttttcctttg taaatctttg ttgagtatta
      361 atctatttat ttcttctgca ggcaagttaa ttttcataac cttatggggg attatccaat
      421 taaaaatcgg aaaaaggtag cctccccaaa aagtatagtg atctgtgtca atcagataat
      481 aacctgattt tgattccaga ataacatatc tcaaagccga acgaaaattt ttcatgaata
      541 tccataatct cactgtttcc acacctaatt cctccaattc caattaaatg gatttatagc
      601 atcaccaatt atgttaactg cctgaccaac ctcattgata aaattggcac cactatccca
      661 tgcttcaaca atgctatata tctatcaaac aaaaaatgga aaactaaata ctcgcgcaca
      721 taaattttac attctgtttt ttataagact aataataatc attgaaaaaa tttcttatcg
      781 gtggcgcata ttcccaattg attaatgtga tcccatattc tgcaagtatt ttacgagctt
      841 cttctaagtc atagtgagaa aaatgcataa aacctgcaat acgatcacgg actgtgagct
      901 gtggcttgct tccaccatca ttcataaaat atccagcaga gccactactt aaagtgattg
      961 ttacatcttc actttctatt attaagaatt cataatgaaa tgctttcaac tctccactat
     1021 tatttaaaag ttttatactc caacttcgtc taggttgagt tgtcacattc cctctaaaac
     1081 ttcctataaa ttctgcaatc accgtatctt caatcaaaat tctttcctca tctatttcat
     1141 attcaagact aaacggaaat tctccatatg taatctctgg caatggtggt gctgtagttc
     1201 tccaaataaa tacaataact gtgaatagaa aaaatccaac gatcgatata aatgttatga
     1261 gacctgctcg aagtaaaacc cgatataata aaaatgctgt atttcgcttt gctatatcta
     1321 gaaaaataaa aagtactgca atcataatga tagtaaaagt tgtaaaaaag cacatttttt
     1381 gccctccctt tataaacatc gattctcaaa ttctaaacca aatcttgata gttcaagttc
     1441 aggtataacg tataccaacc atacatccca attactattt atgaacttat gattatcact
     1501 gaataaatca ctccatcccc ttgttaaaac ttctacatta attacacctg gttgttttcg
     1561 actagctaat ccaatgaact tatcaaccat ccttatccac caaccacgag aatcttgacc
     1621 agaagtaaga aaaacagttc tcaaggttga tcgagcagtt ccttccagtt tttaattaat
     1681 ctccagttat agataaatac ttgatcctat ctatatcagg acttctatat taagcaaaag
     1741 tttatgtata aaactaaaat cttcattcag ttgtctcccc gctttttata cgattgtatt
     1801 aaaaaacaat agaagccttt caacattatg ttactccagt aaagttggaa ttatagtttg
     1861 acggaagaaa agcaagtata gcttttaaaa aaaactgata cattcgcatc tcctgcttca
     1921 tgtatcagtt ttttctttgg attaaactgc ttcttcacgc agtttttccg caaattcatt
     1981 tattttgcga attcggtgat taattcccga ttttgatatt gcacctgagg gaatcatttc
     2041 ccctaattct ttcaagctga cctctggatg ttgaagccga agttctgcga tttcctgaag
     2101 tttttcagga agggcatgta gaccgacctt tgcttcgatc aattcgatgt tttcgatttg
     2161 tttagaagcc gcatcaatcg tcttgttcag atttgctgtc tcacaattga ctagacgatt
     2221 gaccgaattt ctcatatccc gtacgattcg aacatcttca aatcggagca ttgagttggt
     2281 tgcaccaatc aatgttaaga aatcagcaat tttttctgct ccttttaaat acgaaatata
     2341 gccattgcgc cgttcaagcg ttcgagcatg tagaccgtaa taatttaaca tatcgcaaat
     2401 gtcttggtta tgctgttcat aaatagaaaa tatttccaaa tgataacgac tcgtttccgg
     2461 attattgact gaacctgagg ccataaaagc accacgtaag taagaacgca ttttttgcga
     2521 atttcccata atctcgtccg aaacgtgtcc attgaacatc ataccatcca tgatatcaag
     2581 atccatcaac acatcttttg tgccttgctt caaccggacg atatagacat tgttcttttt
     2641 taatttcatt tttttacgaa cgagtagctc acttcgtacc tgataatgat ctttcaaaag
     2701 agaatagata cgtctggcaa ttgcagcatt ttctgtttgg acgttcaaaa taaactgctg
     2761 gttcacaata cctaaagagc cgttcattcg cagcaaagca gctaattccg ctttcgcatg
     2821 ttctcgatga acttccaaac cagttagttc tttcttcaca tctgcagcaa atgacatcct
     2881 attctcctcc tttctccaac aataaattgc ttaatatttt gaaccgaaaa ctaaacggaa
     2941 caattcttct actactttgt ctccatcgtg gaagacacca ccatctttca attctaaaaa
     3001 gtcggtggaa atcactcgac atccttcatt tctcaatccg ttgaagtcgt gtgcgacttg
     3061 tactagatac tcatcataga tctctgggtc catataatct tcaggcactt tttccgtatt
     3121 gaccaatact gtatcgataa aaggctgatt caaatgcttg tgtaaaacac gtatatgatc
     3181 ggcatctgtg aaatgttccg tttccccttt ttgggtcatg atattacaga tatagacggt
     3241 ttcagcagtt gtcttgagca ttgcttcgcc gatttctggt atcactaaat ttggaaggat
     3301 actggtgaat aaacttccgg gtcccaaaac gaccatgtcg gcgtccataa ttgctgaaac
     3361 gacttttcga gccgctttgg cttcactttt atcatttgtg ttgcagacat acacacggtc
     3421 aatggtttta cggttgatcg cgattttaga ttctcctact gcagtcgttc cgtctttaaa
     3481 aacagcatgc aaggtcaaag gagtttctga agaaggataa acatgtccat ctacatgcat
     3541 catttttgct aataattgaa tcgcctcata agtgcttccc cgcatctcgg aaactgcggc
     3601 aatgatcaga ttaccgatcg tatgattggc taaaaactga tcttcctttt tgaaacggta
     3661 ttggaaaata tcttcataga attgtggcat atccgataac gccactaaga catttctcaa
     3721 gtcaccagga ggagccatag cgatcgattc tctgattgca ccgctgcttc caccatcatc
     3781 tgcaacagtc actactgctg tgatatctgc tccttgattt cgtaaacttt tcaggataac
     3841 tggcagacca gtgccaccgc cgaccactac gatctttggt tttcttatcc ggtaggtttt
     3901 cattttcacg accggttcac cgtctctttc cttttatcct tgtcccgatg tgtcacattg
     3961 actttatagt ttttccctaa agcatttgcc aaacgttcag tcaatgccac agaacggtgt
     4021 tgaccgcctg tacagccgat tgcaactgtc aagctgcttt ttccttcttt aatgtatcca
     4081 ggcaatacag tttccagaag atccaaaaat ttatggtaga atgattgcgt ttcatcaaag
     4141 gacatcacat aatcatagac tggctgatct tttccggtca atggtcttag ttccgggata
     4201 taatgagggt tgggcaaaaa acgcacatcc atcacaatat ccgaatcgat cggcaacccg
     4261 tatttaaaac caaatgatac aaattcgatc cgaaatcctt ggtctccctt tgatttgaat
     4321 gactcattga ttttttcacg taattgtcgt ggtgtcaatg ttgtcgtatc aatgatcacg
     4381 gaagcttggg ctttcaagtc gtctagtatc gcccgttctt tacgtatccc ttcagtgatc
     4441 aaaccgtcca tcgccattgg atgtgctcgt cttgtttctt tgtagcgagc cactagttct
     4501 tcgtctgaag catctaaaaa taagatagaa gtgtcgatga agttcgtatt ttcgatttcg
     4561 atcagcatgt tttggatttc ttcaaagaag gaacgagaac gcaaatccac tcctaaagcc
     4621 atttttgtga ctttacctga ttctttgatc aattcccaga attttggaat caatcgaggc
     4681 ggcatattat cgatacaaaa atagcccata tcttcaaaac tttggatagc tactgttttc
     4741 cctgcaccac tcatacctgt tatgatgact aattgcaaat tatctgtcat atttacgaac
     4801 ccctctcaca ttgtgcataa taaaattata tcatcccggg cgtatcatta ccagttttct
     4861 ttttctttta caaaaagatt tggaccaaat atcttttagg atatttgccc caaatccaat
     4921 ttttttactt ttccgatgct gtatccaatg cctcgcttct ttcagctaat gcacgttcga
     4981 tcgttcgatc cgctaaaata gattgagcat cgatcactgg gtaatgatta tcttcagcaa
     5041 attcatgcat caaagataat tctgtgcaac ctaggatgac tttttcacag tttaggcgct
     5101 ctaccgcttc ctctaagatt tcgtaataga gctcttgatt caaataatcc gattccttga
     5161 tctcatgata gatcaggtaa ttgatttttt cttgaagcgc agtatcagga atcactgttt
     5221 cgaaaccaag atttttgact tcgcgttcat agattccagc tttcatactt ccttctgttc
     5281 ctagaatcgc tactctccct gtcgtatgct gacggacaag ttcatttgct gcttctcttg
     5341 gcatatgcaa gataggaata tccgttgccg cttgtaactc ttcaaaaaag tagtgtgcgg
     5401 tgttacaagt caacacaatg aagtttggtc gcaataaatt ttgtttctca atatcatcca
     5461 gcagaaacgg cattggattt tcttctgatc gatctaaaat ataagctgta cgatcaggaa
     5521 ctgttgcatg attgaataag acataattca aatattcttg atcttttgta gctttcgttc
     5581 ggtggttgat caagcgaaca aaactttctg tcgccatcgt gcccattccg cctaaaatac
     5641 tgaaaaaatt ctccatgaac tagccttctt tcacatgaaa atactttttg aatcttggaa
     5701 tatagttatg gaacatatat ttcatcaaca accagcgctt gattgatcgg tctttttcat
     5761 aaaatacagt agtaccgtaa cgattttctt tgatcatttg ctcagctagt tttttgtctt
     5821 cattttcccg agcatattct aaaaagattt ttttgggcac acccatccat aatcttgctt
     5881 tatctgattg attcgttcca taggtcgttt caacaaaggg tttgttgaac actcgatctt
     5941 ctgtgacaaa acgagccaga ttcaagccgt tcaatgtcac aaagaaactg ctacgacctt
     6001 gtctcaaatt gatttcaaat aatttatatt ctccatctct aggatcatac ttcatatcga
     6061 aattggcaaa gcctgtatat tcgatttttt ccaaaaatgc cttaatggtt tgatagattt
     6121 tttcgttata atctggcatg atcacaacgt aattaccaat cgatgctggt gtaggatctt
     6181 ctaatagtgg atgaccaagg cacatcatcc gaacttgatg atcttcatct acatacgcat
     6241 ttagcacacg catattacta tcatcacccg ggataaaatc ttgtacaatc atttcacttt
     6301 tataaccggc ttcgtatatc cggcctaaaa ttaaatcgaa ctcttctcta gtctctaaaa
     6361 taaaagcttt ttttcttcct tcaaattgga cagaaagata ctccacactg tttgcaggtt
     6421 tcaaagcaac tgggaattca aaaggcaatt cctgttccaa atgtccattt actaacattt
     6481 cttctctgac gatcaatgtt ttaggatacg gaagatcata ttcttcacaa acttcataaa
     6541 agcttacttt attgatcagc ctttcaaata aagaataatc aatatatgga cagataaaat
     6601 attctgacag ctcttgttta tgttgggaga tcaattctgc atatccatca ccacaagcaa
     6661 tcaaaagata ttttttactc ttatcagaat atctttcttt tgccaaacga agcatcgtct
     6721 cgataaatac agggtcttta tcaaatcctg gaataacttc tacattgaca attttactgt
     6781 agcgggtagg ggctaactga tccgaagcat atgcttggca gattttgccg tatgcttcat
     6841 taaaagaacg tgccatacca taaacgttca tgtcgcttcc taataatata gggataaatt
     6901 cttcattttc aatactgttc atcattatct ctcacttact cataatctct tgtttttctt
     6961 ttccaactac acaaggaata tcataccata aagaaaaaag caatcaaagg aaagtcctcc
     7021 gattgctcaa ttcttttttg aacggattga acttatagga agtttttatc tataccaatc
     7081 agtgta
//
LOCUS       DAYYHX010000142         7086 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000142 DAYYHX010000000
VERSION     DAYYHX010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.143"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..374
                     /gene="rpsN"
                     /locus_tag="ACHA6Y_002137"
     CDS             105..374
                     /gene="rpsN"
                     /locus_tag="ACHA6Y_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8988216.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            389..568
                     /gene="rpmF"
                     /locus_tag="ACHA6Y_002138"
     CDS             389..568
                     /gene="rpmF"
                     /locus_tag="ACHA6Y_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8988217.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            600..977
                     /locus_tag="ACHA6Y_002139"
     CDS             600..977
                     /locus_tag="ACHA6Y_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988218.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            998..1147
                     /gene="rpmG"
                     /locus_tag="ACHA6Y_002140"
     CDS             998..1147
                     /gene="rpmG"
                     /locus_tag="ACHA6Y_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8988219.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1171..2154
                     /locus_tag="ACHA6Y_002141"
     CDS             1171..2154
                     /locus_tag="ACHA6Y_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8988220.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2184..2306
                     /locus_tag="ACHA6Y_002142"
     CDS             2184..2306
                     /locus_tag="ACHA6Y_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8988221.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2324..3847
                     /locus_tag="ACHA6Y_002143"
     CDS             2324..3847
                     /locus_tag="ACHA6Y_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8988222.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            3871..4110
                     /locus_tag="ACHA6Y_002144"
     CDS             3871..4110
                     /locus_tag="ACHA6Y_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8988223.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4303..4866)
                     /locus_tag="ACHA6Y_002145"
     CDS             complement(4303..4866)
                     /locus_tag="ACHA6Y_002145"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8988224.1"
                     /translation="MDFSISFITIYRAICNDLFDTEPLSHGNKGLIRSLHHRGKTRHT
                     KNHVESRGKICLTHSIHGHPKSADDRSDFGHWKADIVAGKTSGTCLVTLTDRKSCFLL
                     AGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITPDRGKEFSNHFAVTEVLNNMPFYFP
                     DPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(4959..5177)
                     /locus_tag="ACHA6Y_002146"
     CDS             complement(4959..5177)
                     /locus_tag="ACHA6Y_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8988225.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5366..6313
                     /locus_tag="ACHA6Y_002147"
     CDS             5366..6313
                     /locus_tag="ACHA6Y_002147"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8988226.1"
                     /translation="MYGDHGKEIAVIVNDIGEVNVDGVLVDKKGFKRTGEKLVSMPNG
                     CICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPIIQAIILAKNPLDIDLTQRL
                     EIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDIRGLLIDQFECCNILLLNN
                     SDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILNTNLFNIDETSYSMGWFKE
                     LELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKNFPGNITRAKGFLWFAQHS
                     DFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            6280..6369
                     /locus_tag="ACHA6Y_002148"
                     /pseudo
     CDS             6280..6369
                     /locus_tag="ACHA6Y_002148"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6502..6909
                     /locus_tag="ACHA6Y_002149"
     CDS             6502..6909
                     /locus_tag="ACHA6Y_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8988227.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
BASE COUNT         2508 a         1062 c         1401 g         2115 t
ORIGIN      
        1 tttattgttg acaagagcaa ctcttgttgg taaagtatca gtgtcaaaac gtaacgatta
       61 cgatttgaga tgtctaaatc aaaataaaag gagtataaag gtatatggca aaaaagtcga
      121 aaattgctaa agcaaagaaa caaatggcga tgattgaaaa gtacgcagat aaacgtcagg
      181 aattaaaagc cgcaggagat cgcaccgctc tcgctaaatt gccaagagat tcaaatccga
      241 atcgtctgag gcttcgtgat cagacggatg gtcgtccaag agggtatatg cgaaagttcg
      301 gtatgtcccg tattaagttt agagaacttg cccatcaagg gttgattcct ggagtaaaaa
      361 aagcaagttg gtaaaaggag gaaatatcat ggcagtacca gcaagaaaaa catctaaggc
      421 gaagaaacgg ttaagaagaa ctcatcaaac tgtagtaaaa ccagaaattt catttgacga
      481 agcgaatggt gattatagac gtagccatca tgtatcttta aaagggtact ataatggaaa
      541 gaaagtaatt aaaggagctt caaaatagtt taatatataa cttttaggag ggaatcaaaa
      601 tggaaattat ttacccacct ttagttgaag aaggattaaa gtattatctt gaaacgaccc
      661 agcaatcgct agataaatgc actttttatc gttcgatggt agagagagga attattactg
      721 aaacgggttt accgacccaa caggcaattg aaaatggatt agtcaaagat tactatgagg
      781 accaaggctt atcatttgat gattttttaa gaatctatcc aatttttgaa gagtatgacg
      841 aggagttatt tcaatgtatt gatggttatt gggagatacc tgttgatatg aaagaaaatt
      901 tagtttcaca attggaatct ggcgagttaa cttttgaaga tgcacaacaa attcaagcat
      961 atcttgaaga tcgataagta ttgaaggagg aagatttatg cgtcaaaata ttattttgga
     1021 atgtgtcgaa actggagaac gtctgtatct caccagcaaa aataaacgga ataaccctga
     1081 acggttagaa ttaaaaaagt attcgccaaa attgcgcaga cgggcaattt tcaaagaagt
     1141 gaaatagcta agtggtaaaa aggtgacaaa atgggcatac cagttacaat cgtttctggg
     1201 tttttagggg ctggaaaaac aacgctaatc aatcaagcct tgcaagtctc accctacccc
     1261 agggaagaaa ttattataat tgaaaatgag tttggtgaag ttgggattga tcacaaatta
     1321 ttagttcata gcgaggaacg ggtgttacaa ctaaataatg gatgtatgtg ttgcagtctt
     1381 cgcagtgatt tattagcaat attgatttct ttactggaaa caatagaaga acatcaacaa
     1441 ccaatttcac aaatcatcat tgaaactaca ggtatagcag atccccaacc aatcattcaa
     1501 acacttttaa cagcgcctca actaagagga agagtatata tcgatagttt attgactgtt
     1561 attgatagtg acagttttga acttattgaa agagaaccac aagcccttaa gcaacttgtc
     1621 atagcagatc gcatttttgt gtctagaaaa caaaacttga atttatctgc actaaaaaaa
     1681 ttacagagag aaatcaaaag tattaaccct ttatccgata ttcgaatgtt cacacatagt
     1741 gaaacgattc aaaaaaagga tttttataat ttggaaaaat tcaatcatcc cttagttttt
     1801 gaaagtgatt catcagaaaa tcactataat ggcaaacact atcatcaaca tcatcaagaa
     1861 catgagttta aatcaattct cttaacttca gatcaagaca taaaaaaaga ttttttattc
     1921 caattaattg accggctgat tttaaccaac caagggagcc tgtatcgttt taaaggattg
     1981 attgctttgc aagatcaaga ttttatagtt gccatacaag gggtaaacga acaggttaat
     2041 tttcaataca ccactcgaca aaaagaccaa accactatta ttctaattgg aaaaatgttg
     2101 gatgaagaaa ggatcaaaca gagttttgat aaattactgg cggaaagcaa ataatgataa
     2161 gtattgatta ggggtaattt ctaatggaga aaacggatat atctagtgca tatcgacgac
     2221 ttaaaagccc gaatatcaaa actagaaaaa gggcattaaa aattatcaaa gaacataaac
     2281 aaaataaaat gaagaaatta gcataattaa ggaaggaaaa agaatgaaga aaatcagtgt
     2341 aggacttatt gggatagcag ctctaggatt attgggagct tgctccagca ctaatgatgc
     2401 aaaagtatcg aatgataaag atggaaaatt agaaattgta acaacattct atccaatgta
     2461 tgatttcacc aaaaacatcg tcggcgatga agcgaacgtg gatttaatgg ttccagcggg
     2521 atcagaacct catgactatg agccctcagc taaggatatg gcaaaagctc atgatgctga
     2581 tgtttttgta tatcataacg aaaacatgga atcatgggta ccaaaagcta aagaaagttg
     2641 gaagaaagct gggcctaatg tagtagaagg aacaaaagac atgattttat tgccaggtag
     2701 tgaagaagaa gatcatgatc acggagaaga agatcaccat catgaactag atcctcatac
     2761 gtgggtttca cccaaaatgg caattaagga agtttcaaat atcaaggatc agttagttaa
     2821 attgtatccg aaaaaagcaa aagtatttga gacaaatgct gaaaagtatt tgactaaact
     2881 gaaacggttg gatgctgact ataccactag tcttaaagaa gcgaaacaaa aaagttttgt
     2941 gacacaacat gcagcctttg gatatttagc tttggattat ggattgatac aagttcctat
     3001 tgctggacta agtcctgaag aagagccatc ttctggtcga ttagctgaat taaaagagta
     3061 tgttaaaaaa aataagatca attatattta ctttgaaaag aatgctaatg ataaaatcgc
     3121 aaaaactttg gccaatgaag ctgggataaa attggaagta ttgaatcctc ttgaaagtct
     3181 tacaaaagaa caaatggata atggtgaaga ttatgtttca gtaatggaag acaatctaaa
     3241 agcgttagag aaaacaacta tggttgctgg tgaagaggta gttccagaaa aagaagcgaa
     3301 ggatgaaaag acagttgcta atgggtactt taaagatgcc gatgtgaagg atcgcgaatt
     3361 gagtgattat acaggcgaat ggcaatctgt ttatccgttg ttaaaagatg gaattttgga
     3421 tgaagttttc gattacaaag caaaactcaa taaagacatg acagctgccg agtacaaaga
     3481 ttattatact actggctata agacagatat tgatactatc aacattaaag acaacaccat
     3541 tgattttgta gtcaacggtg aacatcatca atacacatac aaatatgttg gatacaaaat
     3601 tttgaattat gaaaaaggaa accgtggtgt tcgttttaat tttgaaactg atgatgcagg
     3661 tgctggtcgt tttaaatata ttcaatttag cgatcatgga attgcaccaa gtaaggcaga
     3721 gcatttccat attttctttg gtggagaaag tcaagaaaaa ctttataatg aaatgcataa
     3781 ttggcctact ttctatccag cttcattatc tgagcatgag attgctcaag aaatgatggc
     3841 ccattaaaat ccaatctttg gaggaaatac atggtaagaa aagtattatt gtctatggat
     3901 tccaagaata ttgatgctta tcggcgttat caatttgcaa agcaaatgag attaaatggg
     3961 agtacattag ctgatattgc tgcaacttta catgttagta ttgacacagt tcatagagta
     4021 ttaaaacggg atcctgattg ttgttttatc aaggtcaata ctggaagaag tagacgttta
     4081 gcaaaacggc ataatagctt ggccaattaa ttgataatac cagagtagat catttttccc
     4141 ttatttggat ttgatctgct ttttattatt tcttgaattg tcaaatcaag cgcaacactt
     4201 tatcaaagaa gacttcataa ggtgttctcc aatctaaaca ctttctaggt ctgttattca
     4261 gtttttcaat catctgattt atcgttgagt tatctaccgt agctatctct ttcgattttg
     4321 gcaagtattc tcgaatgagt ctattcgtat tttaatttgt tcattgttgc caagggcatg
     4381 aggatctgga aaataaaagg gcatattatt caatacttct gtaactgcga aatgattgga
     4441 gaattctttt cctctatctg gagtgatcgt tttgaccgcc tcatttggaa tactgcttaa
     4501 aagttcaatc attttctcgc tcacataagc ggctttcttt ctggggacct tctttcctgc
     4561 taaaaggaag catgattttc tgtctgtcaa agtcactaaa caagtaccac tagttttgcc
     4621 agctacaata tcggctttcc aatgaccaaa atcacttcta tcatctgcgg attttggatg
     4681 gccatggatc gaatgagtaa ggcaaatctt cccacgagac tcgacatgat tttttgtatg
     4741 tcttgttttt cctcgatggt gcaatgaacg gatcaggcct ttattgccgt gtgagagggg
     4801 ttctgtatcg aaaagatcat tgcaaattgc acggtagatc gtgataaaac taattgaaaa
     4861 gtccatatgt tcatgcttca aacgattcga gattttctct ggcgaccact gaaaatcgac
     4921 aaataaatga cgaacaattg accaaactca cggatcattt agaatgtgat aacgtccgca
     4981 tttttttctt cgataagcat aatttttttc cgctttggaa ggtgattagt ttttttcttg
     5041 tttcttctca gttcatgtaa aatagtaaac gggctatgct gaatgtcgtt gccaattata
     5101 ctaatagaaa atccttcttc gtgaagcaga aagattcttt ctcgtttgag tatagtaaga
     5161 tgacaatact ggctcataat tattttcctt ctaggagtgt tctcgacaaa cttatactag
     5221 ttggatatcg attatgactc atttttatgt gttgcactta aagtgtaaat tcaaggtata
     5281 gaaagaggtt ccctatgaaa aaatacctat tacaatctta actggctatc ttggttcagg
     5341 aaaaacgata cttctaaatc atattctgta cggtgatcac ggaaaggaaa ttgctgtcat
     5401 tgtaaatgac ataggggaag taaatgttga tggggtatta gtcgacaaga aaggttttaa
     5461 aagaactgga gaaaaacttg tttcgatgcc gaatggatgc atttgttgta cattacgcga
     5521 agatttgatt gttgaactag atcaattagt gcaattttct gacttgaatc aaattgttat
     5581 tgaagcttca ggaatcagca aaccaattcc aattattcaa gcaattatat tagctaaaaa
     5641 ccctttagac attgatttga cccaacgatt agagattggt gccattatta tagtagtgga
     5701 tgtaaaacgt ttttgggata atttcgaaac gggtgaaaag attcaggaaa gaaaagctga
     5761 aggtaatgta gttgaagaag aacaggatat tcgtggcttg ttaattgatc aattcgagtg
     5821 ttgtaacata ttacttttaa ataatagtga cttagtgtca accaagacga tttacaaaat
     5881 taacggtcta attgctaagt tacagtccaa tgccaaaata attaggacaa ttaaaggtga
     5941 agtagactta tcgaaaattc taaatacaaa tctttttaat atagatgaga cttcctattc
     6001 aatgggctgg ttcaaagagt tagagcttgg ctatcaaaat catattcctg aaacagaaga
     6061 atatggtatc tcaagtttta tctatcgaaa tcggttactc cttgacgcac aaaaatttgc
     6121 ccatcttacg gataaaaatt ttcctggcaa tattacaaga gcaaaagggt ttctttggtt
     6181 tgcccaacat agtgattttt cgacgatctt tgaaacatcg ggtagccata aaagtattga
     6241 atcattaaat tattgggtag ctagtttacc taaaattgag tgtcaaatga ttatacaaaa
     6301 aaacccgaat tgaaggaagc ctggcatccg ttgtttggtg aacgcattaa taaactagtt
     6361 tttattggta tgaagatgga tcaacaatgt attaaggaac aacttgaggg ttgtttaact
     6421 gattctctta atgcagataa tttccgaaaa atttcaccat ttccatggaa aaattctaat
     6481 atgtttagcc agatatcgtt aatgaaattt ttaagaaaag ctggtgtcaa gataggcgat
     6541 gtcataatta ttggcatttt gattattccc tcttttattc ccttatttgt attctctata
     6601 gaaaaggaga atgaaacaac tgaagatgct atcaaatatg ccatcgttca aattgatggc
     6661 aaggaaatgg atcgcttcga tttggataag atcaatcata aactcgtgaa ttatcatcct
     6721 gcgaagggac aatataatat tgttgagatt aaagatggaa gaattcgagt taaagaagat
     6781 aatagtcctg atcaaatcgc ggttagaact ggatggatct caaaacccgg tcaaacgagt
     6841 atttgtttac cccataagtt agttattagt attgagaaaa aagaaagcaa agattactac
     6901 atttactaat ctggaaatag atgaggaggt ttttaataag aaaaagatat tattattaat
     6961 aatagcaggg gctttattaa gtgtgttaag cgcttgtgga caagaaaaag aagcggtgac
     7021 aacaggcaca agtatcttta acgggacctg caatggttta gttaacagtc ggttagatta
     7081 cttatt
//
LOCUS       DAYYHX010000143         7080 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000143 DAYYHX010000000
VERSION     DAYYHX010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.144"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..872)
                     /locus_tag="ACHA6Y_002150"
     CDS             complement(<1..872)
                     /locus_tag="ACHA6Y_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8988228.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKL"
     gene            complement(884..1837)
                     /locus_tag="ACHA6Y_002151"
     CDS             complement(884..1837)
                     /locus_tag="ACHA6Y_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8988229.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(1827..2897)
                     /locus_tag="ACHA6Y_002152"
     CDS             complement(1827..2897)
                     /locus_tag="ACHA6Y_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8988230.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(2903..3946)
                     /locus_tag="ACHA6Y_002153"
     CDS             complement(2903..3946)
                     /locus_tag="ACHA6Y_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8988231.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(3947..4888)
                     /gene="opp3b"
                     /locus_tag="ACHA6Y_002154"
     CDS             complement(3947..4888)
                     /gene="opp3b"
                     /locus_tag="ACHA6Y_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8988232.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            4972..5319
                     /locus_tag="ACHA6Y_002155"
     CDS             4972..5319
                     /locus_tag="ACHA6Y_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM8988233.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(5389..7050)
                     /locus_tag="ACHA6Y_002156"
     CDS             complement(5389..7050)
                     /locus_tag="ACHA6Y_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8988234.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         1960 a         1489 c         1309 g         2322 t
ORIGIN      
        1 attaatttat gccagtcgtg aatagaaaaa tcagcatgtg cctcttcttt tggaaaagat
       61 tctacaaata agtagtcata ttgtttgacc aatttcccag ataattgttc aagataatct
      121 tctcgctggt tcttttgttt cagataaaga tccattactt ttctcttttg tttctgataa
      181 ttgcttgcct gatctaacct gatctttcta tggtgagcgg agcggacttt cacattcaat
      241 ttccgctgtg catgttgcag cttttcttct gtctggtgaa attttggtaa agttacttct
      301 agaggtcgag atgtttggat caatgtcttc ggatgatagg cgatcccgat ccatttagct
      361 aaagattctt tttttactgg cgaaacatca caaagtagtg cgacataaaa ttcttcatta
      421 tatcgagcag aaatcgtagc ggagcgaatc gatccttcaa ctggccgatg ctgatttatc
      481 ttgattagtt ctttttgttt tggtaacttt aagtagccat tttccagata gatcgtacgg
      541 gtttggttgt tggtcgtata agattgccac atactttttt tcgttttaag ctttgggaag
      601 ctggcacgtc ccttgaaata attgcgaaaa gcccgatcca aattaagctg tgcatttgct
      661 aaagcaagac tgtctacttc ttttaagaat gggtattctt tttttaatgt agcgggtgtg
      721 agcgaaaaat tggctactcc tgtttgttgc caacttttct gccgcttttt cagcaaatgg
      781 ttataggtaa atcgagcaca tccaaaagta cgaatcaatt gctgtttttg ttcttctgtc
      841 ggatacatac ggaatttaaa agcttttagc atctatgtcc ctcctagtta gcaaccagaa
      901 gagattcctt ttgtcgtttt agtttttttg cgtaatatgg gacttcctgc tcagttgcat
      961 agacaaaatg ttctggtgcg atttcacgaa gttgacgtac ttgtccgtca tctggttcag
     1021 gttgatattt gattcttctg cgttttctct catggtctgg atctggtaga ggaatagcag
     1081 ataacaaact ttctgtataa ggatgaacac cgtgattata gatttcatca cttgttccca
     1141 tctctaataa caagccgtta tgcattacac cgattcggtc actaatatgt ttaaccatcg
     1201 acaaatcatg tgcaataaat agataggtca attgatgttc tttttggaga tcttgcaaca
     1261 aattgaccac ttgggcttgg atcgacacat ctagtgcaga aattggttca tcacaaatga
     1321 taaaacgcgg ttttactgcc aatgctcgtg caataccaat acgctggcgt tgcccacctg
     1381 aaaattcatg aggataacga gtaccgtggc ttggattcaa tccaacagtt ctcaatagat
     1441 catcgacttt ttttgcacgc tcttcagatg atttagctag tccattaaca tcaattcctt
     1501 cggcaatgat atctctgact ttcatacgag gattcaacga agcgtatgga tcttgaaaga
     1561 tcatctgaac atcatgccgg aatttcgtca aacctttttt atctttgatc ttcgttatat
     1621 cttgaccgtc gaataatatc tcaccatcag taggttcatt caagcgaatg atcgtacggc
     1681 cagtagtgga ttttccgctt ccagattctc caactaaacc gaatgtttct ccttcataaa
     1741 tgtgaaagct gatgtcattt acagcatgta cttcattctt ttttcctaca ttgaaatact
     1801 gtttcaatcc tttaacttca agtagtttat ttggcatcag caattcctcc ttgaccgtaa
     1861 gtatgttttt gtttctcttt aaagatagcc cagcgacgtt ggatttcttc tggcggtgtc
     1921 actttgggtg cctgcggagc aagtagccaa gttgctgcct tgtgtgtttt tgatagttca
     1981 aaaaatggtg gttcttgttc tgcatcgatt ttcatcgcga attcatttct tggatagaat
     2041 gcgtctcctt ttggcgggtt caataaatcc ggtggagaac caggaatagc atacaatcga
     2101 tcgttcgcac tatctaatgt cggcattgaa ccaagcagtc cccaagtata gggatgttgc
     2161 gggttgtaga aaatttcttc cgatgttcca acttcaacga ttcttcctcc atacatgaca
     2221 gcgacacgat cagcgacatt cgctactaca ccaaggtcat gagtgataaa gataattgat
     2281 gtatcgattt ttgtctgaat ttctttcagc agctccaaaa tctgtgcttg aatagtcaca
     2341 tcaagcgctg ttgtaggttc atcagcaatc aggatttccg gataacatac cagtgcaatc
     2401 gcgatcacga tccgttgacg ctgacctcct gagaactgat gcggataact gttgatacgt
     2461 ttctcagctt cgggtattcc aactaattta agcaattcta atgcagcttt ttgtccctct
     2521 ttttttgaaa ctttattatg ctttctaagg gactcagcga cttgcttccc aatgggcatc
     2581 gtcggatcta aagaggtcat tggatcttgg aaaatcatcg ctatttcttt accacggatt
     2641 ttttgcatct ctctttctga ttttccaacg atatcgttac ctttaaagag gatttgacca
     2701 gcatctatat tggcgttgct tgacagcaag cgcatgatgc ttctggtcgt cactgatttt
     2761 ccacttccag attccccaac gatcgccaat gtttctcctt tattcaaatg aaaattgaca
     2821 ccgcgaatcg ctcgtacctt tccagcatac gtatcaaaag agatttctaa gtctttcact
     2881 tctaaaattt tcgtcatcgt cttcactctt tcattttagg atcaaaggca tcacgcaatc
     2941 catcagcaag taagttaaag ccaatcatga taaccgataa agttactgcc ggaatccata
     3001 atagataagg cagatacagg aatgtcttgt acccttcgct caacatcgtt cctaatgaag
     3061 ctgacggagg cgtaagacct aaaccgataa agcttaaaaa ggcttcgaag aaaatagcag
     3121 atggaatact aaacatcatt tgaataataa tgacactaga gatattcggc aaaatatgtt
     3181 tgaatgcaat tttcattctt gattcaccta atgtcaatcc agctaaaaca tattcttgat
     3241 cttttaattt cattgtttgt gcacgaacga tacgtgccat tggtatccag tttgtgatgg
     3301 ccatagcagc cacgattgaa aaaattcctg gttcaaaaac gaccaacatc aaaatcatga
     3361 caacgaggtt tggaattcct gataagattt ccaaaaaacg ttgcatcacg ttatcgacac
     3421 gtccgcctaa caaaccagaa accaacccgt aagctacacc aatcgttaca tctagtaatg
     3481 cagcaataaa tgcgatcagc aacgagattc tcattcccat aaataagcgg cttaatacat
     3541 cacgtcccaa accgtctgtt cctaaaaaga aatttacgtt tgaagggacg tttgcttgag
     3601 catatttatc aacaagttca ccaccaacca ttgcttttcc attcaatcca tcgatattga
     3661 ttcccggaat acgaggaggc aaattgatat aagccacgtt ttgggcagtt ggatcatgag
     3721 gagaaacaaa gattgtgatc acagagatca aaatgatgac caatagtaaa accattgaaa
     3781 taacggcagc tttgtttttc tttaaacggc gccatgaatc ttggataaaa ttcaaagaag
     3841 gtgcagtaat ctgttcacgt tcttcttcgg tatttttttg taatggaaga aattcatccg
     3901 cagggatatc tgcaactgtt tcataacgct ttggtatcat ttccatttat cctcggctcc
     3961 cttctgaaac acgaattctt ggatcaacta gaccgtacaa tagatccacg atcaaaataa
     4021 caaaaatcag cataaatgag taaaggatcg ttacagccat gatcgtagga taatcatttg
     4081 ttgtgattga cttaacgaat tgttccccga taccaggaat agcaaagata ttttctacca
     4141 ccagtgatcc ggtcatcaat gctacagcta acggtcctaa taaagtcatc aatggaatca
     4201 aactatttcg aagaccatgt ttaaaggcga tttcccaacg gctcaatcct tttgcttttg
     4261 ctaattccac ataatcacta tgcagtactt ccaccatttc cgtacggata aaccgagcag
     4321 agtcagcaag cggcgacatg gccaacgcaa ttgttggtaa gattgtgtac acaaagccat
     4381 cccattttgc gattggcaaa acttgtaatt tgattgcaaa gatatattgt aataaaacag
     4441 caaatacaaa gtttggaatg gaacgaccta atatcgctac gagtgtagaa gatgtatctg
     4501 cccatgtatt ttgcttcatg gcagagatcg ttcctaaaat cgtccctagg actgttccaa
     4561 agataattgc ctgtaaccct aactgcaagg atggtccgat tcttccagct aacaaatgtg
     4621 cgactggctg gtttttaaat tggaaggaaa ttccaaaatc tccttgaagc aaattagaaa
     4681 gataaatccc atattgtacg ataactggtt tatccaaacc gacttgttta tttagcatct
     4741 cgattgtttc aggactcaaa cgttcctgat tcgtatatgg cgtgcccggc agcaattgca
     4801 tcaagaaaaa ggtgattgtt gcaattaacc aaagcgtgat gatcatgaaa aagacacgtt
     4861 ttaatacata tttgatataa ctattcacta atgtttcccc ctagcttttg acttctgaca
     4921 gatgtttcgc tacattatta gataatgaac caaaaataaa ggagcaatca aatgaaaaaa
     4981 aaatctatcc cctacgcagt agcttttcta ctgattctgg ttattttaat aaaaaatctc
     5041 atcaaccatt cctttacact cattcaatta tctaatgatc tattcttatg gtcattgccg
     5101 tttctgatta tcggcggttt tttatgggtc ttttcttcgg gtttcttcga tcattttcag
     5161 cgatcggtcc atcttgcacg aacaaggaac cgaaagaaaa aaccagaatt ttccacgctc
     5221 tcgtctgcca gctacggtat gtacagcttt tggctgatca ttgccggaat actaattgct
     5281 ctttctgcta ttttcatgct tttttctttg ttaggataag tgaatatgag aacgaggttg
     5341 cgacatttgc cgcagcctcg ttttttctca tgctcacggt attaacgatt attcttcaac
     5401 gtatgtccat ttgtaatccc atgaagcgcc tgccgcatgg tgaacgatac ctttgatttt
     5461 ttcattcact aggtgacctt ctgctttttg gtacaccgga acgacaccca tatcatcaga
     5521 gatgatcttg attgcttttt ggaaatcatc ccaacgggct tgttcatcgc cagcgtctgt
     5581 tgatgaagct gctttaactg cttcgtcata ttctgcattg ctccagcgac cacggttgta
     5641 tgaattacct gtgacaaaca aatcagtaaa gctgcttggg tctgcatagt cagcagccca
     5701 tccgcctagt actgcgtcaa aatcacccga agttgagcgg tcaagacgta cagagaatgg
     5761 aacaggtgtt ggtttgactg ttacgccatc caagttttct tggattgagt tttgaacata
     5821 ttcgattact tttttcgatg aatcatcatc agaagccatc aaatcaaatt ctaaagaatc
     5881 aacgcccaat tcttttttcg ctttttccca atattctttt gctttatctt tgtcataaga
     5941 aactaatttt ccagcttctt ttgcaaagtc ttcgttcgtt gttggacttt ttaccatatc
     6001 tgatgggatc aatcctgtag agacaactga tccgtcacct agtacttgtt ttactaaagc
     6061 ttcacgatcg atagaataag aaatagcttt tcttaggttc acattgttga atggtgaatt
     6121 cttttcacgt tgattgaatt cgatatagct tgtccgcgct tctttaacag aagtataagc
     6181 tggatcgttt gcgttttgct gtgctaactc accagacaag atcacgtcat ctgcttggcc
     6241 atctttgaat aagttcaatg cagtagaaga ttcttttact acactgactt tgatttcaga
     6301 aagttttact gtatctttgt cccagtaatg atcatttttc ttataagtcc attctgtatc
     6361 tgttcctggt ccatcaaaat cttctaatgt gaatggtcca ttataaacag ctttatcact
     6421 tgcagatgca taggcatccc cattttcttc cactacggat tgtttttgtg ggaaaaatga
     6481 tgggaaagca agcagatatt ggaaatatgg tgtttgtttc gtcaatttga tttctaattc
     6541 atagtcacca actgctttga tccctagttc tgatttatct ttctttccag ctgtaatgtc
     6601 ttctgcattt tcaactggcg catacaggta agcatattca gaagctgtag ctgggtctac
     6661 tgttcgttgc cagccataaa cgtaatccgc tgctgtaaca ggatcaccat ttgaccattt
     6721 tgcgtcttct cgtaatttga ttttgtaagt taatccgtct tcactgactt ctgccaactc
     6781 gctagctcct gccggctgtg gtttgctgtc ttcatcaaga cggtaaattc cttcataaac
     6841 gttattcaat gctgtaaaac tgattgtatc tgttgcaact gataagtcag ctgtaggcat
     6901 ttcttgaggc acgactaagt taaatacgcc tcctccatct gctgaaccgc tttttgctga
     6961 actactgctt gtggctgaac tgccgccata acatccagca agtacaaggc cgcataccgc
     7021 aaccaaacca aatgtaaatt tctttttcat tttacgtacc cctttattgg aattttcaga
//
LOCUS       DAYYHX010000144         7058 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000144 DAYYHX010000000
VERSION     DAYYHX010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.145"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2668)
                     /locus_tag="ACHA6Y_002157"
     CDS             complement(<1..2668)
                     /locus_tag="ACHA6Y_002157"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8988235.1"
                     /translation="MNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFICY
                     LLAVSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAF
                     FYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFW
                     YALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVSPVLLIVGGLIL
                     LLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLF
                     KVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQ
                     TRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGG
                     TANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRR
                     KQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQL
                     STMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKID
                     KPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNF
                     GAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEK
                     NLTEKQKEILEDYRLIQYDLVAGNQYSATWATQ"
     gene            complement(2655..3017)
                     /locus_tag="ACHA6Y_002158"
     CDS             complement(2655..3017)
                     /locus_tag="ACHA6Y_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988236.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFLRTSWQLEYS"
     gene            3236..4456
                     /gene="sstT"
                     /locus_tag="ACHA6Y_002159"
     CDS             3236..4456
                     /gene="sstT"
                     /locus_tag="ACHA6Y_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM8988237.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4603..5238
                     /locus_tag="ACHA6Y_002160"
     CDS             4603..5238
                     /locus_tag="ACHA6Y_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8988238.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            5429..6169
                     /gene="truA"
                     /locus_tag="ACHA6Y_002161"
     CDS             5429..6169
                     /gene="truA"
                     /locus_tag="ACHA6Y_002161"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8988239.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6314..>7058)
                     /locus_tag="ACHA6Y_002162"
     CDS             complement(6314..>7058)
                     /locus_tag="ACHA6Y_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988240.1"
                     /translation="DLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSL
                     FIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAV
                     AIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNT
                     VLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYL
                     SIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         2270 a         1103 c         1350 g         2335 t
ORIGIN      
        1 tttgtgtcgc ccatgttgca ctgtactggt tacctgcaac aagatcatat tgtatcagac
       61 gataatcttc tagaatttct ttttgtttct cagttaaatt tttttcttgg ataatttgac
      121 tatcctctgt aacaaagacc tgttttccat taaaacgatt agacaagctt tctgtaggat
      181 caattgttaa tgcaggtaga tgattagata cttctgtcaa caacgcatag taaggagtta
      241 ctttgatatt cgcatgctca agagccatag caccaaagtt attaggagca acaacttctt
      301 tatttaacgt agctgcttgt tcacgactat acttgttgtt ataaatgaaa taatctgtct
      361 ggtgctgttc taatccatat tttttcgaat cattcccact atagattgcg ggtaaatggt
      421 caccataaaa aacaaaggta atcggtttat cgattttgtc gatttcttgg atgaattttt
      481 ttactgctgt atcggtatag tgaaggcctt gcatataagt ttgtaattct tttcttctat
      541 taggaatgac agcagttcct tcagcagtgt agttcaattc actatagtaa tcatcataag
      601 gcatatggtt ttgcatagtg gataattgga tgaattgtga cgtttctgta ttgctttgta
      661 aagctttcaa ggtttctttg tatgctgaat catccgatat gcgtgggctt tgttctattt
      721 tatccgtata agttaattta tccggactgt ctaaataata gaacctgtca aaaccaaatt
      781 tatcaaacac ttgttttctg cgataaagag cagctgtaaa aggatggatg gctatttttt
      841 catcgaatag atcggtaata tttggactta ttttctgacg ctccactagc tgggtataag
      901 gtgtaactaa cgaagcagac aggttactga tactcaagcc tgtcagtcct tcccattcca
      961 tgttagctgt tccgccacca taaccgacgg ataacatcaa tcctcctgtt ccttctgtta
     1021 taatgctttt gatgtaagga atagggtctg taggtactgt cagattaggg acacgtgcag
     1081 gatcactgaa gctttcactt aaattgagaa cgatcgtctg attgtttaac cagtcacttc
     1141 ttgtttggtt gatttcatct gcctttgtgt tgtacttttt cattattcca ttaatttttt
     1201 ctttcgaata gccagcaggt tcttccatga ttttaatgtc gatattgttt aggaattgaa
     1261 tgactggtcc gttatctttt acggcgcctt gctggttaaa gaaagtcttg tttactttaa
     1321 ataaattaaa cagcaagaaa gaaggcgaat ttttgtgatt aatgaaaaag acacccgaaa
     1381 aacatcctaa taatacagcg atactgataa atcgacgttt ccaatgaatc ttcaaggaat
     1441 actgtttttg tagtctacgt tgtacaataa tactagtaat caataatagg agaaggatca
     1501 atccgcccac gattaataag actgggctaa ccatcgataa tagttctgac aatccggtca
     1561 gtatttttaa atctgaagga aagacaggct cttcccgcat ttccattttg attactgtcg
     1621 aaatagtgag cagtaagtta atgatcaagg tagcagtcaa ggcgtaccaa aaacgattgg
     1681 aaatcaacaa tagtaagaag aaaaatccca taataatgat gattgtcagg atcatgggtg
     1741 cttgtcgctg agtaaatatt tttaaggcaa cctggatagt ttctttccaa ccttcttttg
     1801 gttctaaaac gaaaatctgc acgaaggtaa agcagtaaaa aaatgctgca actaaaaaat
     1861 aacttttatg aacaaaaagc caatgaattc tttttttcca acgctgagtt aattgttcaa
     1921 tcgaatcaaa agcgaagtat tcgatgaaac gatgaatgaa acggattttt tgtatataaa
     1981 ataaaaagat aactaggagg gacactgcta ataaatagca aataaaaaac tgcccgatat
     2041 ttatcagcca tgccttccct gagtttggaa attcacgttt cataaatgta gatacgcgat
     2101 aagaaactaa agggaaatta catataactt gagtgacaat caaaaataca gcaagaaaat
     2161 ttcctcttcc ttttatacct aatttatcag atagcatttc taaaaataaa aacgaagaga
     2221 aagtataata cattgcgaag agagaatatg gtttagaaaa cctcagcgct gtatctgtat
     2281 ttcctctgat gatgagtgaa atttgtgtca tcaaaataat caaaccgaat aaaacaatca
     2341 agctgataaa tacttgagga agcttatagc ggaccttttc tttccagttc cattctttca
     2401 aaccataacc cattaaaaat aaataaaaaa tccataaaat actctgtcca ttctgtaatg
     2461 caaacgggtc tttattaaat agagtagagc agccaactaa tccaattgtt aaaaataata
     2521 gtagatattt aattgttgtt ttcgataatt tatttaaaaa tccaactgta taaggaatac
     2581 tacaaaaaag caaagtacaa gaaacagcat aagtaaagta attctgactg ataggtgtta
     2641 ggataagcca ataatcacga atattcaagc tgccagcttg tacgtaaaaa aaagatattg
     2701 caaactagtg tggctattag gaatataaag gcaaaacgac ttaaataatg taaaactttt
     2761 aaacgggcga ttttttgata ttggtaaccc atgtaaagtg ggatcaaatt tgcaccaata
     2821 acaacggaag tttgtaaaaa tgtatagatg tagaatcgac cagggtttgt tgcatatgta
     2881 ggaatagtga ctgctgaaat caatatacgc tgtaaaacca tcattataca agcgacaaag
     2941 attgtcagga agccataact ctccttcttt atttttttca agaataaaca cctcacaaaa
     3001 aaattggaca aaaacataag aaaacgtttc ctgataagtt gcccattaat aacatcctaa
     3061 cttagaatat ctcaaaaaac aacgtgtaga cagaaaaagt acaaaattag caagaaatgg
     3121 ttgtttgtat tgttagaatt tttagaaaat tataccttta gtttctttgc ataaaatgat
     3181 acaatgattg ttaaaattgt gaaagggtgg tcatttgtta gagaagtttt tggatatgtc
     3241 gttagttcaa cgtattgttg ttggtatttt agtcggaata ttgtttggta tctttttccc
     3301 cacatggaca tttatttcaa tattgggaac gttgtttgtc agttgcttga aggcaatcgc
     3361 acctattctg gtttttttct tgacgatggc gtcaattgcc aagcataaaa ttgggaataa
     3421 gacattcgtg aagcctattt tgatcttata tttagtggga acattgcttt ctgcacttgt
     3481 agcagttatt gtaagttcaa tttttactgt tcctattact ttacaagaaa cagtatctga
     3541 aaaagcaccg caaagtttgg aaagtgtttt atctactatg ttgacgaatg tcacacaaaa
     3601 tcctgttcaa tctcttattg atgcgaatta ccttggcgtc ttattctggg ctgtattgtt
     3661 gggactagct tttcgagcac gttcagaatc gacaaaagag ttggtggatc agatatctat
     3721 agcgttgtca caagtcgtac aagtgattat tgcatttgct ccgataggga ttttaggatt
     3781 ggtttatcaa tcaattgcta cgactggaat agctggattg gcagagtatc tgcagctttt
     3841 acttgttttg atcggtacga tgctgtttgt cgcattagtt gtgtacccat tcctcacgtt
     3901 tttgtttatt aaagaaaatc cctatccatt gattttcttt tgtttgaaag aaagcgcact
     3961 tcccgctttc tttacccgaa gttctgccgc aaatatcccg ataaacatga tgttggctga
     4021 aagattgaaa ttgacaaaag agtcttattc catatcgatc ccgttaggtg ctacgatcaa
     4081 tatgggcggt gccgcgatta ccatttcctt gatgacgttg actgctgtac attctttagg
     4141 gattgaagca ccttttgttt tgaaagtcct tttatgtatt ttgtctgctt tagctgcttg
     4201 tggagcttca ggtgttgcgg gcggttcact gttattgatt ccattagcat gtagtttgtt
     4261 tggtatctct aatgatgtcg cgatgcaaat cgtcggtatt ggatttatta tcggtgttat
     4321 ccaggattca atggaaactg ccttaaattc gtcaagtgat ctgttgttta ctgcaatagg
     4381 agagctaggc gcgcgtaaaa gaagtgggga aaaaatcaaa ttgaaagatt gtttagatgg
     4441 attaagtgaa cgttgaacgt tagatattta gtatttttaa aatgattcga gtttttaatg
     4501 agttatatag atgttcgtgt aaatagaggt ttctgttaaa tcaagataaa gtcgcttata
     4561 ataacaatta aaagcatagg taattgttat tggaggcgta tcatggattt tttacgtaaa
     4621 tttggttttt tctttttatt attaggcgtg ctgagtgatt ttttgactcc gtacgtatta
     4681 ggattgtatt atcctgaatt agatcaaatg aagagcgtga tcagtgtatt tggagaggtt
     4741 tccagtcctg taagaaaagc gtttcttatt tggtctgtgg tttcagggat ctttttcatc
     4801 ctgtcattgc ctgcattgta tgctgcattt gtaagtacat ctcgtgtgtt agctacccta
     4861 gttacagcag ccatcgcgtt ctatggtata ggtgattgta tctttacagg tttgttcagt
     4921 atcaataccc atgaatcttc gtggaatgtc tcgacatggg tccataatat cggatcaggt
     4981 ttaggttaca gtggtttttt attgtttcca gttcttcttt tcttgatcta tcgaaaaaat
     5041 gggcaaatgg acgtaagcca cttatacctt ggactgtctg tgatcagttt gctttttgcg
     5101 gcgatttatg gactggcaag aattccttct ataaattctt ggccattact gaataaaata
     5161 ggtttttgcc aaagaatcag ttttttcttt aattatgtgc ccattgcctg gttgagtatc
     5221 ttgcatctca aacagtaaaa cttgagccgt caaattgaac agtcagcgga tacttcatcc
     5281 ggtggctgtt ttttgttttt ctgaaatata tatttccaca gttttcttgt atagcttctg
     5341 ttttagagaa gaaaggagct atattttgtg ctacaatctt aaaaaggtag agagaagacc
     5401 aaatggaaga cagaggagcc actaaaatat gagaaacatc aagttgacaa tcgaatatga
     5461 cggaaaaaga tattcaggat ggcaaagact tggagatgac gataaaacca ttcaaggaaa
     5521 aatcgaaaaa gtattgcatc agatgacaaa tgaagagata gaaattattg gatcaggaag
     5581 aacagatgct ggcacccatg cgcgaggaca agtagcaaat tttaaaacaa acacagaaat
     5641 tggactggca gaaatgatcg actttatgaa tcgttacctt ccgcgggata ttgtcatcaa
     5701 aagaatcgaa gaaatgcctg agcgcttcca ttcaagatat aatgcggtgg gcaaaaaata
     5761 cagctattat gtttggaaca acgtgattcc ttcagcattt gagcgaaacc acagctttta
     5821 tttccctcaa gagttggata tggacaagct gaatgcagct tgcgaaaaat tagtgggtac
     5881 acatgatttc attggttttt ctgcattgaa aaaatcaaaa aaatccacag tgcgtacgat
     5941 tgatgaatta tctatcgaac gaaagggaga aatgcttcat tttacttttg tcggagacgg
     6001 attcttatat aaaatggtgc ggattatcat ggggacatta ctagaaatcg gcacaggtgc
     6061 catgccgata gaaacaatcg atgaaatatt tgaaagcaaa gtgcggcaac atgcaggaga
     6121 aaccgtccct gcacaaggtc tgtttttgga tgaagtttat tatcgataaa aatactttga
     6181 taatggatga aacgcatgtt tcacagtata aatatcgatg ttaaataatg tatgaagcaa
     6241 attaaagaaa tataaaaaaa gactttttag cctgtttatg tgtacagcta aaaagtcttt
     6301 cttaaataaa aatctagtga gaatgttgta cttctcttct cggttgtttt tgtttagaaa
     6361 aagtaaatcg ataaagtaaa aataaaatca aagtaacgat agataaatat cttgaagcaa
     6421 ttgctagttt tgtgtactga taagtaataa cgactttatt tatacctgga ggaatagtta
     6481 attcagttgt gccaaactta gataattttg tttgtactag tttgccatta atagaagcca
     6541 cttgcccttt ataacgataa ataggtgtcg tcaagacagt atttttatta ttcgaattat
     6601 ctaattcaat tgtaagtttg ttactcataa aatgagttgt tggatttatt tcctggttac
     6661 ctagtaaata ttgatcatta ttaatcatat caggatgata gattgactct ttgtttgcat
     6721 aatcagtatg attgtaattt tcagctatag caacagcgtc ttcaggtgct ataagtgtta
     6781 atgaatcttt gttagcatgg atagaattgc ttactcctga gagatgcaat attagtgcaa
     6841 gaaacgtaaa gaccattatt ttccatgaat tatttttagt agaaaaaata taatagatca
     6901 ctatactcat cgtataggct ataaataaag aagaaaaacc atttaaccgc cagacaaatt
     6961 gtagactctt aactggtgta tggttaaaaa gttgccaagg aaataaattg gttgaacaaa
     7021 aaagaacaaa tacaccaaga aagaaaataa ataaatca
//
LOCUS       DAYYHX010000145         6885 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000145 DAYYHX010000000
VERSION     DAYYHX010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.146"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHA6Y_002163"
     CDS             complement(<1..182)
                     /locus_tag="ACHA6Y_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8988241.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHA6Y_002164"
     CDS             517..951
                     /locus_tag="ACHA6Y_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8988242.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHA6Y_002165"
     CDS             974..1294
                     /locus_tag="ACHA6Y_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988243.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHA6Y_002166"
     CDS             1435..1626
                     /locus_tag="ACHA6Y_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988244.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACHA6Y_002167"
     CDS             complement(1697..1954)
                     /locus_tag="ACHA6Y_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM8988245.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACHA6Y_002168"
     CDS             2112..2375
                     /locus_tag="ACHA6Y_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988246.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACHA6Y_002169"
     CDS             2399..2587
                     /locus_tag="ACHA6Y_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988247.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACHA6Y_002170"
     CDS             complement(2670..2855)
                     /locus_tag="ACHA6Y_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988248.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACHA6Y_002171"
     CDS             3070..3441
                     /locus_tag="ACHA6Y_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8988249.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACHA6Y_002172"
     tRNA            complement(3591..3663)
                     /locus_tag="ACHA6Y_002172"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACHA6Y_002173"
     CDS             complement(3709..4137)
                     /locus_tag="ACHA6Y_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988250.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACHA6Y_002174"
     CDS             complement(4197..4955)
                     /locus_tag="ACHA6Y_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8988251.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACHA6Y_002175"
     CDS             5250..5447
                     /locus_tag="ACHA6Y_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988252.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACHA6Y_002176"
     CDS             5553..6035
                     /locus_tag="ACHA6Y_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988253.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACHA6Y_002177"
     CDS             complement(6093..6689)
                     /locus_tag="ACHA6Y_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8988254.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2331 a         1058 c         1353 g         2143 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttt
//
LOCUS       DAYYHX010000146         6805 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000146 DAYYHX010000000
VERSION     DAYYHX010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.147"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..2414
                     /locus_tag="ACHA6Y_002178"
     CDS             120..2414
                     /locus_tag="ACHA6Y_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM8988255.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            2407..3072
                     /gene="pgmB"
                     /locus_tag="ACHA6Y_002179"
     CDS             2407..3072
                     /gene="pgmB"
                     /locus_tag="ACHA6Y_002179"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8988256.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            3065..4072
                     /locus_tag="ACHA6Y_002180"
     CDS             3065..4072
                     /locus_tag="ACHA6Y_002180"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8988257.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            4094..5113
                     /locus_tag="ACHA6Y_002181"
     CDS             4094..5113
                     /locus_tag="ACHA6Y_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8988258.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(5394..6761)
                     /gene="nhaC"
                     /locus_tag="ACHA6Y_002182"
     CDS             complement(5394..6761)
                     /gene="nhaC"
                     /locus_tag="ACHA6Y_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8988259.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
BASE COUNT         2221 a         1417 c         1346 g         1821 t
ORIGIN      
        1 tctcctaaaa atttattttt ttaagctttt ttctatgaaa tattttcttt tgtctattga
       61 cttgtgcaat cggttgcgtt tattatgaac gtataaaaca gctcattagg aggagaaaaa
      121 tgaaacagat caaacgacta tttgctattg atccatggaa aatcagcacg acacaattag
      181 ataaaaaaaa tcttcgactg caagaatctt taacaagtat tggaaacggt tatatgggga
      241 tgcgcgggaa ctttgaagaa acttactcag gggaccacca tcaaggtact tatttggcgg
      301 gtgtctggta tcccgataag acacgtgtcg gctggtggaa aaatggttat ccagaatact
      361 ttggaaaagt gatcaacgcc ttgaacttta tcgcgataga tatttcgatc aacgatcagc
      421 tgatcgactt ggcttctttg gaaccagaag acttttactg ggaattagat atgaaaaatg
      481 gtgtattatc tcgtacattc acaatcacta cttctactaa taaagtacgc atttccttcg
      541 aacgttttct gagcattgtc aaaaaagaat cagcttatat ctatttaact atagaaatgc
      601 ttgaaggtag cggaacagtc aaactcgtat caaaattgga tggaaatgtt caaaacgaag
      661 acagcaacta tgatgaacat ttttggaatg agattgatcg tggaactata gaagaaatcg
      721 gctatttgac gactaaaact attccaaaca actttgggat tgaacaattt actgttactg
      781 cagcaatgcg ccatttcgtt aatggaaaat cagtcatccc tgactatcac gaagatactt
      841 tagcattaca tggttgcttg tctgcagaat tatctcaagg tgagacatgg tcagtggaaa
      901 aagaaattat cgttcttact agtagagata ttccagaagc gcagcaagca gaaaaagctg
      961 tctcagaatt aacgaaccag acaaacaatt atatccaagc aaaagaagag caaacagcgg
     1021 cttgggcaga acgatggaag cttgccgatg tggtcatcga aggagacgaa gaagcacaac
     1081 aaggtattcg attcaatttg ttccaactat tctctactta ctacggcgaa gatgaacgat
     1141 tgaatatcgg ccccaaagga ttcacaggag aaaaatacgg cggagccact tactgggata
     1201 cagaagcgta tgctgtccct ctttatcttg ctttagccaa acctgaagta actaagaatc
     1261 tgctgaaata tcggcataat caactgccgc aagcaatcca caacgctcag caacaaggat
     1321 taaaaggcgc gctttatcct atggtcacct tcactggtgt cgaatgccat aacgagtggg
     1381 aaatcacatt cgaggagatt caccgaaatg gcgcgattgc ctatgcaatc tacaattata
     1441 cgaattacac cggcgatacg gaatatttga aaaatgaagg actggaagtt ttagcagaaa
     1501 tcgcacgctt ctgggcagac cgtgtccatt tttcaaaacg agcaaataaa tacatgatcc
     1561 acggtgtgac cggcccaaac gaatatgaga acaatatcaa taacaattgg tatacaaata
     1621 cgatcgcagc ttgggtcttg cgttacacgc gagaaaatta tttaacttat caaaaagaag
     1681 ctgctatcac tatcagcgaa gaagaactag ccaaatggaa agatatcgca gagaacatgt
     1741 acttcccagt tgacgaagaa ttaggaatct ttgtccagca tgacacattc ttagataaag
     1801 atctcatgcc tgtatccgat attcccacaa gcgatcttcc tctgaatcag cattggtctt
     1861 gggacaaaat ccttagatct tgcttcatca agcaggcaga tgtactacag gggatttatt
     1921 tcttcaatga tacttttaca aaagaagaaa agcaaaaaaa ctttgatttc tatgaaccaa
     1981 tgactgtcca tgagtcttcc ctttcgccaa gtatccattc gattttagca gcagaattag
     2041 gaatggaaag caaagctgta gaaatgtacc agcgaacagc tcgcttggat ttggataatt
     2101 acaataacga cacagaagac ggcttgcaca tcacttccat gactggtagc tggttgtcga
     2161 ttgtccaagg atttgctcaa atgaaaactg cccatgatca gttgagattc gcaccatttc
     2221 tgccaaaaac ttggaaagct tattctttcc atatcaatta tcgcggacgt ttattgttca
     2281 tctcagtcac agatgaacaa gttactttcg agttattaga aggttctcct ctagaaattt
     2341 acgtctatga acagtcttat tctttagcgg atcgtctaga aataacgcta agaaaggagg
     2401 aagcaaatgt ttaaaggggt attgtttgat ttagacggtg tcattaccga tactgctgaa
     2461 ttccattatc gtgcttggcg aaagcttggt caggaaatcg gcattacgat cgaccacaca
     2521 ttcaacgaac agctaaaagg cgtcagccga gaagactcac tctctttgct tttagcttat
     2581 ggcggaaaag aacactcttt ttcaaaggaa gaatttgcag aattagcgaa aagaaaaaac
     2641 gattattatt tagagatgat ccagacgatc gaaccaaaag atgtcttccc cggggtcgtg
     2701 cctttactgg attctttaaa agaagaaaaa atcaagattg ctcttgcttc tgcaagtaaa
     2761 aacgggccat tcctacttga aaaaatgggt cttacacctt actttgatgc tattgccgac
     2821 ccggcagaag caacaaacgg aaaacctgcg ccagatatct tcctccttgc agcaaaagct
     2881 gtcggactaa aagcagaaga atgtatcggg atcgaagacg cgcaagccgg tatccaagcc
     2941 atcctctcaa gtggcgctca accaattggc gtgggacgtg ctgaagacct tggagaggag
     3001 atcccgcttg tccctgatac gacttttctt acgatcgatt acttgaaaga aaagtggcat
     3061 gaccatggat aatccagaga tctccgtcgt accagttcca gagactgaaa taaagaaaat
     3121 cacattgaag aacgactact tagaagtcgt tcttctcaat tacggtgccc gcctgcacca
     3181 aatcttcgca ccagacaaag aaggaaaatc agaaaatgtt ttattgtctt acgatcgttt
     3241 cgaagatgtt ctaacagaca aaagcttttt tggagcaact gtcggacctg tagccggaag
     3301 gatccgggat gcttcttggg gtgagcatag attggagaaa aactgcggat cacaccatat
     3361 ccacggtggc acgaaaggct ggtctttcca attttgggaa gtagaagttt tcaaaactcc
     3421 atcatcaata ggtgtggtct tttacttaaa agatgaattt tctacctatc caggaccaat
     3481 cactgctacg atcacttacc gtttaacaaa aaacgaatta gagatgacaa cctctgctag
     3541 tagccttgtt gaaaccatct gcaatccgac caaccatgcc tacttcaacc tttcaggaaa
     3601 tggaacaaga gacatctatg aacatcagtt aacggtcttt ttagatggtg tgcttgaatt
     3661 agatcaagaa aaactgccta ctggaaattg gacaaaaaaa gaaaaactgc caatcgactt
     3721 tagaaaatcc ccaacactgc aagagatcct tgcatgttat ccagatggac tagatgatgt
     3781 atttcttttg catcatccta ggctttccaa aacttcctta caattattcg agaagcattc
     3841 tggtcgccaa atgactatag caacttctaa taaaagtatg gtattatttt ctacgactgg
     3901 attcgaagct gatttctctg tcaatggaaa acagatgcat tcaaactacg gattagcaat
     3961 cgaaccgcaa gaaatccctg atatcgttca tttccccaaa tttggatcga tcaacctcca
     4021 tcctggtcaa gaacggatca gtcagacgat ttatcggttc tctgcacaat aacaagagaa
     4081 aggtgttttt atcatggcta ttaccgtaaa agatgtggcg aaaaaagccg gtgttgccac
     4141 ctcgaccgtc tctcgtgtca tcaacgatca tcctagtatt tccgagacaa caaagaagaa
     4201 agtccgaaaa attatggatg aactaggtta tgttcctaac atgactgccc gcaatctagg
     4261 aaaacgcatt tccagtgcca tcggcgtaat cttaccccct cttgattcaa aagagcggat
     4321 cggtaaccca ttttatcttg agatcatgga agcaatcaac gaagaagcgc ggaattatga
     4381 tatgactaca gcaatcgcta ctgctaaaaa ttttgaattg ctgttagaga acgtccagcg
     4441 tatgcattta caaaaacaag tagatggttt tattctagtg tattcagata gtcaagatac
     4501 tgtcattgat tatttatacc aaaaccacat ccctttcact ttgatcggac agccttatca
     4561 tcatgagaac gaaatcgttt atgtagataa tgataaccag cttctaggta aacaagcaac
     4621 agaatttttg atccagaatg gacataagaa tatcctgttt gtgacaaaca cgactcatga
     4681 aagtttgtat ttcgaacgat atttcggtta tcaaaaagca atgatgatgg ctggtcttcc
     4741 tgcgttatcc agtgtagtac tagagcatcc ggaggattat ctgtcctttg agaaaacgtt
     4801 gaaagaaaac gaagccacag cattggtggt cattgacgat atcttcgccc ttcgcacgat
     4861 gcagttggcg caaatgtatg gctatgtcat tccagagaca ctctccgtca ttagcttcaa
     4921 caactcgatc ttctcaacat taacacaccc ttaccttaca agtatcgata tcgatatctc
     4981 cgaattagga cgtgtcgcta tgcaaaagct gcgcgaattg atccatgaag aattgagcag
     5041 tggcgtacgt ttggtcatcc ctcatcgctt gatcaaaaga gagacagtcg ttcctttaga
     5101 aaacccatac taaatagctg actcactaaa tacgctgacc atttatccta tggtcagctt
     5161 tttatgtaaa agaagatact acttctaaca cattgtttat ctgtaaaaaa gagcgtggga
     5221 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag
     5281 caactccttc ggaaataagc tgaaattcac aaaaatttaa aaagcaattt tcttgagttt
     5341 cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttcctt ccttcaattt
     5401 tttacacgtc gacgattcaa taaaaaaccg cctaatattg taaagacagg tgcaagaatc
     5461 gagaaaaagg cgaatggcaa gtaatgcaac gcgctaactt tcatcgttcc cataatgaat
     5521 gtgccgctga ctccccacgg tacgagagaa ttgacggtcg cacctgaatc cgctagtgtt
     5581 cgtgtcaaat aggtttttga taagccaacg tcatcaaaag aacgcttgaa tgtttctcct
     5641 ggaagaatga tggacagata ctgttctcca acgatcagat tgattccgat acagctcaag
     5701 gcagtaaacc cgactaaacg agaaggtcgg tccatctttt cttttaattc ttgaacgatg
     5761 gtatcaacga tcagatattt gatcagtaac ccacctaatg ccaatgcaag taaaatcaaa
     5821 ctagcagaac ctaacatact catgatcccg cctcgagaaa gcagttcatc tagctttgga
     5881 tcaggtgtat ttgcgacata accattcatc aagatgtctg ctgcttttcc gaccgtcaaa
     5941 cttttctcat ggataaaaga aagaacgaga gcagctgtcg atcccgctag taatgtaggg
     6001 atagccggaa tttttttgat tgccccccca aataaaataa ctagtggaat caatgtccaa
     6061 ggagagatcc aaaagctcgc tttcatgttt tggatcattt cattgatact agctagatcg
     6121 gctgaatgac tgttatgtcc taatatcgta taaaaaatca acgaaaggac gaatgctggg
     6181 aaatccgtat aacgcatacc agcgatatgt tcgaatagat taactcctcc aatacccgct
     6241 gccaagttgg tcgtatctga taaaggcgag cagttattcc ctaaaaaggc accggaaacg
     6301 actgcgcctg tagtcattgc atcattgaat ccaagtaaat gaccgatccc caaaaaagca
     6361 attccaatcg tcgaaatcgt tgtaaatgaa ctgccgacag tcactccgac aattgcacaa
     6421 acaagaaatg ctgtcggtaa aaagaaacgc acagaaacaa gtttaaatcc ataaaccata
     6481 atcgtaggga tggttccaga taaaatccat gaagcaacta agacaccaat caaaagaaaa
     6541 atcaggattg gaatgatccc tggagtgatt cctttgatga tcccgtcatg gatctcatcc
     6601 catgtgaact ttttaaatct gccataaaac aacaaacaca tgaaagctgc aaggatcggg
     6661 atatgtggcg agagctggaa tccgatgatc agtactccta ggagtgccaa caacacgatc
     6721 aatacagcga tactttcttt taaagaaata tttttattca tcgtcgatct cctatttcaa
     6781 atgaaaaggc cctgccatga aaaaa
//
LOCUS       DAYYHX010000147         6771 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000147 DAYYHX010000000
VERSION     DAYYHX010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.148"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..895
                     /locus_tag="ACHA6Y_002183"
     CDS             5..895
                     /locus_tag="ACHA6Y_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM8988260.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(968..1792)
                     /gene="nadE"
                     /locus_tag="ACHA6Y_002184"
     CDS             complement(968..1792)
                     /gene="nadE"
                     /locus_tag="ACHA6Y_002184"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM8988261.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1804..3276)
                     /locus_tag="ACHA6Y_002185"
     CDS             complement(1804..3276)
                     /locus_tag="ACHA6Y_002185"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM8988262.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3913..4275)
                     /locus_tag="ACHA6Y_002186"
     CDS             complement(3913..4275)
                     /locus_tag="ACHA6Y_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8988263.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4498..5250
                     /locus_tag="ACHA6Y_002187"
     CDS             4498..5250
                     /locus_tag="ACHA6Y_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8988264.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5390..6313)
                     /locus_tag="ACHA6Y_002188"
     CDS             complement(5390..6313)
                     /locus_tag="ACHA6Y_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8988265.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFLDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6328..6600)
                     /locus_tag="ACHA6Y_002189"
     CDS             complement(6328..6600)
                     /locus_tag="ACHA6Y_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988266.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2068 a         1349 c         1156 g         2198 t
ORIGIN      
        1 atatatgcaa ctacaaataa acgaagaatc cttaccggta tatgaagcat tagctagtaa
       61 aacaaggatc aagatcatcc agctactttc caaaaaaaag atgaatgtca aagatttggc
      121 aaaagaactt ggagtgagca gtgctatcac gacgatgcac gtgaaaaagc tagaggaagc
      181 aaacatcatc aaaacagaaa aagtaggaca acaaaaaatt tcgagccttc gtgtcgataa
      241 aatcgatatc agttttccag aaaaaatatt caatgctttt gatacgaaag aaacatcgat
      301 acctattggt cactatacaa attatgcaat tgaaccaaca tgcggtttag ctacgatcca
      361 tgattttatc gggaaagtgg atgaaccacg ctattttatg gatcctcgaa ggatggatgc
      421 ccgcattctc tggtttacca gcggatttgt cgagtatcag gcacctaatt ttttaaatac
      481 agaggataca ttggagatgt tggaagtttc tgtggaaatt tcttctgaat ttcctttttc
      541 aaatgacaat tggccttcag atatcacttt cagcctaaat ggtgtggagc taggaacatg
      601 gacgagccct ggagattttg ctgatatccg cggtaaatat acgcctgatt ggtatccaga
      661 taatcttaat caatacggct tgctgaagac gatccgaatt accaaacact tgacgaatat
      721 gaatggagaa cccttgtcaa acatcacgat caacgacatc cctaaagagc aagacacttg
      781 gcatttgcga atcgaagtga aagacgatgc caaacatgtg gggggctgta ctttatttgg
      841 taaaggattc ggtaattacg atcaggatat caaattaaaa gtttactaca gctgatattt
      901 ttcttttcta ttttataaaa caaaaacagc caatccctat gaagggacgg ctgtttttgc
      961 tttattgtta tttccagaaa tcatccaaaa cggaaattgg caaatgtcgt ttatgttgtg
     1021 tcttgttcca ccatgcttcg attttttctt gtgcttgagc gggaacttct tttccttcta
     1081 gataatcatc aatttcatta tatgtgacac ctagtgcgac ttcatctgca atcattggtt
     1141 tcccatcttc aagatctgca gtcggaattt ttgtgtataa tttttccggt gcattcaatg
     1201 cttgaagcaa ttgtttccct tgtcgtttgt ccaaacggaa taatggcaaa atgtcggctc
     1261 caccatcacc aaatttcgtg aagaatccag taatgttttc tgcagcatga tctgtcccta
     1321 atacggctcc tgctctttct ccagctacgg catattgcgt aatcatacgt tgacgtgctt
     1381 tgatatttcc tttgttaaaa tcgctgattc ccacacctgc ttcgcttaat acttgtactt
     1441 gtgcatcaac agctggtttg atatttactc gcagactaac atcgggctgg ataaagtcaa
     1501 gagcagcttt tgcatcttct tcatctgctt gttcaccata aggaagacga acagcgataa
     1561 attgatagtt ttcatctttt gtttcttcac gcatttcttc catcgttagt tgcgccaaac
     1621 ggcctgctaa agtagaatct tggccgccgc tgatacctag tacaaaagtt ttaagaaacg
     1681 gatatttttt caaatatgct ttcataaagt caatactttt acgtatttct tcttttggat
     1741 caattgttgg tttgacacct aattcattga taatttctgt ctgcaggctc atttattctc
     1801 ctcctatgct tctgtatgaa gttctgctac gctttttttc attcgttcca tgatagccat
     1861 tttatggttc cagcattcag tagacagatc gactgggtat ttttgcggat tcaagtctcg
     1921 tttatactct tcccatagtg aatctagatt ttcttttgta tattctttga tttcgtttag
     1981 tgcaggtaat tcgtaaactc tttttccttc gataaagata tcttgtaaaa ctgggcgagc
     2041 tgtaaagtct cgaacagttt tattgataaa cgtatgcact ggatggaaca tgaagatagc
     2101 ttcttcttct cgagggtctt catcccataa tgtcacgtag tctccttcag atttcccatc
     2161 aaaatttcga gtgatgcgcc acacttgttt tttccctgga gtagttactt tttctgcatt
     2221 gcttgataac ttgatcgtat ccatcatttt tccttcatca tcttcgatcg atactagctt
     2281 gaatactgca ccaagggctg gctgatcata ggctgtgatc aattttgtcc caacacccca
     2341 tacatcaatt ttagcttttt gcatctttaa attcaaaatc gttgcttcat ccaagtcatt
     2401 cgaagcatag atttttgctt ccgtaaatcc ggctgcatct aattgttccc gaacacgctt
     2461 agatatatag gccatatctc cactgtcgat gcgtacgcct tggaagttga ttttgtcacc
     2521 taattctttc gccacgcgaa tcgcacttgg cacacctgat ttcaacgtat cataagtgtc
     2581 gactaagaac acgcagtctt tatgggtttt agcgtaggcc ataaatgctt cataatcatt
     2641 tccataagat tggactaacg aatgagcatg ggtcccgcta gctggtatcc caaatatctt
     2701 cccagcacgt acattgcttg ttgcatctgc tccgccgata tatgccgctc tagttcccca
     2761 gactgctgca tccagttctt gtgcccgtct agttccaaat tccaatagtg gatcttctcc
     2821 gatgacagat ttgatacgag ctgcttttgt cgcgatcaac gtttggaaat tgaccatatt
     2881 caacagtgct gtttctacta attggcagtg cgctaaaggc ccttccactt ggattaatgg
     2941 ttcatttgca aaaactagtt ctccttcacg agcagaacga actgttgctt taaattcaaa
     3001 attttgtaaa tactccaaaa aaccttcagg atagacttct aatccacgca agtaagcaat
     3061 atcggaatca ttgaacgtca gattctctaa ataattgact aaccgttcca atcctgcaaa
     3121 aaccgcgtat ccgtggttaa aaggcatttc tcgaaagtaa cattcaaaaa cagcatgtaa
     3181 atcagctcga cctaattccc agtatgtctg catcatattg atttgatata gatccgtatg
     3241 cagcgtcaaa ctgtcgtctg ggtaagtatt attcatcttg tattactcct cttggcaagt
     3301 tttcatttcc tacccattat aacaaaaaaa attaatagga aaagaaatct tccatttttt
     3361 ctgaaaaagc acatgtttta cggatatttc ttgctaaaaa tgaactccat ctgctaaaac
     3421 tatcctttct tattggtatt taacctaaac gagaaagaat acttatttta ttcaatgtat
     3481 ccgagacacg aagaaattag cgttttctat ttttcttact tgataaaata agtagatttt
     3541 cctactacaa tcgtagccgt taagataccg atgattgaac aaatataacc aaatgtctct
     3601 acaacataat caatggcaaa agtttttgaa gcaataatac gtctttcctt ttttgcttct
     3661 tatccccaaa gaaacggata taacttatta tgtattgaat ttttttggaa aggcgaatta
     3721 tgacaagata taccttgcgt tgaaaaaact cgcggaaaaa atatcacacg tttttaaaaa
     3781 gatacaaagt aagaaagcag ctgtcaatag aatggaaaca aaaatggcga gacatcaatc
     3841 agtctcgcct tgttttttat tgtatacatt agatatggag caggttacca taaagatgat
     3901 tattagtcgt tattaaatag aatccttcgt atttttttgt tctatcacta caatatcttt
     3961 atttggacgt ttgaattgta ttccggcacg ttctagctcc tgtttcactc ctacatggac
     4021 tttgacatct tctcgtgtca tttttaaaac gtgatgaatc gcatagtcga tttcttgcct
     4081 tgtgattttt ttcttcgtat tgatcatcac gacttgcttt ttatcatatg tgtctaccaa
     4141 tagcagttgt gttcgatcta acgaaaataa tttgtaataa ttaatagccg tattcccaaa
     4201 aaagaattcc actactttag gataaaatgt cttaatattt aaattactat tcacaggtgt
     4261 ttcgattagc ttcatctcat tcctcttttc tatatagtcg atttagatcc aatcttaagt
     4321 tatgttacca tctttcctag cattaaggaa tcggttacaa ttgaaccttt aaagacaaaa
     4381 ggatttacct tttatcactt aatagtataa cataatttcg tgagaaaaaa atacaatttt
     4441 ttcttgacca tctcgttacg taaaggatta tgcttacatt tgtcaggagg aaaaataatg
     4501 gagtacacaa taaagaaaat gtcagagata tcaggagtca gtccgagaac cttgcgcttt
     4561 tatgatgaga ttggactgct aaaaccagct aggatcaatt cttcaggtta tcggatttat
     4621 ggcaaaaaag aagtcgatcg tctgcaacac atcttatttt atcgaacaat ggcattcaag
     4681 ttggatgata tccaagaggt cttagacaat ccatcttttg accaccagaa agctttaatc
     4741 aagcatcagc aaatgttgct ggaaaaacgg gcacaaatcg ataccctctt gacaactgtc
     4801 caacagacac ttgatatgta cgaaggagga agaaaaatga gtgacaaaga aaaatttgaa
     4861 gggtttaaac aacaaaagtt gaaagaaaat gaagaatcct atggacaaga gattcgacaa
     4921 aaatacggaa aaaaaaaggt tacagcatcc aatgaaaaat ggatgaatat ggataaagaa
     4981 acgtatgaag aaatgcaaac tgttgaaaag cagatgttat ttgatctcac tctttactta
     5041 aaagaaccag aagatgaaag cttagcagac cgtatcttcc aagctcataa gaagtggttc
     5101 tcttacagct ggcctgaata tcagccaaaa gcgcataaag gactaggaat gatgtacgaa
     5161 gccgatgaac ggttcactgc atattatgac gagcgaagtg gtcatggtgc agcaaaagct
     5221 ttaaatgaaa ttatccaaaa aaaggcgtaa tcttttacat gatccaacaa aaaagtacac
     5281 gtaaaatgac ctaataacaa aacagcagac acccaagtta gatttttatg ctaacttttg
     5341 gtgtctgctc cattctttca caagtttttt catcctactt taaatatatt catcgttgaa
     5401 taaacgtata aggtattttc actttatcct ttttttctgt aagaaacaat tgaaatgcca
     5461 tttctccgca agcttccaga tggtggtcga tggttgggaa accaagaagc tcgctgatca
     5521 gcaaattatc gcgtccaatc accactggtg tcttatcttg cagatagctt tgcaagatgc
     5581 ctgctgcaac catatccgaa tttgtaaaga caccatcaac tccaagctct ttaaaaaaga
     5641 agcctgcttg ttttcctttc tctgcatcta tacaatccaa aaaaacaagt gattcatcaa
     5701 aagatggaaa aagttgatga catagctgca aagtgatttt tgagttataa cttagcctgc
     5761 cacttcgccc caacgtgatg cccaattgct ttataccttg ctgcttcaaa taagtaagac
     5821 cttctgtaat tgattgttct cgatcaataa agacacagct tgcctgtttt cccttgattt
     5881 cttcacaaaa aactagaggt ccatattttt gatatgccaa taaatcatct agtgtattgg
     5941 cacgagacgt aatgatcaat ccatcgaaag ctttagctgc aaattcttca agataccttc
     6001 gctctttttc tttatgatag cccgtcggta aaagtgtcac tttgtaatca taatcaaacg
     6061 ctgcttcaat gatcccactg atcaactgat caaaatacgg atgattggta taaggcagaa
     6121 tcactccaat gcttttcgtt tttccataac ttaactgtct ggcatttgca ttagggatat
     6181 aatcgacttc tttcatgacc gcaagtattt ctttcctttt ttcctcatct acatatggat
     6241 gtccattgat tacacgtgaa atagtagaaa ctgaataacc tgtgagcttt gcgatatctc
     6301 gaatgtttgc cactgctgct tcgccaccta tacaaaaaga ttttcttgta aatagtctac
     6361 cacagctgga ccgcattcaa aaaactgata catttttcct tcatgcgaaa aactgatccg
     6421 ctgcccgccc caatatatat cttttgtcaa attttcgatt tcattcattg gtatttcttt
     6481 tactatattc ctattcaacc cttgaaaact gtacaacgtt aatacggatc ccttgaactc
     6541 gacaaacgca taaaaataat tgttcgttgt gccatctgtt atctctacat aagtattcat
     6601 tgcaagccct ccttttgata ctttgagtat agtgtatgaa acgcgtctca tagcaaggag
     6661 tttgttttgc caaagaaaag ttttattcct ctttcattat taccaaagtc tcttatttta
     6721 aattgaatac attataatga tactgtaaat atatctttat tattagtgaa t
//
LOCUS       DAYYHX010000148         6732 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000148 DAYYHX010000000
VERSION     DAYYHX010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.149"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACHA6Y_002190"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACHA6Y_002190"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFM8988267.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACHA6Y_002191"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACHA6Y_002191"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFM8988268.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3872)
                     /gene="iolC"
                     /locus_tag="ACHA6Y_002192"
                     /pseudo
     CDS             complement(2837..3872)
                     /gene="iolC"
                     /locus_tag="ACHA6Y_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
     gene            complement(4029..5045)
                     /locus_tag="ACHA6Y_002193"
     CDS             complement(4029..5045)
                     /locus_tag="ACHA6Y_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8988269.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5316..6635)
                     /locus_tag="ACHA6Y_002194"
     CDS             complement(5316..6635)
                     /locus_tag="ACHA6Y_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFM8988270.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
BASE COUNT         1773 a         1639 c         1159 g         2161 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa agcagcgctt
     3181 tcattatctg acatgttagg ctggatcaac ttctcagtta gatcgaactc ttctcttgaa
     3241 cccatgataa tgtctgattc cttagcaatc atcgaatagt aaattgaaat atcatcgttg
     3301 ttcttccagt tataagcacg gtaatcaata tcaaaaataa ttttgacatt attccttttc
     3361 gccagcatca ctgccttgat tacggcttct ctggacgggc tctctgctag tgcagttcct
     3421 gagattaata gtgcttttga ccgtttcaag tagtcctcat caatatcctc aacgctaagc
     3481 tgcaggtctg cggcttgatt tcgatacatc aaaatattac tttcctttga tgagagcatt
     3541 tcggtaaaag tcagccccag cttttcacca tgctgcgctt tgctgatctg agacgtatcg
     3601 atgccttgtt cattgaaata atcgatcaca tattcgccaa actgatcgtc cgagactgtt
     3661 ccgataaaac cagctttcat gccatgttta gtaacaccca cagcaatatt ggcaggagat
     3721 ccgcccacaa atttttcaaa catatggacc tttttcaatg gtttaaactc ttctttgact
     3781 tgatcattgt aggcaggatt aaaatcaata gcgactcttc ctaaaagcag caagtcaaat
     3841 tcttttgatt ggtcaaactt tatataactc aatatatcca cctctttatc cgttcttcta
     3901 tttctgaggc aagtatagca cgccaaaaca aagcgtgcaa tatttatttt agaacgcaat
     3961 attgcgtgct gtttgaaaat gacggcaaaa aaaacgtgaa tcgccttaca tcacgattca
     4021 cgcctagatt aaatattgta ttttgtctta ataattatat cagtaaatag cgcatctttt
     4081 gtaaccgttt tattattttg cagctgattc gctaacatca gtaatgaacg atagccttga
     4141 acaaatccat cttgatcgat tagaaaatca acctgatcgt ttttcagcat ctcgatattg
     4201 ttcttagtca aatcataaat gattacaaag ggccgcttgc tttcatcgat tttttccaac
     4261 gctcgggcaa ttcccgcttg tccgccagaa aagacgacta cgccatctaa gtttgaataa
     4321 ttctccaagg tgttgcttaa aatcttttca acctcagcac tcgaatcaaa gctgctctga
     4381 acacccgtca gctccagttc aggaaacgac tctttcaatt cttcaacaaa gcctgccacc
     4441 cgtaagctgt tgacgctatt tccaaagtag ccggtaatcg ctagaacctt gccctttcct
     4501 tttgtcagca tacctaacaa tcccgcagcc gtttttccgc ttttgaagtt gtccatccca
     4561 acaaaacact gtcgattcgt accaagaata tccgaattaa aggtaatgat gaggatgcct
     4621 tgatcggtca attgattaat tttttctctg ataccatcac tatctactgg catgatcgcg
     4681 atccctttta ctccaagctc caccaatcga tccaacgcct gcagctgggc cgcttcatcg
     4741 accccgtcta cctcttctaa aaaacaatca atgtttcgct tttttaaatc cccgataata
     4801 gattccagac ctgatcggat cggaatcata aaagaagatt tcgccaattg agtgacaatc
     4861 cccagcttga gcgttgtctc ttttatgacc ttctttttct ttggaacata gttcatttcc
     4921 ttggcgattg ctctgattct ttctgcaacc tcaggattga tccttcctcg atcgttcaat
     4981 gcccgatcca ctgtccccct tgacactcct gctttctcag ctatttcttt aattgtaccc
     5041 gccatcttgc ctctcctttt ttcctattgt gtttatttta tcacaaaact taagaaaacc
     5101 accggagaat gaattctccg gtggactttt tgtatgatta accttcttat tgaacatcac
     5161 tgatcttaaa ctctattcca agggcgtttc tataatattc tggatacatc tccttcccac
     5221 atttttcaca ggtaaattgg ggaggattgt tcggatcatt tgcaatatct gcatcatcta
     5281 aaaattttac tacatccatt gggattgatt ctttttcacc acaacttaga catataaatt
     5341 tcacgttggc gttcccaatc ccagcgaatt gctttaaaag ttttttcttt ttcttcttca
     5401 tactgttgcc tgaattcttt ttggtcaatt tttcgattct cctctcgcat aaaccgcttt
     5461 gcatggttat ctttcgcata tttaataact aaccgtccat ttttgcgcac actcatccca
     5521 acgaattcat agtactcacc gcaacaagaa cattctaagg gattttcacc aaattcttcg
     5581 gttattcttt cttcccagga ttttggtctt aaggcctcat tcagattgat aaacagcttt
     5641 tttactcgtt tctgatactc cttcatgacc tttttcatta atgttttgat tctacgacta
     5701 tagattccat agcgacgaat cgttttgaaa ttcttatttt gaatatggcg aatcagtgca
     5761 gctatgaatt cgtctatact catggtcatc gtctctttag tattcgttcg tttgtccatg
     5821 tagttgaaca tgacagtgtc gccatcatac attaagattc ggcttaatgc gatcgggcca
     5881 cgcttcatat aacgactgat gtattggagt agtgacttta gatttgtcct gctcctttta
     5941 ggagcattta cgtaaaaccc ttctccattg ttcttgtacg cagcttgtaa tcttgattga
     6001 actctttgct tgtcccactc acctaacgtt cgtctaatta gtttcaatac agcatttttc
     6061 cagtattttc ttaacataga aaaaggtaaa taatcgtatt cttcccaact tccatctctt
     6121 gttaagccac ccatagtaac aaccatatga acatgcggat taaattctag ttttgatcca
     6181 aaggtgtgga ttgtagctac tatacctgcc ttcacctttc ttttctcaaa ataggcaaga
     6241 ataatttttg cggcttcatc cattagtcct ttcagtaagg aatctcgata gttttccatc
     6301 ataaagattt cccgtaaccc ctcatcgatc gtaaagatta cgtgccgatg tgtcacttta
     6361 aacatatcat tcgccacaac cgctgaccac ctttggcttt cacctgttgc acacgttgga
     6421 cagaactttc cacgacattt caagggaaca aactttgttt gatgacatcc ctcgcagaca
     6481 aacagtcggt atcctttttg aatatctcca caatatctaa acttgtttac ttcccgtttg
     6541 acgactagcc gaatctttcg cccgtatttc tcaacaaagt tatcccaatt atggtgttcg
     6601 tcaaaaaaga ttgcttcgag aatattcgct ttcatagtaa tattttactt gttcaaaaac
     6661 aaaatgaaaa gggctaaaag actggtagtg cagccttttt agcccttaac tttttatact
     6721 ttctttactg tc
//
LOCUS       DAYYHX010000149         6687 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000149 DAYYHX010000000
VERSION     DAYYHX010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.150"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..566
                     /locus_tag="ACHA6Y_002195"
     CDS             <1..566
                     /locus_tag="ACHA6Y_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8988271.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            967..1572
                     /gene="fic"
                     /locus_tag="ACHA6Y_002196"
     CDS             967..1572
                     /gene="fic"
                     /locus_tag="ACHA6Y_002196"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM8988272.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1588..2160
                     /locus_tag="ACHA6Y_002197"
     CDS             1588..2160
                     /locus_tag="ACHA6Y_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8988273.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2412..2669)
                     /locus_tag="ACHA6Y_002198"
     CDS             complement(2412..2669)
                     /locus_tag="ACHA6Y_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988274.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2795..3610)
                     /locus_tag="ACHA6Y_002199"
     CDS             complement(2795..3610)
                     /locus_tag="ACHA6Y_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8988275.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4127..5452
                     /locus_tag="ACHA6Y_002200"
     CDS             4127..5452
                     /locus_tag="ACHA6Y_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8988276.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5445..5795
                     /locus_tag="ACHA6Y_002201"
     CDS             5445..5795
                     /locus_tag="ACHA6Y_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988277.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5792..5962
                     /locus_tag="ACHA6Y_002202"
     CDS             5792..5962
                     /locus_tag="ACHA6Y_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988278.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
BASE COUNT         2291 a         1024 c         1254 g         2118 t
ORIGIN      
        1 attattttaa gtctgaaaag gatacatcgt ctctaacgtt aactttagag gaaggagttg
       61 aacctgacaa ggtgacctct aaagtgataa accaattgaa agaaaaaggt agtttgaaac
      121 atttggggga atatgaagtg ttagatacgg ctatgttaac gaaaggaatt ggtcaaatcc
      181 tcagtacgat cacttatttt ataacagctg tagcaggtat atctttattt atcgcagggg
      241 taggtgtaat gaacatgatg tatatatctg tatctgaacg aacaaaagag atcggtataa
      301 gaagagccct aggagctact cgaaaatcga ttatgctgca gttcctttta gaaggtttga
      361 ttttaactct ttcaggaggg ttaattggct atttattagg aatggctctt gcttatagtg
      421 ttggttcttt gataaaggta aacgtgtcac ttgatctatt tactatttta ctagcggttg
      481 gggtatcttc ggttattgga ctggtctttt ctgtaatgcc cgcttcagag gctgcaaaaa
      541 aagatttaat tgatattctt agataaaatc agaatgacaa gaatatttac gttctagatt
      601 ctaaaataaa acacaaagag accaaccagg caccctattg tttctttgta gtatagagat
      661 attctttggc ttgtaagaga tcaatagagt taggtgaccg gagttcgaaa taaaggaaat
      721 ttttgattgt ataggagaat tctttatccc taatatttag aattttgagt atgccatgtt
      781 gatgttttag attctctata aatatagagg tgtatcaaag gtcaactaga ctgtgcaaaa
      841 tagagacact tgaaaaatct atcgggggtc agaaacttaa tagtttttga cacataaaca
      901 aaatcgcgat ataatggggt tgagagtgtt tcataaactt atgtcataaa gttaaggaga
      961 tttcgcatga ttttagagaa taaattggga ttaaccaatc aagttgagtt ggctaaggtt
     1021 gaagaaaaac tcagtaaaca aaaagcgaaa gaattgtacg attgtggaaa aataaatgaa
     1081 atcgaagttg gtacatttaa aggtttatct gaaattcatg aatttctatt ttcggatatt
     1141 tacgattttg ccggaaaaat ccgatcggtc aatattgcaa aaggaaattt tcgatttgca
     1201 ccagttatgt atttagaaca ttcgttacag catattgatc agatgccaca aacttctttt
     1261 gaagagatta tcaagaaata tgtggagatg aatatcgctc acccatttcg agaaggaaat
     1321 ggccgaagta ctagaatttg gctggatctc attttaaagg aagaattgca aaaagttgta
     1381 gattggaact taatcgataa agcagactat ctttctgcaa tggagcgaag tcccataaac
     1441 gatttggaaa tacgctatct aatttccaat gctctgactg ataaaattga cgatcgcgag
     1501 ttatatatga aaggaatcga tgtcagctat ttctatgaag ggtattctga gtatacgata
     1561 gatgatttgt aaaagtaggt ggaccaaatg acgaaatatg gatatgcacg tgtctcaacc
     1621 gcttctcaaa gtacaaaaga acaaattcaa cagttgcttg agaacggcgt agagaagaag
     1681 aatatttttt cagaaaaatt tacgggtaca aaacgggatc ggccaaaatt caatcagctg
     1741 ttggctggtc tgaagcctgg agatgaaata gttgttacga agcttgatcg atttgctcgt
     1801 aacacgagag aagcattaga gatcattgaa cctttactcg atgataacgt aacgatcaag
     1861 gttttgaatc taggaacgat tgaaaatacg tccatggggc gaatggtgac acggacgttg
     1921 ttgagtgtag cagaaatgga acgtgatatg attgtggaac gtacccaaga aggaaaaatg
     1981 tttgcaagaa agaataatcc taatttcaga gaaggacgtc cgaaagcaac aatcacaccg
     2041 aagaagaggc acgcatatga actgcttact tcaggtaaat cgtataagga agttgaagct
     2101 ataaccggtt tcagtcgcag tacgcttttt agaataaaga agaaaattga agaaagttaa
     2161 aaaagaagtc tcagcgaacc agacaggtaa atacttaatg cgttctagat atgagtctat
     2221 tagcatgttg tgaaatataa tattttttta tttacttgat ttctgctcaa aaaatgtgta
     2281 tggtaactgt aaaaggtaaa gtaattaaag gtatgctagc cacgcttact atattaggat
     2341 accatcatat atttataatg aacgatttgt tcgtctacca aacaacacat atcgttcagc
     2401 gtaagtcacg tttaggaacg tggctttttt agtgttatta tcgataaata atctattgac
     2461 tctattgatt ttatctccat atccctacgg tgttgattgt ctttagatct ttcatttaag
     2521 aaccaaataa ctatcataaa aatcagcact gctgctaata taataaacag cattcctatt
     2581 gtgatattac ctattgattt taccataact acgattaaag taataatggc attcaagtct
     2641 cctttgttct ctatatataa attagccata aatcatcttc ctttcaaatt aagatacttg
     2701 tttgatacca ataaatagta agcgtggcta gcataccttt ttctgtaata gcaagtgttc
     2761 taatagtcca aacttaatga tggaccgatg ctagttatag tacagtaatg ttttctttct
     2821 tatctagatt tttagtaagc tctatcagca aatctaacaa atctgagtcc ttttcagaaa
     2881 ctaaattaaa tctagtgttt acaacaaagt tttcatgttc tatattgttt agattatttg
     2941 cggaaaggag ggcttctaga tcttgacatt caaatctata tgcatcgtca taaatcttac
     3001 tagaaggagt gcttaattcg aaattactta ttttctcatc ttggaccaag ataaatttat
     3061 agatagtatc tttttgtgtt tttcttttaa aaacacaaca gagtgtgtta taaggaacgt
     3121 tataattttc atcagcctca ttttttctcc attcaaacaa atgataaact gcaaccaagt
     3181 tgccatcaat ttcatggaca gtgctctcgg taacatcctt taaagaacta tcaagggcta
     3241 atttatagta actagagaga ggaagtaaaa ttgtcgattt actgagatgg ttatcgtcta
     3301 tacgtgcaat catacctaaa tcaactaagg ttttattcca ggtgtttata gttttagttg
     3361 tcactcctag agactctgct atggtattga ccgaatgcca ggaatccccg ccttcattct
     3421 ttgccttaaa acagtaatac agatataaat taatagcttt gttattcggt aaatattttt
     3481 caagttctgt tggtagaata aagtaagaat ccttaatgtt ttttctgaac tgtctccagt
     3541 ctctgtagtt ttgtttataa agttgtgaaa cggtttttcc acctttagaa agtcctgcta
     3601 tcttaaccaa tcttattgct cctttcttga atctgtcatg atcatacagt attttttact
     3661 tgtagtccag caccttaata tattatccta tgtaattaat ttcgtactag atagtaatta
     3721 ttttttattt aattctctag tcacttacaa ataagttata taaactacga ttaataaatt
     3781 attacaaaaa ctattaagaa aacttctaca tacaactatt acaaaactat taagaaactg
     3841 aatactccta ttttttgttt ttaaggaaag ttttttcttt tttgaatttc ttattttttt
     3901 ctaatataac taagcaaaac tggataatac gacacttatt ttcaaatttt cacaagacta
     3961 atagtaaata tccatttaat aaagtgatac aatgtgttga ttttacaaat caacagaaga
     4021 agtagggaaa taacttacct atgcttcttt ttttgtacat ttaatcgatt cttctttaca
     4081 aaaacgaacg tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc
     4141 aaaagagcca gtaaatgatt actttttgat cgatatgaaa agcttttacg caagtgtcga
     4201 atgtatagaa aggaatttag atccattaac gactgaatta gtcgtcatga gtcgagcgga
     4261 taatacagga tcagggttga ttttagcttc ttcaccagct gcaaagaaga agtacggtat
     4321 ttctaatgtc agtcgaccac gagatctacc gcaacctttt cctaaaacgt tacatgtcgt
     4381 tccaccacgc atgaatctct atattaagcg aaatatgcag gtaaacaata ttttcagaag
     4441 gtatgtagca gacgaagatt tattgattta ctcgatcgat gaatcgatct taaaagtgac
     4501 cagatcaatg aatcttttta cgactgaagg aacaagaagt caacggagaa agaagctggc
     4561 tcaaatgatc caggagaata ttaaagaaga gctaggattg cttgctacag taggtgtcgg
     4621 agataatccc ttattagcta aacttgcttt agacaatgaa gcaaaacata atgatggatt
     4681 ccttgctgaa tggacgtatc aaaatgttcc tgataaagta tggaatattc ctgaaatgac
     4741 agacctttgg ggtattggat ctcgaatgaa aaaaagactc aaccaaattg gtatcatgag
     4801 tattaaagac ttagctaatt ggaatccata cactattaag agccatttag gtgtgattgg
     4861 cttacagctg tattttcatg cgaacgggat cgatcgaaca gatatagcga tcccaccaga
     4921 accgacaaag gaaaaatcat atggtaattc acaagtgtta ccacgagatt ataccaggag
     4981 aaatgaaatc gaactggtgg taaaagaaat ggcggaacaa gtagctattc gtatcaggca
     5041 acataactgt aaaacaggtt gtgtccattt atacattggc acgtcgatct tagaaacgag
     5101 accaggtttt tcacaccaga tgaagatccc tataacagat aatacaaagg aattgcagaa
     5161 ttactgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat
     5221 aacctatagc aagcttgtgt acactgattc gcttcagctg gatttattta gtgatccaca
     5281 aaagcaaatt gacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg
     5341 ttttacgtcg attgtacatg cttcttctat gttagaaggc gctcgtagta ttactagaag
     5401 tacattggtt ggtggccatg caggcggcgc aggagggctt aaaaatgatt gattataatg
     5461 atcgtggacg tttaaagtgg gcaggttttt atttatctga tcatactgaa aaaattgatg
     5521 ccgacaatga acaacgagcc aatcaaaatt tggcaaaaga acaaatgacg actgaagagg
     5581 ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatgtatt caaaaagaag
     5641 aacgaaacgc tgaaggatac tatccagaag atattgttag caagatctta ggtagtgatg
     5701 agctggggat ctacgttgga aaaaacaaag ttcactacga cgaaatacgt catatcgaat
     5761 tttaccagga taataaatgg ggtcgattag aatgaacatt atccagcaat atgagctaaa
     5821 gtatataacc tttgatcagc tatcagaaga gatttggggt tatggccagc gtttgataaa
     5881 tgaggttggt gttgaacgct tctctttcta tgtagaagct gcagcaggat accataaata
     5941 cgaatactat attggctatt aaagtgcaat aaaattatat ttcttttatg ttttacattt
     6001 tgtatcaatt ctgataaaat tacctatagg taattaaaaa atataaaaaa actagttaca
     6061 accttaggac gttgtaacta gcttgaagca tactagagct aactaaaata gcactagaaa
     6121 gcaatgttaa acccgtccaa caaacttgat ttaacattga gaccagctta ttcagctaaa
     6181 tatagttgaa taagcttctt ttgttttaaa aaaacaaaag gtgtactacg aataacgcaa
     6241 gtaaaagaaa tactattaca accagtaggc tacaagtaag tattactaaa cattgcgtag
     6301 ttagaacctt tgctctattg tctaaagcca caaaattaga gatcagcttt atgatgctaa
     6361 gagcgagctg catccatgcg gacataggtt cttttgacac tattaattct cctttttagg
     6421 ataagggaaa ttgctagtag ttttttcatt agcaccatcc cccttcattt gattgagtct
     6481 agtgctgctg tgatagtttg ctgtttaata tgcttacatt cttattataa cataaatgaa
     6541 acaaacacat ggttagaggt cgtatcaatc aaaatagtgt ctcacaaacg atttattact
     6601 tactcctgct gactaacgga ctgttcttca gtaactgtaa ctatatgttt gttaaaacca
     6661 ccattacctg tagataaaaa ccaatat
//
LOCUS       DAYYHX010000150         6685 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000150 DAYYHX010000000
VERSION     DAYYHX010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.151"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..616
                     /locus_tag="ACHA6Y_002203"
     CDS             <1..616
                     /locus_tag="ACHA6Y_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8988279.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            613..1386
                     /locus_tag="ACHA6Y_002204"
     CDS             613..1386
                     /locus_tag="ACHA6Y_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8988280.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1383..1781
                     /locus_tag="ACHA6Y_002205"
     CDS             1383..1781
                     /locus_tag="ACHA6Y_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM8988281.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1966..2637
                     /locus_tag="ACHA6Y_002206"
     CDS             1966..2637
                     /locus_tag="ACHA6Y_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988282.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2696..3136
                     /locus_tag="ACHA6Y_002207"
     CDS             2696..3136
                     /locus_tag="ACHA6Y_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988283.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3148..3816
                     /locus_tag="ACHA6Y_002208"
     CDS             3148..3816
                     /locus_tag="ACHA6Y_002208"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8988284.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3880..4503
                     /locus_tag="ACHA6Y_002209"
     CDS             3880..4503
                     /locus_tag="ACHA6Y_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM8988285.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4559..4945
                     /locus_tag="ACHA6Y_002210"
     CDS             4559..4945
                     /locus_tag="ACHA6Y_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM8988286.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5158..6549
                     /locus_tag="ACHA6Y_002211"
     CDS             5158..6549
                     /locus_tag="ACHA6Y_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM8988287.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         2128 a         1081 c         1489 g         1987 t
ORIGIN      
        1 gtttgttttt gcaccgttag aaggagaaga cctcgcttca tttctggttg gtgcggcaat
       61 catgatgatc ggaatgacgc tctttttgtt tggagcagat tattctatga tggaagtcgg
      121 agatctagta gggaaatata tgatcaaaaa gaaaagtttg accgttttgg tcagtttggg
      181 atttgctatc gggatcgtga tcacgattgc tgaaccttcc gtgcaagttt tgggacaaca
      241 agtatatgat atatctgacg gagagattgg cagagtgttg ctgattggga tcgtcagcgt
      301 cggcactgga gtatttctag cctttgcgct gcttcgggtc gtattcaagc tatcctatta
      361 tcaattgatg gctataggct acataggcgt tttgatcgcc tcatttttca ccagttccga
      421 atttatgccg attgcttttg attcgggggg agtgactact ggtccagtaa ctgttccatt
      481 tatcttggcc cttgccggag ggatgactag tatgatcaaa caaaagaaaa atgaaaatga
      541 cagttttgga atggtcggta ttgcttcgtt agggccgatt cttgccgtga tgattttggg
      601 ggtgatcttc caatgagttt ctggcagcaa gtgatagatg gaatcgaaat cgttaccatg
      661 gaagtcattt tggcagtggc acctatcgta atcctatttg ttttaatgaa tatcattagt
      721 tttcgtatgc gcaaaaagcg tttctttgac attatgaaag gtttcttgat aacgactatt
      781 gggttgatcc ttttcctgca cggagtcaat atggcttatg taccggtagg acagcattta
      841 ggcagtgcta ttgcgggatt gccttacaat tatatactgg ttccccttgg ctttttaatg
      901 gggttcttag tagggtttgc tgagccggcg attcatgtaa tgatcaaaca ggttgaagaa
      961 ttgagcgagg ggagaatccg atcgaaaatc atgctgatgg taatttcaat tgggatcggc
     1021 gcagcagtag gattgtctat gtggcgtttg ctcgtcggat tctctcttta ttatttcttg
     1081 atcccaggat acgggctagc atttatttta ggacgaaaag tggacaagat ctttttagca
     1141 atggcttttg ataacggtgg agtagcaacg ggcccgatgt gttcgacatt tattttatca
     1201 atgagcgtgg caatcgctgc acaaattgaa ggaagagatc cgatcattga cgggtttggt
     1261 gtggttgcaa tgattgcatt gacaccaata ttatccactt tagtactagg atatatttat
     1321 aaacgaaaag atgaaaaaga cagaatcgaa aaagaaagat tgcaaaaatt gggggaaagc
     1381 aaatgaaaga tcttgtagca ttgaatcttc acatgattgt gacgatcgtt gacggaggaa
     1441 tgggcgaaga tgttatcgac ttttcaaaag aagcaggtgc agcaggcgga acgatcttac
     1501 atggaagagg ttctggtgta catgattcag ggaaattttt aggtttggac atttcgccag
     1561 aaaaagatat tgtgttgaca ttagtacccg atagtttgac gaataaagta atggatgcga
     1621 ttggtcaagg catcaacatc gatattccag gtaatggtat ttgttttagt attgatgtag
     1681 acaaagtcat tggtatcact aagattcatc aatatcgaga aattatcgga atgcaagaac
     1741 ttttagatga aaataacgat agagaaaagt ctgaatcata gacaagcgtt tgaagaggaa
     1801 ataaacacaa acaagaaggc ggatgagcgt tatttccgct tttttgtttg tatttttctc
     1861 gtgaatactt atgaaatagt aaatttttat gaaattctac tctaagcatc agactttctt
     1921 ttcaaaatat gctattttaa aaatgatgaa aaaaggggga gaaaaatgaa ttttttagtt
     1981 gttttaaaag atactctgat tgagcgattt acatttctat atgttcaatt gcaggaaaat
     2041 aaaacattgc tgcatttttc accagagttc catctagagg ggctaacatt aggagaaatc
     2101 tatcaagcag aagggctgtc ggctgttttg cactttttta aggtagaatt ggaattacca
     2161 gtaaaagatt atatcgagct ttctcttgaa tcttgggatc gagcagtggt tgaactgttt
     2221 ccagaaggtt taatgatcga tacaaacgat gggaagatcc atctgactgc agcagaactg
     2281 caaaaacaaa tgcgttatca aatagatgac gaaaattctt tttctatttt tagacgtcag
     2341 caaaagattc taaaaagctt tttaaaagtg atcagtaaaa aacggaatct gatcaaaacg
     2401 acacagctgt tgaaaaaata tccaggtctg cttcacacgt ctattcaatt tcctcaattg
     2461 attacgatga tccatcgttt tatacgtatg caacagctac catctaagaa gctgtttctt
     2521 ccattgacgg acacatttcg agtagtcaac agagaagata aaaagaaagt gattgtgatt
     2581 gactttacca gaaatcggaa tgtccttcac caagttgcaa tggaaaaggc caattaaggc
     2641 tttttctttt tggatttttt tgtcataata aatattagat gatggaggag ataatatgat
     2701 gcaaacatgg ttagaaaggc taatacgatt tcagcagaac tatcacaaac ttatgcaggg
     2761 aagatatgcg aaatttgatc agttgaatcg cggtctattg atcgctgcgc ttgtctttag
     2821 tttgctaagt cgttggttgc cctatagaat cggacaattg ttgttcatcg tctttttcgg
     2881 atggatgata tttcgtttcc tatcaaaaaa aatctacccc agactaaatg agaatcaacg
     2941 ttatttgaaa tatcttgata gagtcaaaga aaaatatcat cagataaaaa acaaaaagca
     3001 agtcaaaatg gatcgaaaaa gttatacttt ttttgagtgc ccgaattgtc atcaaaagca
     3061 gcgtgcacct aaaggaaaag gacgaatacg tgtgacatgc aaaacatgtg gtataaaatt
     3121 cgaaacgaat gtttaaggga gaaaagaatg aaattagtat tattacgtca tggcgaaagc
     3181 gaagcaaatt ttgaaaatta ctggacagga tggctagatg tcgccttaac agaaaaagga
     3241 aaagaacaag cagcagttgc cggtcaaaaa atgcttgaag ccggacttca attcgatgcc
     3301 gtttatacct ctgttttgaa acgtgcgatt cttaccggac aagtcgcact agaagaaatg
     3361 ggtcagttat ggcttcctat aataaaaaca tggcgtctaa atgaacggca ttatggggat
     3421 cttgtgggta aaaacaaaga taagatgaaa gaaatatacg gaaaagatca agtaaaaaca
     3481 tggcgcagag gatatacgga agttccgccg ttaaccgcag aaaatagatt tgaccgcaga
     3541 tatgatcact tggatccgca tttgatccca tacggagaaa gtctggaaat gacagtgaat
     3601 cggatcattc ccttatggca agatcatcta gcaccaaaac taaaagatcg gcaagattta
     3661 ctagttgtgg gtcatggtaa tagcatccga gcactgacaa aatatttaga agatgttcct
     3721 gaagatcaaa tggatacgat cgatatccca aatgcacaac caatccagta tacattagca
     3781 gatgatttga caattctgga caaaaaaata ctttaaagaa cacctttatc taaaagtgag
     3841 agatagaaaa aagaagcaaa ggaatggaac aaaaggaaca tgaaaaatta tatatgggac
     3901 tttgatggta cattgtttga tacgtatccc gcaatggtag atggtgcatg gcaagcacta
     3961 aaagattttg gtatcagtat ggacaaaaaa gaaatctact tcaaaatgaa aaagtattct
     4021 actagttatt tgatcaatga atcaaacttg aatgccagag agtttaatga attatttcac
     4081 cgatatgaaa aagaaagtac ggaagtttcc cgtccatttc cagaaacgaa acaagtactg
     4141 gaaatgctaa aagacaacgg cggtcgtcat ttcatcctga cccaccgttt gacagaatca
     4201 acatggggcc tgttaaaaga acatcgattg gcacatctga ttgaggaagt agtagggatc
     4261 gatcaggact ttcctcgtaa acctgatcca gcatctttaa attatttgat cgatactttt
     4321 catttagaaa gaacagacac gatgatgatc ggcgatcgtc gtttagatat cgaagcaggg
     4381 aaaaatgcag gagtcgctac ttgtctttac gacatcgatc attttctggg tgagatcccg
     4441 gcagattatg tggtaggaaa tctgaatgaa atccttacct tgcagagcat cattaaaaag
     4501 tagtatcccc tttcaaagga gatatgttat aattttcata aaggagtgat aaaattatat
     4561 ggaaatcaga agacaaaaag aagttgtaga agcatttcat tatgatatga gattgccgaa
     4621 ccaagaagta gaaacagatt tgaaagtagg cttctcgcct ttaaaagaaa cgatcgaaaa
     4681 ctatccgaaa gaacatagca tcgtaggagc acgcttagag ttccgtttag tgtttgatac
     4741 atatgtttta tctggaagtg tgagtcagat caatcatatc ctcaatcgga aaattgaagg
     4801 tcaaaatgat gtcacacaag aagaagtgga cgagctagta gcaccgttat tcgatatcgt
     4861 acgccgaatg tcttatgagg tgacagagat cgccttagat cagcctgggg taaaattgaa
     4921 ttttcaatca gaaaaacaag aatagacagt aaaaacatac ctgcgataga ttaaagctat
     4981 cacaggtatg tttttttatt actaattgta agcaaaaaga atgtcatttc gataaaaatc
     5041 gtctaaaata taaaagtcac tgaaaaaaat tttttcagga ggatggttga agaatcattc
     5101 tcgggaacct ttacttcctt cgatggactt gtcattaata atatgaggag gagaaaaatg
     5161 acaacactca tcacagtttt agtggttcta gtttttgcgg cattattata tgtgttctat
     5221 cgtatgcaaa agcgccatac aaaattttca acacgcgtct ttacagcatt aggagttggt
     5281 atcgtctttg gcggtctgct tcaattgatc tttggtgtag acgacaaagt cgttactcaa
     5341 tcaatggaat ggatcgggat tgtaggtcaa ggatatgtat cattcttgca aatgctagtt
     5401 atgcctttag tgtttatttc gatcattggt gcattcacaa aaatgaaagt ttctgaaaag
     5461 ctagggaaaa ttagtgcgac tgtattgact tctttacttg gaacaacagc tattgcagca
     5521 ttgatcggaa ttggttcaac aatgctgttc ggactccaag gagctaaatt cacacaagga
     5581 gctgcagaaa cagcacgaat ctctgaactg gcaacacgtc aggaaacagt tgaaaactta
     5641 tcgattccac agcagatttt atcctttatc cctaaaaacg tttttgctga tttagcaggt
     5701 agtcgtgcaa caagtacgat tggagtcgtt attttcgcgg catttgtcgg aatcgcttat
     5761 ctaggcgtga aaagaaaagc gccaaaagaa gcagcattct tttcagattt agtcgacagt
     5821 ctgtataaaa tcattatgcg gatcgtgacg cttgttttgc gtttgacacc ttatggagta
     5881 tttgctttga tgacaaacgt tttggcaaca agtgattttg cagctatcgt caatttaggt
     5941 aaatttgtcg ttgcttctta cgtagcattg cttgctgttt tgattgtcca ctcactgatt
     6001 ttgattggtg caaaagtcaa tccaatcaac tatttcaaaa aagcctttcc ggtattgagc
     6061 tttgcgttta cttcacgttc aagtgctgga gctttgccat tgaatatcga gactcagaca
     6121 aaagctttag gagtagatga tgcaacagct aatttctcag caagttttgg tttgtctatt
     6181 ggacaaaacg gctgtgctgg cgtttatcca gctatgttgg caacgatcgt cgcaccagca
     6241 gctggagtga atgtctttag tttggaattt attttgatgc ttgtggcaat tgtaacaatc
     6301 agttcttttg gtgtagctgg agtcggtggg ggcgcaacat ttgcttcact gatcgtttta
     6361 ggggcaatga acttgccagt agcaatcgtt gggttagtta tctctgttga accattaatc
     6421 gatatggcac gtacagcagt aaacgtcagc ggtagcatgg tagctggtat cgtgacaagt
     6481 gcacgcatca aagatttaga tcgcgatgtc atcaatgatg attctaaagt gatcgatgtg
     6541 aatgcatagt caaaaagagg ttgtgataga agtggtcagc tttaagaaat aagaagaagt
     6601 ttcctaaaaa tagtttctca aatttttgtg aaatttcggc ttatttccga gaagctactt
     6661 ctgaaacacc gtgaataaga ggttg
//
LOCUS       DAYYHX010000151         6567 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000151 DAYYHX010000000
VERSION     DAYYHX010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.152"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            280..516
                     /locus_tag="ACHA6Y_002212"
     CDS             280..516
                     /locus_tag="ACHA6Y_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988288.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            629..871
                     /locus_tag="ACHA6Y_002213"
                     /pseudo
     CDS             629..871
                     /locus_tag="ACHA6Y_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            961..1200
                     /locus_tag="ACHA6Y_002214"
     CDS             961..1200
                     /locus_tag="ACHA6Y_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988289.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            1347..5630
                     /locus_tag="ACHA6Y_002215"
     CDS             1347..5630
                     /locus_tag="ACHA6Y_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFM8988290.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            5729..6094
                     /locus_tag="ACHA6Y_002216"
     CDS             5729..6094
                     /locus_tag="ACHA6Y_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988291.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            6096..6548
                     /locus_tag="ACHA6Y_002217"
     CDS             6096..6548
                     /locus_tag="ACHA6Y_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988292.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
BASE COUNT         2349 a         1033 c         1434 g         1751 t
ORIGIN      
        1 caatttttgt atacaagttc tagtaaaagg atctgaagaa aaagttggtt gttgtgaaga
       61 taaaaaaaga atcaggtttt actgagtttt tcttcaaaat ttgtgttatt aggttaggac
      121 aaagataaga agaagtatga agatttaaaa aaggaatgag gaaaagatag taatttccga
      181 ttattggagt ttttttattt ataaagaaga agcgaataag ttgtgattcc tagatttcgc
      241 gcgtgattaa ttaatgagca aaaaatgcgg aaagttttag tgtttaatac acgaggacaa
      301 agtttcttag gtattgaagg cacacgtgta acggttgatg ggtggtgtga ttgtttcgag
      361 gttggaaaac atgttgaact tatggcattt actgagctaa aagataagaa caacgtggaa
      421 tttttcgaga gtgatattgg ttgggacgat catgaagaag tttatggaaa atttattttg
      481 cggaagggaa attcttatac gaatggggaa gcataagtag cgatttgtta gagatcattg
      541 ataggattga aattgtcggg aatatttatg agaatctaga gttatttgag gtgatagaat
      601 gagtgatggt tttgaacaag tatcaataat taaaaatcaa gttcgggaga ttcttagaaa
      661 aaaaggctat ttggtagata gttattttga aggagattac gaaacatggg taggtgtcta
      721 tgctcgtcca gagaataaac ctacctattt agatcctacg acgtcagaag atgcctattt
      781 acaaaatagg tatcgagtag atggttttaa gcaagacttt gcggagtggt ttgaatggga
      841 aattgaaaac ggagaagtaa aagaagaata agagtacttg ttgtatcaaa ataagccagt
      901 agaacaacca aaccagtagc tagtaatgac aataatactg aggaatcgga tcattatggt
      961 atgattgata tttctcttcc agaagatctt aagccagatc ctgtaacaat tggcaatagt
     1021 gtcatgattt ttgattcatt agatgaagct atgtcatatg gttggaaaga aattaaggat
     1081 gaaggtagta attggtatgg catgagattt ggtgatggtg aagttgccaa aaaagctggt
     1141 attccaattg ggaaatggac agttgatttc cacgaagcag gttctttttt tgttctgtaa
     1201 ttatagtggg agggtaaaat cattgatgaa atgtgttcag ataagagcgc cagataaaaa
     1261 tagaagagac agttcacgtc gttatcactt ttggtaacgg cgttttgttt tattatataa
     1321 aattctcagg aaaaggagga attcttgtgt atgcaaaaac gaaaatgaat tggttgaaag
     1381 cactcgcatt ggtggctttt tctcaattct gtttcttggt gggaactgct caagcacatg
     1441 cagaagaaac gacggctata caacctacga tggaagccat tgcccaagtc gcttctgaag
     1501 tgacagaagt ccaaccagca gaagccgggg tcccagctgg tgaagccact gttgaacagg
     1561 cccctgtgac agaagcgcca acggtcaatt ccaaaacgac tctcgatcag acgattgggg
     1621 aaacacaaca cccagggaat agtcctggta cttcacaacc agttgaagaa actgcctcaa
     1681 ttacaacccc tgcttcagaa acgtccactc tagaaggaga aaaagctgcc gaagcaacgc
     1741 cttctgtgca gccagagtct tctgagaaac aagcggaaga aaagaaacga atagaagaaa
     1801 cagtggaaca accagcggct actgctgaca aagaaggaaa aaatgaggaa aacgttgaaa
     1861 aaacaatctc ttcaaccgca gctagtcaaa atcaaccaac gaaaccaaac aaagaatcta
     1921 cggctagtat tgaaaatcaa aagaaaaaag agcagaacat tccaaaaaca ggtacaccta
     1981 cactgttagt aacagtacca accaatcgtt cggttcgtgt tcgccgtgca accaatgcag
     2041 ctgttttagg tggtcggaaa attgattggg gcgaaaatat tggtgttact tctagtccga
     2101 acaatctaaa cttcacttta aattatatgg ttcgttttga gattaatgga caagtggcgt
     2161 tttgtattga gcctggtgtc tcagccgcag aaggtcaagt ttatacagct actgctttga
     2221 aaaactattt gaaaaattca aatgtacgga aaaaaacaag cttgatctct tatttaggat
     2281 atgtcacaaa taaagacaaa tccgatgaac aatacattgc ggcacaattt atgatttggg
     2341 aagctttggg cacaaaaatc ccaatcaaaa atatccgtgt tgattatgat cgtcgaaaag
     2401 cagaaattca acgtttgatt aataactttg gtgaaaaagc aacttttaat aatcaaacac
     2461 atacggtaaa aattggcgaa acgttgaaag taaagaataa aaatgatttt ttgaaatatg
     2521 tgaaagacat tcaaaaacca aaaggtgtca atgtatctat tcacggcaat gaagtatgga
     2581 ttacagtcac aaaagaggca ccggataaag caaaaattca attgaataaa attaaagttc
     2641 aaggtgcgcc attagcctat cgtcgtccag gagctcaaac aattggtgtg ttacatccaa
     2701 cagaacctaa tgcgtctgtt attaatttac aaattctaaa acaaggaaac attcgtgtgt
     2761 ttaaagaaga tgcggaaact ggtgtgaaag cacaaggtgc agcttcttta gaaaatgcgg
     2821 tctatggttt gtatcaagca gatgggaaaa agattaaaga aattaccttg aaaaaaatta
     2881 acggcaaagt acaggcagaa attaaagatc ttgatcctgg caaatatgtt ttaaaagaaa
     2941 tcaaagcgcc aaaaggttat gtgctttctg atgaagcaat tgaaattgtt gttgaacctg
     3001 gaaaaactgt caatgcgacc gcaaaagatc aagtgatcaa aggaaccatt gatttagtga
     3061 aagttgctaa taaagattta gtcgattcaa cgaatccaga taacaaacca aaattagctg
     3121 gcattgaaat cactttgacg tcaaaaacaa ctggcaaagt ggtgaaaacc gttgtgacag
     3181 acaatgatgg ttatgctact tttggtaaaa ataccgttgt ctttgatacg tacattcttt
     3241 ctgaaaccaa aggaaaagaa gggtataaac tatttgaacc acttgaagtc acaatttctg
     3301 aaaatgggca aactttccat tatgttttag aagataaagt aattgaacaa cgtcttaaag
     3361 tggtgaaagt ggatcaagaa acaggaaaag tgattccact tgctaataca gaattcaaga
     3421 tttttgatac tttagccaat cgttatgtca caatggagat tccaaatgat acagaaacaa
     3481 cggatatctt taaaaccaat gaaaaaggct ttttcctaac taacggtaca cttacttatg
     3541 gaaaagatcg ctatcgtttg gaagaaatca aagcccccgt aaattacgtt ttgaatcaaa
     3601 acccattagt gttctcgatt accaatgata cagaaccgat caaagtgatc cattttgcga
     3661 accgtcaagc acggaaaagt gtaaaattat tcaagtatga agaatggaac aagaagaaaa
     3721 cgccgctcgc aggtgtgaac tttaccttgt ataacggtaa aaatcaagca attggtgaat
     3781 ataccactga tcaaaacggg gaaattttag tgaaaggtct agttgccgat gactattact
     3841 tcttggaaaa tgccccatta gaaagtttcc aaccaaatac agataaacaa gcattcacgg
     3901 ttgcattcaa acaaaatgcg ccagatgatg gggaaacgtt agtggtttct gtacgtaatt
     3961 acttgattcc accaaaacta gaaacacaag cgtcaaataa agccgatggt gaaaaagtgg
     4021 ttgatccatt agaaacgatc gtcattaaag acagtgtttc ttataccaac ttgtttgtcg
     4081 gaaaagaata caccatttca ggagtattga tgaacaaggc aacgaatgcg ccaattttac
     4141 aagatggcaa agaaatcaca agtaccttgg cctttattgc agacaaagtc aatggcacaa
     4201 aagaccttga atttgtggtt aatgctagtg ttctgaaagg tcaaacgatc gttgtttttg
     4261 aaaaactttt ccgtgatggc attgaagttg cagcacatgc ggatatcaac gatgaagccc
     4321 aaagtgtgaa agtgaataat ccaaccgttc ataccacggc tgtctttgaa gacggattga
     4381 cagtcgcaga cccaacagga ttgtttacct taaacgatac cgttgaaatt accgagattc
     4441 ttccaggaaa agcatacttg gtcaaaggga tcttaatgga taaagcaacc ggtgaaccct
     4501 tgcttgtgca tggaaatacg gtagaaacga gtgttggctt tattgccaaa gcagaaaatg
     4561 aaacggtgat cgtaccattt acctttgatt tgacaggtat aaatggtcgt gaaatcgtcg
     4621 tgtttgaaag tctttttaga aatgatgaag aactagcaaa ccataaagac atcaacgaca
     4681 aagggcaaac ggtacgagta actaacccaa gtgtacgtac aacggctaca cataaaccca
     4741 ctggcttgaa agaggttaat ccattaacga aagtcaccat tacagacgct gtgaaatacc
     4801 acgatttggt cgttggaaaa acatacacag tcaacggtgt tctaatggac aaagtaacgg
     4861 aaaaaccagt gctagtagat ggaaaagaag tcacaagtac cttaacgttt gtggcagaaa
     4921 caacggatgg tacagtcaat ctagacttta ttctagacgc acgtgcgttg cgaggcaaag
     4981 aagtcgtggt ctttgaagat ctgtttagag aaggcgtatt attggcttct cacgcagata
     5041 ttaccgacaa tgaccaaacc atcaaaatca gtaatccaac cattacgacg aaagcgacga
     5101 ataaaaatgg cggaaaagag atcttggcct tgccaaatca aactgtgctt gaatgggtca
     5161 aagtagacgg tcttgcgatt ggtcaaatct atcgcttaat tgttcaaggc tacttaacac
     5221 cagacggcac accatttgaa ggcactcatg cggaaaaagt ctttgcggcc gataaagaaa
     5281 tgatggaatt cttatttgaa ttcttagtgg acggtcgcaa tcttgcagga aaaggcttga
     5341 gttttgcgga atggctccaa gcgaaaacgg aagatgagaa agaaacgttt gaagaagtgg
     5401 cgaaacacaa tgtagatcta acaaacaaag atcaaacggt tcaaattgtg gaagcgccaa
     5461 aaccaccgca accagtgaaa ccagaacaac gggttaaacc agctactgtt gtaccaatgt
     5521 tgccaaaaac aggtgaacaa acaagtagta tacttatggt tcttggatta gtagtattag
     5581 tcattgcttt aggtataaca ctttataaag tacgtaaagc caaagattag tcatgaggaa
     5641 caagcggtta ggatttttaa ccgcttgttt gattgtaatg tagaaatatt tagaatttat
     5701 tttggttttt aagaggagga aaaactttat ggcagaaaaa gcattgcgta tgaagaaaga
     5761 gtttttaggt caagtatttt tattgggttc acgggaacct aatccaccaa aggaaattaa
     5821 tggtgtgtct gggcgatatg ttcaattaca agcagagtgt tcgcgagaag aacagcctgc
     5881 tttttttgtt tatctcacag ctgaagagga taaaagatta cgattaccag aaaaagaggt
     5941 gcgtatttca tttgaagaag cttattttgt ttttcgtaaa gaacgagtga atagtcgtga
     6001 aacaaatgat atgggacaaa cagagcgtcg aacaactggc tatcaagatg ttggtgtctt
     6061 tttaactggt gtaaaaattg gggaggtttt gtaagatggg attaaaattt ccaaataatt
     6121 atgacattac agaagcatta gatgaaactt gtttaggagc cgtacgcttt ggcgcattag
     6181 aagagtctga tcgtgtattt gataatgaaa caggcgatta tacgggagaa attcgatcac
     6241 agcgagcaca attagttttt ggaaatccag atgaaccaaa gattgcacga cgagttaaat
     6301 ttccagcaga tgtagatttg actggtttta aacgacgtga tcctgtgaaa ttaattgatc
     6361 cagaatatca tgtggtatat attccagcac aagatggcaa tcgtgaatat tcagatttag
     6421 tgatttatgc gaaaggatta gagaaagtag aagctccaac aaataatcct aatatgaaac
     6481 aatcacaaga aaaaggttta gatcaaaaac aaccaagtgt tgattctaag gaaaaagaga
     6541 aaaaataagg tttttttgaa agaatag
//
LOCUS       DAYYHX010000152         6491 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000152 DAYYHX010000000
VERSION     DAYYHX010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.153"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            480..1448
                     /locus_tag="ACHA6Y_002218"
     CDS             480..1448
                     /locus_tag="ACHA6Y_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8988293.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            1466..2371
                     /locus_tag="ACHA6Y_002219"
     CDS             1466..2371
                     /locus_tag="ACHA6Y_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8988294.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            2368..3174
                     /locus_tag="ACHA6Y_002220"
     CDS             2368..3174
                     /locus_tag="ACHA6Y_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8988295.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            3331..4299
                     /locus_tag="ACHA6Y_002221"
     CDS             3331..4299
                     /locus_tag="ACHA6Y_002221"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM8988296.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(4616..5140)
                     /locus_tag="ACHA6Y_002222"
     CDS             complement(4616..5140)
                     /locus_tag="ACHA6Y_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM8988297.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            5427..>6491
                     /gene="scm"
                     /locus_tag="ACHA6Y_002223"
     CDS             5427..>6491
                     /gene="scm"
                     /locus_tag="ACHA6Y_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8988298.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         2047 a         1322 c         1271 g         1851 t
ORIGIN      
        1 tactacactt attttctata aaagtcaaca cttcctggcg tttcttctgc tttttcgcaa
       61 cattttctta atgttttcaa ttttatgttt tcaattatct gacaattctt aattgacaga
      121 cctttttatc tcggttatac tttagaaaat tcattttgtg gaggaacttc tatgcgaacc
      181 aacatcaacc aaatcgcacc aacacaactg aataactttt actttagcta ctttagatag
      241 gtttgtattc atcatgatgg atacaaacgt taaacgacag tttgtatcta tgatggctaa
      301 agaactttgt attgcagcaa aaagccacgt agacaaaatg ctaagtggct gagcagtgaa
      361 aatacaaagt aaaagtatca agcattctac tttttcaatt tattttccga cttttcaatc
      421 catttggaca ttttgtctga atggattttt tgtttcccaa aattaaggag gaaaaagaaa
      481 tgaaaaaaaa tataatcatc ggattaggcg ctgccctgct actagcttta ggaggttgcg
      541 cacaaaaaga caattccgca gaggcatcag atggacaaaa aacaattggc gtattgcaac
      601 ttgtacaaca taactcgtta gatgctgcct ataaaggatt caaagaagga ttagcagaag
      661 aagggtataa agaaggcgat aatctaacaa tcgattatca aaatgcccaa aacaatcaag
      721 acaacctgaa aagtatgagt gagaaactcg tcaaagaaga tcctgatttg ctacttggaa
      781 tcgccactcc agcagcacaa tctttattaa atgaaacaac cactatcccg atgactgtca
      841 cagcggtcac agacttggaa caagcccatt tagtcacatc caatgaaaaa cctggcggca
      901 atgtgactgg cacgacagat atggtgccaa tcgaaaaaca aatcggctta ctgttaaata
      961 tcgtgccaga cgccaaaaca atcggcatca tgtataacaa cggtgaagca aactctaaga
     1021 tccaagctga tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc aaagtactga
     1081 cagcaaattc cacaaatgat gtccaacaag tgacgaaaag tctagctaaa gatgtagatg
     1141 gaatctatat tcctactgat aacacgttcg caagtgcggc aagtgttgtc ggtgaagtcg
     1201 caaaagaaac aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa gacggcggtc
     1261 tagcaacagt aggaatcgac tacgaaagct tgggcaaaca aacagggatc atggcagcaa
     1321 aaatcttaga cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat gatttggaac
     1381 tgtatgtcaa taaagacatg gccaaagcat taggcatcaa tccagacagc atcaaagaag
     1441 ctgaataaaa cacaggagga ttctcatgac aaatttgctc tctcttttct tatcatctgc
     1501 atcgcaaggg gtcttatggg ccctacttgc gattggtgta tacgtcactt tccgtatctt
     1561 ggacattgcc gacttgactg ctgaaggaag ctttccttta ggtgcaggga tcgcggcagt
     1621 aagtattaca aatggctatc atccattgac agcttgtctt ctaggctttt tagggagtgc
     1681 ggcagcaggg cttgtttccg gactgcttca cacaaaattg aaaattcccg cccttcttgc
     1741 agggatcatc accatgactg gtttatattc tgtcacatcc cgtgtgatgg gtgcgccgaa
     1801 catctcttta ttgggtcagc caacgatctt tcattgggtc gagtcattcg gcataacaaa
     1861 agacaacgct gtactgatcg tcggtctgct ttttgctttg atcgttgtat gtttacttgt
     1921 tttgttctta cgtacagaaa caggacttgc tattcgtgca actggcgata atctggcaat
     1981 gagcgaagca aacgggatca acacagataa tatgaagatc atcggttata tgatttctaa
     2041 tggatgcatt gctctttctg gctccttgct tgcgcaaaac aacggctttg ctgatttaaa
     2101 ttctggtatt ggaacgatcg taattggact tgcttctatt atcatcgcag aagttttgtt
     2161 acgtaaccag ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg tcatttatcg
     2221 ttttatttta gcgttagtct tcgaactgaa tgttgaacca agtgattcga aacttgcttc
     2281 tgctttcgtg ctagtgatct gtctttcttt cccgcagatc cagcaaaaat tccatttacc
     2341 tcaaaaaaag aaaggcggta gtgtcaaatg aatgacgtct taacattgga aaatctccat
     2401 catacctttg aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat taatctttcc
     2461 ttagatcccg gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa atctactttg
     2521 ctgaacagca tcgccggtac attttctgtc gatcagggtc aaattttatt gaatggaaaa
     2581 aatatcacga aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt attccaagat
     2641 cctaaactag ggacagccgt tcgtttaacg gtagaagaaa atcttgctct tgctatgaaa
     2701 cgaggaaaaa aacgcggctt cttccgtggt gtcaaaccac aagaccgaac atttttcaaa
     2761 gaccagctgg ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga aatcggtttg
     2821 ctgtctggag gacaacgtca agctattacg ttattgatgg ccacattaca gcgtccagaa
     2881 ctgatcttat tagatgaaca cactgcagcg cttgaccctg ctacttccaa aacggtgatg
     2941 gaattgacag aaaagctgat cactgagcag aatctcactg cttttatggt cactcatgac
     3001 atggaagacg cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg aaggatcgtc
     3061 gtagatatcc agcaagagga aaaacagcag ctcacagtgc cccaattatt agcgttgttc
     3121 aaacaaaaca gcggcacggc attaaaagac gatcaagtac ttttatcttc ataatcagaa
     3181 agaggatggg acaaaaaatg aacgcccacc ctcttctttt ttctctttcc ttctttcgtt
     3241 tttgctttat gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc cttcttcctg
     3301 atagaatgga tgggaaagga gcttgatgcc atgacaacga aagacaaggt acttcaatta
     3361 ttgaaaaaga caccagattt tctttccgga gaaaagttgg cacagcaact ggaagtttcc
     3421 cggacaagta tttggaaagc catcaaagaa ttggaaaagg caggctatcg ttttgagcac
     3481 gggcctacag ggtatcgtta cctgccctct gacgtactag atgcaaaaga aatccagcaa
     3541 gagctacggt cgtttattcc tgatttgaaa gtacaaacga tgatgacttc tgaatctacg
     3601 atgaaagatg ccaaactggc agcagctcaa ggagattctg tttcgaagtt atttgttgct
     3661 gatacacaag aacaagctcg tggacgtttt ggacggcctt ttttcgctga acctggacga
     3721 ggaatctaca tgagtctctt gctccatcca gacaaacggt ttgatgaatt gcctcaatat
     3781 acgatcatcg cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg tcgttctgct
     3841 tcgatcaaat gggtcaatga tatctacatg gatggaaaaa aagtctgtgg gattttatct
     3901 gaagcagtca gtgatatgga aagcggcagg atcagtcata tcatcattgg gatcgggctt
     3961 aatttttctg ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc catctctgtc
     4021 tttcccgacg gacaagctag tgtcacaagg aatcagctaa tcagcaaaat ctggcaagaa
     4081 ttgttcactt atctttctca cccagaaaaa gcgttggact tctataaaga aaaatcattc
     4141 gtattgggaa aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg tcttgcaaaa
     4201 gaaatcggtg atcatggcga actgatcgtc caaacaaatg aacagaagat gagtttgtct
     4261 tctggcgaaa tcagtctcac atctatccgt acgcaataat tatcagattt cacaaaagga
     4321 cacctgatct catacagtgt ccttttttct gaattctctt caaaagactc tccttattct
     4381 atcagctgac agtttctcta tcctctagaa aaaggtattc taaaaatatc tataaagcat
     4441 ggtacacttt ctttaaatga atcaatgcaa aaaacaagag agtcatcagt ttacacgatc
     4501 aaaagagata gcaggccgct tcctttttca atcttataga tagaccatgg gtaacgataa
     4561 aaacaaaaaa gaacttgcga cttattttgc cgtaagttct tttttagcta gtgccttaat
     4621 ctcttaatcc caacaaacgt ctcgcataga tcacctgacc agagtgcata gcagcatcat
     4681 caataataga aacgagacgc attcctcgtg tgactggtgg tgtccagttc tcgtcaatta
     4741 cttcatctag ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa tcagcagctc
     4801 ctcgcaagta tcctagcaga tcttctattt gattagcttg gatctgcttt gcttgttcca
     4861 gactatgctt ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg ctttcccagt
     4921 cttgactatg ccaaattgga gaagttcctg ctaaatcagc aatctggaaa tctaattctc
     4981 gagcggtatg ccaagccagc caagtcattg atttgatctg cggggctcta tcttccactg
     5041 gaaaacgatt ggcctcctca atagagacgc cttcaaacaa acgttcaaac cgatcgtatg
     5101 cccgatccac actctcgcat agcatatcta aataatccat ttattttcct cctttttatt
     5161 tatcctactt actttttcct aattctagga tacctactta tatgttacta cacaccctta
     5221 ttttttacac tttaacagtc accatcatca atagtttgga gattgaaaga aaaagatagt
     5281 gtcgaactat caattgaaca agaagcaaca atatgaacgt ttcattaata ttctttaaat
     5341 aaacaatgat ttttcaaacg aacagtttaa gactaggaga aaaagagtat aataggaaaa
     5401 tgtttgaaag gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta gtcctagttg
     5461 ctttgttgtt caatatcttt tcgtatggtg tggccgtttc agcggaaaca tacattccta
     5521 aagaccctgg gaatatttat aatgatctaa ctggtaacta tggcttgtta ggaattgcta
     5581 gtcaattcca cgtatttgct aaaaataaga caactgtgaa tgcacataca gacggaaata
     5641 tagcaacaca tgaacttgat gcaaaaaata attttggtac agagcttcat gaaggtttat
     5701 tgaaccaaga aataaattat atacaaaaaa ctgattcttt cgctaattct tcaggcatac
     5761 ctacaggtag cacaagaatg aataaatttg tcgttggcag taacgtggat gtagcacttc
     5821 aagatggtca tcctacagtc aatggatcca ggcttgatca cttaactcca gatgattttt
     5881 ataaagatcg tgctgggcat gaatatattg atttcgagca ggaatttgct aaattgaatg
     5941 tagcagcaaa tgatcttgca acgatcactc cagctaaaac atatactgct gcagattttc
     6001 cagacatgaa caatcggaca atcgatttaa ctgatttatc agataccggg tttttattgg
     6061 taaatatcga tgcagaagtg ttaacgatga atacgccact tcaaatcatt aatccaaatg
     6121 atcaagtagt tgtatttaat gtcatcaaca gttcttctgc attgaacgta cagtcaccaa
     6181 tcaaatacaa tgatcgttca aatcatgaaa cagaagattt ttcggatgcg aatatctctt
     6241 ggaattttgg aaatgagatg actgatttga caatcagtgc tccttttcaa ggaacaatcc
     6301 ttgcgcctaa tgctactatc cgcattactc aaaaccaaga cggaacgatt ataggaacaa
     6361 atgtgatttt agatgcagct accaatcgtt gggatcctaa cgaaatattt atcagcgaca
     6421 atggtacaga tacgacagac acaagcgaca gtacagatac atctaccaca agtgatagct
     6481 cagattcatc a
//
LOCUS       DAYYHX010000153         6478 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000153 DAYYHX010000000
VERSION     DAYYHX010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.154"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..961
                     /locus_tag="ACHA6Y_002224"
     CDS             80..961
                     /locus_tag="ACHA6Y_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8988299.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            974..2584
                     /locus_tag="ACHA6Y_002225"
     CDS             974..2584
                     /locus_tag="ACHA6Y_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8988300.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2661..3320)
                     /locus_tag="ACHA6Y_002226"
     CDS             complement(2661..3320)
                     /locus_tag="ACHA6Y_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8988301.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3603..4268)
                     /locus_tag="ACHA6Y_002227"
     CDS             complement(3603..4268)
                     /locus_tag="ACHA6Y_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM8988302.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4415..5020)
                     /gene="dhaL"
                     /locus_tag="ACHA6Y_002228"
     CDS             complement(4415..5020)
                     /gene="dhaL"
                     /locus_tag="ACHA6Y_002228"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM8988303.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5048..6058)
                     /gene="dhaK"
                     /locus_tag="ACHA6Y_002229"
     CDS             complement(5048..6058)
                     /gene="dhaK"
                     /locus_tag="ACHA6Y_002229"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM8988304.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6033..6428)
                     /gene="dhaM"
                     /locus_tag="ACHA6Y_002230"
     CDS             complement(6033..6428)
                     /gene="dhaM"
                     /locus_tag="ACHA6Y_002230"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM8988305.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
BASE COUNT         1690 a         1385 c         1221 g         2182 t
ORIGIN      
        1 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
       61 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
      121 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
      181 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
      241 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
      301 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
      361 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
      421 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
      481 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
      541 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
      601 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
      661 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca ctcgatgaag aaggaacact
      721 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
      781 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
      841 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
      901 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
      961 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
     1021 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
     1081 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
     1141 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
     1201 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
     1261 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
     1321 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
     1381 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
     1441 agcggattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
     1501 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
     1561 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
     1621 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
     1681 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
     1741 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
     1801 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
     1861 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
     1921 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
     1981 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtag tcttgatttg
     2041 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
     2101 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
     2161 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
     2221 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
     2281 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
     2341 atggcttcct cgaatcggta aacttgctta tatctactta gcttactcct ttgcactcaa
     2401 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
     2461 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
     2521 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
     2581 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
     2641 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
     2701 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
     2761 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
     2821 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
     2881 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
     2941 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
     3001 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
     3061 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
     3121 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
     3181 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
     3241 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
     3301 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
     3361 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
     3421 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
     3481 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
     3541 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
     3601 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
     3661 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
     3721 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
     3781 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
     3841 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
     3901 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
     3961 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
     4021 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
     4081 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
     4141 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
     4201 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
     4261 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
     4321 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
     4381 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
     4441 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
     4501 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
     4561 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
     4621 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
     4681 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
     4741 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
     4801 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
     4861 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
     4921 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
     4981 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
     5041 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
     5101 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
     5161 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
     5221 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
     5281 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
     5341 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
     5401 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
     5461 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
     5521 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
     5581 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
     5641 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
     5701 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatcggctt
     5761 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
     5821 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
     5881 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
     5941 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
     6001 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
     6061 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
     6121 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
     6181 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
     6241 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
     6301 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
     6361 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
     6421 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttca
//
LOCUS       DAYYHX010000154         6402 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000154 DAYYHX010000000
VERSION     DAYYHX010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.155"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..880)
                     /gene="ispE"
                     /locus_tag="ACHA6Y_002231"
     CDS             complement(29..880)
                     /gene="ispE"
                     /locus_tag="ACHA6Y_002231"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM8988306.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(1034..3607)
                     /gene="mprF"
                     /locus_tag="ACHA6Y_002232"
     CDS             complement(1034..3607)
                     /gene="mprF"
                     /locus_tag="ACHA6Y_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8988307.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHLTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            3748..4554
                     /gene="proC"
                     /locus_tag="ACHA6Y_002233"
     CDS             3748..4554
                     /gene="proC"
                     /locus_tag="ACHA6Y_002233"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM8988308.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(4633..6189)
                     /gene="rny"
                     /locus_tag="ACHA6Y_002234"
     CDS             complement(4633..6189)
                     /gene="rny"
                     /locus_tag="ACHA6Y_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM8988309.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         1882 a         1421 c         1111 g         1988 t
ORIGIN      
        1 tttcatcacc tattttactt cttatatttt attgcagggt tcgtactaaa taaacttcct
       61 cacaaaaacc ttttaaccca ttatatactc tctgcgctct tgaataatgt tgacacaagg
      121 caaaaacggt tggtccgctt ccactcatca atgcagcatc tgctccgtac ttgatcatac
      181 gctctttgat ttgatggatg actggatgcc gctttcccgt tatatcttct aaactgtttc
      241 ccattttttt gatcatctgc tgataatctt gctctcggat ggcttcagca agtccgtgga
      301 tgtcttgatg ttggatggct tgcgtatcta cttctctaaa cactgtacgt gtagagacac
      361 tcattcttgg tttgaccaac acaacccagc actgtggcat agaaggtaat acttggattt
      421 cttccccttt gccaccgaca aatgccgtac ttccgtagac acagtaagga acatctgtac
      481 cgacttccaa accgatttct gccagttctt ctaacgaaca gcccagattc cataattggt
      541 tgatccctct tagagcagct gcacagtctg tacttcctcc gcctaatcca gctgctactg
      601 gaatattctt cttaacaaag atcttgacac cggttttgat attgtattta ttttttagca
      661 ataaagcagc ttgataaaca ttgttccgtt catctaccgg taaaaatgct ttatttgttt
      721 ctacaatgat tttgtcttct gttatttctt caaaaaatag atgatctgcc aagtctacgc
      781 tcgacatcac catttccaac tcatgatacc catcttgccg tctatataat acatccagtc
      841 ctaaatttat cttggctggc gctttttcaa tcaattccat cctctcaccc attcgaaaag
      901 tctaagtcgc aagtattcta tcatagggaa aaataaaagt atagcttatg tctgcctagt
      961 ggttgatttt gtcatcgttt caacagcttt cacagaattt tctcttcata atccaaacaa
     1021 gttctagtga ttcttataat tctgtgagca cttcttctgc caaactcgga acatttcctt
     1081 tttcttttcc tatcagactg agtagctgca tggtagcaaa tccgaaatta cttcctttcc
     1141 tataagcaaa ataacgtcct tcccaatgat cgacatattt taatttcttt tcataagcag
     1201 actggaagga atagaaaaaa ttaccataat tatatacgat atgcatgact ttttcttgaa
     1261 agaaagaaaa atctgtttct ccaacattag ctaatggagc taagcctaga ttggcttccg
     1321 agtagcccat tttctcgtat acaataaaca gattggctaa cagatagtca gctaattcct
     1381 cagattcttt aggtaagacc cgcaacaaat catatcccgc ctgcttcttc gtcatcggct
     1441 gagcggtgat gaagccaacg actttttgat ctttacggac aataccgaca ccgctagata
     1501 atacatatga tttatcaaat cgtcccacag agaagtattt ttctcgtgca ccttgaagcc
     1561 atttttgtga aacttcattt acagcttgat gtaattcttc cggcagttga tcgtaccaag
     1621 taaaatggta tccttctttc atcaaacgtt gatattcaag ttgctgactc agccgagaca
     1681 agtcttctgt tgctagatca acaatccctg cttcacctac tttcgtgagg tgaaaaccca
     1741 attcatgaag catcactacg tagctttctg atatcttata aaacgcaagc tgatagccta
     1801 aacaatctgc ttgtttcata aactgattcg ttgctgcccg catcatttta ggattgccaa
     1861 ctggatctcc taaaacaaag catttgtttc ctttgatctg ataaccaaaa acgacttgat
     1921 cttggtcgtc ttcttggtaa taaaaataac tgtaacctga caagcgaagc tgatggcttg
     1981 tagctgtgcc tccgtatgtt tccaataaac ggtcaaaacg gtctttttcg tactcactgc
     2041 caagcttcgt agtatcttca gctaaatatt gataaagcgc cactaaagct agcaatgaga
     2101 tgcccaaacc aataagccct gagaaccaca catcatctga aggaaacaaa ataaaatgat
     2161 ttcctgttat tcgaccgact aaaaagcgtc cgctgtgata gcctgcaatt gcgtaaacga
     2221 taaacaaaaa gccgaacaag ctagcatcaa agattatagc tccccaagaa taaacgaatt
     2281 ttgttcgata aaattctttt ctggataacc acacagctaa cagaatcaag acatatacca
     2341 atatcaactg ccaagataac gtacgtgtga ttgtatttaa aataccaaaa gatagtaaag
     2401 caatcgtagg cagaaatgcc tttttgactt ttactgaaag tgctctagca aggccgagta
     2461 ataaaaagcc aaccaataga ttcactgtct gatctaaaaa attgaatgag aacggcaata
     2521 atacttgaaa tagccgactg attgcagaca gattagtgat tgttgataga agtaccatca
     2581 tgatccccgc aaaatagaca gcacttacga gcacgatatg cgcaaccttt tggaaaaaca
     2641 gtcgcggaat gttatctaaa aatcgattga ttttgactcc tgtatgtgtg atgaacaata
     2701 gaagtcccgt cataaaaggt agaacataat aaaacaaccg ataatataat agccaaacta
     2761 ctgctacatc ttgccgaaca ggcaattgac tcattcctaa gatcatcaaa acgtcaaaag
     2821 tccccattcc acctgggacc atcgtcagca ttcctatcaa tgtagcaatc atgaacatag
     2881 gatagatcga aagcatcgaa atatctattc tcatcaactt gccgatgatc aaaaataccg
     2941 caagggctcc cgtccattgt ccaaaggatg ccagtagcag accaagcaag ccttttggat
     3001 aaaactcgaa gaacaatgtc tttctcttta gataagcaaa aaagaaaatg actggtgcta
     3061 aaaaactacc gccgaaaagc cagatccaat agcttcgaaa aatactttct gactgaataa
     3121 aaaagacatc aataaatgta agaaacgacc atacagacaa accagtcagc ataaatagtg
     3181 cgacttttga tacagtggct aaaaccattt ttctatttgt ctgctttcca taaaaactag
     3241 cccgcagact tgctcctacc acaccaccaa aaccggcgag attgttgatc gtgtttgtta
     3301 cccacgcagc aagaagaaac tcttttcttt ccatttttgg ttttccttgt ttttctaaga
     3361 ctctgatcgt cacccaatca tacaacaaca tcggagtgac acccagcagt ccagccagaa
     3421 tcatgagcag gatcgtcatg cggctttgtt gtcccatcct ttgccaaata tcttgccaac
     3481 tcatgccatt agcaatattt gcgacttgat ttgccacaaa gactaacacg gagccgaaaa
     3541 aaatcagttt taaaatcaga ctatgttcct ttatccattg cagcatcaag tatcgtttat
     3601 ttttcacatt ttcccattcc taacttgtat acttttgtgt cataccccaa tgttatctta
     3661 tttattttaa tttttccact ttagacctgt tctttgcttg cgatgataga aaaagaaaga
     3721 tatcctaatg ctaaaggaga ggataacatg aaagtaggat ttatcggcac agggaatatg
     3781 gcacaagcta ttattgatgg tttattaaaa aaaggaacag tctcgaaaga agaggtgttc
     3841 gtttccagtg gacattttga aaatgcacaa aaatttgctg ctgaaactgg cgtccatgca
     3901 tgcaaaagta atcaggaagt tgcatcacaa tcagatatcg ttcttttagc cgtgaaacca
     3961 aaaatcattg aaaaagtatt ggaagaacta agagaatata gccagaaagt cttattcatt
     4021 tctattgctt caggaaaatc attaacagat ttagaaacag cgcttgaaga tcctcaggcc
     4081 tccatcgtac gtgtgatgcc gaatgtcaat gtcagcgtag gagcaggcgt ttctgcgatt
     4141 tgcaaaaatg aagctgccac ggaggaacag cttcagcaag ctacgatgat ttttgaagca
     4201 atcggcagcg tctatccttt agcagaatca gatttcagta cattcattgg ccttgctggc
     4261 agctcacccg cattcgttta catgctgatc gatgcgatgg gacggactgg tgtactgaat
     4321 ggtttgtcca aacagacagc cactgagatt gctgcaaaag caattatggg ttcttgtcaa
     4381 aaattccttt caagtgataa aaatccatgg gaacttgtag accaagtttc ttctcctggc
     4441 ggtacaactg ttgcaggtgt agttgcctta gaagaagcag gattcatccc agcaatcatc
     4501 aaaggaatca atacgacgat cgagaaagat cttgagatgt tgaataaaaa ataatgatag
     4561 caatgttttt tcgtgcctta caaaaaaaga agagctgtgg cttttgccgc ggctcttctt
     4621 ttgtattttt tattatttag cataatcagt tgcacgagtt tcccgaataa cgattacttt
     4681 gatgtgtcca ggataatcca gttcttcttc gattcgttta cgaatatcac gtactaaacg
     4741 aacagcgtct aaatccgaga tttcttctgg cttgaccatt acccgaactt cgcgtccagc
     4801 ttgaactgcg taacttgatt ctacaccggc gaagctgttc gaaatatttt ccagacttct
     4861 taaacgacgg atgtagtttt ctaatgattc acttcgcgca ccaggtcttg ctgctgagag
     4921 cgcatctgct gcagctacaa ggacagagat aacagaagta gcttccacat ctccatgatg
     4981 agaagcgatc gcgttgatta ctactgggtt ttctttgtac ttggcagcta attccgcacc
     5041 gatctcaacg tgagatcctt caatctcatg gtcaagtgct ttcccaatat catggagcaa
     5101 cccggcacgt tttgctaatt ggatatcttc gccaagctct gctgctaaga taccagctaa
     5161 ttttgcaacc tcgaccgaat gcttcaagac gttttgtcca taactagttc tgaatcgcag
     5221 acgtccgatg atcttgatta gatctggatg aagtgtatga gcaccgactt caaacgctgc
     5281 ctcttccccg tattcgcgaa tacgttcatc catttcttta cgagatttct cgaccatttc
     5341 ttcgatacgc gctggatgga ttcgtccatc ttgtatcagt ttttccaagg tcatccgtgc
     5401 gatttctctt cggattggat cgaatccgga aagaacaact gcttccggtg tatcatcgat
     5461 gattaaatcg ataccagtca gcgtctcaag cgttcgaata ttacgacctt ctcgtccaat
     5521 gatacgacct ttcatttcat cattcggaag ggtaacgact gaaacagtcg tttctgaaac
     5581 ttgatctgct gcgcagcgtt gaatcgctaa tgataataag tttttcgctt tgcgatctgc
     5641 ttcttctttg gcacgctgtt cagattcttt taccattaga gttaactcat gattcagctc
     5701 ttcttcagtc gatttcatga tgacatctcg tgcgtcctct tttgataaac cagcaatttt
     5761 ttctagttct ttttgttgat cttcaatcat tttttcaacg tctttttctc gctcttcaat
     5821 taattgctgt tttgaactaa gtgcttcttc tttactttca agtgagttct cgcgtttttc
     5881 taacgagtca tctttacgat tcagcgtttg ttcgcgttga atcaaacgat tctcttgaga
     5941 ttttaattct aacttacttt ctctcagctc actttcaact tcagaacggt atttctgatt
     6001 ttcttcctta gcttctaaca atgcttcttt ttttagagtt tctgcttctt tttttgcact
     6061 atcgatgatt ccagcagctg atgattgtgc accagctatt tctttctcgt gacgtgactt
     6121 tgcgaccata aaccctaaac caagaccgac aattaaaccg atgatagcga ggagaatgtt
     6181 gaataccata aaatccacct ccatactatc tttttcattt ttatcatcta tacagcttct
     6241 atgataaatt attctaaact cgtcaatatg catcttcgca tatatgtgta caaaaaacat
     6301 gcacaacttt attctaatgt ttgtaccccg tagtgtcaac tttaaataaa caaagaaacg
     6361 gctttctact tattcattaa acgatggttt ttaaaataaa tg
//
LOCUS       DAYYHX010000155         6372 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000155 DAYYHX010000000
VERSION     DAYYHX010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.156"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..472)
                     /locus_tag="ACHA6Y_002235"
     CDS             complement(59..472)
                     /locus_tag="ACHA6Y_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8988310.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            complement(548..844)
                     /locus_tag="ACHA6Y_002236"
     CDS             complement(548..844)
                     /locus_tag="ACHA6Y_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988311.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFRGYVEKVLSNSAIIRIENTMECDKWLAKSKENLAVARLVDMEVIESK"
     gene            complement(844..1050)
                     /locus_tag="ACHA6Y_002237"
     CDS             complement(844..1050)
                     /locus_tag="ACHA6Y_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988312.1"
                     /translation="MKIYVVKFGNQFYRSDERSIGANTLSIVDILQSARWFDNLEEAN
                     QVSRRLGGLTQVYELVTVDHEGVE"
     gene            complement(1047..1244)
                     /locus_tag="ACHA6Y_002238"
     CDS             complement(1047..1244)
                     /locus_tag="ACHA6Y_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8988313.1"
                     /translation="MNNRHRRVAKLRNQELNVAKVKFEREYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILDIKWEDTE"
     gene            complement(1268..1573)
                     /locus_tag="ACHA6Y_002239"
     CDS             complement(1268..1573)
                     /locus_tag="ACHA6Y_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988314.1"
                     /translation="MNVQVYLKRDLARFPRSWDTNSHPSEAEEEGYLMSANMIEITRD
                     DADEFDKKRSWYSVPNPMYTAVIEDYHISDRFILIDLERPKKRIKQYRRCGFSTKKW"
     gene            complement(1570..1743)
                     /locus_tag="ACHA6Y_002240"
     CDS             complement(1570..1743)
                     /locus_tag="ACHA6Y_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988315.1"
                     /translation="MSRQIMLHIPDGTKAMTITIVAAQKDGGLALKTKSIDTKQILEG
                     RDVEIEIDEEEAE"
     gene            complement(1740..2309)
                     /locus_tag="ACHA6Y_002241"
     CDS             complement(1740..2309)
                     /locus_tag="ACHA6Y_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM8988316.1"
                     /translation="MNKQEAIKKLESIKVIGNDALAACYNESINAGITLMKKIDEPKK
                     LVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLYSVIIAGEYLVKEISCSNEVKLVSTAYLPQYLAH
                     HYQLTEKQIKAIDERYWPFAVPVEEVAEG"
     gene            complement(2321..2455)
                     /locus_tag="ACHA6Y_002242"
     CDS             complement(2321..2455)
                     /locus_tag="ACHA6Y_002242"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988317.1"
                     /translation="MKDWLEEASALVGCFINCAFISVIIALVGFVSLKVIILLWQLIF
                     "
     gene            complement(2455..2790)
                     /locus_tag="ACHA6Y_002243"
     CDS             complement(2455..2790)
                     /locus_tag="ACHA6Y_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988318.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDIHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEQ"
     gene            complement(2787..3173)
                     /locus_tag="ACHA6Y_002244"
     CDS             complement(2787..3173)
                     /locus_tag="ACHA6Y_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM8988319.1"
                     /translation="MIPRFRSWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRI
                     FSHQKMGYEYEVTGNIHENSELLEVE"
     gene            complement(3170..3487)
                     /locus_tag="ACHA6Y_002245"
     CDS             complement(3170..3487)
                     /locus_tag="ACHA6Y_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFM8988320.1"
                     /translation="MNLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYME
                     GSK"
     gene            complement(3487..3792)
                     /locus_tag="ACHA6Y_002246"
     CDS             complement(3487..3792)
                     /locus_tag="ACHA6Y_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988321.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKTR
                     RRMPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKNMDIDSKGRQFRYVEEK"
     gene            complement(3789..3950)
                     /locus_tag="ACHA6Y_002247"
     CDS             complement(3789..3950)
                     /locus_tag="ACHA6Y_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8988322.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(3965..4324)
                     /locus_tag="ACHA6Y_002248"
     CDS             complement(3965..4324)
                     /locus_tag="ACHA6Y_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM8988323.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(4321..5172)
                     /locus_tag="ACHA6Y_002249"
     CDS             complement(4321..5172)
                     /locus_tag="ACHA6Y_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8988324.1"
                     /translation="MESLASTMEKLIRRVLVQSGRCPECSEPLYSWRGKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(5189..6019)
                     /locus_tag="ACHA6Y_002250"
     CDS             complement(5189..6019)
                     /locus_tag="ACHA6Y_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFM8988325.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLGSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDIDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(6022..>6372)
                     /locus_tag="ACHA6Y_002251"
     CDS             complement(6022..>6372)
                     /locus_tag="ACHA6Y_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8988326.1"
                     /translation="NVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCI
                     CGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGI
                     KLNQETIKKLRIGG"
BASE COUNT         1585 a         1465 c          954 g         2368 t
ORIGIN      
        1 cataatatca ctggtaaata gctaaaaacc gccatcattc cgccaaaaaa ccgccaaatt
       61 atttataagc aattattctt ccgtgtttat atgcttctgc aaactctatt agagcttccg
      121 acttcatccg ttgtatactt ctttctgaat aacccacttc acggctaatt ctgtagtttg
      181 agaagctatc tggcacacaa aagctgtagt agagtatctg acggctaatc agactaagag
      241 ccatcaaagc cgctaaaatc gcatctctct ctgcttctat atccatcatc tgaatgatcg
      301 cgtcctctgc cttattgccg tgcttcggcg ccttcggcat atccgtaata attggagact
      361 taatatctat caaagagcga cctgccatcc gctccaaacg ccgaaagttc ttcagcacat
      421 ctctcgcatt acatcttgtc tgtttgaaat ctacctctcg taacaattgc atcaagtcaa
      481 accgctcctt tatgtgatat aataaacttg tctgttttat tatgttagtc ggagcgatcc
      541 ggctttttta tttgctctcg attacttcca tgtcaactaa tctcgctaca gctaaattct
      601 ctttgctttt cgctaaccac ttgtcacatt ccatcgtgtt ttcaatacga atgattgctg
      661 agttcgacaa taccttttct acatatccac gaaatggata gataaatcct tctgcttcac
      721 agcgaaccat gtcaccgact ttgaattttg gtttctcacg tgttttaggg ttctttgtcg
      781 gcatatctag catcaaaccg ccgataccat gactactagc gtaaaatccg tcttttagtt
      841 tcattactct accccctcat gatcgacagt gaccagttca tatacttgcg ttaatccacc
      901 aagtcgtcgt gaaacttggt tagcttcttc gagattatca aaccatcttg cgctttggag
      961 tatgtctaca atggataatg tgttagcccc tatagaacgt tcatcgcttc tgtaaaattg
     1021 atttccaaac tttactacat aaatcttcat tctgtatcct cccatttaat atctagtatc
     1081 gaaattccaa acttacgaat agcatcactt gcatcagcaa cacactgact tgccacttta
     1141 tatgtttctt ctgcagaaac accatactcc cgttcgaact ttactttcgc tacattcagt
     1201 tcttgatttc ttagttttgc tactctgcgg tgtctgttgt tcatttttct tcacctctgt
     1261 tttgttatca ccatttcttt gttgaaaatc cacaacgtct atactgttta attcgtttct
     1321 taggcctttc tagatcaatc agaataaatc tgtcggatat atgataatct tctattacag
     1381 ccgtatacat tggattcgga accgagtacc atgatctttt tttatcaaac tcatccgcat
     1441 catctcttgt aatttctatc atgttagcag acattagata gccctcttct tctgcctcgg
     1501 atgggtggga atttgtatcc cagcttcgag gaaacctggc caaatctctt tttaaataaa
     1561 cttgaacatt cattccgctt cctcctcatc gatctctatc tcgacatctc tgccttctaa
     1621 aatctgtttg gtatcaatgc tttttgtttt caacgccaac ccaccgtctt tttgtgcagc
     1681 aacaatggta atcgtcattg cctttgttcc gtctggtata tggagcatta tctgtctact
     1741 catccttccg ccacctcttc cactggcaca gcaaatggcc agtatctttc atcaattgct
     1801 ttgatctgtt tttcggtcag ttggtagtgg tgtgcaaggt attgtggcag gtaagctgtt
     1861 gatactagtt taacttcatt actgcaactg atctccttga caaggtactc gccagcaatt
     1921 attacactgt acaacggctc tttctcgacc tcgtagccgt tgtacaagct caatagtgtt
     1981 tcatatgatt gtctcgttgc ccaagcgagt aattcttctc cttgttcttg cgtcactatg
     2041 ccatctcgta caaaccaatc acagaaataa tatgaatcac catctttaga tttgattaaa
     2101 tacgctattt tttcagcttt cgtaaaaggg tccgtagatt tctcaagcca accggccacg
     2161 agttgtggaa taactagctt cttcggttcg tctatttttt tcattaacgt aatacctgca
     2221 tttatactct cgttatagca agcagctagt gcatcatttc ctatcacttt aatactttct
     2281 aactttttga ttgcttcctg tttattcatc gctgttcctc ctaaaaaatt aactgccaaa
     2341 gcaatataat cactttcaat gatacaaatc ccaccagtgc aataattaca gatataaacg
     2401 cacagtttat gaaacagcca actaaggcgc ttgcttcttc taaccagtct ttcattactg
     2461 ttcctccttc aaccataaat ttggaaaatc atcggcatat atctctattg cgccaaataa
     2521 cttgatcgct gattttgatt ttccatgtat gtcaggactg attaaacgat cataataatc
     2581 ctgactgatt gtttttccct tgtactgata acgtgtagcc gcagtgtaat atactgctgt
     2641 cttgctcaga taaagatatt ttgctaaatc agccgatatc cgtaacaaaa tgattctatt
     2701 cttactttta attccacaat ttagcaatgc tgggacattc ccattttcat cttttaaaga
     2761 atcgatgtag tcagtagcta agataatcac tctacctcca ataactcgct attctcgtgt
     2821 atatttccgg tgacttcata ttcgtatccc atcttttggt ggctgaatat tctatattcc
     2881 attccactaa ttaatgattt acaaactaat ccggaatgaa actcggattc ttgtacaacc
     2941 gttttttcat taactaaatg atcaaaacca ttacttacat taaccagaac tacatcccct
     3001 tcaaatattt ctacgccatt cttatctttc agtcctgtgg attgcataag cacatattta
     3061 tctggagcca tttcagcatg agtgatcaac cttcctgctt gcccatattt catttcttgt
     3121 ccaattgttt tacctttaaa tggtgtgtac cacgatcgaa atctcggtat catttgctgc
     3181 cctccatgta ttcgtctaat atctctttat atttctctac aaatttgaaa cgatcttgat
     3241 gaagtttctt gctccaattt gtttgccgat ccagctcacg catttgatcg aacccttttt
     3301 gaatttcgtt gtaataaaat tcaatgtttg ctgctgcctt ccaatgcctg ctacttcgca
     3361 ctcctgcccc tgtttcagcc atttccaact taactaattc cgctcgttct tttgattttt
     3421 tgtctttctg aatcttcatc atgattttct tgaggatgat atcactgtat tgtgtaatga
     3481 gattcattat ttctcctcca catacctaaa ctgtcttcct ttagaatcaa tatccatatt
     3541 cttcgctcta tcccagatga tgtttttgct cagaccagta atttcagata actgttcagc
     3601 ggtacctgtt actagaattc ggtcaccgtg ccagattgca atttttctcg gcattctccg
     3661 tctggttttt tcagtccaca ttgatttacc gagcttttgg acttctgcaa ctatttcttt
     3721 gtcttcttgc caattctcag aatgtgtcag ttcgatgatt cgtttcattg ctgctttctt
     3781 atccacgctc attcctccaa tctacgaatt tcccttctta agttctctat gtgcaaatcg
     3841 attgcctttc tcgccgtttc attgaccatc actgcctttg ttcgttccag atcgtcaatc
     3901 tcacgttgaa tgcttcgaat tcgcatttga atcacttctt ctgttgtcat gatagaccac
     3961 ctctttaaaa acgctcttcc ttgaacgtat tccgatattt tttagctaat atcaacggca
     4021 cttgatattg atgacaaaac agctttgcct tgatcttaaa gtcttttgtc tgcattcctt
     4081 taacatctac gactttgaca agtttgccgt ttttataaaa tgtgaagtcg ggaatatact
     4141 cgatcttgcg atacttcttt ccgtctagtt caaatttcgg catcagctca aatctttcct
     4201 gaagttttac tttccagcca ttcgcttcag cttgccacaa ggctagatcg taatactctg
     4261 cttctgcgat agaatcaaac ttgatacctc gatgaacagt ttttttatta cggtatttat
     4321 tcatgcgata ctacctttca ctggttttat gcgcttgtct gctgtttgct ggaatttcag
     4381 cgcataacct tctgaattct taaatatcct agaaacaatt ctttcaccgt aggcttctct
     4441 tagttcagga ccagataagt ttgttgtgat gatcgttgcc ttgttctgtc ttgcttctaa
     4501 gagcgtgttt aacgtgttgt ttgtaaactg cctactattt gataccccac tacctaattc
     4561 agctccaata tcgtcaaaaa ccaccaaatc agttgtttta atatcggcta taagcgatcc
     4621 ttcaatttct tttctcagtt cagcattgtt ataagaaaac tttatttgct ctaataattc
     4681 ttgatagctt ataaaaagta ttttcttgtc ataatttgag cgctcaagta tttcccaagc
     4741 tgtagccatt gataagtggc ttttcccgct tcctgatttc cctgatagaa tgaaatgtgc
     4801 aggatggttc agcagaacct cattcgtata gctttttgct ttttctaaag cgatttttgt
     4861 ttcttgatcc actacgtgat aattttccat tttgcattta aacaaagttt tatctgttaa
     4921 taccgaacca ttttgaaaaa aactcaacgc tcgtgctttt aagctgtcgt tatatatccg
     4981 ttcggtctgt atatcctctt tcacacgtaa cgctttataa ccacaactca tgcatgttgg
     5041 tttacaacgt tctgacccgt ccttattttt ccctcgccaa ctatacaaag gttcgctgca
     5101 ctctgggcat cttccacttt gtactaaaac tcttcttatt agcttttcca tagtacttgc
     5161 taggctttcc atgtgatgca tctccttttt aaattggcaa gtcgtcatat tcactaggat
     5221 tgctgtactg taatttttga ctttgctttt tatgattatt tttatctgcc ttgatttcga
     5281 atttaagctt ctcaaatttt tctcgcagtt tcttagcact tctaatattt ccaaaccaaa
     5341 attcatttgt aggtagccaa ttgatcacat actcaatcgc ttctatagat gctttatctc
     5401 tttcttccat caacctgatt gtgtctgccc atttttcgat atctacttta ttcatttctt
     5461 ttggaaaatc ttcagttaaa ttactttgca actttttagc aaggcgtaag tgttcgtcag
     5521 aatacttacc tttcttttct tctttatcta tatctatatc ttcttctata tctttatctg
     5581 taccgtcacg tgacgtcacg ctaacgtcat tttccaattt gagacgttcc tgtctctttc
     5641 tttctctgta tttacggttt ctttcagcgt tttttagcct tactttatcc atcccttcga
     5701 tattttgatg tttttcccaa ttactgatgg caatcagtcc atcactgcct agatcaatca
     5761 tgttgaaatt tgccaatgta gttagcgcta ggcgaaccgt atttacgttt ttgccgaaca
     5821 atgtagcaag catttcttcg gtataaggca tgttcctctg gatatatatc agaccatcgt
     5881 cgttagtctt tcctgctaaa actagtaatc gaatccatat aacgatgatg gcatccgact
     5941 caggaacagc ttggattaat cgtatttttt catcgtcaaa catagtagtt ttaagtttga
     6001 tccaacttat ctcagccaaa tttatcctcc tatccttaac tttttaattg tttcctggtt
     6061 taacttgatt cctttgattt gatatttatt tttgaaattg atcacaccta ttttgtgttt
     6121 ctctgtgtga tggattctgc agagtgctgc aaatgtgtac tctgaatgat caacttcctt
     6181 gcgctttcgt cttcccagcg ctttgtcaaa gtgatcgata tcagctcctg ttttgccaca
     6241 gatgcagcag actctttttg taatgcattt gtagaagtaa tactcttgat tcgctggtaa
     6301 aatctcatat ccttctttga aaggaatatg atgttcaaag atgaaatcta aaatgatatt
     6361 tgctaagaca tt
//
LOCUS       DAYYHX010000156         6341 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000156 DAYYHX010000000
VERSION     DAYYHX010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.157"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHA6Y_002252"
     CDS             complement(99..1229)
                     /locus_tag="ACHA6Y_002252"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFM8988327.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHA6Y_002253"
     CDS             complement(1878..3080)
                     /locus_tag="ACHA6Y_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8988328.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHA6Y_002254"
     CDS             complement(3140..3343)
                     /locus_tag="ACHA6Y_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8988329.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACHA6Y_002255"
     CDS             complement(3485..4552)
                     /locus_tag="ACHA6Y_002255"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8988330.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHA6Y_002256"
     CDS             complement(4610..4717)
                     /locus_tag="ACHA6Y_002256"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8988331.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHA6Y_002257"
     CDS             4880..5692
                     /locus_tag="ACHA6Y_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8988332.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6266)
                     /locus_tag="ACHA6Y_002258"
     CDS             complement(6066..6266)
                     /locus_tag="ACHA6Y_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988333.1"
                     /translation="MYIFEQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKE
                     LEFENALVICMTCTDEVFRKHF"
BASE COUNT         1893 a         1300 c         1000 g         2148 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata a
//
LOCUS       DAYYHX010000157         6180 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000157 DAYYHX010000000
VERSION     DAYYHX010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.158"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACHA6Y_002259"
     CDS             complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACHA6Y_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM8988334.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="ACHA6Y_002260"
     CDS             499..861
                     /locus_tag="ACHA6Y_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8988335.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="ACHA6Y_002261"
     CDS             913..2595
                     /locus_tag="ACHA6Y_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM8988336.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDDIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="ACHA6Y_002262"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="ACHA6Y_002262"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM8988337.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="ACHA6Y_002263"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="ACHA6Y_002263"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM8988338.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="ACHA6Y_002264"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="ACHA6Y_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8988339.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2056 a         1078 c         1417 g         1629 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tgatatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaagaatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
//
LOCUS       DAYYHX010000158         6084 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000158 DAYYHX010000000
VERSION     DAYYHX010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.159"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..541
                     /locus_tag="ACHA6Y_002265"
     CDS             185..541
                     /locus_tag="ACHA6Y_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8988340.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            551..1450
                     /locus_tag="ACHA6Y_002266"
     CDS             551..1450
                     /locus_tag="ACHA6Y_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8988341.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            1970..2698
                     /locus_tag="ACHA6Y_002267"
     CDS             1970..2698
                     /locus_tag="ACHA6Y_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8988342.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            2843..4870
                     /locus_tag="ACHA6Y_002268"
     CDS             2843..4870
                     /locus_tag="ACHA6Y_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM8988343.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            4978..6075
                     /locus_tag="ACHA6Y_002269"
     CDS             4978..6075
                     /locus_tag="ACHA6Y_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8988344.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         2107 a         1006 c         1258 g         1713 t
ORIGIN      
        1 gctttttggg tctttttccg ttcaagaaac tgaacgaaga aaagggaaag ttgtctcttt
       61 tgtgataaga gaaatcataa taaacgaatg acttactttc caataaattg agaacagtca
      121 gaaccagaca aattagttgc atctgatgaa taaagtgatt attctatgac atgaggtgat
      181 acagatgaaa ttagaagtga caaaagaagc acaagaagta ttgaagaata aattagaagc
      241 agacgatcaa cttttattag atatcgaaaa cggtgacggt ccatttgcag aaagccaagt
      301 cagttgccaa ttggatacgg catttcgatt gatcattgtc aaaaaagata cacaaacaga
      361 tctaagtctt tattctgtag tggctgatac accagttgga ccaatcaaaa tcaaagacag
      421 tgcaatactt tacatggatg atccatccac acttgcttta gaaccaactt ataatagttt
      481 acaattaaaa ggaccaagcg gcttgagaaa aggcaacttg caagttgttc gaaaagacta
      541 gagggataaa atgggaaaat tggcgatcat cagcgaccta catgtggaca tcaataaatt
      601 cggcgaatca gagctgatgc agctttggca agttttgcaa aagcacaaga tcactcggct
      661 tcatctagcc ggagatacgg ctaataaagt agaccgttgc ctagctgttg tcgaattttt
      721 cgtcaataaa ggattatcta cgacatttca tttcggcaat cacgagttgg cagatgtgcc
      781 caatgaacag atgatggacc attttccaga cccacatttt ttaaatgaac ggtatattgc
      841 tttaaatgag cagaccgttc ttttagggat gaatggctgg tacgactatc agttttccga
      901 attgaaagat tacgaggcaa tcgtacgaat gaagcgtttg tattggtatg atcggttcat
      961 ccagcgtggg aaaacagatc tcgaggtcaa tgcagaaatg cttgcagctg caaaaaattt
     1021 gctagaccag ctggaagcaa aaaaacttga ggtgatttta tccactcatt tcgttcctaa
     1081 acgggcattt atcatgtatc aatctgctcc ttatgagcgt tggaacaaat taaatgcatt
     1141 tttaggatca gaatcattcg gagaattact tgaccgttat tcaaatatca aacaagtagt
     1201 ttttggccat acgcatcgtc gctttgaaga taaatccatc ggtcacacga tttacagctg
     1261 cagacctttt ggttattatt atgaatggca gctgacccga gattttgtct tgaaagaaca
     1321 gttgctagac agctatgatc ctatgaaatt acgtgtcctc ttgcgtgccc atcacccagc
     1381 ttttcaggcc tatcaaacac aacatttgca agaggaattt gaacaagcga tgacaatcgt
     1441 tccgtattga gacagcatta gtaatgtgta aaagaaaata ccttgtaaaa cataaggata
     1501 agtaagagca gtagatccat ttatcgtgga tatgctgctt tttttgcgga aaataatcag
     1561 cgaatgttta tttgatcgga tgatttgtaa ctcgcagagt actagatgca agcgtgtatt
     1621 ctttttttga acgataatca caaaaaaaaa tataatatac attttttatt taattataat
     1681 taatattgat tgaagtctag gcagtcaagt cacattttga tagataggag ggggacttac
     1741 gaattatttt aaaggctatg acagctgtat ttctagattg tgtttgcatc aagttgcaat
     1801 aaaaactaga aatcaaatcg ggctgataat catttataac taatcgatac ataggaccaa
     1861 atataggtct atgttctttt atttcataag tatcgtaaaa cagaattttg ctatggatta
     1921 atccaatcga ctgatatgtt tagtaaataa agaaagagga atgtcaatga tgaaaaaaga
     1981 aatcgtatta acagcagcaa tgatgttatt cagcatggta gcaagtacga cttttgtttc
     2041 tgcaactgaa gtatacccaa aggaatataa cacagaagga acgattacat ttgaagcagg
     2101 agatgagggt gtaactcctc ctgttgatcc agagaatcct gatcccaata aacctgtcga
     2161 tccatcagat cctccaagcc ctggaacagg aggggcatta tcgattgatt atggatcaaa
     2221 attcaaattt gggactcaaa aaatttctac tgcggataaa acgtattatg cagctgctga
     2281 tgtgatgaac gatgggtcta gaaaaccgac atatgtccaa gtgacagata ggagaagtac
     2341 cttatctggg tggaaattaa gtgtctctca accagaacag tttaaaactg ccagtgggga
     2401 tgaactagtt ggagcacagc tgaaatttac gaaagggcag gctgtttctt tggttgatcc
     2461 aacatatacg ccgcaaacgg tgaattctga attaacgttg acaccgggag gaaacaatac
     2521 gttagcaatt aatgctaaat caggaacagg tgtggggact tgggtctatc gatttggtgc
     2581 aaatgaaaat gaaaatcaag atgcggtaca gctttcggta cccggtaaat ctgtcaagtt
     2641 agcgcaacaa tattccacta aattagtttg gacattagaa gatacaccta acaactagtt
     2701 tactgaaaag aactgttaaa aataaatacg tagtcatatt gtcttgcgag gtaatcgagc
     2761 aaaaattgtc aaaggctagg ctaaatttgt ttggatacga atagttcatc taggaattta
     2821 acgatcagga ggaaaagata tattgaaaaa gaggaggatc gtgctcattt taagcagtct
     2881 cttagtgact aatatctttc cgatcatagg gtactctttg gaattgaatc agttggagca
     2941 tggaaataga agagacgaag aaagcaaact cacttcgaca ttcgacagtt taatagaatc
     3001 caaccagaca caagaaagca gtgaggattt aagtatttcg agtagtggag agaccactga
     3061 caacgtagaa gctgagggtt cggacaaaca aggactagaa gatgcaccaa tttctgcggt
     3121 tgagctgcct gacgatccca atattatttc tatcgataaa atttttcaaa cacctatcgg
     3181 tactagcacg tccatattgg aaaatgggaa gttactgcag ttgaacccag cagaaaggtc
     3241 tcaaagagga gctatttggt caaataagaa gattagttta ctctcggatt ttacatttaa
     3301 atcttattta tacttaggaa atcaacgaga tagagcggga gatggcatga cctttacatt
     3361 gactggcgat cctagaatgg ctagtaatcc cagtcaagtt attggatcgc caggtatggg
     3421 aattggctca tattctactc gttctggcca accttacatt gcgaatgcgc tctcgattga
     3481 gtttgatacg tataaaaata gtggttcttc tgatcgaatg gatcgagaga taactgctga
     3541 taatagaggg catggacatg tcgcttttgt cacgcctaaa ccaaataata ataattattc
     3601 tggagagcat actggcgtga cggtagctcc tacatttctt tctaatggca catggcgctt
     3661 gttaataatt aaatgggacg cggagcaaag aagtcttact tatgatttgg aaggtgtcgg
     3721 tcgctcttct cggattatac ctgatctcat taggcagttt ggttctacag aagtaaattg
     3781 ggggtttact tcttcaacag gtactttttt tcaagaaaat gctgtcgctg tcacgcgact
     3841 tccatcgaat gtcacatcaa cagctgatgt aaaagtcaac gatagtgatt atggggccat
     3901 cgctgaagct ggaggaaacg ataagataac tatcagaaat acattgaagg tcgaggggga
     3961 ttttcttgaa tataagaaaa tgcagactag tatcgacctc ccacaagaat taactattcc
     4021 cgagcatatc ttgatcaatg gaaatcaaat tgatgccaaa gatatcttga taaccggaaa
     4081 tcgagtcacg ctaaatctga cagattattt tattgctgga gatacgttaa taatcgaatt
     4141 ggagacaact ttgcaggaag taattcctga aaaagtactc actatgcatt ttgaatacct
     4201 tgaaaacggg aatttgattg agcaatcgaa tgaaataaac ctaaagatcg caaagctgaa
     4261 ggagatagat atccatgttt attataaaga taaagacagt cagattgata ttgctgaaat
     4321 gaaaacaatc actgggaaaa taggagagaa gtataaagaa actcctattg atattgaagg
     4381 gtatgttttt gactcggatt cagcgaatac agaaggcatc ttctccgaaa atacagagga
     4441 tatctatttt tattacagaa aaggcaaact ttatctctca actgctcctc aatatgtcaa
     4501 ttttggaaaa cataaaatat ccagtagacc attaagtgaa ttcggacagt tttcaggtgg
     4561 gttgaaaatt atagatgaac gggcttttgg aagttggaaa ttacaattga aacaagagaa
     4621 agttcttaca aatggagatt ttgagcttcc agaagctctt tcctttgtta cctctgaaaa
     4681 tacaacggtt atagggacat ccgctgttac gattttcgaa agtgatcaaa agggtgaaag
     4741 cgatttgtcc tctcttttag attctacagc tcatcgtgga atcagaataa atattcctgt
     4801 agaaaaccaa agaatcggaa cctttgaggg gaaactatct tggatacttg cagatgtccc
     4861 aggaaattaa caggaagaga agaatataac aaaattattg agtttcaaga agggcacagg
     4921 taccataggc tcttgtgtcc cacagagaca agattttgtt atagtcttct cttacatatg
     4981 attaaaggag cgattttctt gagaccaaaa caacaattat ttataacact cctagttatt
     5041 ttaggggcac tttttaacaa tatatcagtg tcagctagtg catttaattt ttcagtgacg
     5101 ccgataactt cagagaatca aatcgacaag agaaaaactt acttcgatct tcaacttgat
     5161 ccggatcagg aagtagaagt aaaagcggaa ttgagaaatg atacagaaaa agaagtgaaa
     5221 atcgatattt ctgtgaatag cgcaaccacc aattcaaatg tgatggttga atacggaaaa
     5281 aacgagattg aaaaagacat gagcctgatc tttgatctgg tagattatgt gagctatcca
     5341 cagatagtga cgctaaaacc taaaagtgtc caaaccgttg cttttcatgt caggatgccg
     5401 aatgagcgat ttgatggtgt attggctggt gggatcacat tccaagaaat ccagacggaa
     5461 aaggaccaaa cagaaactaa ggatcaaagc ttatcgattg agaatgaata cacctatatt
     5521 attgctttgt tgatgcaaca gacactgaac gaagttgctc cgaaccttac tcttcatgaa
     5581 gttaaaccag atcaaatcaa tgcaagaaat gtcatcttag caaacgttca aaatgatcaa
     5641 aaaacctata tcaatcaagt agttattgaa acaaaaatta caaaaaaagg acattctgaa
     5701 gttttatatc aagaggaaaa agaaggattg cagattgcgc cgaatacaaa tttttctttt
     5761 ccaactgctt taaatggtca accgctaacc ccaggtgaat atcaccttac aatgactgtt
     5821 ttaggaaatg aaaatgaaaa tggaaaattt tcgagaaaaa aaggaaatac tacgatcaac
     5881 tataccaatc aatgggtatt tgaaaaagat tttacaatag acggaaaagt agccaaggaa
     5941 ttgaacacga cggatgttac gattaaaaaa gacaataatt ggatttatat cttagtagct
     6001 tcacttttct tattgtttat tttattatgc atacttttgt ttgttttgag aaaaaaagaa
     6061 gagaaagaaa agtaaggtta agat
//
LOCUS       DAYYHX010000159         6059 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000159 DAYYHX010000000
VERSION     DAYYHX010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.160"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(202..489)
                     /locus_tag="ACHA6Y_002270"
     CDS             complement(202..489)
                     /locus_tag="ACHA6Y_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8988345.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(509..733)
                     /locus_tag="ACHA6Y_002271"
     CDS             complement(509..733)
                     /locus_tag="ACHA6Y_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8988346.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1188..1358)
                     /locus_tag="ACHA6Y_002272"
     CDS             complement(1188..1358)
                     /locus_tag="ACHA6Y_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988347.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1373..1906)
                     /locus_tag="ACHA6Y_002273"
     CDS             complement(1373..1906)
                     /locus_tag="ACHA6Y_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8988348.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1927..2871)
                     /locus_tag="ACHA6Y_002274"
     CDS             complement(1927..2871)
                     /locus_tag="ACHA6Y_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8988349.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3508..3594
                     /locus_tag="ACHA6Y_002275"
     CDS             3508..3594
                     /locus_tag="ACHA6Y_002275"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8988350.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3715..4404)
                     /locus_tag="ACHA6Y_002276"
     CDS             complement(3715..4404)
                     /locus_tag="ACHA6Y_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988351.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4411..4689)
                     /locus_tag="ACHA6Y_002277"
     CDS             complement(4411..4689)
                     /locus_tag="ACHA6Y_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988352.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4649..5563)
                     /locus_tag="ACHA6Y_002278"
     CDS             complement(4649..5563)
                     /locus_tag="ACHA6Y_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8988353.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5545..5925)
                     /locus_tag="ACHA6Y_002279"
     CDS             complement(5545..5925)
                     /locus_tag="ACHA6Y_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8988354.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1603 a         1112 c         1022 g         2322 t
ORIGIN      
        1 aaagtttcgc ttgtttcacc ttcggtgaaa ttgttgcaaa tcaataatta ggtagagcta
       61 catttttttc gttgttcaaa gattcttatg ccatttacta ttaataaatc tataaatatg
      121 ataatttttc ggctctataa tttatgggac tgatgaatca gaattgattc ttcagtccca
      181 ttttcatttt attccaaaga attaactcca aataccaata gaagcccatc ctctatatac
      241 ttgagattcc ttactaacaa tttcttgtaa ttctattaaa gatttaggta atccaaattt
      301 atgttttaaa atatatcttc caagaaaact acttaatttc atactaactt caccaacact
      361 ttttccctca ctgatttctt tagaataaag aaagagcttt tcttttaagt caggatagtc
      421 atctatagtt gcttcattgt atactttact aattgcattt aaaagttttt cttctttagt
      481 aaaatccatt ataataacct ccctaccact accttctagg tgcccatgga ccatgattaa
      541 cccaaccatt aacaataatt tttccaattt ctcctttagc ttgattccaa tctacccaac
      601 atttttcttt gttacaataa agaccatttc catagtaagt agctgcatca acttttattc
      661 ctgtaccgat gccagctaga caagttccta atattcctag aataacacaa tgttttaata
      721 ctttcttttt catgattgtt tttccttttc gtttttttaa aattgcttgt tatattaaat
      781 aatcttagta attttattat ataacatttt attatataac aatgattaac gtcataaaaa
      841 gtagttttga attcataatc gtttaaataa aattataaga tcagttattt aaacagctag
      901 tagacataat gtatatacta ccaagtaacg cctgtaccaa gggatccagg cgctgatttt
      961 gtgaattttt attggtctag gttaaaagct ctttttaagg cttggttttc tgcggttgtt
     1021 tctttctaat tctttgtgaa tattgatcgg tcatttttta aatacttgtt atttttgttt
     1081 gtgtttgtta tgctttttac atatctcata ttcatttatt attcttttct aaggttaata
     1141 ataggataat ctctttcaga aagactgctc gtacgagcag tcttttctta tttatcaagt
     1201 ttttctacaa atacttcctc attttgactg ttgtagtaat taggattgag ttttagttcc
     1261 ccttttttat ttcctttgtt ggccactgat aataacattg ggctaaagct taatattcct
     1321 agtaggatag aaccgaatag caagtgtttt tttgtcataa gtatttcctt cttcatttcc
     1381 atagctgcca ccattttttc caattctttg tttgttcgtc cattgtttct gttgcttcag
     1441 aaagagtcgt tgaactattc tctgaagact cttccgtata agtcaactgc agttgctcct
     1501 gaagtttttc gatttgttgg ttggcttgca gggtgagttg ttgttgctgg tcgagtagtt
     1561 tttgtatatg agaattattt tgctctttga gggcaagttg ttctcgtaaa aattcatttt
     1621 cttcacgcaa ctgatctagc aacttgtttg caaccacgtt tttgtttggt atataagcat
     1681 ttttatcgtt gttttgttca tgtttatgca acttatctag caacctttga ttgtctaagt
     1741 tgcgagcaag ttgcaatact ccagaacgca atacaacctt ataaccacgc tcatttttag
     1801 ctatcatgga agaatctaat ttttggatat gtttcgatat tgcttgccta ctcatcttaa
     1861 aaagatctga caattccttt attgtaaagg tttcttgtgg ttgcatactg gttgcctcct
     1921 aggttgctac tggaataaat tatctaaatc agataataaa ctttttttct ctttttccga
     1981 aagtgcggac tctgtttgtt tttcaagagc ttttttctct ttttctagtt gtttgatttg
     2041 ttggaggagt tcaagttctc gtttcttctg tttgattttt tcgttaattg ttgtagcggg
     2101 ttcttcgtat tctttttgtt tatcagctaa agccaatgct ttattttggt cttctttggt
     2161 tggtgcatgt ttgttaaagt cgtctgcttt tttgatctca ggtttgaacg taaattgata
     2221 gccaataaca ggttttcccc gtccttttcc gtatttcttt ttgattgtta gtcctttgaa
     2281 gattgcagtt agttcttgac gtataggttt tataactcgt ttatctatat caggttgctt
     2341 ataactttta ggaatatcta ataattcaag aaagtcttct ttggagaaat atgcgaaacc
     2401 tttggttctg tattgcttta acagacgaaa catagtcttt gcatagccac tttgtagctc
     2461 gttgaattgt tgtaaagaat agcgtaccca ctcgtctaaa ttatttaaca aaggcagtgc
     2521 tttttcgtaa attcgtatat ctacgtaagg attttctacg tccccgttga ttttaaattc
     2581 tgtaaacatt acaaacatat cgtaagaaag cccagactca cttctacgac caaagcgtaa
     2641 agataagatt ttttcataag ttgcttttaa atcttctaca aatcgtacat ttgcagttgc
     2701 tttgtagtta cttaaatctt ttagttggtc aaacgtaaaa cgaactgtct tatccccttt
     2761 ttcacgcatt ctagacacga tggaaaaaaa caaattcatt tcaaccggtg taaattttct
     2821 taaaggaatt gtatttaatt catggtggta ttttacaatc tcattagcca tataatcacc
     2881 tcaatccaat tatgaatgac attttttaaa tgtcaagaca ttcatatttg acgttcaaac
     2941 agacaaaatg tccttcataa acagacaaaa tgtccttcat aaacagacaa aatgtccttc
     3001 ataaacagac aaaatgtcct tcataacatt ctgtatccat tggtagagta aggacacagc
     3061 ttacggtaaa gaggtttaaa aagaggttta aaaagagata taaaaagagg ggggctaatt
     3121 ttcccgccta aaaaccactt cttttttctt taaacaggtt cgcgcatttt tcaaaactgc
     3181 caatccaaaa ccttgcttcc tacggtcgcc aaaaaggtgt gatcgctacg ctctcacatt
     3241 gtactgcgca gtcgcctaaa ggctcctttg ctaaaaacat gttggtaaag aggcctttga
     3301 gcggttctga atgcttatct aagccgtttt aagacagtct tggataaaaa tccttatcta
     3361 gctttaaagc gccttacagg cggtcagggc aagccctaac aaccctaaaa taagcgatac
     3421 ttaaaaacac cacacaagtt taatttttta agatataata acttgtcgcc tatccgacag
     3481 tcttgcgact ggaaggaggt gtatctaatg tttgaattgt tcatttgccc ccttcttata
     3541 gaagtttgtg tctctttatt cagttattgg ttgaataacc gaaacaagaa gtaaaatcaa
     3601 taaaggcgac atcaacctga aaaaaagccc cagagaatcc aacctctctg gggctttcgt
     3661 gtatctaatg tttgatcatt tgcatgttta gtttaacatg attgccgatc aaattcaatt
     3721 ttgctgttgc tgatctaacc attccttgac actttttaga taatttaatg ccgtttcttt
     3781 tttcgttgga ttttgtatgt tcgttgcttg ctgaagcagt gctttataac gttcgtaggt
     3841 aactgattgt tgccgttctt ttcgttcctg ttcactagtt gaaaaaggga ttgtatcact
     3901 gaataaatta taacaagctt cttctgtctg cgtatcttta aaatattgca cgtctttttt
     3961 ggttcgctca tagttgtctt gaacctcttt ttgctgtttt tctggcaagt tttgataaag
     4021 acgagtgagt ttttccatgt ttttatccgt caaacgtttt tcttgattca caaaaatatc
     4081 gttcatttcc tcataacaag tggtaaactc ttccattttt tgtgttaaca cttgctcctt
     4141 tttcttttta tcacatgctg cttcatactt ttgagtgata tcccctaata aaatacttct
     4201 tgaaatacgt ttatttccct ctaaaacgcc gaataaacca cttaaaacga ttgataatgt
     4261 aaatataacc cctggcaata aaaacctctt aaatcgaatt ttagagcctt ttaatgaata
     4321 tcttgtttga taagccttat agctaagcca aataattcct aaaatactgg caaaaatgag
     4381 tagatagggt aaatagaatg ccatttctat ttattctccc cataccagcc aaaaggctaa
     4441 cagtgtcatg attaagcaac ataagggggc aagaatatcc attgatcgct gcataattac
     4501 ttttttctgt tctttatttc gctggtttaa tagcgcatgg tggaattttt tgacttgtga
     4561 atcaattgta gtttgtattc tttctttggt ataaggtgca agaaaagcac gttgtttctt
     4621 tatttcattt tctatgtctt gtaacgtttt agaacgataa ttcttgttgg tagtctgctc
     4681 ctctttcaac gtgttggtca tggtttcttc gagtcggtct aatttccggt tgttcagtcg
     4741 taagagttcg ttgttctctt cgtacttctt ttgcagtttc gcgaatgcta gctcgagctc
     4801 ttcgtagctc gttggttcgt tgttgattgt aggctcgttc tcgtctgtcc agctcatgta
     4861 aaatcgtctc cttttcgtaa tcctttccta gtttgttgga tcgtactttt ttctttttgt
     4921 ggatcaattc gtaagtaaaa tttttcccac gattcaagca atgaatgccg aatgttttaa
     4981 gagaatcaca aaattggtta tacgttcgag tttttttgtt tttcattgtt tgatcaatcg
     5041 caaaccgaat ttcgtctttc caaacaggtt catttttttc ttttaatttg atttcagaca
     5101 tggttcgttt ttcgggtttc ttccctcgtt cagtgattgt taagtcatgt tcaagacaaa
     5161 tctcgtcatt taggtcataa catttgttta aaaatgcttg accatgggct tgaaatttgt
     5221 tcccagtttc cagattgacg gagttaatga caatgtggtt atgaatatgg tctttgtctg
     5281 tatgtgtgta aatcgcgatt tggtggtcag gggctatttt tttcgcatac tctgccccta
     5341 gttgattggc tttttcagcg gtaatttctc ctggtcgaaa agactggatc aataagtgtg
     5401 cttgtacacg atcgtctttt ccatacagca tgcgaatttg tttcatttcg cttttggcat
     5461 aatcaatgtc acagtttatc cctgttttga cggttgctcg tggttcggcg taattgatcg
     5521 ctcggctaca agaagtggtt cggcttactt taactgttgc catatctttt gtagctcctt
     5581 ttgcatgatt tttaggtttt cggataagcg ttgggcgtcg ccaggatcaa cgtcgtgttg
     5641 gtgtgtgttg caccacttgg caatttgatt gatattcccg ccagctttgg ctaaatcgcg
     5701 cgcgattgcc tgagcaatgt ctggcgccac tttcggtgtc accagacgcg cgttttgtgc
     5761 cttggctttc acgaaagccg gcacactcat ttgcaaagct tcagctgact gcttcagctt
     5821 tgcaaattcg gattcgctca ctcgaaaact gatttgtttt ggctcggatc gataggtata
     5881 ttttttagaa tgattgctag ccaaatattg attttgttcg ctcataagtc ccaccctttc
     5941 agtacgtcct acggacgtat cgttcgcaat accattgtac ctaaaaaggt acaataagta
     6001 aagctcacta aagcttacta aagggcttgg caagctctat atttgctagc caaatacct
//
LOCUS       DAYYHX010000160         5988 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000160 DAYYHX010000000
VERSION     DAYYHX010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.161"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..230
                     /locus_tag="ACHA6Y_002280"
     CDS             <1..230
                     /locus_tag="ACHA6Y_002280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988355.1"
                     /translation="FRVAVFIIAPSLLFFIYFYQLLLSKAHRNFTSPQVYGEPYGSII
                     IPTLNHRFLLTIKIILEYLSLLLYSKKSWRN"
     gene            complement(376..621)
                     /locus_tag="ACHA6Y_002281"
     CDS             complement(376..621)
                     /locus_tag="ACHA6Y_002281"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8988356.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(615..1016)
                     /locus_tag="ACHA6Y_002282"
     CDS             complement(615..1016)
                     /locus_tag="ACHA6Y_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8988357.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(1203..3011)
                     /gene="uidA"
                     /locus_tag="ACHA6Y_002283"
     CDS             complement(1203..3011)
                     /gene="uidA"
                     /locus_tag="ACHA6Y_002283"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM8988358.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(3034..4800)
                     /locus_tag="ACHA6Y_002284"
     CDS             complement(3034..4800)
                     /locus_tag="ACHA6Y_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM8988359.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(4813..5607)
                     /locus_tag="ACHA6Y_002285"
     CDS             complement(4813..5607)
                     /locus_tag="ACHA6Y_002285"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8988360.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(5617..>5988)
                     /locus_tag="ACHA6Y_002286"
     CDS             complement(5617..>5988)
                     /locus_tag="ACHA6Y_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8988361.1"
                     /translation="SSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSF
                     ARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFF
                     LYPLSKDKVLENCELLKQKHK"
BASE COUNT         1890 a         1186 c          734 g         2178 t
ORIGIN      
        1 cttttcgagt agctgttttc attatagcac cttccttact ttttttcatt tatttctatc
       61 agctgctttt gtcaaaagca cataggaatt tcacctctcc ccaagtttac ggcgagcctt
      121 acggaagtat cattatccca actttaaatc atcgcttttt attgactata aaaattattt
      181 tagaatattt atcgctcctt ctttacagca agaaatcatg gcgcaactaa aatatgctcc
      241 ttaaagtgtt aacgtactga tagaatctat ttaattttca atgaacaata ggttttctac
      301 ctaaaagaat ctactaattc cttaactatt agaagattct tttaaacaga aaaagtggaa
      361 aggggatttt tttaattagt taattttaaa ttttaagatc gcttctatta atttaatttc
      421 tagctgactt atcatatatt catctacata ggtaacgaat tgacctacct cgttaaatcc
      481 atttgtcaaa cataacttag ccatataaga atgataatgt ttgagtatca tcttgatcga
      541 aattggatct ccttctagag cagataagat aatggatagt ggaactaata ggtactgccg
      601 ctttttagat ttatctacca taggtcatac cccattttca attgttcaac tgccttcttt
      661 cgttgatacc agataccact tgtgctcata ttgagaattt taccgatttc agcatcatta
      721 aaacctgcaa aataataaag taggataatc tttcttctta tctccgataa ctgatcaatt
      781 gctgctgata acttttgatc tgaaattaat atttccattc caaataagaa atagacatct
      841 tgttcatcaa aatgagtttg ggaatacctg ccttcatttg aatctgccaa tggttcctca
      901 ttatccttta tgttagcgtt tcgttttcga atagacttag cttcgtttcg taatactttt
      961 ttacagtagg catcaaattg gtgttgtata gatatgtcat tgtaatgttg ttgcatgata
     1021 atatgctcct ttttccccct ttccactaat acaacaatca gaaagcccct ttttttcgaa
     1081 aaaactagaa aaaaaacata aaaatattta tcattcttta aacagaaaat ctttctataa
     1141 aaaacaaaaa aaaacgatta ggctaggcta atcgtttttt gaacattctt aaaaaatcca
     1201 aattattttt tataactgaa attgggtatt gatgaccatc gtttctgtag atagtaagca
     1261 atcattttag gttttctatc tctagtaaag attcctttct tatttccttg cactcttaaa
     1321 atcccttgac ttgttgcaaa atctgcaaaa ttccacactt gttcaccaac aaaattttca
     1381 catgtatcta ttacttcgtg atttgcttcg taataagcca cttgatactc ttctgtaaac
     1441 attgttggta ctgtatcatg aaatccagct actgtatctg ctccatattc tgtaaacatt
     1501 attggcgtat caggacatct ttccatccat ccatctagct cttttttcaa cagatatttt
     1561 gcatgtgcca gatcccctcc cgaaacatac caaccataat aacgatttaa acataaaaca
     1621 tcgacaatat ctcctacctt acaaacatct ggcgttgatt ttagataagt aacgattgtt
     1681 acaggtcttt tctgcggatc aagttcttta gttaattcaa ctaaaggttc aaaatattct
     1741 ttagcacctt cggaatccga atctggttca tttgcaatag accacattac aacacttggg
     1801 tggttcttgt cgcgttctat caattccttg atcaccgttt tatgagaatt ttctgttcgt
     1861 atctctttcc acgtattatg tttttccaca ttatcatcta ataaagtggc catgaaattt
     1921 aaatgtaacc ctactgcagg tacttcatca ataactacaa taccttctcg atctgctaat
     1981 cgcataattt cttctgagta aggataatgt gatgttctaa aagaattagc acctatccat
     2041 ttcattaaat taaaatcttt aatatttaca gcctcattca aaccacgtcc attaacatac
     2101 gaatcctcat gcttaccaaa tcctttaaaa taaaaaggtt tattattaat caaaaatttg
     2161 ccattggtaa cctgaacact gcgaatacca aattcttctg tataggtatc gatcacttta
     2221 ttatcatcaa ttaaactaat tttcatacta tataaataag cgtctagagg ttcccaaaaa
     2281 attggattct taagctgaat cattcctttc tttccttcag cttcagctac aagttgagcc
     2341 tcttcgtcat atatcgctat taagattttt ccggtccatt ctgtatcgat attgaattct
     2401 acacttgcat ccaaatcagt tattcttgta ttaatagtaa tatcacttat atagtctttc
     2461 ggagttgtat ataacttaac aggtctatga atacctgcat aattgaagaa atcaaaatta
     2521 actaagtttt ccttttttat atccccattt tcctgtactg tttccttcat aatccccaca
     2581 ggtaaagtac tatcgtcaat aatattgttt acagcaactg taattcgatt attcccgtct
     2641 tttacaaacc gattaatttc agactcaaat ggtgtaaacc cacctttgtg ttgagtaata
     2701 aactttccat tcaaaaaaac ttttgcttca tgtgttgcag atccaaaacg aagaactaat
     2761 ctttcttcta ataatctttt aggaagagtc acttcttttt cataccacac ccatccaaca
     2821 tgatttctaa tgttctctgt ttcaaataaa tcattataag aagagggaac tgaaatcgga
     2881 taactctctt tttcatttat tggttctaaa gcaagttctt cctgtaagtc atcatttgta
     2941 tctaatttaa atctccagag accgcttaag tcaattactt gcctcgtctc tgtcataatg
     3001 ggatacaaca tataaaccac tcctatctaa atattaaata aatgtatcct ttaaaccaca
     3061 ataagcatct attggactta ctcccttaaa ttcagaaaga cccattagtt tgtccaccaa
     3121 tatttctaaa gtttcttgtt ttccatcata agtattaata taagtcttta cttgaggtac
     3181 atcagccaaa tgaaacggac actgtacaga tacaaaaatt gttggaattt catgaatgta
     3241 aaacggtata tccggagttc ctttacttgc tggccagaca attcgttgaa ctgtgcctgg
     3301 attaacatta gctatattaa tgatcaagtc atatttttta gttaaatttt taattggtct
     3361 cttagcagca tatattccag ctatattttt aattttttct tcttgcgata atcctttcaa
     3421 ttcttcttca ggtgaaatgt atttttcaac ttcaaatcct tttgctgaca aaatctgttt
     3481 caataaatca atggcttgag gtctatttcc agcaactact tcaccaaacc caccttttac
     3541 cccatgtaca tctactagta aaatcctttt atatttttct ggtacaatag gccaaatatc
     3601 ttgtttatct ttaactaatg taattgcttg gtcagccacc tctttaaaaa ttgatttatt
     3661 ttcaattaaa ccaatttttt ccagagcttc ttgcttaggt aataaaattt gttcttttgc
     3721 ttttttgtgt aatcccaatt tagcttttaa ccctaaaatt ctttttaagg catctaataa
     3781 ccgatcatca gtgataattc ctttacgata gccttccatc atataattaa agtcttcatc
     3841 tggatcatta aaaaatagaa atagatcaca tccagctgca atagaagtgg gcaataattc
     3901 tgatcgtttc attgccccag tcattcctaa catatgacta gcatcagtaa ctactaatcc
     3961 attaaatccg agttttcctc taagtaagtc agtgattaat tcttttgata aagttgcagg
     4021 aagtaaatct tcatctttca gatcaggatt aaaatgtttt gaataagctg gcatatggat
     4081 atgacctgcc ataattgaag gtaatccagc ttctattaag cctgagtata cttttccaaa
     4141 agaattatcc cattcttccg tacctaaact attaatacta aaagatagat gctgatctcg
     4201 ttcgtcaata ccgtcaccag ggaaatgttt agctgcaggc aaaattccac tctcctgaat
     4261 acctttcata taagctaaac taagttctaa aactttatca gggttttgtc caaaagatcg
     4321 actagaaatg ataggatttc tccaatttct attaatatct acaataggag caaaactcca
     4381 gttacatcca atagccgatg cttcaatccc agaaactctc cctaattcat aagcatattt
     4441 cgcatcattt gttgcaccaa tttttacttc tacaccaact tcagtaccat ctgtacaagc
     4501 tccgttacca cctgcttccg tattagcagc aattaataga ggaattttac tttctgtttg
     4561 taagatatag ttttgatcat atacctcatc agcagttcct ggattatatc gtacagctcc
     4621 aatatggtac tgattgacca tattactcaa gtaattttta tcacgactag atcccatatt
     4681 aacaaacaat tgacctattt tttcttccaa agtcatagaa tcaatcgtct cttcaatcca
     4741 agaaatagca tcttcatcta aattatatgg tttttttcgt aaatctactt ttatagtcat
     4801 tttctttctc ctctacttat tagtaagatt tttccaaaaa gtatctatat atttttgttc
     4861 aatatctaca taatctcccg ataaacattt aaataaaaca tcagttctta agttttccca
     4921 tgattctttt tctttattga ccaaaatagg ataaaagaaa actttatttt tatttgcagc
     4981 ttctagatca ccaggagaat caccaatcat taatatctta tccaaatcac aaccattttc
     5041 aattacactt cgaataatat cttctttttt tcctttatct tgacaacaca gttcatcaac
     5101 taatgataat aattcatgtc tttcccattc ttcttgaaca gcttccttat tagctgagct
     5161 tacaataatt atcttcccat attttttcag agcttttata gtatctctag cacttaaaaa
     5221 gggtttatct aatccttttg cttgactaat ctgttgattg acttcttccg accattttaa
     5281 agcttttaat aaatcctctg actgagaata ttctatttcc ctcttcaaag atggattaga
     5341 caattctttt gttagagata cccagtgctt taatcgatta atcgatgtta catcattttt
     5401 ttctaatgtt ttaactaaac ctaaaaatct gtttattcca cgagtctctg aatataaatt
     5461 aatattattc caatcctgta ggaatttttc tctatgatga acatcaaaaa tctctgcagc
     5521 aattggtccg aaaaagagat tgtgtttata agtcatcgta tccataacac agccatctga
     5581 atcaatacaa aatatccaat tattcatatt tctctcctat ttatgttttt gttttaaaag
     5641 ctcgcaattt tctaagactt tatctttaga taatggataa aggaaaaata aaattaatcc
     5701 tacaatcaga aaacaaattg atggaattaa tgttgaagcg ttatataact tttctaaaac
     5761 atctcccgtt tgtacatttg ctaaagagtc atagcctata atagaaagag tccaacctcc
     5821 gacgcctcca gctagagctt gacctatttt tctagcaaaa gaatataccg catatacggt
     5881 cccatcttct ctttgatcag actttacttc ttgatcatca ataacatcag taatatttgc
     5941 ccaaataaca gtattaaaca cacccaaacc catatagcca accgaact
//
LOCUS       DAYYHX010000161         5980 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000161 DAYYHX010000000
VERSION     DAYYHX010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.162"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(325..2100)
                     /locus_tag="ACHA6Y_002287"
     CDS             complement(325..2100)
                     /locus_tag="ACHA6Y_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM8988362.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(2313..3215)
                     /locus_tag="ACHA6Y_002288"
     CDS             complement(2313..3215)
                     /locus_tag="ACHA6Y_002288"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM8988363.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            3494..5839
                     /locus_tag="ACHA6Y_002289"
     CDS             3494..5839
                     /locus_tag="ACHA6Y_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM8988364.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
BASE COUNT         1894 a         1179 c         1133 g         1774 t
ORIGIN      
        1 gtgtcgtctt tgtataggac actgtagctg tgtcccattg aaacatctaa tgcaagaatg
       61 taaggcattt gttttcctcc tttttaagtt ttgaaagaac tttactgctt catttcgatt
      121 ccaatttcct atacacgggc tcatggcccc aacatacgta aacctgattc taaaatgaca
      181 gtaaagaagt tcggtttttg atagggactc atagtcctca aggctttgtt cgaactttct
      241 ttcactcttc actataatct aaaaatagaa aatagaggag aacatcattc gtcaatgatg
      301 ttctcctcta atcttagtat gtttttattt ggaagataaa gcttttttca gattcaattt
      361 actgaaaact agcatgcttg ttactacgat gaattgcgtt aatggcacgc ctgcgccagt
      421 gtagacactt aaaaaataat cctttttttc tggcgcataa gaacaaaacc acgagacgcc
      481 gaaaagaaaa accaattgtg tcagtattaa tccggtgact acaatcaaag aggaactata
      541 ataggcacga tcagcttttg caaatgcggt tcctagtatc ataagtaata tccctgctat
      601 tgaagcaatc agcacgggaa tggccagaac ttggtaaatt tttatcatga cattcgcgac
      661 ttttgctgtt tttgtcaact ggaaatcagg tgttctcgga tcactgctat cgatccaatt
      721 atccattagt ggaagattca ataattcttg ggcctgttcg acttgttctt gcgtaccgaa
      781 actataattc cccccttgat ggaaatcttt gtatcgccat gcataggcat atcctgattt
      841 cagaaaagca aatacgattg gcagatcatt catcgtcttt ccatcccctg atcccatcag
      901 atagaattct tttttctttt tgatagttcc cttttgatag gcttcttcta attcttgatt
      961 gacttttttc caaaaatcat tggtttcttt tgcattgttt tggtaataac cagattcgct
     1021 taccacatct cgcaatgccc aaaaaatgat atctcctggt aattctttta cttttccacc
     1081 ttgggtccac gcatgagtcg tatacaattc gtcaatacga gtagaaacag catgaaatgt
     1141 aggagatgcg tctaatgctt tatttaatgc agcatgcgag acccatatct cactgttttc
     1201 tttatttaag ttcgtttcgt catctattcg gatcagctgt ttcatcaaac gtccaaattc
     1261 gccactcgaa cggtcattga tggcggcaat actatatgtt gtctcgtttt tatattcgat
     1321 ccactgatag acgatggaga aaccgactag agggagtaaa aacaatgaaa gtttcagcca
     1381 ttcttgtttc gaaaggagaa atggtcgttc ttttaacttc tttgcttctt tgaaagaaaa
     1441 gtcttgtcct agcaagatct ggacgaaact gatgcctaac gcgactacag taaaaggaag
     1501 aagccagatg gaatcttcgc gaatgaacca aaagaaagga aaagaaacag ctagccctaa
     1561 aaaccacggc agcatttttt tgctggaact atatttacgg taataaatag ctagtaaaca
     1621 agcgataatg atctcaacag ccggaatcac gagggcatta cgatacgttc gagtggaata
     1681 ttcatgtgta aaactaatcg gagaaaacaa aaaataaaaa tagaaactta aaagaagata
     1741 cttgttcttc acgatcggtt taaacgcgag tgcaaccaat atactggcaa aaatactgaa
     1801 catgccgaac aaaactgcat aagaaagatg cagtttatgt gcaagtacta aaaataagct
     1861 gtaggagatt cctttggaaa gtgttgtagt cgtatactcc cctagccatt taccgtccaa
     1921 aatattttga gcatagcgaa tcagcagctg gtcatcatag gttgccgaca tgtctgttcc
     1981 atagattgct ttactatata aatacaaacg aataattgta aataataggg ctatgccaag
     2041 aataaagtat gtatgtttct tttttagttg gttgatcaag ggttttaaag tgttcagcat
     2101 cttttcgtcc ttccaatcac gttttgccac ttgcttgtga aacttttcgt ttttttactt
     2161 ttctaccagt ataccctgtt tgtctaaatt taaaaagagg ggggagaaga tgcatgatga
     2221 aaaagactgc aaaaagcact cctttgtttc ttaaacaaaa aaggacaagg caatgtgcct
     2281 ggtccttttt tgctgctgtc agatagaaaa tcttagagta tcacttcttc tgctaataac
     2341 tcaggaaaca cttctggatc gaatatttgt gctttgaaca tggcgatttc ttttccatat
     2401 ggtgcgtaag ctttcttttt atctttgccg acatagggag tttccaagat tttcgaaata
     2461 ttgtcaaaca ttggatcatg gacgatctta ttcagcgtgt cgaatccgat tgttccaaaa
     2521 ccaatattgg cgtgccggtc tttatgcgaa ccttgcggat ttttagaatc attgatatgg
     2581 agtactttaa gccgatctaa cccgatgatc ttatcaaact cttccattac acctgaaaag
     2641 tcttctttaa tattataacc ggcatcattc gtatgacagg tgtcaaaagt aacggataat
     2701 ttttcattca attttactcc atcgatgatt tgcgctaatt cctcaaatgt tcgaccgatt
     2761 tccgtacctt tacctgccat tgtctccaaa gcgatttgtg ccgtctgttc ttttgtcaaa
     2821 acttcattca agccttcaat aattctagca attccggctt ctgttcctgc ccctacatga
     2881 gcacctgggt gaagtgtaat ttgagtagcg cctaaagctt ccgcacgaac aatctcttct
     2941 ctcagaaatt ggacggcaaa aggaaacatt tctggtttga tcgtattccc caaattaatg
     3001 atataagggg catgaacgac gatatttgtt agaccgtttt ctgccatgta cgcttttccg
     3061 gcatcaatat tcatctcttc gatggctttt cttctagtgt tttgaggggc gcctgtatag
     3121 atcataaaag tcgaagatcc ataactggct gcttcttctg ctgctcctaa cagcattttc
     3181 tttccgctca tgctgacgtg tgagcctaat aacatggatg gattcctcct tgaaaggcaa
     3241 ttgttactaa caattcctat atattggtaa gatgatatac aaagaaaatg attgattttt
     3301 ctttgccctg ttctccatag taactgaaat tttctctaaa gaaaagctta agaactcttt
     3361 tataaaaaag atcattttct cacgttttaa ctcttttaaa aaagaaattc ctgccaaaaa
     3421 cagccttgtc tgtttttttg ttctattatt tgctataatt agcgaaataa aaccattagt
     3481 tgaggtgacc cttttgccac gcaaaaatac tcgaaaaaaa cagaatagaa gaaaaaaaga
     3541 aaaatggttt gttcctaaga ttatttttag agtttttcaa tcattgactg tttttattac
     3601 tgttcttctt ttgatgttcg cagctctagg tattggaata ggcgctggct attttgccta
     3661 tctcgtcgaa gacacacaat tgccgactaa aaaagcactg cagacagaac taggaaacat
     3721 tacagaaacg tcaaagattg tttatgcaga taatacagag atatcaaaaa tccagacaga
     3781 tctgatgcgc acgactattt catctgataa aatttcacct ttattgaaaa ctgcaattat
     3841 cagcacagag gatgaatact tcgataaaca ccaaggttac gttcctaaag ctgttttacg
     3901 agcattagta tctgaagcta ctggtattgg ctcttctggt ggatcaactt tgacacagca
     3961 attagtcaaa cagcagatcc ttacagatga aacaacattc aaacgtaaag caaacgagat
     4021 cattctttct gcacaagtag aaaaatactt ttcaaaagat gagatcattg ctacgtattt
     4081 gaatgtctca ccatttggtc gtaataataa gggacaaaac attgctggag tccaggaagc
     4141 tgcaagagga atctttggtg tagatgcaaa agatgtgacg ctgccgcaag ctgcttatat
     4201 cgctggatta ccccaaagcc cgatcacgta tagtccatac acgaacacag gtgcgctaaa
     4261 agaagatctt tctgccggcc ttgccagaaa agattttgtt ttattcagta tgtatcgtga
     4321 aggccaaatt acgaaagaac aatacgaaga agcaaaagcc tatgacctta caaaagactt
     4381 tctaccacaa caaatcgcag aacagactga cagagaattc ttgtattata ctgtgatgaa
     4441 tgaagcaaca agaatcattg cacaacaatt ggctgaaaaa gacaacgctg atatgtctga
     4501 ttcatcggtc agtgacgctt attatcaaaa ggcacagcaa acgatccaaa ataaaggata
     4561 tacaatccat tcgacaatcg ataaagatgt ctatgctgca ttgcaaaatg gcgtagaaaa
     4621 ttatggatac atgttagatg atggtgctgg cagtcaagta gaaaccggaa atgtattgat
     4681 ggataacaga actggccgca tctacggatt cgttggcgga cggaattaca gtcagaatca
     4741 aaataaccat gccttcgata cagaacgtca agctggctca tcaatcaaac cagttcttgt
     4801 ttatggacct gcaatcgaca tggggcttat cggatcagaa tcccgtgtat cagattatgc
     4861 aacaacttgg caagaaggag aaaatgctgg cgagaaaatc gtcaatgcta ctaacgaagg
     4921 atcgaacacc ttccaaaccg tccgtgagtc actagaatgg tccaacaata tcccggctta
     4981 tcacttatat caagatgtgt tgaattccgg tggatcgaaa caatatgctt acgagcacta
     5041 cttggcaaag atgaactacc cggcaaatga caattggggc gttgaatctg ctcctttagg
     5101 tacagtagat gtcacgacgc ttcaacagac caatggattt caggctttag caaacggcgg
     5161 cgtctatgaa gaaggctata tcatcgattc catcacagat aacgcaggaa atgtaattta
     5221 taaacacgaa agcaactctg tccggatcta ttcggaagca acggcatcga tcatgaatga
     5281 catgatgcgc agtgtgatca atgcaaaaat cactacacca ttcaaagatg ccatctcaag
     5341 cttaaacgga aatctaggaa aagcagattg ggtcggaaaa actggttcta ccaatgaata
     5401 ccgagattca tggctaattg tctctactcc gtctattacc atcagtagct gggcagggca
     5461 cgatgacaat actggaatgg acagcaaagc aaggatacgt tcggctaatt atcttgccaa
     5521 tctgatcaat caagcttacc aagcaaaacc tgacattttt ggcactgatg agaaattcga
     5581 attatcatct gatgtgatga agaaaaaagt atctggattt actggtcaaa cacctggaaa
     5641 agttactgtc aataaaaaat ccatccaaac accaggaaaa acagttgaat cgctatgggc
     5701 gaaaaatgga ccgaagaaaa gcgcctttaa atttggcgtt ggcggtaccg atgaaaacta
     5761 tacagattac tggaacaccg cttcagctta tgcaagagcg aatcaagcaa ccaaagaatc
     5821 agatgagaaa gatgattgat ttaatagaat acacagtaat aagggactgt gacaatagta
     5881 ttgtcacagt cccttatttt tttgatggtg gtgaacataa tgcagcatct atacacggat
     5941 tgttttatat acgataaatg taatttcaac cactttctct
//
LOCUS       DAYYHX010000162         5954 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000162 DAYYHX010000000
VERSION     DAYYHX010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.163"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..813
                     /locus_tag="ACHA6Y_002290"
     CDS             <1..813
                     /locus_tag="ACHA6Y_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFM8988365.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACHA6Y_002291"
     CDS             786..1229
                     /locus_tag="ACHA6Y_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988366.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2399..3367
                     /locus_tag="ACHA6Y_002292"
     CDS             2399..3367
                     /locus_tag="ACHA6Y_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8988367.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3384..3908
                     /locus_tag="ACHA6Y_002293"
     CDS             3384..3908
                     /locus_tag="ACHA6Y_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8988368.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            4284..4565
                     /locus_tag="ACHA6Y_002294"
     CDS             4284..4565
                     /locus_tag="ACHA6Y_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988369.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4567..4983
                     /locus_tag="ACHA6Y_002295"
     CDS             4567..4983
                     /locus_tag="ACHA6Y_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988370.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            5613..>5954
                     /locus_tag="ACHA6Y_002296"
     CDS             5613..>5954
                     /locus_tag="ACHA6Y_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFM8988371.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2258 a         1018 c          965 g         1713 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatctcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacggaggta tttggctagc aaatatagag cttgccaaac
     1441 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1501 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
     1561 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
     1681 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
     1741 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
     1801 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     2041 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     2341 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     2401 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     2461 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     2521 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     2581 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     2641 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     2701 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     2761 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     2821 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2881 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2941 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     3001 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     3061 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     3121 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     3181 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     3241 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     3301 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     3361 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     3421 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     3481 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     3541 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     3601 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     3661 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     3721 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     3781 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3841 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     3901 ggcaataaaa ccagcaattt aaattaataa aaattgctgg ttttattctt ctttttgctt
     3961 ttgcgagcag agcaacatgg acccatgttg cgatattttg gtggcgcccc aatccccgtt
     4021 ttttcgttga atcttactcg gactgcactc gttttacagc tgatttttct gaattttgaa
     4081 agagcataag gaaacgtggt atcactttct tacgaaaggt gctagatagg ctgtgcctat
     4141 cacgtttctc agaaaaaaat ttgctatcac ttttttgcca ttgtggctag gctatgtata
     4201 gtgttttggc gaaaaatagc tatacaaata ctatatttgt atggtataca taaattatac
     4261 gctgatacga aaggaaaatt tccatggaca ttttagttcg aaatatcaat cccaaaatta
     4321 ttgcaagaat agataaacgt tgtcaggaac tcacaaccaa aacgggcaaa aaatggagtc
     4381 gaaataaata tctaaaagtt ctaatcgaaa gtgattttga ccgtcaactt atgaattaca
     4441 aaaaagatca attcgattta ttactcgaaa aatttattgc cattcaaaca gaaaatacga
     4501 acatactgaa tgactatgtt cacacaaaca accaactcat tgaaacactt atcggaggaa
     4561 tataacatga gaaaaacttc tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac
     4621 gttttagatt ccatcatcga aaaaaatcca gacgtcaaca attattcaga agccattcgt
     4681 tttttatgta tgagttatga acctgatacc aatacaacca caaccagtaa ccaagcaaaa
     4741 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
     4801 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg
     4861 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
     4921 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
     4981 taaaaaaaga aaccgatccc taaaaaaatt cctcgatttc tttttaggga tcagtcttcc
     5041 agcaagattt gcaagttggt accaacttgc gaaaaaaaag aaaaatccgc ttatgcaact
     5101 tcttcttttt gtattttggg gcgctacccc aatccccaag tatcggttat ttaatagcgc
     5161 ggaaatccaa agaataggac gtaccctgaa acaaaaaaag actgctctca cgagcagccc
     5221 aaaatgagat tatcctattc atcaacataa aaatattaca aagtgaagaa ctagagaaag
     5281 acaaaaaaag catatcaaat cctctcaaaa ataacaagtc tttaaaaaat gacagataaa
     5341 aatgcacaaa gaactaaaaa agagaaatcg cgtaaaatca agcattagaa gctagtaact
     5401 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     5461 ggtctagtcg acattatgtt aactagctcg ataaataacg attatgttcc caaaacaata
     5521 ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt atataattat
     5581 aggtgtatac cacttaaatt taacacaaag acgtgattat tttgaaaccc aaaaagaaaa
     5641 tttatctagg tctcctcttt tccttgatga ttggaatttt tagtatagga attacaaata
     5701 cctatgcttc agaaaattct aatttcaata tcaactccca accaattgaa gaacaagcaa
     5761 ttaattttgc gaaaactgat tttgaacact ccataacttc aattctttta atggaaaata
     5821 acaccgaaac tgatattaac tatactcttg gttcgccatt taaaatgctc gatggagaaa
     5881 atacggcaaa tagttctatt ttctttccag tattttcaga cgctgataat acgattaaat
     5941 atatttatac cgtc
//
LOCUS       DAYYHX010000163         5946 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000163 DAYYHX010000000
VERSION     DAYYHX010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.164"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..990
                     /locus_tag="ACHA6Y_002297"
     CDS             <1..990
                     /locus_tag="ACHA6Y_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFM8988372.1"
                     /translation="LETSYEFRRNILLSYMFKKDLSEAFNCLSRQLVNQFNTLKSNEE
                     ELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCIFGRYSLDEDGL
                     IFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVEITFGKETLEEN
                     LQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGKQNGFKALMYLH
                     RYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTKEIVKIQKQLKE
                     VREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKGVKEPKK"
     gene            1106..3628
                     /gene="pglZ"
                     /locus_tag="ACHA6Y_002298"
     CDS             1106..3628
                     /gene="pglZ"
                     /locus_tag="ACHA6Y_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFM8988373.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            3746..5779
                     /gene="brxL"
                     /locus_tag="ACHA6Y_002299"
     CDS             3746..5779
                     /gene="brxL"
                     /locus_tag="ACHA6Y_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFM8988374.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
BASE COUNT         2059 a          820 c         1197 g         1870 t
ORIGIN      
        1 cttgaaacgt catacgagtt tagaagaaac attttgttaa gttatatgtt taaaaaagat
       61 ctctcagagg catttaactg tctaagtagg cagttggtta atcaatttaa tactctaaaa
      121 tcgaatgaag aggaactgaa cgacatattc attaatcatt attctcttga aaaagaatta
      181 acaaaagagg ttcaagatga atatatctct gcaaggttac ctgataaggc gagggaaatt
      241 aaggatttta tttcttattc aataggttgt atcttcggtc gttactccct agacgaagat
      301 ggtctcatct tcgctggcgg cgaatgggat ccttcccgct atcaaaaatt taaacctgat
      361 actgataatg ttttactcat caccgatgat gagtatttca aggatagcca aattgacatt
      421 gtcaatcgtt tcattgattt cgttgagatt acttttggta aagaaaccct tgaagaaaat
      481 ctccagttta tcgctgacac tttaggtggc aaagggacaa gtcgagaaat tattcgtcgt
      541 tacttcgtac aagattttta caaagaccat ttaaaaacat actccaatcg accaatttat
      601 tggcaattgg actcaggaaa acaaaatggc tttaaagcgt taatgtactt gcatcgctat
      661 acaccagatc aattaggaaa agtacgtact gaatacctac acgaactaca aaaagcgtat
      721 gacaatcgaa tcgaattgcg tcagcaacaa atttcaacaa gtgataaccc aaaagaaaga
      781 aatgcaatga caaaagaaat tgtaaagatt cagaagcaat taaaagaagt tcgtgagtat
      841 gacgagaagt tgggtcactt agcattaaaa cgaattgaat tagacttaga tgatggtgta
      901 attgtcaatt atgataagtt gcagcgtgat cctaaaaccg gcgagaaatt ccaaattttt
      961 gcaaaagggg tcaaagaacc aaaaaaatag ttatctaaga aagttgtttt ctaacaactt
     1021 tcttttttat tcttaaattg cgtgcgaaat tatccaaatt atgtcaatat aaggggtata
     1081 gaaagagaac gattaggtga gattgatgac agatataaat acgcaacaaa ttgaagaaaa
     1141 attaaatcaa cgttttccta attatggatg taggaaaatc attttttggt ttgattcaaa
     1201 tcaggatttt cttgaagaaa ttgactcatt ggtcattcaa aatgcaaaaa tctatcgatt
     1261 agaaaaggac gcacaattta aaactaagcg tttattggaa tatgaagatc ccgaaactaa
     1321 ctatttaatc tatgcgccat ttcctcgttg ggatgataaa gatgaagata atcatttgtt
     1381 gagtgtattg aattattcag aggaatttag tgcagaccgc ttagcgatta tcatgaatga
     1441 attggctatt ccacttagat ttcatcagac agttaaacag tatcgtaatt tctttaatgc
     1501 aaaggatcgc atgagaagtt ttggtgaatt tagtacgcat atgacaatta ataaatcaga
     1561 aacgattgaa ttaatgataa tggctgtttt gttgaaatcg aaaagtattc gattaaatga
     1621 tttactgcgt gtagttctaa aaagctttac agaaagtagg gagaatcctt taagggaact
     1681 tgataagtat cagttaacag ctaaattttg ggaatttatt caaatgaaat ttggctttac
     1741 ggatagccag ccaacattgg aaaagctgac gatgtgtctt tatttgaact atttttatta
     1801 tcaaattgat caagagatgc cgaagaagtt caaaggattt gaaatactac atttgaaagc
     1861 aaacattatt actttcatgg atcagtttat gaatgataca cgttacatgg aaagctttga
     1921 acagctatct aaaaaagtat atcaggctat tgatggtgat ggattattag acagtataga
     1981 aattgatgat ctaattgaag cagacgtatt ttctgagatt catgcaaaaa tattaatgtt
     2041 ttatgcagaa cgattaaagg cttctgacac tacaacgaaa attgcaggac aaacaatttt
     2101 tgatagttta aagcagcgcg aaaaaatgca tttcgcatcg gattatattt atgagtatca
     2161 attattgaaa catgcttatt atcttgtgga taaaaacaga ttgtgcttgc cgatgaacat
     2221 ggataatttg ctaactcaat atgagaagga aatgtattgg gttgatacgc attatcggaa
     2281 atttatttgg aatagtgatc atatagaaga aaagcaaaaa tatggccaat tgtatcgatt
     2341 agttgaagca aattattcga agtttcttga tcaatcagga caaatttgga atcaaacttt
     2401 tgaatttaag aatcgtccat ccattcgaaa attttataat caagctgtaa tgaaacgctc
     2461 tgttaaaact gtagttatta tttcagatgc ttttcgatat gagttaggga tgaatttaga
     2521 acgtaagtta gttagagaaa agaagtattc aacaaacatg gctagctttc tttctgtatt
     2581 accttcagtt actgaattcg gtaaagccgc cttattacca aacaatcaac taacttacaa
     2641 tgaaggaaca gaagtactga ttgatgggaa aaaaacgaat ggtttatcca atcgaaatgc
     2701 gattctccag caacgagatg caaatgctgt agctttaaat tatgatgatg tggttaagat
     2761 gaataaggct gaggtacgag aagtatttaa tggcaaatca gtcatttata tttatcacga
     2821 tcaagtggat agaacaggtg atcactcgaa tgaaaatcga gtttttgagg cctgtgattt
     2881 agcgataagc gaaatttccc gacttattaa tcgcttgcat aatgatggca gtatctatcg
     2941 ttttatagtt acggctgatc atggatttat ttataaaaga agccacgttg aagaagtaga
     3001 taagattgaa aatccatcta gaaatgaaaa agatcgtgta gaacgtcgtt ttattcttag
     3061 tgaagaagaa tacgaaataa ttggtacgaa aaattactct ttgggagatt ctttaggaaa
     3121 tgatgacact agaaacattt gtgtcccgat tacttcaagt atttttaaga aagctggcgg
     3181 gggacaaagc tacgttcatg gagggtcatc tattcaagaa atgctagtcc ctgttctaga
     3241 aattacggta gccaaaggta gcaatgcaaa ggaaccagta gcaattgaat taatgactag
     3301 caaccgcaga attacaggat tatcaaccac gttagaattc tatcaaaaaa atgcaattag
     3361 tgatgtttat gaccccgcat ctttctggct ttattttgaa gatgaatatg gtgaacgtat
     3421 ctcaaatgaa gaaatctatg ttgcggattc aaaaacagaa gatgcaaatc agcggtttac
     3481 taaatttgtt tttgaatttg taaatcgtcg atatacagtt gaagatactt attatttagt
     3541 gataaaaaat caaaaaaatg atttgaccga gcgaataaaa ttcattatag ataatccttt
     3601 tgcagaatca tttgattttg atatataaaa acgaggtatc cacaattttt tgtggatact
     3661 ttttcttttg tattatcaag cctactggtt aagtatgata tactttaatt gctattataa
     3721 tgactgaaca catggaggaa ttctagtgga acaacaggaa attttaaagt tatctgaatt
     3781 ctttcccgga aagattgtca gaaaagattt aacaaagaaa attaaagaag gtgcgaatgt
     3841 acctgtttat gtattggaat atttacttgg tatgtatgct tcgtcattgg atgaaacatt
     3901 ggttgaaaaa ggtgtggaaa atgttaaaaa tattcttgct cgaaactatg tgcgtcctga
     3961 tgaagcagaa aaagtaaaag caaaaattcg tgaattgggt ggttatacag ttattgataa
     4021 agtaactgta catttaaatg aaaagaaaga ttgttatgaa gcagaattct ctaacctggg
     4081 attaaaaaat gttcaaatta gtgaagagta tccgaaaaaa tttgatcgat tattaggtgg
     4141 ggggatctgg tgtattgttt cgatggaata ttggtatgat gagaatgatc gttggaatag
     4201 cccttttatt gcaaaaaagg ttgatccaat ccaaatgcct ggtttagaca ttgaggctta
     4261 caagatggct cgcaaggagt ttactacaaa tgaatggatt gacttacttc tacggtccat
     4321 tggtatggaa cctacacaat ttaataaaag ggtaaaatgg cttcatttag caagacttat
     4381 tccattaatt gaaaataatt ttaatctctg tgaactagga ccacgtggta caggtaaatc
     4441 gcatgtatat aaagaaattt caccaaactc aatccttgtt tcaggtggac aaacaacagt
     4501 agcaaacctt ttctataata tggctagtcg tacaatggga cttgtaggta tgtgggactg
     4561 tgttgctttt gacgaagttg ctgggattaa atttaaggat aatgatggcg ttcaaatcat
     4621 gaaagattac atggcttcag gatcttttgc tagaggcaaa gaagaaaaga atgcttcagc
     4681 atcaatggct tttgtaggaa atattaatca aagcgttgat gtaatattga aaacttcgaa
     4741 tttatttgag ccatttccgg aggttatggg gacggattca gcctttttgg atcggatgca
     4801 ttgttattta ccaggttggg aaattcctaa atatcgacca gattttttta caaatgacta
     4861 tggcttcatt acagattatt tttctgaagt catgcgtgag cttcgaaaag ttagttttgg
     4921 cgatagtttt gataaatatt tcaaacttgg ttctcaatta aatcagcggg atacgattgc
     4981 agttcgtcgg attgtatctg ggatgatgaa actgttgtat cctgatggta gatttgaaaa
     5041 aagtgatgtc gaagaaatac taagatttgc tcttgaaatg cgtcgtcgtg taaaagaaca
     5101 attaaagaaa attggtggaa tggaatttta tgatgtcaat ttttcataca ttgatttaga
     5161 agaagagcga gaagagtatg tttctgttcc tgaacaaggt ggaggaactt tgattccaga
     5221 agggttaggg aagccagggc atttgtacac ggtatcttat ggcaaaactg ggcaattagg
     5281 tacctataaa ttggaaacgc aaatgacttc gggaaatgga aagtttgaac gtactgggat
     5341 caattcagat gctggagcaa gagaagcgat ggattcggca taccgatatt taagagctaa
     5401 ttatggaagt gttagtcagt ctattagttt gacgacaaaa gattttgtcg ttcatgttca
     5461 agatatgaat ggcattggta tgacaaaaac attatcgtta tcaactataa tagctttatg
     5521 ctcagtttcc ttaaataaac ctgtattacc aagtttagtt attttagggg atattacaat
     5581 tggtggttct atgattaagg tagataattt agctaactgt ttacaagtag caaaagatgc
     5641 cggagcgaaa aaagtacttg tacctatgat aagcgctgct gatattggaa ctgtaccccc
     5701 agaattaatg ggtaccttta atttgatttt ttatcaaacg ccggaagatg ctgtctttaa
     5761 agcccttggg gtggaataga caacttttcc atttgctggt ttgactacgt accaatctat
     5821 attacttact agattagtcg ttaatctatt cggatcgaat gggcaaaata tttttgtgta
     5881 tgtccgtcct tgatcatgat tccaaaaaga atcattgggg gctgtggcgt aaatattata
     5941 ctataa
//
LOCUS       DAYYHX010000164         5871 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000164 DAYYHX010000000
VERSION     DAYYHX010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.165"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACHA6Y_002300"
     CDS             <1..1152
                     /locus_tag="ACHA6Y_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFM8988375.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACHA6Y_002301"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACHA6Y_002301"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM8988376.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACHA6Y_002302"
     CDS             2728..3003
                     /locus_tag="ACHA6Y_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM8988377.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACHA6Y_002303"
     CDS             3009..3776
                     /locus_tag="ACHA6Y_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM8988378.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACHA6Y_002304"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACHA6Y_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM8988379.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
BASE COUNT         1996 a         1042 c         1314 g         1519 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacattg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa t
//
LOCUS       DAYYHX010000165         5817 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000165 DAYYHX010000000
VERSION     DAYYHX010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.166"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..344
                     /locus_tag="ACHA6Y_002305"
     CDS             <1..344
                     /locus_tag="ACHA6Y_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8988380.1"
                     /translation="LKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAEKEQKI
                     QLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALELI
                     ELQISEGIDKQ"
     gene            420..638
                     /locus_tag="ACHA6Y_002306"
     CDS             420..638
                     /locus_tag="ACHA6Y_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988381.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            625..3303
                     /locus_tag="ACHA6Y_002307"
     CDS             625..3303
                     /locus_tag="ACHA6Y_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM8988382.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3294..3491
                     /locus_tag="ACHA6Y_002308"
     CDS             3294..3491
                     /locus_tag="ACHA6Y_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988383.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3495..4106
                     /locus_tag="ACHA6Y_002309"
     CDS             3495..4106
                     /locus_tag="ACHA6Y_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8988384.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4091..5482
                     /locus_tag="ACHA6Y_002310"
     CDS             4091..5482
                     /locus_tag="ACHA6Y_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8988385.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
BASE COUNT         2210 a          818 c         1147 g         1642 t
ORIGIN      
        1 ttcttaaaca aatatgggga gaaagtattg tgaattgctt aaaactggct agcaatagtg
       61 attatcgtca attcgacaac tggtacaaaa aattcaaata tgcgattaga tctgctgaaa
      121 aagaacaaaa aattcaactg aaaattatct atgaaatttg taataacaag tattttgtag
      181 atcatgttag agaacagcta tcaatgactt tacgtgactt gatacgaaga gcaaaaacag
      241 atcatcgtat caaacaaaaa gacgattata tctttgcttc tctaaaaaat aaagcgttag
      301 aactaattga attgcaaatc agcgaaggaa tagataagca ataagaatag aattgagtca
      361 aatgaaatgg aggggtatct ccacttcatt tgagaacttt gaatatagga ggggttattt
      421 tgattaaaaa tcgtttaata gatcaagttt ggtgtctaga tcaaaataca gatagtctcg
      481 aattaatcga gactatttgc ttcaacacta tagttctcga tttaagagct gaaaatgata
      541 attgtgtaac tcatgtcata attaatcaaa aaatggctca gaagctctct gagagcctca
      601 gaaaatgggc taagggaggt aacgatgaaa ataattgaaa aaaagattgc caataaaaaa
      661 ataaattggt tggttgttaa ttttcttgtt ctgatagcta tgattatttt tacaagtaat
      721 attgctcatg cgagtatttt caattggttt gatgacacaa aaagtcaaac agagtttttg
      781 aataattctc tttatagacc ttacctaaaa aagtacaatg gagatttcct tagtcagttc
      841 gggatttggc tcggatgggc tgtggtaaaa ggcatgttta ctgtgacaga ttcgattcaa
      901 aatatgattc cagatgtgct ggatttgttc aatttcattg aaagtacagg gctaaataat
      961 gtttatcaat cagccgtaaa tacaattgtc attggattaa tgatcctttc attgatgttt
     1021 gttggttaca aaatgattac tggaaaagga acagtggatt taaaatcggt tggaatgaac
     1081 gtagtgatgt ctatagcatt aatactttta atgccaacca tgatttctag tggtattcaa
     1141 tttgctaaaa cattttataa ggactctact acgattacaa attcagatga cggagtcgct
     1201 tggtcattga taaaacaagg agtaacagat ttagcatata ttaataaaac agatcagtac
     1261 tcaagcattg ataaaacgga ggatcgaaat aatttaacac gaaaaaattt tcaacagacg
     1321 gatctcacac aagtgttaac ggataaagtg attgataaat tggaaaaaga aaatccaatt
     1381 gcggacaatc ttcgttatga attagttgaa aattctaata atgaatttgt tgctacaaag
     1441 ttttctgaca acttcttatc aacattttct gatagcttga aatcaggcta ttaccgatac
     1501 caagcaaatc tatgggggat ttcaattggg ttgacagcgt tagcaatcgc ctatgtgttc
     1561 agcgcgtttg tgatcattac agcaatacta gaacttgcat ttaaacgtgt gttaggtgtt
     1621 cttgtttttg caacagatat tgagacagga cagcgttcaa aagttgttct atcagatatt
     1681 ctacaatgtt acttaactgt tgggtttcaa ggatttgggt tatcaatgtt tgctatgttt
     1741 atcaattttc tgaactcagg acaaggtatc tcaacaaata tttttataaa aacaattgct
     1801 tatatctgtg ccgtttttgt tttaatcaaa ggcagtggaa cagtcatgag atactttggt
     1861 gttgatattg gactcaaaga aggctatggg caacttgctt ctgcttttgg aatgggcgca
     1921 atgctgttta gaaaaggatc aaatggattt aatcgtgcta aaggaagcgg agaaggagaa
     1981 gatcgaaaac cagaaaagaa tttcggcgaa actctatcaa aaaaagctgg caaaactgga
     2041 agagctttgg gatatgccca tgaacgtgga ttatccggtt tagcttcaga tggagctaca
     2101 atggctagtg aaagagcaac aaaaccattt aagagtatgc gtgatatagc taatgatacc
     2161 aagaataagt ttaaagaagg tttagatgat ggtacagtgt ccgctattaa taaaaatagc
     2221 aaacctatgt tagctaaaaa taaagaagat gaaactggaa aatatgctga ttccgtgcca
     2281 acacgtcttt cagatagaaa agatggtgct aaagttgaaa atgccgatag aattatgtct
     2341 agttctgaac gtatgcgtga agcaatgaaa aataatgctg aatcgaataa taatcctgca
     2401 agcgcaattc agcaaaaagt ccaacaagat attgaagaac gtaaaaatgc tatgcacggt
     2461 caagctaagt ctgctgaaga attaattaat cagaagcgtc atgaagcgaa atacacgcct
     2521 gaagcaatga atcgcgagga aataatgtta aggaaacgtg tagaagggcg gacaggcgct
     2581 aatattgacg gaaaagaagc gttgaccaaa gaaagaattc aagaagctaa aaattcaaat
     2641 acaggattag aaaaattagt caaagaaaat ccacaaaatt ctacaagtag tcatggtggc
     2701 cgatcagtag atgtccgaga aaatattcaa ggttcactga atggtcatgt tggacggaat
     2761 gttgatgtaa gagagaattt acaaagttct tcaaatgctc aaggtactaa gacaattgat
     2821 gttcgagaaa atctgcaaag tagcagtaca agtcaaccaa aaacagtaga tgttcgagaa
     2881 aatgtacaaa attctacaag tagtcaaggg tttaaatcag tggatgtccg agagaatgtg
     2941 caaggtagca gtgcaagtca accaaaaaca gtagatgtcc gagaaaatgt acaaagttct
     3001 acaagtagtc aaggattcaa aacagtagat gttcgggaga acattcaaaa agatagtgga
     3061 gaaatgaaag aaaaaacaca aaaaatcaat atcgttgaag acagaaagtc agcgaaaaaa
     3121 tttgattcaa atcatgaaac agtgattatt gatagtgaaa tccgtgaaaa tgataaggga
     3181 tcaaaatcaa gacgaagatt cacttatgaa gacaatgagt tattcagaga tacacttaat
     3241 gatcctaatc cacttttcga taatttatta agaaataaaa gggggggatt gtcatgcaat
     3301 taattctaaa gattagtacg catgatttct tagaacttga cgaattatgt gaatcagtaa
     3361 agaacgcaac aaatgaaaaa atcacggtca ctaattatat tgaactgatg attcaagaag
     3421 aattaaagat gaaaaatgaa atagagagca atttcaaatg ttgctctcta gaaaaaaatg
     3481 tgaggggata atcaatggat gataaacaaa aaatgtacga aaaaaatcgc aaacatagtg
     3541 aggccttaaa ttttcgtgtc gataaaaaga cttatcttga actgacattg attgcaaaac
     3601 aaaggggaat ttctagagca gacgtttgcc gtgagcggtt aaagggactc aaatatgaac
     3661 cgccgttgtt agctgaagat gatagggtag ttttaaccaa acagttaaat caaattggta
     3721 acaatttgaa tcaagcggta aaggcattaa atatcattgc taaatatttt agagaagaat
     3781 ctgaagataa aaaagaaaag agcctacatt ttagttttga tgctagccgt tacaacattt
     3841 ttaaagatga acttgcgaat agcatagcaa aaaagttgat gattagttat ccagatgaca
     3901 tttttttgga gtccaatcgg agtgccccct ctttttttaa attatcagaa gtagaaaaag
     3961 aatatttcga atattggata gaaaagcaaa ctagaccggg tacgaaagaa gagtattccc
     4021 aaaaagttga tcggaattta attcactcaa ttcagattat tgcaagttta aaagatgagg
     4081 tgaaacaact ttgggagcta ctataacatc gaaaggacgc aagaaaaatt tatcctcgct
     4141 attacaatat gcagctaatc cttcaaaaaa agaaaaatgt gttgcagtct caggaataaa
     4201 ttgtagtgat gatagtgagg aagcactgca agaaatgaat ttagtacgta ccaagatgaa
     4261 taaatctaaa gatatagcac aagcgtttca tgttattcat tcgtttgata agagtacttc
     4321 taaagagtta agtattgata aaatgcacga aatagctgtc gaatttgcag agaaagcttt
     4381 tccaaattcg caaatcattg ttgcaagtca caatgataaa gatcattttc atagtcatct
     4441 tgtcattaat aatattaaca tggaaacatg taaaaggttg cgtattgacc ctaaagattt
     4501 agaactaatg cgaaaaatta acgatcaagt cctcaatgaa catggtcttg aatcagtgaa
     4561 taatgagtac tatgaaaact tagaaaagag aaccaatctt tattcaaacg atccttctaa
     4621 gatgaaatat ggaaaaatta attatcaaga gtttgtgcaa aatgcgatcg aagcaacgtt
     4681 ggaaaaatgc gaagtgaatt cttttgaaga tttttcggat catttggcaa aagaatataa
     4741 cattgaagtt tatcaatttt caaaaacaaa taacaaactt ggctatgttc tttacaaaca
     4801 aggtgtaaat ttctatgagc gtagaggtga gttaattact ttaggaagag ataaacaatc
     4861 tagaaaagaa aaaagtcagt atgtcgaacg tacttttagt gcaagaaagt tagggaaaag
     4921 atattcattt gaatctgttt ctgaacaact tgaaaagaat ttagaagaat cacatataaa
     4981 aaattttttt aatatggaaa gagagcaagt tatgtcgcta attagtggag taaattacaa
     5041 aactggtgag ttaaatgtac cgtttgtgga attagattcc atcgcacaaa agcaatttag
     5101 cgaccgtgaa ggcgatgaaa aattcattaa atatgatgac ttcttgcgag aggtagacaa
     5161 gcattttgta aatacaatgt ctttagaaga tgatcaagaa tacacgatta aacttcatac
     5221 ggatgtgaat cctcaaactt tacgtagtgc gaaaagtaaa acaatttcta ttaaaaagac
     5281 tgatcagaaa gagttatttc taccggcaaa gggttttgaa aattgttctc atacaccttc
     5341 tgctaaacag attcactatc aaatcagtcg cataatgaat gacgatgtaa aaatgtatcg
     5401 tgctaaaaag gattatgaat tcacacaaca aatgattgaa aatcagcaaa aaaggcaaca
     5461 aaattttgaa atggaattct gaatgggctt gttagtggac tttccagttt tttgcctatg
     5521 aagagttgct tggaaagaag cagtttttag gacatacgcc agctacccat ctacttgcca
     5581 gtcaatccgc cccgctcacg ctaggctact ttctggcaag tcagatggcg ggtaaatctc
     5641 tgagggagca agctccctac agactccctc ccaaaaaaag aaaggtggaa gaaaaaaatg
     5701 aactatccat attttaaagt ctcggcttca gaagaaacta aagaaatatt caacaatttt
     5761 tacaaccaaa acaaaggaat ttttggttca aaggcaaata tgtttcgtgt tatggtt
//
LOCUS       DAYYHX010000166         5787 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000166 DAYYHX010000000
VERSION     DAYYHX010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.167"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(268..1050)
                     /locus_tag="ACHA6Y_002311"
     CDS             complement(268..1050)
                     /locus_tag="ACHA6Y_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988386.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1115..1951)
                     /locus_tag="ACHA6Y_002312"
     CDS             complement(1115..1951)
                     /locus_tag="ACHA6Y_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8988387.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1969..3378)
                     /locus_tag="ACHA6Y_002313"
     CDS             complement(1969..3378)
                     /locus_tag="ACHA6Y_002313"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8988388.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3392..5215)
                     /locus_tag="ACHA6Y_002314"
     CDS             complement(3392..5215)
                     /locus_tag="ACHA6Y_002314"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8988389.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1778 a         1258 c          948 g         1803 t
ORIGIN      
        1 aaaaaaacag tactgtctag tagtataaaa tatacaaatt tcttaaaaat tttttcacta
       61 aaatcactac ctgaaacagt ctaaaacact tatatatcaa cgtttaatga aactatctcc
      121 gtttcatttc agatacaaat aaatatagtc gggctccaaa tccgaaaaaa aataccccca
      181 gtagcttatc ctaagctact ggagatgtat aagtacggtc atataacaac ggggtagggt
      241 aaataaatga aaaagatgga agaatcatta ttcaataact tttttaaata cttcgttgta
      301 atcagttacc tgtccgttct taaataccac agaataatct tttttcccat tttcaagtaa
      361 ccgaagtgga atgtctttac caacttgctc tgtcactgca ttactgatac ctgttagatt
      421 agctaataaa tctgataaag attgagcatc tgtagaaatc tctgtatcag tatttatcgt
      481 gattccttta tcttcattta caagcttcca agtaggtggt atagctgtgt atgtttcgat
      541 gaatgaatca attctttcat ctatcgtctt cccagtatct acttctgtct cattactctt
      601 agatgaatcg attctttcat ccatcgtctg ctccgtaact gcttctgtct cattattctc
      661 accggaaact ctcttcacac ccaaacttat atattgtcca tctttagtgt gcatagttaa
      721 aattcccacg cttttactaa taaattcact ataaccagtt ctttcaatag agacttcaat
      781 gtatttcaca ctatcatact caaaagaaaa atagacatgt aaaagcattt tgttgtttac
      841 atctacataa taatcctttg actctacttg agaccttcct atacggaata cagctttact
      901 gtctgatatc tctataccag catccttatt attaatctgc caatctccaa gtagctcttc
      961 tggcaagcta gtagtttctt gtgaacactt taccacttca ctggtagagg tcgaaacatt
     1021 cacctccgca ctgttagaag aattttccaa ggaatcagga gccttagcac aaccaacaag
     1081 tctttatata ttgtgcagcc gatattttct tcattcagta cacccttgaa atatgcagtg
     1141 ttagataaag cagttcgtcc tgatttgctg aggcacccat ctcttcttga acgtactcct
     1201 tgattttcaa ggccgcccga tactctttcg gaaaacgctg cttcactgaa gccagcaaaa
     1261 aatcatcctt ctccggcaac tcttccttct taatcattcg ggcaataaaa aactgtaaat
     1321 gcgtcacaaa tcttaagtaa agcatcgaat ctttatcaac ctttttttcg ggaaacagat
     1381 atcctagcat agccaagata tcctttagtg ctcttaccga gagcatggtt tcttccatat
     1441 ttgcttgttc actttgggca tttacaatgt gaaacgcaat gtttccagcc tcctcttctg
     1501 gcaactgaac attcattttt ttgttgatca attggatagc atagcagccg atctcaaatt
     1561 cttttgggta atacttttga atttcaatca gcaggcgatt ttgaaaaaca atgccttttt
     1621 gactgcgttc aatggcatgc ttcaagtgat caaccaaggt aaaaaataga cttttattca
     1681 gcttaaagtc aaacttggcg ttggcataat cgataattcg cttgacgcaa ataattagat
     1741 catccggagc agcctcaaac agtttaatgt aatccttttt tagagcatca gaaagtgaat
     1801 ctaatttaaa tactttttgg attttgtcag ccggaaccaa atcgcctttc tttagttgaa
     1861 atccaatccc tttccccatc aaaattactt ctttgccttg cttatctaag caaagaatta
     1921 aactgttgtt taacgtttta attactctca ttagattcac cgttctctct aagctaagcc
     1981 atttcttgcg attacttttt gataccaaaa gaagctttct ttttttattc gttttaattc
     2041 tttaagattc tcatcggttc ggtcaacata tataaaacca taacgcttat tgaagccttg
     2101 atgggtacta acaacatcaa tcgctgacca aggtaaataa ccaaaaacat ctacaccatc
     2161 tgttaaagct gcttgaatag ccagaatatg tttggctaga taatcaattc gatcctggtc
     2221 attaattcgt ctatcttcct caactatgtc gggtgcccca tagccatttt cagtaattaa
     2281 aataggtaaa tcatagcgat cgtacaattt cctcaatgtt aatcgtagtc cagtagggtc
     2341 aactacccag ccatatggcg ttttatctac ataaggattt tcagctgccc ggtagacacc
     2401 ttcttctccc aacatgatct gttgatcccc cgctctagcg gaaacatctg atccgtcatt
     2461 tttgctggca gcgatggtgg cagttgaata ataattaatc gcaataaaat cgggtttacc
     2521 ggaggataaa attgcctgat cctctgcctc aattttagga tataatccac gatcattcaa
     2581 atagttttca aacaaataat tgtattttcc ccgaactgct acatctaaga aagaccagcc
     2641 tctcaatgtt tcccaattat gggctgcaat tgcatcttct ggactggagg tagcttgata
     2701 catagccgtt aaattgattg ctggaccgat ttttccttta agattcaact gatgatacaa
     2761 gtgaattact ctagcttgag ctaataaaac atggtgatta atttgaaaga gttctttttt
     2821 tgaaggcagc tctccacttg gtggcaaacc aatcgctcca ggatgaagga tcatcgtgtt
     2881 ttgctcatta attgtcagcc aatagttcac cttgtcacca tactcagtaa gcaatacctg
     2941 tacatagtcc acaaaataat cgatagttgc tcgatttaac caaccgccct gctcctctag
     3001 aaaataaggt aaatcgaaat ggtacatggt gacaatcggt tcgattccct gattcaggca
     3061 ttcatcaatt acttgatgat aaaaagaaat tcccttttga ttggttttat tattttcaat
     3121 atccataatt cgcgtccaag cgatggaaaa gcgaaaagct tttaagccta attctttaaa
     3181 cagctgtata tcttctttat agtgatggta aaaatccgag gcaacttcaa aattagctac
     3241 cccttctgta tgctgatact tgtcaataat agacggttgt ttcccatcct cctgataggc
     3301 accctctgcc tggtaggcac taatggaggc accccataaa aacttctctg gaaatgctga
     3361 aagctgtttg tattgcacaa aaatcctctc cttaactaat ttgcatcaat ggttcgtgtt
     3421 cctttaagct tccgcttgct tcactaattc ctgcgtaatt ttcactgttg gtcactacca
     3481 caatcactgt atcatcgtag ccagcgtttc taatggcagc gaaatcggcg ttgattaata
     3541 attgtcctgc tgacacctga tctcctgctt ttacaaatac ttcaaagggt tcccctttta
     3601 aattcacggt gtcgatcccc acatggatca ataattcaat accttcttta gaacgtagac
     3661 caatcgcatg tttagattct aaaatagtaa tgacttctcc ctcaacaggt gaataaatgc
     3721 tgttttcttc tggatgaatg gctacgccct ttcccaatag tcccttagaa aagacagcat
     3781 cgttcacttc agaaatagga cacacggttc cttttaccgg attttcaaca atgactgctt
     3841 cggctattgc cgtttgctct gattcatcag aagtatcgtc aaatccaatt agataagcaa
     3901 tgataaaggt tgcaacaaag gcgattacac acgtaataat tgcgtgaaca atattcattg
     3961 gattttctcc aataaaggct ggtaaggctg ctaagccagg ggaaacaaag gcatatctga
     4021 ctaaaccgct caagccagca tacaaaccag caattgcacc accaatcatg gaagcaacta
     4081 atggtttttt cagcttcaag gttactccat agagtgaagg ctcagtaatt cccattaaag
     4141 cagtgatccc agtggacaca gctagctgct tcaactcttt gtttttagtt ttcgcggcta
     4201 cagcaaaggt agcacctgat tgagcaatat tagaagccag cattcctggc ccattgatca
     4261 tttcagaacc cgtactgctc aattgcatag tagcgattgg cgtcattgcc caagctgtac
     4321 ccgtaaccac caaaaatggc tgcaaaccac ccatcaaaaa tggaattaac caagctgctt
     4381 tgccgttaat ccattcagcc cccattgcta taagatcgtt cagcaacgtt ccaaacggtc
     4441 caatgacaac caacgctaaa ggccccataa ttaaaaatac cagcatcggc ttagtaaaga
     4501 atttaataaa cgaaggcgaa attcgatccg caaagggctc tacataggac atgatccaaa
     4561 cagtaaagat aatcggtaaa actgatcccg cataatctgc agctaatacc ggaattccta
     4621 gaaaagaaac ggcatcgcca gtagaaaaaa gctgtgaaat atttggatga agcaaaatgc
     4681 cagcaacagt aatcgccaaa attgggttga catcaaactt tatggctgcc ccataggcta
     4741 atagtactgg cataaaataa aaggctgcat cagcaatagt attcaaaatg atgtaagttt
     4801 gactactgtc tgatagtaca ccagtcaaag tcaagacggc caaaatggct tttaccatcc
     4861 cggcacctgt tatagctgga atcatcggtg taaaggtggt agaaatgaca ctaatcaatc
     4921 ttgaaataat actttcttga ttgtctgaag aggggccctt tttaattgaa gttcccactt
     4981 ccttttgaat ttccttgaag gctgtttgga cttcattgcc aatcaccact tggtattgtc
     5041 caccgctatc caccacacta attacaccaa gtgttttttc taaggctttc gtatcggctt
     5101 tccttttatc tttgagttca aaacgcagtc tggtggcaca atgggttaag ctgaccacat
     5161 tttcttttcc cccaacctgt tgcaatacat ttcttcctac ttctctgtaa tccattattc
     5221 tctctcctta aagcagtaaa aaagaccaag tcaaaccacg ccccaatcaa cattgaagtg
     5281 ttagtttgtc ttggtcttgc ctgcataatc agtaacatgc caataacgtt attttgctgt
     5341 aatccaataa tagcacatta tgatagcgca atcaacaggg aatttaattt tttatctttt
     5401 cgtttgaccg tatagggtaa tataacagtt agatcacatc aactagggta tatctgcgta
     5461 tacttactgc taaaataagt atatctaata ttaactatac cagtaaaacc attcctacat
     5521 aaccagcggg aaattcccaa aatcgtgaaa gcatcaaaaa tgcatataac ggatcacaca
     5581 acttcaacaa ttctgctctt gggaaaaatt gatgaatttg gtgagtaaag gatggaataa
     5641 acagcattcc aacaatatag gcaacaatag tgatacttgt ttttttggta aaaatcagta
     5701 agcaatagcc aatttcaatc attccgagat ataaaggtat cgatagaatg atttgggcta
     5761 gtattcttaa aaattaacta gcaaatg
//
LOCUS       DAYYHX010000167         5678 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000167 DAYYHX010000000
VERSION     DAYYHX010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.168"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(240..606)
                     /gene="ssrA"
                     /locus_tag="ACHA6Y_002315"
     tmRNA           complement(240..606)
                     /gene="ssrA"
                     /locus_tag="ACHA6Y_002315"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(777..1550)
                     /locus_tag="ACHA6Y_002316"
     CDS             complement(777..1550)
                     /locus_tag="ACHA6Y_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8988390.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1694..2044
                     /locus_tag="ACHA6Y_002317"
     CDS             1694..2044
                     /locus_tag="ACHA6Y_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8988391.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2025..2741
                     /locus_tag="ACHA6Y_002318"
     CDS             2025..2741
                     /locus_tag="ACHA6Y_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM8988392.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2741..4189
                     /locus_tag="ACHA6Y_002319"
     CDS             2741..4189
                     /locus_tag="ACHA6Y_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8988393.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4260..4769)
                     /locus_tag="ACHA6Y_002320"
     CDS             complement(4260..4769)
                     /locus_tag="ACHA6Y_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988394.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4759..5483)
                     /locus_tag="ACHA6Y_002321"
                     /pseudo
     CDS             complement(4759..5483)
                     /locus_tag="ACHA6Y_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1935 a          984 c         1120 g         1639 t
ORIGIN      
        1 gtttttcctt ccatcaacat aatgttcacc tcaaaaagag tatacgaact aatgttcttt
       61 ttgtaaagta ggaatatcat tgataatatg gatggaacat atatattaaa aagtgttctt
      121 tttgaatgct attttgaatg ccattttcat tcatttaacg aaaatctgcg aaaataaaaa
      181 atgctcaaac ccttataaaa taaggattta agcatctagc gaaatctgct gaaatctcac
      241 aatggagacg gcgggagtcg aacccgcgtc caaacacatc gccacttaaa aatctacgtt
      301 catagtcaac tcatttaagg attcacttta cagcttgccg agtgacaggc attctgtatc
      361 gctagtctga tgttctcttt ttcaacttac agacggaaag ttgaaacgta tcccactaaa
      421 gttaggaccc ttactcgagc acatgggcga tgccgagagg atctacgcta actgttttta
      481 ggcagctaaa gcgtaagagt tgttttcgtt tttagcagtt atatttaact gtaacgtttt
      541 tacgtagacg taacctacga aacgcaattc aagctcgact gtgcctgtcg aatccgtaac
      601 gtccccgtta gctaacaagg gttacgtgta tttttcatac aaaattagta tagcatatat
      661 tgggataaat tcaataaaaa agcgaatcat atcccattat ttatataata actgcccctt
      721 tcttaagaaa aaataagcga ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa
      781 atcagatcaa aatacttcaa tgcgtggaca attccgcctt ctgtattttt ctttgtgata
      841 aaggtagaaa gttcttttaa tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa
      901 gcttccaaca aggcaaaatc attcggacca tcgccaaatc caaaagtagg aactttttcc
      961 agctgaagtt ctttttgcag acgtttgaca cctgcccctt ttgagattcc tttcttcact
     1021 gtatcgatcg agtaaggacc attccgataa aaagttagct caggaaaatg ttcgtgatag
     1081 tattcgtcac ctgattgaga taagatcagc aacatattta ctgtgttttc tttaaagcct
     1141 aaaggatcaa tttccggaac atctgaatga atgaagtcgt aagcattgat cattgtttgg
     1201 ctatgaccgg tacaccagat tttctgatcg ttatagtaag aaagttcatg accgttcaac
     1261 tgtacatgat catacatttt ttgacattct tctgaagaaa atgtttgaga atagatttct
     1321 tcgccgtcta ctcgaatgaa agaaccattc atggtgaccg cagaagtaat gcccgcatct
     1381 tccatgatag agtggatttc tgcttctgta cgtcctgtag cgatgacggg gagaacattg
     1441 ttttctttta atgcgttcat agcttttgcc acttcgggtg tgatttttga tttcgcatct
     1501 agtaatgttc catcaagatc aaagaaagtg attgcacgat aatttgacac aaataaaact
     1561 ccttttttat tggaataagt ttatcttaac aaagaatgaa caaaagacaa acctttcgtg
     1621 aagaattcct gaacatattg gttcagtcaa caatttagag tatactataa gaagagaatt
     1681 taggagggaa agaatgattt ttttcagtat tggtatctta ttcttaataa ttggcagtct
     1741 ttttcggatt ctgccctcaa aaggaaatct gcctttttat gggtatcatt cgcctcttgc
     1801 cgcaaaaaca gacgctcatt ggcggctggc tcaaaagaca agcggaaact ggttttttct
     1861 tatgggctta ctgatggcat tgatcggcta ttatttaaag acatctgggc atacgaatta
     1921 ttttctgatt gaaatgcttc tgctagtatt tccaattatg cctattttta ttatgacaga
     1981 aaagaaatta cagaaatatg acctggaaac tggagggaac gataatgaat attttaatga
     2041 ttgaagataa tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat gaaggttggg
     2101 aagcaacttt caagtatgat ggaaaagaag ggctcgatgc ctttttagaa aacccacaac
     2161 attgggatat gattacacta gacctgaatt tgcccacact ggacggtatg ctagtaggaa
     2221 gagagatccg caaagtttca agtactgtgc cgattatcat gctgacagct agagattctg
     2281 aaagcgatca ggtgatcggc ttagagatgg gggcagatga ttatgtcaca aaacctttca
     2341 gtcctctgac gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag
     2401 caagagaaga agcagtagtt tcagatgatc attttgatat cgagactagt catttcaaga
     2461 tgaatacgaa aacacgagaa gcgtatctgg acaaccggcc aattgaagga ctgacgccta
     2521 aagagttcga tctattgttt actttagcaa aaaaaccacg tcaagtattc tctcgggagc
     2581 agttgcttga attagtctgg gattatcagt actttggtga tgaacggaca gtggacgccc
     2641 atatcaagaa attacgacaa aaaatcgaaa aggtcggacc acaagtcatc caaactgttt
     2701 ggggagtcgg ctacaaattc gatgattctg gtgttgccta atgcgttatt tgtatcagca
     2761 actcttagct ttctgcggaa tggtcgcgtt gatcttattg atcgttggag tttcttttac
     2821 acaactgaca aaacaaacga ttgaagaaaa caattaccag caactgtttg gatatgctga
     2881 atcagttgaa aagacaacgc aaacctatgc cgatacgctc ccgcagttta gtcaagatca
     2941 ggcgtttcaa aatgcattgc tgctgacaga gcaagtactg acagaacaaa atgtgaactt
     3001 tatatttatc gataaaaacg aacgtgtcat ttatccgact aatacagctt tactgaattt
     3061 taccttaaca gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc
     3121 gaataaaaat attttaggac aagcacaggc gacctcttac gcacttgtgc catttaattt
     3181 gaatcatgaa ttctatggag cacttgtagt tagtcaacct gcgcgaaata tcgataacag
     3241 cgttcgtcca attacactga acctgttcaa aggatttatt ttttccagta tcgttgcagt
     3301 tattgccagc tatgcttttg cagcattcca agttaagcgt atcaatcggt taaggaatgc
     3361 gacgaaagaa gtgacgaatg gtaattttga tgtacagctc cccgtacatg acaaagatga
     3421 atttgatgag ttagcagatg atttcaataa aatgacgaat tcattgaaag aatctcaagc
     3481 agaaattgaa gaacaagaaa atcgtcgaag acaatttatg gcagatgctt ctcacgagat
     3541 gagaacacct cttacgacaa tcaacggact tctggaaggg ttagaataca atgcaatccc
     3601 tgaaaatcag cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc gtctgatccg
     3661 tttggtgaat gaaaacttgg attatgaaaa aatccgtacg aatcaaatca gtatggtcat
     3721 taaaaaattc aatggaacag aaacattgaa aaatattgtt gcacagttag aagctaaagc
     3781 agaagcagca ggagatacat tgacattgaa agccgttgat gatattgatg tttacgctga
     3841 ttatgaccga tttgtccaaa tcatggtcaa tattattcaa aacgcgatac agtttactga
     3901 aaacggccaa atcacgatta ctttagaaaa aggctatctt gaaacgatca tcacgattga
     3961 agatacggga attggaatgt ctgaacaaca aatgaaaagt atctgggatc gttattataa
     4021 agtagatcct tcaagaaaaa atacaaaata tggtgaatca ggattaggtt taccaatcgt
     4081 gcagcaactt gtacgcttgc ataaaggaaa attagaagta gaaagcgaac taggaaaagg
     4141 aacaaaattc gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg
     4201 tataaaagat catgaatcga tcttctatac cgcttgtttt ttactataaa atgattgatt
     4261 tagcccgcgg gctctaaacg atcaatataa ggactttgag caatagtagc atcatgagta
     4321 aagagtaaga taccttgatt ttcttcatga gctaaataag aaagcaagtg gagtagctct
     4381 tggatctcca atacactaag ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg
     4441 gaaagcaagg catgaagtaa ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc
     4501 gcacaattaa gcaaggaagg agagatatca agtttttctt gataggccaa acgatcttgt
     4561 ttgatttctt tttccggtac atccaacagg acatggtctt taagattcag ataaggaaac
     4621 atcggccaat ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt
     4681 ttcaagtgct ccaatatttt atgctgtttt tcgagaggac acaaaataac ggtcaagtgt
     4741 tcagcatgaa tcaaatattc aggtttcatt acgtccaggc ctcctacttt gcaaatgatg
     4801 gataaaaaag tgaaagggaa tagactaaga atgagacagc atccactgag cccaataaga
     4861 gtttgggcaa atcccttaag tatatctgtg aaaaaaagat cattttggaa atcgatggta
     4921 aaaaaagtct gttgatccat cggaattgta agtccgctta ccgaagtcaa gatatcacgg
     4981 atatctggca gttcagtagt ctttaagaca agaaaattga tcctttgaag aagcgcttca
     5041 taaactgact ggaagcttac gatccacaat aaaaccaaga tacaacaaag aaagagagga
     5101 aagaacagct gccagaaaat aaaaaggaat aatttggttt tagaaagacc agtcaggtgc
     5161 caattgatca attcctgctt gttttgcctg actgaatagt aaaagaaaaa gataaaaagg
     5221 aaggcaaata gaaaaaataa actgaatatg ccttttatat aaaatcggct agctaattct
     5281 aaagccggaa gaagctctgt agatgcagaa gcgccaataa atgaaagccg atcttggact
     5341 tgattggcta aagccgattg caaatggtaa agagtcaggc atgctatcaa taaaaaagca
     5401 gaaaagcttg aaaataaaca atagagtatt gtctgctttt tgtaataaat taatcgagca
     5461 gtagcatagc ggattatttt catacttttc acctctataa aaagtataga aaatgaatat
     5521 gaataaagta tgaaattcct ttagtgaaat cacgaattaa aaccagaaaa tattttgtaa
     5581 aaagcaagag gttgtgaaga agctgcccta ttctattacc gtagatgcaa gcttttgaac
     5641 actgtttatt cgaaaagagg agcagcgaca ataatttt
//
LOCUS       DAYYHX010000168         5670 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000168 DAYYHX010000000
VERSION     DAYYHX010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.169"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..768)
                     /locus_tag="ACHA6Y_002322"
     CDS             complement(16..768)
                     /locus_tag="ACHA6Y_002322"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8988395.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1132..3798)
                     /locus_tag="ACHA6Y_002323"
     CDS             complement(1132..3798)
                     /locus_tag="ACHA6Y_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8988396.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4139..5590
                     /gene="cls"
                     /locus_tag="ACHA6Y_002324"
     CDS             4139..5590
                     /gene="cls"
                     /locus_tag="ACHA6Y_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8988397.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         1642 a         1193 c          991 g         1844 t
ORIGIN      
        1 cataaaaaga acccttcacg agtcgtgaaa ggttcttttt ttatctgcgg accaaaggag
       61 cagtctatag gtcagcttag tcgtactaag gatttctcgt ttttcactga atagtttgcg
      121 aatgtacctg gtcacaacag gatcttttgc gtacgcgtca tagtatagct tcatcaattc
      181 ttcagtaatt gattcctgtg taaaataagt aaaatctttt ttcttcactt tgaatccttc
      241 atcaattaag tatgtttcaa attgatccat tacaactggc gatgtattag gatcttcttc
      301 tatcattcct agttgctttt cgataggata aaaaacatca tcgattgatt ccactactct
      361 gattatcacg caccatttgc ttgacttttt catcaatttt tctaattgaa taagatgtac
      421 caatgctgga tttgctgctg taaaaaccat ttcatatgtc tcatttgatt ggataaattc
      481 ttctaacgac tgtttgataa aggaaacatt tggcaactca tttttttctg cttctttttt
      541 tgcattttct agcattcctg aagaaaaatc gatcaaatca tagcgattaa tttcttttgc
      601 taaggaaaga aatctgccag atcctccagc aacgtctgct gctgtttcag caggcaggac
      661 tttctccgtt ttcagataat tttttacatc ttcagcaata gttacttgcg attcttgctg
      721 gatttcataa tattcttcag caaattcatc ccattctttt tcattcattt gatttcctct
      781 aattttttgt tatatgtttt cacttttcgg aataaatcat tctcctcttt tatctattct
      841 atctcttttg atgaataata aaatgattgg ctttccgtct atcataaata aaaaaaggga
      901 ctgtgaccaa agtcgtgtca aagtccctct ttccgaataa acagtgtcca atcagatgac
      961 cctcagtatt agctcaaaat gctgatttta caacttcttc cttaaacggt gttctcctgc
     1021 caactcttcg gtcacaagtc acaatattca acaaacatga gaagctgttt gttgaaattg
     1081 caccttgtga ctcagaatta aacggcaggc tcacaacctc gttgattcgt tttatttatt
     1141 tccttgtact cgtacaatga tcaatttagt gatttccatc aaaacaattg tcacaagtgc
     1201 gattcccata catgtaaaga cttctgtcaa tccaaatgct gatgggatag agaagacttc
     1261 tttcagtcct gggatcattg ttaagcagta aagcgcgcta catacaacta cagctagtaa
     1321 gacacttctg ttagagaaga accctgcttg gatagctgtt tgtgtgtttg aacgtgctgg
     1381 gaatgtttgc aatgtccgac accaaatcaa tgttgaaaat gacatggcta caccgaattc
     1441 aggtgaatgc tgcatgccaa gccattgagc aaagataaca gcaatcccaa tcagcacacc
     1501 acgatacgtt acagaaatca atgttttgtc cgtaaagatt cctgaatttg gatctcttgg
     1561 cgggcggttc atcgtagcag gttcagcttt ttctgtacct aatgcaattg ctggcagtga
     1621 atcattgact aaattgatga acaacagttg taatgctgta aatggattgg accagtcagc
     1681 aactaaggca aagatgatcg caatgatcgc acctaaattt cctgcaaata gataagaaat
     1741 agctttcttg atattatcaa acacattacg tcctacttct actgcgctga caatagaagc
     1801 aaagttatca tccgtcaata ccatcgctgc tgcatctttt gccacatctg tacccgttcc
     1861 catagcaatt ccgatgtctg cttgtttcaa tgctggtgcg tcatttacac catcacctgt
     1921 catagctgag atatgacctt tattttgcca agcacgaacg atacgaattt tattctcagg
     1981 tgaaacacgt gcataaacag tgactttttc taaaacttcg tttagttgat cttctgtcaa
     2041 ttcatctagt tccgtcccgg tcaaagccag gtttccttct tcaaaaatcc cgatttgttt
     2101 tgcgatagca acagctgttg ttttgtggtc accagtaatc atgattgttt tgattcctgc
     2161 acttcttgct tctgttaccg cctgagtcac ttcagcacgt ggaggatcga tcatagcaag
     2221 caaaccaaca aggatcaaat cgtcttcatc ttcaaatgat aaatcaaaat tatcaagtgg
     2281 acggtaagca aaagctagta cacgtaatgc gcgtgttgaa aaagcttcat tttgctcttg
     2341 gaattttttc atcaattcat ccgtcatcgg aacgacttca ccatttagca atactttttt
     2401 actgcgttgg aagacgatat ctggtccgcc tttaacaaac aagtaatttt tgttatcaat
     2461 tgtgtgtcca gtagacatta attttcgatc tgagtcaaaa ggaagttctg cttgtcttgg
     2521 gtagttgttt cggatttcct gataaggctg gtttaatttt tcactaaagt cgataaacgc
     2581 tacttccgta gggtcgccca atttagtacc gtcttcactg atcgatgcat cattcgctaa
     2641 aacactgatt tcgatcaagc gacgttcgtc agctgtccat tttgaagggt catttgtgaa
     2701 atccccagta tcgccattac ctaaaaagta gtcaacaact gtcattttgt tttgtgtcaa
     2761 tgtaccagtc ttatctgtac agatgatact tgtcgaacca agtgtttcaa ctgctggaag
     2821 cttccgaata atggcatgac gttttgccat tttattcgtt cccaaagata acacgattgt
     2881 tacgatagac tgtaatgctt ctggaatagc agctacggct acggctacgg caaacatcac
     2941 tgcgctgacg atatctgcag tcatatctcc tgtaccttct ccaagataaa tacgaaacag
     3001 ttggatacct aaaatcaata aagacaatgc caaaatagcc aaacttagtt ttttactgaa
     3061 tttatccaga cctcgttgta acggagtgac ttgctcagtt gttgattcca aaaggccagc
     3121 aacttgtccg atctccgtat tattcccagt tgaagtcact aagaatttac cgcgtccgta
     3181 agttacgatc gttccactga ataccatatt tgtacgatct ccgattggta ccgtacttgt
     3241 gatatccgtt acttcttttt ctgcagaagt cgactctcct gtcagcatac cttcatctac
     3301 ttttaatgat cccgcatcta gtaaacgtcc atcagcaggt acataatcgc ctgcgtctaa
     3361 caacacgata tctcctacca caagttcgtt tgccggaatc gttttttcct taccatcacg
     3421 aatcactcga gcatcgggcg ctgaaaggtt tttcaacgct tctaaagaac cttctgcttt
     3481 ttttgtttgg acaacactga ttatagaatt caacatcaat actgcaaaaa taactaagaa
     3541 ttcgatgtag tcacccatga ccatctgaat cagtgcgaca atcagcaata cgataaccat
     3601 tgcatctttg aaagtttcta agaacaattt ccaagttggc ttcttcggct tttcttttag
     3661 ctggttcaag ccatctcttt ctaatttttc cttcgcttgc gtggtgctca atccatcggt
     3721 tgagctctgc gtttgctgca tcaacgagtc gatcgtttct ttatagaccg cttgttgtgc
     3781 ttgttccttg ttttccaaat ttcatccccc attttttgat cttggcccta gtcctgtctg
     3841 aaaaaaaaga gacctaagac aaatacgcat ttgtcttagg tctcaccatt tcatacaata
     3901 ccagaaaacc atttggttaa cttactgttg atattatcgc aacaaacgtt gccggttact
     3961 cccctaagga gatattcaac tgtactaata tcctacctta ttaacctaca aaaagcaact
     4021 actttagccc gatttaccta cattttcatg aaaggagtga aaagtatatt caccttggta
     4081 ggcttctttc ttctatcatg ctatactaat gttattgtat gtaaggagga acatttttgt
     4141 ggtatctagt attataaccg ccctttatct attaaatgca cttattgctt tggtggctat
     4201 tttgatcaaa ccccgagatg tagcagccat ttgggcatgg ctcttagtat tgtttgccct
     4261 tcctggggtg ggctttgtat tatatttatt tttcggacgt ggattaacgg ataagaaaaa
     4321 attctatctc cgacaaagtg acttgaaaga attagaaaac tttcagtctt ttagaggaga
     4381 taccattgaa cattacgatc ctgacatggg cgataaagac aaacagcaat ttgttgactt
     4441 cttctcttca ttaaatcgta tgccgctgac aagaatgaat tctgtcactc ttctcacaga
     4501 cggacaagag aaattggatt cactgcttca agatctaaaa aaagccaaac attcgatcca
     4561 tatcgaatat tacgcatttg tgacagataa tatcggccag caagtcttac atgttttaga
     4621 agaaaaagcc gcagaaggcg tggaagttcg aatattatat gatgcatttg gctctcatgg
     4681 cacaaaagca aaagatttca atcgtctaat caaaaatggt ggacatgtcc atacatttgt
     4741 tacctcacaa agggcattac ttcgtttccg attgaattac catgatcacc gaaaaatcgt
     4801 tgtgatcgat ggaaagatta gttataccgg tggtttcaat attgccaatc aatatgtaaa
     4861 tacaacaaaa aaattcggct attggcgcga tacgcatata cggattttcg gtgccgcttc
     4921 ttctttgctc cagcttcgct tcttaacaga ctggaacgtc tcggtacctg aagaaaaaaa
     4981 ggtcggctat catttgaatt atttctttaa aaaagcagat cgagatgaat ctaagcttgc
     5041 tgatacatcc atccagcttg tttcaagcgg accgaataac gaaagggaac aaatcaagct
     5101 ttcatttatc aaattgatta cttctgctaa aaaacgtgtt tggatacaga caccttacct
     5161 tgttcctgat gaaagtgtca ttgctgcttt aaaaatcgca actgcctctg gtgtagatgt
     5221 gaaaatcatg attcccaaca aaccggatca tccttttatt tatcgagcaa cacaatatta
     5281 tgctcggcag ctgatcaagg aaaatgtaca aatccttgtc tatgagaacg gcttcctcca
     5341 tgcaaaaaca ttgataatgg atgatgaaat ctgcatggta ggttcagcaa atcaagatat
     5401 tcgtagctac cgattgaatt ttgaaacaag tgctgtcatt tacgatcctg agtttttaga
     5461 agaacttgct actcagttca aagaagatga gacacattgt tcatccatga caactgaaac
     5521 agtcaaggaa atgtctaact ggctattatt caagcaacaa atttctcgat tattttctcc
     5581 aatcctataa tttttttatt caagtagaga aaaatatact aagattaaag ggactgtgac
     5641 aaaaatcttg tcacagtccc ttcttccgaa
//
LOCUS       DAYYHX010000169         5645 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000169 DAYYHX010000000
VERSION     DAYYHX010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.170"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..542
                     /locus_tag="ACHA6Y_002325"
     CDS             108..542
                     /locus_tag="ACHA6Y_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8988398.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(620..1153)
                     /locus_tag="ACHA6Y_002326"
     CDS             complement(620..1153)
                     /locus_tag="ACHA6Y_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM8988399.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1247..2425)
                     /gene="mutY"
                     /locus_tag="ACHA6Y_002327"
     CDS             complement(1247..2425)
                     /gene="mutY"
                     /locus_tag="ACHA6Y_002327"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM8988400.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2418..3215)
                     /gene="recX"
                     /locus_tag="ACHA6Y_002328"
     CDS             complement(2418..3215)
                     /gene="recX"
                     /locus_tag="ACHA6Y_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM8988401.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3322..4704
                     /gene="rlmD"
                     /locus_tag="ACHA6Y_002329"
     CDS             3322..4704
                     /gene="rlmD"
                     /locus_tag="ACHA6Y_002329"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8988402.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4878..5456)
                     /locus_tag="ACHA6Y_002330"
     CDS             complement(4878..5456)
                     /locus_tag="ACHA6Y_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM8988403.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         1669 a         1124 c         1015 g         1837 t
ORIGIN      
        1 tatattcgtt aaaaattctt ttattctttt tttatttttg aaaacttgcc atacttttta
       61 tttgttttat tttccagtat actatacgaa aggaaggtgg ctagaccatg aaagttgtac
      121 aaacaaaaga tacaatgagc aatatttact tagatgctgt acggatcagg catcaagttt
      181 ttgtagtcga acagggtgtg cctttgtctc gagaaataga caaagatgaa gcccattgta
      241 tccacttcgt cctgtactca gataaaaaag aaccgcaagg aactgtacgt cttttgcctt
      301 tagaaaacgg aaaaatgaaa ttacagcgga tggcgattct ttctgaatac agacaccaag
      361 ggttaggaaa aatattgatt gaagaagcag aaaactttgc taagaatcaa ggctataata
      421 cgattttatt aggggcacag tccacagctg aaacttttta cgaaaaacta ggatatacag
      481 cttacggcga tccttttgaa gatgctggca tgcctcatat ttatatgaaa aagactttat
      541 aaaaatttat ttcgaaaaaa gcaaaaaaca gcaagctttt gagaaaaatc tctcactggc
      601 ttactgttct tttttgtgtt tacttacgcg acaattgctt gtaacggtta taccaaatat
      661 caatatagcc ttccgagaac gggccatctc cattattgat ccagtcaacc agaatcttca
      721 cattttcttt taggatgaag tcaatatcgt tcgggtaatt catcattttg ctgtgcatct
      781 catattcatc gacatccaat agtcgtttct cgccgtcagg aaataccttg atatccaagt
      841 catagtcgat atatttcaag gcctcatcgt ccagcaaaaa aggtgaagct agattacaat
      901 aataggaaac ccccttttct ctgatcattg ctattacatt gaaccagtat tttttatgaa
      961 aataaacaat cgctggttcg cgcgttaccc atcggcgtcc atcagattca gtgactaatg
     1021 tgtgatcatt cacgccgatc atagagtgtt cgcttgtttt taataccatg gtatctcgcc
     1081 acgttcgatg caatttgccg tcgtgcttgt aactttggat cgtcacaaac tctccttctt
     1141 ttggaactcc catagcaacc cttccttaag cagcattttg tgctttgtgt ttgggtgttc
     1201 attacaccga catcataatt attatatcaa taaaaatgag ctttgcctag aaaggattcc
     1261 tattgtttcg atttttctct aactgatcta ccagtttcat ttggacctta ggaaaaacga
     1321 ccgaatcaaa tgcatcaggc ttcaaccact tcgaagtttt gttctcggta aattcttgtt
     1381 ctgctccctc ggttgcccgt ccgtaaaaaa ggagaacatg ccatttcaga tgactaaaaa
     1441 tatgagtaac ttctcctaaa tgtcgtgtct gccaaacaaa gagttcctgc ttttcaaatg
     1501 ggagattttg ctcttcttct gctactaggt catcaaacag atcgatttct ggctttgttt
     1561 cccattcttt tttcaatcgc tcatactcct cttgtgtcac ttccatcatt ggaaatgtcc
     1621 acatattcgc cagcaatttt tggctatctc tttcttcaaa ataatacgct ccagaatgat
     1681 tttgaagagc tgcactgata taataaacgt cttttggctt tgcttttttt gtttttacag
     1741 gaaaactcgt ttggattcct cgtttgttag ctaaacaaaa ggcttggatc ggacaagtct
     1801 cacactttgg agaagttggt gtacagatag ctgaacctag atccatcatt gcttggttga
     1861 attcacctgg atgcttttca tcaatgattt tccgcatcgc ttcatcaaag attttcctgc
     1921 tagaagcttt tgcgatgtct gcttcaatgc aaaacaatct gctgactact cgcatcacgt
     1981 tgccgtcaac tgcaggttct ggaagtccaa atgcgatact tgcaatcgcg cctgtcgtat
     2041 atggtcctat tcccttcaat gagctaattt cttcaggcgt ttgcggcatt tccccatcaa
     2101 attctgacat gatctgttta gcagcagctt ggatgttccg ggctcttgag taataaccaa
     2161 ggccctccca tgcttttaaa agcttttctt ctggagcatt tgctagctct tcaattgtag
     2221 gaaaccattc catgaaacga taaaaataat cgatcactgt gtctactctg gtttgctgaa
     2281 gcatgatctc agagatccag atacgataag gatcacgatt atagcgccaa ggcaggtttc
     2341 ttttttcttg ttcataccat tgaatgaact gatcttgaaa ttcttttgtt tctttatctg
     2401 tccatttttc ccattcttta ttcactttcc tcttcctttt ccgtaatgaa tcgctggatc
     2461 atatccaaat caaatccttt ttgatataag ctttgcttta cttttagatt tcttttttta
     2521 ggttcaaacc ttaaatttct tcgccatagt ttatctcctt gtttgactaa ataatcatat
     2581 tctatttctt gatctgcttc ttgcggaaga tcagctatcg cttgttggat cacttcttga
     2641 gaaaagcctt tcgtcatcaa tgtttgttgg atttttctca ataattcttt ttggcttttt
     2701 ccgtgatttt ttccaaacgc tttttcggct gtctgatgtg cattttgaac ttggtcttca
     2761 aatgtatatt ggctgagcgc ttgttcaatg atttcaggtt tcaaaccttt ttgctgcatc
     2821 tgctgcgcta atttctttgg tcctttgtca cttaagcgca tattcgtgcg cacataactt
     2881 tccccataga ccaaatcatc gacaaggtcc aattctttta agcgggctac gattttattt
     2941 cgatcttctt gaaggatttc cttttctttt aagtatgttc ggatttcttt ttccgatcgc
     3001 aactgataac ttaagtaatt cattgctagg tgaaaaccga aatcagctcc gctactcttc
     3061 ttgatctctt ggatagtttc ttcgtctaat tcccgtcctt ttaataatcg atagcgtact
     3121 aatacatctt cagatacttg taaagcttct ccttcagata aatctacgcg atagaaaggg
     3181 cctttgcctt tactgattct cgtaacggtt aacataaaca tcctcctcga acatatattc
     3241 tctatcatac cacagaaaat ccccgtaatc aggtattata tgttagaata aaccacagaa
     3301 aaaagaaaaa gggagatact tatggcagaa acaattgtca aaactggaca aacggttcct
     3361 ttgaaaatca agcgtttagg cataaatggt gaaggaatcg gttatttcaa acggttgatc
     3421 atatttgttc catatgcact accaaaagaa gaagtactag tgaaaatcac gaaagcaact
     3481 cctcgctatg cagaaggaga attaataaaa gtcaaaaaaa caagcaaaga tcgcgtagtg
     3541 gcaccatgtc ctgtttacta tgaatgcggt ggctgtcaat tacaacactt agcttatcaa
     3601 gctcaattgg attttaaaaa ggatctgctt ttgcaagcat tggaaaaatt caaaccagca
     3661 ggctttcgat cttatcaact tttgccgacg atcgggatgg atgagccttg gcattataga
     3721 aacaaagcac aatttcagtt acgaaaaaat aagcagactc aaaaaattga agctggttta
     3781 tacgaagcaa actctcatca gttagtccct ataacagact gcttggttca agaacctcga
     3841 acacaaaaag taatgaatac tgttgttcaa ctattgaata aatttgatgc acccatctat
     3901 gatgaacgga caaacagtgg aatcttccgc acaatcatgg ttagaatcgg tgttaaaact
     3961 ggggagttac aagttgtttt tattactcgc agtaaaaaat tcccgcaaaa aaacgcaatg
     4021 atccgtgaga tcaatgaacg actgcccgaa gttgtttcaa tcatgcaaaa tgtccaggca
     4081 caaaaaacgt ctctcgtgat gggggatgaa acgatccact tatggggaaa agaagccatt
     4141 gaagaacaaa tcaatgaagt gactttcgat ctttctcctc gtgccttctt tcaactcaat
     4201 cctcagcaga ccgaattgct ttatggcgaa ggaatcaaag cattagatgt ccagcctaat
     4261 gaaacgatcg tggatgctta ttgcggagtt gggacgatcg gcttaagtgt agcaaaacaa
     4321 gccaaagaag tacggggaat ggataccgtt cctcaagcaa tcgctgatgc aaaaaaaaat
     4381 gcggaaagat taggatatca caacactcga tatgaagtcg ggacagcaga gcaagttctg
     4441 cctaaatggt tgaatgaagg ttttcagcct gatggtatca tcgtagatcc accacgtacg
     4501 ggtttggata acgcattgat ccaatcatta ttgaaatacc ctccaaaaaa acttgtttat
     4561 atctcctgta atgtttctac acttgccaga gatctggtta atttaagtaa aaagttcaag
     4621 attgaatatt tacagtccgt cgacatgttt cctcagactg cacgttgtga agttgtcgtg
     4681 aagatgacga gactcgctga ctaaatgaaa gaggttgtga aaaagtctgc cctgtatcaa
     4741 gtaataagaa cagccaaaca aaaatagctc ttcatgtttt atgaatactg tacttgtgac
     4801 cgaagagtta acaggaggac actatttatt taaaaaaagg gttgtgacat agtttttgtc
     4861 acagcccttt ttgattatta ttggatattc acgtctatca cttgataaaa tgcatttgtc
     4921 gtatcgctga tattccatac tgctaaaatc acattatatc cttttcgatc ctgcggaagt
     4981 gtgatcgttt ggttgactgt ttttggtgga acagttgctt tgtcgtctat ttttgtgatc
     5041 aattcaaaat cactaaattt caaaggttga ttaggattcc aagttggttt ggtaatgtag
     5101 taatcccaag tacttgtccg atgttgtgct gttaaattcc aagtaatatt taatgttcct
     5161 ggattaacca tgtttttatg ccatctgtta gatgtctgtt catctaaagg ctcaaagccg
     5221 ctgactcctg cactagcaat ttttccatca atgaatgtat ttttggtagc ttcgatactt
     5281 tgcggttccc attgtgctct tccgacattt tgatttaagt ttcctgctgc tgatgttcct
     5341 aggtatgcac ggcttcctgg agaagtaata taaccatgcg caaaacctgg aatgctaaaa
     5401 ccgaacaacc ctaaactaac taacccaata aacaataacg tactaatctt tttcatgtag
     5461 aaacctcctt catttgataa ccacattgta aacgctttat tgtattaaca caaacattat
     5521 gcttttataa ttttatgttg aagtaaaaaa ggtgaaaaaa gtaagatgca gattataaca
     5581 gaagtagacg agaaaagtac aaatagaaaa catgagaatc aaatcatatg atctttttca
     5641 aaaac
//
LOCUS       DAYYHX010000170         5536 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000170 DAYYHX010000000
VERSION     DAYYHX010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.171"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(90..770)
                     /locus_tag="ACHA6Y_002331"
     CDS             complement(90..770)
                     /locus_tag="ACHA6Y_002331"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8988404.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            896..1765
                     /locus_tag="ACHA6Y_002332"
     CDS             896..1765
                     /locus_tag="ACHA6Y_002332"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8988405.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            1791..2306
                     /locus_tag="ACHA6Y_002333"
     CDS             1791..2306
                     /locus_tag="ACHA6Y_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM8988406.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(2353..2916)
                     /locus_tag="ACHA6Y_002334"
     CDS             complement(2353..2916)
                     /locus_tag="ACHA6Y_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8988407.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(2971..3150)
                     /locus_tag="ACHA6Y_002335"
     CDS             complement(2971..3150)
                     /locus_tag="ACHA6Y_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988408.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(3227..3736)
                     /locus_tag="ACHA6Y_002336"
     CDS             complement(3227..3736)
                     /locus_tag="ACHA6Y_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988409.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(3832..4212)
                     /locus_tag="ACHA6Y_002337"
     CDS             complement(3832..4212)
                     /locus_tag="ACHA6Y_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8988410.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            4542..5291
                     /gene="pnuC"
                     /locus_tag="ACHA6Y_002338"
     CDS             4542..5291
                     /gene="pnuC"
                     /locus_tag="ACHA6Y_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM8988411.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         1646 a         1071 c         1013 g         1806 t
ORIGIN      
        1 tattttatct gtatcagtca aaaatgctgg aaaaattaag tttttcacat acaaagcgaa
       61 ctgcactata acaggaaagc gctgttttat tacactgttt caggcagttg ccaatcaatc
      121 ggttcttctc ctaacgcaag gagcgcatca ttcgttttcg agaagggttt ggaaccaaaa
      181 aagccacgac ttgcagataa cggactggga tgtggagatg ttatgatgat atggcgactc
      241 ttatcgatca ttttgatttt ttcttgtgcc ggttttcccc acaagataaa cacgattggt
      301 ttttcccgtt catttaattt ttcaatgatc gtgtctgtta atctttccca acctttcccg
      361 cgatgagaat acgcttgccc ttcccgaacg gtaagaacgg tgttgagtaa aaggactccc
      421 tgtttagccc atgacactag atttccatgt tgaacaggtg cgattccaag atcatcataa
      481 agctctttat agatatttct taacgatggc gggatcttta cgccaggttg cacagaaaaa
      541 gataagccat gagcttgatt ttctccatga taaggatctt gtcctaaaat caccacttta
      601 acttcttcat aaggagtcaa ttccaatgct tcatagatat gatacatatc tggatggatc
      661 ttttgggacg catattcttt ttttaaaaat tctcttagat gcagataata atcttttgaa
      721 aattctgagg acaaaacttc ttgccaactg ttatgaatga tttctttcaa aaactccacc
      781 tcctaataaa aagaataact cacattttct tcaatcacaa gggacaatct tcaccctcag
      841 cacttgatta atatggtaaa ctaggaacaa gaagcagaaa ggaacgataa tcattatgat
      901 caaattaatt gcatcagata tggacggcac attattagat gcccatatga gtgtttctcc
      961 tgaaaatgcc gaggcgatac gttttgcaaa tgatgccggt gttgagttca tggtcgctac
     1021 tggtcgaaac tatcaagaag ctcgcgcagc tttagatgag gtaggaatcg attgtgcgat
     1081 gatcactttg aacggggcac aggtatttga taaagaagga aattcattgt ttaccgtgtc
     1141 gattccaaat aatcaagcaa tctctgtttt agatattcta gatgctaacg gtatttacta
     1201 tgaagtagca accaacgatg gtctttattc tgaaaatcaa gcgaaacgaa ttgaaagttt
     1261 tgcttccatg gtggctaccc atttacctca ccttacgtat aaaatggcga ttgccatggc
     1321 ctcagctaag ctggagttat tgcacatcac atacgtggac agtattcgtg ctattttgga
     1381 agaaaagaat ctggaagttt taaaaattat ttgttttcat acagaagggc cagctgtatt
     1441 aggtccagtt ggaaagcaga tttctctttt aggcgatcta gccgttactt cctctggtca
     1501 aaacaatatc gaagtgaacc ataaaaacgc tcaaaaagga attgctgttg cccatgtcgc
     1561 tcacgagcgt gggatttctt tagaagaagt catgacgatt ggcgataact tcaatgatgt
     1621 cagcatgctg caagtagcag gagtcagttt cgcaatggga aatgcagaaa tcgaagtaaa
     1681 agattatgcc aaatatacaa cggacacaaa ccttgaatct ggcgtaggaa aagccatatt
     1741 gcgcgcgatt gcagaagact tgtagtttta tctagaaaag agggatatag atgtcagaat
     1801 ttttcattca ggaaaagcag cttggacgtg caactcggac aattgtcaaa aacgaaaaag
     1861 gacattccct gtttttgatg gttggtcgct ggggaacaaa gggcgatgca ttgtctcttt
     1921 atgccatgaa tggcgatctc gttgctcaca tcaagcagat aagcctgaca ttcggtacgc
     1981 gttttgagct gtataaagag ttcgaaaagg tgggaaccat gcagaaaata ttcaattggc
     2041 ccggtgactt ttattatatt cgtagacttc actggacagt ccaaggtgat atctataatc
     2101 atcaatatca aatccatcat ttcaatcagc aaatcatgaa aatggataaa gcaacacttt
     2161 tttcaggaga ttactatgta ttggatgtct caaatgatga agatgctcca gtttgtattt
     2221 gtattgctgc ggttttagat tactggctct accgtaaaga caaaagtcgc gataagcctg
     2281 tttcttaccg acttaactta agttaaaaag atgtacctga aatgcggaaa tacgcatttc
     2341 aggtacatct ttttagatat tcttttgcat atgatcataa agatgaccac tgatcatccg
     2401 ctgaccgaca actttgaagt catggcgttc gtataatttt ttagcctttg gatttttttc
     2461 atccacactt agaccgatga cggagcgatt agctttcttc gccaatttag gtaatgcttc
     2521 taataatcgc gaaccgatgc cttgtcctct aaaatcttct gatacactga tagagtccaa
     2581 ataccattcg ttgggaaacg tttctttatc aatgaacaaa cgtgtagacg catctagctg
     2641 atatttctct aagataggta ccagtggctg atcgatgatt gattcttctt catctgtata
     2701 accgtaagca actcctgcaa ctttaccgtc tatttcttct acgatacctc gtgaataact
     2761 gtaacgataa tttggatcag tgattgcttc ttctaaaatc tctaaagtct tttgttcccc
     2821 tgtctgcttc aaaaatggca gttccatatc ttttagaatc actaagatca atggggcgat
     2881 ttctggagca tcttcttttc tcgcaaaacg aatcatagtt tcctctcctt ttctccattc
     2941 taacatgcag aaaaagaaat cgcaaaatcg ttattttaaa aaatcttcaa tcttttcagt
     3001 aatgaaataa ccatgagtaa cgattcttgc cgcttcagtt cggcaataag ggcagacgac
     3061 tggcatttct ttatcattat gttcgactac attattgact tcttcgtatg caaatttttt
     3121 tccgcaatta gggcatttca ttttttccat gttatctcaa cttcctttca tctttactct
     3181 cttagcttac tcttatattg taaaaaagag aagcaatatc cttcttctat gcagatatcc
     3241 atacctgacg gtcctgttct ccatctatta atcggacttt ataataagga cgttcatctt
     3301 tgactagaag tgtccactct ttcacttttg cttttttccc gtagtagctg actgcttcct
     3361 tcatcgctgc ttgcggttgt ttgaactctt ccgttttttc tatatcaaaa agatgttctg
     3421 cggccttttc cgtgtcattt cgtacggttg ttttcccagt ggatggattg actgctatga
     3481 cttgtccttc acaaaaaaac tggtattcat atgttgcatt cgtaggattc tccaaatctt
     3541 tttcaactgc aaaatgaata gcagatatgt tatgttcttt tgtttcgttt tttacgattg
     3601 ctgctgcttt tttcaatgag atccgatatt cctggtcact aatatcatcc atgtgaaaag
     3661 cggcattgag cagtcccgtc ataccggtaa tccctattac gatgagggca gcaacaccaa
     3721 acatcatttt tttcattctt cattctcctg tttctttttt ttaaagaata tcaagaaatg
     3781 aacgaatgaa aaatcgaatg aaacgaaaaa aaagtgacaa ttttgttatc tttattcgtg
     3841 cagttctaat ggcaatccgt ctggatcaaa gaaaaacgtc attttttttc ctgtaaaggt
     3901 atcttctcta atcggttctg attcgatccc taaggactgc aattctgcaa ttgtttcttc
     3961 aatatcgtct actttgaaag caagatgacg caagccacaa gcttctggat agtttggtcg
     4021 ttgtggcgca ttagcaattc caaaaatctc caattcagca cttcctagtt tcagatctaa
     4081 tttatagtca tctcgttctt tgcggtaatt ttctctgatt acttcaaatc ctaatttatt
     4141 aacataaaaa tcacgtgatt tctgataatc tgaaacaatg atggctatat gatggatcgt
     4201 tgtaaggttc atatgagcac tcctattctt cgtttaagct cggatctata ttaatctcca
     4261 gttccattgt aaatgtgtca gattgaaaaa aatagctgtt tttccttttt ataagaaaaa
     4321 aggcaaaaaa aacagaaagt aggtcctcca agacactctt cctgttccca atataataaa
     4381 tgaaagtaag aaattatatt ttcaagaaaa caaacagaaa attatcataa cacaaattat
     4441 ttataattga aagactagtt ttttttcaag aaaagcatac aatagattgt gttagtaaat
     4501 acagaacaca ctacatttag tgtcttaagg agtcctcata catgaagtca tattttcagt
     4561 ttttagctca tcagctaaaa ggctggccgc aacaaaatta ttatcttttc tttttcagtc
     4621 ttggctgcca ggtcatgact cttgtcaata gtcccatcac ttggctgtcc gttattacat
     4681 ttatcggtac cacgcttggt gtattgtgta ttttatccat caatgcggca aaatcagtca
     4741 acggtatttt aggtattttg tctgcgattt gttttattat cgttgggttt tcagcaaaga
     4801 actatttaag tatcggtgaa cagattgctt atatgatcac attggatatc cccgtattgt
     4861 taagtaaaga atggaatcat aatatggctt caaaaatccg aaaattcact ggaaaaagct
     4921 ggtttatcgc aatcatcagt actttccttg tctatcttgt ttcaggattc ctgattggtc
     4981 gttatacaga tgatcctcgg ccgtgggtag atgccattgc tttttctatt agcttgactg
     5041 ctggtatcat cagtttcttg cgttacaaca accaatatta ctggtggtta gcttcagggc
     5101 ttgctcaaat ggtcctttgg tatatttcat ttacgcatgg atctgcaagt ttggcgatgt
     5161 tcgtgaacag ctctgtttac ttgctgaatg atgtccttgc atttaccgtt tcaccttggt
     5221 ataaccaaaa agaacgtgaa cgtatgcaaa aaatggaaga agaatactac aacgcacaac
     5281 aacacgcata aaaaaactga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct
     5341 aaacacgtat aaacggcgga atcagaagca actccttcgg aaataagctg aaattcacaa
     5401 aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa cgcttctgtc
     5461 ccagcctcta tttcctacga ttagtcaagt aaaaggtgca agaaaaatag ctcgtaaacg
     5521 aaagcgacaa aatcaa
//
LOCUS       DAYYHX010000171         5481 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000171 DAYYHX010000000
VERSION     DAYYHX010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.172"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(121..396)
                     /gene="prgO"
                     /locus_tag="ACHA6Y_002339"
     CDS             complement(121..396)
                     /gene="prgO"
                     /locus_tag="ACHA6Y_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM8988412.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(368..1321)
                     /locus_tag="ACHA6Y_002340"
     CDS             complement(368..1321)
                     /locus_tag="ACHA6Y_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8988413.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1933..3426
                     /locus_tag="ACHA6Y_002341"
     CDS             1933..3426
                     /locus_tag="ACHA6Y_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8988414.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3561..3857
                     /locus_tag="ACHA6Y_002342"
     CDS             3561..3857
                     /locus_tag="ACHA6Y_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8988415.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3958..4809
                     /locus_tag="ACHA6Y_002343"
     CDS             3958..4809
                     /locus_tag="ACHA6Y_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988416.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4830..5042
                     /locus_tag="ACHA6Y_002344"
     CDS             4830..5042
                     /locus_tag="ACHA6Y_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988417.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1885 a          843 c         1029 g         1724 t
ORIGIN      
        1 atttgtgaat gttgtatacc cccaaacaat acacatattt tgaaggatta ggattaataa
       61 agctaaaaat atagtaagta tactaaatat atttactata tctagtatac ttactatatt
      121 ttaatctttt gatcgtctat tatatataat ccgttcctcg tcgtttaaag tatcaacata
      181 ggcgtccatt gcaagtttga ctatttcata cataggaata tcttttgcat aactaagatc
      241 ccgaatggtt tttagtacgg gaggatctac ttgaactggt tttctttctt tcgcagtgta
      301 ctttcgttcc cctaactcga cattaatatc atctttaatt gaaatatctt cagcaccaat
      361 tttcttctta tctttgttcg ttggtaataa tgccatttag tttaatctcc tttccaagag
      421 gtaggatatg attggccatt gagtcagcat atttaggtgt ataatcaaat ccttcaatat
      481 caccatgttg ttcatagtat tcaattcttt cttctaattc tgtaaagatg tctgcaaaaa
      541 tagcccacca tcttcgatct acataatcat ttaattggat accattctca ccatacattt
      601 ctaaccgatc cgagccttta acaatgcttt taaaaatgtt tgattcacca tacttatcaa
      661 ttgttttttg atagtttgcg tgttgagagg aacgtcgcac tgagaaaaga acaggcaaaa
      721 ttccaatgat ttgaagatga gactcttcaa aattcgtgac aatcggaact aaaactttat
      781 taataaaatc ttctgttcct tccattgcgt accgttttaa ttcttgcatt gcaataatat
      841 aatctgcagc agttaagaat gcacgaacca cattatctgt tgatggtggg cagtcgataa
      901 agatataatc gaaatttttt ctcaattctt ctaaaggttt aatgaaggcc aatgatctct
      961 tacgacgatc aggatatttt tcggacaagt attcattaag ttcttcagat ccaggagagc
     1021 cagccataat atataaatgt ggacttactt gagtaatacc tgaaacccag tccccttttt
     1081 tgattgctgc agacatggat cgatcaaagc tagtaatttg atacgttgat ccaatgttgg
     1141 aagtagtatt tctttgttgg tcataatcaa ttagcaacac gtctttttca aaaagttgac
     1201 tagaaacaat cgctaataaa tcattgtctg tagtcttacc aatgccgcct tttctacctg
     1261 taagcattac aacttttcct agtttatctg atgttttaaa tgcagtccgt aagttttcca
     1321 tagttccctc caatttccta tactaaatat atttagtata cttcatatac ttaatatatt
     1381 cgatatatct aatatactcc aaattgataa gaattcagac tttataagga atatagtgga
     1441 tatactaaat atatttgcta tattcagtat atcagatata ttcaaaatgt caaaatatat
     1501 ttgtgcgttt tgcgttgaca atttagagtg aaaagaggta aacttaattc tatagaaacc
     1561 acagataatt cactatttac aaagtagcaa aagcagtggt actctttaaa cagaaaaatt
     1621 acataaaaaa aagagacgga aataattgat tttagcagaa tcaattattt ctgaatcaca
     1681 tggttaggac atgtgttcac gctcgtctca agaatatgta agtagctacc aactacttat
     1741 attaaaagtt gatcggatac cttttccgat tgcctttatt ctaccaaaaa aaggcgagca
     1801 actcaacaga agaacgttta tttagtgtac ttaaattaaa tattcttaaa cactgaagag
     1861 agaacacata ctatggctga gattaaagct aaactgtgtt ctctttttgt ttgagaaagg
     1921 acgttacaat cgatgttgag cgaaatttat tcagctattc ttaaaaacgg cttacgtaca
     1981 tacaaatatc gaaacagcac catgaaacct gcaggtcata ttgattccag taggagtggt
     2041 gctatctttg cgtaccgtag caagcaatta atgaatgttg ggcgtggtat ggtcattact
     2101 tcggaagaag ctattttaga gaacgaaaag aaactaacgc attggacacc taatacctat
     2161 cgctttggta cttatgcgga tgattatcga acagtggtta aaggtcattc tgagaaaaac
     2221 ttaagtcaaa tcaacacatt tgtagttgat atcgattcaa aagagaacca tcaaggcgaa
     2281 attattttag cctgtttaga tcaagtgggc tacatgccaa cgctaatttt agaatcagat
     2341 catggttatc aagtttattt tgtactaaag acaccaggct atgtcacaaa gaaaagcaat
     2401 ttcaaggtga tcgatgtagc aaaaaaaatt tcaaaaacaa ttcgtctaca gttggctgaa
     2461 aaattgtccg gagtggatct aggatgtaac cacttcggaa ttgctcgatt cccacaagaa
     2521 aataatattg tttttaaaga aatgaactat cagtattctt ttgctgactg gattcaatgg
     2581 tcgatgaagc ttgaatcaga tcaaaaaaca aaagaagatc gagaacgaaa actaattgtc
     2641 tttcctgaga aaaaagaaat tcgtcaagta gatgaaccgt ggttcgatat gttacttcat
     2701 aaagcaaaca tcgtcggcgg agaaggacgt cttggtcgaa ataatgtgat tttcacgctt
     2761 tctttagctt attactcttc aggttatgca ttggatactt gcgaatacaa tatgttcaca
     2821 ttcaacgaac ggttacagga accgctatct gaagcagaat tagtaaagat tgtacgtagt
     2881 gcttattctg gtaattatca agcagcaagc agagaattca ttattgaatt gtgccagcaa
     2941 tgggtttcac caaccttaaa aaataatgat ctgttcattc aacgacgtgg ctggtggaaa
     3001 tttaaaaagc cacgagatca aagaaaatat agtcataaaa atgaatggaa agaagatcta
     3061 ttggcttatc taagcgaaaa aagtcttgct agtgagcctt atttgaacct gtcaaaaaaa
     3121 gaattggctt cacaactaac gatgcccaag cgaacacttg ataagatctt aaatgagtta
     3181 cgagaggaga ataaagtctt ctacaaggta acacgaggaa gaaatggcgg aatgatcatt
     3241 gcttcagtca aaacgttgtt cgcgagggtt attcaactaa gtaaagaaaa gagactagat
     3301 tattcaacaa ggatcacaga ggtatttgga ctcaacgaga gcttagtaga agagacattt
     3361 agacaattat caaccccctt gaaccctctt aaagaagtga atctttttga agtagataca
     3421 ggttgaaaaa taaaaggtgc aaattgcctt atacaatata tctatgatac ttctgtaggt
     3481 gttgctccct ttgcctagct acgtgtaggt ggtatactta taactgcgta tgcagtctta
     3541 tgtatcatgg aggaagttgt atgtcgttaa aaaattatgt ctattcacat cctgttaatg
     3601 tgtttatcat tggtcgtttg ggattatctg tggaagtgtt ctgtgagctt tatggtttta
     3661 agcaggggac tttgtcttct tgggttacca gggaaaaaac agttgcttct ttgccgattg
     3721 agtttcttca tgggttgtcc ttagcttctg gggagactat ggatcaggta tatgattgtt
     3781 tgtgtgtgtt agagcaggag tatattgagt ataaaatagc taacgagtta agaaaaagga
     3841 agaagtatat ccagtagtag tttttattta cttaaaaaat tggttcattg agcaacctat
     3901 cgctgctaaa gtagcaaagg aaaacgttaa aggaaagaat ttagggaaga aatcgaaatg
     3961 aaatcggacg actggattga acacacctgg cttcctctga aaagaaatag caaacgaaag
     4021 aaattttggg tattgcaatt atggccatta ggaaaacttt gtagttacgg ggcacgactt
     4081 attcttgatt taaccgttta ctcgattgga gtatttggaa cggcttatta cttgataaat
     4141 gatcccaata ggattgcagc atcagacatt tttacagtgc taatgacgtt gatttttgtc
     4201 atgctattgt ctatttttac tcaattagtc atgcaattta ccaggcaaaa attaacagca
     4261 ggaaaacttt tcaaaagcct attcgattta attgttgtaa atgcaattct agcgacttta
     4321 ataaaacaaa ttgaacaatc tggaagaaca acaaaaggag tgaaggattt tttattgacg
     4381 tttattcact ttgtaaatgg acacatgtat ttgtttgaag tgggtattat tctctttttt
     4441 gtcgggtatg tatccacgaa acttattaaa cgttcaattg aaaaagaaat tgaaattgac
     4501 ctgattccga cacttcttgg ttatgagtac aatgttgatt ttggaagata tgacgaattg
     4561 atcaatacgg atatcgttca cacgagtatc catgaatcta ctgttaaggg catcagatct
     4621 attttaaaaa atggccagga aatgaaagca atagatcgaa gaaaagagat ctacaatttt
     4681 tatgaagtcg aagcaagcct acgatatggc atcggaaaat tgtggttaga tggaagtaat
     4741 attcgtcaaa acaaaccata cattttttgg aacagtagtg atttagaact tccaatgaaa
     4801 gataaataat tatggagaat cgtttgaaaa tgattcctct atacaggatt gtgcttatag
     4861 ttggcatgga gttaaaaacc ttaaagaagg agcagttatt caaagtgaaa gaacgtcata
     4921 atcagttact atgtagtaaa acgggtacga tgaacattga ataccgaaca gaatatttag
     4981 aattaacgaa tcaaactgat tttttgttga ttcgaaagca acaagagcaa gaaaaaaatt
     5041 aatggctact tatagccaat tgattgatga atgggaacaa ttcaaggaat ctaaaattcc
     5101 agaacgtctc taaaagcctt tatttgtaaa gagcataagg aaacgtggga acacttttta
     5161 aaaaaagtgt tccaataggc tgtgcctatt acgtttctca aaaataaatg tgggaacaaa
     5221 atatttaaaa ataggatagt gaaaggatag tgaaggatag taaaaggagg gtagtaggat
     5281 agcaaaaagg ataggctcct tttcgctatc ctttttaaat tggaggaatt aatatggaag
     5341 taagaataag aaatgtggat gagaaaatcg taagagaaat tgatcgaaga tgtgacgaac
     5401 ggttctgttg caaagtttta aatctactat caaataaggt agaataatag aaaaagatag
     5461 caggaggaat gacgatgaat c
//
LOCUS       DAYYHX010000172         5436 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000172 DAYYHX010000000
VERSION     DAYYHX010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.173"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..131
                     /locus_tag="ACHA6Y_002345"
     CDS             <1..131
                     /locus_tag="ACHA6Y_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFM8988418.1"
                     /translation="ITSISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            307..1050
                     /locus_tag="ACHA6Y_002346"
     CDS             307..1050
                     /locus_tag="ACHA6Y_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8988419.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            1043..2623
                     /locus_tag="ACHA6Y_002347"
     CDS             1043..2623
                     /locus_tag="ACHA6Y_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM8988420.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(2874..3353)
                     /locus_tag="ACHA6Y_002348"
     CDS             complement(2874..3353)
                     /locus_tag="ACHA6Y_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8988421.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3369..4121)
                     /locus_tag="ACHA6Y_002349"
     CDS             complement(3369..4121)
                     /locus_tag="ACHA6Y_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8988422.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4238..4308
                     /locus_tag="ACHA6Y_002350"
     tRNA            4238..4308
                     /locus_tag="ACHA6Y_002350"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4270..4272,aa:Gly,seq:ccc)
     gene            4669..5394
                     /locus_tag="ACHA6Y_002351"
     CDS             4669..5394
                     /locus_tag="ACHA6Y_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8988423.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
BASE COUNT         1756 a          952 c         1042 g         1686 t
ORIGIN      
        1 acattacatc tattagcaga gtaaacgaaa tagtaacatt tgcaaatgga gaacaactag
       61 agatgggaaa gagaagtatt gataaactaa agaattttat gcaagaaaag atagaaaatg
      121 aatcatttta aagaaaacaa agactcatat cttacctgtt agaagagtgt tttcctgtag
      181 aagagaacaa cccaagtagc agcctagtcg tttctatcct tttttgttat gcactctttt
      241 tgacatttac tccaccaata acgaagggta caataaaaag taataacaaa caaaaggagt
      301 acagaaatga ttgaattcat caatgtaagt aaaagttacg ggaaaaaaca agcattaaaa
      361 gacttgtctc tctcgataag acaaggggaa atttttggat ttttaggaca taatggtgcc
      421 ggaaaatcaa ctacgattaa aagtttagtt agtattattg aaccatcatc gggaacgatt
      481 ttagtagatg gaatgaaatt gacggaaaat cgattgagta tcaaacaaaa aatcggttat
      541 gtgcctgatt ccccagatat ttttttacaa ttgacggctg gagaatattg ggacttgatt
      601 tcagctgcct atgaattaga cacacaaaaa aaagaaaagc gtttagcaga attgacagcg
      661 ctttttgata tgtatagtca tcaaaatgaa acaatcgcaa gtttttcaca tggaatgcgt
      721 caaaaaacga ttttgatcgg cacgttgctg ccagatccag atatttgggt attggatgaa
      781 cctttgcaag gacttgatcc tcaagcagca tttgatttga aagaaatgat gaaggctcat
      841 gccgcaaaag gaaaaacagt cattttttct acacatgtat tggataccgc acaacagtta
      901 tgcgataaac tggctatttt gaagaaaggt gaattgattt atcagggggc agttagtgaa
      961 ttattgaatg gctcacctga tgaaacgcta gaaaagattt atttgaaaat ggctggaagg
     1021 caagcgacag gagatggacc gtatgtataa gcgacaatta ttagaattaa ttcgagttaa
     1081 tcttttatat gttaatccac agctcacgaa taaagcaaga gagaaaggaa aaaagggaaa
     1141 gcgattaact atctatcttt tgtctcaaaa tcttttttca gggctgattt ttttactgat
     1201 ttacggattt gctatgtttg ctatgaactt tgttaaattg ccaggtttct ttacttatta
     1261 cgtggcactt tttggtatgt taggtttaac tcaaagtata tcagtcattt ataatatctt
     1321 ttttgaagga aatgacctgc aagtattttt gcctctgcct tttggacaag aacaggtttt
     1381 ttcagcaaag attttggtag ttgctttgac catcattcct tttgtttttc cgcttctagt
     1441 catgtttatt ttaaccggat ggcgagctga tctagggatt ttgcttacta tcattatctc
     1501 aatccttttg ttttctctat ttttgattat tcttttttgt ctggctagtt tgattgtttt
     1561 tggtttggct cgaacagcag tgtttaaaaa acatagaaaa acagtttcta gtatcttgct
     1621 tggcagctcg atgctgatcg catttgctgg aatcatgtgg atgaatcatc aaacggggac
     1681 tattgaaaat gagttaacag atcggcatcc gatttcgata ttattgccat tctatgatgc
     1741 tgccagtcag ccactttctc aacaaggttt attgggttgg ggaatactag ctatcacggc
     1801 tctgggattt ttccttgctg taaaacagct gatagtacca aagcttacag agcaactgac
     1861 tgattcaaac agtgagttca aaataaaaag aacccataaa aagaaccaaa atgtgcatca
     1921 gcttttattt agttacaatg ctcaattagt aaaagaacct aatttgatta tgcaagttct
     1981 ttcttcttcc ttgttaactc caatcatttt tattcttgct tttgccctag gaggcgaaat
     2041 aaaattaact gatttagata ataagttaat cggtgttgtt ttcctggtag ggatcgcatt
     2101 ggctttctta acagttaatc agacttcttt tatcagcaat cttatttctt tggatcaaga
     2161 aaattttctg tttatccgtt ctttgccaat ctctatgaac caatatttaa aagaaaagtt
     2221 cagattcgga tggatattgc agagtttttt aactggtggg atcgcattat taagcggact
     2281 gtcttttcag ctgcctttgt tttttatccc tagcttgcta tttggttctt tattcggctg
     2341 ctacttattg tcactacgtt atttcgctag agattatcgg ttgctgctgc tgaactggac
     2401 caatattaac caattattta acagaggttc aggaagtttg ggtttagtcg caactatgat
     2461 gggttcattg atcatcagta ttattcttat cgtcttatac ggaatgttgg ctttgtttta
     2521 tccattttgg ttagtgaata ttcctgtgat cggtttagtt ctaatacttt ccgctctatg
     2581 gatcgtttat tatcaacgaa cgttttggaa aaaatttgaa taaagaagag gggccgtgac
     2641 aaaaatcatg tcgcagcccc ttttttacag atagagggtg ttcaaaagtc agtttctcag
     2701 tattaagtca attttatgta aaaatgtgag aagctatttt tacaaaactt tttttaatac
     2761 tcataattac ttcttattgc tcctgcggtt acttgtcgca atgtttgtga gatattttgt
     2821 catcgctcaa ggctgaacgt ctctttcaca accttttctt tgttacactt cgtttattta
     2881 aaaagccgtt tttttcgttt catcatgatt gcgtcacgct ttactttgaa attacggatg
     2941 ttatggttaa agacttctga taatacaaga cctaaagcga ttgctccagc aatcataatt
     3001 gcttggaccg cataatgagc cgcttctcga taggattcag taacgagatt tcgaaccgct
     3061 tgataagcta aaccaccagg gactagagga accatcccgg gaatattgaa aatcgttacg
     3121 ggcattttca aacgtttaga gaaaagatcg ctgatgaatg ctactcctaa agaaccaagc
     3181 aatgaaccaa aagcagcaga tccgcctatt tgaacacaga tccagtagat catccagcca
     3241 aaagcaccag aaagaccaca acaaatcaat gaacgcctag gtacatttgt aataacggca
     3301 aatgccgcag tcgctaaaaa actaaaagaa aactgaataa atatcgtccc catacttgtg
     3361 ttcctttcct aataaaaaag ctggaaaata aaagcaatcg caaaaccgat catcgtagca
     3421 gtcatcatcg cttctgttcc gcgagaaact cctgagacat aatgtccagc tagcaaatca
     3481 cggatagcgt tcgtaatttg aactccagga actaaaggca tcacacatcc gataatgatt
     3541 agatcctgat tgcttcctaa gccaaaacgt acgctgtaga tagcggctat gccaatcaat
     3601 aaagaagata aaaattccga taaaaatttg attctaaaaa atttcaaact atatagataa
     3661 gctgcgtatc ccaatcctcc aatgactaaa gtcacaggta aatcggtcat ctttccgcca
     3721 aataacacca taattgttcc actgatcact gccgcgctaa cgatttgcaa ccagacagga
     3781 aagaaagaac ggtctcggtc gattgcttgc agttcgcagt aaagttcttc taatgttagc
     3841 tctgaagcaa catatttacg tgaaagatca tttactttta ctactttttc taaattaatc
     3901 gaacggttgg taatttgttc cattcggatg gtcgaggtac gatcaagacc aataaagaga
     3961 ccggtttgag tgacatagct gaccaaacgg aaatttccag aagctaaagc aattcgactc
     4021 atcgtatctt ctacccgata catctccgca ttgctttcca tcatgatttt cccagcgagt
     4081 aaacaagtgt ccagcaacag atctaattct tttcccgtca tagcatcttc tccaataatt
     4141 gattgattag ttttatttta ctacaaaaca taccagaagc gtaaagaata aaaaaacttg
     4201 aggaaggaat aaaagaggcg tatactgttt ttgtattgcg ggtgtagttt agtggtaaaa
     4261 tcccagcttc ccaagctgat gtcgcgggtt cgattcccgt cacccgctta aaataggttg
     4321 tgaaaaagct gctctgcaac aagtaataag aacagccaag caaaaacaac ttcttatgtt
     4381 tttaacttgg atggacttat taccgcagat gcaagctttt gcgcaccgtt taccaagcgg
     4441 ttgtgaaatc agtgtattgt cttgagtatg aagaaaattt ttgaataata ataaatacct
     4501 tacagataga gtggtgactg attagagacg aggaatccgt ctcttttttg tatttttaag
     4561 ccatcttaaa acaggtttta ttttttgctg tttctatttc tttctagtat ttatttatag
     4621 gggaacatat gttaagattt agggagctgt tagtaaagga gtaaagaaat gagcaaaaaa
     4681 acaaccaagg cgatcgtggg gatcgtcgct gctgtgattg caggggcatt cggtgtttcc
     4741 accacacaaa aagattcaga tatctttcag cagattacaa ctatttttga ttccacacca
     4801 caagcttctc aagtagcgga tgtgccagaa tatgatggaa aacatcaaga aatagaaatc
     4861 aatgggaatc agccggaatt tacaaaagag gatcttagcc tttccaaagg aacatgggaa
     4921 agctattcaa atattgataa attgaacaga gtaggacaag ccaatgctat gcttggaaaa
     4981 gatttgttcc ctactgaaaa gagagagcct ttgtatattg acccgacagg ttggaaacaa
     5041 aagaaattaa gtgacgggca atggttatat aatcgcagcc atttaattgg tttccagctg
     5101 actggtcaaa ataacaacat taaaaacttg atgacaggga cacgttcgtt gaatgctcca
     5161 tacatgcttg ctcacgaaaa tgatattgcg gcatatatca aagaaacaaa tcatcatgta
     5221 cgctaccgtg tcactcctca ttttgagggg aatgaactgg tagccagggg agtgcaattg
     5281 gaagcagaaa gcattgaaga caaaaaaatt gaattcaatg tctttatcta caatgttcaa
     5341 gatggctaca cgatcaatta tgagacagga caagcaacaa aaacaaaaaa ttagtagcag
     5401 ctattcgaat tttttatgaa tattatgaaa aaattg
//
LOCUS       DAYYHX010000173         5434 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000173 DAYYHX010000000
VERSION     DAYYHX010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.174"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1506)
                     /locus_tag="ACHA6Y_002352"
     CDS             complement(1..1506)
                     /locus_tag="ACHA6Y_002352"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM8988424.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1598..2188)
                     /locus_tag="ACHA6Y_002353"
     CDS             complement(1598..2188)
                     /locus_tag="ACHA6Y_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8988425.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2367..4013
                     /locus_tag="ACHA6Y_002354"
     CDS             2367..4013
                     /locus_tag="ACHA6Y_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFM8988426.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4062..4781
                     /locus_tag="ACHA6Y_002355"
     CDS             4062..4781
                     /locus_tag="ACHA6Y_002355"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8988427.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5260..>5434
                     /locus_tag="ACHA6Y_002356"
     CDS             5260..>5434
                     /locus_tag="ACHA6Y_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8988428.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLA"
BASE COUNT         1636 a          989 c         1101 g         1708 t
ORIGIN      
        1 ttattcttca atcaaccgct tcacgattgc ataatcccct tcataaggtt catctacacc
       61 attgattttt tgatagggat ccacgccttt tcccgcaata acgactgaat cttgcggagc
      121 gctttctgct aaagcgcgtc gaatcgcttc aggacgatcc atttcataat gaacgatcaa
      181 atctggatta gtaattgctt cattgatttc ttccgcgatt tttttcggat cttcaaatgc
      241 tgggtcatct gccgtcaaaa aggcgatgtc agctgtttct gatagcactt ttccaaaatc
      301 atgtcgacgg gagattgctt tgtttcctgg gctgcctaat acaacaatta cacggccatc
      361 tggatgttca tttttggcaa atttcagcaa tgttttcaag cttagataat tatgtgcata
      421 gtccacatag acatgcgctc cattggattg gatcaattga tccattcggc ctggtacacg
      481 agcttctttt aatccgtctt gtgcatcttg actggaggca cctgttagtg ctgcggcaat
      541 gatcgcactg gctgcatttt cttggttgaa tcctccagcc agcaaaatat cataagcagt
      601 atcgcagata gctaagcgat cttcatgaga agataaagta aaaccgtgag tacctttatc
      661 tgaacggatc acttgataat tgctttcttc cgaacgacca taagtgatcg ttggaatctg
      721 gaatagatcg gctgtttccc taagtaaatc aaaatagtca ctttcatgat ttaaaacaac
      781 tgtttttgaa ttcaagatca attgtcgctt gcaatagaaa tagtcatcaa atgtcggatg
      841 ttcgatcgga ctgatatggt caggcgagat attcaagaag attccgacat ctaaagtcaa
      901 gccgtatacg cgttgtgttt tgtaagcttg tgaagaaact tccatcacta gatgagtcat
      961 accattttca actgcttctg acatcatacg atataaatct aatgactcag gcgttgtcaa
     1021 atgggatttg aaatatgttt tgccatctaa cgttgtattc atagtagaaa gcaatgctgt
     1081 ttttttattt gttgtgtgat ccaagattgc ttttgtaaag tacgcagcag ttgttttacc
     1141 ttttgttcca gtgaatccga tcactttcaa tttattttgc ggatagtcgt aaaaagccat
     1201 agcaagaagc gccattgctt tacggatatc cgttacgatg attcctactg ctgaattttc
     1261 gtatggttgt tctgatacat aatattgaat accctgttcg attgctgaat ccaaatattc
     1321 ttctttgaaa ttcattcctt tacagaaaaa caaagtagaa gcatcagctt ttcttgaatc
     1381 ataacttaac tgaagaaatt cagctttttc tggtacatca agatgccagc ctcttgttga
     1441 aacaaattct tttaatagat tttcttttaa tagtaaatga cggatgtctt ctaaagagaa
     1501 gctcattgat tgatccctcg ctttattcaa tttcttggtt tattatagca cactcttttt
     1561 aatatatcgt ttgtttccgc tatgacaaaa tccagcttta ttcaaatttt cttgacacag
     1621 gggacaagcg gatgagtatt ttcataaaca atgaactgac tgtaatagat aggagaacga
     1681 atagtgtctc caacaatcca aaaacataat aattcaaaaa gacgataaga ctattggtaa
     1741 gtaaaagtcc ttgcggcatt ggaagccgaa aaaatttgtg gaaaataatc ggcggtatcg
     1801 ttataccgcc atttgaggcc ccgactgtgt acaatgtgta aaccccaaga ctgaacaaaa
     1861 gacttcctga aatcagtgaa aagaaatgtg aggagatcat ttccactttt ggaacaatct
     1921 ctaaaacaac aggaaaaagc agacttcccg ctacagtctt aaaaaatact gattttccta
     1981 ataggacagc ggctagaagc agcatcataa gattgatgct ccaaacagta actgagcgtt
     2041 ggatagaaaa ggccgtttca actaaatgtc cgatggcgcc cactccggcg gaagctatat
     2101 cgtgagggag caagaaccaa ttcaatccaa ctgtgataaa gaaaagacct aaaaatataa
     2161 cgggtagttt ttttatttct tgtgtcattg atatctccta taaatgttag ccataaaagg
     2221 ctgtatattt ctttacttta ccaaaaaaac gaaaccgaat tcaaaaaaac aattgttctt
     2281 ataaaagtta tataaaatcg attgccagtt tctgttttac caattaaaat gatagtaatg
     2341 ttttgtagaa agttggaatc tgaccgatgg ataatcaaag aaaaccagca ttagatcaat
     2401 taacaccgca agaaaaaatg gcacgaggct ctgcttggat gaccgccagt aatatcattt
     2461 caagattgct aggcgctgtc tatatcattc cctggtatgc ttggatgggc gaaaatgcta
     2521 aggctgccaa tggattattc aatatgggat acaatatata tgcattgttt ctactgatct
     2581 ctacagcagg aatacctgct gcgattgcaa aacaaactgc acgctataat tcattgaatg
     2641 aatatggcac ttcacgacga ttatttatcc gtgcattaca gatgatgggc gggttaggcc
     2701 tgttgtttgc tttgttcatg tacatcgcct ctccttggct agctcatgct tctggtggcg
     2761 gagaagaact gattccaacg atgcgttctt tgagtatcgc tgtattggtt tttccttgta
     2821 tgagtgtgat acgagggtat ttgcaaggca atcaagaaat gatgccttat gcgctgtcgc
     2881 agattgtaga acaggtggca cgtatttttt atatgctatt ggctacattc atcatcatga
     2941 aagtcatgga tggtgactat gtgacggcgg tcacgcaatc aactttcgcg gcatttatcg
     3001 gtatgctagc aagtattctt gtccttttgt attttttgaa gaaacatcaa gcgtatacat
     3061 cggcgtttat tcactacagc gaaaacaaaa taaatattgc aacgaaggaa ttgttgttag
     3121 ataccgttaa agaagcgatt ccgtttatta ttgtgggttc aggggtaacg atcttcaaat
     3181 tagtcgatca gttcactttt atgaagatca tgtctgcatc caccgagtat tccaatgcac
     3241 agttactgga tcttttttcg atttttagtg ccaatccaga taagttgacg atggtagtta
     3301 ttgcattggc aacatcgatc gcgtctactg ggttaccgtt gattacagaa gctgttacgt
     3361 tgaaagatcg agtaggtctg gcaaaattga caagcggaaa tttacagttg ttttcatttt
     3421 ttatgtttcc ggcgacattt ggtgttatgt tacttgccta tccgctaaat acattatttt
     3481 atacaccgga ttctctagga agtaatgtac tgatccaagc aagttttgta ggactcttcc
     3541 taggactata tatgctagtt tccaacatgc tgcaaggaat gtttgaaaac aaggcggcaa
     3601 ttaattattt ggttattggg tttctagtga aactccttct tcaataccca gccattcgtt
     3661 tatttgaagt atacggccct ttattggcga caatgatcgg atttgctgtg tcttgtacct
     3721 tgatcttaaa gagaatccat gaagtagccc gctttaaacc aagttttgtc tggcgcagga
     3781 cattgctgat ctttattctt accttgatta tgcttttggc ttcgtggatc acaaagatga
     3841 tttttggtgc ttttttaaac gaagatagca aattccagtc tctattgttg attcttgtag
     3901 tagcaggtat aggggcttta gtgtatggtt atttagcttt gaaaatccgc ttggcagaaa
     3961 gactactagg accaggaata gcgcgtttac gacataaatt aaaaataaaa taagtgaatc
     4021 agaagagcta gattgatgct cttttgaaat gggggaaacg catgcgttta gataagtttt
     4081 tagcagatat gggacttggc agccgcaaag aagtcaaagg attaatcaaa aaaggaacga
     4141 tcgaagtcaa tggacaagtg gtgaaaagcg ataaagcgca agtaaatgaa ttcgaagatg
     4201 cggttactta tttaggagaa atcattacgt atcaaaaaga tttttactac attttgcata
     4261 aacctgcagg agtcatttct gctactcagg ataatcatga tcaaaccgta atcgatttat
     4321 tggaagacca ggattatcgg gaagatcttt ttccagtcgg ccgattagat aaagacacag
     4381 agggcctctt gattttaacg aatgacgggg tgtttgccca tcagttacta tcacctaaaa
     4441 aacatgtgga gaaagaatat ctagctgagg ttagcggtat aatgacagaa gaagatatcc
     4501 gtttatttgc agaaggactg attattgatg gcgatgagca gacattgccg gcaaaactcc
     4561 aaattgatca agtagatcaa gagacagaaa cttcaattat ccgtctgatt ttgcatgaag
     4621 gaaagtttca ccaagttaag cgaatggtca aagcagtagg aaaagaagtc ttatatttaa
     4681 aaagaatccg tatgggaaaa ttttggctgc ctaaagattt gaataaaggg caatatcgac
     4741 ccatgaccaa agaagagcta gaacaagtga gagaaaaata attgaatgga tagctaaaag
     4801 aataagcaga gtagttgtag cgccatctga aaagcagact tgtccgtctg actttcggat
     4861 ggcttttttg tgaaatttcg gcttatttcc gtagaagcta cttctggaac accgtttgtt
     4921 cggaataagg gactgtgaca agacttttgt tacagtcccc attgttcctt gtgactcaga
     4981 gttagatagt aaggacacga tctccctaat actcaggcca aaacgctgaa ttcacaacct
     5041 ctgataaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg
     5101 aggaactatt ttcccgtttt tttctctaat actcaggcca aaacgctgat ttcacaacct
     5161 ctcctatact ttgtttcatt ttccctatac tttgtttatt tattctcatt attgaaagcg
     5221 ctataattac gttgcgaatg aatcgaaagg ggtttttaga tgaaaaaacg aaaggggttc
     5281 aatcattttt ttatgaatct atggcgttat aaagcacttg tcctgatggc tgtaccgggt
     5341 atgctttgga tgattttttt cttttacata cctgtaatgg cgaatgtcgt agcattcaaa
     5401 aattttcata tttcagcaga tgggttcttg gcta
//
LOCUS       DAYYHX010000174         5391 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000174 DAYYHX010000000
VERSION     DAYYHX010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.175"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..474)
                     /locus_tag="ACHA6Y_002357"
     CDS             complement(16..474)
                     /locus_tag="ACHA6Y_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8988429.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(487..1818)
                     /locus_tag="ACHA6Y_002358"
     CDS             complement(487..1818)
                     /locus_tag="ACHA6Y_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM8988430.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1829..3271)
                     /locus_tag="ACHA6Y_002359"
     CDS             complement(1829..3271)
                     /locus_tag="ACHA6Y_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8988431.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3258..4022)
                     /locus_tag="ACHA6Y_002360"
     CDS             complement(3258..4022)
                     /locus_tag="ACHA6Y_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM8988432.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4126..5001)
                     /locus_tag="ACHA6Y_002361"
     CDS             complement(4126..5001)
                     /locus_tag="ACHA6Y_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8988433.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5256..5340)
                     /locus_tag="ACHA6Y_002362"
     tRNA            complement(5256..5340)
                     /locus_tag="ACHA6Y_002362"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5304..5306),aa:Leu,seq:caa)
BASE COUNT         1635 a         1138 c          807 g         1811 t
ORIGIN      
        1 ttttttcatc gtacactaaa attcaaaaca actatcttta atctttactt cttggttttg
       61 ttttaataaa tttccttcaa aaatgcctgg agaaactaca atgatcaccg tatctttttg
      121 tgcttgctcc aacttcttca aatctataat agctaacagt gtcccttctt ttacctcctg
      181 gttactttca acccgtatct gaaacggact tccttcgagt tcaactgtat ctaaacccat
      241 atggatcagt atttttctgc cagattcact ttcgatcgtt atggcatgtc gggtaggaaa
      301 tatattatcg attattcctt taactggcgc atggatatat ccatcatgat tcgttatagc
      361 gtatccaact cctatcattt ttgaagaaaa gacctcgtct tttactgctg ttaatggaat
      421 aatttgacca ctagctacag cttttagaat gtttcttgtt ttaaagaaat gcacttttta
      481 tcacctctac tttaatgctg gccaatattt tttgtttact ttgatcattt catctagtat
      541 tttttttgcg actctggcat ttggtacggt ttgattcaat gtaaatgctt gtaaagcttt
      601 ttgataagaa ccttcaaagt aagcatctac tagtaacttc tctactgcaa cttgtgcctc
      661 catcaatcct ttatgaaaat cactgattgg ttttcttagt gtaattggct ccacaccagt
      721 tgcaccaaca tatgccggta tttcaacaac tgcatctgac cgtagattcg gaatcgcccc
      781 ttgattagga acgattagca taaagcgacg acgatcatca ttcaacaagg aaatagctat
      841 atcgacgata tattgtccat gctccccaaa attaaagttc aatacttctt ccacaatacc
      901 gcggcgtatt ttatcagcca tttctttggt atttttctct cgtccatcca tgaccatatt
      961 agcacgtgta tactctttgt cagtatgctc aacgaccata tcaggatata agtaatactc
     1021 taagtaatta ttcgggatat tacttggaaa atttttcgtg atgactgaca tcatttgaaa
     1081 ggttttttgc caagttttat ctcctatatt aaaatcggcg acttgcattt cttttgttat
     1141 tagtttttca ataatctctg gcataatttc tcgttgcaat tctttatcat aaatcgatgt
     1201 ataccagcca aagtggttta aaccataata cgtaacgatc caattcttac gatcataacc
     1261 gtaattcact gcaatcgttt cttcaattga aatagtcata tcacaggcat taatcatttt
     1321 tacattcgga tatttacgac gaacagcttc agaaacgatc gactctggat ttgtataatt
     1381 caaaatccat gctttggggg catagtcttg tatatgatcg acaatttcca ataaaccttt
     1441 gattgagcgc atcccataag caaatcctcc gagtccacac gtttcttgtc ctactaatcc
     1501 atgtttaagt ggaatctttt cgtccttttc tcgcatctcc aatcctccaa cacgaatttg
     1561 tgagaaaaca aaatcgcatc ctgtaaaagc aagctttgga tcatccgttg cacgaatgcg
     1621 aatatttgaa aaaccttttc ttttcagcat aaatttaaca atcaagtaca tatccatatt
     1681 tctttcttca tcaatatcat ataaacgtat ttcagataat ggcaaccgtt tttcattgtt
     1741 caaaatggct tgaacgattc ctggtgtata cgtactcccg cctccagcta tagtgataat
     1801 ctgttttttc atcatcatct tttcctttct attcttctaa taatttattg aattctgcca
     1861 taacataagt cacttctaag ccgatgataa tttgataatt tttttcagtt ggtttaatga
     1921 tcccgcttat ccctaattgc ttgattcgct gttcctcgac catggtggga tccactacct
     1981 ctacccgtag gcgtgtatta caactataga catccaggat attggctttt cctccaaacg
     2041 cttgtagcat ttttgttgcc atatattcat aatttttact tgctaaccct aactctgttt
     2101 cttctaaggt tacttcattg ccaaactctg ccttagcttc acgtcctaat gtctgaatgt
     2161 ttctcttatt gatcaaataa taaaaagtaa cgtaatacaa ggcaaaaaag aaaattccga
     2221 ttggaagaat caaaatagca ttggtagcga ttcgaaaatt gatgagataa tcaacaatac
     2281 atgctccgaa tgtaaaacca attcgtatat tgaataagta acagacaatc cctgctaaac
     2341 cggcaaaaag tgcatggatc acgtacaaca taggagaaag aaacatgaaa ctaaattcaa
     2401 caggctcagt gatatttgca ataaatgaag ttcctgcacc gcttcccatg attcctttga
     2461 ccatctcttt attttcttta taagctgctt tatagatagc tagacatata gctggtatac
     2521 caaacatcat tactacaaaa aaaactgata aaaacagtcc agcctgagga tctccgttga
     2581 taaagcgtgt catctctcct ctgaaaactt ccttgctagg agatgtatag cttcctaaat
     2641 cgtaataaat atacgtattc aacacatggt gcagtccaaa ggggatcagc aaacgattca
     2701 aaaacataaa tatccccaca cctaatgctc ccatatcaac gagcagtttt gcaaaattat
     2761 caatcccaat ttggatgagc ggccagataa agcccaaaaa aacagctgcg attgtttgta
     2821 gaatcatcac catagtcaat gggaattttt cgcccgcaaa ataagaaaaa actactggta
     2881 atttttgttc tttaaagcga ttatacgtcc atgccgctac taaaccggaa ataattccac
     2941 caaaaatccc catattcact tctgggtcca taatttctaa gccttttttc aaggtagcaa
     3001 tcgctaaaaa accagtaagt gcaggtattc ctttgtcctt atttttgaca aaaccgattg
     3061 caactcctat agcaaataag atatccaagt ttccaaaaac ggcatttcca gctgcaccaa
     3121 ttactgggat gttcagcata tcgtcagctg ataaacgcat gatcaatccc gctaatggca
     3181 tcgctgcaac tggaagtagc atcgctcttc caagcttctg cacatttgat ttaacattca
     3241 tttttattcc tcctctatta aatatcctca tatgttacta actcaatcaa aggatcattt
     3301 ttcacttttt gagcaaatgc tgaatgaatt aaaaattcga cttggtgggt acgaggatac
     3361 acaaaacttg aaccatttaa caattcctga tccaaatacg ctgcgtgaca catgacttcg
     3421 atcgttttat cttctctcaa tatcatgagt aaacttgcca aatattcttc cacactcgta
     3481 ttgatttgtc gttttttttc atcagtttct cctacgacat cgaaacaagg ctcaaaataa
     3541 tcgacatgac gaacttcatc ttttcgttca aagttgctcc gaaccggcaa atcatatttt
     3601 tttgctaatg taacaactac ctcctgattt tcatccaaag tatgagtatg atgatgactg
     3661 tctaaatgag ttggtatgat ccccgcatga taaatttttt gaatctgagc attccattcc
     3721 tgatacagtt gatctttgtc aatgttaaat ggatgctgat aataagataa ggatctgaac
     3781 tctccgcttt cgataagagt atcgacagtt ttgaggagtg gtttgccaca cgtcaatgtt
     3841 aaatggactc ctacacctag cctaggcatt tcctttctta gtttcacagc atgctcgaaa
     3901 cccggcatat ttgccatcaa tgtagttgat gtcaaaattc ctcgctgata agcatccata
     3961 atcccataat tcacaccatg gctatagcca aaatcatccg aattaaaaat cacttttccc
     4021 atttcattct ccttcttttt tctactaaaa taggcgcaac caaataatca tagtccattt
     4081 actatgtgtc atttggttgc gcctatagat agttacgccc ttattttaat agattcttgc
     4141 tagatgaatt gccaaataga tgatttcatt ttcttgaatg gcaagttgaa attgctgttc
     4201 gatgatatct ttaatcttta gtgctgtaca atacgctttg ctatgtttaa atgtttcagt
     4261 aaaaaaacca atattttcta ctggcgtttc ttcctgataa atcaagcgtt taaccaggaa
     4321 tttgatatga accattaagc gatgataagt cagtgattct ttatcataaa cgtttgaata
     4381 ggatttttct agatgggaca aaatcgtggt cgtcatttct actattttta agctctttaa
     4441 actattaggc tctcctagct cagcattaat gatatgcata gtgatgaatc ctacttcatt
     4501 tgaccctaac tcggtttgat atcgatgatt gattcgctcg accacttcta ctgctatatc
     4561 atattcattt ggataaagga atctcatttc tggcaacata attgctgtga catctctatc
     4621 ttctcttttt cgttgaatag caaaataaat atgatctgtc aatgtaatat agatatttga
     4681 atctaatgga gaggagatcc tagctgttat cagttcaatc acttcttccg caaaatccat
     4741 aaattcgatt ggaatctgcg agactatctc actgaatcgt ttgatctttt ctttagaatc
     4801 taatacaaat aatttgtcag catcctcaac atcaaatggc ttactcactt tagcagaaaa
     4861 gccaatccct ttccccatcg caattttttc tattccatcg tcatcgatca atacagcatt
     4921 atgattcagc tttttcatca catacaccgt taattcacct cctctatatg ataaaacaaa
     4981 acaacttgca gactccttca tacatatttt tgtctccaaa caatttttaa agacaaaaaa
     5041 cgacctaaga aagaaccctt tcttaggtcg tgcctaattg aatagtagca ccctacataa
     5101 tttacaaaaa tagtttaaca cataataaaa gcgttatcaa tatctttttt actcgtttta
     5161 tgataatact tgtatcgaat tgatttggag gtaattacat aaaaacaaaa aaaatatagc
     5221 aactctctat aagaaagttg ctatattgat aattataccg gcggccgggg tcgaaccggc
     5281 acgtcctcaa cggacactgg attttgagtc cagcgcgtct gccaattccg ccacgccggc
     5341 aaatttaaaa ataataactg gggtagctgg attcgaacca acgcatgagg g
//
LOCUS       DAYYHX010000175         5370 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000175 DAYYHX010000000
VERSION     DAYYHX010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.176"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1081
                     /locus_tag="ACHA6Y_002363"
     CDS             <1..1081
                     /locus_tag="ACHA6Y_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988434.1"
                     /translation="FFKENDYLKNKEIIIPEFDSRNILLALVRYVNWEGYENKNPYVE
                     EACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDKKSAETKPLF
                     QLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVYKNFVLNDLS
                     LNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLSEKEQLLYGK
                     IVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVSESSVKQFFY
                     RTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGLETEKTKIYP
                     ISLKGANIHLYHIAQELHLLKKHIS"
     gene            1302..3065
                     /locus_tag="ACHA6Y_002364"
     CDS             1302..3065
                     /locus_tag="ACHA6Y_002364"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM8988435.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3149..3607
                     /locus_tag="ACHA6Y_002365"
     CDS             3149..3607
                     /locus_tag="ACHA6Y_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM8988436.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3716..4399
                     /locus_tag="ACHA6Y_002366"
     CDS             3716..4399
                     /locus_tag="ACHA6Y_002366"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8988437.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4546..5160
                     /locus_tag="ACHA6Y_002367"
     CDS             4546..5160
                     /locus_tag="ACHA6Y_002367"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM8988438.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5176..>5370
                     /locus_tag="ACHA6Y_002368"
     CDS             5176..>5370
                     /locus_tag="ACHA6Y_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988439.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
BASE COUNT         1908 a          844 c         1106 g         1512 t
ORIGIN      
        1 tttttttaaa gaaaatgatt atttgaaaaa taaagaaatc attatcccag aatttgactc
       61 gcggaacatc ttgctagctt tagtacgtta tgtcaattgg gaaggctatg aaaataaaaa
      121 tccgtatgta gaagaagctt gtaacgagtt gattgattat gtagagactc atttttttaa
      181 aagaagatat tcggaagatg aaaagtcctt tatcatacga ggaatagaga tcgcaatcgg
      241 aagaaaggca tttccccttg gactgaaaga agaagataag aaaagcgcag aaacgaagcc
      301 gttatttcaa ttagtctgtc aaggattgga agagcaaaaa ggaattctag acttacaaga
      361 agaagatgtc tattatattt tttctttgtt caacagtaga aattatacca atgagaacat
      421 ggagctgtta agaaaagatt tcgaagttgt ttataaaaat tttgttctca atgacctatc
      481 actgaatgaa ttgattttac agattattag tatcgctgaa atcgacgatg cagaaaattt
      541 tattttcaag caatcctttt tacaatttgt ccgtacgttg tgggcagatg gacaagtatt
      601 tttaccggaa aggatctatc ttctatcaga aaaagaacaa cttctgtacg gaaaaattgt
      661 agaaatattg gaagagtgga aaaagaaaaa tcaaatccaa ctaagatgga atgaaaattt
      721 cattcgaaaa ttcgtcaaaa gtctttcttt gatcaacagt gaacaagcgg ggagacagga
      781 aatagaaatt tttgttgttt cagaaagttc agtcaaacaa tttttttatc gaacccaatt
      841 tcatttgtat gcggaagaag gcatcgctga aataaatcct attttatttc gttcattaga
      901 agaattgcct gatgaatgtc tatgtgcgaa aaaaagagtg gttttgtgcg atgctgctag
      961 ctatcaaaat ggattagaaa ctgaaaagac aaagatctat ccaatctcat taaaaggtgc
     1021 aaacatccat ttgtatcata ttgctcagga acttcaccta ttaaaaaaac atatttcttg
     1081 atgagacctt aattaggaga ccttctacaa agctttttaa ttgtctgtag aaggttttta
     1141 tttgagtcct gattcgtgcg aagatctact tatataacaa gctttctctg aaattttttt
     1201 aaaatgctat tgacttagag tcaactctaa gtagtagaat agataaagtg taacaaatct
     1261 attttcatta aaagaagata gtacggagga gagaatgtat tatgagtaca gtattacatg
     1321 tattggatgc aatcaatagc cccgcagatg tcaaaaaatt atctgttgag gaaatgaaac
     1381 agttggcaga agagatgcgc caaaaaatct tgcaaaagga cagcgatgta ggtgggcacg
     1441 ttggtccgaa tctaggtgta gtagaattaa cgattgcgtt tcattatgta tttgattcac
     1501 caaaggataa aatcgtttgg gatgtttccc atcagtctta tggtcataag cttttaactg
     1561 gtcgaaaaaa tggttttgaa aaaggacatt accgtgaagt gagcggatat tccaaccaac
     1621 atgaaagtaa acatgactat tttacagtgg ggcacacatc tacatctatc agcttagcaa
     1681 caggtttagc taaagcgaga gatctgaaga aagaatcagg aaatgtggta gcatttatcg
     1741 gtgacggctc cctaagtggt ggcttagctt ttgaaggatt gaacaatggt ggtgttttac
     1801 aatccaatct aatcgtatta gtaaacgata atgagatgtc tattgatgag aatcatggca
     1861 gtttatataa aaacttagct gagcttcgtc aaaccaacgg taaaagcagc aataacctat
     1921 ttaaagcaat gggatttgat tatcgttacg tggataatgg aaacgatctg gaaacggtga
     1981 tccgtttatt tgaagaagta aaagatattg atcatccgat tgtacttcac gtccatacgg
     2041 aaaaaggtcg cggttatcag ccggcaatcg ataataaaat gaaatatcat tggcatgtac
     2101 catttgatct gtccaccggg gaggcaaagc aagtaacttc tggagaaaca tttaatatga
     2161 tcatcatgga ttatttggat aagaaaatgc aaacaagtac agctcctatt gccgcagtca
     2221 atgcagcgat accaggcgcc tttggactga aaacattcca agaaaaacat ccagatcatt
     2281 attttgacgt ggggatcgct gaacagcact ctatcacttt cgcttctgct ttggcttctc
     2341 agggagtacg tccagtcgtc ttccaatcaa gtactttctt gcaacgggca tatgatcaat
     2401 tatcacatga tttagcgatc aacgagaatc cagtgattat tttagtcggt ggcggaacga
     2461 tctcagctag tgaccgaaca caccaaggaa catttgacat tccttactta tcaagtatcc
     2521 cgaacatcaa atatcttgct ccagcaacaa aagaggatat gatctctatg ctagattggg
     2581 cgttcgaaca aacagcgcag cctgtagcaa tccgaattcc tacacatggt gtagaacatg
     2641 gtacagctat cgcaaatgat tatagtattc cagcctatca aacagtaaat aatggaggaa
     2701 aagtagcaat tttagcttta ggcggttttc ttgaattagg aaaagaaaca gtaaaagctt
     2761 ttagaaaaaa aggaatcgaa gcaacgttga tcaatccagg aacgatcaca gaaaatgacg
     2821 tagaaacatt agataaatta aaagaaacac acgaattagt catcacatta gaagatggta
     2881 gtttgtcagg aggatttggc gaaaatatca gtcgttatta tggtgattct tcaatgaacg
     2941 tattaaattt tggtgcagag aaagaattca cggatagcgt tcctgtaggg cagctttatg
     3001 aacgttatca tttaacacca aaacaaatcg ttgaagatag tctgcgtgtc ttgaatattc
     3061 aataattcat tatgctcacc atccagataa ttgtttatac tagtatctgg atggtttttt
     3121 cattataaaa agcaaagggg tggatgttat gtatcagcaa gtagaagaat atgtcaaaaa
     3181 gttccaagaa aaatatccta gttatcaatc agaaaaatta ggtttctttt catttgggga
     3241 tacagaaaaa atggcagacg agttgagcga actagttgta aaaaagataa agactggtac
     3301 atcaagtggt tacgatctgt ataaacaaga gaatgagcct ttaccaaaag ttggtcaatt
     3361 agacgtggtc ctgaatggaa aaaatgaacc agtttgtatc atcaaaaata taaatgtgac
     3421 catcttgcct tttagtgaag tcacgaaaga gcatgcttgg aaagaaggag aaggggatcg
     3481 gacattagct tattggcgga aagtccatgc tgagtttttt aaacatgctt ataaagaagc
     3541 gttgaacata gaatttacag aagagaagct ggtcgtatac gaagaatttg aagtaatttt
     3601 cttttagtaa gaaaggtctg ctgaaagaaa tagctcatta ttgtctcctg aatagaaaag
     3661 ggttacattt ttatatgaag ctgtattttt atataacatt actggaggag atattgtgga
     3721 tacactggaa caaatcaaag gcggagtcgt cgtttcttgt caagctttgg aaaacgagcc
     3781 gctacacagt tcttttatta tggggagaat ggctttggct gcaaaagaag gcggagcagt
     3841 tggtattcga gcaaatacga cagaagatat caatgaaatc aagcgaatcg ttgatttacc
     3901 ggtaataggg attatcaaaa gagattacga tgggtcagaa gtgtttatta ctgcgacaga
     3961 aaaagaagtg gaagaacttt tggagacgca agcagaaatc attgcactgg atgcaacttc
     4021 tcgtaaacgt ccgacagaaa aaacaactgc tgaacttgtc caaatgattc acgaacacgg
     4081 tcggttagct atggcagata tttcgacttt tgaggaagcg cttcaagcgc aagaagatgg
     4141 ttttgatctt ttgtctacga ctttagcagg ctatacagaa tattcacgaa agacgcagac
     4201 acctgatttt gatttattga atcaaatcat agaacaagtc gatatcccag taattatgga
     4261 aggacatact gactctcctg aacaagtaga gaaagcatat gaattaggtg ctttttcagt
     4321 cgtagttgga tctattatca cccgtccgca attgatcact aaaagatatg tcgaagccgc
     4381 aagtaaagca aataactaaa aaacacgcac ggcacagatt cttgacaaaa aagaaaaatt
     4441 gatagatagt ttaaagcaga ttctaagaag atgaaaaaag cattactttt cattttgtct
     4501 tcaagatctg ctttttaacg aattttatag aaagaaggcg aaaccatgtt tgaacggagt
     4561 ccttcagaat tgacagaacg tgaaaattac aagttgctga ttggttcagt catccctaga
     4621 ccagtagcag tagtaacgac taaatcagca aaagatatag tgaatatcgc acccttcagt
     4681 tacttcaata tcgtcagttc gaatcgaccc atcttatcct tggcgatcca gagaaaacaa
     4741 gatgcagtaa aagacacagc aaagaatatt cttgagacaa gagaagctgt tatccatatc
     4801 cttgatgagg acaacgtaga agaagtaaat aaaacagcag ccaatctgcc taatgaggaa
     4861 agcgaactgt acctgactga cttgacttta actgattcag taatcatttc agtaccagca
     4921 ttgacagaag caaaagttcg ttttgaaaca tccttatatg aacatgtcga gatacggaat
     4981 aaaaataaag taacagctga tttatttttg ctagaagtac agaaatatca tttaaatgaa
     5041 acagtctatg atgataagac aggaaggatc gatccaagaa aattgaatgc tgtaagccgt
     5101 ttggcaggaa atgattatgc agcaatagga gaaattttca cgattgcacg tcccgaataa
     5161 aaaggagcac tagttatgtt aaaaataagc tataaaccat cgacagattc aaaagaaatg
     5221 aaaaaagaat atgaaacagt gaatgatttt ctgcaagggc agtacttaga agttccgcca
     5281 ttgcaagacc attttgtcgt tactactgtt actttagatg gtaaggaaat agaaatgcca
     5341 gatcaaacga ttagcggatt gtttaattac
//
LOCUS       DAYYHX010000176         5282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000176 DAYYHX010000000
VERSION     DAYYHX010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.177"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHA6Y_002369"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHA6Y_002369"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM8988440.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACHA6Y_002370"
     CDS             complement(1297..2133)
                     /locus_tag="ACHA6Y_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8988441.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACHA6Y_002371"
     CDS             complement(2317..3534)
                     /locus_tag="ACHA6Y_002371"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8988442.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACHA6Y_002372"
     CDS             complement(3683..3844)
                     /locus_tag="ACHA6Y_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988443.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACHA6Y_002373"
     tRNA            3917..4000
                     /locus_tag="ACHA6Y_002373"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5282)
                     /locus_tag="ACHA6Y_002374"
                     /pseudo
     CDS             complement(4240..5282)
                     /locus_tag="ACHA6Y_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1642 a         1078 c          838 g         1724 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aa
//
LOCUS       DAYYHX010000177         5254 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000177 DAYYHX010000000
VERSION     DAYYHX010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.178"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..479)
                     /locus_tag="ACHA6Y_002375"
                     /pseudo
     CDS             complement(1..479)
                     /locus_tag="ACHA6Y_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            complement(485..1348)
                     /locus_tag="ACHA6Y_002376"
     CDS             complement(485..1348)
                     /locus_tag="ACHA6Y_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8988444.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(1362..2258)
                     /locus_tag="ACHA6Y_002377"
     CDS             complement(1362..2258)
                     /locus_tag="ACHA6Y_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8988445.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(2433..3722)
                     /locus_tag="ACHA6Y_002378"
     CDS             complement(2433..3722)
                     /locus_tag="ACHA6Y_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8988446.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(3694..5211)
                     /locus_tag="ACHA6Y_002379"
     CDS             complement(3694..5211)
                     /locus_tag="ACHA6Y_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM8988447.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         1711 a         1088 c          812 g         1643 t
ORIGIN      
        1 atatgatcag tacaaatcct aataacaata cgcaacaaag aatatttctc aaaaaatcaa
       61 aaaatacttt ccatgcactt tgttcaaaaa tttgtaaaaa tgtgatatct ttgttttcag
      121 aaatcacata tatccaggat aaaagaaaca tggaataaat aagtaaaact acatatccac
      181 taattgaaag tacgattgtc tgttcttcta ataattgatt aacaagaaaa taaataatga
      241 tcgttaacag actaaggaac aagttcttac caaaatgttg ctttattcct tgaaagaaaa
      301 aataaatgat atttcctgat ccctcacctt tccattgcgc cattacataa aaaaacagca
      361 catgtagcag gcacgatagt aataacaggc aaacacacga ttacccagag aatacctaat
      421 aagatgtaat cagaaaccac aatcaactgt ccataaagtt tagtctcaac tattttcatc
      481 ttcctcactc tttcattcca gacatagctg ttccttgaac aaaatatttc tgtgcaaaaa
      541 tataaaccac tagtaatggc agcattgaaa taaccgttcc agccattaca ggtccataaa
      601 aagaaatgtt ctgccctgta aataaagcta accctgctgg taaagttctc atttttgaac
      661 tacttgtcaa tatcaatgga taaagcaagt cattccaact attcatcagc gtgaaaatcc
      721 ctaatgataa aagagtaggt ttgctcaatg gcaacatgat tttcaaaaaa atattaaact
      781 cacttaatcc atcaatgcgt gctgcctctt ccaaagaatc tggtaaagta ataaaaaagg
      841 aacgcatcat gaagatacca aatgcagttg tcatacgagg aatgatcaaa cctgcataag
      901 tatcaagcat gccaagcaaa ttgacttcaa taaatagagg aatcatgaag acttggaatg
      961 ggatcatcat tgtcagcaaa acgaaataga acaaaactga ttttcctttg aaacgcatac
     1021 gagcaaatgc ataccccgca agagaatcaa agaatacaga agtgatcact gtccctccag
     1081 caaaaatcac tgtatttttg atgtaatcta ataaagggat cgttgtccaa accttaatat
     1141 agttatctag ggtatagcta tctgcaaata ttctaggcgg aaatgagata atgtcttttt
     1201 cgtgtttaaa agaagaaata atcagccaca aaaaaggaaa cgcaataacc aacaatataa
     1261 ctagcatact aatcgttcga atgatttttt taccactaac tgcttttcga ctattcacag
     1321 taactgtaat cgagctttct ggtgccatac aggtttccct cctatttcat ttgtttttct
     1381 ttccgtgaca tgtacacatt agtggagata gctatagttg caacaatgat aaacaaaacc
     1441 gaagacagtg ctgatgcata gcccaattca taaggtgctg taaatcctcg actgtaaata
     1501 tattgaacag ctgtttctgt cttaaattgt gggccaccag cggttgcaac atatacttgg
     1561 tcaaacactt gcatcgaacc aatcagattc gttaaaatac aaaaacctaa ggagggaacg
     1621 atctgaggta gcgtaatgtt aaaaaactgc tgtcgatttg ttgcaccatc catttgagca
     1681 gattcataca aagagggaga gattccctga atagcagtca agagaatcgt catagtaaca
     1741 ccaaaattct tccaaacagt catgatggct accgtcggca tagcaagggt tggatcgcga
     1801 aaaaattgag ggatcggcaa gccgacttgt gaaaacaagt aaggaatcgc ccctacattc
     1861 ggatcaagca acatagaaaa aataatcgca attgacgtca atgaacaaat aactggaatg
     1921 taaaatgtcg aacgacaaaa acgattgaat ctggtatttt tagataatgc tactgctgct
     1981 aataaaccaa cgataatttg tgttggcgtt tcgagtacgg tgaaatataa agaatgaatc
     2041 aacgaattaa ctgctcgttc atcactaaat atacgtgtga aattttcaaa acttacaaaa
     2101 tttcttgatg taaagaaaat attcaaatct gtaaaactaa tagcaaatgt tcctaaaagc
     2161 ggtatgacag taaataataa taaaactaaa aaagatggag ttaaaaacaa atacgcagca
     2221 cgatcaggac gattccaata tttggtgaat ccgttcatat agtccacctc atctttcatt
     2281 ttgataaaat atacaatctt ggtttaaacg aatctgaata agatatccga acaatcgctg
     2341 tgatttttgc aataacacct atctgcaaat actaggtcga tcattcagat atctgtatct
     2401 tgccaacagt ctatcctgta taaagttctg tactattttt gcagtaattg attgatggca
     2461 tcatctgcat cgttcatgag tgtctcagga tcttctcctg ctaataattt ttcaatcagt
     2521 ggattgatga tatcgttatt gatcgcagag atttgggtaa aacctggtaa atatggctct
     2581 gcatagtcca tttgtttcga taattcagat acaataggat cattttttac ttcttcatct
     2641 tttgctactg aatccaagta ggcaggaaaa ccattttcga tactccattt tttaccaatt
     2701 tcagttgtat tccaatattt caaaaactca tagacagctt cttttttatt attatctgta
     2761 cttgccggaa tggcatatcc tacgccatct aaaatagctg tttttttgtc tgaatcactc
     2821 ccttgtggta aggtagtcac attataatca atctcgtttg ctttcaatcc gttatttaac
     2881 catggcccat tgatttcaat ggctagttga ccagcattca ttaaattgtc catttcggcg
     2941 ccagaaatcg attctggacc agtcttgtct acatggatca ttttttggat cgcttttaac
     3001 gttttaaggt tctcaggaga cgcaaattca gacttagtat aatcatcgtt cactaattta
     3061 ccgccatttg ctaacatcca gttatataac ggtgcagttc catccttatt gaagacaaaa
     3121 cctgatacat ttttactact atcagttaat tttggtgcca tttctgtcaa ttcttcccaa
     3181 gtagtaggtg gttgttcagt atctaatcca gcttgtttaa acaaatcatt attccaatac
     3241 atatacatgc cttgtacctg catggggaca aaatactgtt taccttctac ttcacctaat
     3301 tcaatagccg tttctttaaa atcagattta tctactccat catattccca gaaatcgtct
     3361 aatggttgta ccgctccgtt ttctacatac tgtgcaaaat ctggataatt taatgcaata
     3421 aagtcaggtg cttttttaga agaaatcgct gtaggaagtt tttcattcaa attggcccaa
     3481 gtcatgatat ccatttcaat tttgatgttt ttatcatttg ttttattaaa ttcattaacg
     3541 atttcttgca atatttcacc atcagatgca gtaaacccat tccaaaaagt caaagtcact
     3601 tctttatcag aatcagattc tgctgaactt ccgccaccgc aagctcctaa aatacctgtt
     3661 gcagctacca acaaagagcc aattaaccat tttttacctt tcattttttg ttcctccttc
     3721 atctgataaa cgacatctga ccacattcca agatagcggt ttcaataaac aattgatttt
     3781 cccatcttca gcttttacat ttgttgaatg acttggtttt acttgttcac ttttaggaag
     3841 attagttgct tttgtatcaa atccactcat ttcagaaaaa tcaatcactc cctctaatga
     3901 taattcagac aagtgaacag ttagattcat ttcttcttct ccacggttaa ctgcaaatat
     3961 gacaagttct ttgtcattta ttacggctat actttctaaa tacggcactt cttcaaaatc
     4021 ttccgtcgca tagtttgctg aacgaatttg tgggcttaac acctctcctt gtccatacac
     4081 agcagcttgc ataaatggat aatagatcgt ctgtctccaa gcttctccgt ttgtttctgt
     4141 cctgattggt gcaataacat ttaccaattg agcaagacaa gcaatcttta ctctatctgc
     4201 atgtttcaat aaagtgatta atagacagcc taatagtaat gcatcttcaa aattatagtt
     4261 atcttctagt aaaggaggag caatctgcca aggcgctgct tgctcatctt gttccgctga
     4321 atgataccaa atattccatt catcaaagga caggttgatc tgcttcttac tatgtttctt
     4381 cgccttgact gtatcgcaca tcgctgtcac ttcagaaatg aaccgatcca tgtctaagga
     4441 acgtgcaaga taatgagaaa gatcattttt ttggttgcca taatattgat gcagagacaa
     4501 ataatctatt tgatcgtacg cttgatcaag aactgttaac tcccaatcac caaatgtagg
     4561 catttgccga ttcgaacttc cgcataaaac cacctcaatt gaatcatcta cgagtttcat
     4621 cgccttagcc gtttcctctg ctaaccgtcc atattcttcc gctgttttat gtccaatctg
     4681 ccaaggaccg tccatttcat tccctaaaca ccatgtctta attccaaaag ggctctcttg
     4741 accattcgaa cgccttaaat cactccaata agttccttta ggaaaattac aatattcgac
     4801 taaattacga gctgcatcta ctccacgagt acccagattg acagccatat tgatttcggc
     4861 ccccacatgc tgacaccagt tataaaattc atgaatccca acttcattcg tttcaattgt
     4921 ccgccaagct aaatccaacc ttctaggtct atttgctatg ggcccaatac tgtcttccca
     4981 acgaaatcct gacacaaaat ttccaccagg atatctaatc atgggaatgt tcaattcttt
     5041 taccaagtga atgacgtctt ggcgaaatcc acttttatta gctgatggat gatctggctg
     5101 ataaatccct tcatatactg ctcgtcccat atgttcaata aatgaactgt acaaccgcgg
     5161 atcaactttg cctacacgct tgacactact aatcaacatt gtggcttcca taactgacct
     5221 cctttaattt atacatgaat aatacacctc gttg
//
LOCUS       DAYYHX010000178         5135 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000178 DAYYHX010000000
VERSION     DAYYHX010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.179"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(383..1849)
                     /locus_tag="ACHA6Y_002380"
     CDS             complement(383..1849)
                     /locus_tag="ACHA6Y_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8988448.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(2140..4224)
                     /locus_tag="ACHA6Y_002381"
     CDS             complement(2140..4224)
                     /locus_tag="ACHA6Y_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8988449.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(4366..4488)
                     /locus_tag="ACHA6Y_002382"
     CDS             complement(4366..4488)
                     /locus_tag="ACHA6Y_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988450.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAGAMIEEKKKE"
     gene            complement(4749..5108)
                     /locus_tag="ACHA6Y_002383"
     CDS             complement(4749..5108)
                     /locus_tag="ACHA6Y_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM8988451.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
BASE COUNT         1549 a         1103 c          830 g         1653 t
ORIGIN      
        1 aatggtgttc aatcagctga ccttcagcat tagctcaaaa agcggcaaaa tatgaggagc
       61 tatttttccg tttgtttctc taatactcag gtcaaatcgc tgattttaca acctcttcct
      121 taaacggtgt tccaaagctt gcatctgcgg taataagccc agccaaacga aaaatatgag
      181 gggctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct
      241 gattcacggt gttccagaag tagcttcttc gaaaaacaag ccgaaacttc acaaaaattt
      301 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
      361 tcttgtaaca agtatttcta ttttaaggct gaaccactgt ttctggttca gtgatttctt
      421 tgtttttgtc tcgacgagca attgctggca agatcatacc taataagatc aagacgattg
      481 gtgtgatgat attcgtaaat aattggaacc accatgcttt aggatcagca gtataatcta
      541 cttttggcac cattcctagg atacatgcaa acgcagtgaa ggcaaagcac caagcaccaa
      601 agataaaacc gatttttgga tttttaacaa atttatattc agaattgaat ttcttataag
      661 ctttattcaa catcatatat gccaagaaca cccataggta acgcattggc atgacaactg
      721 agttcaagtt cgtcaaccat tttactaatt cattcatatc tttgatacca aaaatcggca
      781 acaaaataat gattcctact agtatacctg tcagaagata accattcacc aatgtacctt
      841 tacgattacg tttacgtagc cagtttggta caaattctgg atccgcatct gctaacaaga
      901 tttttagtgg tgcatcaatt gaaaaggcta atgcggagat ttgtccaatc atgtttgtca
      961 atgcgtaaat gatcatcaat gagttaccga taccatagta attacctaaa atttgaaaag
     1021 cactgtatgc cccatttgcc atcaagtctt taggaatatt atcactagca aacagcatac
     1081 ccattgcgac tgagcctaat acagcacaaa tccctaccat tgctgctaag aagatcatcc
     1141 ctttaggaaa ttcctttgca ggatttcttg tagagttgac ataaggggat attttttccg
     1201 cccctccaac tgcaaatact agcatcgaaa cggttgtgaa ataactgaaa tcaaatgttg
     1261 gaatatatgt tttcacttga ttcatatgtg gtgtggcaaa ttccatcgtt ggattgatga
     1321 acggtgccgt tacagctaaa agaataaata agatcgacat gacgaacatc gctgttccgg
     1381 ccagtccgcc aatcactttc aaagtagaaa gtccttttgt tgataaatat aaaaaggcaa
     1441 gaaatataat aagtgaaata actgctacca tttgtactga catcgtattt acgatatttc
     1501 catttccttg gactgcccaa ccaccagcaa tcagaattgc ttgaggcttt tgtgccaaat
     1561 aaggaatatg gacgacccag taagtccaag cggcatagta tgccagtcgt ttattagatg
     1621 tcgtattttc gacccatgaa ctgacgcctc ctttactatc cttgaaggct gatcctaatt
     1681 gtccaacgat caatgcgtat ggaataaaat aaatggccaa aatcaatagc caagaggtga
     1741 caactgatac tccttgctga gcatagttat taactacatt ccccatgccc caaaccatgt
     1801 taaatgcaat taacgcaaca gtaaaccagc gtaattgttt gttgttcatt cgttctctcc
     1861 tttatgataa aaatttgtta tccagatgta atcatattca atttttaaca aacttacaag
     1921 acagtgttat tttatgtgtg tattctttta tttaatacac atcgtttttt catatttttt
     1981 ccatcaaaaa aataagttat tttcttttat ttcattgacc ttattgaatt tcaagctatt
     2041 ttgaattttc tgcaaaatta aaaacattag ttattttata atcctatcta ttttctttac
     2101 atttttcatt aagaaaagga tataaaaaaa agaggacgtt cattcgtcct ctttttttac
     2161 atgcaagctt cgcatggcat tcaaaacagc taatactgtc accccaacat ctgcaaatac
     2221 tgcatctccc attgatgcaa aaccaaaagc acctaaggca agtaccaaaa tttttatccc
     2281 aatagcaaag acaatgtttt gtttgacgat tttcaatgtt ttctttgcta aatggattgc
     2341 ttctgcaatc ttcgatggtt gatcattcat gatgactaca tctgctgctt caattgcagc
     2401 atcgcttccc aatccaccca ttgcgattcc tacatctgct cttgctagca ccggtgcatc
     2461 gttgattcca tctccgacaa atgcggtctt tttattgttt ctttgtaaga tttcctctaa
     2521 ccgctgcact ttatcttcag gaagcaattc gctatatatc ttgtccacgc ctacttgctt
     2581 cccgatatga tcagcaattt ttttcgagtc gcccgtcaac atgactgtgt tcttcacacc
     2641 agcctgtttt aattgaacca atgcgtctac ggcatcttct ttcaaggtat ctgcaataac
     2701 aagataacca ctgtaacttt gatcgatcgc tagataaagc aatgtaccga tttcttgact
     2761 taaaggaaaa gagatttcaa atctttccat caatttgtga ttacctgcta atacttgttt
     2821 gccatcaact tcagctaaga tcccttcacc tgcaaattcc tggatattag ttgttggtaa
     2881 aagcggacct gcgtagcctt ctaaaacaga ttgagcaatc ggatgtgtag aaaactgttc
     2941 tgcactcgca atatattgca tgaatttctt tggatcgatt tttgtatcga tcgtctggat
     3001 cgaaaagtca cctttcgtta atgtgccggt tttatcgaat accactgtct cggtttgcgc
     3061 aagcaactcc aagtaatttg aaccttttac taatacaccg attttacttg cgcctccgat
     3121 accgccaaag aaactaagtg gcacggaaat cactaatgca catggacatg agatcactaa
     3181 aaatgtcaat gctcgataga tccattcaga aaacgccgct ccagaaacaa caagtggagg
     3241 taaaacagct aataaaacgg ccaatcctac aacgactggt gtgtaatagc gcgcgaattt
     3301 agtaatgaaa ttttcagctg gtgctttttt actgctggca ttttctacta attctaataa
     3361 tttgctgata gtagaatttt cataagattt ttctacttga actaaaaatg gtttattttt
     3421 attgataaat ccgcttaata ctgtttcacc aacatataca gatttaggga ctgattctcc
     3481 tgtaagtgct gacgtatcga ctaatgattc tccttcgatg atcgttccat ctaacggtac
     3541 acgttcacca ggcttcacaa gtacaaactg accgatctgc acgtcttcag ggttcacggt
     3601 catcaatcct tccgtcgtct gcacgtgagc tgtttctgga cggatcgcca tcaattcgcg
     3661 aatcgatttt cttgaatgac tgactgccaa tccttggaaa tactcgccga tttgataaaa
     3721 tagcatcact gctacggctt ctggatattc accgatcaac atagcgccaa tcgtagcaac
     3781 agacataaga aaattctcat caaagatttt tcctttgacg atattcatca ctgccttctt
     3841 cactacatca tatcctatca gcagataaat caataaatac cctatcaagc gaattggttc
     3901 attaaaatca aataatagca ataaagcgat acctgcaagg gaaaataaaa tccggaacag
     3961 cgtatttctc tctttttcat ttccatgttc agatactttt tccttatcta tttctgttac
     4021 tttgacatct ggttctaatt ccttcactac tttttttgtt tcttgttcaa tcgtagccaa
     4081 atgatcttcc tctgcgtcaa tcgttagttt tccagaagtg aagtttaccg ttgcttcctt
     4141 gactccattg atcttctgaa ctcctttttc gattttcatt gcacagttcg cacaatctaa
     4201 accttctaat ctgtaacttt tcattttaat gacctcctcg cctcaacata tgaacatctc
     4261 ttcataacta attataaatg aatggttatt catatgtcaa ggtatccgaa agaaaaaatg
     4321 aaaaacatct gcatcggaaa atttcttcca tacagatgtt ttggcttatt ctttcttttt
     4381 ctcttcgatc attgcccctg ctcttatcca acccgcactt gccttcttga tcttgacagg
     4441 atagcaacta agtttgaagc ctgttgctgg caatttgtct aaattcatta atttttcgat
     4501 aggacaatat cttttttcaa tccctgcaaa atgtccttcc cagatattcg aagctccact
     4561 acgctttcaa aaatatagaa agagttcttt aatgaaaatt atttcaactt tccataaaca
     4621 gtaatcccat ataaataaaa gacgtaaaag tcacatggga gaacttatac aggaaaacac
     4681 atagagtcag gtgaaataca tgtatcccac ctgactctat gcgttttcta acctttatta
     4741 ttctcttttt atacaatttc tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa
     4801 gattgcttgg caaaaggtat ccctatgatc gtgatacata gaaggatgcc gctgacgaca
     4861 tgtgcgagag caattggtag gcctgaaacg atcagccaga tgatattaac gatcaatgac
     4921 atgccagact ggtgataaat tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc
     4981 ttaaaacatt gcataccgat tgggatacct atgatcgtga tacaccaaag tatccccgct
     5041 gctaaccagc taagaccacc gattagtccg ccgaatataa accagatgac atttcctaaa
     5101 caactcattg tatttctcct tctattactg ttttt
//
LOCUS       DAYYHX010000179         5106 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000179 DAYYHX010000000
VERSION     DAYYHX010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.180"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            236..2230
                     /gene="uvrB"
                     /locus_tag="ACHA6Y_002384"
     CDS             236..2230
                     /gene="uvrB"
                     /locus_tag="ACHA6Y_002384"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM8988452.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            2240..5056
                     /gene="uvrA"
                     /locus_tag="ACHA6Y_002385"
     CDS             2240..5056
                     /gene="uvrA"
                     /locus_tag="ACHA6Y_002385"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM8988453.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
BASE COUNT         1688 a          866 c         1190 g         1362 t
ORIGIN      
        1 attttattat tataggcgaa aagtgctgaa tcagacaaga ctgtaagctc gaaaaaatga
       61 aaaaaatgcg agtttctaac attttatgtt ataaaacata acatgtataa taagataaaa
      121 aagtggcgaa agtatgttcg gtgtggtata attttttagt aaggaatgcc tttgctttcc
      181 ttttcttttt attggtcgta aaatgaaaag gaatgaatga aaaggaggac gaagaatgat
      241 tgagagagat acctcccgac actttgagct tgtctcgaaa taccaaccgg ctggtgatca
      301 gcctgaagcg attaaccaat tagtggatgg cgtagtcggc gggaaaaaag cacaaatctt
      361 attaggtgcg actggaacag ggaaaacata tacgatttcc aatcttattg agaaagtgaa
      421 taaaccaaca ctgattattg cccataacaa aacattggct ggtcagctgt acggggaatt
      481 caaggaattt ttccctaaca atgctgttga atattttgtt tcttattatg attattatca
      541 gcctgaagct tatgtgcctt caagcgatac gtatatcgaa aaagattcaa gtgtaaatga
      601 tgagatcgac aaattacgac attcggctac tagttcatta ttagaaagaa atgatgtgat
      661 cgtcattgca tccgtttcat gtatttttgg attaggctcg ccttttgagt accaaaaaca
      721 agtagtttcg attcgacagg gagcagaact agatcgcaat cagttgattc gtgacttagt
      781 cagtatccaa ttcgaaagaa atgatataga ttttcagcgc ggtcgttttc gagtgcgcgg
      841 tgatgtcgtg gagatttttc cggcttcaag agatgaacgt gccttacgtg tggagttttt
      901 tggagatgag gtcgaacgta tccgagaagt caatgcactg acaggtgaag ttttaggaga
      961 aacggaacac gtagcaatct tcccggcgac tcactttgtg acgaatgatg aacatatgga
     1021 gcatgccgta gcgaatatca aagcggaatt agaacagcgt ttaactgtct tgcgaaatga
     1081 aaacaaactt ctggaagctc aacgtttaga acagcgaacc aattacgata ttgaaatgat
     1141 gttagaaatg ggctacacgt cgggaattga aaattattct cggcatatgg acggacgtaa
     1201 agaaggtgaa ccgccttata cgctattgga tttctttcct gaagattttt tgatcgtggc
     1261 ggacgaatcg cacgtaacca tgcctcaaat cagaggaatg tataatggtg accgagcgag
     1321 aaaacagatg ctagtagatt acggtttccg tttgccatct gctttagaca atcggccact
     1381 tcggttagaa gagtttgaaa aacatgtcaa tcagatcatt tatgtctctg cgacgccagg
     1441 accatatgaa catgaacaaa cagataccgt tatccaacaa atcatccgtc cgacagggct
     1501 gcttgaccca gtgatcgaag ttcgtccaat catgggacaa atcgatgatc tagtaggcga
     1561 aataaatgaa cgagtcgaaa aagaccagcg ggtgtttgta acgacattga cgaaaaaaat
     1621 ggcagaagac ctcacagatt atttcaaaga attagggatc aaagtcaaat atttgcatag
     1681 tgatatcaaa acgctggaac gaacagaaat cattcgagat cttcgattag gtgaatttga
     1741 tgtgttagtt ggaatcaatt tgcttcgtga aggattagac gtacctgaag tttcccttgt
     1801 tgctatcttg gatgcggata aagaaggctt tttacgtagt gaacgttcct tagtccaaac
     1861 gattggacga gctgctcgga atgaagaagg taaagtaatc atgtacgcag acaaagtaac
     1921 agattctatg cgtctagcga tggatgaaac gtcaagacgt cgtacgattc agcagaagta
     1981 caatgaagaa catgggatcg ttccaaaaac gatcattaaa gaaatccgag atttgatttc
     2041 tattacgaaa gaatcggaag atgatacaaa agaagcggta caagtctcgt acgaagaaat
     2101 gacgaaggaa gaaaaagaca cgttattgat gaaattagaa aaagaaatga aagatgcggc
     2161 aaaagctttg gattttgaaa cagccgcgaa tgtgcgagat atgattttag aattaaaagc
     2221 ttcaaagtag gaggacaaca tggctaacga taaaattacg attcatggtg cacgtgccca
     2281 taacttgaaa aatatagatg taacgattcc acgagataaa ttcgtggtcg ttacaggttt
     2341 gtccggttct ggaaaaagtt ccctagcatt tgatacgctg tacgctgaag ggcagcggag
     2401 atacgtagag agcctttctg cttatgctcg tcagttttta ggacaaatgg ataagccgga
     2461 cgtagatagc atcgacggat tgagtccggc gatttctatt gatcagaaaa caacaagtaa
     2521 aaatcctcgg tcgacagtag gtactgtgac ggaaatcaat gactatttac gcttgttgtt
     2581 tgcacgtgta ggacatccga tttgtcccaa cgatcatatc gagattacca gtcaatcagt
     2641 agatcaaatg gcagatcaag taatggcttt gcctgagcgc acaaagatcc aagtgttggc
     2701 accgatcgtg gtaaagaaaa aaggacaaca taaaaaaata ttcgaacgaa tccaaaaaga
     2761 gggatacgtc cgcgttcgag tcgatggtga gatctatgat ttaagtgaag tgcctgagct
     2821 ggagaaaaac aaaaaacatg atatcgcgat cgtcatcgac cggatcattg tgaaagaagg
     2881 gattcgttct cgcctatttg attctttaga ggcggccctg cgattagcag acggctatgc
     2941 tctggttgac gtgatcggtg aagaagaaat gttgtttagc gaacactatg cctgtcctta
     3001 ttgcggattt actgttggcg aactagaacc aagactgttc tcattcaatg cgccttttgg
     3061 agcttgtcca gactgtgatg gtttaggtgt caaattagaa gtcgatcaag atttagtgat
     3121 tccagataga acgaagacac ttagtcaagg tgctatcgtt ccatggaatc caatcagctc
     3181 tcagtattat cctcaaatgt tagcgcaggc ttgtgaaagt ttcggtatcg atttagatac
     3241 accattcgaa gaattaccgg aagagcatca ggagatcatc ttgaatggga cagatgatct
     3301 tttccatttc cattatgaaa atgattttgg tggcgttcgc gatgtagaaa cgactttcga
     3361 aggggtattg aagaatatcg agcgtcgtta tcatgatacg aacagtgatt tcacgcgtga
     3421 gcaaatgcgt ctttatatga cagaattaac ttgtcagaca tgtaaaggtt atcgattgaa
     3481 tccccaagct ctatctgttc aaatcaatgg aacgaatatc gggcaaacaa atgagttgtc
     3541 tattcaaaaa gcagtagatt tcttctcaaa cttaacgtta tctgaacaag agaaagtaat
     3601 tgccaaaccg attttaaaag aagtaaatga cagactttca ttcttggaaa atgtgggact
     3661 agactatcta actttgagtc gcgcttccgg aacattatct gggggagaag cgcagcgaat
     3721 cagattggct acacaaattg gatcgaatct ttctggagtt ttgtatattt tagatgaacc
     3781 atcgatcgga ttgcatcaac gcgacaacga tcgactgatt ggctcattaa agaaaatgcg
     3841 tgatcttggc aacaccctcg ttgtagtcga acatgatgaa gatacgatgt gggcagcaga
     3901 ttatttgatt gacgtcggac ctggggccgg agaacaaggc ggacaaatcg tggctgcagg
     3961 cactcctgaa gaagtagcta atgatgaaaa ttctttgaca gggaaatatc tttcagggaa
     4021 aaaatcaatc cctgtaccaa aagaacgccg taaaggtaat gggaaagcaa tcaagatcac
     4081 tggtgcttca gaaaacaatc tgaaaaatat tagtgttgaa tttccattag gagaatttgt
     4141 agcagtcaca ggtgtgtctg gttctggaaa gagtacattg gtcaatagta tcttaaagaa
     4201 atcgttagcg caaaaattaa ataagaattc tgctaagcca ggtaaattca agacaatttc
     4261 cggctacgaa agtatcgaaa agatcatcga tatcgatcaa agcccaatcg gccggacgcc
     4321 gagaagtaat ccagcgactt atacaagtgt atttgatgat atccgtgggt tatttgctca
     4381 aacgaacgag gcaaaaatgc ggggttataa gaaagggcgt tttagtttca acgtaaaagg
     4441 cggtcgttgt gaagcttgtc gcggggatgg aattattaag atcgaaatgc actttttgcc
     4501 tgatgtctat gttccttgtg aagtatgtca tggtaaacga tataattctg aaacattaga
     4561 agtgcattac aaaggaaaaa gcattgctga tattttggaa atgacagtag aggatgctgt
     4621 agaattcttc aagcacattc caaagattca tcgcaaactg caaacgattg ttgatgttgg
     4681 cttaggttat gtgacgatgg ggcaaccagc aacgacattg tccggtggtg aggcacaacg
     4741 gatgaaactt gccagtgaat tgcacaaaat ctctaatgga aagaatttct atatactaga
     4801 tgaaccaacg acaggacttc atagcgatga catcgcccgc ttgttgcatg tattacaaag
     4861 attagtagat gctggtaaca cagttttagt gattgaacac aatctagatg taatcaaaac
     4921 agcagattat atcattgatt taggaccaga aggtggagaa ggtggaggaa cgatccttgc
     4981 gactggaaca ccagaagaaa tcattaacgt aaaagaaagt tatacaggtc actatttgaa
     5041 aaaaataatg gtataaagag gatagatagc tgagatagtt aaaaaaacat aaaaaaatga
     5101 attttt
//
LOCUS       DAYYHX010000180         5029 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000180 DAYYHX010000000
VERSION     DAYYHX010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.181"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..1168)
                     /locus_tag="ACHA6Y_002386"
     CDS             complement(join(42..940,940..1168))
                     /locus_tag="ACHA6Y_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8988454.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1247..1663)
                     /locus_tag="ACHA6Y_002387"
                     /pseudo
     CDS             complement(1247..1663)
                     /locus_tag="ACHA6Y_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(1848..2684)
                     /locus_tag="ACHA6Y_002388"
     CDS             complement(1848..2684)
                     /locus_tag="ACHA6Y_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM8988455.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(2695..3624)
                     /locus_tag="ACHA6Y_002389"
     CDS             complement(2695..3624)
                     /locus_tag="ACHA6Y_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM8988456.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            3816..4169
                     /locus_tag="ACHA6Y_002390"
     CDS             3816..4169
                     /locus_tag="ACHA6Y_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8988457.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(4243..4899)
                     /locus_tag="ACHA6Y_002391"
     CDS             complement(4243..4899)
                     /locus_tag="ACHA6Y_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8988458.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
BASE COUNT         1553 a          977 c          817 g         1682 t
ORIGIN      
        1 ggatttgagt caatatcttg gacacatttt agtagagacg gttaagccat tttttctttt
       61 gctaattctt caaattcctg tggggttaga tagtgaatcg agctatggat tcgatttcgg
      121 ttataaaatc cttcaatgta gctaaatagc gctcgatttg cttcttcaaa atctgagtag
      181 ctagtcgtgt agacttcttc ttttttcaat gaggcatgga aagattcgat tccagcatta
      241 tcataaggtg ttccttttct actataagaa tgccttattt tgttggtttt aagccattgt
      301 tccacttctc ttgctgtgta ttggctgccc aagtcagtgt gtagaatcat tccttctgga
      361 atgtgataat gtatttttgc tttattcaat gtttgaatga cacaatccac agtcattcgt
      421 tttgaaaatg tataactaat aatttttttc gtgtgtaaat ccatgattga agataagtaa
      481 caccaaccat ttttcttagt aggaatgtac gtaatgtcag ctgcccattt ctcacagatc
      541 gtttcagtag aaaagtcctg atttaagaga ttctcttttg aaatgatcgg tttattagac
      601 ctttgaggtc tatatttttt aactacaatc gaccttaaat ttaattgttt cattagcttt
      661 tggacatgtt taagagatac tgaaatccct tcttttaata aaacttgatg gatctttgtg
      721 gcaccatagc gttgtttatt cgtaaaaaaa attgttgaaa tcttcttttt caacttatta
      781 tttctgattt ctgtagctgt caacggttta ttcttataaa aataatagac acttctaggg
      841 atttctaata accgacataa caaagaaaca gtgtaatcct tacaccattt ttcaataaat
      901 tgaacaagcg aatttaggtt ttccgagaga atatggctgc ggcttttttt aagatttcaa
      961 gttcctcatt taaacgagca ttttcttttc tcagttcagc tacttctttt ccagtcagtc
     1021 ctgtggactg atcaggtaaa tataaattct tccatttgta aatcgttgct tcagataagc
     1081 catattcttt cgctaaccct cgaacagaac gtccagtttg attcagttca acaatcattt
     1141 gtttaaaatt ttcttcatat cgggtcataa aaattcatcc tctcgtagat aagtttaatt
     1201 tattcttgtc tctacgaaaa gctgtctaag atttgatact aacaccataa gtgaattctt
     1261 ttatcaaata accactaggc gtaatctttc gattaataac agttattgct ttattcggat
     1321 gagacgttag ataaacaatt ttacactttt cattcttttc tctaagcatt tcgcctagtt
     1381 cgataccgct ataataggcg ttcaaatcga tgtctaggaa aaaaatatct gaggaataga
     1441 tttcattatt tttaatgaat tcaacaaatt gtataacgtt caatccctga acgatctcaa
     1501 atttccaagg acttaataaa attctggatt cttccaaaat ttctactagc attttttcaa
     1561 aaaccaaact atcatccaaa ataaatatac gcgttaccat aaaaagtacc tccactcgat
     1621 attttactat caaaatcatt tcatacaact tttcaacata catgttagga ataaattgta
     1681 attctcttca caatcttttc caacaccttt gaaaccgata tcaaatactt tcaggatgct
     1741 gcttctaaaa taaatatcaa acaaatgaat acatcatgca aaaaaagcca gacaaccgac
     1801 gtccttgcgt gtcagctgtc tgagctatgt ttatttattt tgttctttta aaaagaacga
     1861 tacacatact gatttttcgg agcagagact ttctcagcgt ttgtaacaac tacagatggg
     1921 atttcacgct tgaatggttg ataatagtct gattggatgg ttccttctat tttataccat
     1981 tcatcatttt taaccgttac tccttccggc atatgcgtca acaaaccaaa gactcctgaa
     2041 tctgccacac aatggataat gccgaatcgg aacaagaaga tatctgtatt gctatcgttc
     2101 ggtgtttggt aaacaaatcc ttcatagctg atttttttgc caatgaattc gctagggtaa
     2161 ttataaatta gctccataac ttctaaatag ttttcatctg tgatcttgat agtttgctta
     2221 tccccatact tttctttcaa ctcattcatt tggtctaagt agtctgattt gttgaaataa
     2281 atactggtat ccggctttaa gtattgtgtc tgcatctccg gatcccctac tgattcttta
     2341 cttactggga aattaaatcc tttggcttct acgatcgtag tgtcgagact aactgttgga
     2401 aacaacgtcc ccacgattac tggcaaagct agcagtacat aagcgattcc acgttgataa
     2461 ggttttgaca acccatggtc atgatcgtgc tcgtgtgcct tttcttctgc ttttccgttt
     2521 ttgacccaca aatacagctg aactaaagcc aaaagagcag ataaaatcat tgaaagaata
     2581 gctaagtagc gataatggac attgatatat tgatcgagtt tgccagaaac ttgcaagtac
     2641 atcatcaatt cgaaataccc agataaaatc aaaaaccgca gcatattttc cctcctaaat
     2701 aaataatgtg aatccaataa cgctaagggc aatcagtccg atcagcgata aaataaagcg
     2761 tcctgtaaaa tatcgtttca tcatcagtag atttttgata tcgaccattg ggccaaagac
     2821 caaaaaggca gctacaggtg ctgtgccgaa cagacttaac aaagaagatc ctataaacgc
     2881 atctgcttct gagcaaagag acatcgtaaa ggccaataac agcatgatga taatggctaa
     2941 aatctttgtt gagccgagcg ttagcataat tctagttggt aaataagtct gcatcgcagc
     3001 tgcgattaat ccgccaatga tcaaataacg acctgtatca aaaaattcgt cgattgaatg
     3061 tgtcaacaca tgcccagttt gatggatcag ctgatttttt ggttcattct tctcactttg
     3121 ttcttcttca tggacatgat gatggtgatt ttctgctact ttagacgtga ttttttcttt
     3181 caatacagcg tctttttgaa aataggcaag ccagacacca atcacaaggg caacaaaaaa
     3241 gctgcctatc acccgccagc ttgccatctg ccaagaattg ccaaatgcga taaaagtgga
     3301 aaaaatcacg attggattga tgatcggagc agtcagcata aaagcaaacg ctgtgtgaac
     3361 tggtacgcct tttttaacaa attgatgaac gatcggtacg atgccgcatt cacatgaagg
     3421 aaagaaaaat cctaagatcg aaccagtaaa aatagagaag atacgatttt caggaagcca
     3481 tctcttcaca cgttctgggg tcaaaaatac ttgtaaggcg cctgagataa gacagcctaa
     3541 taatacgaaa ggtaatgctt caataacgat cgataagaaa atcgtactca tctgcaaaac
     3601 cgaatttgga ataaaagaaa acatgagttt cctccttttt atttctagtt cattcgcttt
     3661 gcaaatatac gcttttattc aaaaaaaaca agttgctctg taaattttta cacaactctt
     3721 attttttacc aaagcacttc aattgacttt ttctgttatt cccattaaat taaggtctca
     3781 tacatatcac ttctatcata aaagggagaa taaaaatgaa acaaaccact aacttgactg
     3841 aagttcaaga catactgcaa cagtcatccg ttgccgtcat ttatctttcg atgcctaatt
     3901 gcagtgtttg tcacgctgtt cgtccacgac tagaggaact tctcacacat tatgatattc
     3961 cggcacttca tttggatgca tttgaaacac cagaagtagc tagtaaattt gaagtgctga
     4021 ctgcgccagt tgtcctgatt tttcatcaag gaaaagaagt ctttcggcaa gcacggttca
     4081 tcgactttaa aaaaatccgt tatctactag atgaactgac agcagaagat gattcccttt
     4141 cttatgaaga aatcttccga acaaaataat cgttgtccaa acagaaaaaa gcgattgaac
     4201 aagtgaaagt tttgccttca tttgttcaat cgctttttat ttttattctg ctgtattttc
     4261 ggaatgcgca tttgcgtcag aatgcaagaa atagatcaag gtttgcaatt cattcgtcaa
     4321 atcaacattt tggatcaata catctgacgg agcattcaat cgagcagcgg taaagttcaa
     4381 gattcctttg attcctgctt ccgttaactg attgactact tcttgtgctc tttcagcggg
     4441 tatcgttaaa attgctactt cgatttgttg gatcttgatt tgttctgaca tatcttccat
     4501 tggataaacg gggataccgt ctacgatgcg cccaacgatt tcctcattca catcaaaagc
     4561 acaactgata cgtatgctat tactttgatg gaatttgtat tttagtaaag cacttcccaa
     4621 gttccctaca ccgatcagcg caacatttgt caattgatcg tcgtttaatg tctttgcaaa
     4681 aaagtgcata agatcttcta catcataacc atacccacgt tttcctaatt cgccaaaata
     4741 agaaaaatct cgacggatcg ttgcactatc tacttgaacg gcttcactta attcagttga
     4801 agacactttt ttctttccag cattatttaa aatccttaaa tagcgataat acaaaggcag
     4861 tcttttagct gtcgcctttg gaataatatg atctttcacg aaatgacctc cacgtattgt
     4921 ctaattacat ctttgatttc tccatcatag cagtatttat aggaaaaaat caattccatt
     4981 gctcagatat tgtgaaattt tattctttat tcatttattt atcatttct
//
LOCUS       DAYYHX010000181         4976 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000181 DAYYHX010000000
VERSION     DAYYHX010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.182"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..1265
                     /locus_tag="ACHA6Y_002392"
     CDS             45..1265
                     /locus_tag="ACHA6Y_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8988459.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1262..3982
                     /locus_tag="ACHA6Y_002393"
     CDS             1262..3982
                     /locus_tag="ACHA6Y_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8988460.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            3966..4910
                     /locus_tag="ACHA6Y_002394"
     CDS             3966..4910
                     /locus_tag="ACHA6Y_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8988461.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         1847 a          818 c         1001 g         1310 t
ORIGIN      
        1 cattgaaaat ggtatgataa aagaaaaaaa ggaggaaatt tttaatggtg aaatttatcc
       61 atgcggcaga tttacatatg gatcggtcat ttgaaggact gacaactctt gataaaactg
      121 tacaggaaaa actattgaag acgaatctga ccgttttatc aaatatcatt gaacaggcaa
      181 taataaataa cgttgatttt gttttacttg caggagataa ttttcatcaa aatcgtcctt
      241 cattaaaaat acagaaacat ttttcagaac agatgaaacg gttagaaaag aaccatatac
      301 ctgtatttat aatattcgga aatcatgatt tctatcaaaa agagcggtat tggttctcct
      361 ttcctaaaaa tgtccattta tttacatccg aaacggtaga gacaaaaaaa ataacgataa
      421 aaagtggaga aactgtctct ctcagtggat ttagttatcg tcaaccgtgg attcaaaaag
      481 acaaagtgat ggaatttcct tctagagaac tcacagatta ccatattggg ttgtaccacg
      541 gggaacctgg cgtctctcaa aaaggaaact atgctccttt ccaaccctca aagatgcaag
      601 aaaagggata cgattattgg gctttagggc atatccatgt gccaacggtc ttaaatgaaa
      661 agcaaacaat tgtttatcca ggtgcgcctc aaggtcatac taaaaaggaa caagcttcta
      721 catctattct actagttgaa ttaagtaaag gaagttgcca aatacatccg attaaagtag
      781 cagaggtcta ttggaagaca aaggaaatat cattgaggaa agcaagaacg acaaaagagg
      841 tcattgttca cattcgtcag cagctttcaa aagtagagga tggatttagt ttaattgaga
      901 taaaattaaa agattatgac catctcaata cagatgtttt ggaacggata caatcaggag
      961 agctattaga ctatttgtta gaggaattct cagataggat aaatgatttc tttatttggc
     1021 gtatttctct catagagaat actttttata cgaagacccc acttgctgct tctgctaaat
     1081 tgatggaaca gttatttcaa tactatcaga cacctcaaga tttccaacaa attttaaatg
     1141 aagtatatag tcatcaagaa gcggctagaa tattgagcga actgccggaa tatcaagaag
     1201 aaacacttga aaaagccaag cagctgctta atcaagattt tttatttgag gaggatcagg
     1261 aatgaagatt ttaaggatcg agattgctgc ttttggaaaa tggcgtcaaa aaagttttga
     1321 cttttattca ggcaatcagc tgatctatgg gggaaacgaa gcaggaaaat caacgattta
     1381 tcaatttatt caagcaatat tatttggttt cccttcaaaa ggccgtaaaa aaaaggatta
     1441 tacaccaaaa gacggctcag cctatggcgg caaaatatgg cttaaacatc ctgtatatgg
     1501 tgaatttgct gtcgagcgct acaagcagca aaatcgcgga aaatcgaagg tgtggctggg
     1561 ggatcaagtg ggaagtgatg agttactaga aaaactgatc gcacctttga cgaaagagtt
     1621 attccagcag gtatttactt tccagcaaga gcagttgatg gaactagatc atttgagaga
     1681 aaaagagcta catgatgcgc tgatttcttt aggcatcacg ggcagttctc aagtattcca
     1741 aaagcggcaa gaatattatc agaaagcaca acaattattc aagaaaaaag gccaaaaatt
     1801 actaatcaat caaaaattaa atgaatggaa agagctgcaa gaaaaaattc atcaaaaaca
     1861 agaacaggaa gcttattttg gcaagttgat ccaacaagaa caggaatacg agtcgcaaca
     1921 gcagcagtta ttggcacaat taaaagaggc gaacaaacaa ttttcattag ccagacagca
     1981 agaacttaat ttttctttat atgaagaatg gcaatctctc aaaaaaaaca gcactacagg
     2041 tcttcctgat gagcaacttc ttttagatct ggagtctttc tccaaacggt atcagcaaac
     2101 aaatgagcgt ttggaagaat tagaagcaac actggaaaag catagcggga tggatcagca
     2161 gtcagccaga tattattttt atttagagca ggagcaggaa ctaaaagatc tccagcaact
     2221 tcaatttaca gcggaacaat taaacgaaga atggcaaaga ttacaaattg agactagaga
     2281 aacaaattca gaattgaaga ttttggaaca aaggtggcat tggcagaaaa atccgttacc
     2341 ggaagaattt ttatatgaag aagaatggaa gcatcttctg gctgaagaaa gaaggattgc
     2401 tgatctagaa agccagattt ctgtgcgtct ccaaatagct aaagaacagc aagagatgat
     2461 tgaagcagaa gtgaatgact tggaagagac ctatccagca ttattacaaa atcagtcaga
     2521 gatagcacca gtctcttcat ctaaaccgta tatgctgatg gctggcggag caataatcat
     2581 tgcaggtata tttgtaccaa tgcccttgaa agtgatcctt ttactaattg gtgcaggcat
     2641 gattggctat ttcttctaca aaaggcaacc aaaaattaaa actaaaaatg atttctctga
     2701 gataaaagga atgtggcaag aaaaactagg acaacttgat ctagccttag ctcaattagc
     2761 tgaagcagaa gaaaaacaaa agagaactgg agaagaaaaa cagattcttc agcaagaagt
     2821 atatcgtttt gcggatcatc ggcacttagg aaagatggat acgctcaaac tcatgaaaga
     2881 atatggacag gaagtcatag attatcagaa attatatggc gtatttgagc ggaaacataa
     2941 aagagaacag gaaatacaag aaaaactacg acaaatcgac caaaaatttg agtttgttaa
     3001 agaatggtta ccgatacaag ataagacaat ttcagaaaaa atggtattgc tttcccgttt
     3061 tatacaagag atggaacaga tgaaatttgc cagaagctat cagcaaaata ctttattgaa
     3121 gcaagaaatc aatcaattga aaaagaaaca aaaagtatgg atacaagaat acagcgaaca
     3181 attgactcaa gcaggcttat cttatccttc ggaagtacct gtctttttac aaaaagccaa
     3241 acaagccgaa cagcagatga aaagatacaa tgaattatcg gaaatgctta ctccactatt
     3301 tcctgaagag ctgacaaaag agaaattatc tgtaagactg cgccaactga aagaaaagca
     3361 atcccaactc cagcaagaag aaaaagaagc aagtgaaaat cggcaacgac tccaacttca
     3421 agtagaagag ttgcaaagag acggcacatt ggacgaactt tatcaacaag ctagccggca
     3481 aaaagcaaaa ttacaggaat tactggagga atggggagca cttgaaatcg ccgggacatt
     3541 cttaggtgat ctttcaacag agatgagtga aagacaatta ccccagttgt taagagcagc
     3601 aggtgattat ttaggtattt taacaaaagg aaattatcag aagatattac tgaaaaatga
     3661 tacactgact ttaacagacg gatatacaga atttcccatc tatgagttat ctacaggtac
     3721 gaaagatcag ctgattatgg cgatgagatt tgcttacctc tcattagaag ctaaccgttc
     3781 gatatgcccg atcatcattg atgatggctg gcttcattat gatcatcagc gaaagtatca
     3841 attagctgaa cttttagcta aatttggaga aaagtatcaa ataatttgtt tttcttctga
     3901 taaagaaatg gtaagctatt accatgagat gaaccaaatc gtaaaacaat tagaaggagc
     3961 aaataatgaa aaaaattcgt gatttagtaa tcgatgagca gtttgaacaa tttgtcctga
     4021 tcaaaagtgc agatgtaaga atcgctaaaa acggaaaaaa atttattgct tttactttcc
     4081 aagatacttc aggtactatc gatggtaaat actgggatgc ttcggaggag gaaatcaata
     4141 aatttacagc cggcacagtc gtttttctaa acggtaaaag agaggtttat caaggaaacc
     4201 ctcaagtaaa gattcttcat atgcgcttaa caagggtaga tgagccagga gagcctagtt
     4261 tatacatgga aagagcccct ttgaagaaag aagaaatgga agaagaaatc aatcaattgt
     4321 tgtttgagat caccaatgcg cattggaatc gtattgttcg ctatctactt aataaatatc
     4381 aaaaacaatt ttttgagttt ccggcagcaa aaagaaacca tcatgcattt gccagtggat
     4441 tagcttatca tactttgaca atgcttcact tagggaaatc aatcgccgat gaatatcctg
     4501 agctgaatcg agcattgttg tatgctggta tcattttgca tgatttagga aaggtgattg
     4561 aactttccgg tcctatgtcg acagaatata cgcttgcagg aaatttgatc ggtcatctgg
     4621 tattgattga cgaagaaata actaaagcct gtcttgcgtt gaaaatcagc gaagctgacg
     4681 aagatgttct cgtattacgt catatggttt tagcacacca tggcttatta gaatatggct
     4741 cacctgtacg tccaagaata atggaagcag aaatattaca tcaaatcgac aatatcgatg
     4801 cttcgatgca gatgatgttg acttcgattc gtcaaacaga accaggaaaa tacaccgatc
     4861 ggattttcgg tatggacaac cgaagtttct atgtaccaaa agatttatag caaaaattta
     4921 tttttttcat tcaaaacaaa aaaagcgaga cataagtctt cgcttttttt gttttc
//
LOCUS       DAYYHX010000182         4883 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000182 DAYYHX010000000
VERSION     DAYYHX010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.183"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            509..2353
                     /locus_tag="ACHA6Y_002395"
     CDS             509..2353
                     /locus_tag="ACHA6Y_002395"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8988462.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2409..3875
                     /locus_tag="ACHA6Y_002396"
     CDS             2409..3875
                     /locus_tag="ACHA6Y_002396"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8988463.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3912..4754
                     /locus_tag="ACHA6Y_002397"
     CDS             3912..4754
                     /locus_tag="ACHA6Y_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8988464.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1605 a          785 c         1028 g         1465 t
ORIGIN      
        1 cgctaatcta attcactgta ttatcatttt ttagtagtat agcacaatta tgaatgcctt
       61 ttctttatat caaataaagt ttatataaaa aacctcatcc tgcaagaaag aaaaaatgaa
      121 cccattcttt ttatactagg tcttaaaaag acagaacatc tgctagaaga tagaagagaa
      181 taagagttat atatgttgga agatattctt agaaaaaaga caaaactttt agaacacgaa
      241 aactatattg aacgaataaa atgaaaaaat gattaaaaaa tcattttatt atttacgttt
      301 acattctaat atgttaaatt gaatataact taaacaggca tgtgactggt aatgcaggca
      361 ggacctaaat aagttgaata acaatgtatg cgtttcaata cacgtttgcc ttgttgtcaa
      421 tttatttagt ttctgcctgt tttttgtgtt gaggatggcc acttcgactc aaaagttcat
      481 cactcaaaaa agaaaagagg gaaacaccat gaattacaaa caaacagcag aagaagtatt
      541 gcagtacatc ggtggggaag aaaacatatc ccatttggag cattgctcaa ctcgtttacg
      601 ttttacgtta aaagacaaca gcaaggtaaa tatgagccag ttagaaaaag tggatggcgt
      661 tatgggcgtt cgccaaaatg tgcaatgcca agtaatcatt ggaaacgatg ttgttgaagt
      721 gtatgatgaa gtgaaagcgt tattaggtga caatgtttca aatactgaag aagcaccaaa
      781 acaaaaacaa aaattaggtt cagttctatt agattttatt atttctattt tccaaccttt
      841 aattcctgcg attgcaggtg gaggagtatt gaagtccttg ttgctactag cttctatctt
      901 agggctgatt agtgaccaat ctcaaaccta tcagatttta aatttggtcg gcggggcacc
      961 gctttatttc ttgcctattc tggtggcaat gactacggct aataaattga aagtcaatca
     1021 attggttgca gtatctgctg tcggtgcatt gatccttcct gaaatgacag ctatgttggc
     1081 ggaaggtgct acattcttat cttttggatt ggataatata gcgtatgcat ctcaagtatt
     1141 cccagccatc ttaactgttt tattttatgc tcaacttgaa aaatggttta caaaagtttc
     1201 accaaaacca attcgtattt tctttgtacc tatgatgagc ttattgatta cagttcctat
     1261 tgctttgttg cttctaggac cattaggtta taatgtagga acagctttct cttctatcat
     1321 tgtgtctttg tacaatcaat ttggatgggt tgcaaccgga gtcttagcag ctgtactgcc
     1381 attaatggtt gtaacgggta tgcataaagc aatgatccct tacgctgttt catccatgag
     1441 tgaattgggt gcagaacttc tttatctccc ggcttcattg gcacacaata tttcagaagc
     1501 tggcgcatgc tttgctgtca gtatcaaaac aaaagacaaa aaattgaaat ctacggctgt
     1561 ttctgcgggt atatctgcat tattcggaat tactgaacca gctctttacg gggtaacgat
     1621 tctgcacaaa cgtgttttat atgccgttat gttttctagc ctgatcgggg gagcatttgc
     1681 aggaatcgtt gcacttcaag cctttgcttt agtgggtcct gggattgcaa gtatcacgat
     1741 gttcgctgac aaagctgacc caatgaatct ggtttgggca tttgcagtat cggggatttc
     1801 atttgtggtt gcttttgctg cagcgttggt tctttataaa gataaagaag ttgtcaatga
     1861 agttgaaact aagatggaaa cggccgttgt ttcagatgct gttctttcaa gtcccgtgaa
     1921 tggaaaagta atctcactat cagaagtaaa agacactgtt ttttctagtg gtatggtagg
     1981 tgatggagta gcaatcattc ctgaagaagg gatattagta gctcctgaag atggagaaat
     2041 cacaatggta tttgaaacga agcatgctat tggtttaaaa acggctaatg gtgcagaact
     2101 tctttttcat attggtattg atactgttca gttagaagga aaagggtttg aggcatttgt
     2161 tcgttctggt gaaaaagtag ccaaaggaca aaagctgatt gcatttgata ttgatttaat
     2221 caaagaagca ggattcgacc ccacagttat ctgtgtggta accaataagg ataattacac
     2281 tatccgttca tttaacctag aaaaattggt aaccaatgat agtgatctct tagctatcag
     2341 tccgatagct taaacacaag atagtgaaaa aaaatatatt gaatgaagga atagaggaga
     2401 atagaataat gacaaacaca acaagttttc caaaaggatt tttatggggc ggcgcgatag
     2461 cagcaaatca agtagaaggt gcttggaatg tagacggtaa aggtctttca gtagcggata
     2521 tggctactta taaaccaaat ttagacaaca aagattttgc tgctcatatg catgtcaccc
     2581 tgcagtctat aaaagaagct agtgaagaca aatctgataa atggtaccca aaaagaaggg
     2641 gcgttgattt ttatcatcac tataaagaag atttagcttt atttgccgaa atgggcttta
     2701 aaaccttgcg cctctctatt gcttggactc gcttgttccc tacaggagaa gagctggaac
     2761 cgaatgaaaa aggggttgca ttttatgaaa acgtctttaa agaaatgcaa cgtttaggaa
     2821 ttgagccgat tgtgacacta tctcattatg aaatgccatt agctttagca gttaaatata
     2881 atggctgggt ggagcgtcga gtaattgatg attttgttcg cttcgctaac gtttgttttg
     2941 atcgcttcgg caaatacgta aaatattggc tgacatttaa tgagatagac agcatccacc
     3001 gtcatccatt tacaacagct ggaattatcc aagagaaaag cgcagaaggt caagaagctc
     3061 aagatatcta tcaagcattg catcatcaat ttgttgcgag tgcgttagtg acaaaagatg
     3121 ctcatgaaaa aattccagga agccaagttg gttgtatgtt aacgaaattg atgacttatc
     3181 cattaacttg cgcaccagaa gacgttgagc tgaccttgaa aaagaactta gaaaataatt
     3241 tttatgcaga tgttcaagtt aagggagaat atcctgctct aatcgaaaag gatttggaaa
     3301 atcggggaat tcagattaaa atggaagatg aagatcgaga aattttaaag aaacataccg
     3361 tagattttgt ttctttcagc tattacatgt ccatggcaga atcagctgat ccaaacgcag
     3421 aacgcacacc gggaaatact gttttagggg ttaagaatcc tcatttgcct tcaacagatt
     3481 ggggctggca aatcgatcca aaaggtttga aaatttcgtt gatcgaactt tatgatcgtt
     3541 accaagtacc gttgatgatt gtggaaaatg gtatgggagc agtcgatcag gtagtagatg
     3601 gtaaaatcca tgatccgtac cgcatcgact actttaaagc acacttccaa caaatgaaag
     3661 aagcaatcga tgaaggtgta gatttgatag cttatactag ctggggacca attgatttag
     3721 ttagtgcagg aacttctcaa atgtctaaac gatatggatt tatttatgtg gatgcagacg
     3781 atgaaggaaa tgggacatat aaccgcttga aaaaggattc gttcaactgg tacaaagaag
     3841 tgatcgaaac aaacggtgca tccttattcg agtagtctcg atctttttaa gcagcgatag
     3901 ggggataatg agtgatttca ataaaaaaag tattgaattc aagtgtggta cttgttgaag
     3961 aaaatggcaa agaaatgatt gcattaggta aagggatagg ctttggacag aaagctgggg
     4021 atgaaatatc aggagataaa gtagataaaa tatttctgcc ttttgaagaa aagaaatctt
     4081 cacaactatt agagttagtt gaagaaatac ctattgaatt ttttgaaatc actcgtacta
     4141 tcgtttttga agcagaaaaa ggattagata aaaaactgaa tcctggagtc tatttaacct
     4201 taacagacca cctgcatttt gcagttgaac gcgcacaaaa aggggtaaat attgcaaacc
     4261 gtttgtactg ggaaataaaa atctactatc ccaaagaatt tcaaattggg aagtacgcac
     4321 tcaccctttt gaacgagcaa tatggaattg atcttcctaa agaggaagcc tcaaacattg
     4381 ctttccattt gatcaatgca caatcggaag atcctgatag cggcgctgga ttcaagtatg
     4441 caaaaatgat cggcgggatc gtcaatttgg tccggtattc gttgcagaaa gatatcgata
     4501 ctaattcgat ccattatgcc cgcttcatta cacatgtacg tttctttgta gaaagatatt
     4561 attctgatgc tttgttggat gatggtgagg aagaattcta caatcaaatg tggatcttat
     4621 acccgtctgc catggaaagc gcagctaaga tcaaagacta cattgaaaaa gtttatcata
     4681 aaacaattcc gaacgatgaa gttgtatacc taggtgtcca catcaatcgg ttgatgaaac
     4741 atgctaatgg gtgagcgctc agtaatacta ttgatgcttt taaaatcttt agagatggat
     4801 tatagtaaaa acaaggtgat ttccagctgt ttgttatcta taagagacat ttaaaaagga
     4861 ttaacgtaag ataataaaaa atc
//
LOCUS       DAYYHX010000183         4817 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000183 DAYYHX010000000
VERSION     DAYYHX010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.184"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(365..1156)
                     /gene="msrA"
                     /locus_tag="ACHA6Y_002398"
     CDS             complement(365..1156)
                     /gene="msrA"
                     /locus_tag="ACHA6Y_002398"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8988465.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            complement(1347..1982)
                     /locus_tag="ACHA6Y_002399"
     CDS             complement(1347..1982)
                     /locus_tag="ACHA6Y_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8988466.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(2248..3300)
                     /locus_tag="ACHA6Y_002400"
     CDS             complement(2248..3300)
                     /locus_tag="ACHA6Y_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8988467.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(3313..4251)
                     /locus_tag="ACHA6Y_002401"
     CDS             complement(3313..4251)
                     /locus_tag="ACHA6Y_002401"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8988468.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         1563 a         1014 c          735 g         1505 t
ORIGIN      
        1 tagttattgt ttgcgctttt atacggaatt tagttcatca ttaatgtgga attcgattag
       61 gtgtctatta attatcaaga tgattctcta caaaattcca tagaaaaatc aagattctac
      121 aattcgaatt ctctttcaca tggaaataaa ggctagtaaa aaaacaggag ttgtgacaaa
      181 agacttgtca tacctcctgt ttccttgcga cttgaagctg aatgctttgc gccacaacct
      241 tttcctcggt gttctcaaag caactcctcg aaataattca taactgtcca aaaatatgaa
      301 aagctatttt tgtcagttgc tcattatttt gtcggagctg aacgctttgc gccacaacct
      361 tcctctatct atgtcgctga aaaaataaat gaagctgatg cgaatgcttg atctttttat
      421 atcttttagg ttgcttctga taaaattgtt gatgatgatt ttcagctggc caaaaagtct
      481 ttgcttggcg aatctctgta acgataggac ggctaaatcg gttagattga atcagttgtt
      541 ctcttgattt ttcagccaat tgcctttgct tttcattaac aacaaataga attgaacgat
      601 attgtttccc ccgatcttga aattgaccaa aggcatctgt agggtctgaa atttgccaat
      661 aaatctccaa cagctcttca taggttattt gacgtgtatc aaatatgatc tcaacagctt
      721 caacatgccc agtatatcca ccactgactt gatcgtaatt tggttttttg gtctctccac
      781 ctgtataacc agataacact gagattacac ctggttttgt ttcaaaaggc tcaaccatac
      841 accagaaaca gccaccggca aaaatcgccc gttcttggta taagggtgaa gttatcttat
      901 gtttggcata atcataagcc tttgcactcg ctgactcccc tgtgattctt aaataaaaat
      961 ccatcgcatc tggtgtcaaa ttatctcgta gtgctagcgg acgcaagttg acctctagtt
     1021 tttctatcac ctgctttatc gacatttttg ctaggtcttc ttttgcctct tcaaattgtg
     1081 ttctttccca ttctcttgta tttcgattta aaataatatt ataaaggtct atttccatct
     1141 gctccacatc taacatgaag tcaccctctt tcaagttttc ttcttattca ttcaaaaaat
     1201 gactacctga aatttagcac aacgcataac aagttaagac taacttgctt ttcgctctcc
     1261 tcatctctat tcttagtcga acacatccac acgaatggtt acccatagag aaattctttt
     1321 acttaatcag cttcacactg ctttttctac tttcgtagtt gaaaaaaact taaacaaatc
     1381 atcggcaaat agttaaagaa aaaacttaag cgttgaaaga agcccaattg cttaaagaag
     1441 gcaaactgac taatcgacgg aatccgtgct aatccgtaaa gacctgcaat tagtaaacta
     1501 ataactgtta aaacaagata aaaatggcta agcgtacctt gaccactttg acgataaagc
     1561 agaacaagta acaatgggaa aagtagaaag cctgcatagc ctagcccaga acctatatta
     1621 tggatccaag tcgatagatt ccaagagctt tcattcgtat tgatactgaa taacccagta
     1681 aaaatacaat cacctatccc atataaacca actgttgcaa cagcaagtat tgcaagtgtt
     1741 tttgatgttc caataaatag gtgatacaac gcaggcaacg aaagaacaaa aaaaagtcca
     1801 gatacaactg accagaccaa gaaagcacgt ctgactggac tatctacgtc accaaacaca
     1861 ctaattacgg tagtcatttg attcagtttg ggataaaaaa ggccaaggat atacggtgtc
     1921 gataaatcac taatcacacc taataataaa aaataaaaac catatttttg caaaaaaatc
     1981 attttcttct cctacttttt agtgattatt cgatacatcc aacaatcaaa ttttttctca
     2041 ttttaacaca tatttttatt ctagttgcct tttgaacaat caataacata aaggtgagtt
     2101 tcttctacat tttaaaaaat cgaatggcac tgaagagttc agttgtcagc atcaatctcc
     2161 tactcaaaaa ggcaaaaaaa cgtaagtcag atgttgttga catctcactt acgcatccat
     2221 tgattgaaaa tttcttagta aaatctacta gcctaaaaat ttgaagactt cttctcgatc
     2281 atacagttta aatactttct cgcataaaaa ttgagtaatt agtgccgcaa aaacagggat
     2341 gactaaccaa caaagaacaa caagaaagcc atttagccct gcatctaaag aagccaacgg
     2401 accaacaagt ccgaccacgc cgaaaccagc agaagcaggc gtacctgaaa tagaaaatag
     2461 agcgacagga atagctgtaa tcactgcact aactaaacaa ggaataagaa tcacaggttt
     2521 tctaaataga ttaggcatca tcatcttcat agcgcctaaa gcaatcgcaa ttgtcacacc
     2581 tgacttatta accttccaag agttcactac taaaacaaaa gtggttgcag ctacgcccat
     2641 cgcagccgca ccagcagaaa caccatctag ttggattgcc attccgattg ctactgttga
     2701 aataggagag ataatcaaaa aggcaaagga acaaccaatc aagatagaca ttaacagcgg
     2761 ttgtaaggta gtgaagttat taatcaatga tccaattgca gaagtaattg ctgttacaaa
     2821 agggaataaa taaaaaccaa gtaaacctgc acctacacct acaacaatcg gtgttaacac
     2881 aatcgacact gacccaaaac tatttccaat cagtaaaatc agcccgacag caatcgatgc
     2941 tgtcagcatc gtattaataa tatcgcctgt accagcacca acgtaaacac ccgtttcacc
     3001 caaatcaggt gtgaatttga ctacaccaga accaacaaaa gaagctccgg ctacaaccat
     3061 cattctcatc ggatcaaatc caaattgtaa ggcaatcaaa ccaccaatga tcaaaggtgt
     3121 tgctagttga aaaataactg ctaactgtgc gatcgttacc gcaaagggat acgcactaaa
     3181 atattttaaa atggcaccta atactgcatt aggaatcaat ccaataatga ttcctgttgc
     3241 tgtaccagcg aggactttat tcagaaaaat tttcggtgat attttttgtg aagtttccat
     3301 tcaatctctc ctttatttta cgttcaaact atcttctttt gcatggacaa gctcagtcaa
     3361 gaaatcacaa taaggcgtcg caacaccata tttcttccct tttctagaaa ttgcgccatt
     3421 gatataatcg atttctgtta atcgatggtt cttaatcaag tcttgataca ttgaaggata
     3481 atgtaaccca attgtttccg ggtcaaaaca agattcacaa tgtgcaatga cttctgggac
     3541 atctagttca atcttctcta ctgctgctac tttggcaaac tcattgacaa tcgtcgccac
     3601 cattttatga gcagtcgatg tttttcctag ctcagccata ttaacgtcta agattgtgca
     3661 tagtccgttc attgttccgt tcacacatgc tttgcgataa atagaatagt gaatattgtc
     3721 agaaaaatga gcatttaatc ctgattcaga caatttgtct gctaattttt ttgcagctgc
     3781 ttcttttcca tcacctaaat tttgtaattc tactgaacca cttccaaata atttgacctg
     3841 accaggacct tcaagacctg ccgtccacat ggtatttcca atatagatat tttccattgg
     3901 tacaaatttc tcaataatat cttcatgccc gataccattt aatagacata agacctctgt
     3961 atcttttttg attaatgatt ggatatcctg cagcattttt tccagctgca tcgctttggt
     4021 aaatagaata atcagatcaa cttgatcttc tttttctact tcggattgaa gaacgattgg
     4081 tagttttgct tctacctctt taccattaaa atttgcttgt aatccatgct ctttgatttg
     4141 ttggacatgt tctgcccagc catcgattaa taatacttcg ttcccacttt ggtgaagcat
     4201 taacccaaag cgacttccca ttgcgccagc gcctgcaatt gctattttca ttgattcttc
     4261 ctcctttttc tttctattat attcatttga gaatcatttc acaaccttgt tttgttattc
     4321 ttctcttatt tatcatgttt atatccacca ttcaagttat tttatcagca aatcaataaa
     4381 aactggaata gaatcccaat ttcatcgtaa gagaaactag actagcaata agaaaataga
     4441 aacattcata gaaaaaacct cactcctctg attataaaag agggggcatt aacctcgtgt
     4501 atactaaaac cttccattca ttcgatggat gattttcttt ttgtgaaaaa atattcattg
     4561 tagtacgatg ttgcgcaaca cagacggatg gtattgctta ttatttatta aaatcgaaca
     4621 atacacttat ttaaaaactt ctttcccaga ttttcttcca tttttacagt tataactagt
     4681 attatcatga aaaatagcct tttattgtct atgggaataa taatttaaaa ttggtacttt
     4741 tttattaatt agttcatcgc ccaataatct ctccagtttc ttagcatctc ctgactcaac
     4801 cggaattatc caagccc
//
LOCUS       DAYYHX010000184         4795 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000184 DAYYHX010000000
VERSION     DAYYHX010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.185"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..1889)
                     /gene="pepF"
                     /locus_tag="ACHA6Y_002402"
     CDS             complement(81..1889)
                     /gene="pepF"
                     /locus_tag="ACHA6Y_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM8988469.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2065..3063)
                     /locus_tag="ACHA6Y_002403"
     CDS             complement(2065..3063)
                     /locus_tag="ACHA6Y_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM8988470.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3289..3942)
                     /locus_tag="ACHA6Y_002404"
     CDS             complement(3289..3942)
                     /locus_tag="ACHA6Y_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM8988471.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="ACHA6Y_002405"
     CDS             complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="ACHA6Y_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM8988472.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1406 a         1007 c          744 g         1638 t
ORIGIN      
        1 aaaaaaatac gtcgactccc ttacaagaga gccgacgtat ttttttcgtt tctattcgag
       61 aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt cttctaattc atttagacgt
      121 tgttcaaaca tcgacattgc atcttcaata tatgcagcct gtgtcatatc aactcctgct
      181 tttttcatta cttccacagg ataatcgctg ttacctgctt ttaggtaagc caagtagttc
      241 tctagtgcct ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag
      301 ccagtcgagt attggaaaac atagtaatta taataaaaat gcggtatccg tgaccattcg
      361 aatttaattt ctgggtcttc ttcgactgct ggaccataat attttgcatt caatttacca
      421 taactatcac ttaggtattc actagtcaat ggcacacctt tttcatcttc cgtatgcatg
      481 aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag
      541 taatggttga gtacataagc ccgtacacga gggtcttttt ctgtttccaa taaatactcc
      601 gttaggatat tttcattcgt tgtcgaagca atttctgcta aaaagatgga gtagtcgccg
      661 tacacataag gctggttcga acgagtgaaa taactatgaa cactatgtcc catttcgtgg
      721 acaagcgtaa atagctgatc cagcgtatca tgccaattca ataaaatata tggatttgtg
      781 tcatagcttc ccgaagaata agcaccgctt cgtttccctt tattttcgac aacatcgatc
      841 caacgttcag agaatgcttt ttctacgatg gccatgtatt cttcacccat tggtttcagt
      901 gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg taattggagc ttcccctaag
      961 accggtgtat aaagatcata catgtgcagt ttttccactt ctaataaacg tttccgtaat
     1021 tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat
     1081 acactttcag gaatatgatt attgctcaaa gacgcttctc tggcagagct gtaattacgg
     1141 actttcgctt taaaattatg tccttttata tgtgtgccta aagtagaagc aaatgtattt
     1201 ctaaattgtt cgtaaacact gtacaacccc ttaaatgctg cttcgcgcac ccttcgatcc
     1261 gtgctttcta gcaactgacc atacacgcca tgagataatt ggactatttc accattttct
     1321 ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt atcacttgaa
     1381 gcatcaaaga tttcacctgc tccagcaaga agagattcct gctcagcaga taagacatgg
     1441 gctcgattat ctactatttg ctggatataa tggcgataga cttccaattt tggttcttct
     1501 ttgaaatatt gccaaatctg gtcatctgac aattgcaata tttctggttc aaaccaggaa
     1561 acggcttcac taactttcga aaacagactg cttgctcttg cataaagcgc ttggtaatct
     1621 gtatttccag tgtcttgatc gtttttaagg tgcgcatata cataaatgac ttcagtttgg
     1681 cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt
     1741 gttcctttgt gtttttcaga ttgttttaac tcttctgata attccaaata tttctcatca
     1801 aactcttggt cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc aggcaattct
     1861 tctcgttttg gcaactgctt tacttccatg aaatccactc cttttcttcc cttagctcta
     1921 tctatcaaaa tagatcatag tctaacatcc acatgtcaca gcatctaact tttctactca
     1981 ttcatgtgtc tgcttccatc aattttagca taactaaatc ttgttttaca tagaaaaccg
     2041 attcctagcc tatttttata cttactattt tttcaaagaa gacaagtgta tgcattcgtt
     2101 aaaaaactgc tgatagacca tctcttcatc aatcatagga aaaagccatt cccttttatg
     2161 ttcttttatt tggcaaagcc atgagttgaa atcaggaact ttctggtttt ccaaggcttc
     2221 ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt
     2281 ttgatagatc cacgatgata gatgcaaaag atgtttatga cgcaagtagc attgttcttg
     2341 tatggatact gtattctttt gcttgtgaat aagttggcgt gtcagccatt tcttcccatc
     2401 ttcactcact ttttctattt ttttcatttg gttcatttta atttcgttac tagtgaatat
     2461 ttctttaagg tccctactag aaaaagtcca gcagctactg caaaaggaaa tatgccctga
     2521 tacagtctct gtaatatgat gatacaaata aagcttttgt ttcttgagat cagctttcca
     2581 gcaatgcact cctctttttc tattatagta gcagcaactt ttttctatca atgagaattg
     2641 cgattgtcca agaaaagaat taccaagtat ccaccatact tggtaactgt tattcaaata
     2701 attttgggtt ctttcaataa atctctggtg agacagtcta gaacattgga tttctattgc
     2761 tagattcgcc aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa
     2821 tactggttct actgactgtc ccagccaatc catgaagcat tcctttaact gtaagtgctc
     2881 ttttgattct ctttcactaa agaccgaaca gttatctgat ttttgatggg caaaatgagc
     2941 acattttttc tttccctttt tcaaaatcac tctttttcga cagccacgac aataatattt
     3001 ctttcctttt tctgcatgtt tagcaaaaca gcacttatta tattcattca ttgcaagcag
     3061 catttttctc cctctgaact tatttacgtc taagaattta tttacacgaa aaaagagggc
     3121 gtgaaaaagc tattctgcat caagtaataa gaacagctaa acaaaaatag ttcccaatat
     3181 ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa
     3241 aaagactgcg acacaatttt tgtcacagtc tcttactata atgctgattt atacaaaata
     3301 aaatttcgtt cgctcgatag cattacgcgc catcagacat tgtccatatt cattcaatgt
     3361 ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct gattctctac
     3421 atccgcttgg ttgagatttt ccatccagaa gtgaacgact aggtaatagc gctcttcgaa
     3481 taaatacaaa tcagtccaag cagactctag ttcgacatct gttgcaagtt ggatcacttg
     3541 ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg cgcttccaga
     3601 taattcttcc tccggatcgc tgccaataat ctgctgacga agaaagtcac tcacatcttc
     3661 tgaatcaaca gtagaatctt ctgattgaga aatttcttca tccggaagat ttttactgat
     3721 aaacagttct aatccgtctc cttttggcaa aacttggaaa gtgacagctt cgcttcctcg
     3781 gaactcctct tcgatatcaa cttcttccaa aatactataa aagaaatttt cgatttcttt
     3841 gtgattgcca agtaaatcca aaaatgaaac gccccgttcc tctaaatctt catgaccaat
     3901 tagtacacgg atcgtatttt cattaatatg ttccatttcc attttgctac acctcacttt
     3961 gccacaatgt ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa
     4021 tctcgctgtt aagcgaattt aataaaacca gcggattttt caacctttca agctatctta
     4081 tttaactata acatttttgg cagacgaata ctatatagcc gacttttcag ctaattttcc
     4141 ctctctattt ttcgtgcaat catcatcttc tactaccttt ttatacatct aatcaagaat
     4201 actttttgta aaaacaaaaa aacttggaaa aactatcttt gtaatagtct tttccaagtt
     4261 tcgtattatc cttctataat ccagccatca attgtgcttg acgtaattct aattgtcgta
     4321 catcgcgcgg taggaaccga cgaatttcat cttcattaaa accaacttgc agacgtttat
     4381 catcaatcat gattggacgg cgaagcaatc caggattgtt ttgcacaagt tccaataatt
     4441 cttgtaacgg cagatcatct aaatccatgt tcagtttttg gaatactttg gaacgtgtcg
     4501 agatgatttc ttctgttccg tcttctgtca tttgtaaaat cgcttttaat tcagaactgt
     4561 tcaatggttc tgaaaaaata ttacgttcaa caaatggaat ctgatgttct tgcaaccacg
     4621 cacgagcctt tctgcaggaa gtacaacttg gggaagtata taatgttaac aacgtgcacc
     4681 actccttttc gtgataagaa tcactttttt gtaacagaag atcataataa tctcccatct
     4741 gtttctatta tacataatca acttttaaat agctaccttt ttttcataat ttttt
//
LOCUS       DAYYHX010000185         4779 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000185 DAYYHX010000000
VERSION     DAYYHX010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.186"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..171)
                     /locus_tag="ACHA6Y_002406"
     CDS             complement(22..171)
                     /locus_tag="ACHA6Y_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988473.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            1139..1465
                     /locus_tag="ACHA6Y_002407"
     CDS             1139..1465
                     /locus_tag="ACHA6Y_002407"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8988474.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1473..2381
                     /locus_tag="ACHA6Y_002408"
     CDS             1473..2381
                     /locus_tag="ACHA6Y_002408"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8988475.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            3007..3261
                     /locus_tag="ACHA6Y_002409"
     CDS             3007..3261
                     /locus_tag="ACHA6Y_002409"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFM8988476.1"
                     /translation="MIKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESL
                     DSFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            3803..3961
                     /locus_tag="ACHA6Y_002410"
     CDS             3803..3961
                     /locus_tag="ACHA6Y_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8988477.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            4033..>4779
                     /locus_tag="ACHA6Y_002411"
     CDS             4033..>4779
                     /locus_tag="ACHA6Y_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8988478.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDF"
BASE COUNT         1576 a          879 c          824 g         1500 t
ORIGIN      
        1 tacgttccag ttttattctt tctaaaaaat atactcactt cttttcttag acttcttttt
       61 ttgattctcc tctttatggt cttgtccttt tttgccttca aaatatttaa ataaacccct
      121 ttcaaaagca tttctaggtt ttggacaata aggaatgact tttttcttca tataaatacc
      181 tcatttaact atttgatatc atcacattat caaaaaaaat aggtttttta ttaaaactat
      241 cttgaccttt cgggagattt cactgtgatt tatttttatt cccatacttt tttcgttggt
      301 cactttcgta tacctaatct aagcgatcat cggcgacggt gcttttacaa cgaatggcag
      361 gacaaaacaa ggggcggaaa aacttttttg cctatttcat aaaaaacttt ttcctacagc
      421 aatcccttgt tttgtcctac cacacgttgt gaacccaaag cacgccgacg aacgcttagc
      481 tatacgaaaa tgacctccaa aaacaaaaaa gtgaggtgaa tagaaacaaa aagaaattga
      541 aatctcccaa aatgtcgtcc gcctcggaga caacttgctg ctgaaaatgg ttggttaatt
      601 catttaagta ttaaataaga taaatcatca aattaatcgg aaaaatagca tgattggcct
      661 tatcaaaaat aaactgtaaa aacttatatt tctatgagtg gtggaacaat gctaatacca
      721 aattaattta tcgattaccc acctgcacaa accaaagata gcattattag gaaacgaatc
      781 aatcagcgaa tccagcatta taatgaggat actaaaaata aagttttcat aattataaat
      841 ccgccttcct acgaaaggca ccaatgaggt gatgaaaacc ttagagcgct ttctttcttt
      901 ttttattccg tgttaccctt tccttgcagg tccctggtac actacagaac ccgaggaggt
      961 gagtggcggg gctgtatagc ggcaaccccg cataattata tggtgtcggt catccgttaa
     1021 ggcatcaatg aatggcgcaa acccgtagcc cgtaaactta tctcttccga agttgactcg
     1081 atttcgccgg atgaccagtc cctaccaaac ctgtactatt tttttacagg aggttgctat
     1141 gttcaaaatc tttcgtaaaa attgttgtgg cttggatgtc cacaaaacgt ggatctatgc
     1201 gtgtatcgga atcacagatg cgaatggtcg tacagtgtat aagcaagctc gcttctcttc
     1261 ctttacaaaa ggtctcaatg aattagctga atggcttgct aaatacaact gtaaggatgt
     1321 gtgtttggaa tcttccggta aattttggat tcctgttttt aatattcttg aaaaaacttg
     1381 ttttgtcaca ctggctcatc cgaagtacac caaaccccag aaaggaaata aaacagatcg
     1441 caaagatgca aaaatggatt tgtgatctct ttatgtgcga catgatcaaa cccagtttta
     1501 ttcctggtct tgaaatccgc cagcttcgtg atctaatgcg ttatcggacg aaactgacca
     1561 acatgaaaac aagtgaaaag aatcgtgctt taaactgctt aaccgtttct aatctgaaac
     1621 tagatgatgt ttttagtgat gtgttcggaa aatcatccac ttctatcatc caatacttgt
     1681 tagaacatcc cggtgaatca tttgatgttc gaccatttat ccatgcccgt tgtaaaactc
     1741 ccgttgaaga aatccaagcg gcagttgagg gggcgatttc tgtacctcaa gcgattaagc
     1801 ttcgccaatg cctcaaccat attaacgaga ttgagaagca tcgagaagag atcaatctag
     1861 agattcaagc tcttgcagaa cccttctccc ctgttttgga actacttcgt accataccgg
     1921 gcttagacac tcagccaata acagcgattg cgattctttc agaaatagga ccggatatgt
     1981 ccgtgtttcc aacttcgaaa cactttattt cttgggctgg atgctgtcca agaaacgatc
     2041 gcagtgcccg acgaacaaaa tcaacacgta tctctcgtgc agggcagtcg ttgaaaccgc
     2101 tacttgtaca gattgccaat gctcttctca gatccaaaga acatccagaa tttaaaaatc
     2161 gctatcgtag aatcaaagcg aatcgtggac acaagaaagc tatcattgct atttgtaaga
     2221 tgctcctgac cgctatctgg aatgtcttat caaaggtgga accctattct gctaaaggat
     2281 atctggagcc aaaacctgcg aaagaagaga aagttctgac gcaatcccaa gctctggacc
     2341 ttttaagaaa acgcggatat accattatag atagtgctta aaccaaagtt acgttgattc
     2401 ttcctaaata gactcgttgt cttccatttc aaaaattttg aaaacggtag gcttatttag
     2461 tgtaccgttc ttcaggtatc ttcaaataga tttattttca aacttatttc ctttcttctt
     2521 ttactctcct atagcttagc atttaagtgg gcactcgtgt caccttgcta gatagctatg
     2581 aatgccgtaa taagcctcct agtatatagg cagactgcca attataactg attcaatcca
     2641 cattatgata aaatccacct tttcgtttca tatgtgttga aatatcacat atgtcaaaag
     2701 tgcacgagtc ttttcattca gtgctttgta aatactcaaa attattcctt ttcactttaa
     2761 aaaaaagata attagtacaa aatcagaaat gaggaaagta ttaggatgga ctagaaagga
     2821 gttgttgaat ttgttgcaac agattggtca tttatcaatt aaaaattagg tgaagtttta
     2881 ttcatcactg cagctgctgt ttcccagaga ataaaacgtc tagaagaaac tggtcagtta
     2941 ctgtattatc aagcagcaat tgactacgaa caagcttgat tactaaccaa agcgtttatc
     3001 cacctgttga ttaaatcaaa tcaaaaatca ggtttttata cgttcataaa agcaatctcc
     3061 agtgtactcg aatgtgactg tatcataggt ccttattcga tgttaatcaa aatggggttt
     3121 ccttctaccg aatcattgga cagttttatc aatgatttat caaagtttgg acgaaccaac
     3181 acccaaatcg ttttctcgac tcttgtttcc ttaatggttt atcagttctg gaatgtttat
     3241 catgcgcttt tattaaaata gaaaagagct tttttcatgc gacaaagcag tgatcattta
     3301 ttggcacatg caatctttgt ggatccattt tttgaaaata cgatatttag ttttgaccca
     3361 aaaatcttta aatacgtatt tgataattta tcttaacaac cagataattt tattcagcac
     3421 cgtattatct atattgaatt ctcggaatca agataaattt ttttccgttt ttgtttcttt
     3481 tttaatcttc ttcttgttat taatagtgat taggatgata ttcctatatt gatctttgat
     3541 aattagatat aatttttgaa taacgttccc tgctattcga gctagaaata caagtacgcc
     3601 atgacctagt tagcgacaac tacttagaga tttcttttgt tctgggacaa caaaatgcca
     3661 tgacaatagc agtaaataat gatacttccg taaggctcgc cgtaaactgg ggagaggtga
     3721 aattcctatg tgcttaccca aagcggttca aaaattttaa actattattt taggagatga
     3781 ttttataaaa tcgaaagcct acttgaataa aaaacaagcc tgtgaatttc taggaattga
     3841 taataatact ttagataagt ggattcgtgt atataatttt ccagtaataa aaattgatgg
     3901 cgtagttcgc tttgacgtag aggatatcaa agatttcatg aatacattca aaaaaaattg
     3961 aaattttctt ctcgtcacat atactataaa tagcgaaatg tactgcctta ctttttaaag
     4021 gagtgaggaa gtttgcctac aaagctttca aatgggaaat ataaaactaa tctgaggtat
     4081 ccaaaaaaat tcagagaaat cactggaatt gcatcagaga agtatcaaaa agtatttcca
     4141 actaggcaat tagctattaa ggccgaaagg gaaatgaaaa agaagattga aacagtttta
     4201 agagaggaga atgcaaactc actagaattg aaaggaaaaa taaaatttaa agatttttat
     4261 gaatcaaaat ggcttcctag atatgaacta ggtcaaacta ctaggtcaaa cagggctcca
     4321 agtaatgtaa caattagtaa tactaaggat atattccgat tgcacatcct tccaatgttt
     4381 ggtgaatatg caatgaatta tctgaattca aatactgaga taatatcaga tgaattaaca
     4441 aagaaatcga aagaatacgc aaatataaaa attatcaaag gctatgttcg ctctatgttt
     4501 gatattgccg aaatactatg ttatatcgaa ttcaaccgaa ctaccaaaat tattcaatgt
     4561 attaccgcgc ctaagaaaat ggctttagaa gaaaaaagga taagggaagg gaatcaagca
     4621 ttatcatcag aagagctgac tgattggata gaagcagtaa acaatgactt aaataatcac
     4681 ttgcttacac ttcacgatta cacacttttc atgcttacct tatatctagg tgatagaaaa
     4741 agtgagactt atgctctgca atggaaatat attgatttt
//
LOCUS       DAYYHX010000186         4747 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000186 DAYYHX010000000
VERSION     DAYYHX010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.187"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..779
                     /locus_tag="ACHA6Y_002412"
     CDS             33..779
                     /locus_tag="ACHA6Y_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8988479.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            776..1615
                     /locus_tag="ACHA6Y_002413"
     CDS             776..1615
                     /locus_tag="ACHA6Y_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8988480.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            1616..2566
                     /locus_tag="ACHA6Y_002414"
     CDS             1616..2566
                     /locus_tag="ACHA6Y_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8988481.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            2707..3459
                     /locus_tag="ACHA6Y_002415"
     CDS             2707..3459
                     /locus_tag="ACHA6Y_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988482.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            3478..4629
                     /gene="queG"
                     /locus_tag="ACHA6Y_002416"
     CDS             3478..4629
                     /gene="queG"
                     /locus_tag="ACHA6Y_002416"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM8988483.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         1607 a          783 c         1025 g         1332 t
ORIGIN      
        1 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
       61 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
      121 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
      181 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagtcggtaa
      241 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
      301 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
      361 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
      421 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
      481 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
      541 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
      601 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
      661 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
      721 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
      781 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
      841 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
      901 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
      961 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
     1021 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
     1081 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
     1141 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
     1201 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
     1261 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
     1321 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
     1381 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
     1441 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
     1501 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
     1561 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
     1621 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
     1681 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
     1741 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
     1801 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
     1861 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
     1921 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
     1981 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
     2041 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
     2101 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
     2161 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
     2221 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
     2281 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
     2341 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
     2401 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
     2461 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
     2521 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
     2581 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
     2641 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
     2701 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
     2761 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
     2821 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
     2881 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
     2941 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
     3001 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
     3061 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
     3121 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
     3181 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
     3241 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
     3301 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
     3361 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
     3421 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
     3481 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
     3541 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
     3601 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
     3661 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
     3721 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
     3781 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
     3841 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
     3901 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
     3961 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
     4021 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
     4081 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
     4141 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
     4201 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
     4261 atggaaccaa aagtagacga aatatatcca aaattaaagc caatgctttc gatgtctaat
     4321 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
     4381 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
     4441 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
     4501 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
     4561 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
     4621 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
     4681 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
     4741 ttttgct
//
LOCUS       DAYYHX010000187         4703 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000187 DAYYHX010000000
VERSION     DAYYHX010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.188"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..190)
                     /locus_tag="ACHA6Y_002417"
     CDS             complement(<1..190)
                     /locus_tag="ACHA6Y_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988484.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(200..1015)
                     /locus_tag="ACHA6Y_002418"
     CDS             complement(200..1015)
                     /locus_tag="ACHA6Y_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8988485.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1277..1720)
                     /locus_tag="ACHA6Y_002419"
     CDS             complement(1277..1720)
                     /locus_tag="ACHA6Y_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFM8988486.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1854..2192)
                     /locus_tag="ACHA6Y_002420"
     CDS             complement(1854..2192)
                     /locus_tag="ACHA6Y_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8988487.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2180..2557)
                     /locus_tag="ACHA6Y_002421"
     CDS             complement(2180..2557)
                     /locus_tag="ACHA6Y_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8988488.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2781..3974
                     /locus_tag="ACHA6Y_002422"
     CDS             2781..3974
                     /locus_tag="ACHA6Y_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8988489.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4140..4562)
                     /locus_tag="ACHA6Y_002423"
     CDS             complement(4140..4562)
                     /locus_tag="ACHA6Y_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8988490.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         1373 a          897 c          823 g         1610 t
ORIGIN      
        1 tgcaacctgg accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta
       61 tcgacaaaag gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac
      121 ttgccaatga ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct
      181 gctctttcat aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat
      241 atttttgcat cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca
      301 tgattaatgc gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg
      361 ttttattttc cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa
      421 cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc
      481 gatagattcc ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga
      541 tactactgta gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca
      601 ccaacgacca caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca
      661 tataagactg ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc
      721 ctttaaaatt tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca
      781 acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag
      841 accaaataac cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg
      901 tgaacacttc attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg
      961 tcaataaagc taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt
     1021 ttgaacgaat ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat
     1081 ttctttgtaa tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt
     1141 cttatgtttg taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta
     1201 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     1261 cgattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     1321 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     1381 ggtcaagcgg aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta
     1441 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     1501 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     1561 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     1621 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     1681 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     1741 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     1801 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt
     1861 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     1921 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     1981 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct
     2041 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     2101 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     2161 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     2221 catatttctt gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt
     2281 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     2341 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     2401 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     2461 ttgtccgatt aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg
     2521 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     2581 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     2641 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     2701 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     2761 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg
     2821 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     2881 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     2941 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     3001 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     3061 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
     3121 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     3181 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     3241 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     3301 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     3361 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     3421 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
     3481 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     3541 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     3601 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     3661 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
     3721 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     3781 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     3841 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     3901 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     3961 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     4021 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     4081 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     4141 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     4201 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     4261 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     4321 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     4381 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     4441 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     4501 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     4561 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa
     4621 atatcatatt gtcacaccgt caatagtgca taaacctgtt attatttctc tttttttcct
     4681 aagtattcta ttgatatgaa tca
//
LOCUS       DAYYHX010000188         4653 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000188 DAYYHX010000000
VERSION     DAYYHX010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.189"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ACHA6Y_002424"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ACHA6Y_002424"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8988491.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="ACHA6Y_002425"
     CDS             2714..2938
                     /locus_tag="ACHA6Y_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988492.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            2951..3451
                     /locus_tag="ACHA6Y_002426"
     CDS             2951..3451
                     /locus_tag="ACHA6Y_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8988493.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            3548..>4653
                     /locus_tag="ACHA6Y_002427"
     CDS             3548..>4653
                     /locus_tag="ACHA6Y_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8988494.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIV"
BASE COUNT         1670 a          690 c          994 g         1299 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattggtttg tttttaggat ttaaacgcga
     2821 gttttccaaa ctgattggct tcttggtgat tgctttgatt gcggtaggtc tggtctttaa
     2881 tgcttctggt gtgaaggatg tcttactcaa tttgtttaac cgaattgtcg gtgcctgagg
     2941 gggaatcaca atggaacaga tacgtgttta tattgcgaac cttggaaaat acaatgaagg
     3001 tgagttggtg ggggcatggt ttaccccacc cgtcgatttt gatgaagtga aagaacggat
     3061 tggattaaat gacgaatatg aagagtacgc aattcatgat tatgaactgc cttttgagat
     3121 tgatgagtat acaccaattg aggaaattaa tcggttgtgt ggtttagcgg aagagcttaa
     3181 aggaacgcca attggtgagg tcgcttcaga gattcaacat gccttcttca attctttcga
     3241 ggaaatggta gagcatgtgg atgatatcat ctattatccc gattgtaatg acatgagtga
     3301 tgtagcgtat tacttgattg tagaagctgg tgatttaggc gaagtaccag aacacttgaa
     3361 gacgtatatt gattttgatg cgtgtggtcg agacttggag attggcggga attatttggt
     3421 tacgagtcga ggggtttttg aactaatgta aatcatttgt tagaattgac cagaaatttc
     3481 tatcgtactt ttacttttct atttgatata ctgaaataga aaatatttgt tgataggggt
     3541 gttactaatg gttggcaata agtggtacaa atgtgatctt catattcatt caactgcgag
     3601 tcagtgcttt caggataaaa aggttactgc taaggaatgg gtagaagaat gtttaaacaa
     3661 aggactcgat tgtgttgcat taactgatca caatacaggg gaaaatattg atgaatataa
     3721 gaaagtggct atcgaaaatg gcttagattt ttttcctgga gtggaattaa cgtgtggaga
     3781 aaatggaact catttactag tcctatttga taaaagtgca tgtacaaatg atatctctga
     3841 tttcttaacc aagatgggta taaaaagaga ggattgggcc agcgctgcag cttgctcaat
     3901 gaaatcagtt ttagaagtta ttgattttgc tgagcacgag ggcttgctcg ttattcctgc
     3961 acatatagat gagtttaatg gaatttcttc agcaaaattt ggaaatctag gaaagatatt
     4021 tgaatcagaa aatatatatg cagtacagac ggttcaaaaa gaattttttg aaaatcgctc
     4081 acaaatgatt ccctcatcta agcgtgacac tatctatgat agtgtgaatg ccagatatga
     4141 tggtagagtt ggaaaagata ctttggaatc ttggtataaa tcagttgttg aggcagaaaa
     4201 aaataatatg acatttctta gcttttctga taacccacac tcgaaaggaa attcaaaaca
     4261 tggactttgg ggaataggca caagatattc ctatataaaa atgaaggacg aacctgactt
     4321 gggatcactt cgcgatgctt taatgctagg cgaaactcga gttcattctg atttttctaa
     4381 tttctccatt aatgaaaatg aagttttatt ggaaaaatta agcttttctg ggaccactct
     4441 tagcacaaaa gaagttgtag tcgaattcag cgataattta acttcaatta ttggaggaag
     4501 aggaacaggc aaatcttgta ttacaagatt cttggtctat gtccttggaa aggaagcaga
     4561 acttgatcaa ttttctgaaa tacaaagtga ttatcagaac tttgcacaaa ttgaacataa
     4621 tggctctgga atatttttga aggatacaat tgt
//
LOCUS       DAYYHX010000189         4548 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000189 DAYYHX010000000
VERSION     DAYYHX010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.190"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            436..1152
                     /locus_tag="ACHA6Y_002428"
     CDS             436..1152
                     /locus_tag="ACHA6Y_002428"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8988495.1"
                     /translation="MKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIHEN
                     APFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHRLP
                     PEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHIPN
                     IEERGILEKNKLSKILSLAKAKTLARLLMFLLKSLVLHKYSQNILNYYFLEKSKKNRI
                     PNFHLHFQKDLHVDNQFLNQ"
     gene            complement(1118..1204)
                     /locus_tag="ACHA6Y_002429"
                     /pseudo
     CDS             complement(1118..1204)
                     /locus_tag="ACHA6Y_002429"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(1248..1709)
                     /locus_tag="ACHA6Y_002430"
     CDS             complement(1248..1709)
                     /locus_tag="ACHA6Y_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermophilic serine proteinase"
                     /protein_id="HFM8988496.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNR"
     gene            complement(1969..2517)
                     /locus_tag="ACHA6Y_002431"
     CDS             complement(1969..2517)
                     /locus_tag="ACHA6Y_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988497.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(2518..3375)
                     /locus_tag="ACHA6Y_002432"
     CDS             complement(2518..3375)
                     /locus_tag="ACHA6Y_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8988498.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYVEVE"
     gene            complement(3661..3948)
                     /locus_tag="ACHA6Y_002433"
     CDS             complement(3661..3948)
                     /locus_tag="ACHA6Y_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8988499.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(3938..4267)
                     /locus_tag="ACHA6Y_002434"
     CDS             complement(3938..4267)
                     /locus_tag="ACHA6Y_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8988500.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(4344..4490)
                     /locus_tag="ACHA6Y_002435"
                     /pseudo
     CDS             complement(4344..4490)
                     /locus_tag="ACHA6Y_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
BASE COUNT         1379 a          877 c          658 g         1634 t
ORIGIN      
        1 aagaggacag catatcgata ttgcttcctt ttttttgcta tttatttaag ggatagaagt
       61 ttagaaagga ctgtttataa gatgaaaaat gatatagtga cttatgcaaa cacaaaatgt
      121 gaagaaattg gcatcagtaa tttagaagag tgcttttttg acacaaatga cctgtcgcaa
      181 tttttaagaa cgggaagaac aaatgccagt aaacttgcca atgacttagt tgcaaagaac
      241 gttctattta aaatcaatac tcgtccagtg ttatttgcca tcaaagagcg ctttgaagag
      301 cactttttga ttccggtaaa agatgtatac gaaagtatat ctgaattaaa aacagacttg
      361 gagcccaatt ctacagattc cattctacaa aagatgattg ggtatgatat gagcttatcc
      421 gatgcggtgg aacaaatgaa aactgctgtc ttctatcctg gaagagggct tcctatgatg
      481 atcactggag atactggagt tggaaaaagt ttttttgttc atactgctta tcagtttatg
      541 aaaaaagtga acgttattca tgagaatgct ccttttaaag tacttaattg tgcgcaatac
      601 cataataatc ctgaattatt gtcggggata ctatttggct atgttaaagg tgcttttacc
      661 ggcgcagatg aagatcgaaa tggattgttg caagatgcag acggtggagt actgttttta
      721 gacgaagtac acagattgcc tcctgaaggg caagagaaac tatttgttta tatggatggc
      781 ggaacttatt ctagagtcgg tgaaactatc catcacgcct ccaatgtacg tttagttttt
      841 gcgactacag aaggcaaaga ggattttctg gaaaccttta tgcgaagaat ccctatacat
      901 atccatatcc caaatattga ggaacgaggt attttagaaa aaaacaaatt atcgaaaata
      961 ctctctttag cgaaagcaaa aactttagca aggctattga tgtttctcct aaagtcacta
     1021 gtgttgcata aatatagtca gaatattctg aattattatt ttcttgaaaa aagtaaaaaa
     1081 aatcgtatcc caaactttca tctacatttc cagaaagatc ttcatgttga taatcaattc
     1141 ctgaatcaat gaccgctacc ttcacatcct tagaaccctt ggtttcttcc caagcggatg
     1201 gcatgctaat ctctcttaaa ttagcttgtt tactccaatt aggatcgtta tcgatttcct
     1261 aaagaatcag ttgtatttgg ctcctcaaca tcattcaacg gctctagttc aaccacatag
     1321 tttggctctg cataattgac catatcagaa gcattaaatg ttcttactgc ctcttccaca
     1381 ctaatttctt cgttcaattt cactaataat acttgatctc gcttattata atcgctttca
     1441 tttgtcctaa gagtcaaatc tttttccacc ataactttta cacccaataa attttgcggt
     1501 ttatttttct ctgttgaaat tctttgtgta gcctttaaac tgacaattag ctcattgggt
     1561 acatactcta tttgagagtc atctaattgg ctcataatcc tttctttttg gttagtatct
     1621 gcagttactt gtttagtatg agccatgaac ccaaacatac tcaccccaac aaccaatcct
     1681 aaatatattt tctttttaaa ctttttcatc tacatctcct cctaatagtt ttattttaga
     1741 gttagctcta ttacttattt actaactcaa caacagatta tcatacactt attaatgaaa
     1801 tcacaatatc tcattaaaac acaccctaaa atcataaccg ttcctatgct cattttttgg
     1861 attttttaat cacgtaacca gaaaaataaa tcccctctat ttcacgcaaa tcaaagaaga
     1921 actaagtaac taccgtttaa aggtagttac ttagttcttc tttgattatt atctaacttt
     1981 attattcatt ccccaccctt tgcgcagtga gtcttcggta tcttgtttta tctgactggt
     2041 agttcttatt atttctccac gatcatctaa ttctggaaca attggatatt tttctctgaa
     2101 caagtctagt tctttcacca cgtcattaaa caaatcttgt ttatcttctt gcgtaagatt
     2161 ctgtttaata tactcatcta aagcttttct aagcaattca tctcttgtca tattagtctc
     2221 atacttttct actaaaactc tggaagtcgc atttaacaaa gtcgacagtt ttaaaggatg
     2281 cttggtggaa taaaccttat caaactttct gattggtgcg ccaacttttt ttgttcttcc
     2341 tctactctgt ctttcaacat tggaggaagt ttttatttct tgaacttggt gcacttctaa
     2401 ttgcttattt tcagatggtg taattgcttg ctgctttttc tttgcttctg cttctttttt
     2461 tattttggcg agtcctttgt tgttcccaaa aatatcaagt cctttgatgt cagccaatta
     2521 ctccacctcc acataccaat tctcgtgttc ttccaattct ttcaaaatac tgataaataa
     2581 ttcgtggctt ttgcgatccc attgttttaa gagacctttc tttgtcttct ccatcgtaat
     2641 acctgatccg tcgtatcgtt ttaagcgcgc atttttgagt acaacatttt taatcaaatg
     2701 ttccccataa atctcttggg cttgtttata catttcctga tctactgcgt caccgctttc
     2761 caacatgaaa ggaataacac cagcaacata taaatttgta tcaaattctt tcacaaaaaa
     2821 gtccatatac tcaagatatt gcatcgcatt tctcatcgat tttacttggg tctgtaagac
     2881 gacgattgcc caatccgcaa tgtacatcgc ggatttacta tatacagaaa tagaaggtgg
     2941 gacatctaaa tagatgacgt caaactcatc aaataatggt tctagctttt ctttcatata
     3001 tttgaattgg gataatgaat cattatattg gtctttgatc caatcatcat acatttcaaa
     3061 ggacatgtct gctggaagta tatagaggtt attgattact ttatagacag cctctttagg
     3121 tggaagattg tttatcaaca cgtcagtcac agtattttcg taaacaatcg gttcaccttc
     3181 tccgcctcct tcttcgttag tgaagttcat atgcgttaag ttcatcacat cagaagtatt
     3241 tccttgcata tccaagtcaa ttatcaaggt tctttttcct tcattaactg ctgattcaaa
     3301 ccctaacatt tgcgcgcacg tacttttacc tacgccacct ttaaaattac cgattacata
     3361 tttcttcgct accacgttcc tacctctttt cttttagcaa tattcatttg atactattat
     3421 aacatattta tttttagtta ctaccaaaaa aaagatatat atgtactacc ttataattaa
     3481 taagtaaaag gtagtacata aataaggaaa agctgaaata tattgattta tagctttcct
     3541 caaagtaatg acgttattta atttatcgag aagcagagaa tactcgcgac tttagtcatg
     3601 tagacgaatc agattttcga taaaaaagaa gccgtccttc ttaagaacga caacaaatca
     3661 ctagattaat tttttcaact gttcataaaa gttttcatgc gttccacata acaatactgg
     3721 aataaccttg ttttctataa tctcgtaagc tactctatat gttgtgcccg catatttgaa
     3781 cccactagac caaatacctg ataaatcccc agttttttgt tcacccttat atggatcaat
     3841 cgcaatttca tcataaatta aattcaaaaa tttttctttc aatggcttct cttttagctt
     3901 ttttaaatac ttaatgacat gttttgaagg aacgatttca taagccaatt tctgttgcca
     3961 actcctcttt agtcattact ttagaattag taagtgtatc ttcagcaata tctctaaagg
     4021 aagaatgcat tttgtttttt ctattcttaa attctacaag aatctcatct ttatcgtagc
     4081 cttctgcaat tatatcagac aaaatatctt cgctaaaatc ataaaattct ttttgtggtt
     4141 caacaaattc ataaagaata ccattctcta ccaactttgc ttcaacttcg actccattag
     4201 gaacattaaa atctttaggt actgtcaaca tgattgaatt gccttgtttt cttgtttttg
     4261 taatcattta aatccctcat ttctaacagt atgtaattac ttagtaaata cattatacca
     4321 cgagataatg tatttacaag taattactgt gactcgtaat gcgtccttgc tgaataaatt
     4381 tcaaccacgt gttcatcttc atctacctta taaacaaccc ggtgttgtct attcaatctt
     4441 ctaaaatacc taccttcatg tgtaggtttt aacttctcaa atgattgggt tctgttgcaa
     4501 agttttaaat aaagaataaa atcccttacg gtatctatga tttaagct
//
LOCUS       DAYYHX010000190         4522 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000190 DAYYHX010000000
VERSION     DAYYHX010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.191"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..1164)
                     /gene="xerS"
                     /locus_tag="ACHA6Y_002436"
     CDS             complement(79..1164)
                     /gene="xerS"
                     /locus_tag="ACHA6Y_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM8988501.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1513..1708
                     /locus_tag="ACHA6Y_002437"
                     /pseudo
     CDS             1513..1708
                     /locus_tag="ACHA6Y_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(3240..3758)
                     /locus_tag="ACHA6Y_002438"
     CDS             complement(3240..3758)
                     /locus_tag="ACHA6Y_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM8988502.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4125..4373
                     /locus_tag="ACHA6Y_002439"
     CDS             4125..4373
                     /locus_tag="ACHA6Y_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988503.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1489 a          744 c          683 g         1606 t
ORIGIN      
        1 cagcttaaat catagatacc gtaagggatt ttattcttta tttaaaactt tgcaacagaa
       61 ccctattttt tactacattt acaaagcgtt caaagcatca attgtagctg cattgtctat
      121 atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg cagttaatac
      181 caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt tatgtggact
      241 gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg caataccgga
      301 tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt tttctgttac
      361 tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg caaatgctgc
      421 tatatttact gcgtctctct tatttccttt tctagttact accacattat tcttttctaa
      481 agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag atcctaaaat
      541 caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca aagctcgttt
      601 gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg tatcttcgtc
      661 tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt tatctgtgat
      721 agttgctact tgatccatag gattattttc taagtatggt tttcctaaag gattattgga
      781 acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat tgatagtaga
      841 agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct ctttttttac
      901 attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat aggcagttaa
      961 ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa gccattcata
     1021 aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat ctttatcctc
     1081 acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg tatctatttt
     1141 ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg gagaggagta
     1201 tctctataat tggactatac caattataga gtgttttgtc aattggtata ctctgaaatt
     1261 tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt aaaataaatg
     1321 gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa attgtctaaa
     1381 ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac ggttgatttg
     1441 atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag aagttaaaga
     1501 aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt tcttaaagaa
     1561 aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca agcaacaatt
     1621 caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa caaagtacga
     1681 tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa aagaaaaatt
     1741 tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg tattttttag
     1801 gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa aaccactagc
     1861 tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc ttactgtctt
     1921 ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat acttctgcta
     1981 agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc cttgccataa
     2041 taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc attttagtac
     2101 atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag aaaaaaactt
     2161 attaaaccag atataacttg taaacatcca actatataga aaacgtaaat tttctatata
     2221 gttggattaa gataacgact atttatttta tcatattttt actataccct atacttttac
     2281 ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt aagttagatt
     2341 aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt ataaattgat
     2401 ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt gttgacgatt
     2461 gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact tatgaaatga
     2521 aagacctttt acatcttgca gaaatcgtta ctgggattaa actaagcaat aaaaactaat
     2581 gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa atgacgattt
     2641 caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca gtatcagtaa
     2701 taggataggc gataggccac ataccctttg atggttagaa taaactgctc aaaatctgta
     2761 tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac cataagcgtt
     2821 gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat aattaccgga
     2881 cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat tgtcggtact
     2941 atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta gtctcctaag
     3001 tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc atggtttatc
     3061 tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat tctaggggag
     3121 tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt agtcgtatgt
     3181 tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta aacgggaatt
     3241 tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa tggagcaact
     3301 gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc ctcgtctgcc
     3361 cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat catcatgtta
     3421 atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag atgtgcatag
     3481 gtatttgtaa accacttata taactctcct aactgattcc atttaaattg atcagaaggc
     3541 gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa attcgtccaa
     3601 ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac tcttttatcc
     3661 ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt cctatttatt
     3721 tcttttttta attcttcttt atttttatat gttctcatta tattacctcc ataaattcct
     3781 aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg ttctacaatg
     3841 catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa atatcccaac
     3901 gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa tatatttctt
     3961 ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa aatcatgtta
     4021 actactatca attactaata aaaactacca ctttaagcgt taagaactgt taagtttagt
     4081 taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta aaactcaaaa
     4141 tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt aaaaaatatt
     4201 atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa cagctacaaa
     4261 aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca tattcacgcc
     4321 tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt taggaaattg
     4381 aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca ccagatattc
     4441 ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac cgcgaattta
     4501 ctaaaattat gaataattat gt
//
LOCUS       DAYYHX010000191         4516 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000191 DAYYHX010000000
VERSION     DAYYHX010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.192"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..420)
                     /locus_tag="ACHA6Y_002440"
     CDS             complement(<1..420)
                     /locus_tag="ACHA6Y_002440"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM8988504.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFA"
     gene            complement(579..1037)
                     /locus_tag="ACHA6Y_002441"
     CDS             complement(579..1037)
                     /locus_tag="ACHA6Y_002441"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM8988505.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1208..2353)
                     /locus_tag="ACHA6Y_002442"
     CDS             complement(1208..2353)
                     /locus_tag="ACHA6Y_002442"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM8988506.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2366..3283)
                     /locus_tag="ACHA6Y_002443"
     CDS             complement(2366..3283)
                     /locus_tag="ACHA6Y_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM8988507.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3711..4274
                     /gene="efp"
                     /locus_tag="ACHA6Y_002444"
     CDS             3711..4274
                     /gene="efp"
                     /locus_tag="ACHA6Y_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM8988508.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1410 a          834 c          853 g         1419 t
ORIGIN      
        1 tgcaaatcgt tcgattactt tgccatttct atcaatcaaa aatttagtaa aattccattt
       61 tattgcttta gtgacagtac caggagcttc atttttaaga taagtaaaaa gaggatctgt
      121 attttctcca tttacgtcaa tcaattgatg catagggaag gtaacgccat aagtggtttt
      181 acaagcttct gctgcttcag tcgctgaatc tagctcttgt ttaaattgat tggaaggaaa
      241 gcctaagata accagacctt gtgtctgata agcttggtaa agttcttcta attgtttaaa
      301 ttgaggtgct agaccgcatt ttgttgcagt gttaacaatg attatcggtt tgcctgcata
      361 ttttttcaat gaatagacag tgccgtcttc taaagtagtt tcaaaattat aaatagacat
      421 aaagttttct cctttctatt ttatataata aaaagagcgc taaactgaaa actagctatt
      481 tgttttcccg tttagaactc ttttcagaca ataaatattt aataaaatac gaagataaga
      541 tagctatatt gcttctcatc tttacgttct tttagaaact atgcttctac ttcagaccat
      601 tcatcgcgtt ttgctaaaaa ttcacgagcc aaagcttgtg caccttctag tgtatggctg
      661 gcagcccagc cacattgtgt ttcattgctt gcaggtactt cagtagcttc aagaacatct
      721 ttcattgttg cttcaagaac atctaaaatg tcttcgtagc tttcatgatt caatacagtc
      781 aagtaaaagc ctgtttggca tcccattgga gaccagtcta cgatgtaatc tgcgtgattg
      841 cggattagtt cggcagtcaa gtgctcaagt gaatgtagac ttggcatgtc catgtgttct
      901 ttatttggtt gtttaaagcg gacgtcgtat ttaatgatga catcgccatg cgcacccgtt
      961 tttcgatcag ctacacgtac ataaggtgct ttgacttttg tatggtcaag attaaaactt
     1021 tcgacattca ttctcatttg taagactcct atctgtttgt tcattcgttt tgatgctttt
     1081 cactaaagaa ttatttagtg aaatttacaa aaacgtcttt ctacttgatt ctaacactta
     1141 tcgtttccaa ggtgtagcaa tctgaaatat tttttagatt tttctgctaa gtcgtttatt
     1201 gggacttcta gttcttgatt ctgtcaaatg cctgagctaa atcgtttatg agatcctgac
     1261 catactcaat tcctacagaa agacggatca agccgtcctt tatgccagcc tcttctcgtt
     1321 tttcttttgg aatagacgca tgagtcatga cagaaggaat ttccactaaa ctttctacgc
     1381 cacctaaact ttctgctaaa gtaaatagtt ggagtgattc aacaaatgga atagcagatt
     1441 cttcattttt taaagtaaaa gaaatcattc cgctaaagcc gctcatctgc tgcttagcaa
     1501 ctccatggag ttcatgatcg ggtaatcctg gatagtagac cttttctact gctggatgag
     1561 aaaatagaaa atcagctacc acaaaagcat ttttttgatg ttcctccata cgaacactta
     1621 gtgtcttgat tccgcgctga agtaaccagc tatcttgggg accaagtacg gctccgatag
     1681 cattttggta aaatccgatc tgatcggcta gatctttatg gttagaagtc actaaaccag
     1741 ctactacatc gctgtgtccg cctagatatt tggtaccgct atggacaacg atatcagaac
     1801 cgagatctaa aggtctttga aaataaggag tagcaaatgt attgtcagca ataactatca
     1861 atccatgttg atgagccaga gtggcacttt tttctaaatc agtaatcttt agaagcggat
     1921 tgctaggtgt ttcaagataa agtgcttttg tattgggttt gatggattgt tctattttat
     1981 caagattact cgtatctatg atcgtgtact cgagaccatt tttagtaaga actttgtcaa
     2041 atagacggaa tgttcctcca tagacatcat ctcctaataa gatatgatcg ccagcttgaa
     2101 aaagagaaaa aacggcatga atcccagaaa gaccagaaga aaaagcgaat cctcgaacac
     2161 cgccttccaa atcagcaatc agttcttcca atgcaaaacg agtaggatta ccagatcttg
     2221 aatattcata ttgtttaggc tggccgacgc catcttgccg ataagtggat gtttgataaa
     2281 taggtacact gacagctcct gttgtgggat cttcactgat gcctccgtgg atcaattttg
     2341 tttgaatgtg catgtttctt tcctcctata agtaaatgtt ttttgataaa tagcggtctg
     2401 ctgcgtctgg aaaaatcgta acgacacgat gaccaggtgg taaacgacga atttctctta
     2461 atgctgctgc aaaagccgca ccactcgaac ttccaactaa tagtccttgt tctcgtgcta
     2521 actgcctagt atagttaaac ccttctacat cagagattgt ctcgatctga ttgattgaaa
     2581 ggggagaaag aaacggcgga atgaattcta caccgatacc ctcaatttcg tgaggggctg
     2641 gatcaccacc atttaacaca gagccctctg gttctacacc gattattcga atgtcgggat
     2701 atttttcttt caaaaaggta gaagttccag caaaggttcc accactccca attcctgcta
     2761 caaaactatg gatattttct ttgatttcat gaaagatttc tggccctaac gtatgatagt
     2821 aagcagcagg attatcttta ttttcaaatt gaagaggcag atagctgttt gatatctcct
     2881 ctgcaagttt tttacttttg ctgatggcac cgatgattcc ttgctcacta ggagaatgga
     2941 tgatctttgc tcccaatgct ttcatcaatt gttgtttttc taagctaaac ttctctggaa
     3001 caacaaaaac cgttcttaat ttgtatgtga gtgcggccag agcgatccca attcctgtat
     3061 ttccggcagt tggttcgatg atcgtcgttt tttcattgat ttttccttgg gcaaatcctt
     3121 ctttgatcaa ataactgccc aatctatctt tgatacttcc tccaggattt aaatattcta
     3181 gttttgcata aatagcagaa cctttaggca tagaatagtg ctgattgtca aagcgataaa
     3241 gcggtgtgga accaatagct tctgtaattg tcgagataat catgtgggcc tcctaaaaaa
     3301 agataataaa aaaaggagct gactattaaa tcagctcctt gaaagacata gagaaaaatt
     3361 gagtagataa aaagataagt tacctggtac tcattctatg aagggcaatt aatagacatg
     3421 acaaatagta tgctgctttt atgcaccata caacagcaac gattcaattg tttttggttg
     3481 ctgactgtca tgtgtatccc tccaagtaaa atttttatta acgatagcat gaaaaaaaat
     3541 aaaagtcaaa ggcttgaaag aaaaaaatat ttcgatagca tcgtttaccc gtgtttattt
     3601 ttctgacaaa taggcatgag accttaaaag atagagaata ctctagtttt ctggccggtt
     3661 gtctgataca ataaagttga ttatgataaa gaaaagaagg agcaaaaata atgatctcag
     3721 caagtgattt aagagccggt atgactttcg tacaagacgg caaactgatt aaagttctgg
     3781 atgcaagcca ccataaacct ggtaaaggaa atacagttat gcgtatgaaa ctgaaagatg
     3841 tccgttcagg tgcaacatac gacacaacgt tcagaccaga agaaaaattt gaaaaagcac
     3901 atatcgacac aaaacctgta caatatttgt acacgatgga tgatacagct ttcttcatgg
     3961 atttggaaac atatgagcaa tatgaaattc ctgtagaaac agtagaagaa gaaatgaaat
     4021 atatcctaga aaacatggaa gtaaaaatcc aatttttcgg tagcgaagtc atcggcgttc
     4081 aattaccaac aactgtcgta ttacgtgttg tggaaacaca accttccatc aaaggtgcaa
     4141 cagtcactgg ttcaggtaag ccagcgacaa tggaaactgg cttagtagtc aatgttcctg
     4201 acttcatcga aacagatgaa ttgttggaaa tcaatacagc agaaggttct tatgtaaaac
     4261 gtgcgtcaaa ataattcgct aacggattga taagaatttg aaaacagagc ttgacctctt
     4321 gaaaggcaga tcatcggatc tgcctttcaa gaggtttttt gtggaatcag caaattgaaa
     4381 aataaaagag tatttgattt tatttatatt ttgaaagatg aatggcaaaa gctagagaaa
     4441 gaaaaaactg cacttattta tgaagaaatt gaaatgattc ataaaattga aaatatccga
     4501 actattccga actata
//
LOCUS       DAYYHX010000192         4472 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000192 DAYYHX010000000
VERSION     DAYYHX010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.193"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            180..956
                     /locus_tag="ACHA6Y_002445"
     CDS             180..956
                     /locus_tag="ACHA6Y_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM8988509.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            971..1174
                     /locus_tag="ACHA6Y_002446"
     CDS             971..1174
                     /locus_tag="ACHA6Y_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8988510.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1190..1528
                     /locus_tag="ACHA6Y_002447"
     CDS             1190..1528
                     /locus_tag="ACHA6Y_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFM8988511.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            1515..1694
                     /locus_tag="ACHA6Y_002448"
     CDS             1515..1694
                     /locus_tag="ACHA6Y_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988512.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(1737..2207)
                     /locus_tag="ACHA6Y_002449"
     CDS             complement(1737..2207)
                     /locus_tag="ACHA6Y_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988513.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            2294..2992
                     /locus_tag="ACHA6Y_002450"
     CDS             2294..2992
                     /locus_tag="ACHA6Y_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM8988514.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            3170..3511
                     /locus_tag="ACHA6Y_002451"
     CDS             3170..3511
                     /locus_tag="ACHA6Y_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988515.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            3504..4175
                     /locus_tag="ACHA6Y_002452"
     CDS             3504..4175
                     /locus_tag="ACHA6Y_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8988516.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            4181..>4472
                     /locus_tag="ACHA6Y_002453"
     CDS             4181..>4472
                     /locus_tag="ACHA6Y_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988517.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGR"
BASE COUNT         1660 a          668 c          892 g         1252 t
ORIGIN      
        1 agacaacaaa aaacactatt gatatgtaaa tgcagaccgc caagaaagct ttaaaatcaa
       61 tgtttttatg tcttatttaa ttatgctctt agtatagagt attactcaga tactgtcaat
      121 tgaatttaga aaaaagttgt taaatttgtt tacgaatata aaagaaagga atgagaaata
      181 tgaacacacc gcaaattttt aatttcgaac aaaacgaagt tcggacaatt ttagtaaatg
      241 atgaaccata ttttgtaggc aaagacgttg cgagtgtttt gggttattca aacactaaag
      301 atgctttgtc gcggcatgta gatttggaag ataagatggg gtcgcgaatc acgacctcag
      361 gtcaatcaag agagatgaca atcatcaacg aatctggttt gtacagttta atcttaaaat
      421 caaaacttcc ctctgccaaa aaatttaaac gttgggtaac aagtgaagtg ttgccagcaa
      481 ttagaaaaca tggaggttat ctaactccag aaaaagtaga agaagctttg cttaatccag
      541 atacaatcat tcaattagca actcaactaa aagaagaaag aactggaaga ttaatcgcag
      601 aacaaaagat tgcagagtac gaacctaaaa tctcctattt agatagcata ttatcttcta
      661 cagattcagt aacaattagt cagattgcag cagattatgg gatgtctcca caacagatga
      721 ataaattact tcataaacta ggtattcaga aaaaagtcgg taaccaatgg ttattgtgca
      781 aaaaacacat gaaccaagga tacacaaaat ctcatacaac tgagatcccg aaagccgatg
      841 gtggcactaa aattgtaatg aataccaaat ggacacagaa agggcgtcta tttatctacg
      901 aattactaaa aaaagaagga tattaccctc aaatggattt agaggaaatt ggttagaaag
      961 gagttttagt atgactgaca ttgcagaaat cactcaacga gatagagaaa aaatcaaaga
     1021 atatgtcgaa agttcaaagt tcttaactta caccatgctt gctgaaagat ttggaattag
     1081 caaaagctac ttatctttaa ttttaaacgg taaaaagact tctgcagaag caaacagaat
     1141 tatagattcg attatcacta tgtacgaatt gtaaagggag gaacagctaa tgcaatatct
     1201 agaagcaaaa atcccaattc cagaaggcta tgtaattatc tcccaagtgg attatgagga
     1261 gttaaaaaaa gctgatgata ctggtagatg gatgacgttg ccagaagtgc tagaacggat
     1321 taacagaaaa tatgattggt ttacttctag agttttaaag aacccaagat atagaaatat
     1381 tatcgatatc gagaaaaaca aaaatggatt tgtctattat ccagttgaag gaagagacac
     1441 atatctattt ttaagaagta aaacacttga atttttagaa acaaattttt cggaaatctt
     1501 aaggaggcaa gcggatggga aaatttaata gagcgctagt gttcagcgca ccgctaatca
     1561 tctacgcttt aggactttgg ggaagcaggc aagcgttgat aggaacgatt gtttacatgg
     1621 tttggatttt tatggggctt gatgaagctg aagctgagta cagagcgaaa aagccaaccg
     1681 agggggctga ctaaaaatga atagaaaaga gaaactagaa tgtatattac tattactcag
     1741 tttaattctt tcactaattt ctctattggg aagtttgtat ttttgatatc aacaattttg
     1801 tggatttggg ttctatttag agtaaatatc atttttgttt ggtgattaag gagtttatta
     1861 aaattaattt tccctacttc aaatgcaagg taacaagata tagcactttt aggaggaata
     1921 tttagcggta cagcttcaga gtatattcta ttatcatcga ctgatcctcc tttagcaatt
     1981 aatttcttaa atggagtagc tgaccatctg cggtttaagt tagcggatga aagttctaaa
     2041 ttaactaaca tagctggttc tgttgaatag ttagaaatga ttacttttgt ataaatcata
     2101 tctgcagcga agtaagaagc attaagttca acttctagtt gagggcgcat tccttttatt
     2161 tttatccctg ttaaaaacgt actcaatata aaacttatta ttgacatcca ttgaaaaatt
     2221 gttaagtgaa aaaaattcaa aataaacacc accagttttt aactaaatta taccaaaaag
     2281 gagagaagaa ataatgcaag aattagtaat tttgaaaaat aaagaagctg tgactacgag
     2341 cttacaagtg gcagaaagct tcgagaaaaa acatcaacat gttttaagag atattgatgc
     2401 actaaaaaaa gatgtgtcca attttggaca gatgtttgta gaaggtaatg aaccagattc
     2461 atatggcaga aatcgacgag ttttcttcat tagtagagat ggatttttct tgctggctat
     2521 ggggtttaca ggaaagaaag ctatctactt caaacaaaaa tacattgaag cattcaacga
     2581 aatggaagat gttattcgca agaatactgt tcctcaaaca attgaagata tgatgatcta
     2641 tcaattagaa gaaatgaaag atgttaaaaa agatgtttcc atgcttaaag atactatgcg
     2701 aattagcgga caacaagagt ttgaaattaa gcaaaaagga aatatgaaag ttatgggagt
     2761 tctaggggga aaagaaagcc gagcttatga agaaatcagc aaaaaagtat tctcaaaatt
     2821 ttggtctgaa tttaaacgta ccttttcaat cccaagatat ggcgagttac ctcgtaagag
     2881 attcgatgat gctgtttcat ttattgaaat gtggttgcca gaaactgcga tccgtatgga
     2941 aattgatcaa ctgaacagac aacagagact ttttggtgat gacaatgaat agagctgaag
     3001 cgctaagaat agggacggca attgctaatt gctggtggaa atactataaa ccaatcatcc
     3061 taagccaaca acatattgac aagcaaaaag catggcaaca aataaaaaag tgactccgcc
     3121 ggcaagcaaa gagtcacaaa gaaaacacat cataaggaga ttttagcata tggaaaaaga
     3181 actttccact ctagatcaat atttgattga tcctgattgg ggcaagccga aaattgagga
     3241 aacaagtggt cgaaaaatca gacgaaatct tttgacgaat gaagaactag cttgggatca
     3301 agatgattta ggcaaccatg taactatttg ggatcatgtt tatcttatcc atctatcgaa
     3361 gcattcgaat aaacctgaat atatttacgt catcgaagat ggcttgattg atgcgctaga
     3421 agagtacgac agagataact tgattgatat ctcttattac ggaccaggta agaaatacat
     3481 tgctgaaatg gaggcagaat ttgatgagtg aaatcaaagg gacaacgaac tttgaaaaac
     3541 tttttagtcg taagttaaat aaaattctca agaaaaaagg aaattttgat tatttatctt
     3601 gggctcacgc gtgggagatt atgaaaaaga atgatccaca ggcaacggta actattaatg
     3661 agtacaaaca ctacagggtt gtttctggaa ctcatcaaga ctttcttgtt gaggaatata
     3721 aaccttttct tatggatgaa actgggactt atgtatctgt ctcagtaacg gttaaaggac
     3781 acacggaaac cgaattattt cctgttttag attatcgaaa ccaaccagtt gttaaaccaa
     3841 atgcaatgca aatcaataac tcattgaagc gatgctttgt gaaagcattg gctctacacg
     3901 gactgggatt atatgtattt caaggggaag atattccaac accacctaga atcgatacaa
     3961 agaaattaaa catgctagag acgattctag aagctttcaa tgagcagatg ggtaaagata
     4021 tgaccaaaac cttaatcgaa tatgttaatg agcagacaga taaattaggg ctcttagctg
     4081 ataacgttga aactattgaa cagttaagct atgagcaatg tgccttgatg gagcgagcaa
     4141 tagcagctaa gagaaaagaa ttagataaga agtgatatga gtgtttaaac cattaatcga
     4201 ttcatattca gcggttctga aaaagttcaa aggaaaagac ataagcgcaa ccatcaatga
     4261 ggaagtgaac attgatcgac taaagacgat gtatgacggc tacgatggtg atcgagtcat
     4321 tgaaattcgt tttattgatc ctagacgttt caccgtacag caacgaaact tcatctatgc
     4381 gctgataggc gatattttta tcgatacagg catgccaacg gacttctgga aggaattctt
     4441 ctactttcgt tttgaaggtg tcacagggcg ca
//
LOCUS       DAYYHX010000193         4446 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000193 DAYYHX010000000
VERSION     DAYYHX010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.194"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..2711
                     /locus_tag="ACHA6Y_002454"
     CDS             42..2711
                     /locus_tag="ACHA6Y_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM8988518.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2802..3416)
                     /locus_tag="ACHA6Y_002455"
     CDS             complement(2802..3416)
                     /locus_tag="ACHA6Y_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8988519.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3536..4075
                     /locus_tag="ACHA6Y_002456"
     CDS             3536..4075
                     /locus_tag="ACHA6Y_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988520.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4072..4410
                     /locus_tag="ACHA6Y_002457"
     CDS             4072..4410
                     /locus_tag="ACHA6Y_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFM8988521.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1377 a          776 c          838 g         1455 t
ORIGIN      
        1 aaactgaata caacttcata gaaatttggt ggaaaataaa tatgaaaaaa aatttaaagt
       61 cattctttag agaaaacgga cgctacgcag cagctagttt cctgattccg tttttgattt
      121 tagcacttgt ttatctaagt atcgggatct atcctggaag cagcagaagt attttggcaa
      181 gtgatgcctt ttcacagttt tctaattttc atgccagctt ccgaaatgtt cttcttggaa
      241 agcaaagtat tttttataca tggaatgctt ctctaggatt gaattattta tctttaattt
      301 cttactatct aggcgggatc ttcacgccac tggtgttctt ttttccaaac cagatgatgc
      361 cagatgcact gtatgttctg actttgctta aggtcggctg tgccggattg gctttttggt
      421 ttcttgcacg tacgttcaag attcctcgct gggggcatgt tgcacttagc gtcagttacg
      481 cattgatctc atttatcact gcacattcgg agatcatcat gtggctggat gcatttgttt
      541 atcttccatt agtgattttg gggattcatc gtgttatgga tcttaaacgg ccgaaacttt
      601 tatttatcag ttatttactc ttgtttttat ccagttttta tatgggtttt atgattggcg
      661 tattttcttt cttatatttt atcgcccgtc tattgacaaa ctggtcagaa tataaaaaaa
      721 cgattgttcc ttatggtatc acttctttac tggcaggcgg cgcttcaatg atcattgtat
      781 tgccagcagt gctggatctt cggtcaaatg gcgaggcact gagtcagatc actacgttga
      841 aaacagaagc gacttcttat ctggatctaa tcatgaaaaa tatgattggt gtttatgaca
      901 cgacaaaata cggatcgatt ccgttcgttt acgcaggtct gctaccatta gtcctctgtc
      961 tgttttattt tgccagtcgg aaagtaccgt tgaaaaataa atttttgtac ggcagtcttt
     1021 tcgccatact tatcgcaagt ttctatcttg ttccattgaa tttgttctgg catggtatgc
     1081 atgcaccgaa tatgttttta ttccgttatg cctatctgtt ctctttctta gtgctcctgc
     1141 ttgcaggaaa aggatgggaa aagatagaac aagacgatcg tgggctgttg ttaggaatca
     1201 ttttggtcgt agctgctctc tttgcggtgg catggggcat caaacctgaa ggaagctaca
     1261 cctatgtgac gatgacttcc tttgtattga cagtggtttt cttagggatt tatgcttttg
     1321 ctgtgggatt ttatcagcat aaaaaacttt cattcaatca tctgagtata ttgttattat
     1381 tgatcatgtc tgctgaagca ctggtcaata cgaacagtat gatccatggc attcttgatg
     1441 attggaatta tgcttcccgc agtttatata ctgatcctta tcctgacttg aaagctcttg
     1501 taaatgaaac aaaaaaagat tccgactcgt tttaccgctt ggaaaatctg gatccagtat
     1561 ctgcaaacga ttcgatcaac tatggttaca gtggtatcag tttgttttct tctataagaa
     1621 atcgtaattc ttcctcttac ctggataaat taggtttccg ttcaagaggg acaaatttga
     1681 atatccgtta taacaataat actttgctaa tggatgggtt tacaggaatc aaatataaca
     1741 tttctaaaaa cgaaagcacg ttcagtaaat atggcttccg ccaagctggt caaagtggcg
     1801 actatacact gtatcagaat gtcaatgcat tgccactagc atttactgca ccgttgtccg
     1861 ttaatgatgt tgagcagcca gtgacagata atttgaccag tcagacaaac ttgatcaata
     1921 cattgtctgg tttaaatgaa cagtatttta cattctatac acctactttg aagaatcagt
     1981 tgaatacgac gatcacaaag acaacgactg gcgtcactta ctcagaaaca gcagataacc
     2041 agccaaaaga gctcacttgg gaagtccatg tgcctgcaaa tacacaagct tacttaagtt
     2101 tgttcccaac caattttgca caattagaaa gttctagtgc gacgatcact gtcaatggta
     2161 catcgcgaaa aacgcaaatc aacatttctg gtcaatacta tgatcttggc tattatcctc
     2221 aagagacaac agtgacattt actgcaagtt tttatggaac gaaagaagta agtttcatgg
     2281 agcctaaagt cgttggtcta gatgttgtag cctatcaagc cgcaatgaat cagatcaaag
     2341 aaaaaggtgt cgaaatggaa gccactggca gaaaagcttc tgggactgta aataccgctg
     2401 aagattccat gttggtgaca actattcctt atgacaaagg atggaaagct aaaattgatg
     2461 ggaaaccagt caaggtcagc aatttcaaag acgcattctt gatgataaaa gttccagcag
     2521 gtaaacatac tgtcactttc agttacttgc ctgaaggatt tacgataggt gcaatccttt
     2581 ttgtcctatg tattgcattg tttattcttt atgtctggta ccttgaccgc aatcgcccct
     2641 tttatggcac gattgatcca gcaatagaag aaaaaccgcg aaatacacac agaagaagac
     2701 gaagaaaata aagaaacgat ggaatctgtg tcactcttct atttaaatgg caaaaatttc
     2761 aatctttaaa tgacaaaaac cacctgattt ttggtggttt tttatatacc tatgactaaa
     2821 ctatctcttg ctgcttcgac aacttctaca gtgatcttat ccatttgatt gattcttaaa
     2881 atccgatcaa tctgcgcaaa taaacgatga ataagcctaa aatttccttt tgtgatctta
     2941 atgatagttg tgatagcctc ataatcagaa aaatcttcta gcttgatatc aattcctaaa
     3001 tctgcccatt tgtattccaa aatatgatgt gtttcgtcct tactaaggtt atcaaattca
     3061 tgtgcaaatc ctatgcgaga gtaaagctgc ggataacgag acaatctttt ttcaatacca
     3121 ggcataccga taagaaccat tgccagatca tttctatcgt aaatatctct tagctgttct
     3181 aaatgctgaa gctttaaacg atcaatttca tcgacaataa ttaagtcaat tgcttcatag
     3241 gttttaggca caggaggatc ttcttgattg tattttagta ccatatgcat tgctttagtc
     3301 agacctattc tactagttat tgtgttaata tccgaactga cacgagacat cttttcagct
     3361 ggtacagtat aaaaaacttt atttacactt aatatttcat ctgttgcatt tatcaagaac
     3421 aagaaaaagt ctgaatattc aaaaaaaata acggtgtaca tttttaaaag gagatgtata
     3481 atcgagattt agaaagcgtt ttcctcaaaa tttaatgaat taggaagtga ttactatgaa
     3541 aaaattattt agcgcacttt ttattctgtt atttggcttt attggtattg ggacttctgg
     3601 aaatatagtt aaagctagca cgatggttga acaaacaagt gaaatggcaa aaacttttag
     3661 tcaattaaca ccagaagaac aaagttactt caaatccaaa ggatttagtg ataaagatac
     3721 tttttacact caaaagatag agcctcaaga tccgaaaaca cgtttttctg caaatgtatt
     3781 gcggttaacc gcatctacta aaaaaattac taatacgcag gggcggacag aatatattat
     3841 aacaagtccc aattcggcga tgtatcgtat ttctacacgc ttgagtttag gtagtgcaaa
     3901 atcatatact caaacagtaa atctagctaa ttcttatggt tatggtggag gaatgtattt
     3961 tacatttact gggaaaagac aatattacag tgtaaaatta gctatgcaag tttataatac
     4021 atttggtgta gggtcagtct cttgcactgc gggaggattg acaataggaa aatgataatt
     4081 tcttactatg tcttaaatag aagtgatgat gaaggactct tcgtttttca atcattacat
     4141 gaggatacga tgctaaaaga gttttataat cggattaaga ataaacagat agaaacaaat
     4201 ttatattttt taagtgactc tatcttgcct tttattagca accctaaaat tcttaacttg
     4261 ttgcagacaa aagatttgaa aagtttaccg attattgtca ttgatgagga aattgtaaaa
     4321 tatggcgctt atctaagcgt agaagaatta gaaaaattaa ctaacattgg aatgagtatt
     4381 caggaggata atggggtaca tggaacatag aatgaatatg cacagttgag aacggaattt
     4441 gacatt
//
LOCUS       DAYYHX010000194         4390 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000194 DAYYHX010000000
VERSION     DAYYHX010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.195"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..338)
                     /locus_tag="ACHA6Y_002458"
     CDS             complement(<1..338)
                     /locus_tag="ACHA6Y_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988522.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEF"
     gene            complement(546..1472)
                     /gene="glsA"
                     /locus_tag="ACHA6Y_002459"
     CDS             complement(546..1472)
                     /gene="glsA"
                     /locus_tag="ACHA6Y_002459"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM8988523.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1495..2922)
                     /locus_tag="ACHA6Y_002460"
     CDS             complement(1495..2922)
                     /locus_tag="ACHA6Y_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8988524.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2953..4167)
                     /locus_tag="ACHA6Y_002461"
     CDS             complement(2953..4167)
                     /locus_tag="ACHA6Y_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM8988525.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1380 a          928 c          776 g         1306 t
ORIGIN      
        1 ataaattcaa gatcagccag ttctgtactc gtgaatatac cattaaaata ataacttaaa
       61 aactttaaaa aataagattg accgtacaga aactgtgtga gctcagaaag agcaatacca
      121 tgtttgaata cgagtttata cttatgcttc tctcttactt gttcttttat acaatattct
      181 agacactcgg ctagactttc tagatcgtga aggatcgttg gtgtagtcac ccttagagaa
      241 atagctgttt gttcgatgtc tatttcttgg aattcaaata attgttcaca cagatacact
      301 tttctaaata tatttcgctc gatgtaattt tccagcattc ctgatacacg cctccagttt
      361 ttcttatgat tttatttaat catatctgta aatcgtattt ttgtttcctt tttatttaga
      421 aaagcccttt caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac aagctcacaa
      481 tttcagcctc attgtaggta gctttgagcc gtttggactt ttaccccagc ctctttcaag
      541 aatggttatt caaagatgtc agcgtctagt tctttgacta catctttcaa caattggata
      601 ccagctgcgc tgttaccaaa tggatctagt gccgggctga agacacctat gccgtaacga
      661 tttggcacag ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac accaacatgg
      721 acggaaaact ctcctgattc atcatacaaa ccagcagttg tcataattga tttgacgatt
      781 gtagcacttt cttcagtaat gatttgtttg ccattccaag gagcaattcc tttatttgcc
      841 agcacagcag ctaattttgc tatgcctgta gcggtgacat tgagggagca ttgtctgaaa
      901 taagcatcga gtatctccgg tacgtctcct tcgatcattt gattaccttt catatagtaa
      961 gcaagagagc gattgatatc acctgttctt gattcagaat gatagatttc ttcattcaat
     1021 gtgatattag gatcatcaca gatctctttc ataaatgaca agattcgctc aaatttttca
     1081 tctgcatctt taccggcaat caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga
     1141 ttcattggat gtttgcgatg attgatttcc atattcatga tagaattgaa ggcaaaaccg
     1201 gttggttccg tgttgattgt ttgaaagact ttttctattc cgttgtcttt gattgctaat
     1261 aacaatacgg gaacttttga catgctttcg atggcaaagc gcacatcggc gtctcctccc
     1321 tcaatcagtt gttttgtatc caaatcataa atcgccatac ctaattgttt gggatccact
     1381 tcagctaatg ctggaatgta atccgccact tgtcctgttt gataatagtg gaaatttttt
     1441 ccaatcaatt gttctaattc ttttacttcc atttgaataa ccagctttca ttttttattt
     1501 ttctgcatgc tggaatttat gacggcatgc gtagatcgta aatggaataa ctaagacgat
     1561 aagataagaa acaatcagaa tcgtttcata aatcccatgc tgtccagctg cgatactagc
     1621 aggtggaaca aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac
     1681 gattactttt acaacttttc cgcctggaac ctgataggct ctttcaagac tgtcatcttt
     1741 aaaaaccagc ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt agattacaac
     1801 agtcaatgac atcgctgcaa gaaatgaaag attattcccc ccgccgctta atgtaaagac
     1861 ggctgcccag attgttgcaa cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg
     1921 tgtgttttct tttgcaaaat attcaggaag taatccttct tctgctgcta cgtgaagtgc
     1981 ttttgaagga gaggttaccc aagaagcaat ttctccgatg acacctaaac aaacaagaat
     2041 cgcaagaatt ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata ggaattctaa
     2101 cgattgaatc acccctgtat taagagacaa accatgttga ggtactgtag tagcgacggt
     2161 caatccgcca aaagtatcta aaatgattgc cagaataacc aatacaaaca ttgctaatgg
     2221 ataatccttt ttagggtttt ccatttcatt tgcatgactg gcagatgttt ctacgcccat
     2281 atatgctaaa ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat
     2341 caatgttttc atagacagta tcgtttgtag tggagcgcct gaagcgacat aatggatccc
     2401 taagccaaac aagactaatg ctggcaacaa gataccagct acaaaaccga tccgagcaat
     2461 caatgaagtg tatttggttc cttttaactg tgagagtaaa atagcccaga aaataatcaa
     2521 taggattgcc aatttcagat atttattgtt ttgtatagca ccaatacccg ttgcatagga
     2581 taatgcacca gttaagaaat acagcatggt caagtagcca actgttatct caaaccattg
     2641 gaaaaagatt gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc aagtatagac
     2701 accgcctttt tcccatcctt tcaccgttgc catctctgct gcgcacaatg ccacagggat
     2761 aaaccatagc aaaccgccta ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa
     2821 tgtgggatat tcatataatg aaacgaccat tgagatggtc atagccaaaa agccaaagaa
     2881 cgttaatttt tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc ctcttttcta
     2941 tttgaatttg tttcaattga gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg
     3001 ttcttctttt gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt
     3061 cagcacatag gcatacacgg cagtaatgat gacaccaatc acttgaatcc ctaagaagta
     3121 ccagctgctt ggattcgtta gtaaatggct gttagatgca aagatcccta tcagaatcgt
     3181 acctgtgaat ccgcccatgc catgaacacc ccacacatca agtgcatcat cccattgttt
     3241 ctttttacga aaatcaacag cagcatgaca gacgataccc gcaatgatcc caatcagcat
     3301 ggcacttttt gcttctacat aaccggcaca aggcgtaatg gctgccaatc cagcaacgga
     3361 tcctgttagt acgtcgacaa aatcaaagcc atttcctttt acttttgcaa tgatcagcca
     3421 agtaatcatc gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga ctgcttgttc
     3481 atctgctcga agtgcaccac cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt
     3541 accgatagca gctgccataa gactgctatg ctgacttctt tctcctaaag gaatctttct
     3601 ttgaccaagt gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc
     3661 tccaccagcg aaatctctaa atcccatctg atccaaaaat ccgccacccc atatccagtg
     3721 gcaaacaggg aaataaatca gcagattcca aaaaataaca agtataatat agccttttaa
     3781 attcaatctc cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga taaacatcag
     3841 ttggtaaaga aagaacatta aaaatggaat cgttgcacca tgaaacatgt tggggaagct
     3901 tgtaacatgt ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc cgatatcccg
     3961 tccaaaggcc aatccgaatc caccaaatac ccacataatc gttgttatcc caacggagag
     4021 aaaagattga gacatgatcg ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa
     4081 agctagacca ggagtcatca ggcaaaccat tgctgtacat aaaatcatga atgctgtatc
     4141 tcctgtattg ataggtaatg tattcatttt tttcgcctcc tttctacgat tacgagtata
     4201 aaagaaggtg tagttgtttt tttcttctgt ttttttgaat taatacttgc atttataaaa
     4261 gacagacaat taataaaagc ttgatgtgtt gtgctctttt tcgtttttca cacaaataaa
     4321 gacaaaaaat aaacggtctt ctttttatag aaaaccgttt attttcgtct ttttttattt
     4381 ttaaagatat
//
LOCUS       DAYYHX010000195         4349 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000195 DAYYHX010000000
VERSION     DAYYHX010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.196"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            79..891
                     /gene="recO"
                     /locus_tag="ACHA6Y_002462"
     CDS             79..891
                     /gene="recO"
                     /locus_tag="ACHA6Y_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM8988526.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            1275..2192
                     /gene="glyQ"
                     /locus_tag="ACHA6Y_002463"
     CDS             1275..2192
                     /gene="glyQ"
                     /locus_tag="ACHA6Y_002463"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM8988527.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            2194..4269
                     /gene="glyS"
                     /locus_tag="ACHA6Y_002464"
     CDS             2194..4269
                     /gene="glyS"
                     /locus_tag="ACHA6Y_002464"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM8988528.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         1471 a          798 c          936 g         1144 t
ORIGIN      
        1 aacggcggaa gatcgaaaca taaataatct ggatcggcag aatctgcttt tccagattta
       61 ttttttagga gggagaatat gagccagctt gcagaatcga aaggcttgat tttatctagc
      121 cgtaattaca aagaaaaaga taaattagtc aaaatcttta cggaatcttc tggcaaaatg
      181 atgttttata tcaaaggaat ccatcggcaa aatcagccat tagctccggc tttgctcccc
      241 tttaccgaag cagtctatat cggtaatttt cgaagtgaag gtttgtcgtt tttaagcagt
      301 gtcaaagacg tccatgcgtt tcggaacatc caagaagata tctttttgag cggttatggt
      361 acctatttgc taaatctagt agatgcagca atcgaagatc atcaatatga cccgcattta
      421 ttccaattta cgcaacaagc tctgcagcgg atggatcaag gagatgatgc agaaatcatc
      481 acgaatatct ttgaagttca aatactgcaa cgcttcggaa tcgcgccaat ttggacgcac
      541 tgtgtcgtat gcggggaaac aaaaggaaaa tttgactatt cttccaaatc tggcggtgtg
      601 atatgcgaga aacactggcc aatggacgaa caccgttatc atgcagatcc tcgcgctgtt
      661 tatttcgttc gaatgttttc ggctatttct tatgataaga tcagtggaat ccacttgaaa
      721 gaagaaacga agcaggcaat ccgtgacctc attgaccagt tgtatgatga atatgtcggt
      781 attcatctaa aaagcaaaaa gttcattgat caaatgaagt cttgggcaca cgtattaaag
      841 acgccagaga aaaatatgcc tgaaaaagag atacctgaaa aagaagaata aatgaaagat
      901 acaacaaaaa aatcaaaact atttgacaaa tacagggact tccgtctatt attaaccgta
      961 gattaaatag atcgttcact gagaggatct attatataaa caagaaaact gcaaaatgaa
     1021 tacgtacatt gaccaagatc agtaaaaaac acgtggttta gcgagttcgg gagagtggaa
     1081 gccggaccca aacgattttt gaagaggcac ttgcgagtac gataaatgaa gctgccgaat
     1141 ctttattcgg aagtagggtg gaaccgcgaa attattcgtc cctatgtctg taatcagaca
     1201 tagggacttt ttgtcgtttt ctttcctgta ttcttattga cagcaacgct taaaaattga
     1261 aaggggcaaa acagatgagt aaaaaattga ccgtccaaga tatgatttta acattacaaa
     1321 aattttggtc atcaaacggc tgcatgctga tgcaagctta tgacacagaa aaaggagcag
     1381 gtacgatgag cccatataca ttcttacggg caatcggacc ggaaccatgg aatgctgctt
     1441 atgtagagcc atcccgccga ccagctgacg ggcgttatgg agaaaaccca aaccgtttgt
     1501 atcagcatca tcaatttcaa gtcgtaatga aaccttcacc tgaaaatatc caagagcttt
     1561 atttagaaag cttgcgtctt ttaggaattg atccattaga acacgatatt cgttttgttg
     1621 aagacaactg ggagaaccca tcgatggggt gtgccggtgt gggttgggaa gtatggcttg
     1681 atgggatgga gatcacacaa ttcacgtact tccagcaagt cggaggacta ccttgtaaac
     1741 cagttacttc tgagatcact tatggactag aacgtcttgc ttcatatatt caagaagttg
     1801 aaagtgtcta tgatctggaa tgggctgatg gtgtgaaata tggcgagatc ttcaaacaac
     1861 cagaatatga acattccaaa tattcttttg aagtaagtga ccaagacttg ctactaagca
     1921 actttgaccg ttttgaaaaa gaagctaaac gctgtatcga tgaaaatctt gttcatccag
     1981 cgtatgatta tattttgaaa tgcagccata cattcaattt attagatgct cgcggtgcag
     2041 tatccgtaac agaacgagca ggttatcttt cccgtatccg caacatggct cgtgctgtag
     2101 ccaaaatctt tgtggcagaa cgcgaaaaat taggattccc tttattgaat aaaaacacag
     2161 caaatccaga aaacatgaag gaggctgaat aagatggcaa aagacttatt acttgaaatc
     2221 gggctggaag aaatgcctgc tcatgttgtc acaccaagca tgaaacaatt agaagaaaaa
     2281 acagcaaaat ttttagatga aaaccaattg acttatgata cgatcgaaac gttttctaca
     2341 ccgcgccgct tagcaatcaa agtaacaaac attccagaac gtcaagcaga cagtgaagaa
     2401 gaagtcaaag gaccagccaa aaaaattgct ttggacgcag atggaaactg gacgaaagca
     2461 gctgaaggat ttgtccgcgg acaaggtctg acgacagatg ctatcacttt tagagaatta
     2521 aaaggcgtgg aatatgtcta tgtaacgaaa tacactcacg gcaaagaagc gaaagaagta
     2581 ttgacaggtt tagcagatgt cattaaaagc ctcactttcc cagtaacgat gcattgggca
     2641 gaatacgatt ttgaatatat ccgccctatt cactggatcg tggcattgtt agatgacgaa
     2701 gtaatcccgt ttgagatctt agatgtcaaa acaggcagag cctctcgagg acatcgtttc
     2761 ttaggcgaag atgtgacatt tgcgcatgca aaagaatatg aagaaaaatt gaatgaacaa
     2821 ttcgttattg cttcaccgca aaaaagacaa gaaatgatcg ttacacaagc aaatgaatta
     2881 gccgaaaaaa atcactgggt tattgatcta gacgaagatt tactagaaga agtcaacaat
     2941 ttagtcgaat atccaactgt cttttctggc gacttcgaag aaaaatatct ttctgttcct
     3001 gaagaagtac tagtcacttc tatgaaagaa catcaacgtt atttcgaggt acggaacgat
     3061 caaggtatgc tgcttcctca ttttatttct gctcgtaatg gtgatcgggt aaaattggaa
     3121 aatgtggttc gaggcaatca aaaagtattg acggctcgtt tagaagatgc ggaattcttt
     3181 tataatgaag acaagaaagc ttcaatcgac caatacgttg aaaaactaaa aactgttact
     3241 ttccatgaaa aaatcggtag tatctatgag aaaatgcagc gggtacaagt cattgcccgt
     3301 atcattggcg aacaagtagg actatctgag gaagaattga tggatctgca acgagctgct
     3361 gaaatctata aatttgatct agtaaccaac atggtagggg aatttcctga actacaaggg
     3421 atcatgggcg aaaaatatgc tctgctgaaa ggcgagaaac ctgctgtagc aacagcgatt
     3481 cgtgagcatt acatgcctat atccagtgaa ggagagctac ctcagtctac agtaggttct
     3541 gttttagcga ttgcagataa attagatagc attttttctt tctttgcagt tgggatgatt
     3601 ccaagcgggt caaacgatcc ttatgcctta cgtcgccaag catacggtgt cgttcggatc
     3661 atcgaatcga aaaaatgggc ctttcctttg tctgttctgc aagaaacgat tagtgatgtt
     3721 atcagtaaag atacggatcg tttcggcatt ggtttatcag aagggcaaca acaggtgatc
     3781 gactttatca aaggccgtct gcgccaatta ttgacaacga agaatattcg tcatgatgtc
     3841 atcgaagctg ttttgaatgc agaacaaaaa gatctgacaa aagtatttgc agcagcacaa
     3901 ttgttcaagc agcatcttgc agatgaagac ttcaaaccat cgatggaagc tttgacacgt
     3961 gtcgtgaact tagcgaaaaa agcggagctt gaaaaacaat cagaagtaga ccctgaactg
     4021 ttcgaaaatg aagcggagaa agaactgcac aaagcagtag aggcaatgaa aaaaagcttt
     4081 gctgaccaaa cgatcaacga aaattatgat cagcttgtaa gccttcgccc actgatcgaa
     4141 aattattttg atcagacgat ggtcatggca gaagaaccag cagtacgtaa gaaccgatta
     4201 acgcaattaa gtcaaattgc tgccatggct ttgtcgctag caagtttaga tcaaatcatc
     4261 acaaaataat ttctaatatg taaataaacc agagaatagg cacaaaatag gctatttctc
     4321 tggtttattt gttataaata taaataact
//
LOCUS       DAYYHX010000196         4324 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000196 DAYYHX010000000
VERSION     DAYYHX010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.198"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            445..591
                     /locus_tag="ACHA6Y_002465"
                     /pseudo
     CDS             445..591
                     /locus_tag="ACHA6Y_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(679..972)
                     /locus_tag="ACHA6Y_002466"
     CDS             complement(679..972)
                     /locus_tag="ACHA6Y_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM8988529.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            1009..1194
                     /locus_tag="ACHA6Y_002467"
     CDS             1009..1194
                     /locus_tag="ACHA6Y_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988530.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(1375..2553)
                     /locus_tag="ACHA6Y_002468"
     CDS             complement(1375..2553)
                     /locus_tag="ACHA6Y_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8988531.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2657..2971)
                     /locus_tag="ACHA6Y_002469"
     CDS             complement(2657..2971)
                     /locus_tag="ACHA6Y_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8988532.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3078..3293
                     /locus_tag="ACHA6Y_002470"
     CDS             3078..3293
                     /locus_tag="ACHA6Y_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988533.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3593..3835)
                     /locus_tag="ACHA6Y_002471"
     CDS             complement(3593..3835)
                     /locus_tag="ACHA6Y_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8988534.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3822..4250)
                     /locus_tag="ACHA6Y_002472"
     CDS             complement(3822..4250)
                     /locus_tag="ACHA6Y_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8988535.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1369 a          826 c          649 g         1480 t
ORIGIN      
        1 aaatactctt tttttacgat tattgaaaaa aaaagataaa acttgattaa ttgaagtatt
       61 gcttaaattt ggaagctatg agggaagaat aaaagagtta ggacgggatt atctactatt
      121 tttctttgtt ttaaatattt gttatctttg aacataaaag agaaatgtgt cgactgcagt
      181 gttgtgtccc tcatcacgaa taaacggtgt tcaattagct gatcttgtat tcacttaaaa
      241 aatggtaaag tacgagttgc tattccccat tttcaatttt ctaacgctca ggacaaaata
      301 ctgttttcac aacctcattg gaataaaagc atatatattg tttcaagaac gaaatcttgt
      361 tatcttttta aagggcatct gtccaaaatc taattttagg aggtacgttt tggtatttat
      421 tcgtacaaat aaaagagaat tatctttatt catgtaacgc atccaattga ttttttcatg
      481 tacgctcctg gaacgatttt actgacgatt gcttatttaa ctgcgaatcg ctcattagct
      541 tttatcatag ctatccatat tttgaataac gttcttggct ttgtattata gatacagatg
      601 acaaaaaaag ttcaagtatg cggtgtagaa ccaatatcgc atacttgaac ttttttcaat
      661 gagtataaat taaaacttct aattttcaat tcccatcatc tgatatgccc gaccgtctga
      721 acttcttttg aaaaagccaa atgtgattag ataacgtctt tgttcgacat aatcgccact
      781 agaaaatacg ttcagcagta tcgaattaac ctctgtttct gtataagtca ctccatggtc
      841 aaagcgtttc aagacttctt tcaataggat gtatttcttt tttgttgaac ttggtacttg
      901 ttttactttt ccatcattca tgaatgtgtc taaaactttc atcttttctt gctccgtata
      961 agtataaacc atcttctttc tccttttgat tctaagaata ctccatttat gttagaatat
     1021 tctggatttt ttattaaagc ggttactctt aagaaagata ttatcatagt gctctttttt
     1081 ttactactac ttataggtag atttattcta atccattatt ctcccctacc aataattaaa
     1141 gaaaaaactg gatcagggtg ttcaaattac atgctaagaa aaaagaaccc ttgaatacca
     1201 agggttcttt ctcagtctga taacaatgat taacgacgga tttctttgat acttgtgaaa
     1261 agtcgttaaa gtattgatat aaaaggattt ttattacctc tgactacata ttgactaatt
     1321 tgttaaaaaa tcgtttttca agaatcctgt aataccagtg ctttcaccgt ttttttaact
     1381 ctctaaagaa ctaaaagcaa tcagaggttt taccttttca tttgacgtta agtggcgata
     1441 aattttcgaa gttgtttggt cagtatggcc taagcgatct ttcaagactt cccagctaca
     1501 gtgttccgct actaagcttg ccattgtatg acgagctaaa tgagtatgaa atggcttatt
     1561 aattcctgag cgattcacgc accgtttgac gtgatcattt acatactctc gacgataagg
     1621 aacaccacgt ttactagaat caagatacac aaacaaatat gtttcttcta taataaacct
     1681 gtaatgttgc ctcatctcac ccaaagcctt aatcaactgt tgtagctttt ccataactag
     1741 aggagttact agaactcgtc tagcagaact aactgtctta gtagtttcaa ttcgaaaatc
     1801 cttactctta cctttaactg ccgtttgctt atagaacaga atttcatttc ttttgaaatc
     1861 tatatctcca ggctgtagag caagcgcctc gctgactcgg cagccagtgt aaaatagaac
     1921 atggaaaagt acagacatcg gtaattcctc atgaacaatg ccataattca aaaaagttct
     1981 caattcatcg acagtaagaa acttgtcctc aatattattt atggcttctt ttaaatcagt
     2041 taccgttaac tgatattttg gcattctcac agacttcatt ggattttcac ttatcaaatt
     2101 ctttctttga caatatttaa aaatcatatt tagaacttga ataagtgatt gtaaactatt
     2161 tttggcatag ccaacatttg ttccattaac gccacattcc tttaaggtaa aaagcaattc
     2221 ttgaaggcac tctggtgtca aatcagacaa ctttttatca ccaatccatc gaacaataat
     2281 ttttaattgt tcctctcgct tatcataggt tatttccttt tgttcattta acgaacggta
     2341 atattggaac cattgctcac ctacaacact aaacttgtca ttgcttgcaa caattttttt
     2401 cttaagcctt tgttgtttct ccatttccag ataagcgagt tgcgcctcac gttttgaatt
     2461 aaagccacgt cttgttgttc gatatgcttg aaagtaccat gtgatcctac cagtagaact
     2521 gatttgcttc ttgaatcgat ctgttttttc catttattga ttcctcctat tttgaaggaa
     2581 cccaacaagc agaaaccctt tggaaatctt tctatctatg tcatcattgt accgcatctt
     2641 agaaagaaaa tgtaacttat aaagatcgaa gtagtcgcaa tttttctcga tagtcattcg
     2701 aatcactctt tctcatccat tctaaaacat catcacgaaa atagtgtcct cgaatcccgt
     2761 accaacaggg aggaaactca ccaagtttta ctaaacggtt cagtgtacta tcacttatct
     2821 gaaatagtcg ctttaaatca gtatttgtta ataccagagg taaatgactg actgcaagca
     2881 atttttccgt gtaatcttcc agtttttttt gtaaaattga atagaactct atttccagag
     2941 aattaacatt tgttttaact tccaactcca tcacccttct ctctcaaaat caaaacgacc
     3001 accacggtac cccttaaaat tgtaaaaaaa cactccagta ctccatcaaa acggactaga
     3061 aaagaggaat ttttaatatg aaacaaggaa ttgcagacat aaaaataatt aaagaaattc
     3121 ttgaaaaatc tacggctaat gcaatagcat tcggaacagg aatcaacttg agtacagtaa
     3181 aaaaacttaa aagtggagag agagctgaag aaaaattaaa tcttgccgat gctattaaaa
     3241 tcacagaatt tggcatgaaa aatatgccaa ccaaaatcga aatatggaaa tagtagtttt
     3301 tctgatctat cagctgtttt gccaaagcac ataggaatct cacctccccc cagtaatggc
     3361 gggccgattt ggttacggaa gtatcattat cgtagtcgcg attctcatat taaaatttgt
     3421 cgcttaaaag ctaaccttgt catggcgtaa ctagagaatc aaaatctaca tgtactgata
     3481 gatttgctat tcatttgtca aagagcgcta ggctattttc agcctatgga aatcctttat
     3541 tttgacccga taaaaaactt ccatagaatg aaaaggaaag ggaaaaaata ctttaacgag
     3601 ttgtatcaaa ttcaagtatc ttctcaatca attttgtttc cagttgtcta cgcatatatt
     3661 catctacata cataaccatt cctttttctt cttcataaac tggtttcatg gctaaccgtg
     3721 aaatataatt ttgatatttt gacagaacga tggacatggc aattgaatca ccagaagctg
     3781 cggctttgat tgtcgttagt ggtatgaatg gtatatatcg tttattttgc ttcatatttg
     3841 acacccaaat actttcctag ctcttccaaa gccatttgtc gttggtacca aacactgccg
     3901 agacttttcc caataatatc gccaatttca cgatcattaa gaccgatgaa gaaggacata
     3961 aaaatgattt cttgtctttg actaggtaat gctgcaatcg catttgccaa ctcctcattg
     4021 gaaataaaga ttttcatacc aaggacaata aaagtaccac tactattcaa atttggttcc
     4081 aaataataga actctgtacc taaccctttt tgttccaata tttccattga tatttgccgt
     4141 tggcgaaatc tagcgtactt cttttgaata tttaacgcct cattgcagac tactttctta
     4201 caaaaactgt caaacgtatg ttgaatgtgg gtttcctctg agtcgcccat aatattctcc
     4261 ccctcaaaaa attcattcaa ctcgttttac ttctatccct ttccattatt actacaatct
     4321 agga
//
LOCUS       DAYYHX010000197         4247 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000197 DAYYHX010000000
VERSION     DAYYHX010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.199"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..454
                     /locus_tag="ACHA6Y_002473"
                     /pseudo
     CDS             1..454
                     /locus_tag="ACHA6Y_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(580..1659)
                     /locus_tag="ACHA6Y_002474"
     CDS             complement(580..1659)
                     /locus_tag="ACHA6Y_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8988536.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(1762..2202)
                     /locus_tag="ACHA6Y_002475"
     CDS             complement(1762..2202)
                     /locus_tag="ACHA6Y_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8988537.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2259..2669)
                     /locus_tag="ACHA6Y_002476"
     CDS             complement(2259..2669)
                     /locus_tag="ACHA6Y_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8988538.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(2849..3283)
                     /locus_tag="ACHA6Y_002477"
     CDS             complement(2849..3283)
                     /locus_tag="ACHA6Y_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8988539.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(3383..4231)
                     /locus_tag="ACHA6Y_002478"
     CDS             complement(3383..4231)
                     /locus_tag="ACHA6Y_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8988540.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1533 a          799 c          707 g         1208 t
ORIGIN      
        1 atacggattc tggaaaccaa acagtaccgg aaacaaatcc agacacagat aatgaaactg
       61 aaaatccaga aaaaccagag gtagatccag aagaaaaacc agatgtgact ccagaaccag
      121 atacggacgc aagagaccaa ggaataccag aaaaaataaa caaaaaaaca attcaagagg
      181 atggaaaaaa agagagtaaa aaatcaaatc tagcaattct aaaaataaat gaagagcagc
      241 tgaataaaaa atcacgaata tttgatagcg cccaatcagc tgaaacctta aaaagtagca
      301 aggatacaac ctttgctgtc ctgaaacgaa aaataaacag ctaccaaaat ccggagagtc
      361 acaaaacaaa gttatactgt ggagcggaat cattctatta agtatagcaa caatgctttc
      421 tgcaaaaaga tttaaacaaa acagatctct ctaacatttg aataatgtct gtgaacgcat
      481 ttttcaaata aaaagaaata tccagaaaat gaccccaaaa taccagattg aaatctgata
      541 tttggggtcg ttcatacttt atctttcttc atatttattt taagggatat ctgtttcttt
      601 tggaagatgg ttcgggatct ttttcacttt caatacatct aaaaagaagg taaagatcgg
      661 aatgccgaca atcaagcccc aaacgccaaa gaaccgttcg ctaacaagta agaccacaaa
      721 tgtataaaaa atcggcagtt ctgttttgct ggacatcaac ttcggattca gcacataaga
      781 ctcaattaga tggacaatca aaatcgtaat caggatataa acaacatctc ggatgcctcc
      841 ttgggaataa gctatgaaag atagaggaat acaagaaatg ataacacctg ctactgggat
      901 cagacttaaa atgaaaatca tgatcgctaa actcagcagc tgatgaaagc caaatacagc
      961 caaggcgatt gtagtgagca acgtatttaa aagagcgaca acaaattgag cttctaatac
     1021 caaaccaaat gtattgacaa acttatcggc aaaatagtaa atatcttgaa agaaccaaga
     1081 aaattcactt tttaaaaaca atcttgagaa tacaattgtt tttttctttt caatcataaa
     1141 aaagaaactt aagatgaatg acatagcaaa cgccactgcc atttttccta tatcctgtaa
     1201 atagccaagc gcaatcgaag cgccattttg aagctgttca aaaaaatcgt ttcgctcaat
     1261 ataagtatgg atatattgta aaacaggatc tgcatctttt ggctgatgct cgtaaaaatt
     1321 aataacggac tggatcatgt caaatgtttg atgaataagc atcgggatat actttgtgaa
     1381 tgccagatag ataaggtaaa cgatcagtgt ataaatcatg atagctaaaa cacctgtagg
     1441 gattcggaca aatcgctgaa tgatttgaac aagatgaatc atcaaataag taaagataaa
     1501 tgtgagcaaa atggttgtaa tcatacttct aactaagaaa agaacaaaaa cgacaaataa
     1561 taaaacagtg agacggcgta ttttttcatt cgccaaaaaa cgctgataaa cggtcaaatc
     1621 gacaacctcc aaataacaaa aaataaacac gttattcatt atatgatgga atatgaaaat
     1681 aagaaagagc aaaaagaaaa accgctaaat gacgttatct tatgagttgg ttttcctcat
     1741 aaaatcgttc atctagcagt ttcagccctt tttctggtct tgatgaaaat tatctagcat
     1801 ttttcgggtc agatgttgca gtgtatccaa ttcttcctgt tcaagtggaa gataagactt
     1861 gatcttttgt ggaatacctt ctgcttgttt ttgcaacgca gcaccttgag cagtcagcgt
     1921 aacatttacg attctttcat cacccgtttc acgctgacga ttaatataat ttttttctgc
     1981 taactttttt agtaagggag tcagcgtacc agaatccaga cacaaatcat ttcctagctc
     2041 tttcactgaa gtctttttct tttcccataa aactaacatc acaagatact gggtataggt
     2101 aagattcagc ggcttgagga ggggagtgta gtgtctaatg atttctttag aggcggcata
     2161 taaaggaaaa catacttgat taactaaata tagatcttcc atttttccac ctccgctgtt
     2221 ttactatagt gtatcatagc ggatttacac aaaaaagtct attcactaac tgctttcaga
     2281 gtaacttcta tatttccagc tgtagctttt gaatacggac acactttatg cgctgcctct
     2341 aataattctt gcgtttcttc cagagaaaga ccttcgatag atccttcaat ctctacacct
     2401 aagacaaagc tcgtttcact ttctgaataa agggaaactt gtgcactgac agtagaagcc
     2461 ccagagatat ttttttgctg tttgatcaat tctaatgcac tgttaaaaca tgcactgaaa
     2521 ccagctgcaa acaattgttc agggttggtt gcattttcga cgcgtttacc aggagctgca
     2581 atattcaatt cgaatgtacg atcgggatta tgcacttctc cagagcgtcc gcctgtgttg
     2641 atcattttcg tagaatacat ttttttcata aaattaccac ctttaattat attttataca
     2701 attgaatttt acacaatata attgtggaaa gcaaagaaat ggattggcat aagataaata
     2761 agagaaagca catgaaacag acgcgctttc tcgaatagca aacatataca aaaaatatca
     2821 gagacaaaaa ataatctcat cataaacatt acatatattt ctcaaaaagt tgttttcttt
     2881 cttcaaaaat tacttcacga tggacctgat gaagcatcca taaataacca aaaggatctc
     2941 tgaacattga gttagtcaca ccaaagtcca tcatttctgt catttcttga atctctacac
     3001 agccatttcg taatgcttta ttatgtgtgt cctcgatatc agagacgatg atattgaacc
     3061 agatcgtatt tggatgttca gcagtaggag cagtcaaacc aagagacgtg ttttcatcca
     3121 gcatatggaa tctcgtacca tacaaggtaa aaatgacttc attattgcct ctagggtaat
     3181 ctgttgcctc aatcagttgt atttcatcaa agattgtttg atacagttct agtgctttca
     3241 aactatcatt taatacaaaa tcgatttccg ttcctttatg cataaagatc tcctctcctt
     3301 ttcagtcgaa cagttactag attcttttat tataccagct aaagaaaacg cattcaaaaa
     3361 aatagcggat ttacttacca actcattcga ttggatgata agtaaatccg cgtcccatta
     3421 cttctgctac gctgctgaca atgacaaagg cacttggatc gatttcttct atcaatggtt
     3481 tcattcggga aaattcatga tcagtcacaa cgatcaataa aacctctttt gactttcgat
     3541 tatacccgcc ttttgcctct agcagggtaa ggccacgtcc aatctcagca cttaagcgaa
     3601 ggcggatgtc ttccaaataa tgttcactca tgaccataac agatttcttt cgattgatac
     3661 ctgtttcgat ataagtcatg acaattgaag tgattgcaat agataaaata gcataaagaa
     3721 actcttctat tccaaaaata aacagattaa gagaaacaac gaatgcgtct gtaaaaaata
     3781 agccaatcga agtattcaag tggaaatatt ttttaaaaat gaggggaggg atcgtcgtac
     3841 cgccgctaga agagttgttt ttatataaaa tcgcaactcc taaagcaaaa atcgcacttc
     3901 caaaaatgac agacaacaac cgatctgaag taatcattgt ttctggaact attgcaatga
     3961 ttaaaggaaa aaccaagctt ccgaaaagta ctttaaaaaa gggtttttta cctaaaaata
     4021 gaagagctag taccaacata acgatattca acactaaaat aactatagct cggtcaaagc
     4081 ctaaagcagc ttccagtaat attcctagac cgctgacacc tccagcagca atatgatgag
     4141 gccctaaaaa catattgata gcgactccta atatgaagag ggcaaaaata atcttaatgt
     4201 atgtccatat aaattgtctc gtttctttca tttattcact tccttaa
//
LOCUS       DAYYHX010000198         4184 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000198 DAYYHX010000000
VERSION     DAYYHX010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.200"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..415
                     /locus_tag="ACHA6Y_002479"
     CDS             <1..415
                     /locus_tag="ACHA6Y_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988541.1"
                     /translation="LFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSVLVLGTLII
                     AGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFLCFYLPPKI
                     FTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            510..1394
                     /locus_tag="ACHA6Y_002480"
     CDS             510..1394
                     /locus_tag="ACHA6Y_002480"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8988542.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1616..>4184
                     /locus_tag="ACHA6Y_002481"
     CDS             1616..>4184
                     /locus_tag="ACHA6Y_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM8988543.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIPE
                     "
BASE COUNT         1364 a          708 c          947 g         1165 t
ORIGIN      
        1 tttatttcta gaagttattc acgtatacgg atttgttttt ctcacaaata aaaaggcagg
       61 aagtacaatg gatcatttgt atataaaata tattcatcaa ttttccgttt tggtattagg
      121 cacactaatt atagcgggga ttgttgcatt tattattcaa ggtcgtgaca tattaacagt
      181 acatcaagag tttttcagtt atgcatttgg tattcctctg atcgctataa ggtctgataa
      241 caaaaagtgg aagaaacgct attacattct cttgctattt ttgtgttttt atctgccacc
      301 taagattttt actttattag gtatacaatg cctatcattt tcttttattt cctatatcat
      361 tgttttaatt ttcctcagtt tcatagtatg ggtattttat cttgtcaaaa aataaatgaa
      421 tgtttcatga acagtacttc atcacagcct aatttttttc tttatgctat cgttatatta
      481 aagaaatcaa tcagaaggga aagaaagtga tgaaaaaagt tttattagtg gtaaacccca
      541 gttcaggcgg tgaacaagca aaggaatttg agcaattagc gatagcaaaa ttggaatcgg
      601 tgtttgatga agtggtcgtc ttgcatacaa aaaaagcagg ggatgcaaaa aactttactc
      661 gcgaagcggc tacggaaggg tatcatagtg tgtttgtcat gggcggagat ggaacagtca
      721 acgaaggaat cagtggaatc gctgaacaag aacatcggcc aaatttcgga tttttcccat
      781 tgggtacggt gaatgacttg gctcgagcat tagggatacc gctagaacct gaggaagcga
      841 tcaatcattt ttcgattgaa tcagtcaagc cattggatat tggaaaaata aatgacgact
      901 attttatgaa tgtcgtcgca attggctcta tccctgaagc aatcaatgat gtagatgcgg
      961 agaagaagac gaaatttgga aaactagctt atttcatgtc aggaatcaaa cagttagctt
     1021 ccacacaatc ctactcgttt cacgtagaag ttgatggaaa gaaggaagaa attgagagca
     1081 gtatcttgct gataggacta actaactcag taggcggttt tgaaacatta cttccaaatg
     1141 caaaagtgga tgatggtaag ctccatttgg tttatttaaa agattcttct ttgatcgata
     1201 cgcttaaaac attgccagac ctattaaaag gagtagatga atctacgaaa aatctagcct
     1261 atcttacttg tgagcaatta gttgtgtcat tagctgatga ggaagaattg acgacgaatg
     1321 ttgatggaga tgaaggggat aaattacctg tgacaatcaa agtgttaccc tcacatttac
     1381 aggtttattg ttaaaatgga ggttgtgacc gaagagttgt caggaggaca tcggttgttc
     1441 ggaataaggg actgtgacaa gacttttgtc acagcctttt tttgtacaaa aaagaaattt
     1501 tcacctaaca gaaaaatttg ccataaaaat acatatttta aatatgaaag tacaattaca
     1561 aataaattaa aacgttaaaa taaaattgtc aaaacgtcag agaggatggt ggagaatgaa
     1621 tcaatatgta aaaaagctga ttgcgtcgat tttgtttgtc ttccctctct tgatggtgac
     1681 atccgttttt gccgatgtag agaaaacaag cgaatcaacg caatcagaga atcaaagttt
     1741 tgacaagact actttttcag aagaatcaat tacttctgaa actacagaat cccaaagtga
     1801 ggcgcctact tctaatacaa ggcaagaaga aaataagaca agtgagacct cagaagtagc
     1861 tgaacaaacg tctgatagca gtgaagaggt acaggttaga gttgtttcag cggatgggta
     1921 cagcgaagct gccacaacag aagaactgac gcagcttctc gcagacgaat cagtagctaa
     1981 gattcgttta actcagcctt tgactttaga cagggaattg gagatcaagc gagatatcgt
     2041 tatcgatttt ggcggacttg ctcataattt cggcacacat cacatctata tcaatgagac
     2101 acctcagtcg atcgagtttc agaattttaa aggaacggca gctcatccag gtgggttgat
     2161 accaaatatc gatggaaatg ccattatcat tgcttatatg tccgattttt ggggaaacag
     2221 atatcatttt acgggagaaa taaaatttac cgggacactt gacttatatg atggttctaa
     2281 attaggcctg atttacgccc ctcgtgcaac tgtcacatta gatggtgttt ccggtgtatt
     2341 ggatgtccaa ccagtgaaag aagggatgaa tgccgctccg ggtaaagctt atttttgtag
     2401 aagctatcag ctaaatgtaa tcaatggtag tcagctttat ggaccatatc taggaaaatt
     2461 ctatggtttt ctagattcag gtgatgagac gggcagtgga aaaacagcac caggactcca
     2521 tatcttgagt ggttcaaagg tctctcttga ctatgatcgg gcagatggcg tcagcgatga
     2581 aggagaggct gtcgatacgc tacccgggaa tgtcacattt aaagtcagtg gctcaggaag
     2641 tgaattttcg gtaaatacaa agatcaatat taccaacgac aacaatcgcg gaattatcca
     2701 aatgagaggc tcaggatcaa gagtcgctgt gtcgaatgat gggaaaatca caatcaatac
     2761 agcgactact ggcggatttc gcctgcaagg agattctagt caaatccatg tatcaagtgg
     2821 tggacaaatc tcggtcaaaa tggctggaga tggcgaccaa cctggaaaca atgggatgcg
     2881 ttttgtaggg aaaggacttt ctttgatcgt agaaggagcc ggatcagtca tcgatattga
     2941 taagcaaagt ggtcgttcta gcgcagttcg ttttgagaac ggtacgcaaa aactaacagt
     3001 aactaatgga ggctcattga aggtaagaaa tgcaggggat ggcaaatctt atgtgaacag
     3061 actcaatcaa ggaaaccaag cgatccaatt ttatgacgct tctggttttc tagaaagtcc
     3121 agggtcagct gattttacgg ttactggaga aaaatcaaat atcgatatac gcgcagattc
     3181 cggagctacc gtagatacaa ctggagatat tgatttgaac tttatggctg gcgaaaaaac
     3241 ttatttagtc atggtcggga aaactgggga agataagact ggcatcatca gtggggatgt
     3301 gttgaatgta gacttagaat ctcctaccta tttcgatttc caaaacaaac ggatcggaag
     3361 cgagactaat ggcataggtg gctgggtatt tcaaggaaat ggcgattctt ctttgaagat
     3421 cgctcattca gaaattgctt tatggcgaaa ggcaggagta acgttcgata actttgaaaa
     3481 agatccggat tatttttctg atatgacaga cttatcgatc aatggaaccg acttaggtaa
     3541 ttttgtatca agccctagtg caggtttaac aaatgaattt gctcagacag gtgccggaat
     3601 gaaaaattat aatcgaatca gtgccaataa ccagcttccg atgattgaat ccttaagagt
     3661 accaacgaat gcagataaaa aaatctatgg gcatgtcgtt gttcccgaag gtgtcgaggc
     3721 aaagccaaga gatgcttgga caggcgaagt agaagtgact ttacgtatta cttatgcaga
     3781 tacgacgaaa tcacctgtag aaattactgc tctcacacaa ggacaaacag atgaaaatga
     3841 agggacagga tataatccgt ggggagaagg cgaacaaggc ggtttgtttg aggttcaagt
     3901 acccaacgaa gaaagactaa caacggggga tcaagtgcaa atagttgctg ctaaaaaagt
     3961 aagaggcggc caagaagtga cagaactgga ctctgccaaa acgacagtcg atgtgattcc
     4021 tcctgagcct gctaaattaa gtaccacatt gatcaatgca gcttctcgct caatcacagg
     4081 tacaggcgaa gcaggagctt tggtttcatt gaaaaaaaat caagctgttt tgtccgaaac
     4141 aactattgat gatacaggta aatttgttct ttctatccca gaaa
//
LOCUS       DAYYHX010000199         4171 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000199 DAYYHX010000000
VERSION     DAYYHX010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.201"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..600)
                     /locus_tag="ACHA6Y_002482"
     CDS             complement(<1..600)
                     /locus_tag="ACHA6Y_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8988544.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSIC"
     gene            complement(720..1367)
                     /locus_tag="ACHA6Y_002483"
     CDS             complement(720..1367)
                     /locus_tag="ACHA6Y_002483"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM8988545.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1451..2878)
                     /gene="uxaC"
                     /locus_tag="ACHA6Y_002484"
     CDS             complement(1451..2878)
                     /gene="uxaC"
                     /locus_tag="ACHA6Y_002484"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8988546.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(2952..4031)
                     /gene="uxuA"
                     /locus_tag="ACHA6Y_002485"
     CDS             complement(2952..4031)
                     /gene="uxuA"
                     /locus_tag="ACHA6Y_002485"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8988547.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
BASE COUNT         1308 a          885 c          562 g         1416 t
ORIGIN      
        1 acatatcgaa aatattcccg caacaattgt aaaagttgat ccaccacgaa ctatttgagc
       61 gcctgaatca tctcttgtat aaataaataa tggtgttaat acaccaatga ccaaacttgc
      121 taaagtagct ccaacatttc ggaatgtcga tagcattgtt ctttcttctg taatagaagt
      181 aatagctgat gccattgatc cataaggaat atttatagat gtgtaaaaaa tactccccca
      241 taatatatac gtaacataca tgtatactac cttaacgctc atagaagctg aaatcataga
      301 agtttgatac attaaaaaac ttgctaaagc gactggacca ctcattctta aaatccacgg
      361 tttaaactta ccctgcttag ttgctttcga tctatcaaca atacgcccca tagttacatc
      421 agtaactgca tcgataaatc ttgctactaa aaataatgtt ccaacggcag ctccgcttat
      481 ccccaatact tttgtataaa agatcattaa aaaagaacta gcaaaaatga aagtaaaatc
      541 gtttccaaaa tctccaaaca tatatcctat tttgtctctt ataccaaatt ttctttccat
      601 acaattctct cccttctgtt gttaagcgtt ttcattctga tgaaagtacg tactatactc
      661 tgaagcgtaa agagtcagta tttatttttt acatttcaga gtattatttg ttttttctcc
      721 taataattct ttatctcata aaacttatct atatatttac tagctactcg acttacttct
      781 tcaaaatcac ctttttcaac agaagccaat aaattcccac ctacaccaac tgcaacaact
      841 cctacttcaa accaatcttc taagttctct atacttactc cgccagtagg cattatattt
      901 acgtgtggta atggagcttg ataggctttt acaatggatg gaccaaaagc agaacctggg
      961 aatagtttaa tgatatctac gccagcagta agtgcctcct ttatctcagt aattgtcata
     1021 caacctggta aataaggaat ttgatataaa ttacaaatct cagctgtttg tcgatcaaaa
     1081 gatggactaa cgatatattc tgccccagcc ataattgcga tacgagcagt aaacgcatct
     1141 aaaaccgtac cggctccgat tattacatct ttttgatcag catatttttt agcaatttct
     1201 ttgattattt cttcagcatt aggaactgta aaagttagtt caatcccttt aattccaccc
     1261 gcaataattg aatttattgc tttaatggcc tcatccttat ttttcccacg tacaaccgca
     1321 atgactccag ccgcttctaa cttacttaaa atttctactt ttttcatatc tatctccttc
     1381 ctttatggat acatactttg tctagctgta ctaacttaca aagaataaat ttaagctttt
     1441 atttcaagta ttattttcta cttccgattt caaaaaaatt aaaataatta tacgcattac
     1501 gataacaaat attctccact atctgactaa gatacatttc atcatctgga attcggcctg
     1561 ctttaaccca ctcgcttagt aattgacata atactcgacg aaaatactca tggcgaggat
     1621 agcttagaaa acttcgagaa tcagtcaaca tacctgtaaa attcgacaac aaactttgtt
     1681 gtgccatcgt agtcaactgt tgacgcattc cttcagctgt atcattgaac caccaagcac
     1741 atcccaattg gattttttga atcatacccc cttggaagct ttgcattgct gttgctagtg
     1801 gaagccaatc agttggattt aatgaatata aaattgtttt tggtaagcac ccactctttt
     1861 cagcggtatc gaacaatgct acaacatttt gtgtcaaact agcttgatcc cccatcgaat
     1921 caccaccgac atctactcca atttgttcct ttaatatact tgaattattt cttaaagcat
     1981 ttaaatgcat ttgcatcgtc caaccatatc ttgtatactc agacatcaaa tgtaattgaa
     2041 tacctgtaac aaattgtgca atttctgata aatggaaatc cgtttttcct gctaaagctt
     2101 ttattaacag attgtcgagt attttttcat ctgctatttc ataaaaataa gtattcaaac
     2161 cgtgatctgc taatcgacag ccaatttgat gaaaatgatc cactcgttgg ctcaatgctt
     2221 gcttcaaccc attaaagtct ttgattggta tagccgctgt ttgactaagt tttaaaatat
     2281 agttcgaaaa gcctgatgat ttgatactcc agaccttatc tggacggaaa gtaggtaata
     2341 ccttgaaaga attatcctgt ttagaaagct cagtatgata ccacaagtca tctgtaggat
     2401 catccgttgt acagatggct ttcacatcca tcatctccaa caattctttc gggcgatagg
     2461 aatccgtttg aattttttcg ttagcacgtc gccaaatttc tggtgcattc ttctcagaaa
     2521 ttgtcaaatc aatatcaaag taacgttgta attccaaatg agaccattca aaaataggat
     2581 tacctggcgc cttctccact gtttttacaa aagctaaaaa tttttcataa tcatcacctg
     2641 gaccactgat caactcttct tctataccat tagctcgcat caatcgccat ttataatgat
     2701 ctccagctcc tttatcattt aaccatacct gagtcaaatt cttgtattgc ttattttcta
     2761 gcaaatatgc tgggtctaaa tgacaatgat agtcgataat tggttggtgt tttgcaaatt
     2821 tatgaaaaag tcttttagct atttcattct ctaataaaaa gttcttatct aaaaacatat
     2881 ttaaacactc ctttccttcc gttagttttt agaagagaaa atttttcctc taaataaact
     2941 cccttatata attatttact cgcccccttt tcaagcgctt cccaaatacc atttaaataa
     3001 actgcaccta gagctcggtc atataatcca taaccaggtc taccggtttc tccccaaatc
     3061 atccgaccat gatctggacg aatatagcca ttaaatccat tatcgtacat tgtttttaat
     3121 atcttataca tatctagaga accatattct gatttatgcg gcgcttcgta aaaatcgagt
     3181 ccattgtcac ttaaatattt gatattacga acatgtgcaa aaggaatccg atcccgttga
     3241 caaaattcag atagaatttc atagacatca ttatctggtc gttctgcaat acttccagta
     3301 cataatgtaa taccattaga aggagaatcc actacattac acaaccaatc taaatcgtca
     3361 cgatttttaa caatacgagg taatccgaaa atggaatatg gtggatcatc tgggtgaact
     3421 gccattttaa tcccaacttc ttcacatgtc gggataattg ctttgagaaa atatgcaaag
     3481 ttctctcgta atttctcttc atcaacttct ttataggctt ctaataattg cttaatttgt
     3541 gataaccgat gaggttccca acccggcaat tcaaaattat tagtagaatt ttctacttct
     3601 ctaataattt cctccggatc agtagggata ttttttgaaa tataggctaa cgtagaagac
     3661 ccatcatcta aacgataatc caaatccgat tttacccaat caaatactgg cataaaatta
     3721 taacagataa cctcaatccc aaattctgcc aaattgcgaa tcgtctgttt ataattctca
     3781 atgtattgat ctcgtgttgg taaaccaatt ttgatatcat catgaacatt aacactctca
     3841 ataactttca aggtaagtcc attggcttcg acttcatctt ttaaagattt aattcgttct
     3901 ctcggccaaa cctctccggc tggtatatcc attaatgttc caacaattcc tttcattgct
     3961 gggatctgac gaatttcttg tagtgagatt ttttcttcat ttgccccata ccatctaaaa
     4021 gtcatttcca tagttacttt cctccaactt ctgttatata tttattgatt gttttacgaa
     4081 ctgcttgagg acctactagc atttcgcaaa agattttttc agttttcttt cctaccccaa
     4141 cttgatataa atctaaacca aagatttctt t
//
LOCUS       DAYYHX010000200         4168 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000200 DAYYHX010000000
VERSION     DAYYHX010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.202"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            433..1401
                     /locus_tag="ACHA6Y_002486"
     CDS             433..1401
                     /locus_tag="ACHA6Y_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8988548.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            1469..2227
                     /locus_tag="ACHA6Y_002487"
     CDS             1469..2227
                     /locus_tag="ACHA6Y_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFM8988549.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKKIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2295..3005
                     /locus_tag="ACHA6Y_002488"
     CDS             2295..3005
                     /locus_tag="ACHA6Y_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8988550.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3041..>4168
                     /locus_tag="ACHA6Y_002489"
     CDS             3041..>4168
                     /locus_tag="ACHA6Y_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM8988551.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1394 a          628 c          887 g         1259 t
ORIGIN      
        1 agaccataaa ttgcgtacta aaagaaattc tgcaattcaa aataaagcat tttaagccat
       61 tttcaaattt aagtgcaccc atctacccgt caaatcactt tattttccac tccagtagtt
      121 tcctaattca actgtgtgca aaacgatttc ttctaaacaa atcaagacaa acttataaac
      181 cttcatccca gcaccataat tatttaaatc ataggcattt ttcgaaatta gtatatgtac
      241 tcaagtttca ctgcagatta tttatatttt ttagagaaat aaatttttcc tattgacaaa
      301 cacaaagaaa gcggatacaa tacaattatg tcacatgacg ttatatgacg ttatgtaata
      361 tctttacatt taatcagtta cttttatttt ggataaaatt aacattttta aaattttaag
      421 gaggaaatgt tgatggatgt agcaaaaacg gttaacgcaa ttgtagaaaa aaatgatgga
      481 gagatttctg gaatttattt ggtggcttgt ggcggttcgc tggtagatat gtatgtctca
      541 gattattttc ttaaagccga agcatcgaag tgtattacag gcttatatac agcgaacgaa
      601 tttaaccatg taaccccaaa gcaattagat gataaatctg ttgtcattct atgttctcat
      661 ggaggaaata cagcagaaac ggtaaaagca ggagaagtcg ctcaagctag aggagctgtg
      721 actgttggtc tgactcataa taaaactgcg gatttattaa aggtttctga tcatagtttc
      781 ctttacgaat ggggagatga tgcaaaaata gtcaataatc ctatggccat cattcttgat
      841 ttaactgtat ctttggtcaa tgttaccgaa acatttgaac attacggtaa attcagagaa
      901 ggcatgaagg ttatcgatac cgtaattgaa aatggcatta agcaggtgca ggatcgtatt
      961 aaagtgtttg ctgataagta caaagatgaa acaatgtttt atatcttagg tagtggacca
     1021 tcatatggac atgcttatgg tttttcaatt tgctctttga tggaaatgca atggcttgat
     1081 tcaacttctg ttcattctgg agaattcttc catggacctt ttgaagtaac agacaaagat
     1141 acgaatttta ttctcttgat gagcattgga cgcactcgtg ctttagatga acgggcaaaa
     1201 cgattccttg ataaatatgc agaaagagta gaaatcgttg atgcaaagga actaggattg
     1261 gatatgatat cagatgaagt ttctgaattc ttcaatccca ttttattcta cagtattctt
     1321 tcagaatatc gttcagcttt agcagatgca cgcaatcatg atttagatgt tcgtcgctat
     1381 atggggaaag tggagtacta ggaggattaa aatgaaggta ttaggcttag gtgataacgt
     1441 cgtcgataaa tatgaaaatc tgggaattat gtatcctgga ggaaattcat tgaattttgc
     1501 agtttttgca aaaaaactgg gacatgaaag tagctttatg ggagtttttg gctcagatga
     1561 ggaggcagct catgttttat cagcaataaa aaaaatagga ctggacaaca gtcactctcg
     1621 ctttgaaaaa ggcgagaatg gctgtgccag agtgaagatt gatgagggcg accggatttt
     1681 ccttggcagc aattcgggag gtgttactcg tgaatacccg attcagctta ctgaagaaga
     1741 cagagaatat ttaaaccagt ttgaattgat tcatatggga ttatatagcc atgtcaatca
     1801 tctattagaa gagttacaaa atgtttctgg aaagatttct tatgattttt ctgacgactt
     1861 tacggaagaa gaggttcagc gggcaattga taaagttgat tttggcttct tttctataga
     1921 agacttttcc gatgaagaag taaagcagtt tttgatgaag catttttgtg agcgaaatga
     1981 tgtattgatt gcaacccgag gagaaaaaac ggctattggt tatgatggag caaagtttta
     2041 tgaagtgaac cccgtgctag aagaaccagt tgatacaatg gcagcgggag attctttctt
     2101 aacagctttc ctactttatt ttctcgaagg ggacgatata gtcaccgcga tgaaaaaggg
     2161 gaatgaattt gctgggaagt cttgtctggt tgacggttcg ttcggctttg gtattcatta
     2221 cgattgagct tgttttaaat tcctgcattt gataacctaa agaaagaaat cccggtaaag
     2281 tggaggttaa aaaaatgctg gatacaaatg cacaattacc attgtactta caattaaaag
     2341 aaatattaaa aaataaaatc aatacagggg agtatcccga gaatacgaaa attcccgctg
     2401 aatcagagct gatagaaagt tatgaggtca gccgaatcac agtgagaaaa gcagtagaag
     2461 aactggtaga agaagggtac ctacttaaaa aacaagggaa aggaactttt gtcaaccggc
     2521 ataaagtttt tcgtaaaatt gaatatgtta tgggtttcag tgagagttgt aaatctaatg
     2581 gttttacacc gactagcatt gttttggaac gcaagatcat tccagcaact gaagggatgg
     2641 caaaaaaact cgatatcgct caaggtgatg atgttttata tatccagaga aagcgaatgg
     2701 cggataacat ccccattatg ttagaaaaca attatttttc ttatgaaaga tatagtttct
     2761 taatggagga agagctgact gattctttgt atgaagtact taaagtccaa ggtaccttag
     2821 ccatcaatcc tggggaaact gcacttgaaa ttgtcacagc tgatgaccaa attgccaaaa
     2881 taatggaagt agctgttggg acgccctttt tctttatgga tactttaatc aacgatcaaa
     2941 acaacatacc aattcatgta ggtaatcaat attacctggg agaagtatat cgtttttact
     3001 tatgatttcc tgttttgatt tcaaggagta gattttattt atgaagcaag tattgcggtc
     3061 aaaaatgatt tttgatggag agcagccacc tttcaaaggt tatattgtaa tcaatgaagg
     3121 gatcattgaa aaggtggaaa acaactggaa ttacagtgat ctattaaatg aaaacacgga
     3181 attaattgct tatgatgatc aattcgttat gccagcaatt catgataatc atgtattttt
     3241 cagtggttat ttggctatga atgctggcat agatttaagc gatacatctt cagctaacga
     3301 ggctgtagag cgtattaaag ctgtaatgaa ccataaaaaa gcagatgaac caatttatgc
     3361 tcatggctgg gatgctgccc tgtggaaaac tgacccatca gaagagctat tgaatgaaat
     3421 aaaccacttt ggtccaatca cagcgatcga caaaaatcgc agccattgtt ggatgaatgc
     3481 tattgctaag cagacttatg gttttacaga aaaagaaacc agtgctgaaa gtcgagtatt
     3541 attaattaag gaaatgctag caaacaaaaa gcttttagca gaggtttacg ctgaatttga
     3601 agcattactg ctttcaaaag gtgttatatc aatgaaagaa atcgtttttg atgacgcaga
     3661 atttctttct aatatacctc agaggaaaat acttagtaac ctatatgtgc aggcagtcca
     3721 agaacctcta cagcatgaac ttctatatga gtataaggaa aaagttttcc ctgaaaaggt
     3781 ccgctttggt ggagtgaaga tcatggtaga tggtgttgtt gcggatagaa gtggagatat
     3841 ttacggtgtt tatcagtcag gagtagcttc acccgagatt gactatgagg caattaaagc
     3901 agaagttgct gcattcaatg aagtagagat tccttgctgt ttgacaacgg aaggagataa
     3961 agcgggttat aaagctgcgt caattcttgt cgaatttgga aagcggttac ctgaaggcgt
     4021 ttttaattcg attagtgatt tggaaatggt tactcaaaag acagcagaaa aaatgaacga
     4081 aggacaagta gtagcagagg tatatccaca aattctcggc ttgaatcaaa cgttagaaga
     4141 ggcttacatg tcaactgtta taaaaaat
//
LOCUS       DAYYHX010000201         4163 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000201 DAYYHX010000000
VERSION     DAYYHX010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.203"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..546
                     /locus_tag="ACHA6Y_002490"
     CDS             175..546
                     /locus_tag="ACHA6Y_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988552.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            539..934
                     /locus_tag="ACHA6Y_002491"
     CDS             539..934
                     /locus_tag="ACHA6Y_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFM8988553.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            936..1313
                     /locus_tag="ACHA6Y_002492"
     CDS             936..1313
                     /locus_tag="ACHA6Y_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFM8988554.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            1314..1922
                     /locus_tag="ACHA6Y_002493"
     CDS             1314..1922
                     /locus_tag="ACHA6Y_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8988555.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            1922..2263
                     /locus_tag="ACHA6Y_002494"
     CDS             1922..2263
                     /locus_tag="ACHA6Y_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988556.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            2505..>4163
                     /locus_tag="ACHA6Y_002495"
     CDS             2505..>4163
                     /locus_tag="ACHA6Y_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFM8988557.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVE"
BASE COUNT         1477 a          543 c          865 g         1278 t
ORIGIN      
        1 tgaagtgttg tataaaataa taaaaaatga atcagaaaaa acagtatctt cagttagacg
       61 tggcgataca acgattagtt atgcttcaac aacaaatgat gttagtgagt tacttctagg
      121 ctatggtgat ttgatacgaa gagttattgg ttgtggagga ttggagtttt tttaatgaat
      181 gaagcagata ttttagaaac tacttatgaa gatagttgta ttattgaaag actgacggac
      241 attgaagatt ctaatacaaa tattactatt caagattaca aaaaagtata tgataatcct
      301 atttcttgcg ctctttcaca aggtcaaatc gatggactag cagtcataga agatggagag
      361 atggtaaatg tttcaactga cacatataaa ttatttgttc atcctaagat taaactaaaa
      421 aaaggagatc gaataacaat aactcaaaag gccagtggct taattttttc tctatttgct
      481 actaagcctt tttactatcc tagtcattgt gaagtgaatt tgataggaag tgagaaaaat
      541 gggtaatctc aaatatgaat caaatgcaga aaagatcatt gagaatttta aaaatatgac
      601 tgtaattgct caaaaagaag gaatatcctt cgttaacgat tcaatgaata aagtcgttag
      661 tttagctaaa ccattaactc ctgtaaaatc aggtaattta aggcgaggat atcgtgtagt
      721 aaaagctaga aaactatcaa gcggtcgaat tgttggagaa gttataaaca atgaacatta
      781 ttttaaatat gtagaagaag gtcataggac taaaaatggt ggctttgtca aagggaaatt
      841 catgttaact cgtgcaacga atcttgcaaa tatgtcttat attcctcgaa gatttaaaca
      901 aatggcaata aaaatcgtta agaaaggaaa gtaacatgaa agatgaaatc attgctgcaa
      961 tcagtagcaa attaaaagaa atctatcctg atgggacaat atatcttgat tcagttatgc
     1021 agtcaactaa agatttttat ttcgttttat cagtaatgga atctggaact gaaaatgtag
     1081 gaattgatat tcaaaatgtt tctttcttaa ttgatattgc attgattgat aataacccta
     1141 atagaaattt aattaatgaa ttagtctcac gttgtgggac tttttttaat acgattacaa
     1201 tcgatgagca aatattattt ccaaaagcct atttacctga tgaagcagat ggtgttcaac
     1261 atattcgttt tacattagaa tttccacaat atattgaatg gagtgaaaga taaatgggag
     1321 aaaaaagaag taaaaccgga attatttctg tcgaaaaacc tacttggttt cctttggaag
     1381 atgagacagg gaattttccg gtatatggta ctgcgttgcc aatgggaaca gcagtaagta
     1441 tcaaaccaac agctaattat gaaacaacac aagattatgg ggattcagtg gttcaagatc
     1501 aatttacagc ttttggtggt gcagaagttg aactggaagc aaatggatat actcataaag
     1561 ttttatctac aattactgga gggaaaattg ttaaaggtgg tgccttgcgt tctggagaag
     1621 atattgcgca agatggtgcg tttgcatata gacgtaaaaa atcgaatggg aaataccgtt
     1681 atacggtctt ttataaagga cagtttgctc tagattcaga tgaaacatca acgattgaag
     1741 gaagtaaagt aagttttact catccaactt ggaagggttc atttgtagat gtaccaggac
     1801 ttggatatat gtattcagtt gatgaagatg atgaaggcgt ggataaagca atgatcggaa
     1861 attggtttac aaaagtagca attccaattg aagaagcaga actttcagga ggtacagaat
     1921 aatgtcaaaa tatcaaacaa cgattaaatt aacgaaaaaa gacgaagaag gtaaatatga
     1981 gcaagtacaa tttaaatctg ctgaattttt accaggaaca gttgtagaag atgctgcagg
     2041 agttatggaa gaaatgcaaa cagcgactga taagcaatca gttaaaaaag ctttaagtcg
     2101 tgcttattca tttattgcag atactttatt tgagggacaa tttactggtg aagattattg
     2161 caaaggaatt gatgctcgtg agattgcctc attgacagga aaattattga agtctgttac
     2221 tgcaggtttt gatgaaactt atacagagac gaaaaaaaag taagtgaggc gctcaagtca
     2281 ccttcattta agtattcgat tacttaccga gaattagata taaagacgca attactcgaa
     2341 gcaggttgga cgttaccaga aattgaacgt actgacttgg atgagttaat gcgtcttttt
     2401 gcttttagag atgcggtaaa agagcatgaa gatgttgagt actatgataa tttcactcaa
     2461 ttttaggagg tgatactttg aataacgatg acctaattct gaagatgata ctagatgaat
     2521 ctggttttac tgccggtatg aataatgctg tcaaaaagtt gaactctttt gatgaaacaa
     2581 tcgaaagaaa aagtagaaat agtggcagtt cattaggtag tatttggaaa atatttgctg
     2641 gaagttttct agctagcgga gtaactagaa tcgtaggagc tggctttgat ctaatcaagg
     2701 gttccataag tggagcggtt gatcgagtag atacgatgaa taacgctcta cgaaatttcc
     2761 aaaatatggg atttagtaat tcagaaatta tgaagaatat cggaaagaat gggcttttat
     2821 ctcaaggtat tcaaggactt cctactgctt tgaatgatgc gataagtcat gttcaacttc
     2881 ttgcttcttc tacaggtgat ttaactcgtt caactcaaat atttaaagct ttaaatgatg
     2941 gaattcttgg tttcggtggt tcaactgatc aagttaatga agctgttatt caattatctc
     3001 aaagtttctc aaacggaaaa gtagacgcac aaacttggaa ctcaatgatc aacgctcaac
     3061 ttggacctac tctttctgct attgctaaaa agatgggaat tacaatggga gatctgaaag
     3121 aaggtttatc tcaaggtaag atttctgttg aagaattcca aaatcaatta atagaaatgg
     3181 ataccaaagg tggcggagga cttaaatcat taagtcaaat cgctaaagat tctactaagg
     3241 gaattaaaac ctctatacaa aatgcaaaaa cagctgttac acgtggtgtt ggtgaggtaa
     3301 tagaaggatt aaataaagca ttagtagatt cagatttagg tggatttaag ggaattattg
     3361 ataaagtggg cagttcaatg gaatcgttct taaaagtaat tgctgcaaat attcctacag
     3421 cagtatcatt tttaagtaat ttatttaacg aaattcaaaa atttggttct gctttgaaat
     3481 tcatgatgcc ctttcttgtt cctgcagcta ccgcttttgg agcgttcatg tttcaactta
     3541 aaggtatacc agcaataata aaaagcttca ataattttaa gaatgccata attggcgttg
     3601 gaaattcatt aaagattatg ggggcaatag ctgctgctaa tccatttgtt ttaattattg
     3661 gtgcagtcgt tggtgcaata gctgttttcg gttactttat ggcaaccaat gaagagctta
     3721 gaaacaaggt tatatctatt tggaatgatg tgaaagattc cgtaattggt gtattaaaaa
     3781 atataaagga ctggggaatt gatacttgga attctgctaa agaaatggca tcaaatgcag
     3841 ttgagggtgt caaagatgct tggtcaggaa caaaggaatg gttttctaac acctggaaag
     3901 gtattaagga tggggccaca ggtttatttg ataaaacagt agagacttca agaaatgcag
     3961 tcgatagtgt aaaaaatgca tggtctaata tgaagaaatg gttttctgat acttggcaaa
     4021 gtataaaaga ttcagccaga gaaaaatgga atggaatcaa aggttctatt atgtcagttg
     4081 ctggtccatt gattacaggt ataaagaatg catttttaca tgttactttt tatctagaga
     4141 ccttatggaa taatttagtt gag
//
LOCUS       DAYYHX010000202         4081 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000202 DAYYHX010000000
VERSION     DAYYHX010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.204"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..1374
                     /locus_tag="ACHA6Y_002496"
     CDS             73..1374
                     /locus_tag="ACHA6Y_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8988558.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1401..1661
                     /locus_tag="ACHA6Y_002497"
     CDS             1401..1661
                     /locus_tag="ACHA6Y_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8988559.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2660..3895)
                     /locus_tag="ACHA6Y_002498"
     CDS             complement(2660..3895)
                     /locus_tag="ACHA6Y_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8988560.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1161 a          662 c          853 g         1405 t
ORIGIN      
        1 aattagttat gaacattctt tgcgttgtca tgcaatgaat ggttcatatt ttattgaaaa
       61 ggagtggttt tagtggaaaa gctgatgaaa tgggtcgaag agaaacttgt cccaccaatg
      121 gcaaaaattg gtacacagcg tcatttatta gcaattcgta atggggttgt ttcaacattg
      181 tcattgattt tgattgggac attttttatg gtctttatca atttaccgtt tcctggatgg
      241 aatgaattta ttgcccctta ttcggcaacg attgttttac cctttaggat gacgatggga
      301 ttgatggcta tttatgcaac ttttgttatg ggttcggatc tagctaaatc atatggatta
      361 gatagtgtga caggtggtat attatcttta ggaacgttct ttatgttaca agttcctgta
      421 aacgttttaa ctcctgaaga ggcacctctt ggctgggtct tacctatgag ttctttggga
      481 gcatctggga tgtttgcggg gatcttatct atgatttttg cagtggaagt atatcgtttc
      541 tttaaaaatc gaaatattac tatcaaaatg ccagaacaag tgccacctgc tgttgcccgt
      601 agttttgagg cattgattcc aggtgcagtc attttaacaa ttacttggct gattcgaagc
      661 gtcgcgggct ttgatgtaaa cgctgcctta ttatcgttat tcgagccttt aaccaatatt
      721 ttagggaata atttgcttgg tgttttacta ccaatgtttt tgatccattt gttgtggtca
      781 tttggtatcc atggcatgag tattgtaggg gctgttgttc gtccaatgtg gctgatcatg
      841 ttggatgaga atgcaaaggc attggcagat ggtacagctg ccacccaatt accctatatc
      901 gctcctgaac agttttatca atggacggta acaattggtg gtgcaggagc aacgatcgtg
      961 gtatctgttt tgtttatatt cttctgtaaa tcaaaattct tgaaagaggt tggtcgtttt
     1021 tctattattc ctggtatctt caatatcaat gagcccataa tttttggagc tcctatgatg
     1081 ttgaatcctt acatgttcat cccgttcaac ttagttcctt tagttttgac tattatcagt
     1141 tattttgcag tcaaattaga aatggtcaat agatttactg ttttgccaac atggacatta
     1201 ccagctccaa tcggcggtta tctttcagca ggaaatgact ggcgtgttgt tatcttgatc
     1261 atgatcaaca cattgatagc atttattatc tattatccat ttgtaaaagc ttatgataaa
     1321 aagatgctgt cagatgaaca aagtcgtgga gaaaacgaag tccagactgt ctaagtttgg
     1381 tttttagaag gtgggaaaca atgtattggc tgattttatt ttttgttttt atctttttat
     1441 taacggctag tcaccttata ttgaatatgc tagctacata tcatattcaa atcaatcgat
     1501 ggatttgggc actggcatca tttttgatcg tgattcttcc aaagatcatt gtaccgcata
     1561 tgaatgtttt gttttcatgg gggacatatg tactttgtgg aatttttgct atcaatttta
     1621 tgatcgagca gcatagatgg tttgtcacat cgaagcttta aatgttctaa aagtggttgt
     1681 aacagaagtg gttagcttca agaaataaga agaaatttac gaaaattgct tcttaaattt
     1741 ttgtgaaatg ttggcttatt tttcgtttgg ttgaacttat taccgcagat gcaagctttt
     1801 gaccaccgtt tattcggaat atgggactgt gacaaggctt ttgtcacagc ccttttttac
     1861 ataaagatta ttattgtttt aactgttttg gcagaaagaa ggaggaaaaa atggaaattt
     1921 tatcagcaat ggatttagaa ttagtagatc tactaccaaa gtgatcaagt caaatataga
     1981 tttgggagct tacaaccata tagtaggggc tgcagtatgg tgtatgaatg gtaatatgta
     2041 tgatgtgagt gtttattcaa atcatgaaac gtgtatagaa ataattgtgc tgggaatagc
     2101 aatcagtcat ggtgaacgtg acttttagtg tatcgttgct gacgacatag aacattctga
     2161 catgatctac tcaatatgtg gggattgctg gcagtttata taggagtatg catcggattg
     2221 cgagatcatt gtctcaacag aagagggagg ttgattatca aagataaagt ggtaaatcct
     2281 acatttgatt tagtaattgt tgaggggact agcggcacaa atctctacaa agcgatgctt
     2341 ctagatctat atgacacatt ttaagaaaat gtatttgcct aattattatg gtctttcttt
     2401 tgaagaaacg ttgagatgat ccaaccagag aaaggtgtca aagaatttga ctaagaataa
     2461 atggcagacg ctacctatgc tcatttggat agcgtcagat cttttatttt aaggatgatt
     2521 ttgtgttttt tagacaccgt aatttaagta actctaactt ttcaatccac tatttacttt
     2581 ttttgtattt tcactgtctg ggtctcgaat agcgaaatta ctgatgagtc tctattaaaa
     2641 aagctttttc tacttgaaac tatattgggt ggttgctcag gaaggtttcg atttcttgaa
     2701 ttgcttgttg tggaccattt attggaaaaa cggcttgcag cgtgcgtgcc tgctgacgaa
     2761 tcttgggtga agaaacttcg cgcagagcaa tctgcagttt tctttcagtc actggttttg
     2821 tgagggcgat tgcgtttccg tattttacac aatagtcaat attacagctt tgctcaaatt
     2881 gcatccccac accgataaac ggtttgccgg attcacaggc agtttggacg gtcccttccc
     2941 cgccgtggat aaaagaaaaa tcaatgaatt catgcaaaag atgactagac agatattctg
     3001 ttattaaaag atcttctgag tcagattgaa tgtcatcaag cagatagtca ataggtgcaa
     3061 taaccgctag atccggttgt ttttgtaaga agaacaaaat tttgcgtaac atacgcggat
     3121 tgcctgaact acccattgcg aaatagataa tacgttttct ttcttgttgc tttcgctgta
     3181 taaaagttag cacttcttca ggcaaggaag tactgagctt agcaaaaatt ggaccaacga
     3241 ttcgataatt ttccggcagc tgttcttctt ccaaaaatag tttaggcgtt gtgatgagat
     3301 ttagatcagc atccatcatt tgtaaacttg tttttggtaa aggaagcatt tctttttgtg
     3361 cagcgtgttt gaaggcattg gggatccatc gggtatttac tatacgcttt tttgtccagt
     3421 gccaaagggg attgcttaaa gggccgaaga aacgtaactc gtggggcatc agcgcatgct
     3481 ctgattgaaa aaaaggttca ctcaaagcat agggtttaac atatactaaa ggaatctttt
     3541 tattgcgggc cgataaaaaa attgtcacat tcgaacctgt cacaatcagt cgaggctcaa
     3601 acttagcaat cagactctgt tcactagtga tacgagaggc aaccatttct ttggtaaaag
     3661 ggtttttaag tgtacgcaac tggtcaaatt ttatgatttg ttcttcttgt tttttcgtaa
     3721 ggatcggcgc tagtaaatga tactcaaagc cactttctgt gatcaaagga gcaaaagttt
     3781 tagaaaagcc aaagaataca cagtaataac ggtctttcat agctttggca acctcaatca
     3841 ttcgtgttgt ctcagctaaa ttgaatactg caggagcaaa aagaattgtc ttcatgaaat
     3901 cctccttatt ggaatgagtc tatcccttcg tttcttacta gtatactaaa aaacacagcg
     3961 ttcgagtagc ctatgtatat tttgaatatt aaactgtctc tcgctgtttt cacaacttct
     4021 aaatttaaat tttgtaatca agttgaaact ccaattttca aaaaaacacg caacaatttt
     4081 t
//
LOCUS       DAYYHX010000203         4070 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000203 DAYYHX010000000
VERSION     DAYYHX010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.205"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..1435
                     /locus_tag="ACHA6Y_002499"
     CDS             86..1435
                     /locus_tag="ACHA6Y_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8988561.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="ACHA6Y_002500"
     CDS             complement(1563..2153)
                     /locus_tag="ACHA6Y_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8988562.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="ACHA6Y_002501"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="ACHA6Y_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8988563.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1176 a          744 c          789 g         1361 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct
     3841 cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg
     3901 agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc tccatatact
     3961 ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat ttcttttata
     4021 atatagattt tagacatata tatgatataa aaatagtttt cgcaaaaaaa
//
LOCUS       DAYYHX010000204         4056 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000204 DAYYHX010000000
VERSION     DAYYHX010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.206"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..607)
                     /locus_tag="ACHA6Y_002502"
     CDS             complement(65..607)
                     /locus_tag="ACHA6Y_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM8988564.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            742..1131
                     /locus_tag="ACHA6Y_002503"
     CDS             742..1131
                     /locus_tag="ACHA6Y_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988565.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1135..1899
                     /locus_tag="ACHA6Y_002504"
     CDS             1135..1899
                     /locus_tag="ACHA6Y_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8988566.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2028..2975
                     /locus_tag="ACHA6Y_002505"
     CDS             2028..2975
                     /locus_tag="ACHA6Y_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8988567.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3105..3845
                     /locus_tag="ACHA6Y_002506"
     CDS             3105..3845
                     /locus_tag="ACHA6Y_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988568.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1351 a          687 c          815 g         1203 t
ORIGIN      
        1 taaataaaaa ctgtctaaca tttaaaattt aatttagata ttagacagtt tttaagtttg
       61 tgagttattt tccttgtcgt tttctcactt ttttagtcca gaaaccacct gaaatatatt
      121 ttggctcata agaaatgata aaagcacgct cttcgatttc tttgatcagg cgatacagtt
      181 cacgttcgct ttttctagga gaaagtattt cgagaactag ccgctcacct tccagcccca
      241 ttgcagaagt ctgtgtcaca ccataaccgt gttctctcag tatacccggc aaacttttat
      301 cttctgcgct atttgctggc aaaatcgttg tgaccatgat gtatcctaat gccaatttgt
      361 cttcaatcaa aattccgaca ctgataccta ctgcgtaacc taacgcatag acaaataaat
      421 tcagtggatt atctaaacga tctaaaacaa gggataagcc gacgatataa atcgtgatct
      481 cgaccatact gacaagtgga gcaatcagtc gatagccttt cattgtcaac atgaaacgta
      541 acgtgttcag agtgatataa gcaaaattaa tgaggaagat catccccaac atttttggat
      601 caatcatttg taaaactcct ttcatttata taacaacaat atcaaaaaaa atcgtaagtg
      661 aagaaaaatt gaaactttat acatacaaaa aaatgataaa atgattataa tagaattata
      721 aagataggtt aggtgattaa aatgaaaacg gtcaatcgtg tgttttgtgc cagccacgtg
      781 ctaagtctcc ttctcagtat atacttaggt gtctattcga ttcaacagcc aattgaggta
      841 gagaacattg tttttcagat gtcgctgaca gatggtttaa ttttaagcgt ttttttcttt
      901 ttgatactgt ttattggtaa tattttcggt agcgttgcca gttttttgaa tttttctatt
      961 tatccactcc tttcgctagc attaggagtg gtgggacttt gttctctgtg cttatttccc
     1021 gagccattca gtcctgcatt tatattattt ggtacactga acctgttcca gacaactgtt
     1081 ggagcatggt tgctatggcg atcaggaaat ttaatgaaaa taggtgagta acaattgaat
     1141 ccaattatat tgaaacgttc tgtccatcat attccagtgt tagaagttgt tctgaaagaa
     1201 ttaagagagg aacgtattcc cgttgtcatc tattatcacg gatggcagac aagtaaggag
     1261 ttagttttga cgcaaggaag aaaaattgcg cagcagggca ttcgtgtgat cctgccagat
     1321 gcgatgaatc atggggagcg caaacagccg gtttcttcta ttccatccta tacattttgg
     1381 gatagtatct acggaaatct gtttgaattt gacacactta tcaagcattt agaaaaaaga
     1441 ggtcttctac atgatcagct tgcagttggc ggggtttcga tggggggaat gacaacatgc
     1501 gccttgttgg ctaaacatcc tcatatagta gcgggcgtgt gtttgatggg ttcccctgca
     1561 cctttggaat atggaaaaga aatcgtaagg cgcgcatcgg aatataatta tgtcgtacca
     1621 aaagactatt ttgatttgat ttcatgggtt aagagctatg atttgtcatt gcagccggaa
     1681 aagttagctg gacgtcctct gttcttttgg catggacaag ctgacgaaaa aatcccttat
     1741 atacaaacgg aacgatttgt agaaaaaaat cggcataagt cttacggaaa agaaattgtt
     1801 tttcgttcct caaaaacaca gcgacatatg gtacaaatac cactaatgga agaagcttcc
     1861 acttttttag caaaaaattt gataaaaaag tataaataat tgaaacggaa aatcactcat
     1921 gacgatagag gattttccgt tttttatttt gggctataat aaactctgtt aggaaaattt
     1981 ggtataattg atagtaagaa tgacaacatg ttagaaagga agagagcatg cccactttag
     2041 cagatgttgc aaaacgtgcc aatgtttcaa aaatgacggt ctctcgagtc atcaatcatc
     2101 cggaacaggt gactgacgaa ttgaaagagt tagttttctc agcaatggct gaactggatt
     2161 accgtccaaa tattgctgca aaagcacttg tcacgaatcg gtcgcagatc atcaaattat
     2221 ttatactgga agaaatcgac acaactgagc cctattacat gaatttgctg atgggaatcg
     2281 ctaaacggat cggtaaagcg cattattctc tccaattagt tacaaatgat gccttcgacg
     2341 tcggttcttg tgatggatac atcatcacag gtgtcagaaa aaaagacttc gattggatcg
     2401 accgtttgga aaaaccagtt gtcttgtttg gggaaaattc tgaaggatat gattatgtag
     2461 acagcgataa tgaatatggt acagcaaaag caacgcaata tgccttagaa agagattatc
     2521 aaacgattat ttatatcggt atcgaagtgg acgagccttt tgaaatttca agggaaactg
     2581 gttatttatc tgtgatgaag gaagttgatc ttcctagtaa aatcttgcgg tttccaaatc
     2641 attcgacaga agtagaaaga tatatgacag aaaactgggc agattttaca gataaaactt
     2701 gttttgtatg tagttcagat cggctagcac taggagcagt aagagggatt ataaatgctg
     2761 gaggacactt gcccgaagaa tttggtgtga ttggatttga tggggtattt ttagaccaaa
     2821 tcagttcacc taaattgaca acgatcaagc aagatattat acgtatgggt gaagtatgtg
     2881 gagaaatgtt gttaaaaaaa atagaagaaa aaggggcgag tcaaggttat cgtcgttttt
     2941 ttcctgaact agttatcaga gagacaacga attaagtctt attactatat atatatttat
     3001 gaaattcatc gctattttct taaaataatc agcgcattgc tactgaaaac aatgaatctg
     3061 tgctattatt gagaaaagtt ccatttttga gaaggatggt ttgaatgaaa gaaatcagac
     3121 gaaaacattt ccgttttcct gcgtacgatg atgagttagg cgtaaaatta accaacgaca
     3181 agaagcgagt attatttgac ggacaagaag atcttttgac tgaacagaat cagtccccgt
     3241 ttgaacagat gaaagaattt catttcacga acacgaacac cgagaaaatc cgtcgcaagc
     3301 gtcgtgcaac aaatggattg aagtcagctc cgcgtaagga aaatttggaa aagcataaag
     3361 gaaaattacc ggattatacc ggccattacc aatcagaaat cacatcgaca ggacgaaaaa
     3421 aactttttgg tgattcctct cgttctgtca gacaaagtga aacaaggaca gagtccaagc
     3481 atactacacg aaacgcatct gttgacacga aggtgcggga gcgttcttat tttgttccta
     3541 aatatgtacc cgcatcgatt atcccagacc ctagagaaag caaaatttct gaggatcatt
     3601 taatcgaatc gatgaaaaaa acggatgact cttatctgtt gttcgataca gaaccgacgc
     3661 cgtatcaaga aaaaagaaat ggtgagccgt ctgtacgaac attcaatcga acaaaaacgt
     3721 tagaggaaac aaagcagtca gaagaaaaaa aatcgaaaaa aagccatggt atcttagaac
     3781 gctcattaca aggattgatc gatgatcaag gaaactcttt gggagaaaat agctatttta
     3841 aataagtgat atacaaattt atattcggct acagttggaa aggaaacgct gcggcgtttc
     3901 cttattttat actctatccg aaagtttata tcgtatcctc aaattttttt gagtttaagc
     3961 gaattttttc tcccaataag taagaaaata atggcaaaat aaaaaaacaa actattttcg
     4021 cagatcgttt gcaaaaatag tttatttttt tataaa
//
LOCUS       DAYYHX010000205         4055 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000205 DAYYHX010000000
VERSION     DAYYHX010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.207"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..548
                     /locus_tag="ACHA6Y_002507"
     CDS             15..548
                     /locus_tag="ACHA6Y_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988569.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(674..1351)
                     /gene="nth"
                     /locus_tag="ACHA6Y_002508"
     CDS             complement(674..1351)
                     /gene="nth"
                     /locus_tag="ACHA6Y_002508"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFM8988570.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1358..2056)
                     /locus_tag="ACHA6Y_002509"
     CDS             complement(1358..2056)
                     /locus_tag="ACHA6Y_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8988571.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2324..3508
                     /locus_tag="ACHA6Y_002510"
     CDS             2324..3508
                     /locus_tag="ACHA6Y_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM8988572.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3514..3957)
                     /locus_tag="ACHA6Y_002511"
     CDS             complement(3514..3957)
                     /locus_tag="ACHA6Y_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8988573.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1072 a          786 c          658 g         1539 t
ORIGIN      
        1 aaaaaataca atatatggtt ttgaataatt ttttgaaaga aggcttcatt ttgagttatc
       61 tgatagtatg cttaattatc ataatttgtc tgcaccatct ttttttatcg aaaacaatac
      121 ctcgttattc caagaataaa aaagctgaaa aattttgttt attattaaat aaatttactt
      181 taattgctcc tatccttgca tttattattt tttctgtact tttaagcact actcttaaag
      241 gaaaatttat ggaaagaagc tctcatgcaa tgattctcac ttttttatgg ttgttgttca
      301 ccagaatcta tatttttctg atgagcctga aaccgcccaa atccattagt ttatgtttag
      361 ttataaatgg tattttttta ttatcactta tcatttttat tacaccttta gatagatacg
      421 tcacttattt atataatcct ttggaatact ggacttattt tataggtata ttggaaggca
      481 ttattttcta catcgggtat tttcccaata aaaataacaa cttttatttt tatcgcaata
      541 aattgtaata agttaccatc ctagctactg agtatgtaca tttacttttt catgcaaaaa
      601 agataaacga aacagctttt ctccataata acaagataat tcatacattt atttgcaatt
      661 aaagaaacca cgtctacagg cgtggtttct tttttagcgc cttttctttt agacgcatcc
      721 gatttttccc atcttgacat attgatagca aaggacatac ctcgcacttg ggatttcttg
      781 ccgtgcaatg atatctacca aaaaaaatca gtgtatgatg tgtctttacc cataattctt
      841 gtggtacttt ccgcatcagt gtttcttcta cttccatgac cgtagcatct agtttgcaga
      901 tcctcaggcg ttttgagaca cgttccacgt gggtatctac tgcgattgct ggtatcccga
      961 atgcatcccc caatacgaca tttgctgtct ttctgcctac accaggaaga gacatcaatt
     1021 cttctcttga cgttggcact tgcccatcga aacgttcaat cagctgctgt gcacacgctt
     1081 tgatgttttt tgctttattt cgatacaaac caatcgtttt tattttaaga atgatttcat
     1141 cgattgaagc ttctgctaac gcatcgggtg tcggaaaaga ggcaaacaaa tctggcgttg
     1201 ctttattcac tgatacatct gtcgcctggg cacttaagat cactgcaatc aataactcaa
     1261 atggattatt gtgcttgagt tctccatgtg cttcgggaaa cattccatac atcgtttcta
     1321 gagcttccat tgttttttgt ttgcttaaca tattccgcta ctcctccaac caatttttca
     1381 gtgagacttt cggcaattct tcttgttgag ctgactgttc tacttctctt tgaagaagct
     1441 gctgttttct tcgtttttgg tcttcttcca cttgttgttt tgtacgtaga ttttttcttt
     1501 cccacgatag caaaatgcgg tcaacgtaat taaaactata agcctgattt aatacggctt
     1561 cccgcagtgc tagcttcaaa atttccggct gataatggtc ttcttctagc cattgtccga
     1621 tccgctggaa ctcgatcgaa gaaagcgggc gtccaaattc ttgttcaaat aattgataca
     1681 ctgactggat ttctttttct tgtgtttttt cttcgtattt tttttcttgt gtttttaaat
     1741 actcacctaa tagatcatag atggggtaaa gattatatcg atctgctttt ttaccgttga
     1801 caatcgtcgt ttctatcttg ataaatccat ttgttacgag acgatccagt atttgaaaaa
     1861 tctgatcttg tttgattccc atatccatag caatcatctg caaatcaggg aaagtatctc
     1921 cttcaagctg tgccatatgc aactgtagaa caaataaaaa ttcttcagat tgtagaccga
     1981 tcctatgata atttttaagt aaaagattag atatgactgt ttgtccagcg cttaagtaat
     2041 tttctaaatt caacatgatt tctcctccgc tcaatcacag tatacgtaaa aaaaacatgg
     2101 gaagcaagaa caggtttctt ttctatttac ttcactgatc ggtcagattt acaatgtcgt
     2161 ctacttgatt tttgtttgtt catgtttttg atgcgtatct tcacttttcg gaataaataa
     2221 attaagtaga tttcgctgtt ccttagaatg ttgacaagga aacaaaatta ctatacacta
     2281 tttttgtttc caagttaaca ttattgcata aaggagtttt tacatgtaca gtttcacaaa
     2341 taaacattct aaagtttttg gatacgtctc tgtcttattc actggattag gatttactat
     2401 cgtcagtcct gtacttccgt ttttaacgct gccttattct cactccatac atcagcaagc
     2461 tttctatatc actttgctca tgtctgttta tgctctagct gccttcttat ctgcccccat
     2521 tctcggtagc ttaagtgatc attttgggcg tcatccgatc cttattatca gtttattagg
     2581 atcaagtatc ggatatttgc tttttggttt aggaaattcg atatggatgt tgttccttgg
     2641 tcgtatcatt gaaggattga cggcaggaga aatctccacc ttatatgctt actttgccga
     2701 catcacggaa cctaatgaaa gaaccaaagt ttttggatgg atgggtgctt tagtcggtat
     2761 aggaacaacg ctgggtccta ttataggggg tctattagct gagttgggga attctgttcc
     2821 tatatttatt ggagcactct ttactttctt aaatgccgtt tatggctaca catttatgct
     2881 agaaagcctt ccaataaaaa aacgttcagt agatttaagt ttctcccatg taagaccttt
     2941 tcaccaatta caacaattat tcaagatttc atccgttatt cctttattga cagcaggttt
     3001 tgctgtttgg ttggcagctg gctctcttca atctatcttt tctcaatttt cgattgacgc
     3061 ctttcagtgg aaggctgggt tggttggttt aagcttctct ttgattggaa tcttggatac
     3121 tgtctcgcaa ttgttcatta tgcctcgatt gctcaaaaaa ttttcagaac agcagatcac
     3181 aagaataggg atgttcagtg aaatcctcgc ctatctattc atcacattat ctggaattct
     3241 tctactgccg attttgtttt tatttgggat tatctgttat ggtttcggtg attctatttt
     3301 tactcctgta ttcaatggcc aactatccaa ttctgtctca gataaccagc aaggtcttgt
     3361 aatgggcggt acccaaagta tacaatctct atcgcgcgtc atcggcccgc taatcgcagg
     3421 acagctttat gctgcagcac catttcttcc gacggtattc ggatttttgc ttatgatttt
     3481 tgcctattat cactacaata aaaaatagta gcttcatttg ctacttcgta taaattctct
     3541 gtatcgaccg ataaaacgaa atactgtgtc aaattcttct tttgacatat tttcaaatac
     3601 agtagcatct cgctgtaccc atttttgatg gagttgctcg tgtctattcc ttattttttg
     3661 tccgattggc gtcaattgaa aatagatttc ttttttattc tcttcgcttt gataagtgtt
     3721 tatgatccct ctagctatca gtttttttgt tagcttactg atggctcctc tagtcatgta
     3781 actagcattc gctaattttg tcacgttagg atcagggatt ttcccgatat gttcaatcac
     3841 ttctagttca tttagagtaa atcctgcaaa ttctttttcc atttctggtt tattacgatg
     3901 aataatctca ttagctaaat ccagatattc ttctattagc agttgtcttt tatccatttc
     3961 tacaccttct ttttctttca ttatattgtt tttttgtttc ctattcaaca aagataaaaa
     4021 tattttgtag gaaaacccaa tctgaaacca agaaa
//
LOCUS       DAYYHX010000206         4034 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000206 DAYYHX010000000
VERSION     DAYYHX010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.208"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..775
                     /locus_tag="ACHA6Y_002512"
     CDS             89..775
                     /locus_tag="ACHA6Y_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8988574.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            777..1721
                     /locus_tag="ACHA6Y_002513"
     CDS             777..1721
                     /locus_tag="ACHA6Y_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8988575.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1920..2564
                     /gene="pcp"
                     /locus_tag="ACHA6Y_002514"
     CDS             1920..2564
                     /gene="pcp"
                     /locus_tag="ACHA6Y_002514"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8988576.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2643..3920
                     /locus_tag="ACHA6Y_002515"
     CDS             2643..3920
                     /locus_tag="ACHA6Y_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988577.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         1291 a          694 c          889 g         1160 t
ORIGIN      
        1 agtgtctatt tcgtgagaaa acaactatat tttttatgtg ttttgcttta taattaaaaa
       61 aatattgtca ttttatggag gaaaatatat ggaaaattat cttagccttt taggcgtctt
      121 gattgttatt ctcgggtttg catttaaatt agactcgata ttgatcgtaa tggttgcttt
      181 agttgttaca gctttgactg gcggtcttgg cattcaagga atgttagaac ttttaggtac
      241 aagttttgta aacaatcgcg ggatggcgat ttttatcatc attatgttgg caacaggaac
      301 gcttgaaaga aatggtctga aagaatcagc ggcttccttg atcaagcgat tcaagaaagt
      361 atctgctggt atgattgtag atatttatgg gatctttcga atgatctttg cggcttttaa
      421 cgtgagtttt ggtggagttg ctggatttgt tcgtccaatc attttaccga tggcattggg
      481 gaccatcgaa tcaaaagggt tagaagtcca tcctaagcat gaagaagaac taaaaggtat
      541 ggcttcagct atggaaaatg tctgctggtt ctttggccaa gtactattca tcggaggagc
      601 aggggcgttg ctagtccaat caacactgaa agatctagga tatgacgtaa cattgggaca
      661 gcttgcttta gtggaagttc cagtggctat cacggcttta gttgtggcaa gtgtctactt
      721 ctatcttaaa gaaaagaaat tagcgaagaa atattatcca ggaaaggggc aataagatgg
      781 acttttttac aaatcctgat attttattag gcgataaatt actggagatc ctttatatcg
      841 tgatgggact cgtattgatt tacactggag taagaaattt aacagataaa accaatcctc
      901 atcgttatgg gtcagccttt ttctggacgg tcttaggggt agttattgca ggaggaagat
      961 ggcttccggc aatcgtgaac ggtggcttga tctttgcgat gaccatacca gcaattgcta
     1021 agaaagtcag caaaggagaa agccggcttc catcaaaaga atatatggaa aaaatgtcag
     1081 acaaactagg aatgaaaatt tttattccag ctttgagtat cggtgtcttt gcgatcttgt
     1141 ttgctctatt taccaattta ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca
     1201 tgctaatctt gatgttcttt tctcgagata atcgaccaag tacattttta gatgatgcag
     1261 cagatatgct gggcacagta gggccgttaa gcatgttgcc aatgctttta gcttctctag
     1321 gtgcggtatt tacaagcgct ggagttggga cagtgatttc caatgcagta ggtacgatcg
     1381 ttccagaagg aaatgtgaac atcggcatca tcatttacgc tcttggcatg gtgatattta
     1441 cggtaatcat ggggaatgca tttgcggcca tcaccgtgat gacggttggg atcggtgcac
     1501 cattcgtatt tagtcatgga gcgaatccag cgttaatagg aatggttgca ttgacttgcg
     1561 gtttttgtgg gaccctgctg acaccgatgg ctgctaactt caatatcgtg cctgtagcga
     1621 tgctggagat gaaagacaaa tacggagtga tcaaaaacca gttgtttatt gcgttattta
     1681 tgctagtttt ccaaattgct tatatgattc tatttaaata atagaagaga ggggttgtga
     1741 cagaataatt gtcgcaacct cttctaacat tgtagtggaa cactattttt ttataagaat
     1801 atattaagtt tgatctgtta tgttatttaa actattgttt cctttattta cgctgttcaa
     1861 ggaaaatggt atgataaaag ataaccatat gttaaatcaa tcggacggag gaacaacaga
     1921 tgaaagtact agtaacaggt tttgatccat ttggtgggga caaagtgaat ccagcctatg
     1981 aagcagtaaa aaaaatgcca gatgaaatca gcggtgctga aatcatcaaa gtggaagtac
     2041 cgaccgtatt cgaaaaaagc agtcaagtag tcaaagaagc catccaacag caccaaccag
     2101 atgtcgtgat ctgtgtcgga caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg
     2161 caatcaatct agcagaagca agaatccctg ataatgaagg aaatcagcca ttcgatacgg
     2221 ctttagaaga aaacggtcca gccgcttact ttacctcatt gccaatcaaa gcaatgacaa
     2281 aaaatgtaca agatcatggt ctaccagcgt acatctctta tacagcagga acatttgtct
     2341 gcaatgatat catgtatcgt ttgcttcatt tgatcgaaac acaattcccg gcaattcgag
     2401 gtgggttcat ccacgtgcca ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc
     2461 cttctatgtc tttagaagac attgcttctt ctttgactta tgcagttgaa gcagcagtag
     2521 aaaataaaga agatatttcc ggaaatgccg gaacaactca ctaatttagc aagaaacact
     2581 ggctggttgc taacgttatg ttagtagcca gctttttgta attagaaagg aaagaaacag
     2641 ccgtggaaga atggcaatcc gtctttgaag aatggtttcc aaaagaaatc agcaaaagct
     2701 atcccatcaa aatttctaaa caatacacaa gcagtcaacg ttgggagatc tatgcaaaat
     2761 taacgaaaaa acagcgggaa ctagtcgata agcaccgccg atatctaatc agctctcgtt
     2821 ttatggaaga acattattta gctgcaacag attgggtgtt ttctgacttc aagatcaatc
     2881 cgttttttcg aacaaaacga agccagcaaa aattgtattg tgagtgtggt agagaattaa
     2941 aagtgcaata catcgttaaa tcgccaaaaa ccggaaagat cctaaaacta ggcatcaacc
     3001 actttgccga tcacctgcat gtctctccga cagtggcagc ttcgatccat cagggaatga
     3061 caaaagttga tctggcctta gacgaattat tatggcttaa gcagaagaat atcgattttc
     3121 ctgaaggact atggcaaaaa tactgttttg tcttgtacca aaatcgtcgg atgaaacagc
     3181 catatttacc ggatataaaa ttagctcagc ggctcgcaga atttcgccaa gtagaaatgc
     3241 cgatttacat agcggattat caagcattgg aaaatgaaat caagaaaata agtgaacaca
     3301 tcaatgggca gtcaaaaaag cgacagataa aaaaagaact atttgacgac tttgcagaag
     3361 aattagtaaa ggatgtagaa gaattcttga tcaactatcg cgcattttta cgtaaagatt
     3421 ggcaatccat cgtatatgaa gaagtgccag tacatcctaa tgcttatttt gaaacattta
     3481 tttccgtgtt gagaaagaca aaacgtcaac gaacaccaga agtcactgct cagatggaat
     3541 attttgcgaa aaaccaacga ttcatccaac caaaaatcta tctatttatt tggaagcaat
     3601 attgtcgcta tggttttaca gaagggttct tcgacagcat tcctcggata gtcagaaatg
     3661 gttttttaaa agtattgcgt aaagaacgag aagctataca atctgctgat aagaaagacc
     3721 gtacagtctc aaaagaaaaa tggcaactag tagtgaaaga tatccaatct ggaaatgtgc
     3781 aagagacaat cgacaaatgg aaaggaaaac attatcgttt tactgaagct cagaaacaag
     3841 cgttggaata ttatcagaag ttagaagaat cactgcgttt caatgatgag gcaagaaagt
     3901 atctgaagga attactataa aaaacatact aagattagag gggaacatca ttgacgaatg
     3961 atgttctcct ctattttcta tttttagatt atagtgaaga gtgaaagaaa gttcgaacaa
     4021 agccttgagg acta
//
LOCUS       DAYYHX010000207         4021 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000207 DAYYHX010000000
VERSION     DAYYHX010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.209"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            469..1641
                     /locus_tag="ACHA6Y_002516"
     CDS             469..1641
                     /locus_tag="ACHA6Y_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFM8988578.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            1753..>4021
                     /gene="esp"
                     /locus_tag="ACHA6Y_002517"
     CDS             1753..>4021
                     /gene="esp"
                     /locus_tag="ACHA6Y_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM8988579.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT         1441 a          673 c          770 g         1137 t
ORIGIN      
        1 aaaaaagttc aaaagatcaa gtgtgcgctt cgctcccact cccctcgacg ttttagtagc
       61 ctttccctca cttcgttcag tccaagccaa ctaaaaagtt ttcgggatac attctccttc
      121 tcttcctaat aatcaattaa aattttaatc tttatatttc tgctaatcac tcaggaaaca
      181 gtacagaaaa acaaacacgt atcatagata taaatgtaat ggcatagtgc gggttttatt
      241 ttcagcctgt atcatagcta aacaaatcga gttgtgtgtc cgtttttggg cgttctgcta
      301 gcttgtttaa agtctcttga atgaatgtat gttctaagtc aaaaaactct gacagcgcct
      361 ttatatagat aaagtccgta taattgtgta aaagtaaaaa ggccatataa cagtcctttt
      421 acggtacaat gtttttaacg acaaaaacat acccaggagg acttttacat gacccaagta
      481 cattttacac tgaaaagcga agagattcaa agcattattg aatattctgt aaaggatgac
      541 gtttctaaaa atattttaac aacggtattt aatcaactaa tggaaaatca acgaacagaa
      601 tatattcaag caaaagaata tgaacgaaca gaaaaccgac aaagtcaacg aaatggctat
      661 tatgagcgca gctttacgac acgtgtaggc acgctagaat taaaagtacc cagaacacgt
      721 gatggccatt tttcacccac agtgtttgaa cgttatcaac gaaacgaaaa agccctcatg
      781 gcttcaatgt tggaaatgta tgtatcaggc gtttcaactc gtaaagtatc aaaaattgtg
      841 gaagaacttt gtggtaaatc cgtctctaag tccttcgttt ctagcttaac agaacagcta
      901 gaacctatgg ttaacgagtg gcagaatcgt ttattatcag aaaaaaatta tccttactta
      961 atgaccgatg tactctatat aaaagtacga gaagaaaatc gagtactctc aaaaagctgt
     1021 catatagcga ttggaataac caaagatggc gaccgtgaaa ttatcggctt catgattcaa
     1081 agtggcgaaa gcgaagagac ctggacaaca ttttttgaat acctaaaaga acgcggttta
     1141 caaggtacgg aactcgttat ttctgatgcg cacaaaggat tagtctctgc cattagaaaa
     1201 tccttcacca acgtaagttg gcaaagatgc caagttcact tcctaagaaa tatctttacc
     1261 accattccta aaaaaaattc aaaatctttc agagaagctg ttaaaggaat ttttaagttc
     1321 acagatatta acttagcgcg tgaggctaaa aatcgattga ttcatgatta tatcgatcaa
     1381 ccaaaatatt caaaagcttg cgcatcattg gatgatggat tcgaagacgc ctttcaatat
     1441 accgtacaag gaaattccca caatcgacta aagagtacca atctaattga acgactgaat
     1501 caagaagtac gcagaagaga aaagattatt cgcatcttcc ccaatcaaac atcagccaat
     1561 cgcttaattg gagccgttct tatggaccta catgatgaat ggatttattc ttcaagaaaa
     1621 tacatcaatt ttgataagta gaaatggtaa aaacattgta tagcatttta cacaggagtc
     1681 tggacttgac tgtatcagat ttatctttta ttcttactaa gaaaataact attaagggag
     1741 ttgatttgaa taatggttag caagaataat aagagagtat ttctagaaaa aacaaagaaa
     1801 cgagtattga agtacagtat caaaaaatta agtgttgggg ttgcatctgt tttggttggt
     1861 gttggtcttg tcttgggaac aactgaacta gttcaagcac aagatgaaat ttctccaagc
     1921 accccacttg aaacagcgat aagctccgta caagtaggtg acaaagtagc ttcggggaat
     1981 acttttcagg agaatcctgg ttatacgaaa aattataact tttcggattt acaatttagt
     2041 cctcaagaac taactggaga cacactaaaa ggcaatacaa ttggatttga agtttacgga
     2101 aaacataata ttgcagcatc aactaaaaac tgggaaatcc gtcttcaatt agatgaacga
     2161 ttagctaaat atgtagaaaa aattcaggtc gatccaaaga aaggaattgg atcttctaga
     2221 cggacctttg taagaattaa tgattcgctg ggtagaccta caaatatttg gaaggtcaat
     2281 tatattcgtg caagtgatgg attatttgct ggggctgaaa caactgatac acaaactgct
     2341 cctaatgggg taattacgtt tgaaaagagc ttagatgaaa tttttaaaga gataggcata
     2401 gacaatctca agaccgatcg tttaatgtat cgaatctact tggtaagtca tcaagacgat
     2461 gataaaattg taccaggtat tgatagtacg ggctactttt taacagattc agatgatttc
     2521 tataatagct tagatgtatc tgaaaataat cctgaccagt ttaaacatgg atcagtaagt
     2581 gcaaaatatg aggaacctaa tactcaaaca aaagatggtt caggatccac aggtgctaat
     2641 ggagctataa tcttagatca taagttaacg aaaaattata atttctcata ttcagcttcc
     2701 gcaaagggca cgccttggta tgctaattac aaaattgatg aacgattagt tccatatgta
     2761 gcgggtatac agatgcacat ggttcaggct gacaaagtaa catatgatgt ttcttttgaa
     2821 tcagggaaaa aagtggctga tttagcaatt gaacggcgca aagatcatga aaattatggt
     2881 gtaggttcaa ttactgataa tgatcttacc aaacttattg attttgctaa tgcaagtcca
     2941 cgtccagtcg ttattcgata tgttttacaa ttgactaagc cattagatga aatcttagaa
     3001 gatatgaaag caacagcaca agttgaagaa aataaaccat ttggtgaaga tttcatcttt
     3061 gattcttggt tgtcggatac gaataaaaaa ttaatccaga acacttatgg aacaggttat
     3121 tattatttgc aagatattga tggtgatgga aaccctgacg ataaagaaga gagcggagac
     3181 acgaatccat atatcgggaa acctgaatta gaagaagtat atgatgttga cacaacagtt
     3241 aaggggaaag tattcatcca cgagttagcg ggaacaggtc acaaagccca acttgttgat
     3301 aaagaaggta ctgtattagc agaaaaaact atcgctccaa atgaaaaaga tggggctcca
     3361 atttcagata ctgtagaatt tgaatttacg ggtgtagatt caagtaaatt aatcgcgaaa
     3421 gatgaattaa aaatccaaat cgtttctcca ggttttgata aaccagaaga aggttcaacc
     3481 gttattaagg aatcaccaaa agcggttgat aaacaaaccg tggtagttgg atttaaacca
     3541 gatgctaaag aatcaattcg gaataataaa aacttacctg aagatgcaga gtattcatgg
     3601 aaaacagagc ctgatacttc taacgttact gatagtacga aaggtattgt aactgttaag
     3661 atcggaaatc gaactttcga cgtggatgta gagtttgctg taaaagcttc tcaagctatg
     3721 gaaaatgatg caacatacgt acctataaca acaactccag aaacgacagt tcaaagtgga
     3781 aaacctacat ttgataaacc agatgttcct ttagctaaag atgccttttc aattttagat
     3841 gtctataata aggacttcgg taatgcaagt gttgatgcaa atactggtat tgttacattc
     3901 actccagcta aaggtgtagg agaatcggag ccgattactg gaacaattcc tattaaaatt
     3961 gtttaccaag atggttctgt aggcacgacc gatttagcag taactgtaag taaagatatt
     4021 t
//
LOCUS       DAYYHX010000208         4001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000208 DAYYHX010000000
VERSION     DAYYHX010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.210"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2697)
                     /gene="brxC"
                     /locus_tag="ACHA6Y_002518"
     CDS             complement(<1..2697)
                     /gene="brxC"
                     /locus_tag="ACHA6Y_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFM8988580.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNI"
     gene            complement(2722..3297)
                     /locus_tag="ACHA6Y_002519"
     CDS             complement(2722..3297)
                     /locus_tag="ACHA6Y_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFM8988581.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(3297..3896)
                     /locus_tag="ACHA6Y_002520"
     CDS             complement(3297..3896)
                     /locus_tag="ACHA6Y_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFM8988582.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         1197 a          771 c          542 g         1491 t
ORIGIN      
        1 aatattacga ctttgaaaca gttctctacc gatttcgtca accaattttt ttgcttttgg
       61 cgaaatcacc gcgcttggtt caatttttat cttttcactt tctcgtcgtt tttgaataat
      121 cattacaata ctatttggat catccatccg tgaaatttgt acattgttat aaagtaaatc
      181 aattttacca gacatgaaaa gtttagcgat gcaccattcc acatcaagtt ctgtgaatcc
      241 atacggttgt tttgtttcaa aacgatcacg aattgttttc atcgtaatcg ttaggtgatt
      301 tctcttttga tcattgataa aattgaaaat ttcctgaact gcttgctcat ttgatggatc
      361 ttcaataatt tcatttttgc tttctttcac taaatataag atatcttctt tttctttagg
      421 cgcatcaatt tggtttaatt tatggtatac ttcttcagcc attttctgaa gtgctgattg
      481 gaattttgct ttaaagtcgg agcctgtcag ttgaatttct gcaccgttat agaaaaattt
      541 tgcagatttc aacagttcat ttaattgatc gttgatactt gcacttagtt cctgtaattc
      601 accagatttt tccgaacgaa tcgcgtcaaa gttctttact acactcgaat aagtcgttgt
      661 taaataaaga ttcattctag catacattgc tatatcatct ttaaattctt tatctgatgg
      721 caattctacg aataataagt tacgggaact cttaagcgca agttctgcct cttgacctgc
      781 taaatcagag tatggtgaaa taaaattaac tgtcatttca gcatttccag cttgcttgta
      841 tggacgttga tctaacactt gtgcataagt aaacgtatac cggtttttaa atttatttcc
      901 acctgacatg cctaattttt cataggcaaa actatttcgg tctagaacgc cgtcaaaaat
      961 tcgtttagat agttctacga tcaaatcact atctgtaata ttttgatttt taatctcacg
     1021 agtaatttcc tgttcttcat cagtttggaa aatataacta tctaagttct tccgcacaaa
     1081 gttttcctta attaaaaggt ccaatgcttc ttctactcgt tttttagttt ccaggcggtc
     1141 ttcgttgata tttcttatca tcaagctcgt aatattttca acatttggtt tgatggcttg
     1201 atcaacatat ttaatcatga ataaaattcg taagacattt acgttaaagt tatctatatc
     1261 agttggtctt tgaatatgag aattatttgt agcttgtgta atcactcggc taattgctgt
     1321 atcaatccaa cgttctaacg agtaataaaa ctgatctaaa gaaaccaaac tggttgtatc
     1381 attttcctta tttcgttcag cggcttcttt aaacattgct agtaatgaac gttcaccaga
     1441 cgataagttt gatccttgat agctgtgatt acgaatctcg gttagaattt gccccaataa
     1501 attgaattga tagggaataa atggataaac ttctgcgtaa ttttcagcat cttggaagag
     1561 ctttaattca ggactatcaa caaagagtaa tttatttttc aattctgttt gaacagtttc
     1621 aaattgtaag gctaaagaat cattggcaaa ctcttttttc ttcaaaatac gttctttaat
     1681 tacttcatcg gcatttgctg aagataaact cacagttgtg gcaaaacgat ccttgatctt
     1741 agaaaaatca ttatcagaat tgttatcaaa gtttgttacg tgatcaatcg cttcctgact
     1801 tgtcactaat acccaagcct taccttcaca aattcgaccc aaatcttcca caacggtttg
     1861 taactgcaaa gtcaactcac gattaccgcc gatatattgt ccgacctcat cgactagaaa
     1921 ggcaatatga tggtctgctt ctttggaatc aagatagctt ttaactagat ttgcaaagtc
     1981 atcaatgctt attaaataat ttcctgatgc tttttctaaa aaattatcaa tagcggcttc
     2041 actcataaaa tcgattttaa gtgccgcttc tttgatatca tcttggatcc aatcaaactc
     2101 atggcgctct tcttgccatt ttgccccatt aatctcttca aaagcagctt tgaatgcatc
     2161 aaacttttct tctttaatta accgacgttc aaaatctgct agatgtggaa tcgtgcttaa
     2221 tccttgcatt tcgttaaata cctttaaaaa aacattcacg attccgtttt tgtctttttt
     2281 accacctgca tcactttttg aatcaatatt aaacagaata acatctgtat ttatttgtga
     2341 gcaccgttcc atcgttgcca aagtcagtgg atctttgatt ttcccatctt ctttgaagta
     2401 atcaatggct ttcttgtcat ttatttctcg atttgcgagc aagtaagaaa taatttttaa
     2461 gaaatgagat ttacctgaac caaagaaccc ctgaatccaa acacccattt tatctgttgg
     2521 tgtattgata ctccgttggt aggcatcaaa aattgtttta aagtgttttt gtagttcttt
     2581 cgtaaccacg tattcttcta attcttgttg aacattttca tcctttgctt gaccgacctt
     2641 aataactcct tgaatatttc ggtcaattgg ttttgcaaaa atatctttaa ttagcattta
     2701 atctcctacc ttccatccca actaaattct aaatgctcga taataattat catctttatt
     2761 ggtattaaga attgtcaaac ttaattcatc atattcccct ggatacatca tcacaactgg
     2821 tacgcgatca aatacctgat gcattttatt taaaatagag tgcgctctaa ttaatggata
     2881 taattcgcca attcctgtca ggaacacaac tgatttttct ggtgtatggg catccacata
     2941 ttcaaaaaat acattttctt cctcaggtaa atccattttc aataaacgac caatgctttc
     3001 cactaatttt tgtaaaccac gtcgttcttc catcttaatc gctttttcag tgaaaccgcg
     3061 tttttctaga tactctaaca taatcacata gagattataa actttcactt cataattatc
     3121 accaacagat gttccagata tatcattaat ataatctctt acaattaact cgtctttcgg
     3181 atcgtagtca aagatataat agcctacttc attacctaaa ccacgattca ataaaaaatc
     3241 ttctttttga attacttcat tcaacttttc caaatgctct tgaatcgata atgacattat
     3301 aaaattcctc ccagtgctag tacaatatct ttacgattct tgtcaactaa ccattgaatc
     3361 aatcgtttat ctaataatat cggagtcatt cgtccatatt ctttttcccc tctgactaat
     3421 cctgtttcaa ttagattcgt ttgataagcg gcaataagcc gtttaatcgt ataatctttc
     3481 cattttgaaa tatcttctga acttctccgc ttctctgtaa aaaaagaacg gtaatcaact
     3541 ggtgttaatt gcaaatttcc tttacgtaga ttttcagcat atacctcagt cataaattca
     3601 ttaaaaagat catcaatcat aaggatggaa atcaaattca ctaacttttg attatctata
     3661 tttaactgat tgaaaagttg caatccagct tcatccagtt gactaactct ccttttcacg
     3721 ttctgataga tttctttttg acggttactg ctcactgcaa aaaaaatatt ttcatgttca
     3781 tttttctctt taatttctat atctgtatat ccttctagtc gtaattgaat atataacttg
     3841 aactctgaaa accaaaagcg tttagaaact agaccagcag aatatttttt attcaataga
     3901 ttgcctccta atcatctata attcaataag taaacaagtg ttttttgctc atcgttagca
     3961 ttcatttttt agtatctact atacaataca tatttattat a
//
LOCUS       DAYYHX010000209         3974 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000209 DAYYHX010000000
VERSION     DAYYHX010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.211"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..1588)
                     /locus_tag="ACHA6Y_002521"
     CDS             complement(83..1588)
                     /locus_tag="ACHA6Y_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8988583.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1585..2271)
                     /locus_tag="ACHA6Y_002522"
     CDS             complement(1585..2271)
                     /locus_tag="ACHA6Y_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8988584.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2464..3885)
                     /gene="gndA"
                     /locus_tag="ACHA6Y_002523"
     CDS             complement(2464..3885)
                     /gene="gndA"
                     /locus_tag="ACHA6Y_002523"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM8988585.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1093 a          848 c          684 g         1349 t
ORIGIN      
        1 gacatccctg aatttctcac ttgcttgtat tttgtacctt attctttgtt ggatatataa
       61 aaaaacaagt aaagcacatg ctttacttgt cttctgcctt tgcgatagga ataaagattc
      121 taaaaattgt cccttgccct actacacttt ctgctaagat acgtccttta tagttttcaa
      181 ccagttgttt agcgatagac aaccccagac catttccacc cttcgttcta gctcttgctt
      241 tatccactcg ataaaaacga ttgaagatct tattaaggtc ttctttgggt attccttcgc
      301 caaagtcctg aatcgcgatc tcaaattcat tcagagtgga agaaatagag atatggactt
      361 cttttcgtgt agttgaatac tttactgcgt tatccaatat gataatgatt aattgttcaa
      421 agtgattacg ataaatttgc agggtcactt cttttgtcaa atcatcatct aaagtgatca
      481 catagtctgg atataatatc ttgaaattat tataaacttg ataggtgact tcttttcctt
      541 tagttgtttc attcgcataa tgaacatcta cttgttctgc tcttgataag tctagcatct
      601 cttgaactaa gcttttcata cggctgattt cttgcatgct ggcgcttaat gattcttcta
      661 atatctcagg atcatctttc ccccaacgat ttagcagact aagatgccct tcaataattg
      721 caacaggtgt ccttaactca tgagatacgt cttccacgaa ttgctcttgt tgttcaatat
      781 acaaacgcat acggtcaagc atctcattga atatttcagc taaatctgcc aattcatcat
      841 ttgtgtcgat ttcaggcatg tggatatctg attgcggatc tttacgtata gtatccattg
      901 tatctctgag taccttgagt ggttttaaga aataagaaga taaaaagaaa cctaaaatac
      961 tacttaagat caaagaaaca acttctaaaa caataagagt caataatagc ttatttcgaa
     1021 tatcatagaa agaagataat tcatagaagc tttgcgcata gccgatcttt tctcttgtac
     1081 ctttagaata tataggctgg atagataaaa agccagtttt tccttctagt gtaacgatcg
     1141 ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca
     1201 agttctcgtc caaatcataa acataaagat ccattgatgg ctgccctagc tcagctataa
     1261 aggaatcgat cttcattaag tttccttcga ccagtgaagt atgatctacg acctcatctc
     1321 cgtcatttgt cgctttcgtc agattacgat acgtatttac taatgtcagt tcttcatccg
     1381 agttagccaa acgagaagct acttctgaaa tcgtacgctc tacattgttt ctttcttttg
     1441 ccacaatcag attgatagat gatttatagg taatgacagc gaaaattgta aacacaacga
     1501 atatgaagaa agaactcgca aaggcccact tgattgtcaa ggagggccct ttcagttctc
     1561 ttttttccga ttttttcggc tttatcacga gcgcatcaca taacctgttc cgcgtacagt
     1621 ttggatatag ctttcctccc cgggaacgtc aattttgttg cgaagataac gaatatatac
     1681 gtccactacg tttgtttcaa cttctgtttc gtatccccac actttattta gtaaaacgtc
     1741 acgtgctaat accacgttca cgttttccat taaagtaagc agcaactcgt attctctttt
     1801 tgttagttca attacttcat ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa
     1861 atcgcgataa gtaatagtag tttgtttagc cacattttta tcgccttcaa tatcgattcg
     1921 acgcaacaat gcacgaagac gtgctaataa ttcttcgata gcaaatggtt tgacgatgta
     1981 gtcatctgct ccgtggtcaa gtccagatac acgatcgatc acagaatctc ttgcagtcat
     2041 cataatgatt ggtgtatttt tgacttggcg tacacgtctg cagacttcta aaccgttcaa
     2101 ttctgggagc atcagatcca acaatatggc atcccactca ttatttaatg ccgcatctaa
     2161 accagttcgg ccgttgtagt ggacttctgc gttataccct tcatgcttca actctagctc
     2221 gacaaatcgt gccaagttct tttcatcttc aatgattaga atattgctca tttattattt
     2281 tatcctttct attctactgg ctttttaaaa actctatcat tataccgata atatgcttaa
     2341 aaatctatag tcattttatc atggatagca attttaaaaa agagctgaag tctactcata
     2401 acaatcgact tcagctcttt cgtcctcttt ttattttctc atttcctaac agccgaaaga
     2461 ctattattct tcatggtacc atgagtaatg gaagattcct tctttgtcga cacgctcata
     2521 ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata tttgcaggca gacgttctgc
     2581 tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc ggtacaggta cgccagcttg
     2641 gacagccaag gcaacgacgt ctctgactga ttgctgatat tttttcgtga tttcaacgaa
     2701 gtaatcatct agcaataaat tttcgatctc aggatttttg tcataggcat ccgtaatttt
     2761 ttgcaagaat cgagctcgga tgatacatcc agcgcgccag atttttgcga tttcaccaaa
     2821 tggcaagtcc caatcaaatt cttttgaagc gacacgtaat tgagcaaatc cttgggcata
     2881 actcatgatt ttactgaaat acaacgcttc tcggattttt tcgaccaatt cttttttgtc
     2941 tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc
     3001 tttataagca gaaatatagc gggcaaatac agattcagtg atcaatggaa gtggcacacc
     3061 taagtcaagc gcgctttggc ttgtccattt acctgttcct ttattcccag ctgcatcaag
     3121 gatcacatct actactggtt tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc
     3181 agtgatttct atcaaatagc tgtctaattc gccttgattc cattctttaa agatatcagc
     3241 catttcttct actgaaaggt ttaaaatatt cttcattaag tcataagatt cagcaatcag
     3301 ttgcatatct ccatactcaa taccgttgtg taccattttc acataatgtc cggctccatt
     3361 tgggccaata tatgtgacac atggtgctcc gtcttctgct tttgcagaaa tttgctctaa
     3421 gattggtgca actagcgcat aggcttcttt ttgtccacca ggcatgattg aaggtccttt
     3481 taaagctcct tcttcaccac cggaaacgcc tgtaccgata aagttgatgc cagagttggc
     3541 taactcttca ttacgacgca tcgtatcttt aaagaatgta tttcctccgt caatcaaaat
     3601 atcgccttta tcaaggtgag gaagtaactc ttggattgtt gcatctgttg ccggtcctgc
     3661 tttgaccatt agcaagatcc gacgtggttt ctcgatagat tctacaaatt cttcgattgt
     3721 atatgttgct ttgaaatttt tatctgggtg ttcttctact acagcggttg ttttagcccc
     3781 tgtacgattg aataaagcca ctgaatagcc gcggctttct atgttcaagg ctagattttt
     3841 acccataacg gccataccga caacgccgat ttgttgtttt gacatatgaa ctcctcctac
     3901 atacgggatt tctccctaat ctcttacatt atagcttaac acggctacat tgcaaactaa
     3961 agaacaacat attt
//
LOCUS       DAYYHX010000210         3947 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000210 DAYYHX010000000
VERSION     DAYYHX010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.212"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..912
                     /locus_tag="ACHA6Y_002524"
     CDS             82..912
                     /locus_tag="ACHA6Y_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFM8988586.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(1156..1405)
                     /locus_tag="ACHA6Y_002525"
                     /pseudo
     CDS             complement(1156..1405)
                     /locus_tag="ACHA6Y_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1596..1790)
                     /locus_tag="ACHA6Y_002526"
     CDS             complement(1596..1790)
                     /locus_tag="ACHA6Y_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988587.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(2295..2480)
                     /locus_tag="ACHA6Y_002527"
     CDS             complement(2295..2480)
                     /locus_tag="ACHA6Y_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988588.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(3330..3671)
                     /locus_tag="ACHA6Y_002528"
     CDS             complement(3330..3671)
                     /locus_tag="ACHA6Y_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8988589.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(3671..3901)
                     /locus_tag="ACHA6Y_002529"
     CDS             complement(3671..3901)
                     /locus_tag="ACHA6Y_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8988590.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT         1279 a          732 c          649 g         1287 t
ORIGIN      
        1 attcgcgagc gctttctttg aattttttaa tctttcgtag cagaatgaga atgtattaaa
       61 aatagattag gaggaatttt gatgtttaga caccagaaag aattgcagtt tgaagcaaaa
      121 ccagatcgtc cgaatccatt aatcgcgaaa tatctgcagg agctgatcgg cggccaatat
      181 ggcgagatga gtgtagctat gcagtacctg ttccaaggct ggagctgccg aggagaagaa
      241 aaatataaag atatgctgat ggacattgca acagaagaaa tcggccacgt ggaaatgttg
      301 gcgacaatgg ttgctcgttt attagagggt gcgcctgttg aagatcaaga agcagcgatg
      361 caaaaagatc caacaatcgg tgccattatc tctggaatga atccacagca cgcgatcgta
      421 gctggcttag gacctcgtcc tgctgacagc gtgggcaatc catggagcgg tgcctatatc
      481 attgcgagtg gcaacttgtt agcagacttt agagctaatt taaatgccga atcacaagga
      541 agacttcaag tagcacgtat ttacgaaatg atcgatgatc gtggcgtaaa agatatgctg
      601 tctgttctcc ttgcaagaga tagctatcat caaaatctat gggaatgtgc agtgaaagaa
      661 ttagaagaaa aagaaggttc aatcttagtc ccaagtactt tcccgcgtga caaagaacga
      721 aaagacattg cttatgactt ctataacttc tcagagggag accaaagtag tgaaggttct
      781 tgggctagtg gcaaagcact ggatggcgaa ggcgaatatc aatggttgaa agaacctaag
      841 atcgaaggcg ctgcaccaaa attagaacca gtaacaccaa aaatgtatgg gacacctcca
      901 ttcaagaaat aaatatctac ccgtagaaac acccgttttt tgttccacac gctttgtgag
      961 caaaactgac taaagtcatt aatcataacc acccttcaaa agggtggttt tctctagagc
     1021 tataagctca aatcaccggc cagcgcctaa agacgctggc tttcatattt tatgttcaag
     1081 ccatattgcc tttgccagtt ttgcccacct cttaaaaggt ttatgtacta ctccttagcc
     1141 cttaaaagga tcgtcttact ctcgtacact cagcttatct aacgcactat catgtttttc
     1201 ttgttcttga atgtactttt tgatggtcgc ttcattttgt cctacttttg tcacatagta
     1261 tccttccacc caaaagtgtc gatacccaaa tttatatttt agatttgcac aatctcttga
     1321 tgatctcctg aatattggct cgatattgat tataaattat ttttcttcta tactttggtg
     1381 tgaatacaat gtggtattta cacatctact ttgtacgggc taaactattt ccctttattg
     1441 ctccacccgc atagcaagtg gttttgtatt tcaatcgctc ggcgcgctca attaggtcta
     1501 aagacaataa atctaaaaac ccgctgcttc actgacagaa acaacgggtt ttcagaaaaa
     1561 aatgaaatga aaaagatctc ttacggcaat ctattttatt tttttgattt tttataaatg
     1621 gaaaatgctt caagaagtcc aagaaataaa gccgtataaa aacaagaacc tatgaaatct
     1681 ttatttatta gatattcaac agaaatcccg ataacactag ccaacaatat atttatcaga
     1741 agtgtaaaat aaggatgctt tttatagaat agaactagtg ttgttttcat agactctcct
     1801 ttcttcatat ttccaattaa tcacaaattc acggcattac tagaaaaatt aaaggggcgc
     1861 agaagtggaa aaatactctt cgactttccc tacaaaccag gccatcttgt gtttaataac
     1921 tataccaaat ctaaggtacc tctaaatcta aattccattt gtggcggtga cctccaattt
     1981 ttaactcttc tatctcccat ccagtataca taaccttgat acttcgacca cttatatatc
     2041 gaacacacaa atacctatcc attgcaggag tactacctaa cttagttgct acttgcatct
     2101 aaataacagc tgaaaagata ggacttactc ttagtaaagt atctgaacta ttaattttta
     2161 aactatcatt tttatcctca actacatgca catctgttgc aacttcactt tctgtctcat
     2221 ccagataaat gattaaggaa tcgggaattg caatatcttc agctgacgct ggaatagttg
     2281 agctaaatta gtcatcatat ctttattata aaataaccaa cagcaaaaaa tacaatgaaa
     2341 aacaaacttg gaactaatgt atcattaata aatgttgaaa aattcagcaa ctcattactg
     2401 tatgatttta gcagcccaac caacatatac cctataaaag ctaataagat ggttaggaat
     2461 cctttagaat ttttactcat tcatagacac ttccttaaat aacttttatt atagaaggta
     2521 ttgtaaataa taatattgac aaaaagtgac tcaaaataaa aaaaacctta cattatactg
     2581 gaatgaactg tattgatagt gtacctaaaa gctctactga cataagatcg tgtaatcggt
     2641 aaagtaattt tattaattat acgggatgta atcaattaga gaataaacta atcataaaag
     2701 gtacttttac attcataatt gtcgctttga gattctactt tggttcacta tctgacccaa
     2761 ttgcacattt tcgatcatta caagtattgg tgtcgttaga acaactaaat aaaaactagc
     2821 ggtagatttt tcagaatcta tgctaatttc tgtatggatg ttccagtgat ggcaaatttc
     2881 tcttttcatt gtttttcttg tcgcatgata tgcctttttg tgttattcag ttaaactata
     2941 gaaattcttt cctagacaga tcttgatccc aaattttacg tttttgagca tcgatatagt
     3001 ttaaaatgac attactcata tctaaagagt ttagattttt taggactaaa actgtcagtt
     3061 gttctttttc ttctttctgt taaagtattg tcgcgacttt tcgtatacgt taagatatgt
     3121 cgtgaaaaaa caagttttgt gacacaaaaa aacgaatgac ttgaggaaat cacctcaatc
     3181 attcgtttaa aatgctatat attctttagt ttaagatacg gaaattaatg ccatcgacca
     3241 cactagtaga atgaacgaca tttacttggc ctttttttaa tttcgttatg tttagcatat
     3301 aaaaatatta tagagtttac ttacaatgct taaaacatat actgtataat ctgatcaatt
     3361 ttagccatgt cttgcaatgg taagttttct atttttttga gttttcgttc cttaaaatca
     3421 agagatttta attgtgtaat aattacttgt ccttttatat ctagctcttc aggtaatgag
     3481 taacgtgtcg gtaaattctt tatagtggaa gtaattggac atatgaccga aaacattgtt
     3541 ttacggttaa attcataacg agagacgact agtcctggtc gtcttttttg aatttctttt
     3601 cctacggagg gatcaaaatc aatccataca atatcacctt ttttaggaat ataaattctt
     3661 tcttctgaca ctataaaatt tctctccctt caggagttaa atcttcccat tcgtctttct
     3721 cataatactc ggcctcttcc ccaccactaa atggatcttc aatttttgga actaacgtaa
     3781 ttgttccatc atcagaatac acaacgatat attcttggtt ttctgaaggt ttctttccgt
     3841 tatcagaagg taaagttacc actacagaac ttccttgtaa tctagatttt gtaacaagca
     3901 ttatcatcac tcccttaata aaagtatact atataacact aagtaac
//
LOCUS       DAYYHX010000211         3919 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000211 DAYYHX010000000
VERSION     DAYYHX010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.213"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(209..358)
                     /locus_tag="ACHA6Y_002530"
     CDS             complement(209..358)
                     /locus_tag="ACHA6Y_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988591.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            820..1032
                     /locus_tag="ACHA6Y_002531"
     CDS             820..1032
                     /locus_tag="ACHA6Y_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988592.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1310..3109)
                     /locus_tag="ACHA6Y_002532"
     CDS             complement(1310..3109)
                     /locus_tag="ACHA6Y_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM8988593.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3233..3829)
                     /locus_tag="ACHA6Y_002533"
     CDS             complement(3233..3829)
                     /locus_tag="ACHA6Y_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8988594.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1398 a          698 c          679 g         1144 t
ORIGIN      
        1 tatcatcgaa acagctaaca aaattgtcat aaaaaacgag atggttgata ttagaacttg
       61 tgtttttgtt taggtctaga gataaagaga gacaatttcc aaatatatgt aatgaataca
      121 aaaagacgtt tgtcgcggat taccgttaca aacgtctttt attgattttc cgagatctca
      181 tcagtcgcca tttggaatgg ttcactcctt atctagattc gtttaatgct cggactgctt
      241 gttcgattgt aataccaaac gcaacgttat tttcgttttg tgcatcaatc gccataaaac
      301 ggttagaagt tgtatcaaaa gctacgcggt attgcatatt tgtttttgta aacgtcataa
      361 aattcatctc ctttgtaatg taatttagtt cttgaaagtt tcctttaaac tttcgaactt
      421 ataccaagtt tagcatcgta cgttttttga tgcaattaaa aagcatttat cattttcctt
      481 atttattttt tcttaaatgg tttagctatt ttcttttaat ccgataagta gcccaatcat
      541 caaggttaca aatactattg cttgaatata aatcatatgc tcacctcttt ataaataact
      601 tagcaatata aagaaaaaaa cgcacgaaca acataggaat ataaacaaac gtttttgtca
      661 caacgtctaa gatcgtgtta aaaaatagtt atctaaaaag cagaaagata ctcaaaataa
      721 aaaataaaaa aatggataaa aacgttttca aatacaaggt aatttgttat tataaaataa
      781 agaaaaaaca aaataaaaat aaagaaaaga gggaatgaaa tggtaaaagc agaagtagga
      841 agatcttata tatgcaaacc gatcggagta tctcatgaac taatcggctg tattgaacgt
      901 acatatatca acacggctgt cgtgtatgtt gaaagttatg accaacgaga ctatcaactt
      961 atcaatgaaa gcaagtatcg catgctcgta aaattctgtg atattagcgc acctgctgaa
     1021 ttggtttctt aaacagtatt tttgagaatt cgcaagaact cccttataat ataaataaag
     1081 acagaaggga gcgagaagat atgccaaaaa gaaaaagttc agggaaaatg ggaattattc
     1141 ttgcagtgat ctctgcaatt cctgctattt tagctattgt tcgtgcgatg aaggaatcaa
     1201 gagaaagtta agcacacatt atgagcgtca aacaaaaaac gcatatacct gcggtggcag
     1261 gttatgcgtt ttttgttgta taaagacttt tttcattaac gatttttgat tagccgactc
     1321 ccaaaacaaa atttagcagt ttatctgaat aagtgatttc ttcatctgtt tgtttcacac
     1381 tttcatttag tagtcccatc tcatcggtta tgataccatc cacaccataa aacatcatgc
     1441 gactcatagc atctgaatcg ttgattgtcc atacatatac ttcttttccg tcttggtggg
     1501 cagaattaat gaagttacgg ttgatcgtac tatattccat tgtcagaaaa tcagcaggcg
     1561 tcacaggggg cccaacaata ttgaaaggta agatgtagcc cacataaaag tttggttctg
     1621 cttcttttaa atctgaaacg gtttggtacg tcaaactttg caaaatatga tggttctcta
     1681 agataacttt gcggtattta tggacaaatc gttcgacaag gtcatccgaa tcttttttag
     1741 aggtcttgat ctcaataagt aatttttggt ttagcttatg tgctttttcc aaataagaat
     1801 caaaagaaca aacaggggct tgcactccgt tttcagtaac tgtaagattt gtcaattctt
     1861 tcaatgtaag atcctgtggt gttccattta cccctgttaa gtgacgaaga tttgcatcat
     1921 gcatcactac gaaatgacca tcttttgttt cctgaacatc catctctaca taatcaggat
     1981 gatagtctct gcttgttttt tcaagtgctt ctaaagtatt ttgcgcccca ttttgggtac
     2041 tcacacctct gtgagagaca gtcactggtt ttgtctgaac agctgaagtc agataatcca
     2101 tattgtaaag acagacgcca ataccgaaaa aaactgcaaa aagagtcaaa ccagtgtttt
     2161 tcagcgcaga gaaacgcaag ttgggggctt gtgggataaa ccacttaggg atctcaggca
     2221 agaagccttc gtcatccatg aaatcaacaa tgatataaaa aatccctacg gtagacatca
     2281 cgatattgaa taataaaatc ccttgtaaaa gcgtcatagc aaacacagcg ctgatcaatg
     2341 catagtctgg gaaaaattgt tcaaccatac tttgtgccaa aataacaacg atatatccta
     2401 aagaagaaag gagtaaaatc gtaccgccaa tcacaataaa ttggcccgtg atggccaaaa
     2461 gacgcgattt ggtcaatgac cagctttccc gaatggctgc tcgaaaagga cgatctcgta
     2521 gaatcatttc cggtaaagca aaaatcagtc gaatcccaat atagccaagg aaaatataaa
     2581 cgagtaaaaa ggaactgacg atgatccaac gattagcaaa aataaaatcc ataatgaaag
     2641 cggggatttt tatttttgac agtagatcag aattaaaact taatccgctg atcggtagaa
     2701 tcaataaaaa ataagcaagg aaaaacaaaa aagtaatggg tctcactttc ttcagctgaa
     2761 gtatcgtcaa gtgtaagagt tgcttcaaag agattggttc tttcttttta ataaaaaaaa
     2821 cactcatcaa aagaaaagta aattcgaaat aaactaacag taaaatcgtc atcaaaacta
     2881 cgattaacga gagtagaacc ccaggatgtt gagaaaaaat tgaaggaata ttgtcataag
     2941 acaaatagtc aattgcccct cggtgtaaaa taaaacgggt cgtactgctc aataagggta
     3001 aacaaataaa taaaataaag ccatgcatca gcaagacatc acggaaataa gcttgtgttc
     3061 cttttaaaaa gtcccaagca ttacctaagc tacgtttcaa ataattcatc ctatcacctg
     3121 ctcttcacat tgtagcgcaa atctaatgaa aaaggaaagg gtgcagtgtg ttttgcagac
     3181 cctttcccag atttaacatt gtcaatcaat gactaatttt acaaaataaa tattatccaa
     3241 gaagaccact tagatattga cctccataaa tcaaggccat actatataag aaaatcgaca
     3301 gtggtttagc aatcaaaatg attagtgtat atcgtttcac tgacatgttt gtcaatccag
     3361 ccatcaaaca taacgcatcg tcaggagcaa ctggaagaaa aatagctaga gcaaacagct
     3421 tctcaaaacg gttttgatta tccagccagc caatatattt gttatatgtc ttatcgctaa
     3481 tcatggaaag tatgaatggc tttccataac gacgccctag cagaaaaatg ataatcgaac
     3541 caatgcagat tccaatatag ttatagataa atccagcata agggccaaat atcaggacac
     3601 cagctgcagt actgatccct ccaggaatga ttggaataac tacttgcagg atttggatga
     3661 agacaaaaat aatcggtcct aaaatgatgg aatcacctac taatcctctt aacgcattca
     3721 aatctttgaa aacacctaaa ttaataaaat aaatcgtcag cgcgattgat aggcctaatc
     3781 cgataatgga tatgaaattt atcagttttc gagaggcagc aatattcatt attcatcaac
     3841 tccttagagg ttctttgtta actatacttt actagaaaag caactaactg cgccattttt
     3901 tgatatgaca gaaaacaaa
//
LOCUS       DAYYHX010000212         3878 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000212 DAYYHX010000000
VERSION     DAYYHX010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.214"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..240)
                     /locus_tag="ACHA6Y_002534"
     CDS             complement(<1..240)
                     /locus_tag="ACHA6Y_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8988595.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIE"
     gene            complement(242..1681)
                     /locus_tag="ACHA6Y_002535"
     CDS             complement(242..1681)
                     /locus_tag="ACHA6Y_002535"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM8988596.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            1866..3812
                     /locus_tag="ACHA6Y_002536"
     CDS             1866..3812
                     /locus_tag="ACHA6Y_002536"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM8988597.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1227 a          677 c          647 g         1327 t
ORIGIN      
        1 ctcaatgagt tcagagtaaa agatattact aatattagga aaaataagac caataagctg
       61 aggtgacttt ccttgaagtc cacgtgctaa attatttggt ttataaccaa gagttttcat
      121 agcttcatga actttttgtt ttgttttttc tgataaatat cccttattat taatgacgcg
      181 tgaaacggtt gttgggctaa ctccagctaa ttcagcgaca tcagttaatt ttgctaccat
      241 tttaatattt taattcaaag tattttccag taggttcgcc tgatttgatt tgaataccat
      301 tttggtgatt ttcagggaat acacgactag taaacacttt ttctccttta ttaataaaaa
      361 tctcaatgat tgagttatct acaaaaatgt tagcagttgt ttctttagta tcaattgagc
      421 attcacgaat cgtaccaaag tcagtggcat attgaatgcc tgctttgctg cgatctaaaa
      481 taatttttcc ttctttggta tctacaatca agctaagtcc atttccttta ctatcagaga
      541 aaagaagtaa ctccgatttc tgatttggag gaaaagttag ttcaagctca taagtattat
      601 ttgtttctgc ttttgctgta aattgttcag aactagcacg taatgatgta atagcttcta
      661 ctggatattg gtaaagttta ccatctttta ttgaaagttc ttttacaaga ctcattgctc
      721 cctgataatc atacttatca gttggataat cgatatcagg tagtccaatc caactgacaa
      781 tcagagcacg accatcagga gtattgaatc cttgagtcgc atacgcttca aatccataat
      841 caagattttg aatttctgaa gcgcccacaa gttttacttt ttctatgtca agcgattgac
      901 agatcttaaa agtattcgga tagatattcc cataatttag ctcagattta tccaaccctt
      961 gaggacaata aatgagaaca ggttttttgt ctacaaatac tagatttgga cactcaatca
     1021 tgtattctga accagtgccg ccaaaatcaa ggttgccaac ttcctcccat gtttccacgt
     1081 tattgtcatc cgctttgtaa agtttgataa agcctgattt ttctaaattt tgtgctccaa
     1141 caatcgcata caattgtcct tcataattaa acatttgtgg atcacgaaaa tgatcagtta
     1201 catcagcagg tttatgaata agaacattct cacctttcgt aatgttgtag tctttatcca
     1261 tccatgctcc gatttgaagt gtatcacgtt cccaattttc atttcgaacg tttcctgtat
     1321 agagtaagaa tagattcccc tctatttcat atgcagaacc ggaataagcg ccgtggctat
     1381 ctcgttttgt gtctggaaga agttctgctc ctgttacatg aaaatgaact aaatcatctg
     1441 attcattgtg tacccattct ttcaacccat gagcagctcc aaatggccaa ttttgataaa
     1501 aaagcgtata ttttccgtta aaaaaagaaa aaccattagg atcgtttaac aatcctgttt
     1561 taggttcgat atgatagcta gcacgccaag gtgatcgttt tacattatcc ttaattttat
     1621 ccatctcatc tttagtccaa tcagaataag cacgatatcg tatttctcga ggtaagttca
     1681 tattttactc cttcaattta tctttttaca actagtatgg taaacgtttt ccgttgaaaa
     1741 atcaatcatt tcatttaaaa aaataaacta tatgacaaac gtttgacata tttttaagat
     1801 atgctaaaat aaatttgcat ttcagtgaac cgttttcacg taaaaataaa aaaggagtga
     1861 ttcgaatgaa ttacagtaaa gtagcttccg acgtaataca agcggtcgga aaagataacc
     1921 ttattgcagc tgcacactgc gctacacgtc ttcgtctcgt actaaaagat gacacgaaag
     1981 tgaatcaaaa agctttagac gaaaatccag atgtgaaaag cacatttaaa attgatggac
     2041 aatatcaagt gattatcggt gcaggtgatg ttaatttcgt ttatgacgaa cttatcaaaa
     2101 aaactggact ttcggaaatt tcaacagatg atttaaaaca aattgctagt aaagataaaa
     2161 aattcaaccc cattatggca ttaattaaat tattatcaga tatttttgtt ccaattatac
     2221 ctgcccttgt tgcaggtggt ttattaatgg cattacaaaa ctttttaaca gctgctggac
     2281 tttttggacc aaaatcaatt gaagaaatgt atcctgcgat tgaaggtatt tcctcaatga
     2341 tccaactgat gtccgctgca ccattcatgt tcttaccaat tttagtaggt atttcagctg
     2401 ctaaacgttt tggagcaaac caatttcttg gtgctgcaat tggtatgatc atgacaacac
     2461 cagatcttgg tggctcaact gaatattgga atatttttgg ttatcatgtt tcacaaacaa
     2521 actatgctta tcaagttatt ccagttttag cagctgtttg gatattatct atattagaaa
     2581 aattctttca taaaaaattg ccatcatcta ttgactttac attcacgcca ttattatcag
     2641 taatgattac tggatttttg actttcacag ttattggccc tgtaatgtta ttgttgtcta
     2701 atggaattac agacattatt gtttggctat ataacactac tggatttatt ggaatgggta
     2761 tttttggagg aacatattca cttatagtca tgactggttt acatcaatca tttccagcta
     2821 ttgaaactca attactttca gcgtggacaa atggtatcgg acatggagac tttatctttg
     2881 tagttgcatc tatggcaaac gttgctcaag gtgctgcaac atttgccatt tggttcttaa
     2941 ctaaaaattc taagacaaaa agtcttgctt cctctgcggg tgtctcagca cttcttggaa
     3001 ttacagagcc tgccttgttc ggagttaact taaaataccg ctttccattc ttttgtgctt
     3061 taattggttc aggaattgct acagcagtag caggattact taaagtagtt gctgtttcac
     3121 ttggttcagc aggtttcttg ggatttcttt caattaatgc aacatctatt ccattttacg
     3181 tattgtgtga attgattagc tttgtagttg cttttgcaat tacttacttc tacggtaaaa
     3241 caaaagctgc tggtatcttt aaagcagaag aaattgctga acaaacacca gttaattctt
     3301 caaaagtagc aacaacagat ccaatatcta ctgatgttga atcaacacaa acaaaagttt
     3361 catcagaaac aattgttagt cccatttctg gtaaagtgat tagtctttct tcagtaaatg
     3421 atccagtctt ttcatcagaa gctatgggaa aagggattgc cattaaacca aatggaaaca
     3481 cggtccattc tccagtagat ggtactattc aactcacgtt tgaaactgga catgcttatg
     3541 gtattaaatc aaataatggt gctgaagttc taattcatat tggtattgat actgtatcaa
     3601 tgggaggaaa aggatttaca caaaaagtta ctactaatca aaaagttaga aaaggcgata
     3661 ttcttggagc atttgaccgt tcaatgattg agaaagaagg tcttgatgat acaacaatga
     3721 ttattgtgac caatacttcg gattattcag aagtaaatat agtagctgaa aatacagtca
     3781 ctgagggtac tgatttactt gaaatcaaat aaatatattt ccctgagtaa agatggaatt
     3841 ttctttccaa aatcaaataa tatagattaa tcaaatat
//
LOCUS       DAYYHX010000213         3864 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000213 DAYYHX010000000
VERSION     DAYYHX010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.215"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(413..679)
                     /locus_tag="ACHA6Y_002537"
     CDS             complement(413..679)
                     /locus_tag="ACHA6Y_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988598.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            complement(690..881)
                     /locus_tag="ACHA6Y_002538"
     CDS             complement(690..881)
                     /locus_tag="ACHA6Y_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988599.1"
                     /translation="MSNLDNGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHW
                     SGPKANRTILEIIRDYRIR"
     gene            1172..1594
                     /locus_tag="ACHA6Y_002539"
     CDS             1172..1594
                     /locus_tag="ACHA6Y_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8988600.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            1611..2033
                     /locus_tag="ACHA6Y_002540"
     CDS             1611..2033
                     /locus_tag="ACHA6Y_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8988601.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            2062..2262
                     /locus_tag="ACHA6Y_002541"
     CDS             2062..2262
                     /locus_tag="ACHA6Y_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988602.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            2349..2798
                     /locus_tag="ACHA6Y_002542"
     CDS             2349..2798
                     /locus_tag="ACHA6Y_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HFM8988603.1"
                     /translation="MKKVITSLLVFASLLLFTACGKKVTTEDLKANDWLIEATKDDEP
                     NMIVSFSDHVVSFSVDTSSMKSTSSNDWEAMGEEFAKSLVDQMNYKFEYTLEGDVMTW
                     KDDKDKDNDAKYTVSKEDKNIILTPEKPNKSDDNEKLVLKPYKKEKN"
     gene            3106..3237
                     /locus_tag="ACHA6Y_002543"
     CDS             3106..3237
                     /locus_tag="ACHA6Y_002543"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8988604.1"
                     /translation="MQPGEGTKQIAERAGITVDQLFALNGMDPNNYMLYPGQELRVK"
     gene            3375..>3864
                     /locus_tag="ACHA6Y_002544"
     CDS             3375..>3864
                     /locus_tag="ACHA6Y_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFM8988605.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITS"
BASE COUNT         1433 a          669 c          642 g         1120 t
ORIGIN      
        1 tatcattcca atgacattga cgttttctaa agagcaattt aacgtagcta ctacattcgt
       61 atcttttata gctctatttt cgtttgctgt tcctaaaact tttcaaagca aatctagcaa
      121 cgcaaaggtt aatgtagaaa acactgatag aaaaaatgat gaaagttatt aaaaaaaata
      181 attctttcat ttattgtcaa acctttctta tagacattag acttttttag tcagtccctc
      241 ccgactggct ttttcgctct gtactcagct tcatcaagcc caatgaaaat ccaaagcatg
      301 taaacgatcg tgccgattaa tgcttgtcta ctaccccata gacctaaagc gtagataatt
      361 agtggcgcgc tgaatactaa tgttcgatta aactttccca tgttgacctc ctttaaattt
      421 tgtatttagc catcagttca tcgatgtcct ttttgtcata tcttaaaaag ccatctatac
      481 gaatttcttt taaaccgtga gctattaact tctcaaaacc ttgatcgttt acacctccca
      541 catattttct agcttcgatc ttttttaaat atcgtgtttc tgacaaattc ttttttgaaa
      601 gttgactaag tgcgtccttt acaacttcaa caattgcaat tcttaaacga gtcaaaaact
      661 cttcgcttag aatattcatg acttgcctcc tatcgaattc tgtaatcacg gatgatctct
      721 agaatagttc tgtttgcctt tggtccactc cagtgtccat caagaatttg ttgcattcgt
      781 acacgcgtgt gaccataagc agtagccaaa tcatccatag ttattccatt ctctttcata
      841 aactctttga tcgccgcacg accgttatcc aagtttgaca ttaacccact tccttttttt
      901 ataaatttgt aagttaaaat gatagaaaaa atataatttc cattgacatt tttacactat
      961 agagtaaaat gaatacatag agaaataagc ctataacaaa accttttatt tgcacttggt
     1021 cgccaaactt tatgctataa ggtgtgtttt tagtttgctt ttttctatca aattaactta
     1081 cggataaata ataactctat atcgtaaaat tgtaaagcta tttttactct ttttcgtaaa
     1141 ttattttttc gtgttggaaa ggaacgttgg aatgacaaca tttgaaagaa taaaagaact
     1201 agcgaaaaac aagaataaaa accttcaaca agtcgctaaa gatttgaagt acggagagaa
     1261 ttacttttat agtttaaacg caggaaaaca gccaactgcc gaaaagttac aagaaattgc
     1321 tgactacttc gacgtatctg tagattattt gctaggacgt actgagaaaa aaagatacta
     1381 tgatttaaca gaaaaagacg agcgtgatat ccaaaaagaa ttgcagtcat taatcgacga
     1441 tttatctaat gctgatggaa tggctttttc gaagaaagat aatgaaatga gtgaagcgac
     1501 acgtgaagct ctcattattt ctttagagaa tgctttgcga atagctaaaa tagaagctaa
     1561 gaaaaaatac acacctaaaa aatatcgcga ttaaaaggag tacgttgttt atgtatttac
     1621 ctcaaattga taacaaaatt aataagctgg taaaactata tcaaacacgc aatcctttta
     1681 gaattgcacg agaattgaag atactagttt tagaagaaga tttgggagaa atttatggtt
     1741 actatagtaa attcaaacag ataaaaatga tccacataaa ttctagttta gatgagatgg
     1801 gaaaatggat aacttgtagt catgaattag gccactgtgt tttgcatccg aatgaaaaca
     1861 cacctatgtt gtctaaaaaa acgctagtat ctgaattaaa aattgaaaaa gaagcaaatt
     1921 atttcgcaac tcatctgact attgatccaa ctttagacgg ctttgatcat atgacaaaat
     1981 atgaaaagct cgtctgcttt ggattaccag atgaattcga tcgtttcttg tgaattataa
     2041 tacaacgtaa ggaggatata gatgaaaata ggaatgagaa aaccaagtat aaaaaaatcc
     2101 ataagcgcta gaaccactgg aaaagcaaaa agaaaagtaa aaaaagcagt tattcctggt
     2161 tatgggaaaa aaggtagcgg ctggattaaa gacccaaaaa aggctgctta caataaagta
     2221 tacaagaaaa ctacattcag tttttgggat ttatttaaat aaaaaaagcc cgtgcagcaa
     2281 cacggactcg aaaccttatt tctaagattc cccaataaaa atcatatcat agaaatgagg
     2341 aataaaaaat gaaaaaagta ataacaagtt tattagtttt tgcttctctg ttacttttca
     2401 ctgcttgtgg taaaaaagta actacagaag atttaaaagc taatgattgg ttgattgaag
     2461 caaccaaaga tgacgaacct aatatgatcg tatcattttc tgatcacgtc gtatctttta
     2521 gtgttgacac aagttctatg aaatcaactt caagtaacga ttgggaggct atgggagaag
     2581 aatttgctaa aagcttagtc gaccaaatga attacaaatt tgagtataca cttgaaggcg
     2641 atgttatgac ttggaaagat gataaagaca aagataatga tgccaaatat actgtcagca
     2701 aagaagataa aaatattatt ttaactccag aaaagcctaa caaatctgat gataatgaga
     2761 aattagtttt gaaaccctac aaaaaggaaa aaaactaaag aatctagaac aactactagc
     2821 tcaacaagta gcaacgaaca atcatcttcg actacaacag aaagtactat tgcatcttct
     2881 agtactgtag aacaagctgc tattgcgcag aatacgcctc ctgaaactag tagtacttac
     2941 tctgctgaaa gtgtagaaaa ctatgcagaa gctactccta gttcaagtgt tgaacaagtt
     3001 tcacccactc ccagttcaac ggagcaatca gtaccacaag attatgtacc agctaatcaa
     3061 gctcaaccaa cggcaccttc ttcatctgaa accgaaacct tgactttgca acctggtgaa
     3121 gggaccaaac aaatagctga acgagctgga attacggtag atcaactgtt tgcattaaac
     3181 ggtatggatc caaacaacta catgctttat ccaggacaag aactaagagt gaaataaaaa
     3241 aagcacattt gccgcccgac caagaacgca aatgtgcaaa gagctacggt aaagtaggtc
     3301 tatttgttgt accctatttt accatctcga aatccgctgt acaaccgaac aaacgtacga
     3361 aaggaaagat aaagatggca aaaaaagaaa ttgataaacg aattaaaaag cacttaacaa
     3421 aaaaaggcga agaaaaatat cagtttagtc tgtacttagg agttgatcct ctaacaggaa
     3481 aaaaaagaaa aacaacgaga cgaggtttta gtacgccatt agccgctgag agagctttaa
     3541 aaagattgga ggcagatata caggaaaaag gactgcaagc ttctacagct caaaagagca
     3601 agaaattcga atatgtttac ggcttatggt ttgaaaatta taaaaagaca gttaaagaaa
     3661 gcacttggtc ctccaccaaa caaatttttg acacacacat cttaaaagtg tttggagata
     3721 agttcattga caaaatagac gtttttttct gtcaagaagc agtaaacact tggtccgata
     3781 gccatcctaa aatttttaag aaaataaaaa attacacatc taatgttttt gattacgctg
     3841 cttctttaca aattataact agca
//
LOCUS       DAYYHX010000214         3848 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000214 DAYYHX010000000
VERSION     DAYYHX010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.216"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(3..1157)
                     /locus_tag="ACHA6Y_002545"
     CDS             complement(3..1157)
                     /locus_tag="ACHA6Y_002545"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM8988606.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1342..3783
                     /locus_tag="ACHA6Y_002546"
     CDS             1342..3783
                     /locus_tag="ACHA6Y_002546"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM8988607.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         1232 a          721 c          840 g         1055 t
ORIGIN      
        1 tattatattt tgtagtaacg aatatcattt tcgatctttg taatcgaaaa aggtacatct
       61 gaagtatatt ctgcacattc tccgtgttca ggaagcgtct cactgaaaat cgtttcatat
      121 tcgtcaacag tgatccgtgt tcgagaatct agcatctctt gatgagattg tgcgaagaga
      181 tattcttgat agttttcttc cagataacct gtaaaaaatt cacttactgc accagaaccg
      241 tagctgaata gaccgatgcg atctcctggc tgtagtgatt tcgaattttc cagtagagaa
      301 gttagcccca ggtataatga accagtgtaa agattaccga ttcgtcggct gtaacggatg
      361 ctttcttcat agcgagccat aagacgttcc tgattttctt cgtctgtttg gtctaatacg
      421 ctttgcaatg ccttttttcc catcttagta tacggaatat ggaaagcaat cgcctgataa
      481 tcttcgagtc ctcgacccga caattcttta tgtcgattcc aaactttttg gaatgattcg
      541 atatacgtag aattagataa aggaccatca acaacaggaa attcgctata atctggacgc
      601 cagaaatcat agatatcttc tgtcagaaat acgctgtcgt cttcaatcga taaaatacgc
      661 gggttttgag tgatcatcat cgcaacagca ccgacacctt gcgtcacttc accaccgctt
      721 gccaagctgt aacgagcaat atcacttgct atgactagta cttttcgttc tggatgattt
      781 ttgacatatt ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc ctcttttact
      841 tcaaaagaac gagcaaaaga ttggattttc agtaaatggt ggataattac cgcactcgct
      901 tttgaatgat caatcccgga ttctgttccg acgatgacca tgtcgattag ctcacggtct
      961 ttttctgtta cgattttgct ggcagcattt gctcctagtg taatgatgtc ttcatttgca
     1021 cgattgactg ccatttggtc ttgaccaatc cctatatggt attttgcagg atcatcccca
     1081 cggctttctg ccaactctgt catatctaaa tataaattag gaataaaaaa ggaaagacga
     1141 tcaatcccta ttttcatttc aacacctctt tacttatgat aaaaaactta ctttactaag
     1201 gtatcataaa aagattaaaa gaaatataaa aaagagaata tttaaggatt gttaaaattt
     1261 aatcttagaa aaaaataact ggaacaagag ctatttgttt ttacagcaaa gaatggtata
     1321 tttttgtagg aggtggctta cttgaaagaa gtcgttatga tagatgctgc aagaacgccg
     1381 ataggaaagt atcgcggaag tttaagtcca tttactgcag ttgaattagg cacacttgtg
     1441 actaaaggat tattagataa aacaaaactg aaaaaagata aaatcgatca agtaatattt
     1501 ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca gacagattgc tttgaacagc
     1561 ggtctccctg tagatgtccc agcaatgacg atcaatgaag tttgcggatc tggtatgaaa
     1621 gcagtgatct tagcacgcca gctgatccaa ttaggtgaag cagaacttgt gatagccggc
     1681 ggaacagaga gtatgtctca agcacctatg ctgaaacagt atcagtcaga aacaaatgaa
     1741 tatggtgaac caatttccag tatggtcaac gacggattga ctgacgcatt ttcaaatgca
     1801 catatgggat taaccgcaga gaatgttgca acacaatttt ctgtgagcag agaagagcag
     1861 gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt
     1921 tctgaggaga tcatcccagt caagatttct gatgaagacg tgttatctga ggatgaagca
     1981 gttcgtggaa atagtacatt ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa
     2041 ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg gtgcctctgt ggtgatcctt
     2101 gcatccaaag aatacgcaga aaataataat ctgccttatt tagcaaccat caaagaagta
     2161 gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa
     2221 ttgacagata gatcaggaat gaatttatca acaatcgatc tttttgagat caatgaagca
     2281 tttgcagctt cttctatcgt agttagccaa gaactgcagt tagatgaaga aaaggtcaat
     2341 atatatggag gagcaatcgc attaggccat ccaattggtg caagcggtgc acgtatcttg
     2401 actacattag cttacggatt gcttcgtgaa cagaagagat acggtattgc ttcattatgt
     2461 attggtggag gtttaggctt ggcagtcttg ttagaagcga atatggagca aacacataag
     2521 gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg aacggcgcag tcaattaata
     2581 gaaaaaaatg tattgacaca agaaactgcg ttgatttttc aggaacaaac actttctgaa
     2641 gagctctccg atcacatgat cgaaaaccaa gtgagtgaag tggaaatccc aatggggatc
     2701 gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc cgatggcaac agaggaacca
     2761 tctgtcattg cagctgcaag taatggtgca aaaatctgtg gaaatatctg cgcagaaaca
     2821 cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa aatcggagta tcaagcagtg
     2881 atcaatgcag ttaatcatcg aaaagaagag ctgattcttt gtgcaaatga aagttatcct
     2941 tcaatcgtga aacgcggggg cggtgttcaa gatatctcta ctcgggaatt catggggagt
     3001 ttccatgcat acttatctat tgatttttta gtggatgtaa aagatgcaat gggcgctaac
     3061 atgatcaact cgattcttga gagtgtggca aacaagttac gggagtggtt ccctgaagaa
     3121 gagattctat tcagtatttt aagtaatttt gctacagaat cattggcttc tgcctgttgt
     3181 gaaatcccat tcgaacgctt agggagaaac aaagaaatcg gtgaacaaat tgccaaaaaa
     3241 atccaacagg caggggaata tgcgaagctt gacccttacc gtgcagcaac gcacaacaaa
     3301 gggatcatga atggtataga agctgtagtg gcagctacag gaaatgatac acgtgcggtt
     3361 agcgcttcga tccacgccta cgcggcaaga aatggattat accaaggatt aacagactgg
     3421 cagatcaaag gggataaact agttggaaaa ctaacggtgc ctttggctgt tgccacagta
     3481 ggcggagctt ccaatatcct gccaaaagca aaagcatctt tagctatgtt agatattgat
     3541 tcagccaagg aactagcaca agtaattgct gcagtagggt tagctcaaaa cctggctgct
     3601 ttgcgtgcat tagttacaga aggaatccaa aaaggccaca tgggacttca agctcgttca
     3661 ttagcgatct caattggggc tattggtgag gaaatcgagc aagttgcgaa aaaattacgt
     3721 gaagcagaaa agatgaacca gcagacagct atacaaatat tggaaaaaat ccgggaaaaa
     3781 taaaaaagca atatctttag aagaataaag caagatttac agttatttta tttactgcag
     3841 atcttgct
//
LOCUS       DAYYHX010000215         3829 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000215 DAYYHX010000000
VERSION     DAYYHX010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.217"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..357
                     /locus_tag="ACHA6Y_002547"
     CDS             <1..357
                     /locus_tag="ACHA6Y_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8988608.1"
                     /translation="NVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCI
                     CGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGI
                     KLNQKTIKKLNIGGEK"
     gene            354..1103
                     /locus_tag="ACHA6Y_002548"
     CDS             354..1103
                     /locus_tag="ACHA6Y_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8988609.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1115..1384
                     /locus_tag="ACHA6Y_002549"
     CDS             1115..1384
                     /locus_tag="ACHA6Y_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988610.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            1390..1554
                     /locus_tag="ACHA6Y_002550"
     CDS             1390..1554
                     /locus_tag="ACHA6Y_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988611.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1547..1846
                     /locus_tag="ACHA6Y_002551"
     CDS             1547..1846
                     /locus_tag="ACHA6Y_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988612.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            1846..2148
                     /locus_tag="ACHA6Y_002552"
     CDS             1846..2148
                     /locus_tag="ACHA6Y_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8988613.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            2188..2553
                     /locus_tag="ACHA6Y_002553"
     CDS             2188..2553
                     /locus_tag="ACHA6Y_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988614.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            3043..3183
                     /locus_tag="ACHA6Y_002554"
     CDS             3043..3183
                     /locus_tag="ACHA6Y_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988615.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            3180..3362
                     /locus_tag="ACHA6Y_002555"
     CDS             3180..3362
                     /locus_tag="ACHA6Y_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988616.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            3375..3569
                     /locus_tag="ACHA6Y_002556"
     CDS             3375..3569
                     /locus_tag="ACHA6Y_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988617.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(3602..3814)
                     /locus_tag="ACHA6Y_002557"
     CDS             complement(3602..3814)
                     /locus_tag="ACHA6Y_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988618.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
BASE COUNT         1406 a          599 c          813 g         1011 t
ORIGIN      
        1 aatgtcttag caaatatcat cttagatttc atctttgaac atcatattcc tttcaaagaa
       61 ggctatgaga ttttacctgc gaatcaagaa tattacttct acaaatgcat cacaaaaaga
      121 gtctgctgca tctgtggcaa aacaggagct gacatcgatc actttgacaa agcgctagga
      181 agacgaaagc gcaaagaagt tgatcatgca gagtacacat ttgcagcact ctgcagaatc
      241 catcacacag agaagcacaa aataggtgtg attaatttca aaaataaata tcaaatcaaa
      301 gggatcaaat taaaccagaa aacaatcaaa aagttaaata ttggagggga aaaatgacag
      361 aacatcgaag ttattatgcg attataccag ccaacgtaag gtacgacaaa agacttaaac
      421 caaatactaa gttgttatac ggagagataa cggccttgtg taatgaaaga ggcttttgtt
      481 gggcaggcaa tgagtacttt gcagatttat atggtgtgaa taaagagacc atatcgcgat
      541 gggtaagtga tttgattaag tttggatact tgaatcggga aatcatttac aaagagggta
      601 ccaatcaaat aatcaatagg tacctacgaa ttaatcaata ccctattgac gaaaaacgca
      661 atacccctat tgacgaaaaa gtcaaagata ataatacatc tattaataat acatttaata
      721 atacaaaaga atatataaga gagttaccgc cttcgaaaaa atcgaaggct aagcccgtcc
      781 gtcataaata cggagagtat aaaaatgttc ttttgtcaga tgagcagatg gagaaactca
      841 aaacagaatt ccctaatgat taccaagagc gaatcgaacg actgtcagag tattgtgaat
      901 catctggtaa gacttataaa aactatttgg caactattcg aagttgggca aggaaagaaa
      961 aaagtgaacc taagaacgca agcagtggat acaagcgcac aggaagacga gagaagcttc
     1021 ctgaatgggc aatcgaccaa gaagcctatc tcaagaaaaa agcgctagaa cgagctaata
     1081 gacaatcaaa agcaccattc taagaggtgg aaaaatgaag atcgattatc tagaactaat
     1141 taatgaaata gcgaattata aaaagggcga ggaattagac gtcctgagag acgtatatga
     1201 tcaactcgaa gaagctggaa ttgaaggaat taagaatgat cgttcgagtt ggagtaaact
     1261 cagatactat ttcgcactct atatcgatgg aacgcaatta agaaatttag catataccaa
     1321 attactattc attgattgtg ttaaaggctt gcaaaaacat cttaatgaac ttgagcaggt
     1381 gtaaacaaga tggacctaaa gacatttaca gcacagatcg aattaatgca tcaagaagct
     1441 ttaagacaaa gtgtgtcgta cgaagacaag tggctcaaca catttcatgg cggacgtgag
     1501 agcgcacttg atcaagtact caaattactg aaaggagaat gtcgggatgg ataagaaagc
     1561 ggcaatgcag cgaattatcg aattgactta ttcagaagat tggcaggaag ataaaaaaat
     1621 agttgcagaa gtccaaaagc tcggtaaatc aatgtggact gaaaagccca aacggagaac
     1681 gccgagaaag attgcaatct ggcatggtga tcgaattcta gtaacgggta ccgctaaaca
     1741 gttatctgaa attactggtc tgagcaaaaa tattatttgg gatagagcta ggagcttatg
     1801 gattgattca aaaggaagac agtttaggta tgtggaggag aaataatgga cgaactaatc
     1861 gcgaaagtag agcagtgggc aaaagataag ggattggatc aagcggattt cagcaagcaa
     1921 atgttaaaaa cggtcgaaga aatcggggaa gttgccgctt ctctagctag aaaagatgaa
     1981 catggtttaa gagacggaat tggcgatgta gtagtaacct tgatcatctt ggcaatgcaa
     2041 aatgatatgg atttatacga gtgcttggat tttgcttatg acgaaatcaa aggtcggact
     2101 ggaaagatgg tcaatggtgt tttcgttaag tccagtgacc tgaaataatt ccattaatgg
     2161 aagaaataac tgaataggag gatagacatg attgatgcaa aacaagtgca aaaacagaaa
     2221 gatggaatgt tgatgtttga agcatatgtt ctaccatttc ttaatcaatt tgaagttttg
     2281 gaatgctctg ccagcggaga agaattggag tatgttgtaa ttcgtgaaac caaagaaaac
     2341 gttcagaagc taaacgaatt tctttgcact attaactgtt gggatatgat agctccagga
     2401 tttttatgtc cagctatggg agaatttttg gaatattgtc gtcttgaaga tgcaggcact
     2461 ttggatttag cttatctggt atacaactac ctaaacatca acacagatca tttgtggttt
     2521 ggcactgccg aaagaaaatg ggtagtccgc taaccaccaa tatcaatgaa aaaaggcggt
     2581 ttcctgaaag tggaattgcc gccattgctg taaggttctt tacgcgactc aaggtcacag
     2641 aaatataaat gcaaatctct tttgaagtga tccaaagctc tcggaaaatg gaagtttttt
     2701 gtttcaaaga ataaatgaga tttgcgtcaa gaacctgaga gttatttcca aaaaagcttc
     2761 caaagaaccg aatgagctaa ggacatcatg tgtaaacaca taatatagct ctcctttcac
     2821 ggctaatcat aacatgaaaa atattcgatg tcagtaggaa aaaaggtgaa ttatgaaaag
     2881 aatattaaat tatcccggca gtaacagatt ctgcaattaa actccgaagc tgatgtatca
     2941 gtatcaactg tagaatatta tgagcgtgga gaaagtcaag cagtcacaaa tgtaaaaatc
     3001 gataacgtca acggtcttat ctgtttagat gaaggcgata gtatgaatga aattgctgca
     3061 gaccatgaat tatcagaaac tgctttaatt gatcaaatcg aattagctag acaatacttt
     3121 gttccatatg cagatacctg gtccaagcat aagaatgaca tcatttttcc agaggaacaa
     3181 tgagagcaac tgatccagta attatccttg aggaagccaa atttatttgg actcacgaag
     3241 agatagagca agcacgcttg ctcttttctc aaggagttaa gccgagcaaa gtagctgaaa
     3301 taatgggtca aaagattctt gatgtcggat tgcttttgct ccatctagca gaaaaaattt
     3361 gatttgaggt gaaaatgatg attcaacttg caggcatcca aacagggaaa atttattttt
     3421 ctggagaaag caaaagcgaa gccaaccaat ggctattgaa aacttacacg aacaataaga
     3481 agctaaggaa aaaatatcct gatttgttgc taaaggatga tcagattatg ccggaaccga
     3541 tgattttgac ccgtaaggag ggaagttgaa actaaaaaac cagccgtgtg ggctggagtt
     3601 gctatttctt tttaataatg agtttttcta aatttaattc attctcacga gtttgcgtcc
     3661 aataatcaga ataatatttt aattgtttaa gagtaggaat gtcgtcttcg gtaaaattag
     3721 aaaataattc tggagaatta tcttttttta attgttctaa tcctgattct ttaaaacgtt
     3781 gttcaacgag ttttactaaa ttgttgatat tcatgataaa tcacctttt
//
LOCUS       DAYYHX010000216         3805 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000216 DAYYHX010000000
VERSION     DAYYHX010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.218"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(176..469)
                     /gene="rpmA"
                     /locus_tag="ACHA6Y_002558"
     CDS             complement(176..469)
                     /gene="rpmA"
                     /locus_tag="ACHA6Y_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM8988619.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(482..826)
                     /locus_tag="ACHA6Y_002559"
     CDS             complement(482..826)
                     /locus_tag="ACHA6Y_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM8988620.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(853..1161)
                     /gene="rplU"
                     /locus_tag="ACHA6Y_002560"
     CDS             complement(853..1161)
                     /gene="rplU"
                     /locus_tag="ACHA6Y_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM8988621.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(1181..1257)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            1443..2084
                     /locus_tag="ACHA6Y_002561"
     CDS             1443..2084
                     /locus_tag="ACHA6Y_002561"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8988622.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(2146..3777)
                     /locus_tag="ACHA6Y_002562"
     CDS             complement(2146..3777)
                     /locus_tag="ACHA6Y_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM8988623.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT         1175 a          842 c          669 g         1119 t
ORIGIN      
        1 cttccatcaa ttgaaaacga tagttatcaa aacatcctga ttccaaattg gcaaaaaaat
       61 attaagtgta aaaagacaaa gtaaacttcg tagcctccac tacaactttt atttttataa
      121 acaaaaaagg ctgtgacaaa agtcttgtca caacctcttt aatcataaga ttcaattaag
      181 cttcttgagc tactggataa acagacactt gttttttgtt acggccttta cgttcgaaac
      241 gtactacgcc atcaactttt gcaaataaag tgtcgtctcc accgataccc acgtttacac
      301 ctggatagat ttttgtaccg cgttgacggt aaagaattga tccacctgta actgtttgtc
      361 catcagcacg tttagcacct agacgttttg attctgagtc acggccgtta gatgtagaac
      421 cgccaccttt tttgtgggca aataattgta gattcataga taacaacatg gtctgcacct
      481 cctatttatc attaattgtt ttaatttgga tatattctga gttttcagct tctatcgatt
      541 gtaatcctaa taaaagattt tctaatagaa tctcagcaat attgttttgt tcttgtgtca
      601 ttcctgaagt gacttcggta taaagataac cgccttcttc ttcattcatt tcaacaatcg
      661 gctcaaaacc ggctaatgag gcaatcccgt tgacagtgct gatcgctaac gcagaaacac
      721 ccgcacaaac gatatcactt ccataaggac cagcatcagc atgcccggtc aaagtgaatg
      781 aaacgatctg acctgcgtca tttcgcttaa atgttccttt gatcatatat aggccttctt
      841 tctcaaacgc tattaagcgt tgatcgcgtc gataaccact tttgtatatg gttgacgatg
      901 accttgttta cggtgtgaat gttttttagg tttgtatttg aaagtaacga ctttcttttg
      961 tttgccgtgt ttttcaacag taccttcaac agttgcacct gcaacagttg gagcgcctac
     1021 ttttgtagat tctccgccta ctaagataac ttcgtcaaaa acaacttttt ctcctgcttc
     1081 aacgtcaagt ttttcaacat agattgcttg tcccacttca acttttactt gtttaccacc
     1141 agttttaata attgcgtaca tgctataaag cacctccttc tgatatactt agactcgcca
     1201 tccgaagtga cagcatatgc tgtgcttatt acttgttctg agcggttgta gctgtggagt
     1261 ccacaattac aacagtacta gcttaccaga acagcaaccg ttagtcaact aaatttaaaa
     1321 tcaaaaagta aaacaaaaat tcaagcaata cttctgcttt tctatcctca cagttctacc
     1381 aaataaaata gtcgcattgt gtttctttta ttcttataat aaaagaaaaa ggaggagcat
     1441 aaatggaata cccaaaagaa ctgatacaag aagcttcttt aagaatgaac ggccgtcctg
     1501 tagaaggatt tatcgctggg caaggacctc ttcatcctaa actgatgctc gtgggagaag
     1561 ccccagggaa aacagagatt gatacgcatg ttccttttag cggtcaagct ggaaaagaat
     1621 tgatgcaggc actttcttca actggcttaa cacgagaaga ggtgtatatc accagtgctg
     1681 taaggagccg cccgtatcgc gtgactcatc ggatcaataa acggacacaa cagtcagaaa
     1741 ctgtttatcc aaatcggaca cctactcgtt ccgaagtatt tgcgcatgca ccaatattgg
     1801 attacgaact gatgaaggtc cagccaaaat taattgcgac acttggaaac attggtcttc
     1861 aacgtttact tggcaaagaa tatacggtta ccaaaaatca tggtcaactc tatacagggc
     1921 ctgtcttaca attaacagaa caaaaggatg cctacgaatg gtcagaaaaa aactatcgaa
     1981 ttcttccact ttttcatcca gcagctattt tctacaatcg ctcccttgta cctgttatcc
     2041 atcaagactg gcaagtacta gcttcacttt tacagcaata ttaaaaagaa cagacaagac
     2101 cataaaatct tcagtttaca gtcttgtctg ttctttttca ttgttttaat ggctaattgc
     2161 agtatatttc gcatgtaaag ctgtcaacgc atgagcaact gtttgatgtt gatgcgtgaa
     2221 tgtatgttcc cacaagtgag aaagttgttt ttctacttct ggctgcaagg aagtatagat
     2281 aatcgtttgt cctctttgct ctgctttttc ttgaaattca tcaaaaactg cacgtgccgt
     2341 caaatcaata cttggtacgc ctcgcatgga gaaaatgaca ccatcgtgat gatttagttc
     2401 ttccaattta tttttgagtt tttctgaaga cataaaaaac agtggaccac taatatacac
     2461 tactgtccag ttattcagct tttccgtttc tggcagcccc attctttgcc aatcgatcgt
     2521 ttctaatgaa atcgtgatag cagcgctttt gacaacaaaa aatacacagc cgccgatcac
     2581 cccgatcacg atagcaatac ttaaatcaaa gataactgta caaatcatcg taaggataaa
     2641 caatagaact gctgaccaat atttattagt aaatagctcc ttgatcgttt cccattcatt
     2701 catacgccaa gccgtcacaa tcaaaacacc tgcaagtgct ggcattggaa tctgtgacat
     2761 cactggcgca aaaacaagca tggagagcag taagacaact gcatgtatca taccagctaa
     2821 tctagtttgt gcaccagatt tgatggccac gcttgtacgg gcgatggccg cagtagcagg
     2881 aatcccgcca aagaaaggaa gcaacagatt tccaattcct tgcgccatta attcttgatt
     2941 gctatctaag ggcttatttg ccattcggcc agccgaagca ccacatagca agctttcaat
     3001 cattcctaat aaagcaatgc taatagctgg aactgccact tgttgcaagg cagaaaagct
     3061 aaaatctccc atattcaaac gattactact gatcaatgtt tgtggaatct tacccacagt
     3121 tgctactggt aaatggaaca atagcataac tgctgttgcc aaaatgatag ccagcagcga
     3181 actaggcatt cgtttcgccc atttttttgg atagataaac atccctaaaa tgactagccc
     3241 tcccatgatc aaagaggctg tgtttatcgg aaatcctatc gtttgataac tacttagttt
     3301 ttcaataacg tttgtacctt ccgagtgaac accaaataaa ttatcgatct gacctaatgc
     3361 aataatgata gcaatacctg aagtaaaccc cgtaattacg ggtgctggga taaaagaagt
     3421 cagtgttcct aatcggaaaa gtcctgctgc aagtaaaaat agacccgcta aaaaagtagc
     3481 aagcaataca ccttgcattc cctttgttgc cgcaatagac atcaagatag ctgccatcgc
     3541 acctgtcgga cctgatattt gataaaaacc gcctgataaa ccgccaataa cgataccggc
     3601 aatgatagcc gtaatcaaac ctgcggccgc atcagctccg cttgataccc caaaagccaa
     3661 ggctaaaggc aatgctacag cagctactgt gatcccagcc aaaagatctt tctgaaatct
     3721 tgaaagatca taacctgaaa attctttttt tagtgaggaa acataatttt tgaacaccta
     3781 tctaactcct ttttcatgaa atttt
//
LOCUS       DAYYHX010000217         3787 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000217 DAYYHX010000000
VERSION     DAYYHX010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.219"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..2060)
                     /gene="tkt"
                     /locus_tag="ACHA6Y_002563"
     CDS             complement(63..2060)
                     /gene="tkt"
                     /locus_tag="ACHA6Y_002563"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM8988624.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2162..2413)
                     /locus_tag="ACHA6Y_002564"
     CDS             complement(2162..2413)
                     /locus_tag="ACHA6Y_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM8988625.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2560..3183
                     /gene="lexA"
                     /locus_tag="ACHA6Y_002565"
     CDS             2560..3183
                     /gene="lexA"
                     /locus_tag="ACHA6Y_002565"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM8988626.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3363..3581
                     /locus_tag="ACHA6Y_002566"
     CDS             3363..3581
                     /locus_tag="ACHA6Y_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988627.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1039 a          808 c          708 g         1232 t
ORIGIN      
        1 taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac aattgcttgg
       61 atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc aaattcttct
      121 aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct
      181 gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac acgtttcttc
      241 actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa gaggtcaaag
      301 cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt ttgagctttc
      361 acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt ttctccttgt
      421 gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc ttttgtagaa
      481 ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc tgttgtctcc
      541 atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat cacttgaaca
      601 ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct
      661 ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg gattgctgct
      721 aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc gtagatacga
      781 gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc gcggacaccg
      841 aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc agccaccatt
      901 gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat agctttcgat
      961 aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc aacttcataa
     1021 gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc ttcaaattgt
     1081 tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca tgcatcttca
     1141 gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc ttctggaaca
     1201 gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc atcttcacca
     1261 agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc gatgactgtt
     1321 tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat cgctttagag
     1381 attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata tgcttcatag
     1441 cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga aatatcgttt
     1501 gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga acttgcttct
     1561 tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata gtgatccatc
     1621 acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat tgccataccg
     1681 acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc atctgtatga
     1741 tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt caaatcatcg
     1801 attgtcactt gatagccagc taaatgaagc aagctataca acattgctga cccatgtcct
     1861 gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg atttactttc
     1921 aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa tccaggatgt
     1981 cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt aacgccaagt
     2041 tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt ttacctttat
     2101 ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc agtatttttt
     2161 actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct tttaattttt
     2221 ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata tgatgacgca
     2281 taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct tgttcttcgg
     2341 cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc tctatttttt
     2401 caggtgacag catgctcagc cctcctttta ctctccttca tattatataa aaactgaaaa
     2461 aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc gctttttata
     2521 tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca aactgaatca
     2581 agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg atacccgccg
     2641 actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt tcacggtcat
     2701 cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc tcgtgcaatc
     2761 gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat tccagtactt
     2821 ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga cttctttcca
     2881 atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat tcgtggagat
     2941 agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa gcaagaaact
     3001 gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac ttgcaaacga
     3061 ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct cgaaccaatc
     3121 atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag tcatatctat
     3181 taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg gtctcaaact
     3241 cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt tacaaaccga
     3301 acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag aggtgagaat
     3361 tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta ggtattatta
     3421 ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt actttgtggt
     3481 tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt catgaagatc
     3541 atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg actgctaatt
     3601 attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt cacatccttt
     3661 ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat tgcggaaaaa
     3721 gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa atagcgaccc
     3781 tcaaaag
//
LOCUS       DAYYHX010000218         3741 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000218 DAYYHX010000000
VERSION     DAYYHX010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.220"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..115)
                     /locus_tag="ACHA6Y_002567"
     CDS             complement(<1..115)
                     /locus_tag="ACHA6Y_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8988628.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDR"
     gene            246..698
                     /locus_tag="ACHA6Y_002568"
     CDS             246..698
                     /locus_tag="ACHA6Y_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988629.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            712..1401
                     /locus_tag="ACHA6Y_002569"
     CDS             712..1401
                     /locus_tag="ACHA6Y_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988630.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1526..>3741
                     /locus_tag="ACHA6Y_002570"
     CDS             1526..>3741
                     /locus_tag="ACHA6Y_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM8988631.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISF"
BASE COUNT         1261 a          506 c          770 g         1204 t
ORIGIN      
        1 cacgatcata taagatttct tgaacttcac gatagctaag gttataacga agatagtagc
       61 ccacggctac aataatcaca tcctgctgaa attgctttcc tttaaaatga ttcatcgtta
      121 ttcctcctgc tatctttttc tattattcta tcttatttga tagtagattt aaaactttgc
      181 aacagaacca attatcttat cgtagatata tattataaat ttaataagaa agcgaggtga
      241 gaatcgtgaa aaaaacgata ctaagtattt tttcagtatt tacaatatta attgggggct
      301 attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag
      361 ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag
      421 aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa
      481 ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca
      541 aatccgaatt agttagttat aaacaaaatc acagacagtt tattgatgtt aatactgatt
      601 ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa
      661 agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag
      721 atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct
      781 gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt
      841 gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa
      901 attgtatcta tggataatcc agatataatt caaggttcat ataattcaga aactgatcct
      961 gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt
     1021 gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac
     1081 cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg
     1141 cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa
     1201 tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt
     1261 tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg
     1321 cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat
     1381 tatgttgttc aaaatattta ataaaacagc acaaagagat ttctttgtgc tgttttatta
     1441 tttttagttg ttgacaaaca ctaaaatata aaatataatc aacacataaa cgaacgtatg
     1501 ttcggcaaga aagcagggga tttttatgga tgagatggtt ttaagtactc agaaatggct
     1561 aaacaagaaa tacagtaatg ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg
     1621 gtggcctaca atctatggac ttattgaagg actacaagta gaacttggta ttactaatct
     1681 agtagctaat tttggaccta ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg
     1741 gggcaaaaat ctacctaaaa atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg
     1801 gattaatcca ggtggcttcg atggggtata tactccatat ttagatactg ctgtaaaaga
     1861 gttgcagact gatgcagggt ttaatggaag cgctgtaact ggagttttgg atgcgaaatg
     1921 ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt cccggcggag atagtaaaat
     1981 tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa tacacgggaa taatgccttg
     2041 tgatggtatt tatcaaagaa attcaaatca agcattaatt tttgcattac aagctgaact
     2101 aggatattct cccagtgaag caacagggtc ttatggtaat gggacaacta gtaaaactta
     2161 tcctgtgtct gaaggtaact cgggaaacta tgtaagaata attcaatatg gattatatgt
     2221 taatggttat tttgaagacg gtacattcga tggtattttc actaaatata tgggtttaga
     2281 agtactggca ttccgaaaat ttatggtatt gcctgaatat actgaaatag cagatgttgt
     2341 tgtaataaaa ggactattgt ctagcgctgg agatattaga agatctgcag atggcgcaga
     2401 tacttctacg caattaacta gatctcaaat ccaaacattg gttgataatg atataaaaat
     2461 agttggtaga tatcttactg gtactgttgg aattggaaaa gatgaacgaa ataagtatct
     2521 aactacagag gaattgaata atattttttc taaaaattta tctgtgtttc ctatctatca
     2581 agatggaggt gcagcattag cttattttac ttatgatcgt ggcttatcag atggtaagaa
     2641 ggctatagat gcagcaaaaa atctaaatat accattaaca acagttattt attttgctgt
     2701 tgatctagac atgttagggg aagaaattct tgcatatgct ggagaatatt ttaagggggt
     2761 atctgctgta atgtcatata gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg
     2821 ttctcaaata atcaataacg ggtatgcttc gtttgcattt gtttcagata tgtcgactgg
     2881 ttatagtggg aatttaggtt ttcctatgcc tagacaatgg tcttttgatc aatttgttga
     2941 atttaccata ggaagtggca acggagctgt tggtatcgat ttaatcgcta catcgggtag
     3001 agatagcgga tttaatgagt tatcaaatga tactaataat gataattaca ttgccaaata
     3061 caatcagaag gttataaatc agatggtacg tgcgtatcaa tatctatcac aagctggtct
     3121 tgataatcct tggaaccctc atttgacatt ttatagatat gtaaattatt ctggtttgtc
     3181 atgggacatc atttcttctc ctgtaacaga gcatgataga aaaatttatg atgaatatag
     3241 gaataaatta actaatagcg agggcctgta taattatttt atagatccta atactggttc
     3301 aataataggt ttgcctcact taattgtgac gttacaatca cagatgtttc tagccgacac
     3361 aatcaatgat agtgtttctg actttgcagg ttggttagga gatttaatga cttgctgggg
     3421 tgaagttaag aaattaggaa tacaaatgga gcaaggtgta tttgcattag taggtacggc
     3481 tgcaggtgga acatttagtc tagaagattt ataccaagat gttgacgcgg taaatgttta
     3541 taccttagca aaaagaaatt ctgattcaac agctttatcg gctttaaaaa cgtactattg
     3601 tgataagggt gctattgata gatttaatca atttgttaag aacagatttg gttcttcaga
     3661 taaaatcgct tcagtaacaa atagtttatt agaaaatgac gaaacagttg atatcttagg
     3721 tgtagggata atttcatttt t
//
LOCUS       DAYYHX010000219         3709 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000219 DAYYHX010000000
VERSION     DAYYHX010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.221"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..1964
                     /locus_tag="ACHA6Y_002571"
     CDS             51..1964
                     /locus_tag="ACHA6Y_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8988632.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            1969..2970
                     /locus_tag="ACHA6Y_002572"
     CDS             1969..2970
                     /locus_tag="ACHA6Y_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8988633.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(3172..3378)
                     /locus_tag="ACHA6Y_002573"
     CDS             complement(3172..3378)
                     /locus_tag="ACHA6Y_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM8988634.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         1397 a          578 c          757 g          977 t
ORIGIN      
        1 ataaaaaatg tttgcgttta catataattc tcctagaaac ataaaaagga gtgaatagct
       61 tgagaacaaa acaagtatta aagggagcgt tggtcatctt atttgctgta agtacaagcg
      121 tcccgcaatg gacagtatat ggagagagca tttcttccga atcggaatct cgggaggtag
      181 aaaccacaga aactactgat gctggaaatg aaacgaaatc gagtacttca caaacagaaa
      241 caaatactga aacaacggtt gagacatctg ttactagcac agatcaagaa acgaccgcta
      301 ccacagataa ccaacaaaca gaagaagcaa cagctacaaa aatgtcaaac gacgaatcct
      361 cttctgctaa agtaagtgac aaagagaatg ggacagaaaa tgatgatatc ggtaaaggtg
      421 aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa acagatgaca gaaatactta
      481 atggaccaac agaagacatc aagtcaaaat taatagagtt attagtcact gagcttgctg
      541 ttaatgatta tggtgtagat aaagagatta tcaaacatct gattgctact tatctattgt
      601 acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat gcttggaggt acagatgatg
      661 ggacaacgcc tacagaaaac acaggcttct atccaaatac aaaacaactt catcaaactg
      721 tactaaacaa acaagatggc aaaatgatgg atgtatatgc atattatgtc gatcaaggtt
      781 cagataaaac agtcatgatc catggcggat ttcgagggaa ttggaataac gggatcgtta
      841 cagaagaata caatgatttt tataaggccg ggtataattt attatttgtt gattccagag
      901 cgactggtaa tagcggaggc gattatgtga cctatggaca atatgagtca gatgacgtcc
      961 tttactggat aaatcaagaa gttcgagagc ggccatctca aaagatttta ctttatggtg
     1021 gttcgatggg agcagctacg atgatgtctg tattagcgaa agatattcct gtcaatgtca
     1081 aaggaattat tgaaaattgc ggatttgcat caattgatga acaactacgt tttacgtaca
     1141 gccagacagt agcccccgca ctaccggatg ccataaaaaa tcagttagac atcattgggg
     1201 atcaagagca tgaagattta tttatgggcc ttttgaaaca atactatttt gatcaggaga
     1261 tgcatttgga tacaaaagct gctctaccta cgattggaat gtcagattca ttgcctaaat
     1321 tgattatcca tggaacagca gatgatgtag tacctgtgtc taacgcgcaa aaattatatg
     1381 agttatcagg agggtataaa gatcttttat tagtagaagg agcagggcat ggaaaggcac
     1441 aggaagttga ccatgctgcc tacacgaaac atgtaacgga ctttttgaaa gtagtgttcc
     1501 atgatcagat aaacgtaaag tatgtggatg aaaacaataa aagtctactc aaagaccagg
     1561 acgaaatcca gctttacgga gcttatggag aaaattatat gacagagcaa aagacattcg
     1621 aaggatacga attagcaaat gttgaaggac cgactgaagg gatttttaat gagacgatac
     1681 caactattat tttctattac aaaaaaatcc cagtagctcc accaaaaaag caagatccga
     1741 cagagaatgc agacaataag ggaaagaatg aacctcgaga ggatcaatca agtgatgata
     1801 cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac agatgcaaaa aaaagaatgt
     1861 tgccgaaaac tggagaaaag aaacattcgc ttctcattta ttcaggtgga tttttatctg
     1921 ttagcacgat tatcacatgg atttggcgaa gaaagagagg gtaaatgaat gaaatggttc
     1981 aagcgaatag tgattgcagt accagttatc atcgtagtta ttttagtcac tgtatttttg
     2041 attaatcaaa tcacaccaaa gccgattagc ttgcttgtta gaaaacaatt tgacacaagt
     2101 gaaaaagaac aagtatatgc ccgaccagat gatttccagc aaaaaatcga acaaatcgat
     2161 ataaaaaaag aaatatccta tccttcaaag tacaaaaata ataatatgga tatctacaca
     2221 ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga tgcatggagg cgcatatgta
     2281 ggaggggata aaaatgattg tcgtgattat ttggaatatc tatgttcaga cacgcagcaa
     2341 atcatcgtaa atatagatta tgaacgatca ccggaagcaa aacatcctac accagttatc
     2401 caattgaacg aagccattca agcagctaaa aaagagataa aagatcaagc agattggtcg
     2461 aatatttcga ttggtggaaa ttcggcaggt gcacaaatta gtggagaata tttgttagca
     2521 cttcaaaatg aagaaattaa aaaagctgat catcttgatc cgactttgga gaacaagcag
     2581 attacgaagt ttatttcttt gtctggtctg ttggatcctt ctgcacttac tcaagtaagt
     2641 gataaaatct ctagctttct ttatgaaaaa tgtggatggg cttactttga taataaggac
     2701 tttgaacaat cagagaaggt caaaagttta gccttggtca gacatgcgga tcgttggacg
     2761 caaaacacat tcttaacgga tggaaataca aatacgttta cgaaacaaat ggaagaagta
     2821 acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag tagattatgc ggagaaaaat
     2881 gctgttttga accatgaata tcagtttgat ttcagcaata aacaagcgca agaaacatat
     2941 caaaagttag taatgttttt caaggagtaa agtcttatga tgcaaaagag gttgtaatct
     3001 gtcacttagc tccaagtcac aaggagcaat ttcaataaac aagcttttca tggtttatga
     3061 atattgtaac ttgtgactga ggagttagag atggcaaggg gcacctttta ttcgaaattt
     3121 gggaatatca cacgattatt gtcacgatct cttttacgta aattttataa gtcagatttg
     3181 ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct gcggaggcaa gaagggacaa
     3241 aaagccgagt ggtaagcaaa agaatgctgg ttccactaat attccgttgt tattgatata
     3301 aggctgggaa caaagatgaa tagcaaataa aatcaatgca ctagcgaaaa aaagcaaaga
     3361 aatatggata gctttcataa gcagcacctc cttttttata attgtatgat aaatagatta
     3421 agaatctata aatgtgacaa gtttgttagg atgtgaataa aatgatcatc tagcgaactt
     3481 gaaacaaaga tggctaatga taagcaatta atgattcagc aatcagatag gtacaaagtt
     3541 taccatcata cgtctagacc aataatagaa aatatgggaa tataatagaa aagatttagg
     3601 atacccccaa aagaatttcc ccaaaaattc gatgtatcct aaactttttt attttacata
     3661 acaatcatat caactgtgaa ataaaattac aaatgataac ctaaaatga
//
LOCUS       DAYYHX010000220         3678 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000220 DAYYHX010000000
VERSION     DAYYHX010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.222"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..1342
                     /gene="tig"
                     /locus_tag="ACHA6Y_002574"
     CDS             53..1342
                     /gene="tig"
                     /locus_tag="ACHA6Y_002574"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM8988635.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            1589..2839
                     /gene="clpX"
                     /locus_tag="ACHA6Y_002575"
     CDS             1589..2839
                     /gene="clpX"
                     /locus_tag="ACHA6Y_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM8988636.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            2922..3509
                     /gene="yihA"
                     /locus_tag="ACHA6Y_002576"
     CDS             2922..3509
                     /gene="yihA"
                     /locus_tag="ACHA6Y_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM8988637.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         1298 a          615 c          786 g          979 t
ORIGIN      
        1 catgaaacgt ttttgatgta taaaatgaat ttctaaactc ggagggaatt aaatgactgc
       61 aacatttgaa aaaaaaggca cgaatgatgg tgttttaaaa tttacgattg cgcaagacga
      121 aatccaaaaa ggattaacaa ctgcgtttaa taaagtgaaa aaaagcttga acgtacctgg
      181 attccgtaaa gggaaagttt ctcgtcaagt attcaaccgt atgtacggtg aagaagcttt
      241 atatgaagat gcattgaatg ctgtattacc agaagcttat gaagctgctg tcaaagaagc
      301 tggcatcgat cctgtttctc aaccaaaaat cgatgttgaa agcatggaaa aaggtcaaga
      361 ctgggtaatc actgctgaag taacagtgaa accagaagta aaattaggac aatataaaga
      421 tctagttgtt gaaaaacaag accgtgaagt aacagatgct gacgtggatg cacgtatcaa
      481 acgtgaacaa gaagcacaag ctgaattagt tatcaaagaa gatgctgcag ctgaaaatgg
      541 cgatacagta gtaatcgact tcgaaggctt tgtagatggc gaagcgtttg atggcggaaa
      601 aggcgaaaat tactcacttg aattaggttc aggctcattc attccaggtt ttgaagatca
      661 attagttggt cataaatcag gtgaaagtct agatgtaaat gtaactttcc cagaagatta
      721 tcaagcagaa gatcttgctg gaaaagaagc agtcttcaaa gtgacaatcc atgaagtaaa
      781 agcaaaagaa gtgcctgaat tagatgacga attcgcaaaa gatgttgatg acgaagtatc
      841 aacattagac gaattgaaag aaaaatatcg caaagaatta gtagaaacaa aagaaaaagc
      901 agcggacgat gcaaaagatg aagcagcgat ccgtatggct gttgaaaacg cagaaatcgt
      961 tgaattgcca cacgtgatgg ttcatgatga agtacatcgt tcaatggacg aattcttgaa
     1021 caacatgcaa cgtcaaggta tctctcctga aatgtactat caattaactg gttctacaga
     1081 agaagacttg cataaacaat tcgaaggtga agcagaaatg cgcacaagaa caaatcttgt
     1141 gatcgaagca attgcagctg ctgaaaatct tcaagcaaca gaagaagaaa tcaatgcaga
     1201 aatccaagaa ttatcagaaa catacaacat gccaatcgaa caaatcaaac gcgtgttgac
     1261 agaagatatg ctggaacatg atgtgacaat gaaaaaagca atcgaattga tcacaggtac
     1321 agcgacagaa gcgaaagaat agaaatcaag taataaatca tttctgaaga agctacttct
     1381 agaaaattgt ttattcgaaa taagggactg tgacacgatt ttgtcacagt tccttatttt
     1441 ttgttaagag taaaaaatgc aaaggtatag gtcagttgat cctttgttaa acactttttc
     1501 tttttactaa actatgttat gatagtaaaa gactgtaaag tctttgatag tttcttgcat
     1561 actttttgca agttaagggg tgaagaccat gtatgacaat ccaagtagca atgagaccgt
     1621 acgctgttct ttttgcggga aaacccaaga agaagtgaaa aagatcgtag ctggtccagg
     1681 ggtctacatc tgtaatgaat gtatcgacct atgtaaagaa atcattgatg aagaatttta
     1741 tgaagaagct gtacgcgaat tcactgaagt ccctaaacca ctagaaattc ttgaagtatt
     1801 gaataactac gtgattggac aagatcgcgc aaaacgctcg ttagctgttg ccgtatacaa
     1861 tcactataaa cgtgtcaatc agcaagcaca agaaaacaca gatgatgttg aactgcaaaa
     1921 aagcaatatc tgcttgatcg gacctacagg gtctggtaaa acatttttag cacaaacatt
     1981 agcaaaaaca ttgaatgtac cttttgcaat cgcagatgcg acaagtttga cagaagctgg
     2041 ttatgtgggg gaagatgtag aaaacatttt gcttaaattg cttcaatctg ctgattacaa
     2101 tgtggaactt gcagaaaaag ggatcatcta tattgatgag atcgataaaa ttgcgcgtaa
     2161 aagcgaaaat gtatctatta ctcgtgacgt ttccggcgaa ggtgtccaac aagccttgct
     2221 gaaaattcta gaaggaactg ttgcaagtgt gccacctcag ggcggacgta aacatccaca
     2281 tcaagaattt attcagattg atacgacgaa catattgttc atcgtaggtg gtgcatttga
     2341 tggcatcgaa acgattgtga agaatcgtat gggcgaaaag acgattggat ttggtacaca
     2401 aaaccaaaaa ctttctgaag acgaaagtgt catgcagcat atcattcctg aagatttact
     2461 aaaattcgga ttgatccctg agtttatcgg acgattacct gttatggcag ctttagagaa
     2521 attgacaaca gatgatctgg tacggatctt gactgagccg aaaaatgcgc tagtcaaaca
     2581 atatcaaaaa cttctttctc tagataatac cgaacttgaa ttcgaacctg aagcgttgag
     2641 agccattgcg aaaaaagcaa tcgaacgcaa taccggtgct cgaggacttc gttccatcat
     2701 tgaagaaatc atgatggatg tgatgtttga tatcccttca aatgaaaaca tcgaaaaagt
     2761 gattatcaca aaagaagcag ctgaattgtc tggtgagcca acagtaatct ataaagattc
     2821 agagaaaaaa gcgggttgat gaaaagaggc tgccgtaaaa gtcattttgg cttttgcgac
     2881 agccgcttgc tttattcatc accttgttaa ggagagagac catgaaagtc catcaagctg
     2941 aaattatcat aagtgcagtg gcaccaaaac aatatccaga tacacaattg ccagaaatag
     3001 ctctggcagg acgatcaaat gtagggaaat cttcttttat caacacattg attgatcgaa
     3061 agaatttagc ccgtacttcc ggtaagccag gaaagaccca gacattgaat ttttatttga
     3121 tcgagaacgc attacatttt gtcgatgttc cgggatatgg gtatgcaaaa gtatccaaaa
     3181 ctgagcgagc aaaatgggga aagatgatcg agacatattt aacgactaga gaccagctac
     3241 gtgcggtcgt ttctttagta gatttgcgtc ataagccttc tcaagaagat atccagatgt
     3301 atcaattttt gaagtattat gatatccctg taattgttgt tgccactaag gcagataaga
     3361 tccctcgtgg taaatggaac aagcatgaga gcgaaatcaa aaaagcgttg gattttgatc
     3421 gcaatgacac gtttattctc ttttcttccg ttacaaaaca gggaaaagaa gaagcgtggg
     3481 cagcaatcga acaagcaatt gaatcataaa aagaataaaa aagacttacc agtcaaactc
     3541 tttcataaaa gtttggctgg taagtctttt tttgagctta acttactttt gtttcttgtc
     3601 ttttttcttt tcattttgtg gctgtggttt tagaaatcga tcgatctctt cttttggtgc
     3661 aactgttttt tccggatc
//
LOCUS       DAYYHX010000221         3677 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000221 DAYYHX010000000
VERSION     DAYYHX010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.223"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..444
                     /locus_tag="ACHA6Y_002577"
     CDS             <1..444
                     /locus_tag="ACHA6Y_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFM8988638.1"
                     /translation="KEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQREMRK
                     VEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEGLVGKS
                     MTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            444..1643
                     /locus_tag="ACHA6Y_002578"
     CDS             444..1643
                     /locus_tag="ACHA6Y_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8988639.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            1659..2303
                     /locus_tag="ACHA6Y_002579"
     CDS             1659..2303
                     /locus_tag="ACHA6Y_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988640.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            2314..3120
                     /locus_tag="ACHA6Y_002580"
     CDS             2314..3120
                     /locus_tag="ACHA6Y_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988641.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            3142..3372
                     /locus_tag="ACHA6Y_002581"
     CDS             3142..3372
                     /locus_tag="ACHA6Y_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988642.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
BASE COUNT         1360 a          574 c          765 g          978 t
ORIGIN      
        1 aaagaaaaac aaatggaaga acaagacttc aaacgcttct tggcgatgat tgatgagtgc
       61 cataatgcga ttaatccaga ttttattgaa acggtaaatt atgtcacaaa ctttcaacga
      121 gaaatgcgta aagtagaagc aatgactgtt ttagctacac aatccccaca agaaatggtg
      181 ccagaaaata tgtcttctga taacttctcg aaactgaaga aagtctttga attttcttct
      241 acgaagatct ttatgcgtat ggatgaatct attttacgtc atatagaagg attagtgggt
      301 aaatcaatga cgaactcaga attagaatcc attcctcaac aaaatcaagg agacgctgtg
      361 atcaattttg gctcaaaaga aaccattcgt gttcatttta gtccgaacaa acgtcaatta
      421 aaattgtttg atggaggaaa ataatggaaa accaaaatga aagtttaatc aaacagtatg
      481 ttaagcgtcg tgcaaagcgg cgcttatttt tgtgggtgtt tggtacttct gcaggtttga
      541 ttactatatt gatcactgtg tttgtgaccc tttttctaat acttgcggca ggttctattg
      601 ataattctga ttcaagtagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
      661 ccatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgctg
      721 taggtgcagc agtgaagtat aaattattac caagtgtgat cctttcgcaa tacggttatg
      781 aatcagcgtt tggtacgtcg gcgtccgcaa gaaatgatct caattatttt gggattacgt
      841 ggtttgatgg gtgtctgttt cctaaaggaa ctgcgcgtgg aattggtggc attgaaggtg
      901 gctggtacat gaagtttccc aactcaaaag ctgccttctc ctattacggc tttatggtag
      961 ccacacaatc aaattttaat gcttgtgtcg gaaataaaag ccctggcgca agcttattga
     1021 tcttaggacg aggagggtat gccgccgcag gaattactga agacagtgcc tattataaaa
     1081 attgtatgtc tattattaat acaaataaat tgatggagta cgatgagttt gcgattaaac
     1141 actggagaga aggcggcgga actggtggta cgattacagg aagctggggc aatccattcc
     1201 caggaagtag cttagataaa aattcgttca gtggaggaca actttttggg aaaaatccag
     1261 gaggagaatt ccgacctaac gggttccatg atggactaga ctttggttca gtcgatcatc
     1321 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
     1381 tttcaggttt gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
     1441 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
     1501 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
     1561 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
     1621 ttttgaatag cagcaaaaaa tagatcagga gaaaaaatat ggaaaataaa accttaaaaa
     1681 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
     1741 ttaaagaaga acaagagaca aatacctata aaattgaaga agtggctaaa aacgcgtctg
     1801 ataagctcaa aaaaaattct tctgaggcac aggcgaagat cgacaagtta aatcaaacca
     1861 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
     1921 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
     1981 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
     2041 tcgacaaatt aagtcctgca gacgatgatc accaacatga cgactacgga agttactctt
     2101 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
     2161 ttgtcgtatt tgttgattat acaatcggca atcctcaatt taaagatgtc aaaccacaaa
     2221 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcacag
     2281 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agcaagacaa
     2341 cgttttaaaa aacgaaaaag ttatcttaga tggctgggga tctttatgat tctcggcttt
     2401 ttgattcttg ctagtccgat ttacacacat aaaaaggtag acataccaga tgaagtaagc
     2461 acgaacgcat ttttaaacac gaaggaactg acgatggttt caaaagaaaa aggagcgaac
     2521 cagcaattag tttttgaatt tgcgatcgac gatgaagatc agagcttatt aaaagaacta
     2581 gctaatctta attatcgtgt agaagtaaag acagctaaag gggattatca agcaataaaa
     2641 gccaaagtga tcaaggtgtc tgatgattat tttgtagtga agttagcaca tgtaccagaa
     2701 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
     2761 caagaaccac aagatctgat ttattacgtt cacgaagata aggttaaaga tcaagtgaaa
     2821 gatgaagatt atgaacaaca tgcgatcaat tacaaagtga aaggatacaa aaaagaacaa
     2881 aaagcagcac aaaaacaaat ggaatccttc caagcaacca ttgatttgaa tgaagaatta
     2941 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
     3001 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaaat gaaagatcaa
     3061 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
     3121 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
     3181 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga
     3241 gaagtgaatt tatctaatac aacaatatct gagttgaaga atggtcgaaa gaatttagat
     3301 actttaaaat tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct
     3361 gataacaaat aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga
     3421 atcagctata tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc
     3481 taacgcaaac atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca
     3541 accaaacagg aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca
     3601 tatgacaaaa tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac
     3661 acaaataacc ttaattt
//
LOCUS       DAYYHX010000222         3677 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000222 DAYYHX010000000
VERSION     DAYYHX010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.224"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..408)
                     /locus_tag="ACHA6Y_002582"
     CDS             complement(82..408)
                     /locus_tag="ACHA6Y_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988643.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(454..816)
                     /locus_tag="ACHA6Y_002583"
     CDS             complement(454..816)
                     /locus_tag="ACHA6Y_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM8988644.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(853..1067)
                     /locus_tag="ACHA6Y_002584"
                     /pseudo
     CDS             complement(853..1067)
                     /locus_tag="ACHA6Y_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1195..1530
                     /locus_tag="ACHA6Y_002585"
     CDS             1195..1530
                     /locus_tag="ACHA6Y_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8988645.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(1606..3603)
                     /locus_tag="ACHA6Y_002586"
     CDS             complement(1606..3603)
                     /locus_tag="ACHA6Y_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988646.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
BASE COUNT         1146 a          731 c          478 g         1322 t
ORIGIN      
        1 ttataggaaa ctataaaaac tccctagtga atttacacct ttgggagttc ctgtttggac
       61 ggtatgcata atacatttaa atcaatttaa cattaacacc tcagcatatg gtatctccac
      121 atatatacgg tctttctttg gatagtactc aatatttatg ttgatagcac ttccattgtc
      181 taatatagct ttagcccggt atgtgttatc tccattttca tgagtaatcg tcacatcatc
      241 acatgatcga tttaagttgt actgttcttg taaaatctgt gttaccaatg gtttcgatga
      301 cttctctaat ccagatgtac ctgtcatttt tgaaaaaatg atacctataa tagccaagac
      361 aacgataact ataatgataa acttcttgtt catatttact ttattcaact ttttatctcc
      421 tatctccaaa ataatttgct cttatccctc ttatcagtac attgacatcc ctaaaagtac
      481 accaaagatt gctccagcta cccctgctat catcaaaatc gtacccattt gttcatcttt
      541 atctcgatat aagtagccca taataacagc aacagcacca aatacaacag gaataaatgc
      601 taacgctact accatagaaa tccaaccaat aatcaaatac agcttccctg tctcttgtcc
      661 atgtacgatt tgttccttca tttcattaat tgtctgctca gtacttttct ttactgcgtc
      721 tgctccagtt gtctcatctg tttcttgtag tgctccgcaa aactggcaat acttactttc
      781 atctgttatt tctttgccac attttctaca atacatgtct tctcctccta aaaacattca
      841 attgtttatt agttaactgt ttactttatt aaatacatca gaaaacagct cattcattgg
      901 tacacttaat atctcagaaa gcatcttcat ttgatagggt ttaggaatcg ttctccctgt
      961 ttgccacgaa cttacacatt gctgtgtaac attcattttt ttgctaactc cgtttgagaa
     1021 agtccttttt cttttctgta tcggctgaga ttacttactt ttttcatgat tctcccctcc
     1081 tgttttatta tatacaacaa tatgatgtat ttcaactcat aaaatataaa aaaacttcaa
     1141 ataccattac aaatacaaca acttgttgta ctatactgag atgggggtgt attaatgaaa
     1201 cagaaacaat atgcaaaggt cattagagaa aagcgaaaag ctctcgggtg gacacaaaaa
     1261 gaattagctg aaaaaatctt ctctacacaa caggcagtag caagatggga aaattctgta
     1321 acagagccta acctcgatag cttaaccgct ctttctagag ccctaggaac ccctgtaagc
     1381 cattttctag acaatgttgt cgtagattat gaggaagagt ttttggctct ctatcgttct
     1441 cttagcactg aagatgcagt tcgaacaatt gactatatga agctattaaa aaggcaagaa
     1501 aatgaacgca atcagctatt gaaagattga aaaataaata acccaccgaa ctttaacggt
     1561 gggtgtattg agatgaaata agattaagag ctatggataa cctgtctagt ttaatacaat
     1621 tgggtctttt ctcttcctta ctctccgtcc atcatctctt gttacacttg taaattgata
     1681 aagaggaact aattcaactc ctgataatat caattctttt attacttcat ctatccctgc
     1741 tccttttgct aggtagacta aaaaactact acttgctttc acattgtata gtctcttaat
     1801 atccttagaa gctcgttcaa gagtgaatcc ttgtttaaca aagtagcctc tgttttcttt
     1861 taaagcattt tccacgctgt tattcccaac attacgtaga cgttcacaaa cagtgttagt
     1921 aagttctttc tctgtcatta gatatgttcc ttgccacgat aagccctgta aaacttcatg
     1981 tgcttcatct aaaatcaatt gtttttcctt gctaagttct gatcgggaat tttgaggaaa
     2041 tactctttta gcctcttttt cgttaaaaca tatcttaacc cattcataag aaaaatattg
     2101 atatgtaaaa tgaaattttt ccagaagtgg aaggatatct tcattcaatt tttgaatcac
     2161 agaagttgca ttaaaaacgt ctaatcgata gacattgctt ctcttatgat taaagtttcc
     2221 taacgctttc tcttccttta acctatttag gtattcgagc atcttcaaag gaacatcttc
     2281 atcatctaat ttttttatca atcctataaa agtcataagt gatagtaact tattacattt
     2341 atctatgtca taagttttac catagtaaaa actcattttt ttagctaatt gttcccccga
     2401 caaaatacaa gtcacacttt ccttactatc atcaaaaaaa ttaacatcag aaagcaagac
     2461 atcatagaca cgtcgcattt cctctagata attccttatt gtcctgaaaa gtttgtcttt
     2521 cattgattca agagtgtcaa aaagtacttt tgattcctct ttcaattttc gaaaatgtgt
     2581 aggatacaag tctaggttct ctaatagcca attaaatgtt tctacccgac tgtctttctt
     2641 taaaattagc atcacaactc caataaaatt ttcactgata tcacaattac tggaatgaca
     2701 ataaaaaatg gaatgcccag ttttactggt aaaaatacta gcagagggat tatgatcgtt
     2761 gtgaaacaga catcttaagt ttttacttga cttaagtaat ttattcatat caatggttaa
     2821 caatctttca taaatctcat taaaagacat gtcggaggaa tcaaacaggc agtctttaaa
     2881 ccactgtttc atttcttctt tgtcgtcatt ttttatcaac ttaacaaaat cggtttcatt
     2941 gactagttca tactctctcc ctgaatcact ttgacaattt ttagaacatg tcatggtaaa
     3001 atcaatcgga agctcagtaa tatctaacat gttgtcttca ttaattatta tcactgcatc
     3061 tgacttatat cctccaaaaa acattctatt cgaagcattt acattgtcat cacacccggg
     3121 gatgttcttt ttcaagtatt cgtaaacagc aaccatttta ccatagtcag taattgcttc
     3181 ttttaataga aagacaatct tgtatttttc tagattttca gagtaccgaa tacttttttg
     3241 tatcgcaaac gcagaattat gaactaaatt gatagataaa accttttcta tgcttaagta
     3301 tctactatca tctttactca aatctactac cttccccttc tcatctttaa cagtgttatc
     3361 gacatccaga ataattactc tggtttccac aaaactctcc ttgtcattgc ctttattctt
     3421 tgtcttgctt ggaataaatg attgtgatga atttagtatc tctacaaact catctcttgt
     3481 aacttctcta tagttatcta aacacctatc tcttacatca gttttttctt tttttgtaac
     3541 tggcttctct aataattttt ctttatctac ataaattgga atattaatca ttgatgtttc
     3601 catttgtgtt tcctccttat aaaattctgt taaagactaa aacatacaga taataaaaac
     3661 aaactcttta ttactat
//
LOCUS       DAYYHX010000223         3641 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000223 DAYYHX010000000
VERSION     DAYYHX010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.225"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..754
                     /locus_tag="ACHA6Y_002587"
     CDS             92..754
                     /locus_tag="ACHA6Y_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8988647.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLATIITERCPLEFVNEFREKTTIKEA
                     IQ"
     gene            751..1665
                     /gene="pfkB"
                     /locus_tag="ACHA6Y_002588"
     CDS             751..1665
                     /gene="pfkB"
                     /locus_tag="ACHA6Y_002588"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8988648.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1683..3614
                     /locus_tag="ACHA6Y_002589"
     CDS             1683..3614
                     /locus_tag="ACHA6Y_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8988649.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
BASE COUNT         1172 a          644 c          836 g          989 t
ORIGIN      
        1 actgatttat aatttttatg aacgtttttg actgatttat attgctagta gaaaaaaata
       61 tgctattatt tgtattgagg tgatcgtgaa aatgcttaca gaggaacgca gacaaaaaat
      121 cctagacttg cttgaacagc agaaaatcgt gaaatcacaa gatcttgtaa accttttgaa
      181 tgcttctgaa tcaacgataa gacgtgattt acaagagctg gagaatagtg ggtttctaga
      241 aagagttcat ggcggagcta agaaagagca attattgaat tttgagcaga atatgagtga
      301 aaaatcactc aaaaacgttc acgaaaaaca gcaaatcgcc aaatcggcag ctgaaaccat
      361 taatgacgaa gatgtcattt atttagacgc tggctctact acgttagaaa tgattccttt
      421 tctgcaagga aaacagatca cagtagtgac aaattccgtt caacatgcgg caaatttagt
      481 tgatttggag cttgctacca ttattttagg tggaacgata aagctggcaa ctaatgctgt
      541 tttaggatct aatgcgatag aacaactaaa aaattttcat tttaataaag catttatggg
      601 aatgaacggt gcccatcttg aacaaggatt cacgactccg gatccagaag aggcagctgt
      661 taaacgttta gccacgatca taaccgagcg ttgtccatta gaattcgtta atgaattccg
      721 tgaaaagaca acaattaagg aggcaataca atgatttaca cagtaacact taatccatca
      781 attgattata tcgtacgcgt agatgggtta aaactaggcg atttgaaccg aatgaaggaa
      841 gacttcaagt tacctggagg aaaagggatc aatgtttccc gtattttgaa acggatcgat
      901 tctcaatcta cagcactagg gtttttagga gggttcactg gcgaatttat ttcgaagtgg
      961 cttgaaaaag aacaaatcca gtctttgttc acaaccgtaa aagaagatac aagaatcaat
     1021 atcaaactaa aatcagacac tgaaactgag atcaatggct taggcccatc tatttcagat
     1081 gaagaaatcg atgagttgaa gcaagcatta ggaacagtag gacaaggaga tatcgtcgta
     1141 ttgtcaggaa gcgcgccagc tagcctgcga aaaggatttt atcaagaact gattgatatc
     1201 attcgagaaa aaggtgcaga tttcgtgatt gatacaacag gtgatgacct aaaaaatgca
     1261 cttgttaaga aaccgttgtt gatcaaacca aacaatcacg aattagctga gctttacaac
     1321 acaaccttcc aatctgtaga agaaatcatt ccgtatggga aaaaattact agctgaaggg
     1381 gcacagaatg tgctgatctc aatggctgga gacggtgctt tgctcttcac ggaacaagga
     1441 acttataaaa cgaatgtatt agttcgccca ttgaaaaatt cagtaggtgc aggggattca
     1501 atgattgctg gctttattgg ggcttacagc cgtacgaaag atccaatcga agcctttaaa
     1561 tggggagttg cttgcggaag cgcaacagct ttttctgatg acttggcagc tcgcgaattg
     1621 attgatgagt tgctgccaga agttgaaatc acaaaaattg attagtttca aggaggataa
     1681 tcatgaatat caaggattta atggtcaaag aagcgatgat catggatttg caagcgactg
     1741 ataaaaaagg cgcaatcgat gaaatggtcc aaaaaatgtt tgatgccggc cggatttccg
     1801 atatcgaaac atataaagaa ggaattctag cccgcgaagc acaaacttct acaggtcttg
     1861 gtgatggaat cgccatgcct cacgcgaaaa acagtgcagt taaagaagca acagttttat
     1921 tcgcaaaaag ccaaaaagga gtagattatg aagccttaga cggacagcca acttatctgt
     1981 ttttcatgat cgcagctcca gaaggcgcaa acgacacaca tttgcaagca ttagcagcac
     2041 tatctcgatt gctgattgat gcagatttcg taggaaaatt aaaagaagca aatacaccag
     2101 aagaagtcca agacttgttc caaacagctg aagagcaaaa agaagcagaa gaaaaagcag
     2161 aacaagaaac acaagctgca gaagcttctg ctcatccaga aagaaaattc gttgttgccg
     2221 ttacagcttg tccaacaggg atcgctcaca cttacatggc agaagatgct ttgaagaaaa
     2281 aagcgaaaga aatgggtgtg gagatcaaag tcgaaacgaa cggttcagaa ggaatcaaaa
     2341 accgattgac tgctgaagac atcgcccgtg cagaaggtgt aatcgttgca gcagacaaaa
     2401 aagtagagat gaatcgcttt gacggaaaag aactgatcaa tcgtcctgtc agtgatggta
     2461 tccgtaaacc agaagagttg atcaatctag cccttagtgg tactgcacca gtattccatg
     2521 gtgatggaaa agaaagcagt tcagaagaag gaaatgcgga tggtactatc ggacaaagaa
     2581 tttacaaaga tttgatgaac ggcgtatcac acatgttacc tttcgttatt ggtggaggaa
     2641 tcgctattgc gctttctttc atgatcgacc aatttattgg cgtgccacat gatcaattat
     2701 caaatctagg gaattacaat caagctgcta gctggttcaa ccaaatcgga caagctgcat
     2761 ttggtttcat gctgccggta ttagcaggat ttattgcttc aagtatcggt gaccgccctg
     2821 gtttgatcgt tggatttgct gctggtgcat tagctaatac aggcggtgca ggattcttag
     2881 gtgcactgat tggtggattc ttagctggtt atgtcatcgt atttttacgt aaattattca
     2941 agaacttacc aaaatcattg gatggaatta aaacgatcct cttttatcca gtgtttggct
     3001 tactgattac tggtttcttg atgttgctgg tcaatgtccc tatgaaagca atcaatgatg
     3061 cattgaacgg tttcttgaca agtatgagtg gttcgaatgc tgctttactt ggtgcattat
     3121 tagctggaat gatggcggca gatttaggcg gtccaatcaa caaagccgct tatgtgtttg
     3181 gtacagcaac tttagctacg actgttgcaa ctggcggaag tgtcgtgatg gcttctgtta
     3241 tggctggagg aatggtacct ccattggcaa ttttcgttgc tacacgtttg tttaagaata
     3301 aattcagcaa aacagatcaa gatgctggtt tgacgaacat cgtgatggga ctatcgtttg
     3361 tcacggaagg atcgattcca tttgcagcag ctgacccgat tcgtgccatt ccaagtttta
     3421 ttattggttc tgctttaact ggcggattag tcggagcttt cggaatcaaa ttactggctc
     3481 ctcacggcgg gattttcgtt gtattcttac tgagtcaccc ggtcatgtat ttggtgttta
     3541 ttgctatcgg agcagtcgta tctggtgtga tttatggttt attgagaaaa gctcctgcag
     3601 tagcagaagc atagaaataa aaaagaagag gctgggtttt c
//
LOCUS       DAYYHX010000224         3615 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000224 DAYYHX010000000
VERSION     DAYYHX010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.226"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..568)
                     /locus_tag="ACHA6Y_002590"
     CDS             complement(<1..568)
                     /locus_tag="ACHA6Y_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8988650.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLK"
     gene            729..1418
                     /locus_tag="ACHA6Y_002591"
     CDS             729..1418
                     /locus_tag="ACHA6Y_002591"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8988651.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            1432..2934
                     /locus_tag="ACHA6Y_002592"
     CDS             1432..2934
                     /locus_tag="ACHA6Y_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8988652.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2947..3423
                     /locus_tag="ACHA6Y_002593"
     CDS             2947..3423
                     /locus_tag="ACHA6Y_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8988653.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT         1183 a          513 c          660 g         1259 t
ORIGIN      
        1 ttttcaatga atcataagta tctttcgttt tcccagataa agaaaaaatg attactaaat
       61 cattaactgt taataaagaa gcgacttggg cttgaaaatg agggtctagt acagcttttg
      121 cttgaactcc tacgcgaaga aacatatttt ctaaatcaag actagtattt ccactagatc
      181 ccactccaaa tatatatatg ttttttgatt gagtgatcat tttaatggct tcattcaatt
      241 tttcttcgtc taaaagccta atagtggaat agagagcttc tgttaaacta ttagcaattt
      301 catcatagta ctttacacta gtcggatgtt cttttttttg actaaagtct tcctttgcaa
      361 tttcaatttt taaatcagaa aaaccagaaa aaccaatttt ttgacaaaag cgaataatag
      421 tagcatcgcc aacttttgtg tttttcttaa tatcactcat cgtactataa ataatagttt
      481 tcccagaatt taaaataaaa tcagcgactt ttttttcaga cttcgttaaa aatggataat
      541 tttctttaat taaatagata taactcatac aaactcctct acaatttcaa cttttctcca
      601 ttatactcca aaataataaa aaaaactcct aaatactcca tttttatctt tacttttatg
      661 aaagcggatg ctatattgtt tttggagttg ttatccaaaa ataataaaaa attggagtgt
      721 ttaggataat ggataaaaag aggtttttag atcttatttc aaaaggtttg attgtttcct
      781 gtcaagcatt acctggtgaa ccattttaca aagaggaaga aagtttaatg ccttattttg
      841 ctttggctgc tcaaagggct ggagcagttg gtatacgtgc aaattctgtt cgagatatta
      901 ttgaaattag aaagtgtgta tcattaccaa tgattggaat tattaaaaga gattatccac
      961 cagaatctcc attcattact gccactatga gagaagtaga tgaattagta gaaaccggtg
     1021 ttgaggtaat cgctcttgat tgcactctta gaaatcgaca tgatggacta tctataaatt
     1081 cttttattac taaaataaaa gaaaaatatc ctcatcagtt atttatggca gatatttcta
     1141 catttgaaga aggcaaaaat gcatataaag caggaataga ttttgtagga actactttaa
     1201 gtggatatac tgaggaaagt aaaaaacaag atgggccaga tatagatttg attaaaaaat
     1261 tggtagaaga agggatttgt gtaattgctg agggaaggat tcatactcca gaacaagcaa
     1321 aagagataaa aaaaatagga gttgcaggta ttgttgtggg tggtgctatt actcgaccac
     1381 aggaaattgc atcaagattt gttaaagcat taagttaggg ggagaaataa aatgtttaaa
     1441 aaattttcac aattaggacg cgctttcatg ttaccaattg ctatattacc agtagccgga
     1501 ttactattgg gactgggagg agcgttaaca aatgagagtg ctataaatgc ttatccattt
     1561 ttagatcaac cgtggttgca tacaatttta agtattatga gttatgctgg aaatgcagtt
     1621 tttgcaaacc tagcactaat ttttgcaatt ggtattgctg taggacttgc aaatggagat
     1681 aaaggaacag ctggtttagc tggtggagta gcttatctag tatatactgc aactattagt
     1741 gggtttcttg aacttttttc agcaaaagat gcaacactag atacaggtgt agtgggatca
     1801 attgttgttg gtggtactgt tgcattttta cacaatcgtt atagaaaaat tgaattacca
     1861 caattcttag gtttttttgg aggatcaaga tttattccga ttgtttctag tttagctgca
     1921 atcattattg gttctttctt ttatttaatt tggccaccaa ttcaaaatgg tttagtagtt
     1981 gctggtgaac aaattgccca aatgggtagt ttaggaactt ttttatatgg ttttttattg
     2041 cgtttaacag gtgctgttgg actacaccac accatttacc ctttgttctg gtatacatct
     2101 ttaggtggta ccgaaactgt agctggatca acaattgctg gagcccaaaa tattttcttt
     2161 gcccaactag ctgatccaaa ccacactggt ctgtttactt atggtactag attttttgca
     2221 ggaagatttg caacaatgat gtttggatta ccagcagcat gttatgcaat gtatcgtgca
     2281 attcctaaga aaaatcgtaa aaagaatggt gggctttatt ttagcggggc ccttacatca
     2341 tttttaactg gaattactga accagttgaa tatatgtttt tatttgttgc tccttggctt
     2401 tatgtgattc atgcattctt agatggatta tctttttatt ttgctgatat cttaaatatt
     2461 cgtgtaggga attccttttc aggtggtttg atcgactttt tattattcgg tgtacttcaa
     2521 gggaatgaca aaactaattg gttaaaatta attccttttg gtattgcatg ggcatttata
     2581 tatttctttg tttttacatt ctttatcaaa aaatttaagg tagctattcc tggaatggaa
     2641 gaagaatcag aagttcattc tgaaaaagta ataaacaaaa atacatcatt acatgaagag
     2701 tctcttatca ttattgaagc tcttggtgga aaagaaaata ttgagtctgt tgaagcttgt
     2761 gcaactcgtt tgcgtgttgc tgttacgaat ggggagatta ttcaaaaaca aacaataaaa
     2821 gacactggtg caactgctgt cttcgaagtg aagggtggca tccaagctgt ttttggaggg
     2881 aaagctgact tattaagtca agaaatcaat caaatattag gaaaagataa ttaggaggtt
     2941 tttaatatgt ttgaaatttt caaaaagaaa aaacaagaaa ctttttatgc accttgttcc
     3001 ggaaaaatca tttccatcac agaagtaaat gatcctgtct tttcacaaaa attactaggt
     3061 gatggttttg ccgtcatacc tgatggttca aaaatttatg ctccattggc tggtcgagta
     3121 acaaatatat ttcctactaa acatgctatt aatataaaaa gcaactcagg cattgattat
     3181 ttgattcata ttggaattga tacagttgaa ttacaaggag agcctttttc gatttgtgtt
     3241 caagaaaatg atgaggttac tcctgaaacc tcgttggttg atgtggattt tgacaaaata
     3301 ataaaatctg gtaaagatac ttctgttata gttgtttttc cggagaaaaa tcagttcaat
     3361 gatttaataa tagataatgg tcgaattgct catgggaatt catgtggaaa aatcactttt
     3421 taaaaaagtg tatataaaaa gctataaaat aagctaaata ggatatttgt caaatgaagt
     3481 tggtgaacag aaagaactgt tttctttctg ttcatttttt tattttatta gtaatgattc
     3541 cccatgccaa atcatgatcc gtaatttaaa gttataaaag cttcgaaatc cattggcatt
     3601 tcgttggaat acctt
//
LOCUS       DAYYHX010000225         3595 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000225 DAYYHX010000000
VERSION     DAYYHX010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.227"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            163..2223
                     /locus_tag="ACHA6Y_002594"
     CDS             163..2223
                     /locus_tag="ACHA6Y_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM8988654.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2306..2875
                     /locus_tag="ACHA6Y_002595"
     CDS             2306..2875
                     /locus_tag="ACHA6Y_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8988655.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3050..>3595)
                     /locus_tag="ACHA6Y_002596"
     CDS             complement(3050..>3595)
                     /locus_tag="ACHA6Y_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988656.1"
                     /translation="LGELSDFYGYFSMWDTFLHAFSGFLAGGVGFSLVYLLNKNTKTM
                     NLTPLLLAVVSFCFSMTVGVMWEFVEFSADKLLQFDMQKDTIIQQINSVELSKDGNTV
                     YHVPDITRTIIESQNADGKTDKTIIQNGYLDVGIMDTMKDLFVNFIGAVVFSVFGYLY
                     SRNDYQKFTFIKNFIPKKIKR"
BASE COUNT         1283 a          652 c          660 g         1000 t
ORIGIN      
        1 aaaagtacat aagttattat tccgattttc ttgaaacaat aaaaaatcaa aaaaaatact
       61 ctcctaatag tccatcttct tttgatttta tttatataat gaaatcatct tatgaaaatt
      121 actacttcat gtttagacaa gagtagggag gactaagaat aaatgtgttc aaaaagacgt
      181 tcaaaacaac taaataaaca cgcttgtcgt ttagataagg aacggtcgtg ttctgaaaaa
      241 cactacgtta agtttatgat gcgcacgatg ctgatcagtt tatctgtagg tgtctatatg
      301 catgtacccg ttcaggcaac gacacaaacc atcaaggtcg tacgtacctc tgtttctgag
      361 accactgatt cccctatcac tattactcat actttcactg atgcaaatgg aaaacagcta
      421 cttcctgcat tgacttccta taataaagtc agttatcctc caacgataaa ccactactgg
      481 ttaattccta acaaaacatt atggacatcg gaaaccggaa gcagtgtgcc gctttctatc
      541 ttttccgata tgactgatcc tacaaaaggt gatcagttaa aacagatcac tgatattttg
      601 aatcatgctt tgacagatat aaaggacggt ggaagcgcac aagtttctta tgtatatgaa
      661 aaagattatt cacagtttga aagaaagaaa atcgatgttc tggcagggac aaaagtttcg
      721 atatctgatc tggtagaaag gctgaagaac gcagatggaa cacaaggaga tatgtccaaa
      781 gtaatttcaa acaatgggaa tatcgtggat acgactaata caggaacatc tactatttcc
      841 ttgtcctatt ttgatccaat tgctttaaaa acaatgactg catctgctgt gatccatgtt
      901 cgtgctgatc aaacagctat caaaggaaaa gatcttcact taagaacagg agagagttac
      961 gcccctaaag atcttgttga atatgtaaca aaaagcgatg gaacttcagg aaataaagaa
     1021 gaaatcaaaa tgaacgggac agtagatact acaactccgg gagaatatcc cgttgtatta
     1081 acttatatag actcgaaaac atcaaatgtg gcagaaacaa cagctgtagt ttatgtagaa
     1141 gcatctgctg cttcatcgac tgtcatcact cattttatag atagtcaaac aaataaagaa
     1201 cttcaagcag aaacacatat gagtagtgta aatcgtattc ctgatttgcg cctacctgct
     1261 gaattttctg atcgagtgcc aaatatagat ttatcgactt atgaacaaaa tgggaaagtc
     1321 acttctattt ctgattggat gcaagcaaac ggattagaca gaaaaagtca ctggtcggag
     1381 atccttgaag tacttcactc agaactgatg aataatagga agcaatcagg aggggaaatt
     1441 acctttactt atgtttatat accagacaat cgttcattag aaggtcatga tcttattgtt
     1501 catccaaacg aaaaaataaa caaagaagag ttgatccaat cagcaacaaa ttccttaggt
     1561 aaacccgtac cgttaaagga tgtatcggtt acaatcaatg aagtaccatt ggaggatggt
     1621 tatttgacca aacaaacagg tgattttacg attatgtata gttatcatga cccgtattca
     1681 ctccaagaac tccatgctca gtcactatta catgttttac cattaaacaa acagatagat
     1741 caaaaagaac aaaaaagaca agaagaaagc ttgtcagaaa aaaaacaaca aatggctcat
     1801 gcagtttctc caacagggaa agtaaaacaa acaagaagcc tagagttagc aagaatatct
     1861 agaaaaatct cagaatctaa taaaacgata aggcaagaag ctcatttggc cccacaagtt
     1921 tctggattgg aaaatgaaat tttagagcct aaaataaaac aacaatcaga aaaagaattt
     1981 gttgctaaac agtcggaaga tcagaaggaa tcagatgacc agaaggaaac gaagaaaaaa
     2041 cgcttgtcaa ctattgattc caccgatgag catctgcagc cgcaagtacc acttcctagt
     2101 ccgggaacac ccgcacctgg tggaggtatt ccttcttctg ctttaggaga tttcatgcga
     2161 ggtatggcag gaacatgggt atacgctaat cgaaatgaat ttgacgcaga tgactggttc
     2221 tgatttatat ctctatctgt tatagtcaaa taagtagctg agtcatatgt tatttggcat
     2281 aatggagacg aggagggata gaaaaatgtt agataaaacg ttaccttacg cagaaatctg
     2341 gatgacaaga ccacttcaac aaaatgttcc aatcacgcct ttagcggaag gttttgaatt
     2401 tcgattttat caaaaagaag atgaatctgc ttgggcggag attgagactt ccgttgggga
     2461 atttcaaacg acagatgaag cacttgagta ttttcaacaa aactttgctc cttatcctga
     2521 agaacttgaa agaaggatgt tttttgtaga gacaaaaaaa ggagaaaaag tagcaacctg
     2581 taccgcttgg cgaaaaaaat caagagagca agtgtatccg gtttttcatt ggttagcagt
     2641 gaaaccagat taccaaggaa aagggctcgc aaaagcattg actgccagag tcttgcaaga
     2701 atttcctacc ctccatacgg acggaccaat ctatcttcac acacagacat ggagccatca
     2761 ggcgattgct ctttataaaa aaatgggttt tacttttatt gctgaaaatc tagacggcac
     2821 acctaatcca gattatgaaa aagttatgca ggttttaagt aaattagata aataacgaaa
     2881 tggtgaaata agtcctcagt attgggataa aaaacgaaat agtcattcct catatcttac
     2941 ttttttgagt taatactatc ggataactaa tggcatgcca tacattcaaa aaagaagggg
     3001 cagtgacaaa agtattgtca ctgccccttc tcttgttttt tagcatgttt tatcgtttga
     3061 tttttttagg aataaaattc ttgatgaacg taaatttttg gtaatcgttt cgactataga
     3121 ggtaaccaaa tacactgaaa acaactgctc caataaaatt aacaaataaa tccttcatag
     3181 tatccatgat tcccacgtct aagtaaccat tttggatgat tgttttatct gttttaccat
     3241 ctgcattttg actttcaatg attgtccgag tgatatctgg aacatgatag acagtgtttc
     3301 cgtctttact taactccaca ctattgattt gctggatgat cgtatctttt tgcatatcga
     3361 attgcagtaa tttatctgct gaaaattcca caaactccca cataacacca actgtcatcg
     3421 aaaaacagaa ggaaacaact gctagtaata aaggagtcaa gttcatggtt tttgtatttt
     3481 tattgagcaa ataaacaagg gaaaagccga cacccccagc taaaaatccg ctgaatgcat
     3541 gtagaaaagt atcccacatt gaaaaatagc cataaaaatc acttaactct cctaa
//
LOCUS       DAYYHX010000226         3497 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000226 DAYYHX010000000
VERSION     DAYYHX010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.228"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..1787
                     /gene="yfcC"
                     /locus_tag="ACHA6Y_002597"
     CDS             48..1787
                     /gene="yfcC"
                     /locus_tag="ACHA6Y_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM8988657.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1944..3128
                     /gene="iadA"
                     /locus_tag="ACHA6Y_002598"
     CDS             1944..3128
                     /gene="iadA"
                     /locus_tag="ACHA6Y_002598"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM8988658.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3239..3442
                     /locus_tag="ACHA6Y_002599"
     CDS             3239..3442
                     /locus_tag="ACHA6Y_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8988659.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         1112 a          590 c          817 g          978 t
ORIGIN      
        1 attttttatt catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa
       61 attcttcagg aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca
      121 ttatttttgg tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt
      181 tcagcacgca agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt
      241 tacgtcaaga ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat
      301 tagaagaact gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat
      361 tggaaaaagt cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct
      421 ccttgacaga tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa
      481 aattgcataa aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa
      541 attttgtttt tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg
      601 cattgatcct tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg
      661 caggtatcta tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat
      721 tattctttgc tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat
      781 tctcaatggt gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa
      841 ccgtgactta tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg
      901 tagcggtggc tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga
      961 ttatgtgggt agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa
     1021 tccgtaaaaa accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata
     1081 tcaaaaagac atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag
     1141 agatgttagt tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc
     1201 cagaaatcgc ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt
     1261 tcaaattaga tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg
     1321 atttagctgg tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag
     1381 gatctgatgc atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt
     1441 tgaccggtgt tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca
     1501 acttggttgt tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac
     1561 tggcagattt agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag
     1621 gttttatgga cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac
     1681 ggatcgaatg ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt
     1741 taggaactat tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg
     1801 taaataaaaa agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa
     1861 aatcaaagga gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat
     1921 acttaaagaa agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag
     1981 attacttagg aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac
     2041 agctagaagg cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac
     2101 taaccccggg tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt
     2161 atcaaaatcg aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag
     2221 ttgtcggttg tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag
     2281 cgaaaggtct ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc
     2341 cggtaaaaac agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg
     2401 gaatcggaga aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg
     2461 ctcgtgcagt agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca
     2521 atgtccactt aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa
     2581 cagaaattcc gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg
     2641 aagcttgcgt ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc
     2701 ctgatttttg ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga
     2761 tcgaggaaga tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc
     2821 cttattttga tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc
     2881 tagtagggat caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag
     2941 ctataaccag taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg
     3001 gtgctgatgc agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg
     3061 ctaaaggaga aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa
     3121 gtttttaatc aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat
     3181 ctgttgttga tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat
     3241 ggcagattta aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga
     3301 agcatacggc aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact
     3361 aggtgctgac atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc
     3421 tcgaaaagac gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa
     3481 tccgaaccat atctgcg
//
LOCUS       DAYYHX010000227         3469 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000227 DAYYHX010000000
VERSION     DAYYHX010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.229"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACHA6Y_002600"
     CDS             complement(<1..2232)
                     /locus_tag="ACHA6Y_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8988660.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACHA6Y_002601"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACHA6Y_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          973 a          768 c          549 g         1179 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaact
//
LOCUS       DAYYHX010000228         3405 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000228 DAYYHX010000000
VERSION     DAYYHX010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.230"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            145..1845
                     /locus_tag="ACHA6Y_002602"
     CDS             145..1845
                     /locus_tag="ACHA6Y_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM8988661.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            1815..2033
                     /locus_tag="ACHA6Y_002603"
     CDS             1815..2033
                     /locus_tag="ACHA6Y_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM8988662.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2053..2943
                     /locus_tag="ACHA6Y_002604"
     CDS             2053..2943
                     /locus_tag="ACHA6Y_002604"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM8988663.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            2961..3245
                     /locus_tag="ACHA6Y_002605"
     CDS             2961..3245
                     /locus_tag="ACHA6Y_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988664.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         1201 a          552 c          721 g          931 t
ORIGIN      
        1 tttcaattaa tttttgtgat ttttgtattt ttttatttaa aggaggtttt atgaattgtt
       61 ttcttatgga atctacaaag tcgaatacga gactcatgac ctttttatga cgtactggta
      121 tacttgaaga aaaaggagag cgcgatggag ataaaaaaag tttacttttc acagaaaatg
      181 tcgctgtatt cgcctatcca gcaagcaaaa caaccccttc cattttttca aagtcatcaa
      241 gaaagtagag acgctttttg gcgttctgca aaaaagcaat gtgcttttaa agcagtatcg
      301 aaaaaatata ccgtctattt tactattgct aaaagtagag gagatgttat ttttaataat
      361 ctgttgattg aaggtgaaac ttttgaatgg aaaaaatttt ttcggtatat ggaagcttgc
      421 gctcgttttt ttattaaaga aaactgttgt tttttatttc aatctcctct atcagaagaa
      481 tggttgcgta tttttcataa aaacggctat caaggaacca cacaattaaa taaaaagctg
      541 gtctatcata cagcacttgt tttaggtgga ggaggagcgc acggtgctta tcagattggt
      601 gtgtggcagg cgctgaagga acatgatatc aactttgaga ttatcacagg tacctcggta
      661 ggtgcgttga acggcgcatt gattttacaa ggagatatga aaaaagctgt gaatttatgg
      721 aaaaagctgt cgacaagaca agtgcttgcg ttaccagaaa tggctgcagt tgaagacctt
      781 cgtgaacgct tctatcgaga aagacgccaa atgacgaaaa cagcactaat tgaaggcggg
      841 gtttcttctg ctccattgga aaagatggtc aaagacaatt tggatttgtc cttactcaaa
      901 cataatccca aaatacgttt atatacggtg tctactaaac tgccagaact agcggaagta
      961 gtgactgata tccaacagac aaaaacagaa gaaataccag attggatact cgcctctgct
     1021 tcattttatc ccgctatggc ttatcgaaag atcggaaaaa ataaatatgc agacggtggg
     1081 tatcgcaaca aaatacctat tgatattgcg attaacaaag gagcaacaga ggcatttgtc
     1141 gtagatgtgc aagggccagg cccagcgaaa aaaattcgta tgccagacat ttttatccat
     1201 tggaaatgtc aaacattgtg gacactaggc agctttctgc tttttgatag ccaaaggaac
     1261 caattaaatt tacaattagg ctatctggaa atgaaaaaac gattaggcta ttactatggg
     1321 aactggtata catttcattc tgttaaacct gcaagaacat actggcgagg atttttgtct
     1381 tacttaacaa aagaaattca gttagaatta tcttttttca aaagcataaa attttggcaa
     1441 aaactgcgta atttatataa gaacagggtc gtaccagaga catgcggatt ggcgatgcta
     1501 gaacttttag caaaaaaaca ttttttactg cccaatgaaa tctatcaagt agacatgatg
     1561 attcaaatga tctgccaaca agatataaaa agtatgccgg atgatttatt ggcacaaatt
     1621 ggtcaattaa gtactgaaga atggcgaagg tatcgaaaat accagcaaaa aatacaaaat
     1681 gagcgcacga aaactgttta ttttgattat ctgctacaag gaaaacgcaa ggatcgtttg
     1741 cagcatgaat tgcttaagca accagttgac acattactga tcctatattt atattatctc
     1801 aaggaggaac aaccatggca caagaatttt catatgaaat cgtagaagaa atcgctatat
     1861 tgtcagaaaa caacaagggg tggcgtaaag aactgaacct agtcagctgg aatggtcgcc
     1921 cgccaaaatt cgatcttcga gattgggcgc cagaccatga aaaaatgggg aaaggcctga
     1981 cattgacgaa cgaagagttt gaacaactgc aaaaagcaat agaaaatatg taatgaggaa
     2041 ggaccctgtc gaatgaaaag tatgactggt tttggcaaag ccatccgcga aacaaaagcg
     2101 tatcagttag atattgaaat aaaaagtgtc aaccagcgtt ttctagatgt tcaaatacga
     2161 agtcctaaaa tcctgaactt tgttgaaaac gattttcgtc agcttattaa acagcagtta
     2221 agccgcggtc gaatcgaagt atttgtgaat ctagcttata ttggcaaaaa cgaaaaacag
     2281 atcgtcatca actgggaatt gattgattca cttatcactc aattgacaga cggaattcaa
     2341 caacgatacg gtagtcaaaa tcaagtgtca attagtcgga tggcggaacg cttcgcgctg
     2401 gacgaaaact tcgtaacggt agaagaaaaa ccggaagaga gcatggaaga attggcacag
     2461 ttagctctag atacattgaa aagcgcactt gattctattg aggcgagtcg agaaaaagaa
     2521 ggtcaagctt tagctttagt gttgaaaaag aatactgaag aattcaaaga agtactaaaa
     2581 aaactcgata gttttgtaga aatctatgaa aaggaatttc aaataaggta tcaaaaaaag
     2641 ctggaggaat ttcttggagc gaaagtcgat gagcaacggt tgttgacgga gctagccatc
     2701 ttgttggaac gcggagatat tcacgaggaa ctggatcgtc tggcgatcca tgtacaaaaa
     2761 ctagatgaac ttatcgaagc acaatgtcct gtaggtcggg aattagattt cctgatccaa
     2821 gaaatgaatc gggaaatcaa cacgatcggt tccaaatcaa atgcaatcga aattaaaaat
     2881 caagtcgtcc agtctaagac gatcttagaa aaaatcagag aacaagtaca aaatattgaa
     2941 tgacgaaaga ggtagagata atgaaatatt atgacgtaac gttccacgaa ttaagtggaa
     3001 aaactgtggt taaacgggat atcccttctg aaaaaaacgg ctttgacgta tggaaagatg
     3061 cctgtgcaga ctataatgag aacgaactgt ttattttgat caacgacggt gcttacgtga
     3121 cgatgaatcg gaaatttatt gttcgaattg acacagaaga ggtagaagat ccgacagaaa
     3181 aagcacgttc tcgaaaagat gagatcatgg gtgtagtgaa tactttgtcc aatatgggct
     3241 tttagcaagg atgctgaaca aagaaataca ataaataatc agaattggat ttcttcgctt
     3301 agcttctttg ctctaggaat acatttaaag gttgtgacaa aattcgtgtc acaatctttt
     3361 tttattttga ataagtgatg ttcaaaaacc attttctcgg tatta
//
LOCUS       DAYYHX010000229         3350 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000229 DAYYHX010000000
VERSION     DAYYHX010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.231"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..870
                     /locus_tag="ACHA6Y_002606"
     CDS             70..870
                     /locus_tag="ACHA6Y_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFM8988665.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            886..1764
                     /locus_tag="ACHA6Y_002607"
     CDS             886..1764
                     /locus_tag="ACHA6Y_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFM8988666.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            1777..2757
                     /locus_tag="ACHA6Y_002608"
     CDS             1777..2757
                     /locus_tag="ACHA6Y_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8988667.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            3028..3120
                     /locus_tag="ACHA6Y_002609"
     CDS             3028..3120
                     /locus_tag="ACHA6Y_002609"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8988668.1"
                     /translation="MPRRSFDEIFKITAVKLVTEEGFSVKEVSL"
BASE COUNT         1161 a          546 c          701 g          942 t
ORIGIN      
        1 gacagataat caaaaagaat ttatagttgt taccgtagta gctactaaaa ataaagtgag
       61 gattaatcaa tggaaacgat tattcaaatc atgaaaaaat atgatttttc agagatggag
      121 acaagagtgt ataccacact attagaaaaa ggtaatctta caggatatga agtaagtaaa
      181 atctcaggtg tgccacgctc gaaagtatat aatattttgg aaaaactatt aaaaaagaat
      241 ttgattgtcg ttaataaatc agaacctaaa ctttatcatg ccatctcggc aaatgagttt
      301 ttagagaaat tagaaaaaag tgttaaaaat gatctttctt ttcttaccaa gaatctaggg
      361 atgatcaaag aaaaagatga agaagaaatg ctgtggaaag ttgatggtat cgagtatgtt
      421 ctggataaag tagagcattt ggtgaaaaac gcaaaagaaa gtttgctcat tcaagtttgg
      481 catgagaatt tcacggattc actactaaag gcattgcagc aagctgaaaa aagagttgat
      541 aagtttgtac tgattttatt cagttctacc catgaatatg atcttccctt agaaaagtat
      601 tatattcatg gttttgaaac agacaaactt gctgactttg gagcaagatg gatcaacatc
      661 gtcgccgatg agcaagaagt cgtgtttggt accattaacg aagaactgca atcaaccgat
      721 gtcacttgga caaaaaacca cgccatggtc aatctagcaa aagaatatgt caaacatgat
      781 gcatatacac taaaagtgat tgcagaatct cctgaagaac ttaaagcaaa atatggtgag
      841 ggctttgagg gaatcagaaa gatttactag taggaggaat tttttatgat tcggaatatt
      901 ttgctgacac taattgtcgc aatcaatcta tattttatta tttactttct aagagacctt
      961 ttcaaaaaca aacaaaattt caaagaagaa ccaggggata tgcgtctgtt gcccttcacg
     1021 tcatttatca tcttctttct ttcaacattt ggcgtttccg actttgctat cggaaccgtc
     1081 ctttatccaa agttgaaatg ggtcagcatg aaaaaactac ccggaacgtt gaatacccaa
     1141 tgtgtagtac cggtagccgt catggcattg tcttacatca ctgccatcaa cgtagggatc
     1201 aagacactag ctgtttgtat tatctgccaa attatcggtg catatttagg accaagattt
     1261 gtcgtgaagt taccagagaa aacgatcaaa ctgtttgtcg gcataggatt ggtcattgct
     1321 gcattgttga tttttgcagg acaaatgaac tggattccat caaacggaac agccagtgaa
     1381 ttgtatggcg gaaaattaat cttggcagga tttttactct ttgtctatgg tgcattgaac
     1441 aatatcggca ttggttctta tgcgttaacg atggtaactg tctacttgct aggcttgaat
     1501 ccagttgcag cctttcctat tatgatgggg gcttgtacct tctcagtacc gatcggcagt
     1561 gttcaattca ttaaatttaa tgaatacagc agaaaaatca cactttttac ttcaaccttc
     1621 ggtgtattgg gggtattggt cgctgtattc ctagtcaaat ctttagatac ctatatcctg
     1681 aaatggatcg tgattttggt cttactatac agtgcatata cgatgttggg cagtcaatta
     1741 aaaaaagtta cagcagctaa ttaaggagcg attcaaatgt tatttcttaa aaaagaagat
     1801 attatcaaaa gtttttcaat gagagaagcc atcgatgcgg acaaaaaagc actgtcattg
     1861 tattcagcag gaaaggcaag tgtgcctctt cgcaccaata tcgatgtgcc aaaaagcaac
     1921 ggacaaagct tatatatgcc tgcttatgta gaaggtggcg aaggcgcgct aggtgtcaaa
     1981 atcgtttccg tgtatcctga aaacatcaag aaaaacttac caagtgtacc agcaacgatg
     2041 atcgtattgg atccagaaac gggtatggta tccgcttgtt tggatggtac ttatttgaca
     2101 caattaagaa caggcgctgt tcaaggagca gcaacagaac ttcttgcaag agaagatgct
     2161 aaaattggcg cgctgattgg aaccggcgga caagcacagt cacaattaga agcaatgttg
     2221 accgttcgta aattagaaga agttcggatt tttgatattg attttgagcg tgccagccaa
     2281 tttgctgaaa aaatgatgca acaattttct gtaacgatgc gcccaacaaa aaccagtcaa
     2341 gaatgcgttg aaggagcaga catcattaca agtgtgacca cttcaaaacg tgcgacattc
     2401 tctgctgagt gggtcaaaaa aggtgcgcac atcaatggag tcggtgctta cacaccggaa
     2461 atgtgtgaaa ttccacgaga aataatcaaa gccgcagatg ttgttatttt tgataccatg
     2521 gacggtgttc taaaagaagc tggggacttc atttcaccgc tgcaagatgg gtatattcaa
     2581 agagacagct atcatgggga actaggccaa ctgatcaatg aagaattgat cggtcggaca
     2641 agtggagaac aaatcacgat cttcaaaaca gttggttcgg ctgtattaga tgtggtagtt
     2701 gcgacagaaa tcgttaagaa agctaaagaa aataatttgg gaaaattgat tttctaatta
     2761 aaaaggctga aattaaaacc tttattattg cgtttaaaaa aagaaaacaa agaagtttgt
     2821 aatcaaatgg aaactgaccc cataatttaa ataaaataaa aaacatctct tgttgaattc
     2881 tagtaatgaa gcgaccctaa aacttagacc aaaaaatcta acttttgggt ggagttgtca
     2941 agaaaaaccg ggcacttagt taaaaaataa gttggacggg ttatactaaa attgacacca
     3001 aattaagaga aagcgaagat ctggattatg ccaagacgtt catttgatga aatatttaaa
     3061 attaccgctg taaaactggt aacggaagaa gggttttctg ttaaagaagt tagtctatag
     3121 ctagaagctc gctctaatag cgtgtattga tgagttcaag aagttgaaaa atatggataa
     3181 cgtacttttc tcggtagagg aagtcatgaa atccattcgc agaaatgaaa ttacacgctt
     3241 agaaaaagag aataaacggt taaaagaata ggtagaactc ctaaaaatta ccgaagtctt
     3301 cctgaaagaa aaggctcttc gtcaaattgt gttggcgaag acaaatttga
//
LOCUS       DAYYHX010000230         3331 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000230 DAYYHX010000000
VERSION     DAYYHX010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.232"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(492..3197)
                     /locus_tag="ACHA6Y_002610"
     CDS             complement(492..3197)
                     /locus_tag="ACHA6Y_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM8988669.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT          870 a          673 c          610 g         1178 t
ORIGIN      
        1 taagaaaata attggaaaaa aacagaaaaa tatcaagcgc tttttcaaac gctttaatac
       61 agcaaaaatc taacattttt ttatgggctt tttctttgaa ttctataata aaaggataga
      121 acttgactta gaaaatcaag ttctatcctt tttgatatct taacttttgg aagagagatg
      181 taaggctgct ttctacccat cattcttact actcgaagca tagcgcagtt gtcacaacct
      241 cttgataaac ggtgtttaaa agcttgcatc tgcggtaata agcccaacca aattaaaaac
      301 ataagaagct gtttttattt ggtcgttctt attacttgat gcagagcagc ttttttacaa
      361 cctcttggta cacggtgttc aatcagctga ccctcggtat tagctcaaaa aaacggcaaa
      421 atatgaggaa ctatttttcc atttttttct ctaatactca ggtcaaaaca ctgatttcac
      481 aacctctttt tttaatgaaa aatatggctc tcatgcgccg cttctttgaa tttcgtcatt
      541 ttttcttcga tgaacggata gattgccgtt cctacgattc caaagacaac aaacaaaccg
      601 atcatgatcc agattgcttt cgtagcattt ggccaataaa ttcctcctgc agattcccgt
      661 aacaagttta ccgcatgagt aaatggcaag aacggattga tcatttggaa gaatttccct
      721 gagacttgga tcggatagtt cccgcctccg ccagagatcg acagtactaa gatgataata
      781 gcgatccctt taccgacatt atcgaataat gcagccaaga catagacgat catcatgaac
      841 gctaaggcga tcaagacagc aaatgacacg ctgtagaccg gattttggac atctactcct
      901 agtaagaaaa tattccctaa tgtaacgatc aagctttgcg caatcgccat tgtcaaaaat
      961 gtcagcatac gagcaacgaa ttgttctcgt ttcgtaaaat tgtttcggtc tttctttcct
     1021 aaatagtatt ctgtcgtagc aacacttgaa agaagaacag cgccgaccca cagacatagt
     1081 gcagtataaa acggggtgct agctgaaccg ttatttgcga ttggataaag cgcattggtt
     1141 tgtagctcaa caggttgtgt aaagaagtca ctttcttttg ttgcgtctag tttcaataat
     1201 ttgatgactt cgcctagatc gacttcttgt tcgccttttc ggatcgcgtc tgctgctttt
     1261 tggataccag ctttgatatt tggccagtca ttgttgatca agtccgcagc agcgttcaca
     1321 gctgtttcga cttctggcat tttttcattt actgttgtca atgtaccggt gatttcattt
     1381 tttagtgctg gccaatcatt ttggataaaa tcagcggcca atcctaattt ttgctgaatg
     1441 atcggtaatt cattgttgta taggtctgcc ccttcattga tgccattgac gatggtatcc
     1501 atatttccat tcagcattgt attggcatca tggatttctt gtccaattgc aggcatttct
     1561 ttttgatatt tttctaaaat tgccactgca ttcgttacag ttgctttcgt tgagctgaga
     1621 agcgaatcaa agtcgatctg gtccgctttg ctcagtgcgc cttgtgcagt cccaatcgta
     1681 tcgatgactt ggtctagtag agaagtgaca gtggaactga tttgacctgt attgatacca
     1741 gcaatgatgc cattgatatt attaacaacg gcatcaattt gatctagcaa ttcatcaata
     1801 gccttcgtgt cttcattttg aacagctgtg ttcaaagcag ataggcggtt ttgcagattg
     1861 cttagcaagg gttttaattg atttagctga tcaataattg gctgtaattt cgtatttcca
     1921 gcacttgttt gcaatttagt caggaactcg accagtttat cgattgcatt ttgttgttga
     1981 gatattccgt caataaagcc gttcaataca ttgctgatgt ttttcttttc atcatcagtc
     2041 agcttaccat cttcaatagc tgttcttaca gtatctatta acgaacctgt cgttgttgcc
     2101 acttgtccaa ttgacttcaa tgtaacatct acactgtttg tgatgcttgg tatcgcatct
     2161 ttcagctgag aggcacctga ttttgtttgg tttgctagat cacttgcctg attttctaaa
     2221 tttttcacat ctggcaagat atcttgtact tgttggatga tcgtcaagcc ttgttttgct
     2281 tcatcgattc cttggttcat tgtctcttgg atcgaagcaa aatcgccgtc gatctctgct
     2341 agttgcttac cggcattttg gatctctggg attttttctt gcaaatcaag gatgattttt
     2401 gcttgatctt tcaactcagg cattttgtca ttcaatgcaa cgactttttc acccatctca
     2461 tctactttag ggatgtaatc cacgaattca tttgctttat tcagtttttc cttgatttca
     2521 ggtagttgac tttgtaattc gaggacttgt tttgtgtagc cgtcgatcgt gtccaaattt
     2581 tcatctgttg ataaaatcat atctttaact ttattgatac ttactaggtt cgtatcgatg
     2641 tcgtaaccta tctcattgaa tactttcaat aaagtcgaac tggctgtttt gataaattca
     2701 tttgtgattt gcgattggat cgaacttgct cctttatcgg tgattttcgg tgcaatcgca
     2761 ttgatttttt catttaccgt atattcgatt tttggtttct tgatatcacc actagtaaaa
     2821 ctcagcagat cttctgagaa atcctttggc aagtaaatac ctgcgtaata ctttccggaa
     2881 cggactccgt cttctaattg ttcttttgaa tcaacgaatt gccatcctaa ttgtttattt
     2941 ttatgaagcg attcgatgac ttgctcccca atggccactt ctttaccttg aaactctgct
     3001 ggcttgtctg cactgtaaac agcaattggc agctctccag tatttccata tggatcccat
     3061 aatgctttga tgttaaacca cgcatatagc gaagggatga tcatgatcgc gatgatcaga
     3121 agtgttgcaa tcgggttttt gaaaatccgt ttccagtcaa gcaaatataa tcgaagcgta
     3181 tttttgattg actgcatagt tctccccctt cttctttttt cagctgaatt actttaccat
     3241 gtatttcata cttttttcaa tcggcttttt tcaaaaaagt cgaatttttg gacatatcac
     3301 ggcaattgtc tcattgtaat ctcttacata t
//
LOCUS       DAYYHX010000231         3321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000231 DAYYHX010000000
VERSION     DAYYHX010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.233"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            178..468
                     /locus_tag="ACHA6Y_002611"
     CDS             178..468
                     /locus_tag="ACHA6Y_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988670.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            481..1506
                     /gene="sppA"
                     /locus_tag="ACHA6Y_002612"
     CDS             481..1506
                     /gene="sppA"
                     /locus_tag="ACHA6Y_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8988671.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            1509..2168
                     /locus_tag="ACHA6Y_002613"
     CDS             1509..2168
                     /locus_tag="ACHA6Y_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8988672.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            2325..3257
                     /locus_tag="ACHA6Y_002614"
     CDS             2325..3257
                     /locus_tag="ACHA6Y_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988673.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
BASE COUNT         1130 a          532 c          734 g          925 t
ORIGIN      
        1 aaaatcataa ccttttgcga atgttcttgt caacaaaaaa agaataaaaa ataaattggt
       61 atatatcaaa ttctttgtca aatgtccttt ttatgtcaaa aaataatggg tttttatcta
      121 tcatcttttt caggagttcg gtacaataga aagtaatcgg atagaggagg agtaaaaatg
      181 gattcaaaaa ttgagatcat gacactggga atgttaaaaa aacaattgtc caaattcgaa
      241 gcatcagctg gtgtatcaga tgatacgaaa atatttttag atactggctg ggatagcatc
      301 caagaaatcg cacctgatgc cttggaagtt gctcaagctc gtgagtttac ggtagaagat
      361 gaattaacaa aagagtcgtt ttccgggtat gcgcgggaag aaaaagcaga gcggttcgac
      421 acttctgagc agtcagaaac tgtgatcgtc ataaaaaact tgtattagaa aggagaaaga
      481 atgaataaaa gacgttgggc agcagttctg attgctgcag gattatttgt ggtttcgtta
      541 gtttcagcta gtttgactaa gcctcaggaa aacgatcaaa ttgagggatt gaacagttgg
      601 ttatatgggg atgaagaact agctccaaat gtgttggaag agggagacag tacgcaaaaa
      661 atcgtaaaat tatcagtaga tggaacgatt gctgcgggag ggtcttcagg tttatttgct
      721 tctgaaggtt acaatcataa aaattttatg gaacaactga aggcgattga agaagatcca
      781 acagtcaaag gtatcttttt agaagtaaat tctcctggtg gcggtgtcta cgaaagtgcg
      841 gaaatcgcta agaaattgga tacgattcgt aaagaacatg atatcccaat gtatgtcagc
      901 atgaaaaaca tggcagctag cggtggctac tatatttctg ctcaagcaga taaaatcttt
      961 gcaacagaag aaacagtgac cggctcaatt ggtgtgatca tgtcaggatt gaattattca
     1021 ggacttctag aaaaactggg tgtggaagat acaacagtta aaagcggagc gttgaaagat
     1081 atgggttcag gaacgcgacc agagacaaaa gaagaagaag cggtgcttca agcatatatc
     1141 gacagcgcat atagtcgttt tgtagcaatc gtcagcgagg gacggaataa atctgaagaa
     1201 gaagtcaaaa aaattgctga tggaaggatt tatgatggtg tccaagcaaa agaagtaggt
     1261 ttagtcgatg aattgggatt tcctgaagat gctttaaaag ctatacgaaa ggaacaacag
     1321 cttgaagatg cggaactagt agaatattct tccacgccat ctgggtttgc tagtacatgg
     1381 ttaggaagta aactagcaga attccaggga ttgaagcctt ctgagactag tcagatctta
     1441 tccgttcttg agaatctagg gacagcggaa tcaccaaaag caatgtatta ttacggaggt
     1501 gagtaagcat gtcagagctt tcaaatgaaa ataaggaaca agaaaaagat aagcaacagc
     1561 agcctgctgt aggacctgac tccaactttt cccaaaaggt actgagctct tttgaaagag
     1621 agcctttgac gcccgaagaa atcaaaaaga aacaacgtaa atggaaaaag tatacggaca
     1681 caagaaatga accgccaaaa gcaaatgatt ttcctaagta tttctatgca ggttttttta
     1741 tccgaatgtt tgccttcttg gtcgatcttt tgtgtatcgg agctattacg cgtttaacac
     1801 ttggtattgc caaaaatcta ggttggctta atttttcaga ttcttatctg agtctctacg
     1861 gattcacagc cttacttatt tatctagcgt attttatttt attgacgaaa ttcaatcatg
     1921 gccaaacgat tgggaaaatg atctttggta ttcgagtcat cagctttgat gaagaagaac
     1981 ttagctggtc aaccgtttta gttagagaag gtgcttgccg gttcattcta aaatttcctt
     2041 tgctgatgtt agggtatctt ccagcagtct ttaatcagaa aaagcaacat ctgggcgatt
     2101 ttttcactaa tacaagtgtt gttacgatca atctgatcaa agcgttcaac caggaagtca
     2161 acagctgatg agcaataaaa agcagtaaca gttgaaagca ccttctaaaa gtcagatcta
     2221 aaatctagct tttagagggt gcttcagtta tttttaattg tcatgctttt tttgttttgc
     2281 tactattgaa catgagctga atatgagcaa gaggaggaca ctgcgtgaag ccgattgatt
     2341 ttccagaaaa atatgagaat ttaatgcgag tggcacaaca agcactagca aaccagcaat
     2401 atcagcaggc aaaagaatta tttcaacgag cttacgagtt gaaagaaagc tttgaggcta
     2461 atagcctgct tgttttttgt ctttacgagt tggacgagaa aaaagaagca ttgaagcaag
     2521 cactcttgca tgaaaaacag tatcttcaaa atgaggagtt tgctgaattt tattttgact
     2581 tgttaatcag tacacaagat tttttatatg ctagaaaatt gatagcaagt acagattttt
     2641 atgaagcttt cgagcagcgg gtcattgaaa aaatccaatt tgcagaagaa ttatccggac
     2701 aaatggagag gcagaagatc aaggcactcc acaataaatc ggaggaactt ccttcgttgg
     2761 aacctgcacg tcagcttgcg tctattgatg agatcgaaca gcttccttat catgagttta
     2821 tccaaacagc gagtaaactt attgtatcgc cagaggtcca tattttagca agggcaaaat
     2881 tactggagac acttaggcaa ttgaacgaat gccatcctgt tttctatttg acgattgaag
     2941 aaaagttagt caaagtcata ccaaaagatc tgccaaagcc gcagcagcag gcaagttacc
     3001 agcaattatg tgtattttca gatcgttacg ggaatgaaga tgcgctgctt agttccgtgt
     3061 taaaagaaga attcacgttg caatctgcaa ttgtttatcc tgtctatgat acatacatcg
     3121 aagacccgaa gcgctggttt caactgactg tagaggcgtg tacaggaaaa acgatgtatg
     3181 aaggtacaga agatgaaaaa caagattttt taaaaaaaag agaaaaaatt ttgcagcaaa
     3241 tgatcttctt ccattagatt ttatcagtga aaaagataaa aatgttatga aaaacaagga
     3301 ttctatccat tcaatatgtt t
//
LOCUS       DAYYHX010000232         3295 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000232 DAYYHX010000000
VERSION     DAYYHX010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.234"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(103..354)
                     /locus_tag="ACHA6Y_002615"
     CDS             complement(103..354)
                     /locus_tag="ACHA6Y_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFM8988674.1"
                     /translation="MEQLALVNLSALKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            complement(368..559)
                     /locus_tag="ACHA6Y_002616"
     CDS             complement(368..559)
                     /locus_tag="ACHA6Y_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988675.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            852..1172
                     /locus_tag="ACHA6Y_002617"
     CDS             852..1172
                     /locus_tag="ACHA6Y_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8988676.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            1190..1612
                     /locus_tag="ACHA6Y_002618"
     CDS             1190..1612
                     /locus_tag="ACHA6Y_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8988677.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            1695..2330
                     /locus_tag="ACHA6Y_002619"
     CDS             1695..2330
                     /locus_tag="ACHA6Y_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM8988678.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            2448..>3295
                     /locus_tag="ACHA6Y_002620"
     CDS             2448..>3295
                     /locus_tag="ACHA6Y_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8988679.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFD"
BASE COUNT         1204 a          529 c          585 g          977 t
ORIGIN      
        1 caagccctat aaaaatccaa accatgtaca caatcgttcc tatcaacgct tgtctatttc
       61 cccaaagccc taaagcgtag atgattagcg gtgcgctgaa tactagtgct ctgttgaatt
      121 tgcccatgtt tttcctcctt ttagatactg atacaaagcg atactactaa acctccactc
      181 tctgccaact ttcgctgctg gaatctttcc agattcggca tctttagtta gcgtgcgtgt
      241 cgtggttttc aaatattccg cagcttgctt tgtatcccac acttcatttg caatctcaga
      301 ttctgccaaa gaagctttga gggctgagag gttaactaaa gctagttgtt ccattgatta
      361 attcctccta tcgaattctg taatcacgga tgatttctaa gatagtttta ttagcttttg
      421 gtccactcca atggccatca ataatttgct gcattcgaac acgtgtgtat ccatatgcag
      481 tagccaaatc ttccatagtt actccgtttt ctcgcataaa cttcttgatt gcagcacggc
      541 cgttatctag atttgacatc tattcactcc ccttacatat agatttgtaa gtcaaaatga
      601 tagaaaaaac gtataaacta ttgactaata gtatacaatc gtatactata taaacatagt
      661 taaataagcc tacaacaaac cctttattat gcaatcggtc gccaaactta atgctataag
      721 gtgtattttt agtttgcttt ttttctatca aattaactta caaacaaata ataatacaaa
      781 cttatactta tgtcaacagt ataatttaca ttttgtgtac ttttatttgt ttaggattgg
      841 agaacattat tatgacactg tttgaaagga taaaatcatt agctagccaa agagataaaa
      901 gcatgaaaga agtcgcttta gaattaggat ttagcgaaaa tcttttctat cgatggaaaa
      961 caacagaacc caaagcaaga gatttgcaaa aggtagctga ctatttcaat gtctctgtag
     1021 actatcttct aggtagagaa gaaaaagaaa cgcctaaaca tgtggattta tcagaagatg
     1081 atactgtatt ttcttttgat ggaaaagaaa tatctaagga gacaatgcgt aaagcgattg
     1141 caattgctaa agctttagag gaaaatgaat agttggagtg atgggttgta tgtatttaaa
     1201 gttgaaagaa atgctgagtg agtataattt aaagttaatc tatatggaaa tggaagaacc
     1261 aggtttttat tatccaaaac caagaatagt atttttgaat gaaaaactac acgaagacag
     1321 ttctgaagct tttcatttag cccatgagct cggtcatttc attgcttcac attttgaata
     1381 ttcagtactg tacgataact ctacaacttt tcattcaaaa ttcgaagctg aagctgatag
     1441 aatcgcaatt atgattttac taaatatctt tattgagaac gaattgactg atgaatctca
     1501 gttcaaatta gaaaatttca tggaattcta ctctatcaat aataagttaa gaacagaatg
     1561 ttttaatgtt tgccagtcat atttcaagaa aaaatactct tatgcacagt aaaaaaagcc
     1621 cgtgctgcaa cacggactca tacctcattt ctgagatcac aaatatatta taacaagaag
     1681 tgagggatat ttaaatggca aaaaaagtta tgggtcaaga tgggaaaatg tataaggtta
     1741 aaaaaccgtt ttataaacgg gtatggtttt gggtattaat agtacttttg ttttttggta
     1801 ttggtggtgc tctaagtggc ggtgaaaaca aagattcagc tactgcaaca tcaacaagta
     1861 ctaataaaga aactacaaaa gaaactacaa caaaagaaga aaagacttat aaaatcggtg
     1921 atgacgttgc tgtagggaaa atggaataca aagtaaactc cgttgaagta gtaaaacaag
     1981 ttggcccatc tgtacttcct actaacgcaa aagacacttt cttagtagtt gacttatctg
     2041 taaaaaatgc aggtgataag gctgtgactg ttgatagctc attcttcaaa ttaaaagcag
     2101 atggaaaaac tttcgaagct gattcggctg catctatgtc ggcaaatcaa gatgaaaatg
     2161 gtaatatcac taactctttc tttatggaaa gcttaaatcc tgatatgcaa caaactggta
     2221 aaatagtttt tgatatctct gaagcccaag caaatgcaca aaataatgta ttgcaagcac
     2281 aaacaggtta ctttggaaca gagacagttt caattgctct tcataactaa ttagtataaa
     2341 aaacacgccc caccgaccaa agcgagcgtg ttctaagaaa aaacaaacct acacaatagg
     2401 cttctttata gtacctattg tatcagagaa agagagctga ttcaattatg tcagaagtaa
     2461 caggatactt agaacaagta gacaatgaaa catatagact aagagctgta ttagggtaca
     2521 aacctgatgg ctcggcaaaa agaaaaagca aaactataaa agcgaatagt aaaagagcgg
     2581 catataaaga attgaacatc tggttagagc aattcgaagg aatgaccgat gattcgttgg
     2641 atttattcaa tataactttt ggtgaatttt atagaaaaat atggctttca gaggccgaaa
     2701 aaaatttaga gccaaagtct tatcataact ataagcgaat gatcgagaat agatttcttg
     2761 ataaatttga ttttatccct ctaatcgata taaaaccata catgataaag aaaattgttg
     2821 ttaatgctca aagaatcaat acaaaagatc ctggaagaaa ctctgataaa cctttatcaa
     2881 gaaatacaaa gcttcgtatg ttatacgccg ttaacaactt atttttgatg gctaaaaatg
     2941 agtacggagc aattaaagaa aatcctgtgg aaaatgtaaa aataccaaaa gaaaaaggcg
     3001 taaaaaagaa tatcgaagaa ccttattcgg aagaagaaat tcatgcaatg ctaaaagcag
     3061 catttgagga aagcatagaa attaaaactt taattgttct agcatttatt acaggagcaa
     3121 gacaaggaga aattgcagct ttagaagaaa aagatattga ctttgacaaa caagaaataa
     3181 gatttcatca gcggatttca gaagtggatg gaaaatcaga tattcggttg ctgccaggtt
     3241 tgaaaaatga tgatgatgaa aaaatagtaa ccggtcctgc ttatctattt gatat
//
LOCUS       DAYYHX010000233         3279 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000233 DAYYHX010000000
VERSION     DAYYHX010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.235"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..525
                     /locus_tag="ACHA6Y_002621"
     CDS             <1..525
                     /locus_tag="ACHA6Y_002621"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8988680.1"
                     /translation="VSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDLVVIRLELN
                     GKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVRSIITKKEN
                     RQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTVMEGGEDAF
                     TNRAKRYGFKQIVE"
     gene            575..964
                     /locus_tag="ACHA6Y_002622"
     CDS             575..964
                     /locus_tag="ACHA6Y_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8988681.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            951..>3279
                     /locus_tag="ACHA6Y_002623"
     CDS             951..>3279
                     /locus_tag="ACHA6Y_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8988682.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1059 a          546 c          707 g          967 t
ORIGIN      
        1 gttagtgggg aaagtagtat tttttcagat aaaaaattaa tcaattctat aagaaaattg
       61 aatgatgcac aagttcttga attacaaacc cttaagccaa atgatttagt agttatccga
      121 cttgaactaa atggaaaaaa agttagttta tcgaatgcta gtgctggtca aaagacatct
      181 gcaattcttt ctatgatttt ggcgtatgga aattcaccac tcatattgga tcagcctgaa
      241 gatgatttgg atagtcaatt aattaataac ttaatcgtaa gaagtatcat tacaaaaaag
      301 gaaaacagac aaattttaat tgtaactcat aatgcaaata tcccagttaa tggtgattca
      361 gaatggttga ttagcatggg agattcaaaa gaaatatcgg ctgatcagtg cgggtcggta
      421 gatgaaccca atatcaagcc aagaatatgt actgtgatgg aaggtggcga ggatgctttt
      481 acaaatagag cgaaacgcta tggttttaaa caaattgtag aataacacta gagggtgaga
      541 catattatta tgtccgccct ttttaggagg aaaaatgaaa aaaataaaaa gctataccag
      601 tatctgggca gttgaaaaag tcatttacgc aatcaatgat tttcagttgc cttttcctgt
      661 gacttttaac caaatggctt ggttcgtttt atctttactg tttgtcatcg tatttgccca
      721 tgtgcctcca ctatctatga tagaggaggt ctttttaaaa tacttaggca taccggtggc
      781 tgtcacttgg tttatgtcgc aaaagacgtt tgatggcaag aaaccactgg gatttttacg
      841 atcctttatc agctatcact tgcgatttaa ggtgactttt gcaggaaaga aagtcaaaga
      901 acagaagaaa cgatgggatg aacccagtac tcttgtaagg agtgtgaact atgtatccga
      961 ttaagtatat tgaaaataat ctcgtcttta accaagaagg cgagtgtttt gcctactatg
     1021 aattagtgcc ttacaattac tcctttttgt cacccgaaca gaaatttcaa gtgcatgata
     1081 actttcgcca actaattgcg caaaatcgtg aaggcaagat ccatgcgtta caaattgcca
     1141 cagaaagtag tattcgcgcc acacaagaac gatcaaaaaa agaaattacc ggtagactca
     1201 aagaagtagc aaagcaacga attgatatac agacagatgc attagtatcc atgattggcg
     1261 atagtcagat tgattatcgc ttttttattg gctttaaact aattgcgaca gatgaagaag
     1321 tcaatctaaa gagtctcaag aaatctttct tttctgggct tcaagaattt gtctatggag
     1381 tgaatcatca tttgatgggc gattttgttt ccttatccaa tgaagaaatt cgctgctatt
     1441 ctaagcttga aaaattaatg gaaagcaaat tggctcgaag atttaaagtc aggcgagtca
     1501 caccgagtga cctgacgtat ttgattgaac atatttatgg cgaaaaaggg ataccgtttg
     1561 aagaatacga atttcagcta ccaaagaaaa agctaaaatc agaaacactg gtgaaacgtt
     1621 atgacttatt acgtccaaat cgttgtttaa ttgaagaaaa accaaggtgc ttacggatgg
     1681 aacatgagaa tcatgagtcg tacgtagcgt atttaacaat taatacaatt gtgggggaga
     1741 tggaatttcc ttcctcagaa cttttctatt accagcaaca acaatttaca ttcccaatcg
     1801 acacaagtat gaatgtagag attgtgacga ataaaaaggc tttagctacc gttcgtaata
     1861 agaaaaaaga actgaaagat ttagacaatc atgcttacca atctgacaat gaaacgaatt
     1921 ccaatgtctt agatgctttg gattccgtgg atgaattgga aaccaccttg gatcagtcaa
     1981 aagaatctat gtacaaactg agttatgtgg ttcgagtgag tgccgaatca gtggatgaac
     2041 taaagcgacg ctgtgatgaa gtgctcgatt tttatgatga taccaatgta aaactagtcc
     2101 ggccctttgg cgatatgatg gggctacatg aagaattctt accctcaagt aaacgatata
     2161 tgaacgacta tattcagtat gttacgtctg attttttggc ggggcttggt tttggggcta
     2221 ctcagatgct aggagaatta gaagggattt actttggcta caatgtggat acaggtcgta
     2281 atgtttacct gaaaccggca ttggcttcac aaggagtcaa gggttcagta accaatgcgc
     2341 tggccgccgc tttcctgggt tctcttggtg gaggtaaatc ctttagtaac aatctcttag
     2401 tctactatgc tgttttgttc ggtggtcaag ctgtgattgt cgatccaaaa ggggagcgag
     2461 gagggtggaa agaaaccttg ccagaaattg cggatgaaat cagtatcctc aacttgacga
     2521 gtaagtcaga aaatcaaggt ctgcttgatc cttatgtgat tatgaagaaa aagaaagact
     2581 cggaaagttt agcaattgat attctgactt ttttaacagg aatttcaagt cgagacgggg
     2641 agaagtttcc tgtgttacgt aaagccattc gaacggtgac tcaaagtgat caacgggggt
     2701 tattacaagt tattaacgaa ttgcgaaaag acgggtcacc aatcgcggaa aatatcgccg
     2761 atcatatcga gtcctttacc gattatgatt ttgctcattt gcttttctca gacggctcga
     2821 ttacccaatc gattagttta gataaacagc taaatatcat tcaagtggcg gatttggttt
     2881 taccggatgc agaaacgact tttgaagaat ataccaccat ggaattgctt agtgtttcga
     2941 tgctaatcgt catttccact tttgccttgg actttatcca tagtaaccga gaaatcttca
     3001 aaattgtcga tttggatgag gcttggagtt ttcttcaagt ggctcaagga aaaacgttat
     3061 cgaataagct tgttcgtgct ggtcgggcca tgaatgctgg ggtctatttc gtcactcaaa
     3121 atagcgacga tttattagat gaacgtttaa aaaataatat cgggttgaag tttgcttttc
     3181 gcagtaccga tattcatgaa atcaagaaaa ccttggaatt ctttggtttg gatcaggagg
     3241 atcaaagcaa tcaaaaacgc ctgcgagatt tggaaaacg
//
LOCUS       DAYYHX010000234         3221 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000234 DAYYHX010000000
VERSION     DAYYHX010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.236"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACHA6Y_002624"
     CDS             301..585
                     /locus_tag="ACHA6Y_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988683.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACHA6Y_002625"
     CDS             588..1004
                     /locus_tag="ACHA6Y_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988684.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACHA6Y_002626"
     CDS             complement(1141..1860)
                     /locus_tag="ACHA6Y_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8988685.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2544..2630
                     /locus_tag="ACHA6Y_002627"
     CDS             2544..2630
                     /locus_tag="ACHA6Y_002627"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8988686.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..>3221)
                     /locus_tag="ACHA6Y_002628"
     CDS             complement(2932..>3221)
                     /locus_tag="ACHA6Y_002628"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988687.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DAYYHX010000235         3143 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000235 DAYYHX010000000
VERSION     DAYYHX010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.237"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..514
                     /locus_tag="ACHA6Y_002629"
     CDS             <1..514
                     /locus_tag="ACHA6Y_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFM8988688.1"
                     /translation="LLELESQEILNKLTEEKQATFKLLEGKDFKDQFVPIVDQEFQEL
                     KAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIEAANLIRETE
                     KDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAEINQKLKEQL
                     QENTEITIQF"
     gene            578..>3143
                     /gene="pglX"
                     /locus_tag="ACHA6Y_002630"
     CDS             578..>3143
                     /gene="pglX"
                     /locus_tag="ACHA6Y_002630"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFM8988689.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKV"
BASE COUNT         1217 a          407 c          591 g          928 t
ORIGIN      
        1 actgttagag ttggaaagtc aggaaatatt aaataaatta acggaagaaa aacaagcaac
       61 atttaaatta ttagaaggaa aagattttaa ggatcaattt gtaccaatag ttgaccaaga
      121 atttcaagaa ctgaaagcaa aaattgaaca agcgaatact atagatgatc ttgttttctt
      181 atcattaaac gtagcagaac agtatttgat ctttaatcaa cgtttcgata atgaagaaaa
      241 ccgcagacgt caagaagaac aagcacgtaa acaaattgaa gcagcaaact tgattcgtga
      301 aactgaaaaa gatgaaatag actctgcagc aaaagctgaa gaaatagcga tagtaaaacc
      361 tactattcaa cagaaaacag taccaatgag aacacttaac gttcatgtta aaactattaa
      421 gtcagcagca gatattgatg agttaacagc tgaaattaat caaaaactga aggaacaact
      481 tcaagaaaat acagaaatca cgattcaatt ctagtgaaaa gacaggcata aaaaggtgcc
      541 tgtcttttca aaggttacat atagaaggga aagaaaaatg gataagaaag cgataaagaa
      601 tttcgcggtc aatgcccgta gaaaattaat tgatgaagta atgactaaag caaaacgttt
      661 aggaattgat gaatcaaaaa ttgaagatgt gaagaaaatt gatagcaatt tacaagaaat
      721 cgaaaaatca ggaataagaa ttcaaggaaa agaaatagcg caaagaaaca aattgattgc
      781 agaactaaaa caacgtgctt ctgtcacttc ttattcagaa gcttttcaag agttaatcga
      841 agaggttgct tatacgtggt ttaaccgatt gattgccatt cggtttatgg aggtaaatga
      901 ttatttacct gacacgtatc gtgtattatc tagtgaaata agtgggaaaa ttgagccaga
      961 tattattaca gatatttatg atgccgatat ctttgaagaa ttatctagta atgaacagca
     1021 acaagtcaga acttggaaaa cagataattc agcagaagca atggacaagc tgtatcaatt
     1081 gatttttata aaaatctgta atcaattgaa tgattactta cctgagctat ttgaagaagt
     1141 agaagattat acagaacttt tgtttacagc ttcttatatt aaaaaagatg gtgtaatcgc
     1201 tgatctttta agtattccag aggatgattt ctatgttcaa gctggtggac aggtagagat
     1261 tattgggtgg ttgtatcagt actataatag tgagccacat aatcaagtgg tgaatattta
     1321 taaagggatt gtcaaaaaac aagatattcc ggcagcaact caactattta ccacagattg
     1381 ggtcgttcgt tatatggttg acaatagttt aggaaagtat tggttagatc attatcccaa
     1441 tagtgaatta agaaataagt tgaaatactt aattccagat aaatatgctt ttgaacaagc
     1501 aacatctgta agtaatttaa aaccagaaga actaaaaatt atcgataacg caatggggtc
     1561 tgggcatatt ttagtttacg ccttcgatgt attgatggaa atttatctta cgttaggata
     1621 ttctgagcgt gatgctgcac gtgcaatcgt tgaatacaat ttatatggtc ttgaaatcga
     1681 taaaagagcg tatcaattgt cttattttgc aattatgatg aaagggcgtc aatacgatcg
     1741 tctttttttg aaaaaaagtc tatcgccgaa tttaagagag tttattgatt ctcctaaagt
     1801 gacagacgag tatcaagagc gtattagtga attgaacaat gataagtata gtgaacgctg
     1861 gaatgaatac aaagagttaa tttttgatca atttgaaaat gggaaagaac ttggatcaat
     1921 cattaagtta gtcgataaaa ataatcaggc aataactaaa gaatacttag gaaaagtaag
     1981 agagtatata tcaaaattca acgaatttac gaatatggat attctttatg ggatgaagga
     2041 aatacaagaa aaatacttgc acattcttga cgtggctgaa atgctagttg ataaatatgt
     2101 agcggtagtc actaatccgc cttatcttaa taaaatggat aagaaattaa aaaaatatgt
     2161 aaatgataac tattctgatg tcaaaaaaga tctattttct gtgtttatta aattgaatag
     2221 ccaaatgctg attgataatg gatatgcttc ctttatgacg ccatttgttt ggatgttcat
     2281 ttctagctat gaaaagttac gctcattttt aattaatgaa agaaacattt ctagtttgat
     2341 tcaaatggag tattcagctt ttgaagaagc aaccgttcca atttgtacgt ttacgataaa
     2401 aaattcaaat gaacaagatg gaagctatat aaaattatca gattttaaag gtggaatgga
     2461 agttcaaaaa attaaaacgt tagaagcaat tcatcagcat ggtgaatgcg attattttta
     2521 tagtactaat caagacaact tcaaaaaaat cccaggaatg ccgattgctt actgggcaag
     2581 cgataacgta atcactgatt ttgctcaagg aaaattaact aacgatgttg cagatgcaaa
     2641 acaagggttg gcaaccggaa ataacaataa atttttgcgg tattggtttg aagtagaatt
     2701 taacaaaata aagtttgatg ctaaaacatt agaggacgct aaaaagtcta gaaaaaaatg
     2761 gtttcctaca actaaaggtg gtcgttttag aaaatggtat ggaaacaatg aatacgtggt
     2821 taactgggag aatgatggag atgaaataaa aaattatgtt aatgaaaatg ggaagttaaa
     2881 atctagacca caaaatctta attactattt taaaaaggga attacttggt cgacactttc
     2941 atctggacaa gtatctttta gattgcagaa tcaggcaatt tttgaaacta aaggatctgt
     3001 tctgttctca aaggatataa ctgactttga agaaatattt ggctatgtaa actctaaatt
     3061 agttaatcac tttttaactt ttatttctcc caccttggat taccatgaag gaccaatggg
     3121 aaaactacct tttcttaaag tta
//
LOCUS       DAYYHX010000236         3115 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000236 DAYYHX010000000
VERSION     DAYYHX010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.238"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACHA6Y_002631"
                     /pseudo
     CDS             complement(24..722)
                     /locus_tag="ACHA6Y_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            801..977
                     /locus_tag="ACHA6Y_002632"
                     /pseudo
     CDS             801..977
                     /locus_tag="ACHA6Y_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1153..1722)
                     /locus_tag="ACHA6Y_002633"
     CDS             complement(1153..1722)
                     /locus_tag="ACHA6Y_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8988690.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1796..2119)
                     /locus_tag="ACHA6Y_002634"
     CDS             complement(1796..2119)
                     /locus_tag="ACHA6Y_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988691.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2273..3085)
                     /locus_tag="ACHA6Y_002635"
     CDS             complement(2273..3085)
                     /locus_tag="ACHA6Y_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988692.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
BASE COUNT          860 a          630 c          547 g         1078 t
ORIGIN      
        1 cagttcaaag agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca
       61 tgaaaatctt gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg
      121 acgtatactt tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt
      181 cctaaagtat tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta
      241 gctagttctt ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca
      301 atgtgccatc cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg
      361 gcgtaatttg ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt
      421 actggtttat cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag
      481 gctagttttt ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg
      541 ccccaaatat tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg
      601 tctaatgtta atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact
      661 ttatagttct tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga
      721 cttttggctc acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa
      781 caccaacatt atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag
      841 atatcaaagg ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt
      901 ctcccgaagt gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa
      961 aaaagcactc aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt
     1021 atttataagc gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct
     1081 taaaggcgtt cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg
     1141 aacatggttt tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa
     1201 ttgagattga ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt
     1261 tcttcttaac tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac
     1321 tccgccaaat ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt
     1381 ttttgctgta atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc
     1441 catcccttca gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc
     1501 ttttggttct ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag
     1561 cgtttcgtat tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata
     1621 tacgccagtt cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc
     1681 tgtgatcgaa gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact
     1741 aatcgctttt tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc
     1801 ggtttttgcc taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat
     1861 ggtacagata cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca
     1921 ctagatctgc ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc
     1981 gatgtcttag cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc
     2041 gaaaaaaaaa gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga
     2101 gtgttttcag taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg
     2161 ctagccgaaa ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat
     2221 aataacgaac ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt
     2281 ggtaaagtac ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa
     2341 acagcgaatc aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta
     2401 tccgtttcca ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat
     2461 ttttttcttt tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa
     2521 agaatagtgt tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca
     2581 ataattggat gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa
     2641 aatctttcaa aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg
     2701 cttttttata agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg
     2761 tagaacgatc gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa
     2821 acgggggtga ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat
     2881 aaactaactg aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca
     2941 aacgtttcag atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag
     3001 cttttcgcca gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt
     3061 ttgataatag tttttgccat ctcatcgatc atttctcacc tgatttctaa acata
//
LOCUS       DAYYHX010000237         3094 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000237 DAYYHX010000000
VERSION     DAYYHX010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.239"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..819
                     /gene="fabI"
                     /locus_tag="ACHA6Y_002636"
     CDS             64..819
                     /gene="fabI"
                     /locus_tag="ACHA6Y_002636"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM8988693.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(929..1981)
                     /locus_tag="ACHA6Y_002637"
     CDS             complement(929..1981)
                     /locus_tag="ACHA6Y_002637"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM8988694.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            2107..3027
                     /locus_tag="ACHA6Y_002638"
     CDS             2107..3027
                     /locus_tag="ACHA6Y_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM8988695.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          899 a          624 c          590 g          981 t
ORIGIN      
        1 tagctattat tctctatttt tttacgacta tcaaattttt atcagaaaca aaggagagtc
       61 attatgtcat ttctagaagg aaaaaaaatc gtcattatgg gcgtcgctaa caaaaaaagc
      121 attgcttggg gttgtgctga agcaatgttg aaacaagggg cagaaattat ctatacttac
      181 caaaatgaac gaatgaaaaa atcattagtc aaattggtcg gcgaagaagc attcactgta
      241 gaatgtgatg tttctagcga cgaaagcatc gaaaaagcaa tgaatgccat cggtgaatat
      301 gctggggaga tccacggcct tgtccatgca gttgcttacg ccaacaaaga agaattgtcc
      361 ggaaatgtgt ctgatatttc cagagacggc tatcaattag ctcaagatat cagcagctac
      421 tctttaattg ctgtatcaaa atacgcacaa ccgtttcttg caccaaacag cgggatcgtt
      481 agcatgactt atctaggttc agaacgcgca atcccgaatt ataacatgat gggtattgcg
      541 aaagcttctc ttgaagctgc tatccgttat ttggcagctg agttctcgcc taaaggaatt
      601 cgggtcaatg gaatctcagc tggggcaatc aaaacattag ctgtcacagg tgttaaagac
      661 tatcaaaaac tgatccagtt atctgaatct cgtacgcctg atcagcaagg tgtgacgatc
      721 gaagaagtcg gtaatgcttg tgctttctta atcagcccac tttccagcgg aatcatcggt
      781 gatattatct acgttgacaa aggtgtccat ctttcataaa cttttgttgc attctttaaa
      841 gtaaaacata taaaaatcag gaaaaaactt tttaaagtct cttcctgatt ttttcttttt
      901 acgttttttt caactcatta ctttgaaatt attccacgac ttcaatccag ccttctggtg
      961 cttctatgtc tccaaattga atacctgtca attcatcata caaccgtttt gttactggtc
     1021 ctacttccgt ttcactataa aatacatgaa gattgttatt gtgataaatc ccaccgattg
     1081 gagaaataac agccgctgta ccacatgcac cagcttctgc aaattgatct agatcatcaa
     1141 tgaaaatatc tccttcaatt gcttgcagtc ctagtcgttc ttcagctaac tgcaacaagg
     1201 aatatttcgt gatacttggt aagatagagg gcgattgcgg agtgatgaat tttccgtctt
     1261 ttgtaattcc aaagaaattt gctgcaccta cttcttcgat tttcgtatgt gtggctggat
     1321 caagataaac acaatctgag taatccagtt cttttgcttc tgttcctgga agtaaactcg
     1381 ctgcgtaatt tccgccgact tttgctgcac cagtcccctt aggagcagca cgatcgtaat
     1441 cagatacaat aaaattcgtt ggttttagtc cacctttgaa atacgctcct actggcgtac
     1501 agaagacgac aaataaatat tctggagctg gatggacacc aatgttttca ccgaccccaa
     1561 tcagtaaagg gcgcaagtat aatgtagcgc ctgttccata gggcggcaca aaatcttcat
     1621 ttgcttttac cacttgcttg acagcatcaa cgaacatctt ttcagggacc attggcatca
     1681 gcaatcgtct agcactttga ttcatccgtc gactattttg ttcaggtcga aagagattga
     1741 ccttgccgtc ttttcgacga taagctttta gaccttcaaa acattgctga ccataatgaa
     1801 gcgctggaga gccttcatga atcgtcaggt aattatcttc tgtcaattct ccttcttccc
     1861 attgtccgtc tttccatttg gcaataaaac gccaaggagt tttgatataa gaaaaaccta
     1921 gattgttcca atcgatttgc atatacttac caccttcgtt attttctcca cattttatca
     1981 ttatccatat tatttgtcat tttattttca gcttttttcc ttcattatag ttatttttgc
     2041 tatttccttt tttgaaaact tcttggtaca atacagaaag aatcaaattt tagaaaagag
     2101 gattttatgt tttcattatt agcaactgcc ggtacgacag actcttctgg cgagacattg
     2161 acagatcaag ctgtcagcaa agtaaatgcg tttcagcggt tttgggacgg gattgattgg
     2221 gacgccattt tggctgcctt tatccaaaaa agtttatatc tgattttttt gatcgtcctt
     2281 ttttggattc ttcaccgtgt gggaaaatat ctgattgata agtcttttaa tcagtattct
     2341 aaaaaagcca ttctaaacga gagccgctta aagactttac atagtctttt aaatactatt
     2401 tttcattata cccttggttt cttttttatc tatgcaattt tatctgctat cggtgtgccg
     2461 atcggatcac ttttggctgg tgccggaatt gcaggtgtgg caatcggtct tggtgcccaa
     2521 gggttcatga gtgatgttat taccggattt tttattatca tggaacaaca gatggatgtt
     2581 ggggactaca tcaagctagc aaacttaacg attgaaggaa ccgtcgtatc tgttggtatc
     2641 cgtacccttc agctgaaagc aacggatggt actgtccatt tcatcccgaa tcgaaacatc
     2701 accacgatca gcaatctctc tcgtgcaaat atgcaagtac ttttagatat tcggattgtt
     2761 cctgaagaag gatatgacaa aatctatgag atcattgatc gagtaaatcg tacattagct
     2821 gaaaaatacc aagaagagct ccaaactgaa ccttctatct ttggattagt ggatcttgga
     2881 aacagcaatt ttgcgattcg tacggtctgc tacgcattga acggaaaaca atatgcctta
     2941 aaagaagagt tcttgagctc ctatgtcaaa gaattgacag cttctggttt cacgattccg
     3001 aacagtccaa ttgctgtcgg aaaataattt ggacaaacga tgcaacgaat ctcactaaaa
     3061 agagcgtggg acaaaagtaa agaacacttt tgtc
//
LOCUS       DAYYHX010000238         3074 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000238 DAYYHX010000000
VERSION     DAYYHX010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.240"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..1081)
                     /locus_tag="ACHA6Y_002639"
     CDS             complement(134..1081)
                     /locus_tag="ACHA6Y_002639"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8988696.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1327..1791
                     /locus_tag="ACHA6Y_002640"
     CDS             1327..1791
                     /locus_tag="ACHA6Y_002640"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM8988697.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1853..2896)
                     /locus_tag="ACHA6Y_002641"
     CDS             complement(1853..2896)
                     /locus_tag="ACHA6Y_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM8988698.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         1016 a          607 c          586 g          865 t
ORIGIN      
        1 tttttgtgaa atttcggctt atttccgaag aagctacttc tggaacacca tttattcgga
       61 aaaaaggact gtgacaagac ttttgtcaca gtcctttttc atttaaaaaa atgtttactt
      121 catttgacgt atattattgg tgaggcttca attcatttgg cttcactttt tctgtttctc
      181 ttatgatata aacaggacgc tgtttcgttt ctaagaagat tttcccaata tatttcccga
      241 tgattccaag acataacaat tggattccac caatgaacag gaagatcgag accatggaag
      301 gccatcctga tgtaggatca ccaaacaaca atgcccgcaa aataatgaac aaaattgcaa
      361 taccagaacc gacacaagac aaagcgccga taaatgaagc aatcgttaac ggtgcatccg
      421 agaagttcac gataccatca atcgagtagt tgaataattt ccagaaggac caagatgttt
      481 cgccagccac acgttcgcgg ttttcataag aaatatattc tgttttaaat ccgacccagc
      541 taaataatcc ttttgaaaat cggttgtatt ctgttaattc taggacggca tccaccattt
      601 gacgagtcat caagcggaaa tcacgagcgc catcgaccat ttctgtatcg ctgattttat
      661 tgatcaactg gtagaatttt ttcgcaaaga aactgcgtat cgcaggctcg ccttcacgag
      721 tcgaacgccg ggtgccgaca caatcgatat cagaagtagt gatccggttg atcatctcag
      781 gaagcagttc gggcggatct tgcagatcaa catccatcac ggtcactaaa tctcctgtag
      841 aagcttgcaa tccagcgtac agagccgctt ctttcccaaa atttctggaa aatgagacaa
      901 agcggacata aggataagct gaggctagct tcttgattgc tgctaacgta ccgtcttttg
      961 atccgtcgtc aataaacaaa tattcgaact catgggcacc tttgaatttt tcaacctcat
     1021 caaaaaaaag aggaacagtt ttttcttcat taaaacatgg gacaacaatt gatactttca
     1081 tcgtttctat cctttcaatc gtatgtttat atatggttta cattcaaaat ttaataaacc
     1141 ttaaataaca cctttaaaat ttgattttac gtaaaagaac aggtattctt tttgtaaact
     1201 aacgtattta gtttaccatg tttcctcggt ttatcaaact tttaagaaca atttttatta
     1261 gttggcaagt agttttggat agagaaaaag gaggaagttc cagtctattg actaggagca
     1321 aacaaaatga aaatacacac acctatcggc tttttatggt tagatgcaga tgaatccgga
     1381 ttgactgctg tatcttttca gccaatttca gaaagcgaat cagcgaatca gcgaatttta
     1441 acgcgcacac aagaacagtt tgaagaatat tttgcgggaa aaagaaaaga atttgatgtt
     1501 cctttatcta taaagagagg tacgcctttt cagcaaaatg tctggaaagc tttgtcgacc
     1561 attccttatg gagaaatccg aacgtataaa gaagtagccg ttgctgttga tagtccgaaa
     1621 gctgtgcgag cgattgggca ggcaaatcga accaatccat tgccaatcgt tattccttgt
     1681 catcgagtga tcgggcaaaa tggaaaactg acaggatata tgggaaacgc aatagatggg
     1741 gttgaaatca aacgccaatt gttgttgtta gaaggatatc tgcaaaatta aaaacagttg
     1801 tgattcggac attgttccat gcgagaatca caactgtttt ttcatgttca ttctaaactt
     1861 tgatcagcca aataaatagt aaagccatca ctgtttggag aagcccgaca gctaatccat
     1921 aagataagaa gcgtgtacct tctttcatga attttgcaaa gtctaaacgc agaccaattg
     1981 ccgctaaagc agtgatctca aaccagctgc tgataaaatg tgccgattgg tcaataacta
     2041 caggtaaaga aaacaagctg ttgaccaaac aaaagatcac aaaccctaat acataccaag
     2101 gaagcggtaa ttttcgtttt ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt
     2161 tcttgtgcat gaatttttca aacagataga caacaacaac gagcatcata atacgtagaa
     2221 tcttaaatag catggcgaat tcgacaacag aattgtcaag taagcttgcc ccagcgacta
     2281 cttgaccaac agattggagt gtaccaccca aaagtgcact tttttcaatg agatttccac
     2341 caaacaactg taatccaaga aaagggagag tcaacatcat cacagtaccc agtagattga
     2401 ctagtgtgat gatctgtcct ttttcttcct ctttcgcatg gatggaaggg gcgattgaag
     2461 caatagcaga agaaccacaa acagcgtttc ctccagccat catcaaagaa gaagtatcgc
     2521 tgaattgcag ttttttacca atcaagtaag caccgatgat agtgactgac atttgcagta
     2581 acacaaaaac aattcctttt acaccaattt gagcaatcgt ttgagctgtg acagttgcac
     2641 ctagtaaaaa aacagagaat tctaatagtt tgctttcagc aaattttgtg ccgattgcta
     2701 gaacaggctg tttaaaaaaa gtatttccta ataaaatacc caataaaata gcaatagtcg
     2761 ctccacctaa ctgtggcaga taaatagcaa gaagcctgct gaaggcagcg atggcaaaag
     2821 aaagcagtaa accgggtaag atttcgcgga ccgctgaatc ctttacagat tttattggat
     2881 gtgacaatga tttcatttga tcggttcctt tcgtcaataa atactaaaag catactcgaa
     2941 catagtagca ctaaaccagt attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt
     3001 acgacctctt gtttcgaata aacggtgttc cagaagtagc ttctacggaa ataagccgaa
     3061 atttcacaaa aatt
//
LOCUS       DAYYHX010000239         3036 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000239 DAYYHX010000000
VERSION     DAYYHX010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.241"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..959)
                     /gene="murB"
                     /locus_tag="ACHA6Y_002642"
     CDS             complement(24..959)
                     /gene="murB"
                     /locus_tag="ACHA6Y_002642"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM8988699.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1032..1952)
                     /locus_tag="ACHA6Y_002643"
     CDS             complement(1032..1952)
                     /locus_tag="ACHA6Y_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8988700.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2065..2817
                     /locus_tag="ACHA6Y_002644"
     CDS             2065..2817
                     /locus_tag="ACHA6Y_002644"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM8988701.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          855 a          612 c          526 g         1043 t
ORIGIN      
        1 cttattttaa gttccattac ttattaattg gctaaacttc tgcttgtttc tgatattgct
       61 tgtttttctc caatgatccg cacttctgtt tctaaagaca catcaaattt ttctttgatt
      121 acttcttgga tgtgcgcaat caattcaata taatctgtcg ctgtcgcatg atcgatattg
      181 acgatgaatc cagcatgttt ttcagagact tgtgcaccgc cccatttcag gccttgcaat
      241 cctgcttgtt ggatcagctg acctgtatag tgtcctactg ggcgcttaaa gacacttccg
      301 catgaagggt attctaatgg ctgttttgat tgacgcaatt cggtcaactc atccattctt
      361 gctttgattg cttcataatc ccctgtttgc aaactgaatc gagcttccaa tacgatagca
      421 cgtaattctt ggactttact gtgacgataa gaaaatacca tttcgtcttt tgtcaaagtt
      481 ttcaatgttc catcttcaag caaaagatcc acttcagcaa aaacatcttt gatctctccg
      541 ccatacgcac ctgcattcat ataaaccgct ccaccgacac ttcccgggat gccgcaagca
      601 aattcaaatc cagtcaatga ttctgccaaa gctgcataag tcgtatcaat cagtgaggca
      661 cctgcttccg caatgaccat cgttcctttg acatgaatct gtttcatttc agtcagcatg
      721 atgactacac cgcgaatccc gccatcttga acgatcagat tgcttgcatt tcctaatacc
      781 atccaaggta tatcatggat tcggcaataa tcgatgattt gctttacttc ttcttgtttt
      841 ttcgggaatg caaggacatc agctggtcca cctgttttcg taaacgtata gttcatcaat
      901 ggttcatcaa acaatatagt aatcgtggct aagctttcaa ctatttcttc tttattcact
      961 gaggattttc ctttcttttc tcaaacgtcc tcattattct atcacgctta atacaatctt
     1021 gccagtcttg ttcaaagttg atgacggtag tgatctttaa gcacttcctc acataattca
     1081 tgacttctta caacttcttt ttgtttaagc aattctgttt ccgatttacc taaacggatc
     1141 tgagaaaaga aggcctctac cattggctca aacccgcgtt tgactagcgt gtttgtccag
     1201 tcgccaaatt ctttcacttt ttttccatca gcatcttgaa tgacaagctg ggtcagatcg
     1261 tttacttcat aagtaccatt tttacttgtt acttgatatt tttctatatt agcgccactt
     1321 gctaaatcca tcgttaaaat agccgtttga ttttgtgttt ctaattgtaa aatggcatat
     1381 tccaaaaatt cctttgtttc tcttatctgg tattttgttc tcaaaatggg ttcatctaat
     1441 aaatacacag ctgtatcaat aagatgtaaa aacaaatcat aaaggacgaa ctctgtcgtt
     1501 tcttttgccg ctattcgatt tttttgcagg tggatcaacc gcttatctgg gatacgtttc
     1561 aattcttcta ccataggagc aaagcgccgg ttgaaaccga tcatcaaaat tatttggttc
     1621 ttatcagcta attcttgcaa ttcacgaact tcatttagat ggacactcag tggtttgtca
     1681 atataaacat ggataccatt ttctagacat tttttggcta attcataatg tacttttgtt
     1741 gctgcgtgga tcatacatgc ttcgattttt tcttctaaaa gctcatcgat cgtttcatat
     1801 agatgttgga acccgtactc tttttgaatt ctttcttttg tttctttatt tcttgttgca
     1861 aaataaaaat caagtgttcc ttgttttttc gcataagttg gtaaatatgc tttttctgcg
     1921 atattcccta caccgattac accaatcttc atctactttc ctcctggatc atttagttac
     1981 tttcatctta caccattttt tactaaaatg aggtacaata gtatcgcaaa ggtaaggaaa
     2041 ttaattcgaa ggagtgtgct tttattgaaa tttatctctt ggaacgtaaa cgggcttcgt
     2101 gccatcgtaa ataaaaattt tttagaagtt tttcatgagt tagacgccga ttttttctgt
     2161 ctgcaggaaa cgaaactcca agcaggacaa attgatttag atcttccagg ttattatcaa
     2221 tattggaatt acgcggaacg aaaaggctat tccggcacag cgatttttgc taaaaaacca
     2281 gcattgaatg ccacttacgg aatgggtatc gatatccatg atacagaagg ccgtctgatt
     2341 actttggaat attccgattt ctttcttgta acctgttaca cgccaaattc tcaaagcgaa
     2401 ttaaaacgtt tagattaccg attagaatgg gaagaagcct tttataatta tttggaaaat
     2461 ctaaaaaaac aaaaaccagt catcgtgtgt ggggatctaa atgtcgctca tcaaaagatt
     2521 gatttgaaaa actggaaaac gaatcaaaaa aatgctggat tcactccaga agaaagagcg
     2581 gctctttctc gactgttgga taacgggttt atcgatacat tccgttattt ttatccaacc
     2641 caggaaggtg tctactcttg gtggaactac cgattcaatt caaggaaaaa caatgctggt
     2701 tggcggatcg attacttttt gacgagcaaa gatttagagc caggactagc tgatgcaaag
     2761 atccatacgg acattatggg cagtgatcat tgtccagtag aattagattt gaaataaaga
     2821 ggttgtgaaa aagtcgttta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc
     2881 acattttgca taaatttggc ataataccga gaaactggct tttgaacact gtttatcgag
     2941 agattgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
     3001 catatttttc gtttggttgg gcttattacc gcagat
//
LOCUS       DAYYHX010000240         3003 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000240 DAYYHX010000000
VERSION     DAYYHX010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.242"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1446
                     /locus_tag="ACHA6Y_002645"
     CDS             <1..1446
                     /locus_tag="ACHA6Y_002645"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8988702.1"
                     /translation="KNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTIAN
                     IQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLKEI
                     STLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVRVE
                     IDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSEQL
                     SSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQIT
                     PHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHINDYL
                     VILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITTCK
                     IDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGNDQK
                     PITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            1439..>3003
                     /locus_tag="ACHA6Y_002646"
     CDS             1439..>3003
                     /locus_tag="ACHA6Y_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8988703.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
BASE COUNT         1005 a          592 c          526 g          880 t
ORIGIN      
        1 aaaaatttaa gcataaaaca gatttttgcc caagcaaaca aagccatgct tctatttgct
       61 gttatccctt tgctactgag catactgtta tattcgcaac agcttttgat ttatcaacag
      121 acgatcgcaa atatccaaaa agcagatgca attgctttga aggttgatca aaaagttttg
      181 gaaagtatgt gggacttgat ttctggccaa gtaaccgtag atgagttcaa tgaggaaaat
      241 atcctagaag aacttaggaa tgatattcga gagatcaaaa ataatacaaa tacacttaaa
      301 gaaattagta cgttagatgt atcgctacgt acgatcgata cactggaaga ttatcaaaac
      361 gagctgatca ataatatctt ggcaaaagat tttatggata ccaatgaaga gatcatcaat
      421 caggtagatt ctgtcactca actgctgtca gatatcttac aagattttgt tcgtgtggag
      481 atcgacttag caagcaaaaa aaatacgaca cttgtccgct cgctcattct attaacaata
      541 gtggaacttg gagttcttag taccattttt ttctatgcga aaaagaatcg tcgcttttta
      601 gagagaaagg tccaagaacc gatcaatcag ctgatcgaga tgtcagaaca actttcttca
      661 gggaaccttg gctttcgttt aaccctgccg gaaacactgg aattacaaac acttaccgct
      721 agcatgaata aaatggcgga agatctaaac cagtctttac gggagaacgc tttgaaacaa
      781 taccatttag cacaaagtga agttcgtgta ttgcaagctc agattactcc ccattttatt
      841 tataacagtt tggatgccat tatttctttg atcgaacaag actgctttga agaagctctg
      901 actatgaccc atgcactttc cggctttttt cgtacgtctt taagcaaagg acaagactgg
      961 attcctcttg aaaaagaaat tagccatatc aatgattatc tagtcatctt aaaaataaga
     1021 tacggccata tgcttgacta taagatcgat ataccagaaa cactttttcc tgtttctatt
     1081 ttaaaaatga tcttgcagcc attgattgaa aatgctgtat accatggaac gaagtacgtc
     1141 cgtcacaaag gatttattcg tattaccaca tgcaaaattg atgagatgat ccaattcaaa
     1201 gttatagaca acgggattgg catccctgca gaaaaactgg cagaaatacg taaagagtta
     1261 gccaaaggga tcgattcaga tttcagtatc ggctacggac tttacaacgt aaacaagcgt
     1321 ctccttcttt attacggaaa tgatcaaaaa cctatcacga ttgacagcaa ataccgagaa
     1381 ggaacaactg tcacactgat gatccctcta tctcccaaaa caattggagg aaacaaacat
     1441 gtataaagtt tttatcatcg aagacgaata cctcattcgc aataacttgc gggaacaaat
     1501 aactgtcctt ggttcttatt atccaatcac ttatgtcggc gaagctggag atggtgagat
     1561 ggcactgtct tcaattatcg acttacagcc cgacatcatt ctgactgata tccaaatgcc
     1621 tttcatggac gggttgactt tttcaaaaga agcaaggaaa ctacttcctt ggactcgtct
     1681 catatttatt agtggattcg acgatttcga ttatatgaaa ggagcaatcc aggtacaggc
     1741 agatgattat ctgcttaaac cgattaaaag tagcgaattg aaacaagcaa tagaaaaagc
     1801 agtaactgct ttagatttgc agcaaaaaga aacgcacact tcttctgatc ttgttttcga
     1861 attgaaaaaa aatcatttct tgaacggttt gttcaaaggc tcacttacga tcagtgaaac
     1921 attgaaactt gctgaatctt tcaatcgctc tttagcaggg aaaaaaatga ccgtattatt
     1981 agcagcaaac caagtcagta cagactttga agattacgct catctcagtg attatttgaa
     2041 gtttttattt agggagaatc aacaagtgat cttctctgct gtttcctctc gattcatcaa
     2101 atttttaata gttgattctc ttgaagaaaa tgtgatcgag accagttatc aggtagccca
     2161 aacgttggtc catgaattag aaaagggaca aaaaaataat ttttctgtcg ccattggtcc
     2221 tgtcgcagaa agaatcagcg aattacctgc atcttttgag atcacgcaga tgatacttga
     2281 aaccgccagt tcccttaaag aacaaattat cagctatgag gatttgattc aagaagcgaa
     2341 acttccagtt gattctgtct tttactttga cctctcactt gatttgtctt cattatcgaa
     2401 aaacgaagta tctaagtaca tcgaacactt gacgaaacca caaggcacac caactcgaac
     2461 acgattatat cgtttatttg tcttgactaa gctactccag catgtctgcc tgaaagaaaa
     2521 gaaaatcccg caagaaatgg ttcattgcac atccaccagt caacgcttag caatcagcgc
     2581 cgacgactcg ctttaccgtt cgacagttta tatgttcatt tctttcttaa ctgctctgcc
     2641 ctcacagcct tcaaggaaca aatatcagcc aattttgaac catgcattgg attttatcaa
     2701 agaaaatttt accgatccag atatgtcctt aacttgggtc gctcaacaag ttgcattaag
     2761 tccctctcat ttcagtacga ttttttctca atcgtttaac caaacattca tcgattatct
     2821 gacagaacaa agaatcgaat tagcaaaaaa attattgcag caatcagact atcgtatttc
     2881 agatattgct tttgaaattg gttataatga ccctaactat ttcagttatt tatttaagaa
     2941 aaaacaaggg atttctccaa aagaatatcg gacacagctt ttccttaaaa acaaaaataa
     3001 cta
//
LOCUS       DAYYHX010000241         2979 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000241 DAYYHX010000000
VERSION     DAYYHX010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.243"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1617
                     /gene="alsS"
                     /locus_tag="ACHA6Y_002647"
     CDS             <1..1617
                     /gene="alsS"
                     /locus_tag="ACHA6Y_002647"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM8988704.1"
                     /translation="INHHVDYVFGIPGAKIDGVFNELEDQGPELIVTRHEQNAAFMAQ
                     AIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAGQVKRSDLLKLTHQSMD
                     NAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFISIPQDVVDAPVQGNVI
                     KPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKETLAIRQLVGKTALPVV
                     ETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAIGYDPIEYEARNWNAEK
                     DARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQQVSEDAKEYLASLQAE
                     LTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSHYIWMARHFRSYEPRHL
                     LFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQDLETAVRKKLNIVHLI
                     WNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGIRATSVEELEKALEEGF
                     ATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1630..2340
                     /gene="budA"
                     /locus_tag="ACHA6Y_002648"
     CDS             1630..2340
                     /gene="budA"
                     /locus_tag="ACHA6Y_002648"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM8988705.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2470..2967
                     /locus_tag="ACHA6Y_002649"
     CDS             2470..2967
                     /locus_tag="ACHA6Y_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM8988706.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT          982 a          501 c          674 g          822 t
ORIGIN      
        1 attaatcatc atgttgatta tgtattcggt ataccaggtg caaaaatcga tggtgtattt
       61 aatgaattag aggatcaagg tcctgaatta atcgtgactc gtcacgaaca aaatgctgca
      121 tttatggcac aagcaatcgg acgtattaca ggagagccag gagtagttat cgcaacaagt
      181 ggtcctggtg ctagtaactt agctacaggt ttggtaacag cgacagcaga aggtgaccca
      241 gttttagcaa ttgccggaca agtaaaacga agcgatctat tgaagttgac ccatcaaagc
      301 atggataatg cagcactgtt ccagccaatc acaaaataca gtgcagaaat ccaagatcca
      361 gaaacaattt cagaagtgat tgccaatgct tatcgtatgg ctaagagttc aaaaaaaggc
      421 gcgagcttta tcagtatccc gcaagatgta gtggatgcac ctgtccaagg gaatgtcatc
      481 aagccattga gcgatcctaa attaggttct gcttctgcag atgatatccg ttacctagct
      541 gaacgcatta gagaagcaaa actaccggta ctattagtag gtatgcgagg atcaagtgaa
      601 aaagaaacgt tagccatcag gcaattagtt ggaaagacag cattgcctgt tgtagagaca
      661 ttccaagcag ctggagtgat ctcacgtgaa ttagaagcgc atttctttgg tcgtgtgggt
      721 ttatttagaa accaaccagg agatatgctt ttgaaacgaa gtgatctagt tattgctatt
      781 ggctatgatc caatcgaata tgaagcaaga aactggaatg cggaaaaaga tgcgcggatc
      841 attgtgatcg atgaagcgcc agctgaaatc gatcctttta tgcagcctga aagagaattg
      901 atcggtgata tctcagccac tcttgattta ttgacaggtt cattagagcc tcaacaagtg
      961 tcagaagatg caaaagaata tttagcatct cttcaagcag aattaacgga gcgggacatt
     1021 gttcaatcaa aaggagaagc aggtatcttg catcctttag aagttattaa cacgcttcag
     1081 tcaaaagtaa cagatgatat gactgttacc gtagatgtag gtagtcatta tatttggatg
     1141 gcacgccact ttagaagtta tgaacctcgt catttgttat tcagtaatgg tatgcaaacg
     1201 cttggtgttg cattgccatg ggcgatttct gctgcattgg tgcgtccaaa tacacaaatc
     1261 gtatcagttt ccggcgatgg gggattttta ttctctgccc aggatttaga aacggcggta
     1321 cgtaaaaaat tgaatatcgt tcatttgatt tggaatgatg gacactacaa tatggtagag
     1381 ttccaagaaa aaatgaaata tcagcgggcg tcaggtgtag actttggtcc agttgatttt
     1441 gtgaaatatg cagaagcttt tggcgctaaa ggaatccgag ctacaagtgt agaggaatta
     1501 gaaaaagctt tagaagaagg atttgcgaca gaaggacccg taattattga tattccgatt
     1561 gattaccgtg ataatgagaa attgggagaa acaatcttac cagatcaatt ctattaagga
     1621 agtgtcgaca tgactgaaaa aatcttatac cagcacggga cattaggagc tttgatggct
     1681 ggcttgatgg atggaacaga aacgattgct catatattag aaaaaggaac tctgggattg
     1741 ggaacattgc atgggcttga tggtgaagtc atctttttag atggcgtagc ttatcaagga
     1801 agatcagatg gctcagttgc ccaattagat ggaagtgaac ttacccccta tgcagcaatt
     1861 acagatttta cgccagatac tagtttttct gtctcaaaaa ctgcagatgg agaacaattg
     1921 aaaaaagaca tattagtaag agaagccggg gaaaacctat ttttggcagt gaagataact
     1981 ggattattca aaaatatgca catccgtatc atgccgaagc aagaaaaacc ttacagaaga
     2041 ctagcaaaaa tttcagagtc tcaaccagaa ttccaacaaa gcaatgtcca aggaacatta
     2101 gtcgggttct ttacgcctga attattccaa ggagttgctg cagcgggatt tcacttgcat
     2161 tttatcgatg atacacatac atttggcgga catgtaatgg attttgaagt ggctgaagga
     2221 aaagttgaga ttagccgcat ctcgtccctt gtacagcatt ttccagttga tgatccgact
     2281 tttgttcacg aaacgattga ttatgctgat ttagcagcag aaatccaaca agcagaatag
     2341 aaaaagacag cgagatgcgt tcaaggtgat taacctgacg tatctcgttt ttttgaggag
     2401 gaaacctagc gtttacaaaa aaactgagct ataatcttct ccatagatca aaaaggagcg
     2461 gtttttatca tgtggacaga aaaacagaaa aaagatgcat acgaaatttt attattacag
     2521 caacaaggat tattagaagc agaagataac tggttagcaa atttagcaaa ttcatctgca
     2581 ttgttgaatg agactttgcc tgagactgtc tttgcaggat attatttatt cgatggtaat
     2641 gaattgattt taggaccgtt ccaagggcga gtttcttgca cacgtatcaa gatgggaaaa
     2701 ggagtttgtg gagaatcagc tgaaaaacaa gaaacattga tcgttgatga tgtcaaaacc
     2761 catgcaaatt acatttcctg cgattcagcc gcacaatcag aaatcgtagt gccaatggta
     2821 aaagaaggta atttaatcgg tgttttagat atcgattgcg gagtaacgaa agggtatgat
     2881 gaaatcgatc aagcatttct agaaaaatat gtagatcttt tattagcaaa ttttaatata
     2941 aatgcttttt catttattaa taactaaaat cgtctattg
//
LOCUS       DAYYHX010000242         2973 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000242 DAYYHX010000000
VERSION     DAYYHX010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.244"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..403
                     /locus_tag="ACHA6Y_002650"
     CDS             167..403
                     /locus_tag="ACHA6Y_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988707.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            410..2362
                     /locus_tag="ACHA6Y_002651"
     CDS             410..2362
                     /locus_tag="ACHA6Y_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8988708.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2434..2931)
                     /gene="dfrG"
                     /locus_tag="ACHA6Y_002652"
     CDS             complement(2434..2931)
                     /gene="dfrG"
                     /locus_tag="ACHA6Y_002652"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8988709.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1046 a          496 c          532 g          899 t
ORIGIN      
        1 tgatactcgt tttttattgt taaatcaacg tttgttgtta atttacacca caaaatgtaa
       61 gttaaaatgt atataaaaat gcgaacgtga aataattatt gtaggaatgc aaattggtgt
      121 gttaaaatgg ttacaattta ctaagcaaaa aggggtgcat tttcctatgc aatttcttga
      181 gccaaaaaat aagaacgcta agtctgtgga ttgggaaata tcagagcaag taagggtgat
      241 agttaagcaa tacgctgaat atgcagagcg tactgaaagc gaagcagtgg atgaattctt
      301 attgaacatt ttagacgata agaagtttat tgaatggatt gccaacaagc gaagtaataa
      361 gcggattgtt gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa tgaccaagct
      421 taaaagatta gccacgaagg aagatgaaat tgtagatgtt aaaattccga tttcaaacga
      481 agaattaaaa gatagggcta aacaatatga tttattaact ccaaaacgat ttgcaacaag
      541 gtataacaaa atgttgttct tgccaacgtc ttttaaatgg aacggttctg aatatcccat
      601 tcaatataat tactgcatca atccgttttg ttgtaatttt gggaaagaac agcacaaatt
      661 caaagatgta aaagggaaac caagtcgata taaaatgact ggtagttcca aagataaagg
      721 tcataaaggt atgtactgca atgacaatcc tattggcaga ggtgtatcgc aaaactgtac
      781 agtaacccct ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa
      841 tagcatccaa gatgttgagc cagattatca atttcataaa gaaggctgct cggaagaaga
      901 atcaacccca ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc
      961 tcaacgctat caatgtaagg cgtgtaagaa gttcactaat gttctaccaa aaagagaaga
     1021 aaccactact tatcaccaac aaaagaacac cattcttcca atgttcgcta agatggttgt
     1081 tggaagggtt tcagtcagtc gtacttgtga tattttagga attggagttg gaacctacta
     1141 tcataagctg gagtggctgt ataggcgttg tttggagttt ttagaacgat atgagacaca
     1201 accccttcaa acaaagaaat ttaacgagat gtggcttaat acggacaaga tgcactacta
     1261 cctaaataac gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt ttgaagacct
     1321 taatatgcaa acgtatatag ttgtatctgc tgaggtctta tcaagatatg tgttccgttc
     1381 tgatgttgcc tatgattgga atatttcaat ggatgaatta aatgaagata caagaaaatt
     1441 caaagaagac catctcaata cttttagtcg taaaaatgat aggttagatt ggtcttatta
     1501 tccacaagaa ccatccgcta acgatagcga aaataggaat gcttatcttc acgaattagg
     1561 gaaaattact aataggagta gatttgtcga tggtttaaat gttgatgctc cttatactac
     1621 aactgctcac tattggttaa ttaaacaaat ggttaatgcc gacgaatggc gaatgattag
     1681 tgatgatgac ttctctatta gaaacgcctt ttatagagta ttcacgaaag aattaaggtt
     1741 gagcgatgca catcatttta tttgccaagt taacaagacg aaaagcagaa aacaatgctt
     1801 aaaagaattc ggacaagcaa aagcagaact gctcgattgg ggagatatca gaggattcaa
     1861 aacaaagttt ctacggactt tggcatctca ttatctaact gaattactaa caagtcatca
     1921 attccacgaa gaggctatta gtaaagatgg agaaagatat cgcaaatatg ccgataaccc
     1981 tatcaaacat ccattagcca ctaaggataa aggattttat tcagttgatt gtagaacgga
     2041 tttatctgct ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta atgaccattc
     2101 gaccaatagc tttattcaac agataagacg ttatatatca tctcttgaaa gaccattaac
     2161 aacagctcgt ggtgataaga agagctatat ttacgctaac ttcaatccga agtatgctca
     2221 atttgcgatt acaattctaa gaacttacta caacttttgt cgccctttta aatcagcgga
     2281 taaaaaggtt ttgacacctg ctcaaagatt agggattaca gataaacaat ttgattggaa
     2341 agatataatt tatttcaaat gacaaaaaga gcttggagta atcccaagct cttgtgcttt
     2401 atctttactg ttcttcttca attaacgcac ccgttaactc aataagtttt ttctttcata
     2461 tacatgaaaa tagtagttat acggattttt atcattcttt atcccttttt gggcaaatac
     2521 ctcattccat tcctcataat tcacttctgg aaaaaaagta tctccttcga attcatgatg
     2581 tatttttgtg atgtacattt tctcaacata agggaaaaac aaattataaa tctgttctcc
     2641 tccgaaaata aaaatttctt cttcgttttt acataactca aaaacatctt ctattgaatg
     2701 aacaatttca caaccattaa aggtaaaccc cttatctctc gtcagaataa tatttcttct
     2761 gtcaggtaag gctcttccga ttgattcaag gttcttccta cctaatatta tcggatgtcc
     2821 ctttgtagta tttttaacat attcccagtc cttgggaatc ctccaaggaa tgtcattctc
     2881 tttgccaatc actctattct tatccatcgc agcaatcaaa gaaactttca tttataacac
     2941 agccttttta actaatttac catctaattt ctt
//
LOCUS       DAYYHX010000243         2938 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000243 DAYYHX010000000
VERSION     DAYYHX010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.246"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..112
                     /locus_tag="ACHA6Y_002653"
     CDS             <1..112
                     /locus_tag="ACHA6Y_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8988710.1"
                     /translation="KGMETIRGIYKKNRRNGTLFGFSVSTEIKVLMGITA"
     gene            complement(169..600)
                     /locus_tag="ACHA6Y_002654"
                     /pseudo
     CDS             complement(169..600)
                     /locus_tag="ACHA6Y_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1159..1476)
                     /locus_tag="ACHA6Y_002655"
     CDS             complement(1159..1476)
                     /locus_tag="ACHA6Y_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8988711.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1477..1731)
                     /locus_tag="ACHA6Y_002656"
     CDS             complement(1477..1731)
                     /locus_tag="ACHA6Y_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8988712.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1991..2215)
                     /locus_tag="ACHA6Y_002657"
                     /pseudo
     CDS             complement(1991..2215)
                     /locus_tag="ACHA6Y_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2769..2855)
                     /locus_tag="ACHA6Y_002658"
                     /pseudo
     CDS             complement(2769..2855)
                     /locus_tag="ACHA6Y_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          964 a          527 c          440 g         1007 t
ORIGIN      
        1 taagggcatg gagaccattc gaggaatata taaaaagaac cgaagaaatg gaacgctctt
       61 cggcttttcg gtgtctactg aaatcaaggt attaatggga ataacagcct aagatatttg
      121 gagttcagag agggcgcgtt tgattttcaa acttcgcaac agaaccttca tatttccttg
      181 attacctgag gcaatgaaag acttatctac ttgaattagg gtgaaattct cattcaaata
      241 tggtagtaaa attaatatat gtgaaaatcg aatcttgaga tagctattcc ccttgagata
      301 ttcactgata cttctactca aatcatttaa atttttataa ttatttgtac ggaacgtata
      361 tatatcaaca tcagttttct ttacatgaga acttattcta aaatgaaaaa gcctactctc
      421 agaaagaatt gaaaaataga tactttcagt agatactgaa gattgaacaa aattaaactt
      481 gtatccttca ggcatagcac ttttaacagt ctgctgcatt aactcggata atcttttgaa
      541 agtagtaata tattttttac cagcaaacca tacatatctt ttttcattaa gtatgttcac
      601 tatctctcct ccattaatta tatttagcat caaatagtta ttgtacctat attttatccc
      661 attcctttta aaacaagaat acgtacaaag taaaatgttg taaatagtct agcagggatg
      721 tatattttca tgtaccaaca taatatttaa gaagtttccc atagatatat tggacttcct
      781 cttttaaaac acaaaaataa atactacctt atttaggtag tatttattaa aagttacgtc
      841 aattttgatt aaaaatatac tacttgaacc tctcatgagt aaagtcacga aatttttggg
      901 aatagagcgt acttgatatc ttattttttc actaatagca gtgtctctta ttcaccaagc
      961 gatccccgta gttcctacgg ttcttaattt ttctctagac taatgattgt atctttagac
     1021 cttcggccat aacatggagc aaaataaaaa ggtactaagt cttatatatc aatcttcgag
     1081 taaccgacgg ttaacaacca tatcgagcgg cattaatcac ccacctgtag aggatgagcg
     1141 acttttgcct aattattgtt aaaagcctag tttcatttgt ttagattgga aaatacgtcc
     1201 tacaagtatc tctttttttt catggtcgat ccgataaaat aaggcatagc ttttgccgat
     1261 cacgatccga tatgttggga tatccattcc atagatttcg gaaacttcct catgcatagt
     1321 tggaaaattt ttggttagtt ctaaagcctt ctggatcgca cctacaaatc tttttatgct
     1381 ggctggagaa agagcaagct cattttccca ttcttctatt atcgtttcaa ggctggtctg
     1441 gaaactttta gaataaacta gcgtgtactc tttcatctat tcactccagc cgtattttcc
     1501 gaaagctttt accatttcaa tttctgaaac tgttaaacct ttctcaaagt cttggaaact
     1561 ttcttcaatc aatttgcgat cgtgttcttt tgctatttga gtagtatcta atatgaataa
     1621 gttgccttct tgtttggctt ggatagtgtc tccgcttttt aatccaagag agcttggaag
     1681 gatcactccc acgctattgc cgatctttct aacctttagt tcttttccca tgttatcaca
     1741 cttcttttag tttttgataa ataaattata acatgttata acgacaattg ccaacggtgt
     1801 ttcttaaggg attccactcc atattattat tggacaaaat aagccactta gaacgctgaa
     1861 aacatataca aacgatatca aaatacgagt gaactactca ccatacactt ccgttaacat
     1921 ccatttaagt aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat
     1981 atagctaaag ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt
     2041 tctcggctaa ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat
     2101 tttcttgaat ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc
     2161 aattacctga taaacctcca gttttttgct cttacttaaa tggatcaata gcaatttcat
     2221 cacaaaccta tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa
     2281 ctctatgtag caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct
     2341 aaaaatatat actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa
     2401 atcaatacat aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat
     2461 agttttgctg tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat
     2521 acctttttga ttttgttttt gcaatcaagc catttgcaga ggtaactgca cactacatct
     2581 gcaagtggct tgattgcaaa attgccaaaa aagacagtaa gcctaccagt taattctata
     2641 atatagaaac gtaaaaaaaa ccccatcagc ctacacgctg atggggtttg atgatataat
     2701 gaaatacctc tttgttactc ataattatac cataattagt cacgaatgca accaatagaa
     2761 aaaactactt agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt
     2821 tgtgaagcat taaatacatt gttattaatt gtcgctgttt ttaactaggc ctctcccctt
     2881 ctattataaa agaagatata tattatttct taattaccta ctaccttaaa ccaaaatt
//
LOCUS       DAYYHX010000244         2932 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000244 DAYYHX010000000
VERSION     DAYYHX010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.247"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      191..365
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            503..2095
                     /locus_tag="ACHA6Y_002659"
     CDS             503..2095
                     /locus_tag="ACHA6Y_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8988713.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            2108..2848
                     /locus_tag="ACHA6Y_002660"
     CDS             2108..2848
                     /locus_tag="ACHA6Y_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8988714.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT          920 a          538 c          637 g          837 t
ORIGIN      
        1 catttttgtg tataattata ctatcgtttg caatggatag atgattcaca ttttgattgt
       61 tttttcggta aaagtaaaga tttctatatt tatcagtatt ttttcaaaaa ccagcagtta
      121 tagattgaca aagcctctcg agtatcgtaa actttttttg tataaataac tgaatacgag
      181 atgcctcaat caatgaggta gaggtcgcga gaatcattaa ccctgtggag tgaagcaaac
      241 atggagaagc aggaataggg aggatcgccg aaatgtgcac ttttttgctt ggagtgtatg
      301 ttgggacgca agttaagagc ttgcggactg tcttagcagt gatgctaagt tgcgctatgt
      361 tttgtgagag gtttatcacc ttatggaatt ttagcggcct ttttatatct ataaatcggt
      421 cgctttttat tttttcagat aaagctttac cgcatttcat ctagaccgct cgtgtatcat
      481 ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt tcagttgccg tactatttgc
      541 ttttgtcttc tcactattca gtgtgggatc tctggcaaat gcagatgaaa aaacaccgag
      601 cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc ttcaactggt ctcaacaaac
      661 agatgagaat aatgccttgc caattcaagg ccaaaatagt tacgccggcg gttatgatgt
      721 tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa ttagtcatcg tccaaacgaa
      781 atgggatggt ctggctcctg cccttcaatc aggcaagatc gacgcgatca tcgccggaat
      841 gagccctact gctgaacgac gaaaagaaat tgattttacc gatccttatt acgaatcaca
      901 attagtagta gtagttcaaa aagatagcaa atatgcaaaa gcaaaaagtt tagcagatct
      961 ttctggagca aagatcactg ctcagttaaa tacattccac tatactgtga tcgatcagat
     1021 accagatgtg cagaaacaac aagcaatgga taatttctca gcaatgagaa cagctttagc
     1081 ttctggaatg atcgatggtt atgtcagcga acgtccggaa ggtgtaacgg caactagtgt
     1141 gaacaaagac ttggaaatgc ttgaattcag cggtgcagat ggcttccaga caaacccgga
     1201 agatgttcag atttctgttg gtatgcgtaa aggcgacccg gatatccaaa aagtaaacca
     1261 gattctttct ggcatctcta acgatgaacg aaccaaaatt atggaccaag cagtcaaaga
     1321 tcagcctgct gcaactgata gtgaagaaaa acctggcatc atttctgatt tcactacgat
     1381 ttggaaacaa tacggcaata tgttcttacg aggtgctgga cttaccttgt tcatcgcatt
     1441 gatcgggaca gttgtcggta ctacacttgg attactgatt ggtgtcttcc gttcggtacc
     1501 tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc ggaaatgcac ttctttctat
     1561 ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa gcaatggtca tcttctacgg
     1621 tctggcactc gcttttggca tttcattgaa ccgaacgatt gccgctttgt ttattgtctc
     1681 tctcaatact ggagcttaca tgacagagat cgttcgtggg gggatttttg cagtagatga
     1741 agggcagttc gaagctgcgc aagccattgg tatgacccat ggccaaacga tgagaaaagt
     1801 tgttattcca caagttttac ggaatatctt gccagcaaca ggaaatgagt ttgttatcaa
     1861 tatcaaagat acagcagttt taagtgtaat cggcgtagct gatttgttct tccaaggaaa
     1921 ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt acgatcgtcg ggatcatcta
     1981 cttagtcatg acctttacga tcaccagaat cctgcgggcg gtagaaaaga aaatcgatgg
     2041 tccttcagct tataccaagc ttgaagaaat cgacaattca aatttgcagg aataaggagg
     2101 aagaatcatg aatgaaaaaa ttattgaaat caaacatcta agaaaaagct tcggtgaaaa
     2161 tgaagtattg aaagatatca gcgtaacagt caataaaggt gaagttgtga cgatcatcgg
     2221 ttcttccggt tctggtaaat ctactttctt gcgctgtatc aatttactgg aaaaaccaac
     2281 aggcggcgag atcatttata atggagaaaa tgtattagct cctaaatata atttacctaa
     2341 atatcgaacg aatctaggta tggttttcca atccttcaac ctgttcaaca atatgaatgt
     2401 cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa cgagataaag aaactgccaa
     2461 aaaagtagca atcgaaaatc tggaaaaagt tggtatggat cgttatatcg aagccaaacc
     2521 agcacaatta tctggtggac agaaacagcg tgtcgcaatt gctcgggcat tatcaatgaa
     2581 tccagatgta ttgctattcg acgaacctac ttcggcgtta gaccctgaaa tggttggcga
     2641 agtgcttaat acgatcaaag acttagcaca tactggcttg acaatgctta tcgtgacgca
     2701 tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt tttatggata aaggtgtgat
     2761 tgcagaagaa ggtacaccag aagatatctt tgtccatccg aaagaagaac gtacaaaaga
     2821 attcctttca cgtattttaa atgcttagga tcaatagctg attgggacaa agagctgcgg
     2881 tgtttaccgc agttcttttt tatgaattac tcataaaagg taaatttctg aa
//
LOCUS       DAYYHX010000245         2902 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000245 DAYYHX010000000
VERSION     DAYYHX010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.248"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..555
                     /locus_tag="ACHA6Y_002661"
     CDS             <1..555
                     /locus_tag="ACHA6Y_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFM8988715.1"
                     /translation="EIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGV
                     IHALNTMRLDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNV
                     LTAKDERVCRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEY
                     DRLFGDLLDEFAHDSFGINLKRRK"
     gene            557..874
                     /locus_tag="ACHA6Y_002662"
     CDS             557..874
                     /locus_tag="ACHA6Y_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988716.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            1001..1624
                     /locus_tag="ACHA6Y_002663"
     CDS             1001..1624
                     /locus_tag="ACHA6Y_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFM8988717.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            1639..2529
                     /locus_tag="ACHA6Y_002664"
     CDS             1639..2529
                     /locus_tag="ACHA6Y_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFM8988718.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            2552..2767
                     /locus_tag="ACHA6Y_002665"
     CDS             2552..2767
                     /locus_tag="ACHA6Y_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988719.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
BASE COUNT         1089 a          397 c          592 g          824 t
ORIGIN      
        1 gaaattaaac attatttgaa ctatccatgg tcaggaaaga cattttcaag aagactttgg
       61 ggaaatattt catcgcttga acagaaactc tctaatgcta ttgtaaaaag tgttgctagt
      121 ggagaaggcg ttatacacgc attaaacacc atgagattag actcagaaat ttgtgacatg
      181 tttaagttag aagaatcaaa gtataacaaa gcgatagaaa atctcgttcg aacggagtat
      241 gcaaaatttg cacaagatgg tattgaaaaa tcatatttag aaacaggtat tgaggaatac
      301 aacgtattga ctgcaaaaga tgagagagtt tgccggattt gtggaggaaa ggcaagtaag
      361 aatccctata aactgaaaga tgctgtcata ggtgaaaatc gagcaccttt ccatagtcgt
      421 tgtagatgta cggatgttcc taatttacca aaattaggaa aggatattga tgaagaatat
      481 gaccgtttat ttggcgattt attagatgag tttgcacatg attcttttgg aattaatttg
      541 aaacggagga agtagaatga aagatttttt tgaagcagta ctaacaatta atgtaaatgc
      601 tgatattgca gaagcctaca aaacagctat tgagtctgag aaccatccta atggcttgag
      661 agaccattgg aatggcaatt atgcctacgt ggttattggc gatcaaactg ttaattatca
      721 agataatact ccagttgata agaataccgt taatttaacg attcaattat tatctcattc
      781 attaccaaat ttaaaagaaa cagttgattg gtatgaaaag atgggatgta ttgttgttag
      841 aactgactac aaagaaggaa agtctagtaa ttaggctttt ttattttgtc cgaaatgacg
      901 ttaaactagc gcaatactgg gcttggttga atggtggggc gcaactatta aaactcaaag
      961 caatgcgggg cgtgaaaacg aatcgtgggg cgaaaggaga atgagcatga aacacaaatc
     1021 attaatgcca atgaatttgc aatactttgc cgaaggtgac gatcaaaagt tttcttttga
     1081 tgacttcaaa tcttttgtgg aatccaatga agaagctcaa aaatttattc agtcacaatc
     1141 tcaaagtgtt gccgacaaac aattggaagc ttggaaacaa aataacttag ataaaattaa
     1201 acaggatacc atcaaggaat atgaggaatc taagaaaaac aagtcacctg agcaaattca
     1261 actggaaaaa ctacaagctg aatttgaagc agaaaaagcg ttgcgtgtga caagtgataa
     1321 taaagcattt gttgcagaac aaattgctgg attagaacta gatggagagt taaaagagtc
     1381 tatttctcaa tttatgctaa ataatcttgt tagttcggat acagatttca ctaagaatgc
     1441 tgttgaaggt tttacaagtg ttttgaatgc aattaatgag aaacatgcag atgcattaaa
     1501 agaactacaa atgaagtctg catttggtgg aactcaacaa tcgaataacc aggttcagca
     1561 gaacaatgaa acattaacaa atccagaaga acaattagga caaattcttc aacaatttaa
     1621 ctaggagagt gaaaaattat gaaaaaaaca tctttaaata atttagagta tttggatatt
     1681 tcaccagcga ttaatgctat gcaagtacca aatacacctt ttttaagcta tttacttggt
     1741 gctggaaaaa cagagccagc aaactcgaca gaaattaaat ggcgtgaata tgatatcaac
     1801 aacgatgatt cttctgaaaa acttgagggc ggagaatatc cagatgctga atcaggtcga
     1861 acttggttta acaactatac tgaaattttt agaaaatcaa cctctgtatc tggtacatta
     1921 gatgctatta atgtgaatgg agtcggaaat gaattaacta atcaagtagc actacgtggt
     1981 atggaaatga aaattgattt gaaccgaaaa ttgattactg gtgtaaaagc tgatgaaaat
     2041 ggttctaaag gtcgtcgaat gaatgggatt ttgaacttga tcaattcagc aaataaggca
     2101 gaaacagcca ctgcgggtgc agtaacaaga aaagatatcg atactttatt taaattgatg
     2161 tatgaaaaag gttatatggg agaaaaacta tgcttgattt ctccagatat gcaggagtta
     2221 atgactgatg agttagatgg aaaatcaaca aaaattgttc agttcgggga aagagtaact
     2281 tttggattgc aaattggaaa tatcgtgtct aattacggta caggtattgc tctactagaa
     2341 ccatcattgc caaaaggaac aattgccgca atcgatacta attatgtgaa actacgtcca
     2401 ttacgtgaat ggagagcaga agaacttgca aaaacaacgg attctagacg tatcggtctt
     2461 gtaggtgaat actctcttga atacaatgct tcaaactctg gggcaatttt aaatttaaag
     2521 tctgaataaa agggagttag tactcccttt tttgatagga ggaattacag tggtaaaaaa
     2581 agatgaaact aaaaaagatg aagtcgtgaa atatagagta ggtaaaacta aaaattttgt
     2641 tgggtttgtt catcctaaaa ctcgtagatt tatcacagca gattcaaata acgaatttat
     2701 catttctata gatgataaag aagcaattgc aattttggaa gatgcaattg atgttaatga
     2761 aatttaggaa gtgatctgat ggatgaatcg ctaaaaacgg aaatcattga gtctacaaaa
     2821 gaagattttc cagatttgag tgaagaacgc ataactaatt tattagaaat aattttgcta
     2881 gaaattgaat catacaacac tt
//
LOCUS       DAYYHX010000246         2901 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000246 DAYYHX010000000
VERSION     DAYYHX010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.249"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..204)
                     /locus_tag="ACHA6Y_002666"
     CDS             complement(<1..204)
                     /locus_tag="ACHA6Y_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988720.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLC"
     gene            complement(930..2270)
                     /gene="glnA"
                     /locus_tag="ACHA6Y_002667"
     CDS             complement(930..2270)
                     /gene="glnA"
                     /locus_tag="ACHA6Y_002667"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM8988721.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(2298..2690)
                     /locus_tag="ACHA6Y_002668"
     CDS             complement(2298..2690)
                     /locus_tag="ACHA6Y_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8988722.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          885 a          618 c          465 g          933 t
ORIGIN      
        1 acataataag cttgatataa acatagctaa cgttaaacct aagcaaatca gaaaatcgtt
       61 actgaaaaat tttaagtgaa ataatagata aaaaaataaa acaaacatta tgatagataa
      121 tatcccacct aacaaagaac tactttttcc tacttgcagc aacagtttat atagcttatc
      181 gtagaaacaa tctttttctt tcacccttat ttacctcctt tacgtaaaaa acgtagtcca
      241 cagttctcgg atttttggca tatcttttct attaaataat atcaaaaaca aaacaattcc
      301 actaattttt tcaaagatga cctttttttc aacaaaagtg ccttgtaggg attcattgaa
      361 ggtaacgaat cacataagta aaaaataagg agatgcgaca aaagtcgtgc ctccttattt
      421 ctaaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccatcaaaca
      481 tgagaagctg tttttgaaat tatttcttgt gattcggagt taaatggtag gctcacaacc
      541 tctggataaa cggtattcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat
      601 gtagaaagcc attttcacaa aatttttctt aatactcgaa actaaacgtc tttttcaaaa
      661 cctcttggta aacggtgttc aaaagcttgc atctgcggta ataagttcag ccaattcaaa
      721 aacaagaaaa gctgtttttg cttggctatt cttattactt gttgcagggc agctttttca
      781 caacctctcc actaaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc
      841 aaaatatgag gaactatttt tccgttagtt tctctaatac tcaggtcaaa acgctgattt
      901 cacagcctct aattcacggt attcctgttt taataaagtt ctagatattg ttctctttcc
      961 cactcggaaa cagtttgtct gaatgccgcc cattcgagac gttttgcttc aacaaaattt
     1021 gagaagatat gttgtcctaa tgcgtcaatc attacattat ccttacgcaa ttctttgata
     1081 gcattatgga tagttgaagg taagtcctgg atatgtgcag cttctctttc ttcctcatcc
     1141 attacataaa tgttacggtc aacagctgct ggcggtgtga tctgattacg gataccgtct
     1201 agtcctgctt gcaacaatac tgccattgcc aagtatgggt ttgcagatgg atcaactgac
     1261 cgcaattcca aacgtgtgga taaaccgcga gattctggta cacgtactaa tggcgaacga
     1321 ttacggccac tccatgcgac ataaacaggc gcttcataac ctggaacgag acgtttataa
     1381 gaattgactg ttggattgca aacggctgtg taggcacgag catgtttcag caaaccacct
     1441 aagaaatgat aagctgtttc acttagttcc aattcaccgt ctttatcata gaaaacattt
     1501 ccttcatcat tgaacaaaga catattgcaa tgcataccag aaccattgat cccaaataac
     1561 ggtttcggca tgaaagtcgc gtgaagtcca tgcttgcgtg caatcgtttt gacaacgagc
     1621 ttgaaggttt ggatattatc acaagcatct accacatctg catatttaaa gtcaatttca
     1681 tgctgtcctg gcgctacttc atgatgtgaa gcttccactt caaagcctag actttctaat
     1741 tccaacacga tgtcacggcg gcaattttca cctaaatctg ttggtgcaaa atcaaaataa
     1801 ccacctttat cattcaaggt cgttgtgatc ccaccgtctt cgtctaattt aaataagaag
     1861 aattctggtt caggtcctaa attgaaagag gtaaaaccaa ggtcgcgcat atctttcaat
     1921 gctcgtttca aattcccgcg aggatcgccg gcaaatggtg tcccatccgg attatagata
     1981 tcacaaatta aacgagcaac ttttccatga gcactttccc acgggaagat catccaagtg
     2041 gagagatctg gatacaaata catatcgctt tcttcaattc gaacaaatcc ttcaatcgaa
     2101 gaaccgtcaa acatcatttt attttctaat actttgtcga tctggctcac tggaacttct
     2161 acatttttga tcgtacctaa gatgtctgtg aacatcaatc gtaaaaatcg tacgttttct
     2221 tcctcaatga ttcgtttgat ttcttcacct gttatctgtt tctttaccat ggtatccctc
     2281 ttcctttttt aatttattca gtcaaagaat tgtctttgca atccttgcga ctgataatga
     2341 cctgttttgc ttaacccacc ttgcgccaaa atctcatcac gaaaaatttg tcgaacgtca
     2401 tgatctgtca acggcttctt gttttttgct tcttgttttt tcgcctgtgc catttcgtat
     2461 acacgtttga tcccagccat attcaatccc tctgaaagat agtctttaat ctcaagcaac
     2521 atatcaatat cattcagcga atacatacgt cggtttcctt cgcttcgttc cggatgaact
     2581 aaatcttgtt cttcataata gcgaatttgc cgagcagaca aatcagtcag cttcataact
     2641 gtaccaattg gaaatacgga catagatcgg cgcaattcct tttctcccat cagacgtccc
     2701 ctttcgtttt ataagttggc taaagtgtac caattaaaaa aaatattgtc aactgcatat
     2761 gttaggtttt ctaacatgag atctctattt tttataaaat aacaaaaaaa taagaacaca
     2821 aaaaaggtat ctagttgatt ttcgtaccag atacctttta ttttttatta tactgtacta
     2881 tcctttatta gtgtttttat t
//
LOCUS       DAYYHX010000247         2899 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000247 DAYYHX010000000
VERSION     DAYYHX010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.250"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..227
                     /locus_tag="ACHA6Y_002669"
     CDS             <1..227
                     /locus_tag="ACHA6Y_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8988723.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..1620
                     /locus_tag="ACHA6Y_002670"
                     /pseudo
     CDS             227..1620
                     /locus_tag="ACHA6Y_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294791.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1635..1841
                     /locus_tag="ACHA6Y_002671"
     CDS             1635..1841
                     /locus_tag="ACHA6Y_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988724.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1862..2458
                     /locus_tag="ACHA6Y_002672"
     CDS             1862..2458
                     /locus_tag="ACHA6Y_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HFM8988725.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
BASE COUNT         1054 a          396 c          574 g          875 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca tttaaataaa actgagttta acatatataa gttgattgaa
     1201 tccgttattg aaaatttcgc cagcaaaatt gagtcaaaaa accttcatgt tgaaatccaa
     1261 ggggataaca tcaatatatc tgcagataaa gataaaatga accaagttgt tatcaatctt
     1321 ttgaataatg ctattaaatt tacgcaaaag gagggaacca ttaagttttc tataagtaaa
     1381 aataaagacc atgttctttt aattgttgaa gacaacggca tcggtataga aaaagatcag
     1441 caacttcata tttttgatag attttatatg gctgacccct ccagaagtag agctttaggg
     1501 gggcaaggta tcggattagc tattgtaaag agcgttattg aagcgcataa aggtagtata
     1561 actgtaaaaa gtaaattagg tctaggaact aaatttgtta taaagttacc atttcaataa
     1621 aggaggagag ttatttgttt caaaaacaga taaaaattgc taaactagta tgtgaatgtt
     1681 gggcaaaaaa aatatttttt caactgattt cgatagatag tgtcgaaaaa gttcatttag
     1741 atatcttatc aaattctgta gtaatctttt ctaagaagga cttatcaata gctacaatta
     1801 aaaaagaagt ttttggatta caaatttatg agatgaccta aatgtgatta attaaaggag
     1861 gatgaagaca gtgaaaaaga tgacaaaatt agtatttgca agtcttggaa ttattgtatt
     1921 tttaggagcg tgtagcaatc aaagtgaatc gaataattct aatagcacaa acgaggaatc
     1981 gacttctaca gcgtctagtg aaatgaatag tatggaggga atgaatcacg aaggtatggt
     2041 tccttcttca atgaaagatg ctgcgaaccc taaattcccg gtgggaagta atgttatact
     2101 gctaggcgat catatggaag ggatgaaagg tgcaaatgct caagttgtag gggcctttga
     2161 tactacaatg tatgaagtaa gctatgagcc aaaaactggg ggaccaatgg ttaacaatca
     2221 tcgatgggta gtacaagaag agttaaaaga tactgagact gtagcaaatg agggcgatac
     2281 tgttatcttg aatgccgatc acatggatgg gatgatggga gctgaagcaa aagtagataa
     2341 atctattaca ggaactgttt acgttgttaa ttacactcca accgatggtc aagaagaagt
     2401 taagaatcat atgtgggtaa ctgaagatga gatggaatat gacgaaaata atgagtaatt
     2461 ttatatacta aaccaacaag ctggtacatg gtcttcgtat tctcaacatg aggaatatga
     2521 gctctttgtt aataaggact gatgagttgt gcaaaatcaa atctgggtca gaacgaacct
     2581 cattctggct cagatttttg gctcttcgac atttagtgct ggtagagaga caactgaaaa
     2641 tttagtcata accagttagg cagagtgctt aactggtttt tgttcgcatt tattattctt
     2701 ttgatattgg ctgcttgccc ttcaatttta tttggtttct taatcaactc ttcatataac
     2761 cccaaatcat atattaatga ggtgcctcct attcctataa atctatctct tctttaatag
     2821 tatgagaaaa gtctactctt gtagatttaa attgtcaaca aaaaatttac agatgtaaat
     2881 gataagttga atagagaaa
//
LOCUS       DAYYHX010000248         2869 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000248 DAYYHX010000000
VERSION     DAYYHX010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.251"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(23..277)
                     /locus_tag="ACHA6Y_002673"
     CDS             complement(23..277)
                     /locus_tag="ACHA6Y_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988726.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(441..764)
                     /locus_tag="ACHA6Y_002674"
     CDS             complement(441..764)
                     /locus_tag="ACHA6Y_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988727.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            951..1024
                     /locus_tag="ACHA6Y_002675"
     tRNA            951..1024
                     /locus_tag="ACHA6Y_002675"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:985..987,aa:Ile,seq:cat)
     gene            complement(1409..1906)
                     /locus_tag="ACHA6Y_002676"
     CDS             complement(1409..1906)
                     /locus_tag="ACHA6Y_002676"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM8988728.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1946..2755)
                     /locus_tag="ACHA6Y_002677"
     CDS             complement(1946..2755)
                     /locus_tag="ACHA6Y_002677"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8988729.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT          956 a          508 c          478 g          927 t
ORIGIN      
        1 ctacacatgc aatcaatagt actcaatgtt ttttatttaa acgtatcaaa taaagaatga
       61 cgatcaataa agacaaaacc agtcctgata attcttctat aaaaacacta tcgggttcaa
      121 agtagttgta attaaaaact ttccacataa agtagatagc cggaagagtc aaaatagcta
      181 aataaaaccg ttctttgttc caattcataa gataaacaat acttgcgacc agtgcgaccg
      241 caggcaccca aaacaaaact ctcaccaacg taatcatgta acggactcct caacgattca
      301 tataataaaa gataacatgt tattgcatac atcacaaatg aaatgctagg aaaataaaag
      361 aagaatagat caatgaaaaa tgaaaaagca gaacagtttt ctgctgataa gcaggaactg
      421 ttccgctgaa tgtgtcttat ctatcgagaa aaaatcaatg gattgatatc taagtaattc
      481 aatacatata aaaagaggac gatcgcattc actaaaaagg aaagaatgat gatcccatag
      541 cgaatggaaa catatttaac aaatactttt gtttgaatac gggcatatct ttttccttta
      601 tagctgagca tcatgacaag aagcaaaagg gtaatcacac cttgaaaaat cattccaata
      661 aacagaattc ctgaggtttg ataatttaat tggagaaaat aagctgctgc cgcaactaaa
      721 tcgaataaaa tcgcaataaa tggcaaaata atcactccta acattgagaa taaataaaag
      781 aataacattt tttctaaaat aagcaaataa aaattaaagt aacaaaatag ttatgacaca
      841 agtaaaagaa ataagaataa caatattatt ttacttatat tttgaaactt gtgagagttt
      901 gtcattcgtg ctatagttga aaagtaacaa aaataaattt tatttatgat gtacctttag
      961 ctcagttggt tagagcagac ggctcataac cgtccggtcg taggttcgag tcctacaagg
     1021 tacataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc caagtaaaaa
     1081 cagcttctta tgtttttact tggctgttct tattactgca gatgcaagct tttgaatacc
     1141 gtttaaggaa gaggttgtga aatcagcgtt ttgacctgag tattagctta acaaacggaa
     1201 aaatagttcc tcatattttg ccgtttgtta agctaatacc gagggtcagc tgattgaaca
     1261 ccatttaaga aagaggtcgt gagcctgccg tttcactctg tgtcataaaa aagccaatct
     1321 actttgatag aaaaagcacg taggactaaa acagttctgc gtgctttttt tattgtgtgt
     1381 taaatatcgt aaatgaagat agatattttt atgattcgac gatagagacg ttgaaatctt
     1441 tcaacagctc taaagcggat tctccgtgtt ttctactgga gactaacatt ggatcaacaa
     1501 aaaagtctgc ttcaggaaaa gcactctgca ggcctatcag attagagagt acacacaagt
     1561 ttgtttcaat cccgactact tcgatctctt tttgttcttc tttgctttca aataaagttt
     1621 cctggatttc agctaaagtt tcaggtggaa tcgtaaagta atacttttta atcactcgtt
     1681 cattttcttg tggagaaaga agagggatca attgcaaatc ttctcgttct tcttctttgt
     1741 ccttcagttc aactggtata tcccgtgtga agagaatata ttcattattt tggcgtgctt
     1801 tagctaatct ttgagcaata cgttctgcca attgatctga ctctggaata taaaattcgc
     1861 tgtttggatc aagaatatga ttttgcatat caataataac taacattttc tttcctccta
     1921 cattcatatc gtcattttat tctttttaat agaagttttt taaaatataa gcaacaccag
     1981 cttcttcatt cgtcagtgtt acttggtttg caatttcttt tacttgtcgc tgtgcattcc
     2041 ccatcgcaat actcaacccg ctgtaattca acatagcaat gtcgttttct tgatcgccaa
     2101 aagccatgat gtcactcgat tcgattccaa ggtaatccgc cagaaaaaca agtgaatgag
     2161 ctttagtgat acctgtaggc gtgatttcta agaaatgcgg atcagagaat actgattgga
     2221 taccatctgc ttgcagatct ctttgtagct tctgtagttt ttcttcactc ttatctgtta
     2281 atgctaattt aagatagcta agagaaggat cttccaacat atttaaaatt tgatcaaaag
     2341 aatggttctg taaattgaaa tgttcataat aagggtcatc tttgactaga tttgattcat
     2401 cgaatgtcag ctgatcatct tgggtttctc ggctagcaat gatggatatt ttcgcttctt
     2461 gagcatagaa gaaggctttt ctcagtgtat cgctatctat ttttgaacgt ctgagaactt
     2521 tcgccatttt tccatccttc atttcagcaa tcagtgaacc attggaagaa atcaaatagc
     2581 caccgattcc taattcggaa aaaatatctt tagcagaaat gctgttcctt ccggtagcga
     2641 cggttacgat tccgccattt tcatcaaatt tccgaagtgc ttggatattt tcttctggta
     2701 tctttccatt tcgatcaagc aaggtaccgt ctaaatcaat cgctgctagt ttcattttat
     2761 cttcctactt tctttttaca tatctatttt cctgttacct taagattaaa tgagaattta
     2821 aaaaaatgaa actaacttgc tttgtcatta tgaatttttt caaaaaaca
//
LOCUS       DAYYHX010000249         2864 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000249 DAYYHX010000000
VERSION     DAYYHX010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.252"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1885)
                     /locus_tag="ACHA6Y_002678"
                     /pseudo
     CDS             complement(1..1885)
                     /locus_tag="ACHA6Y_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            2065..2730
                     /locus_tag="ACHA6Y_002679"
     CDS             2065..2730
                     /locus_tag="ACHA6Y_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8988730.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
BASE COUNT         1005 a          501 c          464 g          894 t
ORIGIN      
        1 taaaaagata tttaggctct ctttctataa gaaaaaggaa gattacactt gtcgttcccc
       61 atttgatgag gttcatcatg aagaaatctt tatacgtttc aaagagcgaa taaccacttg
      121 aacgaaagat agcgattttc cgacaattgt agacaaaata tatgcataac atgacatagc
      181 tcaacaagaa gaaagaaaag aaagtgagta aaaatatctg tatcaagtaa tgaaatgatc
      241 tgcttaacac tttgacagct atctcttcag tctgagtata cggaaatgat aactgcaagt
      301 tatcacgtaa ctgactttct tcaatcagct caaataacct ttctttcgac aaagagccta
      361 tcttgatctt taacggtgga tagatttctc cgctgagaat atttctttcc caatacgtac
      421 tgtttttcaa tgtcagtatt tcaacaaccg gataatgttc gatccagggg atagaaggaa
      481 taaatgtata aatgggttga ttgtttttta taaatagaaa tttcggttct ttttcactga
      541 tttcttgata atattctgtt atttgcgcca ggtcattttt taaatctgta tgtctatatc
      601 gttcagggat caatataaca gggtgcgttt ctttttcggt tatttggatc aaccttccag
      661 agacatcaat caaaggaaat ttcttaagat aattcagatt tatttttatc cctctgccaa
      721 actgttcatt ttcttcattc aagtaatctg atacattgac taatatactt ccgtttttat
      781 tcaaagtttc atataattcc tgatcggctg ctgatgcaaa acgattggaa taaataaaat
      841 ccacgtggtt tttcccgatg gttactggat aaaatacatg atagtcctca ccagcgattt
      901 cttttggtgt ttcatgggaa acagctaact gtgaagaaga aaaaacgata gttgctaatt
      961 cgacattggc aacggtcaaa agaagagaaa acaacaatcc aaatgtaaaa agaatgtatt
     1021 ggatatagtt gaacgcttct cttaacgaag cgattttttc aactaataaa agagaaatca
     1081 aagagatacc ctatattccg ctgaacaaga aaacgtacat ctggaacaaa taaagaaatt
     1141 gctccttata atacataaaa ataaaaccac caatgattcc tgcaattatg aatgtaataa
     1201 tgattttctt tttccccaat aaacaccaca atacttgatg taatgaaaag ccattattca
     1261 acaataaact gatttcttta ttcatcaata agaaaaagga tgataacaaa aggaataaaa
     1321 gaatcaatcc aatagttaaa tacactcgat aattatatac gtctgtactt gaaataaccg
     1381 aatacgaata gctattttca aagtcttcaa aaatattagt ggcataatca gtgtgaaatg
     1441 cttgattgaa tcgttccaca aatagtaaaa tgaaccgttc ataggcttct ttttctctcg
     1501 tcatcaagaa aaattcacct tcaaaatctt ttattttgat cgattcttca atggacgtta
     1561 cgcgaaagga ggtattagaa tagggtacct tctcgtgaga agatttgaca tccccttgat
     1621 aatacaatgt catttcaagc tttggctgta tcttgaagtt ccaagatgta tctacttcat
     1681 tggattcatt tactgttttt ttcatataag aaagctcagt ttctgatgag acattttcaa
     1741 ataccatgat tagctgctca tattcttctt gcccaataaa atgttgtggc aatcgaatcg
     1801 gagtataagt atctgtttga tggaaaaatt catttgtccg atagagtacc tgtttattta
     1861 taataaccaa ggcaaaacca ctcatgatac cggtcactat gattgtgagt aaaactgtga
     1921 actttttttt gattatcacc actctccctt acttaaatga ttctttgata aaatcatacc
     1981 gtttttcatc aaaaaaacgt atctatcaaa tgtataggtc atgtagaata aatctatata
     2041 ctgtagaatc gaaaggagaa gaagatgaac atcggagaaa aaatacgaac ttaccgcaga
     2101 aattgtaagc ttacgcaaaa agaattaggc gaaaaaatcg gtgtttccga taaaacaatc
     2161 tctagttggg aaaattctcg tacaatgcca gaccttgaaa tgctgtcact tttacatcag
     2221 actttaggtc tgccgattga ttttccttct attgaatcag ctaccgaatc aatagaacca
     2281 acattgccgg atgaacctct tgttatcaag aaatcttatt catcaaaaca acccttctca
     2341 ctcaaaaaga cactatcttt ctttctgctc gtcttttgtt tggtctatct ttttcttatc
     2401 aattatcaat ataaaaatga aacggttgat cgattcaatc cttttttgaa accacaaatc
     2461 ggctatacca cactccctgt aggatacgtg gaaaattcca tcgaacattg ggtatgtaat
     2521 gatatctttg ttcatggtac atggctgtct tttcacgctg gagaggtaga aaaaggtaag
     2581 aatcatgctg taatcaaaca caaaggaaaa cacgtcatga acgttcgcct gctcacttgg
     2641 gaagccattc cctcagatta ccaagaaatc ctcatccaag actatgacga tgccttaaaa
     2701 agaagccctc aggagccaga taggcattaa gtaggagatg aacgtagaga tagaaaaaat
     2761 caatgcacat gatcttatca aaaaaaaaca ggggtacaaa ttaattgaaa aagctttgag
     2821 atgaaagttc ctaaataaaa aagttcagaa tgataggtaa aaaa
//
LOCUS       DAYYHX010000250         2839 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000250 DAYYHX010000000
VERSION     DAYYHX010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.253"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            379..711
                     /locus_tag="ACHA6Y_002680"
     CDS             379..711
                     /locus_tag="ACHA6Y_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8988731.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            621..824
                     /locus_tag="ACHA6Y_002681"
     CDS             621..824
                     /locus_tag="ACHA6Y_002681"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8988732.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            855..1001
                     /locus_tag="ACHA6Y_002682"
     CDS             855..1001
                     /locus_tag="ACHA6Y_002682"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988733.1"
                     /translation="MTDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            1439..2779
                     /locus_tag="ACHA6Y_002683"
     CDS             1439..2779
                     /locus_tag="ACHA6Y_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8988734.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT          947 a          568 c          529 g          795 t
ORIGIN      
        1 atgattttaa ttattaacgt tttttaattc gtttttagct taaaaataac ttttgtcaca
       61 gtctcactcc taaaattact ttatttacgt acgaagtggg caggaggtcg aatccagaac
      121 tgggccatgg tacttcaacg aggacttctc ctctagaatt gaaaagcatg cgatttacct
      181 tccgtaacga cggtagattt attcaactat gtgtccaatc aaaatttgtc tgatttcaaa
      241 aatagtcaag agaattttgg ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt
      301 gacaaaccct gatactaagt tgggctatga tacacttttc ttgactatca gttacgttac
      361 cggaaggaat gaactctatt gaaaaaaaca tatgatcgta aatttaaact aaagatttca
      421 caggatatcc ttgagaaaaa agtaactacc aagaaaattg ctgaagaata caatatctct
      481 cgtccaacaa tttcccgatg ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct
      541 ggacaaggaa aaagattacc tgataaagca gatttctaca ttcttgaaca ggaaaataaa
      601 cgactaaaag aggaagacga gtgttctacg aacacaaaga tcgatacgga tctccaggga
      661 tcactcaaaa actttacgat gaaggaatcg aaatcaataa acgtgttgta gctgttctta
      721 tgcgtgaaat gaatttatgc acaagaggat ttcatcgacg caaatcttct catggtagag
      781 aaaaaacaat caaagaaatg gtaaaaaaaa tgttgaatcg ataatttgaa cagaaccaaa
      841 ttgatgctgt ttgggtgacg gatattactt acattccatg taatgacggt cgcctctatt
      901 tgtccaccta tattgactta gcaactcgta ttctacgtta ctatatggtg gattcacaca
      961 tgaaaaaaga gattgtcgtc cgtccattac aaatttatta aggagaattg cctacggtaa
     1021 ttcattccga tagaggtagc cagcatcggt cttttattta tcaagagctg ttgaccgacc
     1081 accacatcac acattcaatg agccaaactg ggactcctgt tgacaatgca gtaattgaag
     1141 cgtttcatcg atcaatcaaa tgagaattaa tcgactccaa caaatataaa agtaaaatag
     1201 agatgaaggt gttaatccaa gattatttag aagactacta cccaaacaaa agaatccata
     1261 caaaatttat gatgactcct tgacaatttg aagcgaagaa aataagggat ctataatatc
     1321 tagtgttggt gacgccttct gttgccaata ctttttcttt tttctaataa acgcaaacag
     1381 acaccttgaa atcctttaga ataaagtcga cgaaaaccat tcaaaggagt gtttcaagat
     1441 gtcctattca gagtttacca aagagttact agatattcta gacctaaatc ttacctttca
     1501 tgaagatgcc tttcgcaaag agcgcattaa tgacgaaaca tgttttgttt ttgatggcac
     1561 attgacctat caaccagagg aatgcttcca ttgccactat caaaataagc aaacgatcat
     1621 taaatggggc tggaaaaaag tatccatctt attgaatgat gtcagtaact acaagaccat
     1681 tgtgcggatt aacaaacagc gcttcaaatg taaacactgt ggaaagactt ttttagcaga
     1741 agattcagtt agcgatcgtc gctgctccat tgcccgcaga gtcaaacaag ccattcttga
     1801 gctattaagt gagcctattt ctatgtcttt gattgcacgt atgaagcaca tttctccgac
     1861 cactgtcatt cgaatacttc gcagccttcg gcctaaaacc gtttctttga accagccgtt
     1921 accagaagtg gtgtgtttcg atgaattcaa atcggtgaaa aacgtctcgg gagccatgag
     1981 ctttgtcatg atggacggca agacccatca actgattgat attgtggaaa atcggcaact
     2041 caatccttta cgcgactatt tcttgcgcta ccctagaaaa gtccgtgaac aagtacgtct
     2101 agtggtttca gatttctata caccctatcg cacattggtg aaagagtgtt ttccgaatgc
     2161 ccgaatcgtc gccgatcgct ttcatattag ccaacacatc ggtcgggcct ttaccaacca
     2221 ccgaattcaa gtaatgaaga cctttaaaaa aggggatcgt cggtcgaagc acttaaagaa
     2281 atactggaga ctacttcaaa agaatgcgtg ggaattgaat ggtcagcatc gctactggcg
     2341 tccgtcattt cgagatcatc tgacagaagc ggagatagtc gatcggctat tatattatga
     2401 tgactcctta aagcgaggct acgaagtcta ccaggttttt ctttccgcta tcagaagaca
     2461 agatgttcct gaattcgtcg cgctcctaaa agaagactac aaggagttgc cagagcacta
     2521 tcaaccagtg ttcacgacct tcaagaaata tcgaacagaa atcaaacgag cgttgcgagt
     2581 tccctattcc aacggcccta tagaatgttt gaacaaccat atcaaagtac taaaacgaat
     2641 tgcctatggc tttagaaact ttcaaaacta ccgagaacga atctttttat atcgaggaaa
     2701 atatttcaag aaaactaaga acactaccca actgactaaa gccagaacaa caacacggct
     2761 agacaagata gctgtctagc cgtgtgagta tctcgattat tctattgaat ttcttcacca
     2821 acactcattg acgaagagc
//
LOCUS       DAYYHX010000251         2830 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000251 DAYYHX010000000
VERSION     DAYYHX010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.254"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..348)
                     /locus_tag="ACHA6Y_002684"
     CDS             complement(<1..348)
                     /locus_tag="ACHA6Y_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HFM8988735.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKG"
     gene            498..980
                     /locus_tag="ACHA6Y_002685"
     CDS             498..980
                     /locus_tag="ACHA6Y_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM8988736.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1017..2027
                     /locus_tag="ACHA6Y_002686"
     CDS             1017..2027
                     /locus_tag="ACHA6Y_002686"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM8988737.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2142..2828)
                     /locus_tag="ACHA6Y_002687"
     CDS             complement(2142..2828)
                     /locus_tag="ACHA6Y_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988738.1"
                     /translation="MVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAEKSSTQKSAS
                     TETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLAGYSDLQIEY
                     ARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVMLTGKYSYQGV
                     LVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAIPTGNPNDVK
                     QLIDVTVIDN"
BASE COUNT          942 a          458 c          578 g          852 t
ORIGIN      
        1 acctttttta tcttcaatat taaatgaaat caattgttca atcaattccc agttgattac
       61 acttttccaa ggaactttga tgacgttatc cgtatatacg tagcctgctt cttggatatt
      121 tttttcaagg gcacgaatcg ctgctttttc cggtgcgata gaaaaatgat ttttagcaaa
      181 actaaatgca ataataaagg taccatggtc agtaaacata ggttggttcc attttacagt
      241 tgtgtctagc tggggatacg tttctgttat cttagcaaaa aggtctttga tcttttgttg
      301 atgttctttt tctttgattt gattgatgat cggctgaaat ttttccattc taaaacgcct
      361 ccattaagtg gtttgattta ttttaccaga aaaacaatag ttatgtataa aaaacgaaca
      421 aaacacaagt aatgttgtaa ccgctatatt tatatgataa gatgtctcaa ataataaaat
      481 agaaaagaga cataaaaatg attgacttgg caaagaaatt cttacacgaa aatatatatt
      541 ttgagttgta tcatcatcga gccatttata caaatgaaga tgctttggta ataaagaaag
      601 aacaaggatt tacaggtaca gagaccaaaa gcttattctt aaaagataaa aaagataata
      661 actacgtata tctgactttt actacgaaac aaacggattt caaagcatta gggaagatcg
      721 tcggtaaaag gctttctatt gtttcatcag aacgcatgga agaacaaacg ggacaaaagc
      781 agggcgctgt ttctccgttt ggttatgaaa aacagattcc gattattatt gatgaagagc
      841 tcttatcaaa tgaaaaactt gtttttgcac caggtagacc agatcaaacg atggtcatcc
      901 aaacggttga cttaaacaaa atcatccagc tacttgagat gccgatattt ttgatgccgg
      961 caaacgaaaa aaacaattaa ccaagtaaaa tagtgcatac ttggaggtga agcaacatga
     1021 taagttatcc attattagaa agaaatgcaa cgattggggt tactgcacca tcctcaggcg
     1081 tatcagagga acaccattcg ttactcgaat cagctttaaa gagaatggaa gaaaaatctt
     1141 tccaagtagt tactggaaag acgctttgga cgcaagataa ggaaaaatca ggtccagcaa
     1201 gaaaacgagc agatgaattc aatcaaatga tgcaagatga aaatatccaa atgatctttc
     1261 cgacttgggg aggagaatta ttgattgaga ttctcgattt tgtcgatttt gatgctctac
     1321 aaccaaaatg gatagtaggt tattcagata ttagtacgtt gctacttgct gtcacattaa
     1381 aaacagggat agccactgca catggaacta accttattga tttaagagga gagtacttgg
     1441 atgaaacgac agcaatgtgg gaaagtgtac tgaaaacaag agaaggagaa tcgattctac
     1501 agcaatcttc tgaaaagtat caaaaagtgt ggccccatga gcattcatca tcttctgttt
     1561 tccatctcac tgaaccaaca aattggcaat cagtcagcgg agatgcagta aggatgaagg
     1621 gacgattatt aggtggttgt attgatacga tcaggcattt aatcggtact ccttatggag
     1681 atgtccaatc ttttaaaaga aagtataatg atggaaagcc gatcttgtgg tttctagaaa
     1741 actgtgagct atcgtcagtt gatttaagaa gatcacttgt tcagatgaaa ctggcaggct
     1801 ggtttgatgg cagctcgggg atcatgttcg gtagaagtgc ggcagaggat ttgttagatt
     1861 ataaaatgaa ggatgtatac gaagatctgt caaaggaatt aaaactaccg atcttgtatg
     1921 atattgattg tggtcatgta ccgccacaac tgacattgat taatggtgcc tatgcagaag
     1981 tagcggtaga agatagaaaa gcgactgttt tgcaaacgtt tatttaaaaa attttttcct
     2041 gccttggagt ctatcaaaga ccaataaatc taaatagaaa agaaagtgaa agaagctgac
     2101 aaaatgctgt cagcttcttt ttgtataaca tattagaaag tctaattatc tataactgtc
     2161 acatcgatta actgtttcac gtcattggga tttcctgtag gaatagctac tacagatgca
     2221 ttatcaatga tttcttgagt tgtttctcta atgaacgctg gataagatgt ttgatctgca
     2281 ggcatttgcc aatgtgaagg aactggataa ctagtgattg tcccgtcatg gttacttgaa
     2341 tacactaaaa ctccctggta actgtatttc ccagtgagca taaccacatc tttcggatag
     2401 actgcactcc ctgcatcata aggggcgata ggtgttcctg caggataatg aataacatgt
     2461 aattcgaaat cacccgatgt atctctaaaa gtattacctc taatagctag ccaaacacgt
     2521 gcgtactcaa tttgtagatc agaatatcct gctaacggat ctattgactg agaagactgt
     2581 gaggaggttg tgtctattgt tgaagaagaa atatttgtct caactgtact actagatgta
     2641 gaagaagtat tgggctctga gactttactg cttgttgaag atgtattcgt ttcagtagaa
     2701 gctgatttct gagtacttga tttttcagct gaactggttt gtctgattga acttttgtct
     2761 gtggtcgcag atggatcact attttcttta ctaggagacg tacatcctac aaggaataaa
     2821 gagaccaaca
//
LOCUS       DAYYHX010000252         2773 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000252 DAYYHX010000000
VERSION     DAYYHX010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.255"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..1169)
                     /locus_tag="ACHA6Y_002688"
     CDS             complement(75..1169)
                     /locus_tag="ACHA6Y_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM8988739.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1358..2119
                     /locus_tag="ACHA6Y_002689"
     CDS             1358..2119
                     /locus_tag="ACHA6Y_002689"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8988740.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="ACHA6Y_002690"
     CDS             complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="ACHA6Y_002690"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8988741.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          880 a          551 c          467 g          875 t
ORIGIN      
        1 ctataaaaaa acaaaataaa aaagcagaaa taactggcta tttctagcgg attatttctg
       61 caataactaa ttatctattt tttattttca attaatttaa agcttgacca tggtttcaaa
      121 aagtcaagga ggaataattc gtcatcggag attcttccta caacattgac acgtccgtcg
      181 tttttcatat ccttcaaggc gatttgtgtt tctcctttgt attgaccgta cccttcattg
      241 tcaatcagta catccccatg atgcatatct cgcgtattat gaggcgggaa atctttatct
      301 ttgtagtata cacgagtcat cgttgaacga aggatatatt ctgagcgatc ccctcgataa
      361 ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg taataccttc ttccgtatct
      421 actttcaatg ttggataatc cgcgttaaat gcttctgcca tttctttcaa ttcttcttca
      481 cttgcgtacg catttccaat agaaatatca tcaatcaatc ctgtaaggat taaatgcttc
      541 acttgtgtcg caatttcaag atctcggtgg tcttccagcg tgcacaagcc atcttgcgta
      601 ggccaagggc caaactcagc agattgcgaa ttgacgaatg ccatcgtatt caaattatat
      661 tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc ctaaaccaga gtatcgatga
      721 ggataaaaat tatgagaacc taataaatta tccgtgtttg gcgaataact catgatgttg
      781 tcaacgtaat tcgtgcccga actcatattg atctcgattt tgataccaaa aggattgcgc
      841 gtcatttttg cttcttctgc tcctgtgaat ccgatatcta agcggacccc gtaggcaccc
      901 atcttatgga aaaaagataa gtcatcgtaa gaaatctcca actgttcaaa taacgctgga
      961 ttgatatcaa ccattacttc catgcctagg ctattcgcgt aatcaactac ttctttaaat
     1021 tctgaaagaa ctttttcttt atcatcattg atctgcagca aactggtaaa aacccgttta
     1081 tacccatact tatgagctaa atccaagtac gctttgtcct tttcaaatgt tgaccgttct
     1141 ggataaatcg agattcctaa ttttcccata ttaccatttc ctcctgcatc ttttcgtttt
     1201 tgtatccact tacattatag caaacacaaa gtaaatggtc tacacctttt ctttttcttt
     1261 taaaataaat gataaattcc aataatttaa gtaggtattt tccctctatt ttgttatcat
     1321 taaagataac aaaatagtaa attggaaagg aacagttatg aagaaaaata aagttatttt
     1381 aagtattagt ttagcagcag cattgctgtt tgctgctggt tgttctgcaa acaacacgac
     1441 ttcttctagt tcgtctacag aaagttcaaa aagtacatct actgcggaaa gcacgactgc
     1501 cagtagcgtc gatttgaacg cattggaatt accacaattg acgaaagatg tcgccgaaaa
     1561 tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc aaaatcaaat tatttcccga
     1621 acacgcacca aaagcggttg aaaactttat gacacatgca aaagacggtt actacaacgg
     1681 actgactttc catcgcgtca tcaaagattt catgatccaa ggtggcgatc ctaaaggcga
     1741 tggcactgga ggggaaagca tttggggaga aggatttgaa acagagatca acaaccacct
     1801 ttataatata agaggtgcgt tatctatggc tcgctcacaa gatcctaaca gtaacggcag
     1861 ccagttcttt atcgttcaaa atacagatga tatgcatgat ggtcttttaa aagacgacta
     1921 tcctcaagca atcatcgacg cttataaaaa cggcggttat ccttcactag atggccatta
     1981 tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg gatgctattg caaatatcga
     2041 aacagatagt tcagacaaac caaaagaaga agtaaaaatc gataaaatca atatactgca
     2101 agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg catagatgac aacgtatgct
     2161 aggattcagg cgtcttcttt ttacataaaa agcaagtaag cttccttttg ttttgctata
     2221 tcaggtttta tagtcaagca ttttggttga cgatgaacgt gaactcgcac tcacagactt
     2281 ttttatcatc tacatatgct gcagataacg ctgtaccgac tgatcccttc attttggtga
     2341 tttcaaaatg acatttcagc acatctcctg gaacaacttt ttgacggaat ttggccttat
     2401 tgatcccacc gatataagct ctttttccta gaaactcttc tgattttagg ataagtatcg
     2461 aacctgcttg tgccagtgtt tctagaatca acgcacccgg catcactgga ttgtcaggaa
     2521 agtgaccttg gaaaaaatct tcattgattg ttacattttt tgtcgcaacg atcttttttc
     2581 ctggttctaa agtatctaca taatcaatat aacaaatagg ataacgatta ggaatcaatt
     2641 ccattacttc agtagcagat aatacgtaag acataattaa actccttctt tcaggcaatt
     2701 gccattttca ttactttgac tatcaaagta tcatattcct aaatatttag caatcttttt
     2761 ttaatttaag aaa
//
LOCUS       DAYYHX010000253         2763 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000253 DAYYHX010000000
VERSION     DAYYHX010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.256"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..552
                     /locus_tag="ACHA6Y_002691"
     CDS             106..552
                     /locus_tag="ACHA6Y_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8988742.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            825..2177
                     /locus_tag="ACHA6Y_002692"
     CDS             825..2177
                     /locus_tag="ACHA6Y_002692"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM8988743.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            2348..2635
                     /locus_tag="ACHA6Y_002693"
     CDS             2348..2635
                     /locus_tag="ACHA6Y_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8988744.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          974 a          451 c          545 g          793 t
ORIGIN      
        1 tttttcatta aaatacaata aaattgaatt ttcttttcat gttaagtgga ttttaaaatg
       61 taaaaatagt atgataagac gagaataaca acgtggggtg tttacatgga aaataagtta
      121 gtcgaagagg ttatcgaaaa gttaaaaaaa gagaacattc gaatcacacc tcaacggtac
      181 gcggttttag aatatttgat tgaacatcat tcccatccaa cggctgatga gatttataaa
      241 gcacttgaac atcggtttcc aaatatgagt gttgcgactg tttacaataa ccttcgctta
      301 tttacttcga ttggatttgt tcaagaaatg aagtacggcg atgcttctag ccgatttgat
      361 ttcagttcaa agcgtcatta tcatgtaatt tgtgaaaact gcggtaagat tgttgatttt
      421 cattacccag ggcttgaaga tatagaaatg gctgcaagca gactgactga ttttgagatt
      481 aatgaacatc ggttggaatt gtatggactc tgtccggatt gtaaagagtc agcaaaaaag
      541 gcgcaaatgt aaaaacagtt tctacaagtg ttatacctaa aaaaagctag ctgtgtaaaa
      601 aaaacgctag cttttttatt tttttgtctt ttattcacaa tcttagatat aaaagtgaat
      661 attattaaaa taagaatctt gtagatattg ggttcttatg cttgaaaagg aatgaaacta
      721 caaacaaacc tttgtgaatt cacaagcatt aaactgttgt atccgtttta tttctgttat
      781 agtagttttg tcgaaggaca aaatgttaat cggagggacc ataaatgaaa gtagtagtaa
      841 ttggctgtac tcatgcaggt actgcagctg taaaaagtat tttaaaaaat catccagaag
      901 cagaagtgac agtatatgaa agaaatgaca atatttcttt cttatcttgc ggtatcgcac
      961 tttatgtcgg cggagtagta aaggacccag caggcttgtt ctactcaaat cctgaagaat
     1021 tatcttcatt aggagcaaca gttaaaatgg aacacgatgt aacagacgtc gatacagaaa
     1081 ataaaacagt aacggcaaaa gatctgaaaa caggagaaac agaaacggtt tcttatgata
     1141 aattagtgat gacaactggt tcatggccta ttatcccacc gattagtgga atcgatgcga
     1201 aaaatgtact cctatgcaaa aactacaatc aagcaaatga aattattgca caagcgaaag
     1261 aagcaaaacg tgtagtaatt gttggtggtg gatatatcgg tattgaatta gtagaagctt
     1321 ttgtcgaatc tggaaaacaa gtgacattga ttgatggatt agatcgcatc ttgaataaat
     1381 atctagataa accattcaca gatatcttag aaaaagaatt gacagaccgc ggtgttactc
     1441 ttgcgctagg cgaaaatgtt actgaattta tcacagatga agaaggaaaa gtgaaacaag
     1501 tagctacacc aacagataca tttgatacag atatggtcat tttatgtgtg ggcttccgtc
     1561 caaatacaaa actgatcgaa ggaaaagtag atacactgcc aaacggggca atcaaagtga
     1621 atgaatacat gcagacaagt aatccagaca tttttgcagc tggagactct gcagtagtca
     1681 actacaatcc aagtggcacg caaaactata ttcctttggc aacaaatgcc gttcgtcaag
     1741 gtttgcttgt gggaaataac ttgacagatc ataaaatggc gtatcgtggt actcaaggaa
     1801 cttcaggact ttacctattt ggctggacga tcggttcgac aggtgtgacc aaagaaagcg
     1861 caactttgaa cgatctagaa gtccaagcga ctgtatttga agataattat cgtccagagt
     1921 tcatgccaac tactgaaaaa gtaatgatgg agctagtcta tgaaaaagga acgaatcgta
     1981 tcgtaggtgc tcagttcatg tccaaatacg acattacaca atcagcaaat accatgtctt
     2041 tagctgttca aaataaaatg actgtagaag atctcgcact ttcagatttc ttcttccaac
     2101 cacattttga ccgcccatgg aattacttga acctacttgc acaagcagcg ctaggcgaaa
     2161 tcgaaactgc acaataaaga aaaagggttg tgacacaatt ttgtcacgac cctctgtctt
     2221 tttttgtgaa aagtacaatt agttgtcttt cttcgaattt tttgtcacaa taagcagcaa
     2281 agataaagct gttacataaa agaacaagca gctttatttg attttgaaaa gaaaggtgag
     2341 aaagaagatg aataaagaat attttgtcca acaagatgca tttttagaag attataaaag
     2401 tgcttcgaag aaattaacga tcaatattga tgagttggca gatcaagtac tctctcagtt
     2461 gaatgaaacg caagaggaat attacacgat gccagcaaat gacgccaaag ataatgtttc
     2521 tcatcgtttt cctttcagta gacgcatcta ctcagatgaa aatgacggca cattgaatac
     2581 agagtacaca tatgaaggtc agccatatgt gctagaaact gatgatgatc tttaaagaca
     2641 gtttttgaaa aggccgctca tccttaagtg atgacaaaaa atcatcacaa ttaccgatga
     2701 ctgtctcttt actatctttt aatcagaaaa aggggttatg acaagctttg tcaacccctt
     2761 ttt
//
LOCUS       DAYYHX010000254         2752 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000254 DAYYHX010000000
VERSION     DAYYHX010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.257"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..468
                     /locus_tag="ACHA6Y_002694"
     CDS             46..468
                     /locus_tag="ACHA6Y_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988745.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            488..2077
                     /gene="mobP2"
                     /locus_tag="ACHA6Y_002695"
     CDS             488..2077
                     /gene="mobP2"
                     /locus_tag="ACHA6Y_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFM8988746.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            2089..2424
                     /locus_tag="ACHA6Y_002696"
     CDS             2089..2424
                     /locus_tag="ACHA6Y_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988747.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            2565..>2752
                     /locus_tag="ACHA6Y_002697"
     CDS             2565..>2752
                     /locus_tag="ACHA6Y_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988748.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         1091 a          415 c          560 g          686 t
ORIGIN      
        1 ttatcgaact tttaattgaa caaagtagag gaggagaagt ttaacatggc caaagttata
       61 gacaagcata tttattttta tgaaacacat atagatgttc tagatgaaat tattgcaact
      121 catgcagagg ttaaaaacta tagtgaagcc gttcggtttc tatgtatgaa ttatgattca
      181 gaagtggaga aaaaagtaaa ttccaatcaa gcaaagttga atgcaatggg aaaagaaatt
      241 tctattttaa ccgaaatggt ttcagagttt gcggatctcc atttgaaaga tacagggatt
      301 ttaattggta caaattcaac tgtttaccaa gaagcaaaag agcgtgtgga aggcaagata
      361 aaagcaaatc aaacgaagaa atattctcct aagataaaga aaacaataag cgaatcagtg
      421 actacaaaaa gtgaagtatt tgatccaaca aggttcttaa aacgataaaa attagaagga
      481 gaatgtgatg agtaaatcac ctgcagtcat tctcacttgt caatttacct tgcccaataa
      541 aaaatctttt tcaacataca ttgattatat gacaagagaa aaagcgctgg aagaaaagga
      601 acgacgtacg cccgaggaag aacaggaact gcgtctaatc aagaatgctt tagaggattt
      661 ttatattcct gaaggagaaa ctttttcgga gagtaagacg ggtaaagaat tcagcgaaac
      721 gtcgaatgaa gcgcaatcgt tgatgaaaca tggaatcaat tttgacaagt tagaagaaca
      781 agattttacc aaatatcttt cttatatgac acgtcactat gcgttagaac aaaagaaagt
      841 actgacgcat agtgaacaaa aagaatacca acgcttgaac caagcaataa aaaaatatca
      901 aacagagcca cacaaaaaag cttcaggaaa tcttcctgga gttttttcta tggcttcaga
      961 cgaagtgaaa ggaagtgatc tgaaggagat caagcaaacc ttccaaagag gacaacaaaa
     1021 aggctcaatc atttatcaag atgttgtctc tcatgatcat tcgtatcttg aaaaaatagg
     1081 attgtacgat cccaaaacag atactttaga tgaagaaggt ttaaaagcag ctggaagaaa
     1141 aatgatggag acattgttcg agaaagagca actgaatgaa acaggctact ggatggcaac
     1201 gattcaccga aataccaaac atatccatat tcattttgca gttgttgaaa aaaaaaatac
     1261 gcgtaaacca tacactgaga ttgaaaatgg tgtgagttat atcgtaccga aagggaagag
     1321 aaggcaagca acattagacg aaatgaagac taattatgta catgagttag aacgtttttc
     1381 ttatgaaaaa aatcgaatgg gtatggagaa acgaaattta ttgacacgga aaagtgattt
     1441 gcgtaacacc ttaaccaaag tagtgaaaga gccagtacgt tatgatcgac acgcaatgag
     1501 cttattaact gaagtatata aaaacttacc taaaaagcgt tcggaatggc attacgggga
     1561 tgaaaatcga accaaacttt caccatacac aagagagaaa ctcgatgagt taacccaata
     1621 tgtgttaaca aaagaacctg gatatcaaga atatatacag atttcaaaag cattgaaaga
     1681 agaagcaaaa agtctttatg gggaaagcaa gagagaatca aaggatgcag aaaaaaatgc
     1741 tctctttgat ttaaaaaagc gcacaggaaa tgccattcta acagaactga agaaacagga
     1801 tagtcaaata aaacctttaa tagaaaggat ggatcagatg caatctattt acaatgaacg
     1861 atcctttcga acacttaaac agttgccaac gaatgaatac aaatatagtt acgaagaatt
     1921 catgaagcgc aggaaacaat tccaacaaag agttgtcacg caacggagtt tgcgtcaatt
     1981 aaatcgaacg attaatgccc atgaagaaaa ataccacgca agtaaagcat acgaagagca
     2041 acaacgaaga attcaaatgg aacaagaaag acagtaggag gaatagcaat gaaacaatat
     2101 ttagatcaat ggaaagttat agaaggatca ttaagagaag aacgaatcga acaacttccc
     2161 gattgtttag aaaaagagca tttgtttcag ataagagaaa tgttgataaa cgaacagttt
     2221 gatccaaatc aatttttagt cgttgagtac ccagctactg gtgtgtactg ttgtaatcat
     2281 gtgaagggtg aaaaatattt tatcattcaa gaatatgagg gcaagttagc gccttattat
     2341 actacttggg aaatgaatga agaggggatc aataacttcc cgtgtaaaag cattgaagag
     2401 tctatttcac tcacagaatg ttagttgtta agtgaataaa agaagtaaaa gagtcctgaa
     2461 cgtgattgtc caggactctt ttttttcact gaattgtttt ccgccaattc attgagtggt
     2521 gcgatttccg tctacattat atcaaaagaa aggaggaaga agaaatggct tattcaacga
     2581 ttggagttgt tgttgaaaaa agcagagaca atcttgtttt tgtgacagaa attcaaacag
     2641 gcagagcttt tgtagtcaca gacaaagcgg caaaagcata tcaaagtggc gatattttaa
     2701 cgttaaatct gactacgaag acgttcgtcg atgcggcaga agattatcca tt
//
LOCUS       DAYYHX010000255         2725 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000255 DAYYHX010000000
VERSION     DAYYHX010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.258"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..388
                     /locus_tag="ACHA6Y_002698"
     CDS             2..388
                     /locus_tag="ACHA6Y_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988749.1"
                     /translation="MEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYF
                     ANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEY
                     NYIVKAKVSKRFIFKPKLEIYSVVIE"
     gene            713..1330
                     /locus_tag="ACHA6Y_002699"
     CDS             713..1330
                     /locus_tag="ACHA6Y_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8988750.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1371..1670
                     /locus_tag="ACHA6Y_002700"
     CDS             1371..1670
                     /locus_tag="ACHA6Y_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFM8988751.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1704..1871
                     /locus_tag="ACHA6Y_002701"
     CDS             1704..1871
                     /locus_tag="ACHA6Y_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8988752.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1916..2521)
                     /locus_tag="ACHA6Y_002702"
     CDS             complement(1916..2521)
                     /locus_tag="ACHA6Y_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8988753.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          957 a          408 c          498 g          862 t
ORIGIN      
        1 tatggaagaa atagaatgga ataatctgac tcaaaataat aaaaataatt ctggatgtgg
       61 aattatcttt atgtttgtta tagttttggg aatagtagta cttttaataa ctgtcaagat
      121 agtagattac tttgcgaata ctcctgaaga acaaatacag agaaaacttc ctgagtttga
      181 aagagtacca ccttatgatc caagagataa ttcatttcaa gtaaaaaaat taaaagagtc
      241 tccaaatgaa atgtggtttg aagttacagt tgaatcaagc gacttcaaag aaaaaaagaa
      301 agaatacaat tatattgtaa aggcaaaagt atcaaaaaga tttattttta aacctaaatt
      361 agaaatatat agtgttgtaa tagaataata gtgttaaaaa aattgtaaaa gttaaagaag
      421 tcaaattctg atgtgaaaaa taaaatatac catggaagac ataaaagaaa aactggataa
      481 ctagtttatt taatggcaga ccgttaaggc ctgccatttt tatgtatttt agcggaataa
      541 atataatcat gcgggagtgt aaacttactg tttcaaaaag tatacttttt gacatgcctt
      601 gattttcttt gtttatgtat ggcataataa ccacataata caatttgaaa cgtttctgat
      661 ttaatctgat tgttttgaaa caattttagt gaaaaaaggg tagataaaaa cgatgagaaa
      721 atttttttat atccgggtca gtagttatga tcaatcgatc gaacgacaac tgatttccgc
      781 gaaggaatta ggaatccctg aagaatatat atttgtagaa aaagccagcg gcaaagattt
      841 taaacgccct gaatatcaat taatgaaacg gatgtttcga aaaggagatt tactctatct
      901 acaatcatta gatagactag gcagaaataa acaaatgatt ttagaagaat ggcgagaatt
      961 aactaaggaa aagcagattg atatttctgt gttagacatg cctctgttag atactgctag
     1021 ttacaagcaa ctagatgggt tagagacgct tatctctgac ttaattctgc aattactcag
     1081 ctatatggcc gaagacgagc ggaaacgaat taatagccgg caaagagaag gcgtagaagc
     1141 tgcgaaacaa aagggtgtaa aattcggcag aaagaaaatt gctttacctc ctgaatttcc
     1201 tgcgatctat tctgactgga aagcaggaaa gataactgca gtctatgcta tggatcaatt
     1261 gggcatgaaa cgaaatacct tttatcgacg ggtgaaagaa tatgaacata ccttgcacca
     1321 cttttcttga tagatataac agctattaga cgggaaggga gagtttgaaa atggacttaa
     1381 cgaatcaaag ggtcgttttt actggaacgc tgcagcaaat gacacgaacc caagccatcg
     1441 gtttggttca ctctttaaac ggccattgtc aatcaagtgt tacttcaaaa acaaatttcc
     1501 tggtttgtgg tcagtattct aagtcgttgt ttgatgattc tctctataca aaaaaggaac
     1561 aaaccgcgcg ggacttgatt gcgttaggtc atgagattac cattttatca gaagtcgaat
     1621 tttatgcgtt aattagtcaa cagttgaaag aatggcaacg gtatttataa tcgttagtaa
     1681 aatttacaag gaggaaaatt tttatgaagc gaaaatctta tgatcgttgg ggcattgaaa
     1741 taactagttt taatcgaaat attgcttttc acgtggagtg tgactgtggg gaattagcaa
     1801 aaagtgttta tcaaaaaaat tacttttact gtcctaaatg ccatcgccga tacggggtac
     1861 atggaacata gaaacgaaat agtgcggtag aaaagcgaat gaatgtagta tatttctaca
     1921 aatcattcgc tttctttatt ttataaatag ttggtcgaga gatcccgttt tcttttgcaa
     1981 tctctgcaac tggaatgcct tgctttaaca tgtccaccac ggttttaaaa actaagcgtt
     2041 tttgcggatc tgttgagtta atagaatact cgattggtcg tcccttatat cggccggctt
     2101 tttgagccaa taatactcct tgccgctgac gttcacgaat tttctcacgc tcgttttgtg
     2161 caatataact tagcagtgat aaaaacatat cgaacatagc ggtatccagg agttcatttc
     2221 cagtattaaa atctaaaaac tgtgcatcta atatttttaa cgctactttt ttctgtttca
     2281 taaaagcaac tgtatttttt atatcttcat aatctcttcc taaacgatct agtgatgtaa
     2341 cgatgacgca atcaccttca cggatataat ttagtaattt ttgaaactca ggacgctcag
     2401 tatctttacc actggctttt tcttggaata atttatcaat gttaagtcct tccatcattg
     2461 ccaattgtcg atccaaattt tgatcttgtg aagatactcg aatatagcca atttttccca
     2521 taaaaaactc ctttttaaaa gtgatataat ttacatgtaa aatttacttg tagttttact
     2581 gctatcatag catttttaaa aataaatttt atgtagactt aaaatttact tcatggaggg
     2641 tttaaatgga tgaaatcaaa agtaacaaaa aagcatttgg catttattta ggagaaccta
     2701 gtacagaaca attaaaccaa ttttt
//
LOCUS       DAYYHX010000256         2705 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000256 DAYYHX010000000
VERSION     DAYYHX010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.259"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..519
                     /locus_tag="ACHA6Y_002703"
     CDS             175..519
                     /locus_tag="ACHA6Y_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM8988754.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="ACHA6Y_002704"
     CDS             520..939
                     /locus_tag="ACHA6Y_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM8988755.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="ACHA6Y_002705"
     CDS             complement(1031..1381)
                     /locus_tag="ACHA6Y_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988756.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2678)
                     /locus_tag="ACHA6Y_002706"
     CDS             complement(1347..2678)
                     /locus_tag="ACHA6Y_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM8988757.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
BASE COUNT         1002 a          489 c          523 g          691 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaaacac ctgtaaaacc aagataaaaa tactttcttt tctgtgaaac gattcgtttt
     2641 tgaggatgaa tataaataac gtcagtttcg ttttccatta cacaccttct ttttcatttt
     2701 cttta
//
LOCUS       DAYYHX010000257         2705 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000257 DAYYHX010000000
VERSION     DAYYHX010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.260"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..201
                     /locus_tag="ACHA6Y_002707"
     CDS             <1..201
                     /locus_tag="ACHA6Y_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8988758.1"
                     /translation="IVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVL
                     SVVFASSLMLIKKPKEILSKIS"
     gene            211..867
                     /locus_tag="ACHA6Y_002708"
     CDS             211..867
                     /locus_tag="ACHA6Y_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8988759.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            867..2243
                     /locus_tag="ACHA6Y_002709"
     CDS             867..2243
                     /locus_tag="ACHA6Y_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8988760.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            2373..>2705
                     /locus_tag="ACHA6Y_002710"
     CDS             2373..>2705
                     /locus_tag="ACHA6Y_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8988761.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1067 a          320 c          481 g          837 t
ORIGIN      
        1 attgtagatg gattcattaa ctcagaggat tcaatgattt ccggtggaag tttaataaat
       61 aatagtagtt ttatgtcaaa tataacaaca cttggacaaa gttatttaat attaataagt
      121 attattgttt tatcagttgt atttgcttct tcattaatgt taatcaagaa gcctaaagaa
      181 atcttatcaa aaataagtta ggagaaaata atggatatat tagaaataaa gaatgtagca
      241 tatagttatg caaattctaa agaaaaagtt ttgtcaggag taaatcaaaa atttgaactt
      301 gggaagtttt atgcgatagt aggaaagtca ggaacaggaa aatctacact tctttcctta
      361 cttgctggac ttgataaacc tcaaacagga aaaatattgt ttaagaatga agatatacaa
      421 aataaaggat atagtaatca tagaaaaaat aatatatctt tagtatttca aaactataat
      481 ttaatagatt atttatcacc aattgaaaat attagattag taaataaatc agcagatgaa
      541 agtatcttgt ttgaactagg tttagataaa aaacaaataa aaagaaatgt tatgaaatta
      601 tctggtggac agcaacaaag agtagctatt gctagagcat tggtatcaga tgctccaata
      661 atactagctg atgagcctac tggtaatcta gacagtgtta ctgctggaga aataattaat
      721 atattaaaga cattagctaa agatagaaat aaatgtgtaa tagttgtaac acatagtaag
      781 gaagtagcag attctgcgga tatcatttta gaactaagtg gtaaaaagtt gaaaaaagta
      841 aataaaatga atttggaggt tgaataatga taaaaaatgc atttgcttat gtaactagaa
      901 aaagtttaaa atcattaatt atcatattag ttattttatc tatgtcaact ttaagtctca
      961 ttagtttatc tattaaagat gctacggaca gagcttcaaa agaaacattt gctaatataa
     1021 caaatagttt ttctatggag ataaatagac aagtaaatcc aggaacacct agaggtgggg
     1081 gaaatgtaaa aggtgaagat attaaaaaga tatctcaaac agatagtata gactcctatg
     1141 taaaaagaat aaacagtgtt gcagatttag ttgattatga tattattgaa actcaagaga
     1201 ctcttgcgaa tcaatcacct gaaagagcaa agaattttaa aagaacagtt atgttaactg
     1261 gggttaacga ctcaacaaaa gaaacaaaat ttgtatcaga agcatataaa ttagtagaag
     1321 gaaagcattt agaaaatgaa gataaaaata aaatcttaat gcataaagat ttagctgaaa
     1381 aaaataacct taaagttgga gataagataa aaataaaatc taatttattt gatgctgata
     1441 atgagaaagg tgcagatgaa acagtagaag tagaaattaa aggtctattc gatgggcata
     1501 atagcggtgg agtaagtgca gcacaagaac tatacgaaaa tacattaatc actgatattc
     1561 atacagctgc taaagtttat ggtaataccg aagatacagc tgtataccaa gatgcaacat
     1621 tctttgtaaa aggtgataag aatcttgata gtgtaataaa agatcttggg aaattagata
     1681 taaattggag agaatataat ttgattaaaa gttcatcaaa ttaccctgca ttacaacaat
     1741 ctatatcagg tatttactca atttcaaata aattattttt tggctcatta atatttgcag
     1801 gagttgtagt ttcattatta ttattcttat ggatgaatgc tagaaagaaa gaaatagcag
     1861 tattactatc attaggtata tcaaaattag aaatttttgg tcaattccta attgagatgg
     1921 tatttatatc aatccccgca ttcgttggtt cctatttctt agctcaatat acagctgaca
     1981 agcttgggaa taatatattg aataaggtta ctggtgatat agctaaacaa atagctaaac
     2041 aatctgcatc tagccaatta ggtggtgggg cagaagctga aggatttaac aagacattat
     2101 caagtttaga tatcaatgta ttacctaaat tcatgattta tgtagttata tttatgagtt
     2161 tagtacttct tgtatcattg attatttctt cattcaatat attaagaagg aatccaaaag
     2221 aattattaat tgacaataat taaaattttc ggtgcttttt agcaccaaaa atgagagtgt
     2281 aatataaacc gtgtaagtaa ccatcaatcc taggcgggtg gttgcttcac ggtttttatt
     2341 tttctaaaat aagatcaaga ggtgattaca cgatggctag aaagaaaaga aatcctgatg
     2401 ctgagaagtt agctgaatcc attttgaata cttatcaacc cgaatctgta gaggatatgc
     2461 aagatgtttt aaaagatgtg tttggtccct tatttgaaaa aatgcttcaa ggagaattga
     2521 ataaccattt agggtatgac gcccattcta aagagccaaa gaaacatgac aatcgacgaa
     2581 acggttatgg caataagacg cttaaaacga gctttggtaa agtaaacatt gatgttccca
     2641 gagaccggga ggcttctttt gaacctgagt tgattcctaa tagaaaacga gatgtctccg
     2701 acatc
//
LOCUS       DAYYHX010000258         2686 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000258 DAYYHX010000000
VERSION     DAYYHX010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.261"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1184
                     /locus_tag="ACHA6Y_002711"
     CDS             <1..1184
                     /locus_tag="ACHA6Y_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM8988762.1"
                     /translation="ASIAIKVIFLSVIVFGSVMYLLNITKLWQDVYGLEKYIFLYYIS
                     YVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANIFFLLILKWGLIGFFIANILSQI
                     ISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATVVGWWINSTADRYVVSFIV
                     GVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDNETAKFYGNTFVMINLLMC
                     ITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIANVLNCASGLLGPILSAKKDSKSM
                     MWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSYVIYVVRKRAVGKDIFIKHYHIV
                     VESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINISYIKQLCYRGRNIISMIKSREHK
                     R"
     gene            1220..1936
                     /locus_tag="ACHA6Y_002712"
     CDS             1220..1936
                     /locus_tag="ACHA6Y_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8988763.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            1955..>2686
                     /locus_tag="ACHA6Y_002713"
     CDS             1955..>2686
                     /locus_tag="ACHA6Y_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFM8988764.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLN"
BASE COUNT          965 a          315 c          510 g          896 t
ORIGIN      
        1 tagcatctat agcgattaaa gttatatttt taagcgtgat agtctttgga tccgtaatgt
       61 atttgttaaa tattacaaaa ttatggcaag atgtttatgg attagaaaaa tatatttttt
      121 tgtattatat atcctatgta tggaatcaat ttctaataca atttgctaaa gggttagaaa
      181 aagttaaagc tatgggcata gcaggtatta tcagcacaat tgtaacaata attgccaata
      241 ttttcttctt gcttattctg aaatggggat taattggttt tttcattgca aatattttat
      301 cacagattat ttcagctact tatctgtcag tattagtgag aatatgggaa tatatcgatt
      361 ttgaggctaa agatcctcag ctaaagagag aaatgctaaa ttactcgatt ccattaattg
      421 caactgttgt tggatggtgg attaatagta cagctgatag gtatgtggtt tcctttatag
      481 taggtgtctc ggcgaatggc ttattgtctg tggcatataa aatacctcaa ataattaata
      541 ctttgcaggg aatatttata caggcctggc aaatttctgc aattaaagaa tatggagata
      601 atgagactgc aaaattttat ggaaatacat ttgttatgat taatttgtta atgtgcataa
      661 cctgttcaag tcttatcata ttaactagac cgttttcaca tattctttat gcgaaagact
      721 tttatgaagc gtggaaatat gttccatttt tattaatagc taatgtttta aattgtgcat
      781 caggtctgtt aggacctatc ttatcagcaa aaaaagattc taaatccatg atgtggtctg
      841 caatcatagg ggcattagtt aatgtgatta tgagtgtagt ccttgtttat aagatagggg
      901 ttcaaggtgc cacaatagcc actgttatat gttcatatgt aatatatgtt gttcgtaaac
      961 gagctgtggg aaaagatatt tttataaagc attatcatat tgttgtggaa tcttggggat
     1021 tattgtttgt tcaggcaatc atagaaatat attttaaaaa ttatgtaata gaaattataa
     1081 ttatgattat tttaattatt ataaatatca gttatattaa gcagttgtgt tatagaggaa
     1141 gaaacattat ttcaatgatt aagagtcgag agcacaaacg gtagacgatg ttatttttat
     1201 aaaaagatat aatttattaa tggtcgataa ttatgaaagg aaaaatattc ttaaaaaaaa
     1261 gagtatagga tgtcgtatga aaatagtcgc tatagtacca atgaagttaa ataacaaaag
     1321 attgccacaa aaaaacacga aagcttttac caatggaaaa ccattgtgtt attatatact
     1381 ttcaacactt ttaaaagtaa aacagattga taaagtgtat gtctattgta gtaacccaga
     1441 tattaaagaa tttataccag aaggtgtaac atttttaaag agatctagtt ccttagatca
     1501 ggatacaacg agtattacac aggtattagc cggtttcaca aaagaagtag aggctgatgt
     1561 atatgtaatg acacacacaa cagcaccatt tattagcgaa aaatctattg aaaaaggact
     1621 agatgccgtg ctgacaggag aatatgattc tgcatttgct gcaaaaaaat tgcaagattt
     1681 cttatggaaa gacggtgaac catttaatta tgaattggac aatattccta gaacacagga
     1741 tttgccgcct ttatacgaag aaactagtgg attttatatt tataatcgca atgtgatgac
     1801 cgagttgcat agaagaattg ggaaaaaacc ttgcatagtt gaagtaggtg agattgagtc
     1861 agttgatatt gatgaagccg aggattttat gattgctgat gcgatttata atcatcttat
     1921 tttacaaggt agttaagaca gggggaaggt taggatgaac aatgtacaga ttttagattg
     1981 cacacttaga gatgggggct attgtaacaa ttgggaattt ggatttgata atacaaaaaa
     2041 aattatttct ggacttatag aatcaggaat tgagattata gaatgtgggt ttctcacaaa
     2101 taaaacagta tacaataaaa atgttacaaa atatacgacc ttagaagaat tagcacagat
     2161 aattcctgaa aaaagagaag gaaaattatt tgtagcaatg atgaactatg gcgaatataa
     2221 tatagaggat cttccagatt atgatggttc atctattgat ggaattagga ttgcgtttca
     2281 taaaaaaaat tgcaatgaag cgttgaaatt atgtcgtgag ataaagaaaa aaggctactt
     2341 agtttttgtt caagctatgg tatctctcaa ttatacagat gaagagtttt tagcagtaat
     2401 aaaaaatgtg aatcagttta aaccatacgc tttttatatt gttgatagtt ttggaatgat
     2461 gaaaggaaaa gatttaactc gtctttttta catgattgag cataatctag aaaaagatat
     2521 acagataggt tttcactcac ataataacat gcagcttgca tattctaatg cacaaaaact
     2581 tataaccgta caaacaaatc gaagcctgat tattgattct tcggtgtacg gaatgggaag
     2641 aggagcaggt aatcttaaca cagaactatt tgtagaatat ttgaat
//
LOCUS       DAYYHX010000259         2645 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000259 DAYYHX010000000
VERSION     DAYYHX010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.262"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..427
                     /locus_tag="ACHA6Y_002714"
     CDS             <1..427
                     /locus_tag="ACHA6Y_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988765.1"
                     /translation="VNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQW
                     VNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGV
                     KGVADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            602..1435
                     /gene="msrA"
                     /locus_tag="ACHA6Y_002715"
     CDS             602..1435
                     /gene="msrA"
                     /locus_tag="ACHA6Y_002715"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8988766.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1515..2504
                     /locus_tag="ACHA6Y_002716"
     CDS             1515..2504
                     /locus_tag="ACHA6Y_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8988767.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT          968 a          422 c          519 g          736 t
ORIGIN      
        1 tgtaaacaaa gaaacagtag aggcgtatac cactaaagga atggattatt ttttatatga
       61 ttattctcct atcgcttcat tggagaataa ggagcatata ataaaacacg aagcaaagat
      121 tggcagtcaa tgggtaaacc ttccttataa aacaactggt agttctatca tatatagagt
      181 aactaatgag aagagccaag taatcacact tcctgtatat gcttatttag gaacgaatgt
      241 agaaataaat catcaacctg taaaggagca ggtgaatgct aatggattgg tacaaataag
      301 cggagtcaaa ggagttgctg atattaaagt aagcacaagt tatacagtaa cgactattat
      361 ttcttatctt gttacattaa ctgcatattt atgtttattt tttaaaatag ttttccataa
      421 aaggtaaatt aacttttcat ttataaaata aattgattaa aaaggagaat ctgtcgataa
      481 ggattctctc ttttttatac gaaaaatctg tagggggatg aagtaatcgc ttatttcctc
      541 actttatgat aaattacagc taaagtcact agcttgtctt cttcggaaag gaggagaatc
      601 tatggatgag gaacaaatat tcattcaaac actttataat ctgattttga atccgaatac
      661 acgagattgg gaacgaaaag tattgattca gacaaagaat gacataagag aaaatatatc
      721 cgttaaagaa cagttatcaa aactagaagc aacactacgt ccattagcta tccgaatgaa
      781 tctgacgcct gatgtaatgg acttttattt attgattacg gaaggatttg ataaggagca
      841 aaaatatgac ttttccaagc atgccatgca agatgctgac tatcaagaac gtgcagtatt
      901 tgcaggggga tgtttctggt gtatggtcga gccgtttgaa tcgaaaaaag ggatcctatc
      961 tgttttgtca ggatacacgg gcggacatgt tgaaaaacca aattatgatc aagtaagtgg
     1021 tggttacaca ggtcatgtgg aagctgtgga gatcatttat gacacaagag aaataagtta
     1081 tagcgagttg ctgacaatct attggcaaat tactgatccc acagatacat ttggacaatt
     1141 tcaagataga gggaagcaat atcgtccagt gattttttat caaggcgaac gacaaaaaga
     1201 attagcggaa caatcaaaac agaaactaga cagttcagga actttccacc aaccaatcgt
     1261 cacaaaaatc gaaccagccg gtacgttttg gccagcagaa aactatcatc agcaatttta
     1321 caaaaaacaa ccgaaacgtt ataaaaaaat ccagcaagca cgtaatcaat ttctgatcta
     1381 tcaacgagta aaaaacaaat ggcaaaaaaa tatacgaaaa aatcattttg attaactcct
     1441 tttctttaaa atgtagtaaa ctaaaaatag aaaaaagaaa gcgttttaaa aagatttgat
     1501 gaggaggaga aaaattgtat attgcagata gtgcacgtta tgaaaaaatg gaatatcgta
     1561 aatccggtca ttcaggtttg aaattaccga ttttatctct cggattatgg caaaattttg
     1621 gtgattatga tcctatccat aatcaaagag agattttaag aggcgcattc gacatgggaa
     1681 ttacccattt cgaccttgcg aataattatg gaggccctgc tggagctgca gagaaaaact
     1741 ttgggcgtat ttttagagaa gattttcaag cctaccgtga tgaactgatt atttctagca
     1801 aagcaggtta tcatatgtgg gaagggccat atggggaatg gggctctcgt aaatccatca
     1861 tttcaagttg tgaccaaagt ctccaaagaa tggggctaga ttatgttgac atcttctatc
     1921 atcatcgccc agaccctgat acacctttgg aagaaacagc agaagctttg atgcaacttg
     1981 taagacaagg aaaagcatta tatattggta tatccaacta taatggagaa gatacaaaaa
     2041 aaatgactga aattttaaaa agaaaggaag caccgttcat tattcatcag atgcgctaca
     2101 atatgttttc tcgtgcacta ctagaagacg atctatctcc agtattagaa gaagaagggc
     2161 taggggcaat cacgttcagt ccgcttgctc aagggttgct gacaaatcgt tatctacatg
     2221 gtattcctga agattctaga gcacatcgta aggaaatacc attcctttca gaagaacaag
     2281 ttggatcaac tctggaaaaa atcaaagcac tgcaaacaat cgccgtatca cgaggacaat
     2341 cattagcgca aatggccctt gcatggaatt tgcgacaaaa atctgttaca agtgtactgg
     2401 tcggagcaag tcggttaagt cagttacaag aaagtgttcg aatgatggat aatttagatt
     2461 tctcaccaga agaagaattg agaatcgacc aaattctaga atagaaacga tgtaatcaaa
     2521 ctttttatcc atatattaaa aaaaacaaaa gatgacatca agaatcgaat gaagatcgtc
     2581 tgttattgaa catcatattt tttgtcgttc aaaataaaaa tgatcgaagg ctaggagtga
     2641 atttt
//
LOCUS       DAYYHX010000260         2570 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000260 DAYYHX010000000
VERSION     DAYYHX010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.263"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..1014
                     /locus_tag="ACHA6Y_002717"
     CDS             7..1014
                     /locus_tag="ACHA6Y_002717"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM8988768.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDAIELMSGQEFIRIAKENNVVT
                     W"
     gene            1026..2030
                     /gene="neuB"
                     /locus_tag="ACHA6Y_002718"
     CDS             1026..2030
                     /gene="neuB"
                     /locus_tag="ACHA6Y_002718"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFM8988769.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2027..>2570
                     /gene="neuC"
                     /locus_tag="ACHA6Y_002719"
     CDS             2027..>2570
                     /gene="neuC"
                     /locus_tag="ACHA6Y_002719"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8988770.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILN"
BASE COUNT          940 a          331 c          512 g          787 t
ORIGIN      
        1 atataagtgt ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt
       61 gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt
      121 gaagaaaaac aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta
      181 attggtgata tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt
      241 tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta
      301 aaaacaaatg tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa
      361 cattttgtag gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca
      421 aagttattga gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca
      481 atattttctt gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt
      541 tttagtaaac aaataaaaaa caataaagat attactttga cagatgttga tatgacacga
      601 tttatgatgt caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa
      661 tttggagaaa catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca
      721 ttaataaaaa tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta
      781 ggtatcaggc caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc
      841 tcatatgaaa atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg
      901 tacaaagatt atgaaaaaat taaaatcact gcatactctt cagatgcaat agaattaatg
      961 agtggacagg aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt
     1021 ttatgatgaa atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc
     1081 tagatttagc tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat
     1141 ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga
     1201 aggataatct taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc
     1261 aatctgattt tattagaata aaagattatt gtatacaaaa taaaattgat tttttgtcta
     1321 ctccttttga tgaagaaagt gcagactttc taaaacaaat tggtgtacaa ggatttaaaa
     1381 ttggctcagg agatttaaca aatttacctt ttcttagaaa aatagatatg tatgggttgc
     1441 cagttttatt atctacaggt atggctgata ttcatgaggt cagtgaggct gtagattgtt
     1501 tcatagatag tccagttact attttacatt gtacttcaaa ttatccggct agttctgaag
     1561 atattaattt attggcgatt aacacattaa aagaaatgtt cgggataccc gttgggtact
     1621 ccgatcattc attgggatat gatgttggta tttgtgctgt tgcgatgggt gctaaggtaa
     1681 ttgagaaaca ttttaccttg gataaggaac tacctggtcc agatcataaa gcttctttag
     1741 atcctcagga atttgcagat tttgtaaagc atattagaaa cactgaaata tttttaggtg
     1801 atggtgttaa gcgagcaatg ccttctgaac ttagtacaaa agaagtatca agaaaaagcc
     1861 tagtggtcac tgagaattta aatgtgggag atattcttac agagaaatct cttacaataa
     1921 aacgtcctgg aaatggaata gctcctaagt ttttatatca atatattggc aaaaaagtta
     1981 aaagaccaat aagtgcagat agtgttctgc ttgaggaaga tgttgaatga aaaaaatact
     2041 tatagtaagt ggaacacgag ctgattatgg aatatataaa cctgttgctg agaatttact
     2101 acaagataat tgggatgtat catttgttgt ttctggaatg catttatccc ataaatatgg
     2161 atttactaaa gaattgatct tagctgacgg atttagaatt agtggagaag ttcaaagttt
     2221 gtttttagag aacaataagg gaaacatggc tcggtctgtg gcattagaga taaatggttt
     2281 cacaaatata tttgaacaaa ctagccctga ttttgtatta ttactagggg atagaggaga
     2341 aatgttggca gctgcgatag cttgtttgta tcttggaatc aaaacagcac atattcacgg
     2401 aggcgaagtg tctggtactg tagatgaatc aataagacat gccataacga aaatagcatc
     2461 tgttcatttt gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg gggaagattc
     2521 ttggagagtt tttcccgtcg gagctcctag aatagatact attttaaata
//
LOCUS       DAYYHX010000261         2570 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000261 DAYYHX010000000
VERSION     DAYYHX010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.264"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..1502
                     /locus_tag="ACHA6Y_002720"
     CDS             51..1502
                     /locus_tag="ACHA6Y_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8988771.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1851..2522
                     /locus_tag="ACHA6Y_002721"
     CDS             1851..2522
                     /locus_tag="ACHA6Y_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM8988772.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
BASE COUNT          965 a          364 c          508 g          733 t
ORIGIN      
        1 taatttttaa tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa atgaaaaaat
       61 ctttaaaact tgcatcagca gttatattaa tgtcccaact ctttatagca acacctttcc
      121 atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc gagacgactg
      181 aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact ttatcaagtg
      241 aaagtagtac gacttcaaat gctgagtctt caacaacttc atcaagtgaa agtagtacga
      301 cttcaaatgc cgagtcttca acaacttcgt caagtgaaag tagtacgact tcaaatgccg
      361 agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtgccgaa tcttcaacaa
      421 cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aagaccaaac catatagagt
      481 cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aaatttttcg acaccacagc
      541 aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat gaagaaattc
      601 attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag tctgcgagaa
      661 aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg atcttagaat
      721 ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt gggataaaag
      781 gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt aatggaacgc
      841 tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagt tttgaagatt
      901 atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca ggagcattaa
      961 aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg tatgcaacag
     1021 atacacaata ctatttgaag ctaaatgaat taattaaaac ctatgattta accaactatg
     1081 ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct attcataatt
     1141 attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga cttgatatgg
     1201 cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt aaagcagagt
     1261 ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca accgcgttat
     1321 atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa ggacaaataa
     1381 ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg gaaatttgca
     1441 aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta ttcggagact
     1501 aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta gacctttgat
     1561 caggatattg agactggaat tgatatctcg attacgatga gtttgacaaa taaaacaagt
     1621 ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg agcaggtttt
     1681 actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt cagtcttgta
     1741 ttataaatta gtcataaaat cagactaaga aaaattgaaa atattttttt ctgatttatt
     1801 aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca gtgaaggaaa
     1861 gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa aaatatcgtt
     1921 acctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta tcaggtagaa
     1981 ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat ttgacaaata
     2041 aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa ggaactgttg
     2101 tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag cgacttctaa
     2161 aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt gaaaataaaa
     2221 aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg aaaacaaaat
     2281 gggtttgtca atgtgagtgt ggcagtatgt gtgagataag ttatagtaac ttattttttg
     2341 gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt gttgaaggga
     2401 cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt gttaagggag
     2461 tttatcgttc taaagagaaa tggattgcgt atataacttt aaaaaaaaga ggtactattt
     2521 aggatcgtat gattctatta agagtgcagc tcaagttaga aaagatgctg
//
LOCUS       DAYYHX010000262         2517 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000262 DAYYHX010000000
VERSION     DAYYHX010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.265"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(525..1028)
                     /locus_tag="ACHA6Y_002722"
     CDS             complement(525..1028)
                     /locus_tag="ACHA6Y_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8988773.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1387..2412
                     /locus_tag="ACHA6Y_002723"
     CDS             1387..2412
                     /locus_tag="ACHA6Y_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8988774.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT          806 a          399 c          466 g          846 t
ORIGIN      
        1 aataacatat ctcattttac acttttgtta ttttatactt aaaaaataac gacacagaaa
       61 ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg
      121 ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa
      181 taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct ttttatagaa
      241 aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga ttgagatatt
      301 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
      361 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
      421 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
      481 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
      541 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
      601 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
      661 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
      721 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
      781 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
      841 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
      901 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aaatgatagc
      961 ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa ttttgataca
     1021 taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct gaaaaatcat
     1081 aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga agcttttttt
     1141 tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga aaagttgcta
     1201 tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc tccaagttgt
     1261 ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact aggtaatgct
     1321 gacaaacggg attggcattt catcttggtt cagctttttt tccaattact aggaaggagg
     1381 gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc ttttcgtatc
     1441 atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa tcatgatttt
     1501 ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca aaacataccg
     1561 atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt tgctttcgtt
     1621 ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc tttggttgct
     1681 ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg cttgtttttt
     1741 gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt accgatcgtc
     1801 tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat tacaaaatta
     1861 ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag atttcacgaa
     1921 gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct ttccgtttat
     1981 gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg tgcatcgatt
     2041 ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct gattggatcg
     2101 atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca gttgcagacc
     2161 atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag aaattatgca
     2221 gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat tcgtggagca
     2281 tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag tttcatcggt
     2341 ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt tgttcgtcaa
     2401 aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga tttttgtcac
     2461 aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa ttcacac
//
LOCUS       DAYYHX010000263         2485 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000263 DAYYHX010000000
VERSION     DAYYHX010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.266"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACHA6Y_002724"
     CDS             <1..435
                     /locus_tag="ACHA6Y_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8988775.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACHA6Y_002725"
     tRNA            complement(759..842)
                     /locus_tag="ACHA6Y_002725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACHA6Y_002726"
     CDS             1370..1870
                     /locus_tag="ACHA6Y_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8988776.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACHA6Y_002727"
     CDS             complement(2060..2404)
                     /locus_tag="ACHA6Y_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM8988777.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT          776 a          476 c          412 g          821 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agatt
//
LOCUS       DAYYHX010000264         2452 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000264 DAYYHX010000000
VERSION     DAYYHX010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.267"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1461)
                     /locus_tag="ACHA6Y_002728"
     CDS             complement(<1..1461)
                     /locus_tag="ACHA6Y_002728"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8988778.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSK"
     gene            1765..2436
                     /locus_tag="ACHA6Y_002729"
     CDS             1765..2436
                     /locus_tag="ACHA6Y_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8988779.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT          821 a          451 c          349 g          831 t
ORIGIN      
        1 ctttgaagga tcttgttcac ctaatttcaa tactgataat ttatcctgca actctaccaa
       61 taacggatca ggacttggca caaaattttc cccttgatca tcgactgcca gtaaataacg
      121 taaccaagca gcaatagcga taggaataaa tgttagattt tctgcatttc cgtctttttc
      181 gacatacttt ttaattgttt caccatagcg aatcgctact ttttgagaag tatctgttgc
      241 aattcgttgt ggtgtatctg gaatataggg attaggcaaa cgtttattaa ttacttcatc
      301 aataaattgt tttggatcaa taatgcctgg attttcaact actggtaaac cttctaaata
      361 acctattcct ttgattaaac caaccaaatc atcatcctgc atctctgctg aaattgattg
      421 atatcctagc agacaacctg ttactgccaa agcagtatga agtggattca agcttgtcgt
      481 aaccttcatg atgtctgctt tatcaaccgt ctctctatct gtcataatca caccagcctt
      541 ttccaattta ggccggccat ttggaaaact atcttcaata actaaataat gaactacctc
      601 agtattagtg aaagcagcaa tattggtatg tttttttgtg tgaatgatat ctaaatcctc
      661 gagccccatc atcaatagat cttcctttac attctctgaa ggatttggcg taattcgatc
      721 aatcatactc caaggaaaag tcactttttc tttatccttt aaataagcaa taaatttttc
      781 agaaacatat ccttcttttt gccaagcttc tgcaatcgta aaaatactat cttgaaaacg
      841 ttgaccattc tgtgagaaat tatcagtaga aaccatcgca atcggcaact ctccagcatt
      901 aaatctgcta tataacaagg atgttacaat actcatcgta tgttgaggat tgctaggtcc
      961 agatttgata tcagctttga ctaccgaaaa gaattgccct ttagaatctt ttaaactgta
     1021 acctttttca gtaatagtaa acgttgtaaa ctgtaattta ggatcttcaa aatatttaac
     1081 cactctttga tagtccgcta tattcttcgg attgcaatat aaactatcag ccgttactgc
     1141 caaaagtctt tttttgagtt caccatctcc actcataaca atttccaaaa tgtcattgtt
     1201 atagtcatga taagcttttt ccactacttc ttcatcaaat gtttcacaca caactactcc
     1261 tgtttctaat tcattttgat ctgctaagca ttgagcaatt tctgcatgaa atcctcgata
     1321 aagatttcct ccaccaaaat gaagccatac tggatgttgc tggccttctt gcttaacttc
     1381 tttgacatca aaacacggca gtagaatctg catttcattg aattcttcga tagaattttg
     1441 ccaattttta atcgaaacca taattaacct cctcaaactg atatgttagt tttgatacat
     1501 tttatatttt aaaacccatt gtaaccgttg tcaatataaa aagggaataa acttgctttt
     1561 ttctaccata tcagaatgat tgtatttttt attcagaaaa ataaaatact ccttcttttt
     1621 ggactgattt tatcgaaact tattttttct ttttttcatg tattctttta tttttataac
     1681 tagtgtatta cttcaaaatt tgatattata tggcatgaac agaattaaaa tatcagttaa
     1741 aataaaagtt aaggagcatt gtgtatgaca attaaaacta gtctccaaaa tcaagcttac
     1801 caatctattc gccgacaaat tatctatgct gacttagaac ctggaaaaaa agtatcagaa
     1861 aaaaaattag aagaaacact ttctattggt cgtacaccaa tacgtgaagc actgattcaa
     1921 ctccgacaac aaggtcttgt tgatacaatt cctcaaagcg gaaattatat ctcattaatt
     1981 gatttagatt cggccaaaaa tgctcgattt gtcagagaac atttggaacg acaaattatg
     2041 ttagagtgtt gtgccaaaat gaataaccaa aaaaaacgga ttttagaaac tataatttca
     2101 gaacaagaaa aagctgttag tcaaaaaaat gctcgtagtt tttttcataa tgataattta
     2161 ttccataaag cttgttttga aattgcagag cgagaagaag tatggcaatg gttagaaaat
     2221 aacaatacac acttagaacg atttcgatgg ctacgagtaa aaacggaagg attaaagtgg
     2281 gaaaaaatta tggaacaaca ttatcaacta tttgaggcat taacaaaaca agatccagaa
     2341 gaagctaact ttctaacatc gttgcatctt catttaatgc ttaatgagca aaatattgtt
     2401 ttagctactc atccaaaata tttcaaaaat atataacttt taactctttt ta
//
LOCUS       DAYYHX010000265         2423 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000265 DAYYHX010000000
VERSION     DAYYHX010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.268"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1368
                     /locus_tag="ACHA6Y_002730"
     CDS             <1..1368
                     /locus_tag="ACHA6Y_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988780.1"
                     /translation="SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIAL
                     TIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSK
                     QIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDS
                     IIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFL
                     FQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIA
                     AKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGE
                     SWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVD
                     VNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINK
                     KNENK"
     gene            1355..>2423
                     /locus_tag="ACHA6Y_002731"
     CDS             1355..>2423
                     /locus_tag="ACHA6Y_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM8988781.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTM"
BASE COUNT          802 a          368 c          417 g          836 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttgct ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttattta gttgtctcta
     1741 tattttctct gcttatcgga cagtagacat ctactatcgt tccgcaattg ccgaagctat
     1801 cgcaataaca cttgttatcc ctgtattatt ttatgcttat caaatcatct ctggaaaaga
     1861 agaaaagtat ccgtctgtaa agctagcatt gagtatgagt ttgctagtgt acagccatgt
     1921 actatcaaca ttaatgagta cagcattaat tatcattttt atttttattc gtttggtttc
     1981 taaaggtttc aagaatgcag attttattgc tatatttaaa aaattattta gtgcggcagg
     2041 aatgacattg gttctgacga gtgctttttg gtatccgatg tttgaacaaa tgctctacca
     2101 aaaaatcaac aaacctagcg tgacaaattt atatgctcat gcatctaatg tgttcgatag
     2161 tctgactgaa gctatgaaca acgacttaac tacttactca atgggactag ttggtctgtt
     2221 atctttatgt attcccctga ttttatttaa aaaattaaca aacatagaaa aaaagattta
     2281 ttatggtacg tgtttgactt ggctagctac aactagtttg gtgccatggt atcttctgca
     2341 aaatacgcca gccaaattgt tacaatttcc ttggcggatc ttaagcttgc aaataatatt
     2401 ctcttctctg atattaacga tga
//
LOCUS       DAYYHX010000266         2408 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000266 DAYYHX010000000
VERSION     DAYYHX010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.269"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            258..857
                     /locus_tag="ACHA6Y_002732"
     CDS             258..857
                     /locus_tag="ACHA6Y_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8988782.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            906..1622
                     /locus_tag="ACHA6Y_002733"
     CDS             906..1622
                     /locus_tag="ACHA6Y_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFM8988783.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            1636..>2408
                     /locus_tag="ACHA6Y_002734"
     CDS             1636..>2408
                     /locus_tag="ACHA6Y_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFM8988784.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGL"
BASE COUNT          865 a          363 c          477 g          703 t
ORIGIN      
        1 gaatagaata acttaaaagt actgacaaat tttttaaagg ggcaatatta atgttctgag
       61 ataatcaaat cttaccgaaa atatcaaaag tccaatgtga cacaattgga ttttgtcaca
      121 ttcgttaaaa tcgctccatc cttagaggct ctaataggaa atcacttttc ttttagcgcc
      181 ttttttctat agcttgttta aatgtgactt attatctgtt ataatcgtat taagtaacat
      241 ttgagaggtg tctaaaaatg aaaagcgtgg aaccgattcg cgacaaaaaa aagatcgatg
      301 ccatgaaggc cattttggct tccggaaaat atgggcaacg aaatttggtg cttttttcga
      361 tcgggatcaa tacagcctat cgaatctccg atttaagaca gttaaaatta agggatgtgc
      421 tagaaatttc ccgtgggcgg gtgatcgtca aagaacgtct ggccatgaag gaacaaaaaa
      481 cggccaaaca caattcggtc ttcatttcga acaaattgcg aaaagtgatt cttgattatg
      541 ttcaatccga atttcctgag cagttgcaag cacaagactt cagcaagtat ttgtttccaa
      601 gtcgaaaagg agcagatacg cctttgaccc gacaaagcct ttggcggatc attcatgaag
      661 caggaactgc agttgggtta aaagaaattg gtccccattc gatgcgtaag acctttggct
      721 attttttata caagcaagga accaaaacag agatcattca gtcgctgcta aaccattctt
      781 cacaacggga gaccttacgc tatattggaa tcacccagga agacaaagat actgcagtaa
      841 agagtttgga tttatgacag aataatgccc tatataatag aaggaaaaat tcaagaggtg
      901 agaaaatgaa cgaagaaaag cagcctaact ttcctgataa atatcactta tctcgcaaag
      961 agtctgtgta tttattgaag aaaaacattg tagaactggt gtataatgcg gggaaatttg
     1021 aagggttaaa tacaacgttg ttacaaacag aagggattat taagtataat cgggccaaca
     1081 acgtggctgt agatgatgtc ctgaccgtcg ttaacttaaa aagaggcttt gaaatgcttt
     1141 taaatgacgt acaggagccc ctgttagaga caagtaagcg gattaatcgg atcgttgcag
     1201 cagaggatgc gctttttccc ggtgaaatac gaaccggtgg ggtagaatta tcgacgattc
     1261 aaggaagata tgtgccacca atgttaactg aagacgaggt aaacaatcaa tatggtgaga
     1321 taatgaatca agagatatca gatacggaaa aagccctgcg actgtttcta ttcatttcga
     1381 aaaatcaaat attctggaat ggtaatgtgg gtacagcctt gttgattgcg aataagatta
     1441 tgtttagcaa cggtttagga ctattatcaa tacccgaaaa tgtatttatg aaatttaacg
     1501 aacttttatc aaagtattgc aacagtaatc tattagtcaa tgagtcaaat attatatcat
     1561 ttttatatga acagtgtatc tttggtatta attatttgaa aataatccaa catacaattt
     1621 aagaggtata agtaaatgat gactatagac gaagcaaaac aattagcaac ttatttttca
     1681 gttggttcag cgggatatat tttagacttt actaatgtaa cgtggaacat atttacaaag
     1741 aaaatcgtag ggattgaaat acaaaatcat tatgggggct caaagggagt atcctttcaa
     1801 aagtttctgc ttgacgatca atttgatgat tcgttaaaga atcggttaat ccaatcttta
     1861 aaaggtatta tggatcgaga taaaaaatat gaatctactg aaatgaatcc catagttcag
     1921 ttgctttttg aggaagtatt taaagaagat acttcaactg cattaagaaa aatgaaattt
     1981 tcaagcgaga ttgatttact ttatattcgg cagctaccca ttaggattca aacagatatt
     2041 aaaaacaagg attttgactc tgttttgaca aaagcaaata ctttaataga tgaagttcta
     2101 aaatttatta ttgaaaatac cgaaggaccg agtgttaata ttaatttatt taagtcaaaa
     2161 gaattaagaa aagaagtatt tggccgcctc aatattaaaa ctgaaaaaga tatggataat
     2221 agaatcaaag cccttgttgg agcactcaat acattatcag ataaaatact cgaaatgaga
     2281 aatagtcaag gagatgctca tgcacatggc tcaaatagaa tagtaattaa ggaagaagaa
     2341 gcaattcttg ttgcaaattc ttctatggtt ttgtgcgagt atgtgtttac taagtataaa
     2401 ggattaaa
//
LOCUS       DAYYHX010000267         2401 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000267 DAYYHX010000000
VERSION     DAYYHX010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.270"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            98..2251
                     /locus_tag="ACHA6Y_002735"
     CDS             98..2251
                     /locus_tag="ACHA6Y_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM8988785.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          819 a          424 c          533 g          625 t
ORIGIN      
        1 taggtaaaga atttcttgct ccgcccattt tttctttttt attaagaagt ttttgggtac
       61 aatgaagcat gaatgtaaat aggcggaagg ataggaaatg atgaaagatt ttatgaaaaa
      121 aatattaaat acttcttggt tagaaaaaat aagaaaaggt gcacaagaac ctaagaacaa
      181 aacgtttcgc cggtcccatg ttccttttcg cttaaatttt ctttttttca ttatatttac
      241 gttatttgtt gcgttgattg ctagattggg ttatttgcag atcgtgaacg gcgaagagat
      301 ggaggcgcga gtgaagtcta cttctacgat tacggtccaa gaaagtactc ctcgcggaat
      361 gatctacgac tccacaggaa aagctttagt tacgaataaa gcgaatcagg ccatcacata
      421 tacgagagga acacaagtta cggctaaaga tttgcttgaa atcgcaacga agctgaactc
      481 gctgatcgat gttccggtag atgaaaatct gacagataga gataaaaaag atttttggct
      541 ggctgatccc aaacatttga aagaagccac aaaaagactt tcggcaaaag aaaagcaatt
      601 agggaacagc gaacaatata gtgcgatggt caataaagtc acagctgatg agattaattt
      661 aaacgaggag caattaaaaa ttgctaccat ctttaaacgg atgaatagtg catatgaatt
      721 aaacactgtc tatatcaaaa actctggtgt tactgatcaa gagttagctg ttgtggcaga
      781 acgcgcttct gaacttccag gagtttcgac tggcacagac tggacacgag aatataatgc
      841 agcaagttca ctgaaaagta ttcttggcag tgtgacgact gaaaagcaag gacttccagc
      901 agatgaagca gagaaatacc tagcaaaagg ttattctcgg aatgaccgtg taggacaaag
      961 ttatttggaa aaacaatatg aagatgtcct gcaaggaacc aaaacccaat atgaagtttc
     1021 ccttgataat gaaggaaatg tttcgaatca aaaagaaatc ttctctgggg aaaaaggttc
     1081 taatttaatg ttatctatga atgcagaatt ccaatcaaaa gtagaagaaa ttttgaaaag
     1141 gaattatcaa acattgatca acaacggcaa agcacagtat tcgcctggtg cttatgcagt
     1201 ggctatgaat cctcagacag gagaagtgct agcaatgact ggtttcagtc atgaacaagg
     1261 ttccaaagaa atcaccgaga atgctttagg cacgattact agcgcctttg atccagggtc
     1321 tgtagtcaag gctggtactt tgacagcagg ctgggcaagt aacgcgatca gcggaaatca
     1381 agttttgatt gatgagccga ttcgtctgca aggtgcttca gaaaaaagct ctgtctttaa
     1441 tcgaagcgga caagttgctc ttgatgcagt caaagcatcg gaattgtctt ctaatacgta
     1501 tatgatcaaa gtggcgttaa agatgttagg actagactat acacctggaa tgggactacc
     1561 tagtttagat gaagaagcta aagcttatca gcaattgaga gactctttca aagagtttgg
     1621 tcttggtaca acgactggga tcgatttgcc gaatgaatct cctgggatct cacgttctgt
     1681 tgattatatg aagaagttca atgcggataa tggcaaagaa tggtatgctc caggtaactt
     1741 taccgatttg gcatttggac agtttgacac atatacacca atccaattag ctcaatatgc
     1801 ttcaacagtc gctaatggcg ggaaacgagt acagccgcac ctagtcaaag cgatctatgg
     1861 caatgacgaa aatggaaact tgggtgaagt gaagaaagaa attggcacaa cagtagaaaa
     1921 tacggtcaat atttctgcag aaaatatggc gatcctccgt gaaggattcc accaagttgt
     1981 ccatggtacc gatccttaca caacagccaa accattagcc agtgcgaaaa tggacctttc
     2041 cgcaaaaaca gggactgcag aaacggttgc ggaaggtcat ccagatattg cgacagtcaa
     2101 tagtaatatc gtggcatacg gaccaacaga caatccggaa attgccatca gtgttgtctt
     2161 gccgaactta ttagacgaac aagaccacat aaacttgacg attgctaaag aaataatgga
     2221 cgcgtattat gatatgttca tggcaaaata aaaaagaggt cgtgaaaaag ccgcttagtt
     2281 tcgagtatga agaaaaattt tgtgaaaata gcttctcaca tttttgctta aatttgactt
     2341 cataccgaga aactggcttt tgaacaccgt ttactcggca aaaaaggttg tgacaaaaat
     2401 t
//
LOCUS       DAYYHX010000268         2375 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000268 DAYYHX010000000
VERSION     DAYYHX010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.271"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(4..249)
                     /locus_tag="ACHA6Y_002736"
     CDS             complement(4..249)
                     /locus_tag="ACHA6Y_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988786.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="ACHA6Y_002737"
     CDS             complement(266..1117)
                     /locus_tag="ACHA6Y_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8988787.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="ACHA6Y_002738"
     CDS             1283..2287
                     /locus_tag="ACHA6Y_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM8988788.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          756 a          402 c          478 g          739 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aacaa
//
LOCUS       DAYYHX010000269         2356 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000269 DAYYHX010000000
VERSION     DAYYHX010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.272"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..1026
                     /locus_tag="ACHA6Y_002739"
                     /pseudo
     CDS             56..1026
                     /locus_tag="ACHA6Y_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          845 a          328 c          470 g          713 t
ORIGIN      
        1 atttgtgcta cactcaaaat agttccgacc aaagaattat aaggagtgag tgcaaataac
       61 ctatacctat cttacaacgg atgaacttgt aataatagag tcttatttga aaattaacca
      121 atctgttgct aaaactgcgc attgcttgaa tcgttcaagt caaacgatcc ataaagtata
      181 tctgttcttc aagcaaagaa aatcagcctt agaatactat caacagtata agaaaaacaa
      241 atcaaactgt ggtagacgtc cgcttgtttt acccgaggaa caatcagaat atattcaaag
      301 aaaggttgtt caaggatgga cacccgatgt gattgttggt cgtgcagcgt ttcctattag
      361 ttgttctgct cgtaccattt atcgtatgtt caaaaagggg ctatttgatt cttctgactt
      421 accgatgaaa ggcaagcgta aaccgaatgg acatcaagaa agacgtggaa aacaaacttt
      481 ccgccgctct attcatgaac gtgaaaagga ttatagccaa ttctcaaatg agtttggtca
      541 ccttgaaggt gacactatcg taggtctgaa acataaaagt gctgtaatta ccttagttga
      601 acgattatca aaagttatca tcacattgaa accgtgtggt agacaagcga ttgatattga
      661 aaaaaaatta aatcaatggt ttgaatctgt accgaaaaac ctattcaaat ccatcacttt
      721 tgattgtgga aaggaatttt caaattggaa acagatcagt aatgtcaatg atattgccat
      781 ttatttcgct gatccaggaa cgccgtctca aagaggccta aacgagaatt ctaacggatt
      841 gttacgtaga gatggtttat tgaaatctat ggatttcaat tcagtagatg aattttttat
      901 tcaatctgtc gcatctaaac gaaataatat tcctagaaaa tcactggatt atcgaacgat
      961 tatcgaacac ctttggaagt atttttgagt tacgtaagta ttgatgatct gtctaattta
     1021 atttgacaat taagaaagta ttaacttgtt ttgtgaaaaa gagaatttga aaaacgattg
     1081 gcgaacaaat gctcggtagg tatggtataa atacagagct aattggtgtg atgaaatcag
     1141 atattcgcgt gagattcagt aaagtaggag ttcgaaaaca gctaggtgac aaacagtatt
     1201 gctttggtac ttcggaatgg tttatcaaat tcttatcaac agttcacttc tctttatgtt
     1261 tgagtgatga ttcgttaggt tattgcttgg aaagaccgga tgagagtaga agagtttaat
     1321 tttataaagt gaaaagaggt aatgtacgat atacgtaggg ctgaaccaat agaggataag
     1381 caaacgatgt tacaggcgca aaattcatag cttactgttt tggagataac agaagaggaa
     1441 ataatgcaga agtaaggcga aaaaaggacg gaagcctaac agcatatgaa ataaagaaaa
     1501 atattataac tactgataaa tcggtattga gcaaagtact aataggagtc aaaaacattc
     1561 ataagctatg agtgtttctt ggctactttt ttatttttat gtatctacaa aagtttccag
     1621 gggcagaacc tgtgattatt aatggaagaa cctcaagtat tgtcttaaat cattgttatg
     1681 aaaatggatt aaaagagttc atcaaggtgt gattaacaag ttacttttca ttacatcgaa
     1741 tttttcagaa gttgcttata ttggtgatga tattaatgat ttatcatgta tgacatctgt
     1801 gaagaagcgg gaggaatagt agaatgtcca aaaaatgcca caagaaatga gattgaatta
     1861 tctgatttta ttgtaactca cagtggtggt gacgaatctg tgatagagtt tattgagtgg
     1921 atattggaat cgatatagtg tagtagtaca gaaaaattat catacaatta atactaggca
     1981 taagaatatt ggcatattct agagttaaaa gtaactattc gaaaaggata aggttagaat
     2041 tttttgtttt gccagtggaa gaatataaaa agggcattgg aaattaatat attagaatgc
     2101 taagcggaga aaaaaatata tgaaaagtac gtccttgtga tgggatggaa aaatacactt
     2161 atttcaaaca acgataatat aattatgtga accgaagtat actcgcttct aattcaatcc
     2221 tcaaatcccg acagtcgcga cttcaaaaaa gcgtatagtt ttaatgtatc cccatatgat
     2281 ccgaaaaatg gtaatttaca attaacagtt tgatgatgtt gaagatgtgg aaatgctgaa
     2341 attaccatga tgagaa
//
LOCUS       DAYYHX010000270         2316 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000270 DAYYHX010000000
VERSION     DAYYHX010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.273"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..542
                     /locus_tag="ACHA6Y_002740"
                     /pseudo
     CDS             288..542
                     /locus_tag="ACHA6Y_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            612..1124
                     /locus_tag="ACHA6Y_002741"
     CDS             612..1124
                     /locus_tag="ACHA6Y_002741"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8988789.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1121..2158
                     /locus_tag="ACHA6Y_002742"
     CDS             1121..2158
                     /locus_tag="ACHA6Y_002742"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8988790.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          842 a          419 c          465 g          590 t
ORIGIN      
        1 ataaattatc taaagagaat taagttttga ttatgtaata agttttggta ctaaaaaaga
       61 cttgatctaa ttagaccaag tcttttgtta gtgttatatt aataacaaaa taaaaataag
      121 aagtcgctca ctccccgacc aaagtttgtg agcaaaataa acagtcgatg tttgtttctt
      181 aattgtatat cttgatatta aacgatttaa gattattctt caagatatat attcgcaaat
      241 gtaattggaa aagcgacttc ttaaacaaaa ttaaggagtc gctttttatg gcaaaaataa
      301 cgaaaaatga gcaagaaaat ttggtggaaa attacacaaa aaaaccggcg tactctaata
      361 gccggttaga cgcacatttc gtgtgcactt ctaatccgaa attgagcttt gacgctatga
      421 cgatagtcgg aaaccttaat aaaaacagcg ctaaaaagct atccgatttt atgagtttag
      481 atccacaaat tagactttgg gatatacttc aaacaaaatt taaagctaaa gcactacaag
      541 accccctatg ttaggctagt tgtcgtatag gataataagt tataataaag actatacgga
      601 ggatgattac catgacaaat aaaaacaatg aacgttactc taccgaactg aaagcgacag
      661 ccatcaagaa aatgatgcca cccgaaaata gggggattcg ccaattaagt caaactttgg
      721 gcattccaga agcgactctt tattcttggc gtaaaaaagc tagaattcaa gggaatacta
      781 cacccggaac gaaaaaaaat gtcgaaaaat ggtcaagtga agataaattt ttaattgtga
      841 tggaatcatt ctcaatgaac caagtagaac ttgcggaata ttgccgaaag aagggcttat
      901 ttaaagaaca aattgaggct tggaagaaaa cgtgcttatc tgcgaatgaa caggaagaaa
      961 atcgaacgag agaacttgca actgaactaa aagaagagaa aaaacaagcg cgtcaattag
     1021 agaaagaatt acgtcataag gaaaaagcat tagcggaagc cgctgctctc ttattactaa
     1081 gaaaaaaagc acacgcgatt tggggggacc aagaggaaga atgatttcat cttcagatcg
     1141 caaacaagct gttgaactta ttcaagaagc caatcgcaat ggggcccgtc tcacctatgc
     1201 ctgtaatgag ctgaatatta gcgttcgaac ctacgaacga tggacgcgtg aaggtacgat
     1261 agcgcatgat caacgccctt tggcgaaacg tcctgtaccc aaaaataaac tgacggatca
     1321 agaacgcgaa aaaataatag aaaccgtttc aaaaaaagaa tttatgaatt tgccacctag
     1381 ccaaattgta ccaaaacttg cggattgctc gatttatatt gcatcagagt ctagcttcta
     1441 tcgtgtattg cgagaaaaaa atatgcaaca tcaccgtggc cgaagtcaag tttcacaaaa
     1501 acgcataccg ccaagtcacc ttgcgacgaa gccaaatgaa gtttggactt gggatattac
     1561 ttggctaaaa ggtccaataa aaggactctt ttaccggtta tatctcatca ttgatttatt
     1621 tagccgtaaa atggtaggct gggagatttg ggaatcagaa gaagctcgtc atgccgaaaa
     1681 acttattaaa cagacggtgc ttaaggaacg tatccaagga cagccgttgg ttttacactc
     1741 cgataatggc agccctatga aagccgcgac ttttcaagtc ttactcgaaa aattaggaat
     1801 tcaaagttct tattcaagac cacgagtgag taatgacaat ccttattcgg aagcggcttt
     1861 ccgaacgtta aaatatcgac cagaatatcc caataacggc tttaaaacaa tagatgatgc
     1921 tcgaaaatgg acgaaagagt ttgtcgattg gtataacgat tctcatttgc atagtgggat
     1981 tcaatttgtg tctcctgcag cgtgtcatga aggacgacat ctcgctattt tagagaaacg
     2041 aaagcaggtt tacgaggacg ctaaaaagcg ccatcctgaa cgctggtcaa aaggtgttcg
     2101 aaattggact ccacatcaat cggtggcgtt gaatccttta aaagaaacag aaagttaaca
     2161 ggagcataat ctttcaagat tatcaaataa ccctcgtcac ccaatagggg aaaaagttat
     2221 tcttccccta ttggataacg aggccaaccg accataggga ggttctgctt tttgataggt
     2281 ggaaaataaa aatacgacaa ctatattgac aaacac
//
LOCUS       DAYYHX010000271         2283 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000271 DAYYHX010000000
VERSION     DAYYHX010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.274"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..1954
                     /locus_tag="ACHA6Y_002743"
     CDS             92..1954
                     /locus_tag="ACHA6Y_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8988791.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1954..>2283
                     /locus_tag="ACHA6Y_002744"
     CDS             1954..>2283
                     /locus_tag="ACHA6Y_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8988792.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          819 a          341 c          451 g          672 t
ORIGIN      
        1 aagctataag ttgcacttag ttaagtgcaa acatttcata ttgtcgttag tttagaaaac
       61 gcttatatta gcaatgtaag gagagatgat aatgcagcag ctaacaaaaa gagaaaggca
      121 gcttttgatt tacttggtgg aaatgtctga accggtaaca atcaaaaagt tggctgaaaa
      181 agaaaatgtt agtgttcgga cgataaaata cgatctagat gatataagag agtggctgaa
      241 tgagcggaag caagtcctat atagcaaacg tagtcaggga atttggatga atattagtag
      301 ctctgatcga atcaaattaa aaagcgaatt gatggatgtt aatcgttttg aaatatttgc
      361 agatcaaagt ctccgaatca aacgtttact aattatgctt ttagtaacaa aagaagctat
      421 tactggaatg aaaatggcca acgcattaga tgtcagcaaa gatacgatca tgaatgattt
      481 agatgtgctg gaaaataatt ttcttaagga aggattgacc ttgaatcgtc aggcgcgaaa
      541 gggtttttgg atcataggag aagaacgtct agttcgatta acgattgaag agattcttca
      601 aaaagagttc acggattatg acatttataa attaatgtca ttgttattga atggcggtga
      661 gactgaatac tttgaaatgt attctgctac tgctacacct atccaagagg tatttaatca
      721 agtaatcatt cgaatgcgac acttactgga atttgaaaat ctagagaaac tgaattacgc
      781 ggaattacta aatattctga ttcgagtgac aattgctacc gttcgcttac gcaaagaggc
      841 tacgattggt cgttatcaat tggttgctga acaagaatta gacaaaaacg atctttctta
      901 tcgcgtcatg cagcaggtct ttgattatta tcagttgcca ctgttcgagg atgaatatca
      961 ttatatctat agtgatacat tcgacacgat tcaccagcaa gacgtcatga aactagttga
     1021 gaatgtgatt tataaagtaa gtaaagcaat caaccattct ttttatcaag atccacaatt
     1081 gttgacgaat ctgtatgctc atttatccat gcgattatcg agaaagaaaa aatttgtcaa
     1141 tgaatataat ccttttaaag aagatatcaa gcagaaatac ccggaattat ttgatgcgat
     1201 caagcaagca gttcgacaag aaataagtga acaaaatatt ctgatcaatg attctttcgt
     1261 ggcatatatt gccttacact ttttggtttc ctacgaaaga gaagaagatc tacgaagcgt
     1321 tagagccgtc tacatttgtt caacagggtt gggtgtgact agtctgatca agcaaaagat
     1381 caatgaagaa ttaaataata tcactatcgt ggcatttgct tcagtattga atgcgcaaga
     1441 aatgattgct accaaagatc ctgatttggt gattagtatt tttccaatta atggcatcga
     1501 tcgtcctttc atcaaagtcc atccattacc cactgaacag gacttgaaaa aaattcaaca
     1561 aataataaaa aaaatacttg cttcttccaa tcagcaggaa aggcataact tgaatcttag
     1621 agaaacgtcc atcaataaag atactttaga agactttagc aaggaattga ttttgaaagc
     1681 ttatacgatt tatgaacgtt tacttgtcat tttggaagca gaattgatcc aagaatatcg
     1741 agaagctttt ttgcttcatg tattcttaat ggttcatcgt atcacatttg atcaacaata
     1801 tatggcagaa ggtcgtcaag tagaccagat tattttagac aaaggaacac tggtaggaaa
     1861 aatcgaagca gtttttgcag aaaataattt aggaatcaat cagtcagaaa tcagtgcatt
     1921 attctcatat ataaaagaag gagcgtcaag ttagtgaaaa tgtcagacga ggctttaaaa
     1981 attatcgagg aaagtcaaaa tcgggaaaag ttaaaacaag tgattgatct aattgaagag
     2041 cgattagtag aacatgctat tgtaccaacc gagttgcagt ggaccattct aattaatcac
     2101 ttgaatgaga tgctgaaacg ttctcaaaga aaggaaacga ttcccgtagt cgatcgggat
     2161 ttgttcaaag aagtatccaa agaagccata actatttctg atgaaatagt tagaagtatc
     2221 ggtaatttaa ccgatgatga aatatatgtt ttgtcgattc attttgaaac agcaaaaaat
     2281 aat
//
LOCUS       DAYYHX010000272         2266 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000272 DAYYHX010000000
VERSION     DAYYHX010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.275"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(30..101)
                     /locus_tag="ACHA6Y_002745"
     tRNA            complement(30..101)
                     /locus_tag="ACHA6Y_002745"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66..68),aa:Glu,seq:ttc)
     gene            complement(113..186)
                     /locus_tag="ACHA6Y_002746"
     tRNA            complement(113..186)
                     /locus_tag="ACHA6Y_002746"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(150..152),aa:Ile,seq:gat)
     gene            complement(199..269)
                     /locus_tag="ACHA6Y_002747"
     tRNA            complement(199..269)
                     /locus_tag="ACHA6Y_002747"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(235..237),aa:Gly,seq:tcc)
     gene            complement(274..346)
                     /locus_tag="ACHA6Y_002748"
     tRNA            complement(274..346)
                     /locus_tag="ACHA6Y_002748"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(311..313),aa:Phe,seq:gaa)
     gene            complement(350..422)
                     /locus_tag="ACHA6Y_002749"
     tRNA            complement(350..422)
                     /locus_tag="ACHA6Y_002749"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(387..389),aa:Asp,seq:gtc)
     gene            complement(441..514)
                     /locus_tag="ACHA6Y_002750"
     tRNA            complement(441..514)
                     /locus_tag="ACHA6Y_002750"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(478..480),aa:Met,seq:cat)
     gene            complement(527..616)
                     /locus_tag="ACHA6Y_002751"
     tRNA            complement(527..616)
                     /locus_tag="ACHA6Y_002751"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(578..580),aa:Ser,seq:tga)
     gene            complement(639..710)
                     /locus_tag="ACHA6Y_002752"
     tRNA            complement(639..710)
                     /locus_tag="ACHA6Y_002752"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(675..677),aa:Glu,seq:ttc)
     gene            complement(732..819)
                     /locus_tag="ACHA6Y_002753"
     tRNA            complement(732..819)
                     /locus_tag="ACHA6Y_002753"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(783..785),aa:Ser,seq:gct)
     gene            complement(844..917)
                     /locus_tag="ACHA6Y_002754"
     tRNA            complement(844..917)
                     /locus_tag="ACHA6Y_002754"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(881..883),aa:Ile,seq:gat)
     gene            complement(931..1001)
                     /locus_tag="ACHA6Y_002755"
     tRNA            complement(931..1001)
                     /locus_tag="ACHA6Y_002755"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(967..969),aa:Gly,seq:tcc)
     gene            complement(1003..1078)
                     /locus_tag="ACHA6Y_002756"
     tRNA            complement(1003..1078)
                     /locus_tag="ACHA6Y_002756"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1043..1045),aa:Asp,seq:gtc)
     gene            complement(1097..1170)
                     /locus_tag="ACHA6Y_002757"
     tRNA            complement(1097..1170)
                     /locus_tag="ACHA6Y_002757"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1134..1136),aa:Met,seq:cat)
     gene            complement(1183..1272)
                     /locus_tag="ACHA6Y_002758"
     tRNA            complement(1183..1272)
                     /locus_tag="ACHA6Y_002758"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1234..1236),aa:Ser,seq:tga)
     gene            complement(1296..1369)
                     /locus_tag="ACHA6Y_002759"
     tRNA            complement(1296..1369)
                     /locus_tag="ACHA6Y_002759"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1333..1335),aa:Ile,seq:cat)
     gene            complement(1383..1456)
                     /locus_tag="ACHA6Y_002760"
     tRNA            complement(1383..1456)
                     /locus_tag="ACHA6Y_002760"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Met,seq:cat)
     gene            complement(1484..1556)
                     /locus_tag="ACHA6Y_002761"
     tRNA            complement(1484..1556)
                     /locus_tag="ACHA6Y_002761"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1521..1523),aa:Ala,seq:tgc)
     gene            complement(1576..1649)
                     /locus_tag="ACHA6Y_002762"
     tRNA            complement(1576..1649)
                     /locus_tag="ACHA6Y_002762"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1613..1615),aa:Pro,seq:tgg)
     gene            complement(1659..1732)
                     /locus_tag="ACHA6Y_002763"
     tRNA            complement(1659..1732)
                     /locus_tag="ACHA6Y_002763"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1696..1698),aa:Arg,seq:acg)
     gene            complement(1743..1828)
                     /locus_tag="ACHA6Y_002764"
     tRNA            complement(1743..1828)
                     /locus_tag="ACHA6Y_002764"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1792..1794),aa:Leu,seq:taa)
     gene            complement(1845..1916)
                     /locus_tag="ACHA6Y_002765"
     tRNA            complement(1845..1916)
                     /locus_tag="ACHA6Y_002765"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1882..1884),aa:Gly,seq:gcc)
     gene            complement(1928..2000)
                     /locus_tag="ACHA6Y_002766"
     tRNA            complement(1928..2000)
                     /locus_tag="ACHA6Y_002766"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1965..1967),aa:Thr,seq:tgt)
     gene            complement(2018..2099)
                     /locus_tag="ACHA6Y_002767"
     tRNA            complement(2018..2099)
                     /locus_tag="ACHA6Y_002767"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2063..2065),aa:Leu,seq:tag)
     gene            complement(2107..2179)
                     /locus_tag="ACHA6Y_002768"
     tRNA            complement(2107..2179)
                     /locus_tag="ACHA6Y_002768"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2144..2146),aa:Lys,seq:ttt)
     gene            complement(2183..2255)
                     /locus_tag="ACHA6Y_002769"
     tRNA            complement(2183..2255)
                     /locus_tag="ACHA6Y_002769"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2220..2222),aa:Val,seq:tac)
BASE COUNT          611 a          629 c          538 g          488 t
ORIGIN      
        1 agaaaaagat actcaaaaag tatcttccat gacccgtacg ggattcgaac ccgtgttacc
       61 gccgtgaaag ggcggtgtct taaccgcttg accaacgggc caataaaata aatgggccta
      121 aatggactcg aaccatcgac ctcacgctta tcaggcgtgc gctctaacca gctgagctat
      181 aggcccataa aaactaaaag cgggtgacga gaatcgaact cgcgacaaca gcttggaagg
      241 ctgtggtttt accactaaac tacacccgca taatggcgcg ggacagaatc gaactgccga
      301 cacatggagc ttcaatccat tgctctacca actgagctac cgagccaaac ggttccgacg
      361 ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta caccacggaa
      421 ccaatgtttt tagtttttaa ttgcgggagc aggatttgaa cctacgacct tcgggttatg
      481 agcccgacga gctaccagac tgctccatcc cgcgataata tgaaaaagga ggataaggga
      541 ttcgaaccct tgcacggttt tacccgcctg acggttttca agaccgttcc cttcagccgg
      601 acttgggtaa tcctccatgt aaaactatat taaattaatg acccgtacgg gattcgaacc
      661 cgtgttaccg ccgtgaaagg gcggtgtctt aaccgcttga ccaacgggcc aggtaaaaca
      721 aaatattgaa acggagaagg agggatttga accctcgcgc cggtttcccg acctacaccc
      781 ttagcagggg cgcctcttca gccacttgag tacttcccca aaaccaataa gattttgtac
      841 taatgggcct aaatggactc gaaccatcga cctcacgctt atcaggcgtg cgctctaacc
      901 agctgagcta taggcccata aaaaactaaa agcgggtgac gagaatcgaa ctcgcgacaa
      961 cagcttggaa ggctgtggtt ttaccactaa actacacccg catggcggtt ccgacgggaa
     1021 tcgaacccgc gatctcctgc gtgacaggca ggcatgttaa cccctacacc acggaaccag
     1081 tgtttttagt ttttaattgc gggagcagga tttgaaccta cgaccttcgg gttatgagcc
     1141 cgacgagcta ccagactgct ccatcccgcg ataatatgaa aaaggaggat aagggattcg
     1201 aacccttgca cggttttacc cgcctgacgg ttttcaagac cgttcccttc agccggactt
     1261 gggtaatcct ccatatgaaa cttacaacgc cacaatggac cttgtaggac tcgaacctac
     1321 gaccggacgg ttatgagccg tctgctctaa ccaactgagc taaaggtcca ggctcttaaa
     1381 aatcgcggcg gaggggatcg aacccccgac ctcccgggta tgaaccggac gctctagcca
     1441 gctgagctac accgcgaaaa agatattcat tatttaaatg aaatggagcc tagcgggatc
     1501 gaaccgctga cctcctgcgt gcaaagcagg cgctctccca gctgagctaa ggccccgtgt
     1561 ttaaatttat aattatcggg aagacaggat tcgaacctgc gaccccttgg tcccaaacca
     1621 agtgctctac caagctgagc tacttcccgt ttaattaacg cgcccaagag gagtcgaacc
     1681 cctaaccttt tgatccgtag tcaaacactc tatccagttg agctatgggc gcatatttga
     1741 aatgccgagg accggaatcg aaccggtacg gtgatcactc accgcaggat tttaagtcct
     1801 gtgcgtctgc cagttccgcc accccggcgc gattgctttg gcaaagcgga aaacggggtt
     1861 cgaacccgcg acccccacct tggcaaggtg gtgctctacc actgagctat ttccgcgtgt
     1921 ttaaagatgc cggctaaagg acttgaaccc tcgaccctct gattacaaat cagatgctct
     1981 accaactgag ctaagccggc atgtaaaact taaattatgc gggtgaaggg acttgaaccc
     2041 ccacgccgta aggcgctaga tcctaaatct agtgcgtctg ccaattccgc cacacccgca
     2101 aaaatatgag ccgtacaggg ctcgaacctg tgaccctctg attaaaagtc agatgctcta
     2161 ccaactgagc taacggctca tatggaggtt aacgggatcg aaccgctgac cctctgcttg
     2221 taaggcagat gctctcccag ctgagctaaa cctccatgga taactt
//
LOCUS       DAYYHX010000273         2262 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000273 DAYYHX010000000
VERSION     DAYYHX010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.276"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..>2262
                     /gene="acm"
                     /locus_tag="ACHA6Y_002770"
     CDS             157..>2262
                     /gene="acm"
                     /locus_tag="ACHA6Y_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM8988793.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVII"
BASE COUNT          887 a          346 c          441 g          588 t
ORIGIN      
        1 ttttttggca tatttatttt ttataaatat ttgtaaaata actatcaatt ttttagttaa
       61 aagattggaa atgagtgggg gaggaattga agaaaggatt tttgagagat gatatagtag
      121 gttttatttt tagttaaaat aggggagata gaaaaaatga aaaagtgtag aaatattatt
      181 ttatcaatgt tatttattat tactaatata acatcactga ttcctgttca tgtctatgcg
      241 gatgcaggca gagatatcag cagtaatgtc acttcgttga ccgtggaccc aacgaatatt
      301 acagatggag gaaacataaa agtaaaattc tcgtttgacg aaaaaaaaca aaatatccaa
      361 ccaggtgatt atctttggat caattggcca agtgaaggaa atattcgcgg tgaaggattc
      421 caaaaagaaa tcccattaat gattgaaaat aaaaatgttg gaactttaac ggtcagaaaa
      481 gattctgcgc aggttgtgtt taatgaaaat attaaaaacc tagactcagt agaaggttgg
      541 gggcaatttg agatacaggc cagaaacgta accgataccg gtgaagaaaa tacgggacca
      601 ttcaccgtga cgagcggtga taaaacagct actgtaaatg tgacaaagcc agcttctggt
      661 tcttcttcta gcgtttttta ttataaaaca ggagacatgc tacctgaaga tactaagcac
      721 attcgatggt ttttgaatat taacaataac ggcacttatg ttgaacaacc agttaaaatc
      781 agtgatgaaa tccaaagtgg acaaagatta gatccaagta cgtttgaaat taatcaaata
      841 cacttaggtg aacagaaggt ttatagagga gaagagggaa tccaacaatt tttacaagat
      901 ttccccagcg ctacatttaa tttttcagta actgataatt acattgaaat aactatacca
      961 aaaaactttg tcaatcttag gaaaataatg gtgagttata agacgatcat agaaaaccca
     1021 gaacaaataa attttgagaa tcattcggaa gcttggttta aagaatttaa taaacctgct
     1081 gttgatggag aaagtttcaa tcatacagtt aaaaatattt cagcttctgg tggagtcaac
     1141 ggtacagtta gaggcgaatt gaagattttc aaatatatca atgacacaga gattggtatc
     1201 ccaaatgtga ctttcgaatt aagacgagca gatgaacagc ctattcaagg acagtctagt
     1261 atcctgttga cttcgaatga acagggagaa gctagtatca aaggcctgca agtaggagac
     1321 tatgttgtaa aagaaaaaga agcacccaat tggattgatt ttgatccact aagttcaaat
     1381 gaactcaaat tctccataaa cgaaaatgat acagagggag tcagtttacc tatttataac
     1441 aagaaaaagg ttacgaacat cacagcaact aaaatttgga acggtggaac cactccaaga
     1501 ccgagtattt attttaaatt attcagagca agtacaaaca attgggaacc agttcctgat
     1561 gcagaaacaa aacgattaga caatggaata acttcagtga cttgggaaga tatccaacag
     1621 tatgatgata gtggaaacga atatacgttt aaagtgcaag aagtagatca aaacggaaat
     1681 gattatgtcc cttcaggtta tcaaaaaatt gaaaacgggc tagtcgttac aaatgagaat
     1741 aaagaagtca tttcagtctc aggacaaaaa acatgggaag acaatgagaa ccaagacggc
     1801 aaacgaccga cgacgatcac agtaaatcta ttagccgacg gtcaaccgat ccaacacaaa
     1861 gaagtaagtg aaaaagatga ttggcgctat cgtttcacaa atctgccaaa atacagagac
     1921 gggcaagaaa tcatctatac tgtaacggaa gacaacgtgc ccgaatacag tacgacgatt
     1981 gacgggtata acatcaaaaa cagttataca ccggaaaaaa caaatatatc tataccaggt
     2041 agccatataa caccgtggaa aaattttcca gagaagacag agagagagaa aattagaaat
     2101 ctattctctc aaaaattgca aatatttgct catacaggag aatccagcga acaaagaagc
     2161 aattatatta gtaagagagc tgtacctaaa cagataacaa ataaatcaaa aagcattctt
     2221 cctaaaacaa gtagtaaaaa atctagtttt gctgtaatca tt
//
LOCUS       DAYYHX010000274         2225 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000274 DAYYHX010000000
VERSION     DAYYHX010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.277"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(113..637)
                     /locus_tag="ACHA6Y_002771"
     CDS             complement(113..637)
                     /locus_tag="ACHA6Y_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM8988794.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(630..1103)
                     /gene="tsaE"
                     /locus_tag="ACHA6Y_002772"
     CDS             complement(630..1103)
                     /gene="tsaE"
                     /locus_tag="ACHA6Y_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM8988795.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1226..2206)
                     /gene="pta"
                     /locus_tag="ACHA6Y_002773"
     CDS             complement(1226..2206)
                     /gene="pta"
                     /locus_tag="ACHA6Y_002773"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM8988796.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
BASE COUNT          569 a          540 c          366 g          750 t
ORIGIN      
        1 acaccgttta ttcaaaaaaa atggactgcg acacgacttt tgtcacagtc ccttcgtgtg
       61 tttttctgcc ttctcaaaag gatgacagtt tctcactctt ttcctatttc ttctattcaa
      121 tcaaataact cattttatat acatctaaaa attcacctag atctgttctt gcccctctag
      181 gcatgaccgc ttcgatttgg aagcccattt ttcggtaaag gtgtacggct ctttcattac
      241 gaacttgaac gtcaagctcc aatcgaaaaa gtacacccat ttccttcgcc caactgatga
      301 tttcttctag cataagagtg cctagtccca ttccccaata ttcctgcaga atactgatac
      361 cgacttctcc cacgtgagac aaacgaaatt gactgtctgc cttgacagat gctgttccaa
      421 taatcgtccc ttcatagatt gcaagcagca ataaattatt attgctttcg tatatataat
      481 ccaactcttc ttctaaggtt tctggtggta acaggagttc tgcttcatcc aaaactaaaa
      541 attctgtctc cttattaact tgtcccatca tcaaaagaat gtcttttgca tctcctggca
      601 ctgcttcccg caagatgatc ccaacttctt cactcatgat tttcagccca tttttctaga
      661 attcgctgtt tgaaggtttc actttgctcc ccgtatgaga ttagtttcac ataccgcttt
      721 tctaaatctg tatcactttt ttctaaaatc agttccaaat aatcctccgg cagtgcttct
      781 tctaatagat ttccccattc aacaacggaa agcccatcac cttcaaaata ttcatctaac
      841 cctaaatctg ctccgctagc tgctacccga taaatatcca tatgatacaa cggaatccga
      901 ccttgactat attctctaat aatcgtgtat gtcggacttt tgatcatttg cgttatccct
      961 aatccttggg caattccttt cgtcaacgtt gttttacccg cacctagatc accagtcaat
     1021 atcagattgt cacctggcat tgcggaatga ccaattgcct ctgcgaattg ttctgtcatt
     1081 gttaatcctg atagttcgat catttttcat cttctcccgc tcttctttct ttttgccagt
     1141 atatcgcgta atgaagtttt tttaaagaaa aaatgaacta gtcaagaaca gcagctgctg
     1201 tttttggcta gttcgcgtgt atttgttaat caatcaatgt ttgtgctgct gtgataatcg
     1261 ataatttata tacatcttct tcatttgctc cacgagacaa atcagaaacc ggtttgttca
     1321 agccttgtag aattggtccg attgcttcaa agttaccgaa acgctgagca attttatagc
     1381 cgatatttcc tgactgcaat tctggaaata caaagactgt tgcttgacca gctacttctg
     1441 aatctggcgc tttcagttgt gcaactgaag gaacataaga agcatcaaat tgcaattcgc
     1501 cgtcgatcat gtattgtggc gctagttttt tagcgatttt agttgcttca actactttgt
     1561 ctacttctgg cgatttcgct gagccttttg tcgagaagct catcattgct acttttggtt
     1621 cgatatcgaa caattctgct gtttttgcag aatctacggc gatttcagcc aattcttgcg
     1681 cagttggatt cacgttgatc gcacaatccg aaaagacata tttttcttga tctcttccac
     1741 gtaccatgat catcgctccg cttgtccgac tgatgccagg ctttgttttg atgatctgaa
     1801 gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc agagaccatt ccgtctgcaa
     1861 tccccatata cgtaagcatc gtaccaaagt aattcacatc ttttaaaatc ttttctgctt
     1921 gttctttgga tgcctttcca tttctacgtt caacaaaagt atccaccatt tcatcccact
     1981 tatcgtaatt atcagggctg atgatcgtaa agctagatgt tttgattcct ctggcatgcg
     2041 ctgcatttct tatttcttct tcgttcccta ctaaaatggg ttccacaagt tcttctgctt
     2101 tcaaccgggc agctgcgcct aaaatacgag catcagtagc ttccgggaaa acgattttga
     2161 tcccacggcg aacaatttta aatcttaaac tatcaaacaa ttccacaaga tgacctccag
     2221 acgtt
//
LOCUS       DAYYHX010000275         2209 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000275 DAYYHX010000000
VERSION     DAYYHX010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.278"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..465
                     /locus_tag="ACHA6Y_002774"
     CDS             <1..465
                     /locus_tag="ACHA6Y_002774"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8988797.1"
                     /translation="LILILLVVLVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTN
                     EILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPI
                     FQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            593..1276
                     /locus_tag="ACHA6Y_002775"
     CDS             593..1276
                     /locus_tag="ACHA6Y_002775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM8988798.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1585..1658
                     /locus_tag="ACHA6Y_002776"
     tRNA            1585..1658
                     /locus_tag="ACHA6Y_002776"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1619..1621,aa:Arg,seq:tct)
     gene            1817..2176
                     /locus_tag="ACHA6Y_002777"
     CDS             1817..2176
                     /locus_tag="ACHA6Y_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM8988799.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          735 a          345 c          450 g          679 t
ORIGIN      
        1 ttaattctta ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac tccttatatt
       61 attgaggaca aagaaatgag tccatattat gcacaaggaa atctgatata tgtacgaccg
      121 aaaaatacaa atgagatttt agtcggagat attattacat attatgaaaa ttcaggaaaa
      181 aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt ttatacaaaa
      241 gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat tatcggtagt
      301 cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt tgcatggatt
      361 caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg gaataccttt
      421 gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg ttaaaaacat
      481 aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa acactcatgc
      541 agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta gtatgcgcaa
      601 tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc gtacccagca
      661 aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag cagaagtacc
      721 agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc ttcagcctga
      781 acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg ccacgacaat
      841 cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg agaaagctaa
      901 aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag gtcaagcagc
      961 agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata gtgcaaaatc
     1021 aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg gcgttttgat
     1081 ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga ttccccgtgg
     1141 caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg atcatcccga
     1201 tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa aggaatcaga
     1261 aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg aatcgagggc
     1321 tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg atacaggaaa
     1381 tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt gataacctta
     1441 gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg gccaaaataa
     1501 ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta attatctata
     1561 attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc atccgccttc
     1621 taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt tagaaactga
     1681 ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca aaggcaaata
     1741 aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa aactcatcag
     1801 aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag ttgcaattga
     1861 atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga caagtagggt
     1921 ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt ttaagaacca
     1981 aggagtgtac aatggactat taggagtggg actactttat ggagcttatc tttcttccgc
     2041 atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat tatatggcag
     2101 tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt tggcattgct
     2161 ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaa
//
LOCUS       DAYYHX010000276         2190 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000276 DAYYHX010000000
VERSION     DAYYHX010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.279"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(158..388)
                     /locus_tag="ACHA6Y_002778"
     CDS             complement(158..388)
                     /locus_tag="ACHA6Y_002778"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM8988800.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(482..820)
                     /locus_tag="ACHA6Y_002779"
     CDS             complement(482..820)
                     /locus_tag="ACHA6Y_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988801.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(1748..1864)
                     /locus_tag="ACHA6Y_002780"
     CDS             complement(1748..1864)
                     /locus_tag="ACHA6Y_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8988802.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT          725 a          385 c          298 g          782 t
ORIGIN      
        1 atatagaagt gtgtagctac ttactaaata tacaaaagtt aaaataaaat actctggata
       61 ctctttatac tgcattaaag ttagtaatat taacagcaaa gatatcgaaa aaacaataac
      121 ataatatgtg tcaaaatttt tcataataag aactccctta ctgttgcata ctaacataat
      181 ccaaaaactc ttctacttca gctaaagaca ttccactttc tttagtccaa aaaacagttt
      241 tttctatcga tccttctctg taaactgtaa atgtcggagt cgagttaatt gagtagcgac
      301 tcttgaattc tttccactca ttttcatctt ctctaacttt tgctacattc acgtaataaa
      361 tattttcttt tcccttttct accagcatat cgaatagctt cctgcttttg atgatcgatt
      421 tcttgtgcta catctagtaa attttcatca atttctatat cataaagtat ttcactagtg
      481 tctacatcca caatatatac tttttatcag tagcaatttt tgagtaatcc gttaaaatga
      541 tctcagtctc gtcactttca tcagtataaa tatacgttaa caatgaactc accttttcaa
      601 tcggtgttag ctctaagcct actgtatttt ttactattga gtcattattt accaccataa
      661 cttcgtactg tatataccat cctatttggt ttccatcaat atcagtaatt ggctcactag
      721 ctaatggagt ctccggaaca atattttcct gatatttctt aaactcttct gtagcacgaa
      781 aaaggttatc atgttcctca cttgaaactg gttgagtcat agaaaatgtt acttggctaa
      841 acagagggaa aataaacact acaatcaaca aagtataaaa aaaagccctt ctttttcatt
      901 ttttatcttc caatcacaaa ttgaaaaact tattcctgca actttgagtc tagtatcaca
      961 atcgactgac tacaactatt catttacatt ctactttaag ttcctcctta accattaatt
     1021 ttattaaaga tacttgtaat agtgattctc acaaaatttc atgaaaaata ctttattact
     1081 gtatctaaaa ttccaataca caacttatcg actattatct gaaaaccaaa acttattatt
     1141 ccttgatgtt attctagcaa tagtaactgc ttttttattt ttttgcatat tttttcggcg
     1201 ttacttttta aagttaactt tttcgacaaa tagaaagaga tacgaatcac tgattttttt
     1261 gaagaaaaga aatggagtga gctgaacgtt agagatattg ggagctttcg taactgctta
     1321 ttaaacgata atggttattt gcaaccgtat tgctcgcttc tacatgaaat atttcatgaa
     1381 atatttcgca aaacctttga ttatgtagtt acgtttccat tcttgactaa aaatgtttca
     1441 aaaacgaaca aaggtgttaa aaggtaaagt gatcaatact actggtcaaa ataagaggtt
     1501 gaaagggaat tgaaaaataa ctacagataa tacttttata aacgtacgtg ctttgtggtt
     1561 ctgtagttaa ctatataact ggaattaggg aatggtatca atttgaaaag aaagattcta
     1621 ccaatttatc atatgttaaa aatggaaaat caacagcagt tctttcggaa accttacaca
     1681 aaaaccgatt attgaaaata gccgaaaaac gtttttcggc tattttttta attaagtttc
     1741 cttcttctta ccttttccca ctattccgtt tcacatcttt gatgatcttc agcgcacgtt
     1801 ttcgtgtttt gatatttggg cttttcaagc ggcggtatgc acttgagata tctgtttttt
     1861 ccatatggac gacccacttt cttctttgat tgtagtcatg ctaaaaaaca caaatcgaaa
     1921 caattacgat ttgtgtttta aatggtatca aaagttaaaa tgaaagtcaa cttgttaaaa
     1981 cagtcgatca gactagctga ttgactccta tctctggaaa taaattttca ttattacgtt
     2041 ttagctacct aaaaaaggcc ctcttgtccg ttaactttaa tatctattgt tatagctagt
     2101 ttacctattg aacgtactgt gtgttcgata gaacactaaa aaagaggctg ggacagaagc
     2161 gtttaactcc aagaaataag aagaaattca
//
LOCUS       DAYYHX010000277         2133 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000277 DAYYHX010000000
VERSION     DAYYHX010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.280"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(96..1283)
                     /locus_tag="ACHA6Y_002781"
     CDS             complement(96..1283)
                     /locus_tag="ACHA6Y_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HFM8988803.1"
                     /translation="MQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNTLYLDK
                     SLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAKGLFVE
                     KLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYESAMIQ
                     GFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDYQLPEG
                     SRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEMILNDV
                     NNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTEVNINE
                     YSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVTEYIKN
                     YFN"
     gene            1319..2092
                     /locus_tag="ACHA6Y_002782"
                     /pseudo
     CDS             1319..2092
                     /locus_tag="ACHA6Y_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          651 a          418 c          343 g          721 t
ORIGIN      
        1 gctcaatttt caaaaatggc attactcacc tttttggtga attattaaac taaaaaagat
       61 gaattttttt atgtactata agatgtaggt gtactctagt taaagtaatt ctttatgtac
      121 tctgtaacat cgaatacatc ttctaataaa aatgcacgca ataatctgtc aattagcata
      181 tttaccttga ttggtattac cattttacct aaatttttct cgatatatcc actgaaatga
      241 cctacgttaa cacttgattt taatttatca aatcgactat attcattaat gtttacctcg
      301 gttaagcgta aatccaacat ctcacgtaat aatgcctcat taatattggt tgaatccact
      361 aaatcttgga taaaatcgtc ctcttcacta agctgatatt cattgaggta atcagtaaat
      421 tcccatcctt ctttgataac taattcccca ttatttacat catttagaat catctcagca
      481 tatggctgta actccactgg taattgcgca aatgtgcgat gtaagtcgtt taacaatgat
      541 tgaattaaat tcgaatcacg ttcttctttt atattcatgt attttttgaa gtttgtgttt
      601 aaatactcat aattaatacg actacgacta ccttcaggta attggtaatc aatgtcatac
      661 gctatactat catttccgtc tctaggttct ctctctatct cgctgtaacg ttgtgccaag
      721 atattaaatt catattctgt gattactact gttataggtt cataatcctg gatttcataa
      781 gtcgattttg cccatgtgaa tccctgtatc atcgctgatt cataacaatg gattagttga
      841 ttataaagat ttacaaattg cttaatttcc cctggaacct ccggtaaatg actgaagttt
      901 tctatgccag aaatattaaa tacggactta atttcttcga atatatcgtt caacttttct
      961 aagttattat ataatttttc aacaaataac ccttttgctt gtttgcctga gtataactca
     1021 atagcctctt caatgttctg ttccatcgta tgtgctttac gataataacg aatggttccg
     1081 aacggttttt caggaccatt aagacgattt gtgcgtgaaa aagcctgaat tatgttctga
     1141 tattcaagtg acttatctaa gtatagggtg ttgacccatt ttgaatcgta acccgttaag
     1201 agctgattaa caactataac aagatctaat tgggttgctt catctactgt tttgtaagga
     1261 ttcttatgcg ctgtgttttg caatagtttt gtacacaaga ttgcaaagaa aagtacacaa
     1321 tgttgccact tctggttcca tgagttttat ttgtgttgat gggacaacca atcagctatt
     1381 tacgaccctt gaagatcgca gactttacaa actaactcaa tacttcctgc gctttcctag
     1441 aaaggcccgt ttaaaggtca agtacctagt gatggacatg aatgccagct attgtcagtt
     1501 gcttaaaacg gtctttccaa atgcggagat cgtaactgac cgctttcata ttgtccaaca
     1561 catcaatcgc agcttcaatc aattacgtgt ccagatcatg aatcgttttc gaacctcaca
     1621 ctctgaaaat cagaagaagt accgtcggct caagcgatat tggaaactct tactcaaaga
     1681 ttccacgaca tgggaggcac tcaaacggca ttatcatcga ctgtttaaac gaccaattag
     1741 tcaaacagaa atcgttaatg agctgttaag ctacaacgaa gaattgcgaa ctgcgtatca
     1801 tttttgtcaa ttattacgct attatttcgt taaacgagat acggtaggct ttcaagaaac
     1861 ccttaaaaca atttcatcta cgttacctca gtcgtttaaa aagaaattca cgatcttcca
     1921 tcgctaccaa tcgggtattt caaatgcctt taaggtgtcc cactcaaacg gcgtcactga
     1981 ggggctgtat aacaagatca agctgattaa acgaattgcg tttggttatc gcaactttta
     2041 taactttcgg gcacggatct atttacaaca aggtctgatc tttgaaaact aaaaaaatat
     2101 gcggcgcaca gaacgcgccg catatcggac taa
//
LOCUS       DAYYHX010000278         2130 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000278 DAYYHX010000000
VERSION     DAYYHX010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.281"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..252)
                     /locus_tag="ACHA6Y_002783"
     CDS             complement(<1..252)
                     /locus_tag="ACHA6Y_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8988804.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNE"
     gene            complement(587..817)
                     /locus_tag="ACHA6Y_002784"
     CDS             complement(587..817)
                     /locus_tag="ACHA6Y_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8988805.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(832..1782)
                     /locus_tag="ACHA6Y_002785"
     CDS             complement(832..1782)
                     /locus_tag="ACHA6Y_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM8988806.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1779..>2130)
                     /locus_tag="ACHA6Y_002786"
     CDS             complement(1779..>2130)
                     /locus_tag="ACHA6Y_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8988807.1"
                     /translation="LMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITP
                     AATAYLYANSLKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLI
                     SPKQKFLRKKERKL"
BASE COUNT          653 a          402 c          270 g          805 t
ORIGIN      
        1 ttcattgaag ttctctctcc aacttttctc tatatctata ttttctaagt ttaagaggta
       61 tttggtaaac tcttcattat acatattaat aagcgcatta atatgcggct gaataatttc
      121 ttcaaataac gcttccttag aagtaaaatg tttataaaaa gcacccgtag ttacgcctgc
      181 ggtcaagcac agttgcctta tcttagtttg ctgaaatcct actttttcaa aatggcttag
      241 tccactttct attaattttt ggtgagttgt aatataatct ctggtttcta tgggtacttc
      301 ctcctaataa gatgaattaa ctatattatc ttacacagga cttgaagttt caatcgttaa
      361 cgtctattac tgaactcatt ccttagagct aaactaatga gaaagagcaa aataactgat
      421 acaaaaagca atcctacaag aattaataga ctcatctaaa tcattcacct tttctaaaat
      481 aatccgagaa agatttaaaa aaattatatc cagctattag gataagtcct atcataatca
      541 ctccaatagc cattttctta tcctccctaa aaataaagcc ttttacttaa aacctttttt
      601 tgtaatcgtc aataaatttt ttccctttat cagttaattg aaatcctata tctaaatagt
      661 cctcatatct aagtttagtc agcatctcac ttacagatgc tggactaaca cttagaactt
      721 ttgctaaatc attatttttt aatttctttt ttctttttga taattcctga attgctagta
      781 aataaattag tatagtagtt gttttaattt gtaccatacc taccctccaa attatttcga
      841 aaggccttca gcaattttat ccaggttcca tttcatcatc gcatagtaac tatcaccatc
      901 ttgtcctttt tctgcaattg aatcagtaaa aatcgttgaa tagataggaa tattggtatc
      961 ttttgatact gttttcattg gtctatcgtc cacactgctt tctacgaata aggagggaac
     1021 ttttgttgtg cgtaattttt caactaagtg ttttatttga tctggtgttc cttcttcttc
     1081 agtgttgatt tcccaaatgt aagcagaagg cacattatac gctttcgaga aatatttaaa
     1141 gcatccttca cttgtaacaa tcattttttt atcattcgga attgaagcaa atttagattt
     1201 agcttctttg tctagagaat ccaatttttc aatatactta tctagatttt ctttatagaa
     1261 ttttttatta tcaggatctt tttccgctaa ctgtttttca atatttttag cgtaaataat
     1321 accgttttct aaatttaacc aagcatgggg atcttccttc cctttctcac tctgaccctc
     1381 taagtaaata acatctatgc catcacttgc tgcaaaatag tctttgtttt cctctttgtt
     1441 cgcatttttt actaattttg taaaccaagc atttcctcca gtttccaagt taacaccgtt
     1501 ataaaaaatc aaatctgctt ttgaagtttt ttgaacatct tcaggcaaag gttcatattc
     1561 gtggggatct ttcccaatag gtacgatact gtgtaaatca attttatctt ttgctatatt
     1621 ttcagtaata tctgctaaaa tcgaattagt aactactacc tttaattttt cgttcgaagc
     1681 cactgtatct ttgtctttac tatttgttga acatgccccg aatataaaaa gagcaatcaa
     1741 aatccccaga cttcccagta ctgctttcca ttttctcatc ataactttct ctccttcttt
     1801 cttaaaaatt tttgttttgg tgagattaga aaactaatta caaataagaa tgccgctgtt
     1861 aaaacaatac ttgatcctgc tgctatgtta aaactataac caatgaacaa acctagcaat
     1921 gatgagcacg ctccgactgt cgccgaaata aatatcatag tttttaaact gtttgcatat
     1981 aaatatgctg tagcagctgg tgtaattaac attgcaacaa ttaatatagt tcccacactt
     2041 tgcatagctg ttactgctac taaagttaat agaatcatta atagataatg ataaaaactg
     2101 acattcatcc ccattgcttt tgccattaat
//
LOCUS       DAYYHX010000279         2125 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000279 DAYYHX010000000
VERSION     DAYYHX010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.282"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..159
                     /locus_tag="ACHA6Y_002787"
     CDS             <1..159
                     /locus_tag="ACHA6Y_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8988808.1"
                     /translation="RRNKFYRSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
     gene            242..859
                     /locus_tag="ACHA6Y_002788"
     CDS             242..859
                     /locus_tag="ACHA6Y_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988809.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
     gene            1023..1355
                     /locus_tag="ACHA6Y_002789"
     CDS             1023..1355
                     /locus_tag="ACHA6Y_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988810.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
     gene            1679..1840
                     /locus_tag="ACHA6Y_002790"
     CDS             1679..1840
                     /locus_tag="ACHA6Y_002790"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFM8988811.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            1872..2125
                     /locus_tag="ACHA6Y_002791"
                     /pseudo
     CDS             1872..2125
                     /locus_tag="ACHA6Y_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          758 a          272 c          429 g          666 t
ORIGIN      
        1 cgacggaata aattttatcg aagtctccgg actgcctcat ccacgattaa ggggatggag
       61 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaga tcaaggtatt aatgggaata ccagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga accattattt taaagtggga ggaaaattgt
      241 tttgtggata tttaaacaag aaatctttgc taaattattt agtttagatg gattaggttg
      301 gggaatctta ttttttattt ttttattttt aggatctatg tggatctggt ttgttgataa
      361 aaaaaattct tatgagaaag taatagtgaa aaaaatgaat cgaaatgaat tcgatgattt
      421 ttcaaaggaa catagagttg aattgtacga tagtcatgaa attaacagta cttctccatc
      481 ttattattgg gtatttggtt gcgaagaaaa accattggag attaaattgt tgaaaatgct
      541 tgaaaatcct ccagtcattg aagatttttc tgcaaaaaac gttgaaaaac agatgtttga
      601 acaggtacaa ataagaggtc aaaacaaaat ttcattagaa ccagatgaat atgcagcctt
      661 ttttttagaa acaaattcag gtacttcaat gcctgttttc aaagttaaat taaaaatgaa
      721 tggtcgttta tgcacttatg atcttgttgc aaatatgcgc tatggttcta ctggtgatag
      781 acgtactatt ttaaaatttg agcaaacctt tagaagtttt ttgaaaaata gagtatccca
      841 aatttttaat ttggattgaa aaaattaaaa atttggccaa aaatactaca gttttttaga
      901 gcaatcatct tatgatgatt gctctttttt tgttgttttt gtgtcattcg ttgttcaaat
      961 cctctattcg taaaataaat attttaaact agaaatatgc cttattttta ggaggaatca
     1021 aaatgaacaa tagagaaata ttggcaaaag aaatggcgga tttgaaagca aaagaagcga
     1081 agattagagc gaaacgaaaa cagattagac agaaagaaat gatttcttta gcgaaattat
     1141 attgtaaaaa ggaaggcttt aagacacctg ctgaagcatt agattttttg gaatcaaata
     1201 aaagaaatca ttcagtaaat gatgaggttt tagtatcgct aagaaaattt gcagatggat
     1261 tagtttggca agaaaatggt gcatattggc gtacgaatga tgttaagggt ttgactcaat
     1321 ttatggcaca atttcgttca gaaaatgtga actaaattga ggaggggttg aagtgaaaag
     1381 caatactttt cacttttggt tttttgcgga ataaaaattg tgaaaacaat gacgcaaaaa
     1441 gccaatggtt ttgaacgaaa aaaggacgta aaaaaggaat gcgaaggcag tcgtttttcg
     1501 ttcgcttttt cgtttgagag aaacggagtt tctcatcggc tgttaagccg atatagccct
     1561 cggcagagct tacagacttt acgcagtata agtctagtaa gttatacctt acatggaggt
     1621 ttttccgaat ttagcttgtg attctcgcaa taattttaac gaaaggaagt acacagttat
     1681 ggcctatatg gtggcacgaa cacaaaagca taaagttggg tcgttaaatg gacttcaaaa
     1741 tcatgtagat cgtaaaacga aaaatcatac gaataaagac attgatatta cgaaaagtgg
     1801 ttctgttgca aagttttcca aaaaatctat tttagtgtaa aattgagaaa aaagacagag
     1861 aggatagagt aatgaatcat tttaaaggca aacaattcaa aaaatgacgt cattattgtc
     1921 gctgttagtt actacctgcg ttacaatcta agctatcgtg aagttcagga attgttatat
     1981 gatcgtggaa taaatgtttg tcatactacg atttatcgtt gggtgcaaga gtacagcaaa
     2041 gtcctcgatt atctttggaa gaagaaaaat agacaatcct tctattcatg gaaaatggac
     2101 gaaacctata tcaaaattaa gggac
//
LOCUS       DAYYHX010000280         2124 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000280 DAYYHX010000000
VERSION     DAYYHX010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.283"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..677
                     /locus_tag="ACHA6Y_002792"
     CDS             75..677
                     /locus_tag="ACHA6Y_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HFM8988812.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            1032..1268
                     /locus_tag="ACHA6Y_002793"
     CDS             1032..1268
                     /locus_tag="ACHA6Y_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFM8988813.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            1265..1678
                     /locus_tag="ACHA6Y_002794"
     CDS             1265..1678
                     /locus_tag="ACHA6Y_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8988814.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
BASE COUNT          808 a          257 c          365 g          694 t
ORIGIN      
        1 taaagttata tgagtataag ttaatttact ggaggtagtg caatgctttt ttctgaaaga
       61 tttggtttta aaaaatggag gatatcatac tgagagatag attgacagac cctcttaaaa
      121 atagtttagc aaatgtgatt tatgatatat tacgagaggc tgataaagtt tatagtaatt
      181 ataatggaaa agtctcatgg gtaatatatt cttttttaat taaagagttt tttaaagaac
      241 agataaataa tagctatgag ttgggttatc attttactgt ttattatgaa gatattatca
      301 agaggttaga agacttggag tggtatgctt attatgattt attagaggtt tttgcaagtg
      361 ctaaaatctt ggataaaaag aaaagagagg aagtaaataa aattctggag tcggaaaatt
      421 ctggttacag attaagcgaa agcaatgaat ttattccaat cacagatgat atttctatta
      481 agtctataaa tgctgtatct gaatcaccat ttaattacgc aaaaaatcat atccataaat
      541 ctcttacata tataagcgag aaggaaaatc aagattataa tactgtagtg agagaagcaa
      601 ttagtgctgt cgaaagttgt cttatcgaat tttcaggatt accagctaat aaaaaaatac
      661 tttgggaact gctataaaag caattaaaga caaatcgaat aacaatgtcg atcttacatt
      721 tttaaaacca tttgagacaa tgtatggttt ggcgagtaat aatggaatac gacatgcaga
      781 aaatgaaaaa acaattgtct cagatttacc tgatgcagtt ttggtgttaa caacatgttc
      841 agccgtaatt aattatttgg gagtaaaaat agcatatgac tccaataaat catgaaaagt
      901 taaaattcat ccaagagcta cattataact attaatttcc aactctttaa aagtttagta
      961 attgaaggaa gtaataaggg tgattgaaca atacggatat acggtgtata cttatcgtag
     1021 gagttgatga tatgaatatt aaaaaacgca aaatcagaga agtaggtaat tcaatagttg
     1081 taactttgtc aaaagagagt ttattgcaaa aaggtttgaa acctggagat actattttta
     1141 ttgatgaaga taaaatgatg gaagctattg taaaagagga aagcaagata gatcttgaaa
     1201 ttgatatgta tgttaaccaa gctttatctg agtatgatgc agctttcaag gaattagttg
     1261 aaaaatgatc tatctaagtg aaaaagatat tgtaaaattg aatgttattc aaataaagaa
     1321 atattcaccg cgagaaacaa tcggagtcat tgacaaaaat gcgctgagta tggctgtaaa
     1381 tcaacctaaa cagcatattt ttggaaaaga tctgtatcct gataaattta gcaaagcagc
     1441 tgttctgcta ataaatctga tcaaaaagca tccatttcat aatgctaaca aacgtacagc
     1501 atttttagcc gcgtatgttt ttctcaagct aaatggctat tcactaaaaa tgaaaaatca
     1561 ggaagtagta gaatttgtag ttgaaattgc tacgtatcag ggtgattttg atgacttaaa
     1621 agaggcaaca acagatatac taaaaaagaa ttcaacaaag accgattcaa aaaagtagaa
     1681 gcaaactagg gattcagtta aatctgaaga tttagtttgt tctttatttt agtttacata
     1741 agatacatta tgcgaagttc attattttaa aagcttaaat aataaacttt tttataatac
     1801 caatttattt atgtaaacca atgaccttaa tcgcttaagc aagcatgtta tgtaatactc
     1861 tactctctcc aaaactaatg actatctatt ttcaaagaag ctagaatgat tatctgatca
     1921 aatgataatt tatatttaat tcctggtcta ctgctcttct tttaatcaat taatgtctaa
     1981 cgtactgtac acgataaagt aggaagactt attttcaact tcggattcat ttttttaatt
     2041 cggtgaaaat cactaaatta agagtttatc caagttcatt ttagctgaga atacgcttga
     2101 aaataatttt gatgaatttt cctg
//
LOCUS       DAYYHX010000281         2114 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000281 DAYYHX010000000
VERSION     DAYYHX010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.284"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..1004)
                     /gene="cysK"
                     /locus_tag="ACHA6Y_002795"
     CDS             complement(78..1004)
                     /gene="cysK"
                     /locus_tag="ACHA6Y_002795"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM8988815.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1144..2031)
                     /locus_tag="ACHA6Y_002796"
     CDS             complement(1144..2031)
                     /locus_tag="ACHA6Y_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8988816.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          584 a          473 c          356 g          701 t
ORIGIN      
        1 tcatcataaa tttttgtgtg ttcttttcat ctttaaaagt tttttacata aacctatctg
       61 gagaaaaacc aagtgcctta tatctcttgg taaagggctg tagataaata acgttcacca
      121 ttgtcgggaa ccacagctaa taccttcttg ccttttccta attcttttgc gacttttagc
      181 gcggcagcaa ttgcagcgcc agatgagatg cctactaaaa tcccttcttt tcgcccaact
      241 tctctagctg tttccatcgc ttcgtctcca ctaatagaaa ggactttatc atacacagaa
      301 gtatccagtg ttttaggaac aaaacctgta ccgattcctt ggattttgtg aggtcccgct
      361 tctttccctt ctaaaatagc agattctgct ggttctaccc cgattaattc gattttcgga
      421 tacacacgtt ttaattctcg gccagcgcca gtgatcgttc ctcctgtacc gatacctgca
      481 acaaacgcgt ccaagccatt tactccaaat gcttgatgta tctcaggacc agtcgtcttc
      541 tcatgcacaa gaggatttgc ttcattttca aattgtaatg gtaaaaagta gccgttttct
      601 tttgccaaac gttctgcttc tctgattgat ccagaaatac catccgcacc aggagtcaaa
      661 atcaactctg cgccgtaccc tttcatcaat aaacgtcttt cgatactcat cgtctcaggc
      721 atcacgatga ttactttata tccttttgca actcctacca tcgacaatcc gattcctgta
      781 tttccagaag taggttcaat gatagtatca cctggtttca gtagaccatc gtgttctgct
      841 ttttctatca tacttaaagc aatacgatct tttacacttc ctcctggatt aaaaaactca
      901 agctttacaa aaacatctgc tgaatcttct ggtacgattt tatttaattt gacgattggc
      961 gttttgccaa tcaattcggt gactgagtta tagatagctg tcatcttcaa ttcctccttg
     1021 tctttgctct tcttacttat cataatacaa tctgttcatt tatggcgagt gattcgtctt
     1081 tgactagcaa aaaaaagaca aagatatacc atatatggta atttctttgc ctttttatct
     1141 aaatcaaacg tttagttctt gaccaactaa taaagaataa ctggacatag acatgccatt
     1201 tttcgccatt agatcatcaa cagacatgcc aaatttttgt gcaatatccc atagcgtatc
     1261 acctgacacg actgtatatg tctgaccatt atttgtattt acatctgttg ttgaagatga
     1321 tgacgttgaa gtcgtcgtag tagtcgttgt tgttgtactt gtggttgttg tagccgaacc
     1381 tgttgatggc gtatcaaaac gggttaaatt ataagtatta atcaatgagt tcaatttggc
     1441 cccataattt ggatcggtag cataacgacc tgtcaagtac gctgttgcat cataaaaact
     1501 tgttgtattg cttttccaca cgccagcata atgataatcg cccgtggaaa aactggttga
     1561 acgaagaaca ttcgcatggt cttggaagga ttcccaataa gaagaatatt ttcggaatgg
     1621 ctcattcatc gttacccatt gtccgtttac atattctttg gttgccatat aaacgacctg
     1681 tccgttatag cttcctttca caccgaatag attataattt ggcgcactag ctaatccgga
     1741 agtgccatat gcactttcca acaacgcttg agcgatcatg actgaagcat acaagtcatt
     1801 acttgcagca acttctgacg cagaccagcc gatttgatcg ataaacgctt gaggatcagc
     1861 gactgtttga gatccttgtt catctgcatg aacatgttgc ggtaccgctg taaccatcat
     1921 cggcccaact aaaagactcg ttccaattaa aggagcaatc gcttttttat tgatcaccat
     1981 ttttctttta ggtctatggt gccgggcact gcgggtcttc agctcttgca ttcaaattcc
     2041 tcctcaaaat aaaaaaactt aataaaaagt tctcttctta aaacaaactt aattaattaa
     2101 attatacttt taat
//
LOCUS       DAYYHX010000282         2106 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000282 DAYYHX010000000
VERSION     DAYYHX010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.285"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(589..789)
                     /locus_tag="ACHA6Y_002797"
     CDS             complement(589..789)
                     /locus_tag="ACHA6Y_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8988817.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(923..1531)
                     /locus_tag="ACHA6Y_002798"
     CDS             complement(923..1531)
                     /locus_tag="ACHA6Y_002798"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM8988818.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(1616..2086)
                     /locus_tag="ACHA6Y_002799"
     CDS             complement(1616..2086)
                     /locus_tag="ACHA6Y_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM8988819.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
BASE COUNT          673 a          428 c          310 g          695 t
ORIGIN      
        1 gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat gtattttttc
       61 cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg atcgcacata
      121 cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg atttcacgac
      181 aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt tatttccagc
      241 tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca tcttttaatg
      301 gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag aattgacatg
      361 gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat ccaaaaactg
      421 aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata attagttagg
      481 acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt cataaataga
      541 caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt ataatttagt
      601 cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta cttcttgacc
      661 ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa atacgtcatc
      721 tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt taaccgttcc
      781 gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc aagaacatta
      841 tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat tatttttaag
      901 actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt acttcatccc
      961 aattaataac attccagaat gctgcaatat aatctggtcg gacattttta tatttcaaat
     1021 aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc atcaatggag
     1081 aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca agccaagccc
     1141 atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa gaagaaaaat
     1201 caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca ccagcatttg
     1261 gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg ttacgtacag
     1321 ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct attgtttttt
     1381 cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa gtattgtgat
     1441 gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat gcgtcatatg
     1501 cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat gttttcttaa
     1561 taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt tatgcctagt
     1621 cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta aaaataaaca
     1681 acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt ttcgccacat
     1741 actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc aatccgtaag
     1801 aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta aaattcggtg
     1861 attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca gaacaaaacc
     1921 acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg agagcaaaac
     1981 taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt atctcccaat
     2041 atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc caacctacct
     2101 tattaa
//
LOCUS       DAYYHX010000283         2076 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000283 DAYYHX010000000
VERSION     DAYYHX010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.286"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..425)
                     /locus_tag="ACHA6Y_002800"
     CDS             complement(<1..425)
                     /locus_tag="ACHA6Y_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8988820.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="ACHA6Y_002801"
     CDS             complement(534..1565)
                     /locus_tag="ACHA6Y_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8988821.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          642 a          336 c          360 g          738 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agatttattc
     1981 tatggtagga gcaattatat tgagaattaa tcctttttta tataagttgt ttgcttattg
     2041 atggattgga taaattaaac catcacaacg agtgga
//
LOCUS       DAYYHX010000284         2056 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000284 DAYYHX010000000
VERSION     DAYYHX010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.287"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            493..714
                     /locus_tag="ACHA6Y_002802"
     CDS             493..714
                     /locus_tag="ACHA6Y_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988822.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="ACHA6Y_002803"
     CDS             715..972
                     /locus_tag="ACHA6Y_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988823.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="ACHA6Y_002804"
     CDS             complement(1108..1710)
                     /locus_tag="ACHA6Y_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8988824.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="ACHA6Y_002805"
     CDS             complement(1777..1932)
                     /locus_tag="ACHA6Y_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFM8988825.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          462 c          314 g          602 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggtggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaag cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatta ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DAYYHX010000285         2025 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000285 DAYYHX010000000
VERSION     DAYYHX010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.288"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..1347
                     /locus_tag="ACHA6Y_002806"
     CDS             121..1347
                     /locus_tag="ACHA6Y_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8988826.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(1455..1925)
                     /locus_tag="ACHA6Y_002807"
     CDS             complement(1455..1925)
                     /locus_tag="ACHA6Y_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM8988827.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT          756 a          312 c          395 g          562 t
ORIGIN      
        1 aaaaataata atcgttacac aaaaatgttt tttaaagttt tcttatgatt aaaaagtgaa
       61 tttgctgatt gaacagttat atgagacaat attttagaaa atgtgaaaca gaggtgtatt
      121 atgaagcgat ggcaaaaagt tctattaacc ttgttagggg tattcgtttt gctgattgcc
      181 ggattatcag catacggaat cagatttatg ggagaagcga gccaaacagt aaacaaaatt
      241 tctaaaaact ctaatcgaat atcctccaaa cgttctgaga gagtaagcat tgatgacaaa
      301 gaaccttttt cggttttgct attaggttta gataccggtg gattaggaag aacggaacaa
      361 ggacgttctg atacgatgat ggtagtaaca gttaatccga aacaaaaaaa atcaacgatc
      421 atcagtttgg accgagatat ttatacgaat atcgtaggtt atggaacggt agataaatta
      481 aatcatgcct acgcatttgg tggtgtcgaa atggccatgg attcaattga gcaactgctc
      541 gatattccta ttgatcatta tgtaacgatc aatttggatg gcatggaaga tttgatcaat
      601 gcggtcggtg gcatcaaagt gaataataaa attgacttta ctttggatgg tgtccatgta
      661 ccagtaggtg aacaaacatt gaatggagaa aaaggtttag cctattctcg aatgcgacac
      721 gaagatccag aaggcgatat cggtcgacaa cgtcgtcagc gggaagtagt gacgaaaatc
      781 gtcaataaag ttttaagttt agatggcgta agcaattata gaaaaatttt aaaagcagtt
      841 gagaacaatg tgacgacgga cttagattgg gatgacatgc ttgatgtagc gacgaactac
      901 accccggcgt ttgaaacaat caaacaagac cagttgcaag gcgaaggaca aatgatcaat
      961 gatatctact atcaaatttt aggaaaaaat gatcttcttt cgatccaaaa tgaactgaag
     1021 aaacaattaa atataaaaac aagcgataca ttacctaatt tgaaaaatga gaatgcttca
     1081 atgatgttct ttgatgattc ggaagatggc gattcgacaa acggtgatgg atcgaatagt
     1141 gaagggtaca ccgattattc ttcctattca gaaaacacat atgatcagta ccaagatccg
     1201 aatacctatt atcagcaaac agaagataca agtcagtact atacaccaag tcaagatact
     1261 caaagttatg atccttacgc tggacaggaa caaaatggtt atggttatga ccagaataat
     1321 gagtatggaa caacaagtgg gtactagaaa aaaatagttt ggttaataaa gagtgccacc
     1381 taaattagac aaaaaatcta acttaggtgg cactttttct atgtgttaaa ttacaggacg
     1441 tctcttttta gcgcttagtt taaaaaataa attgaataat tcaatagagt tgcgaatagt
     1501 acccataaat aataaggaat catgagccac attgccaggc gatgatgcag ccacaactga
     1561 tacataagca ctgacaagaa aataactaaa agtgtaatat cgattgctgc aatgaaaata
     1621 tttcttaaat taaaaaatag agcagaccat ataaaattaa aaatgaactg aatcacaaaa
     1681 agaccgacaa gattgtttcg ctgttttttg ttgttgtgat taagtaacaa ataaagagaa
     1741 atacccatca atatatacag taaggtccac atcggcccaa atatccagct aggtggtgca
     1801 aatggtggaa gttgcaaaga atagtaatat tttccgggat ttccgctaag caaccctgat
     1861 aaaaagccta atatgacaat tcctataaca caaacccaaa aacgaatatc ttttatcatc
     1921 ttcattttac taactccttt ctataatatt agtttaccat gaaggctcac agcaagacct
     1981 gtatttagtt tgtgaagtaa gaaaaaaatt gataattgtt gttat
//
LOCUS       DAYYHX010000286         2007 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000286 DAYYHX010000000
VERSION     DAYYHX010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.289"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..688
                     /locus_tag="ACHA6Y_002808"
     CDS             50..688
                     /locus_tag="ACHA6Y_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8988828.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            1065..1976
                     /locus_tag="ACHA6Y_002809"
     CDS             1065..1976
                     /locus_tag="ACHA6Y_002809"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8988829.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT          781 a          294 c          404 g          528 t
ORIGIN      
        1 gtctacagaa aagaaaagtc agttattgct gatgaatgga ggttaacaat tggagccgtt
       61 gatagtgata aaaggtgcga aaaaaatcat tcgaaacaaa acgattcttc atttggatcg
      121 tttcactgcg gatgctgggg attttgtgac tgtagttgga gagagcggag cagggaaaag
      181 tactttattg aatatattag gattgaatga tcaattcagt tcagggcaat tttatttatt
      241 taatcaagaa acaaaacatt taactggaag agagaagcgc ttgataaaaa gaaatgccct
      301 ctcttttctt tttcaagatt ttgggttgat tgaatatgaa acaatcgatt ttaatttaga
      361 tattggattg aagttttcga atttaaatgt ttcagataag cgaacagcaa agtatgaggc
      421 attgaaaaaa gtaaatataa acaagaagct aagtacgcct atctacacat tatctggtgg
      481 tgagaagcaa agaatcgcct gtgcgagagt cttattgaag aatagcaaaa tcattttagc
      541 cgatgaacca actggatcat tagataaacg aaataaggag caagtcgttg aaatcttatc
      601 gcaacaggct tcccaaggta cgttagttat cgtggtcacg catgatccat atctgaccaa
      661 aataggaaac aagaagatca tgctttagaa aacaaatgaa aaaacaaact ttttctaata
      721 aaaacaacaa aaaaatgtct ggaaaatcga acataaaagt gtctgatttg aatatcccaa
      781 cagcggaaga agaaaaacaa aatcgataac atttgttcta agaattagtt acgttaaata
      841 cggaacgcaa gatagacttg aaaaacggta acaaaagaag tagtatatta aggtgtgatg
      901 tgctacttga catttattct gctgagcgaa ctttctggat tgtgtagcct tatatctatt
      961 caggacgtat cgagcagatt agtaaaggtg ttcttgatga ttttcatctt gaacaccttt
     1021 gtttttagca attcgcaaaa aacgaaaagt aggagaattg ataaatgaaa aaaagatttt
     1081 tagcacttgc catcgtacta ggcactggct tattgtcagg ctgtacgaat gccggtgaaa
     1141 aaacagccgt tagttataaa ggcggtacaa tcagcgaaca agaagtaatg gatagcttga
     1201 aaaaaatgca aggagcagat tccgctgtgc aacagttgat cgtttatcaa gtatttgaag
     1261 ataaatatgg agatgacgta tcaaccaaag aaatcgattc ccaatatgat caaacaaaga
     1321 aacaattggg tgattcattt gattcacaat tgaaatctgc aggttacaca gaacaaacat
     1381 tcaaagacag tatcaaacaa agtctagcct ttcaagaagg tctaaagaaa catatcaaat
     1441 tgaccgatga agatttaaaa acggcttggg aatctttcca tccagaagtg gaagctcaaa
     1501 ttatccaagt agcaagtgaa gatgatgcaa aagatgtgaa gaaagctgct gataagggag
     1561 acgatttctc taaattagcc aaagacaagt caactgatac tacgacaaaa gaagatggcg
     1621 gaaaagtgaa atttgattcc actactacaa cggttccggc agaagtcaaa gaagcagcct
     1681 ttaagttgaa agatggtcaa gtttctgatg tgatcacttc taccaatgct tcaacttata
     1741 ctacggaata ttatgtggtt aaaatggtga aaaaccaaaa caaaggcaac gacatggata
     1801 aatacaaaaa agaattgaaa gaaattgcaa cagacacaaa attaagtgac agtaccttcc
     1861 agaataaagt aattggcgaa gtattgaaag acgctaatgt aaaaatcaaa gacaaagact
     1921 ttgaaaatgt tttgagtaca ttcacttcag attctagtac agcaagtagt aaataagcgg
     1981 tataaataaa aaatatgaac tatttaa
//
LOCUS       DAYYHX010000287         2004 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000287 DAYYHX010000000
VERSION     DAYYHX010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.290"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACHA6Y_002810"
     CDS             <1..378
                     /locus_tag="ACHA6Y_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM8988830.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACHA6Y_002811"
     CDS             426..1826
                     /locus_tag="ACHA6Y_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8988831.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          340 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttac
//
LOCUS       DAYYHX010000288         1992 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000288 DAYYHX010000000
VERSION     DAYYHX010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.291"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..363
                     /locus_tag="ACHA6Y_002812"
     CDS             <1..363
                     /locus_tag="ACHA6Y_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFM8988832.1"
                     /translation="QAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKIKKMLLG
                     KTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQFIAADYD
                     ADLADQFINGNNFMPTTN"
     gene            complement(509..985)
                     /locus_tag="ACHA6Y_002813"
     CDS             complement(509..985)
                     /locus_tag="ACHA6Y_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8988833.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(945..1301)
                     /locus_tag="ACHA6Y_002814"
     CDS             complement(945..1301)
                     /locus_tag="ACHA6Y_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988834.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(1279..1464)
                     /locus_tag="ACHA6Y_002815"
     CDS             complement(1279..1464)
                     /locus_tag="ACHA6Y_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988835.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(1465..1737)
                     /locus_tag="ACHA6Y_002816"
     CDS             complement(1465..1737)
                     /locus_tag="ACHA6Y_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFM8988836.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(1734..1934)
                     /locus_tag="ACHA6Y_002817"
     CDS             complement(1734..1934)
                     /locus_tag="ACHA6Y_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988837.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
BASE COUNT          592 a          380 c          287 g          733 t
ORIGIN      
        1 caagctaaat ctgagcaatt ggaacaaatt gctggagttg gttatagaaa agctctagaa
       61 tttctaatta aagattatgc aatatctaaa aattcagagg atgaagataa aataaaaaaa
      121 atgttattag gtaaaacaat tgaaaattat ttgaatgatt ttcctaaact ccaaagatta
      181 gccaaagctg ctacttggat tggtaatgat gaaacgcact atgttcgtag acatactaat
      241 aaagatgttc atgatatgga tgcatttatt aaatcagcag cgcaatttat cgcggctgat
      301 tatgatgctg atttagctga tcaatttata aatggaaata actttatgcc aactactaat
      361 taaaagaata aaagagccac agataccaat taataaaacg gttgggatat cttggctctt
      421 tcatattttt ttgacactaa tagaatatca tgctgctaac aatgtttaca tagctacaat
      481 gaccgaacat ttagtgaaca gtgtaatttt agaataaaga gagttgttct ttgtacttct
      541 cgctattgta aaaatcaatt tcgttttctt ttttcaatcg gtcagcttgt ctgtgttcat
      601 actcatctaa aaagttcaat gtctttctaa tttctgcatg tcgctgtctg acatacgatg
      661 agctataccc aacagattca gcaatatctt ctaagctcat ttgatcaaca tatttcatct
      721 taacaatttg attatcgata ccagaaaagc tatctatcag caataacatt tcttcttttt
      781 gctcgatcaa caattccaat tcattttcta tcttttggat attctcttct aaagaagaag
      841 ttctcgaatt cttttcgatg cggacgtttg ctaaatcacc acagacccat cgattcaatt
      901 caagcttact tttattaaga ttccacttta agtaaagaat ctgctcatct agttcttggt
      961 aatcttttaa ccaatgaaat ctcacaaacg ccacccctta tggtagaata gtattgcgct
     1021 gctatctcga aaggggtagc ttttttattt tttcttagcc gttccattct ggattgcctt
     1081 ttgttccaaa cgtcgttttt tcttcttaat ctttgatttt gtttttccca tacaattctc
     1141 ctttccgaaa acacattttg ttcgattttt aatactaaat aaaagttggc cagaattttt
     1201 cgttcgtctt tactcatctt tttttgaata agattactag cataaaaata gttagtacca
     1261 cgaaaatgaa caggtaaatc aattacgttc accttcttca tgccagttat cagccactag
     1321 aatctttgcg ttagtgccgc caccgttttc ttttttgtat ttttcaaatg ctttataagc
     1381 agctgttttt gatttataga tttttccgga accgactggg aacatccatg tgccctctgt
     1441 gatagctaag atgtatactt tcatttaatt caccccttta atccccaaca ctacatatcc
     1501 ggacttctgc atgtaatcag taatataagt gatctctgct ttgtatgagt caccagtgta
     1561 actaaatgtt ggtctcgtta gtccaggtaa ttcatgttta atgtattttt tatattcacg
     1621 tagtattaac tgatcaccaa ctttgaagtc acgatcattc ttgcggatct cgaattgttt
     1681 gcgtccgctc gtcactgctt caaaatattc tggtagtatt tttagttcgt ggatcattct
     1741 gtttcctcct tcaaaattcg ctcatcatca aaaagtgaat ctccacaaaa tccttctccc
     1801 aaatatttat cgttcatgta taagtcaacc aaatatttgt gatctgcttc tcgatattca
     1861 ttaatattaa cgatctttat tttgtaaaca ttaccttcgt cgctcactat cttgaattgt
     1921 tctctgactt tcaccaccgt tcctcctctt tttgaaaaaa ttttagtgtt tttgtaagac
     1981 cccctaaatc ag
//
LOCUS       DAYYHX010000289         1989 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000289 DAYYHX010000000
VERSION     DAYYHX010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.292"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..525
                     /locus_tag="ACHA6Y_002818"
     CDS             <1..525
                     /locus_tag="ACHA6Y_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8988838.1"
                     /translation="ISTETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMG
                     LDETNSIEQPNLDRIKTARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQE
                     DDSDFLFLKSILEDSFEYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNN
                     LKREITARLISKLQ"
     gene            580..1746
                     /locus_tag="ACHA6Y_002819"
     CDS             580..1746
                     /locus_tag="ACHA6Y_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8988839.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
BASE COUNT          751 a          323 c          323 g          592 t
ORIGIN      
        1 attagtacag aaactggtat taactataca actcttacga actattaccg tggtgagcga
       61 aatcctagaa atactgaaac atggaatatc cttgctgatt atttccatgt tcctgtaagt
      121 tatttgatgg gattagacga aacaaattcc atagaacaac ctaatcttga tagaataaaa
      181 actgctcgtt tagaaaaaaa ggacaaaata gtcaagaata ttgccagtct tgactcagat
      241 tattttactg aattatctaa tctatacttt tccatgactt ctttacttga agtcaaactt
      301 caagaagatg actccgactt tcttttttta aaaagtattc tagaggactc ctttgaatac
      361 acgattctat ttgccaaaca aaagaagttt attaataaag ctttaaactt aaatatcgat
      421 gaaagaaaag agttagaatc tgagttattt ggcaacttaa cccaagtaca ggttaacaat
      481 ctaaaaagag agataacagc tcgattaata tcaaaacttc aataaaacta attattcaat
      541 cagccaactc gccaagttat cagattggag agattagtta tgtcaataca aaagtacaca
      601 acaaaaagcg gtgcacgata tagtgtccgc ttgtatgttc gtaaagatga aaatggaaag
      661 caagaatact atgtaaaaaa agggtttaaa actgaaaaag aagccaagtt acacgaagca
      721 agaaaaaagg tcgagattga agataccggc tatcataagc cttctaatga cttgtttata
      781 acagtgtatg aagaatggtt gccttcttat aggaatcgtg tacaagaaac gacatatcaa
      841 cgtacaaaag accttttccg cttgcatata ctaccaaaat tcgggaataa gtccatttct
      901 aagattactc ctgttcactg tcagaatgca gtcaatgaat gggctttaaa attcaagaat
      961 atcaaacagc ttaagtctta cagctctcaa atatttgagt atgccatttt tgcagagcta
     1021 ttacaccgaa atcctatggc aaatacaaat ctaccaaagc gggaaaagaa agaaaatgaa
     1081 aattactttt cattagaaga actaaaagct ttttgggata ttctcaataa agaagagcct
     1141 ttaaaacata ttttaatctt ccagcttttg attactactg gtatacgtaa aggagagcta
     1201 gctgctcttc attggtctga catttatttt gaagaacagc taatgtatgt tagaaagagc
     1261 tatgcaacta ttcgaaatga agagaagaat gcaagaagaa aaactgtcag gattcaaaaa
     1321 aatacaaaaa atatatcttc cgaacgaata ttaccaattg atgcacaaac tattgaaatg
     1381 ttaaaaatct ggaaaaaaga acaagctcga gagcttttgc agtttggcat aaatacaaat
     1441 acaaaaaatc aacttatctt tacctatgta aatgcagacg gtgacattaa tcaacctttg
     1501 catgcggact attcaaataa tatattgaaa cgtttagaga agaaatataa atttaagcac
     1561 gttactattc atggattccg gcacactcac gctactcttc tactagaagg tggagcaagt
     1621 atcaaagaaa cacaagatcg attgggtcat aaaaatgctg aaactaccct taacacttat
     1681 tctcatgtta ctgaaaaagc tcaacgaaat gcgttagaaa aattcattgc ttacactggt
     1741 ttataatatt gtcattacct ttctcattac cagaacgaaa aaaagacacc ttctcgcaat
     1801 gagaaagtgt ctgaaatcgt tggtataaca acgattaacg ttttgaaaac tgtgaagctt
     1861 tacgtgcttt cttaagacct ggttttttct tcgtatcatt gaataagttt gaaaagcctt
     1921 gatttataaa ggtttttccg taaatatatt ttgttatatt tgattaaatt tgaaatgttt
     1981 gtgttctaa
//
LOCUS       DAYYHX010000290         1968 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000290 DAYYHX010000000
VERSION     DAYYHX010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.293"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACHA6Y_002820"
     CDS             <1..610
                     /locus_tag="ACHA6Y_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8988840.1"
                     /translation="HAKVIIETLRLENKLIYGFFDDKQNKMQDLKYLGKINELNSWAD
                     SKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIAVHPSAIVAKEISIGYGTVIFANA
                     VINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPGVCLAGGVHVGVGTQIGIGSQCIQ
                     MKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKKEGINFEF"
     gene            561..1742
                     /locus_tag="ACHA6Y_002821"
     CDS             561..1742
                     /locus_tag="ACHA6Y_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8988841.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            1755..>1968
                     /locus_tag="ACHA6Y_002822"
     CDS             1755..>1968
                     /locus_tag="ACHA6Y_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8988842.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSP"
BASE COUNT          703 a          259 c          407 g          599 t
ORIGIN      
        1 acacgcaaaa gtcattattg aaaccttaag attagaaaat aagttaatat atggattttt
       61 tgatgataag caaaataaaa tgcaagatct gaaatatctt gggaaaataa acgaattgaa
      121 tagctgggcg gatagcaaaa aaaaaatcta taaatttatt gtagctattg gtgactgtgt
      181 agtaagaaaa aagattataa aaaaattgaa tatgccaagt gagcaatatg gaatagctgt
      241 tcatccaagt gctatagtgg caaaagaaat ttcaattggt tatggaactg ttatttttgc
      301 gaatgctgta attaacccag atgctgtaat tggagagcat gctataataa acacaggtag
      361 cattgttgag catgattgta gaattaataa ctatgttcat ttatctccag gtgtatgctt
      421 agcgggtgga gtacatgtag gtgtaggaac tcaaatagga attggttctc aatgtatcca
      481 gatgaagaaa attggatctt ggtgtatgat aggcgcaggg agcactattg ttaaggatat
      541 tccctcacat tccttagctt atggcaatcc agctaaaatt aagaaagaag gaattaattt
      601 tgaattctag aattttatta tcaagtcctc atatgggagg aacagaagaa aaatatgttg
      661 ctaaggcttt taaagataat tggatagcgc cattaggtga aaatgtggat aaatttgaag
      721 aaagtattac aaaatatact ggttctaagg gcggagcttc tgcgttaagt tcaggtacag
      781 ctgccataca tcttgcctta agactgctta atgttggaaa aaacgatatt gtctttgctc
      841 cttcatttac ttttatagct acagtaaatc ctattttata tcaaggggct gtaccagtgt
      901 tcattgactc agaacctaat acatggaata tgagccctgt tgcactagag agcgcattag
      961 aagaagctga aagaaacaac caattaccta aggcaatcat cgtagtgaat ttatatggtc
     1021 aaatggctga ttatgaacga attttagcta ttgcaaacca atttaatgtt ccagtgatag
     1081 aagatgcagc ggaatcgtta ggttctactt ataagggaat ttcgtcaggc ttatttggga
     1141 caattgggat ctattcattt aacggtaata agatcatcac aacctcaggt ggaggagctc
     1201 tagtgtctag tgataaaaaa tatgttgagc attcaagatt tttagcaaca caagcaagag
     1261 atcaagcacc gtattatcaa catagcgaaa taggttataa ttaccgtcta agcaatattt
     1321 ctgccggtat cggacgtgga caaatggaag tattgaatga tagagtagct aaacgaagag
     1381 aaattttcaa tcgatataag gaagcttttt ctggaattga tagaattgaa atgatgccag
     1441 aagtggatgg catggtgcca aatcgttggt tgtctacttt aacgatcaat gattataatt
     1501 ctattgatca agtagttaac gtaataaata aactaaatga aaaaaacatt gaaacaagag
     1561 ctttgtggaa accactacat ttgcaaccag tatttcaagg ggtaaaaatg tattaccatg
     1621 ataataatct tagtgtgtca gaaaagttat ttaatagcgg cttatgctta ccttctggaa
     1681 gtaatatgac agatagtgaa caagaatttg ttattgatga gtttaaaaaa atattcaagt
     1741 aggtgtgata ttttatgaac ataggaatta ttggtttagg ttccatgggg aaaagaagac
     1801 ttagactcat tcaagaacac tttaatgata ttttactatg tggtgttgat actaatgagg
     1861 cgagacgaaa ggaagttgaa aaggaatttg gaataccaac ttataatagt ctaacagagg
     1921 caaaaaattt gtttgaaata aattcagttt ttgtttgtac atccccta
//
LOCUS       DAYYHX010000291         1963 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000291 DAYYHX010000000
VERSION     DAYYHX010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.294"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHA6Y_002823"
     CDS             64..972
                     /locus_tag="ACHA6Y_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8988843.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1592
                     /locus_tag="ACHA6Y_002824"
     CDS             972..1592
                     /locus_tag="ACHA6Y_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988844.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1596..>1963
                     /locus_tag="ACHA6Y_002825"
     CDS             1596..>1963
                     /locus_tag="ACHA6Y_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988845.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTMVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTT"
BASE COUNT          697 a          301 c          443 g          522 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag tcatatgtag
     1501 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
     1561 attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta aaaataaaac
     1621 aaagcaatgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
     1681 tggtgaaggg agataaaggc gatcctggcc aagcagcaac aattaccata ggaaccgttt
     1741 ctagtggttc aaccgcttct gttacgaatg taggaacatc atctgcagct agatttaatt
     1801 ttgttttacc aaagggagat aaaggtgatc ctggaataaa tgccacaact acagctgtag
     1861 ctacaactac agcaaatggg ttgatgtcgt ccacagataa aactaagttg gatggaattg
     1921 cagctggagc acagaaaaat ccaggtaatg ctacaactac gac
//
LOCUS       DAYYHX010000292         1949 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000292 DAYYHX010000000
VERSION     DAYYHX010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.295"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            270..836
                     /gene="amaP"
                     /locus_tag="ACHA6Y_002826"
     CDS             270..836
                     /gene="amaP"
                     /locus_tag="ACHA6Y_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8988846.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            849..1037
                     /locus_tag="ACHA6Y_002827"
     CDS             849..1037
                     /locus_tag="ACHA6Y_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8988847.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1050..1607
                     /locus_tag="ACHA6Y_002828"
     CDS             1050..1607
                     /locus_tag="ACHA6Y_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8988848.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1639..1881
                     /locus_tag="ACHA6Y_002829"
     CDS             1639..1881
                     /locus_tag="ACHA6Y_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8988849.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          713 a          304 c          404 g          528 t
ORIGIN      
        1 aaatagaatc ttgtaataat aactgagcat aatctttgca atttttaatc ttgaataata
       61 tcctttaagt atagccagca atcaaaagta aatagagaat aagtaagcaa ttgttatttc
      121 agtatcagac tggcaataaa acgccgttat gatccagcaa tgtttggtag cgctttattt
      181 actgatgcta tgctaactgt tcgctatcta tactgtgatt gtcggtagag ttatttacag
      241 ggtaaaaata agtttatagg aggtggaaaa tgaataaggg gttaaaaata atcggtgtta
      301 tcatgagttt actgctactt tcagtatttc tagcgattgc gttgatcaat gcgccgtata
      361 ttatgcctga acaattggaa cgatttcgct tctttacttt aacgaattac tacatgcaac
      421 aatatatttt ctgggcagct gtagtatttg ccgttcttgc tattatcatc ttgctagtcg
      481 ttcttttcta tccaaagtca agaggaactt tcgttatgaa acgggaagat ggcaaactaa
      541 caatcgacaa aaaagcgatc gaagggttgg tacgttccca tttacacgaa gaagagttca
      601 ttcattcacc taaagtacgg atacgttcaa cgaaaaatcg aatccatatc catgtcaacg
      661 gagacttgaa acgaacttct tcattagtag ggaaaacagg tgtattgatg caagatatcg
      721 aagaagaagt aactaaagta ctcggaacaa aagaaacggt caaagtggca gtcacataca
      781 gcggttatga agaacaagaa gatacaagag actacaagca ctctcgagtg gaataggagg
      841 gccaaataat gaaagaacta ttttcagcat acaagctccc tattatcttt gggggtcttg
      901 gactactgct agctgtttta tggttaacga ttggattttt caagacattg ttgatcataa
      961 tcatgacagc aattggcgca ggaatcggtt tttatttgaa acagtcagga atagttgatc
     1021 aattttttaa atcataggag gaaacaaaga tggaaaacaa aaacatgaat acaacaaatg
     1081 gatcagtttt ggcaaatgaa aaaggagaaa accgcatgat gaatggtgaa ttgacttatg
     1141 aagacaaagt catccaaaaa atcatcggtt atgcattgaa agatgtagat ggtctattga
     1201 cattagatgg cggatttttc tcaaatatgg ctgaaaaatt agtcaacaca gataacgtaa
     1261 cagcaggtat caatacagaa gtaggtaaga aacaagtagc tgtggatcta gatgtgatcg
     1321 tagaatacgg taaagacatc gaagatattt acaaccaaat caaagaattg atcagtactg
     1381 aagtaaacaa tatgacacat ttagatgtta tcgaagtgaa tgtgaatgtt gcagacatca
     1441 aaacacaaga agaataccaa aaagatcaag aaacagtaca agacaaagta acagaagcag
     1501 caaaatccac aggacaattt gcttcacgcc aaacagataa agcaaaatct gctgtaggca
     1561 aaggtgcaca aaaagtcaaa gaaaataacg aaccacgtgt acaataagaa gtaagaagtc
     1621 agttaggagg aatttaaaat gggatttatt tgggcattaa tcgtcggcgg tatcattgga
     1681 gcaattgcag gagcaatcac gaataaagga tcatcaatgg ggatcatcgc aaacatcatt
     1741 gctggtttag taggttcagc aattggacaa gctttactag gaacttgggg accaacaatt
     1801 gcaggaatgg ctatcattcc atcattgatc ggtgcaatca ttttagtatt agtcgtttca
     1861 ttctttgcac gtaaattata agcaaattga agaaaaactt atgttgaaat gaagaacaaa
     1921 tttgagaacc gtagcgacac tcgctgcgg
//
LOCUS       DAYYHX010000293         1920 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000293 DAYYHX010000000
VERSION     DAYYHX010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.296"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..472
                     /locus_tag="ACHA6Y_002830"
     CDS             <1..472
                     /locus_tag="ACHA6Y_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFM8988850.1"
                     /translation="DAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVYTSNELLSS
                     LEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQYLEFLLVPV
                     LIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFLSILI"
     gene            483..1898
                     /locus_tag="ACHA6Y_002831"
     CDS             483..1898
                     /locus_tag="ACHA6Y_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8988851.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
BASE COUNT          712 a          236 c          333 g          639 t
ORIGIN      
        1 tgatgctatc aagatagagt tttttactat attttttggg acatccttat atattgtatt
       61 tcgaatgttg ccggtcgtaa tgatctcatg gttgttggtt agtgtctata ctagcaatga
      121 attattatcc tctttagaaa aaatacattt accgaaaaaa ataatgttaa gtattacagt
      181 agtgatcaga ttctttccga cttatcgttc tgaaataaga atgattaaag aatctttaaa
      241 aatgagaaac attcgcttgt ctttttttca gcctctgcaa tatttggaat ttctgttagt
      301 tcccgttctt atacgtgcaa ctatgattgc tgaagaaatg accgctaatg caataacaaa
      361 ggggatagaa tctcctgtga aaagaagttc ttactataaa acaaacatga gcttattaga
      421 ctatatcatc ctgttttccg ttactatttt tttcttaagt attttgattt gaggtgaaag
      481 aattgtttga atttaacaat gtttcttttt catataagga tgttaaggaa caagcgcttg
      541 ttgaccttaa tttttcaata aaaaaaggta gtcttacatt gattactggt tcttctggga
      601 gtgggaaatc cacactatta aagttaatga atggacttat tccaagactt tatgacggaa
      661 aattggttgg ggaaatttta tttgaaaata actcgctaca atccttaaca caagaaagta
      721 tatcaaaaaa tattggctac gtaagtcaag atcctagagg acaattcttt acaacaaata
      781 caacgagtga gttagtattt tcgatggaaa actttggatt tacaaaagag catatggata
      841 aaagaatcaa tgaattagct gaattattag atctttcaaa tatattagat aaaaacattt
      901 tttcgctatc aagtggtgaa cggcagaaaa tttctattgg atgtagttta tcgctaaact
      961 ctaaattaat tatattggat gaaccgtctt caaatttgga cttcaaagcg actaagaaac
     1021 ttgctgactt actttgtgat ttaaaaaata aaggatttac tattgttata gctgaacatc
     1081 gtttttacta tttgaaagaa ttaattgatc aagttctatt tgttaaaaat agaactgtaa
     1141 aaactacaac tattcagttg ttaaaaactg aaaacaataa cgcattaagg aaatttgaca
     1201 tttttaatag ttcgattgaa gttccctata tcgaaaaggt aagtgcagat gtattaaaag
     1261 ttgaagaaat tacttatgaa aatattctaa aagacataaa ttttgatgtc aaagttggag
     1321 atgttattgg tttggtaggg aaaaacggtg tggggaaaac aaccttacta agaatactca
     1381 tgaagattat tcgccctacc aaaggaaaga taatcgaaaa taaaaaaaat ttgtcatcgc
     1441 cgtttttagt aatgcaagaa atggattacc aatttttcac agaatcagtg aggagcgagt
     1501 tagccctggg gaatgaaata gttagtaaag atcagcaaga aaaaatttta aaaaaaatgg
     1561 aactttataa aatgaaagat caattaccgt ttgatttatc cggtggagaa aaacaaagat
     1621 tattggtttc tatagcttca ttgtcaaaaa ctaatttatt tctatttgat gaacctacaa
     1681 gtgggttgga ttatattaat atggaaagga ttgctgacct tattaaagac ttaaaagaaa
     1741 agggtgcagt agttatagcc actcatgatc cggaatttct ttataaaaca tgcaatagaa
     1801 ttatttattt aaaaaatggt ggaataaaaa aagatataaa acttagagta gaagattcaa
     1861 ctttgataga acagattttt aatgacataa cgaaatagaa ttgaggagaa tatatatgaa
//
LOCUS       DAYYHX010000294         1893 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000294 DAYYHX010000000
VERSION     DAYYHX010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.298"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(337..792)
                     /gene="tnpA"
                     /locus_tag="ACHA6Y_002832"
     CDS             complement(337..792)
                     /gene="tnpA"
                     /locus_tag="ACHA6Y_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM8988852.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1035..1893
                     /locus_tag="ACHA6Y_002833"
                     /pseudo
     CDS             1035..1893
                     /locus_tag="ACHA6Y_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
BASE COUNT          601 a          368 c          355 g          569 t
ORIGIN      
        1 acttacgtaa ctcaaaaata cttccaaagg tgttcgataa tccagtgatt ttctaggaag
       61 attatttctg cgccagcatt ttctaatgtt tgaatttgtg ttttgtaatc ttggttgctt
      121 gtgctaattc ttgaatagcc gtaaattgcc aaaatttcat caccctttct ggacataagt
      181 ttctgacacc agttaaaatc aagtctatca gtaacccata cacccgtgag gacgggtgta
      241 tttttaatcc ccctagaagg ggtcgacact cgctgcccct taaaagggga tttttaaaag
      301 accgccaact gctcatcttc actcacttac ttctctttat tttttaaatg gttctacgta
      361 ctctctttta cttataatat atcgaatgcg atcatcagct tcttgttcac ggatgtattt
      421 ttcatttgtc ttttgattca atccgactgt actcacatag tatctcttcg accaaaagga
      481 tttgtttccg tatttatatt tgagatttgc gtgctgctcg tggattagta ccgcactacg
      541 acctttcaga taccccataa aactggatat acatattttt ggtggtactc gaacaagcat
      601 gtgaatatga tctagcattg catgtgcttc tatgatttcc acatctttca tttcgcacaa
      661 cttcctaagt atttttccaa gatcttttcg taattgccca tagattactt ttcgttgata
      721 ttttggtgta aaaataatat gatacttaca attccaacga gtatatgcta aacttttatc
      781 gtcatttgac atgaaaaaac tcctttcgat ctgatgagcg gttggtagcc actcactagt
      841 atatcgaaag gagtttttat cgctaaagat acttcttcca cgcgtagaaa cgcgcggttt
      901 tcttgtttcg cacgctttac gcacaaaact gactgaagtc attaagaaaa taaaagggtc
      961 taaaactttt aagttttgta ccgaccccca aaagttagac caaaaaatct aacttttgga
     1021 ggtcggtatt ttttatggct aagtatactt ttgaacttaa attaaagatc gttcatgatt
     1081 acttggatgg taaaggcgga agtgattatc tggcaaagaa atattccatt aaggctccat
     1141 cacaagtgaa gcgctggatc aatgcctatc aagaatttgg tgaagagggt ctagtaagaa
     1201 agcgacaaaa gaaaaaatat tctgttcaat ttaagctgga tgcgatagag ttatatctaa
     1261 caagtgagct gtcctatcgt gaagttgcca acacacttaa catgaacaac cctaatttga
     1321 tcgctagttg gatgcgtcaa ttccgtgagg gtgggatcga tggactctca aaaacgaaag
     1381 gatgtccacc tatcttgtct aagaaaaatg aacccaaaaa taagaaaaaa tctacaacta
     1441 aagcaacgtc taaagaacgt gaacgcatcg aggagttgga gaaacgagtc cgttcccttc
     1501 aaattgaaaa tgccttttta aaagaattga ggaagctgcg aaaacaggaa gcccaacaac
     1561 gacgaatgaa gcaatcgcac gaatcatcgc aagcctccga ggaccgttta aattagttga
     1621 gcttctagaa actttaaagt tccctaaggc aatctttatg tactggcaaa aacgattgga
     1681 ccgtaaaaat ccagatcaag agattgaagc ggaaatgctt aaagttcggg agaagcacaa
     1741 ggattatggc tgtttacgga tgaccaatga actaagaaac cgcggtttct ctatcaataa
     1801 aaagaaagtt cagcgcctga tcaagaagct aggaataaaa gtaacgacat acacacgtaa
     1861 atcacggcgc tacaattctt atcgaggaca agt
//
LOCUS       DAYYHX010000295         1888 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000295 DAYYHX010000000
VERSION     DAYYHX010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.299"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1382
                     /locus_tag="ACHA6Y_002834"
     CDS             <1..1382
                     /locus_tag="ACHA6Y_002834"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8988853.1"
                     /translation="GEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNLETFQ
                     HSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRWFAFP
                     RLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSKKNNK
                     HKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNTTFEN
                     LTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFTAILV
                     KVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVGKFIR
                     AVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGYAQVY
                     GKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVISLAEI
                     AFPNEKIFR"
     gene            1398..>1888
                     /locus_tag="ACHA6Y_002835"
     CDS             1398..>1888
                     /locus_tag="ACHA6Y_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8988854.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLM"
BASE COUNT          638 a          313 c          348 g          589 t
ORIGIN      
        1 atggagaatg gagtgctgca cgacggatat tcattatatt aatggatgtt attgtataca
       61 atgcagcaat atatagtagt ttcttactga agtttcgtgg agagatccca ttacgaaatt
      121 tagaaacttt ccaacactca gcaattatta tttcagtgat ttttattgcg ttgaacattt
      181 tgttgggtgc atatgttttt tataatcgaa tgatcagtga catcgtgttt gtcacagtca
      241 tcggtcaggt attgatgaca ctagggatca tggtagtgac ttttgcgggc cgctggtttg
      301 cttttccacg tcttgttatc ttactttcat ttgttctcgg tacgcttttc ttaagtatct
      361 atcgtattct cgtatataaa ctttatttac gcgtaagtcg agataaaaag gtcgtcatta
      421 ttgggttaga aaaggatgtc gcaccagcaa tccaaaattt tggttcaaag aaaaacaaca
      481 aacataaagt cgaagcagtc attcttgata actattatgc aaacatcaaa aatatgatta
      541 aagacatcga tattgtctat cttgcttctc atatcgaaga aactgaaaaa atcaaaatct
      601 atcaattagt tactaaaaat gaaaaaaaat tgtttttgaa tacaacattt gaaaacttga
      661 cattgatcaa tccaaatatt atgaatttcg aagatgaaag tattattgaa gcatctggat
      721 ttagaatcca acctgaatat gaactattca aacgtttgtt tgatatcatc gtttcacttg
      781 ttttattagt tattgcttca ccatttatgt tattcacagc aattttagtc aaagtgacat
      841 caccgggacc aatcatctat aaacaagtgc ggatcacaaa aaatcaaaag gaattttcaa
      901 tctataaatt ccgctcgatg accgccacag ccgaagcaaa atctggacca gttttagcta
      961 agagcaatga tgcacgagta acgcctgtag gtaaatttat tcgagctgtc cgctttgatg
     1021 aattaccaca aatcttcaat gtgctgaaag gcgatatgtc gatcgtagga ccacgtcctg
     1081 aacgtccttt cttcgtcgat cagttcaatg cagaaaaccc ttattactac ttacggcata
     1141 atgttcgtgc tggtatcact gggtatgcac aagtctatgg aaaatatgtc tctgattaca
     1201 acagtaaatt acgttttgat ttactttata tcaaaaaata ttcattgatg cttgatttaa
     1261 agatcttgct gcaaaccatc aagatcctat ttgataaaat gtcctctcaa ggacttgaag
     1321 aagacgaaaa tgtgatctca ttggcagaaa ttgcttttcc gaatgaaaaa atctttcgct
     1381 agttgggagg cgtaaaaatg aacaatccga aattcagcat aatcgtacca gtatacaatg
     1441 cagaagcaac tattggtcga acgattgata ttctaaaaga attggattat gaagagtacg
     1501 aagtcgtttt agtaaatgat ggatcaacag atcaaacaca ggaaatcatt caagaactga
     1561 ttgaaaatga tgaacgtttt gaattgatta ctacgaccaa tcaagggccc ggattagcaa
     1621 gaaacgaagg aataagacag tcaaaaggag aatatctctt attcttcgat gcagatgaca
     1681 cgccagtaaa aacgatcttg tcagattatg atcatttgct agaaaagagt ccaaaagcgg
     1741 atcttgttgt tagttcgttt gtttttcgta caatggacga gggaagaatc gtttccgaaa
     1801 aagaaaactt agttacagaa tgtcactatc taactcacga agagtttata aaagacatgt
     1861 atcagttaat gaatgaacag ttgatgta
//
LOCUS       DAYYHX010000296         1886 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000296 DAYYHX010000000
VERSION     DAYYHX010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.300"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..1836)
                     /locus_tag="ACHA6Y_002836"
     CDS             complement(13..1836)
                     /locus_tag="ACHA6Y_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8988855.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT          628 a          401 c          356 g          501 t
ORIGIN      
        1 ttaaaacttg gtttatttct tcaaatggta gctataggtg ctgacaacga tattttcacg
       61 ccaagacaat cgcaatctta accgcaaact tgtttggtta tggagaatat tctgccagcg
      121 tctgtttggt acaaattgag gaatcaacac agtagttgta tagttttgtt tcgctgcatt
      181 tttacttacg atatctacat aacggataat tggattttca atcgagcgat aagaagagtg
      241 gacgattgaa aaacggacat ctgggaaatg ctttttaaat tctgcttcga tttctttttc
      301 tttttcgaca tcttcgtcta gcgaaacatg catcgcaacg acataatcac cgatcgaacg
      361 tgcgtaattc aacgcaccga tatttacccg tgtcacattc ccgactaata caagaacggt
      421 attcccgtca taatcatgta gttttgcatc attttctaaa cgaagctgtt ctgctacttt
      481 tttataatga tcatggatct tataaaaaat gaacataacg attggcataa tgatgaagaa
      541 aggccaaatg tcccctaacc gatagacaaa caaaatcgca atgattgcat atgagatgaa
      601 cgcacccgta atattggcaa tcgatttgct taaccatctt tttccttctt ttttccattt
      661 gacgaccatc cctgtttgag ataaagcaaa tggaatgaac acgccgatag agtataacgg
      721 aatcaagcgt tctgttgagc catggaaaat aaacagcaat acgatcgaac ctagcgctag
      781 tgtgataatc ccattggaat agcctagtcg atccccacga tcttgataca tgtgaggcat
      841 gaatttatct tttgccagat tgtatgcaag aactggaaaa gcagaaaaac cggtgttagc
      901 agcaactgct aaaatcagcg ctgtagcaaa ttgaaggacg taatacaaaa taccatgtcc
      961 aaagacagct ttaccaattt gagacaaaac agtgacttct ttttcaggaa cgatcccata
     1021 ccaatagttg ataaatgtga tccccacgaa gaagaaacct aagatcaatg ccatcatcgt
     1081 caatgtagca gcggcatttt ttgctcttgg cttcttaaag aatggtacag cattactaat
     1141 cgcctccacc ccagttaaag aggaagaacc agaagaaaat gctcgtaaaa tcaaagcgat
     1201 agaaacaccc ggcacagccg ctcctggaag tgcagtagct tgcaatggca cagcacctgt
     1261 cacgatatta tataaaccaa caacgatcag taacgagatc acaaggataa atgtgtacac
     1321 cggaaataat aggaacgatg cagattccct cagcccacga agattcagca tcatcagtag
     1381 caaaacgatt gtcacagaaa tagcgacttg atgaccgtag agtgctggca ccgctgatgt
     1441 gatcgcttca gcacctgctg agacagatac tgctactgtc aacatgtagt cgatcaataa
     1501 agagccaccg gaaatcaagc cagcattctt tccaagattc tcactgctga cgacatatgc
     1561 tcccccgcca tgaggataag catggatgat ttgacgatat gatagcgtta atgaaatcaa
     1621 taaaataata acaaaagcgg caatgggtag cgaataccag atggctgcag cagacaaagc
     1681 gactaaaacg acaacgattt gttccgtacc atatgcaata gatgataacg catcagacga
     1741 caataatgcc aaagcagcaa atcgggttaa tttgtgctca tcattttcag cagattttaa
     1801 aggcttacct actaacaacc tttttaagta atccaatttc taaactcctt tacacacaat
     1861 tttacttcaa gttttatagt cgaaaa
//
LOCUS       DAYYHX010000297         1859 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000297 DAYYHX010000000
VERSION     DAYYHX010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.301"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="ACHA6Y_002837"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="ACHA6Y_002837"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8988856.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DAYYHX010000298         1849 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000298 DAYYHX010000000
VERSION     DAYYHX010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.302"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..729)
                     /locus_tag="ACHA6Y_002838"
     CDS             complement(<1..729)
                     /locus_tag="ACHA6Y_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8988857.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKV"
     gene            complement(689..>1849)
                     /locus_tag="ACHA6Y_002839"
     CDS             complement(689..>1849)
                     /locus_tag="ACHA6Y_002839"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8988858.1"
                     /translation="EYIRGIQVIKIFGAKVTSFKALNKAINDYAKFAYKYSLSGKTPF
                     VLFQWLFFGIVCILIVPITFFITSLTTPKILAVDLLMLFFLSGIIFVSFMRIMYVSMH
                     LFEARYAVETLDSLYLEMKQDQLKYGNETQIKNCDIAFENVSFSYKEECVLENFNLVL
                     EQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQIKIGGKPLNHYSKDTIINTIAFVFQ
                     DTKLFNQSIYDNVSIANPQAPASKVFDALRLAGCESILEKFPEREHTLIGSKGVYLSG
                     GEKQRIAIARAILKDAKIVIMDEASASIDPDNEYELQKAFQHLMKEKTVIMIAHRLSS
                     IKSVDEILVLSEGKIVERGNHNELMAKHQVYKQLVTNYENANDWRLDNEKFL"
BASE COUNT          609 a          337 c          256 g          647 t
ORIGIN      
        1 tacctttaaa tgaatccttt cactctcttc catttttttg aaaagtctgt cttgaacttt
       61 tttagataga ttaaagctat taatctcttg ctgcaattct atagtttctt gaaattcttc
      121 cgagttttct ctcaaagtat cgtaatattt agtattggct ttcgtttggg atttctttga
      181 taacaagatt aagacaaaac taaataacgt tggcaaaata acagctaatc ccattttgac
      241 attaccaacc aaaagcatca ctgaaataaa agggaaaaac agagccatac cgccggcttt
      301 aggtattgca tgactcatcg catgctcaat accttcaacg tcagacatga tagtttgaga
      361 taaatctgat aaattatgtt ttgaaaaata ggataatggt aaatttgaca aatcgtctgc
      421 aatttgcatt cttaaatgcg cactttcttt ataagtacta ttatataagt tttcatactc
      481 cctatctaac aaccaataca tgaaaagcaa agtaacgaac gatactgtaa aataaagcca
      541 aggcggcttt acattttcta aaactaattc gtctatcagc atcattataa tgaatgctgg
      601 taccatatta atgcagtata caaaaaagct agaaatagat gctttaacca aatcttctgc
      661 gccctgatta gttaaagcga atttgttttt ataaaaactt ttcattgtca agcctccaat
      721 catttgcatt ctcataatta gtgaccaatt gtttatacac ttgatgttta gccattaatt
      781 cattatgatt tccacgttct actattttcc cttctgacaa gacaagaatt tcatctacag
      841 atttaatgct ggataatcga tgagctatca taataactgt tttttctttc atcagatgtt
      901 gaaaagcctt ttgtagttca tactcattat ctggatcaat agatgcactg gcttcatcca
      961 taataacaat ctttgcatct tttaaaattg ctctggcaat agctatacgt tgtttttctc
     1021 ctcctgacag ataaactcct ttactaccaa ttaaggtatg ctctctttcg ggaaattttt
     1081 ctaaaatcga ttcgcaaccg gctaatctta gcgcgtcaaa tactttactt gctggcgctt
     1141 gaggatttgc tatagaaacg ttatcgtaaa tactctgatt gaatagtttc gtatcttgaa
     1201 agacaaaagc aattgtgtta ataattgtgt cttttgaata gtgatttaaa ggcttaccac
     1261 caattttaat ctgtccctta tccacattgt agaaacctga aattagttta gctatcgtac
     1321 tctttccgct tcctgatttt cctactaatg cataaattct tttttgttct aaaactaagt
     1381 tgaaattttc taaaacacac tcttctttgt atgaaaagct gacattttca aatgcaatgt
     1441 cacaattttt aatctgtgtc tcgttcccat attttagttg gtcttgcttc atttctaaat
     1501 aaagggagtc tagtgtttcg actgcatatc ttgcttcaaa caagtgcata cttacataca
     1561 ttattctcat gaaagaaaca aaaataattc cacttaaaaa aaacaacatc aacaagtcaa
     1621 cagctaatat cttgggagtg gtaagagaag ttataaaaaa ggttatagga actatcaaaa
     1681 tgcaaactat gccaaaaaat agccattgaa aaagaacaaa aggtgttttc ccactcaaag
     1741 aatacttata tgcaaactta gcataatcgt taattgcttt attcaaggct ttaaatgatg
     1801 ttactttagc accaaatatc tttataactt gaattcctcg gatatattc
//
LOCUS       DAYYHX010000299         1835 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000299 DAYYHX010000000
VERSION     DAYYHX010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.303"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..735)
                     /locus_tag="ACHA6Y_002840"
     CDS             complement(34..735)
                     /locus_tag="ACHA6Y_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8988859.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(751..1191)
                     /locus_tag="ACHA6Y_002841"
     CDS             complement(751..1191)
                     /locus_tag="ACHA6Y_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8988860.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1191..1766)
                     /locus_tag="ACHA6Y_002842"
     CDS             complement(1191..1766)
                     /locus_tag="ACHA6Y_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM8988861.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          517 a          400 c          283 g          635 t
ORIGIN      
        1 aatttttctt tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg
       61 ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg
      121 gatctgacgg atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc
      181 atccacttgt attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt
      241 caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc
      301 acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc
      361 aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc
      421 gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag
      481 gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat
      541 tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt
      601 tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc
      661 aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat
      721 caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac
      781 aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag
      841 actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt
      901 tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc
      961 aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta
     1021 tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg
     1081 agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg
     1141 atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc
     1201 tccttcaata ataactgatt tttaatagat actgctcgta attcattgac cagttccaat
     1261 ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca
     1321 aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt
     1381 ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat
     1441 gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt
     1501 ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt
     1561 tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag tactgtttta
     1621 ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt
     1681 gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg
     1741 gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc
     1801 attctacacg tgttttttta aaataaaaga ctaga
//
LOCUS       DAYYHX010000300         1819 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000300 DAYYHX010000000
VERSION     DAYYHX010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.304"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            537..869
                     /gene="traE"
                     /locus_tag="ACHA6Y_002843"
     CDS             537..869
                     /gene="traE"
                     /locus_tag="ACHA6Y_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM8988862.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1488..1787
                     /locus_tag="ACHA6Y_002844"
     CDS             1488..1787
                     /locus_tag="ACHA6Y_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8988863.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNEWFNRIRQKSPILYRWVMNAVRYEGYRW"
BASE COUNT          611 a          268 c          389 g          551 t
ORIGIN      
        1 caatacgaat attattcgct tacatttttt tgtataaatt tagttgtttt aaatttaatt
       61 gtctgctacg cttgtgatga attaattttc gttctgcact tttggacgat aaatttggac
      121 aattgaaaat acaaaaaaaa gaaaggccct gtgagactcg gaaggctggt aaccttcgtc
      181 gacgtccaca ggtggcatta agacaacctt taatccctta atgcgtttac ctttcagtgt
      241 tacggtatta cccgcaacgg atttagtata acagatattc aaaagtgata ccactttttt
      301 tggctgtgtt aaggttaaaa atacgtgata ttacgatgaa tctatgccta agcataatgt
      361 tataaaaatg tccgtgtggc tctttgccgt atgacgaacg ctgaaattag agggaacgca
      421 tgtttggatg cgttccctct tttttgtttg tcatgaacga aaatcaattc tctatttttg
      481 aaaaataaca agaaaaactt attaaaaata tttaaagtaa gtgggtggat tattttatgt
      541 catatacagg aattttgagt ctcgaagata tttgtcacta cggtaaacgg tgtaccgcta
      601 cagagaaaat cacgaagaaa ctatcaactg gccaaagtaa aaccgttgtg cagtgtaaga
      661 aatatataat ccagaaagac aaagtctcag aggaaatgat ttattacgct ggtaaacaaa
      721 agcaaataat tttaaaagat cctatccctt taaaagaact atatccaaca atcaaacatg
      781 tttatgacca aaacggtgta ctgattggtc gaagaaaaaa tggagtcttg cgttgtaccg
      841 caaaagggat ggggcgttta atcagctgaa ttgatcattc tcataacttg tattttatcg
      901 tttttcgctt gcttgaaaat attgtcgaga cttctaaaag aacttagaat tactagaaaa
      961 ataaggagta gacttgatct atcaacaatt cctttcgggg ggtgacgggg taggttttta
     1021 cgattggtaa cgtaaaaaat tggtcaatcg agtaaatgcc cttgtttgga tagtggtgaa
     1081 atgaatttgt gtaaataata tgcacagagt aaacaagagg gaatttagaa gttagacaag
     1141 ttagaatagc gcctagatta atcatgagca gctactaagg gatctaggct ttgacgcaaa
     1201 ctcatggaat aaggagtagg tcttctcgta atggggagga cgcacaagaa attgtgtcgt
     1261 gtgctgcagt acatgtagat acttgcgagg acgttggtca ttggatcaat cgagcattta
     1321 ccgatttcta ctagggtttc tcgtttcgga cgccataaac gagtcgatat tttttatgta
     1381 tttttaagta aacgaaaaac gataaaatat gaacgagtcc tgaataaagt gaaaaaattg
     1441 atataaaaca tagagaaaat gaaatttacg taatggagga atgacaaatg aaaaattatg
     1501 atccgaatat acgattgggc acacatacta ttaaggtttc tttccaacgt tgggattaca
     1561 aaggatttgt gacgtttaga agaggcggaa attgtaaagg attagatgtt ttagcattag
     1621 acgaggatga tttatactat caaaaattga ctgataatcc aattggattc ggcttgctac
     1681 ctgaagatga tgaaggtaat gaatggttta acagaattag gcagaagtcg cccatcctct
     1741 ataggtgggt gatgaatgcc gttcggtatg agggttaccg ttggtaactc gaagaccgac
     1801 aactaaggcg tattgcctt
//
LOCUS       DAYYHX010000301         1702 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000301 DAYYHX010000000
VERSION     DAYYHX010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.305"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..646)
                     /locus_tag="ACHA6Y_002845"
     CDS             complement(104..646)
                     /locus_tag="ACHA6Y_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HFM8988864.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
     gene            complement(959..1510)
                     /locus_tag="ACHA6Y_002846"
     CDS             complement(959..1510)
                     /locus_tag="ACHA6Y_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8988865.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
BASE COUNT          486 a          337 c          313 g          566 t
ORIGIN      
        1 aaataaccca catgaatgat ttgaaatcgt cttaaaatgc tttattttat aacccaaaat
       61 agaataatta cttcaaaatg acgtcgcata tcactattaa tgattaatcg atgacttcca
      121 taatcatttt aagttccccg gatgttaaat cctcgctaaa aatgccagta actttttgtc
      181 caggtccaat ttcctttgat gataggttta acaaagagat agggatatag attaatccgt
      241 tttgtttcaa agacagagcc aatatttttt tccttgttcg aatagctgca tcatagcgaa
      301 gagaagagta aacacctctg ccaacaatag aaatatgctt ccccaataat aaatcaaggc
      361 tacctattac ttgtaatttc tgaaaggtcg tcccatccgt tttgatttga atatcctgta
      421 aatttatttt cctgtctaag gcatcttgaa aaaacaagtg tgtgcctctg cgataatcaa
      481 tgccacagag gtgcataaaa ttagttgtcg aaaaaataat cgctaccgtt gagacttctc
      541 cgacaaagga tactttttta tctacaaaat gttcttgtaa gagatgagca gccttataaa
      601 tcgcgtttaa ctgtctggta atttgggttg tctcttttat agacatgcgt ttgtattctt
      661 tttttgccgc gtgcgaagaa tgctcctttt tcccaataaa aaaagctgct caaaagagca
      721 gccttttgta caggtttagt tttggtgctt gccacccgcc ggacccttac gttccggatt
      781 tgtatcgagt ttagttttgg tgctcgccac ccgccggacc cttatgttcc ggatttatat
      841 caagttgact atgagcctgc tctcatttgt atatataata taccaaaaaa aggttgattt
      901 gtccaatact gtgggtgatt ataaccgtct tactgtgctt atctattata taatacaatc
      961 aaaaacctaa ccgaaaattt aataacgttt cactgatttc atcttcatta attccgatgt
     1021 agcgtttggt aatcttctcg ctgctgtgac caaagatttc cattaaggtg gccacgtctt
     1081 tggttttctt gtaatagtgg taaccaaagg tttttcgtag ggtgtgggtg ccgatatcgt
     1141 cccttcctaa cagcttcgcg accttttgaa acatctggta gaccgtattg acttctaaat
     1201 ggccgccctt agtactggga aacaagtaag cctccggatc taagggtgcg gtatagccgt
     1261 gaatcaagtc ctgtaagctg cttaagtata aaatgcgtgt tttccctgtc ttttgctcca
     1321 cgatgcgcgg gttttttgag gtaatcacgt cttttttctt gagtttaacg atgtcggaca
     1381 tgcgcagacc gctgttaatt ccaattagaa acaagaaaac gtcacgttcc gcatttttat
     1441 tccgtcgcag gcaaaataaa aaatcattaa tctcctgttg cgaccgtagt ggctgaacat
     1501 tatagcccat gacaatccct cctttttagt ctgttcgaat acatgattat agttattaca
     1561 acacgaaatc gtgtgtttag ggggagtaaa aaaaaagaac cctataagga taggattctt
     1621 gatacatgat tttatccaat ttcatgtatc ttatattcaa cccgcgccac aagagtatgg
     1681 ttgatatatc attttgactt tg
//
LOCUS       DAYYHX010000302         1701 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000302 DAYYHX010000000
VERSION     DAYYHX010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.306"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..313
                     /locus_tag="ACHA6Y_002847"
     CDS             20..313
                     /locus_tag="ACHA6Y_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFM8988866.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            306..602
                     /locus_tag="ACHA6Y_002848"
     CDS             306..602
                     /locus_tag="ACHA6Y_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988867.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            677..1675
                     /locus_tag="ACHA6Y_002849"
     CDS             677..1675
                     /locus_tag="ACHA6Y_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8988868.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
BASE COUNT          656 a          232 c          337 g          476 t
ORIGIN      
        1 aataacaggg ggtgtgtaga tggagattga tgaaacttgt ttgagtaact cttataaaag
       61 tatgaatgac ttttttgagg aaatggaact ggattcagaa ttgctctatc aattttatct
      121 tgcttttcga gggcaacaga tttcttttcc aatgaaaatg tacgatcgtg agttagttag
      181 aaaaagaatc gaaaacatga tagaacagga aaaaactgta gatatccgtc aattgacaga
      241 agtttatggt ttctctgcaa gatggattca aaaagtaatt aaacaaaaag aaaggagacg
      301 agtacatggc taagctgatt gtattaggtg gtttaattta cggtggatat tggttatatc
      361 aatatatgct tgcgcatccg aatatagata aaggaaaagt aattaagaca gttggggtaa
      421 tcatagttgg actcatagct ctaactaaga aaaataatac taatagccca aaaataatga
      481 gaacttatcg ctgtagagga tgtggaaggg aaatttctac tagttttaat ccatctgaga
      541 gtcctcaagg aagagaccgc tgccatacgg gctatcgaca ttattgggaa agaatcgatt
      601 aaaagattac catttagatc aattaattgt ctgatagaat aagtacgtga gggagaaatt
      661 gaaaggggga aatataatga aaaaatggct gttattacta ataggaatgg gaattttatt
      721 cactggttgt acatctgaga atacgaggac taaagcgact atagaaactg atacaacaat
      781 tgagactaaa aaaagtaaaa cgcaatcaac tgaacaaact acaacagaaa tgaagcaatc
      841 tgtggaaaca agtgcaagtt caatcactga atcttcttct gaagaggtta agaatacttt
      901 gtgggatacg aataaagctt ccaaattaga aacttttgtc acacaatggg gaaagacatt
      961 aggtcaagaa tataaaagct acaatcttca gaataatgta agtctgtatg gcactccttt
     1021 accacaagcc gtgattaatg gcgattggaa aatggcaatc aatgaagcac cagttaccgt
     1081 acaatggtca gaagatggaa ctggtcatgc agatttcaat ttagtagcag tttattctga
     1141 tgtagaaact ggagaatatt tagggcagca tgtttacttt ttcggttttc aaaatggaca
     1201 acctaaagtt tatttaacgc aacaaaatca aggtaatgaa aataattatc tgtactttaa
     1261 cgaaactcaa aatcaacagt taaaacaagg tttcagtgat attgtgaatg gacaaactcc
     1321 tcaaacacca gtagtagaag aatctactca acaagcaaca actacaacga cacaatctac
     1381 aagtagttca ggaatttcaa gttgggaagc tgctaaagaa tctgtgctaa ataataaagc
     1441 tttatggttg tatgattctc ctgataaaaa tgtatcaaat actaatttaa ttagcacaaa
     1501 tgaatatatg aaagatctaa tgcaagatga aaatggaagc tactattcaa ttaatattgt
     1561 aacggatgat gatggaaaca cgaccatggc tggtggaaaa caatttcgtg tttatacaga
     1621 tggaagaatt tctcaacgta ttggaatgac taacaatttt caaatgatta aataattgaa
     1681 aatattattt atcaaagaaa a
//
LOCUS       DAYYHX010000303         1593 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000303 DAYYHX010000000
VERSION     DAYYHX010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.307"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            287..694
                     /locus_tag="ACHA6Y_002850"
     CDS             287..694
                     /locus_tag="ACHA6Y_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988869.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="ACHA6Y_002851"
     CDS             708..1109
                     /locus_tag="ACHA6Y_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988870.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="ACHA6Y_002852"
     CDS             1111..1482
                     /locus_tag="ACHA6Y_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988871.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          596 a          201 c          277 g          519 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tcc
//
LOCUS       DAYYHX010000304         1584 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000304 DAYYHX010000000
VERSION     DAYYHX010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.308"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..>1584
                     /locus_tag="ACHA6Y_002853"
     CDS             275..>1584
                     /locus_tag="ACHA6Y_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8988872.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVI"
BASE COUNT          574 a          221 c          287 g          502 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttga
//
LOCUS       DAYYHX010000305         1563 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000305 DAYYHX010000000
VERSION     DAYYHX010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.309"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..699
                     /locus_tag="ACHA6Y_002854"
     CDS             133..699
                     /locus_tag="ACHA6Y_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8988873.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            844..931
                     /locus_tag="ACHA6Y_002855"
     tRNA            844..931
                     /locus_tag="ACHA6Y_002855"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:876..878,aa:Ser,seq:cga)
     gene            complement(983..>1563)
                     /locus_tag="ACHA6Y_002856"
     CDS             complement(983..>1563)
                     /locus_tag="ACHA6Y_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8988874.1"
                     /translation="FSLLAFTGIRKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLI
                     VNTPKSKSGKRKIYLDNDLISLLRKYYTKNKTIVTIQRENLIFHHDGLLYNPTVSRSW
                     LNVIYKHHPELTKRITTHGFRHTHASLLFESGASLKDVQERLGHADIQTTSNIYTHVT
                     ETQNKKVINNFVAFMKNSTQGESKGESKNILG"
BASE COUNT          523 a          240 c          297 g          503 t
ORIGIN      
        1 tgcaaaaaaa agttaaagaa gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat
       61 gtttgcaaaa aatgctaaaa atagggtaca ataaaacaac atatactgca gtaaagaagt
      121 gggggagttt taatgagaag aaaagtctat accaaggatc aaattctaaa agctgcttat
      181 gatgttgttg caaaggaagg gtttaaagga tttactgcga gaaatattgc taaaaagatg
      241 gggatttcga cacagccgat ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg
      301 ttgctggatt ctatctttga acaactaaaa gaagatgttt tccctattga acgtactggt
      361 gataaaatca ttgatctcgg tttgaactat atctactttg caaaaaagca tcatagtctt
      421 tacatggcat tgtacattga tgaacatggg gacggaaaga aaatgcatga tttttcatac
      481 aattatttcc gtgaaatagt taaagaagat gaaagataca atcaattatc ggatgaatat
      541 gtcgatgctc tgcatactgg tacatgggta gcagtgactg gcttagcgtc attgatgtca
      601 tcaaacatca tgcatccaac agaagaacaa atcattgcac tgatcaaacg gacaattgac
      661 tcgattttag cattagataa tcctgaaata atcagatagt gttctatcag tatgaatttg
      721 atcggtaagt ccgttggctg caaactgtca atggactttt ttgctaaaaa aagcgatttc
      781 tgttatttat ctattagaaa taggcttgac atttcgtgaa aaaagtttaa aaatagtact
      841 tacggaaggt tggcagagtg gtaatgcacc ggactcgaaa tccggcgaac cggctaatac
      901 cggcgcgcag gttcaaatcc tgtaccttcc ttatatatca acgattagac acgatttcga
      961 tcgtgtcttt tttgttgtat atttagccta aaatgttttt tgactcccct tttgactccc
     1021 cctgagtact gttcttcata aatgctacaa aattattgat gacttttttg ttttgagttt
     1081 cagttacatg agtgtaaata tttgaagtag tttgaatgtc tgcatgccct aaacgttcct
     1141 gaacatcttt taaagaagct cctgattcaa aaaggagaga ggcatgagta tgtcgaaaac
     1201 catgagtagt gattctttta gtcaattctg gatgatgctt atatatgaca ttaagccacg
     1261 aacgtgaaac tgtagggtta tagagaagac catcatggtg aaaaattaag ttttcccttt
     1321 ggattgtaac gatcgttttg ttttttgtat aatactttct caataaactg atcaaatcat
     1381 tgtccagata aatttttctt tttccagatt ttgatttagg agtatttacg atcaatcttc
     1441 cttgataacc tcttgttaca gttttattga tattgagtgt tttgctctta aagtcaatat
     1501 ccgaccaggt cagagcaaaa gcttctcctt ttcttatacc tgtaaaagcc aatagggaaa
     1561 aga
//
LOCUS       DAYYHX010000306         1532 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000306 DAYYHX010000000
VERSION     DAYYHX010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.310"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1518)
                     /locus_tag="ACHA6Y_002857"
     CDS             complement(52..1518)
                     /locus_tag="ACHA6Y_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM8988875.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          501 a          325 c          289 g          417 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aa
//
LOCUS       DAYYHX010000307         1526 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000307 DAYYHX010000000
VERSION     DAYYHX010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.311"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..734)
                     /locus_tag="ACHA6Y_002858"
     CDS             complement(<1..734)
                     /locus_tag="ACHA6Y_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8988876.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYTINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSAMKYGIPLTLETPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTL
                     TDGTNYAHIDQFGEIENANLHVAGWH"
     gene            complement(731..1150)
                     /locus_tag="ACHA6Y_002859"
     CDS             complement(731..1150)
                     /locus_tag="ACHA6Y_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFM8988877.1"
                     /translation="MEKYLNTFSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVEVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(1220..1360)
                     /locus_tag="ACHA6Y_002860"
     CDS             complement(1220..1360)
                     /locus_tag="ACHA6Y_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8988878.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
BASE COUNT          417 a          327 c          236 g          546 t
ORIGIN      
        1 atgtgccatc cagcgacgtg taagttcgca ttttcgattt ctccaaactg atcaatgtgg
       61 gcgtaatttg ttccatctgt cagtgtagga tttgctgcac ctgctctagt tggatcaatg
      121 actggtttat catctgaagt tgtcggattt tcatcggtaa atccatgagc taaatcatat
      181 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
      241 ccccagatat tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggcgtt
      301 tctaatgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
      361 ttgtagttct tttcaaacgt tactttatcg tgcgtatgtt gcagttcgat ttgcacagga
      421 ctatttgcat tagcatatga accagcgcca tattgtacat aaccaggttg tccaacttgg
      481 taaacgatcc caccatcgcc aacaatatac gctgtatagg cattgtacca agaacgtttc
      541 atgtactgcg cttcatttcg tcctgtcgca gtttcgtttg ctgtttcatg caataggatg
      601 tattggttat ttgctacttg agaactacct tcgtttgcgc ccaaattgaa ttcattgttg
      661 atcgtgtaag caaaggcgtt tattggcaat aaaaaaagag ctataatagc tcctacaaag
      721 attttctttt tcatttggtt cctcctcggt ctttattttc agtatcattt cgtatttgtt
      781 ggaatacctc ttttaactta tctggcaaag gaaggaatac cgaaatgttt tctaaaaaac
      841 taatcccttc atttgcaaca tagaacataa taactatttc tcttagaggt atagaattcc
      901 caacgatttt ctgtacttca accgcaacgg caatcacaac gaagattaag actttcttca
      961 acaatccttc gaaacctatc tcactactta tttctttcaa tttccatgcc ttagcaagtc
     1021 cagttaagaa gtccacaccc acaagaaaaa ttaaaacatg tagtatattg tccatccctc
     1081 ccaataaacc aacaattgtc ccgcctatca cccctgtcat tactgagaat gtatttaaat
     1141 atttttccaa tatctatttc tcctttatgc aaaaaagcgc actcgtttga gtacgctttt
     1201 tatttaactt tgtgcttaat taaatttcat ttccagtaat ctgcttatat tggtcttttg
     1261 ttaaacagtt catgtttaca tagtcaagta aatcttggtc cgtataacat ccccaatcat
     1321 aaaattgttt tatattttca aagcccggaa aactattcat tacttttccc tccattctta
     1381 atacttgcga cttccatcgt caattgagct aacatttgtt gcattttttc ttgttcctgt
     1441 accgatatcg ccaattgttt tgtcagtaga gcatttaatt gcttcaactg atcgacatct
     1501 tcaatatcgt caaccgctgt gccact
//
LOCUS       DAYYHX010000308         1514 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000308 DAYYHX010000000
VERSION     DAYYHX010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.312"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..1439)
                     /locus_tag="ACHA6Y_002861"
     CDS             complement(147..1439)
                     /locus_tag="ACHA6Y_002861"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM8988879.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          422 a          339 c          264 g          489 t
ORIGIN      
        1 tctgaataaa cagtgttcaa gagacagcct atcggcaata aggctaaacg tctctttcac
       61 tacctcttca agttattatt atcgtataga taaacacttc gactcgctct ggtaagaacg
      121 tcctgattca gtcgtcgttt ttatccttag atttgcgccc agacgctttc taagatgttt
      181 gtttgtgttc tatcaggtcc gacagaaaat gttgagatac gtacaccaac aagctctgag
      241 atacgacgta catagttacg tgcgttttct ggcagttctg caagtgtttt gcatcctgta
      301 atgtcttctg accagcctgg caattcttca tagattggtt tgcagcggtt caactctttc
      361 aagcttgctg gatagtggta gattagttcg ccatctaatt cataagccgt acaaattttt
      421 actgtttcca atccacttaa tacatcgatc gagtttaaag ataggttggt aatacctgaa
      481 actcgtttag aatggcgcat aactacagaa tcaaaccagc ctacacgacg cggacgacca
      541 gttgtcgtac cgtattctcg tcctacttcg cggatttgtt ctcctgtttc atcaaataat
      601 tcagttggaa atggtccatc accgacacgt gacgtatagg ctttgcagac gccgacaact
      661 ttattgattt ttgaaggccc tacaccgctt ccgattgtta ccccaccggc tactgggtta
      721 gaagaagtaa caaaaggata agttccctgg tcgatatcca gcattacacc ttgtgcgcct
      781 tcaaacaaca cgcgtttgcc tgcatccaaa gcatcattca agataacgga agtatctgtg
      841 acgtattgtt tgatttgttg tccgtattca taatattctt caaaaatatc gtcaaaatca
      901 agtggttctg aatcaaacat ttttacaaat tgacggtttt tgtcatctag attcaaacga
      961 agacgttctt caaaaatttc tttatctaat aagtctgcaa tacggatacc tacacgagct
     1021 gctttatcca tataggcagg tccgattcct ttaatcgttg taccaatctt attttcgccc
     1081 ttagcatctt cttgtaattg gtccaatttg atatgataag gcaagattac gtgcgcgcgg
     1141 tcagaaatac gcaaattact tgtttctaca cctttttcat gtaaataagc taattcttta
     1201 accaatgatt taggattgac tacaacgccg tttccgatca cactgatctt gtctttataa
     1261 aaaatcccag aaggaatcaa atgcagttta taagtcacac cgttaaactt aattgtgtgc
     1321 cctgcattgt cgccaccttg gtaccgtgca atcacttcag cattttcact gagaaagtct
     1381 gtgatttttc ctttcccttc atcgccccat tgcgtaccta ctactactac agatgacatg
     1441 tgaacacctc gttctatatt atttaatcct gaaccattgt atcaataata gatacatatt
     1501 tcaaataaaa tcga
//
LOCUS       DAYYHX010000309         1508 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000309 DAYYHX010000000
VERSION     DAYYHX010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.313"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..432
                     /locus_tag="ACHA6Y_002862"
     CDS             <1..432
                     /locus_tag="ACHA6Y_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8988880.1"
                     /translation="QVIMNPKVEYIYEFEGGKGITNQYRPNKFETFKEFYSLANTVTD
                     DINKDGMAALLLKGTTSVIFSIYSTSARTSKEKKDEAKQILSDPVIREAKQIAKTDSS
                     AKKITKLLYNMPAPLFLSAVKVGSFVEVKMPGLMAALKRVY"
     gene            451..>1508
                     /locus_tag="ACHA6Y_002863"
     CDS             451..>1508
                     /locus_tag="ACHA6Y_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8988881.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQ"
BASE COUNT          568 a          193 c          292 g          455 t
ORIGIN      
        1 caagtgatca tgaacccaaa agtagaatat atttatgaat ttgaaggtgg gaaggggatc
       61 actaatcaat accgacctaa caagttcgaa acatttaaag aattctattc attagcaaat
      121 actgtaacag acgatattaa taaagacggt atggcggcgc ttcttttaaa agggacaact
      181 tcagttattt tttctattta tagtacctct gcaagaactt caaaagagaa gaaagacgaa
      241 gcaaagcaga tattgagtga tccagtgatc cgagaagcca agcaaattgc taagacggat
      301 tcttcagcaa aaaaaataac aaagttgctt tacaatatgc cagctccact atttttatct
      361 gctgtaaaag ttggaagctt cgttgaagtg aaaatgccag ggttaatggc tgctttgaag
      421 cgagtatatt agttaatgga ggaaaatata atgaaaagaa ttttgcattt tcagggacga
      481 atgggtttag gtggagcaga gtctttcatg atgaatttat atcgtaaaat tgatcgtaca
      541 aactatcaat ttgatttttt gatttatgaa gattatgcag atgttcaaga ctatcattca
      601 gagattgaaa gattaggagg ccgtattttt gttgtaccta atcctaagaa aaatattttt
      661 aaatatttaa tagaagtgaa taagttacta aaaaaagaat ctttttctat agttcacaat
      721 caggtttatt ttggcggggg aattaattta tggttagcca aaaagaatgg aatcagacag
      781 agaattgctc atagccatgc aactgaagat ggaaaatctc aaaatattgt aatgaatgta
      841 ttgcgtaaat ttttaaccaa attgttgtta caaaatgcaa cagactacct tgccgttagt
      901 caagaagctg gtgaaagtct gtttcaaaac cacccctttg agatagttca taatggtatt
      961 gatttagagc tttatagtaa aaattcagaa gctaaggtta ataagagaaa agaattggat
     1021 atctcaatga gtacttttgt tgtgggaaac attggacgat tagagaaaca aaaaaatcaa
     1081 tcttatttgt tagagatttt tttagaaata ctaaaaatga attctgattc ttacttgatt
     1141 ttagtaggtg aagggtcact tagaaataaa ctgaaagcca aagcagtaga attggatata
     1201 gagaaaagag taaaattttt aggagaaaga aacgatattc ccgagttatt atctgtgatg
     1261 gatgtttttg taatgccttc tctttatgaa ggtctgccaa tggttggatt agaagcacaa
     1321 gcagcaaaat taaaattagt cttagcagat actatttcca aagatacaaa gttaacaaat
     1381 aatgtggatt ttgtatcatt agatgaaaca ccacaagaat gggcaaaaaa agtaataaaa
     1441 gagccgtatg ctaataaaat aacacaagaa ctaaagagtt atgatgtttc gtacactact
     1501 gaacaaat
//
LOCUS       DAYYHX010000310         1492 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000310 DAYYHX010000000
VERSION     DAYYHX010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.314"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHA6Y_002864"
     CDS             131..505
                     /locus_tag="ACHA6Y_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8988882.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHA6Y_002865"
     CDS             complement(619..1248)
                     /locus_tag="ACHA6Y_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8988883.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHA6Y_002866"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHA6Y_002866"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          507 a          226 c          261 g          498 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aa
//
LOCUS       DAYYHX010000311         1491 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000311 DAYYHX010000000
VERSION     DAYYHX010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.315"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(439..1053)
                     /locus_tag="ACHA6Y_002867"
     CDS             complement(439..1053)
                     /locus_tag="ACHA6Y_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8988884.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          485 a          279 c          247 g          480 t
ORIGIN      
        1 tttgtgtatt ttatttctat tagaaaaagg tatcctgttg gacaattttg ttatatcagg
       61 taaacttcat tgcaataatg ataacaaatg tactataatt actttgtaaa gagttttggg
      121 gaagactctt tcatcaacgt gtattaagag acaagaaaga atccatatgg attctttctt
      181 gtctcttttt ccactacttt ttatttttct gacactacat attcttataa ctcatttctc
      241 ataactgtga aacaacttat aaaaataaca aactttaaac aaaacaagct taaaatcaaa
      301 gatgatccat cttaaaataa ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata
      361 atcgttttct ttttttcaaa cgctccattt ttatgtggaa cattattttt ttaaggaact
      421 ttgtcaacat gtggataact attttccact tgtgtcctgg actagctgtt ttgctgtaaa
      481 atacaaaact tgctgcagca aaaagatcgt tgcaagagag gctgttccgc agatcgctgg
      541 atctagattt ggcaaagtca agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa
      601 atcagcaaat tcaaataatc gcaaaccact gctatgttgc gatttgatac ttcgtgacaa
      661 atcaaatcca gtaacaatga ttaatggatg accattttct ttgatccact gagctagttc
      721 gatgattgcc gggtttcttc cttcattcga aagaagaaga aaaatgtctt ctgatttgac
      781 agaaagctga tcaataattg cctgtcctgc accttctagc gtttcataga cacctccggc
      841 tggattttca atcaatatac ttggtacaag atccggggtt tgatcccaaa attcaaatgc
      901 tacaccactt agtgtgcggc tagcatatat ctgcaatcta ccaccttgat taatttgttt
      961 cgcgatccaa gtgccagctt gaagcatcag cggctcttct atccttaatt tctcttccaa
     1021 ttggtgataa tagtcaaaat aacataaact catttatctc tcccgcctag ctatacttga
     1081 catgaataga aacagctgcc taatctaagg aagtacataa aaaaacagta gttagacagt
     1141 aacacatgtg gtctttgctg tttcaaacac aaaaaaggca ttgaacgaat acgcccaata
     1201 ccttgacata gcaaataaaa caacgtgcag accatggaaa atatctacat aggggtagaa
     1261 ttttcaccct agattaggta caataaatta ggtcgatgca ctgcattgac tatggcaaaa
     1321 tgatcggtca gggtgtatgt gtgtgactgg tcattccctg attcttagcg ccaactaagg
     1381 gtcagggagc ctttttttat tcagctgttt ctatctgaat ttattgtaag taatctctta
     1441 tccaagtgca agcaagaaaa tacaaaaaaa cattggtatg atttatatat a
//
LOCUS       DAYYHX010000312         1478 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000312 DAYYHX010000000
VERSION     DAYYHX010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.316"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..899
                     /locus_tag="ACHA6Y_002868"
     CDS             <1..899
                     /locus_tag="ACHA6Y_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFM8988885.1"
                     /translation="SYSWENKENKEWARELADKLLENGIDAYIDQYDLSLGDRLPQFM
                     EQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAELLSGKNERKFIPIIKEGRA
                     SEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKPALGDIPRHIKGSPLLPIE
                     HNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRVPSQLWKELFVRNWNSPPR
                     YTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIEKSNTQEKKILLEEEQKKE
                     RENERVRSHKESVQKLADELEF"
     gene            complement(896..>1478)
                     /locus_tag="ACHA6Y_002869"
     CDS             complement(896..>1478)
                     /locus_tag="ACHA6Y_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8988886.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT          503 a          229 c          281 g          465 t
ORIGIN      
        1 tatcatattc atgggagaat aaggaaaata aagaatgggc tagagaacta gcagacaaat
       61 tacttgaaaa cggtattgat gcatatatag atcagtatga tttaagttta ggtgatagat
      121 tacctcagtt tatggagcag caaatatccg attcagatta tgttctaatt atatgtacac
      181 cagagtataa aaaaaaggca aataatcgta caagtggtgt tgggtatgaa ggtcatatta
      241 tctcagcaga attgctttct ggaaaaaatg aacgtaaatt tatcccaata attaaagaag
      301 gacgtgcaag cgaagtactt cctacttttc ttgctggaaa gcttggaatt gatttatcag
      361 atagccagca ttatgatgaa aacttagatg atttaattac aactatattt ggtgttaaga
      421 gaaaaccagc attaggtgat atacctaggc atattaaagg cagtccttta ctacctatag
      481 aacataatga aaataaagat attgaaatct taggaattat cactgataaa gtaactgtac
      541 ctaaaatgga tggctcaagg ggttctgcat tatataaaat tccatttaga ttaaatagag
      601 taccaagtca gctttggaaa gagttgtttg ttagaaattg gaattcacca ccacgatata
      661 caacaatgca tagaccagga attgcttctg ttgtagacga tgaaatttta cttaatggaa
      721 caactattga ggaagtccgg gaatatcatc gggaaacttt aaaactttgc attgaaaaaa
      781 gtaatactca agaaaaaaaa atattgctag aggaggaaca aaaaaaagag agagagaatg
      841 agcgagtcag atcacataaa gaaagtgttc agaaattagc agatgaatta gagttctagt
      901 tgatagtcag ttctttctta attcgataca cagtttgtct agttacatta tagtctgttg
      961 ctatttttga aatagcaaca ccattctcct ggtcctgaac gatgcttcta taaacaaggc
     1021 gacgttgagg atcttttgtg ttcgcactgt ataacttagg acgtccttta tagacgccgt
     1081 ttgctttagc aatctttatc ccttgtgcct ggcgtcgttt tgattcagta cgctcttgtt
     1141 cagcaatcat ggccaagatt tgaatgatta ggtctttgat aaatttatcc aggagaggat
     1201 ttccgattgc ttcagccatg atcggtaaat tagtgataat aagtcgcaca tttttcttct
     1261 ttagataatt tactgaatcg ataatctcgt cataattacg tccaagtcga tcgatagcct
     1321 ctacaataaa aatatcttgg tctctaacaa aatctagaac ttcctgaaaa atcggtcgat
     1381 gagtaatagt cgctccagat tgtttctcga tgaaaatttt ttcagcacca aattttctca
     1441 tttcctcgat ttgacgttgt tcattttgat cgatcgat
//
LOCUS       DAYYHX010000313         1476 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000313 DAYYHX010000000
VERSION     DAYYHX010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.317"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            861..1073
                     /locus_tag="ACHA6Y_002870"
     CDS             861..1073
                     /locus_tag="ACHA6Y_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8988887.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1063..1458
                     /locus_tag="ACHA6Y_002871"
     CDS             1063..1458
                     /locus_tag="ACHA6Y_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988888.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
BASE COUNT          515 a          217 c          250 g          494 t
ORIGIN      
        1 ggtgtaaaac agttataaga aatgagtact ctagtatttg tgttacccga ataattactt
       61 gattatattg gtttcccata taattgaagg ctgtcctaat ttttacctac tatatgatca
      121 taattgatta actcttatat atttccgaaa attatcttga agatttctat tggtatggag
      181 aatatccatt tcattgccaa taaggatata aattctcaaa ataacaatcg atgtctgtat
      241 taaacaagcc tagaatggta aatctgattc gccaaaacca ttggattgta tcgatttgct
      301 ttacgcgaac gccacctacc cattttataa aaataaaatg ggcaaaagat ctttcttttt
      361 gtcatacaaa aagaagaaaa aaaagagacg attttcttct tccaaaatcg tccctctttt
      421 ttctaaaacc atggatgaac gaacaaaaaa tctctcgtat tttgtttgtt catccactaa
      481 aaatatgcga acagtatggt ttcattcaat cctgttctta caagtcaaaa gcatcgaatc
      541 gtccttggac gattcaacaa aaaaagtagt cgtttttgga tgactactaa gttctaaaca
      601 ttcattaccc aaaatcaatt tggtctaaca tcgtttctcg actggcttga tctaatcggc
      661 tcttgacagg gctttttctt ttctgttgat agcagataag aggaaataag aataagtgtg
      721 ttcatgtgtc ataaaaacag taactaaaac attaaaatca aaatttatat ggaaagcttc
      781 cttgacattt tgtgctcgca ggagtattat aaaagcacga aaggaaagta aactttccat
      841 tttttagaaa ggagcatgtt gtgaaaaatc gtttggaaga attacgaaaa caaagaggaa
      901 ttaaacaaga agatttagct tctgctcttg aagtatctcg tcaaacaata ggttctttag
      961 aaaatggacg ttataatccc tctattatat tggcatttaa aattgcaagg tactttcaaa
     1021 tgagcattga agaaattttt atctatgagg aggaaagtga agatgaaaaa taagggtcag
     1081 atttggtata tcggttatat tgtttcgttg tttttgattg tgacaatttt ggttacagat
     1141 tttcctaaaa tggtagatat tggacttgct attttattca gtgccgtttt tggcgtttct
     1201 catgttcaaa ttttgcatga taaaatgatt aaaaatgatg cagactacaa aatcaatgtt
     1261 atggacgaac ggaatatcat aatcaaagaa aaagcgggaa atataacaaa tatgtttaac
     1321 acaatactgt taggggtggc aacagttata tttatttgtt tagactacat cattccagct
     1381 attattgtcg gaagtatcat tgcaatccag ccgattattt taatcattat cagtagtgtg
     1441 cttgagaaaa aaatgtagaa ataaaggttc atacaa
//
LOCUS       DAYYHX010000314         1472 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000314 DAYYHX010000000
VERSION     DAYYHX010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.318"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..361
                     /locus_tag="ACHA6Y_002872"
                     /pseudo
     CDS             86..361
                     /locus_tag="ACHA6Y_002872"
                     /inference="COORDINATES: protein motif:HMM:NF017894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
     gene            388..687
                     /locus_tag="ACHA6Y_002873"
     CDS             388..687
                     /locus_tag="ACHA6Y_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFM8988889.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            861..1412
                     /locus_tag="ACHA6Y_002874"
     CDS             861..1412
                     /locus_tag="ACHA6Y_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFM8988890.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
BASE COUNT          532 a          208 c          306 g          426 t
ORIGIN      
        1 atcttcttta atagaagatt gggctatttt tatggcaaag gagggaaaag aaatgtttca
       61 aaataaagga taacgttatg tcacaaaggg agtaatggac agtttgccgg tggaattaca
      121 agctttatgt tggaatctaa tcgatcaaaa cgttcaaaag caatttcctt tagactatct
      181 tcaaattttt gaattttcta cggaaaaagg aaatcaaaag ttggttcatc gtcaagaaga
      241 accggaagaa agaaaagagt atctgatttc acctaagctt cgtttgaaat cagtgtctca
      301 aaaaatatgg gccattgatt cgggtgagta tcaaacgatg ttattaccag aagaatacta
      361 gtatctaaaa gaaaggaacg aaaggacttg ttagaaacgg ctatcgaagt actggaaaag
      421 tgtgcacagc tggtcactgc ttctgaagag tggggctatg aatccgttac gatggaaaaa
      481 gaggaaattg aaatgggtat gcttcctgaa gatgttcatc taccacgttt agtgatgaca
      541 catctttata tttattgcgc accagaagat ggaaaagact atgtagttta tttcattaca
      601 gatattacca gccagcgaga atttgttcgt gggttactgg tagaaggacg attggtttgg
      661 agtcagattg gaggaacgga tgaataagat cgattatttg agaatgattg cgaaatgttt
      721 agaagatatc cttaccgcga ttcatcaaat tctgaagaaa aaaggagaaa gaatatgaaa
      781 tattttcaac tagtattgac acttataacg gcaatggtaa ttgaagtgct tgaacaaaaa
      841 atagcatagg agaaaaagag atgattgaaa aagaattagt gaccacacaa acagaagttt
      901 ggcgagatcc tcatcatacc catcgctatg ttttcaaacg acaatgggcg atcaaaggaa
      961 aagaagcgga agataaatta gcagtagtca ttactattcg tccaacagat acagaaccat
     1021 ttacgaatga tttgagtatg ttattaatcg aaaagaatat ccgacaatta ggatttactg
     1081 gttttattgc tgtgaatctt ttttcgtaca caaaagcgaa aagtcctcgg gtatttcttc
     1141 gaggaaatga tgaacaatct ttggaaatat taacgactgt ttttacagaa aagaaaatca
     1201 ataccattat ttttgcttgt ggttcgatta cagcgacgag tcaagtggct tatgaacaag
     1261 caaagactat ttacgatcaa ttgagtgcga aacaaaagaa gatgaccaaa gtattgataa
     1321 atgcagaagg taagttatcc cacccactaa gtattcatgt tcgtaatgaa tggcaattgg
     1381 cagatagtca attattattt caagaaaaat aacattttat tttctcatgt tacttataag
     1441 tagttttaca tgctttttga ctttaaaaat ac
//
LOCUS       DAYYHX010000315         1430 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000315 DAYYHX010000000
VERSION     DAYYHX010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.319"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            474..725
                     /locus_tag="ACHA6Y_002875"
     CDS             474..725
                     /locus_tag="ACHA6Y_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988891.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="ACHA6Y_002876"
     CDS             759..1037
                     /locus_tag="ACHA6Y_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988892.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
BASE COUNT          509 a          197 c          266 g          458 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt
//
LOCUS       DAYYHX010000316         1424 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000316 DAYYHX010000000
VERSION     DAYYHX010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.320"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACHA6Y_002877"
     CDS             complement(150..1337)
                     /locus_tag="ACHA6Y_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM8988893.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAYYHX010000317         1416 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000317 DAYYHX010000000
VERSION     DAYYHX010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.321"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..228)
                     /locus_tag="ACHA6Y_002878"
     CDS             complement(<1..228)
                     /locus_tag="ACHA6Y_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM8988894.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFS"
     gene            321..670
                     /locus_tag="ACHA6Y_002879"
                     /pseudo
     CDS             321..670
                     /locus_tag="ACHA6Y_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(942..1232)
                     /locus_tag="ACHA6Y_002880"
     CDS             complement(942..1232)
                     /locus_tag="ACHA6Y_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988895.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          445 a          278 c          204 g          489 t
ORIGIN      
        1 agaaaagaac attaagaata gttctataat aattcttgaa ctccatgtct tataatgttc
       61 tatcgaagca cccaaatagc caagttcatc taatctactt gcataaacta cgtcatccgc
      121 tctcatagtc ggcatataca tattaaagag taaaaagata ccccaaatcc ctattagtaa
      181 caagatcagt tctttgttat tcgttatttt tctattatgt ttgttcattt ttatactcct
      241 tcttatattt cctaatagag tataacatag ctttaagcat tacttaaaga taacttaaaa
      301 gttatctttt aaatacttaa ttgttaaata ccttatttga tgcaaaagaa atctacgata
      361 gtcaacaaaa aaattttcat tatatccaga aaaatttttc ttagttaagg acctatggat
      421 tgcagtaatg cagaattcgt cgaataaact gccaaagaca tacaatcata ttgcttttaa
      481 aattgacgaa caagatatag attcgtttgt ctcaaaaatc caaatgttag gtttaactgt
      541 cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca atttactttt acgactatga
      601 taatcacctc ttcgagttac ataccggtac tctccaggaa cgtttaaaat cctacaattc
      661 cactacctga gactagtaat gactagtctc tttttatggc ctctgatccc cccattgagc
      721 gaatactaat agggcttcga attgatctgt tcaatcttta agcaatatcc ctcaaatgag
      781 tttggaaagt ttagtgattc atcacaaata cttaaataca tgtagttata tatagattga
      841 ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc catctttcca
      901 aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt tctagtttat tctttcatct
      961 tgtcggacct tttttcttgg gtatagactt ttctcatatt ctaccacaat tttgggtttc
     1021 cctaacacat gataggatct cacaacaaat tgctttctat tattataata acctgtaacg
     1081 actaacttca tcccttcgtc tacttcagct aggaagttca aactatggga agcaatcaaa
     1141 caattgaccc catttaaact gaaacggatc aacggtctgg cactaacttg caaagtacgg
     1201 atcttatcta ctgttccata ttttttctcc ataaccttca cctcaatacc attatacgaa
     1261 catacattcc ctttgtaaag aagaaactct gttttatctt tttgactttt tgtctattac
     1321 ctttcttaaa atcatattgg cacttcctgc gaagatgaag gacatgtact aaaaaaaaga
     1381 ggcagcagta tagatttaag tagttcagtt ttggag
//
LOCUS       DAYYHX010000318         1415 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000318 DAYYHX010000000
VERSION     DAYYHX010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.322"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..700
                     /locus_tag="ACHA6Y_002881"
     CDS             <1..700
                     /locus_tag="ACHA6Y_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM8988896.1"
                     /translation="HGYKTIICNSQNDPAKEREYIEMLEANQVDGIISSSHNLGIADY
                     ERVRAPIVAFDRNLAPNVSIVSSDNFEGGKLAAKTLQKNGCQNTIMITGNDNTDSPTE
                     LRALGFSFQNPNGKIFKIPNNLSTIRREMEIKSTIINHKPDGIFVSDDLTAILTMKIA
                     HQLKLNIPEDLKIIGYDGTSFIEKFFPQLTTIRQPIDEIASLIVDILLKKIKGEKTNK
                     DYILPISILSGGSI"
     gene            complement(870..>1415)
                     /locus_tag="ACHA6Y_002882"
     CDS             complement(870..>1415)
                     /locus_tag="ACHA6Y_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8988897.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          470 a          238 c          232 g          475 t
ORIGIN      
        1 acatggatat aaaacgatta tttgcaatag tcaaaatgat cctgctaaag agagagaata
       61 cattgaaatg cttgaagcca accaagttga tggcataatc tcatcaagcc ataatcttgg
      121 aattgctgat tatgagcgtg tgagagcacc tattgttgct tttgatagaa atttagctcc
      181 taatgtttcc attgtttctt cagataactt tgaaggggga aaacttgcag ctaagacttt
      241 acaaaaaaat ggttgtcaaa ataccattat gattacagga aatgataaca cggattctcc
      301 cacagaatta cgtgcactag gtttttcttt tcaaaatcca aatggaaaaa ttttcaaaat
      361 tcctaataat ctgtctacta tccgtcgtga aatggaaata aagtcaacta ttataaatca
      421 caaaccagat ggtatttttg tttctgatga tttaacagcc attctcacta tgaaaattgc
      481 tcatcaactt aaacttaata tcccagaaga tttaaaaatt attggatacg atggtacctc
      541 tttcattgag aaatttttcc cacaattgac aacaataaga caaccaattg atgaaattgc
      601 aagtcttatt gttgatattc ttctgaaaaa aattaaaggc gaaaagacaa ataaggatta
      661 tattttacca atctcaatac tttctggagg aagtatttaa cgttaaatta attttttaat
      721 gtctataaaa ttcgttagaa aatacaattt gtagatatcc aattgatttg taaacaaaaa
      781 ggagttgtca acttgtagtg acccccaaaa gttagacttt tttggagtgg agaaatccac
      841 tccttttttc atgctctaaa atgatatttt tacacaactg ttacggttgt tttacgaaat
      901 tggacgggac tttgccagtt taatttctgt tttatccgtt cattattgta gtaatagata
      961 tagctatcga tggctgattt taattcctcg aaacagttat aaactttccc gtgaaaaatc
     1021 tcttgtttta ataagccaaa aaaattctcc ataagtgaat tatctaagca gttccctttc
     1081 cgagacatgc tttgaaagat tttgtactcc ttcaatttgt ttctataagc tttcatttga
     1141 tacgcccaac cttgatctga gtgaaacgta cgacgatagg gacaatcgtt agtggtctgg
     1201 atagcttcct ccaaaccttc cattaccgct aatgcattgg gcttttcaga gatacgatag
     1261 gataaaatct cactattgaa catatccata aaaggatcta gatacaattt cttttgacgg
     1321 ataatgcctt tggcatccac ttcatggtat ttaaattcag ttgtatccgt cgtgattttc
     1381 tgatggcaga tactggtgta gaaacgacga tggat
//
LOCUS       DAYYHX010000319         1386 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000319 DAYYHX010000000
VERSION     DAYYHX010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.323"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..226
                     /locus_tag="ACHA6Y_002883"
     CDS             <1..226
                     /locus_tag="ACHA6Y_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988898.1"
                     /translation="GMKSRGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDV
                     VREKFERQIFKIKSVKNLCKLMNHVINIYP"
     gene            249..1274
                     /locus_tag="ACHA6Y_002884"
     CDS             249..1274
                     /locus_tag="ACHA6Y_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8988899.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
BASE COUNT          477 a          154 c          322 g          433 t
ORIGIN      
        1 tggtatgaag tctagaggct taatatggga tgggatattt ggattaggca aaaggttttt
       61 agatagggat ttttggattt ggtttataat tgcagttgta gttgtgtgca taatttgcat
      121 ggctatcgat gttgtaagag aaaaatttga aagacagatt tttaaaatta agagtgtaaa
      181 gaacctgtgt aaacttatga atcatgttat aaatatatac ccataattaa taaatgagga
      241 gaaaagaagt gaaagttata tcattcggag aattattatt gagacttgca gcacccggtt
      301 attcaaagtt atttcaaaga gatagtttgg agactacatt ctgcggaggg gaagctaatg
      361 tagcggtatc cttagcaata tttggatatc aatcagaatt tataacaaag cttccggatt
      421 cagatgttgg atatgcggca gctaatgctt tgcgttattt tggagtaaat gttgataata
      481 ttatatttgg agagggacgc atgggactgt attatttaga aaaaggagca gctcagagac
      541 catctaaagt aatttatgac agaaaatttt ctgcaatttc gttagctgat agtgatgaat
      601 ttaagtggaa ggagatccta aacgatgcaa attggtttca ttggacaggg ataaatccag
      661 cattgtctga gaatctcttt gagacgtcct tagatgcatg tcaggctgcc aaaaaaagag
      721 gtataacaat ttcttgtgat ttaaattatc gatccaagct ttggacatca gaggaagcaa
      781 aagaaaagat ggaacagata ctaccattgg ttgatatttg tatttgtaat gaagaagatg
      841 cagaaaaagt gctaggaatt aaagctgagc atacaaatat taatagtgga gaattaaata
      901 aagatggcta tgtgcaggtg gctagggaga ttcacaagaa gtttggatgt aggtatgtag
      961 ctacaactct gcgtagaagt tattcggcaa gtagaaatgg ttggagtgca atgttatacg
     1021 aagcagaaaa agataaagcg tatttttcca cagaatacaa catacaaatt gttgatcgcg
     1081 ttggtggtgg agacagcttt tctgctggta taatatatgc gttatgtgaa aatatgtccg
     1141 gacaaaaggc tattgaattt gcgactgcat ctagttgttt aaaacatact atagaaggtg
     1201 attttaatcg aactacaatt gaggatgtat ataatttaat gaatagcggt ggaaatggtc
     1261 gcgtgttgcg ttagtcaaca tataaagata aatcacagta atttttatgg tgattatctg
     1321 tcttagtagt gattagaata acgtgaaaat aaattgttga gcgtttatac tgttttgaaa
     1381 aataaa
//
LOCUS       DAYYHX010000320         1376 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000320 DAYYHX010000000
VERSION     DAYYHX010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.324"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(316..1206)
                     /locus_tag="ACHA6Y_002885"
     CDS             complement(316..1206)
                     /locus_tag="ACHA6Y_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM8988900.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          359 a          294 c          247 g          476 t
ORIGIN      
        1 tacaacaagg tctgatcttt gaaaactaaa aaaatatgcg gcgcacagaa cgcgccgcat
       61 atcggtctaa gttttattca aatttgtctt caccaacacg atttgacgaa gagccaaaaa
      121 aagttacgac agttctatgt cgtagctctt tttttcgaat aaacggtgtt caatcagctg
      181 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctatttttca caattaaagg
      241 aaaaatgtat atgtggggaa acatagttga tttttcctta atagtaacga ctaccgtggc
      301 atgtttatct gctagttatt tgatatttcc ttcgtgatca cgttctactt ccatcattgt
      361 gattggtaaa tagcccaatt ctttgacagg tccttcttgg atttcttctg tcatcatgta
      421 gtctaagaat ttttggactt caggggaagg ttttccgttc gtgtacatat gttcatatga
      481 ccagattccc cagctgttat ctgctacatt ctcttctttt ggttctacac cgtcgatact
      541 tagtgcttgt gtggaatcat cgagatatga gaaagctaaa tagctgattg ctcctggtgt
      601 ttgacttacc agctgccgaa ctgttcctga tgaatcttgt tcttgggact gcacaggggt
      661 agcaccatcc agtccccatt tttcgaatgt cgcgcgggta ccgctgccgt ttgcacggtt
      721 cacgacgttg attttttgat ctttcccgcc aacttccttc cagttagtga tttttcctgt
      781 aaagatatca atcaattcct gtttggtgat atctttgaca tctgtatctt tattgacgat
      841 tggtgccatc ccgaccactg ctactttatg atccactaat tttgaagcat caactccgtc
      901 tctttcttct gcaaatacat cagagtttcc gatagtaact gctccagctt cgacttggct
      961 caaaccagtt ccgcttcctc ctccttgtac agagatttga taatttggat tttcttgtac
     1021 aaagctttct tgtgctgcat cgactaacgg ctgtaatgct gtcgaaccta ccgcaacgat
     1081 ttttacttgt tcatttgagt cacttgaatt attttgtgtg gcagagccat ctgtatttcc
     1141 tgatcctgag ccgcatccgg ctaaaagaat tcccatcata cctagagtaa acaatacttt
     1201 tttcatttta ttgctccttt cttatttaca actaaagttt atcagtcgac tgtaaatccc
     1261 tcgtatggtt gctgtaaaaa tatagttaag ttatgtaaaa atcatgtaaa cgtttttcgc
     1321 attctttaca taataataaa aaaagcatat gagaaacatg cattttacgc agctca
//
LOCUS       DAYYHX010000321         1366 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000321 DAYYHX010000000
VERSION     DAYYHX010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.325"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..1338
                     /locus_tag="ACHA6Y_002886"
     CDS             106..1338
                     /locus_tag="ACHA6Y_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8988901.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT          447 a          258 c          301 g          360 t
ORIGIN      
        1 aaaaaataac ctcctttttt tatttaaagc ctcattgttt gaatcataat taaatggtat
       61 catgctaata gttaccagaa aaaatggtga ggaggaattt ttattatgtt atctgatttt
      121 aatgaacttc taaaaaaata tgcacgctta attgcagaaa ctggcgtagc cacagaaaaa
      181 ggacacacag ttgttttaca gatcagcgtg gaacaggcac cccttgctcg attgatcaca
      241 caagaagctt ataaactagg agccgctgag gttatcgttc aatggactga cgaccagatc
      301 caaagagaat ttcttttaca tgctgcaacg gatcgtattg agaatgtacc acaatctaaa
      361 atcgatcaag cggatgaatg gcttgaaaaa ggagcaagca ggatcagcgt tgtttctgcc
      421 gatcccgatg catttgctgg tgtagatagc catcgagtag cgtcttatca atcagcgaat
      481 ggcaaagcac taatgaattt acgcaaggca actcaagcaa ataaagtcag ttggactgtc
      541 gtggctgcgg ctggtaaaca atgggcagca aaagtcttcc cagaactgcc agaagaaaaa
      601 caagttgagg cattatggga tcagattttc aaaacgactc gtgtctatga agaagaccct
      661 gtcttagcat ggaagaagca tgatgaaaaa ttagcaaaaa aagcagaaga attgaatcga
      721 gaacaatttt ctgcccttca ctatactgct cccggtacag atatcattat cggattgcct
      781 aaaaaccatc tttgggaagg cgctggaagc tataatgctc gtggagaaaa attcatggca
      841 aatatgccga cggaagaagt gttcacagca cctgacagtc atcgagtaga tggttacatt
      901 tcaagtacaa agccactgag ttatgctggc accatcatct cgggtatgaa atttactttt
      961 aaagatggaa aagttgttga tttttctgcc gaacaaggag aagatgtctt agcaaaactg
     1021 cttgatacag atgaaggtgc gcgtcgttta ggagaagtcg cacttgtacc tgacccttca
     1081 cctatttctc aatctggcat catctttttc aatacattat ttgatgaaaa cgcatcgaat
     1141 cacttagcac taggttctgc ttatgccttc agtgtgaaag gcggtacaga aatgtccgat
     1201 gaagaattag cagaagctgg attgaataga agtcaaaccc acgtagattt catggtagga
     1261 tctgacaaga tggatatcga cggaatccgt gaagatggct ctacagtacc gattttccga
     1321 aatggcgatt gggcttgagt atactttcgc acacatgcaa aaaata
//
LOCUS       DAYYHX010000322         1337 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000322 DAYYHX010000000
VERSION     DAYYHX010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.326"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1337
                     /locus_tag="ACHA6Y_002887"
     rRNA            <1..1337
                     /locus_tag="ACHA6Y_002887"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DAYYHX010000323         1331 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000323 DAYYHX010000000
VERSION     DAYYHX010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.327"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..763
                     /locus_tag="ACHA6Y_002888"
     CDS             80..763
                     /locus_tag="ACHA6Y_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8988902.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
     gene            complement(800..1297)
                     /locus_tag="ACHA6Y_002889"
     CDS             complement(800..1297)
                     /locus_tag="ACHA6Y_002889"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8988903.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT          419 a          235 c          223 g          454 t
ORIGIN      
        1 aatcaacaaa aaaacgcata tagtagtctc aaaactccca agaaatgagg actatttaac
       61 aaactacgat ggttacgtcg tgacaactga acattttgac ttagccaatc ctgttactaa
      121 agtcgatgat atccccgatt atgaaatgta cagtcaaaca atcgatagct taaataagcg
      181 ttttggcaat cgtgttttaa aaggcatcaa aatcggttac atcgcttctg aaaaggatcg
      241 gatcatcgac tatttagccg ataaagatta cgatttaaaa ttactcagtg tgcatcataa
      301 tgggcaattt gattatttag atgacgaagt caaagatatg gatcctgcta ttgtgattcc
      361 tcagtatttt gcacaacttt cagaagcact agttgttata gaagcagatg tctttgctca
      421 ttttgactat ggttttcggg tatttggtct atccgttgct gaatttaaac aatatgaagc
      481 gcagttttta ccgattctag atcaagtaat caaaaataaa ttggcattcg aattaaatgc
      541 taagagcgct tacttgtatg acaatttagc gttatatgaa tatgtcatcg atttatatct
      601 ttcacgtggt ggtaccttgt ttagtgttgg atcagatggg cattaccttg aacatttccg
      661 tttccatttc gatgatcttt ttgctctgtt aaaagcaaat ggtgtgacgg aattagcgat
      721 ttatcaaaaa ggaaagcgaa ttatggttcc attaccagtc taaaaaaacg tcctcagcat
      781 caaaagtgag gacgtttttt tagatttctt tttctaaaat cggacaaata cgatcttgtt
      841 ttcctaaagg aaaatgtttg atcccgattt ttttaaatcc atgacgttca taaaaacgaa
      901 tacctgcatg atttttttca taaacagtca aacgtaaata acgtttttgt ttcgttttag
      961 cataacgatt cgcttcatcg agtaaccgtt gccctaaccc tttgttttga taatctttga
     1021 gcagatagag ccgttgaatc tctatagcat ctttttcatc attgactttc atataaccaa
     1081 tgagtatttg atgatcctta ataccaataa atttactggt tgactgtgcc aattctactg
     1141 ccaatatttc agttgtcaat tgtgtctgta aatatccttg catatcaagt ggatctgcgc
     1201 catcgataaa cgattcataa aaactgacaa cacttacaac ttgtaaagca tctagatcat
     1261 cataatctaa atacgctagt gtgatttgat tgttcatcta actcctcctt ttgacatagt
     1321 atactataaa t
//
LOCUS       DAYYHX010000324         1314 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000324 DAYYHX010000000
VERSION     DAYYHX010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.328"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1166
                     /locus_tag="ACHA6Y_002890"
     CDS             <1..1166
                     /locus_tag="ACHA6Y_002890"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8988904.1"
                     /translation="KGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQIV
                     AHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKLTQ
                     QPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDLLY
                     MRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQPY
                     EKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNELGT
                     GNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQGNI
                     DSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          473 a          242 c          219 g          380 t
ORIGIN      
        1 aaaaaggaaa acatgctaaa aaaagaaaat attcattttt aatgatcgtt gtcgtgttgt
       61 ttagccttag tgcgttctct acaggaacta ttcttggatg gcaattttac aataatgaac
      121 actctcagat agttgcccat gctcaatcag ctcaaaaaaa gttgatactg actaccaatg
      181 aacaacagct cgttgaaaaa atcgatcaac tattaagaaa aaatgattat attggctcaa
      241 tttacgtaaa gaaaaataat cgcatcatca tggaaaaagg ctatggctat gcgaataaac
      301 tcacgcaaca accaaatgat ccttctcttt attatcagat tggttcggtt caaaaagcat
      361 tgactactct tttgatttta aaacaagtcg agcgtgggca cctttcttta gatactaagc
      421 tggctcaatt ctatccacaa attgctggca gccagcaaat tactatccgt gacttgctgt
      481 atatgcgctc aggcttgcac cgaactgctt ctccaaccat accgatgact gatgaagaag
      541 tcgttcaatt tgcgattcag cacttaaaat ttacagatta tcagacctct cattatgatt
      601 cattgaactt taccatattg actggaattt tgatcaaatt aacaaatcaa ccgtatgaaa
      661 aattattgac cgatgatatc atcgaacctt taggattaca gcatacaaaa ttttatgacc
      721 aagtcaaaaa ttcatccaat catgcactat cctatcaaat gtctaaagag gatgattact
      781 ttaaaccact gacagaatct gaaacagaga tccataacga attagggact ggtaatatca
      841 gtatgtccgt ctatgattta aatacgctgt ttacacaagt tttatctgga aaactagttt
      901 ccaaagactt actcttctca ttatggcgtg aaaatgcgaa tggacatcca tatagtggtg
      961 gggtatatag cggagataat tatatcctgt cgcaaggaaa tatcgactct tttgatacat
     1021 ctgctatatt tagcaaagat tcacaggatg ctatcatcat ggaaagcaat ataaaagctg
     1081 acaaaactat caaactgcct actgctgatc taagaaacca aatctatgac ctgatggaag
     1141 gaactactaa gttggcaaat agctgatatg atgaaaagat agcaaatact acttctttga
     1201 atagcatttg cttaaaaaaa gtactcgact aaacgtagga aagagtgtgg gacaaatcta
     1261 aaaatcaatt tttgctacag actttaattc caaataaaca gaacagaaag aggt
//
LOCUS       DAYYHX010000325         1311 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000325 DAYYHX010000000
VERSION     DAYYHX010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.329"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..>1311
                     /locus_tag="ACHA6Y_002891"
     CDS             128..>1311
                     /locus_tag="ACHA6Y_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8988905.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LHT"
BASE COUNT          411 a          263 c          235 g          402 t
ORIGIN      
        1 ctctataatt tctaggactg atggaaattt tctatctgtc ctagtttttt tgtaaaaaaa
       61 caaaaagaca tacaaaattt ccggtagaat agagtctcct acaacatcat tctgggggaa
      121 ttcttgtatg tccatgaatc attttatcaa acaactcttt actattaaag actcaaatat
      181 ccaaattcaa tcctttgaag aaacttttta taaaagcaga aaaagtatcg ttattgaggg
      241 aactctgatc cgtacctaca gacgttgtcc gtgttgtaaa gattctacaa agcaaatcgt
      301 taaaaatggc aagaagatct ctatgattct cctgaatcgt agcggtaata aacggaccta
      361 tcttcggttg aagaaacagc gatacctctg ccgagcatgt aaaaaatatt ttaccgctcg
      421 gacgtatctc gttaccccat tctgctttat ttctaagcag attcattaca agatcttaga
      481 agaattgaca gagcgtcaat ctataaaagc aattggtaaa cattgcgatg tttctgtgac
      541 aactgttcag cggacattgt ccgccttgtc atcagatatc cgtcagaatc ttgagtattt
      601 acctcggtgt ctattattcg atgagtttcg ttctctttct acatggcacg gtaaattttc
      661 tttttcttgt atggacggtg agaccgggaa actgtttgag atcttaccta gccgccgtaa
      721 aaaagatctc gttgcttttt tcatgcgttt ttctctcaaa gcgaggttgg gtgtacatta
      781 catcgtaacg gatatgaacg ctccttattt ctcactagtt aaagagtgtt ttccaaatgc
      841 acaagtgatc gtcgatcggt ttcacattgt tcaacatctg aaccgcttct tcgatgcggt
      901 tcgaaaacgt gtgatgaaaa cacttgatca aaaagaccca atacaagcta aacagtatcg
      961 ccaattgaaa tccttattca aattactttt aaaatcagag gatcgtttag attacaatac
     1021 actaacgaaa agacgcaatt tcaattggag actattgact gaaacagaag tagttgaccg
     1081 ccttctaaag atttctcctg aacttaaaac agcttatcgt tactaccaag acctgcttac
     1141 tgcgtatcag gaaaaagatc cagatacttt tttccagctg cttcgagcaa tgcctggaga
     1201 agttccattg gaactgcacc atatcaaaaa agcctttttc aaatacgaga aagggattcg
     1261 gctggcatta ctgaagaagt attcgaatgc acgactggaa aatctccata c
//
LOCUS       DAYYHX010000326         1307 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000326 DAYYHX010000000
VERSION     DAYYHX010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.330"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..459)
                     /locus_tag="ACHA6Y_002892"
     CDS             complement(<1..459)
                     /locus_tag="ACHA6Y_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8988906.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLF"
     gene            complement(592..>1307)
                     /locus_tag="ACHA6Y_002893"
     CDS             complement(592..>1307)
                     /locus_tag="ACHA6Y_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8988907.1"
                     /translation="KKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLLKAML
                     NIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKVFQRI
                     KNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEPFVGI
                     DLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVESVFNE
                     ENLKKAYGDTIFIGEGGTA"
BASE COUNT          457 a          249 c          153 g          448 t
ORIGIN      
        1 aaatagaaca actgtaacca acactaaaac agaaacgcca attgtaatcc acttatcaat
       61 atcttgtaca gctaaaatat ttccaaataa gatatgaaat aaatccgttg agctatttgc
      121 aactcctata aggataactc ctaatgctaa aaaagaactg aaagtaatac caattgctgt
      181 atctcctttt attacactat tgctttttat aaaagtaata atgatagaag ctaatagacc
      241 aaatacaatc gcaccaataa aaaaattaat acctaaaata taagaaattg ctactcctgg
      301 caaaacagca tgagaaatag catcacccat aagagacatt cctcttaaaa taataaaaca
      361 acctactgtt ccagaaacaa tccctataac tacagatgta attagagcat tctgtaaaaa
      421 gtgaaagtca tacagaccat taataaaatt ttgaagcata ttattcccct tgtatattag
      481 agttagggtt ttaaccttcc ggttttgatt ggataaccct atgaaccatc gttattttaa
      541 tttggtatta actcatttct tttgagataa tatatttatt ggattgagag gctaagccgt
      601 tcccccctct ccaataaata tagtatctcc ataggctttt tttaaattct cttcattaaa
      661 tacagattct acacttccgt gagcaattag atttcgattc aaaagaataa tattatcaaa
      721 atacttcttt accttgctta aatcatgatg cacaattagc actgtttttc cctgatgttt
      781 caattctttt aacgtatcca taattattcg ttcactaact aagtctatcc ctacaaatgg
      841 ttcatctaaa aatatgaaat ccgcattttg agcaagacat cttgctaaaa gtacacgttg
      901 aaattgtcct ccagataatt ctcctatttg atgattagaa tattttccca tatcgacctt
      961 ttccaaagcc tttgaaactc tttgccattc tgcattttta attctttgaa aaactttcat
     1021 tccggcatat gttcccatag atacacattc ttttactttt attggaaaag taaaatcaat
     1081 atcagctttt tgctctacat atgcaatctt caccagtttt ttattgatat cttcattatt
     1141 aatggagacc tctccctgat gagggataat atttaacatt gctttaagca atgtagattt
     1201 tccagcacca tttggaccaa taatacctgt taaagcacct tcttgtattt ttaaagaaat
     1261 ctcgttcaat gctaaaaaat cgttataaca aacattaagc tttttca
//
LOCUS       DAYYHX010000327         1294 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000327 DAYYHX010000000
VERSION     DAYYHX010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.331"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACHA6Y_002894"
     CDS             <1..1244
                     /locus_tag="ACHA6Y_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM8988908.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAYYHX010000328         1293 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000328 DAYYHX010000000
VERSION     DAYYHX010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.332"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..254
                     /locus_tag="ACHA6Y_002895"
     CDS             <1..254
                     /locus_tag="ACHA6Y_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8988909.1"
                     /translation="RYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRD
                     YLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            259..1206
                     /locus_tag="ACHA6Y_002896"
     CDS             259..1206
                     /locus_tag="ACHA6Y_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8988910.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          426 a          188 c          268 g          411 t
ORIGIN      
        1 caagatacac atctaggcaa cagacacctg aagtttttga aatgaatgcc tcaatgtatt
       61 cttttaaacc tgaatttcta aaaaaaagta attatatttt tgatgggcaa tgtgggataa
      121 taaaaatgag agattatcta attttagata ttgatagtga agaagatttt aaatggttag
      181 aatttttgtt tcctaaattt ttagaagatt cttcggagct tcaagatatc tataattatt
      241 tatatcggag gtaaaaggat gagctgttat cttatcaccg gtggggctgg cttcattggt
      301 tctaccttag ccaatcattt aggagaagaa catgtggtaa tcgtcgtcga tgacttatca
      361 atgggaaaag tagaaaatct tgatatgacg aaaaatatca cttttattga gggagatgtc
      421 gctgagaaat ccttgatgga agagatcatg aaagctaata aatttgatta tatctttcat
      481 ttagcagcag ttgctagtgt ggcagattca gttgcccgac cagtagaaac acatcgtgtg
      541 aattttgaaa gtgtattaat gattttagaa ttgatccgca agtatcaacc tgatttaaaa
      601 cggatcgtgt ttagttcttc agcggctgtt tatggggatg aaccgacttt accaaaaaaa
      661 gaagagtccg tgattcgccc attgacgcct tatgcggttg ataaattcgc tgcagaacaa
      721 tatgtgctag attattgtca tttatatgat gtacccggta gcgcagttcg ttttttcaat
      781 gtctatggtc caaatcaaaa tccgaattct ccatacagtg gtgtgatttc tattctcgta
      841 gatcgctata agaaacaaat cgtaggagaa gcaacttcat ttacattatt tggtgatgga
      901 agccagtcta gagactttgt ttatatagat gatgtaatcc aagcattact tttagttgcg
      961 aatgaggaag ctgcactggg gaaacaattc aatgttggaa cgggtaaagc aaccacactt
     1021 ttggaattga ttcatgcaat caatgaaatt ctcggtgtag aattgacact tgaatacaaa
     1081 gaagagcgtt cgggtgatat ccatgactct ctagcagata ttactaagat ccagtcaatt
     1141 ggctatcaac cgaaatatgg tattttaagt ggaatgacta attatttgaa aactgaaatc
     1201 aattaaaaca tactaagata agattagagg gtaaattcag gctctttgtc aataaggact
     1261 gatgagtttt gtaaaatcaa atctgggata gaa
//
LOCUS       DAYYHX010000329         1250 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000329 DAYYHX010000000
VERSION     DAYYHX010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.333"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..137
                     /locus_tag="ACHA6Y_002897"
     tRNA            64..137
                     /locus_tag="ACHA6Y_002897"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:98..100,aa:Ile,seq:cat)
     gene            683..>1250
                     /locus_tag="ACHA6Y_002898"
     CDS             683..>1250
                     /locus_tag="ACHA6Y_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFM8988911.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSN"
BASE COUNT          404 a          216 c          231 g          399 t
ORIGIN      
        1 gaaacccatt attatctagt ctatgtccat tctttttgtt tgcagtagtt gtgatggctt
       61 tccgtactct tagctcagtt ggttagagca gacggctcat aaccgtccgg tcgtaggctc
      121 gagtcctaca gggtacatac tctaagtgat ttaaattgcg aataaaatat aaaaatgact
      181 ccccttttga ctcccccaag cagtatatgg atgtctctaa aagttgtttt tttagataat
      241 aaaaagctga taataagcga aaaacactta aaatcagctt taaaccctat tctcgaaatc
      301 cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg taccttcctt atatcaacga
      361 ttagaggttg tgaagaagct gtcctgcatc aagtaataag aacggccaaa caaaaatagc
      421 ttttcctatt ttttcgtttg attgggctta ttaccgcaga tgcaagcttt tgaacaccgt
      481 ttgttcggaa taaggggctg tgacaagact tttgtcacag ccccttattg ttgtactttt
      541 aacctacaat attttttgat tccccctgag tactattttt tataaatgct agtaaatcaa
      601 tcttctttgg cctttttttg ataaaaaaaa tactagatgt aagataaaag tgtaaaaacg
      661 atttgtttta ggaggggaaa cgatgtgtac cgcaatcact tatgcaacga aagaccatta
      721 ttttggaagg aattttgatt atgagatgtc ttataatgaa gtggtcacta ttactccaag
      781 aaattatcgt ttcgatttta gaaaggtaaa aaatttagat aaacattatg caatgatagg
      841 aatagcagct ggggtatcca actatccact ctattatgag gctacaaatg aaaaagggtt
      901 gagtatggca ggactgaatt ttcctggcaa tgctgattat aaagaattac aagaaggaaa
      961 agataacgtt gcaccatttg agtttattcc atggatacta gggcaatgct caaccataga
     1021 cgaagctaaa gaattattgg ctactatcaa tctagtcaat atcgatttta gtgaaaaact
     1081 acccttgtca cctttacact ggttattagc tgataaagaa aaatcgatag taattgaaag
     1141 tatgaaagat ggacttcatt tatatgataa tcctgtcggt gtgttgacaa ataatccgcc
     1201 atttgattat caattattta atctgaacaa ttatcgctcg ctatcaaatg
//
LOCUS       DAYYHX010000330         1242 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000330 DAYYHX010000000
VERSION     DAYYHX010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.334"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1242
                     /locus_tag="ACHA6Y_002899"
     CDS             82..>1242
                     /locus_tag="ACHA6Y_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM8988912.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIY"
BASE COUNT          413 a          243 c          239 g          347 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct at
//
LOCUS       DAYYHX010000331         1242 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000331 DAYYHX010000000
VERSION     DAYYHX010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.335"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACHA6Y_002900"
     CDS             complement(<1..210)
                     /locus_tag="ACHA6Y_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8988913.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
     gene            complement(309..623)
                     /locus_tag="ACHA6Y_002901"
     CDS             complement(309..623)
                     /locus_tag="ACHA6Y_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988914.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(691..1185)
                     /locus_tag="ACHA6Y_002902"
     CDS             complement(691..1185)
                     /locus_tag="ACHA6Y_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988915.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
BASE COUNT          390 a          236 c          143 g          473 t
ORIGIN      
        1 taaatcagat acataagccc atgtacccgt tcgttgtaaa taaagttctt cttgtaacaa
       61 ctcccatttt ctcttagaaa tcataatgac gccatcgttc aatccatttt tagaatccct
      121 agtaatttct atttcagaat ttgaatgaac tacttcttgc aacactttac ctatattttc
      181 tttgaagttt gttggagtaa tagtatccaa aatgaatacc tctcttctct atacctctat
      241 tatgaagtgc ccataaaata aagcaagcgt tgaggaataa ttctatcaac acttgcttta
      301 ttaagctttc attttgtagt ttgactaaaa ccaataatct tatttctaaa ctctcgaaaa
      361 acttcttgtg gttgataata gtctggatct atttcaaatg tttgtgagaa taattcattg
      421 aatttttcta ctgaaataat gattttacca ttcttctctc gactgttttc tattatttct
      481 gcttctaatt caaataaatc tagtgacgaa ctaaaatcat aaatcatgtc atattgaata
      541 aaaaaatcaa gaaaaagttg ctcaaactct tccttattta tcactaatgt ttcaaacgga
      601 acaactttat tttcctcagc cattctccca gttccatttt caaaaatcac aaaactgata
      661 ttaccttaaa aactactaac aaaataaaac ttataaagta aatggcaacc caactatgta
      721 accattctct ctacgtacta cgatttggta gtcctgcctt tcaatcggta attcataata
      781 gtaatttact gtttcttctt tgccactttc aatatactct ggattatcca atatcatatc
      841 atactcataa agttcttcac catccttatt ttcaatcgac cattgttgtg gacgtatcgg
      901 attttctgta tcatttataa gtgtataggt cacttgtaat acacgatcat gttgtgcaaa
      961 ttcatttctt ttttcggtca cttcaatatt tttaaatgtt agttcaaccc catctgcctg
     1021 ataagtttca cctattttta aatcttttgt tacaattttt attggctttt cttctggaaa
     1081 atgtctggct ttaattgtta gataacttcc aaaaagaatc aatcctagta gcactaaaag
     1141 aattccttta acgagccact gaaacgggaa gtaaacttta aacatctttt ttctcctccg
     1201 ttttgtattt gttttttaga gaatacgata acttttgatc aa
//
LOCUS       DAYYHX010000332         1241 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000332 DAYYHX010000000
VERSION     DAYYHX010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.336"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..894
                     /gene="scrK"
                     /locus_tag="ACHA6Y_002903"
     CDS             22..894
                     /gene="scrK"
                     /locus_tag="ACHA6Y_002903"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8988916.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          374 a          228 c          301 g          338 t
ORIGIN      
        1 aaaataaagg gagaaatgtc tatgctatac ggtgcaattg aagcaggcgg cacaaaattt
       61 gtgtgtgcca ttggcgatga agaaatgacg atcaaggaaa gagtgagctt tccgacaacg
      121 acacctgagg aaacaatgcc tttagtcatt gacttcttca aacaatacca agcagattta
      181 gcagggattg ggattggctc atttgggcct atcgatatcc atcgcgattc cgccacttat
      241 gggtacatta cctcgacccc gaaattggca tggcaaaatt ttgatttcat cggtaccatg
      301 aaaaaagaat ttccgatccc gatttcttgg acaacagatg tcaatgcggc tgcctatggt
      361 gagtatgtct tcggcagtgg caaaggattg agcagtgtcg tttactatac gatcggtacg
      421 ggtgttggcg gcggtgcctt acaagaaggg cgctttatcg aagggtttag tcatccagaa
      481 atggggcata tgctcgtcgt ccctcatcca aaagatgact ttgctggcag ctgtccattt
      541 catggcaact gtttagaagg gatggccgct ggacccgcca tcgaaaaccg cttagggaaa
      601 aaaggacaag aggttgcaga agatgatcct tattgggaaa tcgaagcctc ctatattgct
      661 caatgtgcct acaatacaac cttgatgttt tctccagatg tgatcatttt tggcggtggt
      721 gtgatgaaac aacgccatat gctgcaaaac gtccacgacg catttgcaaa attggtcaat
      781 gggtatgtcg aaacacctga gttaagcaaa tatatcgtga ccccagcatt agaagacaac
      841 gctggaacgc tcggttgttt ggcattggct cgagaagcgg tcttacattc ataaacgaaa
      901 aaaggctctt cgacatttag tgttggtaga gaaagataca actgaaaatt tagtcataac
      961 cagttaggca gagtgcttaa ctggtttttg ttttacttgg aagatcaagc cttgaatgag
     1021 ataaattctt gctcgaaagt tatggaaatt tcgatagccg taggccactt gtattgtgca
     1081 atagtttttt acaaaaaaaa gggggtgcgg atattttttt ggaccttttt aacaaatagg
     1141 ttctgttgca aagttttaaa tctactatca gataaggtag aataatagaa aaagatagca
     1201 ggaggaatga cgatgagtca ttttaaagga aagcaatttc a
//
LOCUS       DAYYHX010000333         1229 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000333 DAYYHX010000000
VERSION     DAYYHX010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.337"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..366
                     /locus_tag="ACHA6Y_002904"
     CDS             10..366
                     /locus_tag="ACHA6Y_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8988917.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            356..622
                     /locus_tag="ACHA6Y_002905"
     CDS             356..622
                     /locus_tag="ACHA6Y_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8988918.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            781..1059
                     /locus_tag="ACHA6Y_002906"
     CDS             781..1059
                     /locus_tag="ACHA6Y_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988919.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(1056..>1229)
                     /locus_tag="ACHA6Y_002907"
     CDS             complement(1056..>1229)
                     /locus_tag="ACHA6Y_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8988920.1"
                     /translation="HRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFS
                     VCTEIRILLGIPA"
BASE COUNT          471 a          168 c          246 g          344 t
ORIGIN      
        1 taattgggta tggaaaaaat atcttttgaa acatttaaaa gacccaatgg tcatgatgaa
       61 tttttagaat ggttggaaac attaccaaaa aaagatagtg cgaagctttt acgtacaata
      121 gaagaaacag aaaagaatgg gatgctagta gctcaacggt tgaaatgggt gaaaaaatta
      181 gacactaatt tgtacgaatt aagaagtaag gtaggatcaa acatccaaag agccatctat
      241 ttccatgtgg acaatggaag gtatgtcatt actcacggat ttactaaaaa aacggagaaa
      301 acaccaataa ctgaactcaa gcatgcaaaa gaacttagag aggagtggtt cacaaatgat
      361 gattaaaaaa ttgattgaag aaaagaaaaa agataaagat tttgcagaag catatgaccg
      421 tgaaggaaga aaactcgaaa ctgcggttgc tctttatcat gctcgggagg aagcagggct
      481 aactcaagcg gagcttgcac aaaaagcaca tacaactcaa tctactattg ctcgaattga
      541 gagaggggat aacgtatctt tcgataaact tgatgatatc gccagggcat taggtaaaaa
      601 gctggtagta gaatttgctt aaaagcgagt tgaaaatata tctataatag gctcctaact
      661 aatcaattag tttagtgggc ctttttttac tagatagata aaggttgtac tatagtgtaa
      721 aagacaataa attttatact aaaattactt taaagagaac tagagagaaa ggtacaataa
      781 atgaaaacca taaatcttat aaaagtattt atagcatcac caggtgatat agcagcaaaa
      841 aggaatgaag tagaagaaac catatataga tggaatatag aaaatacaga taatacaaaa
      901 gtgattttga tgcctatacg atgggaaact aatgcgattc ctacttatgt aaataatagc
      961 tctgggcaaa gtgttattaa taaacaaatt gtagaaaata gggttctgct gcaaagtttt
     1021 aaaaataaaa tcagtttttg tattatttct tcattttaag ctggaattcc caataatatc
     1081 ctgatttcag tgcagactga aaagccgaag agagtgcctt cttttcggct tttcttgtag
     1141 attcctcgaa tggtttccat ccctttaatt gtggtggaag ctgtacgtag actttgatac
     1201 aatttatgtc gacgttttat cgatcgatg
//
LOCUS       DAYYHX010000334         1228 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000334 DAYYHX010000000
VERSION     DAYYHX010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.338"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..1061)
                     /locus_tag="ACHA6Y_002908"
     CDS             complement(21..1061)
                     /locus_tag="ACHA6Y_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8988921.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
BASE COUNT          356 a          198 c          176 g          498 t
ORIGIN      
        1 atcaatttgt cagtgaaatt tcactctaac cagtctttcg taatagtttt tgggaagctt
       61 tcagaattca aacgtttttc acttgatact ttagcagcta ttggtttttc gataaaccaa
      121 ttttttacat agacaaaaat caggttctgg atattttcaa ctttttcggt ttttaatttt
      181 tgataggctt ttaacagtgt cgtgtataat tcttgttctg cattgacata ataactatcg
      241 agttcttcaa aaattattga aataccactt tctgtttcgg cttttttctt tgcattatga
      301 atagtctgga cagttttttg agcttcactg taacttgaag aaaatgtttt tattaaattt
      361 aatactcgtt cagggataaa cgtagcaatg ctgtcgtttg tcataattgt ctcaaaattg
      421 tctaacaaca attgatcttg taggcggtct ttttcagtgt ctatattgtg tctattagtg
      481 tctaatatat tattattaag ttctggtatg attttcatac cctctgattt atcaacgttt
      541 tggcacggta tgattttcat accctcttga gccccaaggg attcagcgtc tgatttttcg
      601 tcgagggtgg gtatgatttt cataccctct gatttatcaa cgttttccct attctctcta
      661 tcaaattttt cgagtaagta gatatcattt tctgagactt ttaattctgc tagatatagt
      721 ctattaggat tatttttccc taactgtttg ttaaatccca tctgcttttg taataataaa
      781 ccaaaggatt ccaactcttt tttgattgct atcgcttttt gtttttggca gtttaatacg
      841 ctacatagtt catttgtcgt ataagtaaaa taaacattac cttcttcatc gatttgccgt
      901 ttgctgattg cgatctctaa tcttgctttt aaaagcatat aggcaatctt agttgaatct
      961 ttcatttttt tgtattcgtc agaaacaata aagacttttg gaaattgata aaataattcg
     1021 ttgtaaactc tatccgcctt aaaaaaatta aaatcagtca ttgtagatcc tctactttca
     1081 cgttgtttta tttatgtaaa attacctact cttttcgaaa atagcaaaga taaataaaaa
     1141 atgcaaacgc attaaaactg ttttttaatt aaatactacc taaaatcaga aataaaatga
     1201 attatgtact acctttaaag tcattaag
//
LOCUS       DAYYHX010000335         1220 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000335 DAYYHX010000000
VERSION     DAYYHX010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.339"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1192
                     /locus_tag="ACHA6Y_002909"
     CDS             <1..1192
                     /locus_tag="ACHA6Y_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8988922.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTPDGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDN
                     RSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIV
                     VVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQ
                     YHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDN
                     KTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRK
                     KENE"
BASE COUNT          496 a          184 c          263 g          277 t
ORIGIN      
        1 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
       61 accagacgca tctgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      121 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      181 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      241 tgacgccgat aaatatgagc caacagtaga aggcgaaaaa gtagaagttg gaggcacagt
      301 agatctaaca gataatgtta ctaacttgcc aacattacca gaaggaacaa cagtaacaga
      361 tgtcactcct gatggtacga ttgataccaa cacaccaggt aattacgaag gggttatcga
      421 agtaacgtat ccagatggta cgaaagatac agtgaaagtt ccagtagaag tgacagacaa
      481 tcgttccgat gccgataaat acaatcctga aggacaaaaa gtgactacag acttaaataa
      541 agagccagac gcatctgagg gaattaagaa caaagaagat ctaccaaaag gaactactta
      601 tacttggaaa gagaaagtag atgttagtac agctggaaac aaaaaaggga ttgtagtagt
      661 aacatatcca gatggatcta aagaagaagt agaggttact atttctgtag aagataaaaa
      721 agcaccgaat aaacctcaag ttgatcctat tacagagggt gaccaaattg ttactggtaa
      781 aactgaacca aatgcagagg tgacagttac attaccaaac gggagtcaat accatgggac
      841 agctgataaa aatggtaatt ttacagttaa agttcctaaa ttagaggcag gaacaaaagt
      901 gatagtaact gcaactgatg aatctggtaa tactagtgaa cctactaatg tagttgtttc
      961 ttcaaacgaa aaagacagtg aaaaagccgt tagtaaagat aataagactg acaatcaagg
     1021 tagcaagcaa aacaaaaata gaggcaaaag cagtcctcaa aagcagtcct caaaagctta
     1081 tcctaagacg ggagagattg atagtaatat ctttactatt agtggtggtt taatcctgtt
     1141 aggaacttta gggttattag ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa
     1201 tttcttaaat cttctctgaa
//
LOCUS       DAYYHX010000336         1215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000336 DAYYHX010000000
VERSION     DAYYHX010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.340"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACHA6Y_002910"
     CDS             414..947
                     /locus_tag="ACHA6Y_002910"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8988923.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAYYHX010000337         1205 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000337 DAYYHX010000000
VERSION     DAYYHX010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.341"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..193)
                     /locus_tag="ACHA6Y_002911"
     CDS             complement(<1..193)
                     /locus_tag="ACHA6Y_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988924.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYG"
     gene            339..>1205
                     /locus_tag="ACHA6Y_002912"
     CDS             339..>1205
                     /locus_tag="ACHA6Y_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988925.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAF"
BASE COUNT          372 a          179 c          173 g          481 t
ORIGIN      
        1 ctccataatt gttaaatgtt gtatagttga ttggcccttc aaaaatagaa gataaccctt
       61 tgattcgtga aagatgaaaa gataaatcac ctgttgtata aatttcttga gcatttctca
      121 taaaatttac tgaatataaa gtactaagta agataagaac tactaaaaga aatgcttttt
      181 tacttatttt catagatttg ttgttctcct tttttaattc tattttcagt atactagaat
      241 tgtctttttt taataagttt tgcgttaaat ttttcatagc actagtatga tatactagaa
      301 aagaatcaat ttttattact tttaaatagg agaaaaaaat gaaaaaaaac aaaaaccaaa
      361 taatagatat catttttatg ttatttctaa gttttgctta cgtcatccct ctattcatgt
      421 ctatgggaat ctatcatagt gtcaatcagg atacttactt tcatttatct cgaatcatag
      481 gactagataa tgtatggagt tctccagtaa acttcaataa cttcgatcat catggaacaa
      541 tgatgaatat tttttatcca tggcttactc tttatccagc ttttttattt tataaaatga
      601 tgggaaatct tgttttaagt tataatatct actatttttt tatcactttt ttaacaatgg
      661 tcgtttctta tttttctatg aaacaaataa aaaacaatcg ttatatttcg ctgttatttt
      721 cgattattta tacattttcc gcctatcgtg ccattgacat attccatcgt gcttcattgg
      781 gagaagctgt cgctttaaca tttttgcctt tgatactaat ggggtgttac gaaatttata
      841 ttagagatta ccaaaaatgg tactggttgt ctattggcat gacgctagta gtgtatactc
      901 atttactttc tgtcgctatg gtttctgtat ttattggagg aacgttgttt ctttcttttt
      961 atttttggga ccaaaaaatc gctagactct taagtctttt aaaagctacc gtcctcacct
     1021 tttttttaag tgcgggattc ttaattcctt ttattcaaca atctagagca caagaattga
     1081 aagtcccact gggaaaagaa ttaagtggta tggctcctag tgatatgctg acacacattt
     1141 taaataataa ctataacaac tatactattg gattattctt atttctcgga ttgattggtg
     1201 ctttt
//
LOCUS       DAYYHX010000338         1195 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000338 DAYYHX010000000
VERSION     DAYYHX010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.342"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHA6Y_002913"
     CDS             <1..1076
                     /locus_tag="ACHA6Y_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8988926.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAYYHX010000339         1193 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000339 DAYYHX010000000
VERSION     DAYYHX010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.343"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..424
                     /locus_tag="ACHA6Y_002914"
     CDS             <1..424
                     /locus_tag="ACHA6Y_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988927.1"
                     /translation="LFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDY
                     RGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNH
                     VRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(485..850)
                     /locus_tag="ACHA6Y_002915"
     CDS             complement(485..850)
                     /locus_tag="ACHA6Y_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM8988928.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(926..1171)
                     /locus_tag="ACHA6Y_002916"
     CDS             complement(926..1171)
                     /locus_tag="ACHA6Y_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8988929.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
BASE COUNT          400 a          215 c          231 g          347 t
ORIGIN      
        1 actattttta ctatcaatat taacgctagc aatggcaggt tgttctgact ctggcttagc
       61 aactgaactg atcacaaaat caactgaagc cacgacagag gagttaaaat gtggaaacga
      121 tcacagtgac tacagaggct taaatcgacg atgcatggga atattaggca gaagcaattg
      181 taagcatcga tgtagtaatc gaagaaggtg tagaagaagc aacagaaaaa tcaaaagaaa
      241 gatcaaaaga agcaatctgt gctgtgcatc gtatacagac gtcgaagatg cggtactttt
      301 aaaccacgtc aggagatgtt catttatgtc tagggaagtt ggcgatgaac caaaactaga
      361 gtacgctttt agttttgcac gtgaggatga agataaccct aaaacttacg acattttagc
      421 ttgatctaag atggttaaag ctttttaatg aagtaagagg agatagaagg cttttctagt
      481 tatattaggt aagataaatt cctttttgct ccaaatttgc taaacattct gctagataaa
      541 cttcatcgtg ttccggataa atcggcacgt tgcgctctac agtaggtaaa tcaaaaaatg
      601 cttcgcatga tttgccacga tacataggat ttttccactc tggtgctggg ctgtaaccac
      661 gtttttccat ttcatccatg acaagctgat gatattggaa taatctataa ggagaatagt
      721 gaaaaacata atccacagta gcatgtttct ttccccagcc atttcctctt aatgcacagc
      781 attcccgatg ttgtcctaaa agctgttgac gaggaagctt tgaaatcaat ttttcatgcc
      841 ataatcgcat agaaatgtct cctttatata tagattgtta accttagacg ttaaatgaaa
      901 aaaccagcac caaggctgga aaaaatcact tcagtttgct tgctgtttct gagcgaccaa
      961 ttaggtaatc aacggaaata tcgtaaaagt cagcgatata gattaattca atgatgcttg
     1021 gaatttcttc tccttcttcg tacttacgat atttgaataa aggaatactt aacgcgctag
     1081 aaacagctac ttgagataaa gatcggccag ttcttaagaa attcagacga caacgtaaag
     1141 tatccagtaa aatagcttct tcgatactca tttaatcacc tttcattata gta
//
LOCUS       DAYYHX010000340         1192 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000340 DAYYHX010000000
VERSION     DAYYHX010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.344"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..562)
                     /locus_tag="ACHA6Y_002917"
     CDS             complement(<1..562)
                     /locus_tag="ACHA6Y_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8988930.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQ"
     gene            complement(672..>1192)
                     /locus_tag="ACHA6Y_002918"
     CDS             complement(672..>1192)
                     /locus_tag="ACHA6Y_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM8988931.1"
                     /translation="VIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVYISYNYPRKG
                     IFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVASWALMQL
                     GSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVASLSMIGV
                     LIGRKVLKKYKK"
BASE COUNT          438 a          233 c          162 g          359 t
ORIGIN      
        1 cttgatattt ttgaataaaa tttgtattct ctgtcatttt ctttaacaag aatacaccaa
       61 ttactaacat ggcgattaga accaatccaa ttttaaaatt aatgaaaaag cctaaggcaa
      121 ctgataaaac aggtgttact atggcttgag cactatctgg aatcaagtga gcaattgcca
      181 tatgtgtttt agccgcgttg tcatcaattg tctttcgaat atatcccgaa gaatttaaat
      241 caaaaaaacg aaaacttgct tctgttaaac cgctaattcc cttttttctc aaattagtct
      301 ctaagcgaaa cccaaggata tgagaaaata agccagaaaa aatataaaat aaagctccta
      361 aagttaacca agcaacagtt tgaaccgcat aactcttagc taaatattca tctcctacaa
      421 taattagaga cttaagaaat ttgtaaatac tgtaatatcc atagactgtt aatacgacag
      481 aaaaatcaga aaataaagtg gctaaccatc cgtagtattt cattttaggg acataactaa
      541 ataatttttt gtatactttc attttttcct cccccgcatt caatttcttc tcttattcgt
      601 ttacaactgt taatgctttc cgtaatagcg taaattagag atgtacaaac agatagaagg
      661 taaactacgt tctatttttt gtattttttt aagacttttc ttccaatcaa tacaccaatc
      721 atagataaac ttgctacaac taacactgaa atgattcccc atatacctac cgcatagtgg
      781 atcattgcat ccattgttgc agcagattgt cctttatcta tccaatcttg acgaaaacga
      841 tctgtaaaga accatatagg aatccactgt ccggcagcac tgcctaattg cattagagcc
      901 caagacgcaa cttgaggaat tgctcgatca tatccaaata cagatagaat aatatcagct
      961 aagatcgcca tactaatcga actgataaac caaggtaagt acgccccgcc cattaacgta
     1021 aaaaataaca ataaaacaat tgtgtaaata gtaaaaattc cttttctagg gtagttatag
     1081 ctaatataaa caaatatgat tccagataat aatccaaaaa tccctgggga tatgctgtga
     1141 ccaaacgccc caccaatact actaaccatt attgaaaaaa agtaaattac ta
//
LOCUS       DAYYHX010000341         1188 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000341 DAYYHX010000000
VERSION     DAYYHX010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.345"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..1188
                     /locus_tag="ACHA6Y_002919"
                     /pseudo
     CDS             137..1188
                     /locus_tag="ACHA6Y_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          400 a          201 c          224 g          363 t
ORIGIN      
        1 ttgtaaacac ctctttgttt ctttttactt agtctatcat gtaatcgtcc aaattggttt
       61 ctaagacata aagttttagt ttttctttat cattggttat aattaaatta taaaaagatt
      121 tgaaagaggt gagaaaatga ttggcgaaga taaattattg atgactatcc agctcacaga
      181 cttaaatgaa gaagaaaaaa acaggaaata agcgattggg ctattttgac acgtctgttg
      241 atccagccaa catttatccg ctctttcaca caacaagcgg atccgtatga tcttcgaaag
      301 cttcaagaaa aaataatgct agcatcggta agagacgaaa gttggttagt tgtaggaaat
      361 gacgagaacg aatgtagttt tcgcttggaa gataatcaat tattgataaa aaatgtattg
      421 tctatctctg ttttcaaaga aaagcaattt ttgattcgag acttcattca gaaaaaaatg
      481 attgctcatg gggtgttcgc ctatatgcga gcttatagcg agtttatcta tcacaataca
      541 aaacagatct ctcaacggct attgtttgag aaaaaggatg aaatcgaaca tttacctaaa
      601 atgaaacaac aaaacgggga agtagtagtc gattgcaacc aatttccagg atacgatctt
      661 ttttaccaag gtttatgttt cacttcgtgt tgggaaatgt attatagtcg ctattatcat
      721 caaatcatcc ctaaaccgat ttttctcgac atccaacaag tagaaaaagt gaaggaatta
      781 gaaaacgaag tcatatgtat ccagctttat cgagacccct ttaactggca aaagccaaac
      841 aatcaaatgt ttcaatccta ttttagggac cagcttgggt ttgatcatct tgcttgggac
      901 aatggtgtcg gtttattgaa accgccattc gttgaataca tttacactga ccatacgatc
      961 caatctgtcc agtatcagaa tgcacaaatg caacctgttc ctaaaaagaa tgcttcattt
     1021 tttgtaacaa aaagttatga tatccaacaa ggagattata aagaaaggcg tgttcgcgga
     1081 acactaaatg ctcaggcgta ttttccttgg gttgacgaga atcgatcacg catgatgtgc
     1141 tataaagtta tcgatccgac tgttgctttg gataatggaa tcgaagct
//
LOCUS       DAYYHX010000342         1185 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000342 DAYYHX010000000
VERSION     DAYYHX010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.346"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..940)
                     /locus_tag="ACHA6Y_002920"
     CDS             complement(<1..940)
                     /locus_tag="ACHA6Y_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8988932.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKD"
BASE COUNT          348 a          215 c          213 g          409 t
ORIGIN      
        1 catctttacc tacgtaactc aaaaatactt ccaatggtgt gcgatagtgt aatgattttc
       61 taggaatatg atttcgtttt gatgccaccg attggataaa ggtttcggat accttattaa
      121 aatccatagc tttatataag ccatctttac gtaataagcc attcgaatgt tcatttagtc
      181 ctcgttgaga aggtgttcct ggatcagcga aataaatcga aatatcattg gtattactaa
      241 tctttttcca attcgagaac tcttttccac aatcaaatgt gatggattta aacaagtgtt
      301 tcggtacaga ttgaaaccat tgattcagtc tattctcaat atctatagct tgcctaccag
      361 cgggtttcaa ggtgatgatc actttagata atcgctcaac taacgtgatc accgcacttt
      421 tatgttttcc accgacaatg gtgtcacctt ctaggtgacc aaactcatga ttgaactgta
      481 tataatcagt tgttctatcc tgaatcgaac ggcgaaaagc ttgttttcct cttttttctt
      541 tatgtccgtt tggttttctt ttccctttca ttggtaaatc gtgagggtca aaggttttat
      601 ctttaaacat ccgatatagt gtacgtaccg aacaagcaat aggaaactct gctcgaccaa
      661 taaggacatc tggagtccac ccttgagcca cctttttttg aatgtattct tgttgcccat
      721 ctggtaacac aatcggacgt cttccgcagt tacgtttgtt tttcttatat tgttcatagt
      781 agcttattgc gcttccacca gatttaagaa aacgatagac attataaatg gtttgtcgag
      841 aacgttgtac cagatgggct acttttgaaa cagagtgtga ttgattgaaa taagcttcta
      901 tcattacgag ttcagttggt gtaagatggg tataggtcat ttgtactcac tccttataat
      961 tctttcgacg gaactatttt gagtgtagca caaatgactt ttttagttgt ctagcttaat
     1021 tttacaatcg gcgaaggtaa aaaaataaaa aaatgtgaca agtgttttga atcagctgtg
     1081 gtaaagtttc ctcaatttaa actaaatcca gctgcctcga tacaatttca aggacatgta
     1141 tttgatgttt tagctaaaga taacataaaa aaatacaaaa aaggt
//
LOCUS       DAYYHX010000343         1185 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000343 DAYYHX010000000
VERSION     DAYYHX010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.347"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1169
                     /locus_tag="ACHA6Y_002921"
     CDS             <1..1169
                     /locus_tag="ACHA6Y_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8988933.1"
                     /translation="YKPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQ
                     RFKCKSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYR
                     VMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPY
                     LERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNH
                     RVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIA
                     DRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFL
                     PQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          363 a          239 c          237 g          346 t
ORIGIN      
        1 tgtacaagcc agaagagtgt tcccattgtc tttgtgtcgt cccctctagg atcattcgtt
       61 gggggacgac aaccgttcgg ttgttactga atgatgtgtc tgagtatcgc acttatctgg
      121 agctgaagaa acaacgattt aaatgtaagt cttgccaaag aacattcgta gcggatacct
      181 ctgttgctga aaaacactgt ttcattagtc aaaaagttcg ttggtctgtc attgctcgct
      241 taaaagagaa tacttccatg acggaaatcg ctcgtcagaa aaatatttcc acttcttcgg
      301 tctatcgtgt catgaagcga ttctaccgcc cattgaatcc tttcaaacag actttgccaa
      361 aggtcttatg ctttgatgaa ttcaaatcgg ttcgaagtgt tgccagtgca atgagtttta
      421 tcatgatgga cggacaaagc catgcattat tggatattgt cgaaaatcga cgcttgcctt
      481 atttagaacg ctatttttct cggttttcat tagcgactcg tgaagccgtt caattgattg
      541 ttattgatat gtatgcgcct tatgtttcgt tggtgaaaaa gcttttcccg aatgctcagt
      601 tgatcatcga tcgtttccac atcgtccaac atattggcag aactttcttg aatcatcgag
      661 tgaaagaaac cacggtacga cttaaaggaa attcccactc tcaacgaggg ttagggaaaa
      721 aattgaagcg ctattggaaa ctcctacaaa aagaagaagc aaagttaaac tatgaaaaac
      781 gggtttggcg tgccagtttt aaagattatt tgactgaaag tgagattgcc gatcgactgt
      841 tagctgcttg tcccaactta cgccaaggct atcagttata ccaagacatt ctttatgcgg
      901 taaagaaaag agatcagtca ctatttgagg attgtttaac tagagacatt agcggtctgc
      961 cagaaaccta tacaaccact ttacgaacgt ttaaaaagtt tctgccacag atccaaaatg
     1021 cgcttcacta cagctattca aacggaccat tggaatgctt aaacaaccac atcaaagtat
     1081 taaaacgcaa tgcgtacggc tttcgcaact tttacaattt caaactacga attatgattc
     1141 gtcatggcaa aacattttta accaaataaa cagaacagaa agagg
//
LOCUS       DAYYHX010000344         1179 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000344 DAYYHX010000000
VERSION     DAYYHX010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.348"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..700
                     /locus_tag="ACHA6Y_002922"
     CDS             <1..700
                     /locus_tag="ACHA6Y_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8988934.1"
                     /translation="FKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNS
                     AENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIK
                     GIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPS
                     VQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQM
                     KRDAGAVGGTLYAT"
     gene            690..>1179
                     /locus_tag="ACHA6Y_002923"
     CDS             690..>1179
                     /locus_tag="ACHA6Y_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8988935.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIK"
BASE COUNT          454 a          147 c          216 g          362 t
ORIGIN      
        1 tttcaaagat acttggaaaa atattaaaga tacggcatac aatacgtgga tttctataaa
       61 aaataccatg attaatacat ggaataacat taaggactct ttctggaata ttgttactgg
      121 aattgttaat tctgctgaaa atgcatggac aaatctaaaa aatggtgttt caaatgctat
      181 taatcgggta aaagaaatct ttaattcgtt aagggaagtc aatttatttg aaattggcaa
      241 gaacatcatt gatggactta taaatggtgt aaaagaaaag tggaatgctt tgaaagatac
      301 tatcaaaggt attgcaggta atatcaaaaa ttggattaaa ggagctttag atattcattc
      361 tccatcaaga tggatgcgtg atatggttgg taaaaatatt gttcaaggta ttatcgttgg
      421 tattgataaa gagcaaagta agttagatca aaccatgaca aatttagtaa aaacaccgtc
      481 tgttcaacct gtagtaaatg gaataagtca cctaccaatt acacaaacaa agcaaaatat
      541 atctgctagt gaaacaaaag aaattcattt acatttaaat gtttatggag atttacctga
      601 ttcaatggtt cggaagatta ccacaaaaat taaaacagaa ttgacaagac aaatgaaacg
      661 agatgctggt gcagtaggag gaacattata tgcaacttaa gagaggacaa ttttttatta
      721 atcaacatta ttcttctgaa tttaatgtgt atattcaaaa caggcctgct tctgtttcag
      781 ctagtcgagt aattgaattg agagagcgag aaggaaatga ttctatcatt attgataaag
      841 gctattacaa aaatgtgact agaaagattg agtgttacta taaagcacct tcaattgata
      901 tggtgcagga gtgggaagat cgaatcactg aatggttaga tatgtcgtct tatagtgatt
      961 ttattcttta ctatgatgaa caatatattt atcaagctgt agtaatagaa ggacctgaat
     1021 ttaaaggaac aagaaaaaca ggaaatattg tcccttttga atttacagtg agtattcgac
     1081 catttaagga aaattacaat ggtagattta ctattcgaca aacagaaact tttgaaattt
     1141 ataacccaga gaagtattct tcaaaaccgc ttattaaat
//
LOCUS       DAYYHX010000345         1172 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000345 DAYYHX010000000
VERSION     DAYYHX010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.349"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..289
                     /locus_tag="ACHA6Y_002924"
     CDS             29..289
                     /locus_tag="ACHA6Y_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988936.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(651..890)
                     /locus_tag="ACHA6Y_002925"
     CDS             complement(651..890)
                     /locus_tag="ACHA6Y_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988937.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          415 a          164 c          219 g          374 t
ORIGIN      
        1 atggaaaaac attaaggatg gatgaaatat gaataacgaa actatcattt taggaaaaaa
       61 atatatttgc caacctattg gcttgaaaca aaaagtggtt ggagaagtaa tcagtaaatt
      121 agaaaattgt gttgtgatct gcgtagacga atatcatgac gtcgatcgca atgaaatatt
      181 agaaaaatgc ggaaaagtag tggcaaaata tactcacgtt tatgagttgg aaacaacaga
      241 atatctatat aactatagca acaaatatga acatacttta gtttcataaa gatttaaata
      301 aagtgccttc aaaagcgaga agaccaagct tttgaaggca ctttatttag ttggcagtga
      361 ttgttcatga tttttgattt attcaaaagg ttcgcctaga gaatctgtct ctgtaacgag
      421 gcctggtgga gaaactcgat aaaaacctat actgccagaa ctgtcctatt ttcggataga
      481 caaactagta gttttaaaaa gatcatccga tccaatcatt tcaaaaaatg caaaactgat
      541 atcaggatca tttagctcag gcttgttatc gtaaagcatt tgtaaaggtt gcatttagtt
      601 aggagtaaac gcgaaactat catcattatt tgctgaagaa gatgaagaag ctaattcaga
      661 gtttgtttga aataaaggag acgtgctgac agtttcttta ccctgttttt tgcagaggta
      721 gaaactttta tatcggaagt ttctaccgga gaatttttct catttgtaga aattgttgaa
      781 tcgatactat tcgaaatttg cgtatctgaa ggtatgttct ttgatgcaga agtcccacaa
      841 gcagtaagta acaataaatt tgataaactg ataaacagaa ttcttttcat tatgtacaca
      901 ctccttatag tatcgtttag catacaccta tgctcagtat taggcaaaat caatgcaaaa
      961 ctatcaagtc ttttatagta tctattagtc ttagtaatac atatatggag agggtgatat
     1021 cccagttcaa accagataca ttggttacca tctgtattaa cgattatgcc tctaaacgct
     1081 ttttttatga aaaaatttat aagtgaaaac gtaggtatga tattatctag aggtaatttt
     1141 tttgggtatt taaatagttg ctaggaggat ag
//
LOCUS       DAYYHX010000346         1171 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000346 DAYYHX010000000
VERSION     DAYYHX010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.350"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..783)
                     /locus_tag="ACHA6Y_002926"
     CDS             complement(7..783)
                     /locus_tag="ACHA6Y_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8988938.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            906..>1171
                     /locus_tag="ACHA6Y_002927"
     CDS             906..>1171
                     /locus_tag="ACHA6Y_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFM8988939.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHE"
BASE COUNT          387 a          200 c          167 g          417 t
ORIGIN      
        1 atttttttaa ataaagatgt tgtctccatc ttcaggcact aaaacttttt cggaaattcc
       61 tttttcttta ttgaaagact ttaattcttc tcttgacagt ccccaatggt ttacagcttc
      121 catatgaacg gcaataattg tggaatctgg tattgcttta tgcaattcat ataaatcgtc
      181 tttccccata ataagagatt ctccaactaa aaaatgatta tctccagcat ttgctactat
      241 gatttctgga ttatactgat caatagtttg tttaacagct tcataccaaa ctgtatctcc
      301 tgtaaagtat agtgtcttct cagtcgaatg tttgaaaaca ataccgcaaa cattacctac
      361 ataattcaag atttctcctc tgccatgttt gccttttgtt ttaaccaatt gtatagattc
      421 aaaaactgtg tcttctcgta gtacctccac atttttaaaa ccatatagtc gaatttcatt
      481 cgcatcattt tcatcttgca caaataattt tatttcttta ggtaggacct ctttcgctat
      541 atcgtcccaa tgatctagat gtaaatgtgt tacaataaca gcatcaatac cgttgataat
      601 atcttcgact ggcattggca aatcaactag aggattcatt tgatcttttc ttggtgatgg
      661 aaaaggacct ttcgaaataa atggtggaag tgttcctttt tccgctaaca ttggatcaat
      721 taaaaatttt ttacctccat agtctaagat agctgttgca ttacgaatgt gatgtatatt
      781 cataaacgtt gtctcctttt tgttgatatg aaatattttt agtataattc aattccatac
      841 aataaataaa ccgaacaaaa aaagtctttt actattttta cttaatttat gaaaggttgt
      901 ttaccatgtt agaccaaaca gatataaata tcattgagga actatcaaaa aatagtcgaa
      961 tcacaatgaa agaacttgga gaaaaagtac atttgactgg acccgctgtc tcggcgagag
     1021 tgacaaaact agaggaaagt ggagttattg aaaattatac agtaaaatta aatatggaaa
     1081 agctagggtt ttcgattcac gcttttttaa ttataattac gcaaaattta aatcataaac
     1141 cctatttatc atttattaaa aatcatgaaa a
//
LOCUS       DAYYHX010000347         1167 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000347 DAYYHX010000000
VERSION     DAYYHX010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.351"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACHA6Y_002928"
     CDS             <1..963
                     /locus_tag="ACHA6Y_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8988940.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          393 a          219 c          233 g          322 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagt
//
LOCUS       DAYYHX010000348         1161 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000348 DAYYHX010000000
VERSION     DAYYHX010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.352"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACHA6Y_002929"
     CDS             <1..969
                     /locus_tag="ACHA6Y_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8988941.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          394 a          204 c          232 g          331 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt a
//
LOCUS       DAYYHX010000349         1160 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000349 DAYYHX010000000
VERSION     DAYYHX010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.353"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1141
                     /locus_tag="ACHA6Y_002930"
     CDS             14..1141
                     /locus_tag="ACHA6Y_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8988942.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          419 a          200 c          224 g          317 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa
//
LOCUS       DAYYHX010000350         1154 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000350 DAYYHX010000000
VERSION     DAYYHX010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.354"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..317
                     /locus_tag="ACHA6Y_002931"
     CDS             <1..317
                     /locus_tag="ACHA6Y_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8988943.1"
                     /translation="IKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRHNLRHIRFHD
                     IRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATTAFEKLLKKD
                     IK"
     gene            1018..>1154
                     /locus_tag="ACHA6Y_002932"
     CDS             1018..>1154
                     /locus_tag="ACHA6Y_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8988944.1"
                     /translation="MTIELEEHLLWFKYDPSEKFVKDLYKVWDTEVVFLAIETSLLVN
                     L"
BASE COUNT          387 a          198 c          180 g          389 t
ORIGIN      
        1 atataaagaa attaagacac tattttatat ttgatacaaa acaagacgga actttaccac
       61 gtggaagtta tctgtataag aagttcaaac gattcaccaa acgtcacaat ttgcgtcaca
      121 ttcgttttca cgacattaga catacatcag ctacctattt gcttagcgat cctaatatga
      181 ctccaaaaga gcttcaaaaa cgattaggac accgagattt taatactaca atgaatgttt
      241 atggccatgt cttacgcaaa gaaaaagata cagcaactac agcgtttgaa aaactattaa
      301 aaaaagatat aaaataacac taaaaaatta tattttcggg tataaactcg ggtaggatat
      361 ataaaaactt gggataattt gcggtatata agcatttaaa aatgctgtaa attcgcttat
      421 cttcatttaa aataccatta aataacgaac tcttaatcag cgggtcgcgg gttcgagccc
      481 ctcacggccc attgggtgcc aaacccacgt catcagtaca tctttggcta cttcggtagt
      541 ttcgaaaaag atgtacgtga tgacattttt tatatttcgg gtatgaaatt cgggtatgta
      601 ttttagtttt ttaattttta ataacaataa aaaagcactc aaattgagtg ctttttttaa
      661 gttcactatc actaaaacta tcactagttt cattttaaag caaacatgtt tctatcactg
      721 attttttaaa tctagcgaca aatccttatt ttacaggcgt tcgttcaatt ctttagcaag
      781 gtcttcaaat cctggtttgc ctagaagtgc gaacatgttt ttcttatgag aaccaccctt
      841 ttcatttgtt ctaaagatat ataaatgttg atacatcaat gtttattctt atttttctat
      901 ttacataaat ttcgtcgttt ttgataaatg ggtactagtt tgggtaccgg ttgctaccct
      961 atttttatcc tttacaaaac aaacattagt tcgtatactt tcgttgaggt gaaaaacatg
     1021 acaatcgagc tagaggaaca tctgctttgg tttaaatacg atccatcaga aaaatttgtc
     1081 aaagatttat ataaagtctg ggatactgaa gtagtctttt tagcaattga aactagcctg
     1141 ctagtcaatc tcca
//
LOCUS       DAYYHX010000351         1117 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000351 DAYYHX010000000
VERSION     DAYYHX010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.356"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..84)
                     /locus_tag="ACHA6Y_002933"
     tRNA            complement(1..84)
                     /locus_tag="ACHA6Y_002933"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48..50),aa:Leu,seq:caa)
     gene            complement(105..175)
                     /locus_tag="ACHA6Y_002934"
     tRNA            complement(105..175)
                     /locus_tag="ACHA6Y_002934"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(141..143),aa:Cys,seq:gca)
     gene            complement(221..292)
                     /locus_tag="ACHA6Y_002935"
     tRNA            complement(221..292)
                     /locus_tag="ACHA6Y_002935"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(258..260),aa:Gln,seq:ttg)
     gene            complement(304..376)
                     /locus_tag="ACHA6Y_002936"
     tRNA            complement(304..376)
                     /locus_tag="ACHA6Y_002936"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(341..343),aa:His,seq:gtg)
     gene            complement(379..452)
                     /locus_tag="ACHA6Y_002937"
     tRNA            complement(379..452)
                     /locus_tag="ACHA6Y_002937"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(418..420),aa:Trp,seq:cca)
     gene            complement(459..539)
                     /locus_tag="ACHA6Y_002938"
     tRNA            complement(459..539)
                     /locus_tag="ACHA6Y_002938"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(503..505),aa:Tyr,seq:gta)
     gene            complement(541..616)
                     /locus_tag="ACHA6Y_002939"
     tRNA            complement(541..616)
                     /locus_tag="ACHA6Y_002939"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(581..583),aa:Phe,seq:gaa)
     gene            complement(652..724)
                     /locus_tag="ACHA6Y_002940"
     tRNA            complement(652..724)
                     /locus_tag="ACHA6Y_002940"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(689..691),aa:Asp,seq:gtc)
     gene            complement(734..806)
                     /locus_tag="ACHA6Y_002941"
     tRNA            complement(734..806)
                     /locus_tag="ACHA6Y_002941"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(771..773),aa:Val,seq:tac)
     gene            complement(822..893)
                     /locus_tag="ACHA6Y_002942"
     tRNA            complement(822..893)
                     /locus_tag="ACHA6Y_002942"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(858..860),aa:Glu,seq:ttc)
     gene            complement(908..997)
                     /locus_tag="ACHA6Y_002943"
     tRNA            complement(908..997)
                     /locus_tag="ACHA6Y_002943"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(961..963),aa:Ser,seq:gga)
     gene            complement(1037..1110)
                     /locus_tag="ACHA6Y_002944"
     tRNA            complement(1037..1110)
                     /locus_tag="ACHA6Y_002944"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1074..1076),aa:Asn,seq:gtt)
BASE COUNT          309 a          300 c          259 g          249 t
ORIGIN      
        1 accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg agtccagcgc
       61 gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg taaccggatt
      121 tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata ccgccattat
      181 actattatca atctataaaa taaagaataa attatagaaa ctggggtagc tggattcgaa
      241 ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc caagaataaa
      301 aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg tgcgttaacc
      361 acttcgccac attcgccatg gcaggggcag caggaattga acccacacta acggttttgg
      421 agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg gattcgaacc
      481 actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc tactcctcca
      541 tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt tgctctacca
      601 actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg acggttccga
      661 cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc tacaccacgg
      721 aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga
      781 tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta cgggattcga
      841 acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg gccaggtaaa
      901 tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat ctacatgatt
      961 tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag ctcaatactc
     1021 tttctcaaaa tgagaactcc ggcagtagga ctcgaaccta cgacatcatg attaacagtc
     1081 atgcgctact accaactgag ctatgccgga ataacat
//
LOCUS       DAYYHX010000352         1091 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000352 DAYYHX010000000
VERSION     DAYYHX010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.357"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(266..970)
                     /locus_tag="ACHA6Y_002945"
     CDS             complement(266..970)
                     /locus_tag="ACHA6Y_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988945.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          310 a          193 c          197 g          391 t
ORIGIN      
        1 ttttttctct atcagaaaat atgtttatta ttagaatttt ttatagttaa ggttaggcat
       61 cttaagaaat caggcaactc caagatatct tcaggtagaa gagaagtcgc ctaacattgt
      121 aggatcatag gtgatttgtt ggacaggtca agctgatatt tgagcctgat gttagaagga
      181 atttcttttt acttgctgta gaaattatta tatgactaaa aaggctggga ttttaaatcc
      241 cagcctttta tgacaaatag aatttttatc gatcagtaat cgctgtcgct gcaaattgtt
      301 cgatcaaagg aatcaaagtc aatttgactt tgtatttccg ttcgagtgct ttttcgataa
      361 cgaactcatt ttcagtagtc atagaaatag ttgctaattt acgaggttca tcagcatttt
      421 tttcattaat aactacttca tgttcatttt gttctgtgta aaatttagga tactcagcaa
      481 cggcttttga aaaatcagca aaacttaaag cggcattcgt atcagttgag cgtgtatttt
      541 cagttgtgtc cgcttttggt ttttccgttt tttcttctgg cacactgact gtttcttctg
      601 taactggtgt cgcaacagct tgcgcagttg gttcatcgtt tttcaatcgt tgatcattcc
      661 agtaagacac gatttcaata agaagattgt cacggaatcg ttcgaaaatc ccgttttctc
      721 ctgaacgaat tctctttaat acagtattga agttgtcatc gattggcacg ttgatccgtg
      781 tatcttcatt tgttttccca gctaatccga tcgttaagat ttgacggttt ttgggatcaa
      841 gcatgacttg taacggcaaa ccataacgtc gcaagctctc ttcttctaga gaacggaata
      901 attgtcctct tacaccatcc aatgaaggac ggaattgatt taataatttt tgtttctgtt
      961 ttttcttcat atgttctctc cttcgctcat accctttcga ttataagaaa aaaacagcca
     1021 aatgaaaagg agtaatgcaa aaaaacttgc gtatacgcaa attacaacag aaaaatttgc
     1081 gtattttccc g
//
LOCUS       DAYYHX010000353         1086 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000353 DAYYHX010000000
VERSION     DAYYHX010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.358"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..>1086
                     /locus_tag="ACHA6Y_002946"
     CDS             52..>1086
                     /locus_tag="ACHA6Y_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8988946.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLK"
BASE COUNT          421 a          129 c          191 g          345 t
ORIGIN      
        1 gttagttaaa aatgaagata cttttcatag aagggaaaag aatgtgatta agtgaaaaaa
       61 atagtaatta ttggatcaaa ccattcgggg ctagctgcag ctcgatttct aaacttatgt
      121 gattcaaatt atgaagttat tctgatagat aaaaaatcag aaattagtta cttagaatgt
      181 gggacatcgt tgttcataaa aagagaagtt agcaatttag aagatctttt ttatcaaaat
      241 agaagagaac taaattctaa gataaaaaag ttgtttttgg agacagcagt tgacaaaatt
      301 gatattcaaa ataaaaagat tatttgtgta aaaaaagatg gcacaagatt ttcccaagaa
      361 tatgacaaac ttatactagc tacaggttca aagcaaatta tgttagatgt accggggaat
      421 aatttagcta atattttttc gataaagaat tttccaaatg ctaaagagat ttttaaagct
      481 ttagatgata atactattaa aaaagtagga attattggag caggatatat tggagttgaa
      541 attgctgaag ccataagaaa aagagggaaa gaagtatatc ttttcgatgt tgcagataga
      601 gtgttgtcga cttattacga ccgtagtttt tcagataaag ttgaagaaat actatcaaaa
      661 aatggaatac atctttgtct atcagaaagt cctaaaaaat atttaggaag aaaaaagatt
      721 gaaaaaatag tcactacaag tggtacccaa tactctatgg atatggtcgt acaagctata
      781 ggatttctat ctaatagtcc aattggcaaa aaggaactct tgagagatgt tagtggagca
      841 tatctaacta atgaatacca gcaaacaaat ataaaagata tatatgctat tggggattgt
      901 gctacaactt tttttacgtc cattaataga agtagtgttg atttttctat ttctaatgct
      961 ctaagaacag cttatattgc aactcaacat attgacaatc aagattttac accttctggt
     1021 tcccaattta caaatggctt ttctatattt aattacaatt tttacgcagc aggtttgaat
     1081 ttaaaa
//
LOCUS       DAYYHX010000354         1079 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000354 DAYYHX010000000
VERSION     DAYYHX010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.359"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..902)
                     /locus_tag="ACHA6Y_002947"
     CDS             complement(24..902)
                     /locus_tag="ACHA6Y_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFM8988947.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT          341 a          205 c          171 g          362 t
ORIGIN      
        1 taaactattt cgaagtacta aacttatgaa gtaagaatac ctaagccaga gccaatgtgc
       61 tcgcctgcaa gcaattgctc ttttgcttgt cgctgtaaac tcgtactttc tggataatca
      121 tgtatttggg catctactcg tccaaagatc caagggactc cactatcgtc ctgttcatca
      181 actgataata atcgataatc ggctgcaaaa agacagattt ttgactcttt tttcaaccat
      241 ttagtaagat tgggtgatag taaccaagta tctgtgtaca tcacttggta atggtaatca
      301 ggaaaataaa gttcaaaaaa acgaatggca ctttcgtaac tatctcttat cttattctga
      361 gataaatctg catcggaagg aatatgaaga gaaaggcact gctttccttc taatcttaga
      421 tccgtgtacc ctttcggaaa atatgtcatt tcgtactcca attcaccaat ccgaaagatc
      481 aagccactag tgtaatgcca aatccaaaaa ccacggtcaa agcgatagct tcctgttact
      541 tggaaatgtt catgaataaa tctagtaaaa gcattcattg ttgccaaata gatagttctt
      601 ggaatcttca atggttgata aatttttttc caactttgag aagctgcata tagataaata
      661 gctaatgctg caaaaggttc ctcttcatag actttgtcta ttttctttgc agtcgccttt
      721 gctttattag gatccattag ttcatgacta tattgctcaa acacctcatc aaggatgttt
      781 tttgcttgta caatcatttg ctctataacg actttgggaa gagttaaagt ttctgccaga
      841 atttggattt cattgcttat tttcttcatc aaatttcccc ttttcatatc tttgaacctc
      901 attgtttaaa tgactgatta tccggacacc atatggtata aacatacttt tattgtacac
      961 taactataaa acttaaactt tctatgaaga aagaatatct gtaagacttt caaggaaagc
     1021 attgaccatc tcaggaaagt ttattcagtt accatcataa acaaaatttt ttgatagaa
//
LOCUS       DAYYHX010000355         1062 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000355 DAYYHX010000000
VERSION     DAYYHX010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.360"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..554
                     /locus_tag="ACHA6Y_002948"
     CDS             <1..554
                     /locus_tag="ACHA6Y_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8988948.1"
                     /translation="KTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEEFDR
                     WREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVRHES
                     ISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPIEEG
                     ALIIATVDDPKQNVFTLEKVGVI"
     gene            554..>1062
                     /locus_tag="ACHA6Y_002949"
     CDS             554..>1062
                     /locus_tag="ACHA6Y_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988949.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          378 a          136 c          238 g          310 t
ORIGIN      
        1 caaaaacaga aggagaaatg ggttcttatt ggcaatcaat tgaagattta attgctgata
       61 tgacagattt tattaatgaa aataaaggcg attttacgga ctggatgaat gaaaggaaag
      121 aagaatttga tcgctggaga gaggaacagg agaatagttt ccaagattgg agagaaggac
      181 aagaatcaga ttacttgtca tggtttgaat caatcaagga tattttgaag tctatcgatc
      241 caggtggagt aatgttagcc gaattaatgg atgcacgtgt agatcttcaa ggtgtacgcc
      301 atgaatcgat ttctgaacgt ttcctatccg atctaaatta tttgtatcaa aaaatgaaag
      361 cagcactttt cacgattgaa tacggtgaaa ttgaagtgac tgatattctt caagacgatc
      421 tcttttcaga taatcacgaa gtcgaaaaaa ttgaaacagt agaatttcca attgaagaag
      481 gagctttgat tattgcaact gttgatgatc caaaacagaa tgttttcact cttgagaaag
      541 ttggggtgat ttgatggtta aagtaaagag aatgatggaa actgatgaat ctggtgttaa
      601 acgtcaattt atgccaatta cacatgtctc tgcagtttta ggcttagaaa aaatgatatc
      661 aggacaatca aaagttcttt cggttaatgg taaatatggt gcagtcattt taacgaaagc
      721 tgatttagga ttagagaatg caattactga actaccttat gcaagcgaga caagcgatgg
      781 tattcttact gctgaaatgt ttcaaaagat cgtaaatgga gaaggtggaa catatatttt
      841 gccgattgct actcctgaac aattaggagg tataaaagtt ggggaactct tagaagttac
      901 tgaagaaggt gttttatctg caactaagca aacagacttc aatttctctg aagaattaaa
      961 aggaaaatta gaatctctaa aaatattaaa agcaggtgca aatatatcaa ttgcagaaga
     1021 tggaacaatt agttctacag gtggtagtgg gactggtggt gt
//
LOCUS       DAYYHX010000356         1035 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000356 DAYYHX010000000
VERSION     DAYYHX010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.361"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1035)
                     /locus_tag="ACHA6Y_002950"
     rRNA            complement(<1..>1035)
                     /locus_tag="ACHA6Y_002950"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DAYYHX010000357         1035 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000357 DAYYHX010000000
VERSION     DAYYHX010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.362"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1014
                     /locus_tag="ACHA6Y_002951"
     CDS             <1..1014
                     /locus_tag="ACHA6Y_002951"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8988950.1"
                     /translation="ALSSIFSYVSLAVVILVGVHLLLTGEVTILYVVGYLLAAIKIKD
                     SFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLKSFDIELRDVSFSYDNNTPI
                     LDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDEGCILIDGYDIKRVSPASLF
                     SKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARLANCEDFIEKLPDGYHTLVG
                     ENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAENEKRIQESLNRLIQDKTVL
                     IISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIYKNLIKKTKLSEKFVYEKEA
                     QSR"
BASE COUNT          352 a          154 c          208 g          321 t
ORIGIN      
        1 gccttatcct ctattttctc atatgttagt ttagcagtag tcattctagt aggtgttcac
       61 ttactgttaa cgggtgaagt gactatactc tacgtcgttg gttacttact agccgcaata
      121 aaaataaagg attccttcga ttctatgaaa gaagcagtgc ttgaaatatt ttatttagct
      181 cctaaaatac agcgaattag agctatgaag gaaaccagta tccaagaggg atcagatagt
      241 cctctgaaat cttttgacat tgagttgcga gatgtctctt tctcatatga taacaatacc
      301 ccaattttag atcatatctc ttttacggct aaacaaggag aagtgactgc gttagtaggt
      361 gctagtggtt ccgggaaaac tagtatcttg aaattagtgt ctagactgta tgattatgat
      421 gaaggctgta ttttaattga tgggtatgat attaaacgtg tatctccagc ttctttgttt
      481 agcaaaatcg ctattgtttt tcaagaggta actttgttta atacaagtat tttggaaaat
      541 atacgtattg gaaatagtca agcgtcagat gaggaagtga aaaaagcagc tcgtttagct
      601 aactgcgaag actttattga aaaactacct gacggttatc atacattggt cggagaaaac
      661 ggaagttcac tttctggagg agagcgacaa cgcctatcca tagcacgggc ttttttgaaa
      721 aatgcgccaa ttttaatttt agatgaaatt actgccagtt tagatgcgga aaatgaaaaa
      781 cgtattcagg aaagtttaaa tcgtttaata caagataaga cagtactaat tatatcgcat
      841 cgcttgaagt ctattgaaaa ggtaaataaa attgtagtga tggatcaagg aaaaatagtt
      901 gatcaaggta ctcatagtga gttgtatcga aggtcagaaa tatacaaaaa tctcataaag
      961 aaaactaaac tatctgagaa attcgtctat gaaaaagaag ctcaatcacg atgatgaaat
     1021 aggctttaaa ttaac
//
LOCUS       DAYYHX010000358         1032 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000358 DAYYHX010000000
VERSION     DAYYHX010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.363"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            243..>1032
                     /locus_tag="ACHA6Y_002952"
     CDS             243..>1032
                     /locus_tag="ACHA6Y_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM8988951.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEK"
BASE COUNT          402 a          205 c          181 g          244 t
ORIGIN      
        1 aagaaacgat gttcagatag gaaaagaaaa atatctaatt ttgtattgct ttttttcttc
       61 aagagaaacg ttttaatcgc ttcttgagtt tgcatgaaag aggctccttt ttacttaaat
      121 tgttaaaact tgaaaatatt actgtttttt tcataaattt agacaaaaaa acagtaaaca
      181 tataggtaga atctctacta tcttatttct tgcatacaaa aaaatcaagg agtgagagag
      241 ttatgagata ttcaaccatc ttaatatcaa catttgcagc cttaagctta tcgcttacta
      301 taaatcagac agtccacaca gaggagatag aaacagccaa atggacagaa accaagtggg
      361 ccaacgtcaa tcgaattacc tttcaggatg aattttcatc cttcaattca ggagtcgcaa
      421 aattaagaaa aggacaaaca gcgaccatca aagcaaaaat tgactattca ggagacttag
      481 cacctatact acaatcagct gttacatttg agaatgttga tcccgctttg tcgattggct
      541 atgacgagaa cactttgact atccaacaca agcaagcgat atttaccttc accgttacat
      601 taaatcagga cttgttaaaa ccagcgcttt ttactgtcaa ggtatcagat ggactaccga
      661 acgatcatca tcatttaacg ccgtatagtc agcggcagac aattgagcaa gattcagcta
      721 tcgactcagg aggagatctt gttgaagaac caacagataa accagaaaat gaaaataagc
      781 cagaagtacc gcctacagaa aatccagacg gagaacagaa accggaaata gaacctggag
      841 aagaacccga tacagaaact caaccagagc cagacaatga atcaaaacct gaaataaccc
      901 ctggagaaaa accagatgta gacccagaag aaaaaccaga tgtgactcca gaaccagata
      961 cggattctgg aaaccaaaca gtaccggaaa caaatccaga cacagataat gaaactgaaa
     1021 atccagaaaa ac
//
LOCUS       DAYYHX010000359         1020 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000359 DAYYHX010000000
VERSION     DAYYHX010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.364"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..717
                     /locus_tag="ACHA6Y_002953"
     CDS             1..717
                     /locus_tag="ACHA6Y_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8988952.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
BASE COUNT          367 a          189 c          191 g          273 t
ORIGIN      
        1 ttgaaaaaaa ggattttagt aacaattggt ctagcttttt tgttagctgg ttgtaccaac
       61 gggaatgaat cgaaaaaaga atcaacaaaa gaaacagcga aagcaacagt tgcaactact
      121 acaacaacta gcacagagag caagtcaact gcagcaagta cagaaacagc agatacctct
      181 acttcgtcag agtcttccaa aacgactgaa acaactcaaa catctacttc aagttcttct
      241 gtagaggctt catcttcctc ttcagaaagc agcgaacaag ttgaacaaac actgtggaat
      301 gcagacaaat ccgcacaatt agaggcattt atgaccaaat ggggccaaac aatgggacaa
      361 caatacaaaa gttatacgaa tcaaatgagt gtggacttat atggtcttaa agtccctcaa
      421 gctattttga acggggagtg gaagatggct attggtggag tgcctgtttc tgcagaatgg
      481 tcagaaagtg ggacaggtca agcagactat caaatcacag ccgtctattc cgatgcagag
      541 acagaaccat atctgaaaaa gcacgtctat ctattcgggt ttcagcaaaa tcagccaaaa
      601 gtattggtga cacaacaaaa tcaggggaat cccgataatt atctttactt caacgagaca
      661 gcgaataatg aattgaaaaa tggctttaat caaatcgttt atggacaagc aaattaatga
      721 gataaacaag gggttgtgac atgtctttta tcacaacccc ttgctcagtt tatttgttag
      781 tcttttattt tttggctcta tactttttga tgttggtgaa cttcaccaac atcttttttg
      841 tataaaaaaa tatagaaaag acaccctgaa atcctttaga attaatttgg ctaccaattc
      901 ataggaggat tcaagatgtc tcatactcag cttatcaaag ataccctaaa tattctagac
      961 ctaaatatct atttcgagga aaattgttta actaaagaaa aatacaaagg gcaaatttgc
//
LOCUS       DAYYHX010000360          998 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000360 DAYYHX010000000
VERSION     DAYYHX010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.366"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            367..998
                     /locus_tag="ACHA6Y_002954"
                     /pseudo
     CDS             367..998
                     /locus_tag="ACHA6Y_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          292 t
ORIGIN      
        1 tcaaagttag tagagaaaat gtagttatat atttaaattt ttaccagaaa gaaatgaaca
       61 ccttcaatta tctaaatctc gaaaatggtt agatgtaatc atcaggaaaa acaatcttaa
      121 acgaattact attcatggtc ttagacacac acatgctagc ttgcttttag caagctagtg
      181 caaatattaa agatgtgcaa gagcgtttag gccactcttc tattcaaatc attatggatc
      241 tatatattca tattacagac aaaagaaaag aagaaaccgt agcgcaattc gcaaaatata
      301 ggctctataa tttatgggac tgatgaatca aaattgattc ttcagtccca ttgttatttt
      361 aggtatatga ttcgtacatt gaaagagttg aaaaactatc ttccgaagcc gtcaaaaagg
      421 accctgccaa aagtattgat ggtggatgaa tttcgttcgc acgcttcaat tgtggataaa
      481 atgagcttta tttgtgcgga tggcgagact ggaaaactca ttgatatttt gcctacacga
      541 aaattgtctc gattaacaca ctattttagt cgctgtgcaa atcctgaagc ggtggaatat
      601 ttagtgacag atatgaacgc cgcctacttc caactgacaa aacgtgtctt accaaatgcg
      661 aaagtcgtca ttgattgttt tcatatcatc aaacatttga atcaggcgtt caatgatttt
      721 cgtgtacaag aaatgaaaag attacaccag ttagggcaac gacgggaagc cgagaaagtg
      781 aaaaagaact ggcgctttct tctaaaaaac cgtcaaaaaa tcaatcacta tgattacaaa
      841 acatggcgca gttttcgtgc acctaaatac ccgctactca ctgaagcaat gatgattgat
      901 cgattgctgg cattttcaac gcctttaaag gacgcctata cttttttcca tgagctgacc
      961 gaagccttta gagacaagga atcagactta ttttttac
//
LOCUS       DAYYHX010000361          984 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000361 DAYYHX010000000
VERSION     DAYYHX010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.367"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..591
                     /locus_tag="ACHA6Y_002955"
     CDS             <1..591
                     /locus_tag="ACHA6Y_002955"
                     /inference="COORDINATES: protein motif:HMM:NF015839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HFM8988953.1"
                     /translation="KSEQDRNERTNILMKRLEASTSNFNNKLDVFEEKTKHARLNFET
                     TAKHYIKRLDEDNLKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDH
                     NKVVDKSNTALKVMTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDH
                     ESAWRYLMLILYAVPYVFFAIILWLIALFFRVFNDL"
BASE COUNT          378 a          136 c          188 g          282 t
ORIGIN      
        1 aagagcgagc aagacaggaa cgagagaaca aacatactca tgaaaagact agaggcttcg
       61 acctcgaact ttaataataa gttagatgtt tttgaagaaa agactaagca tgcaagatta
      121 aactttgaaa caactgcaaa gcactatatt aaacgacttg atgaagataa tttgaaatta
      181 gattttcaac aagctattca agatgaatta agcgatacta aagacgaaat acgtgaagtg
      241 acgaaacaag cgagagaaga aacaaaagag tataaagaaa tcttagaaag taaaataaaa
      301 gaccataaca aagtggttga taaatcgaat acagcgctaa aagtgatgac aaaaggcgtt
      361 acaaatatat tttttgtgct aattattttt gtacttgtaa tgcttgtgac aggtccgata
      421 ggtcatttct ttggtattga acatttatat agctttatta atggttttat tgatgaccac
      481 gaaagtgcat ggcgatattt gatgttgata ctttatgcag taccctatgt attcttcgct
      541 atcattctgt ggctcatagc attgtttttt agagtattta atgaccttta ggtagattta
      601 tatataaaaa gggctaaata cgcaaaatac ccccttaaaa tgcaaaataa atgcaaataa
      661 gggggtaact ccttatgaaa aaacaactaa tagtattata acacgttttt atgccgagaa
      721 aacttattgg ttggaatggg ctatgtgtta gctaacttgt tagcgagttg gttggacttg
      781 aattgggatt aatcccaaga aagtaccaac tcaacaacac ataaagccct gtaggttccg
      841 accaataagg aaattggaat aaagcaacga aaggagttga gtaaaatgag tgagaaaacg
      901 aagaaaatca ttcgagtatc attagctttt tcacaagtag tagtgcaatc attgatagtt
      961 tattatactt accagcaata taaa
//
LOCUS       DAYYHX010000362          969 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000362 DAYYHX010000000
VERSION     DAYYHX010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.368"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..643
                     /locus_tag="ACHA6Y_002956"
     CDS             <1..643
                     /locus_tag="ACHA6Y_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HFM8988954.1"
                     /translation="KETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLIS
                     EYKNEIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDE
                     YSKETDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTN
                     RAKIQTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            646..>969
                     /locus_tag="ACHA6Y_002957"
     CDS             646..>969
                     /locus_tag="ACHA6Y_002957"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988955.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTK"
BASE COUNT          403 a          109 c          186 g          271 t
ORIGIN      
        1 aaaagaaaca gaacggttga gaatgatttc cgataaggtg agtatatact ctcaaaaaca
       61 aatttatgaa atttcaaaaa atcaaactag tatactagaa ttactagatg gatataactc
      121 tgatttgatt tcagagtata aaaatgagat tgaaacatgt gtaaacgaaa taagaaaatt
      181 aaattgggat attgtttctg tgaaaaaaga aatacaagat aaggcgaagg tagagcttga
      241 aattgaagat ttaaaaaaac aagtagaaaa gctatctcat aagagttata aagatgttta
      301 tgatgagtat tcaaaagaga cagatattta tagggaactg aaacgtgatg tggaggcact
      361 taaggaaatt cccaaaaatc taactgatag tgttgacaaa attgactttg gcaatggtat
      421 aaaagttaca gacgaaattg atgaacaccg cggagaattg attaagaatt tagtgaccaa
      481 tcgggctaag attcaaacaa taattaatga aatgtcagat gaaattgata gttatagagc
      541 agagctgaat aaatcagatt ggaaagttaa ctataatgag gtttcaaaga ggtatcaaag
      601 aggtatcaaa aacttagtga agagttatct caagatgaat taaaaatgtt acaaaacctt
      661 gattctttat atcaatcttt atcaaaaact gaagagcgat tggaagaaat tattcaacaa
      721 gaaataaagc ttaaagatta ccaagttgag gttgactcag caatgtcaag aatgaataaa
      781 tcatatcaaa atctctattc tgagagaagt agttcctcgc gcgaaatttt ttctgatatt
      841 acaggaatag aggctactat tcaaccaaat agggatttca ataattatgt taaagagctc
      901 agagcttgta ttaataagga ggacactttt gacagtgagt ttggatctct tattgataag
      961 ctgacaaaa
//
LOCUS       DAYYHX010000363          959 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000363 DAYYHX010000000
VERSION     DAYYHX010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.369"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..511)
                     /locus_tag="ACHA6Y_002958"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="ACHA6Y_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="ACHA6Y_002959"
     CDS             complement(570..>959)
                     /locus_tag="ACHA6Y_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8988956.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DAYYHX010000364          956 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000364 DAYYHX010000000
VERSION     DAYYHX010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.370"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..451
                     /locus_tag="ACHA6Y_002960"
     CDS             <1..451
                     /locus_tag="ACHA6Y_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988957.1"
                     /translation="LALCLSLSAVGCQKSNENNKIKEGQSISSEEEGMSINANGKNET
                     FKPSDYTLEAKKEYVYEYLGLKFKLSDKFRNYIADKKIAMLDDQSPIDKELKYAILTF
                     EKMTEEQKNAVIEKMGDGYKNWQNELERIGTIGIFEKNTSEEKNLKL"
     gene            607..>956
                     /locus_tag="ACHA6Y_002961"
     CDS             607..>956
                     /locus_tag="ACHA6Y_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM8988958.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          405 a          103 c          190 g          258 t
ORIGIN      
        1 acttgcatta tgtttgagct taagcgcagt tgggtgtcaa aaaagtaatg aaaacaataa
       61 aataaaagaa ggacaatcca taagttctga agaggaaggc atgtcaatta atgcaaatgg
      121 caaaaatgaa acttttaagc catcagatta tacattggag gcaaagaaag aatatgttta
      181 tgaatattta gggcttaaat ttaaactctc agacaaattt agaaattata tagctgataa
      241 aaagatagcg atgttagatg atcaaagtcc tatagataaa gaactaaagt atgctatttt
      301 gacatttgag aaaatgacag aagaacaaaa gaatgcagtt atagaaaaga tgggcgatgg
      361 atataaaaat tggcaaaatg agcttgaaag aattgggact attggtatat ttgaaaaaaa
      421 tacatcagaa gaaaaaaatc taaaattata aaatgtgata ctcatactaa aataggagtt
      481 tcaagtgatg gaaaatacga ttgctattta agcacaaata gcggagctga aagtaatctt
      541 ctggatgaat taaaaagaac tgagattcaa attatagata aaaaagaacg tcctaaaaat
      601 ggttttgtgc tatcagaaaa aactgattta gaaaatacag aagcatttaa caaagaatcg
      661 gttaaagatt tacgtaaact accaacaaag gacataaatg gcaaggattt tacaagtaaa
      721 gattttgaga agtatgatct tactatggta aatgtatttg caacttggtg tacagcttgt
      781 gtaaaggaaa ttcctgattt ggttgaggtt cagaatgaaa tgaaaagcaa gggtgtaaat
      841 atcgttggag ttgttacaga tcctgttgat gataacggtg aaaacaagga agcaattgaa
      901 aagtcaaagt taatacatga aaagacaaag gcttcatatc ctttcttaat gccaga
//
LOCUS       DAYYHX010000365          943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000365 DAYYHX010000000
VERSION     DAYYHX010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.371"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACHA6Y_002962"
     rRNA            <1..>943
                     /locus_tag="ACHA6Y_002962"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAYYHX010000366          925 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000366 DAYYHX010000000
VERSION     DAYYHX010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.372"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..582)
                     /locus_tag="ACHA6Y_002963"
     CDS             complement(<1..582)
                     /locus_tag="ACHA6Y_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988959.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYK"
     gene            complement(822..>925)
                     /locus_tag="ACHA6Y_002964"
     CDS             complement(822..>925)
                     /locus_tag="ACHA6Y_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFM8988960.1"
                     /translation="WTCPICHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          286 a          162 c          122 g          355 t
ORIGIN      
        1 tttgtaaaaa tattcgattg aatattctat ttggcacgac tcacttggca gtatatgtat
       61 gtcaatattt ttcatctgtt tcataggtat ttcaaaatct aaactattaa aaacatataa
      121 ttccctgtaa ttatttacat ctaactctac taaatttatc tttggaacaa tagatcctcc
      181 acccattcca ccataataaa aaaaatcttg ttctctatca aaatagcaca tattaagctc
      241 agtttgttta tcatcctcaa taattttata aatcaaacct atataatcat gaatgctggc
      301 aaaaattttt tttaagtcaa gttctaaatc ttcttcgaaa acacatttaa tttctcctac
      361 tgataattga tactctatat tacatgactt aattagtttt ttagatcccc cctccagata
      421 ggcatatgtt tcaataatta tgttcttttt attgcttggc aaatttccgt tatttattgc
      481 ataaattaaa aatgactgtc tatttagccc ataatctaat acaacttctt catactcata
      541 tttctttaaa tatttaatat gaaaatatgc tgaattaatc aaaactcgag tgttactgtt
      601 gttatttatt gttgttctat tgtgtataaa attgtatagg ttgtcatatt ttcttggttt
      661 agtgaactac tcaccactta gctaaaccta acggtttctt acgtttgaag tgggagcttc
      721 ttgggaagag agcatacttg atagcgtatt actttactaa cagaggtgcc tcttatttac
      781 caagctatcc ccgtagttcc tacggttctt gtttatttga tctaagccaa tcccatagag
      841 tgtagcctta gaccttcggt caggatattg atactagcgt tgatatctcg gtcatgatga
      901 gtatgacaaa tagggcaagt ccatt
//
LOCUS       DAYYHX010000367          923 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000367 DAYYHX010000000
VERSION     DAYYHX010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.373"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..747
                     /locus_tag="ACHA6Y_002965"
     CDS             55..747
                     /locus_tag="ACHA6Y_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8988961.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          407 a          143 c          154 g          219 t
ORIGIN      
        1 tttgagatca cttactttta gcatgtaaaa gtattggatt aaggagctat gcaaatgaaa
       61 aagaaattta ttactggtgg gattgtcgtt gcatttctat gtctaattgg ctacacaggc
      121 acgcggtacg caaatgaaat tattttgtgg ggtggagaga cagatattga tacaatcaat
      181 gagaatctga cacagcttga tcatacattg acagataaag agcaagcgat tacagatctt
      241 accgctcgac tagcacaaaa agaacagaca ttgcaagatt caaaggcaga tgtccaagaa
      301 taccaaacaa aagtaaatga attagaaaac caaaaaaatc aatcggcagc agaaaaacaa
      361 aacttagaaa gtcaattagt cagcaaggat gcacaactac aaagcaaaca aaatgaaatc
      421 aatgtcaaac tagatgagat caaccaaaaa aatgatgaaa tcaatcatct gaatgcagat
      481 tggacagcaa gactacaaga agcacaagca aaactttcag aattacaaaa tactgtaaat
      541 ggtttgaatc aacaaatcgc aacactgaca caagaaaaaa caaatgtaga aactcagtta
      601 acagatacac aaaagaaatt agacgaagca actgctgaaa actcaagctt gaaacaatat
      661 attcaaaagt tagaaaaagc aaaagatgaa gtggaagata cagctgataa atctcagcaa
      721 attgttgaag aacatttgcg taaataatct ttcttaacaa gtaaacctaa attagtaaag
      781 aagcaacagt aatctatact aagtagttca gttttggagt acaaaatgac aaaatgtact
      841 tcaaaatgga ctactttttt cattttaact tacataccaa attacataca aaatgattta
      901 taaaatgcaa ttgaaaatga taa
//
LOCUS       DAYYHX010000368          921 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000368 DAYYHX010000000
VERSION     DAYYHX010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.374"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..900
                     /gene="thiD"
                     /locus_tag="ACHA6Y_002966"
     CDS             85..900
                     /gene="thiD"
                     /locus_tag="ACHA6Y_002966"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM8988962.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          332 a          148 c          210 g          231 t
ORIGIN      
        1 ctgagggata ctttgaaaaa catatgatag aaacagtaac agtattatga aaaatctttt
       61 tataaaaatg gaggaatcga tttgatggaa gatcagtatg tacaagcaat gacgatcgca
      121 ggatcagata gcgatggaag cgcagggatg caagcagata tgcatacatt ctttacaaga
      181 aatgtttacg gtgtttctgt catgaccgca tgtgttgcag gtaattctta cggaatcgga
      241 gcaagtgtga cattgccaac agattttatc gacaaagaat ttgaactgat tgcgaaggac
      301 tttcaaatac gtgcagctaa aacaggtatg ctagcagatt caaagttgat tgagacagtt
      361 gtaaaaaatt accaaaaata tgattttgga ccattagtcg tggatcccgt aatcgtaacg
      421 aaacatggaa atcttttact agaagaatct gctttgcaaa gcttgaaaga aaagcttgtc
      481 cctttggccg aagtactgac acctaatttt tatgaagcag aaaagctgac tggacggaca
      541 ttagcagctg aagaagattt tgtgcaagca gcaaaagaac tgcaagatat gggtgcaaaa
      601 aacgttatga tcaaaggaca tcatgctaaa aatgcgcagc aagaaacagt caatgactac
      661 gtattgttcg aatcaggaaa atcattttgg ttaaatgcac cattttatcc taccgaccgg
      721 atcaacggaa ctggcgattc gctatctgca tgtattgcag ctgagttagc gaaaggaaaa
      781 acagttgaac aagcgatccg attggcaaaa gactatgtaa accgtgcaat tcgtgaagaa
      841 ataaatgtag ggcacaaata tggccccatc aatcattggg cagctggtga attagagtaa
      901 aagaaagaag ttgtgaaaaa g
//
LOCUS       DAYYHX010000369          915 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000369 DAYYHX010000000
VERSION     DAYYHX010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.375"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(232..462)
                     /locus_tag="ACHA6Y_002967"
     CDS             complement(232..462)
                     /locus_tag="ACHA6Y_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM8988963.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(692..>915)
                     /locus_tag="ACHA6Y_002968"
     CDS             complement(692..>915)
                     /locus_tag="ACHA6Y_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8988964.1"
                     /translation="AITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLL
                     VHGYEQNNNIDSVELREKVLRSIIDKSIE"
BASE COUNT          334 a          140 c          139 g          302 t
ORIGIN      
        1 ttaatttgac aattaagagt atatttactt cagatggtaa ggcaacaaaa taaaatacaa
       61 taggcagatg aactaataaa taaaatctgt attaaataca gataaattaa tataaaaatc
      121 aaaaggatca taaactttta acaagtgatg aaatgggtgg aaattgattc ttatagacag
      181 tttgtctcat aacattgaag tattttgcaa ttttagcatt gaaacacttt tttaatcttc
      241 aacgttgttc ttataaacaa gatgattggg aatcttttga attaacactg tatagcttag
      301 aacacccttt atagaaaaca ttcgcttagc aatcttaata attccttgtg cccgatgtcg
      361 tttagattca gtaggttcct gctcatcgat catggaaaaa atttgaagga tcagatcctt
      421 aataaattta tttaagagag gatgatccat tgcttcatcc atactcgata aactagtgat
      481 aatgagctac acgtttttct ttcaagttga tcaatagctt ccacgataaa aatatcttta
      541 gataaaatca agaaccgtag gaactacagg gatatttggt caataagagc caccgctatt
      601 agtaaaaaat aagctatcaa gtatgctctc atcccaagaa ttgcctactt caatcgttaa
      661 aaaccgttag gtttagctaa gtgggggtag ttcactcaat acttttatca ataatagagc
      721 gcaaaacttt ttctcttaat tcaactgaat caatattatt gttttgttca taaccatgaa
      781 ctaatagatc acaaatataa agttgtgata tttttccagc taaagatcca ccattaagaa
      841 attcttcaat ggctgtttgt aaaactaagt ctgcagactt tcctataggt gaatgaatat
      901 agttagttat agcaa
//
LOCUS       DAYYHX010000370          907 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000370 DAYYHX010000000
VERSION     DAYYHX010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.376"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACHA6Y_002969"
     CDS             <1..888
                     /locus_tag="ACHA6Y_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM8988965.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          389 a          119 c          149 g          250 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcag
//
LOCUS       DAYYHX010000371          905 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000371 DAYYHX010000000
VERSION     DAYYHX010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.377"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>905
                     /locus_tag="ACHA6Y_002970"
     CDS             <1..>905
                     /locus_tag="ACHA6Y_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8988966.1"
                     /translation="KPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYPL
                     GTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVVT
                     LVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLLQ
                     YWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQSE
                     VGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGCD
                     KPKVSITVEFDKLTDSEEAKEFAKI"
BASE COUNT          336 a          115 c          182 g          272 t
ORIGIN      
        1 aaaccaagaa tatatagtcc tactgaaaca gatttctcaa cgaatggctt aggaatttta
       61 aaggattgta caagatgtgg aatatatgaa gtagctaatg gaaaatatga attagagttg
      121 gattatcctt taggaactag atttgatgaa tattttgaaa atgactatca aataaaagca
      181 aagccaaacg atcaagaaga gtatcatatc ttttttattg atgataaaga tatagatact
      241 tttttaaata cagtaactat ttatgctcag agtcgtacaa atcgacttgg aagacgggta
      301 gtcactcttg tggaagtgga ctctaaaact ggtcaagaag cgatatcaat tattgaaact
      361 aaaatggata aaaaatctga catacgactt tattctgata ttacggctgt ttctagcaca
      421 atctttgaag cgagaaatgt tttaaattgt attgctggtg aacaaggatc attgcttcaa
      481 tattggggcg gagaaattaa acgtgaacca tttaagcttt ctttgttaaa gcgaagaggt
      541 cgcaataatg ttggaacgat tcggtatgga aaagatatgt ctggtttgaa ggtcaaatta
      601 gattggacag gtataaaaac aagaattatt ccatatgctg atcctcaaag tgaagtaggt
      661 acgactaatc gaatttatgg ttcgccagta gatagtgcct atattaataa ctatcctgat
      721 gtctatacag aacatgttca gtttacagaa gaacaaggag taaaagacat taagagctta
      781 aataaaatag ctaaaaatta ctttaaaacg atcaatcctg gctgtgataa gcctaaagtt
      841 tctattacgg ttgaatttga taagttgact gatagtgaag aagcgaaaga atttgcgaag
      901 attag
//
LOCUS       DAYYHX010000372          903 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000372 DAYYHX010000000
VERSION     DAYYHX010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.378"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(239..637)
                     /locus_tag="ACHA6Y_002971"
     CDS             complement(239..637)
                     /locus_tag="ACHA6Y_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8988967.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(634..870)
                     /locus_tag="ACHA6Y_002972"
     CDS             complement(634..870)
                     /locus_tag="ACHA6Y_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8988968.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          287 a          160 c          122 g          334 t
ORIGIN      
        1 ctactttgta taatatatat tatgtaaact aaaatatata aaaaatcagc ccactagttc
       61 taaaccacta tttcctacta ttcactcttt tgtattcctt ttaaatcaat tatttcaaaa
      121 ttaatccata ttttccatat ttttatgaaa aaactatttt gtatgattaa acataaaaaa
      181 atcagcatat tccttaatga catgctgatc taattgagaa ataaagtttt taattcgttc
      241 attttattgc ggtttgtcgt aaataactgg ttatcttgga ctttagtgaa tcgaaatcca
      301 atgaactagt ggtaatcatt atgataaaat caacagcttc ttgacgagaa aaagcaactg
      361 gacaattatt caattgaaga aacaacaaca tggcaacaag agcagttcgt ttattgccat
      421 tatgaaaagg atgtttttta gctaaattaa ttgctaaaat cgccgctttt tcatagattt
      481 caggatagag ttcttgattg aatactgctt gtgaaggctg tttgacggcc atatctaatg
      541 catttgcatc ttttacaccg atcatttcgc ctggcgagtg acgtaaaata actttcgcgt
      601 tgattttgat aatttcttta gtcgaaagat acctcataaa tcaaccaaat cttttaacgc
      661 gtcttcatat tgattcatga tcgaatcaac taatgaatca atatcaggtt cttcttgtgc
      721 tttgatcatt cgtacagaac catcagattg aaaaacataa ctgattgcgt cacctgtctg
      781 cacacccatg aattctttta cttctggtgg gatagttgtt acatcgctat tgccagattt
      841 tctaatttta tactttttca tttttttcat atttcgccac cctctatacc attctcattc
      901 tat
//
LOCUS       DAYYHX010000373          899 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000373 DAYYHX010000000
VERSION     DAYYHX010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.379"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..>899
                     /locus_tag="ACHA6Y_002973"
     CDS             3..>899
                     /locus_tag="ACHA6Y_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic GMP-AMP synthase DncV-like
                     nucleotidyltransferase"
                     /protein_id="HFM8988969.1"
                     /translation="MDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDVKFETFLQG
                     SYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIFPNAQVNLK
                     VPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEADPKVLKEK
                     INSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLVNKSTDFLT
                     RKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYSKMTVNQMS
                     DFKNKLSKLYDDLIFAIDTEDEY"
BASE COUNT          330 a          120 c          174 g          275 t
ORIGIN      
        1 ttattgattt ccataattca attagattgg acgtagaaga caatacgcta cttaaggact
       61 ataaagacca agtaattgat ggattaaaag attacttacc tgatgatgtg aagtttgaaa
      121 cttttttaca gggaagctat tctgtttata ccggaataaa aagctgtgat gaaaaaattg
      181 attttgacat cgatattgca gttgctttcg aaattgatca tactgtttat gaagatccaa
      241 gggagcctaa actgtgggta aaagaggcgc tagttgaaat ttttcctaat gctcaagtta
      301 atttaaaagt accttgtgta actgctacat ttactggaaa aaagacaaaa aaaaatgtgc
      361 acgttgatgt tgcggtttat gctaaagaag atgaaaatta tttcttagca aaagcaaaag
      421 aattcagtgc tcccgaaaac cgatgctggg aggaagcgga tcccaaagta ttaaaagaga
      481 aaattaatag tcatgtagca gattcagatg atcgaaaaca atttcgaaga tgtattcgct
      541 atttaaaaag atggaaagat aacaatttta accaagaata taaaccaaca ggaattggac
      601 ttacaattaa tgtgatggat acgttcctag ttaataagtc cactgatttt ttaactagaa
      661 aagttcagta taatgatatg gaatgtatga agcagattgt ttcttcgctg aaagattcat
      721 ttgtgtatga gtacagtgaa acagatggtt ggcattatcg tctacatgca tatcttccag
      781 tgaagcctaa cagtgacacc tattcaaaaa tgacagtgaa tcagatgagt gattttaaaa
      841 acaaactttc taaattatat gatgatttga ttttcgctat cgatactgaa gatgagtat
//
LOCUS       DAYYHX010000374          898 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000374 DAYYHX010000000
VERSION     DAYYHX010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.380"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..>898
                     /gene="bsh"
                     /locus_tag="ACHA6Y_002974"
     CDS             6..>898
                     /gene="bsh"
                     /locus_tag="ACHA6Y_002974"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8988970.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAI"
BASE COUNT          306 a          122 c          164 g          306 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattga
//
LOCUS       DAYYHX010000375          897 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000375 DAYYHX010000000
VERSION     DAYYHX010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.381"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..434)
                     /locus_tag="ACHA6Y_002975"
     CDS             complement(<1..434)
                     /locus_tag="ACHA6Y_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8988971.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /gene="tnpA"
                     /locus_tag="ACHA6Y_002976"
     CDS             complement(451..855)
                     /gene="tnpA"
                     /locus_tag="ACHA6Y_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM8988972.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          270 a          145 c          142 g          340 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgttat atcaactcat ttcttgtata gactgattgt aacatgg
//
LOCUS       DAYYHX010000376          872 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000376 DAYYHX010000000
VERSION     DAYYHX010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.382"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..476
                     /locus_tag="ACHA6Y_002977"
     CDS             54..476
                     /locus_tag="ACHA6Y_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988973.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            486..689
                     /locus_tag="ACHA6Y_002978"
     CDS             486..689
                     /locus_tag="ACHA6Y_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988974.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
BASE COUNT          335 a          125 c          131 g          281 t
ORIGIN      
        1 ttaagattta ttaagaaaac gttttcttat ttttgatttc gaggtgatag acaatgaaaa
       61 ataagtttat tttatcttta ggtattttaa ctattggaat tagtgtaact ggaactacaa
      121 ttcctacatt tgctgatagt acaaatgaac caactactgt agtagataat aaccaagaat
      181 taactccttt agataggaac ggaatagcca ttggtaaccc aactaacgac ttaactgtta
      241 ctgaaaatta tttaatagtt tctccattac ttgctcgagc agcttctaaa acgatacgag
      301 tgacaggaac gatgattttt gataaagttg gttggccttc tagacctcct caatcacgga
      361 attggaaaga aaaaagaaat ggttattggt actatggaaa attgtattta caaagttata
      421 aatcagctgg aaacaattgg attgcgactt attcaggaac cttaactaga aagtagggat
      481 tttttatgcc agaatttgtg aacacaccaa atttttatca agatagcgat attctaagta
      541 aaattaattt accgaaagaa gaatcagctt tacaagttca gcgaacttta caggagacac
      601 aaaaaattca aaaatcaatt tctaatctag aagattctga agaaaaaaga aaaaaaagag
      661 accaaaaaac atatcaaaaa gaaaaataat gattttaaaa aaagagtgtc tagtcgacat
      721 tctttttttc taattgaaag tgttcataaa atcacccatt tctggacact ttcttttttt
      781 gctttatttt tcaaaaaaac aattcatttc atgttcataa acccataaca atatctatgt
      841 tcagttaaac tatatggata aagttatgtt ac
//
LOCUS       DAYYHX010000377          862 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000377 DAYYHX010000000
VERSION     DAYYHX010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.383"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>862
                     /locus_tag="ACHA6Y_002979"
     CDS             <1..>862
                     /locus_tag="ACHA6Y_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988975.1"
                     /translation="SGLQYDSLSEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNY
                     MSDFNDYVSSMITGQGSAGGYVVLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFS
                     KKGWRGPFNSAWTLDSIFNANFIQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKK
                     KIMELTKKGMEFWNSKGSIGTIGTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYIL
                     ISPKVGKGLVLLGNGKAIYFGDLDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSEL
                     KTDAEKRAWR"
BASE COUNT          316 a          107 c          178 g          261 t
ORIGIN      
        1 ttagtggact acaatacgat tctttatctg aaaaaacttt gaaattagaa gcaggagaca
       61 ttcaagttgc tttttatcag caacaagctg taactattca ggataaatta aaagattatg
      121 caacgaataa ttatatgagt gattttaatg attatgtttc ttcaatgatt acaggtcaag
      181 gcagtgcagg aggatatgta gttttatggc caaaagaaaa accttccaac atctttatta
      241 tggataatcc agatttagaa aaggcaagag aagtactaag aatgaataaa aatgggattg
      301 ctttttctaa gaaaggatgg cgggggcctt ttaattcagc ttggacatta gatagtatat
      361 ttaatgccaa ttttatacaa actggattaa tcaaagcaga tatctttcaa aattctttta
      421 acaagactgg agatgtattg aaattagtta atggactact ccaaatttgg aacaacaaaa
      481 agaaaataat ggagttaact aaaaaaggaa tggaattttg gaattctaag ggttcaattg
      541 gaacgattgg aacaactgat tctgttggta atccttttcc tggggcttct actccaaccc
      601 ctattgaaga taattcttta gttattcgta caaatggaga cggcaaatat attttgattt
      661 ctcctaaagt aggaaaagga ttagttttat taggaaatgg taaagcaatt tattttggag
      721 acttagatgt acaaggaaaa cttacagtta aaggaaaaga aataattcct ggacaaggtg
      781 gtggtccatc gggaggtgga gaatctactg gtgggtatcc tagtgaatta aaaacagatg
      841 cagaaaagag agcttggaga at
//
LOCUS       DAYYHX010000378          839 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000378 DAYYHX010000000
VERSION     DAYYHX010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.384"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            287..>839
                     /locus_tag="ACHA6Y_002980"
     CDS             287..>839
                     /locus_tag="ACHA6Y_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8988976.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPY"
BASE COUNT          324 a           87 c          162 g          266 t
ORIGIN      
        1 gagcgactcc tttggtaggg tgatgttttg gtcgactaaa ttttaccaaa agtgagtctc
       61 tttttgagtt gaaacgaaga aaaagtgtta gtagaaaatc gccttcgatt ttccaacaac
      121 acaaaatatt atagaagcct tttttctaaa ggatttcaag gtgtctgttt gcatttatta
      181 aaaaaaagaa aaagtattgg caacagaagg cgtcaccaac actagatatt atagagccat
      241 aatatgctgt ataatatcta gtgtataggc ttaagtggag gtatatatga aaatattaac
      301 tgttgaagat gataatatga taagagaggg aataagtgaa tatctttcag aatttggata
      361 tactgttatt caagctaaag atggaagaga agccttgtca aaatttaata gcgatataaa
      421 tttggttatc ttagacattc agataccttt tataaatggt ttagaagtgt tgaaagaaat
      481 tagaaagaaa agcaatttac caattctaat cttgactgca tttagtgatg aagaatataa
      541 aattgatgca tttactaatt tagtagacgg atatgtagaa aagccatttt cattgccggt
      601 cttaaaagct agaatagatt ctttaattaa gaagaattat ggacatttag agaagtttga
      661 atataaggat ctatcggtta attttaatag ctatacagct aaaataaatg gtgaagaaat
      721 agatgtaaat gcaaaagaac tagaagtatt aaaatgttta ttggataata atggacaagt
      781 gttaacaaga atgcaaatta ttgattatgt atggaaagat agcgaagaaa ctccttatg
//
LOCUS       DAYYHX010000379          802 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000379 DAYYHX010000000
VERSION     DAYYHX010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.385"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..693
                     /locus_tag="ACHA6Y_002981"
     CDS             <1..693
                     /locus_tag="ACHA6Y_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8988977.1"
                     /translation="ATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILS
                     DGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEK
                     VMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYK
                     ADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          295 a          125 c          148 g          234 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tactatattt ctttgttgtg taaagaagaa
       61 attatcgaat tacctaaaac taattccaca attggtattg acttaggtat tatggacttt
      121 gcgattcttt ctgatggaca aaaaattgat aataataaat tcacgtccaa aatggaaaag
      181 aaactaaagc gtgaacaacg caaattgtct aggcgtgctt tattggctaa aaataaaggt
      241 accaatctat ttgaagctaa aaattatcaa aaacaaaaac gcaaagtagc tagattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aatgatcaaa
      361 aatcacgata ttatctgtat tgaagactta aacacaaaag gtatgttgcg aaatcataaa
      421 ttagctaaaa gtatttctga tgtttcttgg tcaaagtttg tgactaaatt acaatacaag
      481 gctgactggt atggacgaaa aatcatcaaa gtagataaat ggtttccgtc tagtcagatt
      541 tgttcagagt gcggacataa agatggcaag aaatctcttg aaattcgaga atggacttgt
      601 cctgtttgtc atactcatca tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa
      661 ggtctaagac tacattctat gggattggct tagatccaat catcaagaac cgtaggaact
      721 acggggatag cttggtaaat aagagacacc gctgttagta aagaaatacg ctatcaagta
      781 tgctctattc ccaagaagct cc
//
LOCUS       DAYYHX010000380          802 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000380 DAYYHX010000000
VERSION     DAYYHX010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.386"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            418..>802
                     /locus_tag="ACHA6Y_002982"
     CDS             418..>802
                     /locus_tag="ACHA6Y_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8988978.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYN"
BASE COUNT          277 a          149 c          121 g          255 t
ORIGIN      
        1 gttctttatt aattaaaaat gttctgtaat aaaataaaac tttataacaa ctataacaaa
       61 accgaatgca aaacagaagt tctataagtt ataatcatag ttgttaggtg attcatctag
      121 taaaaacacc agatttcaaa tttcacacaa tttaaactag atcaatcatc aaccttcttc
      181 aaattgttag aaccctttcc cgaggagctg cagcataagc aaaaaaacaa acaggaagcc
      241 aaatggctaa gacatttttt ctattagtct agttgcttct cgtcgcaagt ttaagacttt
      301 agaaagttat ttctaatgat gtttgggttg ttttgatcaa tgcttatgca cagctcctta
      361 cctattattt atctcaacag atctaacaaa ttaatggact atttaaggag tttttttatg
      421 cttacgatct attcttctcg cacacatagt taccatctga tggcaaactg gctgaaagac
      481 catcaaatgc cttacagaga acgaattttc acacctaaac agcctttaac aaggaaagag
      541 atattttatt tattgtcatt aacagaaaac ggatttgacg atctattagc taaacgctcg
      601 attgattata aaaatttgaa agaccaaatc gaccaatgta cgacctcaga gcttgtcgat
      661 ctgatcgtct cgcattttac tttgttacaa cgtcccatca ttactgacgg aaagaaatta
      721 ttagtcggat tcaatgaaga tagcatgcgt ctttttatcc ctgtacaatg gcgcagaagt
      781 caactgaaat ctgtctataa cg
//
LOCUS       DAYYHX010000381          798 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000381 DAYYHX010000000
VERSION     DAYYHX010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.387"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..636)
                     /locus_tag="ACHA6Y_002983"
     CDS             complement(82..636)
                     /locus_tag="ACHA6Y_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8988979.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          243 a          156 c           84 g          315 t
ORIGIN      
        1 acttccgaaa attcagttat cttcattaat ctcaccttct aagttttcat tatacacaaa
       61 aaatatatcc taatatctca attagcctcc caatttaaaa ccacgtaacg atgcccctat
      121 ttcatcttca gttataccaa tgtacctttt tgtaattgct tgcgagctat gattaaaaat
      181 aaacattaag gtagcaatgt ctctagtttt cttgtagtag tgatacccaa aagtttttct
      241 taatgtatgt gttcctatat catctcttcc taatttttct gctacatctg catatatctg
      301 atagacacga tcccttgtta tatgtcgacc ttgttgccga cttggaaata accaatcatt
      361 ttcttcttta ccttcagtat ataataaaat ctcttctctc aaattatcta taaaaatcgg
      421 ttttcgctta cctgttttat gttcaacaat aataggctta cttttacctc gtatgtctcc
      481 aacttttaat cctataatat ctgacatacg taacccatta ttgattccaa acaaaaataa
      541 aaaactatct ctttctggat tacttgttct tcttagccaa aactgaaaat cttgtatctc
      601 ttcatttgtc cttaacggtt gtacattata gctcactatt gtttcccttc ttaaaaatat
      661 tctgagttca tgattatata tatattttac cataatcatg aacttatata tatactctgt
      721 taaaagtgaa gaaaatcaag gggtttagtt tatgatttta tatcatttca gtaactcaga
      781 atattccgac ttttttgt
//
LOCUS       DAYYHX010000382          781 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000382 DAYYHX010000000
VERSION     DAYYHX010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.388"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..288)
                     /locus_tag="ACHA6Y_002984"
     CDS             complement(<1..288)
                     /locus_tag="ACHA6Y_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8988980.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPER"
     gene            complement(294..524)
                     /locus_tag="ACHA6Y_002985"
     CDS             complement(294..524)
                     /locus_tag="ACHA6Y_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988981.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
BASE COUNT          277 a          134 c          106 g          264 t
ORIGIN      
        1 tctttctgga tcaacgaaag tatactttat atagtcataa cgccgatgat cgcttcgagc
       61 gtctagcaca tcagtcacga tatcaaacaa aaaatatacg tctttcttca ttctagtttt
      121 cgcagcaggg attttaaagt agttcttatt agaatagtag agattaatta ataagctatc
      181 ttcgattgct aaaaagaaaa cttctgaatc ccacacctta taaaaatctt tgacaaatct
      241 attcgaagga tcaaatttaa accataattg cgtctcatta aaaagcataa ccattactcc
      301 gatctatttt taaacttagt ctctacctct aatatatatc gagtttttat tttgccttca
      361 gagaatactg tttctttttt tgcagttaca ggttgtttat tttcggaaaa agctaatata
      421 gctaaaattg aaacatccat ctggaatttg tctttttcgt tgctttgctc ataaaaatct
      481 gcatattcat cactaatatt ttttctaata aattcttcca tcataaagat cacctcgaaa
      541 agagtatacg aacaaacgtt ctttttgtaa aggtggaatt tattatacta aataaatagg
      601 tgaacaagta cttgtgccaa attatgtgcc aaaaaaaatc gaatttaata aaacacaaac
      661 aaaaagaaat cctattatga taacatttct tataataaca aacacaacag aagacgtgta
      721 atagttagtc aggaacgtac aaataacccc tgtatccttt gcggtacagg gggttatttt
      781 g
//
LOCUS       DAYYHX010000383          752 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000383 DAYYHX010000000
VERSION     DAYYHX010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.389"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..549
                     /locus_tag="ACHA6Y_002986"
     CDS             <1..549
                     /locus_tag="ACHA6Y_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8988982.1"
                     /translation="DFYEEFYKRLMRFIFVTGVRSEELLALQWKDFDLINGRCSVNKS
                     IYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLKTWKKTQQSIGKNIELVFSFDGLPP
                     SPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFLIEHNKNIYAISKRLGHASIKTTLD
                     KYGHLYPETNQALANEITRFDI"
BASE COUNT          262 a          114 c          137 g          239 t
ORIGIN      
        1 gatttttatg aagaatttta taaacgtttg atgcgtttta tttttgttac aggtgtaagg
       61 agtgaggagc tcttagcgtt acaatggaaa gactttgact taataaatgg cagatgtagc
      121 gtaaataagt caatttacat ccgtacaaga caagattatg aatttgctga aacaaaaaat
      181 acatcaagta ttcggataat ttcgttagac agacaaaccg ttgaagattt aaaaacatgg
      241 aaaaaaactc aacaatcaat tgggaaaaat atagaattgg ttttttcttt tgatggatta
      301 ccgccatctc caagaactat tttaagcaga ataaaaaaac tagctgaaat tgcaggagta
      361 aaaccaattc atatgcatgg attaagacac tcgcatgttg cctttttaat tgaacataat
      421 aaaaatatct atgcaatatc taagcgtcta ggtcatgcct ctattaaaac tactttggac
      481 aaatatggac acttatatcc agaaacaaat caagcactcg caaatgaaat tacgaggttt
      541 gatatttaac tttccatctt ttttgctgtc acgctgctgt catagcctat ttttattttt
      601 ttatgttgat ataccttggg ttataagcta gctgtcggta tcgaaaatgg acgaggacga
      661 accgaagaaa tagcaagggt ttgtttgaaa acagtgtaaa aaataaacca tgtttacatg
      721 gatttgccac cggtttgcca ccggtggttt tt
//
LOCUS       DAYYHX010000384          736 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000384 DAYYHX010000000
VERSION     DAYYHX010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.391"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..636
                     /locus_tag="ACHA6Y_002987"
     CDS             133..636
                     /locus_tag="ACHA6Y_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8988983.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
BASE COUNT          212 a           97 c          193 g          234 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcctactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatgcgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cctgtcgatt ttgatgaagt gaaagaacag
      241 attggattaa atgacgaata tgaagagtac gcgattcatg attatgaact gccttttgag
      301 attgatgagt atacgccaat tgaagagatt aatcggttgt gtaacttagc ggaggaacta
      361 gaaggaacgc caattggtga agctgcttct gagattcaac acgctttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatt gtctattatc cagattgcga tgatatgagt
      481 gatgtggcgc attacttgat tgtagaagct ggtgattttg gcgaagttcc agaacaattg
      541 aagccgtata ttgattttga tgcttgcggt cgagatttgg agattggagg aaattatttg
      601 gttactagtc gtggagtttt tgagtatcca gaatgaatca aacactatat atagtgtttg
      661 attagtatat gtaagcaaaa aaacacaata tatagagtgc aaactgaagg gtaatgtgat
      721 ataatatttg ccgaag
//
LOCUS       DAYYHX010000385          707 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000385 DAYYHX010000000
VERSION     DAYYHX010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.392"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..>707
                     /locus_tag="ACHA6Y_002988"
     CDS             142..>707
                     /locus_tag="ACHA6Y_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8988984.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLK"
BASE COUNT          248 a          103 c          116 g          240 t
ORIGIN      
        1 tctcagggca attctaagaa cgtttcaaga gatagcgaca ctatcgttat cgaaaaaggc
       61 gaaaacctat ctcattttct gctaatgtaa gttggtttag cttttgttaa tatgacttta
      121 tgaaaatgga ggtaattaaa tatgattttt cctaaagtta atgaagcaag cttgaataaa
      181 ggatcctttt ttcatatcta tgaaaacgaa gattttgaac tgtataatat cctttcacaa
      241 aaagaggtca tcgaaaacca aataatctgc catccaatta tttacaaatc tattttcaag
      301 tatgcatttt ttgcttccaa tgattttcaa agaatggaga ttaccgactg tatattcgaa
      361 aactgtgatt tttcaggctg tatattgaca aaaagcatgt tgcatcgcgt gaaatttgtc
      421 aattgtaaat taacaggtac taaattgatg gaaagttata ttgggaacac gttattcgag
      481 aattgtaaaa tggactatgt aaatttatct ggttcaaaca taaaagaaag tacctttgag
      541 cactctgtct tatcaagcgc agattttgta gactgttccc tcactaaaat aatgttttct
      601 actaatgatt tagaaaatgc tagctttttt gatactgatt taaaaaatat cgatttaagt
      661 agcaactcct ttgaaatgat tgaagtacgc tcggaacatc taaaaaa
//
LOCUS       DAYYHX010000386          706 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000386 DAYYHX010000000
VERSION     DAYYHX010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.393"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..258
                     /locus_tag="ACHA6Y_002989"
     CDS             <1..258
                     /locus_tag="ACHA6Y_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988985.1"
                     /translation="KGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            260..397
                     /locus_tag="ACHA6Y_002990"
     CDS             260..397
                     /locus_tag="ACHA6Y_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8988986.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
     gene            435..>706
                     /locus_tag="ACHA6Y_002991"
     CDS             435..>706
                     /locus_tag="ACHA6Y_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8988987.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          273 a          100 c          142 g          191 t
ORIGIN      
        1 aaagggaatg ctgttgatag cgtagatgaa ttggctaata ttaaggcatt atacgaaacg
       61 ctgaaggcag aaaatgatga attgaaacag ctaaattcta aagcaatgct aaacaatgta
      121 gcaatcaagc aagaaaatgt cttattgaaa gaaaagtcag aaagtttagc gcagttgaat
      181 tcaaaaacga tgcttgcttc tgtacaaaat accaaggaaa tcgaagaaat caaaaaacaa
      241 ttacaaggtg ggaagtaaca tgtattcata tgaagatatc aaactaatgt atgactgggg
      301 ctgtttcact aacgaacaag taatggtttt cgttccgttg tgcattactg aagaagaagc
      361 agataaaatc attagcaaag aagagagcgc atcttaattg atgtgctttt tattttgatt
      421 caaggagttg tcacatgatt aatttagggg aatggggagc gatagcagga tcaataaccg
      481 ctatcgtttc tttgatttta ttagtaatta gaccgattgc tgcatctttc tcgaaaatta
      541 ctgagactct ttcaaaagta agccacaatt tagatttgct gactaaagat ttagaatcga
      601 gcaaatcaga tcgattgatg attcatgaag aactaaagaa acacgatgaa agattagata
      661 cacatacaga aaaattggtg gaacacacgc aacaaatcaa aacttt
//
LOCUS       DAYYHX010000387          706 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000387 DAYYHX010000000
VERSION     DAYYHX010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.394"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..189)
                     /locus_tag="ACHA6Y_002992"
                     /pseudo
     CDS             complement(1..189)
                     /locus_tag="ACHA6Y_002992"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            complement(315..>706)
                     /locus_tag="ACHA6Y_002993"
     CDS             complement(315..>706)
                     /locus_tag="ACHA6Y_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HFM8988988.1"
                     /translation="FLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSERE
                     VVRDNAGLMAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQ
                     MRDELSELAKKVKVTFLTDPRWVRIDK"
BASE COUNT          239 a          145 c           66 g          256 t
ORIGIN      
        1 ctcacttaaa aaatatgccg aaataaatat aaaacaatta actcctagct gaccccaaat
       61 accaatataa ttcaagaaaa tctggttagc agttaatgaa ccatgtacta taattccgtc
      121 tgcattataa tttaaacgca aattagcatg gtaagcaaca atacacaata tagaaatgat
      181 tcttaaaagt tcaaaattag agtttcatct cgccccatcg gcccttaaat atttttcttc
      241 ttttttattt aagactttac ccatttttat tctctcacta tctctttatc aaaattttaa
      301 aaacattatt actactactt atcaattcga acccaacgtg gatccgttaa aaatgtcacc
      361 tttacttttt tagctaattc tgacaattca tctctcattt gcacgttcat tgcatctaat
      421 aatgcaaatt ttgttataat ttccatggat tgctcaccat aattctcatc ttcattatgt
      481 gaatatccat caaatccagc taagaatatt tcatccacat tcatatcggc taaaaatcta
      541 attgccatta atcctgcatt gtctcttaca acttcccttt cagataacaa ctttttataa
      601 ctcgtccgta aatatacatc cgatgcttgt atatttgatg taacaataca tttagttctg
      661 ctttctttcg gtaattcatg aaaacgtctt aaattactta aaaata
//
LOCUS       DAYYHX010000388          701 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000388 DAYYHX010000000
VERSION     DAYYHX010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.395"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..623
                     /locus_tag="ACHA6Y_002994"
     CDS             <1..623
                     /locus_tag="ACHA6Y_002994"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HFM8988989.1"
                     /translation="YKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDI
                     SECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDF
                     SCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNID
                     IEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          307 a           44 c          119 g          231 t
ORIGIN      
        1 gttataaaga aattaaagga acttttttaa caccagaaat ttattctact atgtcagaag
       61 aagaacaaaa tttgttaaaa cgagatattg ccagtttttt aagacaaatg cacggtttag
      121 attatacaga tattagtgaa tgtactattg ataataaaca aaatgtatta gaagagtata
      181 tattgttgcg tgaaactatt tataatgatt taactgatat agaaaaagat tatatagaaa
      241 gttttatgga aagactaaat gcaacaacag tttttgaggg taaaaagtgt ttatgccata
      301 atgattttag ttgtaatcat ctattgttag atggcaataa tagattaact ggaataattg
      361 attttggaga ttctggaatt atagatgaat attgtgattt tatatactta cttgaagata
      421 gtgaagaaga aataggaaca aattttggag aagatatatt aagaatgtat ggaaatatag
      481 atattgagaa agcaaaagaa tatcaagata tagttgaaga atattatcct attgaaacta
      541 ttgtttatgg aattaaaaat attaaacagg aatttatcga aaatggtaga aaagaaattt
      601 ataaaaggac ttataaagat tgattatata atatatgaaa agctattata aaagacatta
      661 gtattaaata gtttaaaaaa atgaaaaata ataaaggaag t
//
LOCUS       DAYYHX010000389          694 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000389 DAYYHX010000000
VERSION     DAYYHX010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.396"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..481
                     /locus_tag="ACHA6Y_002995"
     CDS             8..481
                     /locus_tag="ACHA6Y_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM8988990.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            490..>694
                     /locus_tag="ACHA6Y_002996"
     CDS             490..>694
                     /locus_tag="ACHA6Y_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8988991.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKND"
BASE COUNT          219 a          112 c          128 g          235 t
ORIGIN      
        1 gataaaaatg aaatttaaaa cattgctatt aaaatttgtc gttttaggta taagtgccgt
       61 atttattttg ttcggcgtac ttttttatac tcagatcgct acaagcgaaa aaggattcaa
      121 ttttgattgg cagaccacat tatttgtttc tgtcatttac tttgcccttc tcctagggat
      181 cgttattgct tatttcttat tgaaactatt gaaaaaaatc gaagataacc aagctttttc
      241 cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc attttgttta ttggtttcgc
      301 cttttttggc ttacttccta aatttatgaa agtagcggat ttagacgatg ctccaggttt
      361 gatgttggta ggaatcgctg tcgtattcat tccttttgca gtatacacat taacaagcgt
      421 gctggaaaaa ctgcttgaac atgcgatttt acttaagaca gatcatgatt tgacagtgtg
      481 aggtgcgaca tggcaattat cgttaattta gatgtagtat tagctcaaaa aaaaatgagt
      541 gtgactgaat tgagtgagaa agtgggcatt acgatggcaa acatctctat tctaaaaaat
      601 ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa tctgccaagt tttggagtgt
      661 caaccaggtg atttgttgac ctataaaaat gact
//
LOCUS       DAYYHX010000390          693 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000390 DAYYHX010000000
VERSION     DAYYHX010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.397"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..627
                     /locus_tag="ACHA6Y_002997"
     CDS             <1..627
                     /locus_tag="ACHA6Y_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM8988992.1"
                     /translation="LGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITGFIAF
                     TLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPINEAF
                     SPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNGNILH
                     TVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          209 a          115 c          114 g          255 t
ORIGIN      
        1 ttaggaaaaa aaatagaact taaaaaaggg atattgggca ctattgtcgt tgtactcggt
       61 ttgttagggt tgctatttgc gccatccatt attagcattt tttctttaac tatcactgga
      121 tttatagcat ttacacttgt ttttggtaaa aaagatacga aaaaaatgtt ctcgaaacct
      181 gtcgctcctg tcaaaaatat agccaaatat ttttttatca atgtagtaat cagtgccgca
      241 gtctcggtat ttttgcaaca aattttgaaa tggggtttaa caggcaatcc aataaatgag
      301 gctttcagcc cgttattatt tattattttg cctatcatga tacttggaga agaattgttc
      361 tcagtgtatt ttctcgctat tttttctagt aagttcagca ttcctgtcgc tagtttcttg
      421 tcagcaatca tctttggatt tatccattac agtacttatg ataatggaaa tattttacac
      481 actgtagcac acatcctttt gatacaaggg gtggcaagat tgttattcaa ccaagctgcc
      541 attaaaagca actcgatcat tacaagttgg gcagttcatg tgatatttga tttcacagct
      601 atattgctag tcgttttatt ctcctaaatt tttatagaaa taatacatgc tctattgcta
      661 agaaagcata ctttatttcc tttaaacaaa aag
//
LOCUS       DAYYHX010000391          691 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000391 DAYYHX010000000
VERSION     DAYYHX010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.398"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..316
                     /locus_tag="ACHA6Y_002998"
     CDS             155..316
                     /locus_tag="ACHA6Y_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM8988993.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            335..>691
                     /locus_tag="ACHA6Y_002999"
     CDS             335..>691
                     /locus_tag="ACHA6Y_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8988994.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSL"
BASE COUNT          267 a           73 c          127 g          224 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcag gataaggata agtgtattaa
      181 ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat tcagttgaga attgcaatca
      241 tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt ccagtggatg caattagtta
      301 tggagtaaga aaatagaaag gagtagttag gaaagtgata aaaaatgcta tagcatatat
      361 tacaagaaag aaaaatagaa ctttaataat ttttatcata ttaactatag ttctttcttg
      421 cttgtattct tgtttaacaa taatgaaatc aagcaatgaa atagaaaagg ctttatatga
      481 aagttctaat tcttcaatat caataacaaa aaaagatggt aaatatttta atgttaatca
      541 atttaaagat attgaaaaat taaaagaaat tgaagaaaaa ataattcaat atgatggatt
      601 agcaaaacta aaagatgcta aagtagttag cggagaacaa agaataaata gagaagattt
      661 atctgacgaa tttaagaatg ttgtttcact c
//
LOCUS       DAYYHX010000392          679 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000392 DAYYHX010000000
VERSION     DAYYHX010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.400"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..562)
                     /locus_tag="ACHA6Y_003000"
     CDS             complement(<1..562)
                     /locus_tag="ACHA6Y_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFM8988995.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDE"
     gene            complement(567..>679)
                     /locus_tag="ACHA6Y_003001"
     CDS             complement(567..>679)
                     /locus_tag="ACHA6Y_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFM8988996.1"
                     /translation="CKELLVEKGYFFYRNSKIKRIPISILEEVLHISWEE"
BASE COUNT          211 a          129 c           81 g          258 t
ORIGIN      
        1 attcatcaac tgtccaaaat tctacttgag gacgctgagt ttttatttta cctatagtat
       61 ctacaggatt ttcttcaata atttcaagta caattgccct tttaaaaact actctcaact
      121 tattgaatat ctgtttaata taatttggtg ataatggttg ctcaatgccc ttttttacaa
      181 catggatttt cgataaaaac tgttgaaatt cctgtatatg aattggtcga atctttacaa
      241 cctgcatttc tccaaaaaaa tccaatgcac gttgtaaaat atgatctgtt ttcatatatg
      301 ttttttctac agttcccaac ttataccaag gaaaaaaata ctctcgataa aaatcatgaa
      361 acgttattcc ttgcagataa attccttgtg aatcagaaaa actttgtttt aactcttcga
      421 gtccccttct agcttctcct tgagttttga agcctcttct ggtcacttga attctttttc
      481 caaagcgatc ttgtcctaaa gttgcagaaa aaaaccaagt gcctcttttt ctgtctttat
      541 aaacattcgc aattttcctc attttactat tcctcccaag aaatatggag aacttcttct
      601 aaaattgaaa taggaattct ttttattttt gaattacgat aaaaaaagta tcctttctca
      661 actaaaagtt ctttacatt
//
LOCUS       DAYYHX010000393          673 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000393 DAYYHX010000000
VERSION     DAYYHX010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.401"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(88..672)
                     /locus_tag="ACHA6Y_003002"
     CDS             complement(88..672)
                     /locus_tag="ACHA6Y_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8988997.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          209 a          156 c           98 g          210 t
ORIGIN      
        1 ttatcagtag acacttacaa aaaattctca tactattgtc acttttctag ataaatggca
       61 aaaattttca gcattaaatg acaaaaacta atgagaattt gtctttccat ttttctcctc
      121 catttttgcc aaataacgat aaatggtggt tcttgagacg ttccaccgtt ccgcaatagt
      181 cttaataggt gtaccgctat ccactaaagt cttcagtaat tccaaatctt gttcagacaa
      241 tttttcagga cgaccaccaa accgtcctct tgcacgagca gctgctcgtc ctgctgcgga
      301 tcgttccagg atcaaattac gctcaaattc tgcaaatgcc gcaaacaaat gaaacatgag
      361 ctgtcctgta gaacttgatt tgtccatcgt aatattttct tgaatactat gaaaactcac
      421 accacgctca ttcagtcgat tgacgattga aatcaaatct tccatatttc tacctaaacg
      481 atctaagcgc caaacgacaa gtgtatcgcc agagcgaaca aaatcaatgg ccatttctaa
      541 tcctggtcga ttactttttg ccccactcat atgatcagta aaaatttttt cacaaccaaa
      601 tttatttagg ctgtcttctt gtaaatccaa attttgtaaa ccagtagata cccgggcgta
      661 tcctattttc aaa
//
LOCUS       DAYYHX010000394          666 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000394 DAYYHX010000000
VERSION     DAYYHX010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.402"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>666
                     /locus_tag="ACHA6Y_003003"
     CDS             <1..>666
                     /locus_tag="ACHA6Y_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8988998.1"
                     /translation="EHTQRGFHALLEEIQHLPERPLIVFEATGIYSRPVEKFFQDHHF
                     SYCLLNPLEAKKQMEENTLCSWKTDKSDAHRLAQTHLKNQRQPKEVQSNFYLEMRDLA
                     RFYQEIEKKITRLRMDLHNCLQLTFPELEQFFLNRLTPYALILIRLFPHPDFVLASTR
                     TKIKNKLINETRKKISANRAEQKADQIIHYAQCAYPAVEKDSIHCQKTIYYAELLQDL
                     LEQK"
BASE COUNT          227 a          149 c          116 g          174 t
ORIGIN      
        1 gaacacactc aaagaggatt tcatgcatta ctcgaagaga tccagcactt gcctgaacgt
       61 ccattgattg tttttgaagc aacggggatt tattccagac cagtcgaaaa gttctttcaa
      121 gatcatcact tttcttactg tttgttaaac ccgttagaag cgaaaaaaca gatggaagaa
      181 aacaccttat gcagttggaa aacagataaa tcggatgccc atcggctcgc acagactcat
      241 cttaaaaatc aacgtcagcc aaaagaagta caaagcaatt tttacttaga gatgcgcgat
      301 ttagcccgtt tttatcaaga aattgagaag aaaatcactc gtctgcgcat ggatttacac
      361 aactgccttc agttgacttt tccagagctt gaacagttct ttttaaacag gctgacccct
      421 tacgcattga tattgatccg tctttttccc catccagatt ttgtattggc atctactcgg
      481 acaaaaataa aaaataaact gatcaacgaa acacggaaaa aaatctccgc gaatcgtgcc
      541 gagcaaaagg cagatcaaat catccactat gcacagtgtg cctatccagc tgttgaaaaa
      601 gacagtatcc actgtcaaaa aacgatctat tacgctgaac ttctccaaga tttactcgaa
      661 caaaaa
//
LOCUS       DAYYHX010000395          666 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000395 DAYYHX010000000
VERSION     DAYYHX010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.403"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>666
                     /locus_tag="ACHA6Y_003004"
     CDS             <1..>666
                     /locus_tag="ACHA6Y_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8988999.1"
                     /translation="EHTQRGFHALLEEIQHLPERPLIVFEATGIYSRPVEKFFQDHHF
                     SYCLLNPLEAKKQMEESTLRSWKTDKSDAHRLAQTHLKNQRQPKEVQSNFYLEMRDLA
                     RFYQEIEKKITRLRMDLHNCLQLTFPELEQFFSNRLTPYALTLIRLFPHPDFVLASTR
                     TKIKNKLINETRKKISANRAEQKADQIIHYAQCAYPAVEKDSIHCQKTIYYAELLQDL
                     LEQK"
BASE COUNT          225 a          154 c          118 g          169 t
ORIGIN      
        1 gaacacactc aaagaggatt tcatgcatta ctcgaagaga tccagcactt gcctgagcgt
       61 ccattgattg tttttgaagc aacggggatt tattccagac cagtcgaaaa gttctttcaa
      121 gatcatcact tttcttactg tttgttaaac ccgttagagg cgaaaaaaca gatggaagaa
      181 agcaccttac gcagttggaa aacagataaa tcggatgccc accggctcgc acagactcat
      241 cttaaaaatc aacgtcagcc aaaagaagta caaagcaatt tttacttaga gatgcgcgat
      301 ttagcccgtt tttatcaaga aattgagaag aaaatcactc gtctgcgcat ggatttacac
      361 aactgccttc aattgacttt tccagagctt gaacagttct tttcaaacag gctgacccct
      421 tacgcattga cattgatccg tctttttccc catccagatt ttgtattggc atctactcgg
      481 acaaaaataa aaaataaact gatcaacgaa acacggaaaa aaatctccgc gaatcgtgcc
      541 gagcaaaagg cagatcaaat catccactat gcacagtgtg cctatccagc tgttgaaaaa
      601 gacagtatcc actgtcaaaa aacgatctat tacgccgaac ttctccaaga tttactcgaa
      661 caaaaa
//
LOCUS       DAYYHX010000396          656 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000396 DAYYHX010000000
VERSION     DAYYHX010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.404"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>656
                     /locus_tag="ACHA6Y_003005"
     CDS             <1..>656
                     /locus_tag="ACHA6Y_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="E2/UBC family protein"
                     /protein_id="HFM8989000.1"
                     /translation="KEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHFPLVLPRI
                     YIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTIEEGLKGL
                     NKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKENELQLNAA
                     SRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTGANKRFFL
                     "
BASE COUNT          245 a           71 c          123 g          217 t
ORIGIN      
        1 taaaagaaaa gctaacagat gttgtgcgtt tattcgatgg taattactat ggggattttt
       61 catttgctac aattggaaag ttgcgtttaa aaattgaaat accaaaacat tttccattgg
      121 tacttcctag gatttacatt cataattttg atcatttagg actttaccta ccacatgtag
      181 aaaaagatgg gttggtatgt ttttcttcag atataaatta tttagtagat gaagaaaatt
      241 cagaattggt tttattgcag tgtgttaata aaactataaa aacaattgag gaaggtttga
      301 aagggttaaa taaaaatgat tttcgaaaag agtttgttgc tttttgggac aaacaagaga
      361 atcaaatttc gttattctac ctttgtgaac cagcgttgtt tatcaagtca gttgatacaa
      421 taatgtacgg taagtctttg gttgctttgg ataaagagaa cgaattacaa ttaaatgcag
      481 cgagtagatt gtttaatgga gagaaagaag atactcatga attagatgca tactatgttc
      541 caattagaaa caagaataat attatgcccc cgaagaataa taaagcatgg tcaaagaagg
      601 atattttaca aatagtaaaa aaaaatgtaa caggtgcaaa taaaagattt ttttta
//
LOCUS       DAYYHX010000397          645 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000397 DAYYHX010000000
VERSION     DAYYHX010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.405"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..292
                     /locus_tag="ACHA6Y_003006"
     CDS             <1..292
                     /locus_tag="ACHA6Y_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8989001.1"
                     /translation="RLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRK
                     QEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            364..>645
                     /locus_tag="ACHA6Y_003007"
     CDS             364..>645
                     /locus_tag="ACHA6Y_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8989002.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSL"
BASE COUNT          275 a           85 c          104 g          181 t
ORIGIN      
        1 aagattagaa cctatggata gaataaaaga attgagtagt gcatcggttc catttgaatt
       61 tgacgttcat gcgatgattt tttcagagga cgctccgtct ttggaaacta aattgcataa
      121 tcattttaga aaacaagaag ttaataaaat taatcaaaga aaagaatttt tcaaagtttc
      181 actagacgaa atagagaaag ttgttttaga aaattataat ggaacagtaa catttacaaa
      241 attagctaaa gctgaacaat atagacgatc tttagaattg tcaaaagatt aaatatataa
      301 aaagccttcg ggctttttct tttaacaatt aataggaacg tatattcgaa aggaatagaa
      361 ataatgtggt tagaaaaaat aaatgacaaa aaatttaagt acaccgaacg ttatacggat
      421 cccttaactg aaaaaaagag aaaagtgtcc gttactttat ctagcaattc acgccaagct
      481 tggaatcaag ctaacttact tttgaatgaa aaaatcgcag aaaaaataaa acgaaatgaa
      541 gaattaccac taacttttgg agaattaaaa actaaatggg atgaaaaata caagccaact
      601 gtaaaagaaa gttcctatcg aacaactcag gtttatctat catta
//
LOCUS       DAYYHX010000398          637 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000398 DAYYHX010000000
VERSION     DAYYHX010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.406"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..>637
                     /locus_tag="ACHA6Y_003008"
     CDS             3..>637
                     /locus_tag="ACHA6Y_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8989003.1"
                     /translation="MCGIILGAGIYGLAVTGINIPSKLADGGVTGIALLLHNLFGFAP
                     SVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFAVDSLLINSL
                     MTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGILVVSLLYLD
                     LTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLEQL"
BASE COUNT          157 a          115 c          121 g          244 t
ORIGIN      
        1 aattgtgcgg aatcatttta ggcgcaggaa tctacggatt agctgtgaca ggaatcaata
       61 ttccatctaa gctggcagat ggtggggtta ctggtattgc tttgcttttg cataatcttt
      121 ttggattcgc tccttctgtc acttcactgc tttttaattt acctttattg cttatctcat
      181 tgtttctttt tggaaaacac gcttttctcc ggacgatcgt cggaacattt gcgctagttt
      241 tctttttaca tttatgggag agccttcata tacattttgc tgtagatagt ctactgatca
      301 atagtttgat gacaggtatt ctttctggca ttggttgcgg tctcgtcttt cgattcggcg
      361 gaagcacagg cggaacagat atcatttatc aagccattga aaaatatttt catattaaga
      421 ttggcaaaag tcttttcttg attacttttg gtattttagt tgtctccttg ctttatttgg
      481 atctcacaca ttttctgtat acattactta gctgttctat tctatcttat acattgaata
      541 aagtgaagta tctggaaatt ggatctgttt tgccccaatt ggaagcaaaa cagactacag
      601 aaacatttga accacttgat cctcttgaac agttaga
//
LOCUS       DAYYHX010000399          628 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000399 DAYYHX010000000
VERSION     DAYYHX010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.407"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..>628
                     /locus_tag="ACHA6Y_003009"
     CDS             23..>628
                     /locus_tag="ACHA6Y_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM8989004.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPHFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIE"
BASE COUNT          229 a          114 c          118 g          167 t
ORIGIN      
        1 aacaaaggag tgtaacccat aaatgaccta caaacatctt accatagacg aactgacaat
       61 gatagaatca tattatcttc aacataataa gccggttgaa atcgctaacc gaatgggacg
      121 tgctatacaa actatttata atgtagtcaa taagttcaag caaggcaaga ctgctcttga
      181 ttattggcac cagtataaag aaaataagaa aaaatgtggt agaaaagtca ttcaattacc
      241 tgctcatgaa gtagattaca ttaaagagaa agtcactctt ggttggacgc ctgacgtcat
      301 tatcgggcga aaagaaaggc ctgtttcatg cggtatgaga acactttatc gtttattttc
      361 taaaggaata tttgatattg acacactacc gatgaaaggt aaaagaaaac ccaatggcca
      421 tcaggaaaaa cggggaaaac aacagtatca gcgctcaatc catgatcgac ctgataatta
      481 tcctcatttc aattctgagt ttggtcacct tgaaggtgat acgatcgttg gcattcatca
      541 taaaagtgcc gtcattactt tagttgaaag attatctaaa gtcattatca cgattaaacc
      601 caacggccgt aaggcattag atattgaa
//
LOCUS       DAYYHX010000400          627 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000400 DAYYHX010000000
VERSION     DAYYHX010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.408"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..315
                     /locus_tag="ACHA6Y_003010"
     CDS             115..315
                     /locus_tag="ACHA6Y_003010"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8989005.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            327..>627
                     /locus_tag="ACHA6Y_003011"
     CDS             327..>627
                     /locus_tag="ACHA6Y_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8989006.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECK"
BASE COUNT          269 a           64 c          117 g          177 t
ORIGIN      
        1 agcatgaaaa ccacaaattg agtaatattt taattgatat tactcgtttt gtggtttaaa
       61 atgtgcttat taagataaaa accacaaagt gaataatttg aaaggaggcg ctgaatgata
      121 actcttaatg atcaattcat tagaagctta agaagacata gagccgatct tatacttact
      181 aaaaacgatg cagcaaaatt gataggtata aatcggaaaa catatgtcaa aatcgaaaat
      241 gggagcaaag aaagtattag agcatctact tatcaaaagt tggtaaattg gttacttaat
      301 gatttgaaaa tctaaataaa tacaacttgg cgagttggct gattgaagaa atagaaaatg
      361 agaggagaaa aataatggat gcaggaatta cagtaatgtt agataaacaa caaacgaatc
      421 aattgaaaaa ttatgttttt gaaatgacaa aagaggctat tgatcaagca agaattgata
      481 ccggattaga aagaccattt ctaaaaggaa aagagatggc taaatatttg aatgtatcat
      541 atacaacttt tttgaaattt aaaagaatgg gattgccagt tattttatta gaaaaaatgg
      601 agttgttttc aaaagaagaa tgcaaaa
//
LOCUS       DAYYHX010000401          617 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.409, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000401 DAYYHX010000000
VERSION     DAYYHX010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.409"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>617
                     /locus_tag="ACHA6Y_003012"
     CDS             <1..>617
                     /locus_tag="ACHA6Y_003012"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8989007.1"
                     /translation="LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYP
                     KTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRA
                     YQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSI
                     EIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTN"
BASE COUNT          261 a           53 c          106 g          197 t
ORIGIN      
        1 cattaaaaaa acattataca gagccttggg aagatgaagt ttttagagta attattgaat
       61 ataacaatgt tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg
      121 attatcatta tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc
      181 caaattattg gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga
      241 aaaaagaaag aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa
      301 taagggcata ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac
      361 acgagggcaa aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa
      421 atgttaaggc aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta
      481 ttgaaataat cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt
      541 ttaaaacaaa atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata
      601 attttttaaa tacaaat
//
LOCUS       DAYYHX010000402          614 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000402 DAYYHX010000000
VERSION     DAYYHX010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.410"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..436)
                     /locus_tag="ACHA6Y_003013"
     CDS             complement(<1..436)
                     /locus_tag="ACHA6Y_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8989008.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFE"
BASE COUNT          181 a          110 c           86 g          237 t
ORIGIN      
        1 tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat actttataag
       61 ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata tatttttcct
      121 gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga gataacagag
      181 ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg acggttactc
      241 gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg atcgtttttt
      301 tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac tctgataaat
      361 ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct ttgcttaaaa
      421 aaaactcata attcattagt catcagctct ttctacgtat attactttcc atgcattcac
      481 caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca gcgattcttg
      541 tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt tttatttggc
      601 ctttctcgcg atca
//
LOCUS       DAYYHX010000403          600 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.411, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000403 DAYYHX010000000
VERSION     DAYYHX010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.411"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            427..>600
                     /locus_tag="ACHA6Y_003014"
     CDS             427..>600
                     /locus_tag="ACHA6Y_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8989009.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQP"
BASE COUNT          223 a           95 c           86 g          196 t
ORIGIN      
        1 ttcgtttcta aatactccaa tttgggaatc caataaaaat tttcgttcaa atacgtgtct
       61 attcgaactc ttttctagtt gttaattatg aagggggaag aaataaaaaa tacaatctat
      121 acacgaataa gaatattatc caaaaggagg ttttagaaaa gatttattat acgtaaaaag
      181 ctcaagacat tttcttagcc ataaggacat gtttgatatt taagaagata tatgaatttt
      241 cttttctaag tataaagata actatccatt atcaactaaa aaaacatttt tgaaaataaa
      301 catcagcagt cctatcaatg catgtgagct tatagaagtt attcgcagcg cagcataatt
      361 gcattagctt cttttcgtgt tttttgcctc ttttcccttt aaatcttaat ggaggaggtg
      421 tgcaatatgt cagataacga cgaacgcgta aatgcccagt tgattcgatt ctttgaaaag
      481 gtcattttga acaccgctaa tacttattat aaaaaacaag aagaaattca aaaacatgaa
      541 caacttgatc aactcccacc atatttgttt actaaagata ctataaaatt taaacaaccg
//
LOCUS       DAYYHX010000404          597 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.412, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000404 DAYYHX010000000
VERSION     DAYYHX010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.412"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          204 a           74 c          137 g          182 t
ORIGIN      
        1 tttattatag aaaaaattta taaaaggagg tttcttgata tgataagaaa caaaaagggt
       61 ttgttgaaag ttgctttact ggcagctttt ttctaagtaa ctttttgaca agctcaatgg
      121 caacacaagc ggaagaagta atacaagaac taactgttat gaataacagc tagataaaat
      181 gggctatgta tgttatccaa caatttagta atcactagaa gcctagcgaa agttcgcttg
      241 ataagttata gatgagtatt gatttgtagt gtttccaaat caaaagggta cagagccaca
      301 tgaaatatgt tgtcctctgc agcgtcagga tgggaatcca taccatggag attcggagat
      361 ctgtgaggga atacttacgt ggttaatatg tcagttgtgt tggacgtagt aatggtcata
      421 gtgggtagat tgactgaatt cttatatgag aaaatgaaac caacaagaag atagggttat
      481 ttatacagaa ttggggatct tttctggaaa agtaatcgga taatgcgaga ttcgtacggt
      541 cttttttaaa tttctctaaa aaaaacggca tcgaatgggt tgggtgattg ataagca
//
LOCUS       DAYYHX010000405          590 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.413, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000405 DAYYHX010000000
VERSION     DAYYHX010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.413"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>590
                     /locus_tag="ACHA6Y_003015"
     CDS             <1..>590
                     /locus_tag="ACHA6Y_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM8989010.1"
                     /translation="YFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYA
                     LKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAW
                     VPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVV
                     PITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASA"
BASE COUNT          184 a           73 c          116 g          217 t
ORIGIN      
        1 tatattttga tgataaatat gttggcagag tagtcggcgg aacactgtca tttgtgttga
       61 taggtttagt aatttattta agaataatga taaagggtgc tcattttttt gataaaaaac
      121 agtggagata cgctttaaaa atctctttgc cgctaatccc acattcctta gcaaatatag
      181 ttttagcaca attcgataga attatgatta attcttattt aggtcctgct gcggggggaa
      241 tatattccta cattagtaac ataggtatta ttctaagcgt aatatggatg tctactaata
      301 acgcctgggt tccatggttc tatggtgaga tgagtaaaaa taatgaaaaa aaagtgaaga
      361 aagttagtaa ctattattta gtagtcttta ctttgattac gatgattgta atggtagttt
      421 cgattgattt agcaaaattt atggctccga aagactatta ttctgggtta tctttagtgg
      481 ttccgataac cttaggctat ttctttcagt ttttatatag tttaccagtt aatgctgaat
      541 tttatgagaa aaaaacatcg tttattgcga ttggtacagt agcaagtgct
//
LOCUS       DAYYHX010000406          573 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.415, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000406 DAYYHX010000000
VERSION     DAYYHX010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.415"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>573
                     /locus_tag="ACHA6Y_003016"
     CDS             <1..>573
                     /locus_tag="ACHA6Y_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8989011.1"
                     /translation="GYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFR
                     SVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMT
                     MVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYV
                     DILQKRAIYLGKEFLIGSIDSELLSFGIGLT"
BASE COUNT          181 a           80 c           90 g          222 t
ORIGIN      
        1 aggctataac ctttttcttt atattgttac gctcattact ctatttatat gtcattatgt
       61 aatgtatgaa attaccaaaa aacatgttac tagctccttt ttcgctatta tatatacaac
      121 atcttctttt aggagtgtag aaatatttct tcgaggggca atgggggaat tactcgcaat
      181 gtcaatactt cctcttattt tactcgggtt tataaagctt tacgactcaa aaaaagaatc
      241 ttgggttatg ttggcgatta gcatgacttt attaatttac actcatgttc tttctgtagc
      301 tatgactatg gtaatgatta taattgcttt aattattcaa ttttatcgga aaaagaaaat
      361 agaaatcttc ttaattatta aattaataaa agctgctata gttactttac ttctttcact
      421 agcgtttata ggtcctatga ttgagcagac attgtatcaa gatctgaata gaccttatgt
      481 ggatatatta caaaaaagag caatttatct gggtaaagag tttttgattg gaagtataga
      541 ttcagaatta ctatcatttg gtattggctt aac
//
LOCUS       DAYYHX010000407          568 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.416, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000407 DAYYHX010000000
VERSION     DAYYHX010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.416"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..>568
                     /locus_tag="ACHA6Y_003017"
     CDS             72..>568
                     /locus_tag="ACHA6Y_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM8989012.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          212 a          104 c           93 g          159 t
ORIGIN      
        1 atacaaaaaa ggaagtccct tctgtagaat aaagttaacg acaaccaatt cacagaaaag
       61 aggacttccc tatgaatgat tttactacag aaattgtgca aactctagtc actaaaggcg
      121 atttaaatga attattccgt tcgcacttag aaaaagcgat aaacacactc ctacggactg
      181 aattaacggc ttttttagat tacgaaaaat atgatcgcac tggttttaat tcaggtaatt
      241 cgagaaacgg ttcttacttt cgatcaatca aaaccgaata tggtgaatta acattggaaa
      301 tacctagaga tcgtaatggt gagtttaaac aacaaacttt accagcctac aaaagaacaa
      361 acgatacatt ggaaaccact attatccatt tattcgaaaa aggtgttacg atgtctgaaa
      421 ttgctgattt gatcgaaaaa atgtacggtc atcactatac tccacaaacc atgtccaaca
      481 tgactaaagt tctgactgaa gaagtaaatg cctttaaatc cagagcctta aatgataagt
      541 atgtcgctat ttttatggac gctactta
//
LOCUS       DAYYHX010000408          567 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.417, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000408 DAYYHX010000000
VERSION     DAYYHX010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.417"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(90..>567)
                     /locus_tag="ACHA6Y_003018"
     CDS             complement(90..>567)
                     /locus_tag="ACHA6Y_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8989013.1"
                     /translation="CIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIV
                     SVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAF
                     YYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
BASE COUNT          231 a          101 c           78 g          157 t
ORIGIN      
        1 gtttagccat atgtttcaaa aaatgaaaaa gaatgacagt ttttacataa aaactgtcat
       61 tcttaattga aagtttcaat ttataaagtc tacagatcca tagttctcat caatttacga
      121 ttgttgaagt aaatcaaacc aaatacgata gaaggaccta gtagaataca ccattggggc
      181 tttacgaaaa agaagagaaa caaacttgtc aatagtaaaa aatctagtaa actcacatac
      241 cattttttca acgtatagta gaacgctatc cgtagaactt gatttggttt cgacgctgga
      301 tttctcacct gaaaatacca gtagttgatg ctaaaggaaa ctgataaaca agcaagtaaa
      361 acaaacaacg gaatcaacca ttttccataa gaaaaacgta taaaaaagaa acaatcaaaa
      421 atcattatca cactaactat ccaagcaaat agccaataaa aaaatccctt tagccaaaat
      481 tggcgaaacg tctttatgaa caaacgtact ggtgaaaaac ttttttcttt tgctaattct
      541 tctgtcatac ctatcatcac gatgcaa
//
LOCUS       DAYYHX010000409          551 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.420, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000409 DAYYHX010000000
VERSION     DAYYHX010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.420"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>551
                     /locus_tag="ACHA6Y_003019"
     CDS             <1..>551
                     /locus_tag="ACHA6Y_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8989014.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSN"
BASE COUNT          203 a           87 c           95 g          166 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tattatattt ctttgttatg caaagaagaa
       61 gttcgcgaac tacctaaatc taattctgct gttggtattg acctaggtat tatagacttt
      121 gcaattcttt ctaccggtca aaaatttgat aataataaat ttacgtccaa aatggaaaag
      181 aaactcaaac gtgaacaacg taagttgtca agacgtgcct tattagctaa acaaaaaggt
      241 atcaatctgt ttgaagctag aaattatcaa acacaaaaac gtaaagtagc acgattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aattatcaaa
      361 aatcacgata tcatctgtat tgaagactta aacacaaaag gcatgttgcg taatcataaa
      421 ctagcaaaaa gtatttcgga tgtttcttgg tctagttttg tgtctaaatt acaatacaag
      481 gctgactggt acggtcggaa aatcatcaaa gtagataagt ggtttccatc tagccaaatc
      541 tgttcgaatt g
//
LOCUS       DAYYHX010000410          544 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.421, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000410 DAYYHX010000000
VERSION     DAYYHX010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.421"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>544
                     /locus_tag="ACHA6Y_003020"
     CDS             <1..>544
                     /locus_tag="ACHA6Y_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8989015.1"
                     /translation="FPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYI
                     FFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSI
                     PWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYD
                     IERLLKQKTMKNILKNVKIAE"
BASE COUNT          213 a           67 c          102 g          162 t
ORIGIN      
        1 tttcctaata tatatacagg aagagagaat acaatacaaa ttccattagt tacagctggg
       61 tcaagaaccg cattgcaact ttcggatatt tttaacccta tgatagatat ttataaagaa
      121 aaaaaatata tattttttac gtctgtttat gattttgagg gcggagaacc tgtcggagaa
      181 tatgaattag tatgcaaaat agcagactta gtaggaaaag ataatctaat tgttaagacg
      241 catcctagag atgtaagaac aatttataaa gataatggat ttatcgagga taaaaattca
      301 tcaatacctt gggaggcgat tcaattgtca ggcgatttta acgataaaat atttatgaca
      361 attaattcag gaagtgttat ttcaggtagt acaatgtctg caaaacctgt acgcacgtat
      421 tatatgtata aattgtgtaa catagaagga aatccgtctt gcaaaaagaa tgcgtatgat
      481 attgagcgat tgcttaagca aaaaacgatg aaaaacattt taaagaatgt gaaaatagca
      541 gaaa
//
LOCUS       DAYYHX010000411          541 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.422, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000411 DAYYHX010000000
VERSION     DAYYHX010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.422"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(174..539)
                     /locus_tag="ACHA6Y_003021"
     CDS             complement(174..539)
                     /locus_tag="ACHA6Y_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8989016.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          215 a           70 c          107 g          149 t
ORIGIN      
        1 tatctattat tggaatcatt attgctgcaa ttattacaca actgttttta gtgtttggag
       61 agaagaaaag aagtaaaaaa tagaaaaact agtgatagtt ttggtgatat aagaaaaaca
      121 tatatgtata aaaagaagaa agtagcttct ctttgtacat atatggtttg tttttatgga
      181 tgccgagctt ttttataaga taaggctacc gcccataacg acccaaggat ttgtggaact
      241 aagtaaatac ctgcaagaag caagatcagc tgggtcggtt ttaaaggatg atagaagtaa
      301 cgccattcag taaaattggc gcaaaataaa taggaagtga cagtagatat aatattggca
      361 atgaataaag atataatacg cctgttcctt tgaaaataat atccagcaaa aagagataaa
      421 aatatcaatg tgatgaaccc gcttacggaa tgataacgaa aatcaagcag taaggcgaaa
      481 tgcacataag gaacaaaaga taaaagcaac ggaaggtttt tccagcaagt ttttatcatc
      541 t
//
LOCUS       DAYYHX010000412          538 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.423, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000412 DAYYHX010000000
VERSION     DAYYHX010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.423"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..438)
                     /locus_tag="ACHA6Y_003022"
     CDS             complement(139..438)
                     /locus_tag="ACHA6Y_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8989017.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          174 a          101 c           64 g          199 t
ORIGIN      
        1 aggataaaat acaatataaa gaaacatctg tttctttatt tagaataaaa aaatacagac
       61 tctgttcaaa taaatgaacc tagcctgcat tatgagattg actatacttt ttaattcatt
      121 gagatgaata actcgttttt ataaatgccc aaaagtaatc ggtgccgaag ccattcctcg
      181 acctaattct ttttcaaatt ttcctattgt tgatatctcc atataaaacg gtgatactgg
      241 tttagacaat tctcccctga ttgctaacgg acgtaaacta cgttccaaat tatttatgac
      301 tttaggataa tactgacctt tttctaagcc tgtctttgcg tctatcaaaa gtatacgttc
      361 cttttctttg atctctggat ttaaaacgag atcatataat tttagtacca tgttttctat
      421 ttcttcactt ttagacatca cgatttcctc ccaaaaatat atttcttccc actacattct
      481 ataaacaaag tatacgctac atctagattt ttttaaatca ttactggatt gttaaaac
//
LOCUS       DAYYHX010000413          534 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.424, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000413 DAYYHX010000000
VERSION     DAYYHX010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.424"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACHA6Y_003023"
     CDS             <1..>534
                     /locus_tag="ACHA6Y_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8989018.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAYYHX010000414          528 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.425, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000414 DAYYHX010000000
VERSION     DAYYHX010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.425"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..459)
                     /locus_tag="ACHA6Y_003024"
     CDS             complement(<1..459)
                     /locus_tag="ACHA6Y_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8989019.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERS"
BASE COUNT          204 a          105 c           83 g          136 t
ORIGIN      
        1 agatcgttca ctttttatcc tgatttctgt ccagaagtaa aaaaatgttg caagaataaa
       61 tgaggcaatt actaaataga accatggttt gatcgtcaag aaatacatgg aggaataagc
      121 taaatccttc aatccactct cgctcaaacc attagggccg ataatcaagc gaggcagcaa
      181 agaaaacgta gatgtaaata ctaataagca agaaaagtaa agcagctgcc aattgtagtg
      241 attgatcaga gattttggta ataagagacc tacacttatc actaaataga aaacaatcaa
      301 agctggaaaa ttcctagaaa ccacaaatcc gcttccttgg atcaggccta atatctccgc
      361 accttgtaca acagcattta cccatgtaaa aggtagcaaa acgataggaa aaaaccaaaa
      421 aaataaataa agccgatcga ttactgcttt tcttctcatt aacgttcccc caaactactt
      481 tttctctcat catactatac aattttctat ctaaaaaaat taggggga
//
LOCUS       DAYYHX010000415          525 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.426, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000415 DAYYHX010000000
VERSION     DAYYHX010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.426"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..337
                     /locus_tag="ACHA6Y_003025"
     CDS             <1..337
                     /locus_tag="ACHA6Y_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8989020.1"
                     /translation="LISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEF
                     PDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDF
                     PEEIIDRIE"
     gene            complement(391..>525)
                     /locus_tag="ACHA6Y_003026"
     CDS             complement(391..>525)
                     /locus_tag="ACHA6Y_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8989021.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          180 a           81 c          106 g          158 t
ORIGIN      
        1 actgatttct gaaccagtaa agatcatcat cgatttttct tatggtgctg cctataatca
       61 atttatcaag aaaaatttaa gcttgtacat taatctgaac acggaagtga tagaggcagg
      121 aaagcctgag ttcccggatg ttataatcac gaataggaat aatctatatg aagaaagtgc
      181 atctcaagta gtggtttggc tcgatccgcc aagagcagta gactggggga atcttacaaa
      241 gattttgttg actatccaag aagagaaata caaagacagc aaaattgaca agcaaataga
      301 cgattttcct gaagaaatca tcgacaggat cgaatagtga acttctttta gaaagtccga
      361 ctgctttttc taaaaatatt atttgtcaaa ttaagcgaga caaaactttc catttacgta
      421 actcaaaaaa acttctaatg gtgtttggta atttagtgat tttctaggga tatggtttct
      481 tttggacgca acggatgaga tgaatccttg attgacttgg ttgaa
//
LOCUS       DAYYHX010000416          512 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.428, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000416 DAYYHX010000000
VERSION     DAYYHX010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.428"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..96)
                     /locus_tag="ACHA6Y_003027"
     CDS             complement(<1..96)
                     /locus_tag="ACHA6Y_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8989022.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGC"
     gene            complement(227..>512)
                     /locus_tag="ACHA6Y_003028"
     CDS             complement(227..>512)
                     /locus_tag="ACHA6Y_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8989023.1"
                     /translation="LVLFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLV
                     FNEEEIHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          184 a           87 c           69 g          172 t
ORIGIN      
        1 acaccccact tgaaatatat ttccctttcc taaaaaaata atgaccggaa aaaaaatatt
       61 taaagtaatc aaggttaacg gattgatttt tgacactctt tcacctccaa aaaataagat
      121 cgcactgtta tcttaagctg ataaaaatgc accgttattc agataacagt gcgatcttat
      181 caaaaagcat aactgagaac aattctcaat gcaagctttt ttttaattaa ctcagtaatt
      241 caatccaccc aggaacagaa aattgataca atcttctggt taatagcata gtcttttctt
      301 ttgaatagct atgcttcaat atatcataga atgtagcgta gaatgagtaa atgtagaagt
      361 ggatttcttc ttcattaaag actaaatttt gattgatcat tccgatagtc tttaacttat
      421 ctagacaaca gactgtcttt tttgtcacaa atgatgtaat tttttcaaca atatcttgat
      481 atttggttcc agctgattgg aataaaacca aa
//
LOCUS       DAYYHX010000417          509 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.429, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000417 DAYYHX010000000
VERSION     DAYYHX010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.429"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHA6Y_003029"
     CDS             complement(<1..132)
                     /locus_tag="ACHA6Y_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8989024.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DAYYHX010000418          502 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.430, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000418 DAYYHX010000000
VERSION     DAYYHX010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.430"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..411)
                     /locus_tag="ACHA6Y_003030"
     CDS             complement(<1..411)
                     /locus_tag="ACHA6Y_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8989025.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISY"
BASE COUNT          207 a           75 c           64 g          156 t
ORIGIN      
        1 ataactaatt acaaaactag caaaactttg taataacatc aaattaaata caagttggtt
       61 catatcaaaa aaagcatttg aaaacaaaaa atatatgttc cctattaata agaacacaat
      121 taacgaaagt gtagctagag aaagtaccga tgacataaat tcatttatgt catctttata
      181 gtcaaaactt gcattattaa ttgccgaatt taggcttagc ccaacaagaa tcgaaaaaat
      241 tgatacaaat gaagtgaaat tattaatcaa accatattct gcttgagtag taatatttac
      301 aaaaatagga atagttatga atgaaattcc cttaataaaa aaattcccta tagtatacca
      361 gaaactggac ttaactaatt ttgacttgga aagtgtgtta aataatccca tactctacca
      421 actttcttaa ttctgcccag ttttctggga gtaaaatttt ttcttctgat agacggttat
      481 aatgttcaca gaaaagaaca at
//
LOCUS       DAYYHX010000419          494 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.432, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000419 DAYYHX010000000
VERSION     DAYYHX010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.432"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          167 a           96 c           57 g          174 t
ORIGIN      
        1 aaaataagaa tgctaaatca ctctacaata acggcgaaag cacaaaaaat aaaaaaatta
       61 ttacgttatt aaaaaaacag atgaaagatc aggttttatt agatctcatt ctttcatcac
      121 ttgaagggat ttaatgaact actttttcct ctttgtcgtt tttatatatt cgtattcctt
      181 caaacagtag gatagcctct ttcaatgctt gatatttgaa ctgtccgtta cttaaaaatt
      241 tagatgtgcc gccctcttcc tagaacatat tcttttttac tttttcatta attgacagac
      301 caatcgaacg aaactgtaaa tttcgatagg ataatgattg gtcaaactca ttcattttca
      361 agacatctcg attcatattc aagataggaa tctttttatc aacatcactt tattcgtatt
      421 ttcctctacc ataaaagctt cacgctccac aaaattgttt tatacactac tattcagccc
      481 attcaacgac caat
//
LOCUS       DAYYHX010000420          493 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.433, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000420 DAYYHX010000000
VERSION     DAYYHX010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.433"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..>493)
                     /locus_tag="ACHA6Y_003031"
     CDS             complement(54..>493)
                     /locus_tag="ACHA6Y_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8989026.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT          133 a           92 c          115 g          153 t
ORIGIN      
        1 tcgtaaaaaa ttttcgatct gggcaaagaa gtggtatact gtaagtggtt tagttaatgg
       61 tccactgaga ccgcgtacgc atagtcgtac tctgtatgtg ccctaaccat agcgtgcata
      121 catttcagaa gcttgttcat acaagcaact gtggcaacct tatctttctt agggataggt
      181 tgctttttta atttgtagta ataatcgaca atatgattag gagctgcgcg ttgctggcga
      241 atcatatttc gaatggtgaa gaaaataatt ttccgtgctt tgggattacc acgcttattg
      301 atgcggtctt ggccagtata cttacctgaa tgataccgtc ggatgtcgat tccgacaaag
      361 gcattcaatt gtttatgggt tttaaatcgt gtgatatctc ctaattcacc gagcaataaa
      421 gcggctgtga gtgcgccaat cccagggaac gtttggtaca acaaaaagca aggagagcct
      481 tcggcttttt tga
//
LOCUS       DAYYHX010000421          479 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.435, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000421 DAYYHX010000000
VERSION     DAYYHX010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.435"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACHA6Y_003032"
     CDS             complement(<1..430)
                     /locus_tag="ACHA6Y_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8989027.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          135 a           95 c           64 g          185 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tctaactttt ggggtgcac
//
LOCUS       DAYYHX010000422          477 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.436, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000422 DAYYHX010000000
VERSION     DAYYHX010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.436"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          165 a           87 c           58 g          167 t
ORIGIN      
        1 ttaggattta tggtactttt tattcggcaa tccattttat ttttctactt cttcttaata
       61 cctctaacac ttttgattta taaactaatc caatgcatct acttgcgctt attaagctat
      121 ttcagctaga tagtaccacc ccatgcgtca atgcttgatt ttccttttgt ataacattgt
      181 ataagcaata aaggacaaat aagtggtgat tacaccaaac cattgaataa gaaaaattct
      241 gatacgtaaa taaacatgaa cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg
      301 tcttttttct catacagttc tgttaactta cacttctctt cgctagataa taaaaaatcc
      361 tccaactgta ttgtccaata taatcccagg catctaaact agcattatag aagagacaca
      421 catagaatct aaaataactg gttttaaatg tttcttacaa tcaaaaaaag ctgttaa
//
LOCUS       DAYYHX010000423          470 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.437, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000423 DAYYHX010000000
VERSION     DAYYHX010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.437"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>470
                     /locus_tag="ACHA6Y_003033"
     CDS             <1..>470
                     /locus_tag="ACHA6Y_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HFM8989028.1"
                     /translation="ALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKT
                     TNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLV
                     KKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAV"
BASE COUNT          167 a           78 c           88 g          137 t
ORIGIN      
        1 aagccttatc aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa
       61 atagaggatt aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca
      121 atgaagccaa aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag
      181 cgatcgtaac agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca
      241 ttttggatct gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg
      301 ttctagtaaa aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc
      361 atactgatcg tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg
      421 acatcaatcg atcattgagt cataaaggct gtccttttga taatgccgta
//
LOCUS       DAYYHX010000424          464 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.438, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000424 DAYYHX010000000
VERSION     DAYYHX010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.438"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            84..278
                     /locus_tag="ACHA6Y_003034"
     CDS             84..278
                     /locus_tag="ACHA6Y_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8989029.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          201 a           60 c           81 g          122 t
ORIGIN      
        1 atcataaaga tatagataca taatcatttt cacttacata tatttgaata ttaactaaaa
       61 acagcaagaa agaagaggat caaatgtatt atgtagaagt aaaaacaaaa ggcgtcaaaa
      121 acaaacaata tgtgaaaggt atcagtaacg aatatccttt acttggttca tggaaagaag
      181 ctgctccttt ttccaaacca tgtgccataa aaataaagaa cgagctagaa aaagaactga
      241 cttgtggtaa agctgttgta gatatcatcg aaaagtaaat ccaatgtata gaaaggaaga
      301 tcacacatga aaagcgacgt acaaattgct aagattatag aattagaaga agaagacgca
      361 gtgttgacaa ttgaaaaaaa ataaatgttt gtataccata tgattatttc aatatgtatc
      421 ctggaattgg agatctcgta gagctatatc aagatgaatc aaat
//
LOCUS       DAYYHX010000425          463 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.439, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000425 DAYYHX010000000
VERSION     DAYYHX010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.439"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>463
                     /gene="scm"
                     /locus_tag="ACHA6Y_003035"
     CDS             <1..>463
                     /gene="scm"
                     /locus_tag="ACHA6Y_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8989030.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTS
                     DSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNF
                     NLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSK"
BASE COUNT          172 a          107 c           77 g          107 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gtgacagtac agatactacc tctaccacaa gtgatagctc agattcatct
      121 accactacaa gtgatagcac ggacacgtca gcttcaagtg acagtacaga tacatcctct
      181 accacaagtg atagctcaga ttcatcaact tcaagcgata gtacagacac gtcgtctacc
      241 acaagcgata cacgtcatgc aagtgtctcc ttctcaaaaa caagttcgga taacaggacg
      301 aactttaact taactaataa aacaagcaat gacaatggaa acaaatcaaa aagagcactt
      361 cctaaaactg gaagtcaatc gaataattgg ataacactcg caggcgttat attacttgta
      421 atcggtttaa gaatcacttt tatgagttat aagaaatcga aaa
//
LOCUS       DAYYHX010000426          457 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.441, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000426 DAYYHX010000000
VERSION     DAYYHX010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.441"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..>457)
                     /locus_tag="ACHA6Y_003036"
     CDS             complement(86..>457)
                     /locus_tag="ACHA6Y_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFM8989031.1"
                     /translation="DAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIV
                     PQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKAD
                     YVTVSNDKDGVSQAFERILTI"
BASE COUNT          150 a           78 c           61 g          168 t
ORIGIN      
        1 acactgaaga agtactattt gtactggaat ttatgctcca tttttccgta tttagaattc
       61 tatgttctat actaccttaa ttagtttata tggtaagaat tctttcaaat gcttgagata
      121 ctccgtcttt atcatttgaa acagtgacat aatcagcttt tttcatgatt tcttgatgac
      181 tatttcccat tgcaaaacta atatctgcac tttcaaacat agataagtca tttaaactat
      241 ctccgatcgc gattctcaaa ggtttcttta aattgtagat tgattccaaa cgatcaaaca
      301 ccacaccttt agatacattt tgaggtacga tttcaatatt tacaggtgcc gattcagtaa
      361 cagacacatt tagcatcttt ttcaaatggt taaatatatt agctttcaat tgacgattag
      421 gaataaaaat aaaaaatttg taaatgctga tagcatc
//
LOCUS       DAYYHX010000427          448 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.443, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000427 DAYYHX010000000
VERSION     DAYYHX010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.443"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..305
                     /locus_tag="ACHA6Y_003037"
     CDS             141..305
                     /locus_tag="ACHA6Y_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8989032.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
BASE COUNT          157 a           60 c           93 g          138 t
ORIGIN      
        1 aaaaggaata tttctatgag ataacgaaaa tggcctgtat aataaagaag aggcctagaa
       61 gaacatcata caataagata gctttcaaaa tactcattga aagaaggaaa tactataacg
      121 taagaaagga gatgaataaa atgaaaaacc tgattgctgg cggctttttg tttgtgggtg
      181 gtggcatact tatgaataca gataactttg ggaatgtcat cccttttctt ggtgcagctt
      241 gtgctttgat tggtgtagga atgctgattt atttcgtatt tagcgatgat gggaagtatg
      301 gttgattatg agtagccaac tctactcttt cgtaaagatt acaacatacg tgacaaaaac
      361 agaaagcttg attcatcata ataataaaag aaaaagaaac ccaagtgatt ggatttcttt
      421 ttctttgcat aagctttatt tactccgg
//
LOCUS       DAYYHX010000428          429 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.444, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000428 DAYYHX010000000
VERSION     DAYYHX010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.444"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          132 a           72 c          101 g          124 t
ORIGIN      
        1 taccgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgaaaa agtcgcttag
       61 tttcgagtag taagaaaaat tttgtgaaaa tggctttcta catttttgca taaatttggc
      121 ttaataccga gaaactggct tttgaacacc gtttaccaag aggttgtgag cctgccgttt
      181 aactccgagt cacaaggagc aatttcaaaa aacagcttct caggtttgtt gactattgtg
      241 acttgtgacc gaggagttgg caggagaaca ccgtttaagg aagaggttgt aaaatcagcg
      301 gtttgacctg agtattagag atacaaaacg gaaaaatagc ttctcatatt ttgccgtttt
      361 ttgagctaat accgaaggac agctgattga acaccgttta ttcggaaaaa gggactgtga
      421 cacgacttt
//
LOCUS       DAYYHX010000429          419 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.445, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000429 DAYYHX010000000
VERSION     DAYYHX010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.445"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          121 a           75 c           55 g          168 t
ORIGIN      
        1 tcttcacatt tattaggtta tagtttaaga aagtttaaac attgcgcaat ttaattattt
       61 tagctgtttc ttttcataaa aaaacatgtt gtgactctga gacaaatcat agtccatagt
      121 gatgtctcta gagtaactac gcctaatatt acgaggcatc atattaatct atcagcacta
      181 gtacctaaac tagcatccct ttcgaatatt ttttcttata taataaagtt tgctttcact
      241 cctctaatct caccaaatcg actggcttgc gttgtgtgat actaggaact gattggttta
      301 gaacgacttt tctattgtaa tcatattttt cgccatcctc gttgtcttct aatccttcaa
      361 tgaatgatcc ctaatgtgtg tattaaataa tttaattttt ttgttcctat ttatttaac
//
LOCUS       DAYYHX010000430          414 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.446, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000430 DAYYHX010000000
VERSION     DAYYHX010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.446"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          152 a           53 c           58 g          151 t
ORIGIN      
        1 aataaagtac tacctttaat aggtagtact ttattatcaa gccattattc cttcttctac
       61 tagttctttt tgtcacataa ttttgcaatc aataaatgtt gcgcaataag tataagaatt
      121 ccattttcta aatttgaaag gtgatccata atttcatgtt caagtagttt attaattgtc
      181 cacttgtcgc ttcatctaaa aattttatag gttttaggaa tgctattcgc ttattttagg
      241 aagattttaa ctgtttgctg tagatatata tcatactagt atcgtaataa aagtactcta
      301 aaagaagaat tttcaaatac gcaaaattaa gtacgaaaga gttgaaacaa atttggagaa
      361 aaatgagcat agaatgtcta atgagttaaa tagacctaaa aattatctaa aagt
//
LOCUS       DAYYHX010000431          406 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.448, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000431 DAYYHX010000000
VERSION     DAYYHX010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.448"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          145 a           50 c           70 g          141 t
ORIGIN      
        1 ataacgtgat tttttgctgt aatgacaagg ttttagctgt ttcagatacc atgtatccaa
       61 aaatagttta ttgagaaaat aggagagtat ttgatactaa ctagtaggaa aaaatgagat
      121 aaaaagtaaa tggaatcatt taaggtaatc tgaaataaaa aatatacagc cctgtggtac
      181 tttacctaca aggctgtata tattttttac tctcagcgtt tttgactccg tgtctgactc
      241 cgttttattg attttgaatg ttattcaatg accatttaac aggtcaaatt tgatgttttt
      301 gattccgtat gacgccgttt gatatctagt gatatagtga tttgagtcta catcgaataa
      361 gggaataaac caataaaaaa tgatttagaa tattaaaata acaaaa
//
LOCUS       DAYYHX010000432          405 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.449, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000432 DAYYHX010000000
VERSION     DAYYHX010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.449"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..394)
                     /locus_tag="ACHA6Y_003038"
     CDS             complement(<1..394)
                     /locus_tag="ACHA6Y_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8989033.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLND"
BASE COUNT          138 a           74 c           73 g          120 t
ORIGIN      
        1 gatcattaag atcgaagtat acaaaattat tctgtagttt ataaaaaaac ggcgaatgaa
       61 gatgtgtttt catatgacca aaatataata attctgaaat ttctaaaggt gtcaattctt
      121 tcagcatcgt taaatcagta aagtcgatcc acttcaattc atcgaatcca cgcctgttac
      181 gagaagagga aaagaaacgc tgaatatttt ctgtaccacg gattactttc agccctgtat
      241 gggattcata ttctccagca gaagcagatg gatcaagcaa gagcaagttt tgaggaaaat
      301 gcacgatact gtttgcaaag tcagtagcag tcagcccttt tgttaaaaca gcattacttg
      361 tggtatcaat atgtacataa actgactcag tcatcatgtt cctcc
//
LOCUS       DAYYHX010000433          404 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.450, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000433 DAYYHX010000000
VERSION     DAYYHX010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.450"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>404)
                     /locus_tag="ACHA6Y_003039"
     CDS             complement(<1..>404)
                     /locus_tag="ACHA6Y_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8989034.1"
                     /translation="DKIVMGKRIREVRKNREETLEKFGKNFTIYTGKNVISRWEKGIN
                     IPDIEKLMNIAYLGKVTVPYLMYGEDYKNILPKDERVSDFKKINSFSMGLRMRKIRKD
                     YYLEREEFGKLFSPSISKWSIDRYENGRDIPN"
BASE COUNT          115 a           85 c           44 g          160 t
ORIGIN      
        1 attgggtata tctctaccat tctcatacct atctatggac cattttgaaa ttgaaggaga
       61 aaaaagcttt ccaaactcct ccctttctag ataataatct ttcctaattt tcctcatcct
      121 taatcccata ctaaaagaat ttatcttttt aaaatcgcta actctttcat cttttggaag
      181 tatattctta taatcttctc cgtacataag gtaaggaaca gtgaccttac caagataggc
      241 aatattcatt agtttttcaa tatctggaat attaatacct ttttcccatc tacttataac
      301 atttttgcct gtatagatgg taaagttttt accaaatttt tcaagcgtct cttcccggtt
      361 ctttcggact tctcgtattc tttttcccat aacaatttta tcgt
//
LOCUS       DAYYHX010000434          400 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.451, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000434 DAYYHX010000000
VERSION     DAYYHX010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.451"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..331
                     /locus_tag="ACHA6Y_003040"
     CDS             140..331
                     /locus_tag="ACHA6Y_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8989035.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          156 a           59 c           65 g          120 t
ORIGIN      
        1 aaacatcgac tttacaaaaa aattcaaaaa atgctggttt aatatctgca taataactaa
       61 ttttgtatgc ttctttgctc gtcctcctcc ttccccaaaa aataaataga ttataagttc
      121 atgggaagaa ggtaaattta tgaatacttt aaaagaagat ttcattttag caaagaatgg
      181 aaatgaagaa gcagttgtgg ccattctcaa aagatttagc gctttggtcc ataagcaatc
      241 ctggagaact ggaaaatatg atcaagattg ttatcaagaa tgtatgcttg caatatatct
      301 agctatctcg aaattcgaaa ttaaagaata actcaactaa gggagtctgt gacataagta
      361 aaaagatagt ttcaaatttc gaacaataaa tttgaagcca
//
LOCUS       DAYYHX010000435          386 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.455, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000435 DAYYHX010000000
VERSION     DAYYHX010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.455"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..>386
                     /locus_tag="ACHA6Y_003041"
     CDS             11..>386
                     /locus_tag="ACHA6Y_003041"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8989036.1"
                     /translation="MSQTLKEIEIVLVDDGSPDASGEIADEYQKKYSNVKTIHRENGG
                     LGPARNTGIENATGEYVAFLDSDDWVESDMYEKLYLVASKENADIVVSGHCDFANDKA
                     MVKKQHPLSGNVYDTPNSIKNVR"
BASE COUNT          145 a           39 c           96 g          106 t
ORIGIN      
        1 ggatagttta ttgagtcaga cattaaaaga gatagaaatc gtattagttg atgatggttc
       61 gccagatgct tcgggggaaa tagcagatga gtatcaaaaa aaatattcta atgtaaaaac
      121 aatacataga gaaaatgggg ggttaggacc ggcaagaaat acaggaattg agaatgcgac
      181 aggagaatat gtggcttttc ttgattcaga tgattgggta gaaagtgata tgtatgaaaa
      241 attatactta gttgcgtcca aagaaaatgc tgatatagta gttagtggac attgtgattt
      301 tgcgaatgac aaagctatgg tgaaaaagca acatcctttg agtgggaatg tatatgatac
      361 gcctaatagt attaaaaatg tacgaa
//
LOCUS       DAYYHX010000436          385 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.456, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000436 DAYYHX010000000
VERSION     DAYYHX010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.456"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..>385
                     /locus_tag="ACHA6Y_003042"
     CDS             12..>385
                     /locus_tag="ACHA6Y_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8989037.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGS"
BASE COUNT          130 a           52 c           95 g          108 t
ORIGIN      
        1 gggacggttt aatggctgat attgtgcaat taaaagaaga cggagttgct aaatacttaa
       61 aaacgcatgt agaagctatt gatggtaaag aagcattggt gcagacagat ggagaccaag
      121 ccattgcagg acataagaat ttttcaggtt ctgtaacaat caataataaa cgtgttttga
      181 cgactgatga taacagatat gaagtggtaa atctggttgt cactaacggt aatacaggga
      241 cagcaaagct ttatcgtgaa ggaaaaacag tttctatata ttttgtggct ttaaacggaa
      301 aaagtagtgg cgggaatgat tcggttattt taactgttcc agaaggctat cggacaccaa
      361 ttagttttga acaactggtt gggtc
//
LOCUS       DAYYHX010000437          378 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.460, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000437 DAYYHX010000000
VERSION     DAYYHX010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.460"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..>378)
                     /locus_tag="ACHA6Y_003043"
     CDS             complement(148..>378)
                     /locus_tag="ACHA6Y_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8989038.1"
                     /translation="EKYQEYLLSLRIYVPSESLSELPLKEIKHRLSDVTFKRIKKKRR
                     SIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT          125 a           68 c           63 g          122 t
ORIGIN      
        1 taataataaa gtcccatccc gccaaacatg accaaaaaac cgatgactga tcgaatagga
       61 aaattaggaa caaaaaagag acagacggat aataatgatg ccggaaccat tattcgataa
      121 tagagttgtt tatccatttt cttcacctca tattttttgt aaagtaatca attgttctaa
      181 ttctcggtaa tctaaaaatt gcacacgcag atgctgctca cctttcttga catcgaattg
      241 gatacttcgc cgctttttct ttattcgttt gaaggtaaca tcagagagtc tatgtttgat
      301 ttcctttaac ggtaattctg acagggattc tgaaggaaca tagattctta aggataaaag
      361 atactcttga tatttttc
//
LOCUS       DAYYHX010000438          376 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.461, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000438 DAYYHX010000000
VERSION     DAYYHX010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.461"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..305
                     /locus_tag="ACHA6Y_003044"
     CDS             <1..305
                     /locus_tag="ACHA6Y_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8989039.1"
                     /translation="STPSGDKIQKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIAL
                     QAEKLLEGLNSDAETNYYGEPLDDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
BASE COUNT          153 a           50 c           63 g          110 t
ORIGIN      
        1 aaagtactcc atctggagat aaaatacaaa aagttgcaga ttactttaat gtatcaactg
       61 attatctttt aggacgtact gataaacgcc attattatga tttaacagaa aaagataaac
      121 aagatattgc tttacaagct gaaaaattac ttgaaggctt gaattcagat gctgaaacaa
      181 attattatgg tgaaccttta gacgatgaag ataaggaaaa attatacgat gccattcaac
      241 ttgcacttgc cttaaccaaa gttaaagcaa agaaaaaatt cactcctaaa aaatatcgtc
      301 aataggtggg ctttggtatg catttaaaca aatactggat attagaagaa atagaaaaat
      361 tatttgtaga gtttgg
//
LOCUS       DAYYHX010000439          374 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.462, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000439 DAYYHX010000000
VERSION     DAYYHX010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.462"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          128 a           56 c           67 g          123 t
ORIGIN      
        1 gtatgtctta gtccatcaat aaacagtcga agagaaaata gggaaaaatt gattactaaa
       61 gaaaaactta ctaggtcgac ggtgtagttg attttcatac ttcaatagat tcgaattaaa
      121 cgatttcgat tggcttatac cagtcctatc caacgaaaga tatatcgttc ttaactctag
      181 cggtataact tgaagcttta cgtaaagcca atgggaatga actggagatg actttttatt
      241 atttttagta atagctgatg ggttaatact tacaactttc cttttcaaag taccatataa
      301 aaaaacggtg gggttgttta gcgaaataaa ggatctaagc caatagctta gatcctttat
      361 tctatgcttt atat
//
LOCUS       DAYYHX010000440          367 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.465, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000440 DAYYHX010000000
VERSION     DAYYHX010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.465"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>367
                     /locus_tag="ACHA6Y_003045"
     CDS             <1..>367
                     /locus_tag="ACHA6Y_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM8989040.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKGER"
BASE COUNT          137 a           61 c           80 g           89 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
      361 gaacgag
//
LOCUS       DAYYHX010000441          366 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.467, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000441 DAYYHX010000000
VERSION     DAYYHX010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.467"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            67..>366
                     /locus_tag="ACHA6Y_003046"
     CDS             67..>366
                     /locus_tag="ACHA6Y_003046"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8989041.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPT"
BASE COUNT          137 a           45 c           70 g          114 t
ORIGIN      
        1 gtatatgttt tgtgagaaat ttagacttac aataagcagt gaatttataa tcaacgtata
       61 ggagacatgg atgaaatgaa actaattggt atagatttag acggtactct tttgaattct
      121 gaccagagca ttagtcaaaa aaatgcaaga acaatgaaac atttaccgga agactgtttt
      181 ccattcattt gttctggaag agaagtagaa gacattaata atattctcaa aaaatctggt
      241 ttaaggatac cagcagtagg gttaaatggt tctgttggat ttgatggaga aaaaaagata
      301 tttgagtctt ccttcgataa aaatgatgta aaaaaaattg attctgtcct atcgaaattt
      361 ccaacc
//
LOCUS       DAYYHX010000442          346 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.470, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000442 DAYYHX010000000
VERSION     DAYYHX010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.470"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>346
                     /locus_tag="ACHA6Y_003047"
     CDS             <1..>346
                     /locus_tag="ACHA6Y_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM8989042.1"
                     /translation="YAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNS
                     EISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGN
                     MHNGFLQVLVSSG"
BASE COUNT          126 a           47 c           68 g          105 t
ORIGIN      
        1 attatgctaa ggatttttta gcaacaattc ctgtatcgat tgatagaaaa gtagaagtta
       61 tagagtcaaa tgaatcagat aaaactccac ttccagaaaa agaagtaata ttacagcatt
      121 ctagtgataa ctccgaaatt tcagctggtc gttttacgat ttggaaagct gcgcttaaag
      181 ttataagacc aactttgtta tttggttatg gagagaccga tttctatggg aatagcgatg
      241 caaaattttt gaaaaaagta gatcttgata ctacagatgt gaatgaacta aaaagagcac
      301 atggaaacat gcataatgga tttcttcaag tacttgtatc aagtgg
//
LOCUS       DAYYHX010000443          346 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.471, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000443 DAYYHX010000000
VERSION     DAYYHX010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.471"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..259
                     /locus_tag="ACHA6Y_003048"
     tRNA            186..259
                     /locus_tag="ACHA6Y_003048"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:220..222,aa:Ile,seq:cat)
BASE COUNT          102 a           72 c           61 g          111 t
ORIGIN      
        1 caaaataaaa aaacgttgat ttaacaacgt ttttcataca tgtttatctg tctcaaccat
       61 acatatatgg agacgcatat cttcactatc actcataaat agtcacactc caagatatgc
      121 gataacaggt ttgttgccac gcattctact ggttgattgt ttatggcttt atgtggcaac
      181 aaccactacc cttagctcag ttggtcagag cagacggctc ataaccgtcc ggtcgtaggt
      241 tcgagtccta cagggtacat taatgtagcc aattgaatcg ttctgtgtta gaattttttg
      301 aagagtatta tacaagctaa agcttttctt cattgccact caaatg
//
LOCUS       DAYYHX010000444          337 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.473, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000444 DAYYHX010000000
VERSION     DAYYHX010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.473"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          126 a           55 c           59 g           97 t
ORIGIN      
        1 cttaagatta atgacactaa attaacagac tatacgtagt atctatttgc tatgaccata
       61 tcttatatcc ataagtcaac tcagccggac taaaagggct aatcttacta gaacacaagc
      121 tgcacttgat atgcaaatta accctgaatc ggaaaggtgc ttaaattgga tattaacgga
      181 gggataaagt aaaatttatc tctagggaat gtttagaaag gatccaagtt ccttaacaag
      241 tgacaattag taatgtagta acaacgatag gttgtatgcc gaacatttca atttgatacg
      301 tataaaagac gatttcaagc tcatccgtga taaataa
//
LOCUS       DAYYHX010000445          336 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.474, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000445 DAYYHX010000000
VERSION     DAYYHX010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.474"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..319
                     /locus_tag="ACHA6Y_003049"
     CDS             74..319
                     /locus_tag="ACHA6Y_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM8989043.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT          108 a           55 c           69 g          104 t
ORIGIN      
        1 catttctttt tttttatggt aaacttgaaa attttaaaaa tatgtggtat agttgatagt
       61 agaggtgaac tgtatgccaa caactttagc ttcaatcaag aaagatttag aaggtcgtat
      121 cggcagccca atcatgttgg ttgctcagac aggaagaaag cgtcaaacag aacgccgcgg
      181 cgttttaaca gaaacctatc cttcagtatt cgttgttgat ttggatcctg aagaaaattc
      241 ttttgaacga gtatcttaca gctattcaga tgtacttact cggacagttg agatcgaatt
      301 tttaactgaa gcagtttaaa aagcaaacga gcctga
//
LOCUS       DAYYHX010000446          330 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.476, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000446 DAYYHX010000000
VERSION     DAYYHX010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.476"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..>330
                     /locus_tag="ACHA6Y_003050"
     CDS             64..>330
                     /locus_tag="ACHA6Y_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8989044.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLH"
BASE COUNT          122 a           45 c           65 g           98 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat
//
LOCUS       DAYYHX010000447          326 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.477, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000447 DAYYHX010000000
VERSION     DAYYHX010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.477"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(94..>326)
                     /locus_tag="ACHA6Y_003051"
     CDS             complement(94..>326)
                     /locus_tag="ACHA6Y_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8989045.1"
                     /translation="RESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFS
                     DSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          117 a           57 c           38 g          114 t
ORIGIN      
        1 agaaatttcc caaaaaattt tttgttaaaa tccaatttcc tcaactcctc caaactacgt
       61 aaatgatata gtctcataat aaaataaaac ctctcagcta atgttgttga aattatccac
      121 taaacctaat tgatattgct taggagatac acctgcgtac tttttgaata ctctcgaaaa
      181 gtgtgaagaa tctgaaaatc ctaaacctaa agaaacttca ctgatagtat ggtttgattt
      241 caacattatt ttagccgtag aaatttttcg cttagagata taattataaa tcgtaatatg
      301 catttcattc ttaaagcttt ctctta
//
LOCUS       DAYYHX010000448          321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.479, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000448 DAYYHX010000000
VERSION     DAYYHX010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.479"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACHA6Y_003052"
     CDS             complement(<1..>321)
                     /locus_tag="ACHA6Y_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8989046.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAYYHX010000449          320 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.480, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000449 DAYYHX010000000
VERSION     DAYYHX010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.480"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          129 a           33 c           71 g           87 t
ORIGIN      
        1 actttttaaa aaaagtgttc caataggctg tgcctattac gtttctcaaa aataattgtg
       61 ggaacaaaat atttaaaaat aggatagtga aaggatagca aaggatagta aaaggataga
      121 agcaggatag cgaaaaggat aggctccttt tcgctatcct ttttaaattg gaggaattaa
      181 tatggaagta agaataagaa atgtagatga gaaaatcgta agagaaattg atcgaagatg
      241 tgacgaacta agtaaccgaa cgggtaataa atggagtcgt aacgattatt taaagctact
      301 cgttgaaaat gattttgatc
//
LOCUS       DAYYHX010000450          317 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.483, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000450 DAYYHX010000000
VERSION     DAYYHX010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.483"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>317
                     /locus_tag="ACHA6Y_003053"
     CDS             <1..>317
                     /locus_tag="ACHA6Y_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8989047.1"
                     /translation="EYTDYCYRKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLE
                     CQIRAKRMAIDYCRLYVGLVDDANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQ
                     QKF"
BASE COUNT          135 a           32 c           58 g           92 t
ORIGIN      
        1 agaatataca gattattgtt atagaaaaga agaacaagag tctattacaa aaagtttatc
       61 tccaaataag cagacggaat ttaaagaatt tttaataact ctaaaagcta tagcatcgaa
      121 agaagattta gaatgtcaaa tcagagcaaa acgtatggca attgattatt gtaggcttta
      181 tgtgggctta gttgatgatg caaatttggg aattaaagag aaaagaaagt atataaaaaa
      241 atttattgaa gatgagggat taattcaatg ttggaaggga tatcctacaa atacgctacc
      301 atttcaacaa aaatttt
//
LOCUS       DAYYHX010000451          294 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.486, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000451 DAYYHX010000000
VERSION     DAYYHX010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.486"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>294
                     /locus_tag="ACHA6Y_003054"
     CDS             <1..>294
                     /locus_tag="ACHA6Y_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8989048.1"
                     /translation="NKTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGI
                     CTAAFRANNLSCMIYGIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLP"
BASE COUNT          111 a           42 c           50 g           91 t
ORIGIN      
        1 ggaataaaac attaggacaa acgatacaca ataaaagatg ttatgtgata ttaattaatc
       61 aaaacctatc accttctatg aaaagatttg tattatggca tgaaatagcc cacgttagac
      121 tccataaagg tatttgcaca gcagctttta gagcaaacaa ccttagttgt atgatttatg
      181 gtatagaagc tgaagctaat atgtttgcaa ttgaaatgtt aactagaaca atagatgaac
      241 aagatttatc tgatatgagt cagttccaaa ttattgaata tttagggctt cctc
//
LOCUS       DAYYHX010000452          292 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.487, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000452 DAYYHX010000000
VERSION     DAYYHX010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.487"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..185
                     /locus_tag="ACHA6Y_003055"
     CDS             <1..185
                     /locus_tag="ACHA6Y_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8989049.1"
                     /translation="YTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQ
                     RILDNELGCANASEAV"
BASE COUNT           83 a           63 c           69 g           77 t
ORIGIN      
        1 aatacacatt tgaaacctta caacaattgg atttggagtt atttgactat gtcaattggt
       61 ggaaccacct tcggttgcac ggtacacttg gctacgagac accggttggt taccgtaacc
      121 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
      181 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      241 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tc
//
LOCUS       DAYYHX010000453          291 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.488, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000453 DAYYHX010000000
VERSION     DAYYHX010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.488"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>291
                     /locus_tag="ACHA6Y_003056"
     CDS             <1..>291
                     /locus_tag="ACHA6Y_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8989050.1"
                     /translation="FGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKE
                     TLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSM"
BASE COUNT           95 a           42 c           46 g          108 t
ORIGIN      
        1 tttgggatag aagaagtttt tgtcggagat aaatgtatct tttttgatca acctttatta
       61 tctgatggtt atggcataga tgaaacagat attttcgcta aagtaaatag tatattttct
      121 ggaaaaaaag aaactttaat taagcttcat cctagagcaa ggacaaatcg ttatggtgaa
      181 aaaaacatat tgaattccag tttaccgttt gaagtcttgc tacttgaaaa tgacttctct
      241 tcaacacttt tgattagtcc tatatctaca atttcttttt caccatctat g
//
LOCUS       DAYYHX010000454          290 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.489, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000454 DAYYHX010000000
VERSION     DAYYHX010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.489"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          102 a           37 c           52 g           99 t
ORIGIN      
        1 attagtaaaa atagaattag aagaattaac aagtttgatc cttctggaaa tatgaaggca
       61 cctggaaatg tcctcaatga agtagatgaa agtttaaaaa aatattttac aaagtaaacg
      121 atatttttgt tgacattttt ctgttcgtta gttaccatgt agttatagaa ggacgtaagc
      181 gcctattttt aatgtacaca tcgggacgta tgcgcccaca tagaaacata gttgagagac
      241 atctatatgg tgtctctttt tttattaata tgcaaaaaag tctagttttt
//
LOCUS       DAYYHX010000455          281 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.490, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000455 DAYYHX010000000
VERSION     DAYYHX010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.490"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>281
                     /locus_tag="ACHA6Y_003057"
     CDS             <1..>281
                     /locus_tag="ACHA6Y_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8989051.1"
                     /translation="FTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKHPEHN
                     CTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQ"
BASE COUNT          100 a           40 c           56 g           85 t
ORIGIN      
        1 tatttactat ttgtggtaga gctgggtcta aggggtttaa aaataaaaat ttgaaatatt
       61 tggcaggaaa gcccctagtc catttcgctt tagcaatgat agatatctat caacaaaaac
      121 atcctgaaca taattgcacg acttgtttga atacggacag taatgagtta gtagaattga
      181 cgactaagtt aaatccgtct gtgaaaattg tagaacgtaa acctgaatta gctacagata
      241 ctgtagggaa aattgaagtg atacgtgatt cattcttaca a
//
LOCUS       DAYYHX010000456          281 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.491, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000456 DAYYHX010000000
VERSION     DAYYHX010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.491"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..>281
                     /locus_tag="ACHA6Y_003058"
     CDS             28..>281
                     /locus_tag="ACHA6Y_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8989052.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT           84 a           53 c           56 g           88 t
ORIGIN      
        1 tgaaaacgaa tgcaaggagt gatagctatg atgtctaaaa acaatacgaa tggtagaaat
       61 caatttgcga tgcttacgat tgatgatcta gttcctcaag atcatctcgt tcgaaagatt
      121 gatgccgcgc ttgattttga atttatttat ccaatcgtcg aagccactta ctccgattta
      181 ggtcgcccaa gtatcgatcc agtgattctg atcaaattgg tgtttattca gtatttattc
      241 ggtatccgtt ccatgcgaca aaccattaag gaagtcgata c
//
LOCUS       DAYYHX010000457          264 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.497, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000457 DAYYHX010000000
VERSION     DAYYHX010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.497"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           98 a           43 c           49 g           74 t
ORIGIN      
        1 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
       61 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      121 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      181 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      241 ttttgataaa aaacagtggg ttga
//
LOCUS       DAYYHX010000458          262 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.500, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000458 DAYYHX010000000
VERSION     DAYYHX010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.500"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(3..>262)
                     /locus_tag="ACHA6Y_003059"
     CDS             complement(3..>262)
                     /locus_tag="ACHA6Y_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8989053.1"
                     /translation="SNRFVKDFYKVWDSEVFFLAIEDSLLINLYYSNKNYFKIPATKT
                     RMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT           87 a           51 c           37 g           87 t
ORIGIN      
        1 ctttaatctt tatacctttc tggatcaacg aaagtatact tcaaatagtc ataacgccga
       61 tgatcgctcc gtgcgtctgg cacatcagtc acgatatcaa acaaaaaata cacatctttc
      121 ttcattctgg tttttgttgc cggaatttta aagtagttct tattagagta atagagattg
      181 attaataagc tatcttcgat tgctaaaaag aaaacttctg aatcccacac cttataaaaa
      241 tctttgacaa atctattcga ag
//
LOCUS       DAYYHX010000459          260 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.502, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000459 DAYYHX010000000
VERSION     DAYYHX010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.502"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..>260
                     /locus_tag="ACHA6Y_003060"
     CDS             100..>260
                     /locus_tag="ACHA6Y_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8989054.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALE"
BASE COUNT          100 a           48 c           39 g           73 t
ORIGIN      
        1 ttattacgcc gattgtaaaa ttaagctaga caactaaaaa agtcatttgt gctacactca
       61 aaatagttcc gaccaaagaa ttataaggag tgagtacaaa tgacctatac ccatcttaca
      121 acggatgaac ttgtaataat agagtcttat ttcaaaatga atcaatctgt tgctaaaact
      181 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
      241 ggaaaatcag ccttagaata
//
LOCUS       DAYYHX010000460          256 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.503, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000460 DAYYHX010000000
VERSION     DAYYHX010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.503"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..>256)
                     /locus_tag="ACHA6Y_003061"
     CDS             complement(24..>256)
                     /locus_tag="ACHA6Y_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8989055.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           74 a           38 c           43 g          101 t
ORIGIN      
        1 accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat caaaaaaatc
       61 ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag tgtcttgata
      121 tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc attgtaaata
      181 ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc aagtgtttca
      241 ggaagatttt ttaata
//
LOCUS       DAYYHX010000461          253 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.504, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000461 DAYYHX010000000
VERSION     DAYYHX010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.504"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>253
                     /locus_tag="ACHA6Y_003062"
     CDS             <1..>253
                     /locus_tag="ACHA6Y_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8989056.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWT"
BASE COUNT           80 a           40 c           58 g           75 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga cat
//
LOCUS       DAYYHX010000462          248 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.506, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000462 DAYYHX010000000
VERSION     DAYYHX010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.506"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           91 a           33 c           40 g           84 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggag
//
LOCUS       DAYYHX010000463          236 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.508, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000463 DAYYHX010000000
VERSION     DAYYHX010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.508"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>236
                     /locus_tag="ACHA6Y_003063"
     CDS             <1..>236
                     /locus_tag="ACHA6Y_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFM8989057.1"
                     /translation="YEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHYD
                     EILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYK"
BASE COUNT          106 a           34 c           37 g           59 t
ORIGIN      
        1 ttatgaaaaa aataaaaaaa gcatccaaag ttacttattg aaagaaaaag aaaactatcc
       61 tgttcaacat gctacgaaaa aaaggtggac aactgtccgt tatggtaata gcgatcacta
      121 tatccattac gatgaaatcc tttatctaat gagcatgcca ggttataaaa ataaaatagg
      181 cctagtattg aaagatgaag agattctcgt aaacggcaca attaaaaaat ataaaa
//
LOCUS       DAYYHX010000464          233 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.510, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000464 DAYYHX010000000
VERSION     DAYYHX010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.510"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DAYYHX010000465          232 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.511, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000465 DAYYHX010000000
VERSION     DAYYHX010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.511"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..>232
                     /locus_tag="ACHA6Y_003064"
     CDS             21..>232
                     /locus_tag="ACHA6Y_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8989058.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEI"
BASE COUNT           86 a           44 c           44 g           58 t
ORIGIN      
        1 tacaaggaga gtgaacataa atgacttata cccatcttac accaaacgag cttgtaatga
       61 tagaagcata ttttcatcaa gaaactccgg ttgctatcgt tgcgaagcag cttaaacgtg
      121 gacgccaaac aatttacaat gtctataact ttctcaaatg tggtggaaca gcacttgaat
      181 actttgaaca atacaaagaa aataagcgac gttgtgggag aaccgaaatc at
//
LOCUS       DAYYHX010000466          224 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.513, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000466 DAYYHX010000000
VERSION     DAYYHX010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.513"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>224
                     /locus_tag="ACHA6Y_003065"
     CDS             <1..>224
                     /locus_tag="ACHA6Y_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM8989059.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAYYHX010000467          222 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.denovo.514, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000467 DAYYHX010000000
VERSION     DAYYHX010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.denovo.514"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(2..>222)
                     /locus_tag="ACHA6Y_003066"
     CDS             complement(2..>222)
                     /locus_tag="ACHA6Y_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8989060.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT           65 a           56 c           36 g           65 t
ORIGIN      
        1 ttcatttgtt tcctccttct tcgctttcag ccgagaacat tttgtaggtt cgatttgata
       61 cacccaacac actccctaaa aacgcgccaa aaccagtaat gatgacaaca cagatatctg
      121 tgtactgcca attgagcgct ttaccaacta accccacgaa agtagctagt gcgggaataa
      181 ttaccagtgc gaaccatttt agtacttcga acgttttatt at
//
LOCUS       DAYYHX010000468        12613 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.guided.072, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000468 DAYYHX010000000
VERSION     DAYYHX010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.guided.072"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACHA6Y_003067"
     CDS             complement(134..577)
                     /locus_tag="ACHA6Y_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8989061.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACHA6Y_003068"
     CDS             complement(795..2135)
                     /locus_tag="ACHA6Y_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8989062.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACHA6Y_003069"
     CDS             2404..3300
                     /locus_tag="ACHA6Y_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8989063.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACHA6Y_003070"
     CDS             complement(3450..4391)
                     /locus_tag="ACHA6Y_003070"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8989064.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACHA6Y_003071"
     CDS             4517..5638
                     /locus_tag="ACHA6Y_003071"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8989065.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACHA6Y_003072"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACHA6Y_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8989066.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACHA6Y_003073"
     CDS             7465..7992
                     /locus_tag="ACHA6Y_003073"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8989067.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="ACHA6Y_003074"
     CDS             8203..9264
                     /locus_tag="ACHA6Y_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8989068.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="ACHA6Y_003075"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="ACHA6Y_003075"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8989069.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="ACHA6Y_003076"
     CDS             10177..12159
                     /locus_tag="ACHA6Y_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8989070.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..>12613)
                     /gene="nrdI"
                     /locus_tag="ACHA6Y_003077"
     CDS             complement(12224..>12613)
                     /gene="nrdI"
                     /locus_tag="ACHA6Y_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8989071.1"
                     /translation="AEYAEKMHAEDPVNPEVTLKEIHENSDFTKETEPFFTFVPTYLD
                     GGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNKNFNNQYCLTAKQYAQTFGF
                     PFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         4160 a         2311 c         2617 g         3525 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgc
//
LOCUS       DAYYHX010000469         4349 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.guided.197, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000469 DAYYHX010000000
VERSION     DAYYHX010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.guided.197"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            299..1777
                     /gene="msr(C)"
                     /locus_tag="ACHA6Y_003078"
     CDS             299..1777
                     /gene="msr(C)"
                     /locus_tag="ACHA6Y_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8989072.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1824..2270
                     /locus_tag="ACHA6Y_003079"
     CDS             1824..2270
                     /locus_tag="ACHA6Y_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8989073.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2469..2984)
                     /locus_tag="ACHA6Y_003080"
     CDS             complement(2469..2984)
                     /locus_tag="ACHA6Y_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8989074.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3101..4303
                     /locus_tag="ACHA6Y_003081"
     CDS             3101..4303
                     /locus_tag="ACHA6Y_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8989075.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1311 a          731 c          940 g         1367 t
ORIGIN      
        1 cctaataaaa aaggaggtcg gaaaaatgac tgcatcgatg aaattacgtt tcgaactttt
       61 gaataacaac taaataaatt caaaagacat gtataaacaa caatttcatg gatggagtat
      121 gtccattttt tcgaacgaca gtagaatagg tgaaagctat gacagatttg gtgatttagt
      181 catagttatt tcgttcagag taggaaaaga tgtgatgcag tttttttgca aacagatttt
      241 tctgctcttt tttagtatcg ataattgttg ttttacatga aataggagtg taaaacatat
      301 ggaaaattta gcagtaaata taacaaatct acaagtcagc tttggcaacc agctagaact
      361 atctattgat tctcttcgtg tctatcagca agatcgtata gggatcatag gagaaaatgg
      421 tgtcggtaaa tcgactttgc tcaaactaat agccggtgaa ctttttcccg atcatgggaa
      481 aatccaaaca gagatcacct tcaactacct gcctcaatta acctatctcg ctgaagcaaa
      541 ggacctaaat ttggaattag ccagtcattt ccagttaaga ctggaagaaa cttcagagcg
      601 gaaatggagc ggaggggaag aacgaaagat cgagttgata cgtcttcttt cttcttatga
      661 acaagggatg cttctagacg agccaacaac ccatctagat agaaaaagta ttgatcgact
      721 gattgaagag cttcgttatt attatggtac gctggttttt gttagtcatg atcgctattt
      781 tctagatgag ctagcatcga aaatctggga agtaaaagac ggagaaatcc gagagttttc
      841 ggggaattat agtgcctatc tcactcaaaa ggaattggag aaaaagactc agctacgaga
      901 agcagagtcg atcatgaaag agaaaaaacg attggaaaaa tcgatccaag aaaagaaaaa
      961 acaagcggaa aagttggaaa aagtgtccag taaaaagaaa aagcaacaaa tcagaccgga
     1021 tcggttgtct tcctctaaac aaaaagacag tgtacaaaaa gccatccaaa agaatgcgaa
     1081 aacattagag agaagactcc aaaaaatagg agaaacaacc aagccgcagc agatgaaaca
     1141 aatccgtttt ccagtaccaa aatctcttga actccacagc cgctatccca tcatgggaca
     1201 aaatgtccaa ttggaaagaa gtgggagaac attactggta aatggtgatt ttcagttttc
     1261 tttaggtaaa aaaatcgcga ttgtcggtga aaatggttca ggtaagacaa ccttattaga
     1321 acatatccgc aaacaaggag aaggaatcct tctctctccg aaagtaagct ttcaagtata
     1381 tcagcaaaag ggttatcaaa tgacatctga agaatccatc attcgttttg tcatgagaca
     1441 aacagagttt tcagaatcgc ttgtccgtag tttgctgaat cacttagggt ttgctcagga
     1501 aactttgacg aaaccgttat gtacattaag tgggggagaa gcgacccgtc tgacgattgc
     1561 tttgcttttt actaagccaa gtaatgtgtt gctgttagat gaaccgacta attttattga
     1621 tatggcaacg atcgaagctt tagagaagct aatgcaaata tatccgggaa cgattttgtt
     1681 tacttctcat gattcttact ttgtcgagcg tacggctgat gaagtttatg aaataaaagg
     1741 gcagaaaata aaaaaagtac ttacgagaaa tttttaaatg tgctacgatg attttcgtga
     1801 caaatttaaa ggagtggtac agattgccta caaataaaaa agaaggaata atttttacaa
     1861 caatgatgtg ttttttgatg gtcttgggga tgagcgcgta taatttatgg ctgcatcaag
     1921 atcttcattt atcaagtttg ttgattggtc tagtgcctgg attcgtggtg gctttcattt
     1981 tggatgtctt catcatcggt gtgctggcga aaaaaattgc ctttaaacta ccagtaaatc
     2041 cagaaagcaa ggtccaaatg atcttaacta tttcatgctt gatggtttta ggaatggtga
     2101 catgtatgtc tttattcggt gtgatcatgg aacaaggaat acctgatcaa ttattctctg
     2161 cttatatgaa tgcttggaaa atgaatgtga ttgccgcttt accgttgcag ttgatcatcg
     2221 ttggcccgct ttcaagaagt gtgctaaaag caattcaaag taatgaatga tggaacactg
     2281 ttatttgaaa aaaggccttg gcatgctttt tgtcatagtc tcttgaatgc agttctagaa
     2341 aaatagtgat ccgaacgata ggttttggcc tgtcgttcgg atcgcttttt cttttccaac
     2401 tgtattgctc ttttattgaa gataatttgc tcttcaatag atatttctag aaaggttctg
     2461 tttatccctt actcaaaaaa ctgctgcatt tgttctctta cttgctgaca ccattggagt
     2521 tcctgctgac aaagcataat tctgcgctgg atcacaacat aacgtccaaa ttcttgtttc
     2581 ttgtctagcg agaggtcatt caattttttt tgattttttt gcatgatttt gttcagctgt
     2641 tgttctctag tgaatagcag tccagtagct gttgttttat cgagcgtcga aaaagcatag
     2701 atttttgcta gaaattcatc tttttgcgta ggagcgggag tttcctcttt gatccattcc
     2761 tctacaagag cacgtccttc atcagtcaag tggtagatct ttttttgaga gtgctcgtct
     2821 ttgtccaaaa cggtcacata accctgctgg tctaacttgg caagcgttgt atagatctga
     2881 ctgtgatggg cttcccaaaa aattgtgatc agctgcttca attcataccc agaacgtggt
     2941 ttggataaaa gcacgcataa taatatatag gctaaagtat tcataaaaat cctacctctc
     3001 gtcttatttt ctttattata tcacagtaaa gtttccacgt tttacatgtg catatttaca
     3061 tgtaaaataa atcatgtatg atgctaatag agagaaaggg atggaaatat tggagaatca
     3121 aatagatata caagaacaaa caaatgaaga aacaaagggg aaaagttcag cgctagttgt
     3181 agcagctacc tgtatgatcg cttttatggg agtgggtcta gtagacccaa tcttaaaaac
     3241 gattgctgca cagttaaacg ctacgccagc tgaaacgacc ctgttattta ctagttacat
     3301 gttagtcact ggtgtggtga tgctatttac aggtttctta tctagtcgga tcggtttgaa
     3361 aaaaacattg atgacaggat tgtttatcat cgttgtattc gctggtcttg gcggattatc
     3421 tgggaatatc ggaatgctga ttgggttacg tgccggctgg ggtgtcggga atgctttatt
     3481 tgtctctact gctttagcaa cgatcgtcag tattttagtt ggtaacacag aaaaagcaat
     3541 catgatgtat gaagcagcac ttggctgcgg aatggctgtt ggccctctag tcggtggggt
     3601 cttgggcagt ggttcatggc gttatccatt ttttggggta gcatttttga tgttttgcgg
     3661 ttttatagcc ctagcggtac ttttacctga aactgagaaa ccaaagagaa aagtaggcct
     3721 ttgggaaggt gtcaaagcgt taagcaatca taaacttcgt tcagttggac taatcgcatt
     3781 gttatataat ttcggattct ttactttact tgcgtatgcg ccatttttat tgattggcta
     3841 ttctgaatta gaagtcggtt ttgtattttt tggctggggt gtgcttctgg ctattgcttc
     3901 gattttcgtt gcaccggctc ttgagcggaa gtttacgact tatcggacaa ttttagcagc
     3961 atttgtctta ttcattattt gtttgctgtt attaggagct ggaacttcta tgccagtcct
     4021 tgtaccgatc agcattgtgt tagcaggatt tttccaagga attatcaata cactgttgac
     4081 gacgattgct atggaaatac caggtctgca acgaaacgtt gcttcgtcca gctatagttt
     4141 tgtccgcttt ttcggtggag ctttagcacc atttattgca ggtgtgattg gtgaacatat
     4201 tggtgaaagc tatgcgtttt attttgcagg tgccattgtt cttatcagtg tatgttgtat
     4261 catttatcac cgtaattatt ttgctacaaa tgaagaagaa taagaaatca gaggttgtga
     4321 aaaagctgtc ctgcatcaag tcataagaa
//
LOCUS       DAYYHX010000470         2957 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.guided.245, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000470 DAYYHX010000000
VERSION     DAYYHX010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.guided.245"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..493
                     /gene="dfrG"
                     /locus_tag="ACHA6Y_003082"
     CDS             <1..493
                     /gene="dfrG"
                     /locus_tag="ACHA6Y_003082"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8989076.1"
                     /translation="VSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILGRK
                     NLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIYNL
                     FFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYERKN
                     LLS"
     gene            complement(565..2517)
                     /locus_tag="ACHA6Y_003083"
     CDS             complement(565..2517)
                     /locus_tag="ACHA6Y_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8989077.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2524..2760)
                     /locus_tag="ACHA6Y_003084"
     CDS             complement(2524..2760)
                     /locus_tag="ACHA6Y_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8989078.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT          886 a          535 c          491 g         1045 t
ORIGIN      
        1 agtttctttg attgctgcga tggataagaa tagagtgatt ggcaaagaga atgacattcc
       61 ttggaggatt cccaaggact gggaatatgt taaaaatact acaaagggac atccgataat
      121 attaggtagg aagaaccttg aatcaatcgg aagagcctta cctgacagaa gaaatattat
      181 tctgacgaga gataaggggt ttacctttaa tggttgtgaa attgttcatt caatagaaga
      241 tgtttttgag ttatgtaaaa acgaagaaga aatttttatt ttcggaggag aacagattta
      301 taatttgttt ttcccttatg ttgagaaaat gtacatcaca aaaatacatc atgaattcga
      361 aggagatact ttttttccag aagtgaatta tgaggaatgg aatgaggtat ttgcccaaaa
      421 agggataaag aatgataaaa atccgtataa ctactatttt catgtatatg aaagaaaaaa
      481 cttattgagt taacgggtgc gttaattgaa gaagaacagt aaagataaag cacaagagct
      541 tgggattact ccaagctctt tttgtcattt gaaataaatt atatctttcc aatcaaattg
      601 tttatctgta atccctaatc tttgagcagg tgtcaaaacc tttttatccg ctgatttaaa
      661 agggcgacaa aagttgtagt aagttcttag aattgtaatc gcaaattgag catacttcgg
      721 attgaagtta gcgtaaatat agctcttctt atcaccacga gctgttgtta atggtctttc
      781 aagagatgat atataacgtc ttatctgttg aataaagcta ttggtcgaat ggtcattaac
      841 atttaatagc atctttgcga tttcgtttgg ttccagagca gataaatccg ttctacaatc
      901 aactgaataa aatcctttat ccttagtggc taatggatgt ttgatagggt tatcggcata
      961 tttgcgatat ctttctccat ctttactaat agcctcttcg tggaattgat gacttgttag
     1021 taattcagtt agataatgag atgccaaagt ccgtagaaac tttgttttga atcctctgat
     1081 atctccccaa tcgagcagtt ctgcttttgc ttgtccgaat tcttttaagc attgttttct
     1141 gcttttcgtc ttgttaactt ggcaaataaa atgatgtgca tcgctcaacc ttaattcttt
     1201 cgtgaatact ctataaaagg cgtttctaat agagaagtca tcatcactaa tcattcgcca
     1261 ttcgtcggca ttaaccattt gtttaattaa ccaatagtga gcagttgtag tataaggagc
     1321 atcaacattt aaaccatcga caaatctact cctattagta attttcccta attcgtgaag
     1381 ataagcattc ctattttcgc tatcgttagc ggatggttct tgtggataat aagaccaatc
     1441 taacctatca tttttacgac taaaagtatt gagatggtct tctttgaatt ttcttgtatc
     1501 ttcatttaat tcatccattg aaatattcca atcataggca acatcagaac ggaacacata
     1561 tcttgataag acctcagcag atacaactat atacgtttgc atattaaggt cttcaaaccc
     1621 cgtatatttc tttgaacctt gtcctttctt acggacgtta tttaggtagt agtgcatctt
     1681 gtccgtatta agccacatct cgttaaattt ctttgtttga aggggttgtg tctcatatcg
     1741 ttctaaaaac tccaaacaac gcctatacag ccactccagc ttatgatagt aggttccaac
     1801 tccaattcct aaaatatcac aagtacgact gactgaaacc cttccaacaa ccatcttagc
     1861 gaacattgga agaatggtgt tcttttgttg gtgataagta gtggtttctt ctctttttgg
     1921 tagaacatta gtgaacttct tacacgcctt acattgatag cgttgagatt tgcccctact
     1981 tttccccctt ttgtaaaatt gcttcggctc attgaatggg gttgattctt cttccgagca
     2041 gccttcttta tgaaattgat aatctggctc aacatcttgg atgctattga tttctataag
     2101 gcgtttaatt tcttcaacaa cagaccaatt agataaaggg gttactgtac agttttgcga
     2161 tacacctctg ccaataggat tgtcattgca gtacatacct ttatgacctt tatctttgga
     2221 actaccagtc attttatatc gacttggttt cccttttaca tctttgaatt tgtgctgttc
     2281 tttcccaaaa ttacaacaaa acggattgat gcagtaatta tattgaatgg gatattcaga
     2341 accgttccat ttaaaagacg ttggcaagaa caacattttg ttataccttg ttgcaaatcg
     2401 ttttggagtt aataaatcat attgtttagc cctatctttt aattcttcgt ttgaaatcgg
     2461 aattttaaca tctacaattt catcttcctt cgtggctaat cttttaagct tggtcattgt
     2521 ctatcaaccc actctatctt tgatacccat tttttcaaca atccgcttat tacttcgctt
     2581 gttggcaatc cattcaataa acttcttatc gtctaaaatg ttcaataaga attcatccac
     2641 tgcttcgctt tcagtacgct ctgcatattc agcgtattgc ttaactatca cccttacttg
     2701 ctctgatatt tcccaatcca cagacttagc gttcttattt tttggctcaa gaaattgcat
     2761 aggaaaatgc accccttttt gcttagtaaa ttgtaaccat tttaacacac caatttgcat
     2821 tcctacaata attatttcac gttcgcattt ttatatacat tttaacttac attttgtggt
     2881 gtaaattaac aacaaacgtt gatttaacaa taaaaaacga gtatcaaatt cagttgcaat
     2941 ttgatactca tttaggt
//
LOCUS       DAYYHX010000471         1908 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.guided.297, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000471 DAYYHX010000000
VERSION     DAYYHX010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.guided.297"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..537
                     /locus_tag="ACHA6Y_003085"
     CDS             <1..537
                     /locus_tag="ACHA6Y_003085"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8989079.1"
                     /translation="DIINETKLSKGAIYHHFDSKEDILKAIFQRIGNENAVIFAKIRD
                     NDSLNGLEKLREIFKTAVFNSNQSVLLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEP
                     VLEQGIKDKSLEIENSHEIAEVIMVLSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIG
                     IDTLLDEETISAFIQFCK"
     gene            644..1882
                     /gene="mef(H)"
                     /locus_tag="ACHA6Y_003086"
                     /gene_synonym="mefH"
     CDS             644..1882
                     /gene="mef(H)"
                     /locus_tag="ACHA6Y_003086"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8989080.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
BASE COUNT          609 a          268 c          330 g          701 t
ORIGIN      
        1 gatattatca atgaaacaaa actttctaaa ggtgctattt atcatcattt cgattcaaag
       61 gaagatattt taaaagcaat ttttcaaaga attggaaatg aaaatgcagt aatatttgct
      121 aaaattagag ataacgatag tttaaacggt ttagaaaagt taagagaaat atttaagaca
      181 gcggtattca attctaacca aagcgtttta ttgacagttt ctcctcaact attaaacaat
      241 cctcgttttc ttgctatgca aatagaacaa atgtatgaaa ttgtagtacc tgaatttgtt
      301 gaacccgttt tagaacaagg tatcaaggat aagtctttgg agattgaaaa ttcacatgaa
      361 attgcagaag ttatcatggt attaagtaat gtatggctca acccattagt tgaaatgacg
      421 gataaagagg gtatggaaaa acgttgcaat acattcaatg cccttttaaa tggaataggc
      481 atagacacat tattagacga agaaacaatt tctgcattta tacagttttg taaataactg
      541 tatttgttat ccctatacga tagactgctc tataaaagag cagtttattt aaaccattat
      601 ataaaccgac ggtctgtttg attaaataaa aaggagagat tttatgcaag cagaaaaaaa
      661 actttttaat cgtgatttta tacttgttgt tataggacag ataatttctt tatttggaaa
      721 cgctatccta cgatttgcat taccgtacta tttgttaaaa gaaactggtt catcagcttt
      781 gtttggtatt gctacagcct gctctttttt acctatggtt attttgtcgc tggtaggtgg
      841 tattattgct gacaggacaa ataaaagaaa cattatggtc atactagatt ttgtaacagc
      901 aatcataatt ttagtatttt atttgtcatt gggaaaaatt cctatcatac ctttgtttat
      961 tgttgctttg atgttacttt atggtatttc tggtgcatat caaccagcgg tacaagcaag
     1021 tattcctcta ttggtatcat cagataaact taccgtcggt aatgctataa taaatcaagt
     1081 aaatacttta gcaaacttta tcggaccagc tataagcgga gtagtttttc aattttatgg
     1141 tatcaatccc attttaattg ttagtattat atgttttact ttttcagcca ttatggaaat
     1201 ttttatccat attccacacc aaaaaagacc tcgtgaagta ggtgtaattg cagttgctaa
     1261 aaacgattta aaagatagtt ataccttttt gaaaaaggag aaacccattt ttatatccgt
     1321 aatctttttg gtttgcattt tcaatttggt tttaagtgct gttttaattg ttggtctgcc
     1381 tatcttaatt tctgaagttt taaaaatgcc agatacacta tatggttttt cacaatcagc
     1441 attatcatta ggtggtttat gtggtggtat tttaacagct cttgttgctt caaagttaaa
     1501 actaaaaaac ctttatacct tgttacttat ttgttctgtt tctgttttac ttatgggaat
     1561 ttcgctattt ttagaattgc ctgcaatagt ttcctactgg gtagttacag tgatgtgctt
     1621 tgtggctatg ggtgtatcta ctatgtttgt tgttcaaatt tacactcttg ttcagacaca
     1681 aacaccaccg caattagtag gaaagataat ggctacgctg gtagcagttg ctatgtgcgg
     1741 tcaacctatt ggacaaatgg tttacggtat tttattcgat gtatttatac aagaaacatg
     1801 ggttgttatg tttgtagctt ctattgcttc aatcatgatt tcaatatatt caaaaagaat
     1861 ttttcaaaag ttagagatat aatacaaggg gttaaatagt atgttagg
//
LOCUS       DAYYHX010000472         1146 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.guided.355, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000472 DAYYHX010000000
VERSION     DAYYHX010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.guided.355"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..363
                     /locus_tag="ACHA6Y_003087"
                     /pseudo
     CDS             14..363
                     /locus_tag="ACHA6Y_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(635..925)
                     /locus_tag="ACHA6Y_003088"
     CDS             complement(635..925)
                     /locus_tag="ACHA6Y_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8989081.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          359 a          226 c          176 g          385 t
ORIGIN      
        1 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
       61 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      121 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      181 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      241 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      301 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      361 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      421 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      481 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt
      541 gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag
      601 cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga
      661 ccttttttct tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca
      721 catgatagga tctcacaaca aattgctttc tattattata ataacctgta acgactaact
      781 tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga
      841 ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat
      901 ctactgttcc atattttttc tccataacct tcacctcaat accattatac gaacatacat
      961 tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt
     1021 aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca
     1081 gtatagattt aagtagttca gttttggagt acaaaaagtg aaaatgtaaa cctaaatgag
     1141 tatcaa
//
LOCUS       DAYYHX010000473          748 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.guided.390, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000473 DAYYHX010000000
VERSION     DAYYHX010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.guided.390"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..683
                     /gene="aac(6')"
                     /locus_tag="ACHA6Y_003089"
     CDS             135..683
                     /gene="aac(6')"
                     /locus_tag="ACHA6Y_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8989082.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          274 a          110 c          166 g          198 t
ORIGIN      
        1 taatttttta ttttataagt aagataaata atgaaagaat agagatactg attggtagct
       61 aaagattttt gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac
      121 aagggggaag acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc
      181 agctttctga tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag
      241 aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt
      301 tagtaggatt tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat
      361 tagttgtaga aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag
      421 aaaaagaagt agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc
      481 atggaacaac gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca
      541 tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag
      601 gtgtcttacc aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta
      661 ttcctcgacc aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga
      721 aataagaagg aatttacgaa aattactt
//
LOCUS       DAYYHX010000474          682 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.guided.399, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000474 DAYYHX010000000
VERSION     DAYYHX010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.guided.399"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..654
                     /locus_tag="ACHA6Y_003090"
     CDS             <1..654
                     /locus_tag="ACHA6Y_003090"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HFM8989083.1"
                     /translation="SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFL
                     RQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVF
                     EGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFG
                     EDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          288 a           46 c          117 g          231 t
ORIGIN      
        1 tcgtatatta gtgatgaatt atctatacta ggttataaag aaattaaagg aactttttta
       61 acaccagaaa tttattctac tatgtcagaa gaagaacaaa atttgttaaa acgagatatt
      121 gccagttttt taagacaaat gcacggttta gattatacag atattagtga atgtactatt
      181 gataataaac aaaatgtatt agaagagtat atattgttgc gtgaaactat ttataatgat
      241 ttaactgata tagaaaaaga ttatatagaa agttttatgg aaagactaaa tgcaacaaca
      301 gtttttgagg gtaaaaagtg tttatgccat aatgatttta gttgtaatca tctattgtta
      361 gatggcaata atagattaac tggaataatt gattttggag attctggaat tatagatgaa
      421 tattgtgatt ttatatactt acttgaagat agtgaagaag aaataggaac aaattttgga
      481 gaagatatat taagaatgta tggaaatata gatattgaga aagcaaaaga atatcaagat
      541 atagttgaag aatattatcc tattgaaact attgtttatg gaattaaaaa tattaaacag
      601 gaatttatcg aaaatggtag aaaagaaatt tataaaagga cttataaaga ttgattatat
      661 aatatatgaa aagctattat aa
//
LOCUS       DAYYHX010000475          565 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7901
            SAMEA115709349-rid22597063.guided.418, whole genome shotgun
            sequence.
ACCESSION   DAYYHX010000475 DAYYHX010000000
VERSION     DAYYHX010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709349
            Sequence Read Archive: ERR13199067
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290433.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290433.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:29:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 37 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709349-rid22597063.guided.418"
                     /isolate="VRE7901"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>565
                     /locus_tag="ACHA6Y_003091"
     CDS             <1..>565
                     /locus_tag="ACHA6Y_003091"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8989084.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLV"
BASE COUNT          224 a           53 c          103 g          185 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagtt
//
